<?xml version="1.0" encoding="UTF-8"?>
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<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJY9</accession>
    <name>A0A5B0LJY9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJY9"/>
    <dbReference type="OrthoDB" id="10298981at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="467" mass="53579" checksum="7365CA3E4BE9E208" modified="2019-11-13" version="1">MIISDQDRLTVSDLLTPSLLLTIVVSTLNFKVHSFDSLLSRLRLRFVMPPPRDPRFRLPSKKKQSQKLKDKAVNSDKAAKKKSTNKTVPDTKKQTTPLSDVRSPLPPPSEHPSDDEMDYDGDKDRSQDDRIIEDENDLDKRPSTIDPVIDELNQQFHQEFGQSQPDTFSSMTRPAGPQPSFHLEDLKNLRRYTKLDNQHFLEAFTILNEFERIQEGSQVQPQAPKDKSDEISTDGFAYSNALKNRVRDITKAALLRPHLDQYTRGKWKPGTNIGYQQDLMALPLEFRKTHFPAAMEKDCEVKEQFFKMVREMHRRIRLAIRERLLKNIINSKGDLIEEGPVPLLCDIARAIFRYLHPAEATMTDADVDKAIPVLWYGQIGHLRLQTVDLLVHSQFKKVSQWALIDDKINEVKARGTDYRAAFGKAVLVKDEALFGQGKTFVEILEDDEENIAMPNEDEIQTHIQPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKJ3</accession>
    <name>A0A5B0LKJ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_014332</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKJ3"/>
    <dbReference type="OrthoDB" id="5580488at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22147">
        <property type="entry name" value="F-box_SpPof1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000775">
        <property type="entry name" value="BnaA09g28200D protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051075">
        <property type="entry name" value="SCF_subunit_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872">
        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872:SF9">
        <property type="entry name" value="UBIQUITIN-BINDING SDF UBIQUITIN LIGASE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="154"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="342"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="389"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="429"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="468"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="520"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="607"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="647"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="216"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="277"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1064775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="683" mass="76214" checksum="08180F29DAA0EC42" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKM1</accession>
    <name>A0A5B0LKM1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018860</name>
        <name evidence="3" type="ORF">PGTUg99_007342</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000217">
        <property type="protein sequence ID" value="KAA1122768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKM1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="62" mass="6920" checksum="AA830B07082AAA90" modified="2019-11-13" version="1">MHKQSNFIDMRSLSGGRWLKVRFASSSAQPAEANQAIQPSTKKNNTQPQRTPPNPINTPTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLG9</accession>
    <name>A0A5B0LLG9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type phospholipid transporter</fullName>
            <ecNumber evidence="3">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PGT21_025287</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 24">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000087">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF180">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="650"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="699"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1317"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1345"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1396"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1431"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1459"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1492"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="255"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="19">
        <location>
            <begin position="475"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="21">
        <location>
            <begin position="1283"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="87"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="144"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="411"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1130"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1572"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1641"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1715"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1865"/>
            <end position="1896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="411"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1132"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1149"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1582"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="1604"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1660"/>
            <end position="1671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1672"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1729"/>
            <end position="1738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1763"/>
            <end position="1772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1812"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1865"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1859"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAA1065115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1916" mass="214419" checksum="9CE3948ED068FADE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLY3</accession>
    <name>A0A5B0LLY3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_017865</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLY3"/>
    <dbReference type="OrthoDB" id="2908607at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="156"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1064870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="261" mass="29103" checksum="8659A509B5E73FB1" modified="2019-11-13" version="1">MSLSSSLRNGNLDKWKLFNLDGSSLTPSTNLGLQPGQRSAALTPDLLPMAPTSLYFTSRTNWPMNPTRVSHELEFLGTPTSNSSKNSDPVTSLASCYNHQHRSKASDAGHKHFVSTPNYLAPENILGIGMDEMVDWIPSLAPTQVIRQPRLTTDLGHQPWHLPAIPQPQPSQLQNRQREQNPRELTNRISPNTQIFVELVFKGPKKHVEYHRAMHRLGAQGEAEVKSHPFLANINWVNLFKSEASFVPSLIEQSLALFGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNH7</accession>
    <name>A0A5B0LNH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014274</name>
        <name evidence="1" type="ORF">PGTUg99_014013</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNH7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="195" mass="21933" checksum="777217B77852B53B" modified="2019-11-13" version="1">MWIPDSDRPFGCNFTGIKPSDKRTAEVLEAIGPLDHAVKRLWFSLLKCRTIDSDGLGRGLITEIRPGRPTRRDLGVSSRQSFTDDPSTACIFIDRSRRVEGKSQEIVACTTTIQSSGWLYPYALSLPDPLLWNFFLTRELLAALLHLLWLDSEILDTWLAPEVGRCRPPGISASPIPFPGLWSLWTIHPFPSSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LP75</accession>
    <name>A0A5B0LP75_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004839</name>
        <name evidence="3" type="ORF">PGTUg99_022461</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1080022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LP75"/>
    <dbReference type="OrthoDB" id="10351653at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366022" evidence="1">
        <location>
            <begin position="21"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="105" mass="11494" checksum="D6EED1A0D9E5FEB2" modified="2019-11-13" version="1">MHVFGIALVCLAVVCRFTTAFGCYPNRPQQVCRVQLGELVWYVRADYNTVKIDQEPLHFSVTAGTECVDTTGHVVGKACCPANAIPVKSYINGPHFKELGCGAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQ90</accession>
    <name>A0A5B0LQ90_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ras GTPase activating protein ira2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">IRA2_5</name>
        <name evidence="1" type="ORF">PGT21_008692</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQ90"/>
    <dbReference type="OrthoDB" id="10603852at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="145" mass="16434" checksum="629F0068C3ACAD70" modified="2019-11-13" version="1">MTFLSRRSWGTNHYRNSCWIRGTMGQIEEVCGLRFNNGEAGYAPDGAIYFGFALASSMIKGLRHAQIAQDAEEFLELLLIQATNRMNEEGHHHGGSRMSPEALPYFVVLLHIAARTGKLLQLFALARCRRTRALLWLHHQSTLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQK0</accession>
    <name>A0A5B0LQK0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021641</name>
        <name evidence="2" type="ORF">PGTUg99_009762</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000283">
        <property type="protein sequence ID" value="KAA1111446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQK0"/>
    <dbReference type="OrthoDB" id="2500141at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="101" mass="11532" checksum="A5110C805BE2C8FB" modified="2019-11-13" version="1">MENSTRSDASNIYRMIMVHDMASKTPAYEQYLRDEAKKDLYVNTEAKMAALKDLAAKQFAPKTSINHPRLWILRMRDDAALQNLGTPSIPRSDHKGKTVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQY4</accession>
    <name>A0A5B0LQY4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQY4"/>
    <dbReference type="OrthoDB" id="10378475at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="11122" checksum="6AD951E45BE13538" modified="2019-11-13" version="1">MTDNLETNKSKTLTTNQTSTIKTTDSSMDKINAMILKTAIEAIPLLTQDNYTMWNNRVLNFLELQKLKDTFLQEDEDKLSTDDELHARTILTSKLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRZ5</accession>
    <name>A0A5B0LRZ5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_013098</name>
        <name evidence="14" type="ORF">PGTUg99_010010</name>
    </gene>
    <organism evidence="14 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="14 17">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA1 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1066906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRZ5"/>
    <dbReference type="OrthoDB" id="1920692at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017384">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17098:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17098">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MWFE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15879">
        <property type="entry name" value="MWFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1066906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1083973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="172" mass="19301" checksum="80E84F33D1E1D9C4" modified="2019-11-13" version="1">MLTEQAWTTWATRSQLGCILVGIGWTRTAWSSAFPRVTSCLAPRHPGGGSVFVFLRSDAAPVQFHKPSRRRGLPYEKPSISTKSSKLMPMPWETFIPLGLLTVMFGASGTLLNAARRFQNDGKPPDYTVDKWDEMMQERNRRLTGSLRGQCVEEKAPLEFQTSSAWPVEKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSB1</accession>
    <name>A0A5B0LSB1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSB1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="118" mass="12946" checksum="6293AA6A08765CFA" modified="2019-11-13" version="1">MVRIVKTNHVQFLDSKKDSQAQSSEEELTVFEEPEDSVNDSAPSSSNYEEEPIPTSADTLSISLEELDQTPNSNDDDSEVEDALVPHQAEEGRSLRNQTSKVKPVVSLRASQAGQKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUB1</accession>
    <name>A0A5B0LUB1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1068022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUB1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022927684" evidence="2">
        <location>
            <begin position="20"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="251" mass="27909" checksum="D5AB2D683E9003B7" modified="2019-11-13" version="1">MRLHSNLLTQSAFWYFAWSAALVQSALRHDSTKEFEHFRPDLDSSEVVLKQGLKRQADNRFDTYKEDEDSALGGPDYNQVLNLSLAPFGPGNQEDLVLLKASKRQKTVGNDQASGSRLQPSFQPLDLSLGITHDEMNQPTGLNQGSTSHTIHPLSFEAQDALPQTGWIFEHLQSGLQLGEGQDHIVTEPMFTDSSSSQVNHSSHDGVYHSISPKDGLQIADDHKDKLAPPYDQNNENDSVFDNGHKHPNPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LV43</accession>
    <name>A0A5B0LV43_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ras GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RAS1_2</name>
        <name evidence="11" type="ORF">PGT21_023132</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LV43"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04138">
        <property type="entry name" value="H_N_K_Ras_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000080">
        <property type="entry name" value="Ras-like GTPase Ras1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1067959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="273" mass="30519" checksum="E3AD2E8DA08D2A47" modified="2019-11-13" version="1">MLKLAGVGTKPRLTEIKRGCHAHQHVGPQEGQLLSQGPVPSWGTCYQSAQGLKLAQRDLGMCQAQFLREYKLVVVGGGGVGKSALTIQFIQSHFVDEYDPTIEDSYRKQCVIDEEVALLDVLDTAGQEEYSAMREQYMRTGEGFLLVYSITSRNSFEEISTFHQQILRVKDKDYFPVIVVANKCDLEYERQVGAHEGRELARHFGCRFIETSAKQRINVDEAFSSLVKEIRRYNKEQTISRPGPNGPGNGPNGFQEEGEQFQSSGCCSGCLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZC3</accession>
    <name>A0A5B0LZC3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EKC/KEOPS complex subunit CGI121</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">EKC/KEOPS complex subunit cgi121</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_004798</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. CGI121 acts as an allosteric effector that regulates the t(6)A activity of the complex. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. CGI121 is not required for tRNA modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the CGI121/TPRKB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZC3"/>
    <dbReference type="OrthoDB" id="329139at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2380.10">
        <property type="entry name" value="CGI121/TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013926">
        <property type="entry name" value="CGI121/TPRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036504">
        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011465">
        <property type="entry name" value="PRK14886.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15840">
        <property type="entry name" value="CGI-121 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15840:SF10">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
        <property type="entry name" value="CGI-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143870">
        <property type="entry name" value="PF0523-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1070237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="196" mass="21638" checksum="E0ABEA25A6444C98" modified="2019-11-13" version="1">MNVWLFEQVKNTAEIKKALIQASQTNDEAEQSRLDWAFIDASMITSRQHLTTAVCQALVTQSHGALKTKTLHSEILWTLSPGTNIMEAIKKFGLGPQTNALLLVKLQPIEGDNQPSKEALNQAAQALVDGTLVKLDDHLGKSIVNWKELRKIYKLNDDPVIKGLEEQSKRTASDVEWHAMIDQLATATVALKSVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2V1</accession>
    <name>A0A5B0M2V1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016370</name>
        <name evidence="3" type="ORF">PGTUg99_021497</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2V1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137124" evidence="1">
        <location>
            <begin position="21"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="171" mass="19348" checksum="8C853DBB3F10B6F7" modified="2019-11-13" version="1">MHSPTFLIVVLLAVLSGLRALPHRWYEVPVPEELTEPLLRPGATELPDEETIQEATPVETVLPPLQPKELWLRMVKEHHPDVPNPEVHTFKLVSNHKRCTATIWSTRRPCRGVVNGDSFKCRKCGGLTAVHWNPCPYNHKTGIYDGAVGRDKDVEEEDKPIHQVAHTTVIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3V9</accession>
    <name>A0A5B0M3V9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3V9"/>
    <dbReference type="OrthoDB" id="8962729at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="231" mass="23987" checksum="3DD4A7E6D2007D9C" modified="2019-11-13" version="1">MQLHTAPTQPIHPAQSNSMQLHTAPHSSHSANPSSSTQLHAAPYSSTLLLQHSSIQLRTAPLSSIQLHAAPHSSIQLPLSQSIQLNPAPLSSIQLHAAAHSSHSANPSSSSQLHAAPYSSTLLLQHSSIQLHTAPSSSTQLNPTPCSSTQLPLSQSIQLNPAPLSSIQLHAAPHSSIQLPLSQSIQLNPAPLSSIQLHAAPHSSHSANPSSSHSASPSSSAQLIPAPRSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6J6</accession>
    <name>A0A5B0M6J6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">T-cell immunomodulatory protein TIP C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_012899</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6J6"/>
    <dbReference type="OrthoDB" id="10022113at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057089">
        <property type="entry name" value="C2_TIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024881">
        <property type="entry name" value="Tip"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13412:SF0">
        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13412">
        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23122">
        <property type="entry name" value="C2_ITFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13517">
        <property type="entry name" value="FG-GAP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="646"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="675"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="T-cell immunomodulatory protein TIP C2" evidence="9">
        <location>
            <begin position="539"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1071610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="738" mass="80392" checksum="B3824383CD4B8AB8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MG18</accession>
    <name>A0A5B0MG18_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MG18"/>
    <dbReference type="OrthoDB" id="10272544at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022948973" evidence="1">
        <location>
            <begin position="23"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="237" mass="26606" checksum="BA0357D09A7AD793" modified="2019-11-13" version="1">MQIRQLITLLGLSLSQTPPRAAAPTSPESIKSLVLDITEPHVPVKGSTTSSKYNDAIHIDLHAGPTQAIKNYDRIFDKGGKLKEFADSRAAYDDTFRDVKLMAYPAKMERRLELYRNDMFSRMLDTKLTDEEVKKIHSGVKETLEKNINSRTEDFMWDNKHFASPAAGKSSKAQVELEYMPRNKIIEELNKTDLPGISTPRPLTGEALKGFRQFMEGHEKALLNPDGPKLVGPTPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGS1</accession>
    <name>A0A5B0MGS1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030480</name>
        <name evidence="2" type="ORF">PGTUg99_029911</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGS1"/>
    <dbReference type="OrthoDB" id="2499640at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="117" mass="12455" checksum="7C6AAAC9237A2F73" modified="2019-11-13" version="1">MRTVHESDYPICPIGAPGRDFSHHRRPPPKPVAKPRPTLPSSGSARPQGMVFSIDDLPQNLKNIGGTGLPSAANRLPAPLSPQARASRAKGVIFNVDELPQDLKELAAAHATKAKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIB8</accession>
    <name>A0A5B0MIB8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">U4/U6-U5 snRNP complex subunit prp31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PRP31_2</name>
        <name evidence="12" type="synonym">PRP31_1</name>
        <name evidence="11" type="ORF">PGT21_015094</name>
        <name evidence="12" type="ORF">PGTUg99_025849</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIB8"/>
    <dbReference type="OrthoDB" id="4771285at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000002">
        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein Prp31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000003">
        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027105">
        <property type="entry name" value="Prp31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019175">
        <property type="entry name" value="Prp31_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09785">
        <property type="entry name" value="Prp31_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="265"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="385"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="448"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1076665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1126828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="561" mass="61022" checksum="5493522173F7B339" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MID8</accession>
    <name>A0A5B0MID8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_027950</name>
        <name evidence="3" type="ORF">PGTUg99_019885</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1075836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1091188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <end position="18"/>
        </location>
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        </location>
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            <begin position="51"/>
            <end position="72"/>
        </location>
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            <end position="195"/>
        </location>
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            <end position="214"/>
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            <end position="225"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="248"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1091188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="276" mass="27075" checksum="A938E696E70F4A9F" modified="2019-11-13" version="1">MQFFTLLLCALMSSSALTAQPGRGSIRQRRSLDHEIKENWMIRKRDGDAPGAPPTPAPGAPPTTAPGAPPTTAPGAAPTTAPGAAPTTAPGAPPAGGSAGSTAAAVNILSTVDPADLKNLITEFLNSPVTPGAPPTTTKVGKTKALEMYAKVEEAQKAGQMAPLSPRAAQLFLRIVKRLEGTVTGASGAGASAGKSSKKARRAAPPPGGAPAPTPSTAGSTPGGKNMKDLLKAFIDTLPELTPGAAGGSPSPAPGAGAAPASAPPSTGARAAKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJ11</accession>
    <name>A0A5B0MJ11_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJ11"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="242" mass="26184" checksum="16FF8ED51F18818A" modified="2019-11-13" version="1">MQASQNPWAPPAQTSNAQNPTGQVHTNTVDVDQAIQQALDRQAAKHSEEMANLAKRFEAKLLGKGKGTSSKKKNPPSTQANQPSTSTSSKGKQSTVPKAPPVSTPQRIDRAASAPPQSVNVTPKNKSKGPLKSVRERIAESKGPSPKRHPQQMQTGDFPSEFTTTKILWGLLKESSVPKAPELRDLQEFYNRFSNDAQVQAAASSTQSPGIINSSEVQCFKDVQGGRMKLGKSIIHIPDNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML95</accession>
    <name>A0A5B0ML95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML95"/>
    <dbReference type="OrthoDB" id="2503349at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="254"/>
        </location>
    </feature>
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            <begin position="331"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
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            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
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            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
    </feature>
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            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="409" mass="44744" checksum="7F56E75A3BCED3C4" modified="2019-11-13" version="1">MHCKPPSVSNFEAQTPTTEAPSPSVLSEVSTDTNKEEPTPFNPVVEGLFNQANNLLESVTLAADSGLRKHLSLQCLPLPDQEEPTLKLPQLQAADDPAPPASPAKNKPRVENPLDSKTATIPQAKKSIPPKKADPISSKDASPTVSKKSITKPSPLFVPAKPPTRLISTQPSEKARKKQTISVVIPMSSPSRRPAPSIADPPITIQPPVPNFPQAPVSKTNPSQPSDAMDVDQPEDLDPNEGADPSMDIEPTKLNAVEDQACRIYRQQYHMYETAKKANQHADATRALTECQRSYRNISKKLTWQFALSVNNGWNPFKEAKSAKLLAKLEGRSQPTASGSRPSGSYTSSSNQRGKRPAETDLNKLGKIPKLDTSWRETMAAARALQQARAQILEELRKEEEASKRRKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLX3</accession>
    <name>A0A5B0MLX3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLX3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="114" mass="12557" checksum="956FE616E3FAAD44" modified="2019-11-13" version="1">MPKITEEDKDGNKGDDEDDDANNGRSDCTDDEKENSDDDNNDDDNDNSTEDEGENGKKDGKKNGKKDGKKDGKKDGKKDGNKSSSKANRNDSNDLNELTTAMNKNLSEEQPEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNM7</accession>
    <name>A0A5B0MNM7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012456</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNM7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="67" mass="7305" checksum="F24D4FC8C1EEB261" modified="2019-11-13" version="1">MSKDGHLAIEMAKAQIKFYLVAPIETRKEVLNGAQNSNAWHGGILPGIPEVIGPNYAVLSLAHFTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNS1</accession>
    <name>A0A5B0MNS1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNS1"/>
    <dbReference type="OrthoDB" id="2510764at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="287" mass="31656" checksum="BBC4834BF678405A" modified="2019-11-13" version="1">MLTYCDPKTSHQRQVTLETGADNWGTDYYTSDHSSHLAKAHQSGPAPSTTAPQINHSMSTPANKSVYTTTAAEQASATKPIGAAHPLKASKKPVARVPKKAKKPISPEIVPSEWDTSSEEDTKSDKPKPKSSAAPELKKSSTTSDPFKSKKPIDKLIDKDILKNITIKKSTPAEKTSSLASAPSVPTDQTHASFTTEPYEPADSSDLDVIAGATAQLWSKPKTSKAPLAEDILQRYHPLFHPLLQAVKLQQEYYRRALETNDEDPPLGKLLPPLHLRYPNYFWFWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP93</accession>
    <name>A0A5B0MP93_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_029826</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MP93"/>
    <dbReference type="OrthoDB" id="2503237at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF39">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022836736" description="FAD-binding PCMH-type domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="7">
        <location>
            <begin position="82"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1078163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="380" mass="41002" checksum="D17B0E514CE9DE0E" modified="2019-11-13" version="1">MIYLNILSFFGYLAGSLSATRSTTLLDSCSIAERGHTTAKRHSSFQDCLRTEIGASDHISSRIVFPGDPSYPLSTLTYNLAIQHNSSAIVYVSSASHVSEAIKCASEYKVPVVARNGGHSYASFSSSPHGLIIDVTNLKDFSFATNHVGEVGEMVTFGAGLRLGDLDMALQEHERAVPHGVSPYIGVSGHATCGGFGTASRMWGLFSDHIVELEVVSANGTILKVSDKTNPELFFAMRGAGPSFGIITSLTLRTHEAPSNVILFEIDYVFPNPDSAAATLSHFQSWGLKSAPSQLGIRWSVKLNHNANSSKKIDLNWRLQGSFLGHMARFNRTIGRLTSGFSVKGQVVRVERFDWLESVRALGGSDALSSEGINPSNDNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQI9</accession>
    <name>A0A5B0MQI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQI9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023066865" evidence="1">
        <location>
            <begin position="23"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="196" mass="22501" checksum="F2130337E39ECB7A" modified="2019-11-13" version="1">MLISAIHIQFLLVGLLTVSVTPSSIWEIGHPIEADGCETSGGPLHGLENGESHVPQQHGEYTNSVFELPRKFIYACISFLPSFFGDKKQSRSISMDPEVKLKFREQMKHYQKHREELMKFDLKLRQELNKPLDIFNKENTSDQIKILEDSRQDILALGKQRIHQLIKGDPANLRVLRFLCPNTKFSAYEPLIPEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQQ2</accession>
    <name>A0A5B0MQQ2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQQ2"/>
    <dbReference type="OrthoDB" id="2506999at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="59362" checksum="9F6092616F04CF03" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRH5</accession>
    <name>A0A5B0MRH5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000477</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000138">
        <property type="protein sequence ID" value="KAA1078708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRH5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="131" mass="14729" checksum="804046FBCD638329" modified="2019-11-13" version="1">MHRETDGGDGEVLLQMIENLRLRLILMWMFLPEYLDITASLRLILESIVPEIPTTTASAAELAAYSRNSHPHRATITASSRAICIRTTKHAEAEETLPNHSNSAFIRTVMQRRKEGMQSNAHLIPHESPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTA8</accession>
    <name>A0A5B0MTA8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA 4-demethylwyosine synthase (AdoMet-dependent)</fullName>
            <ecNumber evidence="4">4.1.3.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_025299</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TYW1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTA8"/>
    <dbReference type="OrthoDB" id="271553at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="14">
        <location>
            <begin position="26"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="15">
        <location>
            <begin position="344"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="201"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="689"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1079822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="715" mass="81119" checksum="5A4EDD57EC9AF489" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXN1</accession>
    <name>A0A5B0MXN1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000448</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000129">
        <property type="protein sequence ID" value="KAA1081725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXN1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1081725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="10908" checksum="BC83BDE88313F672" modified="2019-11-13" version="1">MWIHQSPSVPSRTAELQAGNTRVAILSSALKGEEEYGAVPSHLIAFATAKLRGVSARARADSMHFGSVSRSSRGAALLCFSEASERCAAAVLLLAFFFSKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXU2</accession>
    <name>A0A5B0MXU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029670</name>
        <name evidence="3" type="ORF">PGTUg99_029058</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXU2"/>
    <dbReference type="OrthoDB" id="8249012at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21521">
        <property type="entry name" value="AMUN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21521:SF0">
        <property type="entry name" value="AMUN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="261" mass="29693" checksum="D7673A298DF68B6F" modified="2019-11-13" version="1">MNPRQASLRSSNRASGSTSKRSLPDDTGDPKRKSQPRKRSKKPAQAQASHTSEPDIFPNYEVLWKSSEASAYHRALLLKQSQKRELNAKERLLRVLRWKLLRGRFRATLPALVSQNSTEDVERVVKNALEQLSPCQTIDQVLHSGALTTMCELRGIGPATAAAFLSFEAPSLIAVFSDEAASFFENRLGLIKYTLPFYTSFVECMQAKLQELAKLDDRWDLRRLERALWTHQVLRKLLTDQEWTQFIHISDLQPEPSNTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0E0</accession>
    <name>A0A5B0N0E0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">UNC-50-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_031756</name>
        <name evidence="9" type="ORF">PGTUg99_026875</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0E0"/>
    <dbReference type="OrthoDB" id="10027013at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007881">
        <property type="entry name" value="UNC-50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841:SF6">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05216">
        <property type="entry name" value="UNC-50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1066499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1081894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="35730" checksum="0A1B0EE7465B91E4" modified="2019-11-13" version="1">MSKHLYALPTAATASGSSSTNHGHGGGQTSNPAHSISRSTSPSRHIPASASLLPSGPFARPRSGPDKLKKLLSRLLKPSRWPTMDFELAAWQMSYLCIAPRRVYRNVYYHKQTKNTWARDDPAILILLIGSMAFVGFLWNALYLRSWSPISWLGLVSRFILRDFFLSGLIISTLLWALSNHFFTHSSHTHATDQRVEWQYAFDIHTNSFFPLFLNLYVLQLILAPLVLRHNWVSLWIGNSLYLIAVTQYSYVTYLGYNSLPFLIKSEILLIPILAYTVLYFISLLGFNLASHVLTYYFSSFFFPPSPIPPPTTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0K3</accession>
    <name>A0A5B0N0K3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014194</name>
        <name evidence="3" type="ORF">PGTUg99_018848</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000408">
        <property type="protein sequence ID" value="KAA1088014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0K3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1088014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="85" mass="9285" checksum="2A0DC98B9D669017" modified="2019-11-13" version="1">MFSFFHSSSSSHPINEALAKIKTHSGPPPLHSVPRPFGARRSTQIVGRLELEPAPLLEADNEEDDTSDEDIETNQTDTGTKLGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N174</accession>
    <name>A0A5B0N174_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N174"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022750307" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="18872" checksum="AC8B2E619C17CC95" modified="2019-11-13" version="1">MDACKFFLILISLLAHSFLVSSQASCTKKYHLNLADCTTARNSIKFVNGDNGKGVVSQGELEIAVGRGTCMIHILNFVEGVKQPDILNAIDEILKTCAPAYDGTSQPPTFVARVTKNLIGTDPINQPKCSSTTPSVIKTEDCKVAIDRIGLDKSTGLLTQDKNFGINSVYQTCQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1R5</accession>
    <name>A0A5B0N1R5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026941</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1R5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="14211" checksum="C3DB954313066B54" modified="2019-11-13" version="1">MGRIGGCRPMDRATGWAKLSCLKWLGLEPVDLTEIGSLLVQQSLSVDQLARLRSYLKDNSRKTIRKIVEQLTLALYRGDTHHPNQGIQCKVTAGSHGSNALFLEETTSTSPDRQTTVENGCASAQFDNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4G5</accession>
    <name>A0A5B0N4G5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018346</name>
        <name evidence="4" type="ORF">PGTUg99_025578</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4G5"/>
    <dbReference type="OrthoDB" id="416093at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF589">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE [ACETYL-TRANSFERRING]]-PHOSPHATASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="137"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1093361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="478" mass="53098" checksum="CDCFBC31D2E9CA73" modified="2019-11-13" version="1">MLRIHPTRIRTNYHHQLTLSRTRRIQNNNHSTTTTTTTTTTTAPPSSAYEARLTQTYEIPRIGKIKVSLQSESVLGIAQSRGERPYQEDFFALASLNLDALELDKTLRRAQDYQRLIANNQAKYRKRTPNNTDQPSSSHIAQNKDHKQTLLQTLFVGVIDGHGGSDSAEYLANNLARIIEQSKLSDIPKVIRKYRSIGGYFRRFRGGFLEEIAQEVYNNNNTDPSRKGEGDDKDQPRMGVDERLSLSFLLADQQLISRCPKSGAVTTMALITPLPIEDAEQSRRIDPHFSSPLLSLVLAHLGDTSALLCSATNGKVLPLTEHHHPDSRVESDRLRRIGTGLITDSFGESRWGGSLANSRGLGDAGFKALGVTGEPDIINKIINGLDWEFLVLVSDGISNVISNQEIIDLCKRTKSPTEAAKRVVGFAESLGGRDNMTAIVVPLLGWANPQSVDLTLERREFRLKQFSSSVGSGRQNRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7C0</accession>
    <name>A0A5B0N7C0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zona occludens toxin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015066</name>
        <name evidence="2" type="ORF">PGTUg99_007519</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7C0"/>
    <dbReference type="OrthoDB" id="2316594at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="52062" checksum="FFD69C26BC79047B" modified="2019-11-13" version="1">MSSGSVRNFKPPVLNDPEVDLLQVQRSDGTSNLDSTPEHSTRNAVLVTGEAEKSSAGQFPQRAVVGSIVGQYAHGFPRITPNPKLYLNVNTPFSTLICGVQGSGKSHSASVILEGCLIVDKRIGTLPKPLAGLCLHYDRGSINYPCESAYLGLPATRAQLGLKSTSSVPVTVLVSPTCLETMRNIYKPLKGVNVMPFYLSTSNLNSSRMLTLMGFDESAIMPLYLQRAMTILRSMGADRFNYNAFKNKMSKEPLNPGQKSSYKMRIEMLDSYLTGKKSSDVSSYFKPGHLIIVDLRDPFTNSSLVIALFEIIVGLFVERHMETGKVLLLDEAHKYLNSDQCSARFTESMTSLIRQQRHFGMRTIISTQEPTVVPDAILDLVSFLVLHRFSSPTWIKHLSKHISVDQDHETDSDWSRMISRLALGEAIVVAPTALSIKSDSISSEPLATGFFITRTRKRLTYDGGASIVAYKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7S0</accession>
    <name>A0A5B0N7S0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003033</name>
        <name evidence="3" type="ORF">PGTUg99_017788</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000145">
        <property type="protein sequence ID" value="KAA1128062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7S0"/>
    <dbReference type="OrthoDB" id="2510228at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="367" mass="39468" checksum="49134DBB00C40F09" modified="2019-11-13" version="1">MNLIRVSRPTTLACTSLILISSQMKSFHALPMFTPNAHELIPGHGVVPMGFIHNPPSEPGLPLGTVLGVPAIPPPASPVIPTTPVTVSHIISQDGAHVYLTSPVIASPGVGLAHLPPAHPMTLPLQSYPHPATIALQPLPHPEAMTLPTYQPAMSFLPHPSAGVPFQPWGSTGVPHHWTEQFASGGCLGTPTDSSWSRRFPGTRRPLSGFNKQDKVKKAGKEESQGENITNLDRSSPSETSPQKAETPSTDEIPSSGTSAQEKNPVEVSPPETSPIVRSSASKKTIKRAGRRFRNIRARKLLANSNEHERSRSEYSSHEQGNKSLDHTGSSTSQSKEETAVKEELTRDLIPGAIPENDNRVVKNETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NA64</accession>
    <name>A0A5B0NA64_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003991</name>
        <name evidence="2" type="ORF">PGT21_004328</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NA64"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="151" mass="17426" checksum="BE7508B8EB4DF8C4" modified="2019-11-13" version="1">MVAKPKRRLASGEVDVNTSPSSKLASPREQRKQPKRRTLHSQLHSSSPPSPQALIDDPEEQEEKHHQQEPQPSSSSPSHNQSISDQRPSLTTHQQAQEENDKDQEEEHQKTIKEREARERIFDEFKDEYFDIVSPNSRPHSKNVNESCMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NE31</accession>
    <name>A0A5B0NE31_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019722</name>
        <name evidence="3" type="ORF">PGT21_020005</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NE31"/>
    <dbReference type="OrthoDB" id="2514522at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="144" mass="15541" checksum="219A27F617B03504" modified="2019-11-13" version="1">MVDELLTSPDIARLVNAQAGQPRLTASLVHGQDRNCPPGKSGPSEETFVEAANKERARKKVALEAASTGHSGTLESSSQRSYSLNADLPRPPRHVLSSQTLVQPIQNPSSQLALSDLPASITTTTTTNNYNPRSTLYLHHCRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFL3</accession>
    <name>A0A5B0NFL3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFL3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1087322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11653" checksum="5D05C441A5B34381" modified="2019-11-13" version="1">MANYQVTTISQHQSVVPLAEVTYRLPADTHFLAEFQQVIRTVPDSNPIHDQLEDKSSDEKKLVKPFLSRLEVSPRHSVVSSSSSVEQDHFEVQIMKPIIIIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI35</accession>
    <name>A0A5B0NI35_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI35"/>
    <dbReference type="OrthoDB" id="3267335at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="155" mass="16751" checksum="31B03418A498AFEA" modified="2019-11-13" version="1">MRLSFQGTLSPRRQKTTLGHARTQVLGTIFLATTLVFLANLTICQRSGGQFFTPGLLIVNGPAPDSKKPAGKKILISLEITGDGVLKPSNTDAGSQLATAILSVEIYLANRERNITISQGPELMQGERGSSVKHMDFVIPEVIHVFLLPGHLQFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NP76</accession>
    <name>A0A5B0NP76_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000995</name>
        <name evidence="3" type="ORF">PGTUg99_003947</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000388">
        <property type="protein sequence ID" value="KAA1090346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NP76"/>
    <dbReference type="OrthoDB" id="2499475at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7872" evidence="1">
        <location>
            <begin position="228"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="452" mass="51050" checksum="AF895E27AC4F565E" modified="2019-11-13" version="1">MKAVSFKAYSWSIALYWGVVLNSIFFSTADDVLHDESENRKATSRANNQTGIDLHDSDVRDACQPRTLSPALWSKLNLNTYLLQYPGGQETSLEDLAVKSKLLNFDCGIDKLCYAGQLCSPVKGKDWYVLVAAQEWNSYMNVVYQAVGFAMTMMQGIIPSMIKDFFPDEHDDWAIAKAYLTFFSSCAKVHPTEGHISNTKWWMQLVQGQIGLTAGEANIMDYAVIPDPVNQHDKWTYFIYQLSKTQDLIQSKLTQNSEEIISSGISTDQGIYGVLKDGAFLIDHVHCQSLVNLAAGQQSEMKLSIQLNLLAAIWEKQKFFILRGSDPCDGDGENGASSEKNQLSYCNADNIMMKIVQLKKKNRLKTEIYNGNLVFEKYNFTAKFLTERAWKCQSNTKQFQPEVWNLSKPIKIDSECSFNLAVCDLTAPDTRELRKQGKSITKICRNYLGLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPK7</accession>
    <name>A0A5B0NPK7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinetochore protein Spc24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_028429</name>
        <name evidence="13" type="ORF">PGTUg99_022618</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="13 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Centromere</location>
            <location evidence="10">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SPC24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPK7"/>
    <dbReference type="OrthoDB" id="2500316at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013252">
        <property type="entry name" value="Ndc80_Spc24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08286">
        <property type="entry name" value="Spc24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 10" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1075852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1091205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="251" mass="28674" checksum="9D757C7831C3A503" modified="2019-11-13" version="1">MSQLRSSWIPSSQPESHSQMRGTNTTENEAGERSMDQHQTAADEPPSQSDIWMTTLVLAQDMAVVLKEGVEEEVGMMYEALDQTFKWNERRRERVENAHNELLRVHRQVEAKKDALEKMKANSTARENQAEIYSLDQSAYEEARKIKGLDYSIGSVKARTTKLQTELDELEQSEPTRSPDFVIESSVLKTKLYHDFGFVQVRTPLQEGNNSDPEKLFIRCDSDCTASRVVQKPKTNRDGFTLANEIWDAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUZ2</accession>
    <name>A0A5B0NUZ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUZ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="85" mass="9507" checksum="B4112347D3CE13BB" modified="2019-11-13" version="1">MYPQSVNAELEQLSTGGGPSGPRKITIRYAQALSLQPTPSSSHGLWLPHQPGRPLPTLRRLPTGGNRRRITLHQQPFRRVVVFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NV47</accession>
    <name>A0A5B0NV47_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_009966</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NV47"/>
    <dbReference type="OrthoDB" id="248747at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Longin" evidence="6">
        <location>
            <begin position="34"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="7">
        <location>
            <begin position="167"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1092652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25102" checksum="4DE3A0C446C0AC68" modified="2019-11-13" version="1">MSEIVPSISLSQDQSKTIQSTTPLPFQINGHPRRTRRFRHQKKILTEHTVDTSKNFSNASSAILAKIPPNNSRLTYAAEDYLFHYVKPHDVVFMCMSEESLGRKIPFTFLNDLQNKFFNQFSQSDLASTAPYGLASSITELVTLPTGHNPQAADLNQPQQASSSNHQINLVRLELASVKGIMIKNVGEILNKGERIKLLLNKTDNLSAFRKRSPLATQVVVGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYE2</accession>
    <name>A0A5B0NYE2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016724</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NYE2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1092869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="11155" checksum="AEB1018135876042" modified="2019-11-13" version="1">MKSSDQFNKRLEMSIRDDQLPRFVSKYSSDCIVFVIQGRKYACLGSAAGIPDFEYNVFSRDRHSIPIQDIGVYQIQIPPNTDTRLVLVAALPESPNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P017</accession>
    <name>A0A5B0P017_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P017"/>
    <dbReference type="OrthoDB" id="2495279at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="2">
        <location>
            <begin position="228"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="512" mass="59110" checksum="397620051162EA50" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3B3</accession>
    <name>A0A5B0P3B3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3B3"/>
    <dbReference type="OrthoDB" id="96345at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="12744" checksum="8324AC2ED56F494C" modified="2019-11-13" version="1">MYGVQHNNDLILSNDSLKPLTPYMPPGINHRIQLLRRSYNTARDFIAHAQREPNIHDGIILLYARRVCPHWDLLHPIMGPQTADTHDEQPENHNHEDATSEVSEDESTNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P533</accession>
    <name>A0A5B0P533_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P533"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="160" mass="17811" checksum="E4798EDD3FFF933A" modified="2019-11-13" version="1">MSSPNTPPTPQSISSPSTAEEAIPLPEIRELHQETILETNYMLVEPPSDFHYNNTRATSPVLVERATVYPTPENEEPDIRRRVEDYSHEQVRERDLAVIMTPAEPEPCGRFGFNRASYGYYSPSAISFSDLPGQGTADSPYYVNTPPPGAEEDADRGKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P596</accession>
    <name>A0A5B0P596_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P596"/>
    <dbReference type="OrthoDB" id="2509300at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="307" mass="34650" checksum="51D6AC0CB763C285" modified="2019-11-13" version="1">MDQIIYGNNDDSTIKFPQGYGQSSRSSSQPEGNTHDSEEDLEVANHRQLFGDAPVDDVTMTNRPDLVLPEHDQTDRVCADLQKKLALDANHLKIALLTSKCPPEERHANVVFANAAFHQLDAEKHNSPVVHAYNDALKDFVRMKARMFLLIPSLEAYSNNPHKDGTLSKSLYYLTLLLSNVLETQRILIKGDVPDRDNLCQSPTKQQKDVQNTDQDSSYYECSDANSNGLCKASNGLLLCSHAKQAITVGRNRRKIVIPSNFIERFQQAHAHLVFLKDDEMFSHKKRFKLILEESRDALVVPTLHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8D0</accession>
    <name>A0A5B0P8D0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8D0"/>
    <dbReference type="OrthoDB" id="2511478at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="291" mass="31494" checksum="8BC231D7D4C3594E" modified="2019-11-13" version="1">MVDVNATRATSKKIKDSSSSKTRNEENSIANSLIPGGSNVATSDASTQANKGSSNVQALNGSLTNPGDGRAGVLINDGEQANDRMHSPLVDTSEHRRDRAPAALLNPSAKATTSAITDSQAVDEEEEDRSPAIAAPSIMTERQQMWDRAVTAQESGDKDLAEMLFKAIGRIGTNAPRQISDAGIFAKPVMIDSGEITYLGAKEVTATQPATRPHVESQEPEKFFLTEGDLVYAIGSVTNHNSVGFAPFLDENIRKLRSPLPLTIFNRKWQQRAMAHHLDRRQRSDDLSADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8V1</accession>
    <name>A0A5B0P8V1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026410</name>
        <name evidence="2" type="ORF">PGTUg99_009672</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8V1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1131694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="177" mass="20304" checksum="809B06D61443A485" modified="2019-11-13" version="1">MFLRYWTRQTWTTSAVIALIGYLQAFHLTEVQATSLVSRQVKPEDVSYTCAQSFQINHPTRGKTDCQIASGKLMVCNACPPMWQFKFINCRRETYYEDMKPAEGLTVIPTMFTVLQGVSYIAVWGFEVERINHRTIKTTHAYKCYDEPTHHNRARPVCRGCTEATPHNPKGPHAMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9M2</accession>
    <name>A0A5B0P9M2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9M2"/>
    <dbReference type="OrthoDB" id="8026949at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="178" mass="19108" checksum="7EDD849EF575A9F9" modified="2019-11-13" version="1">MSTRQVQNVASLVSTVKSLDGTAAVPKEDSKSDSLVARSTHYLKGYNPKEVIANCDALSELACSTICLTLSQSFWESKHDPNTPIALWIGHVRVAANELLTINELLTDWQVANCLVAGLDSSWSAVKDSIIYTAQDMSLDNTIGALEAHEAGAVSTVKLGGYELGSFDDGDKVDVVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAC1</accession>
    <name>A0A5B0PAC1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">U3 small nucleolar RNA-associated protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">UTP10_1</name>
        <name evidence="12" type="ORF">PGT21_028629</name>
        <name evidence="11" type="ORF">PGTUg99_008608</name>
    </gene>
    <organism evidence="11 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="11 14">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the HEATR1/UTP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000349">
        <property type="protein sequence ID" value="KAA1097228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="31183at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012954">
        <property type="entry name" value="BP28_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056473">
        <property type="entry name" value="HEAT_Utp10/HEAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022125">
        <property type="entry name" value="U3snoRNP10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040191">
        <property type="entry name" value="UTP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457">
        <property type="entry name" value="BAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08146">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23243">
        <property type="entry name" value="HEAT_HEATR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12397">
        <property type="entry name" value="U3snoRNP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01036">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BP28 C-terminal" evidence="10">
        <location>
            <begin position="1838"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1097228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1104634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="2127" mass="234189" checksum="D345521858FE940D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PD42</accession>
    <name>A0A5B0PD42_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_010030</name>
        <name evidence="8" type="ORF">PGTUg99_006447</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000316">
        <property type="protein sequence ID" value="KAA1103779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PD42"/>
    <dbReference type="OrthoDB" id="10267436at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000464">
        <property type="entry name" value="Ribosome assembly protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="228"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="271"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="318"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="442"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="484"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="526"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1099515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1103779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="557" mass="61703" checksum="AB5ECE6433288A7F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFI0</accession>
    <name>A0A5B0PFI0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Capsule-associated protein CAP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CAP10_8</name>
        <name evidence="2" type="ORF">PGT21_006295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFI0"/>
    <dbReference type="OrthoDB" id="202415at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF118">
        <property type="entry name" value="BETA-1,2-XYLOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="1">
        <location>
            <begin position="422"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="782" mass="90407" checksum="B1188D7786348A30" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGC7</accession>
    <name>A0A5B0PGC7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008066</name>
        <name evidence="2" type="ORF">PGTUg99_016747</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000210">
        <property type="protein sequence ID" value="KAA1123258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGC7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1123258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="155" mass="17403" checksum="B40B5DC71D149279" modified="2019-11-13" version="1">MVVVVGAQSSGRNKSGYRLWLSPLIEVSAPRARADAEARNRMGLPRIRISKPERPASFRGLHHNGHHLSMPAQHSYARHPKSQVLGALYASAASCQTSTEDQAFQSIIISPPYREWRDHEGKEIGDSKEKLMTAEVSIEFSHQTYSAQLKDYPHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRI8</accession>
    <name>A0A5B0PRI8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRI8"/>
    <dbReference type="OrthoDB" id="2150628at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051667">
        <property type="entry name" value="Archaeal_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096">
        <property type="entry name" value="YALI0E33429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="49"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="409" mass="46903" checksum="FED8AE54D992342F" modified="2019-11-13" version="1">MFFRPATYSISSQRALCIRAPGVLKGRLGRTYYSRPHEEERLSRRLKSAPQLTLILGPPSSGKTALARQVASQTRPDGTPEFHPLTVDLRAVDRFHDGSFLRAFVRNGIRAAEIDPFWERPFRGFDDLARVLYSKPPQDVPSSLMAAEVFNELAHRLQPLPYYTRESVRALVLIIDEANHFRKMSDKAIMTFLDFAVRMTTKEKKMHIIMTSSDSFFLQWIGKCINPTYLDWFVLGDMTRDEAHRYFLHALANHCRLSEEKKAMLGTVDSDTIYRLTGGRPFFIESYIRQVHQSGFFVDPLRFQPVRQAYACMFNSLGDEPKTYGKAETLAVSSLLVNSPGHHTSYGNLALKLGLPVVEEMFERNFLQYRPPSTFSRDLDPSPHETIVTAQSQPCLRAMEWFVNSHRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRQ7</accession>
    <name>A0A5B0PRQ7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Beta-coat protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SEC26_1</name>
        <name evidence="15" type="ORF">PGT21_017239</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
            <orientation evidence="1 10">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasmic vesicle</location>
            <location evidence="10">COPI-coated vesicle membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRQ7"/>
    <dbReference type="OrthoDB" id="10261439at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000451">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011710">
        <property type="entry name" value="Coatomer_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016460">
        <property type="entry name" value="COPB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029446">
        <property type="entry name" value="COPB1_appendage_platform_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635:SF0">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07718">
        <property type="entry name" value="Coatamer_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14806">
        <property type="entry name" value="Coatomer_b_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005727">
        <property type="entry name" value="Coatomer_beta_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="12">
        <location>
            <begin position="19"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit C-terminal" evidence="13">
        <location>
            <begin position="689"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit appendage platform" evidence="14">
        <location>
            <begin position="830"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1103414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="970" mass="107942" checksum="6033FB6D9FF2F628" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PU48</accession>
    <name>A0A5B0PU48_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PU48"/>
    <dbReference type="OrthoDB" id="10306378at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="485" mass="53278" checksum="EACF66A00A6022B2" modified="2019-11-13" version="1">MQVLRLSSPSLIHPEHGSLFGSPIMSSTKGALSLYLLYGLLRSLGSLSRSLRRKNTTRPRTVLEYKDYYSISSDHSPTQSFDEDGDSYTSFGSIQTPPRLRAMIRSIFDGTLNSPIRSFPDSDYGDADSYHERVTDHSAIVAEGLNEVGFLLGETFGDRRAQLLASAAPPYPIDASASPSSDPDGEYELDDSTAASIPDIDANPQPIVAGSADLNGFCCVPDATSDIDGDVRTPDPNDSAVSHIDPSLRLPSESDDQYDIDSCFSLLDPYEDPTNTTYVPPKKSKQSRVGRKRSSGSLLAKPKAAFVRPRDSRGRFVSLQSRNPGKLKAKDNDDQPLPSSSSSSFLHRVVADSNHPYLAETARNTFLNTVLTSPEEAFPFTFHLPDPFAAHSSLAGPSVLGTHPFLPPTPFINIRPDDPRLQFRTAFVDLLRVIVPFLSNFTLAPGDHESFKLLSHAVVAAQDWYIRSQHLYPDVADHQLQFDQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PX82</accession>
    <name>A0A5B0PX82_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010714</name>
        <name evidence="2" type="ORF">PGTUg99_033326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PX82"/>
    <dbReference type="OrthoDB" id="2504239at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="207" mass="22416" checksum="B9B88B7B641A3A45" modified="2019-11-13" version="1">MENNKTMRSKTSARKDPDVLPGGSALSVVNPPKGAETNPPKGAEINIIAPTSEPPDSDGEGSVNLIAKNPILSIETMKDVITSTSVPPDEDLATERSYVWTKMKEAQAIGDKVLAKILLKAYNDLEPLVVKVPPKLTRSISALPVMTHLSEDTSPSKKIVVKKETELEDNLVYAVGAVTSHQDIGFTPYFDENIKKLKAPLPLTIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PY92</accession>
    <name>A0A5B0PY92_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PY92"/>
    <dbReference type="OrthoDB" id="2506983at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="752" mass="85830" checksum="46BFCDA582C6B327" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZY5</accession>
    <name>A0A5B0PZY5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_035790</name>
        <name evidence="6" type="ORF">PGTUg99_024352</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZY5"/>
    <dbReference type="OrthoDB" id="2436667at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13156">
        <property type="entry name" value="KOW_RPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000014">
        <property type="entry name" value="60S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000915">
        <property type="entry name" value="60S_ribosomal_eL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041997">
        <property type="entry name" value="Ribosomal_eL6_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005568">
        <property type="entry name" value="Ribosomal_uL6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01159">
        <property type="entry name" value="Ribosomal_L6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03868">
        <property type="entry name" value="Ribosomal_L6e_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1106511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1126294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="250" mass="27870" checksum="935357A7F039B3FD" modified="2019-11-13" version="1">MAVTTKKNLPRNRAIGPHVNRLSKSQLYSKKALYKRPHKPIPSTKAPEVDYAATKTVKVGGAKNGGERLVPVHKAPRFYPTEVQSKPKKPRKAHRAPGLRSTLTPGTVVIILAGRFRGKRAVFLKQLESGLLMISGPFKVNGVPLRRVNQAYVIATQTKVDISKIKLDEKFNDAYFAKEKKTRDQRAKAKEFFAENGEREKKKCPDGRIADQKTVDKSLIAAIAQTPVLAKYLATPFGLSNGDFPHRMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1L4</accession>
    <name>A0A5B0Q1L4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2501529at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF159">
        <property type="entry name" value="LD03170P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="892"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="654"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="846"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1139"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1259"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="604"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1155"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1188"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1219"/>
            <end position="1233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1536" mass="169488" checksum="4AD8C0BEE7475E85" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q553</accession>
    <name>A0A5B0Q553_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004675</name>
        <name evidence="3" type="ORF">PGT21_006074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q553"/>
    <dbReference type="OrthoDB" id="2507425at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="153" mass="17343" checksum="1AB1A86426A4661C" modified="2019-11-13" version="1">MIEDLRSYKWPRLKGEGQWIRCFAHILNLIAQAILRPFGSNKRKNTAGASPHENLDSDNDETDVEPDDPDEQIKFFNEGISGDNDNEDDNEETDDDAPLASNLIGDDEVELEDDDLNDLSDEGEEDQYTTQSCKESLSKVSKHLHLMNNLIAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5A5</accession>
    <name>A0A5B0Q5A5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA sliding clamp PCNA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">POL30_2</name>
        <name evidence="12" type="ORF">PGT21_001282</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="7">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processivity during elongation of the leading strand.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5A5"/>
    <dbReference type="OrthoDB" id="534348at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000006">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000008">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.150.10">
        <property type="entry name" value="DNA Polymerase III, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="10">
        <location>
            <begin position="62"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="11">
        <location>
            <begin position="188"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="11">
        <location>
            <begin position="408"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="263"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="318"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1108124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="480" mass="53998" checksum="7083110B24C70899" modified="2019-11-13" version="1">MVSGSRRASLTAGSALGPSPPLERVRLHSQQHPSQGEPTVSWLQDFHPPQPPHLLSYPSAIMFEAKLNQAGLLKSVLDAIRELVTDVNFNCDEDGMKLQAMDNSHVALVALQLRASGFGQYRCDRQMTLGINVPSFQKITKCAAPQDIITLRAFDDGDVLNIVAETLNTDRVAEYEMKMMDIDIEHLGIPETRYDAEVTLPSSEFNRIIRDLKEMGESIRIEATKEGVTFVTNGDIGKGSVTLKHNADEKKTLKVDKDKDVIDIDSDEEKDEEQNDDEGSDGDAEERKLKKALFKKDPDEDPSQNATQETDDEMDESQDVKPKTNGAAKEEKPKLKKKKIVDDDDEEEEADEQQKDVENDEEADEDEEDEEDQLPKSKKRKLSKKAAEKSKKNGKSKAKERKSAKEKKQEEEEEDLSVSISLQQSVSLTFSIKYLSNFTKATPLAKRLTLHMSNEVPLLVAYEFDTGYVRYYLAPKIEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q602</accession>
    <name>A0A5B0Q602_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020551</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q602"/>
    <dbReference type="OrthoDB" id="10383712at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="156" mass="16972" checksum="AB27492289DFB2FF" modified="2019-11-13" version="1">MLYDSQPTLAHHQPAPSLGIRVSSEVSAVQHPCSPAIKAMGAMPDPTNHGGDAGRGPRLVHKCQDDDQSLAEASAANLDCFKFGARNESIILPPEYLSDYKLLRQNGNSGPLVDVSLLCSVQEALTSHLNKAIHTSNEQMVWSWPFECEFSLICDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6F9</accession>
    <name>A0A5B0Q6F9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6F9"/>
    <dbReference type="OrthoDB" id="10294978at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="237" mass="28442" checksum="DB8BDB87E659FF36" modified="2019-11-13" version="1">MQNQRFFYQMVDKDAAADLEDVKQTVQKAKQQISDVGLQRHYQEDMNQLDRRVTMIDYLLHEKYDPHSVSKDLENKLFEEDRRLIAEGSWGTFEEHGAKVLTDLKQLLFLNPLSKLVSTDRRIHQFRKSIFFAIRLMIKHDLVENVQEHAIFKDRRILQQLLRCHLVENEWKPSSELPAYGTFLDGDKFWSLFFQQYSEKVLQEAEEKEKNRHLVRIQEDVKPKYMRFVSDYSKSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9M5</accession>
    <name>A0A5B0Q9M5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000987</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9M5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1109867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="64" mass="7414" checksum="75B78780FA05E721" modified="2019-11-13" version="1">MRNHYLGHHFGLRCLVLSLNNQLDYAGIDRLNNRLDYARIDLGLNYFDLEVDQHSGGDVVIELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QD19</accession>
    <name>A0A5B0QD19_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QD19"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="150" mass="17321" checksum="953FC5FA54098E29" modified="2019-11-13" version="1">MHDPRTLGNSPPKRRFTSWKNSPVKHLQSSPPPSQNRSNTDHIEQAIIWKTSCSSSEPTVRHLPHPLILKIQHACLKENATTTRIQEERAGQSPRSYWIVGIPLIWFLIHNNNNNNLDKCLYRLGRKLRLMPEVSPRRLKNLRPNVRSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QD68</accession>
    <name>A0A5B0QD68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QD68"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="8823" checksum="85CD95D12F890754" modified="2019-11-13" version="1">MEGKSELERYKELLKRVDMLEKKEESKIKELDDADDEELEEKINQWHRDFNAERDEIRRLMKEYSESSSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDV3</accession>
    <name>A0A5B0QDV3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036798</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QDV3"/>
    <dbReference type="OrthoDB" id="10345306at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1111123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="359" mass="37755" checksum="709B7CF4F8BD2C44" modified="2019-11-13" version="1">MLQKYQCILLVTLINVFTLASFTWAFPANRAPSFPLANGRRSVIVTNGPVIDVENRRVRVRRKLAMIMKREGGAAMRTNEMITRRRIIDKRSPKGGGGGTPPKTGGSTPKQGGGTAAKVGGNNNPVKVQQKSTQNQATKQNAGVKKLQNTGPNKLQNTGVNNKLQNTGANKLQNTGANKLQKTDVNKLQNTGANKSPNTKTDTTALLAAGGGGTKAAGGGGGSRGTGQAAQAQAGGKRGAADLKLQVAEVSNGLASINGKKPNQIVKEVDRILSIEKAEDTARGAIKSAKPNDREVTSALATVQKNGPNLIKGLEKLKDVANDPKSTRKAIDDQLAEVNKLRKPLLGANTELIKEGQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QER9</accession>
    <name>A0A5B0QER9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_022064</name>
        <name evidence="7" type="ORF">PGTUg99_006057</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000283">
        <property type="protein sequence ID" value="KAA1111433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QER9"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000252">
        <property type="entry name" value="Related to calmodulin-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF26">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="5">
        <location>
            <begin position="348"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="70"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="584"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="641"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="702"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="763"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="920"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="80"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="657"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="705"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="771"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="797"/>
            <end position="811"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="846"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="961"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="984"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1066121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1111433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1026" mass="112146" checksum="0DB4324F8D3B2C09" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEW6</accession>
    <name>A0A5B0QEW6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
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        <location>
            <begin position="200"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
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            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="230" mass="24645" checksum="4B9B1AB4F988A65C" modified="2019-11-13" version="1">MGSTTKRPKPPGSRTSSVSSIPAAIRNKKNKTVIKVDDGSESGDSQEVSNSETPASTQNSKEASRSDEQELTDHGLNPKEDPTNLGTALELIRDTCSRTTIINSNIAPPTTASPSSASAGSLEDILDPKPYSDLVPQAQASLQLSQAQQQAFVELLSDHRLWTAYLQALIQQSDQSIGQLNQTIQLKSQRRQSILDQLKPGIRPSSDQQATTSPPIQSARPVTSGGLCWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QH72</accession>
    <name>A0A5B0QH72_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_002078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1112560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
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            <begin position="96"/>
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            <begin position="265"/>
            <end position="290"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="433" mass="47983" checksum="713B3A7216398621" modified="2019-11-13" version="1">MANHTMTIPRISDMLKKAPEGLNPYSFAIMALENLEKPPSGPIFRSVLTIFACISAAIMLCCLGILVVPFFQGEVGGSQHNWIVKRKYSGSRANPYFILNNGLVIGLSYLFGGILFQCYVALQFNTMDSNRQTSQFSWIEILKLPGSCATFIQAFTMLFISTSNPSQGGTKRYSIPPGIFNFLLIGFPALTAVASIFLIVHKASLVQKQDVSYNVLINLLTDAAKLWDGGEKILSESRNFLLQATYEVFSLDTFLKNRSRIRIGLFWAFMAIPSILFYVGGVIALVTTVYQPIKTPKKLQPANLNPNKIKTVGNEMENGPIILGKEGSRKLSRSLKFLWLHYFSMSMALSMDAATSMVFYLNKDNLKDIRLARKFFVTNLSSSIFTLVAMIILLARIIGDGKEAKNSASNCTSGFTESNYSASKVAPGNKQYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJW4</accession>
    <name>A0A5B0QJW4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine/threonine-protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ATG1_4</name>
        <name evidence="3" type="ORF">PGT21_031753</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJW4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022708">
        <property type="entry name" value="Atg1-like_tMIT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12063">
        <property type="entry name" value="ATG1-like_MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine/threonine-protein kinase Atg1-like tMIT" evidence="2">
        <location>
            <begin position="270"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="424" mass="44245" checksum="2B3291B1A8C43EAC" modified="2019-11-13" version="1">MATKHQPHHLAVPDYATLRPSSAQRDPDCFKTEEDEDWDLGTEYVVVEKGSVEINAMVDGLSSSPQKPMSLGRRMSRGFMAAKPTLTGLSSSPHRTTSSGSPPHPSLATHSHSSPVSSFPPRPHPMTASPVPTGSHPMNIANFGCSSPSSQGHYGPHPSSPRSFDSGGGGIGSLPLVGKYFPQAVLGTSSPSGPAAPSHTSVGPSGSQAQRLPFTFPSSALCRAILSAGPLQINHQTIPIQSIGGPLTSQPSPHSSASRNGHLDPVESQLLAELEEFACKALVIIHFANQKLAAILPPPPSAFGPSTSSTSNPATFSGPVGAFITSSPSSYHSFDPSMSSSSCYDLHHSSTLDTPSSAPSKPPSGQLAPAAGQALLLYIKALAFLNKSICHAVWLNGRADHPNRLDLRAGPGRATLTCCEESEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQA8</accession>
    <name>A0A5B0QQA8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_035283</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQA8"/>
    <dbReference type="OrthoDB" id="436852at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037869">
        <property type="entry name" value="Spp1/CFP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174:SF1">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="84"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="276"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1115329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="461" mass="51840" checksum="4A10331D177EFBDA" modified="2019-11-13" version="1">MVRRGRGTKRPLPAPTDYHSSHGTTIIRGTSTRPLISYQANQPAVILADVAEPISEAESPPQESNVKEDEKSESDRKSEMEDDRLYCVCRTLYDGRTMIACDRCDNWYHNDCVGIEEDSVELVDIFICPSCQTGADKQTTWKKRCARLGCHLAARVLSKYCSDWCGIERAANRLNNATTDYQRFWNSVRAVTKPEGVVIIECEEPAESSSSQANGIILDDILLNQRTERLEELLAKKLAVERQVQLALSRSKYLGYAISRWENMCLATARALQIQEDPSPDQSTTAAPATTKPKRGGGGRGRGKARGGVKKSETSFLSSATSSTEAPCGFDVRLIWDDKDWAQWTSSEGFLALFRSPESENQEPIQENVNTERVDSNPEATVDYGIEEGVICLIMRKKCDRHLGWQKTRENDFQTEVNTLNMVLDKVSTQIQRLKEEIEERKELWKFRNAKQASKGSQPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQB5</accession>
    <name>A0A5B0QQB5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_036343</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQB5"/>
    <dbReference type="OrthoDB" id="2919105at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF15">
        <property type="entry name" value="NAD-DEPENDENT HISTONE DEACETYLASE HST4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="7">
        <location>
            <begin position="29"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="160"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="407"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="485"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="598"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="485"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="501"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="606"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="623"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1115432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="638" mass="68609" checksum="BF591E8B9AC2248A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSQ2</accession>
    <name>A0A5B0QSQ2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MHYT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_050136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSQ2"/>
    <dbReference type="OrthoDB" id="264015at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005330">
        <property type="entry name" value="MHYT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35152">
        <property type="entry name" value="DOMAIN SIGNALLING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G11310)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35152:SF1">
        <property type="entry name" value="DOMAIN SIGNALLING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G11310)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03707">
        <property type="entry name" value="MHYT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50924">
        <property type="entry name" value="MHYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="MHYT" evidence="2">
        <location>
            <begin position="30"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="994" mass="109121" checksum="FA65A7BC51A6CD5E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTP3</accession>
    <name>A0A5B0QTP3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTP3"/>
    <dbReference type="OrthoDB" id="2507352at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="327" mass="37746" checksum="4686C5B132CC5557" modified="2019-11-13" version="1">MVELQAPLPPAPPKPSFANRIKIKLEYNDLKSQFYQVTNLKESLFHDLSEKQAKEFKLRMECDLILDQIELIRSRMPISEQPEGRAEGGDDWELRRVRKYQENPGGGFMYESDEDLIRGRSSASSASPSSSSSSSAPHEQEQQEQQLQHGQEHREARPAREGREEDEDEDAEAEMDDEEHDPDLDHPENTLMYDHKPPQSVQRHDFASRPSAPPLSSSSAAHQQPHQPHQHHHHQQQQQHHHQPFYQEGVLHQPQDRHDDDDDDAEEEEQRDEEMDDAVGEDDEELVSDSDHHHHPHPHQASFQNNPRPAYNNTLHPHPSSSSSYHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QY61</accession>
    <name>A0A5B0QY61_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TATA-box-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SPT15_2</name>
        <name evidence="7" type="ORF">PGT21_024867</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QY61"/>
    <dbReference type="OrthoDB" id="2127950at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04516">
        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000001">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000002">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1117835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="236" mass="26133" checksum="BF0A52973BD9D37E" modified="2019-11-13" version="1">MAQSTFVLPGPPASASLHNNAGSISPDQLTLSQPEGQEATQTSHHPDDPKGIITNVQGIVPTLQNIVATVNLECRLDLKTIALHARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVVTGAKSEDDSRLAARKYARIVQKLGFEAKFTEFKIQNIVGSCDVRFPIRLEGLAYNHGHFSSYEPELFPGLIYRMVKPKVVLLIFVSGKIVLTGAKVREEIYQAFNNIYPVLSEFRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYN1</accession>
    <name>A0A5B0QYN1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lysyl-tRNA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">KRS1_3</name>
        <name evidence="3" type="ORF">PGT21_025765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYN1"/>
    <dbReference type="OrthoDB" id="21243at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF9">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="89" mass="10244" checksum="67A7E80D7939A578" modified="2019-11-13" version="1">MICVVKHNVDCPAPQTNSRLLDKLVGEYIKNQCVTPSFIFGHPQLLLQPKKSAYTELNDPFDQRERFEEQTQQKAAGYEEAQGIDETFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R058</accession>
    <name>A0A5B0R058_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R058"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="850"/>
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        </location>
    </feature>
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            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
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            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="275"/>
        </location>
    </feature>
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            <end position="902"/>
        </location>
    </feature>
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        </location>
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        <location>
            <begin position="958"/>
            <end position="971"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1006" mass="109840" checksum="95F2C1BC42393E60" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R090</accession>
    <name>A0A5B0R090_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed DNA polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_011628</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="342531at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="DNA_pol_V/MYBBP1A"/>
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        <property type="entry name" value="MYB-BINDING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04931">
        <property type="entry name" value="DNA_pol_phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
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        </location>
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        <location>
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            <end position="770"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
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            <begin position="1216"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1232"/>
            <end position="1241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1118967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1248" mass="138083" checksum="D4F534CF60C6D161" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0E3</accession>
    <name>A0A5B0R0E3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0E3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022734808" evidence="1">
        <location>
            <begin position="17"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="138" mass="15315" checksum="B7A944BF1448EB8C" modified="2019-11-13" version="1">MTSVTFRLSILTVVRTATWFLPKQQKRFDTGVRPGLLFFPRNLLVRLGLSTVGSTSLYCSTHFSACDKDGRIDITVLQTARQVLPATVSQTAACSNFYNSLFKDRRAQSGDCTFGSTVFCQLRRPAHQVLLASDSDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R190</accession>
    <name>A0A5B0R190_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Coronin-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CORO7_1</name>
        <name evidence="9" type="ORF">PGT21_007235</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat coronin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R190"/>
    <dbReference type="OrthoDB" id="347435at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001012">
        <property type="entry name" value="Actin cross-linking, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000774">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF20">
        <property type="entry name" value="CORONIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="8">
        <location>
            <begin position="503"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="402"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="970"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="410"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="423"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="980"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="996"/>
            <end position="1005"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1118825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1020" mass="111801" checksum="6137B0A01E57B856" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1R2</accession>
    <name>A0A5B0R1R2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1R2"/>
    <dbReference type="OrthoDB" id="2506990at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="22726" checksum="2A2D3DDEF04C58E5" modified="2019-11-13" version="1">MPPRTQLKTHATHNQDRPLDTPSPPDQPHDDNDKDVGRNSSEEPSSPRQSNHPLTDAEELDRARNVAANAVSSSYNNYHVPELSDQKDKSGRFMIAYHCKMCSTKINRPMSDSSCGNLNKHAALCLRKQQEASNSGGPLRYATLLAQKAAFSGKNGQKPLKTAVAQRSGPFFVAHTRSVSGVVAEFRYATLIANSDSAALRYTNRYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3E4</accession>
    <name>A0A5B0R3E4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_037089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3E4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="219" mass="24819" checksum="429E8B6D2B85A408" modified="2019-11-13" version="1">MLNINDLIHGDPDDPATRLEGAEESHYDTSSDDHDSPPSNHMEITDPNNMFASLPSYDVNMTDRSSAPGAEKNVEQICSDLQSKLMLDPEHLEIALIRSKASPEARHASLVFTNGAYHQLGNKGTQKPSFVYDDTFRDFVRTSARMFLLKPTLEAYSNNPHQNTDIPKTLFYLTLPDAWKDNHLPPRGVWANVEVLNHYRKVVGELLKYQQSNLRTLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R446</accession>
    <name>A0A5B0R446_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U2 small nuclear ribonucleoprotein A'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LEA1_2</name>
        <name evidence="8" type="ORF">PGT21_032840</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R446"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000026">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="186"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="244"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1119734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="274" mass="30572" checksum="F3E33FDC7E6CE506" modified="2019-11-13" version="1">MPRMSPELLLSSHSYLNPLADRELDLRGYQIPAIENLGVTKDSLDTIDLTDNSIRSLINFPKMSRLKHLYLSNNPLSFVSPKLPEALPNLSSLVLSNCKLNNFSQLVGILKAFTRLEFLVLADNPVAAHKLYRDWCIFNCLKLRVLDFKRVKDKERKTSRELFYDPITKGPTGLAAALSIVESVEGETTTGGEEESTIQLGSKPKIEPGQAGRTLTAPERERIKRAIEQATSVEEVRRLKRMLEQGFIPRDDPKNLSPSVAPPPPTSTPSVIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R5K3</accession>
    <name>A0A5B0R5K3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_037306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R5K3"/>
    <dbReference type="OrthoDB" id="5390at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="187" mass="21102" checksum="A0D57761D98E59AB" modified="2019-11-13" version="1">MASHQPVFFPFTEADDDGRLLDNTYSDGWMASYRNRRRRCRPELKRVKEHCDRWMEDWEAGRTPKLPQDEREPEPSNGMMGGHDGQEAEDGGGWTVVSRGGKHGRSATTTATTTTISSQPQQKARGADPYAQSSMADRDASAAVKVMKRKFAKTQAINNLKSNDEEPSQNSFYSTKPADTRKRKKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R7G4</accession>
    <name>A0A5B0R7G4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030646</name>
        <name evidence="3" type="ORF">PGTUg99_031110</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R7G4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="188" mass="21316" checksum="A729EDF6FFBB91E2" modified="2019-11-13" version="1">MPMLRGRPGHTYGRPEPLSSSSAPRDISEARFASAWQYRYSKIDETRSSKASGRVQKWASVTTEDTSNDDAALQICPSSKQDHADVRYAVVREVGRVLEWCRKIDHRSVRAAGSWLSSKRGPSVVQCFHQNDVRAHPEVLHPRFPAKQNFPPSIYGLVLVQSPLLKEVHCELVQSAHPHQKPEYHIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RC76</accession>
    <name>A0A5B0RC76_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_003828</name>
        <name evidence="6" type="ORF">PGTUg99_011101</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000209">
        <property type="protein sequence ID" value="KAA1123346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RC76"/>
    <dbReference type="OrthoDB" id="2507267at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="375"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="342"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1083681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1123346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="409" mass="44578" checksum="A02846EFA3BCC98B" modified="2019-11-13" version="1">MSNDKEEKKSLYPSLPPVGWTAGNPTGNPTPTGASATDPLAPPPPANGKKYTKEDIAGMTPEQRQWLHGHQDLIDWKTVDLIGDGDDSVGSSLLEAAGIVSDLTKSLHQMLIDPKDPKTGGTATGETAAGASSSGLTEEDFMSSYLRTDSVVLDLHKETEISKILKGWPEKDCPKFTGAVGEDANDWLVTMGVLLGDRQAHLALWHVAAGQHLSGKAFRDHKDAVLAGTRPTDWKSFRSWLVKLSPLGVTPQSIADELERLKQGPNETFQGFYEHFRDWQTKAKSINFGHDERTCFVKRLTPGLSNKVQGLMSQEQIRNTPMTMDQVLATAMQHDYLFRQSKAAASTSGGSGSGKRRSESSSGGTEKKKTGPCTCHNCKKEGHIALKCPEPKTDQQKAWEAKNAEKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJ80</accession>
    <name>A0A5B0RJ80_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011023</name>
        <name evidence="3" type="ORF">PGTUg99_050223</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJ80"/>
    <dbReference type="OrthoDB" id="2497033at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="368" mass="40118" checksum="5CEAC14C8249A23D" modified="2019-11-13" version="1">MIFTAKHQQLFTIKSVKTGRRVGERVLLDILIAHRSLVLLRRCSNCYACNRSVRESDGMRPRGNCKVGRTGWKEKEYKRLSIPSGLLPTNHISTCAENQKKKKKKTDTSKIIIMLTPSHHLALVMSLLTGAWLARTSFAALNCAGGEVVDWNECTRAIATIVYDKPKNTLDRVSSRFAKLSGNCTILVNNPDREVVTKQQIEAGFKSILDACKPNTGSISLSDSVYVESQNHQSLHDTDYLAPTTLTCGLNSNAPLTVDKDCQDAFDSISVDRWGRLLGDKGKPAPSVLKTLKTCTVLIYTTDASQLIAKKAEIGAVVSKTIKQCKGQSGVIGLTKGGSGNNGLTVVKVRSSKRCGSRVDSEGQYCAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S3D9</accession>
    <name>A0A5B0S3D9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019783</name>
        <name evidence="3" type="ORF">PGTUg99_032816</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S3D9"/>
    <dbReference type="OrthoDB" id="2506276at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="458" mass="50612" checksum="6880DFA15676A237" modified="2019-11-13" version="1">MNFSKTTSFSPLHIRTADTPPKVEKVITPGRELTREERKLARRRNGPTDVLPTYAGKRLTFTLPPATGPKFGQLSQPDVIPSNDNNPPQSSNSTIPNTPSTSAFQKPRKVLKSPLGREISVADCAKPHPEPPDPSSSKKANGKSSKGKENAQPTSQGHDNLPSSASIKATVKKPVPHVSETRPSKASAKSGKSSQVVQRKEERSELSCDSEATPTVLNTPRGSPGSSSDSSKMKTLACKIFTPGVPRKEPKNSQKLAETSRKTDINSLFERPTTTSEAPKAEKNLAQESQQDPAEEQIYIVADRLLRQDDPLEKSIEDGVELSLETQRLLLKIKSQLNPPDIISISIRKLIQDELEKVDQRLLLVSDRRDEGDSEDEDEGEDERIEAESERAALASLLENFKTEMLDYSDQLVRYGLLKVKLQQLQTLETDLQSQLQPSTSSQQPAKRKPPKKKHRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S456</accession>
    <name>A0A5B0S456_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine desulfurase</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NFS1_2</name>
        <name evidence="13" type="ORF">PGT21_028358</name>
        <name evidence="14" type="ORF">PGTUg99_016057</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. Plays a role in both tRNA-processing and mitochondrial metabolism. Involved in the 2-thio-modification of both 5-carboxymethylaminomethyl-2-thiouridine in mitochondrial tRNAs and 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) in cytoplasmic tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000079">
        <property type="protein sequence ID" value="KAA1132548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S456"/>
    <dbReference type="OMA" id="KGLYWAR"/>
    <dbReference type="OrthoDB" id="10250117at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990221">
        <property type="term" value="C:L-cysteine desulfurase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000003">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000002">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02006">
        <property type="entry name" value="IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002806">
        <property type="entry name" value="PRK02948.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010611">
        <property type="entry name" value="PRK14012.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="12">
        <location>
            <begin position="124"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1113335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1132548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="523" mass="57417" checksum="6BC15EF18F57B054" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SDC0</accession>
    <name>A0A5B0SDC0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006411</name>
        <name evidence="3" type="ORF">PGTUg99_016493</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SDC0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="97" mass="10901" checksum="0AC4BA392E2A2E98" modified="2019-11-13" version="1">MDSIKVSENTESQIKTHSSFINPTQNQQFVAHTVIQANGGCSAEDERRVESPTTMAKTSSANTDSQATAIQQIRPFPRRFFFPLAPLVDQYNFPIPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LI01</accession>
    <name>A0A5B0LI01_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000200">
        <property type="protein sequence ID" value="KAA1063989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LI01"/>
    <dbReference type="OrthoDB" id="2516992at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1063989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="221" mass="25539" checksum="676DD60D924E6BFD" modified="2019-11-13" version="1">MTIHRHQNWIKQLFNGHHATYQLDDSSRRRELMDIIVTVAREPNRGFEVIGVAYDASMFSIDQINFQLSSRDMAALVARFRNVLNLSHVHDLHQFACLELSDHLQDSSLPRESYLLHTYAKTHCPTRTQNFYPTRSSYLGYLTKRGRNYGVCQTRTDSTFWMVRISRTLIPEDLDHWGTDRPTMAMATRDQSRFIRTVVARIPPAPRSSLAESTPSQLAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJ14</accession>
    <name>A0A5B0LJ14_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_027107</name>
        <name evidence="9" type="ORF">PGTUg99_006819</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-histidine(out) + L-arginine(in) = L-histidine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71063"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the laat-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000519">
        <property type="protein sequence ID" value="KAA1063870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJ14"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000009">
        <property type="entry name" value="PQ loop repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000028">
        <property type="entry name" value="Vacuolar membrane protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF34">
        <property type="entry name" value="LYSOSOMAL AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="328"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1063870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1099974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="462" mass="51401" checksum="EE3E70E3ED28BF1E" modified="2019-11-13" version="1">MLVSDIFGNLSQLFWLFAQLPQIWTNYRNKSVEGLSLPFLLNWLSGDVTNLIGCLLTNQLVFQRNLAIYFNLIDLILIGQFALYSRTTTSTTSTTGATTSSNNQTNQHAYAPISQTHLERSHPNHPIDIERSTTNSKLGLDLNHNNRHSDQSTIKAACPQLLHPAPHPSFQSNQLLNWNENSINNPSNTSYYTPSRFTRIIRCCHLKPLLFLAIVAFIGFQITTHHSSFSLGDHKSPDSFAQISRSTFGANNPTQNLSPTSEGVIEKTEREAQDTLEVIQETNKGEKPDSLKQKIGRLSAWLCAFLYLTSRIPQIMKNHSRKSVEGLSILLFVLAFLGNLTYVLSILSSPQILLNNPDDANRDHRKLDFLNEAIPYLIGSAGTLCFDLAIFIQSRLYRSHPTQPSPSSTLPDSPSITCSNLVNPSPPLLSPSTHPNQARLNPDPEIYAALPLLDSRSSTSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJZ5</accession>
    <name>A0A5B0LJZ5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJZ5"/>
    <dbReference type="OMA" id="MNIPTNE"/>
    <dbReference type="OrthoDB" id="2506636at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022791819" description="C2 domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="16463" checksum="B0220B70136B544A" modified="2019-11-13" version="1">MNPFNPKITLLVITTLLLSLCTSAKKRKCRATLNSDAYGNSWEKANVKWKSPKHQKEGVVEATIHGRMDIPTNEGSTIKSHVCYATLKVNLTDCSIVLGKNDTADQGWDPHHSPIYRNQDTWNWNETLTFRPDCPENIHVEVFEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKM7</accession>
    <name>A0A5B0LKM7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015337</name>
        <name evidence="1" type="ORF">PGTUg99_019736</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKM7"/>
    <dbReference type="OrthoDB" id="2508746at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="92" mass="10245" checksum="466C6BBF42C92E22" modified="2019-11-13" version="1">MDDPSTYRGLVFPCATGESGHCGLEIKQQAIFYMLYARKSTKPPITFDCNEQSGTTPYCCNDKKFSYTDPKKNITSTTIVKNTCSIGKKSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LL18</accession>
    <name>A0A5B0LL18_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_020335</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LL18"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
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    </evidence>
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    </evidence>
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    <sequence length="774" mass="86014" checksum="36B2E8EB57119A75" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
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    </evidence>
    <sequence length="116" mass="12488" checksum="8D35DAFFE198A428" modified="2019-11-13" version="1">MLIAAAFGGAVPVDHKGARYIVSDLNFPSGYGAPSISGQMQHGQRILIPYVNHGTVDLRVWVAGHQVQTIKPNHSIQYTLNPGNSLAVESSTPGAAFDQQTIQYVIQRAGFTRSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
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                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="251" mass="27574" checksum="19A21B3D2BDAA314" modified="2019-11-13" version="1">MPTRLISAGGFVLAALAGLAGARVDLGGGHMGLAMARTSCSGAHRVACTELDDDWPDMGLACPLAHHDIPRHTLAMMSATIIQLGHYKPVPVDQFPSHQCYTTPPNATVSSYGFISQGSFDMWFFAHDKCDCEEEQMPKCGISATEKEKCNLRTFTLCKVTKKTEICKPKHHPGWKPISDSSGVRKATDQERAKEEKAEEGSSSSPNGTVAEAEKTIRFGPAHRQQPVLMFADLSSFWWRIMELLGFWGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0LPF4_PUCGR</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
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    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1128410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="123" mass="13321" checksum="EDE78BCF791AA418" modified="2019-11-13" version="1">MHVLFAGGSATHKVTTSKITSTASPPAKITQKRIYKEASPSPIPPIITQPVHTQLAQWSSPSSLSSPAYAASRHPRVSAIYSNSIILILPQATFDLPIILLPFLVAHHHSLITHQSIKNHQGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPN7</accession>
    <name>A0A5B0LPN7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPN7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="266" mass="29256" checksum="425723C5B8B09AD9" modified="2019-11-13" version="1">MDHKAIMEMFDIYMGARRSESQQQPARPSRSERHRARREAHARNPPALSFLEAWNAPLVQDATAPIPQAAPAPTPQAAPAPTPQAAPAPTPQAAPAPTPQAALAQTPQTAPDQTPQAAPVQTPAATSSPDESVATLRAKHRPKGERLPFVEPQAKMSTLHDLRASWFSCPTAHPGGIRHPRVFTLIGRDDRVPAQQRGTLPRPTAASQVVLPRRARRHTPRRMPRPPQAQPRQAAQAQQARRSRRTQPYANSRRLSLFGLRMQRPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LS54</accession>
    <name>A0A5B0LS54_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LS54"/>
    <dbReference type="OrthoDB" id="2499658at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="49310" checksum="444DDA3F9FB74CC0" modified="2019-11-13" version="1">MSFPLNISTAQPNRFVEAPATGVKQNALASCIDGSDGYPTLQSFTSHMETYLSGKKRADRTVITSADLHQIHRLLLDPAALRADPNPSNRAWVKKTFFLEPTPSGTIVCHREKGQHIGRPVAVKELMYFILKEAHASEGHGGRDKTAKAVKNTHSYIRKGLICIFLETCPTCQTRIDAVKKEKNAALAKSLNMSASRDSIVSFGTGFLTPATSPTNFGNKRRGSMFQSVPQSVDSTESPYFAINKPTIPPELALVPAAPKPYSRSRNASYPSESYSNLRKGQRMLSSGSNLGGYLPIPTRPLHSPSVIMPNTAIASDFSSQDPSDQRKQRASSNCNLPTSITQGLAPNSQNPESFLGEPSAWSYQPQTPIFSLGNHSDNSSWEIEFANIWSGNEPNFYNPVPAPLLAPQPQFSASERLKIPFTDQYVSSDNNNLLFNSWSSVSPMTNVFPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSF4</accession>
    <name>A0A5B0LSF4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Charged multivesicular body protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_008159</name>
        <name evidence="3" type="ORF">PGTUg99_032675</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSF4"/>
    <dbReference type="OrthoDB" id="10250120at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF96">
        <property type="entry name" value="BCDNA.GH08385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="465"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="512"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1071460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="59149" checksum="7EF6E88BD32D152F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LTU9</accession>
    <name>A0A5B0LTU9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LTU9"/>
    <dbReference type="OrthoDB" id="10441619at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="140" mass="15988" checksum="4C471A2D264A8654" modified="2019-11-13" version="1">MGKERKLGELERQQEASRAGRRRQERSKDADSASLKALTTDPTALSTSTSETTNPTCQPTTSKKRKQKSKTSAGIEEKFLRGERSDSEDPETLEQPRKSEGLNEIMKNVKQRASQGRNTSRELEWEWKPNMGVSRRKFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWN1</accession>
    <name>A0A5B0LWN1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein cwh43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_024640</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1068004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWN1"/>
    <dbReference type="OrthoDB" id="68581at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000031">
        <property type="entry name" value="Calcofluor white hypersensitive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057315">
        <property type="entry name" value="Exo_endo_phos_PGAP2IP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051916">
        <property type="entry name" value="GPI-anchor_lipid_remodeler"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053912">
        <property type="entry name" value="PGAP2IP_TM_1nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053911">
        <property type="entry name" value="PGAP2IP_TM_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859">
        <property type="entry name" value="CALCOFLUOR WHITE HYPERSENSITIVE PROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859:SF1">
        <property type="entry name" value="PGAP2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23022">
        <property type="entry name" value="6TM_1st_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23021">
        <property type="entry name" value="6TM_2nd_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23226">
        <property type="entry name" value="Exo_endo_phos_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="448"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="501"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="539"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="575"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP first transmembrane" evidence="5">
        <location>
            <begin position="160"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP second transmembrane" evidence="4">
        <location>
            <begin position="376"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP C-terminal nuclease-like" evidence="6">
        <location>
            <begin position="610"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1068004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="897" mass="100735" checksum="4DDFD26F1738657F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M033</accession>
    <name>A0A5B0M033_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_019777</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M033"/>
    <dbReference type="OMA" id="IGTGTRW"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018455">
        <property type="term" value="F:alcohol dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016652">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19120">
        <property type="entry name" value="AKR_AKR3C2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000044">
        <property type="entry name" value="Aldose reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044494">
        <property type="entry name" value="AKR3C2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="12">
        <location>
            <begin position="36"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="11">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1069288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="295" mass="32339" checksum="DFB1DA70A295CD86" modified="2019-11-13" version="1">MAASHYITLNDGQQIPRIAYGSGTAWFQATGPDGVSSELVEATSAAISIGFNHLDAAKVYGNERSTGVAISKCGLPREKLFITTKIISGLSSVKETLKEQLRDLQVEYVDLYLIHSPMFTKEAGEPTLAEAWKKMEEVHAEGLAKSIGVSNYRISDLQEILKSAKVKPTVNQIEFHPYVWKEAEPLLKFMKEEAIVLAAYGPQVPVVKLPGGKVDPIIERISSERKISTGEVLLKWAKAHGAIVVTTSSKAARMKSMLETVLDDKSDLSPAEVQELNEAGIENGVQRIFMKHMSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3F5</accession>
    <name>A0A5B0M3F5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3F5"/>
    <dbReference type="OrthoDB" id="2506420at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="302" mass="34390" checksum="A9538CA0DA2C3086" modified="2019-11-13" version="1">MKQSGMIQPSPDSRQSITQPLLSERPDINPRSQSKKRTEAPTNSDSTPQIPPDSNEENAKAKFKRQRKNPEEDDIKVFFSEPFRRKDDPADKPPSTYSCLWCKKEVRVSASSLSNLCVHRDGSRQTGRVSNGCPNRAKAIAAGAKLPQMALDEEKKKKGTQEITSHFARVEKFDNIILNRIVALWLLRQAIPWNQVEDPYLQAAFNYCEPAANFFKRKWAATASRTAYLELQEEVVRQIKATNSKFNLIHKVRTTKGNRYGFIGVAASFIDNNWNYFLLHLGLKLVAWFHQRDLLAALLSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M623</accession>
    <name>A0A5B0M623_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013221</name>
        <name evidence="2" type="ORF">PGTUg99_021422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M623"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="59815" checksum="557FE1B83AE4F885" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8T6</accession>
    <name>A0A5B0M8T6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8T6"/>
    <dbReference type="OrthoDB" id="10357625at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023071242" evidence="2">
        <location>
            <begin position="23"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="167" mass="16503" checksum="47FF26F01698FA72" modified="2019-11-13" version="1">MTNNALISVAIILCALLQGISTRPAPAEQQPKGANPTAKMFGSTVPSDGPPASGNAGQIIVGPFYQALDLNSQPIILQAVLGADGQPAIALSSSSGYNIGASALATGPAGPPGGAAPGTLSQKSVGHTPSDEESTPKTDATKLESLSLSSSLSHKGRSKAMAQSSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9F8</accession>
    <name>A0A5B0M9F8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005585</name>
        <name evidence="2" type="ORF">PGT21_011343</name>
        <name evidence="4" type="ORF">PGT21_034036</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1073415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9F8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1110902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="305" mass="33947" checksum="3C57FAF363D7F11F" modified="2019-11-13" version="1">MRPSPPSTDPSPGTYHSYPMSTPPTTNTGVGMNVPEPRAPHFQPPPYRLNPPRFPHPIFFPHPPMFHPPGQAFPPFGQGGGPRGRGFVGGRGGGRGVGRGRGWLPPAQLRIRGQANRFYYPNGPAAGVLFGHGHAPVDMDNFLHQHPIPPAVQRRPTPIPPVVQRQPTPISVPSSPSVPAARAARAPSNLSSIQSGDSFHTAEDFLAPQVRVESPTPAGMYDPDDYHLLARHQIESLLDRRAAPERFDRYEFTPQHFDNFYQSATSALVHHWTRHPAANANERQDRVRLFQFITRRYRTQTRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9Z3</accession>
    <name>A0A5B0M9Z3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9Z3"/>
    <dbReference type="OrthoDB" id="2507116at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022927253" evidence="1">
        <location>
            <begin position="20"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="159" mass="17508" checksum="52DD59E00C2E3421" modified="2019-11-13" version="1">MNCLLYAACFLAVLQSALCTMPTLAPRADQVAAAHKGQSDEKCYLGCLGWFGNTFNPWIFGNSYLTYPYYDYSFGLNNGYNNLAFAGGLGGGYGMFKKDASEPNKHQETSVCLASFFVFRIVQIIATDSLQIYSGLGSSCSRLEDFLITNYVSQQQLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDQ5</accession>
    <name>A0A5B0MDQ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011059</name>
        <name evidence="2" type="ORF">PGTUg99_014969</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDQ5"/>
    <dbReference type="OrthoDB" id="2498190at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="573" mass="60945" checksum="E0C05ED46200B5C8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDY4</accession>
    <name>A0A5B0MDY4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDY4"/>
    <dbReference type="OrthoDB" id="3066634at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="140" mass="15280" checksum="FC41E821731F0E4A" modified="2019-11-13" version="1">MKAWPTGTLCQNSGFNYLPDPFSQICLQEAFWESKHDPNTPIALWIGHVRVATDDLLTIKKLPTDQQIADQIVAGLDSSWAAVKDSIIYTAQEMSLNDTIGVLEAHEVSLHGRPSSNFASASYASNQCTGCSNCGKRGHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MET2</accession>
    <name>A0A5B0MET2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MET2"/>
    <dbReference type="OrthoDB" id="3344688at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="13182" checksum="BFECA0B1E3C24119" modified="2019-11-13" version="1">MSSTESEMNALSDGEQESQWLEFLVEELWRKTLAPTLFSIDNKDPSDETNSHSPWGRSHSKPATKDKTKNDGDEKRRTATPSIAHRISQSTGPELLARINQEHHEPQSEQEANASQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MG44</accession>
    <name>A0A5B0MG44_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DUF125-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_028824</name>
        <name evidence="9" type="ORF">PGTUg99_030558</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000171">
        <property type="protein sequence ID" value="KAA1126619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MG44"/>
    <dbReference type="OrthoDB" id="73465at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02435">
        <property type="entry name" value="CCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1075103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1126619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="43225" checksum="D0647FC167A1B306" modified="2019-11-13" version="1">MVETGKPVIQESSSTTSAPSTSVPLPKNSSLRPSASSSDNTENPNQKQKKVWDYYSDEAPSRPSEQSEADNALKPSSSIGSLSSSSNTHTKNNITPHLNKCDKKSRPEDIQVCCRELRGDDERHLINPDIVRDVIIGLSDGLTVPFGLTAGLSSLGSSRLVVVGGIAELISGAISMGVGGYLASEAERDHFKYLQKTTKERVARSCSGEMEREVHEVLGPMGLDESISRTVAAALLRAESEADECIDRTAEPAGESTARWLLRCLGRQPKFFKQIDGGNHQLRWAKDIGITPFLLKFGEGLEEVPESRLYISALTIGLSYFIGGLVPMGPYFFIESAQIALYWSIAIMFITLLIFGVFKAYFTGAKIGFVGYLKASSATIVVGGAAAAASWLIVKVLEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHK6</accession>
    <name>A0A5B0MHK6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COX assembly mitochondrial protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_035132</name>
        <name evidence="4" type="ORF">PGTUg99_036461</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for mitochondrial cytochrome c oxidase (COX) assembly and respiration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CMC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHK6"/>
    <dbReference type="OrthoDB" id="6224010at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013892">
        <property type="entry name" value="Cyt_c_biogenesis_Cmc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22977">
        <property type="entry name" value="COX ASSEMBLY MITOCHONDRIAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22977:SF5">
        <property type="entry name" value="COX ASSEMBLY MITOCHONDRIAL PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08583">
        <property type="entry name" value="Cmc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1076152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1091521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="97" mass="11366" checksum="7E11B8900CAC7658" modified="2019-11-13" version="1">MEPAPQSTTFQPLSRREEDEFQKRIKLAALKQCDPVVAAFASCSSTRTISVAWACRKEWKAVQECMQIHMTEEKLDLARREYMTKREELRVPYKKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJ42</accession>
    <name>A0A5B0MJ42_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_036461</name>
        <name evidence="9" type="ORF">PGTUg99_037599</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJ42"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="49"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="295"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="720"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="774"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="813"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="516"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="674"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="740"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="774"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="827"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="883"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="914"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="973"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1000"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="149"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1076268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1091641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1021" mass="113271" checksum="7434EB165AC173EB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJY8</accession>
    <name>A0A5B0MJY8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025160</name>
        <name evidence="2" type="ORF">PGTUg99_004206</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000247">
        <property type="protein sequence ID" value="KAA1118542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJY8"/>
    <dbReference type="OrthoDB" id="2508972at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="205" mass="23814" checksum="6DFDBC1FB5769928" modified="2019-11-13" version="1">MPLSALPLQEILRHKPTPLDLNRLASTGSEHISLSEVLLFSNLPTKKKQKVERMIMIKHYKSMITLWMTSLIANRLGFLPEDSVEVRHWCSPRELLQPRYNPVRSLITDTVGMQFTDRSGPEWAFFHETEDHAARAQFQWCDSEQAGLIHNVGKTEILYGLRHERTSYWVIGSPLLPGQTQVVHFPDRCFKGKNWLYIRTAVYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML03</accession>
    <name>A0A5B0ML03_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006751</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML03"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022995104" description="CCHC-type domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8385" checksum="AA201F6763042EED" modified="2019-11-13" version="1">MRLNIPIFLLAATLFSTSLAMHPLAEHEVVHTEFHPSPHQGHHEGGSGQQLDEYQACSHCGSERHDSDECPEAPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMZ1</accession>
    <name>A0A5B0MMZ1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)</fullName>
            <ecNumber evidence="2">1.2.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_005437</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:13458"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMZ1"/>
    <dbReference type="OMA" id="SEAYYMA"/>
    <dbReference type="OrthoDB" id="878at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007584">
        <property type="term" value="P:response to nutrient"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42980:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42980">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="6">
        <location>
            <begin position="99"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00051764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1077384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="432" mass="47687" checksum="A18AA5EDFC12BA8A" modified="2019-11-13" version="1">MVNRGTHQARHWLNSTTRRTTTTINPQSHHPRRPFTHRPPSQDKQQSSEPLSPAYPTLSTSAFLCHQPEDVERIRGLTRSHAGQHPSANQPPPPPPVKLNMFQAIRDALAITLQKDDSAVLFGEDVAFGGVFRCSLGLSEEYGPDRVFNTPLTEQGIAGFGIGMATMGHTAIAEIQFGDYIFPAFDQLVNEAAKLRYRSGGKYNCGKLTVRTPVMAVGHGGLYHSQSPEGYFQQASGLKVVIPRSPSQAKGLLLASIREPNPVIFMEPKVLYRSSVEWVPGGEYELALDRAEVVSAGQDLTVVSYGTAFYVCELALAMLRNPPPEIAHLVPQSLRNLSVELIDLRTVVPFDYPTVVQSVRKTGRAVVVHEAPLNGGVGAELAARIQEHCFTRLEAPVKRVCGWDTPFPLVFEKFYLPDQIRILDAIVETMKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPH3</accession>
    <name>A0A5B0MPH3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022524</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPH3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="211" mass="23791" checksum="742F77D0C862C1DE" modified="2019-11-13" version="1">MEDREERDEAREQLSQAQDGPFNPHVPLPGRHTPLSSANSSPHPANSPCTTMGPGCGEDVSGSPPPVVEPEELSGLSRHVVCKLFLMMRGLVSRAEGQLACFENKLKNDESNFRDNISNEFDRHKMEMRNLLADHQLMAVRDHLLKEIERHKNRMSCVISGRNIELLVHSDRRRQLRQTDDRLSTQMLEMLEASATESKGATSSDTKIDQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPR1</accession>
    <name>A0A5B0MPR1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_026265</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPR1"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000026">
        <property type="entry name" value="GTPase activating protein for Arf, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF1">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="3">
        <location>
            <begin position="13"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="419"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="461"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="483"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1077994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="504" mass="54808" checksum="0B2F7D2E963DFE79" modified="2019-11-13" version="1">MSAPTKATAERHQRILLELLKQPGNEVCADCQTRNPRWASWNLGIFICVKCAGIHRKLGTHITKVKSLTLDSWTKEQVLSMKQLGNLKSNQIHNPNGSRNPPPTDLEESERDSQLEKYIRTKYESKRFMDSHRKPGSISSPRTSDSSAVSFTMSTTSPAPSPASSVFKQSPRSQLSIPARSSSVAGARAIDLSTRPPQRSPTTANAQRPIAPRTMTSALSHPSTQIQPVSQDRSSVSHNPQVPHPPPRPSTAPIPSDGYHSSQYAPPVPTVPSLYTNNPGYQALQSSMAKNHNATLFNQPALHHQQSLNIQLQGNQPVHQVHPSPAPPPRTDGVWGDLMQLTQPHLNHPSQPSASFLDSSSLNLSPNMANPNSYQPLHSTTPANNNMNGLEVGMGRLMYDTPSTNPFSSQNYASNNRTGAMAAQSQLHPQASYQAPSPFTVVHPPQHSGNPFFAHQTTLHSAPSSSSSSSAPFANGLAPNRNPFLSTNNNGQPFLPHSTDFSHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MS18</accession>
    <name>A0A5B0MS18_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MS18"/>
    <dbReference type="OrthoDB" id="2498944at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="553" mass="62771" checksum="BA390073FC900B08" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTF6</accession>
    <name>A0A5B0MTF6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Proline dehydrogenase</fullName>
            <ecNumber evidence="2 5">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_020810</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the proline oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2 5"/>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTF6"/>
    <dbReference type="OrthoDB" id="5464at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="6">
        <location>
            <begin position="191"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1079683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="399" mass="42822" checksum="3857D077E71D39EB" modified="2019-11-13" version="1">MKALYEGEVGSLLNYSVEALEGAGKGSSESGLSRESVAAITRTVESLAGYESGAKGYAASSARMKPSAVAIKVSGLVDDPYLFKRASENLLANGLSPFRSQRRLSEGQSGRHSCVGQNSYLPLGRIQQIEIRGTWVGELLTDGLQHFPGAPTVHAGATSRICRRGAQAGLFGGCEACPRAYLVSETARWREARAAGRPGVPDHPPVWASKAETDGCFDNLASSLVQQLASNARRAQGLTSHPRQSRSSNSIEVAMMIAGHNPISAAKVLKQLRDEEGLAKNVDAQSIRLSDSLRGRLMFAQLYGMADNLTSTLTQILARVTPIIKNTHSHSCSSIYHSAQWTRSYPISLVGQKRTHLSSKSKTANPSSVSNVNSLARRSVNESDLCFSSLKDSPNHFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTH4</accession>
    <name>A0A5B0MTH4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTH4"/>
    <dbReference type="OrthoDB" id="2509372at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="182" mass="20791" checksum="B45E95669DAF75D5" modified="2019-11-13" version="1">MTTLTRKEPAAPTQGKSTKTKDDPPPNTEKDTPMDVDEIESECSEATPKRPSTPEIRILTKEEQIRLRVREHVSLWKQCQLATREGPTAKLRALLTTAQESQKTLQKLIDNDEIESYVKGWNPWDEKKINFPSPPKKNLMKFKKNEPGKRQSSSSMNFSDPVKWKKATDLLQIAAGLYQNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUB9</accession>
    <name>A0A5B0MUB9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUB9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="136" mass="14739" checksum="9B3E7AFF8B075136" modified="2019-11-13" version="1">MSSQPTQSSMSNQSGNVSNATEVQTRRSSRVTTPMKRSGMIQPSPDSRRTITQPLLSERTSIGTSSQPKKRTNAPSESDSTPHATPVQSQQTRKKVPKKPKKTGPAAATSESNTTAKDVDLAQDSDEENSKVKKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVA0</accession>
    <name>A0A5B0MVA0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002208</name>
        <name evidence="1" type="ORF">PGTUg99_011572</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVA0"/>
    <dbReference type="OrthoDB" id="2495683at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="201" mass="22994" checksum="2815D21A87511137" modified="2019-11-13" version="1">MKVQNSEGIRAYFPIHGIHALCSRIEYPPIVQEGDCEKLAYGAKHPGVILSRFFARSQWKLTLAKLQRNPNDSGIRARLTKLRERVSKLSNFQNHVKDRNLDANYLSVSISCIDTCHTTFALYSRPTIITVGIKQLRRANGYHSQGIYLSEDCLWSVTEEFVFRENNRVWCCNTVPPLSALLEIPRIAIHDSFFLAVQIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYN0</accession>
    <name>A0A5B0MYN0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable 26S proteasome regulatory subunit p27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NAS2_2</name>
        <name evidence="5" type="ORF">PGT21_029117</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYN0"/>
    <dbReference type="OrthoDB" id="72325at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000107">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040815">
        <property type="entry name" value="Nas2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035269">
        <property type="entry name" value="PSMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18265">
        <property type="entry name" value="Nas2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="197"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00068021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1081088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="317" mass="34582" checksum="0DC17705A7DF00AF" modified="2019-11-13" version="1">MPTLEGLGRSPKFSGRLQCTQSRPHRPVRLGGFDGPGQLVGAPGPIMGFDVKFQLKTTPQPDTTTHSAELFIKTFSVKPMMGRSIHETTRRSPHDISTVSDESTPAGRVAALSEQKRQIETELHQQYDVLSVNSIDLTSPLTDVNGFPRDDVPDLASVRVARARVCELKNDHRAIVDQLAQTLPLLLPKEDHSSATPIKGASTNGSSSSSMVPFAQIDVVAPDSPAEQAGLKLHDQIVRFGHLNAQNHDRLQALAKLVAESEDQNITVVCFRFEEGNKTMISKELKPHSGWGGRGLLGLSGFLLFPVMFTPTVMRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZC9</accession>
    <name>A0A5B0MZC9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004499</name>
        <name evidence="3" type="ORF">PGTUg99_032362</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000439">
        <property type="protein sequence ID" value="KAA1082305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZC9"/>
    <dbReference type="OrthoDB" id="10384433at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1082305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="383" mass="43482" checksum="0D2AEF4E6B3D0E8F" modified="2019-11-13" version="1">MIISLEHSFLCRIPIPQDLRYPNRLPENRTYLRVGKMNRTTPKANNNHVPVHHPRTNSPYPLSPKTISLSCLALFMLEVALVEAMEFSATAGRSAKFANHTPKAGQESSATFLESGTASWSKKPRVENEVRLARGGPSPLAEEQLKNSGDPSKLENLKKLSGIQRFWKKLMRILMAFFKPTKPVKEGQFPKSKIPMKTVPIPGNQKIQLVTIYKGPIFQTQDFIKKDEFNVYRALAHLQYQDQEKHGIVANQIKTYIKENPVQFKKYEILTEDSTRTKKVANQVIDIGGHVYANLKAQENRNVIFSAFSKSQSERLGFSVFTKIPEPHDPNVVKLSGYHYTSIKNERENPRQGLIFENGHYEVFGHSKSAVFVNKVSAEVNHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZP1</accession>
    <name>A0A5B0MZP1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZP1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="91" mass="9628" checksum="BE4EA777143B4933" modified="2019-11-13" version="1">MHSYSAKAVNPAPNPDPAIPQSSRTPSSQPDQSLKVSLNRPHNGGAFDCPLSRAASVSLILNDSILDPSCGLTSSPEDLLSISLDHYRSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3W8</accession>
    <name>A0A5B0N3W8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C-8 sterol isomerase</fullName>
            <ecNumber evidence="8">5.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Delta-8--delta-7 sterol isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ERG2_2</name>
        <name evidence="10" type="synonym">ERG2_1</name>
        <name evidence="10" type="ORF">PGT21_005013</name>
        <name evidence="9" type="ORF">PGTUg99_035862</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Steroid metabolism; ergosterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ERG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3W8"/>
    <dbReference type="OrthoDB" id="347124at2759"/>
    <dbReference type="UniPathway" id="UPA00768"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006716">
        <property type="entry name" value="ERG2_sigma1_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868">
        <property type="entry name" value="SIGMA 1-TYPE OPIOID RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868:SF1">
        <property type="entry name" value="SIGMA NON-OPIOID INTRACELLULAR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04622">
        <property type="entry name" value="ERG2_Sigma1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1083542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1093972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="265" mass="29397" checksum="B4D993C765CCA517" modified="2019-11-13" version="1">MNNRSVSGPRLPPGSRPKAIHIQKQIPSSSLAAITRRTLLLTIFSALFIGVYRWADAIKSRWYIFDPSELHKITLEAIERYPNSTSAVIHHIVQSFESQPKLKPYITANPFPDPSPQAVSPYPSEWVFNNAGGAMGAMYIIHASITEYLIVFGTPIGTEGHTGRHTADDYFNILAGQQTAFAAGSLEAEVYRKGSVHHLRRGQVKQYKMPDGGCWALELAQGWIPPMLPFGLADSVFSTVDLITIYHTIRITAREIIRNLVAGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5Q1</accession>
    <name>A0A5B0N5Q1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_007547</name>
        <name evidence="5" type="ORF">PGT21_018292</name>
        <name evidence="2" type="ORF">PGTUg99_002377</name>
        <name evidence="3" type="ORF">PGTUg99_030212</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="2 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000512">
        <property type="protein sequence ID" value="KAA1065128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1067070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5Q1"/>
    <dbReference type="OrthoDB" id="2509876at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137380" evidence="1">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1083803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1119218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="107" mass="11968" checksum="C749153EF16D5D73" modified="2019-11-13" version="1">MLLKWHSIILSLYCFQLACVTAFIPPQLPGKCPTCKSDYVSMQIKDIGTCNAIFTCTHARQVGTDNCGIMGRLVHRHCSASDDCWDSKKTYKCNSLHLRPCPPSCDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDS3</accession>
    <name>A0A5B0NDS3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_032005</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the asparaginase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDS3"/>
    <dbReference type="OrthoDB" id="542841at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006528">
        <property type="term" value="P:asparagine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08963">
        <property type="entry name" value="L-asparaginase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000003">
        <property type="entry name" value="60 kDa lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:001156">
        <property type="entry name" value="L-asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.40:FF:000001">
        <property type="entry name" value="L-asparaginase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041725">
        <property type="entry name" value="L-asparaginase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00057">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="9">
        <location>
            <begin position="15"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="10">
        <location>
            <begin position="309"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="580"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="613"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="81"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1087461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="652" mass="71486" checksum="3D516C11611FB825" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEQ0</accession>
    <name>A0A5B0NEQ0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001807</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEQ0"/>
    <dbReference type="OrthoDB" id="2495662at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022698">
        <property type="entry name" value="OrsD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12013">
        <property type="entry name" value="OrsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="607" mass="69057" checksum="E2C76D842A8904D4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NF98</accession>
    <name>A0A5B0NF98_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA polymerase II mediator complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SRB8_3</name>
        <name evidence="2" type="ORF">PGT21_025410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NF98"/>
    <dbReference type="OrthoDB" id="20828at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="752"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="687"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="752"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="839" mass="93638" checksum="E5F9C343676B43E2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHA0</accession>
    <name>A0A5B0NHA0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Plasma membrane ATPase</fullName>
            <ecNumber evidence="10">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PMA1_6</name>
        <name evidence="12" type="ORF">PGT21_025650</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="10"/>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHA0"/>
    <dbReference type="OrthoDB" id="2501025at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000008">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="681"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="749"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="792"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="821"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="854"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="11">
        <location>
            <begin position="48"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1087209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="959" mass="104453" checksum="16EBE4EBD73EC0B3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NM51</accession>
    <name>A0A5B0NM51_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025662</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NM51"/>
    <dbReference type="OrthoDB" id="10591693at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="39382" checksum="4BA37F7A67BC70A4" modified="2019-11-13" version="1">MILNKLNQLMTPYYDAKASTTNDSDTNTKPKGRPIFILYNVLARICREGKKESQCQKLKADHFKPIMMNIHDLNYTRLKRLVIEHIREQSQQQSPPYPIDVIAVYAEYESALQWYYAAVPKSSLVPVDFVHTALTESSFDAFVKEVENAPDDYRLWIEICMCSLNKIEKPEAIISIMVSRPIVKAPHGRRSLPITFQAPDQESQPYAATNRQLSAGVAILDLSNCLIDDVSYVPIRRDSESSNQLPAKKRIRFNESTSNMSLDDFLGLCSIPIDNKHVREMIETHEILDWQVFIGTSLTELTQLGFKWGPARSILAGVAKATESPAPKPPSPYVSDIVSKQNLKSAPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMA8</accession>
    <name>A0A5B0NMA8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TspO/MBR-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_017165</name>
        <name evidence="7" type="ORF">PGTUg99_008346</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TspO/BZRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000385">
        <property type="protein sequence ID" value="KAA1090421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMA8"/>
    <dbReference type="OrthoDB" id="8841220at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033013">
        <property type="term" value="P:tetrapyrrole metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15904">
        <property type="entry name" value="TSPO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.100:FF:000001">
        <property type="entry name" value="translocator protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="entry name" value="TspO/MBR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004307">
        <property type="entry name" value="TspO_MBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057">
        <property type="entry name" value="PERIPHERAL-TYPE BENZODIAZEPINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057:SF0">
        <property type="entry name" value="TRANSLOCATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03073">
        <property type="entry name" value="TspO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1090421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1105734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22593" checksum="28505954985B9985" modified="2019-11-13" version="1">MSLRCPIPATVLGLTSNPVIAVLTPVTIGSLIGLVTKSSIKTWYTTLKKPKGQPPRWAFPVAWTYLYGTMGYASHLMAESLRTTRFIETADLANSALTLYFGQLALNFIWTPIFFGLKKPAPALVDILTLTGTVFYMTNLTGKFNSKAAYLFLPYCAWLSFATYLNGTIWYLNPGDGPPSTIRSAIDYLVSKFNRSSLPKQKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NN78</accession>
    <name>A0A5B0NN78_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NN78"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1090725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="114" mass="12859" checksum="5243898219E64DBB" modified="2019-11-13" version="1">MCRVLRIGNPYLFARRSSIGQGHDEPKALWASKKSNIIPSCVPAHSLKKGKISRLALGPHRLMSRFKLLALHPPPKNMIDLLASHSFLAFFLIAKNLPQIHLDRPTTSSRHFNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NV51</accession>
    <name>A0A5B0NV51_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="14">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MNS1B_1</name>
        <name evidence="17" type="synonym">MNS1B_2</name>
        <name evidence="16" type="ORF">PGT21_005722</name>
        <name evidence="17" type="ORF">PGTUg99_009348</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="17 19">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="14"/>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NV51"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000047">
        <property type="entry name" value="Mannosyl-oligosaccharide alpha-1,2-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF101">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033473938" description="alpha-1,2-Mannosidase" evidence="15">
        <location>
            <begin position="25"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="326"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1092516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1093619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="556" mass="62256" checksum="088D37E398A9CC05" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYV1</accession>
    <name>A0A5B0NYV1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NYV1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="222" mass="25040" checksum="EC650FBE1A09A7B9" modified="2019-11-13" version="1">MLSGNINLRSVGHYPSFEAPLDQSDLNAESNLFLSGDNVIQAQDFPTHQDYTQSQYNSKDKSHCESLESGDGDITWKKVVDQSHWKSDYGLYLPGEDSIQIQNCPTHSDSPQCQTHNKNKSNCEALGLGDCYLAWEHQLDQPKWKADLDFSFCGEDLTGLQDHQTNQDYSQVADYSKHKIPEALMEGNTSGVPGISLTHSFNKCDKDSLEEIKDEKRCQKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZP2</accession>
    <name>A0A5B0NZP2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014688</name>
        <name evidence="3" type="ORF">PGTUg99_004131</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZP2"/>
    <dbReference type="OrthoDB" id="10413439at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="216" mass="25022" checksum="0D34B0714547B210" modified="2019-11-13" version="1">MPRLARSNRAAPSHTRATQDTNPIRFEGRVTRQVGAWAQVSDLAGFQARCQAVVHQVDDLLQYVVERPLTKDADLGFRPFQNLIPVIKLSRLFLNKLSRVSSDKPHPFSEVSPDELTTLIKAIVRLPDKINTFINRMEDHFSRIGVVYSEKVFDILDHLQRPIKIVKHLLDHPESPQGLHLQYRRWIDVWNSHLCRAGCRASRPHVTWKNDIHRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0G4</accession>
    <name>A0A5B0P0G4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MoaB/Mog domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013853</name>
        <name evidence="2" type="ORF">PGTUg99_024926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0G4"/>
    <dbReference type="OrthoDB" id="448496at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047884">
        <property type="term" value="F:FAD diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042726">
        <property type="term" value="P:flavin-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47675">
        <property type="entry name" value="MOLYBDOPTERIN BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47675:SF1">
        <property type="entry name" value="MOLYBDOPTERIN BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="100"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="373" mass="41957" checksum="5580B8928FC72EF4" modified="2019-11-13" version="1">MNHFPRTGTINHLVKRTKQAYRTGGRLIDHHRPFHSILASGSSTPSREVVPIRIRAGIINLASSVTKRKMTGGADEKEQLSFPVTPYPKSNPEKIVRTAACVIIGDEILSGKTLDTNSNQFASLAFRLGINLKRIEVVPDEEEVIISTIRRLCEPENEVDMIITSGGIGPTHDDITYQSLAKVYDPQGELEYDQETIRRMDEYNARRPDTKTKNEEQRMARHRMALFPKKHAKVLFVEAHLWVPVVCLRDKIFILPGIPSLFQQLLQALMCNYISLPPETDKPHRVIIQTKLPESIIAPTLTRLSKQLKDEKQDIKLGSYPNLITGIVSISLIGIDLEKLNSFAQLIQLEFDTLSSSSDSSKSNLSNNSENKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P420</accession>
    <name>A0A5B0P420_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyltransferase family 31 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P420"/>
    <dbReference type="OrthoDB" id="2187549at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000063">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006740">
        <property type="entry name" value="DUF604"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10811">
        <property type="entry name" value="FRINGE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04646">
        <property type="entry name" value="DUF604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="67508" checksum="8BFC89AB15A45C1A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P613</accession>
    <name>A0A5B0P613_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ER membrane protein SH3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002245</name>
        <name evidence="3" type="ORF">PGTUg99_012695</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000011">
        <property type="protein sequence ID" value="KAA1137187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P613"/>
    <dbReference type="OrthoDB" id="5229808at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013248">
        <property type="entry name" value="Psh3/Shr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228:SF1">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08229">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00786">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="30703" checksum="FA34ADA361E93ACA" modified="2019-11-13" version="1">MRLLYLIDQKEPEQPKTTPPSPCPSALPTLTVFDGVSNALLLSSIGFLLGALFMALTTDYALLYTHAPTTAKAYLAAEQAYLTLWTSPLADKKAIFHLLLLLPIFTLSVEVSRYTEAAIYFDGLCLGMMLLVLGLYTGSTVPNLRKLAFEPSTQALADLVKNSLNLKIPTDFDPLSPTSVINRLLVLLSHPASILLKPQKALESEQEFKKALELNPVDEKSRHELLAITAAGHSIAIFLLIGIIVLQIGKVYAEVEDAKLKARFELEESNRVAQDRKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7P0</accession>
    <name>A0A5B0P7P0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7P0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="120" mass="13306" checksum="B29A8132221478DD" modified="2019-11-13" version="1">MTDQAPPAGFLTSPHPILPSATTSLQAICIRDPAGPNAPNPTRRPTRPDPRAPETHSKQENPRTPDFKLIQTDPDRSDPIRSGTQKLRLFRYFSAERSETSLLAAVISALKFNEIFTKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PE76</accession>
    <name>A0A5B0PE76_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010281</name>
        <name evidence="3" type="ORF">PGTUg99_013118</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000348">
        <property type="protein sequence ID" value="KAA1098658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PE76"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="94" mass="10715" checksum="C99FDD84D52D8454" modified="2019-11-13" version="1">MARNYDIIDEYHQDTTSSVPDCQDTLLEVIKVLQNDIRKGGGKINRFKLHFLWTSTDGEINEHLVQEHASNLNETDDAENNETNSNSPSVQLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFJ0</accession>
    <name>A0A5B0PFJ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFJ0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="221" mass="24424" checksum="3E152393C5484F07" modified="2019-11-13" version="1">MGPEPTPISPLKDTWYIANIIIIAGVANSEVIGKKPDSSHGAKSGNFTKWSSTSSTNHSTRLGRYGKAADGLKPSDFYINTQLPISTFDPNASVSRCAALSPFGNNRLVRVDGDIPRSAFISPHSIRQNKHASAHDIEIYRKGHVIQPPATEGPRLHWRRLKKFFSILKTSEFPNPVFYVNLCCAITRAHRRPNNISRPVWHPSLPELSGDQDKCAFASPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHH5</accession>
    <name>A0A5B0PHH5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHH5"/>
    <dbReference type="OrthoDB" id="3205825at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179">
        <property type="entry name" value="PROTEIN CBG02620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179:SF2">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="380" mass="42917" checksum="A73769FD4A15AB67" modified="2019-11-13" version="1">MLHFHDLPKREMTSSINVPTLQNASDHDLTGVVFGFTLGFGFFVICHAVKRTRKISAFTIMTWLTILVTSVSAWMVFFNLENIVHRPLAYYVVLSILWAIQSQCVLQIIVSRIVLIWSNPDHIRRLRWGVFTWIPARIQISAAYTKFETFWEPSEKTLCLLTDAFLNVIFIISVRKTLISNGLTKYNELVKFNVFVIGLSIALDLGIVSSMFMHGSLPYESLNSLSGFVKLKTELLMNELIVKVIKSENQMKMLSDNAIGSSDSSSVLQGFQTDMYRKGSNFRKQEHNIQGTRERILSTSTISRPQLRMSLTAPPGRFYRPGSGSLADLESGHSSGIDLHSPGYTSQSNRLSSDKEWEIPSPAGSFELRRSNELLIPVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIM6</accession>
    <name>A0A5B0PIM6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIM6"/>
    <dbReference type="OrthoDB" id="2505640at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="268" mass="29262" checksum="2DB63391C7EDA363" modified="2019-11-13" version="1">MVGKIKRALIDSAPSSPSHPTHLEIEPCTCTQRRTSPTPLSNSPNSPKQQKMVAFAHLITLLSVLVAMSRADEVAQAKPEQVDDKWYYGRFRSGYWGLSGMSLYSTYPSLYTNYYSLLNSYCYGLGTFPYSYNLINGYFAKATDAEKSVSRRAISLDAEQLVRRDTADSFTCTTHGAAPQTFSAKECVAAAQQLSEKKVSTASHGGCKVALANAKEKVAPGHISSKDLETAVRSILSGCSNAENKEVANNKPEKNIDEKQVVMLITKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIR3</accession>
    <name>A0A5B0PIR3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIR3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022839621" description="No apical meristem-associated C-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24196" checksum="7CFB4F619F04FE35" modified="2019-11-13" version="1">MMIIGKIDLLHIALYTLGVSQLILSTPIEGFGDTSEIQRGRSGYQNSRIGEAGYSGAGRSRNGRSKGKGENHNQYIATASNSTITNLYLTTTASIDLEPERIEKNYKSQIETHQQLQNKYPDYDQHEAAKITSRLHQLLVDNSKNEVSTDKMGDEIDQISSEFGSWLVKIKANQITHPHPEFVQDFNNVYLKLIPQARQKLIERLRQENKFYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLE9</accession>
    <name>A0A5B0PLE9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023087</name>
        <name evidence="3" type="ORF">PGTUg99_027239</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000339">
        <property type="protein sequence ID" value="KAA1100789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLE9"/>
    <dbReference type="OrthoDB" id="2509996at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="352" mass="39927" checksum="890686A61B2C8A94" modified="2019-11-13" version="1">MMEEYIQTQQYEEPCDRCLSQRLYCVQSTAKRGDRGKCEHCRWARANCNATRLNKSAARWKPGLLWSNTLLKEVQSEIPYSSDNQPNPKKEMDSEEDRETMPDEPAGPSKKKNLRPSPPPETSPKRKRMISESEESEEEGGSSIENQKIAKKSRRKTYASGSSKKLPDTEVDREEGGSFTKKRKTKTSSRRKTCATELSKEVPEPGADHGLSGNVSATQVDDTNPGKDKGKESASTNTNETPRQDSEQPSENQNPNGTPAAKDTYEDWLTPDKEVREGSWISETDRSMEKKLEKVKEVEETLNRLEIELKQGVREISRLVGKQEKEKMKEEVRKIKESLEKSKVDLTAVITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMM4</accession>
    <name>A0A5B0PMM4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PMM4"/>
    <dbReference type="OrthoDB" id="2505701at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026768">
        <property type="entry name" value="YPEH2ZP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31841">
        <property type="entry name" value="PROTEIN FAM72A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31841:SF1">
        <property type="entry name" value="PROTEIN FAM72A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="120"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="391"/>
            <end position="563"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
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            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="439"/>
            <end position="457"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
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            <end position="467"/>
        </location>
    </feature>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
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            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="600" mass="66692" checksum="804B8FD9CBB656EF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
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    </protein>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </evidence>
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            <dbReference type="EMBL" id="KAA1103117.1"/>
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        <source>
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    </evidence>
    <sequence length="381" mass="41818" checksum="675D0E5B96F97F50" modified="2019-11-13" version="1">MPTVSKILCPETQYDEDTLIDNPPRKAPICLDFSLYVKSVNIPEFPHARWVDAGNGWVIISPTRELAVMPVDLQSMTWSHFQGLAIQHLGHEAPMARLLGGAHNAGALLWQAFILGNVQFSQKNKTVIRGPQEFREFADQAYHEFPNRVTFKIVMLQPPGNVVAKGAKESIDGNQAGKNDGLAKAPAPMRPRIRPTKSKLRLAPHNEQQLTEALPAKRGLVPEENGSAQAMDESKTAISNHVGEGETGPSDRPAKHAKKAKTPAAHDTDDIEVIRGPLAVRPPATPPANGHSGVYRVPLGPESPQLEAVDMETYLMVSHIKPTDQATRRRLRTHGIIHWTFFRATSEAELLELGFTLGIACLLCEGVPRLEAYVAERSVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
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                <person name="Sperschneider J."/>
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                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
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                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="320" mass="36137" checksum="10DEFB376261AEB1" modified="2019-11-13" version="1">MADSESSARSKTADLVVEALDCLVHRYHFSLNQIFGEQLKTLTIDDMEKKAGLLEELESSLLPSIKDQITALLLSLDLRGWGKPPRPDFELALGIVSDLDQTLYETIASIECISLESPLPAETHDHHLRSCKEFRCSRLLWRIKAVVEGHLCELFRNYIDFIRLWELSSSHPDSSEHHVKTSQKEAEILKITARSGHLIDAIISLFRKSDLDFIQEEWLAAADSLNGVLGFLIILANCPKEPNGINAGRDDHRTNGNRAEIAEVARLAIPLVKLTRAFLDKIGNKTTRKAAFTLLEFNSLSNPSKEESIRIRLFDKRTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0PP99_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        </lineage>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
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                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
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        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="60" mass="6681" checksum="3B6B2E72433558E3" modified="2019-11-13" version="1">MSLPIRWTTLPTRFSLLSLCFLGLALKINCFPADGLSTLPEDEFSVYQQARSGMSQTDVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQY2</accession>
    <name>A0A5B0PQY2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003331</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1102990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10445961at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
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        <location>
            <begin position="26"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="552" mass="54480" checksum="F3BF8B002EF158FF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSB3</accession>
    <name>A0A5B0PSB3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle subunit SRP14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Signal recognition particle 14 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_020151</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a fungal signal recognition particle (SRP) complex that consists of a 7SL RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SRP14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSB3"/>
    <dbReference type="OrthoDB" id="19209at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.10">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, srp9/1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003210">
        <property type="entry name" value="Signal_recog_particle_SRP14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009018">
        <property type="entry name" value="Signal_recog_particle_SRP9/14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12013">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02290">
        <property type="entry name" value="SRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54762">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, SRP9/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1103510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="157" mass="17740" checksum="1D467228A94E99AD" modified="2019-11-13" version="1">MSLSNNEFLAKLTELFTPDPQRPRTCFLTQKRFAYQADGDAIMETPGKEPTFKCLFRATDGHAIKFSTLVEPDQTLVFHKAYSNVMRSRMIAKLKPKKKKAGKPAAVSAVRLPKIVGPKRGPGVERRRRLVAQRSKILMKIRRRKLLKVAATNTTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZP1</accession>
    <name>A0A5B0PZP1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-dehydrosphinganine reductase</fullName>
            <ecNumber evidence="9">1.1.1.102</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TSC10_1</name>
        <name evidence="15" type="synonym">TSC10_2</name>
        <name evidence="13" type="ORF">PGT21_034191</name>
        <name evidence="15" type="ORF">PGT21_035389</name>
        <name evidence="14" type="ORF">PGTUg99_033397</name>
    </gene>
    <organism evidence="13 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 17">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reduction of 3'-oxosphinganine (3-ketodihydrosphingosine/KDS) to sphinganine (dihydrosphingosine/DHS), the second step of de novo sphingolipid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sphinganine + NADP(+) = 3-oxosphinganine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.102"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:22642"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.102" evidence="9"/>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZP1"/>
    <dbReference type="OrthoDB" id="10267115at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047560">
        <property type="term" value="F:3-dehydrosphinganine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006666">
        <property type="term" value="P:3-keto-sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08939">
        <property type="entry name" value="KDSR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000468">
        <property type="entry name" value="Short-chain dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045022">
        <property type="entry name" value="KDSR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550:SF3">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1106385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1109438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1115342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="314" mass="34485" checksum="7A32CCB6567E9DCE" modified="2019-11-13" version="1">MLQFRKNRFNPKGQHCYIGGGSQGLGLALGCLLAARGAHVTIVSRNQKKLDEALEKIETFRTSPSQKFASLAYDLSSVDGAEKAMDAAAEPFGGKVPDHVFSCTGGAAGILGYFIELTPDQLKQSVDVNYWTAVWTARAAARRMAKQGVRGSIVLTSSVLGFFAVPGYSAYTAVKHAIRGLAESLRIEFLLYNINVHCYFPATIYSPGFEEEQKSKPELTKIIEGADEGLTPEQCALRLVRGIEKGHVMITSDPIGHLFRNSMRGMSPVSNYILDPIFAAVAPIAIPIWRKITEYQIRSHIPQHKKEVIDRLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1R1</accession>
    <name>A0A5B0Q1R1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1R1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="163" mass="17968" checksum="4C76891D18257F5B" modified="2019-11-13" version="1">MDLIPGIGIGSLHPIQSQCIGQLGLGGAIRYRYLVLVWMIPKCKAYSPQHLGAGSGLQCNPDPDPIPWDWITLSNPIPYFLIPGIEKLIRSDKLADTVLDWICFTVEPSLQISFESHEKLSFLEAKKAQGLQINSPPSNLKPKFNPTPFSTPKTSSPIPDPCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q389</accession>
    <name>A0A5B0Q389_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_050135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q389"/>
    <dbReference type="OrthoDB" id="2509965at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="549" mass="63114" checksum="B22F8ACF9361327C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q443</accession>
    <name>A0A5B0Q443_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MDM20/NAA25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q443"/>
    <dbReference type="OrthoDB" id="1874341at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019183">
        <property type="entry name" value="NAA25_NatB_aux_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF3">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 25, NATB AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09797">
        <property type="entry name" value="NatB_MDM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="912" mass="103434" checksum="87EF164E523E1B4D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5Y4</accession>
    <name>A0A5B0Q5Y4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5Y4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022775057" evidence="1">
        <location>
            <begin position="24"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="88" mass="9620" checksum="0284126E098A6013" modified="2019-11-13" version="1">MGVFLNVLIAFLVLLQGAVMVSSIPVNLEENAKSLPRYVPDPDPKGAGEVIFRGVSSPRTRFFQATAVSSMGLYSFPSEFQAQYDHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6V7</accession>
    <name>A0A5B0Q6V7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6V7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022934221" evidence="2">
        <location>
            <begin position="21"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="141" mass="15306" checksum="7471C68BCD589F63" modified="2019-11-13" version="1">MKHSLIGGIVVLLHLEFLSGTIRRNLELFSDLDLSLGNEQVSKNKLKTLRLFDDSADEGGLEGVDKSRNNPSALVQPSCEIGHGGAFPPFAKRPKLDLSLSLGLPAWCQDEITQTGSSSPRSPKRNPSGSNQPVLMRNETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7G5</accession>
    <name>A0A5B0Q7G5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative PHD type zinc finger protein with BAH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SNT2</name>
        <name evidence="7" type="ORF">PGT21_030718</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="336088at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048189">
        <property type="term" value="C:Lid2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036205">
        <property type="term" value="P:histone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15571">
        <property type="entry name" value="ePHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029617">
        <property type="entry name" value="Snt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="5">
        <location>
            <begin position="17"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="787"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="257"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="355"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="631"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1020"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1189"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1257"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1399"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="355"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="659"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="686"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1041"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1073"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1137"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1189"/>
            <end position="1199"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1213"/>
            <end position="1232"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1335"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1403"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1474"/>
            <end position="1486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1109009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1496" mass="163587" checksum="981CAB7E2EA21F1E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAW3</accession>
    <name>A0A5B0QAW3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Galactokinase</fullName>
            <ecNumber evidence="3">2.7.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Galactose kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GAL1_3</name>
        <name evidence="16" type="ORF">PGT21_011081</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>alpha-D-galactose + ATP = alpha-D-galactose 1-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13553"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28061"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13554"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. GalK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.6" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAW3"/>
    <dbReference type="OrthoDB" id="187738at2759"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.340:FF:000003">
        <property type="entry name" value="GAL1p Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00131">
        <property type="entry name" value="gal_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF7">
        <property type="entry name" value="GALACTOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457">
        <property type="entry name" value="MEVALONATE KINASE/GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Galactokinase N-terminal" evidence="15">
        <location>
            <begin position="35"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="13">
        <location>
            <begin position="152"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="14">
        <location>
            <begin position="416"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1110114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="569" mass="62174" checksum="19CC97A26A7DC16F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBC8</accession>
    <name>A0A5B0QBC8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBC8"/>
    <dbReference type="OrthoDB" id="2500774at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="530" mass="58601" checksum="0B2AE885FE8ED367" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QE61</accession>
    <name>A0A5B0QE61_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_003063</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QE61"/>
    <dbReference type="OrthoDB" id="3026777at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF1">
        <property type="entry name" value="FI18259P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1111526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="53844" checksum="BF4B98E309ECCCD3" modified="2019-11-13" version="1">MTIESITLGCFSALSTGYWSRLSEKRGRPNILLIVYLGLGLSELIFILVKVFKLPVNLLLIGSLVEGILGGPATLNALITGYVHDHLSQHQLSTATGLMLSAGAAMVGSAIGPSFANWLIAFSDAQLLAPSYAILVCLPPLALVTRFLLPHKKLSVSMDFSGDSQIQRKHILAPLHVLRPVRGDRSLLLLGISYGLYCFVGGSSTVKLQYAASEFDWDAVKIGEYLTILGSSRVASLLIVIPLILYVWNRKANHDPVKPSSVDGSDLLEGQNVAGDFHYASMDLNFSNLSPHSKPTAVVFGCPSTNTSRSSLTLCPSEVVLEVNEDDTRCKTSATKDQELELNSSISQKSYQTQIDLNLARFSIGWGMICGVCLLFSHSMLSFIVPSALEALSRSTGPAIQAVALSLIAIKRDNIGSADVISSFSLIGILAYDLFGPLLFGAVYVTSRSQELSKCFLFVIVALEIINLTVISFVDISEKKAVDEEFYCSEGGQVLLPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QF06</accession>
    <name>A0A5B0QF06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012643</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QF06"/>
    <dbReference type="OrthoDB" id="2496574at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022766464" evidence="2">
        <location>
            <begin position="21"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1111800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="560" mass="64872" checksum="0A3EC0DC40D1FF25" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFE8</accession>
    <name>A0A5B0QFE8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFE8"/>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="32307" checksum="682BAED8CA83B292" modified="2019-11-13" version="1">MEKQGFVTNREPHLKKPMDVPQSVRAESVSVSSVSEVKHPQSAGLLKPAPSGTHNLDKDFTKSIELARTETILAEVSLENAVILYLSPGWTKVTGLDPQSVIGTPLGELIDGNYELFNEANRRLMEDVGNTVELQFTIKISLASSDQEGDSSRSITTRDHFLPMTAKGMLMVDRITGEGVHSMWVIRLNSHKKAPVSTGTTMETKGSHHTRSHSDPVAQFPAPAPLSTEPLLCRICEHYLPAYYFERHNETCAETHRLEMQVSECNDRLTDLRDIINELRGALDRNGEYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFQ8</accession>
    <name>A0A5B0QFQ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFQ8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="67" mass="7444" checksum="77B4429104C33BBB" modified="2019-11-13" version="1">MLLVSAGSIVVVIIGTLENQIEALEAKQKEHMVKLEAQQIELEAQLEAQLEVQQIELEAQLEAYQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QG36</accession>
    <name>A0A5B0QG36_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QG36"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="162" mass="17928" checksum="DB0BFB1658042C39" modified="2019-11-13" version="1">MAGLRRVRYVTLDASPSFGGRYATSSEGPCVKFGDLRRRDLVLSSVRDGLPQAHRNPDQRESREASQMAGQWVNAVSNDPIHHDVLDQIALRRVQCKAYNVYIRGVPLPLAGGARRACLSLQPRFLQSCLQAMGLRSIGEPRLLGGTGTPKCKLDVRRVDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHL9</accession>
    <name>A0A5B0QHL9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHL9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="200" mass="22588" checksum="B15D3912A63C27D7" modified="2019-11-13" version="1">MQGIGECEYKQAPLEVSDTFSLYAAEAAAAARLCLEDLTEAQQIFGVDEITKDGPESSINHPFVHFLSSFFHLCTVQIILVQTNRTFLYEIPSFPIVNSRTIPLRSPYNNEHPVVGAGYANLKKLVVIVDKRIGVPSMERIGYPCLYNCEACRCPCHLNEPPHPSLTFSLLLGDLHHHHHHHHHHHHHQVDVLDPPQQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHM7</accession>
    <name>A0A5B0QHM7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHM7"/>
    <dbReference type="OrthoDB" id="2516525at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="865" mass="97283" checksum="7A3995C0E78D304C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIY0</accession>
    <name>A0A5B0QIY0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_026985</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIY0"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046972">
        <property type="term" value="F:histone H4K16 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000159">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF219">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="15">
        <location>
            <begin position="161"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="73"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="555"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="13">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1113271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="615" mass="68616" checksum="45C159359C257A66" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMZ7</accession>
    <name>A0A5B0QMZ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMZ7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="82" mass="8364" checksum="712A67D85E151900" modified="2019-11-13" version="1">MRQWRGHSHANAAHSRELGLGSVRASLTVLLMRDLGPTSAGRKPFLASSGHAEQAADGSGGGPKLPSAATFGSFGIPSSKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNJ5</accession>
    <name>A0A5B0QNJ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNJ5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="104" mass="12054" checksum="27F112C5F1A60E27" modified="2019-11-13" version="1">MKGTSPSRPPDPPPTTTLFFQWIVWPSFFFKAEKQQLKKSFDENVKENKENHQNKVDREKEELLICAETLKQATNDCIKTTLRNQPFTPTINFADALGCKEGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNZ0</accession>
    <name>A0A5B0QNZ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peroxisome chaperone and import receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PEX19_2</name>
        <name evidence="2" type="ORF">PGT21_025872</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNZ0"/>
    <dbReference type="OrthoDB" id="21292at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033328">
        <property type="term" value="F:peroxisome membrane targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045046">
        <property type="term" value="P:protein import into peroxisome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.900">
        <property type="entry name" value="Pex19, mPTS binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006708">
        <property type="entry name" value="Pex19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038322">
        <property type="entry name" value="Pex19_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774:SF2">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04614">
        <property type="entry name" value="Pex19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="321" mass="35766" checksum="FD643857BFEA4C91" modified="2019-11-13" version="1">MSTEKTMINGVSIEDDMEDDFDDLLDKFKAPTETKKEEPSLPKEKASEGAKPDSSGTEEWPNQEISDEFARDLAEGMEALLAGMKDEDGGEFKKHLEALVSADGGGLKGATDLLEALNQNDPNPNQQSSSSSSNLPEHESKNFQEAIQETMEKLKRSDDSAKAKSGPSGAGEEDEKLSALFEKMMSGENGLPDETQLQTILQSFMEELMSKEIMYEPLKDMNRLFPDWIEENRSKLSPEDMKRYETQAKIIEQVIERFDDPSWDSEEKAGGDRFLSRQKEVIELLTKMQDCGPPPKEILGDMPPGVFGEDGLPNPEQCLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ17</accession>
    <name>A0A5B0QQ17_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_029266</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ17"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022827070" evidence="3">
        <location>
            <begin position="28"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1115005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="380" mass="41804" checksum="A9EFF3A438184FE4" modified="2019-11-13" version="1">MRPSMSIFLEYYRIVLVALCFFQATQSIPPFPHGIGKHADAEDFDSSFIALKMFGSSEGMGWTTNKIPNLEGRVAIVTGANSGIGYFTALELARHGAKTYLACRNPGLAEEAINKIRAIVPGSDLRFLKLDITSLNSAHSAAQEFIKSEERLDILVNNAAIAFKPYELSEDGIELQLCNAIGHFAFTIPLLDLLKKTSKDPESHVRIVNVSSVAHSWVLGTPDFSSVENLNKHSYCPIDRYGLSKLMNILFTNELQRRLSNTNVICASTYPGGVNTKLIAGISVKNYPILKNLGWLTSRTLLTPKDGAITSLYAATALEVEEKGLRSAYFCPFGVAAKPSKLAQDRDLAKKFWELCEKLVKEKVKSLSPAHLKISIHPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQS6</accession>
    <name>A0A5B0QQS6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQS6"/>
    <dbReference type="OrthoDB" id="2507622at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022664191" evidence="2">
        <location>
            <begin position="23"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="314" mass="31103" checksum="8614E464AFECEBBA" modified="2019-11-13" version="1">MRAFHLSKVVFGFLLAFQTSIANDEAPSVAVSGSVNSCGPVPLDIKGGTPPYTITIKKADNPGGPPLRSVPDANKTPSLSLPAGMTNGMVITFEVKDSKGLTTTSGQSTVSPSTDCPQPPQGPAGKPDGAADAKSPGDVAPPGPPGGDGGAKNPTDGSAPASPENPPGGSTPAPAPSPNPNPAPPAPGQPANALNATGNSTATGPQPGDPPSSNTTNISGSINANMTDKAGSGVNATMSDNSASNNSTGKAGSTNSTTITPPRPNYTPPKNNGNSSSSVNNRPTPPRSGASRGQLNSFYLHLFVVCLTGLIISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QW00</accession>
    <name>A0A5B0QW00_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Serine/threonine-protein kinase 17A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">STK17A_1</name>
        <name evidence="13" type="ORF">PGT21_006816</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QW00"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001194">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1D, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="125"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="56"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="434"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="202"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="251"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1117418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="489" mass="54566" checksum="321FD0F1EA736D4F" modified="2019-11-13" version="1">MVGIRNSFILNCCTLSSIGQPSRTEEPLDPPTAHTHTHHITSQGIILKGGQGYYYLHTGTSPPQQKPLPRKQEHHQYTSKKKQQKETQSQRKMPDSALASLHPKRRVLRALGLRDPNLHARKSEFKWGAHLGSGTYGEVTQCTWLTKNPPQEVAIKVVAKSSVPNLQQHISLMNRTLKLHHPHIVQMYDWFQSKEHFYMVLELATGGELFDHTITRGNFNDREACEMIHQVLDALSFIHSQGIVHRDLKPENLFVRGSAPYVPPYDLVVADFGVAVYVDRPDGSTASLTGICGSPGYTAPEVYRRLPYGKPVDIWATGVIAFILLSGRFPFAHLSGQAFLDDSDAEIKFPKRFAISPQAQNFILGCLQTEPSKRWTAEEALEHPWFDQLEEPIEEDVGERQTAASVADTDHTRCEQLPLVRADEPDDKQVESLATTAASSAERVGSALPPPSSHLSTPRSDDDPEPQLVLVHRSHTVNEDPPLVVDIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX91</accession>
    <name>A0A5B0QX91_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QX91"/>
    <dbReference type="OrthoDB" id="10285433at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="405" mass="46204" checksum="F011B499FFC94145" modified="2019-11-13" version="1">MEDQSSDTSDEVGPQIRHLGDLVIQAFRNLKHIYESQQGTNGEARLTQATVTARASPKEIHYDRLHSSLLPLLKELLTTITNLLFPGTLRMEAESRFRKVLELLPELDFQVSQVDSAVRMICPGTLSLESQTDDHDLKQFKRFRLIGLKEMSGQLLSEICVFLHSASEYVETMDLSSGGLFEEYDDENNPQAESDQAFYTADRMIKHLNRSELDVAEGCWAGALDALDELFKNTFSIVTPNAPETSPLRRKLVRQPVIQLTQLTLPIIKLIKLFFKKLSKDGMNHKRRLKLPSFTQMNSHQLDSLSKSAGKIAVELNEIVLLLTQADLAIAEWHRIHFQSIIKIAERLPTHFEPALLSVVLYLIPLVAPPSDQKYYQTWFVTWNILINTALHNFRRLARSLNEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYT3</accession>
    <name>A0A5B0QYT3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027538</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2499131at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022674347" evidence="2">
        <location>
            <begin position="27"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="725"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="328"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="406"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="784"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="851"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1281" mass="143112" checksum="B92C7D2733CF46BD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZ88</accession>
    <name>A0A5B0QZ88_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032423</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZ88"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1118145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="96" mass="10310" checksum="34108B5EAC37EE4B" modified="2019-11-13" version="1">MSDGVGGTIKTCAVDKAEICTYVVIESIISADSGTQAWHWAGRGPDSEGPTITRITSLGERGCAMDSQHISCQAKKGLLLETGHNCHVRYLEHHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R119</accession>
    <name>A0A5B0R119_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018909</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R119"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="266" mass="29965" checksum="B1C4DE16DB765A96" modified="2019-11-13" version="1">MDFVRKGDPYRDLHNWWNLHSVETPGDSLVNLDHASASPTTAVQILIQIQMNCDPQVGTLIISTLSCIKAGVLLTSLVDLTGYSSRNPPATQSSSTTRTTNFNNSFQPSLTQSTTSITLFTLDLVKLLQHITVSYPFVVFYIIFLIVVLKARRMTDNLNTSAASKSTNTNQASLTKTTDSKPDMDKINAMILKTAIKAIPMLTQDNYSMWNSRMMNFLELQKLKDTFLKEDEENLTGDDELQARTILTSKLDPSMINFKQLEHYYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1X0</accession>
    <name>A0A5B0R1X0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1X0"/>
    <dbReference type="OrthoDB" id="2500831at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="769"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="814"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="769"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="814"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="841"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="888" mass="101928" checksum="C486032202A5B13C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R383</accession>
    <name>A0A5B0R383_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_023924</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA1 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R383"/>
    <dbReference type="OrthoDB" id="1920692at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017384">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17098:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17098">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MWFE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15879">
        <property type="entry name" value="MWFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1119384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="83" mass="9474" checksum="27761D05ADACEA1E" modified="2019-11-13" version="1">MPWETFIPLGLLTVMFGASGTLLNAARRFQNDGKPPDYTVDKWDEMMQERNRRLTGSLRGQCVEEKAPLEFQTSSAWPVEKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RF12</accession>
    <name>A0A5B0RF12_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023558</name>
        <name evidence="3" type="ORF">PGTUg99_022349</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000208">
        <property type="protein sequence ID" value="KAA1123505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RF12"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138120" evidence="1">
        <location>
            <begin position="25"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="111" mass="12505" checksum="EA16C7745468C2DC" modified="2019-11-13" version="1">MQTHTLNLCLALVGALILYPLVSAHFQPYKARHCVQCKDVSQVETLQPIEKKHVTCWNKTPCQFALLTAFYHCKVCNTSVQAPLEKRGNGHVLSEIYITEPPVNKFDSHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RG26</accession>
    <name>A0A5B0RG26_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010644</name>
        <name evidence="2" type="ORF">PGTUg99_035977</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RG26"/>
    <dbReference type="OrthoDB" id="2506774at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1124851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="105" mass="11384" checksum="84FC84F0145637E5" modified="2019-11-13" version="1">MVEANIQLAAALHEGLAQTDNIPKRQRLQTRQMELDVQIREVEIQRTRATLQQEQATGAAFACAKMLQDFIQSGLPLADAVTITDQLLGPVGTGINVVDGSVDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJC0</accession>
    <name>A0A5B0RJC0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="3">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">DCW1_5</name>
        <name evidence="8" type="synonym">DCW1_1</name>
        <name evidence="8" type="ORF">PGT21_017689</name>
        <name evidence="9" type="ORF">PGTUg99_004348</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000177">
        <property type="protein sequence ID" value="KAA1125479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJC0"/>
    <dbReference type="OrthoDB" id="9984024at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF36">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1090910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1125479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="331" mass="36356" checksum="65CA53172DEEAE33" modified="2019-11-13" version="1">MHSIHFQVIAGAELFGPKAQMAGGNGDWISLATKTYDQVWAQADYKCGGGIYWSRNRQSKGGNYKSFITQVEFISQGARNFIQTKNATAFEQSKIILDWTTTSGIANQQSGVIWDGVEADACGKFSTSLWSYNYGQMLGSLAWMYKATGEKKYLDMATPYFDYGARTFAGSNTSGIITEQCEADKTCNRDQQGFKAVYVRNLAYLYRITNNQTMKAQIQNMIDTSVKAMVDKSCDQAWNCGGNWTQDTQPIKYVRSQHVSAALLVAAIGVHGNQTNAVFSNQASTFNSTGSGRAAVQNAKSAAQAVVFSSCKWILPQSLLVILLSQFIFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RXG7</accession>
    <name>A0A5B0RXG7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1,4-alpha-glucan-branching enzyme</fullName>
            <ecNumber evidence="4">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glycogen-branching enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GLC3_1</name>
        <name evidence="12" type="synonym">GLC3_12</name>
        <name evidence="12" type="ORF">PGT21_035184</name>
        <name evidence="13" type="ORF">PGTUg99_000310</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Glycogen-branching enzyme participates in the glycogen biosynthetic process along with glycogenin and glycogen synthase. Generates alpha-1,6-glucosidic branches from alpha-1,4-linked glucose chains, to increase solubility of the glycogen polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000112">
        <property type="protein sequence ID" value="KAA1130122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RXG7"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02854">
        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000001">
        <property type="entry name" value="1,4-alpha-glucan branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000080">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000250">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="11">
        <location>
            <begin position="160"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1081399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1130122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="679" mass="78064" checksum="1D490DF1DDF61828" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-02-26" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>B0B0H7</accession>
    <name>B0B0H7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Carbohydrate esterase 4 protein</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="11">Putative chitin deacetylase</fullName>
            <ecNumber evidence="11">3.5.1.41</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cda1</name>
        <name evidence="15" type="synonym">D25_2</name>
        <name evidence="12" type="synonym">D25_3</name>
        <name evidence="13" type="synonym">D25_4</name>
        <name evidence="14" type="ORF">PGT21_026425</name>
        <name evidence="13" type="ORF">PGT21_027999</name>
        <name evidence="15" type="ORF">PGTUg99_013451</name>
        <name evidence="12" type="ORF">PGTUg99_014684</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Biochemical and molecular characterization of chitin deacetylase from Puccinia graminis f. sp. tritici.</title>
            <authorList>
                <person name="Jaszczuk B.K."/>
                <person name="El Gueddari N."/>
                <person name="Fehser S."/>
                <person name="Moerschbacher B.M."/>
                <person name="Fischer R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12 16 17" key="2">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="12 17">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.5.1.41" evidence="11"/>
    <dbReference type="EMBL" id="AM260196">
        <property type="protein sequence ID" value="CAJ90973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000105">
        <property type="protein sequence ID" value="KAA1130944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B0B0H7"/>
    <dbReference type="SMR" id="B0B0H7"/>
    <dbReference type="HOGENOM" id="CLU_021264_11_2_1"/>
    <dbReference type="OMA" id="WKVELAM"/>
    <dbReference type="OrthoDB" id="2125469at2759"/>
    <dbReference type="BRENDA" id="3.5.1.41">
        <property type="organism ID" value="13098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004099">
        <property type="term" value="F:chitin deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10951">
        <property type="entry name" value="CE4_ClCDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471:SF2">
        <property type="entry name" value="CHITIN DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033970970" evidence="9">
        <location>
            <begin position="23"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="10">
        <location>
            <begin position="44"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAJ90973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1068397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1069550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1104540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1130944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="269" mass="29850" checksum="A5CBB83F0F11565C" modified="2008-02-26" version="1">MNFVLQLSVLLLTIISQSFVRSSPFTTRATEVIPDPATTCSRPGLAALTYDDGPYDYENKISDYLNARHIKGTFYVNGNNYDCIYDEAIVKHLKRTFSQGHLIGSHTWSHANISSLSAAELNQQLDLVEVALIKILGVKPKFFRPPYGAFDQKSLAILKKRGYIVANWSFDSEDAVGATPEQSMASYKELSKQFPASQITLNHETYQTTAEKVTPYAVPLLQKAGYKLVHISECLGTGTKLTDLYQWVGKPSVRDASWTCNGTPAPTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJK6</accession>
    <name>A0A5B0LJK6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJK6"/>
    <dbReference type="OrthoDB" id="2502964at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="385" mass="42261" checksum="25250F7F20AC4234" modified="2019-11-13" version="1">MGASLVARVEGIGVDAFQLVTDRLLRDHQALPRERWLAVIRSFRQPLPPIEHATKPPDRQDDALDDRNKGKKQRTMYCLRHSGKPGQLVALIEDAEAPIRSHEDRTSQQPDLSSPFDSNEFHKKTEDNPPSSQNHEKHTGDVNTADSQPTPVDQPSTNHNGTETAAPHIAVTTEQESADMVLDGAIKTPSVKIAPSHYRVSIVSVPDSFEQLLSSTICAPQTSLIPSAWCPRPNIVAIEGMSWGIYSSSFESEISPTSNMGSMASSMWTKDTQPDWWIRLGTVTNKGATSGNTSLSWLILELECAASPIELDPSFELLESVMHALLRPDGKLLVKPFRPTARSLMEAGLTSEESNLATSSPHPPKTATLRNGYCMLMLAKQESLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKY6</accession>
    <name>A0A5B0LKY6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vesicle trafficking between the ER and Golgi</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SLY1_1</name>
        <name evidence="3" type="ORF">PGT21_002488</name>
        <name evidence="4" type="ORF">PGTUg99_013491</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1079958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKY6"/>
    <dbReference type="OMA" id="VNDLRAW"/>
    <dbReference type="OrthoDB" id="10251230at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1079958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="733" mass="80629" checksum="8AECC8C4821022B7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPI8</accession>
    <name>A0A5B0LPI8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPI8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="15814" checksum="122C4B81D814E3C8" modified="2019-11-13" version="1">MERELTLLNIRSSSLKTLMKPFLKYPVAIFVFDLAPAALGLPESHIGRSAPSSFPSFHFSQLTLIRSISLSAEVFFSFLLGFSVKTSILVYRQNKIIRDISYRGRKLSLLISGCFETHKISRPTISSLEKYESTHRKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPK6</accession>
    <name>A0A5B0LPK6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_009165</name>
        <name evidence="11" type="ORF">PGTUg99_004266</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sideroflexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000018">
        <property type="protein sequence ID" value="KAA1136876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPK6"/>
    <dbReference type="OrthoDB" id="6608471at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00798">
        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF6">
        <property type="entry name" value="SIDEROFLEXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1065750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1136876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="343" mass="37528" checksum="CD0D638480159412" modified="2019-11-13" version="1">MESKTTALEPIDIKKSRFDLDTYYGRLRHFITVTSPLTLFNSTDEIRRAQELLKDYAAGRRADLDGSHETQEKVWAAKQVVEASLHPDTQEPIPLPFRMSAFVPTNLIIATGLLLPNPSLASIIGWQWANQTLNVCVNYSNANKSTAMSTSEVAKAYMSATVTSVGLAVGLNRLVPRLAKRVGHDTGLLLARFVPFVAVASAGCVNVGLMRWKELRDGIDVYPPGVSDPEQSVGKSRIAGSYAVGQTAASRVLTNIPTLILPPLIITALQKRGTFSGPRGKSLEMIANLGLIGGSLFFFLPPAIAAFPQRAKIQAHKLEDRFSNIIDERTGKPYQYFEFNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQF1</accession>
    <name>A0A5B0LQF1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Pre-mRNA-splicing factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CWC2_1</name>
        <name evidence="17" type="ORF">PGT21_011138</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CWC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQF1"/>
    <dbReference type="OrthoDB" id="10251848at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12360">
        <property type="entry name" value="RRM_cwf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000502">
        <property type="entry name" value="Pre-mRNA-splicing factor cwc2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034181">
        <property type="entry name" value="Cwc2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032297">
        <property type="entry name" value="Torus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16131">
        <property type="entry name" value="Torus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="16">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="156"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="13">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1065818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="227" mass="25877" checksum="5634443906702DB1" modified="2019-11-13" version="1">MAHIPQQPTEKQITVADQPVRKKVKRRPARKQVTLEQAAAVKKQPEQNGQTYNVWYNKWAGGDRFDSSTQKEKSQTRCRIATDAGYTRADGGNNAYCCLYFARGCCPYGSECSFLHRLPPAQSTLPDASLDVFGREKHSDYRDDMGGVGSFSRQNRTLYIGRLREINSKTEELVETHFQEWGEIERIRVLHGRGVAFVTYLSELNAQFAKEAMMCQSMVDEEVLNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LR61</accession>
    <name>A0A5B0LR61_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000194">
        <property type="protein sequence ID" value="KAA1066835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LR61"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="294" mass="30844" checksum="E3EF650C35D5C1B0" modified="2019-11-13" version="1">MEQSRASHRPAQSSNRCEDETDADEADNDADETDDGDDKTDDDADSQAPTHATRSATWEPSSPEAPSSHLTHDLLKTGTLKGAASESLSNEPPTPSVKDESDDDADSRAPSHATRSATWEPSSPEAHHQSPIAASSEAGDSERTASQPPSAASDSRPAEDGHSEGAASESLSNEPPTPSVKDESDDDAQTLGRRPMPLVRQHGSLPPLKPIISPHLPPSSERTASQPPSEACDSRSERTASQLPSEASDSRSERTASQPPSEASDSRSEQTASQPPSEASDSRPAEAASEGPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRG6</accession>
    <name>A0A5B0LRG6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_011979</name>
        <name evidence="3" type="ORF">PGTUg99_017900</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1066945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRG6"/>
    <dbReference type="OrthoDB" id="2503634at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="132"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1066945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1083936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="225" mass="24761" checksum="D5225F21269FA87C" modified="2019-11-13" version="1">MSSGTVGIHSSHIMFHRAQRDYERLQQTYNQWLKKREKTKQDILKQITTINEAGHDPKKSTKLLSKLNAQLSAFEEEEAEVADAELLQHSPHARPCHRPAYRKLAFENALKQSEEKKRLAEEVYNAALAARQSAAGAEAAPSQPAEPAEAAEAPDEAAAPLSPETGTSSAPKPMSRLKKIDAFLTRLESGKGMTKTFAPDRQSPSYGKWSLRAGTFQPGRAKPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSY9</accession>
    <name>A0A5B0LSY9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YOP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">YOP1_2</name>
        <name evidence="8" type="synonym">YOP1_1</name>
        <name evidence="8" type="ORF">PGT21_022036</name>
        <name evidence="7" type="ORF">PGTUg99_011014</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSY9"/>
    <dbReference type="OrthoDB" id="10009287at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF161">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1066684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1071885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="157" mass="17620" checksum="56A76384039EB0AF" modified="2019-11-13" version="1">MTAYPRLESVGTVLSKYPALTKLEQQLQVPKAYGVLGLAGLFSIFIFFNIFAGFLTTALGWGLPAYFSIQALESPSSGDDVQWLTYWTVFGFFNIIETFADIILYWFPFYYTFKCVFIVWLMLPQTRGAQTVYHKALKPLVASTASKTSAPPETAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUP0</accession>
    <name>A0A5B0LUP0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUP0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="157" mass="17321" checksum="E6DCC58F10E23621" modified="2019-11-13" version="1">MTVHYDHGRPNGALMIAGKRSLQTISSTKLESMSFGVEVFHAYSGPQKKGTTHPRFACSIGYRLGKNLLACKNSQQPCSARVGCFWDRVFCAPPTKKKRKEKPTVIMGFVPSTLDHHQRRYEADTSTIAVPPLLPACQLSPLLPVGLIRSGSRMTVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUX4</accession>
    <name>A0A5B0LUX4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUX4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="346" mass="37357" checksum="4C90A26C97000114" modified="2019-11-13" version="1">MLTSGPPHRGSSICTCHWRLSEDRLGTPRSGFQPLLSQNNHQHQAHHCQIVVIASSAENRHCSPYLSPAHLAAKWVCPCPYSEGQQLTDVISSSANNQQATAPPPSSPQAPNNSNPSALADGIELVPPSTQPPLENERTWVHLSTSTRHPPSSPQAPILLLERLFLSGWPPTTRSLLPRPTESTISPPTPSCLWKTKQPGSTHRPRRRTPPPPFSPQAPNHLKPSASANGIDHLPPYTQLPLENETTRAPNHLKPSASADGIDHLPPFTQLPLENDTTRVDLSTSATHPPILSPGPQPPEAFCLGRRNRPSPPLHPAAFGKRHDPGRPIDLGDAPPLLSPGPQPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LV54</accession>
    <name>A0A5B0LV54_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LV54"/>
    <dbReference type="OrthoDB" id="2336871at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053216">
        <property type="entry name" value="Appressorial_penetr-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34587:SF2">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34587">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="451" mass="47855" checksum="DF0D92DE0D429BC0" modified="2019-11-13" version="1">MVYMTLHQLQATRKERVLNEDEDDPEGSWEASKQKGPKNQKKKNGCIRSSASINRGGVGGVSESSFGSHYRRPSSVDLGTHHLRVYKGFIKGEMATFPHMTRAPLAWPALVATIVLSVLVSERAAYAASTAKPPSPASAAAPEFVTKSLTSSDNFLHFCAGAKLTNGAQVHEGSCNPTPMGFIPSVENMPSVRIVEPSSEAVLPANQDFEVAIYAHKIQLGFFTPPASTYYQAPQQLNKKGLIKGHTHLVIQKSVAKRVFETQDVGGGIAFFKGVAEKQVNGIVKLTVKGGLPAGTYRISTLTSAMNHQPVLLPVARRGAVDDAIYIRVGGAGHSTPKSLVDGKALADASGAEPTKPVDNDGEAKEKSTKKGGKNKPVDHGADSSSDPQGEAGAAKEKDTKKRAKTNPADLDANKPAKGPPKDASSSSSSAQGEPPKQKDKDIKDVKNQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LV62</accession>
    <name>A0A5B0LV62_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LV62"/>
    <dbReference type="OrthoDB" id="3362250at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="304" mass="34539" checksum="AFA9E8058AAFC731" modified="2019-11-13" version="1">MASSSTSTSSEQTHTDYHQSSINYSITTFESELGKFCWEVKNPDGHLIFKSYKELTHDEIVEGIQDSSGETLWTIRRPIRGWYLVLRSPMDSNPQSFIALQPKKVTKGKNKRVEFTFRLPTLSHETTPDKPVGNGHLRKDSTDSSIKRRNPSVSSSPQSPNRLKPIHDQQAPSPTTEDLIHLQPVEEDVKDQTLFKSFTSLFTNSNQKFQCFAKPLQPTTINDQTVEIDPTILIDYEDQNSVFGIHNNGILKIRLKNSLNFKLIEGIDDVNQNEFHCAFWVAVCIAYLGFRADQDAYIAANEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1G6</accession>
    <name>A0A5B0M1G6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033538</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1G6"/>
    <dbReference type="OrthoDB" id="2503759at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022874253" evidence="2">
        <location>
            <begin position="23"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="539" mass="53313" checksum="44CF32983B77A2E9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M274</accession>
    <name>A0A5B0M274_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M274"/>
    <dbReference type="OrthoDB" id="2509951at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="371" mass="38796" checksum="17AF4B876DFDBB00" modified="2019-11-13" version="1">MKQTTLTSYPAFNRPGQEGNPLVIDSDDNTSNAVDCSSDIEFPNPRTSFKDAQQPSESGAIAQGSTRPSLSGCVGIQSQTLEKMEGTSMEPNPDASRNPTDPDFPASITDPTSKSSDSELSIPGIILKDSQQPSESEAIAQDSTRPSPTGCVGIQSKALEKLEGASMEPNPDASRNPTDPDFPASIIDPTSKSSDSELPIPGTILKDSQQPSKSEAIAQDSTRSSPSGCDQIQSKALEKLEDTVADPEIDTSSKSTDNSLLAKIGDPSIKTSCSELPSATKDAQQASGLDRVVDKCSLGFPTGPDGLTPKGPGNPEGMAPDTKCRSQTPDTVCLAKQQADQNTIVVKAPVSPVTNLLASGFRKALSAKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M671</accession>
    <name>A0A5B0M671_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030943</name>
        <name evidence="3" type="ORF">PGTUg99_001274</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M671"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000380">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF2">
        <property type="entry name" value="FE-REGULATED PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="190"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="562" mass="60957" checksum="C31D21BB300F35D5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6F2</accession>
    <name>A0A5B0M6F2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018384</name>
        <name evidence="4" type="ORF">PGTUg99_018609</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000174">
        <property type="protein sequence ID" value="KAA1125905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6F2"/>
    <dbReference type="OrthoDB" id="47172at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000056">
        <property type="entry name" value="Jumonji domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF94">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="296"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1125905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="481" mass="55067" checksum="2E4ACCC50A49C133" modified="2019-11-13" version="1">MVIACFRKSGRMGIHWKLTELWSRIRDFCDLEQCERSIQFQLKTTCDSLLKQFQDAISLEESQRACEETVATAEALLILSDEKLSSYVYKEVPTHWRQLYTDSILLKVSSIFACETNIPTSGRNPIDWMEMIRLLDMALIISGAPGRGRRSVIFFLIDSIQTNYIKPSGEAQDSGERPEKRRRKTLEDRPSGGSNPVSIPSVMNAIPVFTEAPSVEEFQKSSHKSPFVIKRYCKDWKALSTNPWESTDYLKAVAGPGRVVPVEVGKTYAEEDWSQTIMGWEDFLNKMYECDPDKLVYLAQYNLFNQFPKLKDDIQLPEYVYCDLPPDDRPKPDVEDGVILNVWLGPAGTVSPAHVDPYYNCYAQVVGRKYVWVAAPKFSTEMYPFGTPTSDQESGRPEEEIQDRNTLQANYMTNTSQVDVFGSVDQDRLAEFRGRFPHFVDKVMPEALQIVLEEGDMLVMPPGWWHSMKSLEPSINLSMWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6F8</accession>
    <name>A0A5B0M6F8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011931</name>
        <name evidence="4" type="ORF">PGT21_012516</name>
        <name evidence="2" type="ORF">PGTUg99_026130</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6F8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1077585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="90" mass="8145" checksum="92838191AC743CCC" modified="2019-11-13" version="1">MKYGGKPASDAQAFIVGKLKAVKAGGSPGGAAGGNTNGKGGADSGGGAAGKSMGSKPGGDMGGGKMGGLGGKLGGLGARSLAWHAASRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8G1</accession>
    <name>A0A5B0M8G1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8G1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11617" checksum="3883CBA4FE66FC96" modified="2019-11-13" version="1">MSKHASAQKWPGLDDWLKALSRSGFRELHSPLSLFLLKPSTFPLLSVSSHPTRISEIVSRSSIRPRCLHPRRGFSFLEQGLVHSGKFPSGHSPYLRTMKAYGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9D2</accession>
    <name>A0A5B0M9D2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">General negative regulator of transcription subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NOT5</name>
        <name evidence="14" type="ORF">PGT21_008046</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9D2"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012270">
        <property type="entry name" value="CCR4-NOT_su3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005290">
        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="12">
        <location>
            <begin position="3"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="13">
        <location>
            <begin position="593"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="339"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="339"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="433"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="473"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="536"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1073312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="721" mass="79821" checksum="1B138B7F3A2F92CB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MC09</accession>
    <name>A0A5B0MC09_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear mRNA export, poly(A)+RNA binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MEX67_5</name>
        <name evidence="3" type="ORF">PGT21_014957</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MC09"/>
    <dbReference type="OrthoDB" id="2510873at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="121" mass="13526" checksum="ADB5816285F005BE" modified="2019-11-13" version="1">MRGVIGDDCRNRPATPNRSQLMAKPLDTLPPQPPLPMSIQPAFMNDSSTSTFVAAFCLQFFNAFNHDCARLMDVYATQYSFSLCASPYIPARAKMAGLTRFRLICTGAFLTQIFLHFLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCS8</accession>
    <name>A0A5B0MCS8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCS8"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="36862" checksum="AD27CE0BBA40088F" modified="2019-11-13" version="1">MAQTQFARDMRELANLLGLAPEAQQAPLASTSAHQLPPTILISQPQSSPGPQPFQVAQQYYHYNSTQSAPLPVAGPSNSLSQPAQWQSTLTPMLQHFTASNETFVQPGQQGLNTTWNESAFPTAGPSSLSPANNQLAPGFTPARETDEMNIETTRQGSQSHQKNPALTQEETRVTLKRFSNLKAHMMAHQDPKPIECQVCQLRHAYYRPNELKEHVESLTDPKSGQPLRLRFDKKLHMRKKSDEELSHELRTFGFMCALCESMHTSAAEVEAHLGFHHGRSQQTEVLIHQRTPDEMERAVNKFEHLLGLTTWLVEEYKRLKKQDGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDK6</accession>
    <name>A0A5B0MDK6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDK6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="7884" checksum="1676557FAA21AF6A" modified="2019-11-13" version="1">MQDFGRSGRGREAHESDGTKKQQSSQNQRSSYHVLELMSCTSRDAEKLNSGVPTVSSLQGLLPSCRKTASLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ME94</accession>
    <name>A0A5B0ME94_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ME94"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="81" mass="9198" checksum="66CE7E9B040F65D6" modified="2019-11-13" version="1">MHSLVRCVVNGRISHCFVPSQTAKTKRSDSTIHLAKRQQISPEPPPHPPNLKNTEKNPEKHTKTAPEPKDDQKTRKNPKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEX8</accession>
    <name>A0A5B0MEX8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEX8"/>
    <dbReference type="OrthoDB" id="2499350at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="656" mass="73810" checksum="C069AA32D84C044C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2024-01-24" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFK2</accession>
    <name>A0A5B0MFK2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFK2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="1">
        <location>
            <begin position="36"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="12912" checksum="18DE7B24C3254EA0" modified="2019-11-13" version="1">MSLVDVAFPVSSSRLTTFPSKRSLSSIGSPSNLPKQLKEKVAYQEKKIDASNCKHLKKMATAHIDIAKAAKKQQMALASAMSSQHSTLKHLAYEAIMNKDLTGAYDDVKKYSKLQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJA0</accession>
    <name>A0A5B0MJA0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034368</name>
        <name evidence="1" type="ORF">PGTUg99_035801</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJA0"/>
    <dbReference type="OrthoDB" id="2508716at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="52925" checksum="517D879194C4F44C" modified="2019-11-13" version="1">MPKILDLPWHVFEVIYKHVTNDGDSSSTYDICGLQYDSMMTETGKLRLVCRQWADWIYVHHLYRTMYFSKASEAMRFIINQITRRSKNLPRARCQKLVVLEILTWGPPPPHQGTPTSRRFTPAMFRKPEDTITFEILEALIELFSDTIVELRLTFWNVMLLPTRTIGTIGRIENLRALRLGHELPDPASPQPRLFPLYHDSDDDDIEEEIRQHNARLDEEEMLGNRHRPPVDMNPVKSKIDSDCLKSLLRVTRKLQSLDITDLDPISLPKPIKSSLCGHQIPTITQLEVSLEGKSLSRLIDLSIIFKGTLKVLSLLDRWEGNHSRNLVPVFENLRESLEGLFVSDIKILKPFMKLKFPKLRVFKTGAWEGPISNLLEKPMIAFSPIEVLALKSDVVDSKPKGKFRANPFLNLPTLRRLVFCEVQPGYSAPSAYIKACNARRVECVYLSPKDGEDVSLIMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MK67</accession>
    <name>A0A5B0MK67_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyl-protein thioesterase 1</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_013865</name>
        <name evidence="14" type="ORF">PGTUg99_021814</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MK67"/>
    <dbReference type="OrthoDB" id="2504171at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000716">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="12">
        <location>
            <begin position="173"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1076638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1126803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="432" mass="47630" checksum="4D5BD820DA28F83D" modified="2019-11-13" version="1">MGAGNTFDPFGQLVLKVQPIESSVEALTFKASFLKRSCNGDDLRSLLLSTERVSFKDTFSDSLYHPPRSIGISFRPPRNNIKFYLCIIVVAAALIVMICLTAIGFGGRKHTNTAATPNPNPDSDPSSIPPRERFTSTEYRANWTNPDVDVPQVAYDVIPPVDSINGRGLGWSVLFIHGLGALNASDGYRWRDILLSNYSEPQLTGNSFGNLTGVKFILPKAPIRPITVYAAQENRGARPGWFDIKDWRDLHYLEDEEGLRQSVIGLSTILKNEALAGRIQLNQTIIAGFSQGAVMSLLLTLTLPQPPAACVMMSGYLPLPLRLVDLRSASSDEYRKTSLYWLHGTDDAVLNYNQARAGMKLFSSLFSDRFLRAKFKTFLGLKHGFNMGEQKVATNYIDGIVSRSTSGSFDSSLDASVEPENEGDVIQFNTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKP6</accession>
    <name>A0A5B0MKP6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_002072</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 10">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKP6"/>
    <dbReference type="OMA" id="MNGSFQI"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009396">
        <property type="entry name" value="PRK12756.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF20">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="7">
        <location>
            <begin position="61"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1077291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="382" mass="41059" checksum="C2993CEF86238F58" modified="2019-11-13" version="1">MLKPELTHSRSHSGSSVVNVLDDLRVKNVRPLIPPQILMEDYPVSQTAINTITAGRLGADAIVKGQDDRLLVIVGPCSIHDTKAGLEYAKLLKAYADGAKDHLHILMRTYFEKPRTTVGWKGLINDPCLDGSFQINKGLRIARGFLLECAELGIPVAVEFLDQISPQYIADTVSWGAIGARTTESQCHRELASGLSTPVGFKNGTNGSVGIAIDAIRAAKSGHVFLSVTKQGISAIVETNGNDACHVILRGSNTGPNFSSSHIKECVQELSKAGLKPLVMVDCSHGNSEKKHERQLVVTEDLANQLENPETQDFIMGVMIESNLVEGKQSLEALGPNKLAYGQSITDACLDWESTKKALDRLKAAVGQRRSQKLAAGSSGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPB0</accession>
    <name>A0A5B0MPB0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dolichol-phosphate mannosyltransferase subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_022127</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Stabilizer subunit of the dolichol-phosphate mannose (DPM) synthase complex; tethers catalytic subunit to the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DPM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPB0"/>
    <dbReference type="OrthoDB" id="2014333at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013174">
        <property type="entry name" value="DPM3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08285">
        <property type="entry name" value="DPM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1077854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="128" mass="13912" checksum="9D95A71CCC68607D" modified="2019-11-13" version="1">MGRETSERPGSAGCILPSTGKRNPGQKLIGYLLGFLAVYYSSFFLFFSNNNNTSKGNRASTFECNAVSLGLVPIGLSVVVFGAVCLTKILLDISISNHPKIHEEAYHELLSDIRMAKEDLRSKGISVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTG9</accession>
    <name>A0A5B0MTG9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTG9"/>
    <dbReference type="OrthoDB" id="2421129at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="41"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="186" mass="20448" checksum="FD9DA4B00710C794" modified="2019-11-13" version="1">MKSACSLSTIYTHHSHLSLIRSDRQGLVSSLPSERTLSNDHRTHDDYVKCLAHSPSSAWVASAGLDRRILLWDTVESRPNPLVRFSENTVGTSIYSLAATPTGQLLAAGSPHRLSDLYDPRLMLSGGQASRPHCQCAIHPPLRRCRSSALGLERCDRQDVGRTHPTLSSHLLSHSTSVWALHKPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MV28</accession>
    <name>A0A5B0MV28_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009505</name>
        <name evidence="3" type="ORF">PGTUg99_017410</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MV28"/>
    <dbReference type="OrthoDB" id="10520754at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137287" evidence="1">
        <location>
            <begin position="22"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="97" mass="9980" checksum="3BE776623EBE1C9B" modified="2019-11-13" version="1">MLARAALTLLSCLLIVNMVVATDATPENRLCSGGKIVGILVGGAVIRTDPNGTWKGEPSGKGCQCSSKGFGCLTAPKSGYLPTKTPTICVKLDQPCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWU5</accession>
    <name>A0A5B0MWU5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWU5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="171" mass="18205" checksum="A0A284889DE933CC" modified="2019-11-13" version="1">MRQDELAKMSNTSNSSAATTPAAGTTPVAETAANSPITGRTIQTSTAPYPQPLNRGAANQLEECVNRLKLITIQKKIVPVLVKRPSEATLADGVSTPAVKTPATARATQTMANTAKNPAEKAADAMDIDPQASNSLADTQQLLKLLEVRLLLKKRRFNFSSKSMLPFGRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYB1</accession>
    <name>A0A5B0MYB1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Nucleolar GTP-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NOG2_1</name>
        <name evidence="10" type="ORF">PGT21_024547</name>
        <name evidence="11" type="ORF">PGTUg99_009986</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Nucleus</location>
            <location evidence="2 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class YlqF/YawG GTPase family. NOG2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000013">
        <property type="protein sequence ID" value="KAA1137032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYB1"/>
    <dbReference type="OrthoDB" id="444945at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01858">
        <property type="entry name" value="NGP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000559">
        <property type="entry name" value="Nuclear/nucleolar GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000001">
        <property type="entry name" value="Nucleolar GTP-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024929">
        <property type="entry name" value="GNL2_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012971">
        <property type="entry name" value="NOG2_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF9">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08153">
        <property type="entry name" value="NGP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="9">
        <location>
            <begin position="235"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="491"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="564"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="519"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="564"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="618"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="637"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="652"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="702"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="734"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1080880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1137032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="751" mass="83624" checksum="20D34A078E2777FB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZR1</accession>
    <name>A0A5B0MZR1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ca3427-like PBP 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_004168</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein SsuA/TauA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZR1"/>
    <dbReference type="OrthoDB" id="1363at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054364">
        <property type="entry name" value="Ca3427-like_PBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024">
        <property type="entry name" value="ALIPHATIC SULFONATES-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024:SF47">
        <property type="entry name" value="TAURINE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22384">
        <property type="entry name" value="PBP2_Ca3427_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022882647" description="Ca3427-like PBP 2 domain-containing protein" evidence="4">
        <location>
            <begin position="35"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Ca3427-like PBP 2" evidence="5">
        <location>
            <begin position="121"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1082465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="246" mass="26994" checksum="1C5B4AA688554A82" modified="2019-11-13" version="1">MQHHSNFLPTHSKVLLLLILLLFITLHTPNTAMAATKKLLVGYVPEHFSAPLLQLLENDKELAEWVELVPNPSGTGQMISALKERSIHLAVALTESLIAGIILGKGDYKLIGTYVTSPLNWAVITGANTKYKSIADLKGQKMGISRPGSGSQVMASVMALQQGWANSPDSALEFVVAASPESVDPELLAPEKLKSFLSKLDESIHRFGTELGIRSPEDVKYIQDTFQLGLPHSFSFLRKLEWITHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0R2</accession>
    <name>A0A5B0N0R2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015867</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0R2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022948975" evidence="2">
        <location>
            <begin position="22"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1082835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="271" mass="25086" checksum="E0AD02A0680B5088" modified="2019-11-13" version="1">MNSSMTCMVLLSILLIGSCAALPVGGPPGTNGQSAPSNMAAPTTGGGPGGPGQVASPRAGAPTTGGGNGAPGQAGPPNAGPPTTGAGYGPPGQSAPTNAGPPSTGGGNGPPQQFAPPNAGPPATGGGYGPPGQFAPSNAGPSGTGGGYGPPGQFAPPTYGPPTMGGGYYGPPGQFAPPNAQFPIASGGFGGPAQFTLSSPAPPPIFAGYGSFQPVVAPPIVAAVTPVAVTPPPKSASSIMDIVTKGLSAGGLSLPSAITGAVGSGLYSSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1S8</accession>
    <name>A0A5B0N1S8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_004885</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1S8"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF134">
        <property type="entry name" value="RHO-TYPE GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="47"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="492"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="208"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="347"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="409"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="409"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="447"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="463"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1082486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="68606" checksum="636C2A671CF20771" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N339</accession>
    <name>A0A5B0N339_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N339"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="10744" checksum="C14E0EA1FC21FBEB" modified="2019-11-13" version="1">MSPLPTRLATGVMMLPTGLNQLLGYLPLQLPTKSGIYSDFPISLIRPPPMDCVLPITLDGLRPLLVGLVGDLASNDCKEVTEPIVYLQLWLKFSHKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4K3</accession>
    <name>A0A5B0N4K3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005355</name>
        <name evidence="2" type="ORF">PGTUg99_035706</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4K3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033473738" evidence="1">
        <location>
            <begin position="20"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="61" mass="6707" checksum="4E2909FD7EE63AB4" modified="2019-11-13" version="1">MKFILSTFLLMVFQMHVLATPAPHDELPVGLEDADTRAQASISKRNYTPPGLRCCTGCPIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6M9</accession>
    <name>A0A5B0N6M9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018688</name>
        <name evidence="4" type="ORF">PGTUg99_026529</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6M9"/>
    <dbReference type="OrthoDB" id="1717591at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072318">
        <property type="term" value="P:clathrin coat disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000002">
        <property type="entry name" value="putative tyrosine-protein phosphatase auxilin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172">
        <property type="entry name" value="AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172:SF19">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="40"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="468"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="794"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="831"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="478"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="548"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="794"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1093369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="952" mass="102665" checksum="24D929BE5462AC53" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8E3</accession>
    <name>A0A5B0N8E3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">40S ribosomal protein S24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_011786</name>
        <name evidence="5" type="ORF">PGT21_012646</name>
        <name evidence="8" type="ORF">PGTUg99_017289</name>
        <name evidence="6" type="ORF">PGTUg99_025234</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000506">
        <property type="protein sequence ID" value="KAA1068186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1135989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8E3"/>
    <dbReference type="OMA" id="IRVKKYM"/>
    <dbReference type="OrthoDB" id="5571754at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3370:FF:000001">
        <property type="entry name" value="40S ribosomal protein S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053709">
        <property type="entry name" value="eRP_eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="103"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1064724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1068186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1085577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1135989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15542" checksum="C3AA72DD4F8C33F2" modified="2019-11-13" version="1">MDPSGSVTIRTRQFLTNRLLQRKQMIVDIIHPTRPNLSKDELRDKLSGMYKAPKEQIIVFGFRTQFGGGKSTGFALIYDTKEALKFEPKYRLIRFGMAEKKPAVARKLRKERKNRAKKFRGTQKKKADQAAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NB47</accession>
    <name>A0A5B0NB47_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023828</name>
        <name evidence="4" type="ORF">PGTUg99_013093</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the complex I NDUFA12 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NB47"/>
    <dbReference type="OrthoDB" id="274641at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007763">
        <property type="entry name" value="NDUFA12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT B17.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05071">
        <property type="entry name" value="NDUFA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1113751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="139" mass="16317" checksum="3D79CE3DF8979455" modified="2019-11-13" version="1">MATPSLARTIKNFLALGPREYIRQLWYICDPKPGAFRGSDEHGNKYYEDPSESMFRNRWVDYKVHDFNASQVSPEWHSWLQHIRKDPPHLDPIVVQSRQSWQMPHVENITGTRGAFKTYSTTLPKVQSWEPRVSERTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NCR8</accession>
    <name>A0A5B0NCR8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NCR8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="54" mass="6133" checksum="C1EE7E4F2EE0A031" modified="2019-11-13" version="1">MEWPSRAGQEWVLLRVRLTGGHTQPRLTSAQDGPKRNREADGPYGRPDPPQPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND68</accession>
    <name>A0A5B0ND68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002319</name>
        <name evidence="1" type="ORF">PGTUg99_020671</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000410">
        <property type="protein sequence ID" value="KAA1086474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND68"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="101" mass="11649" checksum="8731C80C794985C3" modified="2019-11-13" version="1">MAMICTLFCKKYFEKRLVSLQVICPVSQIWLNQLSQTKKNLADNCAGQYSAHKPSSHPCEPWIELIHSTESDKKNEDHQNFNPDCNFQLLDQYHSCRSGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI10</accession>
    <name>A0A5B0NI10_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005496</name>
        <name evidence="2" type="ORF">PGTUg99_027197</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000407">
        <property type="protein sequence ID" value="KAA1088224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI10"/>
    <dbReference type="OrthoDB" id="2501084at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="179" mass="20211" checksum="688FD95225068AAD" modified="2019-11-13" version="1">MSYSSPMKMMASRSTKTSTILLPGSQDNNSGFPESRNRRRQSFMANPLESLKLTVSSIGKKSEEPARKVEAEGQEEEETLLQDATNIPARTSFDVALPQRQTQSQVRFQQNARQFPEDWQTSLTPRDSTLRDNRNRRQSFCAAPTSDKSIWNSLGARCKFFRLVVVASPSLQRLASPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIT7</accession>
    <name>A0A5B0NIT7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007139</name>
        <name evidence="1" type="ORF">PGTUg99_028205</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIT7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1088444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="147" mass="16583" checksum="49B1A12D4745594B" modified="2019-11-13" version="1">MSLKDCLQRSLIIYAVICMTLLPSVQLSKLQKRNGRHNCDMQYINVPGSTEDATCTGKDNIKYNCKNWTCTKEFKLHCKHISQPDTGLVGDFTYITPERYMTGPFYITAQAGGVEYGCPYVPENAHRQTCGDCTEDKGDYEPPALLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NT20</accession>
    <name>A0A5B0NT20_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_3</name>
        <name evidence="2" type="ORF">PGT21_000094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NT20"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="282" mass="30646" checksum="B5920E58C88575DA" modified="2019-11-13" version="1">MPPSSGASHVNDTNLSRFALLDLLNEVCAPEPSKSLNYNGEAAPDVTFDPEIQKQVNNLRSRLRAPWLSTITGYKDRIDAHEKQLEEGQQQQDPIVNQSQEHQIPARTITERQPPPSLWAQASNHNSTSGNLPTSDHPIPVGGKKMSLVAVKSASSAYKPPRFNAHPASHENPKASPSTSKQHQPTPETSGPAKDATHTNIPNSANSTSSHHNINMAVSTRFQPPRPIKPKSKQARPSAVASGSVDGDRVTWSHSGPSQPRYTGEEGGFLKEAYHTETDESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTY6</accession>
    <name>A0A5B0NTY6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031355</name>
        <name evidence="1" type="ORF">PGTUg99_031980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTY6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="136" mass="15654" checksum="16994E61E085A86B" modified="2019-11-13" version="1">MKITKISVVVAIFSSLKFCKAPSVGYGIRQNRISEEAWVNTLDVWLQRREEITLRLWGNPKQYSVANQHLTFPVGATVSYGSEQNMIYITNQVNDELEYLMESLDGTQYVSFRLPPYQQDLVPGNAANIWVRAWHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NV15</accession>
    <name>A0A5B0NV15_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NV15"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="230" mass="24843" checksum="1C73886F1216E8FD" modified="2019-11-13" version="1">MWTHLDSPYFPGALINAHPPPSCTLTSPATPKGVCNCICPSLRGGFDCMPLGIILARGVQSNPPLRATPVVRGATRMSSAGRDLMSQRLRVSSSNGLAVLHPWTNQPPQRDRPQTTSPRRSADIPSTPQGIDLDDAFIGRFGGCNIELGVWVGVFVEDFGNRGREVTRRSQRSIAGWSAPGDVPRVVPRDRWRAKLPSRQSKFAPKRPVADPLELLSGRGGPGCTPYTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWL6</accession>
    <name>A0A5B0NWL6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histidinol-phosphate transaminase</fullName>
            <ecNumber evidence="4">2.6.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HIS5_2</name>
        <name evidence="11" type="synonym">HIS5_1</name>
        <name evidence="11" type="ORF">PGT21_017478</name>
        <name evidence="12" type="ORF">PGTUg99_022495</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWL6"/>
    <dbReference type="OrthoDB" id="2015537at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF2">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="39"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1084098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1093333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="431" mass="47017" checksum="C03B7F0EC0218789" modified="2019-11-13" version="1">MVNQNAGLPDHFSLDKVIRPNILSLKPYRCARDDYERGILLDANENSLGSALSPTEEFEQQALPAQQLSPEHGGLNIHSLSLHRYPSPSQIPIKQNLCDYRNSIQPDLPPLEPANVFLGVGSDEILDLLFRIACKPGDSEGIGDQVIVTPPTYGMYSVCAKVNDVGMINVPLNVEGGAFTVDLEHLDSQLSQGSSSRPIKLVILCSPGNPTGTTIPLGTIEKILSNPKFNGILVVDEAYIDFAGNEKSAIKLVRQGWRNLIVTHTLSKGFGLAGIRLGIAYGSADLIQVLNNTKAPYTISSPTSSLGYEATKPDRLKATEAHISKINENRDWLQKELVKITGLGKILGQTEANFILIQVMSFDSPTDVDSQRAKSVYKYMAEDSELGQTPIVVRYRGDELGCQGCLRITVGSRLECQKLVAKLAQTLQLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P785</accession>
    <name>A0A5B0P785_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017133</name>
        <name evidence="3" type="ORF">PGTUg99_035239</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P785"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137683" evidence="1">
        <location>
            <begin position="17"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="135" mass="15064" checksum="556516CF7FDEC108" modified="2019-11-13" version="1">MIWPVIVFCFFSVAIASPAETIFAAGDPGDHQNDMNIFKGLDLEFLESYSGYPRRKIITEEFGHSRSPKRQKIAVGLDGLYNNRPSDSLGSFDQSIGDPLSDHEALNWSDVGCYVEKQNTRHDDTAQAVEPLLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9Y9</accession>
    <name>A0A5B0P9Y9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_026777</name>
        <name evidence="11" type="ORF">PGT21_028337</name>
        <name evidence="12" type="ORF">PGTUg99_005566</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9Y9"/>
    <dbReference type="OrthoDB" id="10264753at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01927">
        <property type="entry name" value="cyclophilin_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000003">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000450">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD-repeat protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="579"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="14"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1096729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1113334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1131682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="725" mass="80907" checksum="213493C3DCD7CF98" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PB69</accession>
    <name>A0A5B0PB69_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012101</name>
        <name evidence="2" type="ORF">PGTUg99_020645</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PB69"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1134043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="59" mass="6214" checksum="6FDA675361F39443" modified="2019-11-13" version="1">MLTSISCSSTTTPIPDMIGPIRKLLTNDAGGLGPMFVIVEATLRPPVPPSNDNLPTYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBP8</accession>
    <name>A0A5B0PBP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026992</name>
        <name evidence="3" type="ORF">PGTUg99_033764</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBP8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366388" evidence="1">
        <location>
            <begin position="28"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="64" mass="6989" checksum="508C1DCCDCEFF373" modified="2019-11-13" version="1">MYKFHWTCSRLALLLVIVVAIGRPVASELKKNCRPGNKCSLQVQPWSRPCTKVEGCGGGRSSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCC1</accession>
    <name>A0A5B0PCC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000064">
        <property type="protein sequence ID" value="KAA1098723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCC1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="377" mass="40637" checksum="FC105C5F86AB3DD1" modified="2019-11-13" version="1">MGESIRRQEGNASGCSIRPTASSDGASLDDTMDSQTRGHTTIQAKDELVQGKEAGPILAEIQNKQPSSLEDKLNEETAAPKDISGSSPFSLSSPIHVDGGLNQENTLATQGCSQPQICLVDDLSAATPTGSDKLSPLEVTFSPTRGSQNQKSTSTTETQDSSPAYQSSVQDNFPDTNDSLLEDPALQEIQEFNTTANLETSQIVDISDSPLNGEENTPAELETDESRHTPNVAYDIPNGENPIPLFNPSSPSPLAKYDVSDSQRCRTVGSPKHQALSHCMQATSVLPSSTPAFCRQDSNEGGMNDSSPSPQQQNKTPTPNRLAAQQLHTSRKEVRKLQYAKKLGIHLTELDPYDLYSDPIWLGDILVELTGVNYSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PDZ6</accession>
    <name>A0A5B0PDZ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PDZ6"/>
    <dbReference type="OrthoDB" id="2511772at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="453" mass="49113" checksum="D1D02D5BDC682B59" modified="2019-11-13" version="1">MLGADSSVNCGRKSLAPQPDLPGSADLASKDTAQVGEHAEEPCLATVNKRLDVDGGRDTLDRTRTGVAVGESGAPAKTRISKGPPDDVGQSTHLASQLAVPAVPLSQPALEKFTGVGLDNSTQDGLDTVGWSAGVSVGLAGMVGNNAAMVDRPDQPEPSMDLEDFTATQDGLLRMLSGLELHARAAEAASGDDVQDQFPDQDGGDPLGPSWSKRVEQALARKRAQEYCALAKAEQEEQERMMVDRYLGIRPVGEESGPGVEFLVGSVPPLDNDQILVLQEVRSWDKINGLELGFMRWEWEGGMDDDLMRPVPTWLIKDGVEVKCFIDDAAHERLARFMGNQPWDFYQLPICYGSAFASRDEWEKAVKNDTLLQPDSPIPDEDIETTATASPDTVVANGSLTLNELYQKIFNPTKGHDVHRFRYESTGPGTPCRRDDGLTGLEDLKLLLGICKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PE12</accession>
    <name>A0A5B0PE12_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_036769</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PE12"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000244">
        <property type="entry name" value="Nonsense-mediated mRNA decay protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="27"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="937"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="949"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1098598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1081" mass="121772" checksum="0BA60C1C568F5252" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIL7</accession>
    <name>A0A5B0PIL7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIL7"/>
    <dbReference type="OrthoDB" id="2511763at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="119" mass="12276" checksum="B05701420EBB68C7" modified="2019-11-13" version="1">MHSGFPKTGGPTSCVPRCMDVSNLTQVLPCSTWGADDLVLRALKAELSAPIGSVCFRGASSARGCVVHPSALAVFAGSVPPDSPSATFPPSASQNQTPTSKTRNQWSSSTSPHPHQASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKN9</accession>
    <name>A0A5B0PKN9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_023490</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKN9"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="323"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="538"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="596"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="636"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="694"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1101523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="742" mass="81183" checksum="B4AC9644B1FC2D4E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0PN04_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1102034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PN04"/>
    <dbReference type="OrthoDB" id="2507153at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="153"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="377"/>
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    </feature>
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            <begin position="416"/>
            <end position="429"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1102034.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
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    <sequence length="515" mass="56336" checksum="2192EB2925462D46" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <end position="22"/>
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            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
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            <dbReference type="EMBL" id="KAA1103057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="529" mass="60679" checksum="4DB33402FFD32889" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0PN47_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_020935</name>
        <name evidence="3" type="ORF">PGTUg99_023716</name>
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    <reference evidence="3 4 5" key="1">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
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        <location>
            <begin position="27"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12754" checksum="1154BADA99982981" modified="2019-11-13" version="1">MHAPGHKKNLVKVLTLLAIYLQPSSSSFHECHCPAKTLTEHWEICNVKLSCRSCGTLGPSWKCTRNVEGQIYKCPQHCKLGHFFPNDPCRDGDHEYDNCSQHLNLRPPVEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PPT2</accession>
    <name>A0A5B0PPT2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PPT2"/>
    <dbReference type="OrthoDB" id="10303178at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="370"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="436" mass="51526" checksum="0F48A73B4B0CA211" modified="2019-11-13" version="1">MDPVQQTNTTQAAIHPDLRTPTRAGTQRRTAMPTRAKAATLRTASHLMPAGPGIEDEGPGSPVVSRRRSYRRPSINPRRRVTAPLSENRPLLDSPMSRRRSLDTQDDRITKRPREQENLSENRLNSLVAAFQFNMEQKIEFLHRHLAWEKESFNHQLEIDNRRIEWEKQIYDRELYPGNPQFEEEKQDFERRLNGEDGQFERNKETYSKDREKYEQDRAERVDGLLAVERCLPAKKQIISMGFRQMMASPSSFPILMAPLVRPNEDRGRLSMQERIDLASMALPENNRQPDVASSDQQSVPSLEPANDGQGEQREGPADQDQCSFVQVFQAYVDQKAQFYKLHTAWENEKLRLRRDLNRRKHEWEMERQARQRKADREEHEQRMKARQEEWEAQRRTLRLELEQLRDQALARQREEQRLGQLHPHHYNPVYQSGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q3B4</accession>
    <name>A0A5B0Q3B4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017385</name>
        <name evidence="3" type="ORF">PGTUg99_028215</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q3B4"/>
    <dbReference type="OrthoDB" id="2503271at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="382"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="526" mass="56229" checksum="0D2BF0DF4948FB50" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6U9</accession>
    <name>A0A5B0Q6U9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1108928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6U9"/>
    <dbReference type="Proteomes" id="UP000324748">
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="202" mass="22206" checksum="31EA4ED0DDC30458" modified="2019-11-13" version="1">MLIFKLQTNPEPTQTRKPAYLNLDSPPYHRRVIVWSPLLFGIFPLSVSGSPNRHRPVSYKVDKAHLAGQEGILLVDEVVQPRRQEGMPERLYNLSGQEGLYSLAGQEGILASKAVQPCRPGGNPPGRQGCTTLPTFPPGGEVVQPRRPGGGNPGRQGCTTLSTTRLYNLVDQVLLVDKVVQPCRQEGIVSRPTRRNLSALRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBY5</accession>
    <name>A0A5B0QBY5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="116" mass="12300" checksum="775849CE98D8EADE" modified="2019-11-13" version="1">MFMGKYLANRPSEHAQDPRIPLGTLGLRSERSLNPSRPSNDPGSLSDAPEPTGSSSDDPDRPPAPQSTPRSASVLPIAHQGLLKAPDAYRQAPRHQATPQSTQQPTGAPPSHPVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCJ6</accession>
    <name>A0A5B0QCJ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="205"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="61"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="205" mass="22281" checksum="B7EDF0A7EA1E505A" modified="2019-11-13" version="1">MRPTSQVQSTPPQVTQTWSTATRPRKRNRIALIGSGWHGQGQKRRSLLPQSNSHPSRSTSPVLDHPTAQLRPKPSNSKLLASPASNTTNPTADEALASVGKRNRIFSSKGQFIPAAPQSTTGLSLPSAQQDGRSNHSESKLLVPLVDDRGLGFCPKGRVIRPAPMIDDPRIKDSSPPVWDRLGNRSPPSYSSARTPNWLFLDPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEE4</accession>
    <name>A0A5B0QEE4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2512028at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="285" mass="30682" checksum="2E55D00F531E0CDB" modified="2019-11-13" version="1">MTEDYSSDFPVPNASPAAPSSPEAVSMDLDVVVEAPPSHSIPAPAPISSPRAVPAFRIRGAANAAPHSSPTPLVPVNQNGGGARARGRARNRGQARGRGLDPVRVRGRGRGFVPAPLRGRGRPSGSNSLPTGPVISNRAVVGRGATPPPAPRGSRLPTPQLSPGGPFERNGSVTSADNSWVEPHVRTRTQFPSPVPFGFYDVDDYHRLAPHQVAALLDRRPDFGRYDQYRFAPQLYTAFYQEVASALVGHFTQHPAANALEREGRVALFRFIGRRYRSAMRRVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QF46</accession>
    <name>A0A5B0QF46_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012467</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QF46"/>
    <dbReference type="OrthoDB" id="2496211at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="308" mass="36028" checksum="2362EEBB637E6037" modified="2019-11-13" version="1">MGYPKSFEESLLLGFPRRAARFRDSQDKLFWNYPRLSEVTNVFRDLAEMLTEDAYWSPEMRLKAIRSWVTDLDKVMALYKDWEELSFNRNAIVSTLDRIKSYEDDIDVPYTYMASDLLPECQEKIKILTKMSLLRLAQDDSGDQYWGLIKSLLPEFQPHQELLDQSSRLAAEKKAVEGAGTAILNKAKKLISLTAYGLADELSDEPRGDWDLFLIYSYGFGLVDSLSENEFIDHKKLQSFFQEEEMAAIVPNYVDMFWRNHDPTRLPLEKGPWSQLNKSFKDLDSNTRRTIEDSFTKTYKKFRLRSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFK7</accession>
    <name>A0A5B0QFK7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_019708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1112000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFK7"/>
    <dbReference type="OrthoDB" id="10453774at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="309" mass="32500" checksum="33F43AAA2635C487" modified="2019-11-13" version="1">MRVWHPSPSSFHIFILQVITPVKQIIFLPFTHPLLPTFKLTSMQSVIFVACLLASAAMGSPILDLGGLQSNPMAYNGGDGIPFNGGGYGGFGNGGLSYSSDRASNAASHQMDSQSSSTPFGSSSSFNQQDASLSSEEHTRLQTRDVGLLGLGQSNYGNGFGNGDGFDNGFGNSGLSYSSDRASNAASHQMDSQSSSTPFGSSSSFNRQDASQSSEEHTRLQTRDVGLLGLGQSNYRNDFGNGDGFDNGFGNGGLSYSSDRASDAASHQMDSQSSSSPFGSSSSFSQQDASQSAREHTDFNSAPNNGFYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFL3</accession>
    <name>A0A5B0QFL3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFL3"/>
    <dbReference type="OrthoDB" id="2496168at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="191" mass="21139" checksum="60471DCB36DDEAF1" modified="2019-11-13" version="1">MTKWDIRNLPQDEEALSSDDEIDLGEVRAPRDRGHTFTETKSSLADRESLLRDLARLATVFDDVREKRPDALTRVDEDDGSEGIECLQEGSTTPDESTPLTADTSAAKRQKITLESPPRNPDTNGISHKHHARKPSMIDCRSIQSPSKEKEKPQEVKRDPGHQAQSGSHPHKIPGPEQPGAFAYPAKSSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIC9</accession>
    <name>A0A5B0QIC9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIC9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="320" mass="35807" checksum="8B848FD39F32242B" modified="2019-11-13" version="1">MTVNRVAERLLFRVLTSLIYISTAAISSHLNASALLQLEDSPHLILKSMFYYQEFSGLSIGFHKAHVLVLSGPSGQRILIIFDNARMKEPQEHEQNVPVTPESNWDLSERQANLNHHSLHSQTHQQILNMGLIIKHYRLFAIFGIFSACEGMQDLLNSFASQRSTPNISNQKSNTQHWIPDLSLTLSTERPSMSNYEHPQGEFDTASALTSLSNTFESPEQLRNAAHELRLAPTSQFLDQRYGISTNAEAQNPSYHQGSCILASGSSSSPAHSFKDPNGIDYPKQWEGHSSRFHKDTFPGGPFAIPPKETTNTNIFSEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QID0</accession>
    <name>A0A5B0QID0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QID0"/>
    <dbReference type="OrthoDB" id="10278074at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="491" mass="53726" checksum="118D5EBDEC0932C2" modified="2019-11-13" version="1">MSQPHDRVTPSQSPAAVTPRNRGRLPLAHVPPNNRFHPHGGSANRSRGSTRGRVSNRGQASNRGHSVSQGGGLVPATPEGGRPQWPNNEGHESANSGWAGATFPPPDPVLDPALNWLGESQPSPTSENFINPGGSNSEGSSDHEDRSSYDPLPDAPALTWGAAPPPPNQETADTAVGGSLVGELQGRLHLSESNLALANRLFRATESEKWGLTVLMFVHLLGKTSDATVSNPLSAPTPEAKNFTFSTHLKCFIRDHLRLILAKGNVDAYTRTMTIANSTPINQTPLLLIKAYVQSQSPSFHKDYLPPGYPRDISASAKVLKLMRGLLKTEKCLLRTLLLHNIKEHNHRPIDGAVPDLDGLVLIIDTFMAARKQARTVADILRSYPASVRTRLAFLRLYVVVHLIHRDPKENISQWELIDQQLEYVKGQSELYRIAYGRVVEAIDKELFGHGVKFEDMDHEDIRVPTDEDVEEEIRVMSASGGSTGESSPFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJG8</accession>
    <name>A0A5B0QJG8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trehalase</fullName>
            <ecNumber evidence="4">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Alpha-trehalose glucohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_026702</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJG8"/>
    <dbReference type="OrthoDB" id="3542292at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018232">
        <property type="entry name" value="Glyco_hydro_37_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403:SF6">
        <property type="entry name" value="CYTOSOLIC NEUTRAL TREHALASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00744">
        <property type="entry name" value="GLHYDRLASE37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00928">
        <property type="entry name" value="TREHALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1113260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="611" mass="70515" checksum="07A560859C0488D7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QK31</accession>
    <name>A0A5B0QK31_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QK31"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="126" mass="14278" checksum="D00ACCE5E9E42E43" modified="2019-11-13" version="1">MDCVPAAPPMISGILEGEDSRPQTTDTPLMEKWDQLKDALANLEVFLKPEANASEDVHASLSSSLVNYPDEMDNWTKILEAWMIKRVNSLKEAHYGLRKEGLKIFGRYADVETNLANKHPFFKLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QKH8</accession>
    <name>A0A5B0QKH8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QKH8"/>
    <dbReference type="OrthoDB" id="10285135at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022970484" description="Hydrophobin" evidence="1">
        <location>
            <begin position="22"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12131" checksum="9F391E46A1460DB3" modified="2019-11-13" version="1">MLQFLRLIALVSLIAPWGVTCEDSEDDAPQERYFGCRRNVDAICTASTEDAHQQNLTWAIRLHHGKRDYKCPEENYPLCCSQGDFKIGSATSFLLVSTDATAFCDPGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMF0</accession>
    <name>A0A5B0QMF0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="14">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">HAS1_1</name>
        <name evidence="19" type="ORF">PGT21_008890</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="14">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="14"/>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMF0"/>
    <dbReference type="OrthoDB" id="10259640at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17942">
        <property type="entry name" value="DEADc_DDX18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000379">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044773">
        <property type="entry name" value="DDX18/Has1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="139"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="329"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="553"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="108"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
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            <begin position="39"/>
            <end position="49"/>
        </location>
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        <location>
            <begin position="64"/>
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    </feature>
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        <location>
            <begin position="582"/>
            <end position="594"/>
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        <location>
            <begin position="612"/>
            <end position="627"/>
        </location>
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        <location>
            <begin position="628"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024310"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1114436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="646" mass="72593" checksum="37C8CC59142C3834" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQS3</accession>
    <name>A0A5B0QQS3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="172"/>
            <end position="198"/>
        </location>
    </feature>
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            <begin position="44"/>
            <end position="55"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="198" mass="21987" checksum="0461740C0158D045" modified="2019-11-13" version="1">MYNQRPKKQDSFFASGPGDCLTNQEQQRLGLIPVDQRHTDTSSRLEPSPHQSIADTSKRRGRRSAMFDQRDRRAAYPYMLSLHSNAKPYQPAPNTLTLSNGNVLQIESHRAQLNYLNTLTVTSPWSLFATIFASGSRKDARKPRTQSDPVEDTNCNPPERILHSSLCSLTSVVSPQPKSSPSSSRVQGVGLPVPRTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QT72</accession>
    <name>A0A5B0QT72_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hydrophobin</fullName>
        </recommendedName>
    </protein>
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    </gene>
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            <taxon>Pucciniomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="142"/>
        </location>
    </feature>
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            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15271" checksum="AB1F0640D206754F" modified="2019-11-13" version="1">MYLFSLLPPSTQFPPILNSNLISSFYKVESAMQSAKFSPVFTTILLLCAVASVASFGCSFGSPCCVEFTNSSQRTLRLAEQVSPEPKIYSCSGQPMSQDTRCCDAQYLPPQNPDGSRTVDISTYNSHCKPAGPNPPPSTSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTJ1</accession>
    <name>A0A5B0QTJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018679</name>
        <name evidence="3" type="ORF">PGTUg99_028412</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000237">
        <property type="protein sequence ID" value="KAA1121773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTJ1"/>
    <dbReference type="OrthoDB" id="10577663at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366539" evidence="1">
        <location>
            <begin position="22"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="177" mass="19884" checksum="DF5C30B2C7DBD1CA" modified="2019-11-13" version="1">MWSIIQPILIGTLFATACVSAVLLEVTHNDKIYWLCSVDHELPADVKPSEVPFRFVGDNVHGKGPKESRFRMMEGTTAKPIWIGNGLPNADHPKWGYGQSEQAKIRFGSTLGALEEDLGYFILVTLNLPGDLAKVEPETWTEILKANRKKLRDAVAREKKNRTQNVLKVQEANHHLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QW08</accession>
    <name>A0A5B0QW08_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009437</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">21-0</strain>
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    </reference>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="140" mass="15703" checksum="7FE29C4901DA768B" modified="2019-11-13" version="1">MWYVKASQYHPCQVSKARDGQRISVAVIRYPLAGIPWLHWIPARGCGCPVSPKIQADIRVSLPGHIQAVGCGLRYAAHSPAAPLGCRHILYEWDADTPTSHFTGLLYRQLTEDKDEVDRPPLRHQLPLQFPPRTARSQAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWS2</accession>
    <name>A0A5B0QWS2_PUCGR</name>
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    </protein>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Involved in the initiation of assembly of the COPII coat required for the formation of transport vesicles from the endoplasmic reticulum (ER) and the selection of cargo molecules. Also involved in autophagy.</text>
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        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        </source>
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        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF12932"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
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                <person name="Pretorius Z.A."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Visser B."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="139"/>
            <end position="186"/>
        </location>
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        </location>
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            <end position="1014"/>
        </location>
    </feature>
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        <location>
            <begin position="1015"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
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            <begin position="1055"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1097"/>
            <end position="1136"/>
        </location>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="208"/>
            <end position="344"/>
        </location>
    </feature>
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        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="512"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="4">
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            <begin position="783"/>
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        </location>
    </feature>
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        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
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        <location>
            <begin position="34"/>
            <end position="46"/>
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        <location>
            <begin position="56"/>
            <end position="74"/>
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    </feature>
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        <location>
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        <location>
            <begin position="290"/>
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        <location>
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            <end position="819"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1119060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1176" mass="128280" checksum="EF8D5885E8055B10" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R274</accession>
    <name>A0A5B0R274_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="311"/>
        </location>
    </feature>
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        <location>
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            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="417" mass="46897" checksum="DBD0F35D30648172" modified="2019-11-13" version="1">MANHLEPSSSSAPQKTHEPSNSSALHHLIRRFTRIPTTLKTLALADTHNHHQPSQSIMSATHQNSPNYKLTLNKNHPLRKVIPSLFLSDPSIDPHPGTSQLSEAESMMSNDSCRKRRMDNLVLAGFLKNNGGYLDDSGSSSASSDLDDTNDQTISSDEDEQDQEHHIHNLNILAKKHQPADTPDGTKTSMAHKTNLYPHQSSTTSNLSKRLLHLHLSNKSDHRKSATVVLNKPINHQTYTTIDPPDDRHFPKSTKDTYDDNYTNINHIEDRPAQGGRFDRLTNNTHFKRPASSKSQSREDNAHPEGGRCQHIKERWYATTGARKPKETGGQEDEEVGLSFNLKYLRSSTFPTKNSILDSQVHTALLHSSPRTLPTRSSSPTITPVDQELRHQHPDFDEFKSPFRNLILGQDDLLRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2S6</accession>
    <name>A0A5B0R2S6_PUCGR</name>
    <protein>
        <recommendedName>
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            <ecNumber evidence="10">1.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Saccharopine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_016844</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-saccharopine + NADP(+) + H2O = (S)-2-amino-6-oxohexanoate + L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 14">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.10" evidence="10"/>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2S6"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004755">
        <property type="term" value="F:saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000008">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000072">
        <property type="entry name" value="Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1870.10:FF:000002">
        <property type="entry name" value="Saccharopine dehydrogenase Lys9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1870.10">
        <property type="entry name" value="Domain 3, Saccharopine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="15">
        <location>
            <begin position="12"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1119174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="759" mass="83793" checksum="4368D1E0AC856892" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3H5</accession>
    <name>A0A5B0R3H5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036471</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3H5"/>
    <dbReference type="OrthoDB" id="2497636at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072:SF4">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="315"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="525"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="587" mass="66662" checksum="A2347F0FA2DE1D6B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4M6</accession>
    <name>A0A5B0R4M6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4M6"/>
    <dbReference type="OrthoDB" id="10308928at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="163" mass="18192" checksum="2601F808F98D0A07" modified="2019-11-13" version="1">MRERERVCEVCDRLRRLERGAACSNCWGPRPDSGAPDQPTTQRRPTPRGRPLKPRWARPGDPDIQILDVQPLSAFHRVRQPAQARGLEAMTSTTPSDYEPESNRSADTNASKAIKNEEPETKLPAVSHELETKPSAETNEGELNASAEENGEPPAKKKRNMTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R5X8</accession>
    <name>A0A5B0R5X8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_050012</name>
        <name evidence="2" type="ORF">PGTUg99_050080</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R5X8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1100154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="155" mass="16673" checksum="5539457D3120CA45" modified="2019-11-13" version="1">MNNVKKKQADELLTRSISPFSYVLHVIASLKGVSSASSTSVIGSGVCLTTLIGLIRKGFLTVEGMGSSSECSSSKQEKSLEGYKQCTPLTHPSHPFWPVSSLVALVCAWAPRPIWPPSLACLVSSSAPRRTQIQMGPSWLKTLICVSVWSLWVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R6S4</accession>
    <name>A0A5B0R6S4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutathione S-transferase, nitrogen catabolite repression regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">URE2_2</name>
        <name evidence="4" type="ORF">PGT21_032755</name>
        <name evidence="5" type="ORF">PGTUg99_026544</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000239">
        <property type="protein sequence ID" value="KAA1121222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R6S4"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01151">
        <property type="entry name" value="Main.2:_Nu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="109"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1118164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1121222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="241" mass="27628" checksum="02B0FB8EA0FB0886" modified="2019-11-13" version="1">MISKSEQKDLHLLTMSTPNGKKVQIALEELKLVYDDISFSHEMIDIRTNQQKSEEFLKVNPNGRIPALIDNKCKALNDPSAPFTVMESAAILLYLAKKVDKNHVFGFEDDLERSEALQWLFFGMAGVGPMQGQLNHFTRYAPEKLPYAMKRYHDETLRLYGVLEIQLSGKYTGVKKKYLAGKGEGKYSWADMAIWPWVDIYTISGITDDEMNQFPCLMEWLKSIRERPGVKEGCSSKYDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R8I8</accession>
    <name>A0A5B0R8I8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcriptional regulator swi6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SWI6_4</name>
        <name evidence="7" type="synonym">SWI6_2</name>
        <name evidence="7" type="ORF">PGT21_036485</name>
        <name evidence="8" type="ORF">PGTUg99_027537</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R8I8"/>
    <dbReference type="OrthoDB" id="6718656at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000001">
        <property type="entry name" value="APSES transcription factor (MbpA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000347">
        <property type="entry name" value="Transcription factor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF3">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="6">
        <location>
            <begin position="43"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="344"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="465"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="142"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="725"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="553"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="725"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="758"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1095191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1121463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="833" mass="92151" checksum="1CD2C96E27ED1680" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RE13</accession>
    <name>A0A5B0RE13_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023000</name>
        <name evidence="2" type="ORF">PGTUg99_023623</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RE13"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1124046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="131" mass="14615" checksum="868FE4D345505297" modified="2019-11-13" version="1">MTKRNLNQATNTSEVVNLNPSMTKSNLNQATNSSELVSNLNPGNMIHWCSLSNLALTLTELSISSDSDDQPIALVKEQLSHEGTLLCQALNHQTTSKNPLKTLVYKESKQKSLWVSLLRRLNYSLKDFTNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RZI9</accession>
    <name>A0A5B0RZI9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PZH1_1</name>
        <name evidence="8" type="synonym">PZH1_2</name>
        <name evidence="8" type="ORF">PGT21_027441</name>
        <name evidence="9" type="ORF">PGTUg99_019101</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RZI9"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF484">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PP-Z1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="7">
        <location>
            <begin position="254"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="54"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1081008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1131160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="378" mass="43048" checksum="39CA54310D3B14E1" modified="2019-11-13" version="1">MGNNPSKKLRTRLREREPSITEEPPADYEPTHSSSPQHQQQQQQQHQHHRKNNSISESTPQPTQSSSSKPLENNQQQQQQQQQYQQQQTNNSNNDQQQQQLNNENNSTILANRPNPVLQTNGFTNTLSTTTTTTTNQAFDIDEIINRLLDVGYSTKITKSVCLKNSEITAISLAAREVLLSQPTLIELSPPVKIVGDVHGQYSDLIRLFEMCGFPPMANYLFLGDYVDRGKQSLETILLLLCYKIKYPENFFLLRGNHECANVTRVYGFYDECKRRCNIKIWKTFIDVFNCLPISAVVASKIFCVHGGLSPSLSTMDDIRRIQRPTDVPDFGLLNDLLWSDPSDTATDWEDNERGVSYCFGKSVIAEFLARPTDGKEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RZR6</accession>
    <name>A0A5B0RZR6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028899</name>
        <name evidence="4" type="ORF">PGTUg99_026587</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RZR6"/>
    <dbReference type="OrthoDB" id="2508036at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366689" evidence="2">
        <location>
            <begin position="21"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="642" mass="73179" checksum="6FBE559E41AEE6C5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RZZ1</accession>
    <name>A0A5B0RZZ1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Transcription elongation factor SPT5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SPT5_1</name>
        <name evidence="13" type="synonym">SPT5_2</name>
        <name evidence="13" type="ORF">PGT21_007676</name>
        <name evidence="14" type="ORF">PGTUg99_005014</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the SPT4-SPT5 complex. Interacts with RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RZZ1"/>
    <dbReference type="OrthoDB" id="28901at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06081">
        <property type="entry name" value="KOW_Spt5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06082">
        <property type="entry name" value="KOW_Spt5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06083">
        <property type="entry name" value="KOW_Spt5_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06084">
        <property type="entry name" value="KOW_Spt5_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06085">
        <property type="entry name" value="KOW_Spt5_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09888">
        <property type="entry name" value="NGN_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000018">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000029">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.940:FF:000005">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041973">
        <property type="entry name" value="KOW_Spt5_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041975">
        <property type="entry name" value="KOW_Spt5_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041976">
        <property type="entry name" value="KOW_Spt5_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041977">
        <property type="entry name" value="KOW_Spt5_4"/>
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    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
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    </evidence>
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    </evidence>
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            <dbReference type="SMART" id="SM00739"/>
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        <source>
            <dbReference type="EMBL" id="KAA1080453.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1131411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1234" mass="133141" checksum="DBDA5C49321BC569" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S2V7</accession>
    <name>A0A5B0S2V7_PUCGR</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1113284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="97" mass="10801" checksum="3EB0E34CE5794268" modified="2019-11-13" version="1">MVERAKITWSKPRPRTPSSSSVTKESNSNTKLRKSTADSNDYSLSLLKTTREGARRVSSSAAGPEETSSLRVPMNHHHQEKPDSVAIRHLSITHEHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SB64</accession>
    <name>A0A5B0SB64_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1093159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1135052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="120" mass="13478" checksum="20F8F12BDD980D44" modified="2019-11-13" version="1">MNSIKSQKGGQAIAGGDKLDLIPFLIDYHVLEEILKDFLTRTVLTNESSSSSTTDNKPVEAGEQLECYEGSFRGRYLHGTQDPRWLFRLSTLILRCPPGCSITLSDKHQQTITRSYSFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SBW8</accession>
    <name>A0A5B0SBW8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002603</name>
        <name evidence="3" type="ORF">PGTUg99_027352</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SBW8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="186" mass="21224" checksum="F66196CB40F850CD" modified="2019-11-13" version="1">MAPSTLFSELSPWYRSRSVSSWTKNIPLKKNDQRSSRADQGLESFELDQRAGLFNPASCGRHSTPPSPPNHTHTHSSNSSRNKIHRQQRLNPDHRRKYISSSSPLAFWRSSRISEPHPHPKDQYSTPTTVPDVTPATGVAAEEDHRWILACGPGQPGRIRSISERSEDPTTINFHFRYRLRNMVDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SDN4</accession>
    <name>A0A5B0SDN4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002646</name>
        <name evidence="4" type="ORF">PGTUg99_027237</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SDN4"/>
    <dbReference type="OrthoDB" id="10534489at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366702" evidence="2">
        <location>
            <begin position="23"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="498"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="565"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="629" mass="65975" checksum="16533BC74B12E3DC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SHP9</accession>
    <name>A0A5B0SHP9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 4E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF-4F 25 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">mRNA cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TIF45_1</name>
        <name evidence="12" type="synonym">TIF45_2</name>
        <name evidence="11" type="ORF">PGT21_022738</name>
        <name evidence="12" type="ORF">PGT21_024217</name>
        <name evidence="13" type="ORF">PGTUg99_007400</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000013">
        <property type="protein sequence ID" value="KAA1137019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SHP9"/>
    <dbReference type="OMA" id="QTEFKMM"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000011">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1074839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1080865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1137019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="310" mass="34517" checksum="7E3A3C16CEB1C65A" modified="2019-11-13" version="1">MAESATSPTFPSSQLPPVAALASSQAVRSALADYTQASSIPSSSVSSPQLDHSSSSEKEDQEEKDDQSSLEDGEIPTEPAGTSSSASNNPITIFSSQTEFNVKHPLYSTWTLWFDNATKNDKAKNWDDLIQQVMQVESVEEFWGLYHNIVPPSLIHVGSNYYLFKEGIKPAWEDPANAKGGSWSIQLSRDRSRDMIDKWWLYTMLAAIGETFETPYTANGKPPATMSFTDEVTGVVISARKMFFRISIWTRSADSKELAQNIGRHFKYGVLSIPETFRFAPSDGKSIQTDCEFRSHTDSQLKKKKPTFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SJT5</accession>
    <name>A0A5B0SJT5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">General negative regulator of transcription subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_003005</name>
        <name evidence="17" type="ORF">PGTUg99_024910</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="17 19">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1933107at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
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        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="2477" mass="276511" checksum="D2AD998180116770" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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                <person name="Schwessinger B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <comment type="caution">
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        <location>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
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    <sequence length="827" mass="91025" checksum="78E81E5D1BC8B053" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LX67</accession>
    <name>A0A5B0LX67_PUCGR</name>
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        <recommendedName>
            <fullName evidence="4">RNA polymerase II-associated protein 3</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
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        </lineage>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the RPAP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LX67"/>
    <dbReference type="OrthoDB" id="629492at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051966">
        <property type="entry name" value="RPAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025986">
        <property type="entry name" value="RPAP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46423">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46423:SF1">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13877">
        <property type="entry name" value="RPAP3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="RNA-polymerase II-associated protein 3-like C-terminal" evidence="6">
        <location>
            <begin position="263"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="131"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="176"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1069055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="333" mass="36154" checksum="765A602F267B26C9" modified="2019-11-13" version="1">MEAGNNKRPTAQVFKDKASCISYTRITSRSVFLDEKLTFVDLNPQGNEAFKKADFQAAVEHYTAAIKADPSDHILPCNRAFARLKLQQLPANYDHHDFEDLKAALALEPNNASICAELEAVKSHLQKDAIIPETSSPPNASTPNSTVSSDSESSSANQPTPGPSSSTALTDKRSSDSSNSLMKEVSTTRFTSSDPKNSTTKAAAGTSFSALKQIRQKKEEKSSGLLTCRNPKTSASSPTDLSKCEAKPTKIPSSSSTNSIPPVRSFADFEMRWGMSSQPELRCSVLEALDQRHVARLFGEHLEPETLEQIIDAMQVLLKSVFQTVLVSNLTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LY42</accession>
    <name>A0A5B0LY42_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_023157</name>
        <name evidence="3" type="ORF">PGTUg99_027636</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LY42"/>
    <dbReference type="OrthoDB" id="2519093at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137132" evidence="2">
        <location>
            <begin position="26"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="613" mass="64807" checksum="1FCCC2FA0C11D95C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M039</accession>
    <name>A0A5B0M039_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024259</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M039"/>
    <dbReference type="OrthoDB" id="2499703at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023082578" evidence="2">
        <location>
            <begin position="26"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="423" mass="48037" checksum="E7E220BE4C8B13B1" modified="2019-11-13" version="1">MSNPRSGRLQSVSFILLIVSVGLSASFLGEVAETTARLSSPLKNSARLTVVHPPEAPHPLPSVSELGGLPTQEHSLPPLKPHSDHPEPLPKPVHLPETHPPDQSLPNDAWQLQTEYYKLGPHLERLTDRLRVVKYMSEPAQSKPAVIVDFFQRYGSLTRPEERLSLVQGAIQDLLKYRQITYELGQHWLKAFEAICQTQKDFESELRMNTLVSFGYRNLMTKLSTPTIAPNPDHVKLERLASNLEADRDQHYQHKIWAADHTGQGRQKTLERKNAIKKVDWSQETLFPYIGLGNSGEVKSFKRMIQESPVVKAYFEKARRRPTWSILLGRYFKRYEKRLIANNEVKFIARLGRTANKANGETDAQTKTASIILLWQLKSRGGIGVHGLFDDFRFTSKAQELATIQINSLIEQKTVGSLWNNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1V6</accession>
    <name>A0A5B0M1V6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000176">
        <property type="protein sequence ID" value="KAA1069940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1V6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="188" mass="20767" checksum="11A6300D90C19EC3" modified="2019-11-13" version="1">MIKGPRAESFQLEVPSLRLTPPQPRAETIQLEVEPLKVPSLCLTPPRPRAENDSARGRARRVTSSRDLELSLFSSRSPPFALPHHDLELKTISARGQARGITSTRALELSLFSSRSPPFASPHHDLELKTIQLEVEPLKTIQLEVLQSSIIPKTDLELNPSQPARGQSTGDLEVSSFQLEVDSLSLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2V8</accession>
    <name>A0A5B0M2V8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016912</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2V8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="12062" checksum="DEE7DB208472AD45" modified="2019-11-13" version="1">MNLDRIERRNKLDFTTFQIISTTCEQPPIHTLEAPDSRTGEALEGRFRWQFLFREFRGLHGIHLAQPPTSSLLSPLRVAHIIYIRSSCPQVIFFDRERFTVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M371</accession>
    <name>A0A5B0M371_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_017923</name>
        <name evidence="2" type="ORF">PGTUg99_021280</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000479">
        <property type="protein sequence ID" value="KAA1071235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M371"/>
    <dbReference type="OrthoDB" id="5876637at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="280" mass="30754" checksum="771DA648F549DAFC" modified="2019-11-13" version="1">MPHKRAKASIRKAESLRKGFDLPPTQHANKKKKRKEKHEASRVKTYEISQDIPKNMFRILNAEKIRAEYKIRKSQDPGSLALPKPSSSSSSSSTPNLQRSNAPSSSSKRRNKSSAQMSKAHDELKILPGEGLGSFNRRVEAALRPKVTAVMKAAKNKLATKKPEATGEPSTVPAPAGPMKETTSIAAESVDEESPKGTTKPVKHFAPRPNRFPITDVAMEPPSLSLTKAMKKNLASSSAAAAASSPLPISAAQKRALELERDKVIKRYRQMKEERYAQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M558</accession>
    <name>A0A5B0M558_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGT21_007333</name>
        <name evidence="18" type="ORF">PGTUg99_005555</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="18 20">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000211">
        <property type="protein sequence ID" value="KAA1123096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M558"/>
    <dbReference type="OrthoDB" id="2507492at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051735">
        <property type="entry name" value="CFEM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928">
        <property type="entry name" value="CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G14090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928:SF2">
        <property type="entry name" value="GPI ANCHORED CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G10580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033473493" description="CFEM domain-containing protein" evidence="15">
        <location>
            <begin position="26"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="16">
        <location>
            <begin position="6"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="122"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1071436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1123096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="181" mass="17964" checksum="9633F5FCE18BD28F" modified="2019-11-13" version="1">MLSNTLRFSTLLVSSLLFSAPATLGQDLSGLPPCAGQCLTTTLATSGKSCTPTDFACLCKNQDFVTASNTCYTSTCPAKDLDNIQTWAVKTCAAAGVKLAANGVTSAANSVSSAANSATNALTGAGGNHTNSTGSASATPNSTPVNSNPPPTRPNSAVALSAEAVKLFAVGSALLSSLLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5B0</accession>
    <name>A0A5B0M5B0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5B0"/>
    <dbReference type="OrthoDB" id="2510764at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="504" mass="56579" checksum="0B5CE11FB173C376" modified="2019-11-13" version="1">MILCRPHWSHRQVDPCLLSTPSNVSTAKDSKAKQAPATEQTIAAQPIKAPKKSSTKIPKKAKKPISPEIVPSEWDTSSDEATKSDKPKPTSSIAPGLKKSSTTSDPFKGNKPIKKLIGSEILKNINIKKSAPKETLTTVTVPIVHTTEFVKRVEKQQPDSPNCSIDSGAAQTTIHQATVSSACPSLPKISNDASVQKKLENYFVPQGRTTRLISSSSSILPSGSFTTKPYKPADSSDLDIITGATAQLWSKPKTSKEPQEDDILQRYRPSFHPLLQTVKLQREYYRKALETKDEDLKIVAWDPIAEYQDYLTGQSGRDYWKKEGITVTPSPSPEVAMRPPEAVQGNQTTESSNQRQPQMNGHYPVEHPPPSVDVHQYQRYQHTPNNPETSQGYRYHPTGIHNQPYRANNRSRNRNRSSTNRRNQEYRAADGFRPRNSNVPRQRTNSQTARENRCIAHQRSIHQEMIRLNRTTQAQYQALEAMREQNAGYGSRRQPQEGGENQSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M711</accession>
    <name>A0A5B0M711_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013257</name>
        <name evidence="2" type="ORF">PGTUg99_021293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M711"/>
    <dbReference type="OrthoDB" id="2498490at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="368" mass="41014" checksum="1D124E2EF431801C" modified="2019-11-13" version="1">MYLFFLAIPLSIALIGLIYYNRQRQQEFEEPPVEGYYVYPPPLPHQSALHPSHYWMAPGAVDPLGYPNHCQAFAPQPAPYKEPSQHQDSLNRNPHAPERLSSFQDPQIRSPQKIAQPGFSNASNTITSEKMLVRDNSSLQLPTPTLDNKKTVLSPIFPGYTREKEASGYINQVPSEQKHDTPPDLSTYQEGLMNRLSDPPSVVEAHIKESFGETVREVKTCDDSSSSNLGRGSAGGADSVDSFPVSMRIVEPVAGTGNAFGRAIVEEMAVPDSPKMDERAPVEQGGLDGRDHQPEEVLPRYTSVDAKVFERYKNRLSLKQDDSYIGRLRDSYHPPETVSPPRKVIRDDNFFIKPSSPAPNPDTNTTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB07</accession>
    <name>A0A5B0MB07_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB07"/>
    <dbReference type="OrthoDB" id="2507265at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="758"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="803"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="827"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="883"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="930"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="953"/>
            <end position="977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="977" mass="110428" checksum="C6E6397538A341B3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MC60</accession>
    <name>A0A5B0MC60_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MC60"/>
    <dbReference type="OMA" id="MVACPEV"/>
    <dbReference type="OrthoDB" id="10453729at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="287" mass="27899" checksum="210D84487A4FCC54" modified="2019-11-13" version="1">MRLFTPRSPFPTSTCISTTYPRFRLNTRFPRLIYLFIFHTTPTASSMQSSLIVSVLIVCSGVIAVPTSRGSDYAVTEPHGDSESYAPTNSYSPPPKSYSPSVEHSSYTQKPSYTNTAVPQYSSGPGQSPRVHAPGPVRFPSGSVGAYPQYGGFGNGGGMLPGFGLGGLGLGANAQVAGGVGGLVEGGTGFGTGIGANALVNVGTGLNAGARVGAGVGGPLVGVGANVNAAVGAGVGGPLVGAGANVHAAAGVAGPTSIAAQATVGAAVSAAPVGVGAAARVGAGVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDQ7</accession>
    <name>A0A5B0MDQ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002643</name>
        <name evidence="1" type="ORF">PGTUg99_009033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000478">
        <property type="protein sequence ID" value="KAA1072509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDQ7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="71" mass="8207" checksum="683206788D6C9C8E" modified="2019-11-13" version="1">MSALDLKEGPHPRITNPHNQLLILASLSVLPSCDRRPDETFNLRRARHDFSRMMPASLFKSWYISSLKRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEJ6</accession>
    <name>A0A5B0MEJ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028117</name>
        <name evidence="3" type="ORF">PGTUg99_028419</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000171">
        <property type="protein sequence ID" value="KAA1126573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEJ6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137238" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="8978" checksum="AE5FDBDD1611037C" modified="2019-11-13" version="1">MQVSLALCFVAFLSLSNQGLSLSDSVEPTRHLSGIPKHQDECKGVTSECGCFKPPKPKSPRGDLGGQVTGGGAVCGGGGGELEGNRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEU5</accession>
    <name>A0A5B0MEU5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEU5"/>
    <dbReference type="OrthoDB" id="2141050at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39218">
        <property type="entry name" value="OXIDOREDUCTASE 14 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12110)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39218:SF1">
        <property type="entry name" value="OXIDOREDUCTASE 14 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12110)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="8048" checksum="D129721C58A10C52" modified="2019-11-13" version="1">MGLATYTATWAAIGFGIRCYQLGVMQRPLFTSELLEERREESKIFQEAQKIRNALRKQQQEQLDSNMSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MF25</accession>
    <name>A0A5B0MF25_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MF25"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023014440" evidence="1">
        <location>
            <begin position="21"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="120" mass="13608" checksum="EDBE360304FAA19D" modified="2019-11-13" version="1">MQLSNILCVLNLLNCYLISAHPTLYPKNLAKREIEGVAFGTQIHHLNKRMEPSTSGGKKLSQEELLALINEKTAYHQERLDQVESNIAKGRAKSIEQQTEILQRTGFLRPKINQDPDKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MK37</accession>
    <name>A0A5B0MK37_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012565</name>
        <name evidence="4" type="ORF">PGT21_013143</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1076608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MK37"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137250" evidence="2">
        <location>
            <begin position="21"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1076608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="127" mass="14182" checksum="267879ECA8A063ED" modified="2019-11-13" version="1">MLFSSILFALYLLNHYGTSAHPNLYPNNTPKIGIEEDVAVDTQLHLLHKRMEGLPNKVKSEGSQAGSSSNVKSAHELLAEAEKNLKKFDKGLGEVSEQVNTNRKKVHFRAAEAHLHSKSLDEQDTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMU2</accession>
    <name>A0A5B0MMU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMU2"/>
    <dbReference type="OrthoDB" id="2510515at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="568" mass="65426" checksum="EA8E7F67AFA85283" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP60</accession>
    <name>A0A5B0MP60_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MP60"/>
    <dbReference type="OrthoDB" id="2506420at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="335" mass="37375" checksum="A6B819FAE7429BA6" modified="2019-11-13" version="1">MAPESTQTQSEIIQDTNGHSTDQSQTQPRQSTRASSVVLTPNMVTPSSDSRVRMTQPESKKRGPGNLSLSASVSNASTSQGQKKRKNKKSRTQIFSEKGKNGTTSLIPNQKENPKGSKAVKKVAKKTTRAENPDGAAFDYDQESDEQSVKIQPQTAKKIKEAMYAKVLDYFEEPVWKKGDREDTALNYKCKWCPGTYRAHPSSLGNLKAHRDGYTQMDKNNKGCVNRDQAKLAGIKLPPSVAEAMVMKEKSKDSKQPGITDFLSVKVAFDNKVLNQIMMIWQIRQALPWSRLEDPYLRAAIQYANHKARLYGRRWVTDSPSSELLLPILLPSGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPH2</accession>
    <name>A0A5B0MPH2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_031885</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPH2"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF13-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768:SF18">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL47-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022927255" description="RING-type domain-containing protein" evidence="13">
        <location>
            <begin position="17"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="582"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="195"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="434"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="512"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="642"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="683"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="721"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="758"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="771"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1078253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="815" mass="89068" checksum="FBD8AA4E83465DFB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRW5</accession>
    <name>A0A5B0MRW5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021974</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRW5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="120" mass="12988" checksum="1321A1AFBB116619" modified="2019-11-13" version="1">MEDLSSLLASLAGSKADSDVKSKDCIKAIGKIKINKASPQNILAQSRGTIETASGSCLVRIRSSNELDITLNHDQLRKMSLVITDYCKQNSGAVVQDKGAFGLNSRTEMRIFFNGKPGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTE8</accession>
    <name>A0A5B0MTE8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RET1_3</name>
        <name evidence="10" type="synonym">RET1_1</name>
        <name evidence="10" type="ORF">PGT21_004525</name>
        <name evidence="9" type="ORF">PGTUg99_021231</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTE8"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="8">
        <location>
            <begin position="24"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1080187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1103915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="114" mass="12731" checksum="8CFD17BE45588E8D" modified="2019-11-13" version="1">MHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIAHGASQLLLERLMLSSDSFELDVCQDCGFMGYNGWCVRCKNGNSVTKITIPYACKLLFQELMAMNIAPRLVLEDAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MU07</accession>
    <name>A0A5B0MU07_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MU07"/>
    <dbReference type="OrthoDB" id="2502284at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="399"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="504"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="560"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="735" mass="80115" checksum="0F7C7082D1626CFE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MU38</accession>
    <name>A0A5B0MU38_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_016257</name>
        <name evidence="5" type="ORF">PGT21_016422</name>
        <name evidence="3" type="ORF">PGT21_017580</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MU38"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137282" evidence="2">
        <location>
            <begin position="22"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1082838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1082845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="80" mass="7244" checksum="BFC276D9A29DE8FC" modified="2019-11-13" version="1">MNFSMTCIVLLSILSIISCAAVPAGAAGGEGAPKGGADGGDKKAKAASILQAVSKGLAAGGLGLSSGVTGAVGSGLKGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUM7</accession>
    <name>A0A5B0MUM7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUM7"/>
    <dbReference type="OrthoDB" id="2509522at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="54289" checksum="02CEB993E1B78B38" modified="2019-11-13" version="1">MNSPANSQSGTVKGSLSQLPTEIKLRIIDHLKLLAEKEGSPGTSFLKLALVDRTFHELCSPTNWKVRLALFSALYTVNLCAHWLPLLDLKSIRKKFLNQFIHDILPHQGKRIRSMTLKLEFSVQYCARRRGPNGIQATDIITDEYGPGRLSEILKVCINVTDLRIFLEHYMDEFGDLSDALVNPIPTSLHPVAQLSNLTYIHLNNQTEDESFKEEYLVNLIRNMVHLVHIRLDRLGASFPTCDFCECPNVIQPILPPLAVHLASLSSLKFIDFSMVDCFDWGWTKMKWKGALEGITLHRSTQLSFRAVHAFCSLFEDSLVSLSLCDVPLSNSGDLPLTSLPKDELEYVFRLPKLKKLAVYTPYSIEFLRLFRESRNITNINLVANYIIGPEDLKSLISHETRIWKDLKSLLVQVKQVGKGRYRPREIADLVACGSQVGVRVECGSLAIEGNRLFEEYQSQFRLGQEWSPDEPSDSESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWI7</accession>
    <name>A0A5B0MWI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017945</name>
        <name evidence="3" type="ORF">PGTUg99_011789</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000378">
        <property type="protein sequence ID" value="KAA1092068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWI7"/>
    <dbReference type="OrthoDB" id="10270442at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="71" mass="8076" checksum="7359680399BFBC36" modified="2019-11-13" version="1">MDSNQASSIGSCSRFQKEDPSRTPYLLNCYVPTRLLVYYHSMVVALEATADKKSVIQNDRIAFSSFWDIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXP9</accession>
    <name>A0A5B0MXP9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033384</name>
        <name evidence="3" type="ORF">PGTUg99_017866</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000111">
        <property type="protein sequence ID" value="KAA1130251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXP9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="114" mass="12702" checksum="B25D193E107F9079" modified="2019-11-13" version="1">MFRQCNFKSITLTSSTLSPTPLFQAKQTHPLPPFTSSPHGGAGMHYPLDPIPALVPEPRRNTRKVRFRKCLHSTTVGRRGIWSPKGQQTFFGISPEDGKKEGPNRELNPGPRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0R3</accession>
    <name>A0A5B0N0R3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015725</name>
        <name evidence="3" type="ORF">PGTUg99_009376</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000408">
        <property type="protein sequence ID" value="KAA1087953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0R3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="98" mass="10517" checksum="BF9CE04AFCB6CEB7" modified="2019-11-13" version="1">MHARGPSDLTFYCDASRSSLVGIPPAGPPREALPRRIMVQDWVARRHASSATGYKMSFTGEASPLATGYTMNGQDPGASDCEPPRTSPIAKDLTVVPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N240</accession>
    <name>A0A5B0N240_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N240"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="64" mass="6868" checksum="1AE18BA7A11511AD" modified="2019-11-13" version="1">MSLVDRSHRPLFPVSTLLRFIISLSSAGGVPYGSVALSPQRFIDQLRLPPQLPLPIASPASPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N319</accession>
    <name>A0A5B0N319_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_001556</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N319"/>
    <dbReference type="OrthoDB" id="2901184at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015140">
        <property type="term" value="F:malate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09317">
        <property type="entry name" value="TDT_Mae1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030185">
        <property type="entry name" value="Mae1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31162:SF0">
        <property type="entry name" value="MALIC ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31162">
        <property type="entry name" value="MALIC ACID TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="504"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="565"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1082379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="633" mass="69847" checksum="E93F218802320BB7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N469</accession>
    <name>A0A5B0N469_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015002</name>
        <name evidence="4" type="ORF">PGTUg99_007002</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N469"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137379" evidence="2">
        <location>
            <begin position="32"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="497" mass="53810" checksum="E3AC05A84E3E0D0E" modified="2019-11-13" version="1">MSTQITRRRSGSHFAQLIIIACILCASSINATPLPIASPSLGLSSQNSYLQPVSHLAKRMEIAGVAGAIATGQAVHNVATAREGAAALAVSNTANDLGKATATVDAMGPAKLQRTTSSPGDLELFKSDIHRNDIEFQDAHSEFPGHKKTLSLDNSLTQKPKTLESQGSAFEYAVKKNEDLVKPPKQLSTGHDTLDPNGASTSHSTQQKPNEVFSPPTETTPNPAPPQPELNKGDVQNPHALGQPEISTAKALEKTPKHVGVPLNPEAATVKPSTLELSRQLWKDTYQPRFQKFISLFVRKQTSKLNGIEVAKLEVPRETPKSLTPGSAPRPPSTEQVPKPSTETPNDLSAAPSNDLSTETPKPVNLADPKAVSPEAEKAAPRTWKQYFSDKHKEFKSQIVAAKQATETASTASRAAPETLESVEQTKSPIRKIWEKFFSQKAPNKEQTKASRFAAFKRYFTGDYWKKLAFWRKPANLRKPAAPTDAQVAPEAAQHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
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    </protein>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        </source>
    </evidence>
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        </source>
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    <sequence length="1703" mass="184850" checksum="860D2A937FEFDDCB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDL0</accession>
    <name>A0A5B0NDL0_PUCGR</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        </location>
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    </feature>
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        <source>
            <dbReference type="EMBL" id="KAA1087425.1"/>
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    </evidence>
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    </evidence>
    <sequence length="87" mass="9728" checksum="DEC6B1AA291113DA" modified="2019-11-13" version="1">MAKELAKRKHWQEEAKKKQQEEEAKKKQQAEEAKKKQQENSSAAASGSTSRGPAESTNDSESDGLEEISKAEFKKKMANSCPKSQKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEC3</accession>
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        </alternativeName>
    </protein>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
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            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF558">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
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    </dbReference>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="695"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="111"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="286"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="420"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="552"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="620"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="288"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="383"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
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        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
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        <location>
            <begin position="560"/>
            <end position="572"/>
        </location>
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            <end position="629"/>
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        <location>
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            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
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            <end position="679"/>
        </location>
    </feature>
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        <location>
            <position position="726"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1087705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="970" mass="105675" checksum="58C135F3BF614BA7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFJ3</accession>
    <name>A0A5B0NFJ3_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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            <begin position="304"/>
            <end position="363"/>
        </location>
    </feature>
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        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="349"/>
            <end position="359"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="439" mass="48823" checksum="2659A7E7214DEA61" modified="2019-11-13" version="1">MSVDSEYPPIYYSVVIQGHRRHSQYRHTVLRLRRTKDTDGKPIGLSRLPPPTIFSCHRVNLPSIHLQYPIYQPTPVYFLPLFPWSIFQLTIMPTYYVNPFGLETPTTTNAYPSLPSTILPDSPELDLPHIDTRSNTPDGQTQLRVDDIPGYKGKLVKVLVAFKLYVVSKNKKKEKTWVPCGSTSEFPVDVTLGVTDFDQFRELVAAACNLHFKSTGGIIRESIEDPVRMPLAWLVSIPRVDEWKKSDKVKMKDASDYQDWLVTAFRAGKKSVGLDIRMTDPAEAKRRAKKEDLLAKREARAAAVKASASKKRKAKNGRGGSGGGGDGGGGGDSGGGGGGGGGPDGAYSEVSSDTDNSGSEIDADDFDDINFHMRSIYRAHPTNVTYDRLIPVYIDPTNPRRYILLLTAAVQTWAKDLVCPFAIVHRWSETEDFWVFVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJZ8</accession>
    <name>A0A5B0NJZ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2511760at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="58" mass="6471" checksum="4B52F30102A45FFD" modified="2019-11-13" version="1">MSHSLYSASNLTQTQRSVHHQSALLQPSTGVWQAGHPREQLFNHDRYSPSSPPSAFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMD3</accession>
    <name>A0A5B0NMD3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_016690</name>
        <name evidence="11" type="ORF">PGTUg99_019774</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMD3"/>
    <dbReference type="OMA" id="TTVWKHE"/>
    <dbReference type="OrthoDB" id="428293at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006862">
        <property type="term" value="P:nucleotide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="16"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="130"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="244"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1070581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1089822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="368" mass="40431" checksum="3003BC33943E37FE" modified="2019-11-13" version="1">MPENSRGSGDESLFGTPAIDQAVSGIGAGCISVLCMHPLDLLKVKLQVSSKPLLANHISLHATTSAPSLVHSKSLSSLHQIIRNDGFFGLYRGLTPNIVGNAASWGFYFMWYSMIKDRMSTDSEGRNIKLSASQHLFASASSGIMTAMITNPLWVVKTRMFTSRAEDSGAYKNLWDGLVRISKEEGLGGLWKGSVLALIGVSNGAIQFMTYEELKRWRQDLIRPDPQRSLNSTETEILPLSNLEYILLSGASKLLAIGITYPYQVVRSRLQNQLFVRQSKGLNSSTQSVRPSNSIPIPSPLTPSTGDVHYRSIAHCILHTYRTEGIKAFYKGLAVNAVRVLPGTCVAFLVYENLSNSFKKLAHQKNLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPN3</accession>
    <name>A0A5B0NPN3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative ER transporter 6TM N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_006557</name>
        <name evidence="4" type="ORF">PGTUg99_028783</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPN3"/>
    <dbReference type="OrthoDB" id="68611at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018823">
        <property type="entry name" value="ArAE_2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37994">
        <property type="entry name" value="ARAE_2_N DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37994:SF3">
        <property type="entry name" value="ER TRANSPORTER 6TM N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10337">
        <property type="entry name" value="ArAE_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="656"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="705"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="740"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="764"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="796"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="Putative ER transporter 6TM N-terminal" evidence="3">
        <location>
            <begin position="99"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="469"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1074182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1090602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1056" mass="117967" checksum="F08EC4E879BAA021" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NRT1</accession>
    <name>A0A5B0NRT1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031148</name>
        <name evidence="2" type="ORF">PGTUg99_031596</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NRT1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="69" mass="7303" checksum="25CB7B9C47C1487C" modified="2019-11-13" version="1">MKSHDKSTPLTLQTSLEMGPAHPDIRSDSDIRSCFQPNQTSASASASSGGYPLALAGIRADTRGYPPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTT6</accession>
    <name>A0A5B0NTT6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_035264</name>
        <name evidence="2" type="ORF">PGTUg99_036571</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTT6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137551" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10574" checksum="564FB4EBFC3AC7E9" modified="2019-11-13" version="1">MHIKSLTAIGCLTGLALGSPMQPKEKSLECRTCCCYRAFSRSETPSNPHITRPPWPRCTDCRQPLPMPPPAQVEVDPVTLLPIKNPNSKCNHHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P061</accession>
    <name>A0A5B0P061_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ceramide very long chain fatty acid hydroxylase</fullName>
            <ecNumber evidence="18">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">SCS7_2</name>
        <name evidence="23" type="ORF">PGT21_032637</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Ceramide hydroxylase involved in the hydroxylation of sphingolipid-associated very long chain fatty acids. Postulated to hydroxylate the very long chain fatty acid of dihydroceramides and phytoceramides at C-2.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18 19">Binds 2 Zn(2+) ions per subunit that likely form a catalytic dimetal center.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the sterol desaturase family. SCS7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="18"/>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P061"/>
    <dbReference type="OMA" id="WTIIEYV"/>
    <dbReference type="OrthoDB" id="2204368at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000007">
        <property type="entry name" value="Sulfite oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005149">
        <property type="entry name" value="IPC-B_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17 18" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="10 18" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 20" id="KW-0349">Heme</keyword>
    <keyword evidence="14 18 20" id="KW-0408">Iron</keyword>
    <keyword evidence="5 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 19" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="22">
        <location>
            <begin position="10"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAA1094915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="358" mass="41858" checksum="060AF196AFFEC977" modified="2019-11-13" version="1">MTADIKKQPPRIFLSEDLNKHNSPTDCWVSYKGKVYNISSFLADHPGGDELLLQYAGRDLGEAMDNPDEHTHSKSAYEMLEEFQIGIIGTPETILNPNLIIDDDFKPTTTDITKDHLRNQFLDLSKPLIPQMWNCQFSRAFYLQQVHQPRHLSRPARLFGPWYLEMFTRTSWYVVPLIWLPIAFALFHRALQQQLDQGNSTPIAWSKNIVCFLFGNLVWTFLEYILHRFLFHIDDVLPDRPFFLLLHFLLHGVHHYLPMDRLRLVMPPILFATLSHPFTRLAYFLFPVPYANAVISGAFTFYVLYDCTHYALHHTQLPKYVKEMKIYHMAHHFKDADLGFGVTSKIWDYAFGTVLPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1K7</accession>
    <name>A0A5B0P1K7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002844</name>
        <name evidence="3" type="ORF">PGTUg99_003332</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000115">
        <property type="protein sequence ID" value="KAA1129969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1K7"/>
    <dbReference type="OrthoDB" id="2514501at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="437" mass="48918" checksum="04285867979E6D1B" modified="2019-11-13" version="1">MKQYQNCKIAPTILIVSMLALMGLVFFNLFAQGKNNHVVPFLSPRFANQTVQKNGENITCQPSPVALNQNLLTIPWTVESNGTRSEEHEEFYGIFQWKIYALTGKGGILSEFKTRTGFLYSAETLNCTIVRARSDHRFQTKEFSFGICGSCVFGDAEFTIEMCASYDSGITTYGSSQIFNRFAREYYELDDFYSRLSIPPNSLRRSAFPPSYNETQVDTYQRTQINRRNVLQFSLSLDRVILIPPAVPGGMSAATDPRSIQSPEGTIIVKDGTMKQSPQGITTHNATVPNMLDAFELSGQSKFRITVTDIVDLLLLGFNSSLAELPDAIQAMFNHTLSINSFLMSTAAREVQGAKIGVNYLCINTKKEWQPFLKVLSITAGSSLGMFTSVFTVITILARRIDSRQKKKDRMSQLEESRVASTLNVREEDIPLTKIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P280</accession>
    <name>A0A5B0P280_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_025219</name>
        <name evidence="7" type="ORF">PGTUg99_023365</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P280"/>
    <dbReference type="OrthoDB" id="282973at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000083">
        <property type="entry name" value="Sphingomyelin phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF34">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="5">
        <location>
            <begin position="239"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="208"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="680"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="683"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1094570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1129014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="760" mass="85789" checksum="994C98A21C25B2D3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2H3</accession>
    <name>A0A5B0P2H3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycoside hydrolase 131 catalytic N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_035200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2H3"/>
    <dbReference type="OrthoDB" id="5283326at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041524">
        <property type="entry name" value="GH131_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612">
        <property type="entry name" value="GH131_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612:SF2">
        <property type="entry name" value="GLYCOSIDE HYDROLASE 131 CATALYTIC N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18271">
        <property type="entry name" value="GH131_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022746436" description="Glycoside hydrolase 131 catalytic N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 131 catalytic N-terminal" evidence="2">
        <location>
            <begin position="49"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1095062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="182" mass="19209" checksum="855BF9B97602635F" modified="2019-11-13" version="1">MQQALLMAIFLFASVSLAVGPLSGMGSVVEERNANTNSGAAGGAPKRIFDFTLTPNMKTADLDNPQSALSKLIAYIVKGAAKASAYVSILHPSNAASQIQLAIRDDSIFIPGGNPANAQHGFRRTDVNPAIDKATTLTGITTWYQTIRLDSKAPLVLTHGYLFASIEVPVSTHSGSPLLDTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5J1</accession>
    <name>A0A5B0P5J1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011328</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5J1"/>
    <dbReference type="OrthoDB" id="341578at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23823">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000203">
        <property type="entry name" value="Chromosome 15, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="13"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="238"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="43320" checksum="60570E6C9F0AAF6F" modified="2019-11-13" version="1">MPPEEDLRQIQETELEALRAIFCEDFEQIRTRSAAKVPDRTMPFDQQYANIHSRFVLKKSNSKIVLGLICVLPRLYPTTRPTLVLEDAIGLSPTHLSELQEQLSAKTNQLIGSEMMYELATFVAEFITEHNMAIASHQPSLIEERVTRATEAERQQRLAIEAEKARQLTLKSFEDLELARQIQKDIDLRHELLKRERDKANTNTGNHYDTKAPADDIARLPFGSLIEIPLPTGLSLKIQVGFEVIDGLLGSVYNCQISNVGPAQLTIVQPHGRYYRSPAPGQRMLSKVASDLDTLRNTSDPHVQSIYGSELVENKLTIVSESLPGNSVRDVLDQCGPFELEKALECLEQILSGLRAVHHAHLVHRGNTFTRFLFVENYSGFQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5T8</accession>
    <name>A0A5B0P5T8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5T8"/>
    <dbReference type="OrthoDB" id="5570013at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="543" mass="58692" checksum="2E9ACAB12594950D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P6Q9</accession>
    <name>A0A5B0P6Q9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P6Q9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="277" mass="29624" checksum="BF10B090622FF2DA" modified="2019-11-13" version="1">MSSAVTPSDSACHPSDACRSRYSSGGSQAGLPPPLPPLVRSEPRFRPSVLPALCSPSAVYTEPRSAVGRDPRRPPMPGFPALDFPPMRRRFPQPACVASAPRAFRISAASIPHAPNKPAKALAKARSRHHIPPTLKILSPGSPPPQLPSHSPIFPPCPPAPTVKSATLRASASPRTIPNSHGRPQPPKRPRTTTRGNSLDDFVVPDSASVSVASPSPSSRPPSPPPQPPQSSPESPAGSDRMTPRPHESNFLSRPEYRVALRSWASRHHVTQPLYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P6W4</accession>
    <name>A0A5B0P6W4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014713</name>
        <name evidence="2" type="ORF">PGTUg99_036503</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P6W4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1132052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="99" mass="11200" checksum="4277AC3166D3A348" modified="2019-11-13" version="1">MVYHKASHIGSHEVERKLRPQPRALSRWCLVPVQGEAFGPPVPISIPINTDKILARLRLPTSSSIVNWIFHASPVFYFSFPSTHRIEHGDWPGSAYRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P850</accession>
    <name>A0A5B0P850_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA decay factor PAT1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_017012</name>
        <name evidence="10" type="ORF">PGTUg99_035293</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P850"/>
    <dbReference type="OrthoDB" id="74835at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039900">
        <property type="entry name" value="Pat1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019167">
        <property type="entry name" value="PAT1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551:SF0">
        <property type="entry name" value="PROTEIN ASSOCIATED WITH TOPO II RELATED-1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551">
        <property type="entry name" value="TOPOISOMERASE II-ASSOCIATED PROTEIN PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09770">
        <property type="entry name" value="PAT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="36"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="860"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="99"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="553"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="553"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1096448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1131986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="949" mass="104688" checksum="11A0A6BC0629A97D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAA4</accession>
    <name>A0A5B0PAA4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026176</name>
        <name evidence="3" type="ORF">PGTUg99_034650</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAA4"/>
    <dbReference type="OrthoDB" id="2506303at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="240" mass="26135" checksum="993B6C12A5C4C820" modified="2019-11-13" version="1">MVAAFLPAHRGRITSRLAKNSFGIHQIPAKQFGSRLSSSFQTQPPPPPSSSSSSSASSSASRVSTPQARALRSIISLYHSSEHFAPLHSNERLVKFIDDSILFKKPPTPYPPAHTPNLLASTLRSLTLNKTILRSGESEQDLNPITSAHRIRAGQLRWLGLKDALCGTSASEIHDDPQHRISRFSSKFSNPSSPSSPSAPAAHPAERLASSTSRINKPKAGLDIVHENWEILQEMSKDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAA7</accession>
    <name>A0A5B0PAA7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7729 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_011465</name>
        <name evidence="3" type="ORF">PGT21_012147</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAA7"/>
    <dbReference type="OrthoDB" id="2536450at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056146">
        <property type="entry name" value="DUF7729"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862:SF1">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24855">
        <property type="entry name" value="DUF7729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137720" description="DUF7729 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7729" evidence="2">
        <location>
            <begin position="55"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1101213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="266" mass="28598" checksum="673B808374146114" modified="2019-11-13" version="1">MAFSHQLISLLVCLAGYQTVIAHPWNVGVGQPQEDQPRVGFRDSLAKKSKLSASCQLAAAGLLASEFGTCANILELVSIFDAKESLISPMNSWITGACSATPCKQEGLAAASQMIRTGCESDLKEGSIAAVAIYSVITHYNATREMFCTQYKENLNYCLPSVLGNVEDQSGENITTDEVMSLIAGKFTVADQSFISVPKETYCNACGQAIVSRSAMMIDAIRQDPVGIEFNYTAGHTVHRISDICGASFENQELPDTVQVALPQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PD06</accession>
    <name>A0A5B0PD06_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PD06"/>
    <dbReference type="OrthoDB" id="2498987at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="31701" checksum="D5A8F96C67F6C93A" modified="2019-11-13" version="1">MKLADQLFHLNRPANPFPEPVPQQPGNLSLGFIYPHPFNPFPKDSGTDCSKLNPIERSGGGFGHHWDLNRSHAQQIESSHFSPATWGDSGRGETSTSFDGPPTSESAPENRSKPSPVPTHFDSQLNGAQLSAFTLMRSNEPLLPKTDGQNSSDSTEKDASPEDDKNSYLNDHIMPWNIISASSSHGDTTAAPSVIQRSEERRPKRWKRKMTDRRREQNRAHQRAFRERHRLSLKQKDVLISQLEERLIRSQEAMVERSEQIRSLSARLDVYFPQRGTSSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PD64</accession>
    <name>A0A5B0PD64_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_032204</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PD64"/>
    <dbReference type="OrthoDB" id="17725at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF23">
        <property type="entry name" value="FERRIC-CHELATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="15">
        <location>
            <begin position="321"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="510"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="537"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1098268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="656" mass="75031" checksum="4B462B0A0A7D8C00" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PD91</accession>
    <name>A0A5B0PD91_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Iron-sulfur clusters transporter ATM1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Iron-sulfur clusters transporter atm1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ATM1_1</name>
        <name evidence="22" type="ORF">PGT21_011922</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating the ATP-dependent export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins. Hydrolyzes ATP. Binds glutathione and may function by transporting a glutathione-conjugated iron-sulfur compound.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140466">
        <property type="term" value="P:iron-sulfur cluster export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000287">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022986729" description="Iron-sulfur clusters transporter ATM1, mitochondrial" evidence="19">
        <location>
            <begin position="17"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="955"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1031"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1064"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1145"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="21">
        <location>
            <begin position="917"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="20">
        <location>
            <begin position="1242"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="169"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1479"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="247"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="354"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="487"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="564"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="600"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="685"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="712"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="749"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="781"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="865"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1479"/>
            <end position="1491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAA1099565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1498" mass="157525" checksum="63304321E73E60C7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGV6</accession>
    <name>A0A5B0PGV6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Midasin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_021976</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the midasin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2501245at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000142">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000712">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002472">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040848">
        <property type="entry name" value="AAA_lid_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048617">
        <property type="entry name" value="MDN1_AAA_lid_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012099">
        <property type="entry name" value="Midasin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041190">
        <property type="entry name" value="Midasin_AAA_lid_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17865">
        <property type="entry name" value="AAA_lid_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17867">
        <property type="entry name" value="AAA_lid_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21108">
        <property type="entry name" value="MDN1_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010340">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="493"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="873"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="1326"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="1607"/>
            <end position="1830"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="2011"/>
            <end position="2162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="4207"/>
            <end position="4472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="4237"/>
            <end position="4257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="4258"/>
            <end position="4270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="4278"/>
            <end position="4305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="4306"/>
            <end position="4324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="4325"/>
            <end position="4337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="4366"/>
            <end position="4385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="4386"/>
            <end position="4417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR010340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1099824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="4472" mass="499923" checksum="CD3A032DA10D65B0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHG3</accession>
    <name>A0A5B0PHG3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHG3"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="529" mass="60121" checksum="005BEBBB7FEC00FA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHV6</accession>
    <name>A0A5B0PHV6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_011064</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHV6"/>
    <dbReference type="OrthoDB" id="3229693at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07489">
        <property type="entry name" value="Peptidases_S8_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000036">
        <property type="entry name" value="Serin endopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010435">
        <property type="entry name" value="C5a/SBT2-like_Fn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034187">
        <property type="entry name" value="Peptidases_S8_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF66">
        <property type="entry name" value="SERIN ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06280">
        <property type="entry name" value="fn3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="136"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="10">
        <location>
            <begin position="353"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="C5a peptidase/Subtilisin-like protease SBT2-like Fn3-like" evidence="11">
        <location>
            <begin position="612"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1101207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="898" mass="97982" checksum="291B0E90E673B084" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIE7</accession>
    <name>A0A5B0PIE7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PhoD-like phosphatase metallophosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_019666</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIE7"/>
    <dbReference type="OrthoDB" id="2100241at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052900">
        <property type="entry name" value="Phospholipid_Metab_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43606:SF2">
        <property type="entry name" value="ALKALINE PHOSPHATASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G03860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43606">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD-like phosphatase metallophosphatase" evidence="2">
        <location>
            <begin position="390"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="721"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="721"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="737"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="770" mass="87316" checksum="4819285F42F11D17" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKV9</accession>
    <name>A0A5B0PKV9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_013358</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKV9"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000003">
        <property type="entry name" value="Obg-like ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF11">
        <property type="entry name" value="OBG-LIKE ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="10">
        <location>
            <begin position="81"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="90"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1101254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="455" mass="50267" checksum="01672D3A1E977E63" modified="2019-11-13" version="1">MPPSLLLLFPSQLLPRLVTVQPIRPSLPLTTNPSHTLKSLFSTSSAQPISATPHLSIKKRMPPKKTVVEKKALLGRPSNNLKIGIVGMPNVGKSSLFNVIAKCDLGKSANFPYATIDPEEARVPVPDERFDWLCSVYKPTNKIPAFLTCIDIAGLTAGASTGAGLGNAFLSHVRAVDGIFQVVRAFDDAEVIHVEGDVDPIRDMEIIHTELKLKDIEWVEKHYETIKKSFRGTGTANLADKAKKEEIDITGKVLAWLKDEGRDVRKGDWNNKEIDVINTLQLLTAKPVTYLINLSETDYIRKKNKWLPKIKAWIDANNPGDLLIPFSVALEERLLQLGVDDEVPARKEELAKIGTTSALGKITTAGYASLHLIRYFTTGPTEVRAWTIRKGTKAPQAAGVIHSDFENKFVCGEIMTYGDLKEHGSEVAVKAAGKYRQQGKPYEMQDGDIAFWKHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLP9</accession>
    <name>A0A5B0PLP9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_024307</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLP9"/>
    <dbReference type="OrthoDB" id="206213at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015938">
        <property type="term" value="P:coenzyme A catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF24">
        <property type="entry name" value="PEROXISOMAL COENZYME A DIPHOSPHATASE NUDT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="61"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1101560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="283" mass="31305" checksum="90A464D88A5AF35B" modified="2019-11-13" version="1">MSRRLSHSLSSILAGSSSVGLDRLSPLTGVDLAHIRLALSSSSANRRLETSPLIEPLGRVKRHAAVLLGLSNIHNRGPGLILTMRSQKMRTHPGEISFPGGHQDPEIDQSLLGTALREAHEEVGLTPQQIEYLGSLEPLLSNSGSTLVWPFVVFIHSLEPQLALRTPTLMIPRLMSRLATAPLRSPGLEDLQGKQQVAEVELVFQISLAALLEPHRQSPSCFRNDPQRPYLEWNVKPEIEIARNNLAGKPLPERPTNSSLTHHKLWGLTGWLIHTFLQTIRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLS4</accession>
    <name>A0A5B0PLS4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030818</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLS4"/>
    <dbReference type="OrthoDB" id="2510939at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="290" mass="32274" checksum="72EE62715300A537" modified="2019-11-13" version="1">MFSLSTLEAGVAQEILQISPANQQAAMVYSAVSIHGKASQTQASLSDVHPIGVTKVQKIRLGNLIKTNLRNFVQKTLMECKLDTYSLEHNTDTGEIVAASLLRMKNKFIADLSAHDKKSLLPTGFIDRNQAVLQAVREQIQETIKYKKGTHQGCCFEVRSGDVPTISKLYNHIQQHLPYGGEFSAPNKISLIAQVQFAYMQLEMIIYALGTSSARAAQWGPIDKQLQYLETKGADYFIVWSNLIIQNDKYFFGSPKKFAQIQLDVSVLPTKDNIQQAMATSCNRRAGNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PM12</accession>
    <name>A0A5B0PM12_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PM12"/>
    <dbReference type="OrthoDB" id="10273831at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023004877" evidence="2">
        <location>
            <begin position="20"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="63" mass="6473" checksum="DF8D8BB98B52B181" modified="2019-11-13" version="1">MQLFLTGLCALLVLSRVGGSPIPINRGMNTPPSSPPDGQNGSNSSHHQNTGGNRAGKAPHDDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNE5</accession>
    <name>A0A5B0PNE5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNE5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1102108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="177" mass="20093" checksum="EA1D9B23E1162108" modified="2019-11-13" version="1">MFKVELINILIDTTYKLLSEPDRTSSIINARQLQETCLLVLAAAHGLSFLHPEQDQGRISSLSDIGYAFLLGSAILVRQDLFWNLSRFLSDKQKYPSMNPWIIIHMNVMNASVWADDRGRITHVAVFLTLTFKLHQSTALPIFHLIYGYMAQLPPPDHRDRPQLSSPVFAAWLFGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTW3</accession>
    <name>A0A5B0PTW3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">GTR1_1</name>
        <name evidence="8" type="synonym">GTR1_3</name>
        <name evidence="5" type="synonym">GTR1_4</name>
        <name evidence="7" type="ORF">PGT21_029932</name>
        <name evidence="5" type="ORF">PGT21_031972</name>
        <name evidence="6" type="ORF">PGTUg99_014389</name>
        <name evidence="8" type="ORF">PGTUg99_027901</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 10">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the GSE complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000414">
        <property type="protein sequence ID" value="KAA1086075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000072">
        <property type="protein sequence ID" value="KAA1133370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTW3"/>
    <dbReference type="OMA" id="LIPNMQD"/>
    <dbReference type="OrthoDB" id="10020193at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11384">
        <property type="entry name" value="RagA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003026">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039397">
        <property type="entry name" value="RagA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF1">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1069707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1086075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1104691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1133370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="335" mass="37329" checksum="1CB559AECDEFAEE5" modified="2019-11-13" version="1">MSQSDAAKKVLLMGKSGSGKSSMRNMIFANMAAADTRKLGATIDMETTPIKFLGHFALRLVDCGGQDSYMHNVLSMPARNRSSVFSQIGCLLYVFDVESPDFSTIDMIWFSRVISAILETLSESENMEEFKLHILIHKMDLVPEEHRQVVFESKAKEVRNKVEDYSKGKISADMIHIFSTSIWDETIYKAWSTIVQMLIPEIGSLHAPLERFAKFTSACEVIVFETATSLVVARWTDNDATEQIQAVDERRFERASAVVKAFKSASARIRSRFKSMEITHPMYTAVLDSLTETTAILVVAVDPKVVPAAIHLNINLSRPSFAKLPGLSPKAPGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYN9</accession>
    <name>A0A5B0PYN9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026801</name>
        <name evidence="2" type="ORF">PGTUg99_014163</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1120904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYN9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1106020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="57" mass="6103" checksum="99B3EB3233E7869E" modified="2019-11-13" version="1">MSNRAFMNHDLICRAYVSTAAGTPITPVAGQNERLLCLVFNQVQALVSVTTAIHGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZX9</accession>
    <name>A0A5B0PZX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peroxisomal membrane signal receptor PTS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PEX5_2</name>
        <name evidence="9" type="synonym">PEX5_1</name>
        <name evidence="8" type="ORF">PGT21_033149</name>
        <name evidence="9" type="ORF">PGTUg99_031051</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxisomal targeting signal receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZX9"/>
    <dbReference type="OrthoDB" id="10006023at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005052">
        <property type="term" value="F:peroxisome matrix targeting signal-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001872">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF9">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="503"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="537"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="37"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="235"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1106319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1109372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="658" mass="73053" checksum="5826C28C8C94961D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q687</accession>
    <name>A0A5B0Q687_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_019731</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q687"/>
    <dbReference type="OrthoDB" id="6159137at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:002147">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:002275">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="102"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="307"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="256"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
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            <end position="651"/>
        </location>
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        <location>
            <begin position="197"/>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="629"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1108633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="788" mass="85034" checksum="B73A9D3568F2156B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6L7</accession>
    <name>A0A5B0Q6L7_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1108828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="866" mass="93234" checksum="F91144768F351E68" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q8Z2</accession>
    <name>A0A5B0Q8Z2_PUCGR</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <location>
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            <end position="16"/>
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            <end position="122"/>
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        <location>
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        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1106407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1109463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="122" mass="13635" checksum="E73869AC4C202465" modified="2019-11-13" version="1">MLHLIRTLFSLNLVLAFTVAAYPSQSALLQNVPHGLGKRFQTVHAEASAPRPWVKTARDFIQEAPQDMTAERAEKRSSQPIPRSFSRIRRRPGAGIATSRQTTGISNPRPWVQKDEAISSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCH2</accession>
    <name>A0A5B0QCH2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCH2"/>
    <dbReference type="OrthoDB" id="10535080at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="50"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="146" mass="15656" checksum="25AE47CD6C1E43EE" modified="2019-11-13" version="1">MANTHVLFQPNRDFFLIGFICDWDMDKGMPIVEVLALSWINPPNTSWVQITGNSNASTGSPSRPGRNVRAAVTQQPTIGGNQEMPVVGGSQGPSNAREHPVPDGDIQAIAGSSRFPQLNEINPSPTAPTTPSPRKRGKMAAPDNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEH4</accession>
    <name>A0A5B0QEH4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RPB5_1</name>
        <name evidence="8" type="synonym">RPB5_2</name>
        <name evidence="9" type="ORF">PGT21_002994</name>
        <name evidence="8" type="ORF">PGT21_003683</name>
        <name evidence="10" type="ORF">PGTUg99_017797</name>
        <name evidence="11" type="ORF">PGTUg99_023528</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000206">
        <property type="protein sequence ID" value="KAA1123771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEH4"/>
    <dbReference type="OMA" id="VRDRGYF"/>
    <dbReference type="OrthoDB" id="248779at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1340.10">
        <property type="entry name" value="RNA polymerase, Rpb5, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005571">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036710">
        <property type="entry name" value="RNA_pol_Rpb5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03871">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53036">
        <property type="entry name" value="Eukaryotic RPB5 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb5 N-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="6">
        <location>
            <begin position="131"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1067391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1111522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1123771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1137832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="204" mass="23474" checksum="4F3E0C32B3195D41" modified="2019-11-13" version="1">MDDDREVSRLHRVNRTIKELVRDRGYEVSDAEINVSLDEFKASDPISNKSTRLNFAAINPETNQGIYVYYSDEKSVGIKTMRKFISILDEKKLESGILIYSQAMTPSANKVIASMAQQFTLEAFQEAELLVNITRHIMVPKHEVMKPEEKKALLARYRLKDTQLPRIQLSDPVARYYGLKRGQVVRITRPSETAGRYVSYRLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFZ7</accession>
    <name>A0A5B0QFZ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFZ7"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="295" mass="33758" checksum="502F1AE4393BAD80" modified="2019-11-13" version="1">MNRQCFARQWRGGDNQLVESDGYWDFPGPAATGEIPLFRDNEPATAYVALTGLTHWAEHRRANAHYMLIGSTRETSPKNNELTFNIDLFSCYVWTQNLPDHFARNFLVNRVLSAPNPPTRNPSQPALDFELSRVVIPEVAIIGMGTSQTDKDRPIPIRIPARTLYTPDSFGTPRTIMERTSEKFHLFVPNLTKDQLRHIDVSVSTRRRSKLSPEEHQLLVPNLTREQRKSIAIQDHFAISDTSAEKYLFVPYLKNSKISYKIYLALPAQPSGAHHRKDNQLKTLKAWDIFPGDTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ23</accession>
    <name>A0A5B0QJ23_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine-zipper-like transcriptional regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LZTR1_18</name>
        <name evidence="3" type="ORF">PGT21_023819</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ23"/>
    <dbReference type="OrthoDB" id="2505591at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="135"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="344" mass="38840" checksum="50E73D47BA412693" modified="2019-11-13" version="1">MPNSENQTITSKRKRVCPGKNVKRWELKKKTALQPFQAASTNPSQEQEDEKGISSTHSATQSNNNDTNNNQKEKIFVYLVPPSHQFDPKPSEAITTQQTLQLYQHFTHGTCVAAPRGEPPFCKVKYVPFDTMSAEELQGWEKLVCHFFDRTNYVAAVKNNGPQMDGSMWADGWRKGSKKESFGRYCSVGKLVKMMLLANYNPQDEAASIKEASDFISIQLQHLAPGVFEKYRETLIENNLPSMSHMEYPLPYDALDFASFLTFTMFDFHNGPHCDTDANHWTLVCWIPIFNPRNCSEDDPILADDDFDMLGAMSNAVVAHINGTAPFLEVAGQEKQVSNAQKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJY1</accession>
    <name>A0A5B0QJY1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcriptional activator HAP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">HAP2_3</name>
        <name evidence="8" type="ORF">PGT21_030727</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJY1"/>
    <dbReference type="OrthoDB" id="1097733at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00616">
        <property type="entry name" value="CCAATSUBUNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="310"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="316"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="333"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1113403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="357" mass="39771" checksum="459A84DECDFDBA2B" modified="2019-11-13" version="1">MSNNNNNHYRGWTTTSVGEENEELEDLLNHLSSAAGDRGLGEGSGLDDETMPAFDDILQPASSSTARDRAGLTALLPFDYPPDGHEHDDDDDDDEDDEDDEDDDEDVDEERKAAVQAAMRGLHTPFTHLSRSERAQTLIEEDGEEEDEQSDEEQVKGGKAEEEEEGWEMARPIEEEGAEGLVKIESEDEEPSALPTISLDHREASPLGLPERPGEEEEEEVDPKEEEEEEEIPATLGGVDAVSSVPEDGPTGSSELVMRDKPGVAGLPIRGPNSTERPVFVNPKQYQRIIKRRLARARLEEMGRLSRERQPYLHESRHKHAVRRPRGPRGRFLTKEELARADHPNIDPTPDPIPTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMI9</accession>
    <name>A0A5B0QMI9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mid2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_009856</name>
        <name evidence="5" type="ORF">PGTUg99_019352</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000204">
        <property type="protein sequence ID" value="KAA1124259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMI9"/>
    <dbReference type="OrthoDB" id="2499299at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138034" description="Mid2 domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1114458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1124259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="37483" checksum="A2EB1D4623AFC332" modified="2019-11-13" version="1">MPIRLLFLLLFLPSTFLSSLKVCHVDALALYEPHNLGWAGHMFQSSSQRAAFLTFLSDNSNTGPELTKEDFSALSILWDALRRTTIEDKQTKSVSQRILSPTSHPLNKIQEASIRLKRRQIATNAAGTLREAPPLNNLLEVSPERFISDSPGTDPPDSGLPAPATSSSKVDSSTGKSATPGPAKAPQKSPGQKASQSTELLDPGLKGLIIIGIVASLMGLYLFFRKRFVSEPEAQISTTSSEKGGLEGPPIAADYAGGINITGPIGDNYTTELSQEMRPGYQNYKQFGGVGNNRAQVKRPSVRSQPTSGKAIALPSSAKVTKQRTRMPSSDQGREGQAKGSNRKPQGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMW2</accession>
    <name>A0A5B0QMW2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010196</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMW2"/>
    <dbReference type="OrthoDB" id="301434at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050471">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433:SF5">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="182"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="271" mass="29710" checksum="5227F8AFE32EFF0B" modified="2019-11-13" version="1">MRASASHRIKRLLHRIGVSDAGPRIALSASEKLMRRIGIASISQCDARYFAGDADVIGTHCHAKIDELASPAGNEDLADRDQSKPAASSRDGFLQSHSASPVSPTKARNHPTNVNNTMASSGPIGLQADHVYRSQLSPWRFKIWSILLVTLDKEMPILESIQRSYRSGPLDRLMIFLACSGWSNQVKHFSRKPDHSVLVLDNQGVGNSDAGPLGIYKTSEMAKDTVDLMEYLGWTEEQSVHLFGVSMGGMISQELGNLSHRIASFHCCKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNH5</accession>
    <name>A0A5B0QNH5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNH5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="119" mass="12964" checksum="6EAC3C6A8B85190A" modified="2019-11-13" version="1">MGELCFNQEWNGGPEQSRFSNILFSLFMVHTRRNTDVPLTIPNFLARITPPRQTLAGQTSVASTFPFPSDSPISSILSSNDKNFRSFSRDMPDPADPANHTSSEASGSNNPTKGDSSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNJ2</accession>
    <name>A0A5B0QNJ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_018754</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNJ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="54"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="104"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="292"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="374"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="385"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1114689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="435" mass="48921" checksum="FB6ADF26F9D3AE01" modified="2019-11-13" version="1">MDPPYGAYNRIRLFLADLNLVLGCLPAPTPYRPVALNSFVSPQNQHWSSLDFHSHLSRKQPTHRSQAWKNTHSRLGREQTRKAGTASKSYPVDFPLIRRSAHVYSRHKHQGPQESSQRSCQSFSTNRHQPSKSCRKHNVQYVAPPYKQDPQSVEDLQGEPYTTECLDQEEPSAANYPNDSAEDHSSLKFQPPCGEMFEDEQINFWSDKSIGDTHELDENKWVDRSDLDINADPDYLEQQPNPAFDLKNYSAEDVDNWASTLSAEEFEHLRIMGPNARTESFRQYAISNLDQPTQTLSSSTHEHLDQPVLPSIGDKDNSDYHHHQPDDIGSNNFDQQQLATPDDESRSTFYNHSDDCNSHLEAFDDNGFDEGFVDGSFDDGRVDDGGFDDGGFDDGGFDDGGFDDGGFDDGSFDDGGFDDGGFDGGLDDGYGSPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNN2</accession>
    <name>A0A5B0QNN2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNN2"/>
    <dbReference type="OrthoDB" id="10286588at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="96" mass="10663" checksum="7AFB6013F305D973" modified="2019-11-13" version="1">MIVDEDISQLEPVIDCNQDPSGANSEADNEKATDKEIDKIIPGTFEVEECEDDEEAETTWMDLVGIAVGLGKNDSHFFANNSQRPSADVRWESVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNT8</accession>
    <name>A0A5B0QNT8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNT8"/>
    <dbReference type="OrthoDB" id="2497971at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="540" mass="61465" checksum="A6EA7F7982E93D37" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQX6</accession>
    <name>A0A5B0QQX6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GmrSD restriction endonucleases N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQX6"/>
    <dbReference type="OrthoDB" id="5419821at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004919">
        <property type="entry name" value="GmrSD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39639">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39639:SF1">
        <property type="entry name" value="DUF262 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03235">
        <property type="entry name" value="GmrSD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GmrSD restriction endonucleases N-terminal" evidence="2">
        <location>
            <begin position="93"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="608"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="674" mass="75418" checksum="52110C9009233371" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QS74</accession>
    <name>A0A5B0QS74_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_54</name>
        <name evidence="2" type="ORF">PGT21_001074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QS74"/>
    <dbReference type="OrthoDB" id="10653131at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="372"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="835" mass="89126" checksum="00C938CF6773D637" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVH1</accession>
    <name>A0A5B0QVH1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002560</name>
    </gene>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">21-0</strain>
        </source>
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    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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</entry>
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            <dbReference type="Proteomes" id="UP000324748"/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1118676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="202" mass="22367" checksum="9947D17985A5DC5B" modified="2019-11-13" version="1">MEENETDLPPLFLCPRQERSTDEFLARCTQPSSLRLSISTCTHTLGSENPEASQSSVRLKTPKRLRLSSYSTQDSMPLIDRIMPADQQTTKINIANLAEGTSLADVMTSFSQFGKIMRCSLKQHYDGQDTSVSATVEFLSYTSAVSAVEELDGATADGLCLHVKLVRVVRPPVHIATKEDSTKQEAQDESPLLHRISKRPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0F7</accession>
    <name>A0A5B0R0F7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013581</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0F7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="134" mass="14893" checksum="B688A9FAA466B44F" modified="2019-11-13" version="1">MEAGNRSPIRIPNRSDESDDAPFASLGLGSLAFLSQVPPAHCYINKYTRPLLCISFLKNYPRLPTLSLSLIPGFQPHRHQASEVTSIPVYRLASSAQPVDQPIQPDLILQQLLDYAPRPWPRVVILVGRSVPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0Q4</accession>
    <name>A0A5B0R0Q4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005311</name>
        <name evidence="3" type="ORF">PGT21_016303</name>
        <name evidence="4" type="ORF">PGTUg99_015246</name>
        <name evidence="5" type="ORF">PGTUg99_016163</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000214">
        <property type="protein sequence ID" value="KAA1122928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000073">
        <property type="protein sequence ID" value="KAA1133113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0Q4"/>
    <dbReference type="OrthoDB" id="2502345at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1122928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1133113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="176" mass="19269" checksum="42605F5CEFF4CEE7" modified="2019-11-13" version="1">MAVPTPTPRNPNQIQSESANPSVTTINIVTTIEKTVTVGLPTAVAQVPSARAYAKVNPVTWVPLVILAILFLFGAMVVGWVILRRKMGNGFRVLLPARKLRKLPRQKRKIVGSPVCATGETLDFENFAINNRPPPTVPAKKKNPRYKDVEMTRLILEGKVPRPPPSPFKPLPPGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1S7</accession>
    <name>A0A5B0R1S7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichol-phosphate mannosyltransferase subunit 1</fullName>
            <ecNumber evidence="13">2.4.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">DPM1_3</name>
        <name evidence="16" type="synonym">DPM1_4</name>
        <name evidence="15" type="ORF">PGT21_008431</name>
        <name evidence="16" type="ORF">PGTUg99_026877</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="16 18">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl phosphate + GDP-alpha-D-mannose = a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + GDP</text>
            <dbReference type="Rhea" id="RHEA:21184"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 13">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="13"/>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000039">
        <property type="protein sequence ID" value="KAA1135456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1S7"/>
    <dbReference type="OrthoDB" id="2603at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006720">
        <property type="term" value="P:isoprenoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000036">
        <property type="entry name" value="Dolichol-phosphate mannosyltransferase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="18"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1118854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1135456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="250" mass="28529" checksum="E6B9BD756F3DC5FA" modified="2019-11-13" version="1">MIRSEGDMESKTNDDKYSVILPTYNERRNLPIMIWLLARTFDQHQIDWEVIVVDDNSPDGTLEVAQQLQRLYGPHRIILRPRAGKLGLGTAYMHGLSTCTGTFTIIMDADFSHHPKYLPTFIALQRAHGLDIVTGSRYRPGGGVHGWDFKRKLVSRGANMLTYILLDPGVSDVTGSFRLYRTSILRELIQKTTSRGYVFQMEIIVRARSMGCRMGEVPITFIDRLYGDSKLGKDEILGFVKGLWKLFWTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2X5</accession>
    <name>A0A5B0R2X5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase I-specific transcription initiation factor rrn3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030729</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRN3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2X5"/>
    <dbReference type="OrthoDB" id="26970at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007991">
        <property type="entry name" value="RNA_pol_I_trans_ini_fac_RRN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790:SF0">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IA RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05327">
        <property type="entry name" value="RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="701"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="794"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="375"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="718"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="797"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="826" mass="93376" checksum="57C0B0A37245B297" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4G1</accession>
    <name>A0A5B0R4G1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fungal-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004019</name>
        <name evidence="3" type="ORF">PGTUg99_009489</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000244">
        <property type="protein sequence ID" value="KAA1120388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4G1"/>
    <dbReference type="OMA" id="ATENTRC"/>
    <dbReference type="OrthoDB" id="5584477at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040976">
        <property type="entry name" value="Pkinase_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248:SF2">
        <property type="entry name" value="FUNK1 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248">
        <property type="entry name" value="FUNK1 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17667">
        <property type="entry name" value="Pkinase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal-type protein kinase" evidence="1">
        <location>
            <begin position="255"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="721" mass="82206" checksum="6F8F88E458B63898" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R6Q4</accession>
    <name>A0A5B0R6Q4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030352</name>
        <name evidence="2" type="ORF">PGTUg99_022419</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R6Q4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1100152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="127" mass="13912" checksum="D482D3200DD01EE3" modified="2019-11-13" version="1">MVTESKHYSLLDGYTEVEGPLSKPGQVFRDGREPQRVSLRVWSRPVAESGSQAVMATMDLSCIRASQAGRSSSLSSHIRLPYISTSRPHSTFSSEKPGVSVLDLKRAMCSADRVIHQPVSTTYQQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RGU2</accession>
    <name>A0A5B0RGU2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ferrochelatase</fullName>
            <ecNumber evidence="12">4.98.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HEM15_1</name>
        <name evidence="13" type="synonym">HEM15_2</name>
        <name evidence="13" type="ORF">PGT21_010066</name>
        <name evidence="14" type="ORF">PGTUg99_008923</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>heme b + 2 H(+) = protoporphyrin IX + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.98.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ferrochelatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.98.1.1" evidence="12"/>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000184">
        <property type="protein sequence ID" value="KAA1125111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RGU2"/>
    <dbReference type="OMA" id="DPYHCEC"/>
    <dbReference type="OrthoDB" id="1323at2759"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1400:FF:000003">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF1">
        <property type="entry name" value="FERROCHELATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 12" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1086730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1125111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="388" mass="42987" checksum="C9EC09B1FCD5DAD8" modified="2019-11-13" version="1">MNCRIISGVRGAYLGQRRAFGSSVQKHGPTGVVFMNMGGPRTVSETGDFLSRLFHDGDLIPLPFQSTLAPFIAKRRTPKIEKQYEDIGGGSPILKWTQEQASGMVKILDELSPETAPHRPYVAFRYAHPLTETCLDEMKRDGVTRAVAFSQYPQYSCSTTGSSLNELYRQLIKDSKDGPSPIKWSVIDRWPTHPGLIEAFAVHVERALESYDPLVRSKVILLFSAHSLPMSVVNRGDTYPSEVAATVHAVMKRLNHSNPYRLVWQSQVGPSAWLGPQTSDALKGYAKKGVNDMLLIPIAFTSDHIETLFELDLEYIEEAKQAGLTGVKRVESLNADPIFVRALADIVAGHLKTSDSSPTSTQMTLRCPGCINNTCLKSKQYFSQQEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RZE2</accession>
    <name>A0A5B0RZE2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002915</name>
        <name evidence="2" type="ORF">PGTUg99_002683</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000115">
        <property type="protein sequence ID" value="KAA1129964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RZE2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1093924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1129964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="81" mass="9010" checksum="7D2DBA99B9F98661" modified="2019-11-13" version="1">MTVISSSTTWTTSSEEPSALRITVDTSSFTRFTTISELCLRNASFDLKLVESGDGQHHDTRLSGCDRHALPTNRRMHAVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S4D6</accession>
    <name>A0A5B0S4D6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029289</name>
        <name evidence="3" type="ORF">PGTUg99_025026</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1133036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S4D6"/>
    <dbReference type="OrthoDB" id="10260712at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="128" mass="14928" checksum="267BA9AD5E627862" modified="2019-11-13" version="1">MMYIRHHTAGRGTADSSAEQNKKHNQTTHRAKRHPWPISNRQDSKRWSRRAHRVLHLQLAKLGFLILPVVSLAKVPQLSSVGDGFDDTYSTQLRPIQKSPDKLYTFVRQRSPVIKFDRRLIGDRFLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SEB2</accession>
    <name>A0A5B0SEB2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017485</name>
        <name evidence="3" type="ORF">PGTUg99_032899</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SEB2"/>
    <dbReference type="OrthoDB" id="2496952at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1136132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="134" mass="15066" checksum="BA17DC4213D35A88" modified="2019-11-13" version="1">MEVYCAELAIKKQAKQGKKEREKAEKEKMKLLKKKSAQSQSHGTRLQSTGTGQSQSSTTSDPNGAFTLEDYELICSYLEEPDNYWRIYGSGEQTEVGPQALSKTASHDSFAIYMNNNCNKQYTLTGNQLQHCLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SFZ2</accession>
    <name>A0A5B0SFZ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024575</name>
        <name evidence="3" type="ORF">PGTUg99_000226</name>
        <name evidence="4" type="ORF">PGTUg99_002176</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000171">
        <property type="protein sequence ID" value="KAA1126407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000025">
        <property type="protein sequence ID" value="KAA1136777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SFZ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1136777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="65" mass="6938" checksum="1C9FD2B9DA7D08B4" modified="2019-11-13" version="1">MSNPNNPNEASPTRFPIQAKPKLALGARTPGQANPASRVPEADRSILLSRARVKVNEPVVGVVHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SJ27</accession>
    <name>A0A5B0SJ27_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005488</name>
        <name evidence="3" type="ORF">PGTUg99_016690</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="745"/>
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        </source>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAA1137780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1241" mass="140748" checksum="A6B46CA3FB55B000" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0SMC2_PUCGR</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1111176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="35"/>
        </location>
    </feature>
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            <end position="239"/>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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            <dbReference type="EMBL" id="KAA1138775.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="400" mass="43799" checksum="EB88BBAC01D2EB41" modified="2019-11-13" version="1">MSKTNSNNLSRSPNTKKDWTASSLSPVSKLLGNRPTVVQSLVSKVEAISFEGTPPSDHHHQHHQQHQHHHQQQPNLPDSSSLSRPSSSLHSPSPQGSPPYSLTSSPNLNDINPHLEEFVRETPTIRQLKTSLRSRLQTDSDRHQKRFSEDSGKSDSTDHHPRQAIHTSPHKLVKPTSLQPISQDISLPPSNIKSALPSSSSNLRPAISSSQDLSPQTTTEISSSHRSSSNHSHGKDPLQTSLANKSCVAKEAQGDYSNLLSVNQQDCDVSFGSNPSATERPIQSRHSTRRSKTSTDLPDLRLPLINFNLSPPTIALWSVEAYNCEQRTTFADLIAHLIILPLHTALAIINKLPVVGGHRDLNPLSSQAEKEGQGILLTASQIAIGLVLASGWKVIRRILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKM9</accession>
    <name>A0A5B0LKM9_PUCGR</name>
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        <recommendedName>
            <fullName evidence="7">TM7S3/TM198-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="ORF">PGT21_003352</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Basidiomycota</taxon>
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    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="2501095at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of exit from mitosis"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140:SF1">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13886">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
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        </location>
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        </location>
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            <end position="266"/>
        </location>
    </feature>
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            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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        </location>
    </feature>
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        </location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
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        </location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="867"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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        <location>
            <begin position="739"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="777"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="869"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="887"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="982"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1073"/>
            <end position="1104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1064450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1171" mass="124866" checksum="E9DCA713C803E6F0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKN3</accession>
    <name>A0A5B0LKN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015693</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKN3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7341" checksum="670BA26D6AC07031" modified="2019-11-13" version="1">MSVKMEKLINMNAALAGSGSRNSVRSFVGGDGETPLDKNKRVHSSSLVRPIAKKHTSLASLGSFQTLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMI6</accession>
    <name>A0A5B0LMI6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMI6"/>
    <dbReference type="OrthoDB" id="2504961at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="123"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="525"/>
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            <begin position="610"/>
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        </location>
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            <end position="360"/>
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    </feature>
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            <end position="138"/>
        </location>
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            <end position="627"/>
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            <begin position="647"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
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            <dbReference type="EMBL" id="KAA1065742.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
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            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="657" mass="72624" checksum="4394F228FB242A55" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMK5</accession>
    <name>A0A5B0LMK5_PUCGR</name>
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        <submittedName>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMK5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="126" mass="13457" checksum="D9210195F327A2B7" modified="2019-11-13" version="1">MSGRLDYPDWSTHRLPPTLRCIVTNLVGSPLDLQHGSMRQLLLSFSSSILGLLSLSFSICCCRSVSCCCRLASRVVDPFVCKVATAGQPTDEPAAGADTIALPLREAFDCLSDEAPEPVAEAIQCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSK6</accession>
    <name>A0A5B0LSK6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024254</name>
        <name evidence="3" type="ORF">PGTUg99_016802</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000009">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2498133at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="263" mass="27839" checksum="40CF6DAD757601B0" modified="2019-11-13" version="1">MSEERTSIFIPRTSLRSPGFIGPVIVATALVVASIFTAPWDDDFSVIKIQDPTFVGFVGIWGACTRYNADNATTANPTATLTGNSTLDAMIHNSGKNRGCSRPGFGWTFSLPVNSTGLAGVPLPNSTIGFNVDPSENTSDQVWVEVMSKSQSHLLVTHVLAGIMATVGLLLLLIPYKPFEKTSPSLAKFIKTGSVSLILVALGAIASLVSFVLTLMTALKVRDTINQIGGGGGTHASLGNIFWFSIASFCLCVPSLWSTRGPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUX7</accession>
    <name>A0A5B0LUX7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007452</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUX7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="82" mass="9182" checksum="8B301C09B2CC7243" modified="2019-11-13" version="1">MLKEPVKNHRPGWNTRSSYQLLDRPALSCCIAKSPSSLFTSSIRLRLGSSVILAPRILALLLQDRRRSTMKGIQVDKKDSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVE7</accession>
    <name>A0A5B0LVE7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVE7"/>
    <dbReference type="OrthoDB" id="2507845at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="864"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="551"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="864"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="917"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="938" mass="99909" checksum="0DD0F25012C15817" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYH2</accession>
    <name>A0A5B0LYH2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYH2"/>
    <dbReference type="OrthoDB" id="2510128at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022706941" evidence="1">
        <location>
            <begin position="25"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="277" mass="31415" checksum="63EAF21382F5B43C" modified="2019-11-13" version="1">MILHLDVLATICVLLAGCPMGCSGRSLWRRMGHDVEMATPLLHSVSTESKHASVPTQYISPAGKSRFGCPFDDPKGSNGIMLSREHPHRGAGTSMPYYGAPVIDEGPIIPLATFRVTAPRVMGVSLDTTWAFTARFLATHRGNYDQSRLAELIRLAKGWIKETVNENHPFVTKYGAAVDSDPVAVSRQLEREFTQLHFDEVIELILPKLSTIKKKYLKEFGLKKGISSQGHLFHPDHHKDRIEQLYKDWKKSCKPLGYYFINLWDKLWRVFKRNKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYI8</accession>
    <name>A0A5B0LYI8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000617</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000179">
        <property type="protein sequence ID" value="KAA1069927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYI8"/>
    <dbReference type="OrthoDB" id="2507344at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022698">
        <property type="entry name" value="OrsD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12013">
        <property type="entry name" value="OrsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="204" mass="22765" checksum="8C0EAAFCDB3255AD" modified="2019-11-13" version="1">MPIPPTGSVTVVPLGDQVMAIPDCPHLAYNNYAKTLICVTCHEDIPTSNVHGHMLSSHKLKVAKAEIIDKFLAYGIKLPEDNIKYPPRKNRVDPCIPVQGLYMYLGYTCTALYHGKPCGLSGCKEKTLSNHLREQHQVSGSGWKDYCETRFVQHLRTDHIKFFSVLYRQPSPEIICSSHLGSGNEIQAEGSILWDKLSQEALCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0B9</accession>
    <name>A0A5B0M0B9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030039</name>
        <name evidence="3" type="ORF">PGTUg99_006960</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000340">
        <property type="protein sequence ID" value="KAA1100459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0B9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="111" mass="12045" checksum="C3C4E24C96D0DDE5" modified="2019-11-13" version="1">MLEEVGSRIRARILSVLIGIKIGTGGPNASPWTGMRHQRPDALDCGRSFSSTSRDPNKHLTPERSETLLGPSQGALQVNQATQRLYDDGAVIQTSTAAQLRTARSKIKICM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0I1</accession>
    <name>A0A5B0M0I1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyltransferase family 25 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031677</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0I1"/>
    <dbReference type="OrthoDB" id="47375at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="49488" checksum="1B309C0F32443442" modified="2019-11-13" version="1">MLTLLATSMLVMYRVREPIKPVSQSHILSEVINSPLPDISLSQSSIDPTLDYRRLLEMTTSPKNFNQHHPTLGFGHIYCISLPNRQDRRETMTKIAAALGVRITFVDAIYKDHPVIGWIGERASEVRKKKLELMSQYSGMDKESIGGMGAGSVWLTVNNGHAANQDFLRNIKLPSLAAARPEFEGKDWVEYLWSVSDHYTLTSSNPDFNITAEMWDPQEKMLPRQINPATVSTYYNHLRVLRTIRESGEASALILEDDVDMEWDLERRWRSIESHLPSDWDTVFLGHCWGHELIQPQFGHPNLHKSTEPLCLHGYAVSALGSRKLLELYNDPWTSFQTPVDTCIPTFIKLGLNSFSIEPTIINQSKVLQSDIQSGKGSSWKGLLVDSVAERILKSEGKPVNHTLTREDSKSNIDPATLFRYKSASIKALKDTQCNKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1U5</accession>
    <name>A0A5B0M1U5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1U5"/>
    <dbReference type="OrthoDB" id="2499248at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="676" mass="74661" checksum="55683858ABA52B15" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3S8</accession>
    <name>A0A5B0M3S8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006536</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3S8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="126" mass="14592" checksum="D7CFFE492C734FE8" modified="2019-11-13" version="1">MSHLIIRGGNFMEEVLKWRCKSWSGMPHTDSFRTGFRPRVQLHNQIKDLGALRPSSLQAPSQVTKCTFFRPASSRPTFDLIRLDQAMMQAREQTIKSSKNCVRCPRFLLISYIPITFMQNDKMVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5D6</accession>
    <name>A0A5B0M5D6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010897</name>
        <name evidence="2" type="ORF">PGTUg99_028595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5D6"/>
    <dbReference type="OrthoDB" id="2509931at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="104" mass="12422" checksum="420F6BE1D7FC21A9" modified="2019-11-13" version="1">MHNTSQMIAPETKRVTPSKTEDFVQLGLTAALTEFEDYLAKNRDERSNIEQQLMKKAVALEKEIDRLRNALWLVNARIEATMYLSTSHRRRRLIRHRRHARRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5Q7</accession>
    <name>A0A5B0M5Q7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5Q7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="68" mass="7784" checksum="338CFFC1A61632F6" modified="2019-11-13" version="1">MPKITKENEDGNEGDNEDSDSNSEAGSDHTNEDDPELDDDDQDDTDDSQEPNRRPKRIFLKGQQEQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6V5</accession>
    <name>A0A5B0M6V5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018821</name>
        <name evidence="3" type="ORF">PGTUg99_016295</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000174">
        <property type="protein sequence ID" value="KAA1125890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6V5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="109" mass="12011" checksum="D78450EEEF2F109B" modified="2019-11-13" version="1">MNTPESRVTRHEEENSAGNEVVDPALDVLNHQTTQSWTACEDLATNGQEDHEFLDAEDNTFSDNELDLCASDLDTSSDLSDSSQGRIYCVLVLLFFSNPTGAPGWSWGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB77</accession>
    <name>A0A5B0MB77_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012943</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB77"/>
    <dbReference type="OrthoDB" id="2504541at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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        <location>
            <begin position="87"/>
            <end position="111"/>
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    </feature>
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        <location>
            <begin position="261"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
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        <location>
            <begin position="383"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="550"/>
        </location>
    </feature>
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            <begin position="630"/>
            <end position="697"/>
        </location>
    </feature>
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            <begin position="175"/>
            <end position="202"/>
        </location>
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        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="283"/>
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        <source>
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        </source>
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        <source>
            <dbReference type="EMBL" id="KAA1073463.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="697" mass="78774" checksum="9109F66EEEF0FD5A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB94</accession>
    <name>A0A5B0MB94_PUCGR</name>
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            <fullName evidence="5">IMD domain-containing protein</fullName>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:phospholipid binding"/>
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        <property type="project" value="InterPro"/>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="56692" checksum="F43DCA3D5F7F0426" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MC87</accession>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
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    <reference evidence="6 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ME92</accession>
    <name>A0A5B0ME92_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ME92"/>
    <dbReference type="OrthoDB" id="10294923at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="257" mass="27763" checksum="90D31EA4D7C72245" modified="2019-11-13" version="1">MTDDLWFLPASADYTIVNFLVMYKPLNQFYFFVVVAISLLAYCSPHPKPHGSTGALTSELVNILPVFGDLASDLQLGSSDPGDHHDTTPDALVNAQKAVTLKVAQPERAWVKTNNSPRRRQSTMFLDFLDTFNESSSPSDADAPRPIDSSVAATSRPTPEAVVVPLPVTPVTDQPIPSSVDDSSKTVTEAESSPPVHVLPSFLPSRMPGLSTIPTKKKAAKTKRSLPDNLLSGAFPFSPLRLGSLSTLSLMVLDNQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEI8</accession>
    <name>A0A5B0MEI8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003756</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEI8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="112" mass="12442" checksum="ACAAD434E075DD8F" modified="2019-11-13" version="1">MPTRVKRKRRGQLAGWKGRRKTLQLALGMIALKGSSGRIGYVRLRCVEVTALYKVLIHSALAFFDPSSPLSMSACRLKCMVLQQQPLDVPPPSAELWYLVQGQSQPFASSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEK6</accession>
    <name>A0A5B0MEK6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008908</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEK6"/>
    <dbReference type="OrthoDB" id="2496461at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="176" mass="19471" checksum="E92F8210216010C4" modified="2019-11-13" version="1">MELKMISPADLAKDVRTAVKVKQHVITQGAAQLVSGPSNSVSAEEFNVINVYMNQIYETHPPNVKYKDKIPVDLHPTDLSRYIPLTPANVQEWAAALQNQASACGNDVTDPIRYGIGQFIGSDRFFVTRYRKKGYWIAHPIRYPEYRIAAGSIAGLGIGIASRYRSDSDPVTVGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MF67</accession>
    <name>A0A5B0MF67_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_019638</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MF67"/>
    <dbReference type="OrthoDB" id="185455at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022791220" description="RING-type domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="55"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1074768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="136" mass="15210" checksum="15199CABC47480D9" modified="2019-11-13" version="1">MLQICILMVAMIIEAIIATPIIHPSDQTGSNILLELSNHHIKRNLNDGEVSSMICSVCIEPVSKDLACHREGCKHPFHADCVRPLFNHQKTAQCPNCQALGPNPSETQTAAQDQRVQYQIRHHTPSETTYYNFFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFT1</accession>
    <name>A0A5B0MFT1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026968</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFT1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="122" mass="13644" checksum="199DD1C9EAA1CF28" modified="2019-11-13" version="1">MPENTTTNPENQNKVDLPQKLSDLQIEEILSCTKVEATNPSDQAHLNDIELGDLDEKIAEKLDQIKARSAALMSNPNGESSRWLLRQEPGAPKRRWLVARNIENEVEISVDLMGLEGKPEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFX0</accession>
    <name>A0A5B0MFX0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mitochondrial GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MTG1_1</name>
        <name evidence="5" type="ORF">PGT21_027356</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFX0"/>
    <dbReference type="OrthoDB" id="269151at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01856">
        <property type="entry name" value="YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:005958">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782:SF4">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="4">
        <location>
            <begin position="25"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="421"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="452"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1075023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="462" mass="51122" checksum="490B20B7757929FE" modified="2019-11-13" version="1">MTIQRSFTFPSVLPTWHLGHMVKAIRDMKQRMNDVDVVIETRDARLPLTSINPVFEEILKGPSVKNRAPSASSSSPIRLIVYNKKDLADPRLEKPLKDAFKKHGQTVFFTNARIDEDVRQILRLSKELACPRILSRYGKAAPSSETISTNLVQSQSRKKSSLSSLDPNRGIRIMFCGMPNVGKSSLLNALRRVGCHKGKTASEAPMPGHTRSIGGMVRVSEGSKQTAGWPVYAFDTPGIMVPYLGQGEEAGDRAFKMAITAGIKETLFDPYDLAAYLFHLLLLRHSSPFDAISRISEVLKLPPTFSSSIKDHLDASQPLPTEQLLSGIADRIKAVEVGGVPNLEAAAQWMLKCFRDGRFGGWTLDDVGQESIGAKVCNSSETSIIRLIESNSQTNQPIDDRALTENSTHIEGCISSYFASDRTSPKSKTAVKMEARRAKKDVQRAQQELKPIKHRPPQSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFZ1</accession>
    <name>A0A5B0MFZ1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phospholipase D</fullName>
            <ecNumber evidence="1">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_026086</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFZ1"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00138">
        <property type="entry name" value="PLDc_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000032">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF186">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1074976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="394" mass="44467" checksum="9823EE376CF86ABE" modified="2019-11-13" version="1">MAIMDWTYKSICRGPGSIFSHIQTQGVDPRKYISFYNLRSFDRIAYAPETLKEIEKSAGISYYEAEAALARVYLGESASSQELEKNKEVKFKLAQKGESMEAGTRDMMARDGDTIKVELPKSVDEARQRLKSWSEAASRHNREVPASIATQNNSNGLENLPWLGCEQSERDSFVTEELYIHTKCMIIDDQKVIMGSANINDRSMVGDRDSEIALVVEDQDMIESTMAGQSWKAGRFAATLRRRLYKEHLGLLPPQSNSLQPNEPTRSMLPVNVPQEDDMGRGEDQIVADPMGRELEEMWNGRASVNTQAFNKVFRCVPAAGILNWKDYEEYVPSGPNAPKVCHVAPTAGDVHEVKRELSRIQGHLVEMPLDFLEQDKMYREGKAVNLVTMDIYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHX4</accession>
    <name>A0A5B0MHX4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_32</name>
        <name evidence="2" type="ORF">PGT21_000606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000147">
        <property type="protein sequence ID" value="KAA1075759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHX4"/>
    <dbReference type="OrthoDB" id="2507470at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="526"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="536" mass="60422" checksum="B3222199459A8376" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIL2</accession>
    <name>A0A5B0MIL2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIL2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="119" mass="13135" checksum="49AEDD22A1D59361" modified="2019-11-13" version="1">MALGLDLFELSTFSCKKWYMIRLIENGPSGLHPGTRSSAARLHQASGQAILFTAYITPTGKPSGPANRKSISSTANSSIRVRLKYFPSSLSDQTRQECPTQTVHRPHPIKPDKLKVKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJ70</accession>
    <name>A0A5B0MJ70_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033764</name>
        <name evidence="2" type="ORF">PGTUg99_035270</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJ70"/>
    <dbReference type="OrthoDB" id="2497669at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366156" evidence="1">
        <location>
            <begin position="19"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="128" mass="14133" checksum="27DAED9584827967" modified="2019-11-13" version="1">MLVKICWIVCFLLASVAALHPKVVSGSDQNDCLQSAAKTTEPGARKQHFNKGISNGSGFLFASQKYNFKNRGSKSAGMRNRIMKDLAAFKMKFSDQDEVSVRWTIDVNTPDVPTASATRKIKVQLPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJB4</accession>
    <name>A0A5B0MJB4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PRMT1</name>
        <name evidence="9" type="synonym">PRMT1_2</name>
        <name evidence="9" type="ORF">PGT21_037121</name>
        <name evidence="8" type="ORF">PGTUg99_001673</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="EMBL" id="VDEP01000466">
        <property type="protein sequence ID" value="KAA1077035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJB4"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="7">
        <location>
            <begin position="64"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1077035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1115587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="242" mass="27452" checksum="3F6F1BA8A7356E60" modified="2019-11-13" version="1">MAQANGGQSSSELKTAESLTSKDYYSDSYAHFGIHEEMLKDEVRTLSYRNSMIWNQHLFKDKVVLDVGCGTGILCMFAAKAGAKQVIGIDMSGIIDQAKKIVKANGFEDTIVLIKGKLEDVELPVDKVDIIISEWMGYFLLYESMLDTVLLARDRYLKPGGLMFPDSATLFMAGLEDQDYKEEKINCMSLAFYSFCSRMYDRHLINTLSFSWVDKFGMMSMDLTTRVSRRLLYGNPWLTPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MK53</accession>
    <name>A0A5B0MK53_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007921</name>
        <name evidence="2" type="ORF">PGTUg99_007913</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MK53"/>
    <dbReference type="OrthoDB" id="2246127at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1135500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1198" mass="135399" checksum="A89F4E917EB5622B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKF2</accession>
    <name>A0A5B0MKF2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycoside hydrolase family 5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_013686</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKF2"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050295">
        <property type="term" value="F:steryl-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904462">
        <property type="term" value="P:ergosteryl 3-beta-D-glucoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000091">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000122">
        <property type="entry name" value="Glycoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000106">
        <property type="entry name" value="Glycosyl hydrolase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041036">
        <property type="entry name" value="GH5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF6">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 5 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18564">
        <property type="entry name" value="Glyco_hydro_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="5">
        <location>
            <begin position="138"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5 C-terminal" evidence="6">
        <location>
            <begin position="738"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="65"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1077617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="998" mass="110905" checksum="57E92F214F9A63B7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML92</accession>
    <name>A0A5B0ML92_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_022297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML92"/>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="85"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="16055" checksum="D0B5FD013C8D1CFA" modified="2019-11-13" version="1">MNIMPPKRRLTVELEGSRLNHHRNQSAAEHIFIFRRRRRSYHRRKHHSIRRCSDLVACRGSCFPLRWTLGTVPVWSLSDNNPSAGVRLGLRPLPPQQASMTPRAAEDHEDDPQGREDDDDSHFVKLTRRESGSSGLELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MM32</accession>
    <name>A0A5B0MM32_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MM32"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022834362" evidence="1">
        <location>
            <begin position="19"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="62" mass="6736" checksum="117F45ED3DA711D1" modified="2019-11-13" version="1">MNLIKLSIALIINTACSALLAAAQTIISFNCKNPAYKVALCVIACRSRGERNVHYNCKSFSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMI7</accession>
    <name>A0A5B0MMI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMI7"/>
    <dbReference type="OrthoDB" id="2510764at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="301" mass="33137" checksum="078EC30F3506F3DF" modified="2019-11-13" version="1">MLTYCDPKTSHQRQVTLETGADNWGSGRLLISLATSLIRTDYYTSDHSSHLAKAHQSGPAPSTTAPQINHSMSTPANKSVYTTTAAEQASATKPIGAAHPLKASKKPVARVPKKAKKPISPEIVPSEWDTSSEEDTKSDKPKPKSSAAPELKKSSTTSDPFKSKKPIDKLIDKDILKNITIKKSTPAEKTSSLASAPSVPTDQTHASFTTEPYEPADSSDLDVIAGATAQLWSKPKTSKAPLAEDILQRYHPLFHPLLQAVKLQQEYYRRALETNDEDPPLGKLLPPLHLRYPNYFWFWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRR8</accession>
    <name>A0A5B0MRR8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000136">
        <property type="protein sequence ID" value="KAA1078716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRR8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="87" mass="9216" checksum="651908B043BE45CA" modified="2019-11-13" version="1">MPASQTSRISMSSSIVKKWIEEDGPDMSSQKLLPLSAVVIPASPSTTIDSEESFQCRGPNMTRSNGGLTPIPREPIVWASGIKGATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUJ0</accession>
    <name>A0A5B0MUJ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribosome bioproteinsis protein brx1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">BRX1_1</name>
        <name evidence="6" type="ORF">PGT21_010859</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUJ0"/>
    <dbReference type="OrthoDB" id="1638493at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026532">
        <property type="entry name" value="BRX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRX1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="5">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1079420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="71" mass="8015" checksum="F76FE4ACF10F208A" modified="2019-11-13" version="1">MDEVERTGNCLKGSRPIITFGKQFEDEVHWKVCKEVLTNVFAAPKTAWKAKRFIWSLVEASSSLDGDGSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVV1</accession>
    <name>A0A5B0MVV1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006581</name>
        <name evidence="2" type="ORF">PGTUg99_026856</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVV1"/>
    <dbReference type="OrthoDB" id="2507116at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137299" evidence="1">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="117" mass="12777" checksum="16FBA9AE6911A37B" modified="2019-11-13" version="1">MNCLFYAACFLALLQSTLAMPALTPRADQVAASHKGQSDEKCYFGCGFSNYGFYGSLFGSSYLTNYMSPYFYGNNLYGGLNYNLAYNSYGSNFGIGANVGLFKKDAPEANKHQENSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXS4</accession>
    <name>A0A5B0MXS4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016543</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXS4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="112" mass="12104" checksum="DC45CAD0B00D53C4" modified="2019-11-13" version="1">MATLKVYHRATKSKLSVGSPSSSSTLNLSSAISIADLPSNTNSTRFSANDPPSSHDPFNNLFSIDLFNSLLVLLAIPPSFSLRIRDILTLLLTSRSSVGLWSGLACQRAFDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0P8</accession>
    <name>A0A5B0N0P8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0P8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="7950" checksum="CBB1D5E7C33FD1CA" modified="2019-11-13" version="1">MTTKAVKRVLKAPIKLLLSVTTNCGLCDVGMKCRFLILCNLCQFNLPGLPESSVSNPRNEKLRNPDSCVRPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0S7</accession>
    <name>A0A5B0N0S7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine-protein kinase tel1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TEL1_4</name>
        <name evidence="2" type="ORF">PGT21_001919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0S7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021668">
        <property type="entry name" value="TAN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11640">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01342">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Telomere-length maintenance and DNA damage repair" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="208" mass="23208" checksum="B629166CF86EB813" modified="2019-11-13" version="1">MAPQESRWAEIFHQLFSAADTEKAAILKPSNARKGSIARASNLNRLRAALTLIRKCVVQFVSQFKHKVMKLVINRTIELIVVAKNRTLMDSIVLDCIRILRDFLLYRPHLEHLDHELSAEILAICFASILDRKLPETFSIPDNESFGAEPPLEAEPLNPTDRSQAASPRMIDSSQLLSIVIPASSSVCLECSKIPTVRLSQCIGKIPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2P0</accession>
    <name>A0A5B0N2P0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025338</name>
        <name evidence="3" type="ORF">PGTUg99_011670</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1123955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2P0"/>
    <dbReference type="OrthoDB" id="3226064at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="624" mass="71041" checksum="A0F8E7A5F8F15039" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3H3</accession>
    <name>A0A5B0N3H3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007947</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3H3"/>
    <dbReference type="OrthoDB" id="2496203at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="408" mass="46926" checksum="B2D3F43A69CFBF64" modified="2019-11-13" version="1">MKGVTLLPSQPPLLSTTYTTPMEDIKRLTIGKPDASANYHSILLAKLHSLYSKIKGYAAAFDVSRRPRTVPTYVDQLRTIHLPNLQEDIVRLNDILLCASKPTEDQSYEYRFRVALRALLDIEQTIHRIHALMSLLWTSEGTKTIVLDLTRFRSRFTEKRIAGLWKNLEHLFATYTKLFPLCGMLRQVDGTMLDSFRLRSMQSTNRARDGLKKLIDWLEIPDFRVLQHAWASTVNDFDKALKGMTESRQAETEDPEPIFQESYSDGIIVIKLLRIFMRKLSRVDNIELSQMPSTVHVSILKATAPLSRGLNSLIRDIDDADYFDGQDDHFHPEPAGPVAGTVTEIIKILNKYFKLQEASSTPLSHRNQFRDWSNMWNNHFEIAVERLHSSQQIAFDAFLRQMREPPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6G6</accession>
    <name>A0A5B0N6G6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_024734</name>
        <name evidence="9" type="ORF">PGTUg99_012825</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6G6"/>
    <dbReference type="OrthoDB" id="428177at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079:SF6">
        <property type="entry name" value="GH26 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="7">
        <location>
            <begin position="109"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="435"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1084332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1092282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="459" mass="51461" checksum="0481ECE1C2D5E4E2" modified="2019-11-13" version="1">MSVRSVLCLVWRSYTIHEYSKNLNCSGDWITTFLAAIHHISCHDNLNPLLSFFFSLSRIQPFKMKEFISGVLAATFLCSIVVSIQSSHLGPKFRQSSKQIRQAPKATKHDKRSINTGLSSTNLNVNGIAVGFLPALSEPIQPNTPLDINRRIGAPMSVMGSYIQLYAKDTNIAEIDWQLPQYRQLIGWPVWEIAIMPVEGLENVTPEIAGNIARKMQMLNAQGITVWLRFAHEMNGDWYVWGQKPALFLEKWKLVTAAVRSVAPKTLMLWAPNSGFSKDQDALLGGYTKYWPGGEWVDMIGLSFYHYGGHERANVLPSPTEAQDTIKDFVKLFGSKNHDRPVVLAETAASYTRSLDGKPAPGTANERDIKLSWMSQLLSRSMAEAVPELKAIVWFEVMKNENAAGNTPIKSEDFRIVTGQIKPLADDAYKLLSHRSSKSSKVRKPKRSDHLGKKRVGMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N879</accession>
    <name>A0A5B0N879_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N879"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="185" mass="20484" checksum="DEF882733AF6759A" modified="2019-11-13" version="1">MYSPRLAPRPVTSSQNASKSPRSKSPSQQANFGRMSNVLVIVHTDCQEVQHVLSIGHNNWTRRQRSLSVSFATMIETTILWIFITLLPFHRNGDTKNGASRHPRHFGRVVKAIDSNQLDSSSIGISRAGSNPAEDPKLILRSLRGGPTGLQTGPTLSLSHLGNPRGSLQRRHRPSLRRERVGATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9H4</accession>
    <name>A0A5B0N9H4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004205</name>
        <name evidence="3" type="ORF">PGTUg99_006737</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000250">
        <property type="protein sequence ID" value="KAA1118448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N9H4"/>
    <dbReference type="OrthoDB" id="2512050at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366237" evidence="1">
        <location>
            <begin position="26"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="619" mass="72993" checksum="7A230F0BBCA9440B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBX7</accession>
    <name>A0A5B0NBX7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">UNC-45/Cro1/She4 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_012907</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBX7"/>
    <dbReference type="OrthoDB" id="199930at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1086825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="701" mass="77739" checksum="C1ECCC3D845838D9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDC1</accession>
    <name>A0A5B0NDC1_PUCGR</name>
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        <recommendedName>
            <fullName evidence="9">DUF300-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_050057</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDC1"/>
    <dbReference type="OrthoDB" id="5348404at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="11"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="545"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="677"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="734"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="706"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="751"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="761"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="789"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1085749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="802" mass="89813" checksum="32321F63B611EDAB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFA2</accession>
    <name>A0A5B0NFA2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFA2"/>
    <dbReference type="OrthoDB" id="2505558at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF3">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="152"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="426" mass="47329" checksum="0B2CC76EC6974AB1" modified="2019-11-13" version="1">MPPRPSKRPIRPPKLPSRPSSPPPYPPANSSQPPPSVFVLATHHASPLDHIPMSGLHIQSAAAQQSSLLPPISEMDWSASQSLPPIRYAPPDPSQPSLQSAYAPSWDSNPSTQPPPLPLSGNPVPSAKKSHPIADPIDTDNQPEPNRSKTLQWTPSMEKAAIQLYSDAALEGKRSDNGFKSDVHQHVVNRLNEQFPGSDFTLSKCKSKLSQSFKKEYDAFVACRNASGFGWDDIRCEVTASNKVWENFLKARRFRNQPFPEWEQLQNIFGASASGDAAKSLSQRAKEGVVRGRPDEDSSSSDAVEKQDNQPRKTHTRKRIRQTTGAAFSSAIHGLIEAFAPQASSQNSTQPSDSLRKTDREVDQQVVNEAIDLFQSTFADQLPMDDLVVGFSVLENPSKARLYLKVEERYKNQWLSYEIQKHSHYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFE7</accession>
    <name>A0A5B0NFE7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFE7"/>
    <dbReference type="OrthoDB" id="2499852at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="252" mass="27673" checksum="F211317C82A83E46" modified="2019-11-13" version="1">MSDFPPCEANGSINLDTSFVELILDLLANNERMIQQLEATFNQLNTKVDPLIEQMAEMRTFMKTGTKPATNAGKSFASVASAALSDSIHAPTTRPSPKQVMASLKPKRVIIHSNPANTTLKDVPSGALVQKANEALLGLDARVEGEVLAISFYTKNRAHQKWLMENKHVWSKAVHPDLEATPSTWSVMAHGIPKSFDISKPANPALLASKNNFQSADLARVRANRQQRRPAPWSWRSPTKILPSELRSPASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFS6</accession>
    <name>A0A5B0NFS6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007581</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFS6"/>
    <dbReference type="OrthoDB" id="10452837at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="228" mass="24442" checksum="5E4BB1EAA5F613CB" modified="2019-11-13" version="1">MAVRNLAINPAPLSWPSLSSRHPPVPSSTESAAFVLHHTAKEAQPRLPRTYSSFTSDPHRYKTSSERISAQEMAFKNLVILAVLVSMMIGSAMPISYPTTRLSIRGTEQASSLSNGAEQAATASSGGQTKTDDSSTEMPKTQPEPPKTTPLDDRAPTESDESLHNAASKTQKPESPAGSSLSSNNNTSTVQEAFKPSKPTNTASSYLQSIKLQLVAYWSAICFFSLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJL5</accession>
    <name>A0A5B0NJL5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJL5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7740" checksum="28CBCD9BE2437165" modified="2019-11-13" version="1">MGPFSKFSEDPGRLVPRPGRFFATSTGTNLNKHKYVTPCMYALTESFTLTAMYVMTVDSLTDDRQRQGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NN54</accession>
    <name>A0A5B0NN54_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450-dit2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_011478</name>
        <name evidence="12" type="ORF">PGTUg99_032723</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NN54"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF1">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366262" description="Cytochrome P450-dit2" evidence="10">
        <location>
            <begin position="17"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="505"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1070408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1089986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="62861" checksum="CAC61F644779BF30" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNI4</accession>
    <name>A0A5B0NNI4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_015157</name>
        <name evidence="5" type="ORF">PGTUg99_018845</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNI4"/>
    <dbReference type="OrthoDB" id="10261640at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF8">
        <property type="entry name" value="ANGIO-ASSOCIATED MIGRATORY CELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="126"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="214"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="356"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="398"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1065420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1090835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="431" mass="47506" checksum="CF9B644313BCE75C" modified="2019-11-13" version="1">MEAIAENETSQPEMTDGDEFGHDEQHTEEEIEINEEDILETIDCQQVEGEVVMDEDENEEMDEEMGEPHEQSEPPFDSSVTRFTKHQEPVFCLATHPCNPQVCASGGADDLGYIWNASTGQEILKLEGHRDSLSAIEFSCDGQYLATGGVDATIKIWKNCDPQAFSKWEFLMDLEGSEEVTWLTWHPKLAVLLAGFSDGMIYMWLIPSGSMRVFAGHNSMTTCGNFTPDAQKILSATDSGMLFIWDPKTGKPLHKINLNEGKYAFRAQQDQSGINSLVINQANTICVLGGIANGGVRLVNLKMGTVIAALDEHESHATVEVEIFEPEGLGVPLLISAGTDGTAFFYDAASFKLRNSIRHQDAITTLVIDRATGQMTTGSLDKTLSTWNLKTAEQLKVHSGHRDVVHMAKMTSDHKKLLSCSDDGNVLVFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUB3</accession>
    <name>A0A5B0NUB3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUB3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="121" mass="13663" checksum="C324A6A001ECAAA3" modified="2019-11-13" version="1">MSSLGHSKVTDQTPIFIDSSPAISPLAEEMEISMLLTPPGYSIYDGDCFTQPKFDALFAFVMNQITIYCRDNYPKNFLEHEARVKLYMDISRGFKFYEDGMPHGSSSGGNHTLRRILQVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUB4</accession>
    <name>A0A5B0NUB4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_017795</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUB4"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16988">
        <property type="entry name" value="ANTH_N_YAP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000004">
        <property type="entry name" value="ENTH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="4">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="291"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="466"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="595"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="744"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="479"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="493"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="596"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="763"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1092907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="808" mass="86614" checksum="375216AF0F101E6C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVK9</accession>
    <name>A0A5B0NVK9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CTLH/CRA C-terminal to LisH motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVK9"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH/CRA C-terminal to LisH motif" evidence="2">
        <location>
            <begin position="80"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="170" mass="18182" checksum="F03E1EC3200D5FEC" modified="2019-11-13" version="1">MSEHAVRRLGGSSPTSHSCGGASNGMATDLSNSVGALSNDTSTTSSSVSTQTTSLALSFPHCRALHDFVHQLPEPVKRAIFAKELENVAGLLAYVDPWDSPVRKYLNQSRRDLLAHQVNAAILVHLGAGTLSSSSGGNPPDELVHCPAHREETLPMSWYTVQLIGRKPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NW87</accession>
    <name>A0A5B0NW87_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NW87"/>
    <dbReference type="OrthoDB" id="2496582at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="500" mass="56799" checksum="064B9F2AC0C85FD6" modified="2019-11-13" version="1">MDINAGLDEIHHIDGLINPKPSSWVYYCLLAFAIFKAFMIFICLGVIIIPIYLNGVGSRKHLWLIRRTHVDSSSIPVLVPNRSLVVALLELVGSTMFFFHICLNYLATNHPQVTLTKAVIWDAIGGLPSWLSIWLTGWSLLHTCQGRRHGSSNTNARFPPPWAYNITWISGCFLAIVLAAYACNKFSLMSSELGLDLRNLHEMMMQRSGLRADPEIQTLASEMQDEIQNFHAELAAQKKVLTGWVTGWMAFGIVLVLFYIFILQLLVRKIRKVLRLCEAEHFRFLSQTCDQALHNEKLKIVEKSLAPLHALRQELRLLMFSSFSIMMVLLAEVSICACRYWSLKTEHLSVFMLEFSLISAMIPSVFMSPVLLIQCWKGLISHAPIHDPPERAARQQKGLTDGMEMGRINKAGWSCLMDKHTAETSSQNSTKFQLPAFYQRLFWWYANPDTDDVDCLHIQSNYPPSTVQLSTASLVSVDPECSRLELRSIQLSEVPDSHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P048</accession>
    <name>A0A5B0P048_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016333</name>
        <name evidence="2" type="ORF">PGTUg99_013430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P048"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="84" mass="9735" checksum="DF45C2B3CA351A21" modified="2019-11-13" version="1">MKVQNKLPQVIEQHLKDVTNLLAEHPMQNNPRTLENQIKALEAKQKEHMVKLEAQQIELEAQLEAQLEVQQIELQAQLEAYQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8V4</accession>
    <name>A0A5B0P8V4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007034</name>
        <name evidence="3" type="ORF">PGTUg99_032104</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8V4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366368" description="TNFR-Cys domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16183" checksum="53990564ED67C723" modified="2019-11-13" version="1">MHYITPIILMSIGQFLDILLGAGGLVGAMTPHHQINCNSPYLTFPRLTAKCDSCQLHTKVTNLVSCTSCRKSSLVYEECSTKGCPANWHKSTCQEHKFKRGILHSRCENCQQHKKETQTISCKNCKNSASTFPYCSSPECHSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P961</accession>
    <name>A0A5B0P961_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013100</name>
        <name evidence="3" type="ORF">PGTUg99_015503</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P961"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="165" mass="18681" checksum="DBC1C02AA8C04C00" modified="2019-11-13" version="1">MAEVDIASHDLMTKETSRTEPKQESTSHTLSNQPVTNSSHHLTNLSLSFINYKPGTPNHQNDTLEPPVDSPRSLIRLRYLWTPPPPELRTASHLRGLILKTYPSTFRYADRIPSLLAETPPKPPPVVILDLGSYPRGHRKHPHNLGPSLILLYPKYYTKPPHHPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBN2</accession>
    <name>A0A5B0PBN2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036100</name>
        <name evidence="3" type="ORF">PGTUg99_021396</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBN2"/>
    <dbReference type="OrthoDB" id="2506573at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="592"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="845"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="606"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="850"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="930" mass="104147" checksum="3C76379B3D47C5F4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJH1</accession>
    <name>A0A5B0PJH1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJH1"/>
    <dbReference type="OrthoDB" id="10526438at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023038372" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="9516" checksum="58FEDDE3A5289556" modified="2019-11-13" version="1">MHAIKSIFILLTLKFALVSACDVNYRHTCESHDDRPGPRESPIVLRATDDPCRWYETSMCCDAAKLKPGEIKRGREVSGIFCTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMM5</accession>
    <name>A0A5B0PMM5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_033530</name>
        <name evidence="8" type="ORF">PGT21_033700</name>
        <name evidence="9" type="ORF">PGTUg99_025096</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM170 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000070">
        <property type="protein sequence ID" value="KAA1133849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PMM5"/>
    <dbReference type="OrthoDB" id="2131401at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019334">
        <property type="entry name" value="TMEM170A/B/YPR153W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779:SF6">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10190">
        <property type="entry name" value="Tmemb_170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1098325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1101930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1133849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="224" mass="25016" checksum="7AB19F04E873F034" modified="2019-11-13" version="1">MSTISHLLFKRASFSLPSTFASPPPGYVTPPWPSLYGPNSDGIYLYVPSTIWKFTLYWTLLLEGIVFMGCGLFASFTFWRSGSLREMNGSKNGKMGKMRIGGKTMAKQARIRRSGSVGRTENQGEWIPLNRHRRASSIITTIHHPVENLNRTRRPFTSLFLTPTFFFLIFSFSAVISGSLVGWAIAALYNAAFLRMSTWVPALWSAGLTLIVIISSYSNISTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNF9</accession>
    <name>A0A5B0PNF9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNF9"/>
    <dbReference type="OrthoDB" id="10351839at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="86" mass="9221" checksum="3567FCF55AE164DD" modified="2019-11-13" version="1">MCLSAYSFGRNQAIHPQMPKKPHHSSSSPAQVSASLASPSFRGSLREVPPARRGPPVPLERGDCAKLARSQVCWEPGIAPDPPYAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQZ1</accession>
    <name>A0A5B0PQZ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQZ1"/>
    <dbReference type="OrthoDB" id="2504241at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="870" mass="98929" checksum="7C026C88ABF36CFB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSX8</accession>
    <name>A0A5B0PSX8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSX8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="59" mass="6458" checksum="9D3B8588B9B13C36" modified="2019-11-13" version="1">MALTLPIPAFPNPLQQLILILKFLWLCNPCTLFNSVFLSVIGKITNALRPSHKAAIALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT40</accession>
    <name>A0A5B0PT40_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000424</name>
        <name evidence="2" type="ORF">PGT21_036927</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT40"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="164" mass="18447" checksum="C8BE2C2EB4142363" modified="2019-11-13" version="1">MTLVSTSLINTVQISNTPGINKAHELRVTGLRFHGPRAQGSMAPGQDPPAHSAQEIAQVSILSQTRPLEPILNPKQSPRRYNTQPHLQPYLDTAHPQSDALPSRQSTYVQKLQVSLFLIFRSNNWSLQYVSPVVTPSNQLSPQFRDQQYPVEGHPNRLYLGFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTM6</accession>
    <name>A0A5B0PTM6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prolyl 4-hydroxylase alpha subunit Fe(2+) 2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002962</name>
        <name evidence="4" type="ORF">PGTUg99_016540</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000312">
        <property type="protein sequence ID" value="KAA1105137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTM6"/>
    <dbReference type="OrthoDB" id="27483at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099:SF7">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prolyl 4-hydroxylase alpha subunit Fe(2+) 2OG dioxygenase" evidence="2">
        <location>
            <begin position="171"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1105137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="954" mass="106775" checksum="2E408DB1EE9B29F2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXL7</accession>
    <name>A0A5B0PXL7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXL7"/>
    <dbReference type="OrthoDB" id="2506739at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="732"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="862" mass="96091" checksum="24D453F50D54B03B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0L3</accession>
    <name>A0A5B0Q0L3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035896</name>
        <name evidence="3" type="ORF">PGTUg99_023324</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0L3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366509" evidence="1">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="85" mass="9441" checksum="85B494EEB5985A59" modified="2019-11-13" version="1">MHISMKYLCILLQVLCFTQVAHAMNQPLKGGAEASISQMKPDIQSARIKTEYGYNPMASQINVKSTAHVAQGTLKYQPLEIYLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q236</accession>
    <name>A0A5B0Q236_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_003712</name>
        <name evidence="8" type="ORF">PGTUg99_008283</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000010">
        <property type="protein sequence ID" value="KAA1137234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q236"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969:SF71">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_5, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474493" description="RING-type domain-containing protein" evidence="5">
        <location>
            <begin position="27"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="65"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1107139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1137234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="180" mass="19734" checksum="C6F627CDBCFE8592" modified="2019-11-13" version="1">MSIGITHSLNFQLLQVVVLGTPTIHAADQIGLPIPVGPKSLLKPVHQHTKRMNLVENNQYSSEMCTICRDELGAKSYRWPTCLHLFHFRCIDEWRSGTGAQSAECPNCKSADPILARQKNPQNNSQGGESSSGIDSPSYATCFVCSSRTMHPVAGQRVTGTGRIQLICSQCRHSVTVRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q390</accession>
    <name>A0A5B0Q390_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018207</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q390"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="108" mass="12072" checksum="831CC32B18AD5561" modified="2019-11-13" version="1">MSDTPGSPAEMHSPLHIRLREIGLDSKFQLLSSEQLQRVVAWSNPDLRINGSLNTTINHASCSSFPSTPPQCHQRWASYANRSVSFLSYATQKLLLAKKFGPLTLQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4N1</accession>
    <name>A0A5B0Q4N1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4N1"/>
    <dbReference type="OrthoDB" id="2505424at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="200" mass="23017" checksum="F85989CEBD545430" modified="2019-11-13" version="1">MEPKAAAEPSTILALITRWFNGRRDSIRKEERKPGSAETQKRLVQSSRRRKTLAKHRSDTLEMMKVPEKFWGIFEDPLCNSDTESLEDGTLVKVKLKWRSELASSLANKVDQISIRRKKEDNRRAFGPGQLLETRRQHSMQNIQPNKNTKVPRGLAVDFYDDQFLEGLGEQARYEMGVESSLGLSDLCFHLEKYSFNSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q577</accession>
    <name>A0A5B0Q577_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SGS1_22</name>
        <name evidence="11" type="ORF">PGT21_009502</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q577"/>
    <dbReference type="OrthoDB" id="2499463at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0347">Helicase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="51"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="263"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="646"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="718"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1108356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="755" mass="85677" checksum="4C4444BF9FF03D71" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5C0</accession>
    <name>A0A5B0Q5C0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5C0"/>
    <dbReference type="OrthoDB" id="10460928at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="155" mass="17549" checksum="861D1E1D2E3B0786" modified="2019-11-13" version="1">MNSRPTLVSATTERLQVFRVGNNITGSRTSRRDPFTASIVMKFLSCYSNYSTFPDTLFSQTDLSLTLFPEGMKSIRRKQLGAIGQRSTSYLSIPDGGPLKFGFDVMKYLECNGCGLYTLLVHWKLLRNSKRKSSSNSKRILVFDCHLRGRAYLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7E1</accession>
    <name>A0A5B0Q7E1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q7E1"/>
    <dbReference type="OrthoDB" id="10533842at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="362" mass="37714" checksum="5C3618E536C0DA76" modified="2019-11-13" version="1">MRLTQFGQADQERGSCEADDDEGLAASRNPHCRLLDFGKWSPMASDIKDAYLVGLRKYPDIFPIHIFSNIRLLNNRLTSTSTLSTLNWQEPLNFALLEPSGLLEIPDAHIPTAFTCSHNQIANRTLFVKTSNMQITLLSIVAAMMLSAPTASARRGGHARRYGGGYGGGYGGGYGGGLGYGGGLGYGGGLGYGGGLGYGGGLGYGGGLGYGGGLGYGGGLGYGGGLGGGYGLYRRQLNLNEEQPSQEGAGTNQGDFGSNQQGPRFNQEGSGSTQEGSGFGQEGLEPVGERAFQGGNDSEGQQMATEGAFPEDSSTERTLSDDSSDDQESGDDQSANQDSSSDEEQPTGAPEQAEDTNTQEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9X3</accession>
    <name>A0A5B0Q9X3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_004825</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9X3"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF83">
        <property type="entry name" value="K+ POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="456"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="513"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="10">
        <location>
            <begin position="30"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="10">
        <location>
            <begin position="128"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="11">
        <location>
            <begin position="741"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1109961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="914" mass="100236" checksum="A59BD0D58F1664EB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA77</accession>
    <name>A0A5B0QA77_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Uracil-DNA glycosylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_011583</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QA77"/>
    <dbReference type="OrthoDB" id="565731at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008263">
        <property type="term" value="F:pyrimidine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10028">
        <property type="entry name" value="UDG-F2_TDG_MUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015637">
        <property type="entry name" value="MUG/TDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159">
        <property type="entry name" value="G/T AND G/U MISMATCH-SPECIFIC DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159:SF9">
        <property type="entry name" value="G_T MISMATCH-SPECIFIC THYMINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="4">
        <location>
            <begin position="166"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1110130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="407" mass="45352" checksum="776D3A18414E0705" modified="2019-11-13" version="1">MKGQNPEPRGLYKPLAYPTASYSSCLHWKISIVRPTKSEYTYDLRDRTSEALAGPVDMSPGTLQSKYFASKASAKSKDDGSSGCHFSGNRTERVVTRALAKKRNRSLSAPSVSASIACEKDTCDDQQKVMISKHFKPGLPPHTTRKKKKARQYAAPAVYQHLNHLPDLIAPSLDVLFCGSNPGCQSAKISHYYAHRSNHFWKSLFLSGFTPHRLSPHDDHLLPSEFGLGLTDLVCRPTAEANELSGSERLQGVPVLLGKIRRFQPRFLCFVGIKQYHDLCSYLKKKLRKSRDLLPSIWQSPSQKTVVGLQNVVFRSQKTSRINKGQSKSTAADADQYSHTFVFVCPSTSALVRNYPEKDRVACFRELLELKNRASLNPDSSATPIASGSSRTKLCLEYIEIDDALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBB7</accession>
    <name>A0A5B0QBB7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_023148</name>
        <name evidence="3" type="ORF">PGT21_035178</name>
        <name evidence="2" type="ORF">PGTUg99_008237</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000514">
        <property type="protein sequence ID" value="KAA1064199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBB7"/>
    <dbReference type="OrthoDB" id="2511112at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1110487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="74" mass="8026" checksum="5D164E539C6D7F5A" modified="2019-11-13" version="1">MIFHFSAVVRHDVGIAAPTGGHLSGHAWPGHISAIHSNGHPGLPLDPPDVEDKLRSQNHPPDRFLPVKFARQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBS2</accession>
    <name>A0A5B0QBS2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBS2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="588"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="701" mass="77799" checksum="83113B45CFBDC80E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBX1</accession>
    <name>A0A5B0QBX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBX1"/>
    <dbReference type="OrthoDB" id="2499550at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="343" mass="38052" checksum="C56B2616D4E3F465" modified="2019-11-13" version="1">MSLNSSIGLSRKPSQRLLSAVRSIKQKVNRPKGQPKDANSLGTRPLSETILDINFQGGSSTSTTANCRPTCWPMLAPMMFEFEILPRFYRAAPYSSSHQETDTARSVPYGQNVQSTNVNHRNAQEKRNVGVFRVCQVSPTLSRCLPSTPPLGFGKLGDQNKPEQPLPRLFCPPPRCDRTDSLVQPPCSPYRSSEDSSSSQESQESDYASRVSIRLSVEIVQSFFDHTGPSSPCSESSDEELYAEKISARLENLCYDKPLEFIHRRSHAGKGNANKRISSFGVARIIISSPNALQKPLLSPSFKSWLDKFIESRLNQDESLNGFHGSFESMIPEETHCYSGGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDE3</accession>
    <name>A0A5B0QDE3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_037080</name>
        <name evidence="3" type="ORF">PGTUg99_002409</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000020">
        <property type="protein sequence ID" value="KAA1136840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QDE3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1136840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="129" mass="14727" checksum="559D04F18AA3082D" modified="2019-11-13" version="1">MPPFRSKDFWQKFNRNILMLDLLKRKLQNDATIIEVAQPFSRTMQDGELLAIEFISHLAGFCQHNNKEEKPQIVSSKETSPEALIDAQSKLPKDEDNGIPAETRPLLSPPIKPESEPETEHTTISMEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEV4</accession>
    <name>A0A5B0QEV4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hook C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_001382</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEV4"/>
    <dbReference type="OrthoDB" id="49395at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22211">
        <property type="entry name" value="HkD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000217">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008636">
        <property type="entry name" value="Hook_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043936">
        <property type="entry name" value="HOOK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947:SF28">
        <property type="entry name" value="GIRDIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947">
        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05622">
        <property type="entry name" value="HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19047">
        <property type="entry name" value="HOOK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116907">
        <property type="entry name" value="Hook domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HOOK N-terminal" evidence="7">
        <location>
            <begin position="34"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Hook C-terminal" evidence="6">
        <location>
            <begin position="248"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="263"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="482"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="895"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="545"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="634"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="837"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="263"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1111483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="974" mass="112177" checksum="17019E06FE8078ED" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFG5</accession>
    <name>A0A5B0QFG5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009687</name>
        <name evidence="3" type="ORF">PGTUg99_008840</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000136">
        <property type="protein sequence ID" value="KAA1129462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFG5"/>
    <dbReference type="OrthoDB" id="10270155at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366519" evidence="1">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="92" mass="9781" checksum="A56528BCB2F15443" modified="2019-11-13" version="1">MMHSLQLIGAFMVSFSLIGSLVASSPEVKVECGNARVLNLKAPNRVCKSGTTFYDMIASVPCKPKEHSLCCVRRVDSGSFIPLGCTSMGGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHU2</accession>
    <name>A0A5B0QHU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHU2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="116" mass="13467" checksum="6F30914FCE60CB02" modified="2019-11-13" version="1">MLMITGPDEDLNLGFQLRLVRLARYFNLRWTPSPLNLDRAATPLIHLSVSNLPRHLALVPSLASITPHHSVPITTQPTRHTVNPSDRQRLSTSSDYDPSHKWICHRYRHFQFIRCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QII6</accession>
    <name>A0A5B0QII6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ATG9_2</name>
        <name evidence="21" type="ORF">PGT21_018972</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(out)</text>
            <dbReference type="Rhea" id="RHEA:67920"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 19">Preautophagosomal structure membrane</location>
            <topology evidence="3 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19">Belongs to the ATG9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QII6"/>
    <dbReference type="OrthoDB" id="2020634at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF10">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0072">Autophagy</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 19" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="614"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="652"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="723"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="58"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="220"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="782"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="910"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1023"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="107"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="925"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="943"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1059"/>
            <end position="1069"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU364027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1113027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1069" mass="122022" checksum="1ED4DE4CD8D8F1C2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QKZ4</accession>
    <name>A0A5B0QKZ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024666</name>
        <name evidence="3" type="ORF">PGTUg99_018540</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QKZ4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="120" mass="13003" checksum="5E2C0D772DEEBC49" modified="2019-11-13" version="1">MQGSEGGLRRLQQIGSVCTGQQRIRPLPNSTVDQEGILLVNEVVQPRRPVPPVPARLYNLADKEDSLLVDGTTLPAQEGILLVDEAVQPRRPVPPGRRQPNQSLLAGDNLAGQEDSLLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMY5</accession>
    <name>A0A5B0QMY5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMY5"/>
    <dbReference type="OrthoDB" id="10269984at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10647" checksum="9A997F97EC94BB46" modified="2019-11-13" version="1">MHISLLLKILMNYTISNSGDFGCNLHRHTKPSKWATASRGKSSVDIVSLSKGGQAHHIRLKLDTKAAFRENEVPILDIVTVYLEGKVSCPFKRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNK1</accession>
    <name>A0A5B0QNK1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">KU70_2</name>
        <name evidence="18" type="ORF">PGT21_019225</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ku70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNK1"/>
    <dbReference type="OrthoDB" id="761538at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00788">
        <property type="entry name" value="KU70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000001">
        <property type="entry name" value="X-ray repair cross complementing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047087">
        <property type="entry name" value="KU70_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005160">
        <property type="entry name" value="Ku_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF2">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03730">
        <property type="entry name" value="Ku_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Ku" evidence="17">
        <location>
            <begin position="24"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="343"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1114710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="362" mass="41166" checksum="4406400EF5375717" modified="2019-11-13" version="1">MHHSNESRLYLLAEPKPGRSQDSTAVVEKKDIVNYFPIGDMKEIKTFRRVIFTNEEIEKMRTVGLDKGLTLLGFRPRDELLWAYHVKHAYYIYPNEDTFVGSTRAFSALLHSMAKKNKIGYGLLRSRKNDTPTLVAILPQLEDFDEASNTQMEPPGMHLCVLPWADDLNPPPTVNQLDCLKQDESSNHITELAEKIVRKLRVDYAGNKIHNPALQHHYDFLAAKYLNEKFEPAEDESLPWYPSIKERCGPLIKELVEEIDLDPRAAESVIPESSITKKRRRIEDGEGVDVEVVESAFASGKEKTLTVILLKEYLIFKKELSIAVKAPLKAELLDMTRKFLAKQQNKSASGSTSLGTSRKKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNU6</accession>
    <name>A0A5B0QNU6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNU6"/>
    <dbReference type="OrthoDB" id="124998at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150:SF5">
        <property type="entry name" value="OS07G0546750 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150">
        <property type="entry name" value="OS12G0169200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="109" mass="13314" checksum="DEF9216E829A841A" modified="2019-11-13" version="1">MSNHIGNRHRRMEELYDWDMEDEDEDSINIFKELETEEEEEKSRAQRRPNKERSHLEGHQRLLQDYFVQGSTYSDTDFKRRFQMRRELFLKIVVDVEAACPYFVQKPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QRJ9</accession>
    <name>A0A5B0QRJ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine-zipper-like transcriptional regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LZTR1_2</name>
        <name evidence="3" type="ORF">PGT21_000401</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000008">
        <property type="protein sequence ID" value="KAA1115820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QRJ9"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052124">
        <property type="entry name" value="Rab9_kelch_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647:SF1">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="364" mass="41161" checksum="564FFB276C22E482" modified="2019-11-13" version="1">MTEHNHIGQYYSNIDWTHHQLMYTFVTRHRIVKAFNKLQPPFPHTGASSAIHRSLINPPTMFFFGGSSIQNQHNDLYRFTFDPNDSKPSLTAGIVNTRGQAPAPRSHAILSATDGHLFLWGGQTNSNVLDHHLYRLSLSTNYWNHYIISGPSPALLFGSSSAIFEGFLYVYAGHNVNRSVQGDMWRIELDSLPSVPSWELISSSKDQICPPARTSHSAVVSSNSWIIFGGTDGVNVFNDLWEYRFREKNWRQVSANGSVPPPRKDHLVTQVKDHMYVFGGHDVSDHKIFDFFALDLINPCGKAVVAWDETIYVFGGLLSNNSRFYHTLNSISAWNQQLQHQNYISTSSMESLSSVEDYPTNEPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QS94</accession>
    <name>A0A5B0QS94_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell wall alpha-1,3-glucan synthase ags1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">AGS1_3</name>
        <name evidence="4" type="ORF">PGT21_000350</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000011">
        <property type="protein sequence ID" value="KAA1115813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QS94"/>
    <dbReference type="OrthoDB" id="512920at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047657">
        <property type="term" value="F:alpha-1,3-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070600">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058656">
        <property type="entry name" value="Mok11-13/Ags1-like_GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058657">
        <property type="entry name" value="Mok11-13/Ags1-like_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058658">
        <property type="entry name" value="Mok11-13/Ags1-like_Ig_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058655">
        <property type="entry name" value="Mok11-14/Ags1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47182:SF2">
        <property type="entry name" value="CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47182">
        <property type="entry name" value="CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26108">
        <property type="entry name" value="GH_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26114">
        <property type="entry name" value="Ig_2_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26111">
        <property type="entry name" value="Ig_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mok11-13/Ags1-like GH beta-sandwich" evidence="1">
        <location>
            <begin position="47"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Mok11-13/Ags1-like Ig-like beta-sandwich" evidence="2">
        <location>
            <begin position="181"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Mok11-13/Ags1-like second Ig-like beta-sandwich" evidence="3">
        <location>
            <begin position="310"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1115813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="430" mass="47641" checksum="5FDD2ACDD9CA11E8" modified="2019-11-13" version="1">MPLERALTGCQDEWNSLDHFDPTSPVRKVLSHFTHLRAQYPALQDGFNLAQRGNWTSFGQLPGSNMTQTEWGFWSVTRSPSDQQQFTGPNGNTTVWMLYSNLNTTKTFEFDCGTQLWISAPYPAPLTVRNLIYPYETYNLAGSKSPYYLDGKAPYRGCLQSVTMDALGFKVLVPADNWVAPLPQLVHFTPGHDARILSRSDTDSNPIAISLSFSDEMSCQSVSESLSLAYVIDPASSHQPRLNVNSATCTSIPPVPSSISSAPAAVWTWSSQIEDAPDGIYELIIKNPTNKAGLHTQSTDHLLIRKGSRDNPIAFQTTSYSKSLLQKGSDGLFQIFSNAAGADLMRYSTDFGKTWMKWQPYARAVGLPAGSFSQAQFWEGNHIRVQYWSKLAGSAAQTVDSDYGYSGTDIRTVPQLLLRGPFNQWLAEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2K5</accession>
    <name>A0A5B0R2K5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2K5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="157" mass="16948" checksum="6511B78921E0564B" modified="2019-11-13" version="1">MGSHGLFTPPPALQQFTISGGPDSILLHRHSAINSRCFGGQTSLIPLHSDIHIFTFVLASGLLRYTKLEMPFLIKSTALIFLAALFGRIQAKELCSLAKPRLWCSTARMSKTVHPLDEGKCKSPGARRYGPFLACCHPAVTPGKGGSIGCAVVHKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3A6</accession>
    <name>A0A5B0R3A6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_035750</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3A6"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="104"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1119879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="222" mass="24619" checksum="DB81D299C31BFACE" modified="2019-11-13" version="1">MNCLPDSSATDEFHELFLLAVRLHALENAKSAEDQQMNQNQISVPLPEMVQANNDSLSPQEGSIAAGIAVSVAPSPAEVPPQAGQLQNHLPESTTELPSNQTRFFCTHPGCDDSADTRFNLKRHMEIHTPVEERAPRILCEKQGCDSTFGRPADYKRHKIKFHPEMVEPGTGANQVSAKIYSKQPLGAFQCPEPGCKHRYQSETSGHFARHMRRVHGITIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3I0</accession>
    <name>A0A5B0R3I0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_037149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3I0"/>
    <dbReference type="OrthoDB" id="10627252at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1120096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="331" mass="38493" checksum="5B8D283F9AB7CD46" modified="2019-11-13" version="1">MSEFSSETSQTCSEAGRDINEFDSKPVLASHWSRFGPAIGGNSHDMVRPDYSKSEEEEKVLKVIATIRELRAKKSGLVIDEFRYPASVKGSQTLIGALLKGLKSIRENSPPFQKAFMETDHPKKEFLENLEKVDQLILIHGGSEFLAPTEGKELNLAHCKDTKSWKDMTKISSQGFLKERYQHMDECQCEMRKATTELMKQMSLLSDKLYRGLIEDPEFNLKFKTVLFQAMGLMYKHELITVQDLQDFFDRQKLRFAALHMNEQNLWSLTRGYQDLTEFWYPTLKKGVHDCWYYSYLKDIFQGKILEYKQKKTLSRHLLAMKASHYLNKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R5L6</accession>
    <name>A0A5B0R5L6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_037251</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R5L6"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050308">
        <property type="term" value="F:sugar-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000145">
        <property type="entry name" value="HAD family hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000023">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051806">
        <property type="entry name" value="HAD-like_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481">
        <property type="entry name" value="FRUCTOSE-1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481:SF2">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
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        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="2"/>
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            <begin position="1"/>
            <end position="19"/>
        </location>
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    <feature type="chain" id="PRO_5023101838" evidence="2">
        <location>
            <begin position="20"/>
            <end position="470"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="228"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="252"/>
            <end position="292"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="470" mass="51541" checksum="6CA4D3B45D7BCF42" modified="2019-11-13" version="1">MNCLLAWVFSRAIIQGTSCAYKTEALSERLTRESGKNKTRLLLTISRNFDTPLLKSSSPSPSIFYLSSDLLLKKLILFTPTQLITMSQFNLCQSLNETNQAEMVVDAILFDMDGTLVDSIAAVESAWGAVAQTLNKDPQEVIDATHGRRAIDNLKDLKPHLRRMSNAEMEPHVEAFETEILRQADHFQNEVRSRRSSVASSKRQSRETSRKNSMVINGHDHKPRRKSSLAHFITADGVSGRMAALGLKPLTQAPVNGHADHEDPNSSNIDEDVFDDDDEDEDEIKTDEEEVDVSDLTDKSVRILPGVRELIDSLPEGRYAVATSGAKTYCYGALKRVGITPPKVTITADDPRLKNGKPFPDPFILAAECLGYEPGNCLVIEDSPSGIKAGVASGAMTIAVCTSHPPEKISECGAHWLIPTLELVKVEVRKSDGKLRIYVDAEKDAVLKARKHAVQSSAPPELSKSIAPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RAS1</accession>
    <name>A0A5B0RAS1_PUCGR</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029423</name>
        <name evidence="3" type="ORF">PGTUg99_037610</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RAS1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="53" mass="5853" checksum="911DEC7C870DE630" modified="2019-11-13" version="1">MVPLTRSPIHTRATTLQGHNRSPSGSDDAPEATIRHERELPPHRELSSQSRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJQ2</accession>
    <name>A0A5B0RJQ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NGG1_1</name>
        <name evidence="7" type="synonym">NGG1_2</name>
        <name evidence="7" type="ORF">PGT21_017726</name>
        <name evidence="8" type="ORF">PGTUg99_021661</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NGG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000174">
        <property type="protein sequence ID" value="KAA1125927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019340">
        <property type="entry name" value="Histone_AcTrfase_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10198">
        <property type="entry name" value="Ada3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="681"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="746"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="877"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1020"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="258"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="298"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="407"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
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            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="500"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="633"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="691"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="900"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="918"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1071729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1125927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1114" mass="121768" checksum="501B45964BE29925" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S333</accession>
    <name>A0A5B0S333_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ras-protein Rab-18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_009484</name>
        <name evidence="9" type="ORF">PGTUg99_017296</name>
        <name evidence="8" type="ORF">PGTUg99_033674</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Lipid-anchor</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000271">
        <property type="protein sequence ID" value="KAA1116050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S333"/>
    <dbReference type="OMA" id="IRYCDDQ"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001918">
        <property type="entry name" value="Small GTP-binding protein Rab18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1080499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1116050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1131463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="26225" checksum="D602032E54CB73CC" modified="2019-11-13" version="1">MVSHPPQPGPSNSRASQAPDQRLSTEPPQTLKLLLIGSSSVGKSSLLLRFTDQVFLSAEESSATIGVDFKFKLIDHKGKRYKLSIWDTAGQERFRTLTSSYYRGAQGVLLVYDVTNRASFESLPSWFSELDTFAHSPQDVVRVIVGNKVDKDESRTVSTEEGRAFASDNHAMFFETSVKTRKGVDEVFEAVVDKIIDSISLQRARQGYRNDSRTLPGSIDLDAYSDDDQAGSWCNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJR1</accession>
    <name>A0A5B0LJR1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016439</name>
        <name evidence="3" type="ORF">PGTUg99_031922</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000506">
        <property type="protein sequence ID" value="KAA1068297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJR1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036136979" evidence="1">
        <location>
            <begin position="16"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="131" mass="14203" checksum="2899704F7E97AE47" modified="2019-11-13" version="1">MQGLAVLAYVVLVYTQLICVVLPSEVEVGSPPNKFCDICNWKAGYPQVPLPATQRFHKQYINVACGDLSGINCHLPIDVDAYYCQKCNHAAYYSVSGSCSSGHFQISPSYIQGPSLNGKQVDNPACSTTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LM17</accession>
    <name>A0A5B0LM17_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_018822</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LM17"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000034">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF85">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G00710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1064900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="456" mass="50825" checksum="4AAA25C026BE1B4A" modified="2019-11-13" version="1">MFYFLSFLDRSNLGNARIAGLQSSLHLSDHQYSIALTVTYIPYIMAEIPANLLLKKLGPNYFLPGIVTCWGLITTLQGFVRSYSGLIAARFFLGLVEGGMFPAIVLYLSFFYTRRELQLRIALFFSSASLSGAFSGLLAYGIIRLDGRGDLAGWSWIFIIEGLFTFFWGLISFFIFPASIESSKYLNPSEKAILKARLLRDRPMADDSLDCFSFAEVLAAIKSPHVLINFVSLFMSGTVLYSQAYFQPTIVNSLGYSASRSQLMSVPPFVAAFVVMLATAYISDKYCTRGLVTIGCALTGMIGFILFHVADQDQMSLKYGSLFISLSGVYSTIPPLSAWQSNNSECHYRRASSIALGFIASNLGGILSTWIFPQSEKPGYRTGTLLSLAFCAGMIATVSCNLVWLKSANRSKIVKREELLKDYPNHDQKSHPENKRIRQQAWADLGDRHPDFKYTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNK8</accession>
    <name>A0A5B0LNK8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNK8"/>
    <dbReference type="OrthoDB" id="10491561at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="58" mass="6873" checksum="49107F7C1D35EC2F" modified="2019-11-13" version="1">MKKTNQWIMNPSLKHKEAEAVGLENQKSMVLKEKETNLKRTKELEIQVESLDQQLCRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPT7</accession>
    <name>A0A5B0LPT7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011929</name>
        <name evidence="3" type="ORF">PGTUg99_009477</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000483">
        <property type="protein sequence ID" value="KAA1070899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPT7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="113" mass="12706" checksum="F9A4D8D0DC5F25E0" modified="2019-11-13" version="1">MIILLSRSETIWAAVLQRSISISSSPSPRRPPELLWSFCLIEIYPYRTPSKRSNYFGLTSIQASLNLFQAVATTITQSPALTPPSLQEMTQTKRKHKLRARKLNNPLPLGAPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQH6</accession>
    <name>A0A5B0LQH6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_012786</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQH6"/>
    <dbReference type="OrthoDB" id="2502344at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="4">
        <location>
            <begin position="282"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1065848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="445" mass="50263" checksum="09D05EB17C7C2249" modified="2019-11-13" version="1">MTEQKARINGMLHGRVARIVGSGQTTVPLNTVLPSLFKHLSTPSAHPQHPGTPSQTPTSFIMRGYSKRQSLIRDMFMIIVWMEHNELEDMEDAVILRRKPFPTDGEIAFPKNKLLQLMYDLLLSDQALFEEQLQIILRSRYLSLRPTPKTRDEFDLERLFNMPDIDFQQAARTTKAGFVGVLDIICMNPVFHRGGICPQLPIAHQLALTLERLGSNGNATSVGRFSRNLNVGCGTVIKATRWVIEVLVSLGRQYVMWPNSTRRAEISEVMKNEGFVGCVRFVDGTTIPLFQRPGYDGEVFYDRKRRYSINAQIICDCDKYITSFITGWPGTCRDSLVYKRMQLHLNSSQYFDDGQYLLADSAYEVSKTDVPAYKNPSAKIPINTDFNYCLAKASSPKQTYNWGPQVSMEFIAGDATAPLSSERHAGTCSLALQLHHSTQHPCKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQZ9</accession>
    <name>A0A5B0LQZ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027355</name>
        <name evidence="4" type="ORF">PGTUg99_021421</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000142">
        <property type="protein sequence ID" value="KAA1128402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQZ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137063" evidence="2">
        <location>
            <begin position="22"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1066273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1128402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="232" mass="26084" checksum="D126710D5241F369" modified="2019-11-13" version="1">MNISHMSLIVGALVALGYSESKMNSSIEGGIHIEDHLDQTNSIIDGSESTSSHLSQTSTDIDAELYDYRVAYNDYLNEHQINPVQIRQSQSFSTRIQDWFKRLAFATGIKYLRKKTRTVGYMPEKYASGLSHKMKPNLILSISGAKRILKDDITVFKKPHYVHGASSSKTDPGRSKTDPEVLTSKDPTSLSFNDDSESSTSDQLPLQKITKVKLVAESQIKDEERERMRVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRZ4</accession>
    <name>A0A5B0LRZ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021143</name>
        <name evidence="2" type="ORF">PGTUg99_003358</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRZ4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137051" evidence="1">
        <location>
            <begin position="20"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="112" mass="12260" checksum="8ACB407F5686FA7D" modified="2019-11-13" version="1">MKCSATAWLWAALMGAALGNLVTAPVRCPACRCTNTRVTSSTGPSQCRRWFTCALKVVHYRCTSMGVTTNDHACNDCDNEWTSCNCVFEVMKHRFANCPTSGVHPWLGQAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSD2</accession>
    <name>A0A5B0LSD2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008958</name>
        <name evidence="2" type="ORF">PGTUg99_027757</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1067029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSD2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="72" mass="7414" checksum="2A488BFA5A15DAAD" modified="2019-11-13" version="1">MLVAPLANDWPSAYSVEGVLTKISRSGTGTAQRQPMQILGVSHPTVGPGGETSSPGGWPATTKRNPLIGESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LU90</accession>
    <name>A0A5B0LU90_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014131</name>
        <name evidence="3" type="ORF">PGTUg99_029028</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1067676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2496852at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="87" mass="10149" checksum="CC68294C9729D69B" modified="2019-11-13" version="1">MYDPMLRVTQKYIDLALKCDTVVIATFLHPSWRMMLFNSRFKTHVPQITKLIQKVFDPREAEVDSLQPESPPQKQNQLPSRSTSKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUS0</accession>
    <name>A0A5B0LUS0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_29</name>
        <name evidence="3" type="synonym">GET3_95</name>
        <name evidence="2" type="ORF">PGT21_000526</name>
        <name evidence="3" type="ORF">PGT21_032705</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUS0"/>
    <dbReference type="OrthoDB" id="2509730at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="825" mass="88343" checksum="D69F2687DC69D3F9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYC5</accession>
    <name>A0A5B0LYC5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYC5"/>
    <dbReference type="OrthoDB" id="10471431at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="536" mass="56858" checksum="94125DD454624B35" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZ83</accession>
    <name>A0A5B0LZ83_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZ83"/>
    <dbReference type="OrthoDB" id="2508271at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="328" mass="36620" checksum="3038964FF85720BF" modified="2019-11-13" version="1">MNPTSFPLRSTGRGTRDETEYPAIPENFEQMPGEADRAMTEIPEDVEEYAKRMMRETMEEMLRGPGYMQLAKARSLHMSPEMMAAFQPSSTTSRDESNFTTQSQLPLPSEAFKRSMREQVEILTTYPHVVDLAKSTVLGLTPSTPLYTVTEGFHYSPSHPHLRVPNIIPVHQCNCSNCAGLQHQNHSPLPSFAPQGTSIHKPESRCLCTLHVDSNPGQSSRSNSHEPRNPGYMGIGNVEEGTMVASYPNWSGAPRELQQDTNASQSSRFNPHELRYPQEIGIGNVEEASMYASYPSGSGAPRELQQGEQVRRSQGRSAPRDLSGKWYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZJ6</accession>
    <name>A0A5B0LZJ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZJ6"/>
    <dbReference type="OrthoDB" id="10301892at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="240" mass="26452" checksum="1B4C31353ACC1274" modified="2019-11-13" version="1">MKAQELDSHPALKPKEQAHVKSLLALIKADIENSDDEGDDERRAESDATQLANNLPPSSASHESTHRVTTIPAVQSLHGDAPISHPKLDLSLPARPNFDTPTPMHEDRQYGLFRSMAVPKVLARTPFPSFGKFSRVDKTPVVDNGFPNVLNPAYADIPDNSPGRKNSRGETSYLTEEGDRFLDSVMASVNQNQNLTPAQGNPVPPVPTPPADKQPPPAIPKRQSERPVVIRERQERQPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7L6</accession>
    <name>A0A5B0M7L6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7L6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="134" mass="14433" checksum="8029F7C9B1268C52" modified="2019-11-13" version="1">MDDYREMDNSHWSADLNAMNLNGGGGRSRANSRTEMHGEDFLRSARSQLGNDFKTSSNMGSMRSRSNSHVGLSNNEGLLPNPCRDLACRRSPGGTTPSELASHSEGGYPRVSARIPANAGGYPPADADVDVCFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2024-01-24" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8N7</accession>
    <name>A0A5B0M8N7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032744</name>
        <name evidence="3" type="ORF">PGTUg99_019061</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8N7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="121" mass="13859" checksum="488EF3C4FAD00CD4" modified="2019-11-13" version="1">MQDGSLVTNNLNVQGGNFLFPDENFGLNFAGFDGIVEMVWKADRFSHCTEISTPLQSYNSCLGVSCEIPLTSLQTITWLQNLYYESKKEAFFRDTNKVIEDCQAWKEKQAQKAQKIPRKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDU6</accession>
    <name>A0A5B0MDU6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDU6"/>
    <dbReference type="OrthoDB" id="2506601at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="362" mass="39226" checksum="31CAFB225FED2CE4" modified="2019-11-13" version="1">MMSARSSTWSESAASELDPLFLSLKEPAGTPRRTRSRPSLNPLPSPSTSSDSPLPIPATVNFPLPPRRLRPGELIGIPDPAPTSSISPADRPDPFLAPVSLYGVAIGGNNPIKTNPAVNGRAEVKTAGLTSLDLRKMSLRNRSSASISHPPCPPPETISLRARNPSRMLAPHTPSLHSPPRSYSHNTQQQHPPRYQQAFSASTSNLITTPSVERSVRSSSRPSPQSSLPPRNLSLVETSSNPIPRLPPTRLAYQSLSYNLSTTPEPFDHSLSSIRQPLSESLSSDLNNFNPNPQLRRHPLLSASPIPPQNASDSLAIYSGMAPITDRSNGRIKFAPPSKQTRLCGTLCYPFPEPFRITEYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKM3</accession>
    <name>A0A5B0MKM3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019846</name>
        <name evidence="3" type="ORF">PGTUg99_034413</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKM3"/>
    <dbReference type="OrthoDB" id="2517701at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="263" mass="28776" checksum="E06D65360EF0EF01" modified="2019-11-13" version="1">MTIVGKKVAAFFTKWSSSPATPPSHPQLNQFCPMSNNTARWPPSDYSMASQITFGSLPPWPLGSQDSPNPGATGTPTASTATNHTSLVGSTSLSNQIFPQGPDTSTPAGQTAELFRRVLTTPSTTSLSVPSNPVPTTSVNVQNHRSPTPEVDLTRFQGRQKQSKLSSETMEQVNQQSLDQLRRTVAKDPEYLRLTVSDKLQLDAAYRAYQSAVHRIAIENKLHIKPVLDYLGNEARIRGPSCYNNFCKYDPVAGAINRDSKWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MM79</accession>
    <name>A0A5B0MM79_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MM79"/>
    <dbReference type="OrthoDB" id="10501868at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="147" mass="15667" checksum="50AD4815385F6CC6" modified="2019-11-13" version="1">MKPDLSIKSEVTNLPATSIAKAAASEETIKSSAPDPVVKSEETDSHAASAAELSDPEETINVHRGVEDHVHAPPSLNKIYDLAIESMARISPCALHPFNRYLETARSPLPENFTFLSGNGTAASPIRVDTPLPAGVELQRCWPVAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPE3</accession>
    <name>A0A5B0MPE3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021744</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPE3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="57" mass="6623" checksum="ACBE5B2D5DC21503" modified="2019-11-13" version="1">MGWLIVRMADWVHPKNRPRSLMPKSPPAQLQFILTIQISHLSEFKYIFGRMDGGVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPI2</accession>
    <name>A0A5B0MPI2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPI2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11978" checksum="4A9C50E82AC16F2E" modified="2019-11-13" version="1">MSLDGPNGFRPHRHPDERNALLIRLQCSVGRDKSLLIKRLDRQRISSSIKVRGNLKNARHDLELKDEPKGLQTYQTLFIQSYEKERNYINPTKNRTPQALGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWA0</accession>
    <name>A0A5B0MWA0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWA0"/>
    <dbReference type="OrthoDB" id="2504593at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="240" mass="27611" checksum="A1446D583EDAED85" modified="2019-11-13" version="1">MALDDKFWNDGLYFHSKAPWAVDPDVRAGITCVLVLSRIQEEFQLIAQELARAVGWAIAHHAHLVEYIGYLHKRYDRLNQKDEHGKCLHGAEEERLVPFDCVDQMDLGRVNRRLKIKLVEQEMFTRLNEHKVLVEEWSEDVVWLWSMCQPIPNKPHIQQWHELINQIRTRKVRRDNGVGIDETLEDTVIEVGALDGDGKDASDEMVNENEVHEDTVNEVGALDGDGEDASDEMVNGNEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWK6</accession>
    <name>A0A5B0MWK6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014861</name>
        <name evidence="4" type="ORF">PGTUg99_031899</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWK6"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="41"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="517"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="537"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="623" mass="66882" checksum="7F490DC319ADC957" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWV1</accession>
    <name>A0A5B0MWV1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA polymerase III subunit C82</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">RPC82_4</name>
        <name evidence="2" type="ORF">PGT21_013054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
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    <dbReference type="OrthoDB" id="272392at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="142"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="191" mass="21274" checksum="9A08696EE6FD6D91" modified="2019-11-13" version="1">MQHSIASGRFEIDPAQVSIAHSRCDIAPKAIKTRTKHGQDKSLRCNIALSRSEVGLTGDATLPFPDEIFTWVRFGQFANSAYKQFGIESKLIVRYLLDNGKLRVGQLVTMFSTNLFLLSNEVSPMASRTAISPGLLSSGEDLESLEEVKQLLQEQRQVIQNLQDQLEHQEMDIILLKKLLKGSNSAAPESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXY1</accession>
    <name>A0A5B0MXY1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH CENPB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_018413</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXY1"/>
    <dbReference type="OrthoDB" id="2507562at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00674">
        <property type="entry name" value="CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="295"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
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            <begin position="423"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="630"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1080729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="677" mass="74943" checksum="6F24CE3DC6482BE8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZY4</accession>
    <name>A0A5B0MZY4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007029</name>
        <name evidence="1" type="ORF">PGT21_032921</name>
        <name evidence="3" type="ORF">PGTUg99_020243</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZY4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="51" mass="5716" checksum="E03102582023AD10" modified="2019-11-13" version="1">MLRRKASLTHLLGPAEGMFETSKPVPPVRFQLFMGFLSPLDSDDFGDIGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N757</accession>
    <name>A0A5B0N757_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030201</name>
        <name evidence="2" type="ORF">PGTUg99_024688</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000342">
        <property type="protein sequence ID" value="KAA1099242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N757"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="150" mass="17091" checksum="FCF67C348630F73B" modified="2019-11-13" version="1">MDIFHFTLRSQHHSKIEIEIEIQTQIEIEIEIQTQIEIEIEMWTEIEIEMSKSISSSPANNQSQNLNHQQLISEQLQLTWNKLGQPRPQDKFDWNQTLPNLMTEQKACIAGMSDGQVARTVGSGQTTVSLIVSRAWTRGTVKTAEKSGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAG7</accession>
    <name>A0A5B0NAG7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">V-ATPase V1 sector subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">VMA4_1</name>
        <name evidence="4" type="ORF">PGT21_008005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NAG7"/>
    <dbReference type="OMA" id="QHMMAFI"/>
    <dbReference type="OrthoDB" id="10263003at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1085464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="226" mass="26076" checksum="0B4E89A642762026" modified="2019-11-13" version="1">MSKPMTDSEVISEMKKLVAFIKQEAMEKAREIKVKADEEFSIEKAKIVRQETTSIDALHAKRRKQVEVQKRITQSTLSNKARLQQLQMRDQLLQDVFEEAKKGLSDLTTDQDKYRGILENLTLQALFSLMAKDIVVSVRPQDRELAEPAISNAIQRFKEESKIDCTLTVNEDLSKDSKGGVVVWGFRSRIKVDNTLDERLRLLEEKMLPEIRTTLYGKNPNRKHET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NC69</accession>
    <name>A0A5B0NC69_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011132</name>
        <name evidence="4" type="ORF">PGTUg99_013526</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1135967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NC69"/>
    <dbReference type="OrthoDB" id="2507589at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137436" evidence="2">
        <location>
            <begin position="23"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="170" mass="15901" checksum="EDF52B438D281AF0" modified="2019-11-13" version="1">MFSLQFSSLITITMSMMALSNAAPMPQLGLSNFNGGNQYGSSQYGTNGFGNNGFNSGYNGGQAESVGQSSSNSFQQSSGTSTQSSGASNEVFGSGGASGYGANGFGSNGASNFGSGYNRGSSALGGGLGGGLGGGLGGGLGGGLPGGVGIPGGIGGIGGGYGGPRGIGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND41</accession>
    <name>A0A5B0ND41_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_024794</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND41"/>
    <dbReference type="OrthoDB" id="5392716at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="215"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1087187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="505" mass="57521" checksum="9F0084C0E39F11AD" modified="2019-11-13" version="1">MPKKSSHNSKILPHPPKNSNIATPSAKFNPFFTNPNPTISLMQSPISPNQEFEEDVLSEYSTISYPDRSDFPEVLSELDIKEAIASLVSQGVTGKEIHKFLQGDCGVKISLRTLNRKRAEWGLQNCDLPKPPKNSKLTPDVEASLISSHQQGFKVSEIRSRLLSDTGKDVSHRTVERYLAILDLKLKRNDLADGKVTRDDVVCLIDHARKELLATTHGPNHIWSADGHDKLKPFGITIYGFIDAWSRRILGMYAHVTNNDPLHVGVYFLQLVADTGGVPLKVTVDRGTETGEMGTHMIELTQRYTGITFEEAQTHMHHTKSTRNQKIESLWSRMMKEHNRPLIDTIWTQIEEGKYDRDDDIQKLLFLFLWMPVVQKSVDRWVTIYNASRKRKDKLTELPTSCSPNFAYSTPEHFGATDQLIHVPQEDTNFILENTYPEREAMFTHTPDWFHDLASLIMQEMGFHFDQMAPGGVWVIFDEMLPYIQANIPSDYIPNSSFNNSATQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDV7</accession>
    <name>A0A5B0NDV7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015421</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDV7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023061825" evidence="1">
        <location>
            <begin position="21"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="171" mass="18896" checksum="78039879993A8A58" modified="2019-11-13" version="1">MHSPTFLIVVLLAVLSGLRALPHRWYEVPVPGELTEPLLRPGAMELPDEEMIQEATPVETVLPVPPLQPKELWLRMVKKHLPDVPNPEAHTFKLVSNHQLCSAVIQSTCLPCPGVVNGDSFKCIKCGGLTAVHWNPCPYNHKTGIYDGAVGREKDVDEEEEAIQQIVGLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDY2</accession>
    <name>A0A5B0NDY2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chitin-binding type-4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033578</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDY2"/>
    <dbReference type="OrthoDB" id="160054at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35396">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35396:SF1">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022790099" description="Chitin-binding type-4 domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="444"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="50313" checksum="1386B72E895F457E" modified="2019-11-13" version="1">MLSLTGLVAAIAMAGLMQLPYIAGNADPATGHIRNVKPTQAWLDKNQPTRMTREIQASECAMNTRLSYPHVQLFAWFKVNHGEDNYHGCPYGDCWAYATHPGPDDVEFDFLDSYTFFWHGLAGVEGTGVKEISDPKSGALGYEQSLSGKFLVGPTPMGTLQVDHDEHYPGFDPSKLVPWPKGAVNRFLWGPPEPKHLKCGRPCEENRDPGIAPGAYGSKMFSPAPASAYPPPPSWVPGPEDKCGKNGTIPVPDKCASFCSCASHCKDDNCISQCGSKGNKCDKSKCPALPPPPANPPQPGHDSDKCLNYCSCLEHCGTEQTCIKSNCKKIKHGCENDKCNSNSNPPPSFNPAPPTTDPTTPPSNSGGSKNPGQAPKKEKKKPKKKKQDKSGNGSDGTGSDGTGSGNTGSGNTGSGNTGSGSTGSVGTGSGSTGSSGTGSGGSSGNDSNGNDSSGSDVGGGKGHSESGSRRVYRQTVKTKACL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFX1</accession>
    <name>A0A5B0NFX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFX1"/>
    <dbReference type="OrthoDB" id="2495058at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1087484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="11372" checksum="420BC853F837075A" modified="2019-11-13" version="1">MSGQINISAPAKTYITNRHCLQIHPLPPPIVGHLTGLTCPECPINYTSQLQYHPPRFPNPAKINWQCHKPNSHHRNGKGYIRTSGAYLKAWNSRLFECLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI89</accession>
    <name>A0A5B0NI89_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI89"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="316" mass="35303" checksum="2445CE3E7337DB62" modified="2019-11-13" version="1">MSHRSQGIGNYMESNRLRAIGRQALPSQNAHFKRASMYKRSPLLHKVKSLLRPKLNLSPSLFTLRVCVSANDRDERHRWNQGHSFYSSPSTRGLTHLAPTQFTLVPPPNQIMRTPRHTVCSHQQSIEGPSAAKSAQQVNDNRSSIQSPISNNQTHLRDIKKPSFAHPIRQGLNDEFKHHNTSFLPLPPVLHNRTLPHCSIQQFHPVLQGAVGSSYFSGHSHQPHLPSQHSLPPTPLPPTPYSNCQPISRLKVNINPFAAPNHDEHQRGPSTGLPSQSTLSFFDSSTSSFGENHMVPHHRSKTCKIHQSHVVPLSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIQ3</accession>
    <name>A0A5B0NIQ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009827</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIQ3"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008988">
        <property type="term" value="F:rRNA (adenine-N6-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25152" checksum="47697E802DC07047" modified="2019-11-13" version="1">MGSIRLKELEAELQGLRGFNAPKIELEQYVTSAHLASRMMFTAQSTYEDVLDKRVLDLGCGCGILSIASTLLGSAYTLGVDLDPDALLVARNNLASLDISDSTIDFIQADLSSPSSFRDLFGGHSRNDSEEEPFFDTVVMNPPFGTKNKGIDIVFLEIACQMSNSAVYSLHKSSTRKFIEKKANEYGFEGEVIAEMRYDLPKTMKIHKHKTLDIAVDLWRFQRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQP9</accession>
    <name>A0A5B0NQP9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HPt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036579</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQP9"/>
    <dbReference type="OrthoDB" id="2508290at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="15034" checksum="8324B98E5ADB811A" modified="2019-11-13" version="1">MGDIIDLHLFAELVRLDEKDEQPFLDDRISNYFYPSVKCIYAMMDDLRSGDYHKLEQEAFELRSLASSLAVVRVAQLCSFIENKCRSGINERDHIEIDSTLRVMELANQFAQDWLVKELYARRERRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQU6</accession>
    <name>A0A5B0NQU6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Structural maintenance of chromosomes protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SMC5_1</name>
        <name evidence="7" type="synonym">SMC5_2</name>
        <name evidence="8" type="ORF">PGT21_035837</name>
        <name evidence="7" type="ORF">PGTUg99_037135</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. SMC5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQU6"/>
    <dbReference type="OrthoDB" id="10254973at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="6">
        <location>
            <begin position="133"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="420"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="744"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="893"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="993"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1076205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1091575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1196" mass="136550" checksum="B4AE3457E2E0179C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUG9</accession>
    <name>A0A5B0NUG9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ceramide synthase 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CERS6_2</name>
        <name evidence="13" type="ORF">PGT21_001469</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingosine N-acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUG9"/>
    <dbReference type="OrthoDB" id="2498637at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF11">
        <property type="entry name" value="CERAMIDE SYNTHASE LAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="12">
        <location>
            <begin position="188"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="32"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1092402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="433" mass="51370" checksum="AC2093AD9A04DA74" modified="2019-11-13" version="1">MDQSQSSANPSTKTLRTRTSSVQLVLNHVTHTPPDHRFAANNFDHSTNLTPPNSSPTQTKSQAKKSKPIPHWEQVSQGFWHDLKTMRWMRHPGTSFRMILIILLPWAFLEQFRFLGWIDSNPLTSFVLISYRLPLQADESYHRYGKGTKDFLFLAFYIIVFSCIRQTVTEYLIRPYARYLALKGTKQDRFVDQGYAVFYWGSATLIGMRVMASQPTWWYNTAEFWKGYPHWRMEPTTKVYYLLQFSYWLQQMLIASLRIEKPRSDFVELIIHHLVTLWLVGWSYVTNLTMIGTTIFVSMDISDTFLGLSKCINYTKYQRTSEVSFAIFLCIWTYFRHWQNIRILYSVYAEYETLVPEFAKRWSPSNEVYLTGWMRWQIFAPIMLLQILNLFWYFLIWRIVYRMVVGNPLADVREEGETDDDDEDDSATKKKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWF1</accession>
    <name>A0A5B0NWF1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_014837</name>
        <name evidence="8" type="ORF">PGTUg99_005584</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWF1"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF27">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1084029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1093263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="183" mass="19954" checksum="AADDDA551B7DAE11" modified="2019-11-13" version="1">MDHNGMAGMSGMSMDQMTRPKPEHSGMGMMMNTFFIAGNPTVPLWFDGWTPSSGGATFGACFGLFVLTVAVKLLGALRHQAHLAWSMSKWHDSGLTTHQTIDKTNDHATPQSGSLRPTKPAVPWSAQRDIPRGILAALHVGLEYFLMLAVMTFNVYFFVAIVLGHFVGEVAFGRWSTVQGAHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYV6</accession>
    <name>A0A5B0NYV6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NYV6"/>
    <dbReference type="OrthoDB" id="409122at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S53" evidence="2">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="141" mass="15137" checksum="375712B5C44E6405" modified="2019-11-13" version="1">MSCGCSLPIFKLTTLESPEWQAKCSGPLTFRYLGPNKYKGFYNRTGRAYPDVSAQGSRYAIAWRQTFLSVDGTSASAPTFASIIALLNDYSISLGGPTLGYLNPWLYASGYSGLNDITFGSSGGCNTSGFKATKAGIREQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1I0</accession>
    <name>A0A5B0P1I0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028896</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1I0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="107" mass="12261" checksum="E7ED23C0B842975E" modified="2019-11-13" version="1">MFPNTSGDTEQKKACDQSQVVVTWVEPEGVSFEHYRGRNGIAIPISKILYYLEATVSMVLLSKSRLRVLVDSFFFFLLLAIRTLALHDEEPVRSSDKSQLDRIWLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P522</accession>
    <name>A0A5B0P522_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011525</name>
        <name evidence="2" type="ORF">PGT21_035122</name>
        <name evidence="4" type="ORF">PGTUg99_007819</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P522"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1121324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="167" mass="18981" checksum="88B8DF9686716C36" modified="2019-11-13" version="1">MVTDDSRPSTKQYILAPALRISRKLDSPSPTTPFHQVRPSNAQRSVANPTYLSVSETARVMEWTARPLLRRRRSWRHSTQRSGVHLSLFRAPERNASGHVQRLGCQPPRRSREPTTRWVPQDRPEGVRASRSAPPKQKRTPECALEGVRPKTRARHDLEPNTSASVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5S5</accession>
    <name>A0A5B0P5S5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ecp2 effector protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031979</name>
        <name evidence="4" type="ORF">PGTUg99_010725</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5S5"/>
    <dbReference type="OrthoDB" id="2503597at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366348" description="Ecp2 effector protein domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1107920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="29303" checksum="C87DD92E09716F21" modified="2019-11-13" version="1">MFLTHLAIALATAATSLSNVEALSDRSESAMRPMSLKTIHDANNMALLERYQMGRPGAQMKATVEIYAFKSSATLSKRNNIGEVPGITKPLDMTPSTCTSQVCYPGSFEAPKKADCDVIVDAQLYHSIGSLRSKPGQYVLVYAGTCVVVFQHPMGKGDNKLNLEYNWASLGKIILGIQKQCNKPDALSVGGVCKIDHYLKYNFENVLISLQRYVAPENPQTPMKSGQPGKPANSGKSANSDKSAKSDKSPKSDKPAKQDKSGKPQTPAPQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8M2</accession>
    <name>A0A5B0P8M2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023879</name>
        <name evidence="3" type="ORF">PGTUg99_036070</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8M2"/>
    <dbReference type="OrthoDB" id="10580199at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="138" mass="15209" checksum="F414843D0FFE38C3" modified="2019-11-13" version="1">MPSDSVVLPDASALLMPPPTLATTALPLPAHPSPVAQSQTRNKSKSSNRSTHRGSSRPSKYKKKNNTEDLYIMSIISKRQAEVTRARAEASKVKVSYMKELREHGLSLEEIERKAALEFPPCADMDDTLSNENSENSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA38</accession>
    <name>A0A5B0PA38_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA38"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="162" mass="17795" checksum="18F49F21F308E8D3" modified="2019-11-13" version="1">MLLKSSDPPLATSSSTDLENSSQNNNLGSLQRKAISSTSLTSSHLKKFVSRIDARKKDAVTKAALEDKKANAAGILGGNLGLENYAKFITDKNVREPHLIWLALLQHFQSNSSQNQVVVYQDFLKIGFNSTLEQFIKDIDVGISNICLVGIKIVKFEKSTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAA8</accession>
    <name>A0A5B0PAA8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031645</name>
        <name evidence="3" type="ORF">PGTUg99_018150</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAA8"/>
    <dbReference type="OrthoDB" id="2496180at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137715" evidence="1">
        <location>
            <begin position="26"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="511" mass="58465" checksum="1234FDA5E077BAF6" modified="2019-11-13" version="1">MVNDTKTAVIFNFALLYLCIQHCSGRPRPYEAPIPVNRLSSISQQVESLASHERPATGLFIPVSHGGSVFISSHIDESQYGRVWGGHDLHSSFSDLLKAMEGSNHDGFSQAHQPPVNHWHHTPVSSLPSHGVFKSTNWNTKVGSHHAPFKEINPREKLTNKLDIPFDVSNDITDKPFGWINTLNFPSNQKMGALGRDDWGLTSSSVWKIAKMELNDPALPSLLKKADNDDNQNPLTNLLALIKEIDIRNKQFLLAFSPSASGDEKYFMADLIKDRNYENMKDVHQRLLAWLRLQLDTTSQANSRAGLQNPNHEDSLTIFQKTLLDCLKSDLESKELKCSRWQVQLRPTKNQSTTVSLGQASLTKVAINAIGNFYKSSNLNKWKELFKFDHHFVNFYLILKSRQHHGSSKKRATESANWAQLQILPWENENGQQWVLDPAIHQKFSNSLKKFKLSLIEHKFLPVKESSEKEELQAMRYEEALREQKATRKRIYYHNRMARLKKARLESNKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAC4</accession>
    <name>A0A5B0PAC4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012879</name>
        <name evidence="4" type="ORF">PGTUg99_016825</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAC4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137716" description="Secreted protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1134021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="7927" checksum="1DB13BFD2CC0B4F7" modified="2019-11-13" version="1">MRNCCKIGLIIALFHLKVLSAAFTDLHVLADTALADARNNLIENHSACTNDEEEQMKKTTSFDLDGQSASSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAU2</accession>
    <name>A0A5B0PAU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000063">
        <property type="protein sequence ID" value="KAA1098727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAU2"/>
    <dbReference type="OrthoDB" id="2379842at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="222" mass="24861" checksum="E3CB831B0CF66E33" modified="2019-11-13" version="1">MVKKLTIRISQPSTNQRSIVKKSTIQIALPRPYQWLMVEIATKNPANGQKINHLDFPSTTHQWLTVKIVTQYPVDGQKIDHLDFPSTTHQWSTVESSTQNPEPPPNLSVREDGLAQKRKDLQAQLSSLNPIEENTKLEQISRVLDGSAAIVNIKKPKTNPKSHAKPKPQASSKKRKLEDLSPPSRNLSQLTGSYSQSITILNPQNHLSHPKEISSNPHCKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PB28</accession>
    <name>A0A5B0PB28_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphatidylglycerophosphatase GEP4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031246</name>
        <name evidence="3" type="ORF">PGTUg99_020483</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PB28"/>
    <dbReference type="OrthoDB" id="198652at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027706">
        <property type="entry name" value="PGP_Pase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF25">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROPHOSPHATASE GEP4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09419">
        <property type="entry name" value="PGP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137731" description="Phosphatidylglycerophosphatase GEP4, mitochondrial" evidence="1">
        <location>
            <begin position="23"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="45703" checksum="71A1F60DA435CBB0" modified="2019-11-13" version="1">MPNLNLTAIVASLRCLLSPSSLVPSLHVRDIRCVDWKELKRKGYIGVVIDKDNCITKPYHDQLVPELQHAWQSCLATFGNLGVLLVSNSAGTADDPALIQAESVARHLGVPVLVHATKKPGQQVVKAIEKYFTKDYRLVPVIYPSRRSSCGPPVSSRCHSNRTPLKLVVIGDRVTTDIILASRLRSHLQRNQSTPTSSSSTDSNPIFSVLTQKIWQKEKPGTRFMRWAENRALKLVMQSSTRSSTLIRGESSSPSSRLPNLIAFPKQLSTTFEQSLSSSSQAFSKAQQAKKHLQKKYDSISVRLKQISEQPLSTIIRSVLVNIQGMLANQLAKFLTGLGRRWIEFKITLQQHLTRILENLKTKALRALIQSANKLQLKLKPFNLFDPASLGFKTPLFIQNLSKWKQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGQ8</accession>
    <name>A0A5B0PGQ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGQ8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="91" mass="10632" checksum="D16D07C09702054B" modified="2019-11-13" version="1">MSVLFSAQNPELHSKFSGDLFTIYKILDETILIACYFNLFTHVGKFPSRIMSHVHCYKRGLSNKMSMLAFNLFVEKIRDKECPYADLMFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PH43</accession>
    <name>A0A5B0PH43_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PH43"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="411" mass="47279" checksum="1E0B94CC5A76E0B1" modified="2019-11-13" version="1">MWAKMGQKAPPSPTKLPWATPEAFHSLVITIFAVIKASRTLKKLPSYHPAPKINLSERSQLSSFNFEDPQDPIGLLLVSPSVPVNLQENAELIQRYLIILRLRKEEKERNYQPTPWEKLSEEEQLLLATYHAHIHQEEDLDRQIQFRSFLREIREKNNQLSRKRYLEKMSTFETGVIRQERASPTPSVDTDNGSDSQTSEQQTAQNYEVPSPLQRDLAAAKELQSRVENLKMTTIIRKENTATSQDKETSKEKQPLNKEKSSAMEIDEIDPECSEVTPTRPSTPEIRILSKEDQVKLRVKEHVGLWKQCLVASREGPTARLRALLTTAQESQKALQKLIDNEEIESYVKGWNPWEEKKIHFPSPPKKNLMKFKRNEPGKKQSSSSTNFSDPAKWKKVTDLLQMASGLYQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIA6</accession>
    <name>A0A5B0PIA6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Glutamate synthase [NADH]</fullName>
            <ecNumber evidence="19">1.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">GLT1_6</name>
        <name evidence="27" type="ORF">PGT21_017921</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Forms L-glutamate from L-glutamine and 2-oxoglutarate. Represents an alternative pathway to L-glutamate dehydrogenase for the biosynthesis of L-glutamate. Participates with glutamine synthetase in ammonia assimilation processes. The enzyme is specific for NADH, L-glutamine and 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>2 L-glutamate + NAD(+) = L-glutamine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="24">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Energy metabolism; nitrogen metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Nitrogen metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.14" evidence="19"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4327079at2759"/>
    <dbReference type="UniPathway" id="UPA00045"/>
    <dbReference type="UniPathway" id="UPA00634">
        <property type="reaction ID" value="UER00690"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016040">
        <property type="term" value="F:glutamate synthase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019676">
        <property type="term" value="P:ammonia assimilation cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097054">
        <property type="term" value="P:L-glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00982">
        <property type="entry name" value="gltB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00713">
        <property type="entry name" value="GltS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000006">
        <property type="entry name" value="Glutamate synthase (NADPH/NADH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000031">
        <property type="entry name" value="Glutamate synthase 1 [NADH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000017">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000113">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000022">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.60:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012220">
        <property type="entry name" value="Glu_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006005">
        <property type="entry name" value="Glut_synth_ssu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051394">
        <property type="entry name" value="Glutamate_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01317">
        <property type="entry name" value="GOGAT_sm_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008730">
        <property type="entry name" value="PRK11750.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000187">
        <property type="entry name" value="GOGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 24" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0288">FMN</keyword>
    <keyword evidence="17" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="13" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="16 24" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="26">
        <location>
            <begin position="50"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="23">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1173"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1179"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1184"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00057049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00068518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KAA1101375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="2131" mass="233994" checksum="29D8BD1129395CD1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJ28</accession>
    <name>A0A5B0PJ28_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJ28"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="80" mass="8857" checksum="A42EE6D5BC59157A" modified="2019-11-13" version="1">MLSFATEVASNRDMVEEKIDNEGGVGMRVASPLSHCADRREERPCGAALRALTQRLVLGSWHKSSRNHAIRSKATFLTTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJJ3</accession>
    <name>A0A5B0PJJ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_017458</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJJ3"/>
    <dbReference type="OrthoDB" id="406631at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000251">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF39">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="10">
        <location>
            <begin position="189"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="475"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1101351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="589" mass="66902" checksum="73F94F4A8911A3E4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJR4</accession>
    <name>A0A5B0PJR4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJR4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="110" mass="11162" checksum="35DA2274AAD464FF" modified="2019-11-13" version="1">MNGALIASSADDQPVGSEEQAPDFPDISTPARFADEGGGFSVPDVDEGAGDGGGSDKIPTGSDGETGERGSMSDEDGRGRVWRMLLTIGGGPVRDSSRGGGGKMIGFEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKR8</accession>
    <name>A0A5B0PKR8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKR8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF22">
        <property type="entry name" value="TRANSPOSON TY3-I GAG POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="688" mass="75326" checksum="9C07AFEED9013DA1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PM42</accession>
    <name>A0A5B0PM42_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_021801</name>
        <name evidence="3" type="ORF">PGT21_028450</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PM42"/>
    <dbReference type="OrthoDB" id="2506743at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990334">
        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001100">
        <property type="term" value="P:negative regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034586">
        <property type="entry name" value="Bfa1/Byr4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140:SF1">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="219" mass="23384" checksum="067914157D38E405" modified="2019-11-13" version="1">MQHDESFSSTSSECQSTIKASFLPLPTPDSQSSLNHHHQPPKNHSKRSVAQINTTVLAVLASGTKGTVTRLEPSKKPNPVQAGLDWDEDIVLPEGGLQTIFRPIVAFALAIAFPTWALSLNLAGVSGITGRSYSQDTQNIIIWALVSGTFFFGLSLSLLIGQHGYPIALFLLSIQAGLAITISISILIFSNGLTIHHTLSRSIFLAIGPSISGLITIIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMA0</accession>
    <name>A0A5B0PMA0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PMA0"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009141">
        <property type="term" value="P:nucleoside triphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.180:FF:000003">
        <property type="entry name" value="Type I phosphodiesterase/nucleotide pyrophosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="602" mass="68510" checksum="3B3B067D5288007B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PPN1</accession>
    <name>A0A5B0PPN1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009516</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PPN1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="104" mass="10899" checksum="1A8DE26490EF00A7" modified="2019-11-13" version="1">MVRYPGSSTLRLGVPAAGISFPAVGGVGMRVASPLLHCADRRQRPVGAALRALTQRLVLGSGFGIGFGRSGIRSDAENPQTSVLTWNPRIYPGRGPLCTLSLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRL5</accession>
    <name>A0A5B0PRL5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRL5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="157" mass="17712" checksum="FB059A237E4D0E2A" modified="2019-11-13" version="1">MLDYSPSYNPHPPRQEGDTIQLSHIVRVFLPMISIIIIIPIYHPIVVSISQTIDPTPHAAIIIIPLQIIMSHTKTAIIRQIRLDTITIIPEWTILILSNILHSTFQPAVIMKVGSNQIMCYIKNIPLEPPLQVKPPSKPIFQAIRVTAVLPNARKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSF1</accession>
    <name>A0A5B0PSF1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSF1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="116" mass="12756" checksum="4437061297C266AB" modified="2019-11-13" version="1">MPALRKKNYHDEAQSLKVEHLWNASLSNCFVGTTPLPGSQAEASDETFTVITWNTMPKPYQIQVLAATVLSDPDRSRSGLCAGLHSCIYVLSAAPLFGHNPTEWLLSPFTRPAVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTB7</accession>
    <name>A0A5B0PTB7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004432</name>
        <name evidence="2" type="ORF">PGTUg99_020957</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTB7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366471" evidence="1">
        <location>
            <begin position="24"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="636" mass="74664" checksum="90F80A979593B66D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTN0</accession>
    <name>A0A5B0PTN0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_002874</name>
        <name evidence="11" type="ORF">PGTUg99_016120</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000312">
        <property type="protein sequence ID" value="KAA1105135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTN0"/>
    <dbReference type="OrthoDB" id="1854502at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13292">
        <property type="entry name" value="PH_Osh1p_Osh2p_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000033">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000017">
        <property type="entry name" value="Oxysterol-binding protein homolog C2F12.05c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF205">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="371"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="500"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="600"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="735"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="514"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="604"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="747"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1092441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1105135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1261" mass="139937" checksum="97FF99BC6340915A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXR7</accession>
    <name>A0A5B0PXR7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXR7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022959649" evidence="1">
        <location>
            <begin position="23"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="158" mass="17411" checksum="BA90CF36A8B745C8" modified="2019-11-13" version="1">MQFPGALSLMTLLVSFAIQTRAVYPPPNDYLMSDKVDWANGYLSVYSANGKDAFRYGRDKNQPNSGTNRHRLMDGSGNQLFEVYSANEGCIYDWEYNSMSKARVTGNTTNSTKTKPTKAVGFIRRSGVKLINLKAYSEVNTAALGRAILAMSMSSPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q008</accession>
    <name>A0A5B0Q008_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033721</name>
        <name evidence="3" type="ORF">PGTUg99_032417</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q008"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366451" evidence="1">
        <location>
            <begin position="24"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="123" mass="13754" checksum="DD06DDC84E16FAF3" modified="2019-11-13" version="1">MHSLATLVSLGLVCTLFTGLALSYESSAPNIEARMCPECEHRGQRPITTETLMLQPKDYRFTCGKLRGKPYECDQVIAAKVYHCNTCNHWALFLKGRCTEALHNLAQPYFLPNEDFQGVSGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1F8</accession>
    <name>A0A5B0Q1F8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000459</name>
        <name evidence="1" type="ORF">PGTUg99_009629</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000308">
        <property type="protein sequence ID" value="KAA1106923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000019">
        <property type="protein sequence ID" value="KAA1111275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1F8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1106923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="54" mass="5549" checksum="738E313A9C28B1AB" modified="2019-11-13" version="1">MNDLAAVLTLCTTRRKNKKAKGLAASEGGNVSLRVPSGPSSSLISLLGVATYCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1W0</accession>
    <name>A0A5B0Q1W0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GCM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050130</name>
        <name evidence="3" type="ORF">PGTUg99_050217</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000147">
        <property type="protein sequence ID" value="KAA1127889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1W0"/>
    <dbReference type="OrthoDB" id="2496644at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1002"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1071"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1002"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1127889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1132" mass="126482" checksum="827CDC52B3D0A2E7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q232</accession>
    <name>A0A5B0Q232_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q232"/>
    <dbReference type="OrthoDB" id="2499328at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="498" mass="57778" checksum="B81B86FCD8FEC888" modified="2019-11-13" version="1">MEHQHIGSGLEKTKIAASEHDLSTHHEKALENLAQKQADYDSMTKLLDWTNREIRIVFATQILENAPELHVDKSGIETLKEIDEELTVVANAALSIYGPPKTPPEKSLLLKSSSQLSHPSLMNTVKVYMEGIPRLFELLYTPATLRPYYSYVGLASKSCILKMFDYLSKYKMMPTDLEDGFRMTMKSPSGLEWIAKEMQGAFLPGSKYGLKFHVPLNEAEFLENHPHLSQLANLYKELGEKEQNYVLFHSLKLSMSELYDYLFSVQKATSGPIPIQGTWHMNFLEKMEKILLKEFEPKDSHANSVDLDFSEVQQEILNCRKFLVDPAALSHNPEVQHHLMRYSFLILNFMDGKLGRNYVEKLGLKVQEHDRVEYQTAYEFMKSTGEVNVWKNILMDYGWTLATDKLFNPRVNEEDWEEKGAFYWTKFQEAANHYASLSRSLESDPQQTQLLKTNFYIQWNKAAWDSDLAEINRYYEDFRKLVQLDRIQAHNIPESYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2W8</accession>
    <name>A0A5B0Q2W8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006987</name>
        <name evidence="4" type="ORF">PGT21_014644</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2W8"/>
    <dbReference type="OrthoDB" id="2511868at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="126"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1107459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="184" mass="21753" checksum="3C2B76F031C281EA" modified="2019-11-13" version="1">MARINKRHVNRSSKMANTSNKQRARIQEHARLQEHVIGLHERILKSIRWQRLKYYEDSHLYPTVPWDEDTDHPLRGPTPQEVSNACKLVDSNYFLMKKGRPVLRDPSDHDSIIAIIEFTPWDKLTDAEKEDLDFLSTFLHKSKEFINPVSSSSRSWGGKMWAIGWRKSQDFLQMPDWQDPWRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q3I1</accession>
    <name>A0A5B0Q3I1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q3I1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="245" mass="28128" checksum="7A443A365A88A0D5" modified="2019-11-13" version="1">MRFSIFFARPTNRLRSQISLDGGSPSFALKFPPSTPVLSRRKPSPLGKNTSQNRSNFSRFIVAKENYEEFGFPRAIRTTNIANYLLISFSGGLFENKIIDTARLLFRWTMTSSLIMFEEATTSCPFCHYLIAVRSSRLNHQQYPKHSKVTNQTPIFIDSSPAISPLAEEMEISMLLTPPGYSIYDGDCFTQPEFDALFAFVMNQITIYCRDNYPKNFLEHEARVKLYMDISRGFKFANVHNLIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4I8</accession>
    <name>A0A5B0Q4I8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4I8"/>
    <dbReference type="OrthoDB" id="2507458at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="133" mass="14643" checksum="5BA00E4587A0F4AB" modified="2019-11-13" version="1">MIVTPVTLVRTAPGRQAAVQRVALRGQENARVVRLPPSGPLATPSPEPAPIIPPAHHHPTSVRPLAFRRRPQPEGTSTAYGHPYRPENHHNAGSNGSQQPRSPAVYCHRYSTHRQPLHPQHHQVLYPPPVSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5J9</accession>
    <name>A0A5B0Q5J9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_008677</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5J9"/>
    <dbReference type="OrthoDB" id="2507774at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="315"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="398"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1108339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="455" mass="51571" checksum="2C494CEE224BD692" modified="2019-11-13" version="1">MDPHLQNAHSRIRVFLADLNLVLGSTSYATPYEPAPHFNQIGFPQNFALRGPHQQLSTKRPTHRSRAWKKNHARMDYQRSEQVLHALPILPVKQTIGSTPKEYKVRFPHSRWPAHISNHRERYRPRKDSEIASQIRTVHQHNSLHYPTDVAVDQQCFEESLHQHFSVDYLDREEEMVANYPSDTVDNMIEDQGINFCSDNSIVETCELDELQLDEIEWDDRSDLDINAAPDYFQPQLLTSQTIPKDSVDHNFPETNSTQQSEPDFDLKKYSAKDVDNWASTLSADEFEHLRVTGPDARTESFRQYVITNLDQSTQTLNQQSSSNHEHLEPPVSMPNGCHDNSKYHHHQPDDISSNNLEQQQLASPDDESRSSFCNHSDDCNPHLEACDNGGFEDDDFDNGSFDGGGFDDGGFDNGGFDDGGFDDGGFDDGGFDDGGFEDGGFDGGFDDGYGSPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5Y8</accession>
    <name>A0A5B0Q5Y8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018686</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5Y8"/>
    <dbReference type="OrthoDB" id="10316917at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="189" mass="21562" checksum="AE9EEB8612D5C658" modified="2019-11-13" version="1">MLSLIYADLPPNDKKMSRQQIDSQVSLNQPMQIRMTYARLVMIHYYKNKQDNDSQWTAIDDRLRVLRASSKEFQQVTIPSSPNFSHAQLVMDKDDQLFSHKRHINQITKDECVVPTLDDVQAAMAAGMVELADGKLLYTYYWSPNKSLSTTSFCVLVTGIPTRACRRQAPVYTLLTSQQKLADNQDTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6Q9</accession>
    <name>A0A5B0Q6Q9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4</fullName>
            <ecNumber evidence="6">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Carbon catabolite repressor protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Cytoplasmic deadenylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Glucose-repressible alcohol dehydrogenase transcriptional effector</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">CCR4_2</name>
        <name evidence="25" type="ORF">PGT21_029152</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6Q9"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09097">
        <property type="entry name" value="Deadenylase_CCR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000037">
        <property type="entry name" value="Glucose-repressible alcohol dehydrogenase transcriptional effector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF100">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="24">
        <location>
            <begin position="485"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="214"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="269"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="79"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="158"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAA1108926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="823" mass="92705" checksum="965D1969E6D55307" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7K0</accession>
    <name>A0A5B0Q7K0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-galactosidase</fullName>
            <ecNumber evidence="3">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_029627</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q7K0"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000302">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.20.10">
        <property type="entry name" value="Beta-galactosidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.390.10">
        <property type="entry name" value="Beta-galactosidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018954">
        <property type="entry name" value="Betagal_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037110">
        <property type="entry name" value="Betagal_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025972">
        <property type="entry name" value="BetaGal_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036833">
        <property type="entry name" value="BetaGal_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025300">
        <property type="entry name" value="BetaGal_jelly_roll_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13364">
        <property type="entry name" value="BetaGal_ABD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10435">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13363">
        <property type="entry name" value="BetaGal_dom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01029">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117100">
        <property type="entry name" value="Beta-galactosidase LacA, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Beta-galactosidase" evidence="9">
        <location>
            <begin position="69"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1108944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="757" mass="85336" checksum="2627EBC4E834A98C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC49</accession>
    <name>A0A5B0QC49_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_022493</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QC49"/>
    <dbReference type="OrthoDB" id="2500308at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="539"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="429"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="478"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="725"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="796"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="888"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1090"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="987"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="902"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="925"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1110473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1248" mass="137835" checksum="360DC75CCBF89E7E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QE03</accession>
    <name>A0A5B0QE03_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Peroxisome assembly protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 16">Peroxin-12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PEX12_1</name>
        <name evidence="20" type="ORF">PGT21_000823</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the PEX2-PEX10-PEX12 retrotranslocation channel, composed of PEX2, PEX10 and PEX12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QE03"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16451">
        <property type="entry name" value="mRING_PEX12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001163">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038074">
        <property type="entry name" value="Peroxisome_assembly_p12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0436">Ligase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pex N-terminal" evidence="19">
        <location>
            <begin position="19"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger C3HC4 RING-type" evidence="18">
        <location>
            <begin position="342"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1111466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="393" mass="45289" checksum="79683F070D322564" modified="2019-11-13" version="1">MDPNEEELYRPTFFELVAQDQLRELIGPVIRYVTSVFAQRYPRYLIKLLNHHDEVFAVMMYFIERYYLKNWCGSFAENFYGLSRRRHRIAKPVSLPDSTVGPQADPGRLAPHNVRLSLIFLVAIPYLRAKAAGLHEALGGGPVNDDLLDDEPERPSPLSNTDDSRMERIKSTTLNCFKSSYPYFIAMTEFSHLIFGLRYLFGKSPYWRASQAYTGIEIRRTSAHDQGRMQHKLDSQKISPFARDLATGHRPNWRLILRRFQSMISHRFFESLKVLLPGSIFFFKFLEWWYSSSNTSRYRPSFSNSNGTQFPAIQPPVPLKPQVKGILGDGTALTKPIPKGHCPICRTKLSNPTAVPSGWVYCYKCIHPYVVEYQQCPVTHFPTNLTNLRKIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHS1</accession>
    <name>A0A5B0QHS1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_002612</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
    <sequence length="1405" mass="155696" checksum="38BCEFFE5B758F9A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIC5</accession>
    <name>A0A5B0QIC5_PUCGR</name>
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        </lineage>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Pretorius Z.A."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">21-0</strain>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="1">Uncharacterized protein</fullName>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
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                <person name="Silverstein K.A.T."/>
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                <person name="Pretorius Z.A."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="142" mass="15876" checksum="119B05DFED70F76C" modified="2019-11-13" version="1">MSSQAKPGEAYKRRKRKLSEDHNLYLRASRAIHEITILDKDASHLGARSERVSASANGQSLFASESELFGGVLLRTMVSGGLGWRAAWLRPYEEGNLVRLGSPGFSHAAPQSPIQIFQNDDVAYTRERRNQRIMSPRAHSEL</sequence>
</entry>
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    <accession>A0A5B0QMH1</accession>
    <name>A0A5B0QMH1_PUCGR</name>
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        <recommendedName>
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        </recommendedName>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        </lineage>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
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                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="protein sequence ID" value="KAA1114456.1"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
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        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43948:SF23">
        <property type="entry name" value="DNAJ DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G15460)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JDOMAIN"/>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="517"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="539" mass="58892" checksum="EEE6289E4236067D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMV5</accession>
    <name>A0A5B0QMV5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMV5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022714184" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="7595" checksum="8C1F257E97B28C02" modified="2019-11-13" version="1">MSGSALCMSQLFAARSRSVLCQDVCACIFTSPTPDPQRPKSTGAVVPKRCPKTKTLRTVSPRVSKGSVSLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNJ1</accession>
    <name>A0A5B0QNJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNJ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="154" mass="17402" checksum="3D7671FDF6FBA898" modified="2019-11-13" version="1">MARSFSPEDDLKPELWTELGAEIVLIASMSTRRLPTTEEESLDNHQGTTRTSQTEDTSSKTNHLQSPKTSEKRERELETNPEELNKKIKPDTSLVGWKDFLPVDMYLVDWKEILPAVEVQVPRRLEGNPSSLGYPLGYPDTHQYFAEKGPSASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPU5</accession>
    <name>A0A5B0QPU5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine-protein kinase M1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MEC1_6</name>
        <name evidence="2" type="ORF">PGT21_034561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPU5"/>
    <dbReference type="OrthoDB" id="2511813at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022753334" evidence="1">
        <location>
            <begin position="23"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="219" mass="24541" checksum="6AB47409252BAD27" modified="2019-11-13" version="1">MYLAGWKTLAISIVHCFELTRATDLELVDQGCVELNKSLGHRICTILLRQMCRRCAYLLGVIKTEMSFFSRRIGLSIYASLDRYLPSLSQFMLIDLKFDSYGSALFADLLGISLEIFLTSTARSTLPLLVLNSNTTTIEAIAKAKEETVPGLLVYQAADILTELCSRNTTKELKKGAEIFYRLLNSGCENSSDPNQISIQIVKLMEMSAGLIYYRLIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QU79</accession>
    <name>A0A5B0QU79_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QU79"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="85" mass="9486" checksum="59DC227C4C8513E8" modified="2019-11-13" version="1">MALLPPTEDGHPNSIKYRRLLNYSSLPVARNSCLVKDFLRIPSGLIRRQTFHPPPTTHCNKPLYSPSPSSCPTRGPRFTSKAQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QUI4</accession>
    <name>A0A5B0QUI4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QUI4"/>
    <dbReference type="OrthoDB" id="2508522at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="127" mass="14343" checksum="1C8BE9A9193644B6" modified="2019-11-13" version="1">MAESLGPEAIQALELIDKHRCAMKNDLYRQVIQRESEMALFVDTKSKLATLREIVQRDLGYPVTVKHPRLAYLRKNAVGAPMKNLGTPGTPHPDAQGQLPRPESRVFLIFIGLSYAALFYLLLSYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QUT2</accession>
    <name>A0A5B0QUT2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TFIIH/NER complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TFB3_5</name>
        <name evidence="2" type="synonym">TFB3_3</name>
        <name evidence="2" type="ORF">PGT21_026037</name>
        <name evidence="3" type="ORF">PGTUg99_034767</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QUT2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="131" mass="14402" checksum="7F9BACBB7A73D96A" modified="2019-11-13" version="1">MFEDDYVITANCNAHVVEEKNHQQLGCHQPKFDHQKTRVRHNRTNITPSKQTTTQTMSGRKPAGAQSSKLGKKVPPRPTTHLAPTNQIGSASNPLANSNSFSSSLHNKAVTGPSNLQRDQAGRIVEFFVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX98</accession>
    <name>A0A5B0QX98_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10349101at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1331" mass="150920" checksum="78F566369875CB51" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXK6</accession>
    <name>A0A5B0QXK6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 15">Aurora kinase</fullName>
            <ecNumber evidence="1 15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">IPL1_2</name>
        <name evidence="19" type="synonym">IPL1_1</name>
        <name evidence="18" type="ORF">PGT21_026894</name>
        <name evidence="19" type="ORF">PGTUg99_001649</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
            <strain evidence="19 21">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1 15"/>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000048">
        <property type="protein sequence ID" value="KAA1134609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXK6"/>
    <dbReference type="OMA" id="ESRFPEW"/>
    <dbReference type="OrthoDB" id="377346at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044779">
        <property type="term" value="P:meiotic spindle checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902115">
        <property type="term" value="P:regulation of organelle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072479">
        <property type="term" value="P:response to mitotic cell cycle spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="131"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="259"/>
            <end position="260"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="12">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1117900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1134609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="404" mass="45405" checksum="1F68FDB49C6E6979" modified="2019-11-13" version="1">MTSRVLQRGSQIDQIENSLSNVRLDESSKPKKKADTQNSNDQSVPGSFSAPKSFNQSKNKPYALAPPPSAPPISNPASLANMGIGQYDGGFERDEASGRGSWDPGHKPANDTLDFEIPSPLPKTQWSLNSFDIGRALGKGKFGRVYMVRTKAPPQFIIALKCLYKRELVECKVEKQLRREIEIQSNLRHPNILRLYGYFHDEKRIFLMLEYAGKGELYKQLHRHGKFSEKRSAKYIAQMADALHYLHRKHVIHRDIKPENLLLGVEGELKIGDFGWSVHAPGNRRKTLCGTLDYLPPEMVEGKDHNEKVDLWALGVLTYEFLVGVPPFEDLSGHNATYKKISKVDFSIPPTVSSEASDLIRKLLRHEPQDRLPLPQVANHPWILAHVRTRNGTSGGGSNTTKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXV3</accession>
    <name>A0A5B0QXV3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rRNA-processing protein FYV7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXV3"/>
    <dbReference type="OrthoDB" id="2503175at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41805">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41805:SF1">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN FYV7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="24742" checksum="254EA221EB40A158" modified="2019-11-13" version="1">MTIPPKKPTNQQDPSWKRSKGFRIGKNKPGVSQSVYLGKAEKIKKTLIHKAKLKKQRAKELAKAGYDTTTSGPAPESPTDNHHQEDQPAGRTDSRTTQPGPSDSRRPKKSHQERPKDSSHPSTTKSQNSSNQQDRHHPKSSARAQQNHHPSSSELKASLKESTTKTRDPQQSNHHLKTPNRPARAKIQHANGQPKLSHRVSKILSKLEHEKEEGQSRLSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYR4</accession>
    <name>A0A5B0QYR4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_003935</name>
        <name evidence="13" type="ORF">PGTUg99_004805</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000250">
        <property type="protein sequence ID" value="KAA1118436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYR4"/>
    <dbReference type="OrthoDB" id="2125469at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10951">
        <property type="entry name" value="CE4_ClCDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471:SF2">
        <property type="entry name" value="CHITIN DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474523" description="NodB homology domain-containing protein" evidence="10">
        <location>
            <begin position="18"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="11">
        <location>
            <begin position="77"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1085341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1118436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="300" mass="33711" checksum="5E70B5AF6B0874F7" modified="2019-11-13" version="1">MNLWILLSALILLPAAAFTNSVVTLPAHNGNQPENQQQQRQADDNHVPATNHSRTVGKHGAHAQSAFKVYNTCKLSKSFSLTFDDGPTEYSAKLDKTLEKANLRGTFFINGNNFGCIYDHSEVLVERFKKGHLIGSHTWSHVHLTEGTYEQINHQIELIERAMIKILGVKPLYFRPPYGEYNDDVIKVLKKRGYKGLILWSEDSQDSLTSPPSPHEMIKTYQTYSDKTIVLSHETHPFMIDEVVPGVIPKLKSKGFKLLPVADCLELGSTPNDWYEVVKMPGSKDESWTCDGTPAPGMFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZW8</accession>
    <name>A0A5B0QZW8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ACB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_004884</name>
        <name evidence="5" type="ORF">PGT21_031307</name>
        <name evidence="4" type="ORF">PGTUg99_011536</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="4 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000444">
        <property type="protein sequence ID" value="KAA1079066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZW8"/>
    <dbReference type="OrthoDB" id="346910at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000010">
        <property type="entry name" value="Acyl-CoA-binding domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF62">
        <property type="entry name" value="ACYL-COA BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="3">
        <location>
            <begin position="4"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1079066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1094845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1118760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="107" mass="12214" checksum="73CD77FD28C7C78A" modified="2019-11-13" version="1">MSEIDKKFDRATELVKSLPSDGDDKPSQEEQLEFYALFKQATVGDVNSSRPGMMDFTGKYKWDAWKAKEGMKSEEAKTKYVELLKSKLEKSSNQEQAKKILEQLEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R055</accession>
    <name>A0A5B0R055_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_010207</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R055"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF9">
        <property type="entry name" value="AQUAPORIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="177"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1118912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="45381" checksum="61883F2D675A9083" modified="2019-11-13" version="1">MQAENEAAETIDSATWNQPQPDPAAEPETRGTSLDNDRGRGNYVSSNMTHEATIGRPAHRLMRLQTFSLSELNPQKSPERDRPLYSLGRPFPEPTALRRRSPTKLIEDIAAQMNLQTGSLKNFPISTNRPPESRAELAAGSVTQTGMHILRLGDLSEDQYIVATPELLRSFLEETRNRDSRRVRTSFPVDDHRNSRGTQASQRRHRADEYQDPVARPEFIGRPDLSESTAVEQNEKDNVPVSPKSYRSSNVSFVRGAGGLPPVTIARDHTGNPTYSSRYRRFKSLIREPAAEALASFLLAVFSGGASTQVYLTANSAISSSPKGNYATQCVGSAIAVMIGVYVAGGISGGHLNPTVTISLAIFRDLCAILNFSTLAQAGKANPFFVTQSPWQKVIPYCSAQVFGGLMGSLTIQLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2X9</accession>
    <name>A0A5B0R2X9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2X9"/>
    <dbReference type="OrthoDB" id="10507944at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="54" mass="6101" checksum="0C78625A3C0D6848" modified="2019-11-13" version="1">MRSCASFRSFCFNSKIVYGALGALSKSRPQTSTINPEEKDRVFLSSINDNRRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R8T0</accession>
    <name>A0A5B0R8T0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carbohydrate-binding module family 19 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031590</name>
        <name evidence="3" type="ORF">PGT21_033437</name>
        <name evidence="4" type="ORF">PGTUg99_013651</name>
        <name evidence="5" type="ORF">PGTUg99_028912</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000239">
        <property type="protein sequence ID" value="KAA1121254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R8T0"/>
    <dbReference type="OrthoDB" id="2362516at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1120631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1121254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="32222" checksum="F47FACA6126E8FF6" modified="2019-11-13" version="1">MYHPRIPLVVLILSSQTHSRFIEPGVVPINPIHLQRRGIDQNVLLENGQEAQKLNAKFSDMNANSPCKTGDSACIAGGFSQCVGGKFVGGPCAQGSKCFAMPLLLKKGTSLGCDTEEDATSRIKNTGATGGLTGTGGDSSKPSTSNSTQANPTGDMNGTKSDSNEKDGDSTGSTGNAAADTNKNSTSMDSSAGSSRPSDASDSGTDEKKKSNPPMDSSSGAADSSDPSASGTDKNDAKNSTDSSDPKSDSGKASEASKNSTSPSDGKEPDPKESSSNSTDVKKNEAPVKGADGEDVEDLSFAKDKSEEKDCADES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RI04</accession>
    <name>A0A5B0RI04_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012172</name>
        <name evidence="3" type="ORF">PGTUg99_035227</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RI04"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="137" mass="14820" checksum="E40C420E9259A9AC" modified="2019-11-13" version="1">MQSLLSPGVLPSTGDEVLRQFVLDSDEEIEDSPSSKRDNSTNIEMTVFGDDDIIEMRVGPRHFTNGNRRAVAVKREALDKNRLNGSPRSVGERSSERGPTPANASSFTFGGDHGRTGVSSHQTTESLRTTSGVKSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RII2</accession>
    <name>A0A5B0RII2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007326</name>
        <name evidence="3" type="ORF">PGTUg99_037076</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RII2"/>
    <dbReference type="OrthoDB" id="10281882at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="124" mass="13344" checksum="5D9ED1949CD702F9" modified="2019-11-13" version="1">MRAPDLGAVGAMQMQGWATPGHSHTQRPGKSCVDLCRVPLSGGYMAVPKPISHESPLTETQGRDSRRRQSACLVRGGEKPSLGDWPDSIECTDGEDLSRQLPPACNFVSFSVILEDGVSLYVCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJD2</accession>
    <name>A0A5B0RJD2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017759</name>
        <name evidence="3" type="ORF">PGTUg99_021613</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000174">
        <property type="protein sequence ID" value="KAA1125926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJD2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="58" mass="6427" checksum="5622E1E142A00563" modified="2019-11-13" version="1">MDPESGTTGPSVCKERNRQHQPALPVLLRGTGLFAICDKLNRSEKNSTGTTGWTRRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RNQ2</accession>
    <name>A0A5B0RNQ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013805</name>
        <name evidence="4" type="ORF">PGTUg99_021537</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RNQ2"/>
    <dbReference type="OrthoDB" id="2507194at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366656" evidence="2">
        <location>
            <begin position="19"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1126798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="486" mass="54912" checksum="4195E3AE3384BE50" modified="2019-11-13" version="1">MAPSKLAFLVLFVSIMESAHEIGAIPHPSFKDARFVDSAHVPVQMPTVQMHDVEKPLAERVSSSLQGPLEREQFHDRQIHESIPSQDDPASLVDSWFAPSPKPSSTMSDVEPVEEAVLDAFRYAGFIINDSQVKVIKSTPRSNNDEDLIPSRREHTESSLRWNPTLPRPLKRARFSDESVGSIRQPRWSAENNELPTESNIRWTKSSSIPSHHDHENLPLEKQAGVSSSSGKVESDSTTSRSMGQDPVESSTVQKMDLPVKQPLKKQDWMAGQISFFLSEYSKNLHTIYLRLGKLYDSSNTVRTISKKRMIGVPVVMTSIQMGESPLTLLNWIVKTDGNHCPVKELISKFKHLANFITCYHSELLKALGIDVSEVGPLNEALLKWVLNEIFNPSTGFPIIGMITDQKLVQGLGKEESPFRDLDAYLSIYFSRPEADQDSRKDACVLINYWYRSQQPQIHQQFSGNDKDHFMHLLKKIHMDFSVPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RW86</accession>
    <name>A0A5B0RW86_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_008930</name>
        <name evidence="9" type="ORF">PGTUg99_003551</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000136">
        <property type="protein sequence ID" value="KAA1129439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RW86"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF74">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="80"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1111700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1129439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="551" mass="60155" checksum="A58FA24034C6EF8D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SAJ2</accession>
    <name>A0A5B0SAJ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027074</name>
        <name evidence="3" type="ORF">PGTUg99_002712</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000055">
        <property type="protein sequence ID" value="KAA1134495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SAJ2"/>
    <dbReference type="OrthoDB" id="10278045at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="357" mass="40197" checksum="85F26865CFDC818B" modified="2019-11-13" version="1">MAEKTDVHPLKGIDYSEKDIGVLVRQLCKKLNQQHSQCLTEVVDFDLQAVFLMQEIGPHHRIRSIVKMVLKQFDINTQPPKTPKKRSPSSAPPQNMLQQQLLDAAKQVQKTIDTFDNNTSSRKPFCLQFPSYKHVLQNAISMQDKCQKLPTNRMVVLLDEAGICVGVVVPPFPKTPGKHHPEADDCACQALNKTVLTSSWNTNEDEVQYSSTPLDPHPRSPFPLGKSNKGESRTPKGGGYKDVDTKSKGMNDLKIPILKNQINTAKLQGYSNSWKNVPFTPSLPNSLRKSQNIQDTQARVHLRNEIKFYSKLSLWINKSFLPASTKITEQGVQYLMSSDSDLLRKNLAKENNQIIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LHY8</accession>
    <name>A0A5B0LHY8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-1,4-glucan branching enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GLC3_2</name>
        <name evidence="3" type="ORF">PGT21_000287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000200">
        <property type="protein sequence ID" value="KAA1063979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LHY8"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 13 N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1063979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="62" mass="6986" checksum="417B25E61E7D37E5" modified="2019-11-13" version="1">MKKDQFGVWECRVPPKGSQPGIAHGSKVKISMIAQSGERIERLPAWIRFMIYSIYGSFAIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJ20</accession>
    <name>A0A5B0LJ20_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SURF4-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_016842</name>
        <name evidence="9" type="ORF">PGTUg99_032713</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000506">
        <property type="protein sequence ID" value="KAA1068312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJ20"/>
    <dbReference type="OrthoDB" id="7859621at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002995">
        <property type="entry name" value="Surf4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02077">
        <property type="entry name" value="SURF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01339">
        <property type="entry name" value="SURF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1064857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1068312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="51624" checksum="C6256514ACDE5AE6" modified="2019-11-13" version="1">MASPQINVTPASPYNGQQQQQQAPGSTMRRQATGSPNPSGGGGAGGGLPGSPGGGGGFSYPSAPYTQTGSPSPSAYTPIGTTNSYISHPNIAHQRQSNHPNLANDSNPNSFLAKIQLWSASIEDFIELYSHPIKPYLPGLGRFLIVVTFYEDALRIGTQWEDQLYFLHKHRHFPWGLSHGFLISNVVVMVVCSTLIIAKKYPLSSVLGLLGVVISQGVGYGLISDLNFFLRNLSVIGGLLMCLSDSLSKRNNPKANAIFAGLPNTLGTETERRTYLQLAGRILLVLLFVGFLVSGEFTPARAAVSLIGLVASAMVAVGFKAKWSAMFLLLVLSVLNIVVNNWWSVHSAHPHRDFLKYDFFQTLSIVGGLLLLVNMGPGGISVDEKKSQPFSFWIQLILITQLTPFIQFFIQRSFDHQLYYNKHSKKMFFFSLSFSFIIINNISLPLFSSLFFRFSVIQCDFLFFLYSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMB5</accession>
    <name>A0A5B0LMB5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMB5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="244" mass="28950" checksum="173591913E489B21" modified="2019-11-13" version="1">MFEIEFIIENMKFHASHCGELQRNHMFKSFFNSFSSGKYIKKLQVGGGSNPSRLFKGISNQIEPVETNDYNDFKNEIEKIMEIQKLILEQIEYDSHEVKLNTAIFEFLNFVEGEMFPGITRKILKNSPQDSEKFQDMLRLQILGYQFSNLRELACNYHSFTHSKKAHVRSKAIFDRRICLKYKDRMLEIWDEFLDKYKEVSAKNDNYLDSTSMRIVKFYINNGNLIIDSLHKQIMEDKQSETFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMD1</accession>
    <name>A0A5B0LMD1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMD1"/>
    <dbReference type="OrthoDB" id="10350904at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023028929" evidence="1">
        <location>
            <begin position="24"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="143" mass="15815" checksum="7F4CDC0C16827551" modified="2019-11-13" version="1">MFQTKALILRALYVACGITLVVPRHSLVPRGTIAMTPCYSGLNPDYVRGGDWLECQPYPGQPSVSCHSRACNAGDSTESTSSFSSMTWEDCERIVTSQVTGQKGLRVTKTSNFNVNYSDGPEAHICMYLSFNCLPKDFQEIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMS4</accession>
    <name>A0A5B0LMS4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAM192A/Fyv6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_013623</name>
        <name evidence="6" type="ORF">PGTUg99_009424</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMS4"/>
    <dbReference type="OrthoDB" id="75720at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039845">
        <property type="entry name" value="FAM192A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019331">
        <property type="entry name" value="FAM192A/Fyv6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13495">
        <property type="entry name" value="NEFA-INTERACTING NUCLEAR PROTEIN NIP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13495:SF0">
        <property type="entry name" value="PSME3-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10187">
        <property type="entry name" value="FAM192A_Fyv6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM192A/Fyv6 N-terminal" evidence="5">
        <location>
            <begin position="18"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="159"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="63"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="214"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1065366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1090782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="259" mass="28562" checksum="03D9778D2E9720A0" modified="2019-11-13" version="1">MADEPINLVLSSQVASRFVSSSDLETARQKREEEWKATYQRLGQEAPPMPNEEYDGRSLYEKLKETKDKKQEAFEEKLKFKNQFRALDDEEVAFLDDAAEEKRAIEMAKQEEIQKEMKRFKEAIASRTAETPVIGSVGTTTAAEIIDKGSAPTSVSISHTVATGSTNQKTTTMIPKKSRKAKDFQRAFLAGAIRPKSTTDQQGASATKKRKSLDTSTPAPAASNQPIASSRPSNSPDLANPETSQPTQDNPPDPKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMV8</accession>
    <name>A0A5B0LMV8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMV8"/>
    <dbReference type="OrthoDB" id="10341820at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="207" mass="22338" checksum="E80A8D938D1EE808" modified="2019-11-13" version="1">MAPVSEDTPPAPMQTGQARAICTPAASTKSTEQDLDPHRESANNPHTPSPTLCKNDFFSLPNPIGTPWTTCPTFVCFPRWNRESIEQNFAQSNPLPRPAELPPAPGLNTAPPSTLFTSAPAPSLRPHPQNPVIHEPTSRPSKYRFATVQLKGMDDDVVGKRAHLKSRGSDEDADGSTNYSDDELDEPPAQQPVPVGLYHPSGATKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LP92</accession>
    <name>A0A5B0LP92_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_005677</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LP92"/>
    <dbReference type="OrthoDB" id="2503717at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="5">
        <location>
            <begin position="47"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1065630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="172" mass="19607" checksum="C1C7007556C3AFCF" modified="2019-11-13" version="1">MAGKNQIKDTNDIKVVQPNNTESKPKRTAASKSNLAAVSATSHRSWVWSHFKLVSNNDTDTKSMERQVRVKTKTEDKLCGRALQRDPTGSTKSMSEHLRRLHKIYPPNQEKTNQLALPNLFKRQCVEHRAISYLVAKADLPYSIVERKSFQYLLELLNPATLNMDFGQKKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LU73</accession>
    <name>A0A5B0LU73_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004306</name>
        <name evidence="4" type="ORF">PGTUg99_021495</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LU73"/>
    <dbReference type="OrthoDB" id="2512225at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366051" description="Hydrophobin" evidence="2">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="12920" checksum="2B19E8A18F40FCBA" modified="2019-11-13" version="1">MWAIKFFSILILIVLQGQAVYTHPRKSSTSQRSKDSTSSDNKPFSCPGADRAICVFLGTTGTSVQLARKNGLQYSCDNVPRKCCSDALKFDEEKAKYPGVTPVLQGEVTNLNICKEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUR9</accession>
    <name>A0A5B0LUR9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009479</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUR9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="159" mass="18135" checksum="3602DE302613EDA3" modified="2019-11-13" version="1">MQCSNTYNQLTVLPPTHWDSTKLQLIVVPGTPGSNLYNSGYCSQYQAGHLFQEPWSTQAQYPYPHNSLTKSVHLQMPKGQTVYFQTPDFPGTPSSTIHYGGYPIQYQPGHLYQEPWSIQAQYPYPHISPTKSVSPTNTKEKSKLIQYPQRYHKGWGISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LV33</accession>
    <name>A0A5B0LV33_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LV33"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="82" mass="9155" checksum="97FE74876F0BA479" modified="2019-11-13" version="1">MAPFINVVQSVSGTILREQGIFVHTSRMRASSYKKPFLCWSPLRPVRYPPARSTLSPDSGQPSGQDQQDDEGSSIYRHALGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M166</accession>
    <name>A0A5B0M166_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033419</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M166"/>
    <dbReference type="OrthoDB" id="10290465at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023019771" evidence="2">
        <location>
            <begin position="23"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="363" mass="41143" checksum="BA89811112AE082D" modified="2019-11-13" version="1">MHLLVRFSCFCPVLCCFARVLAKFKFDLNLPADVEEISGEEQRLVADHHDPFPPSPPDSSTPSSSVTSPQAMGGSKRKCGPEETHRLSNLSPSRLPRQHDTQAPSTSNKRQFTRLQKEAAPSRSNSPSTQPADNVIESGVGELRNKKANPNLVRPDILYLSGIPNHSRCLLGDVSKRWFSLFESIVNSKEFKSAPRPANIVHPDLPFAMVQPADGREGRVLRVMRYEDAAKRKLALPRPQNCVSFGRRLIVYLNHLHLEVMERLGLPESTQILLHDDFLKWVHGELFHPSDHVLPVLGTIDKPYQSWEEILELNKIGKNQEEIIKYFSGDGKHEEITKISVYLLETYQDHHENFYLPSSYLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2I7</accession>
    <name>A0A5B0M2I7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2I7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="350" mass="38275" checksum="AC5988C5D2CC1C38" modified="2019-11-13" version="1">MEAAQPAQGSSLLNKLGELEAKYLPRPPKWQISKNNNALCSELPESEKAIWYIDEKTIGASSENDSNFYDLYPGQAVAERKDKNPARREASIVEMGQFIRDPGAQATRNCNDLRLKREATKSKNESSDFRGLSQVEIACSLPRHERRPGSSVSSSWIHHDPTVTPPPQNNLPSRRDDSRVGTACMHGSGQTGWSALTSVSSQYDARSTRPHAQQGIDRWVNPACMKTGGMTAGSNPRLHEDGGMTVGHPNCWSTDGPTKARVNNLTTAGRIEKSRSATKAQPEFADTMAPRTPVNTTVLPPEAGRLKSIFRGDSRFQMGDIAMSTLSAPLTRGACDSDSQLRLRGRDGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2T9</accession>
    <name>A0A5B0M2T9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2T9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="80" mass="8627" checksum="DAB06397A694BE11" modified="2019-11-13" version="1">MEAARTKTIEPPFRVQPAADRRSFENPASCTPADDGSGYVMAGNWSQDLPGSVRFKPTPDPIAPPPHLKSGPSPRKLTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3A7</accession>
    <name>A0A5B0M3A7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3A7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="108" mass="12060" checksum="D99F46367A01BF14" modified="2019-11-13" version="1">MSSQGRHRASLRVPRTPYGHTQLNKATRTEVRGNRSHILSRNTELSLSLGLKFSRLRIPIPHCTRLPLGHRGSSLEVYCLNKATRTEGPGNPRGDLTSSAVIRSFLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MC82</accession>
    <name>A0A5B0MC82_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MC82"/>
    <dbReference type="OrthoDB" id="2512025at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="127" mass="14430" checksum="EFC09ED4E2E91C52" modified="2019-11-13" version="1">MAKLQMRPSHAYAGITTNGATSSIHVFLVARPSGATEELKALLCQAQDSQKVLQKLIPRKELEEFVKGWNPWTIKKELYPSVPKKNDGKKRSSSSKEVKQLYNDPNRWKKVMRGCNTLEAAYRHMAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCB7</accession>
    <name>A0A5B0MCB7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCB7"/>
    <dbReference type="OrthoDB" id="10351434at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="135" mass="15500" checksum="F96E394A9C0F29C3" modified="2019-11-13" version="1">METPQQDRVQEIANALSNLQINHQTEQQRSEFLTNEISSLKMDLNSIKLTLQDVTQRLLAFHHQLSNFQIPVPAPPPPPPPHPAFSAVSLMPHASFSGNPKEINQFIYFVKDRLIEAESCFLNEKRECSSPRFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCQ6</accession>
    <name>A0A5B0MCQ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">L-arabinitol 4-dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_002678</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-arabinitol + NAD(+) = L-xylulose + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16381"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17399"/>
            <dbReference type="ChEBI" id="CHEBI:18403"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.12" evidence="8"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCQ6"/>
    <dbReference type="OrthoDB" id="2148442at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050019">
        <property type="term" value="F:L-arabinitol 4-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF12">
        <property type="entry name" value="L-ARABINITOL 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="13">
        <location>
            <begin position="72"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="226"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1074361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="398" mass="43189" checksum="7628FEE0EF5D84C3" modified="2019-11-13" version="1">MCGSSSETDPVFQSINPSLYDPKHVLDSEEFEILTDPNQVKAYSDEDKNIACCYNDKQQLNMVKKPMPKAHPGQALVRVRATGICGSDIHFWKHSRVGEKMVVKHECGAGHESAGEVIALGEGVTDLQVGDRVAIEAGIPCSKPTCDMCRTGQYNACPEIIFCSTPPYHGLMTRYHAHPSCWLHKLPPNVSYEEGSLLEPLAVALAGIERAGIRLGDPVLICGAGPIGLVTLLACHAAGACPIAITDLSESRLSCAKRLVPSVSTFQVSLGEPETEVAQKIQSAMGCKPRVAMECTGFESSIATAIFSVKFGGKVFVIGVGKDKQTLPFMHMSENEIDLQFQFRYANQYPKAIRLVSTGLIDVKPLITHRFSLEKAIEAFNTAADPKSCSMKVQVVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ME27</accession>
    <name>A0A5B0ME27_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ME27"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="13129" checksum="69772EECA2F68F63" modified="2019-11-13" version="1">MQSITAVARGGLTFALIICLLYNVPKYNCLKECDLCAQQSRRMGSTSSTRNIMKFKGARGTNRCHTFQVVDTNQDNTNCAYELYIDQYHCDYCQSDIGCASCPEHGELKPMTISPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJC7</accession>
    <name>A0A5B0MJC7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029570</name>
        <name evidence="2" type="ORF">PGTUg99_026925</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJC7"/>
    <dbReference type="OrthoDB" id="2507853at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="365"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="478" mass="51554" checksum="80D116B0DADFC368" modified="2019-11-13" version="1">MTSVSTPHQHHHHHHHEYSRKSNNMPALTAYAHPPAHAGWTSLSSKPSALSHTSSSCSGSALAPAASPITQIPGRPRSTISRFERLTKPLSSSASRLKKTKSANNCQLPQQQQQQQQQPDIILATPRPQPAVRGILTKHSSLLNLSPRAPAVVESSPVAVVLEPTIVEPLCLLPSRAQMVTKKVRFSGNPQDGYGRMSSSGCSGQQEGCKALSQGQSQGCMEERCRRAPEPVIETIFLTYSRFAYDRSPIAVDRVFNKSLALPPRTDNNDDLGHGRWRADLLPPSLSESPISLEDCNRLNKHSFPAELAVDLDPTLLPRLSLSPVLQGPADPPPIFVAAHEGPESAASWQISSDLFGPTSHSEDGSTSSSSSQESPTTPAITELMSEPPTILYSDLSHLIRQPPLATAPTPSSTTPFSLSVQNLAHHALANLTLDNLPPPALDQRILTCLPLDNHPISGFGNWNRTQVFGSCDALDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJM8</accession>
    <name>A0A5B0MJM8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021719</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJM8"/>
    <dbReference type="OrthoDB" id="2518661at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="80" mass="9072" checksum="A045A82891CF1DA9" modified="2019-11-13" version="1">MNRTLTDTDKPLQHPTPNEVSKACQLVDGKFFLMNFVRVALVDPNDEESVIVVMEFTPWDQSTKTDKENLDFSSSILLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKS7</accession>
    <name>A0A5B0MKS7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Guanine nucleotide exchange protein for ADP-robosylation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SEC7_2</name>
        <name evidence="10" type="synonym">SEC7_1</name>
        <name evidence="9" type="ORF">PGT21_012837</name>
        <name evidence="10" type="ORF">PGTUg99_017258</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18431at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000002">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000014">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF375">
        <property type="entry name" value="LD29171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="8">
        <location>
            <begin position="914"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="430"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="832"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1305"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2030"/>
            <end position="2057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="146"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="474"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="497"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="834"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="860"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="889"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="904"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1313"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="2030"/>
            <end position="2047"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1076599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1126766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="2057" mass="227716" checksum="D0F5D24F429D867A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLF4</accession>
    <name>A0A5B0MLF4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLF4"/>
    <dbReference type="OrthoDB" id="10372055at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20785" checksum="7E5CEA774154AEA4" modified="2019-11-13" version="1">MADFLITGTSRRSRTQAAQIRNQARELEEHRAFLEEVSIAQQLGIVDANYAQQFAGPPHEDDEQERSQFEPLIGSYVGEDFENDVEQLPCAAHAAYHRARRYAEGRERMSQRWSELEEQVAAAFLICQKATKNWTSLPGTYTLPPNTCTCDPINVQERSMDLFDIICKPFFPLFLPGGPTNNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLK3</accession>
    <name>A0A5B0MLK3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLK3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022879197" evidence="1">
        <location>
            <begin position="20"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="167" mass="18993" checksum="AEF8B000A645C6A5" modified="2019-11-13" version="1">MFCEFLIFSLTILPATVLAPAPFKAAEIGGTTTSQRGIPLVRENRQLLQSSDQDFSVSGSFQALGTVIRAKGGDNKSSVPRFIEGWIPNNDLAYKKFLDQNLYFKENEDKLNAFDEDCKKLQILLEEISDQHKWATGFQGARSEDFRNDLESSNIIHEKMKYFYLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MS29</accession>
    <name>A0A5B0MS29_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MS29"/>
    <dbReference type="OrthoDB" id="2503202at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="163" mass="17313" checksum="EF87EEDCFC67702B" modified="2019-11-13" version="1">MSPNVHVDRVSEGADFHSVGSRGVDHPSSSITCSEGKPDKPCRAIRASTPSKYLRRTGLVPNRAHLKIDVGYKTSIISSSSSSTTSPSSTAITSSIGCKFSTTHRDTPTLPSLTESNLARLMKQLKLSAHAYSSDCSFDSTPHTAMEISYFSDGSSTHSGSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MS36</accession>
    <name>A0A5B0MS36_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MS36"/>
    <dbReference type="OrthoDB" id="2497716at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="706" mass="81315" checksum="6C2DB86DAAAE402A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MV84</accession>
    <name>A0A5B0MV84_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MV84"/>
    <dbReference type="OrthoDB" id="10320215at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="12202" checksum="1E499BEF890F8428" modified="2019-11-13" version="1">MYSSAPAVVPVYIVRVGTAATSNDKLYFGCGPKFPVGLCACKVNNQNNLYVSEPPSQRVGGHLEWPWCDSASCNTYCGNIALSDASYQGIQDPYNGLPKSYIASNSIYRSGHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXI9</accession>
    <name>A0A5B0MXI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014208</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXI9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="161" mass="18129" checksum="D8C6E4281D12A9E9" modified="2019-11-13" version="1">MKIENIAAVEVFGWWWWLPVDRRLLSGKAVQAAGSVVIFSVTKVIDKPRSTRSHPSQANAPRVLHNTYPCSRKLKYLHMYGWQFWGHRLGHKLWMHLGIKNFFLKIARFTADSATTPRPAGGSLPHPGDASLVRAITGEKFPMKPMGAFWTPITSLLLNGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYJ8</accession>
    <name>A0A5B0MYJ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034709</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYJ8"/>
    <dbReference type="OrthoDB" id="10314085at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1081366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="325" mass="36598" checksum="571623490C9BC17B" modified="2019-11-13" version="1">MVVEQLNDMATKGFVAYDSTIDQEVHVMTSALCFLADSPMHAEITNTHVPGNALNSCRYCVLSSDDLKSRQKLAYLAKFAQKNSHGSDCPNPLRTMEETKENSKKLWTETKETLNLDKLNAKSAKLAVRDQINLRFSKQVFNFQSEKIALLAAGEELPTRFEQDIPQKLVDMEEKEPKRMFNAYLELKGFDSVKDTPVEVLHVFLLGPNIESGLNAQHNCHRGTCQLTETLAVPVERRKTSNLALELTHNDNERFVVNLASLSSPALHREVSDVGFRTVDRLQWLNTMHDGLTVWLSTVKKKQEKAVKKNAVASTSRTRTDPGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYP4</accession>
    <name>A0A5B0MYP4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000129">
        <property type="protein sequence ID" value="KAA1081722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYP4"/>
    <dbReference type="OrthoDB" id="10568519at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="393" mass="42404" checksum="55E38991F9DA3D9D" modified="2019-11-13" version="1">MITGRINQVAVVHPRGASRARPPLGSARRVAGPSPRVKQATPSGALGYLGGYSNFSVRLFGRIPFVLVLIDCPLWAAVTEATEGRGRAVEEALFDNVTVRTPLVRVPAPRRCSVHVQLVSKLSTARTTRAATRLRSPPTTNPGGPPRSPLRAPQTTDTKRDARYYEHSGISDLSCDATTGNISARSTFDWLATPRVGTKNTDHESHGSRATYVHGRADVTPREGTYSRRAFSANHPKHQKKVRFLFAGRTFPHSHSACTVKMRTRPSPSVPRGHRAPQPGPLSSYRRFGNTNRSYSLLSDVHVRTPGPEASGYTSPPLGAAVGGRSPQPSRTTLSPSADALRTLRRARPASVPRRAARHGDASARRLETGAARCRADQAADRAGSSARLRPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZ60</accession>
    <name>A0A5B0MZ60_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Growth hormone-inducible transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_029527</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZ60"/>
    <dbReference type="OrthoDB" id="1277691at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF112">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1081109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="36267" checksum="B4B8E319ACBAC985" modified="2019-11-13" version="1">MSFLKPRLTITNRLLFQQQPLKLTQNNNLQSTRSIVSCLRPRPTNSSLIMRTNSAQRWFSCSNQTANQANRLSIFQQTKTNSNYRQIFIASAGLVGSLVLLDGFLNRDKRIEPLSAAQKDLLNSSFQHTGVGLAITSVLARYMFKNGWTMRMMMVNPLVFVGVGFLGTMGSMLGTRYTSPDNTAQKYGFWLTFTACQAAALSPMYFYAPAILSRAALYTVGVIGSLSYVGATAKNDRFLYIGGPLLAGVSVVALSSLAPLVLPATAARTLMVTESISLYGGLAVFSGFVLYDTQKILNHGLLVEQGRMKPDPVNESVGLIMDSINIFVRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3E6</accession>
    <name>A0A5B0N3E6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3E6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="8129" checksum="B0D88419CADC18AE" modified="2019-11-13" version="1">MPDGLSRRPPDPDEEDSPSFDEDEESMRPHPGFGTKAAFSVQLLGTTAKLGEEVLDQDVDVPKTLLAGWIDSVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4D9</accession>
    <name>A0A5B0N4D9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Monothiol glutaredoxin grx4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GRX4_1</name>
        <name evidence="10" type="synonym">GRX4_2</name>
        <name evidence="9" type="ORF">PGT21_017528</name>
        <name evidence="10" type="ORF">PGTUg99_022785</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Binds one iron-sulfur cluster per dimer. The iron-sulfur cluster is bound between subunits, and is complexed by a bound glutathione and a cysteine residue from each subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4D9"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000012">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000092">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF73">
        <property type="entry name" value="GLUTAREDOXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="34"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaredoxin" evidence="8">
        <location>
            <begin position="181"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="129"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00055846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1084101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1093336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="271" mass="30644" checksum="D96B79CDB9D49829" modified="2019-11-13" version="1">MNSSTNENAELVSSNYHKLESPTQLQSLLGADLKRLSILYFRAEWAEICRTADPFIRSLSQKWKEPLFLEIEAESLPEVAESFEVSSVPCFVILRGHQLLSRIIGAELTQLESDVEKFVKASQNQQSNGKYEVLSETTQKPKPPSSTPNVNKLQAGATSNVDETEEQLFSRCKDIMQQSKVVVFMKGDPRTPRCGFSQQTVKILQDLNIEFTTFDILTDEKVRQGMKKLNSWPTFPQIIIKGELVGGLDILKEMIQNNGKEKTELEELLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N608</accession>
    <name>A0A5B0N608_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034337</name>
        <name evidence="2" type="ORF">PGTUg99_015972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1072992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N608"/>
    <dbReference type="OrthoDB" id="194386at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF130">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EEF2KMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="406" mass="45103" checksum="4A2524864B8599BA" modified="2019-11-13" version="1">MDDYLNQSALLRSYAALTPNNPREIQTPPFPTSTWLSKHSNQLKLFNLLVSKQDHHQQGFNRKYIANGRQWKLMFWKRVILEIENGFNNISDPKLKEEEEIEEKILTYYISLQQSPPPHQNQTEGRGHEDEETLSPLDQTEIHTYYYGDLSDRDRWTRIDLHENKASISQGTTGLRSWGASVCLANYMILHPDLLADSTKVLELGSGTGLLGLLVHTLSPSTHVYLSDSNPAVLARLQLNINLNSLGDRDTLKLVELDWNADRSALPAPIDLDTSSIILGADVIYDPDLVPGLVRTIKLGLLSSHPSSSPASTAAAAAAAKSTEAEATRSYGLICGLVRDPSTWQKFIDTCQLEGLEVEEVEIDEAVEIGSVEGRGIVFPQALDRALLDLRMVRLSIRPSLSPPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8G8</accession>
    <name>A0A5B0N8G8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8G8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022728322" evidence="2">
        <location>
            <begin position="18"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="594" mass="64206" checksum="1177B4D35E994BC9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBC4</accession>
    <name>A0A5B0NBC4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YjbQ family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBC4"/>
    <dbReference type="OrthoDB" id="2495354at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11470" checksum="C7E823164171545F" modified="2019-11-13" version="1">MPFQTTFTLAKRPKGCHLVTEEVARNIKEGLKNCDIGVAHFFIQHTSAALSINENGPDDSVSHTKASLIGSSISIPVTKGDFHCSLDLHHRSGLIITSHFLSSVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND87</accession>
    <name>A0A5B0ND87_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND87"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="126" mass="13673" checksum="0BD1D1074A19F3E3" modified="2019-11-13" version="1">MPYPLGGMGEEVFSHRSSSLLSTIIIKTPRLPTGLQVRPSFTTSLSFSTPAGSRTTRSLFRMVDSTNEPGSTISISIPEEVEVEVTGKNGVVSGLEHGREGDTNRAEELHLSRYYGSMGLNYATHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDG0</accession>
    <name>A0A5B0NDG0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDG0"/>
    <dbReference type="OrthoDB" id="2505255at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="347" mass="39703" checksum="DCE994BDE8EF04A6" modified="2019-11-13" version="1">MMRMFSVFSRGTLQTCAPTSLLSNISKPPSPNILRSRGQRYLSQRRPKPSTFKNKSSKGVAIDPILEDDSQNLTKLSFDAPAKLRRQALEMYAQCKLSERDPTTRFAIVRQMRNLDLPPHQELWQGINATDELALYRWVDEAFGQLIYQKGLWRPSKDWGPEDDEAEAIQRVENLSLLRTAYEEQILNPLKERFGTSEVTIALSELRDDQKLYKEPAIIWQSVNRKTNEISLEWMSEYTADVVWPGLVSCQPSYQVARRYWEAEARLFSNLIGGVARGMGENGEMLPFDSRDNDDLGQTDSSLDENIHEFQSSILSSAQANKQAAEAVLIKKFLEERNMSSKTTVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIA8</accession>
    <name>A0A5B0NIA8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004339</name>
        <name evidence="2" type="ORF">PGTUg99_029388</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000407">
        <property type="protein sequence ID" value="KAA1088254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIA8"/>
    <dbReference type="OMA" id="SAWSEWI"/>
    <dbReference type="OrthoDB" id="2501481at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="581" mass="63507" checksum="3B30D48C34B83A77" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIS7</accession>
    <name>A0A5B0NIS7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIS7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="10851" checksum="CE06DE87D8022482" modified="2019-11-13" version="1">MGLAMAMEHRWTELLDHTHTVHELTSQKPRQMRAGHCNSQSLNLEAAHGPLIYLINLSSLKTCAIVDAIAVPTHSPITKTGATHNEHVPSHVSFNKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJB1</accession>
    <name>A0A5B0NJB1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_015851</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJB1"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022690217" description="RING-type domain-containing protein" evidence="7">
        <location>
            <begin position="17"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="92"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1089375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="214" mass="23630" checksum="96AE2AA5F77AF8C2" modified="2019-11-13" version="1">MVAWFLLIALVGISLQAEHSIELSQIQEVTQAADEVGESSLGQHHSSQLKANLPNPRPSDSNVIVEIEALKYPIDLNNTDGPIQESSQIEECPLCLEECTGPYKMLPCKHLIHTPCLEKWVATSLTCPYCRVPCPDEQPAPHTLVSGVPERNTPGLVQELTERARFQIQEHFIPDIEMQGLCRILFFLLAGLGGGVTGYFLASVLLSVILKYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NK00</accession>
    <name>A0A5B0NK00_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Leucine-rich repeat-containing protein 63</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_022780</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NK00"/>
    <dbReference type="OrthoDB" id="2510147at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF36">
        <property type="entry name" value="CASPASE FAMILY P20 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1089577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="58114" checksum="04FA37EE4FEA7F52" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLK9</accession>
    <name>A0A5B0NLK9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026709</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLK9"/>
    <dbReference type="OrthoDB" id="10404594at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022791224" evidence="2">
        <location>
            <begin position="27"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="291" mass="32357" checksum="28FF077EB3C013C4" modified="2019-11-13" version="1">MKSSASIRWPIVLAIHLSWLCFPSRGTRYSCLHKFWREPSDHRLGAAIKAHGILDNKRPVPPTLSQEALVAPQQQPRMSRQLDMESGSADSFAGADWPSLEPGSVSQGLQSPMNPVLLEKEYQANLADAFWRTSGSSASRGPKNKHVRIKPCLKGGTNRKYWPHEGFMNTEEFTGTNSEKSAKPVPAPPLPEAATFTPSNPGSGHGSTNTISEWNEDQEVSPLKSTSKDLLARPNAHEVAQCVGQKKRGRSRRVRFQDPKHDATLFYNPAYAEDTYDKTEEISWRSFFGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NN07</accession>
    <name>A0A5B0NN07_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_011078</name>
        <name evidence="3" type="ORF">PGTUg99_014819</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NN07"/>
    <dbReference type="OrthoDB" id="2515722at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033845280" evidence="2">
        <location>
            <begin position="28"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="403" mass="41195" checksum="A0E7AACD557A9EAF" modified="2019-11-13" version="1">MNTHIISRRSFFFVIFFFHIASNSVTTFRTGKSKTTGRLQLRSMAAKAHTPIGENRKLTKRTFGGKLDGPPISKDSGPPSPKGPGPPGPGSGGGDLSLSGAGLGGKNDTGVGSNKTPALGLGGDKDANLPGGKDKGLVPDDKDKGLPGPLSTPPFSPDSGPPSKPGPSAKSTLPPKSGSGFGSESSTSSLSEKIESKTIRVEGGKGKDCDKKKQHRPSEKSKDDSEINVSGPTVIKIEVSNNGCSTCCKTCSQEDKKADATDAPPKKKSPEIKPPPEISSGKGGDTPPPEITSGKGGDKPPSPSPSPPPDKSKTPSPPPSTPPPSITSPSSDQPNPTIKTPPPSPSPKPSPPPSPPPSPAPKPAPSPPPPGDKPGSGHRHIALPSLATISITLLLWSCLLQNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNI7</accession>
    <name>A0A5B0NNI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNI7"/>
    <dbReference type="OrthoDB" id="2506948at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053005">
        <property type="entry name" value="Nuclear_Pos-Cytoskel_Interact"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190:SF1">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="149" mass="17308" checksum="1D4D0C82424845CA" modified="2019-11-13" version="1">MMDFEVKCVGYSTDPKPEEEPDGESTLILEIARQEVQKPGDDRIGRRLLGRPKLGLVSIEFATEIGQSLLGEVRRLQSLLVVRDDELMEMKEEREGLWERNEHLMQQIKDYESKVDKFKEENGNLELNKQELQNKLTTTNDEKTRAETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NP49</accession>
    <name>A0A5B0NP49_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cis-Golgi t-SNARE syntaxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SED5_1</name>
        <name evidence="11" type="ORF">PGT21_021616</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NP49"/>
    <dbReference type="OrthoDB" id="421009at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15844">
        <property type="entry name" value="SNARE_syntaxin5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021538">
        <property type="entry name" value="Syntaxin-5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF3">
        <property type="entry name" value="SYNTAXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11416">
        <property type="entry name" value="Syntaxin-5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="255"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="17"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1091045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="346" mass="38648" checksum="1A5BF88F03EDD402" modified="2019-11-13" version="1">MDRTQEFKSCVASIRSRTASQRVPEHKQRLLDNSSPSRKNQRAPGARGEFARLAGMIGKDIQQTTVKLSQLAQLAKRKTLFDDRPVEISELTYIIKQDIAQLNQQIAQLQTFVKQNLSSNRGQKQPVDEHNNNVVMMLQSKLADTSMGFKDVLEIRTQNMKATRDRTEQFQFNTPGLATASQSDSPLYAAQQAGVASGVNRSLYDSKGKGKSTQDPPGYQQNEYLALDMGKNSNPGESSGPQGYMQMQLAQDNSDAYLQQRSTAIESIESTITELGSIFSQLATMVAQQGEQVQRIDQDTIDIESNIQSAQSELLKFYSSISGNRMLMFKVFGMIMIFFLLFVLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0F7</accession>
    <name>A0A5B0P0F7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0F7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="116" mass="12838" checksum="706EB403CF73750D" modified="2019-11-13" version="1">MIAHHENREKRAEGISCKLYIPEGAREAGGQMQSSNAIDPMAMECLSDGHGGSYVFRRKYDALITSTELETIKIGSQCFRQTLVIILTLVHQPRPSPSVSAMVCRVGHSTHRLDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1Q8</accession>
    <name>A0A5B0P1Q8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000077">
        <property type="protein sequence ID" value="KAA1095527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1Q8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="168" mass="18165" checksum="400A6904560B78F2" modified="2019-11-13" version="1">MHVPPSSHDHPLLKPTSTQLTQPRLKNSSSPKNRMHSPHQLPNLGPVHSTPGARQQEIFPIATAVMRSSRNIPDISNFASNTFGGFFGNSNNASPHSLGRPRKNSRQVTYPQDSQPFPSGDSRALMNGQPNAPLPHPTQLPNSAGGSSKSPSPSTNNNHIIKVPKLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8Q3</accession>
    <name>A0A5B0P8Q3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_013036</name>
        <name evidence="8" type="ORF">PGTUg99_037084</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8Q3"/>
    <dbReference type="OrthoDB" id="2499654at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070646">
        <property type="term" value="P:protein modification by small protein removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF7">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="6">
        <location>
            <begin position="12"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="168"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="5">
        <location>
            <begin position="334"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1096329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1132099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="70942" checksum="4A27BA9A66BBE0DE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAQ6</accession>
    <name>A0A5B0PAQ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAQ6"/>
    <dbReference type="OrthoDB" id="2515550at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022832229" evidence="2">
        <location>
            <begin position="24"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="831" mass="95766" checksum="5EEAC10FF5758ED7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEQ4</accession>
    <name>A0A5B0PEQ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEQ4"/>
    <dbReference type="OrthoDB" id="2386367at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022933672" evidence="1">
        <location>
            <begin position="21"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="572" mass="66638" checksum="D7013385A9B90596" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFI8</accession>
    <name>A0A5B0PFI8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">biotin synthase</fullName>
            <ecNumber evidence="4">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">BIO2_1</name>
        <name evidence="17" type="ORF">PGT21_032937</name>
        <name evidence="18" type="ORF">PGTUg99_005643</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="18 20">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000342">
        <property type="protein sequence ID" value="KAA1099158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFI8"/>
    <dbReference type="OrthoDB" id="2414104at2759"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000011">
        <property type="entry name" value="Biotin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="BIOTIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976:SF2">
        <property type="entry name" value="BIOTIN SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01060">
        <property type="entry name" value="BATS_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00272">
        <property type="entry name" value="biotin_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01278">
        <property type="entry name" value="biotin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="16">
        <location>
            <begin position="197"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="479"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="503"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1084613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1099158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="513" mass="56901" checksum="D3169FC37500BDE2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIA2</accession>
    <name>A0A5B0PIA2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIA2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="115" mass="13518" checksum="1CB9033EFB4B3817" modified="2019-11-13" version="1">MNSSYSANAHLKISRTFQSRSRAVVLYWVEQPQATYAISVCVFQQHISGGRLDRFLLALQAAYQEDIKFHEEKHRAQNRTFIRRHRLIETSYFGNTKRFTANKGSLDLTIQRYHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJT8</accession>
    <name>A0A5B0PJT8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJT8"/>
    <dbReference type="OrthoDB" id="2499072at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="325" mass="37488" checksum="9AD7AA075756C9A1" modified="2019-11-13" version="1">MWQHPWFNRISSSHQLPSHLPAQLKYQLIHLTNSTSSTQLHTISNNSDPSLPNSEPSQPNSKASQHNSKASQLNSQPSHISHNPNNNNNAPNSLIRPKLLYSNKNTRSSSIKLSTTKQNSKYKKRKASRSSPTKKNLSRNRSCVNQEQDDDEEEPEEEEEREEEDEPEEEQPDPEDQLDQEEEQEEEEQEEEESINLTLENFQTQLGSWSINKLCEVLGKSKKSCSNRVQPEVQDALLLLQQNYIKRKLMLSIIGNISETTTAKYLVISEQTPFPPKEKEVFSPPIFQFFSKITCSYDNENEDKDELAKITPTQEEEELYQPLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRC3</accession>
    <name>A0A5B0PRC3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRC3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="318" mass="35421" checksum="32528D002860A1B7" modified="2019-11-13" version="1">MFPVPPPTIQHSWLLMMPLDLSSFPIPDIFALYDRILKLIASSEKNLKHTFLDHHQQGRMSGNNTLPGFSSIGTVVSLGMAAAVARPLQQCPDSTLGIVLDLLSLGPTSQPTTVLIATWLYSERRRSEGAESAWMVWDLYLNHSKLISILTENDCGQHLSSDSILDRDLLFMLLAAMDFGRIVGSYRHSLSIDPSPLPVIVIVFNSQLLLPAPLDSVLKQRKFAIQFIAHLCLHIGDWNNMDIVLCQLGLTNAAVFYILDLQFTTHPKPTMSDNLLTVCQHLQSDDEETSSELSAEVTIHHLSFQPVNHRQPASKAHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRL2</accession>
    <name>A0A5B0PRL2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRL2"/>
    <dbReference type="OrthoDB" id="2511773at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="154" mass="17441" checksum="85AE1FA6BA607172" modified="2019-11-13" version="1">MFLLAIIQDPTTPGDDYSEDQPSKVTSDAPQDANPPSENETQNGRKKRRRSSYVDKKALNRYWPTHPAVVRPLNPGASPVRHPRALTLILHEGPPSRLRLGTPPRPHEQGRLGNPDENDSINQIPDDDDLNNRSRDDSQDEDEESRPRSRRRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PS79</accession>
    <name>A0A5B0PS79_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011413</name>
        <name evidence="2" type="ORF">PGTUg99_007437</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000143">
        <property type="protein sequence ID" value="KAA1128213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PS79"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1128213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="148" mass="16756" checksum="713BCFEA7B4B9F93" modified="2019-11-13" version="1">MFYPVIIGEESWKPAAGLSERKLPAGYNLWKNTPKLEIPSWSSGEPSFQHEGNHPTRAKFEWPSATEVAIVYNQSDHPMQFLLQHAETKAFVYCRSVQQGYGQRVIIPRHCRVGSLWLYIGPEVAEAAENERKPKSAITTFLSALNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT31</accession>
    <name>A0A5B0PT31_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006981</name>
        <name evidence="3" type="ORF">PGTUg99_013104</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000315">
        <property type="protein sequence ID" value="KAA1104927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT31"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="55" mass="6070" checksum="64B765AC74CF0FB6" modified="2019-11-13" version="1">MSAEIAVWNDESDKRSTLRPQKPTPRQPLAKLDKNLPLPMPSYGQKAAPAKAQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTA2</accession>
    <name>A0A5B0PTA2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_002763</name>
        <name evidence="3" type="ORF">PGTUg99_014346</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTA2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474229" evidence="2">
        <location>
            <begin position="29"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1103854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="460" mass="53577" checksum="72FB2132F0477704" modified="2019-11-13" version="1">MFSPYILSSLYLILLIFIEGSFSSPALGFDSISAKLNFRETPNFHGKPEGNYLQIHSSTSPHEDLSENNFNSDEESDYSSGYGSDESDDEYDSDHRSEDGEYHGGDKGHHDVKHEEYLILDSTGDTSSADNKLAHRTGRQNLSKKQQILFENLLQDYPGKEKLTTKDFLDIKENWKAHTRELINLEKDTRYLEEFFDLFSIRIDYKKGRGHPDEKKFFKDAELVGNLNKKMKILKKKKLVELGRINSEFQNLRNEKGEIYASSVKQQWDELISQPVNEIKNLMQDYKSQIQNIDGLFDHSDEANLVFAYLFQGLNFLYKNSFIDGETTRSFFQEDEFLRLALGYSAFFVKHHGITNEANSMSGVMINQWFWPPEFEFLRAFNEEDSLRINFEHLILAFARHGQGFEALQEEVKFLSNSFLDQRYTKQLIKHNGEKPSKMLDAKKYPEEWKILNILKINSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTU1</accession>
    <name>A0A5B0PTU1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Crossover junction endonuclease mus81</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">MUS81_2</name>
        <name evidence="1" type="ORF">PGT21_008624</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTU1"/>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="138" mass="15324" checksum="194BA80E950D0FF7" modified="2019-11-13" version="1">MESNQSNSDLVMRDYNCAALKNDLLMNGNPRLSESSEGSIGPWQWRAAAQISFGSGQSNPSGRRSSDDIQPEVRKLGSDFDICVVLDHREKISSPGRYLIRVLQRAPVVGNAIWIAVHRDDQHEFFSGLRADPISLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PWS1</accession>
    <name>A0A5B0PWS1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="3 14">2.4.1.258</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dol-P-Man-dependent alpha(1-3)-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ALG3_3</name>
        <name evidence="15" type="ORF">PGT21_003136</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 14">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyltransferase ALG3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="3 14"/>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PWS1"/>
    <dbReference type="OrthoDB" id="20028at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1105327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="425" mass="49282" checksum="14F07723C5221890" modified="2019-11-13" version="1">MSDHLKATLKYCRRILVSKEQYDLIAGLVLLSELVLNTAIIKYVRYTEIDWIAYMQQIRSYSLGERNYLKLKGDTGPLVYPAGFVHVYSVLYSLTQQGQDVKLAQYLFGAIYLITMGFVLAIYKRCESLPTYAIFSLILSKRMHSIYVLRLFNDGIAMMGLYVSIWCLVTRRWNWSALWFSLALSIKMNILLFFPALGFIYFQTVGVLKTINLMLIIVLVQAWLAIPFLLEFPREYLSKAFEFKRVFEYQWTVNWRMVPQSTFLSSGFASSLTLLHLSTLVWFAWTRWSRASRGDSLISLLVRGLRNWNRPAAARPDQLTPDYIVKVCFTSNLIGIICARTLHYQFYSWYAHQIVFLAWQTPYPIFLRILLMLSIEYSWNVFPSTSISSTVLLLSNILLLGGTWWSFDQSCSATPVAVKKKHKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXZ5</accession>
    <name>A0A5B0PXZ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016109</name>
        <name evidence="2" type="ORF">PGTUg99_009490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000345">
        <property type="protein sequence ID" value="KAA1098828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXZ5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="188" mass="21103" checksum="7CB03C17C7459A30" modified="2019-11-13" version="1">MKGCDVRISRFRNPQSDACRGSAIVHNYEPSFHGKSGEIWSLGLRNLRLYDKDLPHELLAWDARTTRAQSIVGFARQRVRRVGWPWQARCANGLVRRVIPTNVRTDFARQILQSITYGLANSSDVATPDTCRTAFSDKLSDEECLCSDGATCFRVLGIELMILVSVAVDLFIFIFILDNITPVIQLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q116</accession>
    <name>A0A5B0Q116_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036621</name>
        <name evidence="2" type="ORF">PGTUg99_007736</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000309">
        <property type="protein sequence ID" value="KAA1106807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q116"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366514" evidence="1">
        <location>
            <begin position="24"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="179" mass="20326" checksum="904A7E78AF629BB0" modified="2019-11-13" version="1">MLLKSLCMAVYALNHCMFSSSLAKPVMKRSIERFGMEGSSSVEESPFHGPLYESNEYHNINGDLHIVSWYCTPPEITFHFNGKDRKVTYHEFLMLGDSDGISKSDKQAMTNALISKEQGSKVIGMSQIGNQPISAEEKNRKWPNGPPRFIKTTDMIHSNTDGRKIVYRYFRTTPVAEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1R8</accession>
    <name>A0A5B0Q1R8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001447</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1R8"/>
    <dbReference type="OrthoDB" id="10305548at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="411" mass="47727" checksum="928BA9403F6E2B66" modified="2019-11-13" version="1">MPIPHSSMIFCFLMLTRFGAICMYYPLSRLIHNPLGDFPSYLSPEQQNLLRGRAWGKINDEEWAEAQNMQIRKLGFFPEDLDPVNKEWLYGKIYPPSFRHVSPYEVDRNLGSLSPRGYRNSDRMSREFRMGIDPENSSQGFDESYQHRYLHDSNSFFFGDASHKRYSESSLKEVVRNKNDPSELTLDQLDKKGWRFPKGLAHQSQFDKMPVLMDLKKALQAMREFHRRGAHTEGSTVSSSIKGNKLKGNWDHLDHDMGKLEIWENAHKDKRTRFESGTTEIIPPCKITLEETWDYIYKALQVTHFLPQDEKGHLFFANLQILLADALEIGTKQEQKTSFAQRFAANLRPLLAQPQFPRLLSEASGREILRPMDRLSKWLKQNAELEFSNCEEKSTHTDKIPQAIPSVLVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q249</accession>
    <name>A0A5B0Q249_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q249"/>
    <dbReference type="OrthoDB" id="2508202at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15258" checksum="5B2C249DEC99AE22" modified="2019-11-13" version="1">MYLFSLLPPSTQFPPILNSNLISSFYKLESAMQSAKFSPVFTTILLLCAVASVASFGCSFGSPCCVEFTSSSQRTLRLAEQVSPEPKIYSCSGQPMSQDTRCCDAQYLPPQNPDGSRTVDISTYNSHCKPAGPNPPPSTSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q3H7</accession>
    <name>A0A5B0Q3H7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014511</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q3H7"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="414"/>
        </location>
    </feature>
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        <location>
            <begin position="445"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="661" mass="71277" checksum="0E6A70BDC9373717" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5S7</accession>
    <name>A0A5B0Q5S7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5S7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022887111" evidence="1">
        <location>
            <begin position="22"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="58" mass="6096" checksum="D0642DB93F9AAFBB" modified="2019-11-13" version="1">MKCLIILEAVIAAVLLQATSGTQIDASGPVCSKDVTPFCSHCLSPDHTKLSCPLLKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q832</accession>
    <name>A0A5B0Q832_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_032057</name>
        <name evidence="6" type="ORF">PGTUg99_027716</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1106256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1109307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q832"/>
    <dbReference type="OrthoDB" id="27832at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Nonsense-mediated mRNA decay factor (Upf2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000113">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="488"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="698"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="442"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="927"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="450"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="934"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1012"/>
            <end position="1025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1106256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1109307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1147" mass="128835" checksum="2BF79897183E0667" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAB8</accession>
    <name>A0A5B0QAB8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011254</name>
        <name evidence="2" type="ORF">PGTUg99_005186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000445">
        <property type="protein sequence ID" value="KAA1078930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAB8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366525" evidence="1">
        <location>
            <begin position="16"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="52" mass="5793" checksum="0E91EAA9546FBCBC" modified="2019-11-13" version="1">MVSWFIILLLGYNLASKLPQTLDLSLDPMHTAEVAGTSNSFQHSINPTIYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCD4</accession>
    <name>A0A5B0QCD4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031554</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCD4"/>
    <dbReference type="OrthoDB" id="2509905at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="385" mass="44198" checksum="26EE7F4AFD2D3637" modified="2019-11-13" version="1">MSGLEDMDGREALAADQILHQAAFAANTFERFGQLDFASRCDLVADLSIDRLRSKKFLLIKLRSGLLPQLRQHIISLKQALWHPNSVLSNPTCILKFVIETQPKLEMTLDRILWIISDIIRGRIETTNQTNDQHFKEFKPYVLRGLESSIRNGLRSALNFFFDVCRQLARQVVFPGIKQTYTETSVDALLESIECVVRWSKGSELHYIYDQWKLGVQSFDYTLRTLLLGANPKNGFYSEPACKAAQKFIPLIKLSQLFFKKLTTDGVAKKDLPFCTEMSSQQLSSLERSAEEIERRISNLVFHLPRAFQDNEPFPVQDSIKQIKALMNEFQPCLLLAALYYIPRCTETNVCSSEISLKTWIVTWNTLFHQATHNAIEICESFETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEN4</accession>
    <name>A0A5B0QEN4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamine synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEN4"/>
    <dbReference type="OrthoDB" id="77835at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF12">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="19884" checksum="45A4A65E2077FF4C" modified="2019-11-13" version="1">MSSKPKSETIDELVEHWKRRLDTYDKVKLAGIDADGVLRGKLVSKNKLLSAIKSDGLGWCSVIFGWDIHDRTYDPELKISNSQNGYRDLRARVDLESMRYVPWELKDLDCTDNYGTPFFLIDFYDPSDPKTPLCACPRGLLKTVLAKLKNQAGMVALAGIEVRATFLLSLRALNKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHB9</accession>
    <name>A0A5B0QHB9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHB9"/>
    <dbReference type="OrthoDB" id="10274865at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022769546" evidence="2">
        <location>
            <begin position="20"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="220" mass="24740" checksum="67718D76BFE129CA" modified="2019-11-13" version="1">MLSSFWIIGLMFSIIHVEGLVQGPMEKLIEYPFMRLCGDRVDKGHIFVEYTSKMPSSDVGQADFKNIRLKTEDITMALAELEATNHRDSAMQDLAEFLAFDKAAGFKINYVVVDTVRARDGSSLDHWTWNSSSHFYCFKSLAPEPAQLINQICTQKISQILFPEAHTLSSSSKGPSGERMQEQDQHGLNLSKDMDLKSLKVGETKNSDNIIEPAPINHEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHC3</accession>
    <name>A0A5B0QHC3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHC3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="62" mass="6996" checksum="64EFDE13FAE91DCF" modified="2019-11-13" version="1">MVIASQPLKTAHVARHRWGRAHSMRSEKGGEYWRRQATAYGTLRLPAPTPAPFLDAKRLQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHU7</accession>
    <name>A0A5B0QHU7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHU7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="65" mass="7565" checksum="69A43A7188155CA5" modified="2019-11-13" version="1">MIEDLKSYKWMRLKAQVILRPFGSNKRKNTAGTFPSENLNSDDDETDVDPDDPDEQIQFQGFMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIU9</accession>
    <name>A0A5B0QIU9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIU9"/>
    <dbReference type="OrthoDB" id="10307106at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="174" mass="19825" checksum="AFDFFE43BB216033" modified="2019-11-13" version="1">MESRHEDQINNLKAKHKEILVASLTELDLLRTRKFESNLSALQEKHLAEINEIKLDHQKQIKAITANPETSGGPKSGTPDGDSMITYPSELAKIKSSYEEKIEMLKLQHQTDLNALEEKFLMSSLSADLRKEQQANQRKINLLTDELERHPRDPSTQHSTAASSHILFLLHNRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QK48</accession>
    <name>A0A5B0QK48_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QK48"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="12519" checksum="5CCDE922133939A8" modified="2019-11-13" version="1">MDKDFKLELNKKRRDQESAQKAASGDTTEEAMIVDDTIDQLGTVMGDDNPVDDDDSGNETEDEVKGFPRGTFQVEESDDEEAELNWMDFVGVAAGQINLEPEDLNESADSPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMF2</accession>
    <name>A0A5B0QMF2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004703</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMF2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="61" mass="6924" checksum="0BD018598D5582FC" modified="2019-11-13" version="1">MAFLVRFSIRQSLVASYVSGPLRINSSIQPTSFGRVVAWGSTAYGSFLLFDQTFQQFEYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ78</accession>
    <name>A0A5B0QQ78_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Decapping nuclease</fullName>
            <ecNumber evidence="6">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RAI1_6</name>
        <name evidence="9" type="ORF">PGT21_034893</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside-ribonucleotide in mRNA + H2O = a (N(7)-methyl 5'-triphosphoguanosine)-nucleoside + a 5'-end phospho-ribonucleoside in mRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66928"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17313"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:172876"/>
            <dbReference type="ChEBI" id="CHEBI:172877"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:66929"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60880"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:60881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78683"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78684"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DXO/Dom3Z family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ78"/>
    <dbReference type="OrthoDB" id="2498214at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034353">
        <property type="term" value="F:mRNA 5'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110155">
        <property type="term" value="P:NAD-cap decapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013961">
        <property type="entry name" value="RAI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039039">
        <property type="entry name" value="RAI1-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395:SF9">
        <property type="entry name" value="DECAPPING AND EXORIBONUCLEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395">
        <property type="entry name" value="DOM-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08652">
        <property type="entry name" value="RAI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RAI1-like" evidence="8">
        <location>
            <begin position="87"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="582"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1115299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="633" mass="71997" checksum="DB14A96D49A6A004" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQC4</accession>
    <name>A0A5B0QQC4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">GET complex subunit get1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GET1_2</name>
        <name evidence="9" type="synonym">GET1</name>
        <name evidence="11" type="ORF">PGT21_036151</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WRB/GET1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQC4"/>
    <dbReference type="OrthoDB" id="69461at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000006">
        <property type="entry name" value="Protein GET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03113">
        <property type="entry name" value="Get1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028945">
        <property type="entry name" value="Get1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027538">
        <property type="entry name" value="Get1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650:SF1">
        <property type="entry name" value="GUIDED ENTRY OF TAIL-ANCHORED PROTEINS FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650">
        <property type="entry name" value="TAIL-ANCHORED PROTEIN INSERTION RECEPTOR WRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04420">
        <property type="entry name" value="CHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Lumenal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="2"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1115408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="201" mass="22776" checksum="1D0C481D88EDC1BB" modified="2019-11-13" version="1">MLGFYIFFLVASAQFIQWIGKTKLIDWTHNLYLWISGSSLLKDQANLKTQILKTKVEFQATSSQDQFAKWARLRRKIDKDLVDLEALNKEISSAKVQFAALFSSLLWTFTSGFQFVLISWYRRSPVFFLPQDWFPSPIVWILSFPSAPYGAVSTTVWTIVCKRALALSGAILVDTFGIFSEPPPVAQESQTPASKVPPSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSU1</accession>
    <name>A0A5B0QSU1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSU1"/>
    <dbReference type="OrthoDB" id="2506836at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="627"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="650" mass="71717" checksum="C1B8133E3B98EA7C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTL5</accession>
    <name>A0A5B0QTL5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTL5"/>
    <dbReference type="OrthoDB" id="10322018at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="150" mass="16821" checksum="5B84217DEA8F7FE1" modified="2019-11-13" version="1">MPLIILSHNKLARLGLVWALIFLGHLVLMGLGMQQGNPGRTCTSCVARHKRFSLVVPSWQESSQHPVHPQRHDTLLCSIFQTKETCHCSIPVQLHFCDRCDMYAWMPTGHCQDHPHLQPGPVYCNSDNIDLLRQKAAAAAPVDTTLRLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QW64</accession>
    <name>A0A5B0QW64_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013634</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QW64"/>
    <dbReference type="OrthoDB" id="10298933at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="363"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="564" mass="62914" checksum="E64B1EFF0A20AB73" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWA0</accession>
    <name>A0A5B0QWA0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021961</name>
        <name evidence="4" type="ORF">PGTUg99_036828</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWA0"/>
    <dbReference type="OrthoDB" id="2507450at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138025" evidence="2">
        <location>
            <begin position="26"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1117183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="333" mass="35606" checksum="F0EEE0EBE552D326" modified="2019-11-13" version="1">MMMLRNPGLISFICLIHSIQAITRARVPRSLAFAGGAKSGGHLLLLPRKISDSNSHPQVLQEIHNLAGCGGEICGTLAGDAVQPLLAGADECAQQDMADKMIDIAKSKLQDKAVQKKLIELAKTYRQTERNTFPDYSSPSTPDRNSLYCQKKPKNPELVGLSQKQSSAADPKLFFDPKSNGKSIQKGSDPRTEPLGAAKGQTTPVNSTASTKEDTDDPDVDEDDVPEDGSDEAAGNASDNSANASSPGPNAFSSSTTRSPTQVQHDRELGIVESSRPHKLADSTQSSQAPTSVRPQAPSTDPVKNSATKEKSQPPQGKRKSSGKCRPKRLRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWD7</accession>
    <name>A0A5B0QWD7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWD7"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="221" mass="24128" checksum="3870914547B947CD" modified="2019-11-13" version="1">MQDHHMELEKGSNHPFLFPGAAPTTDSKEAALCRVVVNSGKMILLDKLLTCPQASQLQQSRVDDQSSAEKTTQNASSLWPADPEKSQPTRQESLQIVPPSSSAIEPQVLPTDVNPQSPTDRSASSSALQKSAPDSTTEREPEEPMEIDEQVPPSGMPADPLSQSSRSERSARTARSDKRKRSHYETDQQTNSALSDIEEEGSGRQNKRGSRRARDHLSASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXM3</accession>
    <name>A0A5B0QXM3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXM3"/>
    <dbReference type="OrthoDB" id="2507899at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022965932" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12782" checksum="2736DA77741DD413" modified="2019-11-13" version="1">MQLSNVFPTSLVFLLQGAVTYGQGFGCKGNVMVSYWPNSGCVKMTGMKNELAEVIPAHWNNQASIYECASDKNHYLTEACCSNDQAMNQKIPSTTFLINCRKPNGKQFKWPLPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYQ9</accession>
    <name>A0A5B0QYQ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYQ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8539" checksum="A58423640DE7B8ED" modified="2019-11-13" version="1">MTSRPISRKRLHVLYTAPLLLGVTCLVLIIFSLGGLVPWKQTSGTIMVIHRTSSDAQNNNTASFHFGLLGQFLPRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZW1</accession>
    <name>A0A5B0QZW1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Karyopherin transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CRM1_1</name>
        <name evidence="9" type="synonym">CRM1_2</name>
        <name evidence="8" type="ORF">PGT21_006973</name>
        <name evidence="9" type="ORF">PGTUg99_023126</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000039">
        <property type="protein sequence ID" value="KAA1135415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZW1"/>
    <dbReference type="OrthoDB" id="27218at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000022">
        <property type="entry name" value="protein EXPORTIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1067"/>
            <end position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1118818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1135415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1087" mass="122907" checksum="FDF4C0BDF35FF155" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2T1</accession>
    <name>A0A5B0R2T1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2T1"/>
    <dbReference type="OrthoDB" id="3132747at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="31"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="310" mass="35519" checksum="B1F3C37A120D3700" modified="2019-11-13" version="1">MNAGRPVLLDPRNPESVIAIMDFTPWDRLTETDKANLEFVSTFLHQTKEFVNPVSSSTRSWGGKMWGIGWRKSQDFLQIFGRYIKQFPPNKMEKYDTLFQKSKRVGEILGEYYKNLASGAFKNNQDLMKLFNLPSFDSLSFGEKPSPNSCSPHLTFTTNNFFNPPHKDHNDMSKWAFVMFIPIYSATGKLAGPDSGYDLKGGPFVFPSFHTGISFDKQHGIVRMIWQANKYRHCTLPYLSPSGSTEFTRLGLSLQITTSIVDAVKREAAGYYTDPLNYFGDHFYYMFRCLAKGTLLAALFCFHYFLFIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R343</accession>
    <name>A0A5B0R343_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_024179</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R343"/>
    <dbReference type="OrthoDB" id="8120565at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF14">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="399"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="85"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1119395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="583" mass="65213" checksum="4CE92C598FD5BCC8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3Z1</accession>
    <name>A0A5B0R3Z1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_037317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3Z1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1120256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="36315" checksum="DB52A0EAA24740F6" modified="2019-11-13" version="1">MTPYYDAKASTTNDSDTNTKPKGRPFFILYNVLARICREGKKESQCQKLKSDHFEPIMMNIHDLNYTRLKRLVIEHIRKQSQQQSPPYPIDVIAVYAEYESALQWYYAAVPKSSPIPVDFVHTALTESSFDAFVKEVENAPDDYRLWIEICMCSLNKIEKPEAIISIMVSRPIVKAPHGQRSLQITFQAPDQESQPYAPTTRPLSAGVAILDLSNCLIDDVSYVPIKRDSESNNQSPAKKRIRFNESTSNMSLADFLGLCSIPIDNKHVREMIETHKILDWQVFIGTSLTELTQLGFKWGPARSILAGVAKATESREAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R427</accession>
    <name>A0A5B0R427_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005993</name>
        <name evidence="2" type="ORF">PGTUg99_019115</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000244">
        <property type="protein sequence ID" value="KAA1120441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R427"/>
    <dbReference type="OrthoDB" id="2341552at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="876" mass="99518" checksum="B1875F6432B25D88" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R7P9</accession>
    <name>A0A5B0R7P9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036812</name>
        <name evidence="3" type="ORF">PGTUg99_030262</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R7P9"/>
    <dbReference type="OrthoDB" id="2504450at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="111" mass="12501" checksum="8C81C483AFAE5452" modified="2019-11-13" version="1">MTNRYACPHLSTCNDTKYNKKSCHEHSGLGLERVKAHTLNIRIHGNCQALCPVYKLDDVFEFQKFTHLDTTLGPTRSSRGISSELAPSFRNKKATKAPKVVPSNMEKRLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RFK4</accession>
    <name>A0A5B0RFK4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SGS1_122</name>
        <name evidence="8" type="synonym">SGS1_55</name>
        <name evidence="8" type="ORF">PGT21_017785</name>
        <name evidence="9" type="ORF">PGTUg99_027150</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RFK4"/>
    <dbReference type="OrthoDB" id="2503963at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="61"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1107538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1124661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="267" mass="29994" checksum="74D8D9DB16C3CC54" modified="2019-11-13" version="1">MIRPKKVAPKTRSSPQKASTGITALKKLLEKNDDALKSEIATRAKNYYNGQDPKPLQVETVATLARGKSTFLLAGTGFGKSRIAEMYFKILPPKSKPVVLVLNPLDALGDNQVEEKKGKFTAINLTKLTFNPLVAEQIKAGVYNFVYLSPEIFLNSKLWDLVYFSSEFQERLGLVVVDEAHMIYIWGLVASTNGTKKYSILVRHQDIGIFRPLYGDLARHLQCRNNIPILLLSATCRPVAVDAILRSLKLTKDLIKMLYEELTRKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RHV0</accession>
    <name>A0A5B0RHV0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020649</name>
        <name evidence="3" type="ORF">PGTUg99_002565</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000178">
        <property type="protein sequence ID" value="KAA1125396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RHV0"/>
    <dbReference type="OrthoDB" id="2499550at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="366" mass="40745" checksum="1D4180737F6BC59B" modified="2019-11-13" version="1">MSLNSSIGLSRKPSQRLLSAVRSIKQKVNRPKGQPKDANSLGTRPLSETILDINFQGGSSTSTTANCRPTCWPMLAPMMFEFEILPRFYRAAPYSSSHQETDTARSVPYGQNVQSTNVNHRNAQEKRNVGVFRVCQVSPTLSRCLPSTPPLGFGKLGDQNKPEQPLPRLFCPPPRCDRTDSLVQPPCSPYRSSEDSSSSQESQELDYASRVSIRLSVEIVQSFFDHTGPSSPCSESSDEELYAEKISARLENLCYDKPLEFIHRRSHAGKGNANKRISSFGVARIIISSPNALQKPLLSPSFKSWLDKFIESRLNQDESLNGFHDSFESMIPEETHSAIQEEVCEEMNPVPVLKANHDTPNSLDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJ58</accession>
    <name>A0A5B0RJ58_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase</fullName>
            <ecNumber evidence="3 12">2.4.1.131</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ALG11_2</name>
        <name evidence="16" type="synonym">ALG11_1</name>
        <name evidence="16" type="ORF">PGT21_013592</name>
        <name evidence="17" type="ORF">PGTUg99_005782</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="17 19">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:29523"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="EC" id="2.4.1.131"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11 12">
            <dbReference type="Rhea" id="RHEA:29524"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.131" evidence="3 12"/>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000182">
        <property type="protein sequence ID" value="KAA1125158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJ58"/>
    <dbReference type="OrthoDB" id="2276068at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004377">
        <property type="term" value="F:GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03806">
        <property type="entry name" value="GT4_ALG11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000256">
        <property type="entry name" value="GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038013">
        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031814">
        <property type="entry name" value="ALG11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919:SF1">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15924">
        <property type="entry name" value="ALG11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="ALG11 mannosyltransferase N-terminal" evidence="15">
        <location>
            <begin position="69"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="14">
        <location>
            <begin position="356"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="290"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF15924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1105623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1125158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="568" mass="64387" checksum="D4C1EE9933EC0652" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RME9</accession>
    <name>A0A5B0RME9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027469</name>
        <name evidence="3" type="ORF">PGTUg99_022678</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000169">
        <property type="protein sequence ID" value="KAA1127111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RME9"/>
    <dbReference type="OrthoDB" id="10649412at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1127111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="134" mass="14168" checksum="25DD884616C14526" modified="2019-11-13" version="1">MAPSESASPTLAVSPTPEMKITTLEKGSLPPMSKKTLEKGSLPPTINVILPATNTTSPLTPVIDPTLETSTLPRPKITAGNESTTSGPPEVFAPIPPPPESIYKTKEAMIESIQNWALNHGYAIVVGNSAFVRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S060</accession>
    <name>A0A5B0S060_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_035518</name>
        <name evidence="6" type="ORF">PGTUg99_009171</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000112">
        <property type="protein sequence ID" value="KAA1130154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S060"/>
    <dbReference type="OrthoDB" id="2495203at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF1">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1081428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1130154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="35821" checksum="CCB57DEC4FCAF1E0" modified="2019-11-13" version="1">MSVADQIPGETEFQMDRFGPALQHLPTISAKGFEVPYPLDDPHMSEQLDFFKSATLIEDSQELKAHILAIRDECCKAFPFPCILCFYFLQGMIVRHPYYPMVREVIQQASDTQNILLDIGAGMGTDLRQIISHGWNRDDVLGVDITSDWDTLGYKLFRDSERPIPNFFGDILSPEVLNDSAPVEVSPGRIDLRSLKSLNPLKGRSKFLILNQVFHLFDEAHQNTLAERCAMLLSDEPGSAIFGMQMGGYTKGFESKVFVHSPESWRRMWDSIFGPNKVKVTAELVDLPTTQRNTLRSLFPTSRDIVRLYWSVTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S110</accession>
    <name>A0A5B0S110_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008982</name>
        <name evidence="3" type="ORF">PGTUg99_014845</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S110"/>
    <dbReference type="OrthoDB" id="2507484at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138267" evidence="1">
        <location>
            <begin position="23"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="117" mass="10662" checksum="9C914334837D93B9" modified="2019-11-13" version="1">MKFTFGLLSVVATILSTPTVTAVPTHGLHRGYFGGPGYGGGYGGAYGAGYGGGYGGYGGGYGGGYGRGYGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGGGGLGLGFGLGVGVGVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S573</accession>
    <name>A0A5B0S573_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026925</name>
        <name evidence="2" type="ORF">PGTUg99_008574</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1132952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S573"/>
    <dbReference type="OrthoDB" id="2513458at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1132952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="119" mass="13501" checksum="AC10D771940A0F96" modified="2019-11-13" version="1">MSQIQGPLDVRITLAPIQIMWLKDQQSMINNILKKYEPAPEDQPSPLSHIDEYEQDRRAWDWHVLISGRVTAAARDMSIPEWAIPNVKAIWDARRNIYGKGPLLFTAPEAIPGQQTGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S9R6</accession>
    <name>A0A5B0S9R6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007410</name>
        <name evidence="2" type="ORF">PGTUg99_031530</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S9R6"/>
    <dbReference type="OrthoDB" id="2496520at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1134185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="403" mass="46069" checksum="36FCF8A5D734E575" modified="2019-11-13" version="1">MKNSTTRTSDGSGAQLQYLGELVIQGFTNLDHKYGRQRKSTREAPWTYPVNEVNSKEVLYERLLSTNLPRLKDQVYTILATLEPVSMKENPELSLRRVLEIQPELDYNITQIDSAFLHLCPESSSACDQTDDQHLKQFKAYNLVELRKEFVFALDWICSAFRAAPEHIKDMRLSTGGCFVDDEFDMSGHKEYTEKTVYWIQHTIIHSQRSEMDAIEEQGKSGVRTIDSLLIDLISLFTPTAPMGIARLSPLRRQSFIDLAKLTVPIMKLLKLFFKKLFHIGINGCRALVSYTQMNSEQLKYLCQSSEKVNGKLSSFMYNLSDVDTEFGDLPVDIRSLVQPLEQIASYLEGPLLSIVLYLVPLVDNLPDQTYFQSWFSIWNSQFNIAIHNVVQAAQTLDQNQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SAE9</accession>
    <name>A0A5B0SAE9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010229</name>
        <name evidence="3" type="ORF">PGTUg99_026617</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SAE9"/>
    <dbReference type="OrthoDB" id="10269915at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="310" mass="35262" checksum="F4D8AD5FBA55BF34" modified="2019-11-13" version="1">MHEEVDTHGRTRPEEVDDIEIPPLMGNTLTEAELQEFYPELAEWLCTTTASEDEGPGASVPPEVPAPLSVAIILEYHCWLPSPQADQDGCRQYRSAHGVQTWTEAIGPSRLVTWRGDPRASTWDQIRCDVLEAFITYDTEVADELRSNDRYRDLLWEGTIPDLPEFGRGVPFRICTDVCWGIFTDYIARTTATSKIYLRLFTNRPYGRTAVSDAFHRAMHIRHCPHRNNLIAELERRLHTEATHLDGNSGHIKLKVKWATGGQKFIRMSAAVIHGWARAIVSNPSLVTLDTPPVGREYLWEWVHAHAQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LI12</accession>
    <name>A0A5B0LI12_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-1,4-glucan branching enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GLC3_4</name>
        <name evidence="3" type="ORF">PGT21_000344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000200">
        <property type="protein sequence ID" value="KAA1063981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LI12"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000080">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-amylase/branching enzyme C-terminal all beta" evidence="2">
        <location>
            <begin position="34"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1063981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="130" mass="15424" checksum="F16128336AA6835C" modified="2019-11-13" version="1">MIRCYGIKYLNNFDAAMNNLESQHKWLSSSHTFVSLKHESDRVVAFERGKLLFIFNFHPTQSYTDYRIGVEWEGKYQVVLSSDEKQRFGGHDRVDLQSEYFTTKMEWNNRKNYVQVYLPSRMVLVLGLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LL14</accession>
    <name>A0A5B0LL14_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LL14"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="189" mass="20422" checksum="89B8B58B3E684A8F" modified="2019-11-13" version="1">MRSRPPSARGRYRGPIHQPAPQPGRQGAGFQSFYPIVTPPGFGAVYPQTQQPQSAHHGPLNANNGAAPANQNRPADYYRPPQYWLQRVNAPSESIGSQRPKPSVNKANADSDPQGGSVARMVELGDVADDLANIHFDHIQAEVGNNPPILLHPVTQPTSPAKGISLSLAWTTNESPYMVYYTVSSRAPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LM98</accession>
    <name>A0A5B0LM98_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034608</name>
        <name evidence="1" type="ORF">PGTUg99_022685</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LM98"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="55" mass="6121" checksum="028AAA0C2381C5C0" modified="2019-11-13" version="1">MEGKPKYSIWTAFGEGLGAGTGSLAQPRELSALVRAERQRLRAEPSLRSMNRIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMH5</accession>
    <name>A0A5B0LMH5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMH5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8567" checksum="05623AF35EEB5F43" modified="2019-11-13" version="1">MVVAGIKNKQVKIQGAAATNQLGVLNSTNNLVKEFDGQSEAITDRIAQRARHTQNHIKQHHNDIKQHSEKIERTSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMS0</accession>
    <name>A0A5B0LMS0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Myosin type-2 heavy chain 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_025068</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2500868at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin_head"/>
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    </dbReference>
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        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="54"/>
            <end position="126"/>
        </location>
    </feature>
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            <begin position="130"/>
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        </location>
    </feature>
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        <location>
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        </location>
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        </location>
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            <end position="1241"/>
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        <location>
            <begin position="1309"/>
            <end position="1371"/>
        </location>
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    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1400"/>
            <end position="1476"/>
        </location>
    </feature>
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            <begin position="1674"/>
            <end position="1705"/>
        </location>
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        <location>
            <begin position="1812"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1880"/>
            <end position="2409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="223"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1065110.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2422" mass="278846" checksum="B7DF93DB13C687A7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNH9</accession>
    <name>A0A5B0LNH9_PUCGR</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015921</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1065931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNH9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8751" checksum="857486D7A87785B7" modified="2019-11-13" version="1">MKANFCLLIQGPILHSNPQAWKSPPGHILANEGHSPEYSRQDYPEFSQPLVANEGKMKIPFDTIHRQFARIANNKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPW9</accession>
    <name>A0A5B0LPW9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004360</name>
        <name evidence="4" type="ORF">PGTUg99_020879</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1080009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPW9"/>
    <dbReference type="OrthoDB" id="2506918at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="141"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1080009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="207" mass="24171" checksum="D73852B3689D8123" modified="2019-11-13" version="1">MSHNLYKFHYGCQLPSTKSRSQKTIRLAFEKRTAAEIGQRLKRTINRNRQNQQNNTSQSGAANHHDIQADDFDNEHNELGFNETSLWEDYKHILNNEDKASRARMRLLHQELLQQQKDRNWKDILDALFPTYLHMKKLTANWTLPSAFDNFSALVCSCSPDKYRSREIDLVDLMDPVHLLANGYLASTPVFPQTAFSLRLLNFYDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQL4</accession>
    <name>A0A5B0LQL4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_023265</name>
        <name evidence="3" type="ORF">PGTUg99_018061</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQL4"/>
    <dbReference type="OrthoDB" id="2502552at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1066731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1071934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="116" mass="13008" checksum="2F458DAD46727A46" modified="2019-11-13" version="1">MSPIIIDHLMDLENLKTNFARPQALYETTPSTSHTPLITAKTVVILIDIIIVVVISLLLLLGCYLYRRQLKRKENFTKSFETSEAKLAAESNRAAMESRSMCPSTVVGDEESTHKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSU2</accession>
    <name>A0A5B0LSU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSU2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022718814" evidence="1">
        <location>
            <begin position="19"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="605" mass="71365" checksum="0C1E9433E9669AA8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUV2</accession>
    <name>A0A5B0LUV2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUV2"/>
    <dbReference type="OrthoDB" id="10322983at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="161" mass="17759" checksum="95C403FD8F441EB2" modified="2019-11-13" version="1">MEDDAGCPSGHICSALTAVWEAVQVRADHAQARGLTSIPRELAPVQLQRSDRTKEHTIMSNMPDHDQPIPDSDITQTTMLNTQVRREKEAHGIGDCPGNVRANDRGRYERNRMRREWLHEATPGAQSFGRPPADEERTLADDSTIGPEEPDASSDAPSVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVW2</accession>
    <name>A0A5B0LVW2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVW2"/>
    <dbReference type="OrthoDB" id="10292445at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="382" mass="44923" checksum="2EDCD76CBE67F530" modified="2019-11-13" version="1">MLKYCPNFVGTLLIFNRLQVFSNQTPMIAESSTKAAAIVRPKYWFPLPQRSDYMNDEIYDLSDWEAGTSLSHMPSHGETSEAKVKLEPGSFRGNDRRILYYHQLQNSDGTADHVNYLIQKACQYFWEKPEARREEVRTWLEMFAYHSKRHRRHLFMPTQASLLYGLQNLRKVYAEDPMPVPHIDSVLKLIKRRIGITENGPAGEVSFNWPWIQERINPSMGLTDAMKSYREFQEMIKGEEKIISVLHFTRKKKENIYHNFLKRLSKARREKKRSEFTQAVRNVIQEFELSTSQSRLATLSLFYHTLTQHKTSRLRREAWSIFQEFEHTHGPGFLYHESQLVEYIKRETEVVKTPDHQGTEPSPSQTHFTDSDTTEEPAEDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWS1</accession>
    <name>A0A5B0LWS1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024015</name>
        <name evidence="1" type="ORF">PGTUg99_025330</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWS1"/>
    <dbReference type="OrthoDB" id="5392716at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="165" mass="18712" checksum="B5A7CB71409B1C3E" modified="2019-11-13" version="1">MNLNEIQARLANKTSVNVSIRTIKRYLKQLNLKLLRNDVTHGKVTIQQVYDAINDARTRRLESDAGYRRMRMILMREYDIQIPHPTVPEPEPRGGSQSLGSTLIARITLLCPEPTRTTLLARNGLKIDIAFSPTAPEPARTTRSLATKRLKSNIASQQVLNLRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXM9</accession>
    <name>A0A5B0LXM9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXM9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="327"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="606" mass="65836" checksum="D6F4D5A75466FB83" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LY76</accession>
    <name>A0A5B0LY76_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G protein-coupled receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007727</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LY76"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="48077" checksum="6C7CA0D7EBC606E8" modified="2019-11-13" version="1">MNQVTQELIKFLTSLPASTNPYEATAVKIKQETEEATPVLWGLILRRIIAFQFFVLCCQAAAVLYLRKKAKKLHFFRYNKLGLIHVEVLNEIVLLMLVFSLMAFVDLFTQEFTEKNLLRLSSKIVLHTCKFPIAAIVCWCKISKILLDLVCLECIETALNLSSLGARARAGASIRLSAHLRFALNGSLVTLMVVPGALLLWISILVARSLRIIETNTDGIVATLIETAPSYRAETYSYLSVLRLLKPMASTLEIATRLNRYTKIVLLLYLLQHLLIAIVYLPISVIALRGLRKRAVPKDLLQRGSVQICTPTHQDTIDKVRKRLINHALLIYLQEILYCPTLVYMLCAPSTKLTHFSDPTWVLVEQVAIHGPIALIGNIILSFLIQNALYAARSSCNHLHPVDTDTRPKSVLEIKLKTEQHKETCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYM6</accession>
    <name>A0A5B0LYM6_PUCGR</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYM6"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="612"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="577"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="870"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="957"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1115"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="381"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="639"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1244" mass="139850" checksum="2CEA06C0AEB021C9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2P2</accession>
    <name>A0A5B0M2P2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013757</name>
        <name evidence="1" type="ORF">PGT21_013978</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2P2"/>
    <dbReference type="OrthoDB" id="2505079at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="54" mass="6018" checksum="49EF9CED40EECCB6" modified="2019-11-13" version="1">MFPILIVVKMDAVNPTILKTTIEAIPVLTEENFSSWKTRITALFKLGGVELLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3K4</accession>
    <name>A0A5B0M3K4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025555</name>
        <name evidence="4" type="ORF">PGTUg99_011562</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000078">
        <property type="protein sequence ID" value="KAA1132598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3K4"/>
    <dbReference type="OrthoDB" id="2507414at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1132598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="169" mass="19157" checksum="34A3EB5E51F0FE40" modified="2019-11-13" version="1">MSPYPQPLPGPSTSNRKKEAVKFSTLDHPPRANLLPPAALLSLLCLAFLCLLFVCFRHTEWIVKARKKVWTLLKEQANKLSDQFEEFKYNRQRRIKLLDDDDDDGQEEGEGRAPTMGSGGGSRLSSNLQSLNLSHHHRESGIDPLTLAELDEEEEDEDDEVLPQVLYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M658</accession>
    <name>A0A5B0M658_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">UDP-glucose-4-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GAL10_2</name>
        <name evidence="11" type="ORF">PGT21_023402</name>
        <name evidence="12" type="ORF">PGTUg99_004821</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M658"/>
    <dbReference type="OrthoDB" id="9402762at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005996">
        <property type="term" value="P:monosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725:SF47">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="10">
        <location>
            <begin position="56"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1066158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1072637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="140" mass="15639" checksum="73BF1A1F7191EF4B" modified="2019-11-13" version="1">MALVQSLLKLPVFISLDRRSLELDKLRFSFHNSFPPAIVRVSRIALSELPNDASEDDKLYTNVTLYKGDITCKADIEKVFETEKVWGVIHIAAHKAVGESGEKPIQYYENNISATINLLDVRSLENGFVCSEPTVLTLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6D6</accession>
    <name>A0A5B0M6D6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_018107</name>
        <name evidence="9" type="ORF">PGTUg99_020022</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000174">
        <property type="protein sequence ID" value="KAA1125915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6D6"/>
    <dbReference type="OrthoDB" id="19092at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120104">
        <property type="term" value="C:mitotic actomyosin contractile ring, proximal layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000045">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF7">
        <property type="entry name" value="NOSTRIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="7">
        <location>
            <begin position="45"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="11"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="377"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="465"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="473"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="511"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1071743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1125915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="524" mass="59769" checksum="085934B696FB0053" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6T9</accession>
    <name>A0A5B0M6T9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031076</name>
        <name evidence="3" type="ORF">PGTUg99_003999</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6T9"/>
    <dbReference type="OrthoDB" id="2517944at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="177" mass="19052" checksum="024D1BC5A54375D4" modified="2019-11-13" version="1">MGHNPHRNAAMGPAPNSAPAAVSTFPRSPPLSAFPAASPPFHLPSRLRPTAQVTRSNAIRRPAATHLNDIDINGDLVNKALGLTPTSPAPPLPLTSIPPRKTSSLDYSTLPLPPLPLPPTPPPAAPLLPLLPFPRPPRRARVNSNRHAMIILVPQELPQELRDIQLDAWMTRDDDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6Y3</accession>
    <name>A0A5B0M6Y3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6Y3"/>
    <dbReference type="OrthoDB" id="2513278at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="540" mass="62148" checksum="4B8022178DEAF1A0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9S1</accession>
    <name>A0A5B0M9S1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000160">
        <property type="protein sequence ID" value="KAA1072770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9S1"/>
    <dbReference type="OrthoDB" id="6507260at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="512" mass="58218" checksum="1E4F8B65E6B8D3BA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAK9</accession>
    <name>A0A5B0MAK9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAK9"/>
    <dbReference type="OrthoDB" id="2505988at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004242">
        <property type="entry name" value="Transposase_21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579:SF1">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02992">
        <property type="entry name" value="Transposase_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="505"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="510"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="975" mass="110133" checksum="0DCB1A8CF8B47882" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCI2</accession>
    <name>A0A5B0MCI2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">POL3_5</name>
        <name evidence="25" type="ORF">PGT21_024364</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCI2"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05777">
        <property type="entry name" value="DNA_polB_delta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05533">
        <property type="entry name" value="POLBc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 20" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="17 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14 20 25" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 20" id="KW-0408">Iron</keyword>
    <keyword evidence="16 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="23">
        <location>
            <begin position="200"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="22">
        <location>
            <begin position="501"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="24">
        <location>
            <begin position="969"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAA1073748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1065" mass="119658" checksum="D83C54A7818E6ABA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGK7</accession>
    <name>A0A5B0MGK7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vacuolar import and degradation protein 27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">VID27_1</name>
        <name evidence="3" type="ORF">PGT21_032134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGK7"/>
    <dbReference type="OrthoDB" id="10251113at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="VID27-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="2">
        <location>
            <begin position="240"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="570"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="595" mass="65635" checksum="AD2ECF7CA5C16ACC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHZ8</accession>
    <name>A0A5B0MHZ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036414</name>
        <name evidence="2" type="ORF">PGTUg99_037575</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHZ8"/>
    <dbReference type="OrthoDB" id="2496215at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366145" evidence="1">
        <location>
            <begin position="22"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="165" mass="18200" checksum="2A43705317A0FA90" modified="2019-11-13" version="1">MLLRRLSAVLIASTQLTSTIARPQGLFDTRLDGAGDNQRFTDLVNTMNIGLAKVKKANGVDQASVYYIIADMVFMVPQIDLAVRAIGQNAARVTAGADNIVIGNTVQNVSDDNFKIGKTLFEMWFEWMHPDVINQYIQVLESEFMNNSNQFAGVQALGRQDTTIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIN9</accession>
    <name>A0A5B0MIN9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_030374</name>
        <name evidence="3" type="ORF">PGTUg99_029619</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIN9"/>
    <dbReference type="OrthoDB" id="10300859at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137230" evidence="2">
        <location>
            <begin position="32"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1091288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="562" mass="61022" checksum="028EFAB0145965B8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJ84</accession>
    <name>A0A5B0MJ84_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028579</name>
        <name evidence="2" type="ORF">PGTUg99_023376</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJ84"/>
    <dbReference type="OrthoDB" id="3264316at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47611:SF3">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47611">
        <property type="entry name" value="HAT DIMERISATION DOMAIN, C-TERMINAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="83"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="177" mass="19641" checksum="0614B28E291A59F0" modified="2019-11-13" version="1">MHLFELAFGAESSEVTECLNLLKQEFQLVQDEQKRNTEKTPNPDLIVLDNAPVDQPTSLMARLAMCMKQKPAAQEDEIAAYLKANLNFKKGAIDRKATPLKWWKANQSTYPTLAIIARAYLGTPGSSCSVERLFSAASDVCSSRRGRLLPSTMSHCVSSLMWLREEVGLTGEFQEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLU1</accession>
    <name>A0A5B0MLU1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_022138</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLU1"/>
    <dbReference type="OrthoDB" id="2410195at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="6">
        <location>
            <begin position="72"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="208"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="502"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="513"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="563"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1077855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="574" mass="63362" checksum="5149C173AC1E1110" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMP6</accession>
    <name>A0A5B0MMP6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030192</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMP6"/>
    <dbReference type="OrthoDB" id="2501630at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="682" mass="77408" checksum="82D079C5E03F2F82" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MN94</accession>
    <name>A0A5B0MN94_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MN94"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023028458" description="Secreted protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="15864" checksum="270BB32C44D65E4B" modified="2019-11-13" version="1">MIPNLFCTKLIQLGILLSLILLSHLAMCAGRGQPNNPGNVGKRCGNCLDLHLQGRGPAIPLQQRTQLPQGIFETLSCAILQSPQEGCHHDIQVQEFQCRLCGWYAWVPTGDCPVHPRAPPIYCDSNDINIIKQGEAVVDTELRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MX05</accession>
    <name>A0A5B0MX05_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase rbbp6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_033433</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MX05"/>
    <dbReference type="OrthoDB" id="106784at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01180">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51282">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="11">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="10">
        <location>
            <begin position="243"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="341"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="120"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="433"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="799"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="202"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="388"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="433"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="493"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="836"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1081307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="883" mass="95857" checksum="4636809374D2F5D2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1D2</accession>
    <name>A0A5B0N1D2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1D2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="110" mass="12190" checksum="D0A5CF90A281177D" modified="2019-11-13" version="1">MNTKKNKAPNHTKPEDFGLYSSAAFNSQKRLCKDGVSAESDQDKASPKLAKHHTRLELSKEKQHRPTPTKGAGRAALNTYITLPQQSTKTSLATRISQLSTEQRSDSRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2G1</accession>
    <name>A0A5B0N2G1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_009487</name>
        <name evidence="4" type="ORF">PGTUg99_033082</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2G1"/>
    <dbReference type="OrthoDB" id="10257697at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF76">
        <property type="entry name" value="CALCUTTA CUP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="3">
        <location>
            <begin position="153"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1083427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1094093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="275" mass="30209" checksum="6562BC045ECA5963" modified="2019-11-13" version="1">MMPTPVPPSPSELAIPKVLFKGKLVSGKKSNSPFFVHQRHKQSRSIDNISNPPTITTTSVSPSSSSSSSSSSSSSEKQKKMTSKMKKSKTIDQHSHSILAKSAALLVSGIRYSILILLISILLSRMITEHWLWGYDGKWARLSHYFPGPQETFTLERLALHDGVQDPSKPLLLAIDARVFNVSSNPAMYGPGGSYHHFVGKDASRAFVTGCFKSGLTFDLRGLNERQKKSLDYWINFFENSPKYSKVGSLILPDLDASNTPLPVDCDPKLEGGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3S3</accession>
    <name>A0A5B0N3S3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_006626</name>
        <name evidence="7" type="ORF">PGTUg99_035135</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3S3"/>
    <dbReference type="OrthoDB" id="2496852at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="102"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1083492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1094026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="46734" checksum="A52FB03D2C35D72B" modified="2019-11-13" version="1">MASEMYTLFNKNPINTSSNSSVWDPATMHIRCICHKLALIVNAGLAALSLKTLPPGKTKQSVLGFFPILGRLNEEDENDPLLPQPQPTEANEMQTQLSEVVAADGSQRDSESDYGNTDNKGSDVTVSNESEYRSDSDLAEDEPLTHGKQSHRKKHIKSTKLLDLTTKLDVVIKKITRSAAQGANFERIAQQLDLKVSPLIAGYGIQWNIKFQSYKKAVAAKDVIDQILKEDQQANGAGDFGNVLFSPRDWTEVENLNQELEVFVELNSQMEGNSATGTHVIPKYLELKENLSEKLSLALEKDLLYPMYHAMVKRVSRYLDEAMGCSTLVIATIMHPCYCMHLFELAFGPGSSEVNQCLSWLNSKFQRVKEERKLAASTTTDANITVINPQPKNQSTSSSLASPLACHKNQHLKITRLRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5C3</accession>
    <name>A0A5B0N5C3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012010</name>
        <name evidence="4" type="ORF">PGT21_013147</name>
        <name evidence="2" type="ORF">PGTUg99_029523</name>
        <name evidence="5" type="ORF">PGTUg99_031035</name>
    </gene>
    <organism evidence="2 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000002">
        <property type="protein sequence ID" value="KAA1138610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5C3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137390" evidence="1">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1108445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1138610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="60" mass="6892" checksum="8BB91F3AA4D38999" modified="2019-11-13" version="1">MAIARRRFAHLHMFIMAWLTSCSATETCEFRKHQRTGCKQITCDTLNYQDSALKKGVTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5Q3</accession>
    <name>A0A5B0N5Q3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5Q3"/>
    <dbReference type="OrthoDB" id="2504266at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="565"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="697"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1041"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="586"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="747"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1053"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1074"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1094" mass="118202" checksum="8251A9C763037983" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7N6</accession>
    <name>A0A5B0N7N6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7N6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="52" mass="5547" checksum="3E185CB46F08C406" modified="2019-11-13" version="1">MDPLGLENLYSLKADKTQPSSMLAGRNSAPVIGMISSDALHDRTQQAAFSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBQ5</accession>
    <name>A0A5B0NBQ5_PUCGR</name>
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        <recommendedName>
            <fullName evidence="8">Oxysterol binding protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">PGT21_006311</name>
        <name evidence="5" type="ORF">PGTUg99_003489</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1118370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBQ5"/>
    <dbReference type="OrthoDB" id="48057at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000041">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF212">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="161"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="397"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="460"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="399"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="460"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1085408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1118370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="61248" checksum="7A9AB294C51AD5E2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEX1</accession>
    <name>A0A5B0NEX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cleavage polyadenylation factor subunit fip1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FIP1_3</name>
        <name evidence="7" type="ORF">PGT21_027712</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEX1"/>
    <dbReference type="OrthoDB" id="2507772at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF0">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="6">
        <location>
            <begin position="287"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="103"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="210"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="441"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="532"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="690"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="121"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="462"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="532"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="568"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="607"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="692"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="761"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="799"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="839"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="852"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="892"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="956"/>
            <end position="970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1087276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="970" mass="105287" checksum="E35EDEE502585183" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFB4</accession>
    <name>A0A5B0NFB4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006888</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1086648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="445556at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="1"/>
            <end position="37"/>
        </location>
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            <begin position="69"/>
            <end position="178"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="617"/>
            <end position="646"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="1"/>
            <end position="12"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="157"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="717" mass="81296" checksum="B751778E479EA154" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFM4</accession>
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        <name evidence="10" type="primary">FAS1_5</name>
        <name evidence="10" type="ORF">PGT21_029848</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        </lineage>
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    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="76"/>
            <end position="164"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="1128"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid synthase meander beta sheet" evidence="9">
        <location>
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            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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        </location>
    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08354"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16073"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17951"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1087364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1271" mass="141009" checksum="DEB36B0110828695" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGE9</accession>
    <name>A0A5B0NGE9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGE9"/>
    <dbReference type="OrthoDB" id="3255021at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17746" checksum="4AB4BFBEBB72101A" modified="2019-11-13" version="1">MGNEFADRKAAEARNPAFLDTSIRGSLAAEKTALIQREQLWKRSPRVAFPTQSAIHQLCSGHVPLNAFQFKSKKVPYLICTTCGATKSVDHYLVSCSRFNKARTVARRILQDERITEISTRSLLHNPRAFKATKSFIQISARLPQFRQREDAKGSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJS2</accession>
    <name>A0A5B0NJS2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Endoplasmic oxidoreductin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGT21_004415</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May function both as a monomer and a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJS2"/>
    <dbReference type="OrthoDB" id="269384at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF0">
        <property type="entry name" value="ERO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022800853" description="Endoplasmic oxidoreductin-1" evidence="17">
        <location>
            <begin position="29"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="472"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="617"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="492"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="519"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="536"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="619"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1089023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="71828" checksum="B50FF0B9833D71D4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKZ5</accession>
    <name>A0A5B0NKZ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKZ5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023116795" evidence="1">
        <location>
            <begin position="21"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="14937" checksum="C2F3BFCCE8EC943C" modified="2019-11-13" version="1">MQLSNILCAFHLLNCYVILAYPTLHSKTLEKREIEGFSADTQHQHLQKRMEGSSKNPTYGEFLAFVHEVGSTDQEIKSTREKYNSLALQAEIQDTMLEKIDAISERFDANVKFEESDKRLKELQDKARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMR3</accession>
    <name>A0A5B0NMR3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012698</name>
        <name evidence="3" type="ORF">PGTUg99_030820</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="774557at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF12">
        <property type="entry name" value="ANCESTRAL COATOMER ELEMENT 1 SEC16_SEC31 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="617"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="753"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1341"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1417"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1555"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="621"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="765"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="801"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="851"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="873"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="917"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="927"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1426"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1555"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1576"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1599"/>
            <end position="1618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1626" mass="177238" checksum="518E15C287C15C07" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNY8</accession>
    <name>A0A5B0NNY8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNY8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="11797" checksum="28091972E33E1532" modified="2019-11-13" version="1">MAQSVVEEQQEESLHHNLRNILAKLNINTVEEFFGLDKKQIRRWLSLIAIEIVQSIPTSSSDFMLWYATLMATILFLIDKGCFRASCRPHPFQPGQIQPRNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQ27</accession>
    <name>A0A5B0NQ27_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6534 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQ27"/>
    <dbReference type="OrthoDB" id="2496578at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045339">
        <property type="entry name" value="DUF6534"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40465">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40465:SF1">
        <property type="entry name" value="DUF6534 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20152">
        <property type="entry name" value="DUF6534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6534" evidence="2">
        <location>
            <begin position="178"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="242" mass="27430" checksum="5389828293B72993" modified="2019-11-13" version="1">MSVQQGYPKIHDRYDYVEYILPGFVTGVYASACFGGMVISEGIRVLYRFPQDSTIMRVMVLWVMGCSLLHTSWFEYIAYHYAVDGFHDVRKLNAVTPHLGAHVFLSRCLVTTSQLFFCYRLYVFSSRDWRGTSLAILLTLGHVASAIGTMIQFVRAKYYTELFLLLPLGFLYSTTISGDILITGSLLYFLGKQKTHYIKTKSIIKRIMLLSFQTSFLTTALAIVSIGLLGAQRELGILYCLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NRS2</accession>
    <name>A0A5B0NRS2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Eukaryotic elongation factor-2 kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">EEF2K_28</name>
        <name evidence="3" type="ORF">PGT21_031894</name>
        <name evidence="2" type="ORF">PGTUg99_032900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NRS2"/>
    <dbReference type="OrthoDB" id="2500644at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903013">
        <property type="term" value="P:response to differentiation-inducing factor 1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF2">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="427" mass="47591" checksum="162F50C02374313D" modified="2019-11-13" version="1">MGDMLKCLKCNRWSTEKHFDNHCLECIVSHIKTGPSQANQSSPSGPSAPHTQILPSRIHTAGPAYSAAPRRVSTPHDTGTHVFYRGIAKELRGVKKREASATQSSRASTSRTPDPKQSPEKRFVKAGVTVYIDDQPQKKMGIIPKVLEVDTKKPNFYEDFRHHLWTLFSEEILAKTEITFPPDPEQYTCLGTLNSKIENHGTLLDLARTGKTIKSAAVINLFYQHPIEAFPDTVLPTETRTNRKRKYNADPPKTSKSATWALGGKIATKPVRGPAGLSAQVDTLSHPVSQSITMKTDGWVVAQRLHFYTIEEQPPPEANQKSYVINDYIHAVTFPFDIKVDRKKHVGEGSSRQSYPAQVRSSENGQEVINKWVAKIRYSDPQPNVSNHASDAITYRGFGLILAEFKDLISEKLDGLLKIKGSQIEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NT04</accession>
    <name>A0A5B0NT04_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000087">
        <property type="protein sequence ID" value="KAA1091822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NT04"/>
    <dbReference type="OrthoDB" id="2510650at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="593"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="672" mass="74714" checksum="681A7E58ECF46276" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NX38</accession>
    <name>A0A5B0NX38_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024989</name>
        <name evidence="2" type="ORF">PGTUg99_005929</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000152">
        <property type="protein sequence ID" value="KAA1127730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NX38"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1093094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1127730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="249" mass="29257" checksum="6CE57D557CB676AE" modified="2019-11-13" version="1">MEDQKQLVYRILQKVFRNLSQQPIPRQGSVLKPNRQMTSSARFQKILEAFQARDFLADCEHFSPSSSHNDHHDEVDWGRIADARRPFLVLVQDSIAFFQNPVSHLDSEVHDPIRLEFQVVFYLLHFLDAYYHPLLNAILVQRSPSSSSSTILFQNQLGFVSRYLRFYRNYPLNNREHEREKELNLQHDPAAFFFGLATLPAFRSWANSLFKVLQMPSCFDSLLPLHHQRLPDHVHLDTLKLDAWMNKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZ92</accession>
    <name>A0A5B0NZ92_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_025113</name>
        <name evidence="10" type="ORF">PGTUg99_006878</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000152">
        <property type="protein sequence ID" value="KAA1127733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZ92"/>
    <dbReference type="OrthoDB" id="2849579at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969:SF69">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033845134" description="RING-type domain-containing protein" evidence="7">
        <location>
            <begin position="17"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="138"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="34"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="191"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1093098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1127733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="267" mass="28997" checksum="B939CB6E6AED9829" modified="2019-11-13" version="1">MVTWLLLLILVGNSLGGGGEQELSRFADPVQDVEAASQSDGVRRHFSPSTVDLPNGSHTSKNDGVGSLPASTPQTSQPRHEVILQIDDEERPEDKKRAPSDLHTGKGQAASSDERRVYSLGKEKALAQSHQVKPNLDCPICLAELDPRSGPIITSDCGHSFDKYCINCWLYTERERPPYTCPVCRAELRLGGKPAEPPKDPPEGLPLPTTDEVDQAEPPLTPRNRNLLLDNWVCASLLSDATVGHAMAVVLSIYIIVMILGKVLPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0Q1</accession>
    <name>A0A5B0P0Q1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0Q1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="164" mass="18672" checksum="6C011612F7783185" modified="2019-11-13" version="1">MTKIIAENTAKSEKACSKLVVSVDKLNNDLSTTADHIISTVREDDIIENAKRKDELDTVKDLLIQQNIRTEGLSNLLKEQMSLLKKDMKSEIQSSVQASLAQYIFQNDDPNSVNQPKNGTHHQPPRFNNPYMDEKIIQKDRDIAPHLMQMMSLSLADFQGYLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8R0</accession>
    <name>A0A5B0P8R0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005122</name>
        <name evidence="4" type="ORF">PGTUg99_033966</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8R0"/>
    <dbReference type="OrthoDB" id="10325303at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474098" description="Hydrophobin" evidence="2">
        <location>
            <begin position="21"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1134256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14513" checksum="FED700FD85657215" modified="2019-11-13" version="1">MNLSNFFNSLLVVILIQGEALHVQSFDCKKEIYISGYDQAYCAIYGLDKPRYHPPPPQKKPGPTFPDPQPDPQPDPPGTPSHDGYSIVKTTVSNTGDNSCRSGEPTCCGSNVKVNTFARMDFYENNCQPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8U2</accession>
    <name>A0A5B0P8U2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8U2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="14909" checksum="04F79EDB7AF8325C" modified="2019-11-13" version="1">MRFEIFSHTVFQALVIQSFIVAAIVSAAKVRIGETSISPPAAEPQTFETQLSSLLGKSCRACPLGKIDRIIHQPGQTCQHIMKCEHGFPVGPCMGVKPVAQPRCDRCFANIGIIGICEMAEADGTHANVPECPSECLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P943</accession>
    <name>A0A5B0P943_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-P-Glc:Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.267</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ALG6_7</name>
        <name evidence="15" type="synonym">ALG6_6</name>
        <name evidence="14" type="ORF">PGT21_028318</name>
        <name evidence="15" type="ORF">PGTUg99_031346</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.267" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P943"/>
    <dbReference type="OrthoDB" id="5589195at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042281">
        <property type="term" value="F:dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF1">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="317"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="351"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="608"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="646"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="682"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="714"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="784"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="814"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="832"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="864"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="901"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1098097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1116934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="976" mass="110430" checksum="76A05669719633A9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBL7</accession>
    <name>A0A5B0PBL7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035897</name>
        <name evidence="3" type="ORF">PGTUg99_018182</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBL7"/>
    <dbReference type="OrthoDB" id="2500854at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137739" description="CENP-V/GFA domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="14958" checksum="25A9A22A3F9F29F8" modified="2019-11-13" version="1">MPSQLSVARLGLMCTLLLSLLVAVKAPTGHNRVCAICALDCHDLRARNLRWNGNMIQKPCGELHTPVNGCPSIVPVKVYFCGECGSTDWVPDGKCRGQHNGRVPPERIFLKSEPVPGEPPAVVASSVGSNVVWSSAGSSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PDY2</accession>
    <name>A0A5B0PDY2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_021607</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PDY2"/>
    <dbReference type="OrthoDB" id="7832001at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017149">
        <property type="entry name" value="GSH_degradosome_Dug2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF8">
        <property type="entry name" value="DI- AND TRIPEPTIDASE DUG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037237">
        <property type="entry name" value="Peptidase_WD_repeats_DUG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="53"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="303"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="460"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="8">
        <location>
            <begin position="702"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1099817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="985" mass="108112" checksum="4E30BEEF231B4C9C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIQ8</accession>
    <name>A0A5B0PIQ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036094</name>
        <name evidence="3" type="ORF">PGTUg99_021312</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIQ8"/>
    <dbReference type="OrthoDB" id="2511315at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="406" mass="46094" checksum="FA6EDB14B3C35A84" modified="2019-11-13" version="1">MAPVTRASLRRQTPPPRDSHPQPENPKKKPTSSAKRPNLPTAGSSRVKGPQTSNQPPRTAQAPSHPHSHTRVIQTNRTPAHPRASSNHSGSGSGSHRFSHPPTSRILNPHLNAPDPLLVATRSSQLEPHSRSEFDISIPTAPPLSEPGRFSISHADTPRSTNSVSLASSDSDSLPFDELASIIASVPEDLKFFLSPNFSLKRATIKKLTRVIQYFFSGTEFPGNPHKEVFLDFFREHVRPLIDSYCQRTGEQFVETQYHHTTHVKVDHINVSQFDPNLRSSTKAILRALIHKVQPSLRTQYLNKDSLIDLFREVYDLAAVQPAPKYSVCPPPIGIPQHLAQERNDLRFALQVYHPDLFIRLECDKDELLALYELFILEDYRNEHRVREYVHYYWFDPTIPPQFLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PL42</accession>
    <name>A0A5B0PL42_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_017525</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PL42"/>
    <dbReference type="OrthoDB" id="2505764at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000251">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF39">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022903294" description="mannan endo-1,4-beta-mannosidase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="11">
        <location>
            <begin position="146"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="557"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="564"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1101354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="623" mass="69823" checksum="A4FB6938E50C2D49" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLN4</accession>
    <name>A0A5B0PLN4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLN4"/>
    <dbReference type="OrthoDB" id="10271056at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022881681" evidence="1">
        <location>
            <begin position="23"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="155" mass="17201" checksum="0661A131C4A91B2A" modified="2019-11-13" version="1">MKAYTFLIALWFASSMASGTQGFTEEAVRSSDWREMSLIKREPFVQLAPFLAFSDGKLIAPWGSTDATFEHASDNAKVTFQWCSDREAGIVRNLSPCPIAFVLRDVDGRKFVCGFDVPPLLSQVVELPARRQHGWVCLNIKGKQGNIGQTSTAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRN7</accession>
    <name>A0A5B0PRN7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRN7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="281" mass="31460" checksum="2A5AB819AD5122BB" modified="2019-11-13" version="1">MVLLFSFTIQVARSQLLNQKKNNKFLLVFSSIHLLPLSVFSPIHLLPLSVFSPIDLLPLLVFSSIHLLPRSVFSLMVLLFSFTIQVAQSQILNQKKSKIKNSTLSRSSFSLIDLLPRSVLINLLPHSVFSPIDLLFSLLVLISMILFPFNFQGTRSLLPHSVFSSLFRSLDLSFSIKKKKQHPLSVLSISRALNLFSLARSSAHYSGHSISSPSLGLQLTIQVARSQLLNQKKKNSILSRSSFSLINPLPRSVFSPIDLLFSLLVLISMILFPFNFQGTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRW1</accession>
    <name>A0A5B0PRW1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016372</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRW1"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01846">
        <property type="entry name" value="fatty_acyltransferase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF139">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023141913" description="SGNH hydrolase-type esterase domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="33966" checksum="95B50805F5DE3E3E" modified="2019-11-13" version="1">MSWLGFICFLAMCSFVLPKAIALTESLEYSSVVVFGASYCDNGHPRESKFSSSLRQPPYYQGRWSNGPVWVEHIAEVMGIPLYNYAYGGATANNELTNSGVPDTRSQIQYYKNDIESGKIHRGRGRVLHLTWVGINSIHHIWHAGEGVEKAIHEADEVRRQVSSLLSDPTINSQPSEFHVLTLPPLQIVPNDAFAGPSPGWGYGQVSIKMLTEAYNKRLISAINSLNSPSAYVHDIRSFWFRVQRAPDSFGFTHVNAPCLYYSQPCKSPGEYMYFDSLHPTTRMHALIGDWVMSTLSGVSYKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRX8</accession>
    <name>A0A5B0PRX8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024831</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRX8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="112" mass="12703" checksum="8AC68727A093AC73" modified="2019-11-13" version="1">MKPNLTHRWTTGGYAQTPRHFSETASQSSCGSGFQAVSVATCTRRRYTHNFNKIFMLAASEERYPVGRLRTEWRPLFVTGHASTNLKSRFIQTVLPNWLSVNRTIARSNAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PS06</accession>
    <name>A0A5B0PS06_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">S-(hydroxymethyl)glutathione dehydrogenase</fullName>
            <ecNumber evidence="12">1.1.1.284</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">FDH1_2</name>
        <name evidence="15" type="ORF">PGT21_018439</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>S-(hydroxymethyl)glutathione + NAD(+) = S-formylglutathione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-(hydroxymethyl)glutathione + NADP(+) = S-formylglutathione + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>S-nitrosoglutathione + NADH + H(+) = S-(hydroxysulfenamide)glutathione + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:78371"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:145544"/>
            <dbReference type="ChEBI" id="CHEBI:229723"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.284" evidence="12"/>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PS06"/>
    <dbReference type="OrthoDB" id="417550at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106322">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106321">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080007">
        <property type="term" value="F:S-nitrosoglutathione reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08300">
        <property type="entry name" value="alcohol_DH_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000001">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014183">
        <property type="entry name" value="ADH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02818">
        <property type="entry name" value="adh_III_F_hyde"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF12">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE CLASS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="14">
        <location>
            <begin position="34"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="13">
        <location>
            <begin position="205"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1103452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="383" mass="41130" checksum="1C6DF9B0B89F4EBE" modified="2019-11-13" version="1">MDTQGKKIVCRAAIAWAAGQPLKIEEIEVEPPRKDEVRIKVLYTGICHTDYYTLSGSDPEGDFPSILGHEGGGIVESVGEGVNDVKAGDHVIPLYTAECRECKFCTSGKTNLCGRVRETQGKGVMPDGSVRFHCKGQPIKHFMGCSTFSQYTVVSKFSVVKVVDEAPLDKVCLLGCGITTGFGAATKTANVTEGSTVAIFGIGCVGLAVIQGCAARKCKSIVAVDVNPGKEAWAKKMGAHEFVNPTKTENLAQDLTTKYSDGGFDYTFDCTGNVDVMRTALEVCHKGWGVSTIIGVAAAGKEISTRPFQLVTGRKWQGSAFGGVKGRTEIPGLVDDYLKGVLSVDDYVTHHREFAKINEGFQDMKDGSCIRCVVDMWKIANTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVI5</accession>
    <name>A0A5B0PVI5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVI5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="174" mass="18866" checksum="8CC05A5C1A04C4B6" modified="2019-11-13" version="1">MGPPPRYVEALSAPRDGGWVVVTVVQATRSSVNRFQPTSTAAAHHSLPLPASRSHFPPPQTVLLPSPNSLTRSPSLYRSHPHTADRLPAPHSTGSCSAPTKSSYRFCTVFAHKLLPPSACAFSQALIYRPTLIVWEHFQKFKVISPSSTTNHGTQTSRSAVIILKHLSPVSNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVQ4</accession>
    <name>A0A5B0PVQ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005975</name>
        <name evidence="2" type="ORF">PGTUg99_006829</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000315">
        <property type="protein sequence ID" value="KAA1104890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVQ4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="102" mass="10761" checksum="CB83A4F51C5D3DE5" modified="2019-11-13" version="1">MDPATATKTLTGSPSHPRMQLIKGLAQVVMAARVGGVDGGAIATLACFQLEVIQQEKKSGPGLVLHSTLHQNQDRIPSKNTSVSLKPLLLLNSSISFQSQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PY57</accession>
    <name>A0A5B0PY57_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PY57"/>
    <dbReference type="OrthoDB" id="2497685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022934219" evidence="2">
        <location>
            <begin position="26"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="873" mass="99780" checksum="C0CD3DD20C408769" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZ10</accession>
    <name>A0A5B0PZ10_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZ10"/>
    <dbReference type="OrthoDB" id="2495444at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="669" mass="76650" checksum="A4A0290F8A3E62F5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0A9</accession>
    <name>A0A5B0Q0A9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0A9"/>
    <dbReference type="OrthoDB" id="10348496at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1106469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="188" mass="21103" checksum="660F4F815C5BB181" modified="2019-11-13" version="1">MGKTTANQRRHCCNDRHQILVRAEKDRVNRIASWRAENQASGELASVVDEGITQLDVVVDQNRETCVDGFESDPDENELVPEPEIVPLDIFEEEESEIDDRDEDALWMSFINDAVGQISSEPDVLSGTDPLPSFRIEEENIDNVTTDNSAWFPFLNKEVSTLPNIRIFIINSDLSSPLATSNSLDGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2I8</accession>
    <name>A0A5B0Q2I8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011074</name>
        <name evidence="3" type="ORF">PGT21_011587</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2I8"/>
    <dbReference type="OrthoDB" id="2508050at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137938" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12688" checksum="129984FCE5D79DF0" modified="2019-11-13" version="1">MPTIKSIFLLTVACLLSAPMAKGSPRMDPASSGCAHSIRSTASKDFTCQATYICYAGYSHPCGYGLTGEVEQCESCNHYSILSISTCPYGNHPSRSCRKHAVVNPRAGERRKYTDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4A4</accession>
    <name>A0A5B0Q4A4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031698</name>
        <name evidence="4" type="ORF">PGTUg99_013009</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4A4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366491" evidence="2">
        <location>
            <begin position="24"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1107932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="641" mass="75045" checksum="2A6EF70127B73329" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4L7</accession>
    <name>A0A5B0Q4L7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SUMO-conjugating enzyme UBC9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin-conjugating enzyme E2-18 kDa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">UBC9_3</name>
        <name evidence="14" type="synonym">UBC9_1</name>
        <name evidence="16" type="synonym">UBC9_2</name>
        <name evidence="14" type="ORF">PGT21_029751</name>
        <name evidence="16" type="ORF">PGT21_031347</name>
        <name evidence="15" type="ORF">PGTUg99_024793</name>
    </gene>
    <organism evidence="15 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 18">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1108027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4L7"/>
    <dbReference type="OrthoDB" id="6600758at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019789">
        <property type="term" value="F:SUMO transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000035">
        <property type="entry name" value="SUMO-conjugating enzyme ubc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1096833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1108027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1113429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="157" mass="17692" checksum="0A48F8FA9D0FA73B" modified="2019-11-13" version="1">MSAICKARLAEERKQWRRDHPYGFYARPTKNADSSLNMMIWDVGIPGKENTNWAGGLYKLQISFPDDYPSKPPKCKFTPPIFHPNVFPSGTVCLSILNEEKSWKPAITLKQIVLGIQDLMNDPNNADPAQLDAFSLYKSNRAAYDAKIKEQAKSMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5E8</accession>
    <name>A0A5B0Q5E8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006674</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5E8"/>
    <dbReference type="OrthoDB" id="4605274at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053228">
        <property type="entry name" value="Stereospecific_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574:SF1">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="428" mass="46685" checksum="D5BA9A8D977670D2" modified="2019-11-13" version="1">MLLYRMHSVHFKRHNIEYWDYKLSSRRMGFTSWIFVFLLIGPLDCISTSLPLSKRQTLLDIISGNDRFYDVTASPEGPLPPQVPGDAPWTQAESSYQKMISCPLGIQNKERGIVLLVPGTAGSANEAFKSSAYYQVLPSQGFDVCWVDTPNYSLTDMQLAAEFVAYAIKSLAPQSIKTGGKINIVSYSQGGPNVQWASTFWPSIHKLITGHISLSPSMRGTLSSLILCPASNIAGGCLPSVLQQTKGSNYMNAANSLKDTRSAAYALIPTTIIYTLSDEIVTPQLGYSASSRLIGASNIVLQNICPLSFDVDHLGIPGDVGAYGIALDALLNGRPAQPSTVDRSYCTKTAASLGFRLNNAANDLRFVFRTIFGTRRFQMISSELQTLRVPVEPLLQQYVCDRGYASSGCTRNGFRDSAPTESIFAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q617</accession>
    <name>A0A5B0Q617_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ornithine aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_010518</name>
        <name evidence="6" type="ORF">PGT21_011692</name>
        <name evidence="4" type="ORF">PGTUg99_031895</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="4 8">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:13877"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q617"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1083392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1094126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1108404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="87" mass="9231" checksum="AA87D97DAE61E77A" modified="2019-11-13" version="1">MNAGVKAVETALKLAQKWAYVEKGVLDGKAIILSVGENFHGQTLGVMGMSTDPDYQNSFGPFLTSIGSSCPINEPANSGQSDFHDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q718</accession>
    <name>A0A5B0Q718_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030780</name>
        <name evidence="3" type="ORF">PGTUg99_037760</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q718"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="116" mass="12695" checksum="9C602AAA4A95B884" modified="2019-11-13" version="1">MQVTSSATGSDSHQYWQPARTGISDYSLTPAPALLLSKSFDGMYRNLTPVSNNRASPSPRRMKLPRGLDPGWDIAVDRTLRTPHSTSTNPKLDTGHQLSLASRITHHIPSSSKLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q776</accession>
    <name>A0A5B0Q776_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q776"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="108" mass="12488" checksum="3F881F03A2DDB5FD" modified="2019-11-13" version="1">MKSKHPPHPGRQTTTHRMISSPMADYQFEKLIPASSLCPKAPQIGRSKCEPINYLLEPGDKKTNPNHDFPRHHYNHDHLRQAHDLLPDKENNPTLAIKLSYPWFSNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHU3</accession>
    <name>A0A5B0QHU3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHU3"/>
    <dbReference type="OrthoDB" id="10269039at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023105771" evidence="1">
        <location>
            <begin position="24"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="132" mass="15307" checksum="6817D92B2D23902D" modified="2019-11-13" version="1">MKRSPIWILALLLMLEAICKTSAAGEVHEITSWVSKENNAIDWSTMKFLRDFQPSHDIEDHYVCHVGCLHLSLSRVAGSKIKLVNKGDTSFQYAVQDMLTKRWIWEETIAPDDVHIFATTHVQFYLINHPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIP1</accession>
    <name>A0A5B0QIP1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Tubulin gamma chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TUB4_3</name>
        <name evidence="13" type="ORF">PGT21_017067</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIP1"/>
    <dbReference type="OrthoDB" id="10249382at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02188">
        <property type="entry name" value="gamma_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000004">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000003">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000045">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002454">
        <property type="entry name" value="Gamma_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01164">
        <property type="entry name" value="GAMMATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="11">
        <location>
            <begin position="66"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="12">
        <location>
            <begin position="267"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1112965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="483" mass="54127" checksum="49F2F4B86246BA42" modified="2019-11-13" version="1">MPRELITISAGQAGNQISSEFWPQICAEHGISPTGLLQPPPQPSSSSGTTNNNPFQTNDCSAIDRKDVFFYQADDDHYIPRAIMIDLEPRVINTILTSEYSKLYNPENVYLSKDGGGAGNNWAAGYETGEKIYEEVMDMLDREADGSDSLEGFMLLHSIAGGTGSGLGSYLLERLSDSYPKKLVQTYSVFPHSDDSSDVVVQPYNSILAIKRLVNHSDSVVVLDNAALARIVSDKLHVSSPTWQQTNRLVSTVMSAATTTLRYPGYMNNDLVGMIASLIPTPRCHFLMTSYTPFTSERDGQEEAKSVRKTTVLDVMRRLLQPKNRMVSTGATINKSACYISILNIIQGEVDPTDVHKSLLRIRERHLANFIPWGPASIQVALTKKSPYIQTQHRVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLDQYKKTNMFANSWDEFDESREVLVDLIDEYRAAESKDYVNYTAPKSSLEDDRTHQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIT6</accession>
    <name>A0A5B0QIT6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_018095</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIT6"/>
    <dbReference type="OrthoDB" id="17687at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000051">
        <property type="entry name" value="Probable MDR1-Mac1p interacting protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000071">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="228"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="628"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="664"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="729"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="915"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="729"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="752"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="918"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1025"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1035"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1093"/>
            <end position="1114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1112999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1122" mass="125262" checksum="C2404E2EED10EF8F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJB8</accession>
    <name>A0A5B0QJB8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJB8"/>
    <dbReference type="OrthoDB" id="2496748at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48159:SF1">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GIANT PROTEIN ANTIGEN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48159">
        <property type="entry name" value="MULE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="472" mass="53017" checksum="0B55ED13100AD9EC" modified="2019-11-13" version="1">MSFVQTIILPTSNAPANMYPLVPNIEDIVPEPFTQEVPLPHGIKAPPSAMRMVRWLGGAAPSFDNNPHCFSIPGKSLGDAQAFVESMQATFRWSLQNFFDNIPTSPPVKKLGRPPEYHFKLYYTCPRRGHHLPRINSRKEESGRKCGCEAKFNIFHHIATDSLRVEWHWQHSHDLNTHEDMKHTRIPKAVHDWIVDRVDSGIGWKGIQNLLSSPDLEAARRDPDVFVSLSLWQAHLNHNGLHTFAPVLSDSDTFLFAIQSPWHWQRCNRSDPRIGIYRGSDRNVPQYRVSGSDRFSDPIPIYQMIGISWGDPIRAAWELGIGIAQADPRIPRSCIVARPHYLGPEIARPPPLASGRTRNRSDTARPPPLVVPGRCPPLRRWVLNLATPHPRRFTLQTSGRTRTRTDVLGLERTPPGLHHSSFPGCPPICRCFAGRAAAAAAVVALGVVGSTWYPPPTTFICFSRCARQLVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMR3</accession>
    <name>A0A5B0QMR3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008855</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMR3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="10971" checksum="DEBA48E3DF83CA5A" modified="2019-11-13" version="1">MGIGRDELMISQRGADLPCCRSGLQLIPADSDIVNLEECHMDETGRFLQLGAGSHSSLTANDGFFNTNAPCAPTGMLAERRASTLVSVNPPGFVKLQIPTEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNH9</accession>
    <name>A0A5B0QNH9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNH9"/>
    <dbReference type="OrthoDB" id="2497097at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="257" mass="30018" checksum="E572B7FBE29A2F0F" modified="2019-11-13" version="1">MQPSFRARFDFIFSLPDGYTRFQAFLDRGDGALFPGHLSYDVFKKVSEKFEQEFRTKIHRTNLRTDHAALQIALLSEKTSKDVQVMRVLDSTMDRPYTIEPFISVYNHLIKSVEFLHVAILGQLELTRGERNRRRLDVLYWIGEEITKPKESLPVFGMIQDNIDSSNMEDRMLSGEKLFGPIQVSLIKYFSSSELARSGHFPETRFTAAFALASWYEVNHPEDFNNFFFHQSSRCESLEEALELKKSYIHPFYCRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPC1</accession>
    <name>A0A5B0QPC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Eukaryotic elongation factor-2 kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">EEF2K_21</name>
        <name evidence="2" type="ORF">PGT21_028267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPC1"/>
    <dbReference type="OrthoDB" id="2500644at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903013">
        <property type="term" value="P:response to differentiation-inducing factor 1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF2">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="427" mass="47508" checksum="5D405A418F4EC35E" modified="2019-11-13" version="1">MGDMLKCLKCNRWSTEKHFDNHCLECIVSHIKTGPSQANQSSPSGPSAPHTQILPSRIHTAGPAYSAAPRRVSTPHDTGTHVFYRGIAKELRGVKKREASATKSSRASTSRTPDPKQSPEKRFVKAGVTVYIDDQPQKKTGIIPKVLEVDTKKPNFYEDFRHHLWTLFSEEILAKTEITFPPDPEQYTCLGTLNSKIENHGTLLDLARTGKTIKSAAVINLFYQHPIEAFPDTVLPTETRTNRKRKYNADPPKTSKSATWALGGKIATKPVRGPAGLSAQVDTLSHPVSQSITMKTDGWVVAQRLHFYTIEEQPPPEANQKSYVINDYIHAVTFPFDIKVDRKKHVGEGSSCQSYPAQVRSSENGQEVINKWVAKIRYSDPQPNVSNHASDAITYRGFGLILAEFKDLISEKLDGLLKIKGSQIEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPV3</accession>
    <name>A0A5B0QPV3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPV3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="67" mass="7596" checksum="8C8A24E79B5C4742" modified="2019-11-13" version="1">MNIGPLLHFDGCNPEGNPDPKATSGVNAWGYSRRNGYIRVQDLTDNYWTCPYNAHNQIEYITCDTCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXA4</accession>
    <name>A0A5B0QXA4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXA4"/>
    <dbReference type="OrthoDB" id="415023at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22748">
        <property type="entry name" value="OTU_OTUD6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000025">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="242"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="410" mass="45572" checksum="0F4B4FE50834D1CF" modified="2019-11-13" version="1">MTNSNQAEHQQQQQQTNGAGEEKQQENGTTTAKTIKTAADILSRPRLPPKKKLSTSNKKPGKKNKNKNEQLVTATEKLEEKEEETEGHPTESIDNDQLLDQLLTQTNHLTLSSSEPTTPSTPTTTKTQQQQQQQQQQQQQQEANGNPSRSSTISRKLHGLSEDIKEVAHAVSSSINPSSPGKLASIKPKKKPHRTKEKIAKRQAAQLQAQLDAQLESSIHPVIDHRQLEAEALLRICLSLKLQIFEIIPDGHCLFSAIADQMNVLSGQTNGPSTATTTPTKGDEAVGGALRYTYQTCRKLASEYMRKHPEEFIHYLPGQDDGLEAGLMSSKEYEEHCDRVRDSACWGGEPEILALSKALKFSIHVIQAFHPTIKVSEEFLEPRGNIALTISYHRKSYGLGEHYNSLRPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXD5</accession>
    <name>A0A5B0QXD5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXD5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022683582" evidence="1">
        <location>
            <begin position="24"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="16566" checksum="0BBBD7703D95BE8B" modified="2019-11-13" version="1">MRWFSHSFTFLAMFHIYLPPTLHQSMKDPADIIKDVLGRRCHDCNRKYYIHRIEPQEMVCDARLSCRHGPYTGTCVGKKTVYIIKCNRFRCRSTRKIWHGCTKSKANDNYIYTHPLTECNLDIKLDSKIQLFKHEHTNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXR1</accession>
    <name>A0A5B0QXR1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Alpha-galactosidase</fullName>
            <ecNumber evidence="3 7">3.2.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Melibiase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_031866</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="3 7"/>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXR1"/>
    <dbReference type="OrthoDB" id="2497659at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF75">
        <property type="entry name" value="ALPHA-GALACTOSIDASE MEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00740">
        <property type="entry name" value="GLHYDRLASE27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha galactosidase C-terminal" evidence="9">
        <location>
            <begin position="348"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1118117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="571" mass="64047" checksum="7492FFA2A01C167A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZN9</accession>
    <name>A0A5B0QZN9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025573</name>
        <name evidence="4" type="ORF">PGTUg99_050066</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000247">
        <property type="protein sequence ID" value="KAA1118559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZN9"/>
    <dbReference type="OrthoDB" id="3230070at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09276">
        <property type="entry name" value="Rnase_HI_RT_non_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33481:SF1">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33481">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="362"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1118559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="77143" checksum="855C71F6909D8037" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R116</accession>
    <name>A0A5B0R116_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004284</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R116"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1118745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="107" mass="12125" checksum="CC2E45AF7D8637C8" modified="2019-11-13" version="1">MVSSKAQEIYASARSTFYWSNQLLEQQYWATKLFDQNMLQAAMSERVAGTSTCACNWRKVVALYIDTNRSATLTRLFTVVTWITRGAQTGGPDDACFWERLEPSGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1C1</accession>
    <name>A0A5B0R1C1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">acylaminoacyl-peptidase</fullName>
            <ecNumber evidence="5">3.4.19.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Dipeptidyl-peptidase V</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_015186</name>
        <name evidence="12" type="ORF">PGTUg99_037781</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S9C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.1" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1C1"/>
    <dbReference type="OrthoDB" id="43744at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000903">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045550">
        <property type="entry name" value="AARE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF4">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19283">
        <property type="entry name" value="APEH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylamino-acid-releasing enzyme N-terminal" evidence="10">
        <location>
            <begin position="26"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="9">
        <location>
            <begin position="539"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="9">
        <location>
            <begin position="691"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1119103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1122550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="769" mass="85560" checksum="AD02BCC9901B98CB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4H0</accession>
    <name>A0A5B0R4H0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_037240</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4H0"/>
    <dbReference type="OrthoDB" id="3437960at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000201">
        <property type="entry name" value="C2H2 finger domain protein (Gli3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000031">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="212"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="243"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="422"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="8"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="435"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="461"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1120183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="500" mass="57201" checksum="9C32764A752A9A71" modified="2019-11-13" version="1">MEPEKNYRNNNNNNSTQPTTTTTTQQQPRSTGGIKRTKQTNTTSSSAKKKRTFDSISNSLPAEESIEAQTIPPNYNNNQQQQQQQHHHHHHRNPQSHQQQLILNQFNHHHHHVQPQTQPGPNPQFQLSIMPTDEDFPMIRCGWNNCRQGFWILEDLINHLVGENGHVPPDPTAPRGQKCPCEWVGCPKQGKPQGSRMALLVHLRSHTGEKPFSCHKPECDKTFSRTDALAKHVRVSHGEILPTVRSSTTSQLKTSSNSNTNGGKKIKAENESEGEEEGTVVAEELGIQGKSIDCSTGSDLPQLVPPPPGGPSAEKDLLEIINENGKWFNETIDNELRTEEERKSLEELRFKYPSVDFAFLEFVVLKAKIKFALGEREILKSELNLVQLKEENIKKQKDSYLDEIMRQQIGPESSELWRTTIDNISTLPPPPSTALHHHHHHHHHHHSHSHSHDQHLHEKQQLTQQQQLAHQQQLAAQQQQQQQQQPYHHHHHLHLQQLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R6D9</accession>
    <name>A0A5B0R6D9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025457</name>
        <name evidence="2" type="ORF">PGTUg99_021783</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1120948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R6D9"/>
    <dbReference type="OrthoDB" id="2506921at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="408" mass="46374" checksum="3D51CC7FCA11F00E" modified="2019-11-13" version="1">MDPNNTPGEKEHWPQGDLVVQRFQSLINKCEPINNGDPYTGSRTIRKAFPIEQVNMQNDLLTELRSDHLPQLGRQITDLVPALDPARLKEDPGLQLQIVLGIQAELEQTLHQIQSALSILCPGPLPSEIIRTNDQHLKGLKSFRREGLHYRIIEEMLPDVICIFHQSETLILLMKLSTKKAPRHPGIALTRENIIEYESSALEGIRMGIQWLEGSEFDLVQYHWPNEIRGMDRDLEELSEVINGTAHLKKGSRMPGPLSDPAVQLSRSLLPILKLSRLFFNKLSNRAMNRKRLSLHSEMRSDQLDTLYELAGNVNCELSEVLNLLRTADRSDGRSARPACTRIVTALEAHFESALLLLHLYFLPLIPDTDGFPIQTYFKAWFATWHTQFGLVIQNFIDTAGLFDEDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RBB9</accession>
    <name>A0A5B0RBB9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035955</name>
        <name evidence="3" type="ORF">PGTUg99_008507</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000212">
        <property type="protein sequence ID" value="KAA1123026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RBB9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="97" mass="10642" checksum="00463D1EF1BEAA89" modified="2019-11-13" version="1">MSPPAARSRIGIEFTSSPLCFLNLTSFIRRGDAVPQGPSESDETAGGTYPKVMSSFPSRARRELSGRLSSHKVATLPSSAHRRIRSLYLHKKVCNPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RIF8</accession>
    <name>A0A5B0RIF8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021424</name>
        <name evidence="3" type="ORF">PGTUg99_015202</name>
        <name evidence="2" type="ORF">PGTUg99_017726</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000175">
        <property type="protein sequence ID" value="KAA1125771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000007">
        <property type="protein sequence ID" value="KAA1137539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RIF8"/>
    <dbReference type="OrthoDB" id="2495932at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1125771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="292" mass="32974" checksum="01559EA8AD8EED7A" modified="2019-11-13" version="1">MTSLPTSNRPDSSEMTGVTQTTYHFNPTMEHNADTRVSIITNAHPSDSWVSAKKENYEGRHDVTRNTLSQPSTESPRTVHVMRLLSDTERSLSGFCYSSPTLPLWRFCSMAGKGLSSKRVEDVKLNRIDKLRAGRKRRANRRPFVFKSSPLAEGQGAGRLTQFHANTFAHPSPSSQSSPFVDRKNTEGMQLRQTTFYNGCNAVRISRFSGPSDPTIKQWRDPSQLERPVTFAPRDCNFNSFPSWSKQPQISRLCSPKSTKPQSSLVQTKTDYQSWEISLPKCPFPNMGMFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RN83</accession>
    <name>A0A5B0RN83_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="16">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PGT21_036278</name>
        <name evidence="20" type="ORF">PGTUg99_017239</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">21-0</strain>
            <strain evidence="20 22">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Protein phosphatase that specifically binds to and dephosphorylates the molecular chaperone Hsp90. Dephosphorylation positively regulates the Hsp90 chaperone machinery.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="16"/>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RN83"/>
    <dbReference type="OrthoDB" id="445564at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000595">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000039">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013235">
        <property type="entry name" value="PPP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08321">
        <property type="entry name" value="PPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033096">
        <property type="entry name" value="PPPtase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="18">
        <location>
            <begin position="358"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR033096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1106573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1126233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="583" mass="64518" checksum="FCF6979401358129" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RWK8</accession>
    <name>A0A5B0RWK8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018364</name>
        <name evidence="1" type="ORF">PGT21_018481</name>
        <name evidence="3" type="ORF">PGTUg99_019256</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000110">
        <property type="protein sequence ID" value="KAA1130346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RWK8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="51" mass="5746" checksum="E576822251476D76" modified="2019-11-13" version="1">MRDFLKWDPDRVEFVVPVSGVFLRDILAFKSALQVLSVDVHNVNPCSTNIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1I0</accession>
    <name>A0A5B0S1I0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019452</name>
        <name evidence="3" type="ORF">PGTUg99_033222</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1I0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138222" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="55" mass="6097" checksum="69A9020B206A5682" modified="2019-11-13" version="1">MFLTTFFQKAHIGLAFLPIMIILHFGQLAPVDGTEVNCLPHFDSEYGNPTACKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S3A4</accession>
    <name>A0A5B0S3A4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_001429</name>
        <name evidence="3" type="ORF">PGT21_001491</name>
        <name evidence="5" type="ORF">PGTUg99_006863</name>
        <name evidence="6" type="ORF">PGTUg99_029299</name>
    </gene>
    <organism evidence="5 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000077">
        <property type="protein sequence ID" value="KAA1132651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S3A4"/>
    <dbReference type="OrthoDB" id="2504110at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1076290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1132651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1135660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="157" mass="16738" checksum="3321B80C864BCC14" modified="2019-11-13" version="1">MTISFSVTPKARIAPSLPSTRILIYTFLFTQLCTSVRSSSCYYDSFTGRTVCDGLSYGARLAIAAAITVVGALVLTGISYVVRKRFYGARTPPPVSAGYYGPPQTSYPPNVAYAQQPNLYPGAPPPAPAYAPPPGPPPPGYNPDFYKQGPPPVPQHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SD24</accession>
    <name>A0A5B0SD24_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine/threonine-protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CBK1_1</name>
        <name evidence="2" type="ORF">PGT21_013564</name>
        <name evidence="3" type="ORF">PGTUg99_014202</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000037">
        <property type="protein sequence ID" value="KAA1135720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SD24"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366694" evidence="1">
        <location>
            <begin position="21"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="77" mass="8638" checksum="873F3848E7FB8825" modified="2019-11-13" version="1">MQQFFEGLTGLFLTCSGALADRTSRKNSEKDGGYQDYVYFSGNPSQFSKPILEKTTACKLKIEHVSRKAVEINVDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LN66</accession>
    <name>A0A5B0LN66_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dynamin GTPase</fullName>
            <ecNumber evidence="3">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">MGM1_3</name>
        <name evidence="21" type="ORF">PGT21_017342</name>
        <name evidence="22" type="ORF">PGTUg99_006794</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 23 24" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">21-0</strain>
            <strain evidence="22 24">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000108">
        <property type="protein sequence ID" value="KAA1130486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LN66"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000741">
        <property type="entry name" value="Putative mitochondrial dynamin GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056495">
        <property type="entry name" value="LIS_MGM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF212">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24550">
        <property type="entry name" value="LIS_MGM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13 17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="20">
        <location>
            <begin position="259"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="19">
        <location>
            <begin position="883"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="851"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="977"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="852"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="869"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="980"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1007"/>
            <end position="1022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1065982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAA1130486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1022" mass="113812" checksum="5A725F3FF6159A59" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPD1</accession>
    <name>A0A5B0LPD1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Leucine-zipper-like transcriptional regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LZTR1_7</name>
        <name evidence="2" type="ORF">PGT21_006941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPD1"/>
    <dbReference type="OrthoDB" id="2505591at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="206" mass="23196" checksum="EE1E81C57736C830" modified="2019-11-13" version="1">MMLLANYNPQDEAASIKEASDFISIQLQHLAPGVFEKYRETLIENNLPSMSHMEYPLPYDALDFASFLTFTMFDFHNGPHCDTDANHWTLVCWIPIFNPRNCSEDDPILADDDFDMLGGQFTFRDFQVCLDLNHVLGVALCVFRSRDYTHQTLPGASPSGKYTRIGFSCQMSEAMSNAVVAHINGTAPFLEVAGQEKQVSNTQKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPY9</accession>
    <name>A0A5B0LPY9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014699</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPY9"/>
    <dbReference type="OrthoDB" id="2150628at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051667">
        <property type="entry name" value="Archaeal_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096">
        <property type="entry name" value="YALI0E33429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="30"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="390" mass="44549" checksum="CD9C3215AA439CFB" modified="2019-11-13" version="1">MHSILTIPAPPTRRVKSGPEERLSRRLKSDPQLTLILGPPSSGKTALARHVASQTRPDGTPEFHPLTVNLRAVDRFHDGSFLRAFVRNGIRAAEIDPFWERPFRGFDDLARVLYSNPPQDVPSSLMAAEVFNELAHRLQPLSYYTRESVRAPVLIIDEANHFRKMSDKAIMTFLDFAVRMTTKEKKMHIIMTSSDSFFLEWIGRCINPTYLDWFVLGDMTRDEAHRYFLHALETDCRLSEEKKAMLGSVDSDTIYRLTGGRPIFIESYIRQVHQSGFFVDPLRFQPVRQAYGCMFNSLGDEPKTYGKAETLAVSSLLVTSPGHHTSYGNLALKLGLPVVEEMFERNFLQYRPPSTFSRDLDPSPHETVVTAQSQPCLRAMEWFVNSHRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQ44</accession>
    <name>A0A5B0LQ44_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Halotolerance protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SAT4_1</name>
        <name evidence="13" type="ORF">PGT21_033532</name>
        <name evidence="14" type="ORF">PGTUg99_017569</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQ44"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    <sequence length="963" mass="104985" checksum="E5ECA44335E66202" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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                <person name="Miller M.E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">21-0</strain>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXF2</accession>
    <name>A0A5B0LXF2_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="2 3">
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1069172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="538" mass="63263" checksum="C05AA84C3F54EA83" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXQ9</accession>
    <name>A0A5B0LXQ9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">NEO1_2</name>
        <name evidence="20" type="ORF">PGT21_017233</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXQ9"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000097">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000254">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="537"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1076"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1154"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1180"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1208"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1234"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="18">
        <location>
            <begin position="147"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="19">
        <location>
            <begin position="1042"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="426"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="13">
        <location>
            <position position="625"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="625"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="625"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="626"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="627"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="627"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="729"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="771"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="795"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="824"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="907"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="908"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="909"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="989"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="995"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1015"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1018"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1019"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1019"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1069235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1278" mass="143501" checksum="F93D8899FFA1AE54" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYD8</accession>
    <name>A0A5B0LYD8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">USP7_20</name>
        <name evidence="11" type="ORF">PGT21_024008</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYD8"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000128">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000215">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 7, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024729">
        <property type="entry name" value="USP7_ICP0-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF644">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12436">
        <property type="entry name" value="USP7_ICP0_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MATH" evidence="9">
        <location>
            <begin position="29"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="188"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1168"/>
            <end position="1193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1069421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1193" mass="135778" checksum="87FB49B6969E2320" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZH2</accession>
    <name>A0A5B0LZH2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7">N-terminal acetyltransferase A complex catalytic subunit ard1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ARD1_2</name>
        <name evidence="6" type="synonym">ARD1_1</name>
        <name evidence="7" type="synonym">ARD1_4</name>
        <name evidence="6" type="ORF">PGT21_006181</name>
        <name evidence="5" type="ORF">PGT21_006670</name>
        <name evidence="7" type="ORF">PGTUg99_015802</name>
    </gene>
    <organism evidence="5 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000107">
        <property type="protein sequence ID" value="KAA1130637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZH2"/>
    <dbReference type="OrthoDB" id="25586at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990190">
        <property type="term" value="F:protein-N-terminal-glutamate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000037">
        <property type="entry name" value="N-alpha-acetyltransferase daf-31-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1070277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1086635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1130637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="151" mass="17425" checksum="9DE36AE2A22748DC" modified="2019-11-13" version="1">MDIRQATVADLPGMQHCNLMNLPENYQMKYYMYHLLTWPQLSFVGVDQKGRVVGYILAKMEEDPADEPHGHVTSISVLRTYRRLGLANKLMQQAQKAMRDVFGARYVSLHVRKTNRAALSLYQDTLGFAVKEIEKKYYADGEDAYSMRMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0E2</accession>
    <name>A0A5B0M0E2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0E2"/>
    <dbReference type="OrthoDB" id="10346499at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="132" mass="14977" checksum="F34CB9C753D288D6" modified="2019-11-13" version="1">MCVMITFRDVPTIQDPLVVTIHLACRQIFRDGAFWKTIEPIFPDSSMHGGPLGENDGSFRATLDIRAPTDGRQLGRPAAVQLGGEARPRMNLERQQRGFVLRERKLFTLLAIGWVILYLIVIELALLARTWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2S6</accession>
    <name>A0A5B0M2S6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent DNA helicase sgs1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SGS1_65</name>
        <name evidence="3" type="synonym">SGS1_83</name>
        <name evidence="2" type="ORF">PGT21_021058</name>
        <name evidence="3" type="ORF">PGTUg99_019391</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000175">
        <property type="protein sequence ID" value="KAA1125779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2S6"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="167" mass="18327" checksum="A0DA78CA2BEFCBEF" modified="2019-11-13" version="1">MARNSKRSGLRSASIRNKKDASSRSVTGIQLSAKATKTHQESRPTRVRLLKKMLEKSDDSLGNAINEQAIKKYSAPAKTLQIRTVVNLARGRNVFLLAGTGYGKSRIPELYYNLIPKETNAVVLVLNPLDALGDNQVLEKTRAGFTTINLTKKTFNPEEAEKIAAGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3H4</accession>
    <name>A0A5B0M3H4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004486</name>
        <name evidence="3" type="ORF">PGTUg99_020644</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000211">
        <property type="protein sequence ID" value="KAA1123184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3H4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="105" mass="12137" checksum="237861A71D9EEC1E" modified="2019-11-13" version="1">MHQITKQTIQSLPYWLLILSSLLAGLVIGDLTCKCRETLKSRHTQMEYMCGSMSRKNNCSTNIVFRQYACSNKDCPLVAMVPDEKHCCNGHDVEIYYYEPSKPKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6I0</accession>
    <name>A0A5B0M6I0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6I0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="157" mass="17065" checksum="85DA9B5A8555296C" modified="2019-11-13" version="1">MKLDPQRCTIMLAVGTVVPSNRSDRTNILVCAGAGCSVSFRESLHPLKLPEFVLGMLEQEIKTLVHFSILLDLLHGTTGTTAINVNLPASMKSRGIEDESLRLRLDNKGESISVSHPLSQVTLKVDSSNVGQTKNQIEKLNDRLKAVEITAEGGETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAI0</accession>
    <name>A0A5B0MAI0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033580</name>
        <name evidence="1" type="ORF">PGTUg99_006481</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1072948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAI0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="94" mass="10836" checksum="F4DB3BBEE8A3C868" modified="2019-11-13" version="1">MTALLNMQLGHAIRCQHPLDRRGWGPLTEIECNQVNHCDWGEHNRCRIPRTGRYQICIICGATFQHHYFVIAYCNSDHRISEDCDGTVTTGHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MD15</accession>
    <name>A0A5B0MD15_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MD15"/>
    <dbReference type="OrthoDB" id="2515405at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="238" mass="27827" checksum="702343F6A3617016" modified="2019-11-13" version="1">MTKPSKSTEDKAYRGLPYHDEWSQSHSAWTNNHRSFYITLRDVYNKKRFAEKLHIHKENCDSIADVYGFMTAFRYNMQIRLNAFAHRVPTKDGAAVPDISVKQMVVVEQCYSTVRSLGEASWKDNNYAPGLSHAGFNPDTGLKQPELHKSASYHANSSRDAPHTQHRMNHQERRQEKRWGGGYQHENYQNYPANFNNNYPNNNFGGHNYGYNNQNYNSRNQFGNRSPYDYQCQLWSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEC0</accession>
    <name>A0A5B0MEC0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026209</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEC0"/>
    <dbReference type="OrthoDB" id="21470at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053027">
        <property type="entry name" value="AGGF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23106">
        <property type="entry name" value="ANGIOGENIC FACTOR WITH G PATCH AND FHA DOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23106:SF24">
        <property type="entry name" value="ANGIOGENIC FACTOR WITH G PATCH AND FHA DOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="190"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="515" mass="58089" checksum="48E39FCA7F4C7F6E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFI2</accession>
    <name>A0A5B0MFI2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="4">5.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">IDI1_1</name>
        <name evidence="14" type="ORF">PGT21_024114</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:23285"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFI2"/>
    <dbReference type="OrthoDB" id="510307at2759"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000012">
        <property type="entry name" value="Isopentenyl-diphosphate Delta-isomerase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018427">
        <property type="entry name" value="Isopntndiph_ism"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="13">
        <location>
            <begin position="79"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1074888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="267" mass="30556" checksum="71E25B46B337F40E" modified="2019-11-13" version="1">MTDELEGHDQEQIALMEERLILLDQDDNAIGEESKKTCKSSLFILLSTQIRNDKVLILNMGLISHSGHLMTNILPPRSLLHRAFSCFLFRPSDGKLLLQKRAAEKITFPSLWTNTCCSHPLAKEDEMDLTDHIGVRRAAQRKLNHELGIDAAQIPVSDFVYLTRIHYLAPSDGLWGEHEIDYILFLTADVDLNVNPNECSDVTWVSPNELKTMIADPSNHFTPWFKLIVDRFLFPWWNDLLDRSKDKLVNAHLIADAKDNTIHRMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFI9</accession>
    <name>A0A5B0MFI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000155">
        <property type="protein sequence ID" value="KAA1075705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFI9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="591"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="842"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="842"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="876" mass="96376" checksum="250D3F28C9EAE54C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MH85</accession>
    <name>A0A5B0MH85_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-1,4-glucan branching enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GLC3_11</name>
        <name evidence="3" type="ORF">PGT21_034139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MH85"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 13 N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="204" mass="22763" checksum="9468E63F083ACF35" modified="2019-11-13" version="1">MGSRRSNASLIGDFNNWDREANPMKKDQFGVLGMSGTTQRLPPGIAHGSKVKISMIAQSGERIERLPAWIRRVTQDLSVSPVYDAIYWDPPQKYQWKNKSPPPLDAVKVYEAHVGISTSEGRVGTYKEFTANVLPRIKALGYNVVQMMAVMEHAYYASFGYQITNFFCASSRYVDVAHGMGLTVLLDVVHSHACKNVLDGNQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJX0</accession>
    <name>A0A5B0MJX0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sphingolipid C(9)-methyltransferase</fullName>
            <ecNumber evidence="14">2.1.1.317</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_010466</name>
        <name evidence="17" type="ORF">PGTUg99_014217</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="17 19">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CFA/CMAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.317" evidence="14"/>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000348">
        <property type="protein sequence ID" value="KAA1098668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJX0"/>
    <dbReference type="OrthoDB" id="412182at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052290">
        <property type="entry name" value="Sphingo_C9-MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197:SF1">
        <property type="entry name" value="SPHINGOLIPID C9-METHYLTRANSFERASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197">
        <property type="entry name" value="SYNTHASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G04190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1076533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1098668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="506" mass="57751" checksum="D9D5F6D3FA2F8F8E" modified="2019-11-13" version="1">MSKEPSKSCVVRLTNYPAIRNAPLIVEGNGRFSNKEMIAAVIFVPYLAGKFLIPGITIRFLSYGHIFLAIILAPAVIISYWALNSIYASQVTNQVALPNNKQSSYFIFKDPKLAQAYNPDNPGSKRIPMQIWHDAFFEGKIEIKRDMMELLEYRHDWAAFHMTPELFKYVVTRLIPEVILHTTSQDEEQVRGHYDRGDDFYSWFLGPRMIYTSGIISDPDREETLEELQDNKLKLVCQKLDLQPEDNLLDIGCGWGTLAAFAAKNYGCKATGVTLGKNQTKFGNDRLAANGIPSTQAKIVCQDAREIPAEPKGRYTKISCLEMAEHVGIRRYPDFLKQVYDLLADDGTMVFQVAGIRPNWQYEDLIWGLFMNKYIFPGADASLSLGWVITRLEQAGFEVKSVDVLGVHYSATLYRWYKNWKSNEAKVKAKYGDKWYRIWEFFLAYSVITSRQGSVSLFQITVHKNLNTFHRVEGVKTHTSLDTLKEPSIVQRRSFEEVICQPVPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLN3</accession>
    <name>A0A5B0MLN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLN3"/>
    <dbReference type="OrthoDB" id="10609747at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="123" mass="13821" checksum="768A98523835A920" modified="2019-11-13" version="1">MLPLATEDDSIDVPAYDDDQASEPLWPTEVSCEMNIEAWQSALLNAGLLPEFEDVISGFNNGFPQAWEVKDKFEERLQKELAAGRMFGPFTHDQVQRCFPFFRSNPLGVVVNSNGSLRPTNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMD8</accession>
    <name>A0A5B0MMD8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_027981</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMD8"/>
    <dbReference type="OrthoDB" id="10276419at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023003278" evidence="3">
        <location>
            <begin position="21"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1078065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="257" mass="28342" checksum="229B3091A3080A8D" modified="2019-11-13" version="1">MAHWLILLAVFGHCTLKVRCGEELSALHLEEADQGADLGSHLGQRPPSDSVPANPANQPIPLQPDADALNHALPASGPHSNQVQQEANIVLPSTDNFLEDSTAQLHASQEDSRAMTALEADPAADQVQAANNHHFVIEQDGTRQSLTERVRTLGRELSRFGVDFGRTLWRNPPPQTLLGAAFAYIMCHPETSGRTLTNLGKQSARSRNGMGTNINSLQLTISDVCSQDSPVVVVLLHILFLVWLYYFIKDFNRYRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP15</accession>
    <name>A0A5B0MP15_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_010668</name>
        <name evidence="2" type="ORF">PGT21_018076</name>
        <name evidence="3" type="ORF">PGT21_019034</name>
        <name evidence="7" type="ORF">PGTUg99_020204</name>
        <name evidence="4" type="ORF">PGTUg99_028035</name>
        <name evidence="6" type="ORF">PGTUg99_035962</name>
    </gene>
    <organism evidence="3 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000337">
        <property type="protein sequence ID" value="KAA1102647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000174">
        <property type="protein sequence ID" value="KAA1125917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MP15"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1102647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1107340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1124850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1125917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="85" mass="9527" checksum="C2164EA4694FFDD6" modified="2019-11-13" version="1">MFKVENYILDRPFNEFERLWSPASLNTTNSKSKQCDQGEFEEGETSGKEDQSKDTIESILTNGRTNYPETSGGDRGFIAGDPSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPD2</accession>
    <name>A0A5B0MPD2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000404</name>
        <name evidence="2" type="ORF">PGT21_000640</name>
        <name evidence="3" type="ORF">PGT21_023744</name>
        <name evidence="4" type="ORF">PGT21_025090</name>
        <name evidence="5" type="ORF">PGTUg99_010532</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000193">
        <property type="protein sequence ID" value="KAA1066841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000138">
        <property type="protein sequence ID" value="KAA1078711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000276">
        <property type="protein sequence ID" value="KAA1113631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPD2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1113206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1113631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="62" mass="7158" checksum="25AFE9CC152416C8" modified="2019-11-13" version="1">MWGGTQTLARRVRKNLEVDVEISAYRFAKAKEFLNLFSRKAVIIGVRVGFVSKLQYPQNPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MS80</accession>
    <name>A0A5B0MS80_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SnoRNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NHP2_2</name>
        <name evidence="7" type="ORF">PGT21_010575</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MS80"/>
    <dbReference type="OrthoDB" id="5364946at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000026">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="165"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="30"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1079412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="306" mass="33552" checksum="1DC90CB14A2ED7B3" modified="2019-11-13" version="1">MNIQNGIWPILHFGVFFSSRDSGNIFQPIRRQQATSPSQPTRTPKHNKTMSADIEMEEQAATNSKPPSEKKSSKKRKSITTEAPDVENTTQPTEPTTTTPNKKKEKKKKGTTTTTDNEEAENESSKKKKAKQSADAENDADQNINLDAMSPIAHPLADKKLSKRVLRTVKKGSKQRRIKRGVKEVVKALRKGEKGLVVMAGDISPMDVLTHIPLLAEENGSGYIFVPTKESLGAASSTKRPTSCVMISTTRGGSEAIQKKFAEKKKAMVAADPTKAAKIQADEDEYTASFEAVLGEVLQLNEKIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MS98</accession>
    <name>A0A5B0MS98_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_005444</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MS98"/>
    <dbReference type="OrthoDB" id="3257095at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF26">
        <property type="entry name" value="OS04G0435100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1079253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="519" mass="56219" checksum="5545A0D9273C6CA1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MW10</accession>
    <name>A0A5B0MW10_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006289</name>
        <name evidence="2" type="ORF">PGTUg99_026197</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MW10"/>
    <dbReference type="OrthoDB" id="10623948at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137304" evidence="1">
        <location>
            <begin position="20"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="121" mass="13568" checksum="853EFFF5ED339550" modified="2019-11-13" version="1">MRTVVAVIFFLALLQSVSALPTLAKRGEAKPKGELEAKYAVPNDGRLCVLFRMNCDIAPPQFPTTPGWPWWTSPDDYIYPTWPFWTSPSQTIPYTTNNSPYTDAMIGQSLKAVKDNNKKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWG9</accession>
    <name>A0A5B0MWG9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWG9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="176" mass="19397" checksum="B2A7EC4C6801CB36" modified="2019-11-13" version="1">MRASASHRIVLLSHRIRVSNAGPRIASSASEKTMRSIGIASISRCDARYFAGDADVIGRHRLSLPPESPDMRRDSGGVVQSSIKSKPPIAIQSSRLQRYQSIVDRNLALVGPSPINRSLSLVIYRHRSRSYTATDRSSIVVGSSEHPKRSKEALHRYTPIITSLHLLPPTHFASQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXB3</accession>
    <name>A0A5B0MXB3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009851</name>
        <name evidence="3" type="ORF">PGTUg99_018861</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXB3"/>
    <dbReference type="OrthoDB" id="10034502at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="106" mass="11589" checksum="3AFA4E0BD146FB71" modified="2019-11-13" version="1">MFLISGRDETMPSTVDKERFLQRLVDALTSSSNPDHVRLTSDSSFLVIPPEEIIKISDIPRCIVCNSSLRNRNPSTTLPVTLARTGSGTSAPANLSKLPPSIYSMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZJ6</accession>
    <name>A0A5B0MZJ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">STE20_1</name>
        <name evidence="18" type="synonym">STE20_3</name>
        <name evidence="17" type="ORF">PGT21_003197</name>
        <name evidence="18" type="ORF">PGTUg99_030683</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="18 20">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZJ6"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000768">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000385">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000007">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="16">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="492"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="81"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="275"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="384"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="430"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="521"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1082431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1133675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="777" mass="84706" checksum="D798C7BD0D5D7C0B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZJ8</accession>
    <name>A0A5B0MZJ8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Multifunctional tryptophan biosynthesis protein</fullName>
            <ecNumber evidence="8">4.1.1.48</ecNumber>
            <ecNumber evidence="7">4.1.3.27</ecNumber>
            <ecNumber evidence="9">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TRP1_3</name>
        <name evidence="22" type="ORF">PGT21_000360</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="8"/>
    <dbReference type="EC" id="4.1.3.27" evidence="7"/>
    <dbReference type="EC" id="5.3.1.24" evidence="9"/>
    <dbReference type="EMBL" id="VSWC01000126">
        <property type="protein sequence ID" value="KAA1081753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZJ8"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000136">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000031">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="16" id="KW-0456">Lyase</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="19">
        <location>
            <begin position="14"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="20">
        <location>
            <begin position="250"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="21">
        <location>
            <begin position="618"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00018819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAA1081753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="813" mass="88324" checksum="64FE7D803354E6D9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0L1</accession>
    <name>A0A5B0N0L1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014498</name>
        <name evidence="3" type="ORF">PGTUg99_017328</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000408">
        <property type="protein sequence ID" value="KAA1088003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0L1"/>
    <dbReference type="OrthoDB" id="10454495at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1088003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="22113" checksum="6CCF88E77F35E8F2" modified="2019-11-13" version="1">MAPGKQPKNLYQFHYRSQLPNTETRSEKKARLACQKRTKAEIGDSIRRQINRRDTQDQNDQESTNPDNPNTETHAQELLGFNENSLWEDTGEYMNDEDSNSLARIRSLHEEFIQKQKHQHWKDVMDSMFPVYLHLKNQTADWTLPCALDNYSSLLCKCTPDKYKICEVDLIDLMGRWLLAELIFYSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1K7</accession>
    <name>A0A5B0N1K7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNAJ-containing protein X-domain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1K7"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026894">
        <property type="entry name" value="DnaJ_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052814">
        <property type="entry name" value="Peroxisomal_DnaJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006">
        <property type="entry name" value="DNAJ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006:SF1">
        <property type="entry name" value="DNAJ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNAJ-containing protein X-domain" evidence="2">
        <location>
            <begin position="113"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1082396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="340" mass="37543" checksum="D3908593BB55BED4" modified="2019-11-13" version="1">MKAEWAAKQQANGNPQQTIKHDLDSSVAPSSPKNTTPDLRRSPSLAPGSGIQPSPANATTSTTLASSSSQPTGSTAPPTSTTTPAAKPSLKQTPEQRAEAEKYEKQRKEDTINRVNDLTKKLLERIRPLVEAAKPGEPGDPETERFTNGIKMEAEDLKLESFGVELLRLIGTVYYTKASTYIKLHRSKSPFANFLGLPGFYENTKQKGKMIKEAWGMLSSTLDVQTAMMDLEKRQEKGELPEEEMEKLNKDLVGKLLLISWKGTRFESGAILRQVADNVLSKDSPNVTDETIMNRAKALVLIGAIFKAVQPDETDHEERRELERLVAEAAAKKKDKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N210</accession>
    <name>A0A5B0N210_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016396</name>
        <name evidence="2" type="ORF">PGTUg99_012939</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N210"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="85" mass="9559" checksum="89DF6CD81D99A747" modified="2019-11-13" version="1">MEEGDDHSNTYSIYPELSELPNMDYSLDGPAEDEHPIQVINTDENQPAKPQGVQDPDEIDPTDDGALKGLNKIKEFLLDFLKKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N347</accession>
    <name>A0A5B0N347_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ER membrane protein SH3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006390</name>
        <name evidence="4" type="ORF">PGTUg99_024673</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N347"/>
    <dbReference type="OrthoDB" id="5229808at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013248">
        <property type="entry name" value="Psh3/Shr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228:SF1">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08229">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00786">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1082540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1133567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="31427" checksum="0223AAA98C6BD6B5" modified="2019-11-13" version="1">MHSLAFFFSSDEAVADFSSTTSPGQPEQPKTTPPSPSPSALPTLTVFDGVSNALLLSSIGFLLGALFMALTTDYALLYTHAPTTAEAYLAAEQAYLTLWTSPLAVKKAIFHLLLLLPIFTLSVKVSRYTEAAIYFDGLCLGMMLLVLGLYTGSTVPNLRKLAFEPSTQALADLVKNSLNLKIPTDFDPLSPTSVINRLLDLLCHPASILLKPQKALESEQEFKKALELNPVDEKSRHELLAVTAAGHSITIFLLIGIRKVLVPRAVIIHRHVLPIQKAKYFERRKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3E2</accession>
    <name>A0A5B0N3E2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_025086</name>
        <name evidence="5" type="ORF">PGTUg99_013132</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1123964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3E2"/>
    <dbReference type="OrthoDB" id="2497603at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="194"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1083103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1123964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="330" mass="36315" checksum="BA37D12EB4B9C63E" modified="2019-11-13" version="1">MTVQHSPAPTKVKRSHTKARTKRSKTGKTRKLNADASRATDHVAEISTSNPVNALRAQSPTATEEVADSVSYKAPKTKRSATKTRTKRSKTRKLDADASLATNPVTKILTSNPVDALRAHPPTATGDVADSTANDAVGGADTIATQALGKLFESSDAVDNSVLDGFYSIRDMDGFEKWISDHKSTLCNSGFEPDKPNKKTLVLDLDNTLISSDGYEGDFVVDCNNGKRYSILLHPDCITFLHEMVARYAEIAIYTSSSYVYTTKIACQIEVRYARAYPHEDRLPFSKILSSDDCILDGAERKKDLHRLRSEEIKTGEGKFFGGENFWATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5D2</accession>
    <name>A0A5B0N5D2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028759</name>
        <name evidence="4" type="ORF">PGTUg99_022746</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1133019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5D2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409:SF1">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1133019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="112" mass="12788" checksum="27C80EF439C48C2B" modified="2019-11-13" version="1">MSQFYAVRLQEANSSIARLQDEANRLRKGINLQVVRLQEELQQARHDLALKSTEVQDLRHRIEMMQLRMDMQTGGYPGMQGLMRGPMFGPPNNLSSNTAWEVNTNGPHAHTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N793</accession>
    <name>A0A5B0N793_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_024471</name>
        <name evidence="6" type="ORF">PGTUg99_014802</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N793"/>
    <dbReference type="OrthoDB" id="284184at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="60"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1084324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1092290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="382" mass="43540" checksum="7D2E62EC8D70CECC" modified="2019-11-13" version="1">MHCNQPDIKAHFQPAHQTKMSGNQTPNPKDPGSFTRKSLVVSSGHRYSYIDQSLSADGQVVLLLHGFPDLAYGWRYQICDLVNRGYRTIAPDLLGYGGTSKPTDLKAYSKLNSCKALCEILDHEKIRKKVILVGHDWGSALAFRFVQYFHEKVKCWVTICVPPARPGQRGEAPPDFEKIIKQRLPHLGYQLYFMSPEFSEEIHRYRRTIILLLYLHTDRGLENHIQESFARLKDHSFVGENVLREQVQEAAPQLEKIEVKDPEIRYFLSEFEKGGLLGPLSWYKTRDIDHSDEQAASLPASYPAEIPCLYLGASDDPAFPPPLFTEKSKAKLFPGANLTSHIIRGGNHFMHQDPVYRDQVTEVIGNWIDNQLTTAQNRPSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9N1</accession>
    <name>A0A5B0N9N1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N9N1"/>
    <dbReference type="OrthoDB" id="2506484at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="363" mass="39633" checksum="C28554F2632464F7" modified="2019-11-13" version="1">MLLIDADRLIDDPEIQGPNTLKDTNNFGQFNYGSSVVLEDKNTGKPYNHEILLSGYGSRTTAPVRDRVYILGGRFVKNPEEPPTGDEDEAKCTGYTFFYDQALTLGIGGTEQYHTKVASALMNKVGVFGFGLITNRQEIQHSGPNNNVQTDLHVSVQHTDYHTTAKQLMEFQVNYIVPGNRLLNHTFNLFQVGREALIVGYITGHDDDAEIWEVTVLLVSISSGGRSTTVNLMSTPTSSNAGSIEQPRPNMRRIGACKTPTPASKTAPKQFSVNASQEPQQAKRAPSLAPLEIFPTPSTSATVLSPKEKDCEESEEGEVSDDENDDHFSNTYPPGDKNNKRGQASQAGSGVLAEAQKKLKGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND81</accession>
    <name>A0A5B0ND81_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND81"/>
    <dbReference type="OrthoDB" id="10480345at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="198" mass="21163" checksum="5FF0FB767A249413" modified="2019-11-13" version="1">MTLSPFRPQSFHTINISTTYNYTTSTRIKTLLSTFNSITPLDSPVDQSSPKDPSVSHPTPHLFMLRLYDLEDPRSLRGSSFSASSPKDLSVSHPSARVFILRIYDLDDPRSLRGSSSSSSKKNALLVKMAPITRRTASAISENASGDDITARLNVSDGAGDVGHNGGSAASGAESGENNLPQKVVVEFFGFALVGASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NG38</accession>
    <name>A0A5B0NG38_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NG38"/>
    <dbReference type="OrthoDB" id="10361418at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023142463" evidence="2">
        <location>
            <begin position="17"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="312" mass="33934" checksum="354D4A4B890CEF0E" modified="2019-11-13" version="1">MRSFTLTISIISLFHALSFLQSLDLQHLTKRSFLPALKDAQTIAESSKTAAATASASKQLLSLEEHSPVHQADVLSSLHHEPVEQKNLPPINKQQQHQGQHDSSLVDHPHTPSDSQKESTQFLPPIDQHISSETSHDNSLAGHSPTNPSSPSKPNLEKQPTSNELSQISPGSHDPSSSKPPKLNLEKESSQFSPTTKEHSEISPAHSPASHDPSSSKPPKLNIEKESSEFSPTTKEHSEISPDHSPASHPSTPSTPGKPNLEGQPSGKSSKGRLSALRKYSSDFIPKLQRQPKVRPGQFEPIFFDGKVVSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJC1</accession>
    <name>A0A5B0NJC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016957</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJC1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="71" mass="8458" checksum="70E96E2D070B2CDC" modified="2019-11-13" version="1">MLKSTQEVYTDFLTSWNSLNQWIYQIGVAFLKLIWDENCVPYEALSNDSKSWKLSRVNVDFEHFICCFIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJK8</accession>
    <name>A0A5B0NJK8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019561</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJK8"/>
    <dbReference type="OrthoDB" id="2495635at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="128" mass="14762" checksum="A26C27A091A6891D" modified="2019-11-13" version="1">MRIPNISKIFTGGLSLSGTRAPIRSPNWRTRSVGRLEEASWLLTEEVQMGEPSLDRSTSLSKAEFQSHADALKERNEDFEAKSKDRYLWKVPYEWDPPNHGDTDRWLNALLCKLLSMEIQRGERCQPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKP1</accession>
    <name>A0A5B0NKP1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF803-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_008989</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKP1"/>
    <dbReference type="OrthoDB" id="6428174at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF92">
        <property type="entry name" value="SPICHTHYIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="461"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="536"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="611"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="627"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="653"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="675"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1089172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="743" mass="79425" checksum="CE92D676DA9846D8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NM02</accession>
    <name>A0A5B0NM02_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NM02"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="299" mass="32728" checksum="D5C2445D229BAECF" modified="2019-11-13" version="1">MSSVSANEPTSHDRRELEQRVRAPLYTILYRNGMKKQQTLIRQLGKQALSLIGLGLNQLDKIEGLLLLTDGTAAGVVANLGGDVELGDCQHVHDGSSSVGFPKIAHEHTPLTYLVHPHLNFLPSTVPHTPSPKDHRPNNEDQSKYQRFTFPASHLSRVPDPPPTTYFKSIDQNCLADRFEDKSGPSPADDNQSNHRLITFPTGHLSREGPKLTMSLLAPPSVIDTTDQAGPQKGAITLTRLLAQSRRTIERPFKNHPSPLSGANHTAAITARGCFLPQKPQTPPPTTFFEIGPPTPLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMR4</accession>
    <name>A0A5B0NMR4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_016329</name>
        <name evidence="8" type="ORF">PGTUg99_021580</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMR4"/>
    <dbReference type="OrthoDB" id="72053at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366268" description="Thioredoxin domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="653"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="10"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="234"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="141"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="681"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="699"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1070571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1089836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="736" mass="82119" checksum="4E45ADEA1A0705D5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NN09</accession>
    <name>A0A5B0NN09_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NN09"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="93" mass="10377" checksum="124179B3AC9AEDD8" modified="2019-11-13" version="1">MQANVKTVIRIPFRCTIGDLELCSCFGSKPRDQLEFILGTRMDRLWTMVSAALALAFFQLLIGCVCEVYTYLSYVALKSPGFANKMSIGVWSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NU90</accession>
    <name>A0A5B0NU90_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013999</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NU90"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1092781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="80" mass="9308" checksum="94BF4B0B9997B6E8" modified="2019-11-13" version="1">MAVATSLSNAHPKRLQGPGQLFNIRQSHPIVRRSTIQLFENHIIPITWLTASLRFRRDLILATSRFRPLSKELNDRLVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWD1</accession>
    <name>A0A5B0NWD1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_017392</name>
        <name evidence="10" type="ORF">PGTUg99_017724</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWD1"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033846124" description="RING-type domain-containing protein" evidence="7">
        <location>
            <begin position="22"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="305"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1067798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1093455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="367" mass="41182" checksum="AE5B45DC3DB08CA2" modified="2019-11-13" version="1">MSKLLFLSSLVFYALIPMALQIPFNGPNRYALPQGRLSLHHDLDPQAFHNGIPNNPNRGRINNSAHLGTRLLERIRRRFSNCIPGRQRISGRSQQQRAPSTAPRVDNWLDEYSRRPIVEARIHYFPRLRLFKDTDRITNTMLLGYMPRHEYLSPPKERHTTPLSQIPNTLGAATVDQPGGSGIDTSQSRLEGIPAELRGTSNTLNLQAQQTNGCSPKDQDSLFRPKRHHIVPLNQVIKILAVSETNQQGVSSLGTVSNWSQGPLAGVGRIGDSLNVNGKQIGDAGHEDTNQISDVLLGEPDDRTCIACLEEFQGPRQDANNQLIIDSFDRVLLIKGCGHYFHRQCLQKWILGSKRNTCPLCRKKIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWD5</accession>
    <name>A0A5B0NWD5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWD5"/>
    <dbReference type="OrthoDB" id="10434426at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="11284" checksum="C6AE08D5334EA1AE" modified="2019-11-13" version="1">MKALKTPQAPKKTQQTPMKDLKTPQAPKKTQQTPMKALKTPQAPKKTQQTPMEALKTPQAPKMTPQDLMKSLKTPTQTTHYPKMRLKPLNPPKETSSGPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWR6</accession>
    <name>A0A5B0NWR6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_025680</name>
        <name evidence="9" type="ORF">PGTUg99_005530</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWR6"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="376"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="414"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="376"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="414"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="446"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="501"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="104"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="454"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="472"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="501"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="522"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="581"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="636"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1084364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1092248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="649" mass="68771" checksum="6CB1C22305B277B0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZ13</accession>
    <name>A0A5B0NZ13_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_021819</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZ13"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="10">
        <location>
            <begin position="96"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="11">
        <location>
            <begin position="468"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1094451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="841" mass="94181" checksum="BE0FBC17CA20471E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2C7</accession>
    <name>A0A5B0P2C7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035592</name>
        <name evidence="3" type="ORF">PGTUg99_016349</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2C7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="94" mass="9776" checksum="0184A77796C43E52" modified="2019-11-13" version="1">MDASMIAKLQAGLAQRDSLITQLLNRVSAMGLNNARKAPEGSKKKSTSTGAAQSGSGETSKKKKSNKARKQKKTTTPTPTPKAIPKPPTNVGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3M9</accession>
    <name>A0A5B0P3M9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027752</name>
        <name evidence="4" type="ORF">PGTUg99_005840</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1128929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3M9"/>
    <dbReference type="OrthoDB" id="2529219at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="163"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1128929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="365" mass="40259" checksum="313F8026692AA840" modified="2019-11-13" version="1">MAGEDNSFVKQSLTETLTGPSGNSRLAPKPTLGRTRKLINSKDMATRKAKADEESGGILKSLGLTPTARKAHKASPDKEAVTDWEEVDNLDGLTEPTSLHSSTNPSSSQAHHTSRRLAGSEESSKPLKTESDSMHLKSQLPPSSSDSPKHRQSSPKSSESTIVLTLRRQLAESQKAREELASKNAKLERELSELKEDNKVKADGKVLGAREYEELERQFDAQEKLLSGYQRENERSLIELEATKGKMTKMSHLLVKVYGADWEDTISSMNESSPGGPMKQNSLPSTRIHTSPSTIHTTTIPNSPSEKLSLQLAQVQLLVQGMERRLVSREAELEDLHKQTKQEHLSLQDSLNRSLAKSQPPKSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5M2</accession>
    <name>A0A5B0P5M2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TATA-box-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SPT15_2</name>
        <name evidence="8" type="synonym">SPT15_1</name>
        <name evidence="8" type="ORF">PGT21_021727</name>
        <name evidence="7" type="ORF">PGTUg99_033307</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5M2"/>
    <dbReference type="OrthoDB" id="2127950at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04516">
        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000001">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000002">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1095488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1099819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="236" mass="26143" checksum="2D669C0557E88DDE" modified="2019-11-13" version="1">MAQSTFVLPGPPASASLHNNAGSISPDQLTLPQPEGQEATQTSHHPDDPKGIITNVQGIVPTLQNIVATVNLECRLDLKTIALHARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVVTGAKSEDDSRLAARKYARIVQKLGFEAKFTEFKIQNIVGSCDVRFPIRLEGLAYNHGHFSSYEPELFPGLIYRMVKPKVVLLIFVSGKIVLTGAKVREEIYQAFNNIYPVLSEFRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA42</accession>
    <name>A0A5B0PA42_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015727</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA42"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="94" mass="10559" checksum="46965315FD565C72" modified="2019-11-13" version="1">MYLRLVSSDHAWFAFPCPVTTCDRVQSVLGLALAPLRFGRSSSKPIIHPRRFSDSSTIWLSTILKLANYPSPQLSIGSRLAPQQVLRIELEFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBT0</accession>
    <name>A0A5B0PBT0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_036513</name>
        <name evidence="11" type="ORF">PGTUg99_026386</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBT0"/>
    <dbReference type="OrthoDB" id="2498827at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="59"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="637"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="637"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="699"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1098563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1100407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="717" mass="81446" checksum="6CFD763DEB608932" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PE23</accession>
    <name>A0A5B0PE23_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036829</name>
        <name evidence="1" type="ORF">PGTUg99_002128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000461">
        <property type="protein sequence ID" value="KAA1077084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PE23"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="291" mass="33664" checksum="B090638EA7E5CA2B" modified="2019-11-13" version="1">MLKSSKGTQRFSPRIVIDLQPLLKDQDALYLSLLLGGPIKNIIPEVHTHTLISNNADASVSQRVQEDSFESNIYHHVNEVLDGFIIRGDGQVSRTISPKQDLNDDVASEKDHVQSKIEGHPFFEMMASLIEFRARYLQRLSPNEKEQADHLLKDSILPPSFFKTLWHIDQVLTKVLPQKNQANHEARTPWSTIIEKTIEVESQFRRVFIDRYSKEFDAMRARDETPESITLSSQIKFIERQLTKSDLSLIYSIPQRAEEALLISKSELKKCIAYLLFLYLMKQMILQSRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PF84</accession>
    <name>A0A5B0PF84_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGT21_016864</name>
        <name evidence="17" type="ORF">PGTUg99_023490</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
            <strain evidence="17 20">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PF84"/>
    <dbReference type="OrthoDB" id="17725at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000116">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000778">
        <property type="entry name" value="Cyt_b245_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="297"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1095338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1099676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="623" mass="68174" checksum="3FFA9D51EE1ECF4E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJP3</accession>
    <name>A0A5B0PJP3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018815</name>
        <name evidence="4" type="ORF">PGTUg99_002322</name>
        <name evidence="5" type="ORF">PGTUg99_035747</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000173">
        <property type="protein sequence ID" value="KAA1125974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000035">
        <property type="protein sequence ID" value="KAA1136577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJP3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137798" evidence="2">
        <location>
            <begin position="19"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1125974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1136577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="59" mass="6716" checksum="8563C385F225B08D" modified="2019-11-13" version="1">MLFVLVLFFFGMTGYVIGPWEDKKWPGGSRPSFKHKSHTGRWNSNKASYTGSKKKCCVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJP5</accession>
    <name>A0A5B0PJP5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF159 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_032053</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJP5"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="181"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1101875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="30020" checksum="8E15DC19D164A80E" modified="2019-11-13" version="1">MPPTRYFQPVHGSLLNTINARDDKVVEPRGLWNTVKGKKRCIVLCEGFFEWLNKGKDKIPHFTKRTGGELMCLAGLWDSVTYKGATEELHTFTIITTSSNDYLSFLHDRMPVILSDRDSIEAWLDTSSGEWSSSLSKLLKPFSLDDGLVSYPVPKEVGKVGNQSADFLKPISQRKGNIMSFFNKQPRPSGSHSSKQAASSSGPDSKQMEKKGLVGTAPAVLPGLEIEGPHDADTIKDEPKGSPAPSPKKTPIKRSAASVESDALPPEKKVRKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJS3</accession>
    <name>A0A5B0PJS3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycogen(Starch) synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GYS1_1</name>
        <name evidence="2" type="synonym">GYS1_4</name>
        <name evidence="3" type="ORF">PGT21_013512</name>
        <name evidence="2" type="ORF">PGTUg99_035109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000405">
        <property type="protein sequence ID" value="KAA1088927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJS3"/>
    <dbReference type="OrthoDB" id="10541360at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="99" mass="10782" checksum="8042C2B3C955017A" modified="2019-11-13" version="1">MSESKSHINPLLLSAATSEERSEAHMNTTLSKSQDNFTSPVETASPAAPLTAPSAALHSLAPPPELSYALRTQLHDAAQRWALEHGYAIEIQQIWFLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKF9</accession>
    <name>A0A5B0PKF9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_008317</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKF9"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="7">
        <location>
            <begin position="270"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="560"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1101134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="580" mass="63357" checksum="A49B160099AF1E06" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PL47</accession>
    <name>A0A5B0PL47_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_024886</name>
        <name evidence="7" type="ORF">PGT21_026106</name>
        <name evidence="10" type="ORF">PGTUg99_014388</name>
        <name evidence="9" type="ORF">PGTUg99_014499</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000339">
        <property type="protein sequence ID" value="KAA1100679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1123972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PL47"/>
    <dbReference type="OMA" id="ESGSAYH"/>
    <dbReference type="OrthoDB" id="428177at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079:SF4">
        <property type="entry name" value="GH26 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474173" description="GH26 domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="6">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1079867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1083094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1100679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1123972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="472" mass="52451" checksum="F531DECCFD823D19" modified="2019-11-13" version="1">MDLLKLIIFLSFSLTQHVLAAGPALGELDQIYFGVWVDPDLGFQDTPALFNSRLQRNASVFHIAQRMPLLPYNYTTGVGGPAPEYLIENTATDAAVFLTVYPAALSAISDADYTGLGTQLLAYTKDFNRTVFLRWAPEMQGRWNEYGQQPVAYVQAWQTMYKAVKAIAPATIIVWAPNLGHDYPYGQTANIDPTDMAVLDTNKNGELDKGDDPYMPYYPGDDYVDWIGISVYFKQFSRNINAAQPAGFCADILTGIETQTRVNTGFDWYNQFCASKPTKACLIAESGAAYHPNVAGGATELAIKQAWWQDCMTNTTFMQAYPRIKLHMHFEFQKVEADIGPPDVRDYRLTNTTDILTAFQTDLNTVQNNYLWAQYRPITRPIQVNGPGVSAPATSSFSVPTLVLQTARNPQVSGLPTLFGVKRHSGVDKRRTELSRALCVVAASSLLLVSGWLGQNRKRALRRRTQPAPSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLS7</accession>
    <name>A0A5B0PLS7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035835</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLS7"/>
    <dbReference type="OrthoDB" id="2506942at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1102081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="107" mass="11555" checksum="E11D867F07DEDEC9" modified="2019-11-13" version="1">MARELSLQIREFDTSTSLLGCIAHVINLASKAGIAALGTMEDEEDRGDQEISTVDMGASHPTIPPPPQMAISFVTTEPNGANIDANSILKRVHGLCTYVRFSPQRCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PM64</accession>
    <name>A0A5B0PM64_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_014337</name>
        <name evidence="3" type="ORF">PGTUg99_016378</name>
        <name evidence="5" type="ORF">PGTUg99_034361</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000337">
        <property type="protein sequence ID" value="KAA1102725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PM64"/>
    <dbReference type="OrthoDB" id="10624668at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366430" evidence="2">
        <location>
            <begin position="22"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1071654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1102725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="147" mass="14341" checksum="84D6A66E0E4843D6" modified="2019-11-13" version="1">MASIRFVTFLLALSLASGAWGFKCKTAGKTNGVCFPANTGTGTGTGTGTGTNPGTNPGGTTGGTTGSATPAQQGNRGNLSCANKTDKQFCCAQAAAAITGDPAQNTGCAPATAGKRRKAGSFLQKGNQTKAKAKAAKAQAAQAAQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRJ2</accession>
    <name>A0A5B0PRJ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRJ2"/>
    <dbReference type="OrthoDB" id="2508741at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="251" mass="28598" checksum="AE9F9A243B92DEE7" modified="2019-11-13" version="1">MDYQDEIYMCRARINASGVKRHQPPSPLCGISPLQQVAPAPNQAAASSLDPSTATPPLRNRRQLERLPFIDYEAKMSARKHYIWSFLTVPHRGTRHPRVYTLIGRGNLVPSPQRGTLSRPAAVSQGKLTRRESRRPTPLAPRQAGRPQQEEPSDRIDPYARVRAMRDRLPGSRMSTSSQAKRPRQEEPSDRIDPYARVRAIRDRLPGSRMSTSSQARRPRPERPSDRIDPYARVRAIRDGMRGSRKSTKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PU57</accession>
    <name>A0A5B0PU57_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_023458</name>
        <name evidence="3" type="ORF">PGTUg99_026911</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PU57"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137815" evidence="2">
        <location>
            <begin position="25"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="216" mass="22906" checksum="9911FCA12BB29526" modified="2019-11-13" version="1">MAQSSKLTTGLFFCLAGVVNKCLGVGWGEETLFLLPRRSHRSTQISSPPPTNSPPNQPSTSTTQPYPGFPTKALAPTKDTANGLQPSNAAAEVPAAELSKDPAEAAADGPQPLKVTAEDTSDRPQPPRVTADGPEPPKVTAGAAEGHRRRARARTAGRHRSRNPRRSHGSRRGSRRGGSGSCRARDGSRQHRCGGGSAPWSKLWPVCLGHAECLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUD3</accession>
    <name>A0A5B0PUD3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_021654</name>
        <name evidence="6" type="ORF">PGTUg99_030498</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUD3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033849132" description="RING-type domain-containing protein" evidence="4">
        <location>
            <begin position="18"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="136"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1068538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1104393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="357" mass="39886" checksum="55A9A4F949D0CDB7" modified="2019-11-13" version="1">MISRLLLLILLLDRYSAMEPSHGISQLTGKSKVAESGASDWQEEYPSWVASSSHRPSEPVIPPYSTHQGQPYQTEESGWGSYDLDTPEQRRARQRPDEDYSDTESSPRRSYESVYPGEGVVGERDLTSLHYWTSPCDHCGAALGASNRGPSSLPKQLLCEHVFHEDCVAKNPMTDEEICPKCCTSSHSFDQSLAPKHPKELSDILEEFQPILEFLIKLVGKYSGMEHSGRPCPLIASEVAEDVTPELQMDFPTSLNSDPLSLSDPIVPDSSMHGGPLRENDGSFRHSLDIRAPTDGRQLGRPAAVQLGGEARPRMNLERQQRGFVLRERKLFTLLAIGWVILYLIVIELALLARTWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PX83</accession>
    <name>A0A5B0PX83_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PX83"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="112" mass="12463" checksum="37D73B71A629B70F" modified="2019-11-13" version="1">MELRLVAYRKWRPGLFLCLSFRYGLCVASSNVCTLPTFANLLMTITNAFIISPDAETRYVSDDPSRGYGLLQRCVFEPHAQISYLLMDLAGEYVALPISSIVHCLSPSPPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZZ5</accession>
    <name>A0A5B0PZZ5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mob1/phocein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_035808</name>
        <name evidence="4" type="ORF">PGTUg99_024070</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZZ5"/>
    <dbReference type="OrthoDB" id="10262609at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="351"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1106512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1126293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="45441" checksum="010A3A64C9565373" modified="2019-11-13" version="1">MTTNNLEPVFLSSPHHQTATTNYHPSANSSSNQQPSPSASTSTSTSTSTGSSPSANNPQELTRSDSDPIAATGDGHNSNNSSPSPSHQQHYPPPVRLRKGTRFEDAYPSMFASLGDKDPDPSSLLASLESPFQLQEYIAHLVRADPHAVARIIKLPSPHVQREVWIYEQLRRLAHDLGHPLVSALQADCNRAKCPEMKAGEWLYLCAAHATANENECCAIDYIVHTLDGATALLNSARYFPSRLQIPASSIKHFTSIARRLYRILAHAWFHHRELFEECEMETSLYARFLALTDEFELIAEDLLVIPRAVEEEGQHDDEHQQLDEIDEGGDEAQAEAHQDQQQSLDDEREEDHHDQHQQEEQPENTNDHHHLHDSNDSQTVIDPPTNKTDEKTNKIEEQTASLSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q067</accession>
    <name>A0A5B0Q067_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q067"/>
    <dbReference type="OrthoDB" id="10486286at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="310" mass="35738" checksum="2FEC41EC2EAC1301" modified="2019-11-13" version="1">MTSEVKKLRVYLKKLENLVELKPPDTKQEDVLSRWANFFQGYKGSFARLKEILKLTDVDHENQIKQLVKLAHFVRFPTEKEPSGRSQEEMLTRIQQSDKKSRKSQRQLTEFKRFKKFHHYGEDEIAGKFMAIVEAAGVARLQKYHEDLQTLMQSFDIDLSVFRCYWARFYQGGQDSFPELKQRLKLTNLDHKDIIRKFFQLAYFKRFPTRQDSSGMSTDKMLLALGSTKSSLKTTTTDTVERLGWAADIVREGFKAPYRAYDSIVIPTLWSVDGRAAGIGTCPWRTSSRSPAPPTGQAIRLRVSPPNSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2E1</accession>
    <name>A0A5B0Q2E1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007827</name>
        <name evidence="4" type="ORF">PGTUg99_036959</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2E1"/>
    <dbReference type="OrthoDB" id="196858at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00177">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF14">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="2">
        <location>
            <begin position="103"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="863"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="888"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1124937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="981" mass="109108" checksum="F67590EFB37EB0D2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5Z8</accession>
    <name>A0A5B0Q5Z8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH psq-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010528</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5Z8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04218">
        <property type="entry name" value="CENP-B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH psq-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="116" mass="12061" checksum="3D63353B37299536" modified="2019-11-13" version="1">MGCSHGKPNLTTEQKARIAGMVEGGMSHGQVARKVGTGRTTVSAIVARSRAHRGTVETAPKTGRPRLNGQQDLLQLRRALASNPKSTLEEITVRHDPGVRDGAGGLSRGTNPGASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAB7</accession>
    <name>A0A5B0QAB7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_002847</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAB7"/>
    <dbReference type="OrthoDB" id="2500787at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="676"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="756"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="757"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="772"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1109914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="830" mass="94623" checksum="C42EDDD1D4F4945F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAE2</accession>
    <name>A0A5B0QAE2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Carbohydrate esterase 4 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">D25_2</name>
        <name evidence="11" type="ORF">PGT21_016108</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAE2"/>
    <dbReference type="OrthoDB" id="2125469at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471:SF2">
        <property type="entry name" value="CHITIN DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022687521" evidence="9">
        <location>
            <begin position="22"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="10">
        <location>
            <begin position="40"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1110256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="278" mass="31805" checksum="65CCD6BFFC66BC3A" modified="2019-11-13" version="1">MNAKIYLTFLIAFASHSSVQSAHHPKAAKHVVFSTCSKPGTLALTYDDGPYRYQNQISDYLYDRDIKGTFFVNGYNYNCIYDKDVVKHLRHISLIFVPWLNPQATSNLICLHFIFPIEFVLAWSHTNISTLSVHELHQQLDLNERALKKILGIKPKFFRAPYGEHTKENLKVLKQRGYVVVDWSSDSEDSMGASAKKSKAMYTKLAKKYPSPQIALNHETYKDTAHKVTPHAVTVLQKAGYELMHVSECLGMGSNLEDLYQWVGEPSERDVRNFPLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCL6</accession>
    <name>A0A5B0QCL6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_033974</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCL6"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="80"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="606"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="234"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="622"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1110901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="639" mass="70281" checksum="08A59D6F18C22B44" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEA6</accession>
    <name>A0A5B0QEA6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">General negative regulator of transcription subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_002753</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1933107at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000012">
        <property type="entry name" value="Ccr4-Not transcription complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25097">
        <property type="entry name" value="ARM_Cnot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="14">
        <location>
            <begin position="637"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="13">
        <location>
            <begin position="835"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="12">
        <location>
            <begin position="1081"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="11">
        <location>
            <begin position="1417"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="15">
        <location>
            <begin position="1718"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="10">
        <location>
            <begin position="2072"/>
            <end position="2404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="429"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1005"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1605"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1658"/>
            <end position="1680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2417"/>
            <end position="2437"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1292"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="431"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="2417"/>
            <end position="2426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1111517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="2477" mass="276525" checksum="FA8427EC36D43BCC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFP9</accession>
    <name>A0A5B0QFP9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFP9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022715154" evidence="2">
        <location>
            <begin position="21"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1111939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="127" mass="14129" checksum="842D0F0D4B1B66E9" modified="2019-11-13" version="1">MKFAIILCGVLLAFIPTGLAVVVNCAVCKQPGAQEILQVDPERAKCGEVLPNNQVCNEERTKKFFQCVIPSCKEITVKNKNFFVWNAEGCDHENIQPWKNSNPSSSSLAGPSSGTLPQRVEYHEFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHU6</accession>
    <name>A0A5B0QHU6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1112609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHU6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="143" mass="16431" checksum="B23E3AC8CC7AC81E" modified="2019-11-13" version="1">MITNYNIKYIPPGVILEDHYVMTRDIAENIGLTHWRSMKDLDDFLKRKFEYLANEALFKYNYNHLVDMSTIFNGGNLFDGLENYLKSKEAILIENNHIAWDGFLGLGSKYGIPSKTGKRIKEKIAKKEMNKLQGPGQGNKGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QKH9</accession>
    <name>A0A5B0QKH9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QKH9"/>
    <dbReference type="OrthoDB" id="10284401at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="80" mass="8596" checksum="95BE545BB0699A6B" modified="2019-11-13" version="1">MARARNQPRSNGSRTSSGPTGLAPHRRCTTRKETKGAEGEAMAAPSHLLQAIPSPHTRAIKRGPIMRGQVTLVAFFSALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMG7</accession>
    <name>A0A5B0QMG7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006451</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMG7"/>
    <dbReference type="OrthoDB" id="2497224at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="362" mass="41682" checksum="F3FF1DCAC66E8793" modified="2019-11-13" version="1">MAEQTVSIERFQLLLSKLQNYAIRIDQNSLTPRDTAPIVNEGSHQALTTLLLSLRRCVYELSATLSHPLLASRDTLYDLWASFQTLLEIDNTLNHIIALTTAPVLTSMGPIHVPNHENASITMELSASQQEKFLQVFNELGRHLCLYYIRLPISEAKNDVEIASTLRSNVQHLGQCIDEAIEQFSRIDTRAYKSWWHAMANEMEETLRQPVQTLDQEASGPEAEKVQHLCRESIPIVKLTRLLLNKMSRPTNSEVHPISLMEPRNLIALTHSTRMLAIDLHDFVESIHIFDPHPDLFNQEPVENIEEAVEETLQIINGFLEEYSDPNDENFSNRWRDWYERWRDAFDLAVERFQTTLVAINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN81</accession>
    <name>A0A5B0QN81_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013556</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN81"/>
    <dbReference type="OrthoDB" id="2506830at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="185" mass="20775" checksum="5EEC48CDBA890A11" modified="2019-11-13" version="1">MLPISSLARIQKFVAPWTQIMRARLEPLVIFLSFTIIYLSSIAEGYLITTTINQIDDVRAAISKQDINNFHVYTPKLLNQYLFPSGQIDVNAVLALIAARLSYIPFFNEVKVDQVGSAGGCTDSSSKLSSHSTDLSSSTNKSVKAHLHHRASSFKKNLCRKFCSKKKEEEEEEDKEHVKKEGKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPA4</accession>
    <name>A0A5B0QPA4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPA4"/>
    <dbReference type="OrthoDB" id="10584081at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="204" mass="21762" checksum="779BD3828ED189E7" modified="2019-11-13" version="1">MPPKSTNNVTVRRKSRVIAGAPRPMNGSSYSPDEIDSLTPEQVAWVEENPDFWSGAEDDDSGQDEYEDVPQDGEGRYVDNYASEDSKIAVSPSVATGRPAAPSEEPAVVHSDGMDVDSNDTVKHPPRHATYRGDTYPRVSVFAPAELGPPTGARDASVAPPADFARPNVADYGPLPAVELLSCQAVGSWLKGWCFEGCLQVGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPP4</accession>
    <name>A0A5B0QPP4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPP4"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="65" mass="6951" checksum="AB9F525CCBA2C8E2" modified="2019-11-13" version="1">MERYGLPHRKALLVASGAAERAAEHRGGGPKLPLAAEFGSFGRVSPTPAQLHSRQRRADPPPDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQM3</accession>
    <name>A0A5B0QQM3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQM3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="138" mass="15224" checksum="8D21CA5C35193962" modified="2019-11-13" version="1">MRLEADCRSVYHVLVIGCLVDNQSKVAVVTFNPDCIILPLPQVAQPQLSTGQSCGPEEDSSRRMSSDHSGLLPVEPYLASSIPFRRRLQPCPRRAMQKGDEEISAAATSALCPVLQSQIQLKSAIRYLPRLISIYSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QY33</accession>
    <name>A0A5B0QY33_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QY33"/>
    <dbReference type="OrthoDB" id="10605247at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1118232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="160" mass="17317" checksum="7C72255579484B39" modified="2019-11-13" version="1">MLISIPVNTADILDQFPASTFFQHRRSDFPCLDRTYRIVSFAFHQPNRLSRTPSHKLTTESELLLSSSSNSDWFSTSSRIELGDRPGLSAHGKASCLTSAAVNSSGCGGCDSGPSLSAVIAAPVQILLTRPIIRCRFRFRSIPTTADIDSPDTSSTSPPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RCM6</accession>
    <name>A0A5B0RCM6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007884</name>
        <name evidence="3" type="ORF">PGTUg99_008314</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000213">
        <property type="protein sequence ID" value="KAA1122958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RCM6"/>
    <dbReference type="OrthoDB" id="2498032at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="253" mass="28945" checksum="6E9E6A2070596935" modified="2019-11-13" version="1">MSRSRLLRSQSTDDLLSRNLNSYSSGACDSFYRPSSFDYQDPLDYDQGFQSRYDRRCSQSSKANERDASSYHRHPVKERHVTWAPILESATPRGIVPSTLEKRPGSNGTNAYRNSNQSCPIHGYEGQKFTVNRSPNRCKLAASSYNSWPNTTSLYEIRPSSRDISPRCNSIYGSTDPRISRDYSRYENAAYTLPHRYSSFSSLRSPFLNMGYYPSIGSNYHGLVNSAPIPQSNLQRSKWRRSVMGLNRLSLYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RFU7</accession>
    <name>A0A5B0RFU7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020087</name>
        <name evidence="3" type="ORF">PGTUg99_019128</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RFU7"/>
    <dbReference type="OrthoDB" id="2501555at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138117" evidence="1">
        <location>
            <begin position="31"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="219" mass="23780" checksum="C4D3D105FFEEDE99" modified="2019-11-13" version="1">MFQMNIVSRANAAVYVVIALAILCVHLSVAHPKHTSGGQQQCVESIIPGPRDNNQILCEVSKDVYYACEKSNCNSGGSPGEDASVHPFSKMTWDQCALAPGTTRDKKVTGTLTYMVDHKYGIMGTLCLTLNHSSIINLRPVVQHLIYMLSLQASEGPKTASLRTTTTSALRAQTPRDLTAPAVNMSSLAHYECDLARKNSYIQTLLAVWTARRGSANTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RK58</accession>
    <name>A0A5B0RK58_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_000347</name>
        <name evidence="2" type="ORF">PGT21_034162</name>
        <name evidence="4" type="ORF">PGTUg99_017630</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000126">
        <property type="protein sequence ID" value="KAA1081752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000179">
        <property type="protein sequence ID" value="KAA1125385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RK58"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1125385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="72" mass="7828" checksum="503B13D2CC1A7726" modified="2019-11-13" version="1">MHETVHQVQVAGLDLMTGSLAVGYFEGSGETVGREPTSLKRHPLQPQQSFISSPSFNVPLGSSRLKSCRKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RRN8</accession>
    <name>A0A5B0RRN8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein memo1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MEMO1_1</name>
        <name evidence="1" type="synonym">MEMO1_2</name>
        <name evidence="1" type="ORF">PGT21_024868</name>
        <name evidence="2" type="ORF">PGTUg99_004709</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000152">
        <property type="protein sequence ID" value="KAA1127725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RRN8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1093088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1127725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="67" mass="6973" checksum="D962D277E3ACEC1F" modified="2019-11-13" version="1">MAPAHPDIRADADIRLLFPGKPPSASAGGYPPALAGIRAGIFGYPTLKAVGWRALTIAEVNKVCCLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RTD3</accession>
    <name>A0A5B0RTD3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028503</name>
        <name evidence="3" type="ORF">PGTUg99_014189</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000144">
        <property type="protein sequence ID" value="KAA1128133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RTD3"/>
    <dbReference type="OrthoDB" id="10454143at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="99" mass="10653" checksum="1C3FD5D4D52A8F38" modified="2019-11-13" version="1">MSAAYCRATHIPPGGHGSTPRPRRPPGFKAANLTSADRKVWLGRRRSVRYTAVAGSWQFDHKLVDQFAGAVDRLQTGLRSAVKLAAKLAALLTAAARSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RTP7</accession>
    <name>A0A5B0RTP7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_027288</name>
        <name evidence="9" type="ORF">PGTUg99_021772</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000142">
        <property type="protein sequence ID" value="KAA1128405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RTP7"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474922" description="RING-type domain-containing protein" evidence="6">
        <location>
            <begin position="22"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="222"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="164"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="165"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="190"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1066270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1128405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="296" mass="33415" checksum="B0EA66EF252AF228" modified="2019-11-13" version="1">MNISHMSLIVGALVALGYSESKMNSSIEGGIHIEDHLDQTNSIIDGSESTSSHLSQTSTDIDAELYDYRVAYNDYLNEHQINPVQIRQSQSFSSRIQDWFKRLAFATGIKYLRKKTRTVGYMPEKYASGLSHRMKPNLILSISKAKKILKDDITVFKKPHYVHGASSSKIDPGSSTTNPEVLTPKDPTHLSFNDDSESSTSDQLPLEKISKVILVAESQSQCAICQTEFQSPKLKTNSQYQDGLLRFKHNIKPDIQFEAISCVKKCGHCFHPTCIKPWFDLQKKEKKLLNCPLCRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RU51</accession>
    <name>A0A5B0RU51_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Lysophospholipase</fullName>
            <ecNumber evidence="2 9">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PLB1_17</name>
        <name evidence="11" type="synonym">PLB1_11</name>
        <name evidence="11" type="ORF">PGT21_006988</name>
        <name evidence="12" type="ORF">PGTUg99_029495</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the lysophospholipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 9"/>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000139">
        <property type="protein sequence ID" value="KAA1128898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RU51"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000030">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033905535" description="Lysophospholipase" evidence="9">
        <location>
            <begin position="25"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="10">
        <location>
            <begin position="64"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1089097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1128898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="620" mass="67982" checksum="0BCC9C28E98E1FA4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RWI9</accession>
    <name>A0A5B0RWI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002649</name>
        <name evidence="3" type="ORF">PGTUg99_005073</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000115">
        <property type="protein sequence ID" value="KAA1129977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RWI9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="64" mass="6826" checksum="83C66DE117ADDF05" modified="2019-11-13" version="1">MWVETKPSQPLGDSRKAKLSMREAGRADPGDKAGKGGAQGASTNINRPKCPKRSLAKSHYDSVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RXD8</accession>
    <name>A0A5B0RXD8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010001</name>
        <name evidence="5" type="ORF">PGTUg99_002049</name>
        <name evidence="4" type="ORF">PGTUg99_034227</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000137">
        <property type="protein sequence ID" value="KAA1129174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RXD8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366681" description="Secreted protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1129174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="13919" checksum="49ECDD5029113898" modified="2019-11-13" version="1">MLKNCGTCFVLTALVVVAFMNGLCLAGEDVFKAVHGQPTRTGWREGTGVFLPKPRPRTGVSSGSRGTSQRQYETASSRNTHYGNIGSQSYGTGPFHPQNGHQGAKAPSHGAENDKNDVNKLPDDLYTDPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S5P2</accession>
    <name>A0A5B0S5P2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033497</name>
        <name evidence="2" type="ORF">PGTUg99_026668</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000073">
        <property type="protein sequence ID" value="KAA1133210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S5P2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1109124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1133210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="231" mass="26069" checksum="E1119A56EAF8CB0F" modified="2019-11-13" version="1">MSVHSWIESERHFDSNRVLERSVADSIKKFIYLLYDYFKHLLILNEFLFQAASPAFLLSTYASPSIFILNTVLPTYAIPSRSRSQVISIPPPCSIQNEIKGSFVDLVRTCLNLIVVGGRISLVSTRPTLEPNNHRQFKRSPSHSKVTDQTPIFIDSSPAISPLAEEMEISMLLTPPGYSIYDGDCFTQPEFDALFVEGHRVAGTECHRLCLVTRELTLRTNVVCIQCRKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SIY0</accession>
    <name>A0A5B0SIY0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Ribosome biogenesis protein NOP53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_002765</name>
        <name evidence="9" type="ORF">PGTUg99_013735</name>
        <name evidence="10" type="ORF">PGTUg99_025681</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the NOP53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000206">
        <property type="protein sequence ID" value="KAA1123753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SIY0"/>
    <dbReference type="OrthoDB" id="5072at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011687">
        <property type="entry name" value="Nop53/GLTSCR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211">
        <property type="entry name" value="GLIOMA SUPPRESSOR CANDIDATE REGION GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211:SF7">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07767">
        <property type="entry name" value="Nop53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017302">
        <property type="entry name" value="Gltscr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="246"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="195"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1067373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1123753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1137852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="431" mass="47921" checksum="82C09969486549DF" modified="2019-11-13" version="1">MPPTKSKSKYSRTDPSLTGSSIFEVDTSGSASIRHSVLHGAFASRARKGVQRTLRVDQILSETASQGPPPVSGRVRGATREVAKTKFLTASEKKRLEQMVIRKKFDSGINSIDPVKRNGTAVAVKPPQPPGGDIWTAAHSTTPAPPITSKTKTSIKPPASLKPNETLTEVVNTVPAAGLMPIPLPHPGQSYNPALSDHQAILRQTLEKLEEEQQEADRALEVKQRVTQGLKITQAKSPWEICEEAVGDGESGDEQPNAAEGSSPKSLAKKKIPKKKTKAQRRRQEREREQMRQHNLRKEARRVAHTLHELPKLVRGLSVAEREARQARIERKARRAALVAEYGLRAVRGGKPKGLQSVEDRHEYQLTEDLTESGLRGLKPEGNLWRDWSQSNTRRGKLDSRPKVGLLKGQRFQKGRMFKEVEKHAWKNFEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SJI2</accession>
    <name>A0A5B0SJI2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023094</name>
        <name evidence="4" type="ORF">PGTUg99_019744</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000004">
        <property type="protein sequence ID" value="KAA1137987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SJI2"/>
    <dbReference type="OrthoDB" id="2505955at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="111"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="182" mass="21165" checksum="771EEEF572D7379E" modified="2019-11-13" version="1">MLKNKKRKEGCKKRWRQKTRKASGNEASTEIKKGLYQFTARPSPVSLYDEYRQRRKKKYLTPASILQAANFIKAPGFRIFNRPDSHVMIFDEYNQNRLVGIFQFTPFSKMTPDQREDLDFLAGFFHSHKKYVNPVSNFNSACLGGKMNMLGWRKCMKPNERAGLFLSQAKINKDVHGFTSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJI8</accession>
    <name>A0A5B0LJI8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJI8"/>
    <dbReference type="OrthoDB" id="10545189at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="614" mass="68171" checksum="6B949BD09EA6AB57" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LK37</accession>
    <name>A0A5B0LK37_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LK37"/>
    <dbReference type="OrthoDB" id="3237202at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223:SF5">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="443"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="593" mass="64844" checksum="01F436B640BF2129" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSE5</accession>
    <name>A0A5B0LSE5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033661</name>
        <name evidence="3" type="ORF">PGTUg99_016490</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSE5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366039" evidence="1">
        <location>
            <begin position="31"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="76" mass="8349" checksum="F8C5200B9D85AF81" modified="2019-11-13" version="1">MKILPELAHWGLTFTLVVFLLQNAPKYKCATQCRDGHFASGYPLISGYPLLISAELGNFAEIKSGYPDISGYPDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSL8</accession>
    <name>A0A5B0LSL8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSL8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="209" mass="23844" checksum="A774B541AAEFD787" modified="2019-11-13" version="1">MRLEGPASLIDDHAGHRAAPLPLSVPECFCPHCSQYRLQVDRKHDHDYAALHEHRPPIDSKNLRSLLDDKSNLVEYSNLLSEEEGLLSSDQSDHLLVKLIIWTAARNIRDAANYSSREDYKDIFFFLTLLRLHKALPREALRIHYAVASIAQNLLNMNHQISNIPTAISFGKLCSESDGILTTWLYSMWDADEADSYHFRSLGKYSDPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSV6</accession>
    <name>A0A5B0LSV6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nascent polypeptide-associated complex subunit alpha-like UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021155</name>
        <name evidence="2" type="ORF">PGTUg99_003528</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSV6"/>
    <dbReference type="OrthoDB" id="285219at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14361">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038922">
        <property type="entry name" value="HYPK_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nascent polypeptide-associated complex subunit alpha-like UBA" evidence="1">
        <location>
            <begin position="79"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="123" mass="13520" checksum="05694DC11216B2DA" modified="2019-11-13" version="1">MSTSTARLGHVPGAKVIAYYADADGQFSSNKIGYDVDLYDEAVRTIAFGELRTKRDGQEDLGKLDQLSITEPEGEPAPLKKEDVQFICVQLEVSHKVAEKALRLAKGSLEDALLHLVYQADLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVI2</accession>
    <name>A0A5B0LVI2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="13">dUTPase</shortName>
            <ecNumber evidence="5 13">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_050360</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in nucleotide metabolism via production of dUMP, the immediate precursor of thymidine nucleotides, and decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dUTP + H2O = dUMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:10249"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the dUTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="5 13"/>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVI2"/>
    <dbReference type="OrthoDB" id="419889at2759"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.40.10:FF:000007">
        <property type="entry name" value="dUTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001862">
        <property type="entry name" value="PRK00601.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241:SF0">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="15">
        <location>
            <begin position="18"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1068947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="153" mass="16077" checksum="0A790F62EBA6B59B" modified="2019-11-13" version="1">MAASMSDILQVQRLSPDATIPTRGSKFAAGYDLYASKDTTILKGGRAVVPTDITIAVPPSTYGRVAPRSGLAVKHGINTGAGVIDEDYRGPVGVVLFNHGEQDFQVKKGDRIAQLVIERIVTPEVREVQSMTETERGAGGFGSTGGFGTANQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWM2</accession>
    <name>A0A5B0LWM2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWM2"/>
    <dbReference type="OrthoDB" id="10320520at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012808">
        <property type="entry name" value="CHP02453"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09365">
        <property type="entry name" value="DUF2461"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="87" mass="9674" checksum="DFAB985276CE0D4D" modified="2019-11-13" version="1">MDLLKGLPLPQNNNQGWLIPTRLEAIKNAIMKNPKPLRDIINHIEFNELVDQVNNAILAQVDQMNNAPNGVNKNHPDQYTGGVPVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXQ7</accession>
    <name>A0A5B0LXQ7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="3">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">GUA1_2</name>
        <name evidence="18" type="synonym">GUA1_1</name>
        <name evidence="18" type="ORF">PGT21_017616</name>
        <name evidence="17" type="ORF">PGTUg99_035081</name>
    </gene>
    <organism evidence="17 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
            <strain evidence="17 20">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of xanthine monophosphate (XMP) to GMP in the presence of glutamine and ATP through an adenyl-XMP intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXQ7"/>
    <dbReference type="OrthoDB" id="1724632at2759"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000002">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000001">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000001">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000848">
        <property type="entry name" value="PRK00074.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54810">
        <property type="entry name" value="GMP synthetase C-terminal dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7 15" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 15" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="16">
        <location>
            <begin position="280"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="308"/>
            <end position="314"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1068663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1104267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="611" mass="67702" checksum="E0006329CF569249" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZD7</accession>
    <name>A0A5B0LZD7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005033</name>
        <name evidence="2" type="ORF">PGTUg99_036610</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZD7"/>
    <dbReference type="OrthoDB" id="10526340at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="393" mass="43442" checksum="DF1E192804A56521" modified="2019-11-13" version="1">MAYPWGEVYLQPVWKERCALEDFLFFSAPRRVLLRFVVRGNLLDGLVVPPLSLLFCLEVHPSSVSHTPVPYYFPRCHPLPPPRTPHVTPPTPAGPGTPPAARKQVSHRRFRLWSAPNPASAPRCSPLFVAPPQYTLNPVPQSAVIPGVRRCPDSPHFPQPACVASAPRAFRISAASIPHAPNKPAKALAKIKMNKIGHQSSVALALAITIFHPPKHSSESSRTISMSAHKFPMSSSRCISQEILPEAGFTWCTLRMTIQTQGLIQFQASDKESILNSNGGEGWRRARYVPLETTNAHTGPRKCLSSRVALYKQCEDRQEHKEVRTHIAAQVASATARLIVTPSLIPKPSDTYQTSVRGSVKQGYPAADHRRQKWCTKEIARGNPCWPSSPDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3G5</accession>
    <name>A0A5B0M3G5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019072</name>
        <name evidence="1" type="ORF">PGT21_024267</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3G5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="231" mass="25464" checksum="BD32A39E1337A92D" modified="2019-11-13" version="1">MYQPCHRSDRARSEICVPSPFFTLLVRSVPSISQSIPPPHLSSSHNSTDGLSISTGMRTYPFFDRHSTDSVSSILLSFSFRMFMTVVIGLIGRPHSESIERYHKILCASLLPQCLCIVGNCRGPSSLLSIPLQAPLISSINTGIVSSFSASPYQLTPFASYVHSTSKHPRFHQIVSSVISLLSTVTTMSSKPLPRPRLLKSRPLVATKQHLIQLPSKIFLAIRLQAFDSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M498</accession>
    <name>A0A5B0M498_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M498"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="525" mass="56618" checksum="7DF9A3A282303075" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6F6</accession>
    <name>A0A5B0M6F6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6F6"/>
    <dbReference type="OrthoDB" id="3142841at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052979">
        <property type="entry name" value="Adenylate-forming_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="195" mass="22194" checksum="07B13A439D7D9CDA" modified="2019-11-13" version="1">MKPASGQESKETEHTEILPLSLLITGERLSSLSFPSTFSKTLSSHDYDSNEKGSHSSGEVIEKGIVLPPSPRPAPHPSAHTVDSSIKFWTWFSPYRQMLFLVVILQAVMILVTLSGGWHFARTHSSGFVTGNLLVTIAIRSEWVMRLLYWFSIKLFRIWTPLKFRVIVVAILYHIGKSHHLIRHTARFLWKLTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7N0</accession>
    <name>A0A5B0M7N0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029571</name>
        <name evidence="4" type="ORF">PGTUg99_025431</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2499190at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018849">
        <property type="entry name" value="Urb2/Npa2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10441">
        <property type="entry name" value="Urb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal" evidence="2">
        <location>
            <begin position="1325"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1520" mass="171978" checksum="7D95620EF6C0494B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB90</accession>
    <name>A0A5B0MB90_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB90"/>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="183" mass="19876" checksum="7C02AD23EBAE43D8" modified="2019-11-13" version="1">MSNLDQEKFENGNVVAPSVGLYETEKGTPVGKSANAKGKGKALESKSVSKKRCGASTPDAQLKQLGHLLQENEYLLEEALTIDLGIQNLESHGGKLVGTRNEVLSALKILKKWIKPQSVKTKLTWLIAKPWCYCLVHLSVQLLAETPSFSNPLKTGMSTVVGKYLDPRNICVVNGGQEQSTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB99</accession>
    <name>A0A5B0MB99_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB99"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="108" mass="11554" checksum="25889EFC00044654" modified="2019-11-13" version="1">MLQVAAAPEVVSIKCWQCHKRTTVHERCQTELACGHICNFIKSTNCDDCKHNIPTGHDHARPAKCPSCGTRGNASTANASPSVSRSSSSRSSASSASGYSVKRYTDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBE6</accession>
    <name>A0A5B0MBE6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBE6"/>
    <dbReference type="OrthoDB" id="2518661at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="13800" checksum="3B4E0F37326A9942" modified="2019-11-13" version="1" fragment="single">MSTLKSIRWQRLRYYPKTHLYPTVPFDKEKYKPLRHPTPDEVSKACELVDRKFFLMNFGRVVLVDPNDEDSVIAVMEFTPWDQLTETDKENLNFISSFLHQSKEFVNPVGSSTRSWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBJ6</accession>
    <name>A0A5B0MBJ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020808</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBJ6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022832221" description="Arrestin-like N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20071" checksum="77332A56FD63E72B" modified="2019-11-13" version="1">MAPKLWRLITMFSIVPLDLAQRVSDLIPGTVVHISAYLLKHNNGIVDIEADVLTPAPRDSGPNARSWLVFEGPIHAESTTSPITVHATASNYYHEIPLSYRLPEPQIYGQLLMHILIDSDTVSAKSNQVMVSIPVPRFKSTHPDAFDSAGPDIYQYFWINGIITNTKESFIYVEIDALIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDS6</accession>
    <name>A0A5B0MDS6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005332</name>
        <name evidence="2" type="ORF">PGTUg99_030786</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDS6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366121" description="4Fe-4S ferredoxin-type domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8470" checksum="CB9FB8B345126A64" modified="2019-11-13" version="1">MNRLALSTIVVAAALVSVSVGLYTTDQKAIAGHDVEAMGSMCEYDDDDMRVFGTNHDASGRCQGCRNIGGACGEFCPANNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MF19</accession>
    <name>A0A5B0MF19_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YCII-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MF19"/>
    <dbReference type="OrthoDB" id="5519740at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1060">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051807">
        <property type="entry name" value="Sec-metab_biosynth-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606:SF3">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="19777" checksum="BEE535404279856A" modified="2019-11-13" version="1">MPSFVIGSSIRFLRLLELQSSFNLPSHPRSFAKTLARTTIMTSPKKEIDPDVNWSSPLPKQTPSDAPNDAKEADDAKVFTYLVVAPDLGDSKRAILRAEHFKMAQEGLKTGRIVNAGAMLESDASESNNFDPKMIGSWLLIKARSIEEARRLCLNDIYAKESAWDTTKLQITPVKTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFS4</accession>
    <name>A0A5B0MFS4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFS4"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038921">
        <property type="entry name" value="YOR389W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204:SF1">
        <property type="entry name" value="ENTEROTOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204">
        <property type="entry name" value="YALI0A21131P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022758979" evidence="1">
        <location>
            <begin position="24"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="468" mass="52962" checksum="655CAC08DBD0F4E4" modified="2019-11-13" version="1">MKLQGVLLRFLGSFWLVSTLTRAIRTGREQIALSSSNFSAYKTENSNLIFSSFAGLLQQWPNSFAFSGHSIIPGLIPRSTLLYHATNNINGPPTGIEWLAFNSEMSYSILSHRGGELDLYKYAATRPLRVLYVDGQSASLGTPGFMDSQYIIIDASIPPDNPDPGHDPTGETARAVALCKMGEKYGFEGVVRMATSFELLWCDFKNGIELLNMTNATDPYYKHQWDSDVPTNSLERDQSAISDSADPPFFARARWTYAHSGAREFFAPGEVRVILDPMGFISFYDGLESLDAKRRVEGTSEGPRARHRLYGISAHDTKKVQARLIEVLERKEAEDWQIDSHRPDWRALVLTIIQRYSQPLIELRYLLNREDLNATEQAIEVRGLTYDILMPSLDFSHWSSPERDWISEGIHRCLTYYTSTPLLPSDLTHSIRLIIGAIEGTLKRVCTTIYTLFFQTIQMFLPVEPTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGH7</accession>
    <name>A0A5B0MGH7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_029325</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGH7"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1075116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="151" mass="16824" checksum="B68EE86110891BF2" modified="2019-11-13" version="1">MEVPKKTQACNEDELKGLIERQGLKKYNQPCKPRQVETVASLIRGRNTFLLAATGFGKSRIPELYLDLLCKDRSGDIQGVVVVLNPLDALGDNQVAEKIQAGFTAINLTQLNFSEEAEIDVKSGNYNFVYSCARQSTVTVFFMSEMLTKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIY2</accession>
    <name>A0A5B0MIY2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012304</name>
        <name evidence="3" type="ORF">PGTUg99_013739</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIY2"/>
    <dbReference type="OrthoDB" id="3265918at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="492"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="507" mass="56801" checksum="8DADC4BCDDE54C62" modified="2019-11-13" version="1">MSLIRLTRSYSTTTTTTTTIKHQFIHRPTKPKLTSSNDIQFLPPAKFTLSIISLSYNRPNVHALLSQSWPASRSIRHIDPNQLFILTAIPARFVNQNQPIPNHIHTLSSCFSQQDQERWKERRIEKRKSKRKPTHQLRLTFSLRIKELHKSAVIRKSIRKRWIAALKLIVQYGASSSRSTECTSAAAAAAADPKKNSVERRMAEGEEVRIDPAQAGFHRWLDPDHYYIVHPTLALNTVGLPQLIRAIREALHAIHPINNNPRHSRDTPSSTHPSRKPFLRTTHVASSPQSQSGSSSRINNQHAPPTQSNPFIIKPHSHSLSSESPSRRLPSKHPQFMCPQQTQSSPASFNKPRNTSTGSCSDHPPPFSPPRRRLPTSDPKFVSPQPTRSGPPASVHMKPSAPTSVISTTPADHSLKSSLEPPRKILPSRPIFTPPKQNHPTTLYKPRISSTGPGSHHHSSFEPPPRKIPSSQPKFFIPPQLSLNTVTTTHQPSSLSPPRRLSIPLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MK71</accession>
    <name>A0A5B0MK71_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014571</name>
        <name evidence="2" type="ORF">PGTUg99_024344</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MK71"/>
    <dbReference type="OrthoDB" id="2506088at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1126817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="509" mass="57992" checksum="F7CC4FA0E5AC4AD9" modified="2019-11-13" version="1">MKYDIRASKDGATAANNAFTERISLLDQTNRERLFNPFFQVPDTPVEVLHVFLLGIVKYLVRDLMGRMKPAQLDLIEGRYRAFNLAGLNIPWLSPHYMAKHSSNFVGKEFKAVLQSAPFVLFEFMDDPERQAWIALCQLAPLVFQTHIDEMRPYLALLRFHIEKFLYYVIKITAQWVNKPKFHMLLHLPDSIERFGSASLYATEKFESYNGTLRKAAVHSNRQSPGKDIANSFASYKCIRHLTCGGAFKDPKDSTRYITAAPSVANMFMERHSFQKSMDYNFLAHTELKGHFPRVINSKVTAPTKLPSGLRMHLPGRRISQLEAIQWTAHRVLKRGVYVMVRSGGVLGGTALTIGCVDHVWEARFRGRVSFWVCYTEYSRGGVDPYYQMRSIRKSKTHKYVHIEGVESTINIQHNCHAGGCQVSPTGQILIEREESKENNVIVVHEDTGEYIVNSGELNGRKVLRDWVDVPRGEEDIRDVIPTLKKGLATWVQAASGSEEDEDLDDPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLE6</accession>
    <name>A0A5B0MLE6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycogen [starch] synthase</fullName>
            <ecNumber evidence="7">2.4.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_008416</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18549"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:18550"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyltransferase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.11" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLE6"/>
    <dbReference type="OrthoDB" id="6335297at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008631">
        <property type="entry name" value="Glycogen_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05693">
        <property type="entry name" value="Glycogen_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1077452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="66" mass="7669" checksum="F97449B8A767F4C9" modified="2019-11-13" version="1">MEVEAIEPDMPEMRDSLQSMKERGGGPRVLLFDTGSMMSWLDEWKNELWNLAGNNTIVFGYLVVWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMU4</accession>
    <name>A0A5B0MMU4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMU4"/>
    <dbReference type="OrthoDB" id="2510110at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="501" mass="58780" checksum="724C3AE2E95AC3C1" modified="2019-11-13" version="1">MLHFYPYIIPVSHFSQILNLSFIHEQSQPLFWKYLNGWTDHQGAGSQLLAQCLQEFCGYFWNGKAFDNSFKYHQFVFQISSRIRKHLETRTDGSTSPGQKSVEEIEAFFCKAQLIMNYVEEFSQTVKKNQWISRFHPRPRASLAQLRHLAFLICDHMSPIYKFEAEVNVFNISQELELHGFPYCIQHTMLAIMGNKHYSFGDLRRTVLAIMEQAEQKQTDWIGKLYERLEMTVSQVVMSCQSDVQPRADGVYMKQDGFSEEIVARLTELEHFLIQELSYNEVQNKLLSWDKVFLAVRQELSSASSENVDALIRLLKHGHRVRRNLPVFHHAYYLMTQTNEREKKIVFEVMRHKLKELQKEMPTHAFERIREITKLTQAISRQTLYKVWTDRLLGKLTKLPHDLAMTVDGLMADCSRAILLKKMDAVCADYRVQTILDPVRDRLNLDARNLLRRARLLLASKRVTDEVHIMAKMLFHNAFADFSLFNVGDLYLRFLSLVNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MN79</accession>
    <name>A0A5B0MN79_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MN79"/>
    <dbReference type="OrthoDB" id="2508781at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1078281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="138" mass="15503" checksum="4461D615D3609C8E" modified="2019-11-13" version="1">MEEIAEVGEQNLGQRTGRLFNHRGSTSSDVKSEGLADAPENLTSNMEPESNGLDPQENVNGNQLLHNVQSLYWYWALELLMLALAGPLMKHWPELFEPRQSTYNLIPGLWIAAAPGMAWNFFRGSKFISKFISKASHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRL3</accession>
    <name>A0A5B0MRL3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRL3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8829" checksum="F0DE5661E81BAB90" modified="2019-11-13" version="1">MRTGLLRNLTLQQESSNQSCSPTSWTRQLTVPASKHDQTLDIYRQYRGLLSDESTTLRKEFEDLLKESVLISQIKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MS24</accession>
    <name>A0A5B0MS24_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000134">
        <property type="protein sequence ID" value="KAA1078816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MS24"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="164" mass="18439" checksum="9D3A8F28205C7119" modified="2019-11-13" version="1">MIGQFQNFQVLKLCDCSTQQRFNPTLRNPSFGPYHPSLLHPNQTRKGSPSQLTLTDTGLVDSQQHRPDSDLPVNSPALPASCYLAHCSSPLMALYNFTHHSFLFNPPASHEFTGTSRYLLFIALQSTSLTRAYWHIACTTSSSIPYNFTNRYCTTSSINPFSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSK3</accession>
    <name>A0A5B0MSK3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Shieldin complex subunit 2 first OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSK3"/>
    <dbReference type="OrthoDB" id="2506307at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049507">
        <property type="entry name" value="SHLD2_OB1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21669">
        <property type="entry name" value="SHLD2_OB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shieldin complex subunit 2 first OB fold" evidence="2">
        <location>
            <begin position="250"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="386" mass="42644" checksum="3EAAF200DF84B930" modified="2019-11-13" version="1">MSTKRFILFGNPPTLQQLRAWSISSPDIPITWQTRTVYDSTSQAHDHQSGSKPDCTTDQLVANSHQAHSSDPPRHPSSANERSTRLPTLPSASVGHSKHPGDITASSESLDLNQGSRKRPRLQTPEERDDEVDTDQDVSKDFPGNGHDAAATNSDSMDYYQSQLDYRDSFDEVSGDISSLTPTPGNRTSCLLSNPPQELTNDPSPASGTLRPPSQQQSQSYSESMVVNPPQWHFDSRSCTPLDQLENHPKSPYSILFYLSDKKGLSTIELKKPRKDGSSAVDIAHFECIDDSGSSLKLVLWDDVAESLDRACSVGDIIYLSGIAISTYQGKPQASSTFGTRAQICYRLRPTHASHLLLYCPDLRLAYDPTTRRVSQLVDWAKSAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSZ4</accession>
    <name>A0A5B0MSZ4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="5">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MSRB5_2</name>
        <name evidence="8" type="ORF">PGT21_005297</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSZ4"/>
    <dbReference type="OrthoDB" id="44061at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081:SF8">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="7">
        <location>
            <begin position="68"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1079248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="204" mass="23370" checksum="754556690935188A" modified="2019-11-13" version="1">MSLLKPTQPLLRLLLNRQWTPTSQRIRLRPQTQTSLSHPPQQQSFSSHTPHNHQENRMTESSNLPKTEEEWRLRLDPEQFRILRKKGTEMAGTGEYNKHQPDHGIYECAGCAAPLYRYDHKFNSGCGWPAFFDAFPGAVSRHEDRSFGRVRTEITCTNCGGHLGHVFKGEGYGHPTDERHCVNSISIKFNESTPAAEDKLNPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MT51</accession>
    <name>A0A5B0MT51_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MT51"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022775534" description="RING-type domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="16418" checksum="EA242DE814E104D5" modified="2019-11-13" version="1">MLNLIVLFSPKVFLVSIPVCGLPFEHGASSSNSFQKLRQSCKSIKQKCTSSVRSGLRRLSHFGSSSTGPQEARLCSICSTAMSDRQDVFMWPTCVEGHAVHRGCVDPRSIAASSCPICQAVAPSILVRQVEHQEERTPVSDLHHFRSQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZI8</accession>
    <name>A0A5B0MZI8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002647</name>
        <name evidence="3" type="ORF">PGTUg99_031357</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZI8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052087">
        <property type="entry name" value="RRP12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="166" mass="18481" checksum="43E8FFAEAA6B7AF8" modified="2019-11-13" version="1">MIIDEKESSQNKSTKNGNDESTNAFLEAVHGADGFRRDARGNVKANKKRKLDQDVVDRLEQDEEQQLDIQDAVHGLQITDDQKTGSTHKKKKTKRVKEKLGSEFKAKKAGGGDKVKRNKDGQEVSPFAYLPLQSLYSKKKLKVTKTLNITGKVKGSASHSKSLPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4B4</accession>
    <name>A0A5B0N4B4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_013788</name>
        <name evidence="3" type="ORF">PGTUg99_025162</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4B4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137377" evidence="2">
        <location>
            <begin position="21"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1094218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="54" mass="6162" checksum="AA46A9BA3AF5F2B7" modified="2019-11-13" version="1">MLVRLSLVAFILLHLQATLGAPRGSMNRRSNDGYTWDHDDPDGPYDCKKFDNCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N586</accession>
    <name>A0A5B0N586_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SNF1_1</name>
        <name evidence="13" type="ORF">PGT21_003251</name>
        <name evidence="14" type="ORF">PGTUg99_013974</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000209">
        <property type="protein sequence ID" value="KAA1123360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N586"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12122">
        <property type="entry name" value="AMPKA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000544">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000236">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000021">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032270">
        <property type="entry name" value="AMPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16579">
        <property type="entry name" value="AdenylateSensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="35"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="308"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="398"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="487"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="413"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="487"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1083664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1123360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="697" mass="78545" checksum="4E3E1AF403DCFF0F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5F3</accession>
    <name>A0A5B0N5F3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029297</name>
        <name evidence="3" type="ORF">PGTUg99_025068</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1133038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5F3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366221" evidence="1">
        <location>
            <begin position="25"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="73" mass="8168" checksum="29891298CFDA1356" modified="2019-11-13" version="1">MNFLQRLYLTSAALALLAIHSAESVAVDCDLHFERSSDPLKTECWSRLNEGYYCGIASCMNVADNNKDCELHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6G8</accession>
    <name>A0A5B0N6G8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_005342</name>
        <name evidence="8" type="ORF">PGTUg99_014994</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Regulates mitochondrial small subunit maturation by controlling 15S rRNA 5'-end processing. Localizes to the 5' precursor of the 15S rRNA in a position that is subsequently occupied by mS47 in the mature yeast mtSSU. Uses structure and sequence-specific RNA recognition, binding to a single-stranded region of the precursor and specifically recognizing bases -6 to -1. The exchange of Ccm1 for mS47 is coupled to the irreversible removal of precursor rRNA that is accompanied by conformational changes of the mitoribosomal proteins uS5m and mS26. These conformational changes signal completion of 5'-end rRNA processing through protection of the mature 5'-end of the 15S rRNA and stabilization of mS47. The removal of the 5' precursor together with the dissociation of Ccm1 may be catalyzed by the 5'-3' exoribonuclease Pet127. Involved in the specific removal of group I introns in mitochondrial encoded transcripts.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds to mitochondrial small subunit 15S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000214">
        <property type="protein sequence ID" value="KAA1122925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6G8"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF17">
        <property type="entry name" value="OS12G0638900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="5">
        <location>
            <begin position="424"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="767"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="768"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="780"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1083728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1122925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="839" mass="94593" checksum="7433B4C2E52A1376" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6H7</accession>
    <name>A0A5B0N6H7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Autophagy-related E2-like conjugation enzyme ATG3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ATG3_1</name>
        <name evidence="12" type="ORF">PGT21_016997</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6H7"/>
    <dbReference type="OrthoDB" id="1584384at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="338"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1084083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="405" mass="44172" checksum="15952B1ADFFF1E0C" modified="2019-11-13" version="1">MQSFQQFTQTHFWAVRDYLAPVLRESKFKEHGRITPEEFVAAGDFLCYKFPTWSWEAGDGTKRRDFLPENKQYLISRNVACLRRASQMVYTDKDEDAETMMSFAAEGAVGADDEEWAVTHTTRPNQSAAHDIGDIPDLDQNVNSLSLSVDNGAGRNPDASNNADEAIPNMDDIPDMDDDLGDAGGVVEAEDDATASAQAAANQQAELGQNNPSNLVSVRTYDCLITYDKYYQTPRMWLMGYDEHKRPLTPSQTFADISSDYAQKTVTIEPFPHLNGLNLASVHPCKHSSVMKKMIERMDGSFKEAQQKHSASSGSGNSGSVSKKKGWIGGVVKKATGGASIGGGSSKDKKAATPTTSPTSTTSALGPESQEPEIEGLRVDQYLLVFLKFISSVVPTIEIDSTTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9X1</accession>
    <name>A0A5B0N9X1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N9X1"/>
    <dbReference type="OrthoDB" id="3251205at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="148"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1007" mass="115815" checksum="8DA5E38B6FE54D74" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NCQ7</accession>
    <name>A0A5B0NCQ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DEAH-box ATP-dependent RNA helicase prp22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PRP22_2</name>
        <name evidence="2" type="ORF">PGT21_009845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NCQ7"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box helicase OB fold" evidence="1">
        <location>
            <begin position="27"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="133" mass="15325" checksum="A6F3D53ADBCD876B" modified="2019-11-13" version="1">MSRKQLLGIMDRYKHDIVSCGTNYDRVRRAICSGYFRHAAKKDPQEGYKTLVEGTPVFIHPSSALFNRAPEWIIYHELVLTTKEYCRDVTAIEPKWLTEVAPTFFKVADAKRCLNANETRESNLCSTDLPRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEC6</accession>
    <name>A0A5B0NEC6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TM7S3/TM198-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_032830</name>
        <name evidence="10" type="ORF">PGTUg99_019612</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEC6"/>
    <dbReference type="OrthoDB" id="102260at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025256">
        <property type="entry name" value="TM7S3/TM198-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39469">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39469:SF1">
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="1"/>
            <end position="21"/>
        </location>
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            <begin position="22"/>
            <end position="764"/>
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            <begin position="160"/>
            <end position="182"/>
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    </feature>
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            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="223"/>
            <end position="241"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="253"/>
            <end position="272"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="278"/>
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            <end position="346"/>
        </location>
    </feature>
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            <end position="121"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13886"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1072338.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1086370.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="764" mass="81881" checksum="A7BF06C349B0AEC9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGL1</accession>
    <name>A0A5B0NGL1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">PGTUg99_018483</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Puccinia</taxon>
        </lineage>
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    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </lineage>
    </organism>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
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        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
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    </dbReference>
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        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
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        <location>
            <begin position="1303"/>
            <end position="1327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
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            <dbReference type="EMBL" id="KAA1088571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1346" mass="148166" checksum="38A8469A4333AA3B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIL5</accession>
    <name>A0A5B0NIL5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_006219</name>
    </gene>
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            <taxon>Basidiomycota</taxon>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2506010at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="729" mass="81838" checksum="CBDADB7A9E033130" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLP8</accession>
    <name>A0A5B0NLP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLP8"/>
    <dbReference type="OrthoDB" id="2507611at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="406" mass="45892" checksum="7B3712B2A26922C5" modified="2019-11-13" version="1">MDIRVLCISIERVSAGGDHRHSNRTLEYKNPADLLPPTKKPKNQKQPKTNQKPRNNNMQSSSVRFLPPSAEAKTHHRFPSISNINPENLYKERLSPIPRPSSTHLDNLPQRYPTLSHDSSSRHTNHRNPIDHTHLHPSHHHHHHPHSTHHHQQYHHHQDRHLSSEKPRPSSISKIPNHPPPSQQQQALRPSISRTRTQSLTEASLKPTSSSSSSITAHQRSWIVPLNSLHPRPPASRTPSPSPGSSALAKNSITSIKKNLVEILDRFACPLEDKLLLVAKLHAEVEAELTQLSELSVGPTYTTHNSRSSRHAHHESVDRSANHKKGQTQTGQQNHSRKEIEDMVRLIKHADRIKAHALQQLKQDLSNSHSQSDPHPDPYTRPLHPPSVHTSNNSGRVALLNRTDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNS0</accession>
    <name>A0A5B0NNS0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_003415</name>
        <name evidence="11" type="ORF">PGTUg99_037058</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2123952at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15486">
        <property type="entry name" value="ZIP_Sip4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051615">
        <property type="entry name" value="Transcr_Regulatory_Elem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313:SF78">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="292"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="217"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="394"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="447"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="716"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1033"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1201"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1323"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1587"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="117"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="501"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="716"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1234"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1251"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1594"/>
            <end position="1609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1070205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1090196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1625" mass="180340" checksum="0DEAE0DED1CE8624" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0C5</accession>
    <name>A0A5B0P0C5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription initiation factor TFIID subunit 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TAF9_4</name>
        <name evidence="7" type="synonym">TAF9_3</name>
        <name evidence="8" type="ORF">PGT21_034605</name>
        <name evidence="7" type="ORF">PGTUg99_022658</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0C5"/>
    <dbReference type="OMA" id="WNMKDEW"/>
    <dbReference type="OrthoDB" id="341924at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07979">
        <property type="entry name" value="HFD_TAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003162">
        <property type="entry name" value="TFIID-31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051431">
        <property type="entry name" value="TFIID_subunit_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068">
        <property type="entry name" value="TAF9 RNA POLYMERASE II, TATA BOX-BINDING PROTEIN (TBP)-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068:SF4">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02291">
        <property type="entry name" value="TFIID-31kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="174"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1065318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1095017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="222" mass="23982" checksum="E3514F8FAF86989A" modified="2019-11-13" version="1">MSTPANNPNAEGATSSAPEPSIRQLPVPSTPRDARIIALLLASMGVQSASEGVVRVLMEFAHRYTVDILSDALVYAEHAGRAHAAHGPDVEDVRLAVQAKVEHSMSGPMSKEFLLNLADNLNRTALPPIPERYGIRLPPEKDRLTAPNFDIAPKPPPTEVESPSDHEEGMFGGIDDDDSSDSDSDSDTEMDFAPVISINGNGNLASDQARRRLEDVEEDDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0J9</accession>
    <name>A0A5B0P0J9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_002021</name>
        <name evidence="7" type="ORF">PGTUg99_009108</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000115">
        <property type="protein sequence ID" value="KAA1129996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0J9"/>
    <dbReference type="OrthoDB" id="25131at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039328">
        <property type="entry name" value="WDR89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366324" evidence="5">
        <location>
            <begin position="19"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="294"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="348"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1093890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1129996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="384" mass="42334" checksum="FE27C484C86337C7" modified="2019-11-13" version="1">MAYILQLVPLLTSTGTNCCASASSDQADSIRIFDLQRTDKPTIELEYSEQLGRLSQLKSHQHSLLIGCFSSSPAVLGWDLRSADHRPSLELTGHSSTPFLSLGHSAGSVIAAGTSNDDDCSSAQIELFDLRRGTKPYCLYDQAHSDSITSLEFNPSCSDHQLLSASTDGLLVLHDTRIVDQEESILFTSNTGASLAHARWQPDGRTIWAGSDMETLSRWDAEQLSLLKDYGDLRQDSLAKPDPSWHEPVSYLISLATPSPTQSAYFAGSQSGDVVLVETTDPETERWKVLGSFKGGHSEMVRCATLDSQNSVFLTGGEDGRICIWSNDRENEASFRLVDRFNPFSTPIIHSPKEPNPTHLQLKPDSSLKTLSSKDNRFKPYNRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2G8</accession>
    <name>A0A5B0P2G8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014538</name>
        <name evidence="1" type="ORF">PGTUg99_022478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2G8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="110" mass="12758" checksum="5A03E5BEB9555DB5" modified="2019-11-13" version="1">MKASEISEIETVGHSGQATLSGQNLAEEKLPTRISSANYDFNNKKDVRLYWNSQFKILKSSNTKRDFESFKTKLKNKKRLQIERLSDQISRHFINIEISEILHDIRCGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P308</accession>
    <name>A0A5B0P308_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_036697</name>
        <name evidence="6" type="ORF">PGTUg99_029369</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P308"/>
    <dbReference type="OrthoDB" id="1936594at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="549"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="583"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="635"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="662"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1095222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1121490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="918" mass="104515" checksum="86DB6E8584C0C5FE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7I1</accession>
    <name>A0A5B0P7I1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7I1"/>
    <dbReference type="OrthoDB" id="2504216at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="52" mass="5850" checksum="F90954140D43C9D3" modified="2019-11-13" version="1">MATEVIRESKILLSNENYTLWLLPIEAKLYKLKALNIVTGAACQPQSTFHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8J3</accession>
    <name>A0A5B0P8J3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fungal-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004247</name>
        <name evidence="3" type="ORF">PGTUg99_034593</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8J3"/>
    <dbReference type="OrthoDB" id="2747778at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040976">
        <property type="entry name" value="Pkinase_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248:SF2">
        <property type="entry name" value="FUNK1 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248">
        <property type="entry name" value="FUNK1 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17667">
        <property type="entry name" value="Pkinase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal-type protein kinase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="107" mass="12268" checksum="AE3F4F100648BD44" modified="2019-11-13" version="1">MDCQKVEITRQSDQLGEPKNAFINRTQYRRLILKDAGEPVWKVKTPVRLLEAIEHCIQGHKDLLDAGYLRRDISINNLMIIDQADDPDRRSLLIDLDCAIPLSLSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9B2</accession>
    <name>A0A5B0P9B2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9B2"/>
    <dbReference type="OrthoDB" id="10549371at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="116" mass="12692" checksum="3BFD5A7FF6AA49A3" modified="2019-11-13" version="1">MDQPHAKKRTFDHSPLAEPIDRSISTDCTTKKKNKEGKKNLPPRREIDHQTRWLALATNGGPTSLVPRCRCATPKQRGSRGGSKGFTQPPSASNPLPPSASDQARTNHEGPVISCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9R8</accession>
    <name>A0A5B0P9R8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_033214</name>
        <name evidence="10" type="ORF">PGTUg99_001546</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000176">
        <property type="protein sequence ID" value="KAA1125589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9R8"/>
    <dbReference type="OrthoDB" id="10252718at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013870">
        <property type="entry name" value="Ribosomal_mL54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L54, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08561">
        <property type="entry name" value="Ribosomal_L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1098317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1125589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="125" mass="13985" checksum="8ADA57668287C0BD" modified="2019-11-13" version="1">MEVSRDLGYFRHSESKQVCSRAASILGVRHLASQPTQEKKTSQAKKTVPVSTIPAGSPIKGLAYIKGESDPIAKADDEYPSWLWTLLEPNMGVGHADTPLRAIRRELNRENRNNIRKSNFLKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAX0</accession>
    <name>A0A5B0PAX0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAX0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="79" mass="8674" checksum="F107298A990E207F" modified="2019-11-13" version="1">MSPGYDAHLRLNSSKSARSTPTTPSPYATLGLFSDLVGQRLHFSNLDEVTSLTRSNFVAFNLCSPLVDNTETISLWQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBL8</accession>
    <name>A0A5B0PBL8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026563</name>
        <name evidence="2" type="ORF">PGTUg99_034268</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBL8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="69" mass="7660" checksum="67EFC0B87F6D5FDC" modified="2019-11-13" version="1">MGNQSCVVVMDALVDDRKPFTVDQRPGTGAAWLIHHPQRYRASPSHFLLAPSLSGRQPQLSDHGNIRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCS1</accession>
    <name>A0A5B0PCS1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005638</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCS1"/>
    <dbReference type="OrthoDB" id="2507740at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022950371" evidence="2">
        <location>
            <begin position="21"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="473" mass="54001" checksum="ECC16DDC5A04BDB5" modified="2019-11-13" version="1">MGIMTFILLGAAQCMELGRAASSGRLAEMPEASDRAGPLIGSSEEAKMSKSYRDPFKDRSSVKPENHNLEASVGDIDKMFQLRQDSLENFQKSITKSMKTRDGGEAVGIYDDFANELRFSAGELHSLREDIFKISKIRDRKDNMNLVIRLENILGHTPPANFPMRQGITAIEKEALQVQARELEAMIFQTIDFMHDKNLISPVERGAFFQREPAKHRFALHMMMDSKGQDSDVYVRLHTKGGLTPANQASEHLSKPTTANMFEKAPHPYYSLGPDGITKHFHFEDVLASRKGVYFSLHSDLFRSTETDSLYLMDKAVDIDTQFEGTRNDLQKLLSQILQSSKEETSEPASYYGQMTRRFRENAEELNSIHEKILQSASQLHGENLDQLNRLKTTLNELPLLKDIGDQFNRKEMIKLRHYAQELQASIFQTVDFMAQKKMITLDEFQGFFRDENAMKHLASHLVLSLMLQFFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEM3</accession>
    <name>A0A5B0PEM3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_017725</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEM3"/>
    <dbReference type="OrthoDB" id="2505994at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022794001" description="hAT-like transposase RNase-H fold domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="317"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1099711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="47805" checksum="3606F6E35B1B032A" modified="2019-11-13" version="1">MHIRCFCHKIALIVNAGLAKLGMIAPPPSKVKESILGTFPYSDVMETIAEVEEEMEEDNNDQANGPDHQTNPSIDDDEISEPEHNEEELARLGDQQDKEEEDNSPGEYQAANRNESNRLDHVTRKLDFVVRKITSTSAWRQVFIRKTNKKGLKLRSLIPGYGIRWNIKFESRERAYEAREVIDEILKEDLEKVHAQRARQRGRTSQKELGHFQEMTILSHEWQEIKELNDELKKMEGDGPTGSAAIPEYLQMKNTLKKKLDSSNRNDPLHPMFVKMFEKTNNYLLEALACETLVISTILNPSFHLAIFEKHFPKEALDAKKRLVELFEERKNQMAEKIQQEKEREKDTALKEKETNEDDIYSFFSGPSNNSGQDEIEMYLGGVDRIIHGNKKDYMFSALKWWKVCFLLASSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFN2</accession>
    <name>A0A5B0PFN2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007675</name>
        <name evidence="2" type="ORF">PGT21_007918</name>
        <name evidence="4" type="ORF">PGTUg99_010148</name>
        <name evidence="5" type="ORF">PGTUg99_016081</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000243">
        <property type="protein sequence ID" value="KAA1120514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000210">
        <property type="protein sequence ID" value="KAA1123251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFN2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1120514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1123251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="224" mass="24927" checksum="15B21BE9B759D4CE" modified="2019-11-13" version="1">MNLHLKKSPRPTYIPLPQQGSHVSVVVHTQLENRNGVVKRSKSLGLRRVSRDSLPKSRSLAGTPTTPTRKLAKMQNAIFEVLKEREPATVIHPKPLQSRFQACLSSLVATLDACADTGVCKYANRFTVINPCILNELPRILAQTHARVLRGLHPSAVAWADPFVGSFMYSLEAQSSNLVQQQCRPSSARRRHTYPGTICTRSNVIPVICHWRSATDDRNTSSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHP1</accession>
    <name>A0A5B0PHP1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008543</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHP1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="9246" checksum="6FDB6E8EA92F960C" modified="2019-11-13" version="1">MAPSSIKLKPFMHSAFVFRLLILFQNVQRPSRSEFRRRTCYALARSRHKRTLKTDANGLQMCVRQRATHHVRESLQAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLQ0</accession>
    <name>A0A5B0PLQ0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial inner membrane protease subunit</fullName>
            <ecNumber evidence="8">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_034069</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S26 family. IMP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="8"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLQ0"/>
    <dbReference type="OrthoDB" id="308440at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052064">
        <property type="entry name" value="Mito_IMP1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019757">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Lys-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383:SF16">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383">
        <property type="entry name" value="PROTEASE FAMILY S26 MITOCHONDRIAL INNER MEMBRANE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00760">
        <property type="entry name" value="SPASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S26" evidence="9">
        <location>
            <begin position="38"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1101946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="242" mass="26784" checksum="781DD517CE5A8D75" modified="2019-11-13" version="1">MNSASTFKTTLHLVKRAVQVTAGVSLFKDKVGDLRWCEGGSMLPTLNVTGDLLLQIPLSSVLNYSLRGDRARTAKKTAEAEPTTSRLNLNRGDLVNFVSPSNPSVLACKRIIGLPGDQILVDDLPHHFNQITADSILHPELDYQYSHKSLLTIPQGHLWLQGDNYAVSIDSRTYGPVPIGLVSGKIVARVSISFCSLFLPFFSAFLLFFLPFLTRVNALVSFWHLDDIVENICNDKPILICF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNP7</accession>
    <name>A0A5B0PNP7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_024211</name>
        <name evidence="3" type="ORF">PGTUg99_006964</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000338">
        <property type="protein sequence ID" value="KAA1102240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNP7"/>
    <dbReference type="OMA" id="HFKLSVA"/>
    <dbReference type="OrthoDB" id="10310277at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1114811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="116" mass="13146" checksum="E70294D1C4E4189E" modified="2019-11-13" version="1">MSPIIINQIVNLENLKLTFVRPQAFYQTSPDKSPAPLITAKTVVILIDIIIVAIISILILMGYFLYRRQLRRKENLTKSFETSEAKMASESNHPAMDSHSICSSTTETKEESIYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSB0</accession>
    <name>A0A5B0PSB0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSB0"/>
    <dbReference type="OrthoDB" id="10564449at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="182" mass="20154" checksum="43E94DFCD306FB22" modified="2019-11-13" version="1">MLVAFLLSILPPNAPQIVLLHKLQPPTPTISSVKYHVYPGTVKSFFLAVELFFPGQHLPAQEVTRWNTELSTGIPWSIESVEKSIINISLILNNLPQLISSHPRLPEEATRSLWIDLDHTHQLCVSEEILKTAGNLSLITPDMVNMDQVSQPRKASAQAGSHILAPNLATFFAPPKVAYTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTL1</accession>
    <name>A0A5B0PTL1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTL1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022990976" evidence="1">
        <location>
            <begin position="23"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="236" mass="27550" checksum="748DB5BF75716408" modified="2019-11-13" version="1">MHSSYSQFFIFFPLLLLKNSTTLPMYSDFDLERVPLLDANPGEHIDSIKESYLGKIHLLEDQADPLNRNHSNVSVMEERNKVDRAIVLQRASEILDKSIKKPPKDLEHDSFKQIHIDPSKGAFWRMESHSDLMEVIPEIFLFRATRYVEYGRSARAKTDKIINNPKLPKEFYTIVNKVDKLVEKVTSRYLSFPMIHLQYLNLHSYFCHALQDQFSNDDEIFDLELNPGFVDLSSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYP0</accession>
    <name>A0A5B0PYP0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYP0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="73" mass="8018" checksum="F7945F8D72E98BAB" modified="2019-11-13" version="1">MIDRTTPVELEGNMPSRSLTASTPANKKDTKISEVRSSEGKGHVTPQPHRLALLHLFPHLKFSILNSPSSSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q019</accession>
    <name>A0A5B0Q019_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_036103</name>
        <name evidence="7" type="ORF">PGTUg99_020403</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q019"/>
    <dbReference type="OrthoDB" id="539541at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="5">
        <location>
            <begin position="246"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1106551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1126252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="363" mass="40558" checksum="6A351D0396D88F55" modified="2019-11-13" version="1">MSLRSFLPTRLVLNPGCKSIQCLRRQGPSEWTTKRSIQSTSQTRATVVSEPEGERTTEMQRVKKDSSQTAGLFSIRPGQLYRSRLAEHYHNQMASDLLYMTYELPAGYALDGSTPHRADGRALTQHPDPISKLPQWDPEDPYTRNRPPRPPKGGSGSLPRRPPSDSSPENLVRLKKISIDIHVPEAIHTKKELLGAIYLLQLISGQPDRAALSTDQLSKLFRSEGIQISRTKDKSTTFKVRTGMVCGAHLEIRGPKMFDFLDILSTFVLPRLKDFNGFALPPVDAHRRHAAMVSGVVQIGLPDHAMGLWPAVEESLENWPRKYGMNIHFLTNATGPGASDKARALISGFRVPFVRPQDAKLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0U3</accession>
    <name>A0A5B0Q0U3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036864</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0U3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1106659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="165" mass="17962" checksum="858C136F9FEDB658" modified="2019-11-13" version="1">MPVPVSIPIKTADIFDRVRLPTSSSGFDRIFRASTTNRVIFLPSAESIKVIGQDQSASGCSASRRLIGAVSIGPAHININYRHCQIPRPTPASAGVAGRVALRSRFRGSVLIPPQEVPEPVEAFRIKSHVCPTIAPPQEVPEPVEAFRIKSHRSIECEASNKNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2N9</accession>
    <name>A0A5B0Q2N9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent DNA helicase chl1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CHL1_1</name>
        <name evidence="2" type="ORF">PGT21_005000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2N9"/>
    <dbReference type="OrthoDB" id="267079at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0034085">
        <property type="term" value="P:establishment of sister chromatid cohesion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004187">
        <property type="entry name" value="RAD3-like DNA-binding helicase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="ATP-dep_Helicase_C"/>
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        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF41">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE DDX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent helicase C-terminal" evidence="1">
        <location>
            <begin position="123"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="319" mass="34828" checksum="ECBA56743687EA00" modified="2019-11-13" version="1">MQAFILAMSNADKDGRILMVSERSTASEQPTITIKYQLLDASSSFSDIVSEARSVVLAGGTMAPIVPPEHLLVRAVSKGPMGTNLQLKFTSKDDPKMQDDLGQSVANICNIIKDGVICFFPSYASLDTLTDRWKKTGLWTRLENKKKIFIEPKSAADVDKILNGYSAAVNQSTNPTSVSGGGLMLAVVGGKLSEGINFSNNLCRAVIVIGIPYPNSQSVELKERIKYVENLKGAQKDAGKNFYANLAFKAVNQSIGRAIRHSKDWSAIILLDSRYGAPENVNRLPDWIKAGFKTTEGFGSLIKDLAQFYNQRRRDGLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7K5</accession>
    <name>A0A5B0Q7K5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_97</name>
        <name evidence="2" type="ORF">PGT21_033829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="238"/>
        </location>
    </feature>
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            <end position="1627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="61"/>
        </location>
    </feature>
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        </location>
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    </feature>
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        </location>
    </feature>
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    </feature>
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    </feature>
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        <location>
            <begin position="852"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="871"/>
            <end position="881"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="1048"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1576"/>
            <end position="1621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1627" mass="177016" checksum="43C6EEAC5B872733" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q8V9</accession>
    <name>A0A5B0Q8V9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GTPase-activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GYP1_2</name>
        <name evidence="1" type="ORF">PGT21_033513</name>
        <name evidence="2" type="ORF">PGTUg99_031924</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="26371at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1106339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="90" mass="10334" checksum="53129E0AA0BDF5B9" modified="2019-11-13" version="1">MPFFKVFLTAYINGEEAELFDVGRLPAEALEAIEADSFWSLSKLLEGIQDNYIFAQPGIQRQLARMKELCERVDVKSYTTNTRDDVLMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9Y0</accession>
    <name>A0A5B0Q9Y0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clr5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="5392716at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21711" checksum="F5158BC03BA33E65" modified="2019-11-13" version="1">MSEVYTSDYSTEFTGSDDTDHDEPTSSQNPQPTDMEQVVVRYLLSQGYKGPQILVILEERHGIKMSIRTLNRKRKLWGLQQRDLPRGPPPAIQASIRSSHSKGLILKEIQARLYKETKLDVSIRTIQRYLQQLNLRQQTNDLQDGKVSWDKVIECINHARTELLQTSAGYRSMHRILKRFYSISIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA90</accession>
    <name>A0A5B0QA90_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alginate lyase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009953</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QA90"/>
    <dbReference type="OMA" id="HAYDEHE"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1069" mass="120685" checksum="7318444FEF37A844" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAQ9</accession>
    <name>A0A5B0QAQ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAQ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="149" mass="16491" checksum="3AB6078C9EA5663F" modified="2019-11-13" version="1">MTRSTNYKYSGRRWVQADQGRPEFPRMTCHSSGNGRSNPIGVLPVYTAGGLDHHSEHMFLSTMTGALSSVTSSTYRRAVNPVPRGLLPRPQARMIRRAMQGWPSPPAVPAGLLPAFPPTNYFTSPICRKHLIGSDPHQLMRPETESSGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBE6</accession>
    <name>A0A5B0QBE6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBE6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="109" mass="11716" checksum="C917F0286BBADC00" modified="2019-11-13" version="1">MSEISLTINLLIPITNCSQPLSHIINLQHHTHLLTISSPISTTQLSNTRFNQTHHQSISNHQPLSDTGLNSPSISKTALNSSTSSKTGLNSSPISNTGLINSKHQPSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCD9</accession>
    <name>A0A5B0QCD9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCD9"/>
    <dbReference type="OrthoDB" id="2506001at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="49055" checksum="87807E25CFDDA2B0" modified="2019-11-13" version="1">MSTNPQSHKSASVSGHPDSEVSDSNAGPGYATNQSNTQLRRSSRASSLVTAPNMVTPASDSRVRLNRPATAQVQKHHPEASSDQEYIQGAAPSKAKAKSKNKRQRRKPPSPADHTSNRVTQPKKRKAGKSASNTVEVISNDETYNYDQDSDSVSILNPKRLGEKSAKGDGFAHPLDYFHPPTWKEGDPHGTKLNFKCRWCDKTYRGQLLSNGNLKTHRDGSTQADKNPKGCLNREQAKKAGIILPLSVAKTRALQDNDQVGSNLKLQFKPTFVNRVLNQIIMMWQIRQALPWTRIEDPYLRAAFLFANPKAVLYGRRWSADESKNLYTVLKSHVFEELNNLDTKFTLIHDVWTTKGNRFAFIGAAVAYVDQNWEYVVWHLGLKMIPWKHRGYLLARPIVTILKKNNLHHKRLRVKQQYDGECHVFIAQQCWRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCN9</accession>
    <name>A0A5B0QCN9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WDR59/RTC1-like RING zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_035801</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311712at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16488">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="203"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="WDR59/RTC1-like RING zinc finger" evidence="5">
        <location>
            <begin position="1198"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="380"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="506"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="655"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="896"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="967"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1011"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="380"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="514"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="896"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="967"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1011"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1029"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1041"/>
            <end position="1051"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1111020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1311" mass="145574" checksum="48C1691E6C2EF0A9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHT7</accession>
    <name>A0A5B0QHT7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_009451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHT7"/>
    <dbReference type="OrthoDB" id="783096at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440:SF0">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="3">
        <location>
            <begin position="345"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="553"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1112778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="738" mass="82495" checksum="A38900AB147D6A77" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QLZ7</accession>
    <name>A0A5B0QLZ7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_002174</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QLZ7"/>
    <dbReference type="OrthoDB" id="543373at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1114257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="37261" checksum="B366AEA7B15FCC81" modified="2019-11-13" version="1">MFSWQELSREGGISLPRRRDLAVDEWSTVLRPILLARLLPQQQQPPTPSNPILQTALLDIFVALPWLFSSSPVELESIVLSSLSSDHFPLRLAALQAICVLTLDLETSFAQPVLQAILLPLPSHPNQPDLETALTYLIEFATFNDIPDLTTWIEPLLQLTLDQNRIISTRSTALECLITLIESRSPAYNLAPSLNWLNPVFRALVNLMAEIDEDEHWSSSLDGDEEDQDAIYIQAEQALDRLTQEVAKTHCDQLFELILSAVQPPLPASWKAKHALLSVIAVTGDGLAESFSLATEQIYHVLQAGFDDPHPRVIYAAIYAIAQLASPLKVRDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN45</accession>
    <name>A0A5B0QN45_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA dimethylallyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_009045</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN45"/>
    <dbReference type="OrthoDB" id="775260at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004202">
        <property type="entry name" value="tRNA isopentenyltransferase, putative (AFU_orthologue AFUA_6G05070)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF89">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="398"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1114445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="49359" checksum="06CD01CFD18876E3" modified="2019-11-13" version="1">MTKKDIHATKSPDFNQSSILDDSEEILERCRQNGFDPNLLASLKSISLDHLRLVFLLPHLPPFSSPKEFPPDFPIELLPPKYQPPQATAEDLCIGLHEALQSIDPVMAGRWHWRDLRKVRRSLEVALCSGKLMSNLVAEQDQIDNRASESQEERIPYRTLVFWLYSETEQLLPRLDARVDKMIENGLMNEVRDLKIMRGRLTEGNQTRTDFSRGPFQAIGYREFESLLSTTDADSEPEPLQSKKLAEATELTKIATRQYAKSQVKWMKNKFLPQLNKFGSSSETLDPPNNAESHMMTYILDASRVDEWDQNVLIPAQNILSAFLNYNHLPSPKSISSIANSILSESQVKKTRSLKELQLKVCKTCTTEKHKPFLIEEVNWEAHLRSKKHRRSLTIIERNSKSPAIESNVHHPSSQPSSSTAQSDEKSSRSSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN46</accession>
    <name>A0A5B0QN46_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN46"/>
    <dbReference type="OrthoDB" id="5564877at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39473">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39473:SF1">
        <property type="entry name" value="DINB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="279" mass="30405" checksum="368FF02F1486DBAB" modified="2019-11-13" version="1">MGEVTAEDRGASGSRTSNTPDIIGDPSTRHSGSITRSPSSAVEQYKLLRSVAIGLIDQAIDLLHHVVVSDGLLSSVSELVPGSTIGKHLRHLHDHFRILFQCALSGQPGDHKVLNYDTRERNVPMESSHKTILEEFERLKHRYLGDPESPLNVEPNAAGPFCEDNPDPPVLDRASSPSAHPSTPSSSSSSSSSSSLQSLMSGKNGISRPRFDHDMALDLVAITPHRVGLKTTFNRELWFACLHATHHYALIRVIVTGELKLSLPKEFGVAPSTLERSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXR2</accession>
    <name>A0A5B0QXR2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pre-rRNA processing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RRP9_2</name>
        <name evidence="7" type="ORF">PGT21_031331</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXR2"/>
    <dbReference type="OrthoDB" id="189968at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000899">
        <property type="entry name" value="Chromosome 15, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039241">
        <property type="entry name" value="Rrp9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="267"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="312"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="354"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="491"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="407"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1118101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="608" mass="67792" checksum="7085C477D6DAA70F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZL4</accession>
    <name>A0A5B0QZL4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZL4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="161" mass="17558" checksum="A91659AC755C01F2" modified="2019-11-13" version="1">MVCIRPAICPSCLSLPVSFIFPAANPFPERPDRNTYLSSSGAVNRRLRVDFPIRNPRESPIQEGKSHWCSEPTTSTHTLGTFSSSAFPSSKAAPASQDRKTIYEEMDVIKILEYFYPSTDLDQKQRSFSPSSSSPSIARSTPAHPNPSTGTSSSSGKSLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0L6</accession>
    <name>A0A5B0R0L6_PUCGR</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0L6"/>
    <dbReference type="OrthoDB" id="2504265at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="520"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="536" mass="59469" checksum="2DB580449FCB18AE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R300</accession>
    <name>A0A5B0R300_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RED-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_034131</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R300"/>
    <dbReference type="OMA" id="LKSTHMV"/>
    <dbReference type="OrthoDB" id="3366823at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Red-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012916">
        <property type="entry name" value="RED_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12765">
        <property type="entry name" value="RED PROTEIN IK FACTOR CYTOKINE IK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07808">
        <property type="entry name" value="RED_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RED-like N-terminal" evidence="4">
        <location>
            <begin position="75"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="233"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="65"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
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            <begin position="238"/>
            <end position="249"/>
        </location>
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            <end position="262"/>
        </location>
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        <location>
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            <end position="299"/>
        </location>
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        <location>
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            <end position="311"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
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            <end position="412"/>
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            <end position="436"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="3">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
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            <begin position="499"/>
            <end position="522"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1119789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="539" mass="59532" checksum="68CDD4D5A6462834" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3J7</accession>
    <name>A0A5B0R3J7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036726</name>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3J7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="10740" checksum="7A31AD20119B9255" modified="2019-11-13" version="1">MDNRPESFTPWVPVDATLSQHEMLDLSAGVLTLLSTERTSGPLCVVGPSGYPEEEQLDAARSASDPIAIPSNASSPAFFQSYCGGDGYWELVHGDNDDPGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3W3</accession>
    <name>A0A5B0R3W3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_037153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3W3"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF89">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="615" mass="70071" checksum="14CBB0F86E157D76" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R713</accession>
    <name>A0A5B0R713_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022968</name>
        <name evidence="2" type="ORF">PGTUg99_029021</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1121049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R713"/>
    <dbReference type="OrthoDB" id="2500038at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1121049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15356" checksum="30D812240E44DF11" modified="2019-11-13" version="1">MLLNIEGTTSLLAQVVTPGTFLAALITLDGRVVAYHIHPSAIVADEDDEQSDGSDQAKIAAAIASGLWREDCYDRPLSSNSKTELANEVRNLDAVCELGQLRLNFTPPFLLVLVGKTGSVSTETLSMKAQLLQDQLKGPFSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R7H5</accession>
    <name>A0A5B0R7H5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050219</name>
        <name evidence="3" type="ORF">PGTUg99_050179</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R7H5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="70125" checksum="A904FE26E12881D8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RFV7</accession>
    <name>A0A5B0RFV7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013113</name>
        <name evidence="2" type="ORF">PGT21_014418</name>
        <name evidence="3" type="ORF">PGTUg99_034507</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RFV7"/>
    <dbReference type="OrthoDB" id="2511548at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="93" mass="9965" checksum="EC4B6919A4051EAE" modified="2019-11-13" version="1">MLGPERAVDAGPSSWTFVALEEAEEISDPSTVPDPSLSALAVLASPQISLDATTDLVVRKRHRPDNDPSSDLTGIPKRPWIIFESVGALDTHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJJ1</accession>
    <name>A0A5B0RJJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019396</name>
        <name evidence="3" type="ORF">PGTUg99_009102</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000173">
        <property type="protein sequence ID" value="KAA1125996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJJ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="134" mass="14913" checksum="A425166110D7583B" modified="2019-11-13" version="1">MKQDLYHLWGLNSISEKQQEHKPLFLQNIGPRNPSNTLICFDGASVDGNDGRKIKYEPSSLVKDPEVFDNEANYIPASKKRKLDLDLSLGLPNEDAESSQLGQASHQSQKSHPSCKPTYSNTSNLSVPNVTNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RPG1</accession>
    <name>A0A5B0RPG1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_002521</name>
        <name evidence="5" type="ORF">PGTUg99_002523</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000147">
        <property type="protein sequence ID" value="KAA1127881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RPG1"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF6">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474824" description="SET domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="3">
        <location>
            <begin position="618"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1107110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1127881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="907" mass="102646" checksum="89029D5F20DBD3F4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RSC5</accession>
    <name>A0A5B0RSC5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_027941</name>
        <name evidence="8" type="ORF">PGTUg99_003102</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1128916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RSC5"/>
    <dbReference type="OrthoDB" id="2497484at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02181">
        <property type="entry name" value="GH16_fungal_Lam16A_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000114">
        <property type="entry name" value="Probable endo-1,3(4)-beta-glucanase NFIA_089530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF24">
        <property type="entry name" value="GLYCOSIDASE C21B10.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26113">
        <property type="entry name" value="GH16_XgeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474853" description="GH16 domain-containing protein" evidence="5">
        <location>
            <begin position="27"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="6">
        <location>
            <begin position="109"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1094670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1128916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="423" mass="46483" checksum="30B92385E04F70D0" modified="2019-11-13" version="1">MSRTKVLSTAFLIGCFLLLDLSYTSAECAPSSSPHHHRPHRGGKHPKLRDQVDNGDYGSKPSYIRKQDDDGDYGSKPSYLRAQDDNGDYGSGSYNPNSNLLGLDLPIQLGYGREPNSRGKKYNLTLSSSGNKFFDDWDFFSDSDPTHGQVAYQPVNEAWKQGLVSVGPSSHDKNLITAKMKVDNTNWLAQGEARKSVRLSSKKSFKYGLLVLDALKMPFGCSTWPAFWTVGNNWPHDGEIDIVEGVNMLNTNQMTLHTGPGCTIQNPMLQAAVGNVLNPDCDVYASSNLGCGVHDHSNASYGQPFNQAGGGVFAMEWSPNGVSIWRFSRGEVPRDLQSGHAPQPSTWPIRPVAHWSSDICNNMNDEFSEHRIIFDITLCGDWAGSAGVFNANNACSGSCTDLVKDPTNYKDANWEIASVKLYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S102</accession>
    <name>A0A5B0S102_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012260</name>
        <name evidence="3" type="ORF">PGTUg99_020449</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S102"/>
    <dbReference type="OrthoDB" id="2499834at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="493" mass="54261" checksum="9A3DE7FC956EC554" modified="2019-11-13" version="1">MPPCSVVHILPPQYQDNFNRPILDTRSPIPSLNQHAAILQTGTTAPIIDHLENSASQTPQLDVQVPAPPPSNSLTQPPSKKRKHTVESNESMLPQPIDILLKKSYNELVTHAQENSKGSMSQADRDFFLEFYEEQRKLLAIKAIEHQLSMPMVDAFLGKRLALKGPNCWNQFLKTPEARAIFKETGGVDDQSAMKKVSELWHKLTPEEKKGFANKIDDGLTDEERALDDDNSEELIVPVEPAAPSTSGKDSVPTSGQLSVRTEGSFRHDYFTVQNFVNDFVAKATHISATHNSQFVVFAVSTHLGPSSYQISQCTPGANVFLTYSKDVDAENHYAARFQSHITGYNVAQIAELADKTPKKNLKKPSHEVKVTARMTSLIKAKTEGVVKRWPWTDTVSRLAEIGFEIALAPSARINFDWITQPSRQLKVEHIDSLHLDLDDQLIDILRIPHNPPGGTHRASPRGLGGGGPTGNVNGSLSQAGNPTNTNEGGADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S409</accession>
    <name>A0A5B0S409_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022212</name>
        <name evidence="3" type="ORF">PGTUg99_029127</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S409"/>
    <dbReference type="OrthoDB" id="2508388at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="704" mass="80684" checksum="5A177C5EB309A733" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S7T5</accession>
    <name>A0A5B0S7T5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM2-LSM8 complex subunit LSM8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM8</name>
        <name evidence="13" type="ORF">PGT21_008023</name>
        <name evidence="11" type="ORF">PGT21_009793</name>
        <name evidence="14" type="ORF">PGTUg99_019723</name>
        <name evidence="12" type="ORF">PGTUg99_023913</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000439">
        <property type="protein sequence ID" value="KAA1082152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S7T5"/>
    <dbReference type="OrthoDB" id="10263346at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01727">
        <property type="entry name" value="LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000027">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034103">
        <property type="entry name" value="Lsm8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF9">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1079384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1082152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1082600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1133505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="96" mass="10790" checksum="C9C398EFCB3B475C" modified="2019-11-13" version="1">MSSKIAEYLDKRVLVITQDGRTITGELKGFDQTTNIILSDSVERVYSSDEPMEEVPLGLYIVRGDHISVIGELDVEADAKIDWSSIRAEPLDEIRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SAE8</accession>
    <name>A0A5B0SAE8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021334</name>
        <name evidence="3" type="ORF">PGTUg99_005240</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000041">
        <property type="protein sequence ID" value="KAA1135086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SAE8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138324" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15478" checksum="490B7437193974E6" modified="2019-11-13" version="1">MRAIILTSILGVNLLHIPPGFATMCYLLTCNAEGVPLKEHVIDSHQLPRQAACGRYTVMGEWKECKNLRQKEYFHCETCNTIWRENVGTVALRVCSGVECNKDHYTKEKIDIGNSPPTLQNYLGNVGGGESSAPGPSSRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SBA9</accession>
    <name>A0A5B0SBA9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_020727</name>
        <name evidence="6" type="ORF">PGTUg99_009449</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000041">
        <property type="protein sequence ID" value="KAA1135097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SBA9"/>
    <dbReference type="OrthoDB" id="14252at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000077">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000062">
        <property type="entry name" value="Vacuolar membrane protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF77">
        <property type="entry name" value="FI19380P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366728" description="FAS1 domain-containing protein" evidence="3">
        <location>
            <begin position="17"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="936"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="34"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="199"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="355"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="518"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="703"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1105819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1135097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="987" mass="109266" checksum="BF5EE9C4A0B526FD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SDP8</accession>
    <name>A0A5B0SDP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019817</name>
        <name evidence="3" type="ORF">PGTUg99_035089</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SDP8"/>
    <dbReference type="OrthoDB" id="2507258at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366732" evidence="1">
        <location>
            <begin position="22"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1136206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="418" mass="47320" checksum="161010247DEF786D" modified="2019-11-13" version="1">MVSALLPFIIIAVSFSKFSQAALPATYISEAGHGLMGFVANPVHPNPSQFDRVYIPSTAPDRAGAAPLSPPELVTIAPHPTIPMPPHPGKFYNVKPRLIFPDDPKAPPPVQDTPTPPEIAPSYHSTRPLHVVMHSLKFPPTSHDYFEAPPHSQVGSLEEYFSGRPALREVALEQQKAITEGVMTPISAENQKLWRKQSVGYLSLEPEHQAEIRFWLESHFHDGHRDPKYRTRVIAHIWSLMQSGRKNSIWWPQSLIDSADPKHNGYIDIIQAGDLLKFFDRRADFKISDQQVTEILPQLTKILYLGEDEQPRTEALDIEKLMAFKDRDNVPMYQSRVGYIMKVSLRRFFRRDQDQRERILQDLNLVVGSPPSQIIQSAKNALIKDRHSFFNLLTPKPKSAGQDDYARIEAELAKLGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SE83</accession>
    <name>A0A5B0SE83_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015262</name>
        <name evidence="2" type="ORF">PGTUg99_029032</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SE83"/>
    <dbReference type="OrthoDB" id="2505754at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1136102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="116" mass="13396" checksum="3DABA84D4C88A7DF" modified="2019-11-13" version="1">MMHKPVYIFTTVGQLMSLPAEETEEHFSRLKSNQMEDELEARNEFILILAHQMSSQLEVNDELFDDLAALRAAYASLQSSCSDYVDELEILKIRFQAVEAENEADCSRRDQSEYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SEC0</accession>
    <name>A0A5B0SEC0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Acyl-CoA desaturase</fullName>
            <ecNumber evidence="16">1.14.19.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGT21_019834</name>
        <name evidence="21" type="ORF">PGTUg99_035099</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
            <strain evidence="21 23">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>octadecanoyl-CoA + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (9Z)-octadecenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:19721"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="EC" id="1.14.19.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="16">Expected to bind 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.1" evidence="16"/>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SEC0"/>
    <dbReference type="OrthoDB" id="10260134at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000004">
        <property type="entry name" value="Acyl-CoA desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009160">
        <property type="entry name" value="Acyl-CoA_deSatase_haem/ster-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF31">
        <property type="entry name" value="DESATURASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000345">
        <property type="entry name" value="OLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0249">Electron transport</keyword>
    <keyword evidence="15 16" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8 16" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 16" id="KW-0349">Heme</keyword>
    <keyword evidence="12 16" id="KW-0408">Iron</keyword>
    <keyword evidence="4 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="19">
        <location>
            <begin position="380"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1085792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1136207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="507" mass="57754" checksum="97246A1AE576C05E" modified="2019-11-13" version="1">MSKPSPSTPATAPHLRQRQRKNLPDYDPDSDLSESEGLGGLRSQVGNTWEDDEETAVDDDSYVQRTLRKEKPLPPITWRNFYREINMISTLALTVVPILAIYGAFTTPLYRSTLAWSILYYYFTGLGITAGYHRLWAHRSYNASLPLQYFLALGGSGAVEGSIRWWARGHRAHHRYTDTDLDPYSAHKGLLWSHVGWMIVKPRRKPGVADVSDLSRNQVVRWQHRWYLPLIFGMGFFFPTLVAGLGWGDWRGGFFYAGAARLLFVHHSTFCVNSLAHWLGEAPFDDKHTPRDHIITAFVTIGEGYHNFHHEFPQDFRNAIRWYQYDPTKWFIAVAAFLGLASELKTFPDNEVRKGQYSMKLKELQRDFRDVKWPKSSNDLPIVTWEQFVEEADKKNGRDLIVVGGFIHDVTEFIDEHPGGRALIKTRLGKDATTAFHGGVYDHSNAAHNLLAMLRVGVIEGGYEVEHLKKKVGVFRKEQQIPICGPKSLGTISTPESPVVEVKPIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SHJ7</accession>
    <name>A0A5B0SHJ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022622</name>
        <name evidence="2" type="ORF">PGTUg99_005035</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000009">
        <property type="protein sequence ID" value="KAA1137317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SHJ7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1137317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="76" mass="7978" checksum="D146D72CB78985CD" modified="2019-11-13" version="1">MYSTSTSLVESISDGISFSYALKLRFFQGNGCCSSNSSAAKKIPLPICQSLKVPPSPGIPSIIYGSTAGLEKTTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LID0</accession>
    <name>A0A5B0LID0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_014789</name>
        <name evidence="3" type="ORF">PGTUg99_005311</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000515">
        <property type="protein sequence ID" value="KAA1064119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LID0"/>
    <dbReference type="OrthoDB" id="2107166at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="150"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="403"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="577"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="642"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1064119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1092814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="652" mass="70668" checksum="6ADB74B485C9BB75" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMA2</accession>
    <name>A0A5B0LMA2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024215</name>
        <name evidence="2" type="ORF">PGT21_025691</name>
        <name evidence="3" type="ORF">PGTUg99_022923</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMA2"/>
    <dbReference type="OrthoDB" id="2509777at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="132" mass="15414" checksum="A42B50389BAAF10B" modified="2019-11-13" version="1">MEYFSNTQPNSFATAHQTSLTLKTKLYLMSKSTQVLRDIQNLQLYLSKKFRPARWLDSIFEQPASTLDFNSFGRLGELDLLANYVRLIDQNYSRQLGVMWTLVEFHQYCQFQPFVKSLKSVKAMLTRIAESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQ18</accession>
    <name>A0A5B0LQ18_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030874</name>
        <name evidence="2" type="ORF">PGTUg99_029647</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQ18"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="98" mass="11194" checksum="51845F09CF996793" modified="2019-11-13" version="1">MPTVFSLRRTYRATATRRSFTHLLHSTYRAYDEDSYEARRPGIETQYAGAICPFKINCGPSSSRKQPIANEGGDYNPLYNGRVMPNQRSLVVVFDSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQG4</accession>
    <name>A0A5B0LQG4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQG4"/>
    <dbReference type="OrthoDB" id="10663496at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="217" mass="24380" checksum="401C32E26A7DFCF3" modified="2019-11-13" version="1">MTKMTRSNRPELRSPLPAPTRPRGGKGATGSGRKPPDDDLGSQREDNKNPREVEANRRPSSECGDRDAYGRGRDRIRIVGSYPDTPFNSGDPSSFQRRTDQTPGERARIALEKMSGSYSRRPYSYTQSLVDRSSGESEDSPEAYRQRGNRKLREGMEPMGQEKDSLPAPPKKQTMKFKRNEPGKRQSSSSINFADPAKWKKATELLQIAAGLYQNMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRA2</accession>
    <name>A0A5B0LRA2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021187</name>
        <name evidence="1" type="ORF">PGTUg99_003925</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRA2"/>
    <dbReference type="OrthoDB" id="2504536at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="518" mass="58965" checksum="9447B48EA9F599E7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LT68</accession>
    <name>A0A5B0LT68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000190">
        <property type="protein sequence ID" value="KAA1066858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LT68"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="8990" checksum="D681BB1CDDE363D9" modified="2019-11-13" version="1">MTSPTTDLELKAIHLETSTDLKLKLVQLEVNREFYMDLELKPFHLEVDCISKQRPRDEPAPTRGRLVPSKHCQTLNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LV55</accession>
    <name>A0A5B0LV55_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006595</name>
        <name evidence="3" type="ORF">PGTUg99_010800</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LV55"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366049" evidence="1">
        <location>
            <begin position="19"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="587" mass="68923" checksum="74460EE82861EBFA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LX82</accession>
    <name>A0A5B0LX82_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011432</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LX82"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022810016" evidence="2">
        <location>
            <begin position="25"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="226" mass="24278" checksum="9086ABBF48D54DD7" modified="2019-11-13" version="1">MFGFSAVSLVALLVSLLQVRTGSALPTPQFVPVGMPIVINPAPIVPARQVVPMDSCGDGLSLANELHPVDRRFCSETSKTRHFCRTSASLLYSTCPPLFLQEEEGAVSQIPSAPIAPTSDSPAPQAREGTCRSVEHKPNHRQHKKEPSRPTKHAQAPNRMFAFSTVTLVAIMVGLFQLQSGTASPTPQFVPGPYGPMPVGYGAAPIGRPVPVVQQFPGQPPMVRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXM7</accession>
    <name>A0A5B0LXM7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GAG-pre-integrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXM7"/>
    <dbReference type="OrthoDB" id="7691805at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18032" checksum="11799478832C65E3" modified="2019-11-13" version="1">MKGRYLGNLPSLEFHNHRSMLTTSKHLHKSIGHVNYHRLQHKLGIPVKAATVCEACAVSKITKALFHEQRSTTTKPFEEIHLDLHSAPSAQRLGSKPPLFNFIYPQPQGPAFKPQPRQQSLTPLSTTSSTTTSSSTITINTFNRYTTHSTASSRTILYHDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXU2</accession>
    <name>A0A5B0LXU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXU2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="293" mass="32472" checksum="1C2DD584706252EE" modified="2019-11-13" version="1">MCALSPPCMTPSPISFRTSPGLGEFWCSQPFPVFDTAAQSSCPRRIWTQPLQSPLRSPPRSVPSPDTTTIHSQAATIHILFFHLLVWCLGADSIFHHCCLASSRIVIALCSFDWYCSLSAMLGLFPMHRRCKQPPWVRIEHLKICAGITASSINTRFYSPFSPSPHQLTSSVFHTIANTPSPLASCYYPSSNRFYYPSTMSSIFDNCDTNDFYCEGRSDPSIEIGVRYYPDRDQWSPVFNPNVVPDDDSVGDKGPIDPAGKQGPVDVKPVHAKDKGPAEATVDPALINTIRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LY68</accession>
    <name>A0A5B0LY68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LY68"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="123" mass="13821" checksum="EEDB68FD90FB1076" modified="2019-11-13" version="1">MFMASWSDPGRLQFMASHSNPGRLHHPPTRRSIHPSKPAQLPPKTKNLPIIQSQKPPTITKMELDYLQEPSKTQESTLGPSQQTPYHGLFFEGTQPPETQIQPLEALSPPDPDDEAYKSPRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZP9</accession>
    <name>A0A5B0LZP9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZP9"/>
    <dbReference type="OrthoDB" id="10576520at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022727345" evidence="1">
        <location>
            <begin position="25"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="574" mass="67484" checksum="A5CCDEC7F05CBF0F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZW5</accession>
    <name>A0A5B0LZW5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZW5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF22">
        <property type="entry name" value="TRANSPOSON TY3-I GAG POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="680" mass="74777" checksum="531A2A03E57421F3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M062</accession>
    <name>A0A5B0M062_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mitochondrial ribosomal large subunit component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">MRPL16_2</name>
        <name evidence="2" type="synonym">MRPL16_3</name>
        <name evidence="1" type="ORF">PGT21_002919</name>
        <name evidence="2" type="ORF">PGTUg99_037182</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M062"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="81" mass="8826" checksum="755B8FEB8501D943" modified="2019-11-13" version="1">MAFAALKHAKPRLTVNLEFIHLDSKARLGGILVKKPMDTGKQIRLSSSSSSSNVLNSPTAIISKLLDQVRLSRSSSLTKHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M122</accession>
    <name>A0A5B0M122_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M122"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="1">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="197" mass="21122" checksum="B070794D893D41E4" modified="2019-11-13" version="1">MVLDVTLGTQPMLSNFGLLVKEIRALWPHASITAALGISGFNGADGKTATAQETALLAQNLDYVNLMAYDVYGAWAPTTGPLAPLYATCAPPAFGQSVQTGFQVALKQGFKASQVILGIPGYAKRLELVSSKLEEKVVNGKPTYYYQNHTTVTPPGGKFDDKPGKDICGNAQNWGGSFLVNELISNGWLTPDQKAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3S4</accession>
    <name>A0A5B0M3S4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTI2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3S4"/>
    <dbReference type="OrthoDB" id="361693at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110078">
        <property type="term" value="C:TTT Hsp90 cochaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018870">
        <property type="entry name" value="Tti2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32226">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32226:SF2">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="152" mass="16742" checksum="AF694518F711668E" modified="2019-11-13" version="1">MEPNFQPSVQQLEAILQDPKDSYTYETNDPLRTWVAILIRNALDSWYGKLSPLQIKNSAQVELGKTLIENVLAKGKRDSMVRKQATLCVGKIGKSNLNANLSNLFEDLTLHIQNVIAVAYPQPSSPALPLRPAACFQSMAVLPKPNTPAVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5W1</accession>
    <name>A0A5B0M5W1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005052</name>
        <name evidence="3" type="ORF">PGTUg99_018445</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000211">
        <property type="protein sequence ID" value="KAA1123167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5W1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366105" evidence="1">
        <location>
            <begin position="19"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="117" mass="12975" checksum="7BD2E5D560A5D31B" modified="2019-11-13" version="1">MKLSASILTLVLAGFSNAEELIQCITCKASSGVKRAEDMHRCGYSDPLMPNKPACEELREKVHIHCTIPECGQYFSMNAEPNVKTPLQCQKCDHQNSIILTPYSLTSHSEPLLNLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9F5</accession>
    <name>A0A5B0M9F5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034390</name>
        <name evidence="2" type="ORF">PGTUg99_016437</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1072996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9F5"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF139">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="43571" checksum="C18C74D3DF18C226" modified="2019-11-13" version="1">MRKYILLGFLGAAVSASIVVAAIVGAKHAHQRSTSSNSDNPSVAARKLQSDVDSYATFPQRLSTNHWNRVQYSAVVIFGASDCDDAHPRSPIFADTVRGAPYWHGRFTNGPVFGEYMAASTLTGNNISLLNYAYGDATVNNGLTKVNAPDTRAQMEMYIGDVREKKAQRGDERTGRVLHCVWIGINDIIQIWTDVIKNGTFQNGNIHGPQTGFEYATGRVHLEARELLDQVKLLHSHPLVNTLHSDFLLMLIPPLEILPNLHYQSKTLAKNNPQLVNTYLEYIGNLTRLYNHELSEGIKALSQEANRAGPDKAAISGNNLAYFDIPKYWYFVRSHPEVYNFVNVMDACWNSTTGANCKAPERWKYYDTLHPSTKMHEYIAQEITVQVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB51</accession>
    <name>A0A5B0MB51_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB51"/>
    <dbReference type="OrthoDB" id="10278009at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022925711" evidence="1">
        <location>
            <begin position="34"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="150" mass="17268" checksum="2479B58B6798D038" modified="2019-11-13" version="1">MFLMNFFQTKHIGLALLPMISLLHSGQVGLADGREVNCKLHFESDDSKADAACWSDENTKFTCPKTSCRSVKYPERHFGQLVYLHCLNEDAAKYDVQVHPRSYHHYPKGDPKWNFVPTGFVAAFDNVSHLWYNCLYDSKKNNQDTYSQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MED2</accession>
    <name>A0A5B0MED2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002628</name>
        <name evidence="3" type="ORF">PGTUg99_004538</name>
        <name evidence="1" type="ORF">PGTUg99_033533</name>
    </gene>
    <organism evidence="1 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000340">
        <property type="protein sequence ID" value="KAA1100451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MED2"/>
    <dbReference type="OrthoDB" id="2511396at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="116" mass="13332" checksum="4BE34516B2294C32" modified="2019-11-13" version="1">MINETSATASHIDLPIFQGLSDLPEEKMKPSKQGMMSENIFFTEESEYFSSDFFHMQYFNDNLGSTFSPYINSISNEKGKKRKYIWDHGEDWHHTFEVDPHGTFSSSTGSAFNQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFG7</accession>
    <name>A0A5B0MFG7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFG7"/>
    <dbReference type="OrthoDB" id="2512063at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="388" mass="43521" checksum="F9C8FDA8862308F2" modified="2019-11-13" version="1">MQLNCVNAQFLLFFHPEFQPTNHQLFPSTLSPPVTQFCERFQTSFSPPEVPLGLEIVQVAQRAALYQPTPWELLTPQEQRDRALARVQLNEEKRIDNLFAFNALKRQHKNCRRELLAELKAKRLAKMSVTSSSSAATTPAAQTAVHSPISGPTTPTCTVPFPQPLDQGAASQLEERVNRLRLITIQKKLQPVLVEKPTEATLVKRDLTTVTTARASVQKTNLAIVPEGTAPATAPVELASDAMDLDPQASSSLASTQKRRESPEVRMLSKEEKIQLLTKEHILLWKRCEAARPSGATEELKALLCQAQESQKTLQKLIPRKELEEFVKGWNPWTIKKELYPSAPKKDGGKKRSSSSKDAKVLYKDPNRWKKVMRGCNTSEAAYRHMAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFM0</accession>
    <name>A0A5B0MFM0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFM0"/>
    <dbReference type="OrthoDB" id="2499804at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="131" mass="15138" checksum="5668A6BAFD77B3ED" modified="2019-11-13" version="1">MVRARLQPWSTDGVDGGPSSITILLKWLATNRNYERWRGRGGESKRELSAEILTEMRRNGIHHRDYHGILLQMTLLERSHEQAWEWEGTTGRAARAIGVDGRMVSVRNHLLRMCPYWDELGPILRPAGEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGX3</accession>
    <name>A0A5B0MGX3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MHYT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_050089</name>
        <name evidence="5" type="ORF">PGTUg99_003490</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000348">
        <property type="protein sequence ID" value="KAA1098624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGX3"/>
    <dbReference type="OrthoDB" id="264015at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005330">
        <property type="entry name" value="MHYT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35152">
        <property type="entry name" value="DOMAIN SIGNALLING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G11310)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35152:SF1">
        <property type="entry name" value="DOMAIN SIGNALLING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G11310)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03707">
        <property type="entry name" value="MHYT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50924">
        <property type="entry name" value="MHYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="MHYT" evidence="3">
        <location>
            <begin position="30"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="825"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="902"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="932"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1076487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1098624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="994" mass="109222" checksum="E102EBD45CA1B7DE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHC5</accession>
    <name>A0A5B0MHC5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014846</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHC5"/>
    <dbReference type="OrthoDB" id="5392716at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="2">
        <location>
            <begin position="262"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="489" mass="55931" checksum="9A312BA8B2EF251A" modified="2019-11-13" version="1">MNNINHHSTTIPETSNHEPQTSSNDIDDLASDPDSVYTTDHSDYSGSDDEHFSSPGDADSEYSEVENTILDLSPRDQRIKNLVEPLFQKGHKGPEIIRILSQDHGIKISPRTLSRKRNEWSLRRCDLSPAPIPAPLSPEVRGSILSSQSKGMNLKEMQVRLYKETGVEVSVRTVQRYMKKLGVKLLQNDLLDGKVTIDRVLEAITDARENLLQNNAGYRRMRMILMRHYNIRVPRQIVYYALKQIDPDGMALRLRQACKRRIYRTLGPNHIWACDGHDKLKPFGITIYGFIDAWSRKILGMFVHVTNNDPRHIACYFLHLASKAGGLPLKLTSDYGTETIDMAKLQMRLSHAYAGITTKQAVDRMHFTKSTHNQKIKSLWSQMMKQHNRSIKDDIMNEIDSGRYDQEDGVQKLTTLPTGVSPEFSYSNPEYFTTTDQLLSVPSDDLDLLSEQQYPDKEILFQHTPPEFNEVALQVMDQLEMVALHPDIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML20</accession>
    <name>A0A5B0ML20_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML20"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="80" mass="8840" checksum="A32905ABE10E870C" modified="2019-11-13" version="1">MLGIMIDPDNETSSHNPRGGSLIKPATKDVTKNERNEKRRTATPSIANRISHPTGPKLLARINQNHNEPLPEPETITSHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNE8</accession>
    <name>A0A5B0MNE8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I and III subunit RPAC2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RPC19_3</name>
        <name evidence="8" type="synonym">RPC19_1</name>
        <name evidence="8" type="ORF">PGT21_024564</name>
        <name evidence="9" type="ORF">PGT21_026506</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNE8"/>
    <dbReference type="OrthoDB" id="510325at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055029">
        <property type="term" value="C:nuclear DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07029">
        <property type="entry name" value="RNAP_I_III_AC19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerases I and III subunit RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033898">
        <property type="entry name" value="RNAP_AC19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF28">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="7">
        <location>
            <begin position="35"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1071979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1078002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="154" mass="17650" checksum="CFC2C9022AA1B0AC" modified="2019-11-13" version="1">MDLEPTHQSTHDPTAEAILADPEKVTILPGDKYDAMTFSLKDEDHTLGNSLRYIIMKNPEVEFCGYSLPHPSEPKLHLRIQMYDNKSAAGALAKGLDDLEQLVLVLAERYRAELDRGKFERFEEPTVDERLEDIKRRKYPHLLKDPQGDITMNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNP6</accession>
    <name>A0A5B0MNP6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNP6"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46546">
        <property type="entry name" value="SHEWANELLA-LIKE PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46546:SF4">
        <property type="entry name" value="SHEWANELLA-LIKE PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
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            <begin position="62"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="409" mass="45797" checksum="ED90E7A16F434EF2" modified="2019-11-13" version="1">MLGLLYRQRRLLITVISLVIIFKYIYRDTSGSGSSKAKPKPKKNHHTQHQSSPSNLKKFSSRIVAVGDLHGDLDHAVRVLRMAGVVDLRNQWIGGPSILVQTGDIVDRGKDTILLYKWMDALRTEAQAAGGAVVSLLGNHEYMNALGDWRYVTKEDIETFGSAESRRKVMSTQGWIGKTWEANYSVTARIPYALGFQELPRRSSQTSTTRRFTESFSEEQDHFETDPFLDAGTVFVHGGITPEYASLGISEINRIGHSLLHRALAGTIPYNHLPPHTPPEEAKLYAEHGPLWERSYALEDDERRICRQIEIATQRLHVRRMVMGHTPQFKGISSRCGGKILLIDTGISSAYGGPLTALEINYSLSPLAHNSSFSAWIESESVFAIQELADKKKLAGFHRIVNLTESLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQV8</accession>
    <name>A0A5B0MQV8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034863</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQV8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022732719" evidence="2">
        <location>
            <begin position="25"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1078416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="248" mass="28542" checksum="4C4B872D04462D9A" modified="2019-11-13" version="1">MKHSCGNQLIASLVFFCILKASHSIDSIVTNKLARNGEVTIDIGTFEPDSGMLSPQDSLNKGEQDKLKKYAGSVPQFRNQQGMFPDLGHPHSTLDDFIPIQESLSVIQDQYAKMILEKKNERIQDKIEFLEDKKQFNLKKDKKRVTHRIFKNKNIKKKTEFGIIIEKLQSIHSLILGEMKKRKGKESQQNFDFMSVDNIQDSQPELLQTIKPASPHELFSQTVALDTIPIHKEIDEVMKRRGWEDQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQZ1</accession>
    <name>A0A5B0MQZ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQZ1"/>
    <dbReference type="OrthoDB" id="10511323at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="64" mass="7241" checksum="5E38674198410EBC" modified="2019-11-13" version="1">MVHQSIHRLALGNLRWRASILSSWVGSPVWKVRRALGDFSYFSAPQHVLLRFVVRGNLIDGLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MT79</accession>
    <name>A0A5B0MT79_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MT79"/>
    <dbReference type="OrthoDB" id="10650723at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="567" mass="63010" checksum="820DFB0052C670FC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTZ5</accession>
    <name>A0A5B0MTZ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016778</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTZ5"/>
    <dbReference type="OrthoDB" id="10444643at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="121" mass="13419" checksum="5F2D08DFDBF69D88" modified="2019-11-13" version="1">MTLSAALIQPPATDLYHEIVPRYNMVSKREMRIVLRLMMSWIRKDNSLPLDIPGCEQLLLEKRGPSSFIRWLAGGTWTLSGIKLATGTGGGRSRSSTAQILLRFSNAQLQHLGPRDGAIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWF7</accession>
    <name>A0A5B0MWF7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005427</name>
        <name evidence="2" type="ORF">PGTUg99_016845</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000244">
        <property type="protein sequence ID" value="KAA1120428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWF7"/>
    <dbReference type="OrthoDB" id="10496330at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="50" mass="5168" checksum="76C49720020FF322" modified="2019-11-13" version="1">MATSTSNGPEKAQNWKTGAMAEESYALRASPPQVAIQVAKSSSSATAAHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0M3</accession>
    <name>A0A5B0N0M3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exonuclease V</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011493</name>
        <name evidence="4" type="ORF">PGTUg99_027986</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EXO5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000408">
        <property type="protein sequence ID" value="KAA1088081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0M3"/>
    <dbReference type="OrthoDB" id="354769at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045145">
        <property type="term" value="F:single-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019190">
        <property type="entry name" value="EXOV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14464">
        <property type="entry name" value="EXONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14464:SF4">
        <property type="entry name" value="EXONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09810">
        <property type="entry name" value="Exo5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="503"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="538"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1082717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1088081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="701" mass="77761" checksum="6A8583BE6FE9275F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N337</accession>
    <name>A0A5B0N337_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003366</name>
        <name evidence="3" type="ORF">PGTUg99_013359</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000209">
        <property type="protein sequence ID" value="KAA1123357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N337"/>
    <dbReference type="OrthoDB" id="2519237at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="813" mass="92713" checksum="07308E7BEEBF1178" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4P7</accession>
    <name>A0A5B0N4P7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019235</name>
        <name evidence="3" type="ORF">PGTUg99_031582</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4P7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="157" mass="17074" checksum="ECC45EC3F9B44651" modified="2019-11-13" version="1">MSPSTRSGRTYSEASSIASSRRSSTTGIAREKQPKSRKPARQHDSDHPPSEEVDPAKESRPDILKGCIADGFQKIHNKAVTTVRTGETTDKTYLNPTIREGQEETNHISSDTDVKPIETPSAVHRRPANNNPTTGAQLPSSTSLQDQSIPTVGRDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5F6</accession>
    <name>A0A5B0N5F6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TAF2_2</name>
        <name evidence="4" type="synonym">TAF2</name>
        <name evidence="3" type="ORF">PGT21_026691</name>
        <name evidence="4" type="ORF">PGTUg99_001969</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000089">
        <property type="protein sequence ID" value="KAA1132218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5F6"/>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="2">
        <location>
            <begin position="10"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1132218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="279" mass="32864" checksum="C29AC5748EC31C58" modified="2019-11-13" version="1">MIRQTSWAEKCNLKPVDRFEGEMPIRIKGSDGIPYEHVPDIKEPFKRYGLPSNHKNKKIERPSRPYASSNQAPDPTETFTYSPWECSQEERDRWKIDDRTEEEDQFITQSKVERFRLDVEVEWIFEFSIEMKEFMWLEELQRDHDVVAQVEAVRALKTIPSKVVSSHLCRVAPVLEYIFQMRNEAVLALDSCATCRCGVLGLFHLLKLFQSRYCYPPRVEPDSPWHIRPIPKPNQFHSARSEQQLSAISSFLISWSILGQHMREKSTTRDQRPRKMLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N689</accession>
    <name>A0A5B0N689_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_002154</name>
        <name evidence="5" type="ORF">PGTUg99_018654</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000209">
        <property type="protein sequence ID" value="KAA1123390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N689"/>
    <dbReference type="OrthoDB" id="2503199at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="686"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="3">
        <location>
            <begin position="80"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="642"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1083638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1123390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="712" mass="77221" checksum="B54FFBD1F25A6982" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6H4</accession>
    <name>A0A5B0N6H4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022618</name>
        <name evidence="3" type="ORF">PGTUg99_022825</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6H4"/>
    <dbReference type="OrthoDB" id="2504933at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052087">
        <property type="entry name" value="RRP12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="184" mass="21204" checksum="09BA10D807DA32E3" modified="2019-11-13" version="1">MKEHGGKVDWKALVNSMEDDEDQEPMDDGEREASIEEYFKMISAGLAGTSPHMVSATIAALSRVLFEFHDELNRTKIDELISTTEIFLNSPNREIAKTAIGFMKVAVVSLKQEVVSKQLEKIVPGLLNWAAEHKNYFKANIQNLLEFFLRLFGFDTFEKFVSSRDICICSYQCNYWYWFIEPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6R7</accession>
    <name>A0A5B0N6R7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6R7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="104" mass="11775" checksum="B7AE811476B8AE74" modified="2019-11-13" version="1">MHRDNPPAITFLIESRQELHLLLTSYRTAPPSPVQPLILVDLAENSSILGNPPPPPWEDPDRLTCIGRDITRDLAKFWAKATPKFIARQPAPSALKFHKKRQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7B1</accession>
    <name>A0A5B0N7B1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031711</name>
        <name evidence="3" type="ORF">PGT21_036867</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7B1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="51" mass="5904" checksum="7C15A7B8F2A662DF" modified="2019-11-13" version="1">MDPTEAKRELADRDATENNCVVRNSRYPNWDPNATGDTRGRQRVQLHRVHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8H8</accession>
    <name>A0A5B0N8H8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8H8"/>
    <dbReference type="OrthoDB" id="2506685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="54077" checksum="BF32262E08DD0114" modified="2019-11-13" version="1">MSLPYTVPDLEDGFAPTTPLGNRPFASGSNSNWNTGFTAPPPPMKKDKGKMPANQYREPQVEEEMSDSEDPTPQRQAKQTEPPVTGYGLHNARGSDGEPTERRYGPRIIKEPGLVYDGTNFTRFLARYERAALAFQASDYEKALQIGRFVHKEELKLELEAMEGYDSFNWTELRKAMKESWGELDNTILYTTADLVKIVEEYSRKGGLKDYKEYKAYLGKFTTILKYLVDNEHLNKKQDASLLFISAFSKESQKNIKRTLVSNGQLPKGPDGSSKPPLWKHVTEAADTEIRVEEEGYFAVSGFSEANKRMQRELDQKKGDGQRREQMISGAPTGKVVEKQVEDIMQEMASLKQQLKSVLPVYNQQSNPKSEFSRGNAKPSTPIYEPMMCFYCHREGHTTYRCSELFKDEEQGLVKRNGKDWYLPDGQHIPWNPSRPIRTVVATASADPKMQEHTNATKTEPKQNSGIMKNSRNNEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9E0</accession>
    <name>A0A5B0N9E0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N9E0"/>
    <dbReference type="OrthoDB" id="10542281at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="243" mass="28040" checksum="B742907AD13A9E26" modified="2019-11-13" version="1">MPKDILVTLNLQHNCHDGKCLIKKTKMVQAERQDTEEHRHMNNLIFHQIDSEDFVEAMSEGHLKWKAHCQKTMPRKKKKEMRALWAHPAEAHHIFLSAKIRGARTGDDNQSCKPWSGLARFPSEGMVKGWRTDRPCSPISFTQRWKVLLGERNLIDNVGGKEATHRQSNGPANRAPTEPFSQPGRMVWTTVHKRSYNMAVRPLWSTPSSNQWVVLASGLAFLKILNFMKIFFARTFSRKFLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NB11</accession>
    <name>A0A5B0NB11_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001584</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NB11"/>
    <dbReference type="OrthoDB" id="2508019at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="58" mass="6685" checksum="A9709C82A5D65E12" modified="2019-11-13" version="1">MSIDDRAKDQMNTSPDARRITPDWTRIVQLTALRLCQWPMKLEELCHTSRSHTAQGAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBF3</accession>
    <name>A0A5B0NBF3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003758</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBF3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="165" mass="18068" checksum="D90A14DE7E3BA55D" modified="2019-11-13" version="1">MESAPSYTLTHSPIAVAKKVEQGTGVAGQQERPHWISRGKAYESGSSSSTSENLNGESKPEQNQEVGYLGHLNLRPENNQGRESENPDSHVQELTVEDMPPYVQLIVAFATESLNLFEKVVDVTADMSPSKISRKAALISVPMCLIIFCAVHYLLSLKVGESRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDS5</accession>
    <name>A0A5B0NDS5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
            <shortName evidence="3">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CKB1_4</name>
        <name evidence="5" type="ORF">PGT21_029969</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDS5"/>
    <dbReference type="OrthoDB" id="2275560at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000005">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1087367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="383" mass="43369" checksum="7CBD0D26C9A1786B" modified="2019-11-13" version="1">MDHDARQRSTDISEIQNQSGSRQRKDNNNEPGGGNPRPTAGENQHQEDEEEEEEEEEDEEEEEEEEIEQEEQENVGGLAEDPAEPDENLVVDDSAHEQLSPGRVAPREGLNYDTDTSASVTDSLTWISWFCSLAGHEYFAEVAEDFIEDDFNLTGLTTYVPFYKEALEMILDVEPEDDSVKVPEVSLVESSAEILYGMIHQRYILTRQGLSQMNAKYESAHFGYCPRVYCQPSKVVPCGRSDVPGDGEVVLFCPNCMDIYHPPSSRYHCIDGAYFGTSFPHLLFQTYRESTSILAPSPTASLSSPEPPCDLQSKSGFMSDSVTPYNSSRSKIYGPRIYGFRVSERARSGPRMQWLRIRPRTEEQLRLGLEYRERELVEEVEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFY7</accession>
    <name>A0A5B0NFY7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFY7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="221" mass="25027" checksum="60FD47E95776C9E8" modified="2019-11-13" version="1">MKQSMSERTTSQYDGITTITVLEVKTASTLAHCLTAGVVRCTPFSLINTLHQAVMTLSGVNSPSRTPPGFMIEASTIPVSFLPIPSTSTSPRSSSHDTLAFTNITQIQICKYHPVKQRRTSGKKEQPIETKATWPSNIHWIFEEQSIWTDPWLRKKPRHPHRLYISIHFVRNMTFMGTRANGTSHTFVFSLMYLCTLLHSQHPSLSFGVHPIVLRVKFYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGG5</accession>
    <name>A0A5B0NGG5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SigF-like NTF2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036016</name>
        <name evidence="2" type="ORF">PGTUg99_019880</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000409">
        <property type="protein sequence ID" value="KAA1087873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGG5"/>
    <dbReference type="OrthoDB" id="2344312at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057514">
        <property type="entry name" value="NTF2_SigF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35393">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35393:SF1">
        <property type="entry name" value="SNOAL-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24840">
        <property type="entry name" value="NTF2_SigF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SigF-like NTF2-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="174" mass="19885" checksum="A6D273D89FED0A82" modified="2019-11-13" version="1">MDDPVKELANVVRSITEPFAAAEIARNVDKYFTEDAFIMYPMFNQPRTPHGRANLKGIYKLLRVFSVNNKIEFHALMFSEDRLNATVDLTETLEARFIPCLKAKLHFITRVDMRKESDGKYRISRQEDNYPNDLKRSGIPLFLIPTLTATYKIVAGTIISCVGRFLLEKGLLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGN2</accession>
    <name>A0A5B0NGN2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016436</name>
        <name evidence="4" type="ORF">PGTUg99_005738</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000408">
        <property type="protein sequence ID" value="KAA1087943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGN2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137468" evidence="2">
        <location>
            <begin position="22"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1082846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1087943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="77" mass="6935" checksum="0A533148E8EDA06E" modified="2019-11-13" version="1">MNFSITCIVLLSILSISSCAAAPAGGEGASKGGGDGGDKKAKAASVLQTVSKGLGGGGLGLSSGITGAVGAGLKGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHH0</accession>
    <name>A0A5B0NHH0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHH0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023082942" description="Secreted protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="8205" checksum="38229932FB19BA1C" modified="2019-11-13" version="1">MNSIKLAIALIISTVFGLVTASNVIDFYTAEWRSFGCDPSEVALCIVTRYKKPNDRSSGPDFYIREPSYHPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKN9</accession>
    <name>A0A5B0NKN9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_017680</name>
        <name evidence="9" type="ORF">PGTUg99_014060</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKN9"/>
    <dbReference type="OrthoDB" id="28755at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF91">
        <property type="entry name" value="GENERAL ALPHA-GLUCOSIDE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="539"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="606"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="479"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="515"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1070605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1089797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="639" mass="71456" checksum="B7644348996755C1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQE8</accession>
    <name>A0A5B0NQE8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQE8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023013487" evidence="1">
        <location>
            <begin position="23"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="90" mass="9937" checksum="9847267B774C9E8F" modified="2019-11-13" version="1">MKKVLQIPLVFLPHFHLGLVISGRPSLGPCPQKQANICEYSDHVKEVNFQPAHADSGSVTNIPSIKTEAFLLDERNAVNHQRLGSDCGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWL0</accession>
    <name>A0A5B0NWL0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015154</name>
        <name evidence="3" type="ORF">PGTUg99_026622</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWL0"/>
    <dbReference type="OrthoDB" id="2499499at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366307" description="Hydrophobin" evidence="1">
        <location>
            <begin position="21"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12093" checksum="DC8EEC0F80ADB27B" modified="2019-11-13" version="1">MKFLAPVTALMVLIFGLAHGQDIILDKWCADNYGNVGKPWAICGQANWEDNNPNTGILTWSVQPQSNCNLAGAIPFCCQQSIRKRVENNPADKPPQVDGAIMLDECHRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYD9</accession>
    <name>A0A5B0NYD9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014347</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NYD9"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805:SF1">
        <property type="entry name" value="GP-PDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="2">
        <location>
            <begin position="42"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="331" mass="37032" checksum="4CB377C6038C87D2" modified="2019-11-13" version="1">MSATQHQRPLSDDSLQSSGLPIQTDDQATGTAAVNKPNNGLPLCWGHRGASATFPENTLCSFEAAIKDGAEGIESDVHVTADDVVLMFHDPSLERTTDGQGMIGSLPYVDGIDAFRTLKAPHQKIPTFDETMDLLMLKENQHVVFNIDCKPNNDPERLFRLIKASIERFEDHATLLSPRLVLGLWHPKFLDPSARHLPDLRKVYIGSSPLTARKYFWEACQGFSMKFSCLVGWEGLSFREDCQKAGKSLLVWTVNDRQEMIEACKWGVDAILTDKTADYLALRQQMKDDWSAVNAETSALFPYSSIHYNGLVSWLRGRVESYVLPRLSVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYZ0</accession>
    <name>A0A5B0NYZ0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">O-methyltransferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_050206</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NYZ0"/>
    <dbReference type="OrthoDB" id="2410195at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="209"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1093916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="312" mass="34052" checksum="736AAE36F4EB13BF" modified="2019-11-13" version="1">MPASTAQQLVALIGQAVQEIEADIGTQIPGGTISNINLPIVAREDGLEASGRRREGLKTLQAATHQLLSTLMPSDLYIFDTYACFFQKTAIDTVVGAGIPDLIHSLDPDSSRGGVHLHVLAQKTQMDSRKLSHILRFLALRNIFCELTEHHWANNRHSFPLRTDSSNSVLNALGHMREEIALPALVQLPQLLLDKQADGALSMDPQQSAFQKYYKPGCDFFEFLAKSDGGYRAERFGKAMFEMTQAIGTGEAVYQAFDWQKLGPSGTLIDVGGGVGAAAYALSTYLPGWKVVVQDRAEVVKDGQEASCNQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZN9</accession>
    <name>A0A5B0NZN9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028917</name>
        <name evidence="3" type="ORF">PGTUg99_032533</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZN9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="61" mass="7043" checksum="2BB4F0260250AC57" modified="2019-11-13" version="1">MRKEVPIESSFYTHSLAMGLSISARRRKNYQKEIRKRSQSTHPEGQATAQPSKSDANDIHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1C2</accession>
    <name>A0A5B0P1C2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1C2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="89" mass="9952" checksum="F74E928951547DAD" modified="2019-11-13" version="1">MDLGVIPNKTNVNTLSVIPNKTKNERRHRRTDQARYKEEPEFVVELAPAHPDIRSDSDIRSRFQQNQTSASASASAGGYPRGYLQISAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1T1</accession>
    <name>A0A5B0P1T1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pseudouridine synthase RsuA/RluA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_000684</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000072">
        <property type="protein sequence ID" value="KAA1095545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1T1"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="2">
        <location>
            <begin position="121"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1095545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="467" mass="53279" checksum="36003D3A53688DF5" modified="2019-11-13" version="1">METPVTHIHSSATKYDHKPTWFFENGLRKVRPYLFEFSTYAKERWRGRSVIDVFSTDFRDRSPEYYAHAVASGVIKINGKNIKKDVIIRNGDRITNTLHRHEPPVAGDPVRILHRDDEKGLLVVEKPGSMPVHPTGRYNYNTLLSILRFDYDLPLVHTSNRLDRLTSGVMVCALTVEASRGLSKYFQHRGCSEERVYRSLSWKFPRHAKTIFKKLSYDPESDSSVLHCRPITGRSHQLRVHLQFLGFPILNDTIYCNLKAWGPSHGKGGIFFAPTMQTEATAEDEPAPNESEGVAEGSGYVEKTEDSRVSLNSQQKRPSKSKLGRNQRQGISQVSEKRVKLDPESAEAGNCSGVPINQLASAVILELRKARDVEDDFGRVKDTIHIDKAFKSPLDLQLSVNANPQPNLPFDEKFCADCGIPLLPDPKPEQLYIYLHAFRYQTDSWDFSSELPWWANEQEWKTRRPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P581</accession>
    <name>A0A5B0P581_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CIT1_2</name>
        <name evidence="8" type="synonym">CIT1_1</name>
        <name evidence="8" type="ORF">PGT21_001113</name>
        <name evidence="7" type="ORF">PGT21_002066</name>
        <name evidence="10" type="ORF">PGTUg99_013679</name>
        <name evidence="9" type="ORF">PGTUg99_014570</name>
    </gene>
    <organism evidence="7 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the citrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000045">
        <property type="protein sequence ID" value="KAA1134819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000011">
        <property type="protein sequence ID" value="KAA1137197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P581"/>
    <dbReference type="OMA" id="YTVIFGI"/>
    <dbReference type="OrthoDB" id="8017587at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007128">
        <property type="entry name" value="PRK09569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF15">
        <property type="entry name" value="CITRATE SYNTHASE 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1096002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1112534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1134819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1137197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="468" mass="51676" checksum="228BA19436F7F375" modified="2019-11-13" version="1">MASFLRRTALDCCRLPRLAGRANQARLLSTKSIHDELADMIPERLEMAKNLKTNYGDKKLGDVQVSHVFGGMRGLKAMLWDPSVLDSEEGIRFWGRTIAECQQVLPSAKDGSEMLPESMFWYLLTGKVPSEKQIEAFQADLAERSTKVPKFVEQLIDSFPKTLHPMTQFVMATAALNHNSKFAAAYSSGVKKADYWKSTLEDSLDLAAMVYPIAARIYVTKYKGGAKNLPAINKNKDLSWNFAQQTGFGDSQGFIELVRLYNALHTDHEGGNVSAHTTHLVGSALSDPFLSYSAALAGLAGPLHGLANQEALRFVLEMKEAVGDKPSAEKVKEYIWSVLKSGRVVPGYGHAVLRKPDPRFAALREFGNRRPEVAADPVFQLVDSLYQQAPGVLTEHGKTKNPFPNVDAASGCVLYHYGMTEFEYYTVLFGCSRAMGGLSQLVWDRVHNLPIERPKSLSMEAIAKIVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5N2</accession>
    <name>A0A5B0P5N2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">VPS5_1</name>
        <name evidence="3" type="synonym">VPS5_3</name>
        <name evidence="3" type="ORF">PGT21_015061</name>
        <name evidence="2" type="ORF">PGTUg99_014480</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5N2"/>
    <dbReference type="OrthoDB" id="271164at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="172" mass="19344" checksum="9FAF596309C5ADEF" modified="2019-11-13" version="1">MVQSLLELAKGLQVLSTSDLLKPVRNTIQRLAGLHRQAHQWGLNQATNELEGLITTSEAHARLTFNGRVKWWDKLQYSINHLMKIQSNHESIKRSCSPNNQSIVLMYPLAELDDPTSASSVENLVRDLDLSRDHKSSLLPSKSPSESHHQHELLQEQQPAVGHYAFGPKSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P722</accession>
    <name>A0A5B0P722_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">General transcription and DNA repair factor IIH subunit TFB5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_023306</name>
        <name evidence="11" type="ORF">PGT21_027707</name>
        <name evidence="10" type="ORF">PGTUg99_003790</name>
        <name evidence="12" type="ORF">PGTUg99_031872</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the 7-subunit TFIIH core complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TFB5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000371">
        <property type="protein sequence ID" value="KAA1095809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P722"/>
    <dbReference type="OrthoDB" id="354at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006294">
        <property type="term" value="P:nucleotide-excision repair, preincision complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1220:FF:000001">
        <property type="entry name" value="General transcription factor IIH subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1220">
        <property type="entry name" value="TFB5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035935">
        <property type="entry name" value="TFB5-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009400">
        <property type="entry name" value="TFIIH_TTDA/Tfb5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28580">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28580:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06331">
        <property type="entry name" value="Tfb5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01395">
        <property type="entry name" value="Tbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142897">
        <property type="entry name" value="TFB5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1066154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1072633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1087275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1095809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="78" mass="8760" checksum="6D21750549A0DE76" modified="2019-11-13" version="1">MVKAHKGVLVTCDPAAKQLILKLNDQPDHQISEFGLVTPFIIQELDDTHLMVTQECVNALKKQLEAELEKNTFTLDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P735</accession>
    <name>A0A5B0P735_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_002110</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P735"/>
    <dbReference type="OrthoDB" id="2506114at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1097295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="458" mass="51048" checksum="C255493319A1699F" modified="2019-11-13" version="1">MSSSVNSQSDSNTIEPPHPPPSQSTHTPPTNPSPQNNENSSNEHSNSDKPTTTTTTKPKKKLNRQLASCSGCRKRRTRCDRGRPCSECYKRNSACDYSGASAPPSMPHTSIAEQVEREEYIKKLEARIQALENSVPSASDHSQSSTPANLAQDFSVDDLASQLSIITIGQRVRCPTGLTHPPHPLRTQFESILASQSNIPPITFIQPDQQFSLDLVARYPIPTLEELCSECLPPRSQIDFLSGFFFTTLNLWTHSINPVDWKIQLDRFWSTPDRLHFRPSPSKPDPPHLTKHYLAQFVSILYGIIGHGLARLADIHNLETVAENQSSGDSSASSPPRDYHLWNLNQAEKISLSNRWFRFSLGLLMSPEGNIYIKPTIFGIRAMAILSNVEHAPENIDHGMIFWSLTTSSAMSAGLLREPPVSDDEDIKTLDETELESRRQLAWAILGLDWCVFSLGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PH21</accession>
    <name>A0A5B0PH21_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025346</name>
        <name evidence="2" type="ORF">PGTUg99_013311</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000208">
        <property type="protein sequence ID" value="KAA1123456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PH21"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1123456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="93" mass="10174" checksum="EDFEA25F29B8C3C0" modified="2019-11-13" version="1">MCWLAVAPDRLCSGCGDALTPQAKPGEKKFQPCWGGTDESQCPFHVEITVFGCDRCPSTVWSAKKPCSNQHVLNDVIRAWRVVGSSTTHTHAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PK53</accession>
    <name>A0A5B0PK53_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PK53"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="165" mass="18667" checksum="1C0EB8FFDE1F4DB3" modified="2019-11-13" version="1">MNKCHQDLVRVNKDRNIQKSAWRAALEVSNNEVTTEDIDATNQLEPVIGINDERDEVDNDGSRLDKAEAIPLGTFGEPDGEFDDEEAELSWMEFVDVAVYQINSELEDLTLDLSSPLYCRQEEEINKLEPNNSAWYPFLNRESSPLGSLANNASEDTDPDQSHYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKY9</accession>
    <name>A0A5B0PKY9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKY9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="83" mass="8850" checksum="3FBF06794072859D" modified="2019-11-13" version="1">MNAISHLFKLWHWPFGLKVGGAAGNVTIATCLRGLGSVVVILRGVERSTRDSTLVELPSSSCSGCVVDGGDKRLMSKGFYIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQC8</accession>
    <name>A0A5B0PQC8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQC8"/>
    <dbReference type="OrthoDB" id="2509967at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="338" mass="37704" checksum="D5A98018E8C3BA19" modified="2019-11-13" version="1">MGPVIPPEGPVCLVPIGCLDPSPLIPLFVLPAFLPHQKNRSTSSTSIILIRLSPKKANLSTLVQAFKAGSLAFPHPLRSPAEKIDSKKKEKQQQHERHTMYFSLPSSLVIYSLLLAGSTVVCPPNNYETLGSTLKIKEIVSSRYAKGEEAALNQPLKEQPNLLALARKQDPEMTRRQKMLTFGRSYSIGGKVFGKIWEGIKNGWRPIGSFFKRMVTRFDEDIDKKDLKTLQDAKRANKLPDTLFDPLLPHHSPDDLNHQHSSATYDPAHHPATQEQPYFHPAQTTYPPPSFDSAHHYPAPYADHPPQPAAQFPYNDFYHHGSTSSGARHTETSSGSHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQG2</accession>
    <name>A0A5B0PQG2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Replication factor A protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">RFA2_7</name>
        <name evidence="2" type="ORF">PGT21_036758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQG2"/>
    <dbReference type="OrthoDB" id="25571at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014892">
        <property type="entry name" value="RPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08784">
        <property type="entry name" value="RPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A C-terminal" evidence="1">
        <location>
            <begin position="84"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="171" mass="18893" checksum="9EB1582884E16961" modified="2019-11-13" version="1">MSVMSRSVEWFVILVRNATNVLLQVGDGTGGIEARKWIDSSDGESEGFEAETGIQFRESDRSSSIPIIRVTLAYERGATGAGDEMNIDQSTNHTTHGKIIQAKIPSRTPKQNKIMEYFASVQPPAEGINIHDIARQCNLDVKEVIDETAELIHEGELFTTVDDEHLMTTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRQ2</accession>
    <name>A0A5B0PRQ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRQ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8413" checksum="DE270638F7946425" modified="2019-11-13" version="1">MLLSMERNSGPLCVVGPSGYPEEEQLDAARSAGDPIAIPSDASSPVFFQTSHPIPTQWDIGIHNSLKRVSKWLLFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PS42</accession>
    <name>A0A5B0PS42_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PGT21_017769</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MYSc_Myo17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chitin_synth"/>
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    </dbReference>
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        <property type="entry name" value="DEK_C"/>
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        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
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        <property type="entry name" value="MYSc_Myo17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF45">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10 15" id="KW-0518">Myosin</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="966"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1005"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1265"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1662"/>
            <end position="1682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1688"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1721"/>
            <end position="1740"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="19">
        <location>
            <begin position="1"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="18">
        <location>
            <begin position="1029"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="domain" description="DEK-C" evidence="20">
        <location>
            <begin position="1943"/>
            <end position="1998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="638"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="15">
        <location>
            <begin position="693"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="896"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="656"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="134"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1103430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1998" mass="222719" checksum="5FEB2B292387ABDE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVF0</accession>
    <name>A0A5B0PVF0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_032019</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVF0"/>
    <dbReference type="OrthoDB" id="2506775at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1104802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41597" checksum="013F76BE1E632600" modified="2019-11-13" version="1">MPKITEENEDGNEGDNEDSDSNSEAGSDHTEEDDAESDDDDEDDTDESQEANKGAKDGQKESSSKANRNNSNDLNDLVTALDFVVKKITGSCAWRQEFERRAAGKGLKNLIAGYGIRWNIIYESRTRAYEAREVIDAILHDEYDKYKQQRSKTSRKENPKKLGHFKEIQFTKKEWAMINELNEELEPFNRLTKLMEGDGPTGAFILPNYYKIISELKNKEDACNRGHPMHPMYVKMIKKLETYENEALECHTLIMATLLHPKLRLKAFTHCWPEKADFARKLLEKEFAKRVEDLKKQKEDNIQLVEKEAPPAEQSDIFEMFNAPARTDESKELEVYINNMDRLPLPASNDPKSILIWWKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZJ2</accession>
    <name>A0A5B0PZJ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033748</name>
        <name evidence="3" type="ORF">PGTUg99_032468</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZJ2"/>
    <dbReference type="OrthoDB" id="2501252at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="238" mass="26803" checksum="193D0497254F6117" modified="2019-11-13" version="1">MSQAIAPPTQTESRLQFGMPTALELHRLELLRAASHHLPSIPPSNPWACLAFRQISHRLIGWDQLYPERTGQEHIFQNYFEESRLGPELLYAIYPRTVLHWNSVPTLSDTIIFSNLDQHPNHPAGKIEKVGDCQSNETSSSSDGSDSSIINSDDAHEDEGQEPLINVSVARPSLILQPMIQLPVMNYSFQLESHSQPTAITHQPTSSSSSNPRYRKSHLIESSFDGMIDFKRRKIQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q027</accession>
    <name>A0A5B0Q027_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035933</name>
        <name evidence="3" type="ORF">PGTUg99_022918</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q027"/>
    <dbReference type="OrthoDB" id="10572009at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033847797" evidence="1">
        <location>
            <begin position="23"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="326" mass="36939" checksum="4533894F888BDBFA" modified="2019-11-13" version="1">MSPLFLIIVAFCAQCHLQYSLSSSVDIGSGYPRHHSPAEEISYSTLSSSTLSQSQNTNSLLLTRRSITRSRLGTIEEGEEEGVDERLTGDVENRTAVPPVEKPIESIDSCKGTCSVDLKQLPASNTAPVHIGPPEVEQRFEEQTSSHASEISDPPEAAREIRCKYPNLYRAWRECIPSDELNKAMSNFESMKKTGFFQIPLWIDEGLDVSRMLQIINFRINGIKLRHIDKPIDQVTLSEQSKIVKLFSGLETEGNFGKTLVRKKEYAQLLEYDKGIQDDFEKFTEISGKKFTQRVRIKAKSNFKSFSDDFGAALIIFERFRKMRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2M3</accession>
    <name>A0A5B0Q2M3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inhibitor I9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_014096</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2M3"/>
    <dbReference type="OrthoDB" id="2506678at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052471">
        <property type="entry name" value="PBI_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288:SF2">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inhibitor I9" evidence="3">
        <location>
            <begin position="134"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1107445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="235" mass="26108" checksum="91421D1CA4D6383A" modified="2019-11-13" version="1">MRKNCLNNSFLILILTTIITIVLILLSASHQHPEPIMSVNNSQGEEELHLSNSQSTDAHPHLTSAFTDSQPKTESSTTSSSSPRPEPLHQHTNDPDPSNPHPLASDPNEGPKQAAPGTHSSESPEPDQEPKIDYIITLKQDTTKGAHSSLPSSVDLQVQKKLLMDNHHSTLSLFPCTEQYELYKQQLIDQGAQIKHEYFSIILKGYAVSLKPSKLQSFQQDPLVKDVEMDQKVHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2Y0</accession>
    <name>A0A5B0Q2Y0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015434</name>
        <name evidence="3" type="ORF">PGTUg99_032042</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2Y0"/>
    <dbReference type="OrthoDB" id="2514083at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="97" mass="10728" checksum="306858DD47EDCD02" modified="2019-11-13" version="1">MSDTTATPAGHEQTTDSARSNRPADGTDTSTAKEWFKAVLKIQHTSIAQAQEDCRQALEDRRADQQIFLAAHQASTDCIRQLEDLLLGMNIKNKVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4K5</accession>
    <name>A0A5B0Q4K5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002694</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4K5"/>
    <dbReference type="OrthoDB" id="10274669at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="56" mass="5938" checksum="7C57BAEB2E72F820" modified="2019-11-13" version="1">MNCLEADQKSSLESALRGLLHSGRLSATGANGLHSPNLMKPRSISIFLLTNTDKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q505</accession>
    <name>A0A5B0Q505_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008731</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q505"/>
    <dbReference type="OrthoDB" id="2495489at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="414" mass="48751" checksum="CA3D76D0170B055B" modified="2019-11-13" version="1">MISQSSVFWQRLEIFAAKENLRPLMDAYRDLCHYFENGAPLNKLFEYYQLISRITLEFKEFKENETRRMLSAHIKRLSQLGKHTEGQSRKLDGRIAKDKVENVLRDKSNLFLNYAEELCEDTQAGNIGAFQPNHRATNYQLYQIASLLCGIFSPLHEMKPHEVDYMSLINAQFNLRINKTNLPAIIKHKMNSFSTVLQHQATLYAMELSMEENDPDKQMWDIWGKGFIEAFKIRKEKFNPDLKPLPLKDNMLIWHTVKSLIDREFGGMDEANAEILLKHLDRVHRAVQSRYVFIEIYETIKKINNLDEREKFMQSFGHQMELLNPNNGKPHKLMKQWEFNDLEKVYDSMHRHLCDESLGLWEKKVFILISNLSVDLQMMLNDIFQKAAEEFIIPKLLVTNMETEAKDSVLDKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6A8</accession>
    <name>A0A5B0Q6A8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein-lysine N-methyltransferase EFM4</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Elongation factor methyltransferase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">EFM4</name>
        <name evidence="8" type="ORF">PGT21_022431</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that mono- and dimethylates elongation factor 1-alpha at 'Lys-316'. May play a role in intracellular transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6A8"/>
    <dbReference type="OMA" id="PTPSFQF"/>
    <dbReference type="OrthoDB" id="10069295at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000624">
        <property type="entry name" value="Protein-lysine N-methyltransferase EFM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03188">
        <property type="entry name" value="Methyltr_EFM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026635">
        <property type="entry name" value="Efm4/METTL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843:SF5">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE METTL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Methyltransferase" evidence="7">
        <location>
            <begin position="94"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1108708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="267" mass="29119" checksum="73D1799AD5803109" modified="2019-11-13" version="1">MTDDGGTIEVESLVPSRLGTQAYWDDNYGRELSNFCEAGDEGEVWFGEASSDEILDWIARYLPSPMTPTKLSFSTGTDEDGQLPGPLGNGRTDSQILDVGCGNGQLLFLLAQGGYSVDCLTGVDYSASSIELTSQIARAKGIQGLRLEVRDVLRDTIEPPHRTRISGSKGWDLITDKGTFDAICLSEETIDGKKLETIYPEKIFDLLVKPGGIFLITSCNWTEEELIKKFVSPNIGFKFHSKIPRACFSFGGSKGSTITTIAFQTCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6M6</accession>
    <name>A0A5B0Q6M6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6M6"/>
    <dbReference type="OrthoDB" id="10269906at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="133" mass="15481" checksum="5D38D6F6AEB325F4" modified="2019-11-13" version="1">MLDKLPQEFHSFKTNISMNFETEVFEEVLKKLEDFAAQNQLNEIKELSIPTQATMYTQSSDPKIACPHCKQGFQACSHCFKSGHSEANCFHKHPDKRQPKTVNSSSKQHLSHLKRYTAEDEDYLKQKYPDHHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAY2</accession>
    <name>A0A5B0QAY2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAY2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="13483" checksum="37D1F64B8A6D82CA" modified="2019-11-13" version="1">MASVGPVEPSQQGKQLNRPRIETSKGKKGIYSLLCQPTNATGLASRGSGHGVTSHHHPSALLRDTSFILSALILTHAQSFHSSRQVGSILANPLLRYTRPASFRLTIDSVARVCVEKKPDSGLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBJ5</accession>
    <name>A0A5B0QBJ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016541</name>
        <name evidence="3" type="ORF">PGTUg99_019591</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000140">
        <property type="protein sequence ID" value="KAA1128648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBJ5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="102" mass="9037" checksum="051D69133ADBC640" modified="2019-11-13" version="1">MSNRLASGNNGTQVDADGSGSGGPGMGGRGSGSGASGSLGDAGAGGLSGPGESTGGGSSGHAGGAGFGSGSSGAMSGAGSGDDGCACKSDSSTHLRIRSLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFL1</accession>
    <name>A0A5B0QFL1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_016419</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFL1"/>
    <dbReference type="OrthoDB" id="2497874at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051373">
        <property type="entry name" value="Lin-28_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109">
        <property type="entry name" value="PROTEIN LIN-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109:SF1">
        <property type="entry name" value="PROTEIN LIN-28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="4">
        <location>
            <begin position="287"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="180"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="399"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="727"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="894"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="727"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="742"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1111932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="946" mass="101683" checksum="9521A79494EC67AD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFQ0</accession>
    <name>A0A5B0QFQ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFQ0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="142" mass="15630" checksum="C6490B2B9AFC0E0A" modified="2019-11-13" version="1">MNPGRRPAQHLGGNRPTNPPVVAPGSFQAWYPIVTPPGYQRGYQPVALPYAPPPFNSVGTQPNRRPDLYRPDNQQRATTQTQRAPTHTLANSADVGDAPVPSNFYQPPEGYEQSEHPSPPQEPLARMIEIGVFDEGLEILII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHV3</accession>
    <name>A0A5B0QHV3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clr5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007317</name>
        <name evidence="1" type="ORF">PGT21_023042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHV3"/>
    <dbReference type="OrthoDB" id="2496728at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1087119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="18756" checksum="228654C064888E44" modified="2019-11-13" version="1">MRITLQNFGHEFQSIVTELINAGHNDNEIRQFLQENHSIIVSQRTLTRRKEDWGLILHASQQMADTEEHIKKYFDQGLTYSQIHHALTTSHNYTHSKRTLQRKITAMQLSRRLDDLDTARVTIEAVVSCVMHLHLTPEGRNVGYRRMRQLLQTKFGITLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJM1</accession>
    <name>A0A5B0QJM1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJM1"/>
    <dbReference type="OrthoDB" id="10639988at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="247" mass="27824" checksum="8FC826B96FD245BC" modified="2019-11-13" version="1">MDPAIITKRCMWGSERGATSTFEVVESIRKCICSDPAGRLRWQEYILSQAKECIKREGRGRARRGPFQSSNSVTADFFSLDSKALREEATCEKEMPFLYSLIKSRLVGTQDSVEDNESNNFDSNDLSGSESSNSSDEETTEAIHAKKGALDRRGTRKNLRTHVAEEELIRKIAISSSSLMNPIICIDNIDFEQSVHSKSAEKQSRMFHGTWGYVHNPDPKILETLNPLDFSVQSYRDAIANNQVSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMG8</accession>
    <name>A0A5B0QMG8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-isopropylmalate dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LEU1_3</name>
        <name evidence="1" type="ORF">PGT21_005708</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMG8"/>
    <dbReference type="OrthoDB" id="2279155at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="118" mass="13085" checksum="FC5838AF3AD54070" modified="2019-11-13" version="1">MFKNGMLPLILPQKEVDQLAQIASEGNDVEIDLVNQRVNATNQQTGSKVSIEFSIDPFRKKCLVEGLDDIRLTELLDDKIKSYEAVRSQKWPWLDGIGFGGKVPIPVGGSATGKNLDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNM9</accession>
    <name>A0A5B0QNM9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNM9"/>
    <dbReference type="OrthoDB" id="2506484at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="280" mass="31341" checksum="315607B62008DFCA" modified="2019-11-13" version="1">MATTKKFSHPMLSRTLADIFEQLKHNIAPGSTYGQLFYSTSVAFTDPDTQQEKNILFKLAGYGSAITALIENHVYLVLGRLIPRKTKSTPVLHYGSTLIRSLTLAMSKQEVSKTKGGANNEILHVVLKHTNYDPLVKTQVQFKAMYHIGNRKNLANTFGLFKLGREILISGNIVGYNEEQHMWIINASNFCLCYLWTPVKYDPKPEPTDIQTEALAIRNPVSDLSGGPEQLTSQLYPTQDKKEDNYYATVTSTSGKKRTQKAILADEKRAKKALPSTRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPA9</accession>
    <name>A0A5B0QPA9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ISA1_2</name>
        <name evidence="6" type="ORF">PGT21_029590</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPA9"/>
    <dbReference type="OrthoDB" id="333486at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.300.12:FF:000001">
        <property type="entry name" value="Iron-binding protein IscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050322">
        <property type="entry name" value="Fe-S_cluster_asmbl/transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072:SF41">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="5">
        <location>
            <begin position="194"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="99"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00054873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1115018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="302" mass="32995" checksum="7D735A72BE371010" modified="2019-11-13" version="1">MSSSIPYSLIQKLTQPSSSSSSLLSRTNRLFHHQSPSTSIHPGQTGILLRRSISHRFSHSQPSSSHQEHPSISLNQTRNLEISNSNQVIHQSISSVNQNNLKLPRSSSSTISQPSEPSHEDILINSLPTSYNSSSTTTTASSSSSLEPPSFEPLQTQRETTSSAPNLTQQSPTKAPTSQVKRTTRTSKPAKQVINLTPRAIGHLSTLLDGPNPKLIRIGVKNKGCAGMAYNLDYVDQPGKFDEVVVTKNEEGKEIKVLIDSRALFSIIGSTMDWQESKLGNKFVFDNPNIKEECGCGESFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPG6</accession>
    <name>A0A5B0QPG6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein PAN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Actin cytoskeleton-regulatory complex protein pan1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PGT21_032068</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the PAN1 actin cytoskeleton-regulatory complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013182">
        <property type="entry name" value="DUF1720"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF173">
        <property type="entry name" value="ACTIN CYTOSKELETON-REGULATORY COMPLEX PROTEIN PAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08226">
        <property type="entry name" value="DUF1720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">21-0</strain>
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        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="114" mass="11633" checksum="34DBB8EF65E42DD1" modified="2019-11-13" version="1">MRFTVAIAAALAALSSVSAQNGMQCGVNHALIPLDLLQSNSCSMTGMGGGPNFMQCGGDAGDGFFGLGAIVSLDLLQQNKCSMSGEPGPFDSFAAGGGLFRKEANNQKKDDKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWU0</accession>
    <name>A0A5B0QWU0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWU0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="287" mass="31655" checksum="44AC89014A9D715E" modified="2019-11-13" version="1">MTNPLQSISDALKKSSSQHSTASGSEPSRDSHTSQGSKTPSDPIQSTPGPKAPPPSLAPPAPSKTNPAQKTADGKSQTQTQPKHLSGKNPMAPPFQPSKTASALGYPQIIEIPNPRLAKEKAKEMLLAKAIKAQEQGDDEKADRFFAMHDTLLKETTAIVAQDDREIQVVTKDPVVPQKRPLAAEGTTEAKGIKFIWERSNSHDDGGFTPYFHKNLTELKGPIPLTIFNRKWQEEALSYHSKNRPKTDETSAEKGLRYHGLAVPDEWLQTFADWTLNHQCFYETIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXC6</accession>
    <name>A0A5B0QXC6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXC6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="118" mass="12918" checksum="18E9AF6FE4A0BFAE" modified="2019-11-13" version="1">MPQDSDLAAQDNKVGGEQNKAKQTDDQEMNSPKEKEGSDVDDNLLDEGAEEDDPDQENEAADAQEEESDNEVPGQDNTVPMMDHVDNSEVLDLPENLELEDHGSEAGENSLTRLNYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1N8</accession>
    <name>A0A5B0R1N8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011609</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1N8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1118965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="87" mass="10248" checksum="CC1588A53C318FAA" modified="2019-11-13" version="1">MLTGTEWELGVVQGTARFNRPRPYLRRRRRCRRIEYRPVIEGQRRQQVVRSYGLIAPVSPSSDRAPRLFLLPSRYPVPPYPIPLPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1R3</accession>
    <name>A0A5B0R1R3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1R3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022684651" evidence="2">
        <location>
            <begin position="32"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="470"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="563" mass="61056" checksum="2FCC730BF4F1686B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2F3</accession>
    <name>A0A5B0R2F3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-zipper-like transcriptional regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LZTR1_28</name>
        <name evidence="5" type="ORF">PGT21_032253</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2F3"/>
    <dbReference type="OrthoDB" id="2496273at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051568">
        <property type="entry name" value="LZTR1/Attractin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376:SF1">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="4">
        <location>
            <begin position="474"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="369"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1119707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="744" mass="84854" checksum="6E4995A09EC548EB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4Q4</accession>
    <name>A0A5B0R4Q4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine/threonine-protein kinase 17A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">STK17A_1</name>
        <name evidence="7" type="synonym">STK17A_2</name>
        <name evidence="7" type="ORF">PGT21_007134</name>
        <name evidence="8" type="ORF">PGTUg99_002490</name>
        <name evidence="9" type="ORF">PGTUg99_011837</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000243">
        <property type="protein sequence ID" value="KAA1120478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000210">
        <property type="protein sequence ID" value="KAA1123226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4Q4"/>
    <dbReference type="OMA" id="IDWFESK"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001194">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1D, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="33"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="342"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1099423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1120478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1123226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="397" mass="44137" checksum="9DADB5ABA3999C7D" modified="2019-11-13" version="1">MPDSALASLHPKRRVLRALGLRDPNLHARKSEFKWGAHLGSGTYGEVTQCTWLTKNPPQEVAIKVVAKSSVPNLQQHISLMNRTLKLHHPHIVQMYDWFQSKEHFYMVLELATGGELFDHTITRGNFNDREACEMIHQVLDALSFIHSQGIVHRDLKPENLFVRGSAPYVPPYDLVVADFGVAVYVDRPDGSTASLTGICGSPGYTAPEVYRRLPYGKPVDIWATGVIAFILLSGRFPFAHLSGQAFLDDSDAEIKFPKRFAISPQAQNFILGCLQTEPSKRWTAEEALEHPWFDQLEEPIEEDVGERQTAASVADTDHTRCEQLPLVRADEPDDKQVESLATTAASSAERVGSALPPPSSHLSTPRSDDDPEPQLVLVHRSHTVNEDPPLVVDIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RB33</accession>
    <name>A0A5B0RB33_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007454</name>
        <name evidence="2" type="ORF">PGTUg99_005421</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000213">
        <property type="protein sequence ID" value="KAA1122946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RB33"/>
    <dbReference type="OrthoDB" id="2513820at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1122946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="64" mass="7169" checksum="FD10F4A4D1BB51C8" modified="2019-11-13" version="1">MTTSDSKYTSEQFFHPVALRDCKLRPTKVQSRLIGHNQVSIEVASDSVAAWVNVEHPPGRFSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RB95</accession>
    <name>A0A5B0RB95_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGT21_008527</name>
        <name evidence="21" type="ORF">PGTUg99_005609</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
            <strain evidence="21 23">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 17">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">In the C-terminal section; belongs to the NAGSA dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">In the N-terminal section; belongs to the acetylglutamate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000213">
        <property type="protein sequence ID" value="KAA1122947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RB95"/>
    <dbReference type="OrthoDB" id="438291at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04252">
        <property type="entry name" value="AAK_NAGK-fArgBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23936">
        <property type="entry name" value="AGPR_C_ARG5_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24149">
        <property type="entry name" value="AGPR_N_ARG5_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000029">
        <property type="entry name" value="Bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000011">
        <property type="entry name" value="N-acetyl-gamma-glutamyl-phosphate reductase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058924">
        <property type="entry name" value="AGPR_dimerisation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041734">
        <property type="entry name" value="NAGK-fArgBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011241">
        <property type="entry name" value="NAGK/NAGSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006855">
        <property type="entry name" value="Vertebrate-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04768">
        <property type="entry name" value="NAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22698">
        <property type="entry name" value="Semialdhyde_dhC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036440">
        <property type="entry name" value="ARG5-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51731">
        <property type="entry name" value="GNAT_NAGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 17" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16 17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12 17" id="KW-0521">NADP</keyword>
    <keyword evidence="9 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="19">
        <location>
            <begin position="358"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="520"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="524"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR036440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1064581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1122947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="891" mass="98854" checksum="04B939BB2210EBA8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RF07</accession>
    <name>A0A5B0RF07_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Large ribosomal subunit protein mL59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024546</name>
        <name evidence="4" type="ORF">PGTUg99_016645</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1123992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RF07"/>
    <dbReference type="OrthoDB" id="2504282at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037507">
        <property type="entry name" value="Ribosomal_mL59"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040922">
        <property type="entry name" value="Ribosomal_mL59_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18126">
        <property type="entry name" value="Mitoc_mL59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL59" evidence="2">
        <location>
            <begin position="41"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1123992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="299" mass="33827" checksum="ED887DAC04C8ED60" modified="2019-11-13" version="1">MLSTTAPTIRIANSSRLLKQSNQFISPHSILLRTKKAVQQRLKKIRTTRPNNSPSLLKILSSTSSKDHQGLGGESPFLPTKFRSDHSNPTIDPAKVRWRAPIIKAQARAQICKQAFHWRILRYVFNLTADSLGPTGGDQQKKMSRSTKNLLSQQPNFYDGSLSLEEFKQIPTDPVPKVIELLGGEDRLTVKDKRLLGLFQQPKDFIKPVLNSVSSSAAAASPQHDPLTQPRYSSPTLVPHSFGPYIGRRKPFKGKIRQRNREAKVAEVTQKMVKMDDRIQSYRKEWKIVKEKSKPALPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SLK5</accession>
    <name>A0A5B0SLK5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020536</name>
        <name evidence="3" type="ORF">PGTUg99_006640</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000002">
        <property type="protein sequence ID" value="KAA1138425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SLK5"/>
    <dbReference type="OrthoDB" id="424465at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF6">
        <property type="entry name" value="2-OXOGLUTARATE AND IRON-DEPENDENT OXYGENASE JMJD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="1">
        <location>
            <begin position="166"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1138425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="424" mass="49377" checksum="72681B7B0A637465" modified="2019-11-13" version="1">MDVLAEDPSYSTFLQSYLLPNRPVLIRSKMASEWPASILWTTPVRQELSRAEDRREDLTGLVRSYGHLEVPVVKQRIQSSSSDFLDDLESFPDWGKRITSQENDCMPFSQVAAHWEKKLRDPTRLNHREGQAVLNDEEVIYVKDWHLIRICQQTNEPENLDEERHSERRNATPFYQVPEIFLDDWMNDYYSAETDDDFRFVYIGEKGTTTGLHTDVYNSYSWSANIVGKKKWRLFRPESDESITIEQSPGEIIFVPSGWKHEVLNLSPLVISINHNWCNSVNLPSVYDALAKDIEDVKESIADVKALLRKKWDNQLAESSGDPTSSGWEHEWIEVVQELTKQHSGWNWATFWGMVKFITMRDFTEASTSSLFDQPVPQHSPPLQFVKTQINLCVMKFKMRDEFNFDAKLRLIIQDIESCLSMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LIG9</accession>
    <name>A0A5B0LIG9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UTP23 sensor motif region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_013657</name>
        <name evidence="9" type="ORF">PGTUg99_014213</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in rRNA-processing and ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UTP23/FCF1 family. UTP23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000515">
        <property type="protein sequence ID" value="KAA1064159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LIG9"/>
    <dbReference type="OMA" id="CCMQALY"/>
    <dbReference type="OrthoDB" id="25675at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09865">
        <property type="entry name" value="PIN_ScUtp23p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057776">
        <property type="entry name" value="UTP23_sensor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24779">
        <property type="entry name" value="UTP23_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP23 sensor motif region" evidence="8">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="183"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="275"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1064159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1092771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="290" mass="32463" checksum="D7F582CBD822A95B" modified="2019-11-13" version="1">MKTKRNKSNRKTMRVYNSIFKFREPYQVLFDADFAVTVTTQKMEVQSRLAAVLGGTVKPMITQCSIHHLVNLASDGSQVAQEAVDLARNLCERRKCKHGKIKASSIECIKGILGDTDNRLRYIVCTQDPHFRTYLREKIIGVPIIYINRSGTLILEEEGPATELRRKQLQEDKLHVPPEELEQLKSTDTGPDSGLPAQLIQGPSTTNPQQTQSSDTLSNGQTHSAHKLENKLLKKKKRRSGGPNPLSVKKKKSSASNRRRIPQEKSASKNTPNQKTSSKSSRKTPPGKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJJ7</accession>
    <name>A0A5B0LJJ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_050067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJJ7"/>
    <dbReference type="OrthoDB" id="2506088at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="157" mass="17850" checksum="AB5517BC9FA047CD" modified="2019-11-13" version="1">MLLHLPASIKRFGPASLFATEKFESFNGVVRNAAIQINRHSPGHDIAIIFSNYQIEQLLVSGAHLYDSTVQEYFKPSDKVTDVFSRNPLIQQAMGYNSTALHESQYPRVKDTHVVQANLELVPEDIREMYPNQQVWQVSSLQLNDKETIQKGSFDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMX9</accession>
    <name>A0A5B0LMX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_020039</name>
        <name evidence="3" type="ORF">PGTUg99_018055</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000512">
        <property type="protein sequence ID" value="KAA1065180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMX9"/>
    <dbReference type="OrthoDB" id="10366826at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036136994" evidence="2">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="66" mass="7187" checksum="964A5C16DEF3BFC3" modified="2019-11-13" version="1">MQLLQLSIFTLAAVLMASLQAEEVKSEIGAAENKLAQVDDSIHSHEKRGLEKRKKKKGGGAKKKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSB4</accession>
    <name>A0A5B0LSB4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPL8B_2</name>
        <name evidence="6" type="ORF">PGT21_020801</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSB4"/>
    <dbReference type="OrthoDB" id="29563at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="85"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1067887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="185" mass="19897" checksum="BD2C43DC8D5ABF87" modified="2019-11-13" version="1">MAPPHPDIPSLFQRNWTSASAGGYPAATAVSTDTRPLEFGEGPDARFFLPAGEVQQFFKMLEGCRLVHLTISVTLVGTVSTTSLLDLIEAKKASLVVIADDVDPIELVIFIPALCQKMGVPYCIVKNKARLGTIVHKKTAAVVAFTDIQSEDKNELAKLVSAVKKCPLYLATAVANLAQRLCESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LTK6</accession>
    <name>A0A5B0LTK6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyroglutamyl-peptidase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_015014</name>
        <name evidence="7" type="ORF">PGTUg99_026938</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LTK6"/>
    <dbReference type="OrthoDB" id="407146at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.20:FF:000015">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1067705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1093552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="28727" checksum="668784A454D09CAD" modified="2019-11-13" version="1">MPSIPTRRPIKILLTGFGPFRNHEKNASFEIVKRASESGGFDSEDGNSAVELVVHPKPVRVSYRTVDELLSRLYHTHPDLDLVIHTGIAQLPRAEFLLEKIAHREHYKSPDVDGLLPDPSLSDSETRPASLSSSVMLDQLVERVQDQISQPINLKVSENAGRFLCEYIYYSSLLRFENLTNSNPEPPSTSVFKKEEADRARPRPVLFLHVPPFLDQADITLGSHVLLDIIHQVQALHFSSSSDNLYKKFLGSSYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUK6</accession>
    <name>A0A5B0LUK6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_017364</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUK6"/>
    <dbReference type="OrthoDB" id="3980884at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023066863" description="RING-type domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="291"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="154"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1067796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="359" mass="40627" checksum="F6CE3C9105E60479" modified="2019-11-13" version="1">MKLAFVQGLIFVQFLCLIALQVTAIDDIESDEEVQRYLYQVYGLQVHYDSTPERDTLGHDYGQGGRLIEAIGRRFTHLLPNRRSISARRTGRSREAPSSGMDGWVARYSEMPFFEPVLLFGSPSRYDETKEIKNTVLFGYMSKHEYLQLSALKNDGTTPSHQVDQPEQSAVTSTPHKRPKALRTQLRRISSLWRQKSRVKIFQPGPKQHDTVPLTEVLDVLADASVTGQSSSSGRETPPDWIKHLYTEASRVNDKNGKQAKSTTTLSPEHHQQTINRISDVLLGESDEQTCPACLEDFQSPRPGPNQQISVDSLEQVLLIQTCGHYFHRQCLQKWILGEKQNSCPLCRNLVNPPEQPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZ51</accession>
    <name>A0A5B0LZ51_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase sgs1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020474</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZ51"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23098" checksum="7288454941ADC618" modified="2019-11-13" version="1">MRVAEDRMQEKVLIAEERAAELQWVREQAKATRALDKQRWEILLQEGRSPVEDQASGNNGAEASGTDISLGLDSVGTNKRFNAREGQAEELACRQFNRIMAQQRKVEEQARQRDEASQVLLTDLKEKQERRASRQAEAATLRENNEACKAAAKRRRLDTIVNNRMAAGSNRAKSAKEHQAKLLRKARTEETMAEVRSELERGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZ56</accession>
    <name>A0A5B0LZ56_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030529</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZ56"/>
    <dbReference type="OrthoDB" id="2496784at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="309" mass="32938" checksum="69269106F589C2CB" modified="2019-11-13" version="1">MVLAQVSPRTSKYSASMFFAIGLVHLLCLSHPIGSASIKLLDSPDHSIDSTREGHVRAVKSAAHISRRSDTGLSPAVANSGCYNYLKEKDGCAYANPTNNCARKMSSMAVQLFAMGPPPGNTSSHIDQSSLPSEKALLKRNENQTWSAPTPKNTTSFLATNSILSSNSSSNSSVFLGNTSLPLYNNSESSICGNYDGEEQMGVCLWSGPSSGTPDSNGSGWVSSNITGNCHKEVILQRSGSNSPMIVARVLEGCSFSTEELSTGCSQIYITKKVFMALNPSPTELEKGALEDTITWDFNSRGNIFNSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2M3</accession>
    <name>A0A5B0M2M3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2M3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="10690" checksum="CBA26FEF9683B65E" modified="2019-11-13" version="1">MPLGHQLSPCYFHPRSAISTHSTQNHTPDGLITPGHSAGRLTLHSSVITFSRLKHFILAWLERCGHLHPSLLGLNASSSLGASSLPGSNTADIQKSRSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6Z1</accession>
    <name>A0A5B0M6Z1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018893</name>
        <name evidence="4" type="ORF">PGTUg99_015851</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000174">
        <property type="protein sequence ID" value="KAA1125889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6Z1"/>
    <dbReference type="OrthoDB" id="2536450at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862:SF1">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366106" evidence="2">
        <location>
            <begin position="20"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1125889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="302" mass="29296" checksum="DA85084E52B0FA12" modified="2019-11-13" version="1">MFSLSLSATLLVLSMGGNQLVVNGQAATNASGSTTREGGAGSSSSSPSSAMGLASGLSPGCQAAAGGLLTSDFGACSNIMGLVSVIGASGSVIPPLTNWISGVCSASPCSADTLKQANQTVSSGCSSDIQKGSGIAMALQTIVSNYNGAKDLLCTQYTSNSTFCVPSILGNVQTATGQNITISEVTSLLSGSMTPAGQAFANVPSGTYCTDCGHALVTQSAAFLASSNGGGSSGNASSGGAMSSISNTCGASFTDGKLPSTVRVANKNAADNAKNSAAVRLPGSLLIAFSVALSASALALLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7Z3</accession>
    <name>A0A5B0M7Z3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA wybutosine-synthesizing protein 3</fullName>
            <ecNumber evidence="2">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_006583</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TYW3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7Z3"/>
    <dbReference type="OMA" id="TWLYVSH"/>
    <dbReference type="OrthoDB" id="48625at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1960.10:FF:000003">
        <property type="entry name" value="tRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="12">
        <location>
            <begin position="23"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1073257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="307" mass="33639" checksum="C90BA8E139631CF3" modified="2019-11-13" version="1">MDEKTVSEYKNFEVEREQIALNLAELKDKSTKGTIDEPIRPFLDLINAQPDWISTSTCSGRLVSYLPGASAEAGSSDPSDHSQRVRQAVNGKGGGTWLFVSHSTLETNQLANPIQTLFGDSYRIESSPNPDSWTCDDSSSLVVHFVYQPPVFHLLAKSVTVAVPILTTVIGAGFRNSGITVGHRGRVILAIRASTGGLDVPIAIQKCSEKSTLQLLVPVDYLSKLLLQGNQLMHQNQAKLERLFEALSNSFNAPSLKSINQWESAEDRRQRMRLEGLAAREASLVSSQSNPTQTSANDEFPELSLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAI3</accession>
    <name>A0A5B0MAI3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAI3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="136" mass="15128" checksum="BBE3F4787F8CE461" modified="2019-11-13" version="1">MWQAVSGSLQLVCRELSCRHQGFWLPSDSSGALDVLFRISTPNPIPIIVGLFIMAWPLAVVGRAYSKGIDTSGSGFLGFLMCFPCYRYVHSRTTGSRKDPARLFLFITPRMAMLFPGTEMSTYPFIDIATLHDDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB09</accession>
    <name>A0A5B0MB09_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_002007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB09"/>
    <dbReference type="OrthoDB" id="2506366at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="5">
        <location>
            <begin position="709"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="344"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="513"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="518"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="572"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="587"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1073158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1108" mass="125820" checksum="146CB3BBBBFF3F42" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MD97</accession>
    <name>A0A5B0MD97_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011464</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MD97"/>
    <dbReference type="OrthoDB" id="2496461at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="198" mass="21866" checksum="6F32C69E8AA12965" modified="2019-11-13" version="1">MINPNTLIQHGKKVDLFAKRAAQQAAIKKDCALSMKCKLTREDKSKGGTTPPDRDLEPKDWDNINFHMKQIYNKYPIKSNYNPKILVYVDAGNTDKYILLTNHTCQEWGIALAGIAEGVDMDTPPQNLPMKSLQASRYKKSRVVAPAPGQHPAPAPPQMPSIMEIIAVADTLNARRGKGDSEGVPNVNAQDFCWGPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ME46</accession>
    <name>A0A5B0ME46_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="9">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_002163</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ME46"/>
    <dbReference type="OrthoDB" id="437526at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01720">
        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="94"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="31"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1074348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="202" mass="22673" checksum="57018CDCA6387D6E" modified="2019-11-13" version="1">MKTSSISLISAVGPAAVPRFWPLSKGPASLARRIHPEANGRSPRSQIVGPSDTSQRKDRLASKMSQYAHVPKSELDEEKIRALEEHEIATGPLSVLQSSVKNHTQILIALRNNRKLLARVKAFDRHSNMVSPQRSFDMFLSFPSLCLLTSPVFSRVKVLVNVKEMWTELPKGKNKKPVNKDRFISKMFLRGDSVILVLRHNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFA0</accession>
    <name>A0A5B0MFA0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFA0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="246" mass="27070" checksum="C682BE22ECEB8616" modified="2019-11-13" version="1">MSDLTPSEHNSSAMDTGSNSSITSSAPVHRPQIADMLRSVELCVAGLQEHTEGAVQPITGLLHKIEESLEKIQEQLAHGAQPSQSSGIAERNVQGSGTEPQNAASGVSILLNPPEMTVDRITTYDQYVPVFDVKRVPADLVKYVEALKNMNFEDKVLMSSSTIERNELYQDSMLQLKQLESEMIKSALQRPVLPISITHREKFLFDLSVLITKTLNMRGFNSNCSRCDLRGIGEGNRGSNPHCLYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MG75</accession>
    <name>A0A5B0MG75_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000153">
        <property type="protein sequence ID" value="KAA1075711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MG75"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="8491" checksum="8E110487EAC73462" modified="2019-11-13" version="1">MHRLQSQDIREDWVVLSSSISNLQYTESIAVSDCQWRAEPIAVTSKNLNENLSLGAKGSNSTNLKTKNYKHQSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MK01</accession>
    <name>A0A5B0MK01_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015554</name>
        <name evidence="2" type="ORF">PGTUg99_005196</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000451">
        <property type="protein sequence ID" value="KAA1077225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MK01"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137247" evidence="1">
        <location>
            <begin position="27"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="119" mass="13244" checksum="F6CAA0648CD2E4B5" modified="2019-11-13" version="1">MKLHPTLQHVFGFALVMLISFQLIAGTPPLCFKCNESMEHYQKYMTCTLSVTGCPPTHKCGNQLPVPGWICNKCGAVQRASRTNPVCCRDHETSSCVKCDFAGHDKELEQAEWRGSVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMF0</accession>
    <name>A0A5B0MMF0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Stress-responsive transcription factor hsf1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">HSF1_4</name>
        <name evidence="8" type="ORF">PGT21_019797</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMF0"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="7">
        <location>
            <begin position="26"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1077782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="10960" checksum="739E3BF6576A655D" modified="2019-11-13" version="1">MAITKRIHPGALTVDKGSSHRNTERPIPAFVTKLFTMVNDPNTDHLIKWSEPNGDPFFVVSSERFGRELLPKFFKHSNFGSPLLLTNALILTGHSSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMZ5</accession>
    <name>A0A5B0MMZ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMZ5"/>
    <dbReference type="OrthoDB" id="10280018at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="537" mass="59250" checksum="65115EE4AB18657D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MN46</accession>
    <name>A0A5B0MN46_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-ketoglutarate-dependent dioxygenase AlkB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MN46"/>
    <dbReference type="OrthoDB" id="28127at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032870">
        <property type="entry name" value="ALKBH7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21052:SF0">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21052">
        <property type="entry name" value="SPERMATOGENESIS ASSOCIATED 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-ketoglutarate-dependent dioxygenase AlkB-like" evidence="2">
        <location>
            <begin position="154"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="300" mass="33330" checksum="11120E3CB67A6538" modified="2019-11-13" version="1">MAQVMKTLRACSRASMPARPSCSVTIGIIAPSCAASQGPSSSNASSIGIPARRNLSSQSYDLRDATCEWFEIPDGHSEYHKDLYEPTDFVLHPRFLTGQEQDLILDHSLTRLDKMERPRARRRGGAQRVGGTEPRREASRFRAEEEYSFEAGHFDQVISGYREMQLGQFVSPSDTTPTHQSLASILARLQALLPEAPRPPLIHLLHLSSQGRIDPHVDNLEASGSTIVGLSLGSTRVMRLGHQAAPIHTHLKVLLPPGSVYIQRDSVRYNLQHSIPIHDSFKNQPIIGAQRLSIMLRNQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNZ8</accession>
    <name>A0A5B0MNZ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030487</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNZ8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="90" mass="9819" checksum="656B9E851DDADAFB" modified="2019-11-13" version="1">MAPTRIPTEGPSTFGGLVKSVMNRSIVIFMPTDTKCYIYIQWHEGCSSPDGKPDDALADLCLKPGAQALRSSSSAGKWLNRGSPKVFRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQR8</accession>
    <name>A0A5B0MQR8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQR8"/>
    <dbReference type="OrthoDB" id="10290574at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="14993" checksum="D8A56A75233ACB42" modified="2019-11-13" version="1">MEETLRNEALQAIDFLERHKRANTDKLYREALTQEPKKDRYVDTNSKVKSLKEMVARDLGFQGTVKHPRVWHLQTTDVGAPMKNHGTPPAPRRDTQVQVQLPRANHSAMLAFILLSYALLFFLLFSDWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWH9</accession>
    <name>A0A5B0MWH9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWH9"/>
    <dbReference type="OrthoDB" id="2505348at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="364" mass="40613" checksum="023FFCB523209C2C" modified="2019-11-13" version="1">MAQHTSTQSPTTNHPASPIDITIVMQTNNIEDEAPTTPPNIPDTYSNPHGPQSIRRESSRQRTPSHRPGFIPTSNDSRRSFIAPVDKTRKSKPAVPCTTLIDCEDLESESTRSNRINLGTQLKHRDGRVAVTEVRQTASRTGKVVQVDTIQDSDEDNAKAEAIKNKERTLLLDKDGFDHPKLYFYPRGQGPKQDGTVESFSCRWCPNSFKASGNSSYNLKTHRDGGFTKGSTRPRPICPGRAKAIESGANLPPTASELASDKALSHPSSTGTLIAFTSKGHFDNTTFNKLIVIWMVRQSLPWLRIEDFLLRVAFDYALHLTKVFSRVWAALHAHQLYLEQRSQVIQAIKVHKFLIYCSSSFLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MX60</accession>
    <name>A0A5B0MX60_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MX60"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="330" mass="34887" checksum="05D95615B87156B3" modified="2019-11-13" version="1">MRAMEPPAVEMPPYSAFERVFTSASSAQDAQYSQSTSRATIPIPEGFVAPTILPLGFEASNVDPFRPSLGIDSESSSQPQASYSCPLPTHQNGNSGSVFTSASSAQDATNFQSTSRATIPIPEGFVAPNLLPLGFQASNLDPFRPSLGIDSESSSQPLASYSYPLPTHQNGNSGSVPRPTHQNGNSGSAPLPVHSHTDPRLSLNYQTGRNLSPFSPHTFPEINQTKPLHWGFDQFELPVAPVAGPSSLQPYSAPPLGANGNFNFSVPAVIPNGWHPDQATTENFLGYDSPAIPVAGPSSRPYSAPPPGASSRSVKKKNEKSFQKGGCKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3L4</accession>
    <name>A0A5B0N3L4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3L4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="416" mass="47706" checksum="6569467A4AE1CDB8" modified="2019-11-13" version="1">MTPRNRLALAHRFPKRSQVLLGSPEQTLRPSTVTTIIAVIKASKFPKKLNSYHPAPKTNLSERSQLSSFNSEDPQDPIGLLLVSPSVPVNLQENSELIQRYLIILRLRKEEAERNYQPTPWEKLSEEEQLLLATYHAHIHQEEDLEQQIQFRSFLREIREKNSQLRRKKYLERMSTFETGVIRQERASPTPSVDTDNGSSDLPTNLTNQTSKVPSQLQQDLAAAKELQARVASLQMTTLARKEPATLIPETTEKNRSDPPPNTEKDMLMDVDEIESECSEATPKRPITPEIRVLSKEDQIKLRVKEHVSLWRQCQVATREGPTEKLRALLTTAQESQKALQKLIDNEEIESYVKGWNPWDEKKVHFPSPPKKNVMKFKRNDSGKRQSSTSTNFSDPAKWKKVTDLLQMASGLYQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NB26</accession>
    <name>A0A5B0NB26_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022685</name>
        <name evidence="3" type="ORF">PGTUg99_004278</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NB26"/>
    <dbReference type="OrthoDB" id="10366602at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="507" mass="55406" checksum="CA9BACE28756D145" modified="2019-11-13" version="1">MESSGKVTRSKSGGRKEAEAPPAIDPTHVAQPRVDPTNDPVVIDETPDSEGGEDVSLDLIVKNPTLSATVIEDVISKTGPPPEAVKVDKRAMVWDRLNAAQTAGDTFLSKILLAAYNSLEPNPVSKPVITRSTSAFAALSPPDRDVSLKGSPQAVDPNLAETQIEDNLVYAVGTVTSHQDIGFTPYFDENIKKLRAPLPLTIFDREWQKKALNAHLLLKPAKSSEDKAYRGLAFNDEWTQSHSDWTNNHRSFFITLRDVYNKKIFAGKLLKHKENCDSIADTYGFMTAFRYDMQIRMNAFTHRVPSKDGAALPDIAVKQGIVIEQCYSTVRSFGESNWKDNLYAPGQSHAAYDPDTGTKRPELMKASSFPSARGPSNQSGSGGYHPDNHQGRRRDTRRFGRGNGPDQWGNFNYGFHTQGYDGSNYGHNQQYGSQPPSFNNQYRHQFNGNFGHGAASGSGSFGHGAASGSGQGGVDSRKRFRPSHGQGDNEFNAGNKKGSGTDALTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDG7</accession>
    <name>A0A5B0NDG7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_008603</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDG7"/>
    <dbReference type="OrthoDB" id="2504362at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="3">
        <location>
            <begin position="248"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1086692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="339" mass="37531" checksum="5D72E53951069F70" modified="2019-11-13" version="1">MSHCTAAETMTATNSRTRRPMSTSIIPSLSLDSRPPLSSKRASTPYPTHPPKRPSSTSLAALSSSQSRVALNLLNRISTQNSLRSSSESSPPPQFIVRDFAFKSSDPRHLGQRLTTTPNHHHLNGTDEASRLNEFNHQLADQLKQTPTKSMFWNICASPTGPGSTADSEFDRDQEEAEQSMEIDKLIHKNRQNSSSSALSSSSSTPPLTDPTVPLQQQQQQQLMIGENGGEQMLTRLTTKWENLYVSMYDFVAEGESEMNLAKGEIVCVLEIICDGWVVARKTKLLIDDDGKLFSPDRQPVSLDDLNLVLLRLPLPDDPHHPDLSLTGLCPENYLLKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDJ2</accession>
    <name>A0A5B0NDJ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDJ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="64" mass="6931" checksum="F375B0EE4EEB26D4" modified="2019-11-13" version="1">MAAIYFYVMLQAQAAADVTLKFGRNKPPSPSDAGFLRDLPIDRLPAATPAPIPKARDTTSKTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGW6</accession>
    <name>A0A5B0NGW6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGW6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="8042" checksum="BD9BDD7923FE48E3" modified="2019-11-13" version="1">MGWYSKYRLVSGGTFHSAAVAELEDLATWMATCGGDALRADEEDSDSDSDSHSSLESASDQNSEKDDANSDSDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHR0</accession>
    <name>A0A5B0NHR0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHR0"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF64">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01760)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="204"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="398" mass="44404" checksum="54694CE0E7E09C1B" modified="2019-11-13" version="1">MNRGLIRTGIRILRRHAHSYIPDPTHLRRRTAPRLWRAWSALPVARLADLEGCSLAPDGVPWLLVGQLLVGLPVALWTWKCLMLVLFQRRIIYMPYFPPGSRTQELDPADPLLRELTVRKLSFASVPGSPRLDGLLLTPSSLGSKPPDGLIYYLQGNAGNTLARLPVFRELLLPPSTANSPKLGLFALSPRGYWTSGGPFPFPFTALSVPGHRHSEAGIIRDYLRVLRELGERTGLEDVPVWLHGHSLGGAAAAQLLARMCSDKDTVGTGRAAGLILENPLPSIPQMVQVLYPSKWLPYHYLGRFARDRWLSASALQAIPPHLPLLFIQSEHDELVPPSLTRELYNLTRSKRLLDSPSSDPLVHYSVIPHALHDSAFSKPRYRFSIHQFITSTMPSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI07</accession>
    <name>A0A5B0NI07_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI07"/>
    <dbReference type="OrthoDB" id="2507256at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="380"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="504" mass="56591" checksum="051EDA319481BF4D" modified="2019-11-13" version="1">MDQSMSMDQNQSDNSLQLSLSNVNLGRSSNPDQANCQANNSSDLRDLPAPPAPKRRRRTQDEMEVFRAEQEQARLCRKQEREMAKLVKNQQKEAQKQAKEAEKLAKEADKRLKQRNGSRSRRSRGSEDADDRGKKFVHSDFENICSYLENTQHYTDLYGDGSKTGVGTTKMTKSQAFDVFAVWMNNTNPDLLLNGRRLQLRIDRYKKKYLDAKRFKENTGAVIESNHGPQTLAEALERICPCFDRLDAILRDKANVVAMLEFDHSQPGLELPPLEEESDGSPEGDLPPGQQSEAGPTTNGVEEPLLGESPNLNSTDEFTDSLPTSTPISQSMSLAPASSNAPNGSAQSANQRSNQPTTLTPSGPNGGGRQSIRGRQTPLSRSSQMASISASSTSTQPQQAPKVSLASAFASSADSRFAIIERQIQVEQERMEREDARAITKEKQDESRVEQEDERWNRQLDWERERYNRDEQRADNKEMRQVALWNEKKAVIMDLSYQFMGSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIJ8</accession>
    <name>A0A5B0NIJ8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_005379</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the Golgi-associated retrograde protein (GARP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS51 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIJ8"/>
    <dbReference type="OrthoDB" id="203678at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014812">
        <property type="entry name" value="Vps51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU368010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1089057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="305" mass="32714" checksum="C4257BE85067A022" modified="2019-11-13" version="1">MQRKSSLPTVSVSPSRTPKVLTRTTSSSTTSSPQSSAPSTAHQMNFPSHPPVLGKVGTTGDPVLAKRRARNLLRDYYGLGNPTEAANPSGPEEPGLAHVSGSTNDSARLSHPQERLSIDSPYFDVDAYFNKLVGTSSLNSLLSTANELMSEIRELDGERQSLVYNHHHELVEASETIDKMKSSSQKLDGTLSQLQDSLSVISQLSTSLTSTRAPSNLQTPSDPTATTSETALTNKFEDGLHLSALLSLPAVLKGLLATETRAAADQLWGQWEPALRSFEEAGVQGAKQVGNECREALRNAKSFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIK7</accession>
    <name>A0A5B0NIK7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008612</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIK7"/>
    <dbReference type="OrthoDB" id="419631at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="307"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="409"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="558"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="764" mass="87172" checksum="12FC3C6B2C62FD23" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKX5</accession>
    <name>A0A5B0NKX5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">superoxide dismutase</fullName>
            <ecNumber evidence="4">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_011354</name>
        <name evidence="10" type="ORF">PGTUg99_011606</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000139">
        <property type="protein sequence ID" value="KAA1128750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKX5"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000007">
        <property type="entry name" value="Cell surface Cu-only superoxide dismutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053257">
        <property type="entry name" value="Cu-only_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20910">
        <property type="entry name" value="AGAP001623-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20910:SF1">
        <property type="entry name" value="SUPEROXIDE DISMUTASE COPPER_ZINC BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033848937" description="superoxide dismutase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="8">
        <location>
            <begin position="52"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1089242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1128750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="185" mass="19219" checksum="941609243F85CF92" modified="2019-11-13" version="1">MQLSFISGLVCLSLLKSSEAAPQLSLAQAASHLARPTNARATLMSPTGAPIYGLVEFRATATMDVQVQVTVFGLDHSLPHGEHAYHIHANPVGADGNCEATGGHLNPTGIADSVTCNPMVPKTCKEGDLSGKHGVLAGAQRSIQLSYTDPNLQFSSPESGIIGRGLVIHDSKGARIACGNIIRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLX5</accession>
    <name>A0A5B0NLX5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLX5"/>
    <dbReference type="OrthoDB" id="10601001at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="127" mass="14034" checksum="CA809306DE4E018F" modified="2019-11-13" version="1" fragment="single">MVKIMNRATKKKERAHQQLQTGGSERPINVDNSCDAPPNQGRHFTLPTLALGRLKPSWEYVENHCPSKEICGDVDTSNIVSTKCCQPTQITSAIDQQPEIVNLVQPVTMAEAMASPKEKPMCLEAMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NN05</accession>
    <name>A0A5B0NN05_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NN05"/>
    <dbReference type="OrthoDB" id="2506702at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="49104" checksum="1C84D54A16DD1772" modified="2019-11-13" version="1">MSDSSSPLLSTHMRFPSPTATPPTQFLSPHPNYLSPSPSLPPLSPLPNEFQPRPIPPMSPTLLAAAMPYLPVPESPNIIPATARDDGVSSLISHLGIENQSLSDTILSHAQKIADLEIQQRRYLKLFDDLKALLPLSSQFVRIKTNANDQFLQVRNAIDSVNNSTASRLDRVEGFVNHLITHPPVQPKSFRDPPYLSHIYFSGVLSETKEFCFAMRHALEMNAENFRNEKHKVMWIAGYFRKPDGKLGDNCASYVWWRGLMAKNAHHQNLDPNTVSSKAQFLLEELLLGEAFLVAIESTFSNHKGEEEARKSLRVMKQGNTSIEEFNVSFNSLLYAVDLSEASKCDLYDEAINPKIIQLGILRGGWNGVTDLAKKQAMAVELAADVPGVALIDCLSQKPQPIIQKIQLPPRPDSSKAPPRLSDGVPMDIDAIAADLGFTYDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQT1</accession>
    <name>A0A5B0NQT1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Haloacid dehalogenase-like hydrolase domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019077</name>
        <name evidence="2" type="ORF">PGTUg99_015461</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000177">
        <property type="protein sequence ID" value="KAA1125530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQT1"/>
    <dbReference type="OrthoDB" id="444127at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.720">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051828">
        <property type="entry name" value="HAD-like_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044924">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191:SF2">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1090956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1125530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="33580" checksum="6825088ACA428BB1" modified="2019-11-13" version="1">MSGLCHLSSRIQRNGTHPPAHVFLDLFGTLIYPALPIHIRYNQIAHANGFGDFKLSAKNFKTAFKKANQEFPNYGKNAWVTDNQEKHVRMFPKDWWLKVVNGTFEDSLKTQDRRPSGPKISEIDKISQLVYEEFLTNEDEKIYKNYEDTLDFINYLLTEKIGFSILTNSDPKILNALPPSLRELLFSKDQINSQFQAFTSWDIGFSKPDQRIWDSAISQLNLNKSNDRLIHLGDDFQEDFLGAKSAGIGSIWLDRKDNHQKISKPIDNEIANHQFKAHERIHSLNELIGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NS11</accession>
    <name>A0A5B0NS11_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NS11"/>
    <dbReference type="OrthoDB" id="10460426at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="13053" checksum="1C52F8D6D1C936DA" modified="2019-11-13" version="1">MGNTRKRHKDRASSTTSISSSPAQLKGPSRKKRNNAVVDTDGDSEVEQQDTDVDHTTSASTQNLEKDQDQLDAEELKKAMKVHKNQQSACYASYNPPQLSDQLDKNGRRMIAYPCKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NT99</accession>
    <name>A0A5B0NT99_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022927</name>
        <name evidence="4" type="ORF">PGTUg99_018380</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000178">
        <property type="protein sequence ID" value="KAA1125465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NT99"/>
    <dbReference type="OrthoDB" id="2519216at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366312" evidence="2">
        <location>
            <begin position="21"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1125465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="805" mass="93478" checksum="850327B2DCE1DED0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTB8</accession>
    <name>A0A5B0NTB8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-dependent RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031631</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RdRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF55">
        <property type="entry name" value="RNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RDRP core" evidence="2">
        <location>
            <begin position="479"/>
            <end position="1065"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1282" mass="145269" checksum="C6345D108DBBEEB3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUS4</accession>
    <name>A0A5B0NUS4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="12">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ALA1_1</name>
        <name evidence="12" type="synonym">ALA1</name>
        <name evidence="15" type="synonym">ALA1_2</name>
        <name evidence="14" type="ORF">PGT21_018906</name>
        <name evidence="15" type="ORF">PGTUg99_028402</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="12"/>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUS4"/>
    <dbReference type="OrthoDB" id="2423964at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070143">
        <property type="term" value="P:mitochondrial alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000011">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059090">
        <property type="entry name" value="ALA1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26023">
        <property type="entry name" value="ALA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 12 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 12" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="13">
        <location>
            <begin position="56"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="652"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="656"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="772"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="776"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1092951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1115718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1012" mass="112786" checksum="CEBB572F2658ED5B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NV10</accession>
    <name>A0A5B0NV10_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Capsule-associated protein CAP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CAP10_5</name>
        <name evidence="5" type="ORF">PGT21_004313</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NV10"/>
    <dbReference type="OrthoDB" id="202415at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF35">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="4">
        <location>
            <begin position="328"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="315"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="626"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="315"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="628"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1092476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="661" mass="75494" checksum="002CC6F4E303B64A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NXD3</accession>
    <name>A0A5B0NXD3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016880</name>
        <name evidence="3" type="ORF">PGTUg99_019717</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NXD3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="224" mass="24825" checksum="978E0E1EC67462A1" modified="2019-11-13" version="1">MEPRHGTVTTRDLEPKQLYSALQKVERGPQAAKLLMNTAQQNANQETRSYIVADKTYQHTNAWASWDRAEATYTPIDSELAEVLHQAIKNTLAKAEEKEEGNHKQLANEEEAEKEKRLAPGVRANPAHALAPGSVADELPSPAPVNQADEASSSSSAAHRQDQSRCFPSWRARISNRPTERPSTSSSGIHSQTQSARSTEKKWRRIVKKIGDFSEALSILSFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P006</accession>
    <name>A0A5B0P006_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031456</name>
        <name evidence="2" type="ORF">PGTUg99_010265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000444">
        <property type="protein sequence ID" value="KAA1079055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P006"/>
    <dbReference type="OrthoDB" id="2499220at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="443" mass="48678" checksum="4A51DD697EF2E96F" modified="2019-11-13" version="1">MASSPTQEAHSLSTFHWLFSEEMPDESLPQFFPRVLDFEQSSTQPSHGTPFRPGPRYRRDQSYQEDSSPMYDPDCSPCYYPDSPQWAPGPISQSISSPQWVPGPTSQSISSPQWAPGPSSGSISRPTLQLSQAPELPFECQGRKRHTESPQEADQRPRKYSRPSDSQSDSHVDSEKPDGSAAGTPPTTATHSLHSSESPSSSSLACFDHIMEIQRLRAQLVISEARNARAMSDLAEARLCVDFLMNEVGRVMASTSQAHPAAERVRQIIRSNTELRVPLFTRASPEMTTLADSQQDTLPVVDNADAAWSEDLDPRNPNSYYFSEIPMAGPSTRPGPISLAESSQLAPIGGFQGYGSMTSVGGEGSTSYSEMVREASLDYRSGSSLTEAGRSRDPSGSQLVPDWSHTRVDSYYFSEPLPPFDSAQETSRESYAPNYELPATRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0G5</accession>
    <name>A0A5B0P0G5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014447</name>
        <name evidence="2" type="ORF">PGTUg99_028344</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0G5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="75" mass="8362" checksum="D232FFEB055C1206" modified="2019-11-13" version="1">MAWHMSFFSVMIKLKQYSTSVGSSTVETLNARDLNSSTIIVIGSLSTSIIRLDSSWKILAALLNRQSSNYYSVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0S5</accession>
    <name>A0A5B0P0S5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0S5"/>
    <dbReference type="OrthoDB" id="10596031at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1095135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="285" mass="32569" checksum="E2F64A52E384A7AB" modified="2019-11-13" version="1">MFILEQDGWSMQGTDWEEVLKYYLLYSRYQARIESTTNDDPPVIIKLGSFTSIDIKLIKHFTSSSSTHSQSFLIKPSPFAHPFVIPITILRFQFVNVFALIRHRKPRVRPCRFGTNTPFDARLSQFRKRAEETGSIPVLGNLLFDRAATPPYRLLDPHCWVACKIKACGMGRRSRWPASRVLWNCDLCARVKWDGADPSERWLDEIRTADLKMPTHIACLASALKKFPSSTISGNHTDHYLNGRFRSNEIEPIELPIPNALAHNKVIAINTDLIMMAANQQARIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1I4</accession>
    <name>A0A5B0P1I4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013381</name>
        <name evidence="2" type="ORF">PGTUg99_000369</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1I4"/>
    <dbReference type="OrthoDB" id="2509423at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="23249" checksum="83D4A5CB1204B19D" modified="2019-11-13" version="1">MNRPEKASKPLPYCHHHPYHSSHSTRTPKEVVRRPSNPPSHENNHLTKPSDHPEQFAFNINQPKSKVSQSIILKGIPGSADNSSVFKFLESAGAAVCFVNVIYNPKTSRSRRYAYAHFFGLDRAKEFVEPRFPLMSWKEPDNSSHGDYYRTLKIQIDYSTEPPEVKQNRRNDGDDDVGQMPISILLLRGLNKKTTLQDIHPCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5K1</accession>
    <name>A0A5B0P5K1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SAC3/GANP/THP3 conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_028043</name>
        <name evidence="8" type="ORF">PGTUg99_030230</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SAC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1108100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5K1"/>
    <dbReference type="OrthoDB" id="264795at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000008">
        <property type="entry name" value="Nuclear mRNA export protein SAC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF3">
        <property type="entry name" value="GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="6">
        <location>
            <begin position="69"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="480"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1102"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1096765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1108100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1139" mass="127751" checksum="367C051DE3140BB5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P661</accession>
    <name>A0A5B0P661_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_017546</name>
        <name evidence="4" type="ORF">PGTUg99_034930</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P661"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366339" evidence="2">
        <location>
            <begin position="28"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="529" mass="59932" checksum="82B4792FBBF9083F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P6F2</accession>
    <name>A0A5B0P6F2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000587</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000069">
        <property type="protein sequence ID" value="KAA1095569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P6F2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1095569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="50" mass="5481" checksum="7A6AD90AF848460F" modified="2019-11-13" version="1">MRQHLRFGTVGPAELDETISNPAHPAQLISKKIFEFQSANPSSSPSSFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7T7</accession>
    <name>A0A5B0P7T7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7T7"/>
    <dbReference type="OrthoDB" id="10663069at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="453" mass="50958" checksum="5749A64DC6FE9CAC" modified="2019-11-13" version="1">MDARRPSPNQLRTQLGAKRRGLGPQSTRARGSDPTSTEYRGPPPHQTNRNTSFDGALGRPAESPQPYNRRKPSALQENFHYKPACGSSLPNRTSELRLRKPPCDENAVRPKEPSYYSQNEPAADPRMNSVRPRVQQPPSRDTKARRSSQAQYGVEDSQAGLNPAQGNNFRESGENFGGEDHSRIHSRSDSDSSNEYEYSPSRDSRPGDTASASRIIHPGAINTFNQLTAPAARIPTADRSSLVSLVTDFPIDQMENDTDIEPRMADTGGNKSPAVSEDHRETKISKRSVPFSKSGVRKRQFISPPSSYLAPSSSKTQEEAASINDLLSVVGEKNRTLVEDLDHAVKENQTLRNQIDMNEIEKRRSRESLKKLTTKIVTEKEKLDENFSEMKFQLRDRFALEKRQSEISSQEIGCLRKEIHESLEVFPKNQAFMWTKFSNPAVFMPAFKMRAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAU1</accession>
    <name>A0A5B0PAU1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAU1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="105" mass="12205" checksum="1BCF12B582C9E6AD" modified="2019-11-13" version="1">MIDKDALVYNIDFFLRAELTTQRSKTSHTFDTRMPDRRTDARRIRKPPFSRDLNSMKLVPFHPETRFKPLASSRRLPCGLGKERDAHGKFQPGQMTVNQRLGTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBC4</accession>
    <name>A0A5B0PBC4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein BTN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BTN1_2</name>
        <name evidence="9" type="ORF">PGT21_028192</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Vacuole membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the battenin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBC4"/>
    <dbReference type="OrthoDB" id="5965864at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003492">
        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF0">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1098092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="494" mass="54294" checksum="686EF80BA19379B1" modified="2019-11-13" version="1">MAYKLSPIHQYSRELTSHPAHSTPRSEDSDSDSDSEQPPQAPLVIAPATTTTCTTLEGSSDIELAAILDKSSNFRFCLSLFLFGLLNNVLYVIIISAALDLVPSDVPTGVILVADITPSLLVKIGWPYFVKGQIRYARRILSCSALSFAGMMVIVIFSSVPLRLFGISLASLSSGLGEMTFLQLTTRYQGQPGVSWFASGTGAAGVFGAALWWLLRHLGVQIGLGLSSVLPICLSFTYFFLLPPPSLLPPVTIEARGGYAVVPETDQTGLSTELPALLVKSPELTFAEKLRLAKPLLIPYMLPLFAVYFAEYTINTGIAPTLLYPPPQPDSHPVFSLVFKNLRDYYPFWQLTYQIFVFFSRSSMAIFRFPALPRTLIPLPSLLQVLIFCTLLLEASSGFLSRLVSVEWIYFLVFALISLEGICGGLAYVSAYYWLGMDGHDLDQENEFRMACVGFADTFGILLASLFSSWLEPRLCGIQVSHGRTLCREVGVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCS4</accession>
    <name>A0A5B0PCS4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 53</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">VPS53_4</name>
        <name evidence="2" type="ORF">PGT21_029254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCS4"/>
    <dbReference type="OrthoDB" id="10261632at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039766">
        <property type="entry name" value="Vps53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007234">
        <property type="entry name" value="Vps53_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04100">
        <property type="entry name" value="Vps53_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps53 N-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="253" mass="27528" checksum="412CD4045C587F81" modified="2019-11-13" version="1">MDRGTDRLDSADFDLEDAINELCPHESSSASLPLLQILLRQRLLECTHQTAILRAELQAACDSGDGQSGCIENIQEGIGALLTTLSTIRSSSTEAQSVVESITKEIRTLDLAKLNIESAIAGLKRLGMLVNAFDQLTRLAKGRKYRETASALQAVQSLSVHLDQLASSVPRVASLFKALQETQGLLRRTIMEEFSTAFEDKSVAINRGQLADSCLVIDALGPDARLVKKKSLFLKKLIGLWIAKAAATTIYML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PED5</accession>
    <name>A0A5B0PED5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Complexed with cef1p</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_004562</name>
        <name evidence="6" type="ORF">PGTUg99_010318</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000482">
        <property type="protein sequence ID" value="KAA1070948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PED5"/>
    <dbReference type="OrthoDB" id="30179at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006973">
        <property type="entry name" value="Cwf_Cwc_15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718:SF2">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04889">
        <property type="entry name" value="Cwf_Cwc_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="185"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1070948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1099356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="33631" checksum="036EEF2DEB52666F" modified="2019-11-13" version="1">MSSAHRPTWTPAMGKDARMNTKQYSSRDIAAHTKLKYRQPGQNSKDDLARRDLKLELEMAERESAAKRRRAAGLLPINEENQANEKQPGTKLLEDGTDEESKAKRRKILEEALLADKDDSESDEDSDDDKRGSKRHGKGKATSVPLSSHNGSDTGRTNGKNDDEESDDDDDDDDDSDDDESETAELLRELEKIKRERAEEKERQERESNANAALDKEAEAAVGNPLLNLKAALNQNGLSPTNSVSTSSNSFFVKRRWDDDVIFKNQARDVNEKPKKEFVNDLLRTEFHRRFLKKFIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFY4</accession>
    <name>A0A5B0PFY4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_050005</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFY4"/>
    <dbReference type="OrthoDB" id="2505476at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058353">
        <property type="entry name" value="DUF8040"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26138">
        <property type="entry name" value="DUF8040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="9">
        <location>
            <begin position="97"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="212"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1099494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="434" mass="50016" checksum="B3680840CE84613B" modified="2019-11-13" version="1">MMDIHSSNPPSLATQFTYFHSSHTSLQHLKQPTMDNNLLAMLAAAGASLVQLNNTIISIAAAGAAITYLGKMWNTYEPYYGDGGQARYTRFLLNEVRPELFREITRMERSTFNSLVEELKFNRLLKDGRSVCVEEQVLIFLDIVCHNNAMRQTAVKFWRGLYTVTRYFGLVLDSLVNIYPNYVKFNMESCIQPDDISKNPRYKAFKNALGAIDGVFIPATVPANIQSPWRNRKGFIAQNVLAAVNFNFEFVYVLAGWEGSAHDTRVFNNATSKGLKIPQRKYFLADTGYGLRQGLMTPFRGVRYHLKEQAKAGLKPANKKELYNLRHATLRNIVERIFGCIKRKFPILHSAPEVELKKQVHLIYALCTLWNYIRTHKRLENLFDDPDKEQRVVQTTNTDDEAFNVADEDSMMKHRRDRLANKLFLQYVTYKRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHH3</accession>
    <name>A0A5B0PHH3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHH3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="160" mass="17657" checksum="5752C67D32199834" modified="2019-11-13" version="1">MIGQISHSCWIVPLGIFPLHDIAIHSLSIPGLDQQIRSSLAICFIAPVYFQDSLEDIATALRGRNTGSIFCYYHPLDASGDICKPRFSVLSLIARTKVLLAFGSTLSQVNILLKMRRSQLGLSSKVFTNAWLLDFCILRFKRGWTSSSSQPPTANILTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIB9</accession>
    <name>A0A5B0PIB9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIB9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11650" checksum="4A06D801552CC0C2" modified="2019-11-13" version="1">MFLMDRVRLDGLTTHIAEEGKKQEAGRKNAKQGFREQSILLIYPRLPTFDSQLGSGWPESQNDTHSEHPASSNSMRMFVQGEREKALKCISNAQFELNGKGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIE9</accession>
    <name>A0A5B0PIE9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIE9"/>
    <dbReference type="OrthoDB" id="1060854at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF94">
        <property type="entry name" value="PHO85 CYCLIN-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="563"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="585"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="69897" checksum="BA7534EAC1E2F3C4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJK2</accession>
    <name>A0A5B0PJK2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_003333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJK2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="67" mass="7173" checksum="E3F9E76BE9D357D4" modified="2019-11-13" version="1">MGRVASNSANHGGPDATRMQKKNAMLLSPGPAPGPRVFKEQQHLDHDWLAVHPAAIFRMATASIEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PP86</accession>
    <name>A0A5B0PP86_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_019330</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PP86"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022914978" evidence="1">
        <location>
            <begin position="23"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="136" mass="14783" checksum="37B8252228DBF698" modified="2019-11-13" version="1">MNLQATVVLFTLAVMFTAVVQAAPPPLCPWCKKAYGIPCTSEQMTKFKLTPSGPCNCKFPSMKNCPNIVPKANFVCIDSNCLAIFCINPGYTNRKALRPCYHPVADVICRGICPAAGTSEPGPSKPNNNNHDYEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQH7</accession>
    <name>A0A5B0PQH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004938</name>
        <name evidence="1" type="ORF">PGTUg99_022659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQH7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="61" mass="6884" checksum="808DF271679F291B" modified="2019-11-13" version="1">MAYLRIPLRPEITDTAEDSPAHRELVKYEDRLELAKRHALEIVLPVTGHAGPPMVQAGHWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVJ1</accession>
    <name>A0A5B0PVJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033207</name>
        <name evidence="2" type="ORF">PGTUg99_021407</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1099035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVJ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="186" mass="20453" checksum="69558305183D5D80" modified="2019-11-13" version="1">MTFNTDWIPGATFSGSAFSDFVNATPAPDPLPSNDLDTAASDDSNSIAPDNESNLANDTETDNANDEIAVLTTPNAVIFVANESPPAYQIAALPLGSPENPIEIEDGDVANEPRNDEETYERLNSPRFDPVEIVPFQTTVVDEEDVLRAREHINRWFNNHIRHNSQRVLRGRSRRARTAAGPVEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PWG3</accession>
    <name>A0A5B0PWG3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001062</name>
        <name evidence="2" type="ORF">PGTUg99_005390</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000312">
        <property type="protein sequence ID" value="KAA1105089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PWG3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1092397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="84" mass="9181" checksum="9657FB4C521F188C" modified="2019-11-13" version="1">MSAGIGIIPSVESSTVIGQDRLTVGRSASRRLIGAVSQTKLSHFRRDPKNELPNRSINRPCIDCSDPASRVFKRVQAYRNGIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYB8</accession>
    <name>A0A5B0PYB8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023468</name>
        <name evidence="2" type="ORF">PGTUg99_027968</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1121032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYB8"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1121032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="100" mass="11470" checksum="06EBC579993BAE4A" modified="2019-11-13" version="1">MTIPTEYIVFKVRDSRGRCETGVFPVLDSGYQYNHLDAYLEATIKEYNLRGPGSKKEKDSDGKEIKENNLNWISVQLQLTKRMTLAIFGFIKLLLENGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZ96</accession>
    <name>A0A5B0PZ96_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031495</name>
        <name evidence="3" type="ORF">PGTUg99_025815</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZ96"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137858" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12215" checksum="CA58B547EF3BC4B5" modified="2019-11-13" version="1">MTCMKLMFILSAIPSLLLAQAIETEPAELLPFEVLCEHQNSQEALEHVPCNTPYTCVHGFRHEACPITRARKFSRCRICTVHWNFHFLLDCKNHPHTRVECDEHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZA7</accession>
    <name>A0A5B0PZA7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cytosolic Fe-S cluster assembly factor nbp35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NBP35_1</name>
        <name evidence="8" type="synonym">NBP35</name>
        <name evidence="10" type="ORF">PGT21_031610</name>
        <name evidence="11" type="ORF">PGTUg99_026187</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZA7"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001759">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03038">
        <property type="entry name" value="NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028601">
        <property type="entry name" value="NUBP1/Nbp35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF35">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="114"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1106242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1109291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="390" mass="42149" checksum="CEECE1F590A43EEF" modified="2019-11-13" version="1">MAIENHLTKNNHSAPNPSSSSSIGPSSPLQKKAISASDTTTPTVPENAPEHCPGIESELAGSSTTCNGCENQSICKSFSTNVNSSQQKQDDLNDLKKINERFRNVKHKILVLSGKGGVGKSTFTCGLGWAFSGDGDNTALLDIDITGPSLPMMLGLSPDVHRLHSTSTGWSPLYVSETLSAMSIGFMLPSTDSAVIWRGPKKNGMIKQFLKDVDWCSTEDEEEELGREETAKENGKENREEGIEYMIIDTPPGTTDEHLSIVSYLKQTGITGAIILTTPQEVSIQDVRRIISFCKKTQVKILGLVENMSGFICPNCNGASEIFLPTTGGADRLCRDEGLELLGKIPLDPKIGKGSDLGVDWLNSHPDSLATKAYYDIVDKVKLKIQKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5Y5</accession>
    <name>A0A5B0Q5Y5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SGS1_41</name>
        <name evidence="8" type="ORF">PGT21_014619</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5Y5"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="52"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="243"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1108507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="409" mass="45323" checksum="C62E0EB9136A289D" modified="2019-11-13" version="1">MTLLEMEPDKPKAPKRLEGLVDMDKTALMEHITTRLVELYKDHPKDLQVEAVLSLVRGGHTFIHAGTGFGKTRISEMYFGLFDRKVVVLVLVPLDSLGDDQVREKKSSKGNSALFTFDEFQAILALIVVDEAHMVYLWGLVASKQSKNLIIFAQLEDQAIFRPAYGHIGTRLMATNNVPLLLLSATCRPEAVAAITTSLMLQPSELAMVDGELTRPDIRFIRVKMESTLSSCDDLLRIFAPHTTTAASEAVPMIIYSGTRNRTFQVMKVVNEARDTKKHEYDTKDPFIRRYHAVTGDEDKETTITDFGADKVPVISATMALGLGQNLKRVRCVIHMGRGDPAAIVQMVGRCGRDGNTGLGILFMEPTRKNGKNQLSDFTTGGYQDDDTRMDALAVTTCCLRVALALDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDD8</accession>
    <name>A0A5B0QDD8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_036881</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001010">
        <property type="entry name" value="ABC multidrug transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="556"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="597"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="635"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="669"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="773"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1193"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1222"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1265"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1301"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1327"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1453"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="164"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="850"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1111139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1481" mass="164662" checksum="112362EE240B0612" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QF89</accession>
    <name>A0A5B0QF89_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_013302</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QF89"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000800">
        <property type="entry name" value="ATP-binding cassette sub-family D member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF69">
        <property type="entry name" value="PEROXISOMAL LONG-CHAIN FATTY ACID IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="490"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1111831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="747" mass="84113" checksum="C020D8D9CF2407B5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFW3</accession>
    <name>A0A5B0QFW3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1112097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFW3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="224" mass="24586" checksum="B76327FD431261D7" modified="2019-11-13" version="1">MDQQSTTTNPIPIPISGEEGPGGPNAAHEVAPSQLATDSNHVSGIVQCVQFASDITECPGAGALAEVEEVEFTIDIPPSHADTCPDSCSPSMCHLDWPARYREMQGPHEDDFPSTYPIGPALETLPPHNQTLIKDYFTRLSAAYAELWDEKPSRVPMTEEELSELFGPQGSLDTLENIGDFHRERIESFFQSFERVIKRKNASLGPQLPTLPQLVDSLTNQLVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFX3</accession>
    <name>A0A5B0QFX3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1112107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFX3"/>
    <dbReference type="OrthoDB" id="10553232at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="191" mass="21456" checksum="B7A174A0C04330DE" modified="2019-11-13" version="1">MLRILDQGLETLVLGCSRVPGTHSAPLVDEAFCIINKSSTAHIFNDPSVLLLQLSKKKEKDSQTQRAHQSSPSSKPRHILHARLIQYTHPLLVLYISWIFRTCVGEVHAGIKVFCSAVILQNPSSDFNDLLFIGHHLQKVLNGLLQTSNTYFSRLLEPSIHLSTLACRLRQSAFSRRKQAAKEKTFDSITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHZ7</accession>
    <name>A0A5B0QHZ7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHZ7"/>
    <dbReference type="OrthoDB" id="2504926at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="1">
        <location>
            <begin position="40"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="143" mass="15903" checksum="352B180D257A55BD" modified="2019-11-13" version="1">MLKLMDTSDNSAEGIGQVNSSNQLFLNLVSRQKNSSHASNFQLGASHTLWNIAQNILTAHFGDPTKTNDLGAWHDLHALGIPSDKAVPKKDFDQMINNMEKIHEASIFHCLCVIMGTTQDHVAETPGKIETRKWNQIVEACYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ13</accession>
    <name>A0A5B0QJ13_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ13"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="122" mass="13607" checksum="F38CD3DAFFE9E83C" modified="2019-11-13" version="1">MLQSMPRTLVDLQRYSHSIHRCRPFDAFVDRSVCFDFLSSDRYVSPLLLRNGTLIRSIGLICRRQISQALSRFNVVADRLVSLHYILPWFIGSSQSTPRTSPTSAPACNSTSLGSAKLCTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ83</accession>
    <name>A0A5B0QJ83_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021691</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMA16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ83"/>
    <dbReference type="OrthoDB" id="270284at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021346">
        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="193" mass="22167" checksum="B62F883C3729671A" modified="2019-11-13" version="1">MHSTRRFPNPVSFSISTASRLTWFIEANKQEARTKDPLGQSPNPIHSADLSTVHNLINSFIARNLSLINEETSKQTKHFLNRPKSTSLENLDLLRRSELIEYSTNGFSVIPDLRTVQNLKLLDQSKDEQQCYRSFLARVKLIRLFKDEPTRVMEEQQGATDSFRLDSGLPTPDDDDDDDDDEDEDVQLIEVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJM7</accession>
    <name>A0A5B0QJM7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJM7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022985708" evidence="2">
        <location>
            <begin position="20"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="510" mass="58908" checksum="8B2C8210D7302E41" modified="2019-11-13" version="1">MLLWGIALYTLGGSQLAQASLAELGETRPSRSVAPIQDALDHVDDDLSLRDAKRRRTEVGEYSEQDRFGQDERQTVDQTWLDKQAEIRSMEQASEEIHRSIAHLKVVGGSHEEALNSIPKLEDVHRHLEIRYRRKNKTGILRKPAQYKQPPRRVRFVLGADLSNSGESSSAQASQRSSNLDSGQTVFPSQEWDDLVRLTEPENEFCRHRPDFKLPFFETMFLIGDYMVKSNLLDPSFFKANNFFRPEALTQMIKVDLKSRLYGFGGLFFGFDAHSLLPTIQSLANHKSTKHLHRAIKVLDKENQKELVYDHLGNTMRSLDWLSARAWGEKSVEFGIVSSRFHSPYFIREADELSQMLIRFKGTPPSNDFRNNPIHSGTQHAINLFNQPPKLSDGKRPRLEFLMSYYYLDFLHTYYKEIVDHLQAANLENQKSEKKRKFLGEAIKIFTDPADPWKPKFDKYSRLIFQQKPHDDLEMKEWIEAITSIILELKVSGVIDRAFYIAMTSRILGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJW6</accession>
    <name>A0A5B0QJW6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJW6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023065872" description="Hydrophobin" evidence="1">
        <location>
            <begin position="19"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15022" checksum="C434A72B117DEAD3" modified="2019-11-13" version="1">MLFCKSIILFSVAIGILGSREQGGGHQHERRRAKLGGGLPQVTDDGCATGLYHYCFTQGQAYLPGASDMQTIAQIVSPVDSNGQTVLSCPNQWPRTASCCDQSKFEVLDRVDSPVKSIFITLKSFKNGCPKDKIKQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QL79</accession>
    <name>A0A5B0QL79_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022419</name>
        <name evidence="3" type="ORF">PGTUg99_021047</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000276">
        <property type="protein sequence ID" value="KAA1113694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QL79"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="134" mass="15754" checksum="48BCE24F02C5D998" modified="2019-11-13" version="1">MELMSPSPKKLIKILNKVLNFATNQFDSHLKASERYKTRVFLNTGIPELRGARSKFYPQSFRFDLQCYQEENPTFNFDFEEICSQNTDNFRRGSPSPYSSNTIDKKQKNQIKVDDQSSKNKRGKSIDQRFKNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QLB8</accession>
    <name>A0A5B0QLB8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Bud emergence protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BEM1_2</name>
        <name evidence="7" type="ORF">PGT21_024478</name>
        <name evidence="8" type="ORF">PGTUg99_017507</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QLB8"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051130">
        <property type="term" value="P:positive regulation of cellular component organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06890">
        <property type="entry name" value="PX_Bem1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11879">
        <property type="entry name" value="SH3_Bem1p_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000093">
        <property type="entry name" value="Protein kinase activator Bem1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000050">
        <property type="entry name" value="Related to Protein scd2/ral3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000492">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035550">
        <property type="entry name" value="Bem1/Scd2_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035549">
        <property type="entry name" value="Bem1/Scd2_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF2">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="82"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="202"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="6">
        <location>
            <begin position="428"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="149"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="313"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="605"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="376"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="664"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1085925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1113765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="810" mass="89078" checksum="BDA39B22885EFBFB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMR1</accession>
    <name>A0A5B0QMR1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMR1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="54" mass="6089" checksum="58DAEEEBB7495B1D" modified="2019-11-13" version="1">MAIDTYAVWDPPWSSEESRSRNAAEETAEWAAPDENLVLAGRSLLLLQVESRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP13</accession>
    <name>A0A5B0QP13_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025943</name>
        <name evidence="3" type="ORF">PGTUg99_014313</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000169">
        <property type="protein sequence ID" value="KAA1127053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP13"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474500" description="C2H2-type domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1127053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14794" checksum="145C3D1340BE3655" modified="2019-11-13" version="1">MNFTRLIIVCAVSLQLIMNTLALPPVRCAQIGCGKSRQLSQAEVIGKRFELEGDCGELVWKGAWVNCKNRRIKLYFQCHTCHRFFRQNQGPTRDNPDKTCANHYNKEFVYPEEMDLIGTIGDAGPSKAADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP40</accession>
    <name>A0A5B0QP40_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP40"/>
    <dbReference type="OrthoDB" id="2506921at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="408" mass="46352" checksum="E670723B57B3984B" modified="2019-11-13" version="1">MDPNNTAGEKEHWPQGDLVVRRFLSLISKCHPIDNGDPYLGSRTIQEGFLIEQVNMEKDLLTELRSDLLPQLGRQITDLVPPLDPARLKEDPGLQLRIVSGIQAELEQTLQQIRSALSILCPDPLPSERIRTNDQHLDGLKSFRREGLHYRIIEEILPDVMCLFHQSETLILQMKLSTNKALQYPCIAVTREQIIEYASSALEGIQGGIQWLEGSEFDLVQYDWPNEIRGMDEQLEELLELINGTAHLKESSRIPGPLSDSVVQLSKSLLPILKLSRLFFNKLSKRGMNRKRLSLYTEIRSDQLDRLCKLAGNVNRELDAVAAVLRTADRSDGRFARPACTQIVGALEAHFESALLLLYLYFLPLIPDTDGFPIQNYFKAWFATWHTQFGLVIQNLIATARLFDEDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP86</accession>
    <name>A0A5B0QP86_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="8 11">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_027675</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="8 11"/>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP86"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01488">
        <property type="entry name" value="Uba3_RUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.520:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.20:FF:000003">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.20">
        <property type="entry name" value="Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014929">
        <property type="entry name" value="E2-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030468">
        <property type="entry name" value="Uba3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF6">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08825">
        <property type="entry name" value="E2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01181">
        <property type="entry name" value="E2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="E2 binding" evidence="13">
        <location>
            <begin position="634"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="126"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="165"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="221"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1114939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="724" mass="82866" checksum="8197EDCBA37DC72B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPC8</accession>
    <name>A0A5B0QPC8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_032375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TALE/M-ATYP homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="KN_HD"/>
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    </dbReference>
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            <end position="451"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
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            <end position="115"/>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAA1115146.1"/>
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    </evidence>
    <sequence length="684" mass="73550" checksum="3ACB09EBFD4D0154" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSP3</accession>
    <name>A0A5B0QSP3_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="71"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="209" mass="22680" checksum="8D872026C8FF5E89" modified="2019-11-13" version="1">MGFTIPTVPSIGHVQQTKHLTKDEFIWHIHAYLDSQGLCHFCKKACGSAPGTCTGPLNRSIVPIPPSFVAPPKPLDYKRPNPRNGSQSQAGKPTHPPAGRPSAKVAAVAALAEDEMYAALDEAAVSAIQLMDNELELAKTKKNLARTHEKQPEKVAVNISEDNFFPEMDRAGVAALEDLDKHLATLRHDGSKELLDDEANHVANLPPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTC7</accession>
    <name>A0A5B0QTC7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoinositide 5-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.36</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="11" type="primary">INP52_4</name>
        <name evidence="11" type="ORF">PGT21_013878</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
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        <text evidence="2">Belongs to the synaptojanin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.36" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000003">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Inositol polyphosphate 5-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF257">
        <property type="entry name" value="PHOSPHOINOSITIDE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="10">
        <location>
            <begin position="123"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="488"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="788"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="944"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="513"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="609"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="621"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="675"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="852"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="903"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="947"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1116439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="991" mass="107067" checksum="BFD690A3CFE39689" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVK4</accession>
    <name>A0A5B0QVK4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028174</name>
        <name evidence="4" type="ORF">PGTUg99_031663</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVK4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474687" evidence="2">
        <location>
            <begin position="21"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1116945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="62" mass="6378" checksum="16B4F9BDB6B75513" modified="2019-11-13" version="1">MAVIAKILLSFLILGATMVASPPPPKDPGSAHTDPPGRAEPAPVEPPPARPRPPSHPEPPCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWP3</accession>
    <name>A0A5B0QWP3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWP3"/>
    <dbReference type="OrthoDB" id="2512528at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="170" mass="18125" checksum="40B6B5597F7DEF62" modified="2019-11-13" version="1">MGRPLWSQTKPHPAEPSSDPPPEDWTSTPWPSLITSSSSTKNLPLVTQIPSARWKEVFLIPTLANPSSAPLTSTTSTTSSDPTTSTNDALGLRTLDLNDPTATLPNCSSSPCQPPLPAPISQLMRMMTPLITPLNASSSSSTKAWVFLHPLSSTNSKQFQSISHHSAGPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWU1</accession>
    <name>A0A5B0QWU1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional repressor proteinral negative regulator of transcription subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NOT4_3</name>
        <name evidence="2" type="ORF">PGT21_018788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWU1"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="102"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="131" mass="13863" checksum="321CCB23EB5AAB85" modified="2019-11-13" version="1">MHAPGHHEQHINGGVHGTRERRMDLAGAGFGPYGQTAAADLGSEFNDPAIVNIRMANNPQANNPQAFLALPPGPDQASHPALNEIHAPFSLANAGFNHLLARANPGAGYRPPTQANDNPIQTQFGFQSRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXJ5</accession>
    <name>A0A5B0QXJ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXJ5"/>
    <dbReference type="OMA" id="NEHQNEI"/>
    <dbReference type="OrthoDB" id="2507620at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="356" mass="39793" checksum="6A35A9B89C283A6B" modified="2019-11-13" version="1">MGKEIEEGENSKPSTGKQELDQHQSATSSPPELPPKTAIQSYPSIITTTNHSKQSNKILIRFLFSLSNFIFFGGTLAGLIVWFYQKYIFPKLRIRTEILKSLRLSTLKSYDKLHQSLKSLIQSNPRLYQSTLAKLSGKVDQVLSTSSDAKTGDGLDDAGKASDQIQVSEGSSTTDEKTQVPDIVPSSSSSTSSSSILDEKELQNEPHKPSSDREEFNQPVIDYLEKIAQGLRKRSDNRRPVQIEESDDEQMDESRAGTERSLGGIESLTKSLKSMGAELADEAQLNTRVHQKLSASSHPHRAFGLNHWTASFNATRYPDAPPPKNVDEDPYFIALMEFKNQIRNLKGVLLNRKKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1S8</accession>
    <name>A0A5B0R1S8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1S8"/>
    <dbReference type="OrthoDB" id="2501673at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="400" mass="45357" checksum="9FEC3BC0775E29CF" modified="2019-11-13" version="1">MKSIIKRIKRRASPTTASALLVPPVTTQTQHLPKHTRASSHEQSVRTRETFVTPSNNSSSQLSPDLAVPNNSRIGQSTSQDYKSPESATQVNPSHHIPENMESFISITDSSASGERSYTSRVSANSEERLSSHLPQGEEAQLQSAKNDGLRSPSVNAPSIRTFGDHAHRQTQSNFVHVVPDDSERDSTTTYYEEKHTLKPVTRETIHKHVVEEVQKVKLHERHLTYVQHHVQVSATKLSYLDLFEAFGANRNPICSNVIQPIINPSHELEFQDYRIELPDQPGPQIDRINIEKKLKLDTIIDDLIRNNKKRYERDGHQKVTNSQSIELPDVEHQAMVVHNYHIIQPTVIPSATEAKRTTLEDTYPQQLRQLNKLIDKLQEENRYLKKSLSDALAGRSSVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R6G0</accession>
    <name>A0A5B0R6G0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025065</name>
        <name evidence="2" type="ORF">PGTUg99_023198</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1120968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R6G0"/>
    <dbReference type="OrthoDB" id="10502017at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="108" mass="12115" checksum="69316CE0C142309C" modified="2019-11-13" version="1">MRELGLNALKTVAVHGQIQDKEAQKSEAQKKKKFGIPGARDRKLYGNCCTTHPYQILVLEPPPSHSDFRNSNPESIHNDIIIHQPTPTSSHFSIKLKTVKPKLIPSHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R6Y8</accession>
    <name>A0A5B0R6Y8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033653</name>
        <name evidence="3" type="ORF">PGTUg99_029563</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000239">
        <property type="protein sequence ID" value="KAA1121267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R6Y8"/>
    <dbReference type="OrthoDB" id="10279746at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="101" mass="11346" checksum="62B2DABCA281078C" modified="2019-11-13" version="1">MEIMSKNKEELLNDVSHEFLFLQKGKIIEDLCKQIKQRQKLAAEKEKQTKAPAGSSNQLEALAATSEGDSTYEEDPSSNAKDSQNIGDEDTLEQLHPGWHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RA48</accession>
    <name>A0A5B0RA48_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023950</name>
        <name evidence="3" type="ORF">PGTUg99_002143</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000220">
        <property type="protein sequence ID" value="KAA1122681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RA48"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138081" evidence="1">
        <location>
            <begin position="22"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="98" mass="10920" checksum="5AA84230B7244AF5" modified="2019-11-13" version="1">MTCVKLLCIFAAIPGFLLVQALNLQPDHAANPVPCQHENRETETSHVQCTWTCPQGNNHQCPGILVRLVDRCEDCNVGGLDPRYLHPCIRKPHKCSCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RDF6</accession>
    <name>A0A5B0RDF6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_005747</name>
        <name evidence="8" type="ORF">PGTUg99_026314</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000206">
        <property type="protein sequence ID" value="KAA1123851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RDF6"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474699" description="RING-type domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="172"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1111593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1123851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="279" mass="32456" checksum="D543C8B5940F4813" modified="2019-11-13" version="1">MISLPVLFTLIVRFFTLLSQPLEPLDTSSLKNVRFSEREPPKPQKLKVVCYMPSESAVADGMVSNDQKTIRKSDVVPCEQNYYLNQFVGHPHYEFLKSLKEGLDSWEGMIDDCCERTKKISVSHQRKIIKKLEEPAGKQQISKKQQYKDLDTEAHKSPEDLHIRLLEATSDCPICIDRFQEPKRNNKGQWEWEKLYSCPACNWCFHIECLDRWLSKEKALTCPTCRTLIDNGRRHMAILERVQTPRLHHLEIPLSEELLSDSDELYLPSPEAESQVNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RR81</accession>
    <name>A0A5B0RR81_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029062</name>
        <name evidence="3" type="ORF">PGTUg99_007139</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000142">
        <property type="protein sequence ID" value="KAA1128320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RR81"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="148" mass="16679" checksum="CE7CABAA3B678B47" modified="2019-11-13" version="1">MKQDSRPALPVLPFATKERPSLGTRQALPSFRSRRKNSTVCGELNQIEKHLAFPFATKALGPPFCDERTALPSFLLRQENLTVCGERNQSKKSSAFPFATKALRSPLLRRENGTTVLPFATTVLPFATRERHYRPSFCDKRTQPFVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1Z0</accession>
    <name>A0A5B0S1Z0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030649</name>
        <name evidence="3" type="ORF">PGTUg99_031565</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1Z0"/>
    <dbReference type="OrthoDB" id="2495945at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366699" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="24871" checksum="7B60C7CFE27313CD" modified="2019-11-13" version="1">MVSLTYLFTLLSVLVAVSRAEDVTTVKNENVDAKWRGGYFGFNSMYNMNSWYAYSGLSMQLMGGSWLNNWAGMLTGCSGGLFFPSAWGMNGFFAKAAEEAHSVSRRAITLDAEQFFRRDQVADSATCLNNKGVHEVYSKNDCKNAAKILHEKNVHTASSGNCQLSLYTAKEKVFAKQLPDQMLMKAADRILSTCGEKATHPKDSNQLHIKDDQVAMLLSRPSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S887</accession>
    <name>A0A5B0S887_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010676</name>
        <name evidence="2" type="ORF">PGT21_011051</name>
        <name evidence="4" type="ORF">PGTUg99_024443</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S887"/>
    <dbReference type="OMA" id="LWIIELH"/>
    <dbReference type="OrthoDB" id="1696280at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000113">
        <property type="entry name" value="Enoyl-CoA hydratase/isomerase family protein (AFU_orthologue AFUA_2G14850)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF75">
        <property type="entry name" value="ENOYL-COA HYDRATASE_ISOMERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1134078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="31977" checksum="4A0F481CE2D4E1F9" modified="2019-11-13" version="1">MASVTPLPSTTRYPCSTLTKPADYPGVFLLELHSPPDNRMTEHFITSSLIAPLLDVEREYRASLLGLLTTPPPGDSQGWKTPLTPGQYALVTCGARDKNRIYSNGLDLNEALALDHFFQHVLNPLYAKLLAFPIPTVAAINGHAFAGGFCLALVHDFRIMKDNEQKGRALMAMNEAEFGAPIPLGLISIVETKFPSTTLAAKCLAEAHRFVAKESLELGIVDALAPESEVVNNSLRLAFEKSIRASTGVYGMIKESVYRKTLETLGSSGSGNADVKSVHTSRLKQLSGHKSGASKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SBY8</accession>
    <name>A0A5B0SBY8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">VPS8_1</name>
        <name evidence="4" type="ORF">PGT21_026321</name>
        <name evidence="5" type="ORF">PGTUg99_011055</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000042">
        <property type="protein sequence ID" value="KAA1135025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059070">
        <property type="entry name" value="TPR_VPS8_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25066">
        <property type="entry name" value="TPR_VPS8_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 8 central" evidence="2">
        <location>
            <begin position="982"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="domain" description="VPS8-like TPR-like repeats" evidence="3">
        <location>
            <begin position="1585"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="241"/>
        </location>
    </feature>
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        <location>
            <begin position="253"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="653"/>
            <end position="676"/>
        </location>
    </feature>
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            <begin position="1773"/>
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        </location>
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        <location>
            <begin position="1882"/>
            <end position="2109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="204"/>
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            <begin position="221"/>
            <end position="241"/>
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    </feature>
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        <location>
            <begin position="2091"/>
            <end position="2108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1093136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1135025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="2140" mass="237887" checksum="5408ED2E384FE5F2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJ98</accession>
    <name>A0A5B0LJ98_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008434</name>
        <name evidence="1" type="ORF">PGTUg99_010247</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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            <dbReference type="EMBL" id="KAA1063960.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
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    </evidence>
    <sequence length="73" mass="8234" checksum="8B045E20B9C0E3AE" modified="2019-11-13" version="1">MRLYVPPDARRGPTNPLKTNAINILNQNRNTIRLNKTYSITMNTTTKPAVRRGGLFKTQSLSFTHKVKGAVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJF6</accession>
    <name>A0A5B0LJF6_PUCGR</name>
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            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
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    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="ICln/Lot5/Saf5"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <end position="45"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1064997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="259" mass="29126" checksum="22520486AED7BA56" modified="2019-11-13" version="1">MTDGTLNESNEMMMIREIEAQAKFRATASSRTSRTSGQGEDGQEQIEDEEIFHSFTPASFDLLVPVLKLKLDSVRPRFENLPAPASCQNRHPKFVESISLLTGWSDLICSSIILTERELSFYSKERDRSIIIPYQAISLHGISGGGNDAQLYCQLDLEEMIGDERMRSEANEQQEGEEESEEPIVLTITPEEGDRSALVQEMFRAMSYCASLHPTSLPRIEDQADKDDHHLLDALLSHDNSPEQPPADHLAAANRFQPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJR2</accession>
    <name>A0A5B0LJR2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024921</name>
        <name evidence="3" type="ORF">PGT21_025030</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJR2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10843" checksum="249D52C1E1393830" modified="2019-11-13" version="1">MANRQPGKRLPYITAEAKSQYIRELFDRALPRPGVTSSLVRHPRVHMFVRRHTPLPSTPLGTPPRSKLSSQGVAIKRRRVVVNCLGPVRRKRRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKF1</accession>
    <name>A0A5B0LKF1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKF1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11132" checksum="0F186AE218A2D891" modified="2019-11-13" version="1">MDVLHEKRVPSKANQLPEELGSMTDIALANHITKRSIDLYQDKPKDLQGALINARLPLPRTPTPPPLQKGRAIEPAPQGGAGLIARPSESCFRGKTGFRGAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LL98</accession>
    <name>A0A5B0LL98_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Succinate dehydrogenase assembly factor 2, mitochondrial</fullName>
            <shortName evidence="3">SDH assembly factor 2</shortName>
            <shortName evidence="3">SDHAF2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">EMI5_1</name>
        <name evidence="4" type="ORF">PGT21_021486</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit of the SDH catalytic dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with the flavoprotein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SDHAF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LL98"/>
    <dbReference type="OrthoDB" id="284292at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.250:FF:000004">
        <property type="entry name" value="Succinate dehydrogenase assembly factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="entry name" value="Flavinator of succinate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03057">
        <property type="entry name" value="SDHAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028882">
        <property type="entry name" value="SDHAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469">
        <property type="entry name" value="PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="YgfY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1065009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="229" mass="26526" checksum="BD7746F01969E437" modified="2019-11-13" version="1">MHRIQVVKRVDRLVCQPFPSTCVGRTHLFKPPVGFYRMKSGASSSSDGKPAEQDEKLHPIFSTNKTAPLPSFKTDPYPIPLQNPAHPTTKTDTTQSYSVNQEIYHQIDQLKQVEDKHAESYDDALSASTIDKLRSRLVYQSRKRGIVEIELLLSTFIEAQRWLKDWDLKQLTQYSRLLMLPDWDLYYYLVKKQEPPSDSEFFKSDLLDQLRIHTSNSSKTVRIMPSLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLK3</accession>
    <name>A0A5B0LLK3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_013797</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLK3"/>
    <dbReference type="OrthoDB" id="2496167at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF458">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="460"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="482"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="520"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="148"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="100"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="125"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1064760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="555" mass="60397" checksum="A6C7AF87E7459182" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LN27</accession>
    <name>A0A5B0LN27_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M50B-like-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_007584</name>
        <name evidence="3" type="ORF">PGT21_032943</name>
        <name evidence="2" type="ORF">PGTUg99_007270</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LN27"/>
    <dbReference type="OrthoDB" id="40823at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049500">
        <property type="entry name" value="Peptidase_M50B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33979">
        <property type="entry name" value="OS02G0221600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33979:SF2">
        <property type="entry name" value="PEPTIDASE M50B-LIKE-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13398">
        <property type="entry name" value="Peptidase_M50B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1118834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="29299" checksum="9AB500EB445CA860" modified="2019-11-13" version="1">MPTLVYDQYQTLHSFQARSVGEALSPNSTQRITLYVIAGYALFILIAWNLWGLRHIIYPFKLLTVAFHEFSHAIVGKCTGARIESITLDPNEGGLTKMRGGIQACTLPAGYLGSSLIGALLIFAGFDTVASKVASIILAVMLLITLWWARNWLTRIVVVIAIGIMVAFWFIDHGSPLRFYVLFNGVMSCLYSVWDIVDDLVFRKVNESDATQFAKLCPIIPSRVWGVIWLLISVIFMLGGILAGLAAFKDSTSEQTSASQKFIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LS29</accession>
    <name>A0A5B0LS29_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_016946</name>
        <name evidence="12" type="ORF">PGTUg99_019957</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LS29"/>
    <dbReference type="OrthoDB" id="542931at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019185">
        <property type="entry name" value="Integral_membrane_SYS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952:SF0">
        <property type="entry name" value="PROTEIN SYS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09801">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="359"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="44"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1067787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1093469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="45077" checksum="25CD3C3987E70F09" modified="2019-11-13" version="1">MRCASGRSAVIVITPGRACCCEPRPGELETQSEVLKCCKLPTNHKKQRTTHQHQRKRKRKKGKRKEKEKERMNSSKNIKETNSETEEELSPIITSYDPTPLLHGGPTTAAAAAAGPTGQAAQQDHHHYHPPAKQGQTNWKSDTRKFRIQGWDPVLIISQILAIQALHYLLLSLITPPLLRAFARPDQLELEGGSQNVAMIIDWRAMSGATTLHSPHAHRLDGAAGFGRLPNSLGRKPFRIGNRLPSLLNHPPNPQHSHLNSSTPDSSADPGLLDKISRGLGPDWVPQNLNYSARHAELYYDSNRSWVISSAWLISSLIGVIILYEIVRRPKQIMDHSLTICLNHLILSTYYSAHLPSSLWFYFVLLLSAVLQILLTEHLCVRREFRDGLGVGWKVDHRPDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVM9</accession>
    <name>A0A5B0LVM9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019186</name>
        <name evidence="3" type="ORF">PGTUg99_003505</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVM9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="118" mass="13984" checksum="F35D312F0DDF74BA" modified="2019-11-13" version="1">MAAKKAVDKEAELTQAAHDRSMQEGQLEIDWELAKKRNEVLDEEQRVRNKDRREEKRLAHELRAEEARRYKDSQKQLAEDRKVQDDARRDQERDARLFQAAMMRMLGVQEPIPRGNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LY41</accession>
    <name>A0A5B0LY41_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_013833</name>
        <name evidence="3" type="ORF">PGTUg99_036957</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LY41"/>
    <dbReference type="OrthoDB" id="432412at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd24163">
        <property type="entry name" value="RWDD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017359">
        <property type="entry name" value="Phi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059181">
        <property type="entry name" value="RWDD2A-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15955">
        <property type="entry name" value="RWD DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15955:SF8">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06544">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="10"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="333" mass="37135" checksum="748AE4439DD21775" modified="2019-11-13" version="1">MAKISQEAKEEIDLLESMYPDNFQSIDDDRLACNDGDHHHKTSFLLNLRPLFGDGLEHQVEMRITIDGEEGYPTSGAVPGVSLGGMATLSREDGERFKQTYQRWIAQFVEQRKAPAEVCLDVLVAKLDELAAGFSPSTSSATTSSGPQLNGPPQHQPLPPTQHEHHNLSMAWAVFWMHHIKATGKRKNIVAWARELGIRGWSKPGYPGALIIDGPRTAVEEYSQRLKGLRWKAIQQRHFEVYQLPVSSAPTTQIVTGSSSSFNQDHQEHEDIERHRRIRKSLPSSSSSNTGGAHSLEDHCPDAVFEVHSMAHLLLLARASGFDDQVLQILKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYM1</accession>
    <name>A0A5B0LYM1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sorbose reductase sou1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_002509</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYM1"/>
    <dbReference type="OrthoDB" id="2499089at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000245">
        <property type="entry name" value="Short chain dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF4">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1068808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="32812" checksum="82DA8423D638BE37" modified="2019-11-13" version="1">MTTASASKSSQTPEVGVAAALTHGCGDRGASKFRTVFDEFRLDGRVGIVTGANGGIGLESALALAELGAKIWVLDVVESPSTEFEACAVYAKALYRESASRRGEDPAKERLVYRPVDVTDQNALQKICQEIVASEGRLDICIAAAGILPAEKSCIDVTSEEFQKVMSVNSTGVFNTATKAAAEMSALGIPGSIILIASMSGSITNREHAWIAYNTSKSAVLQMGRSMACELGSRGIRVNTLSPGHIRTAMTARYLDENPELESKWSSMNPLGRIGRPHELRGVISWLASDASTFCTGSDIIVSGGHHSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZ69</accession>
    <name>A0A5B0LZ69_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZ69"/>
    <dbReference type="OrthoDB" id="10369229at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11962" checksum="AA4BF5F5DABF926D" modified="2019-11-13" version="1">MYESMPSPPDHFQESQIQTAYQETQGSEYTPESQDAESQEFPESIRPLDGSEVYCKFDIDYSIWVNTLENPAKYTHVCPVKPPRQLRMSVTDMSFSQFKSRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1W2</accession>
    <name>A0A5B0M1W2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1W2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="96" mass="10686" checksum="F6981EF50F1E5875" modified="2019-11-13" version="1">MFMSGGKPVTGPLEPAQHYIQKGGRLKSQLSQTSASAHLPTGSVQTQHPSRLRRRNLASTGLPCIDFANPTFYLWKMVNNHRIANADDGRFNKSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7U1</accession>
    <name>A0A5B0M7U1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022975</name>
        <name evidence="1" type="ORF">PGTUg99_016655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7U1"/>
    <dbReference type="OrthoDB" id="10280004at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="55" mass="6151" checksum="34F0EED739AE7152" modified="2019-11-13" version="1">MAKVYAGHLLGEHTACVASNAIDFKSGIYLTRLHIWIIERTIDALSRTSTLNNYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M968</accession>
    <name>A0A5B0M968_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M968"/>
    <dbReference type="OrthoDB" id="2500006at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="336" mass="36920" checksum="A81CB8C1E109E4B5" modified="2019-11-13" version="1">MAYSSDQDGEYELDDSVLAPTPRTDQDHPPIVAGSPNPTGPGYVPNAHIEVGENVGLFNPNDDRVSHVDPLLQLPSEDNDQSDIDSVFSLLDPYEDPTDTTYVPPKKSKRSYSGRKRSSGSLSPKPKPAFVRPRDSKGRFVSLRSKNLGKLKAASVNDQLPQSSSSSTSLPQAVVDSGHPHLTETALNTFLNTVLTSPDEAFPLSFHLPDPFAAHFSLAGPSVLGTNPFIPPTPFIHMRPDDPRLQFRTSFVDLLRVIVPFLSSFTPSPDDHESFKLLNHAVVAAQDWYIRSHHLYHLNAIPKFPSPEIQAHPQPFAEKSKPRSPGSFFDKGFPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MA92</accession>
    <name>A0A5B0MA92_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">acetyl-CoA C-acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ERG10_1</name>
        <name evidence="15" type="ORF">PGT21_002387</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MA92"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000007">
        <property type="entry name" value="acetyl-CoA acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF156">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="13">
        <location>
            <begin position="70"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="14">
        <location>
            <begin position="335"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="11">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1073169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="455" mass="47843" checksum="AF85D301770C6520" modified="2019-11-13" version="1">MTLLLCCIYSKLVYQPAVPGLLLLDSQLLAMPLLPIPSAARRIFQMAISPSLPLNSHGFHSSVASKLPEVFIVAAGRTPVGSMSGSLKALSATELGAQVVKATIDRAGLKPSDVEEVYMGNVLQANLGQSPARQVVIGAGCPESTEATTINKVCASGMKSIILATQNLQTGNRSVMVAGGMESMSNSPFYLPRGSTFGHFQASDSIVKDGLWDAYNQIHMGSCAEETAVKHSIGREAQDDYAIESYSRAKKAWDAGVFKEEIVPVEIKDKRSGKVVLVSEDEEYKNIKLDKVRALRPVFKKENGTVTAANASTLNDGASAVVVMTGDKADSMGIKKLAKIISYADAACAPIDFPIAPTLAIPIALEKAGLKIKDMAKIEINEAFSVVALANLKVLGINSDRVNVYGGAVALGHPIGSSGCRIIVTLAHSLKPGEYGLAGVCNGGGAASAMVIQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB20</accession>
    <name>A0A5B0MB20_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acid phosphatase pho5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB20"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF24">
        <property type="entry name" value="3-PHYTASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022921186" description="Acid phosphatase pho5" evidence="2">
        <location>
            <begin position="26"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="43316" checksum="1A3A7EFBCAE5C753" modified="2019-11-13" version="1">MPTFMALSLLLFAHWNLESQCSAHGQVPFRLTSASDTRSLALPNWIVHHAGPIAPFHQIGTYQSPPKGCKIVQVNQLQRHGSRYPTAQVHKTIHRALKKLKQSSHLDESLDFITDYQYHLGEDSLLPLGAMESFEAGVQFASRYEGLIDRENLPFIRASSSERVVDSAQNFTSGLASWLEININPIEPLVISEDPDSNNTLDNNSCPNRKSSGEKQQWLNIFGPRITERLNSLAINAELNNEDTLALMQLCIFESIADEKLSRLCGIFEHVDWPGYGYYYDLDKYYNHGLGNRLGQAEGISYVAELIARLTGDRKWVEQDESKVRKYQEILSSLSVNSKSIVHFLNLENYHQQGMLTALPLSHTCIKNFRSIKLSTNHGLLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBQ0</accession>
    <name>A0A5B0MBQ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <location>
            <begin position="12"/>
            <end position="22"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1073716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="154" mass="16573" checksum="A7A4E24DDE425A53" modified="2019-11-13" version="1">MLKSYTPGALLAKRDRGTDKTRPIRGTNEISSLFFVVNSTKPSPSRCGAGGLVAHIDSTTADHQSTYNQDPLMWVLTEESVSRLRGPRVEHPPAGSASVHCRSLTPCALLFGAAADSLSAALLASLSAPPDDLGMDPWNDMWSPRKTVDRQELV</sequence>
</entry>
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    <name>A0A5B0MF63_PUCGR</name>
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    </protein>
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    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="1979" mass="214072" checksum="0BFC6CA367578649" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MG11</accession>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
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                <person name="Schwessinger B."/>
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                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="74" mass="8000" checksum="C7404B01A7E07342" modified="2019-11-13" version="1">MCECPSRGAEPELGCNRHLDESACVTAAVGAVTAHETPNETPRCRSARERHAIVRGVMTATVNLGTNSDNRHFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0MGA8_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">eIF2 kinase Gcn2p negative regulator</fullName>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <dbReference type="EMBL" id="VSWC01000157">
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    </dbReference>
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    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="177" mass="20117" checksum="5F94D56F8BF2BD8E" modified="2019-11-13" version="1">MPPPPRDSQEDPEGARILLSFLVDRLSASRRSEPTDELSAMEAILGEESFNCQFLQHEHLQLHFMSSSNWISITISTLLTRTRTLQRMILASNCSSRSQTTIHHKTQLLDSVYWLNIWAHSASMKPYWNRSGRPSKASLPNSLSCTTESRMRERSSPSSIGPVIRIDGAKKPPQRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJX9</accession>
    <name>A0A5B0MJX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034408</name>
        <name evidence="1" type="ORF">PGTUg99_035859</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJX9"/>
    <dbReference type="OrthoDB" id="2509590at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="147" mass="16048" checksum="1055C148B037394E" modified="2019-11-13" version="1">MRASASHRIKRLSHRIGVSDAGPRIASSASEKPMRRIGIALISRCDARYFAGDADIIGRHRLNLGCDSVPEVDSRESSPRVTPDSESNFKFKRHVQPKLNTTSIRWTRRSESDSPQVEHDINSLDSSLGGGLATGIGFGHSIGINLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML37</accession>
    <name>A0A5B0ML37_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal recognition particle receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_003822</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SRP receptor beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML37"/>
    <dbReference type="OrthoDB" id="41266at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019009">
        <property type="entry name" value="SRP_receptor_beta_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09439">
        <property type="entry name" value="SRPRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1077342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="334" mass="37304" checksum="5F6C99E4EC52E54E" modified="2019-11-13" version="1">MDHSLALIIASFTLLLVLLCLKFLFYPSRNKQSDRSAHGQNVQVVISGPMGNGKTHLWSRLIYGTDRIETVTSMVENRITIHFERQLVLDENERQDSAETPAVCLVDTPGHPRLSCRSLARNIPEAKGIIFVIDSQLGLTGKGLRETAGALELVLSFLQLLHCQAPDSKLPKLSIHLSNPARSPTSPPSPSGKSAIVQKTRATLTKELNRRKLNSVGRSNTGPGFRKSRLESLDAIPSAEFTSFFDLIKRLFKFNRSVELTSESTAGPIQLPEEESEVTKLLEEEEDQLTADQKSASPLDRYERFSGHQLHWTVSGQEVENEINLIKKWLIELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MML3</accession>
    <name>A0A5B0MML3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MML3"/>
    <dbReference type="OMA" id="NDHERNG"/>
    <dbReference type="OrthoDB" id="445357at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="36856" checksum="76C3E48BAAB0064F" modified="2019-11-13" version="1">MLMRTCNQTINQQIRLTKTLYHAQHLNRTFASQTLLSRTDFQNKSTVELKEELNKRGIITGSKKKSILIDKILEDEQRRMNSATIRAKKVPPIVRRGEAARARRSSTSTLPEPVVSSPVKVAARLMSEPDGETPKTAQIETGSKSASLASPGITLRSEGHQSQVWPASIELPSTPFEEPLGTMIPFIPDNWSSHKQSHAPQMVDLKPSSAEVSTASHPSTLPQGGPSRVVTDRFSPTTKPGQSSSSSDPAQELTSSNQQSPDQIIFNYLASISLPSSFDFLSSKGPSNHDQAASRHQEHFKFEDRPLSDRERNGLYQILAFFGGSWLVAGLFGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNS6</accession>
    <name>A0A5B0MNS6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNS6"/>
    <dbReference type="OrthoDB" id="10282389at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="12217" checksum="F627019424C01C1B" modified="2019-11-13" version="1">MSQNRCTPLLKDLSHVARRCRGGPSRLGRNSTDPSRLDPRAFVEALACPSKPVANLPRVETRGDAAVRRPRPPTPDGGEQDLDNPDLVFQRDALVANARFELVLRSLSEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRM1</accession>
    <name>A0A5B0MRM1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRM1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="218" mass="24615" checksum="319513A24146466D" modified="2019-11-13" version="1">MDDSGSTNNSGSTDNSGSTDDRGSTEDSRPTDNSRLINNSGSTGNSGLMDNSRPADVVSKLEETLDLDNIQKEAGSTENLSSRKDNLGAEGDWKELPGYPTGRIGFIMLLNHDGTEGYLENDNIWPETWWIVSEDRYHMYLGNITGVCLVEEQKIQHSGRKRQKEEEEFLAELVSKMVGLFGKIDWNAFKWVLWDKQARRGTDYKTFWKDWDYAAIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUR6</accession>
    <name>A0A5B0MUR6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUR6"/>
    <dbReference type="OrthoDB" id="10345842at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="383" mass="39538" checksum="B35405FF47E06C86" modified="2019-11-13" version="1">MHGLGVGFGYRSPSNTSDGPLCEGDQRHNKHQPRHHRKIHITDNMISSRFIQFVAIVFGAYACLDIQAQAVTGPSKPSQPSLNAPPHLGMTGDNKTNNPNGLHTAPRQSQAPSAPFSGAKQPDKNPSKTMNNSQTKPAEMAKPTGLENAKPVGPGPVGKEKAESDEDKIPAGAEGLTKRGGLRDVLWKRGDLPSIQGIGKKPTSLKENNTTKKPDEDPGANTGGGKTATGQSTMARESSGYVAGSSAPDAGKNKSGRTKLMLNGGIGSSEPSDTIPSSEGNASKNKQGGVHAGLNERDGESIQHLIRKRQDAGFYKTKFKAPKLGGGTTSGVAVGTFGGPPVPINGVMGPTGPIPLPTAIVPVTPVGGNGMGGMPPMLGGGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVJ2</accession>
    <name>A0A5B0MVJ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Translation initiation factor eIF3 subunit g</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TIF35_5</name>
        <name evidence="5" type="ORF">PGT21_004555</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVJ2"/>
    <dbReference type="OrthoDB" id="639027at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024675">
        <property type="entry name" value="eIF3g_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12353">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Eukaryotic translation initiation factor 3 subunit G N-terminal" evidence="4">
        <location>
            <begin position="111"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="263"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1080373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="318" mass="34382" checksum="0CAB7A6F88078B31" modified="2019-11-13" version="1">MILCVLGRLGGRAATTNYSPNIIDTSVSHHGHPSTTTAVRTISSQAFGKLSTDLMIWSSFSHFRPGDKNILADELDEDELPPSTEKIDPVTGIKTIVEYKWDETGEKKIKTKGKKSGPDLATTTLGEAIKLKIAVPGLIKEPVVEQTTKATIVPGGKRITCRLCQGDHFTASCPYKDTLGGVLGGDAADQDQQDGPGASQAQTNNAAGGGTGKYVPPSQRDGAKRGESMQKSNRDDLPTAPCYQPVRGRPGKRSVGSLWEIRRISRIYVGKDQETGLCKGFAFVSFEDRMEAERAMKKNQRSTVRSSDFGMTCDSRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXH0</accession>
    <name>A0A5B0MXH0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017812</name>
        <name evidence="3" type="ORF">PGT21_019867</name>
        <name evidence="4" type="ORF">PGTUg99_001937</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000047">
        <property type="protein sequence ID" value="KAA1134648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXH0"/>
    <dbReference type="OrthoDB" id="2504239at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1134648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="215" mass="23526" checksum="A0F2888E82D3D993" modified="2019-11-13" version="1">MENAQKITRSKTGTRKEADTTSQTPAQSTTLDATTLGQDKSTTKFPPSKEVSNLLGRNTTLKEVVDTTSVLPEPSVGDQRSHVWEKIKEAQAAGDAILTKILVAAYKDLEDLATTGSPKLTRSLSALPVLSTVESKKDVPTSATVTELEDNLVYAVGAVTSHQDIGFTPYFDENIRKLKAPLPLTIFDREWQKKAIAAHLTRNHQNRRKTKPTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N238</accession>
    <name>A0A5B0N238_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N238"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="160" mass="17581" checksum="787899A36315A23E" modified="2019-11-13" version="1">MALVVNAGLKELGLESPPPPKLKKAFLGSFPYSNKMPRITEEDKDGNEGDNEDGDSHDGRSDFTDDEKENSDDDNNDNDEDDDDDNDDGTEDEGENRKKDGKKNGKKDGKKDGKKDGEKDGKRDGKKDGNKSSSKANRNDSNDLNELTTANSSEEQPEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N578</accession>
    <name>A0A5B0N578_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_002698</name>
        <name evidence="10" type="ORF">PGTUg99_016628</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000209">
        <property type="protein sequence ID" value="KAA1123369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N578"/>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="459"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="512"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="572"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="113"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="357"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1083654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1123369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="613" mass="67703" checksum="33399E63A7B4BDF7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7U0</accession>
    <name>A0A5B0N7U0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035845</name>
        <name evidence="2" type="ORF">PGT21_037325</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7U0"/>
    <dbReference type="OrthoDB" id="420076at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27450" checksum="89952AF926BF0921" modified="2019-11-13" version="1">MLSTQEVNQLLKKYETTFSVDRVGNPVYRYDTNHVASNAPIEDDSLCAVMTRDQSESDPKSLLKPSAKDMLFFGVFDGHSGWQTSKLLAEQLLPSVNRELHSVFRSEPEYVTAYLAKQLAQAHPDKKATDGAREVQIPKGVMEKIWNIVTWNNRHNRTVIDNLDQDDDVVKLAIQNAFVKLDNQIVGRPLQMLAAYEAQRKSGDKSIPKAYGADVTPQQTASLQTLLPALSGSCALLAYIDTLRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8G7</accession>
    <name>A0A5B0N8G7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nucleosome assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_015938</name>
        <name evidence="5" type="ORF">PGTUg99_013978</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8G7"/>
    <dbReference type="OrthoDB" id="19419at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1084059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1093294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="34441" checksum="B0B9FB7C5D45F846" modified="2019-11-13" version="1">MSATSAPEKTDGDHVEMVAFDPTLERETQLKEAYGQAQFDEVLKLDEELLKAEWEAYNHKVKLLEPIYKKRSEFIKKIPGFWLKAMTNDPNCNHFIDAIDRDALSHLEDFTLEHDPKDARNVTFHFHFAKGNPYFSDRILTKKFTVDLEDESVAVNGSDHKPNPILVEMNKKYDLDRQVKSTPVPIQWTSDEHNLVAKKPSMSIEEFEKEEVQIDFNDTAGSFFNFFGHEEDMYELHTNLLEIHSKALDLYAGVVEAGNHELAYSDEEEDEEDDDPNQVVDLESESNDEPKKKKAKVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9Y8</accession>
    <name>A0A5B0N9Y8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000114">
        <property type="protein sequence ID" value="KAA1084938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N9Y8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="66" mass="7425" checksum="D2A5DDFAAEA476D5" modified="2019-11-13" version="1">MTTSHPSTSSSHHFGPSHLRQPDHPDHLDLQIQSIDQLLHHSQQEAQQQQQPSATTNPWISDNPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDC4</accession>
    <name>A0A5B0NDC4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010805</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDC4"/>
    <dbReference type="OrthoDB" id="2507558at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022830238" evidence="2">
        <location>
            <begin position="19"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="574" mass="61198" checksum="7BE954D1C422D185" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEY0</accession>
    <name>A0A5B0NEY0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEY0"/>
    <dbReference type="OrthoDB" id="10635255at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022876701" evidence="1">
        <location>
            <begin position="21"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="11355" checksum="080B2E6F7A418959" modified="2019-11-13" version="1">MDPQLFTAILATSIPIIILAKHWNEGNPYYSNGGQATYVNFLLHLAQEELFHKVTSMQRDTFMILIATLRSKGLLVDGRLATAEEQVLIILKNCLLQQLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NF46</accession>
    <name>A0A5B0NF46_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zf-C2H2 Zinc finger, C2H2 type, variant 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MSN4_3</name>
        <name evidence="10" type="ORF">PGT21_024667</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NF46"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF32">
        <property type="entry name" value="ZINC FINGER PROTEIN RST2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="598"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="629"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="249"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="458"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="463"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="481"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="506"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1087184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="702" mass="76703" checksum="CE3BCF0F0479F34E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFA1</accession>
    <name>A0A5B0NFA1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFA1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1087234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="71" mass="8006" checksum="9079B635101AF3B4" modified="2019-11-13" version="1">MPQAQIFRRGGGNYTPSKIRYSSKKIRVDLSANMRLELLALTTVIIQQGGVYGHRYIGILAMSYITYLKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFB1</accession>
    <name>A0A5B0NFB1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_032203</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFB1"/>
    <dbReference type="OrthoDB" id="2498341at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051704">
        <property type="entry name" value="FAD_aromatic-hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865:SF2">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="11"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1087473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="427" mass="47543" checksum="90E9D62E954F17BF" modified="2019-11-13" version="1">MAAIRNPTNLRVLISGIGIAGPVAAYWLSKAGVSVTVLERCDCLRKEGQTVDIRNEGVKIMKWMRVEEEVRRRTTKELGIKFVDSNNQTWAAFPQSGEGSFTSEFEIVRGELATIFYEASGDKIEYIFGDSIDSIEETGHSVKVTLQKDSARHMEYDILIVAEGLTSRTRAKAFNEDVRAPIRSLNLWAASFSYKQGATDEDWARWYNIPNRRGFLVRPDGFGRVRASAMCIDDGEAIKAIASSRTSTEKQKEYFINTHKGTGWESDRILEGLRTADDLYVQEIAQTKAKTWSKGRVVLVGDTAFCPSAVTGMGTTAAITGAYVLAAEIVKNPSDHRAAFSAYEGSLRDWIEKVQKLPPGVPNLMHPQTGWGIKCFLGFMFLVSTVVNSRLFALLSKLNPTIYFDITTKTALALPDPSIFEQHRNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFI7</accession>
    <name>A0A5B0NFI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012478</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFI7"/>
    <dbReference type="OrthoDB" id="2509775at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="93" mass="10767" checksum="A658A379615FB936" modified="2019-11-13" version="1">MMEIEPPQVEDTPHQRAVINVQNAARVQEMQAAISNRTQELHILPELVPNVLGIWNMQRMKYGRDPIRIRNIENLTNIPPKSTTTNNQRKIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIZ0</accession>
    <name>A0A5B0NIZ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000098">
        <property type="protein sequence ID" value="KAA1088572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIZ0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1088572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="89" mass="9958" checksum="AE720D7026E4D69C" modified="2019-11-13" version="1">MIKGPRAESFQLEVPPFASPHHNLELKTIQLEVEPLEVKPVEITSTRALELSLFSSRSPPFASPHHDLELKTIQLEVEPVESHHQGTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJV4</accession>
    <name>A0A5B0NJV4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000942</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJV4"/>
    <dbReference type="OrthoDB" id="10554624at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010816">
        <property type="entry name" value="Het-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07217">
        <property type="entry name" value="Het-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1088936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="13560" checksum="362CC1720D4F70F5" modified="2019-11-13" version="1">MLGEASDQILSASIANLTAQLDGAQDENKKPGGINLNLNKSLLSKLPLGGGRSDIQHNLDTMDEKKHAFDLDGRINQLAPAKLQAKIWDLFKVRDNLMQQIEEIIQGIPGLSSMLENLSLSLAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNN5</accession>
    <name>A0A5B0NNN5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017232</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNN5"/>
    <dbReference type="OrthoDB" id="10467086at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1090897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="211" mass="25714" checksum="19FC1DA80F61A10F" modified="2019-11-13" version="1">MRPFHRFIQALDPESQRRLVHDSLEIMMKALEKKFYPRETDTSEFITIYKPFFQKNMYLEKKITNDHFSSMSDSENLETERIEQIGPILQYAYKCIQKPPKNSDRKKSTHEFVFSYYFLNYIDTFDKEAIEKSNVEGINIELLKKQLKLIRDTCRILNDPFDFYEKNQLKLEKKITQYKKNKEMEKWSKEFEEALFSWENMDLFQPLRQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPZ6</accession>
    <name>A0A5B0NPZ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012323</name>
        <name evidence="2" type="ORF">PGTUg99_031509</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPZ6"/>
    <dbReference type="OrthoDB" id="5584477at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248:SF2">
        <property type="entry name" value="FUNK1 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248">
        <property type="entry name" value="FUNK1 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="81" mass="9415" checksum="84999C878C477619" modified="2019-11-13" version="1">MIWICIHFPREERKQTSVTLWNEQIPENLGSIKKDLLSEPSRLTNHFTSRYKQSQPLIQLGESSASHQPNLRWSEQGVGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQY4</accession>
    <name>A0A5B0NQY4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022683</name>
        <name evidence="4" type="ORF">PGTUg99_017126</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000178">
        <property type="protein sequence ID" value="KAA1125457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQY4"/>
    <dbReference type="OrthoDB" id="2509717at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137548" evidence="2">
        <location>
            <begin position="25"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1125457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="186" mass="20621" checksum="981143692AB1E306" modified="2019-11-13" version="1">MYPLMNYVTCLSVVASVLIPTTLGQPVDRQVIWLTEEPLHGCQIVTIDPTTRFPWKPENEAVNNPFAPGSSNAAETSSSTARPDPTPLFNFVGMESAFPNDPRPDTVRIHNHSGQDLWVWTRAATVKERVNANCERSIRLDLSFPYLLAAPCGIDRRVLQHSTTWKIVANELGLEQKTSGSSCNCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NU39</accession>
    <name>A0A5B0NU39_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003813</name>
        <name evidence="3" type="ORF">PGTUg99_019677</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000312">
        <property type="protein sequence ID" value="KAA1105158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NU39"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137568" evidence="1">
        <location>
            <begin position="33"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="121" mass="13070" checksum="B5C3C497B709D59A" modified="2019-11-13" version="1">MKLKAPTLVSVYAGALCLLLCAVGGLRPETFACATCGSAMPVIPDHRLCGQPLFCETHGRQTGDACGFLNMGDYVACGRCNFRSTTTWYQQCNSDHTTSHNDGCDSVMDPMVLTLQDQDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUI1</accession>
    <name>A0A5B0NUI1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034854</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUI1"/>
    <dbReference type="OrthoDB" id="10445690at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="375" mass="42267" checksum="26AEFC31F0F62492" modified="2019-11-13" version="1">MLWYTTAWLVKSLWPPEVFIGHHYPWQDCESHPVNFYFFILKGKNINTRSGVDSKGQETQCMAASINVCDNIYTVQPVNQNATSSLADDTGVAVVPGPFRWTVWSVVPTPNKQPAAYSSLSNLGFQYMEESLSCNITDARFTYYLKPRPKFKKSAGVATRTFFKTQFICAVISIHQPRAYNQSPNNADSSGILISLPKFASKFYGRLEQFVFSSARRLSFEKFNLDNVQSLIPSDAPKRLESTDITQLEISLKKMAFVPSPKSTLNTPASIRNYQMEGSLYLGNTSELTDPWPAFMNIKFIESTFFIITFKEDIEVRLIMNESNLLKMPSALSLLFSEQLEILSWLVDSTGSDLHNRSFGLQTQLVLLALACGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NXJ9</accession>
    <name>A0A5B0NXJ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005829</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NXJ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1092518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10495" checksum="87C5DCDDA8DE7486" modified="2019-11-13" version="1">MALCSSNSLCLARAQLRVSPLVPLQIKNLYGAPSALSKVKSDRFLIMKLILALSALYAAKSDQSWTRSRLVVLGPLPSNQRREQSGLYPLNQQHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NXV9</accession>
    <name>A0A5B0NXV9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">snRNA-activating protein complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_008938</name>
        <name evidence="9" type="ORF">PGTUg99_023721</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNAPC3/SRD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NXV9"/>
    <dbReference type="OrthoDB" id="3437960at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003681">
        <property type="term" value="F:bent DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001046">
        <property type="term" value="F:core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022042">
        <property type="entry name" value="snRNA-activating_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13421">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13421:SF16">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12251">
        <property type="entry name" value="SNAPC3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="154"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="489"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="611"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="177"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="501"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="519"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="617"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1092628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1093724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="665" mass="73441" checksum="DDE992867EBB14A6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZ45</accession>
    <name>A0A5B0NZ45_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_025453</name>
        <name evidence="5" type="ORF">PGTUg99_022415</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0507 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2536252at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097422">
        <property type="term" value="C:tubular endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051248">
        <property type="entry name" value="UPF0507/Ank_repeat_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24170">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24170:SF1">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G09870)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="3">
        <location>
            <begin position="1088"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="299"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="521"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="615"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="716"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="856"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1308"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1469"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1643"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1687"/>
            <end position="1837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1872"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="378"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="521"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="571"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="740"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="857"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="902"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="917"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="933"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1309"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1321"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1473"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1489"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1647"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1699"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1733"/>
            <end position="1759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1760"/>
            <end position="1771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1782"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1816"/>
            <end position="1837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1878"/>
            <end position="1903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1094577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1129009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1903" mass="207018" checksum="51851D8A3511492C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZS0</accession>
    <name>A0A5B0NZS0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020405</name>
        <name evidence="2" type="ORF">PGTUg99_005726</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000479">
        <property type="protein sequence ID" value="KAA1071156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1094413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZS0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="118" mass="12812" checksum="46C662C0BFD0C304" modified="2019-11-13" version="1">MFMSGGEPVTGPLEPARHYIWGPEVGQLKKPTQPNISIGRHLPLTGQPTSIPTSKSHDRSPTRKPFQTSRLDQSVPSTGTQPTQFILQGVEPRGSRPKTSPRRTLSTSSKLDGRLGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P271</accession>
    <name>A0A5B0P271_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGTUg99_026070</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P271"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        </location>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="555" mass="62287" checksum="4EFEFF382336B598" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7X9</accession>
    <name>A0A5B0P7X9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7X9"/>
    <dbReference type="OrthoDB" id="10413005at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="141" mass="15847" checksum="FB95C0AF9ADE5B46" modified="2019-11-13" version="1">MAQFRPRQAPEPLPETPNPPTLTSLSQQLIREHQFLAHEAQSTNILRTATAARNHMEVMRSSNQLLAAQDPPAQLAPATVAAMEAMFARFERRQEERFTRFERQLQGQLDLITNQLGLLNQDLASLRQGLARAAAPQDQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P988</accession>
    <name>A0A5B0P988_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013947</name>
        <name evidence="2" type="ORF">PGTUg99_009962</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1133996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P988"/>
    <dbReference type="OrthoDB" id="10461387at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1133996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="155" mass="17990" checksum="1811E48D98F3E087" modified="2019-11-13" version="1">MKNHRRRFQLEDLPHQNLHKDNIDLFRSIVFPSYLWQKKTFYPILAQSSQSPRRNRTIRTIDHEDLRLVYLLASGYLLPSELFIDIEPTFRFLPSSTSVCIGELALKVVDEAHVKINNGDFIICCLKLVLRSFSTGSLSMELFSSAEERTCLDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAE5</accession>
    <name>A0A5B0PAE5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAE5"/>
    <dbReference type="OrthoDB" id="10458083at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022828767" evidence="1">
        <location>
            <begin position="19"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="566" mass="66750" checksum="7697C05F9BD08FD7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PC03</accession>
    <name>A0A5B0PC03_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Vacuolar protein sorting-associated protein 53</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">VPS53_6</name>
        <name evidence="10" type="ORF">PGT21_029265</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VPS53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PC03"/>
    <dbReference type="OrthoDB" id="10261632at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.110:FF:000004">
        <property type="entry name" value="Vacuolar-sorting protein 53 long isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.110">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 53, C-terminus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039766">
        <property type="entry name" value="Vps53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031745">
        <property type="entry name" value="Vps53_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038260">
        <property type="entry name" value="Vps53_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007234">
        <property type="entry name" value="Vps53_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16854">
        <property type="entry name" value="VPS53_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04100">
        <property type="entry name" value="Vps53_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps53 N-terminal" evidence="8">
        <location>
            <begin position="2"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Vps53 C-terminal" evidence="9">
        <location>
            <begin position="351"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="472"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1098140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="555" mass="61139" checksum="15DC9DECB1187262" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PD89</accession>
    <name>A0A5B0PD89_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PD89"/>
    <dbReference type="OrthoDB" id="10345143at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="464" mass="51015" checksum="2A4420F2B26C3AFF" modified="2019-11-13" version="1">MEPGIQPLQCDDSADASLPTYKQVITDHPSAKNSVRSAIKKKPRAAQKKKSRANKKAASPEVISDEEKENPAQKVMVSTSFKLFVLDVDKSKKAKKVWLLISPPNPIELEITASPSELATSFDEFKTLVTTNCESAVNNTGRILTEGLLTGSPEINWFVSIARVEGFKKGKPFPLTNAAAFNSWIEALAEVNADESCLSIEMTNPNKVAKLQHDAEVLAKDAARKEAKRLIIAAREKRALEGDTNVGKRKRLDEDSDEEDDDSGNEVDYDKDAVKLIMRQLYATHSANALYDRHLPVYIHPTNRSKFFLLSNGTCQKWARAMAKVTAGVSLQSPPKDIKFDILPSTKAGAGGRTGTGHPTSTPCDHISHACAHRASPQPSSDGPDPGKDVGIRDYVQFIGFRNTDEVVDLLVQNDLQNYKIFRSSNLDRAALRALGLRLGVVTQLCDSVSKFERYLAKQDHMSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PDZ4</accession>
    <name>A0A5B0PDZ4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HIT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_010898</name>
        <name evidence="10" type="ORF">PGTUg99_018450</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for box C/D snoRNAs accumulation involved in snoRNA processing, snoRNA transport to the nucleolus and ribosome biogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BCD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000380">
        <property type="protein sequence ID" value="KAA1091949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PDZ4"/>
    <dbReference type="OrthoDB" id="272357at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070761">
        <property type="term" value="C:pre-snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048254">
        <property type="term" value="P:snoRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23023">
        <property type="entry name" value="zf-HIT_BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051639">
        <property type="entry name" value="BCD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057721">
        <property type="entry name" value="BCD1_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483:SF3">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25790">
        <property type="entry name" value="BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="9">
        <location>
            <begin position="24"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="175"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="255"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="394"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="182"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="427"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1091949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1099531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="476" mass="52600" checksum="7E3646145698CC1F" modified="2019-11-13" version="1">MEASPSTSPLTPAQERPPARSKTCEHCQTTGSKYTCPGCGTRTCSMDCSNGHKASKNCSGKRNRVEYVPLNQYSWGRLMQDYSYLEEIHRHLSHHPPRSSVSNLPKPVGAPLGLTPPSRNSIAKRQLLVRMAALEAVRLLLMPDGMSRRTMNMTNYNHKKKCMQWSVEIVFPSDEQAEEAVPQPTSQDRSSSSSTLTPPPPPTTTTTRTPTVTRHQHTSHLIHQISSDTTIIELVRRTLNLKKLQMSPARKRRLLELVGREEQREKAKQDTRDETGEGGGSKQEGGSKQEGGSKQEGGPKSKVEEAKDGEDLIFMLRLERVEPNVGETEEEGGAEESERDGAGAVRKLGPMERLRTALEGLEVVEWPRLEVIEASEWTRGLEAGRLRECVRIIPPRPTRPSSTTTGLKRPVDSGWPAQKRTRTTAPDIPPSITAPPLPTSLNPSPLPPLIPHPPPSIPSLSSLLHHYSSSEDDQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEC4</accession>
    <name>A0A5B0PEC4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007375</name>
        <name evidence="2" type="ORF">PGTUg99_013290</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000210">
        <property type="protein sequence ID" value="KAA1123234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEC4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1123234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="97" mass="10354" checksum="033FD87573F5F960" modified="2019-11-13" version="1">MPVTEPTGFGSGTSKTRDPILAAGICEVVPRETSLASRCAPGSLPRLPLERLHIYSFPVPPSHVGKGHFHSRPNLTLVPFPFSPGMALELPTPSKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PF44</accession>
    <name>A0A5B0PF44_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023403</name>
        <name evidence="3" type="ORF">PGTUg99_022885</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000208">
        <property type="protein sequence ID" value="KAA1123512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PF44"/>
    <dbReference type="OrthoDB" id="10303905at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="122" mass="13881" checksum="5B45E7F7ADB561FB" modified="2019-11-13" version="1">MQRSSDGVSKLASGDDRTLSKLYNIPPPVRQQLQDMVQPGAGVSPNTLDKIKEHLATIIRTQQETVQCIDDLENAVLGLLRLFAPTKDLLARFYEDIQARPQAPLQQPRQQRHHPHNMRNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PG15</accession>
    <name>A0A5B0PG15_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PG15"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="258" mass="27150" checksum="2490D81945C5A8B9" modified="2019-11-13" version="1">MGFGLDHFDQDTIADEVRPVGRMQSRRLLLFRRQAGLPGGGTDTSLPTTDPKPPTKTPKSPGKSTSSGTPGVAPKTKGSTKPDTSPPRDDPTGNNPVDPSTPPTTPSTPSSADVSNSPPLSNGGKAPQTDKPKKTPEEKEQERLQKITKQKAKIAELKKTLQKEEAKLKKLESDGKKGKNGAEGSQDTPKKEKAGSMGSSPDKQPPKDKAAGPDSTPKKPSNKEKVAGSPGMSPDNQPSFGSNLIASGKSRRAGRVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PG52</accession>
    <name>A0A5B0PG52_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028675</name>
        <name evidence="2" type="ORF">PGTUg99_015739</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000144">
        <property type="protein sequence ID" value="KAA1128145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PG52"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1100063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1128145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="64" mass="7246" checksum="85B98BAFA5A0E6D0" modified="2019-11-13" version="1">MLLQAPGSYGSTHLALVFQTSVDIDFCPNHLISSLIFDYWVIEYGTPCLIVPLNTAQENEFHFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGN8</accession>
    <name>A0A5B0PGN8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGN8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="172" mass="18579" checksum="3E6F47D9A0DB20D7" modified="2019-11-13" version="1">MTEREDGEHMIDLIGSFEITPNTTLTLRVTVITSHFHGAPLSETVHHRNSAKHDPSSIHTRYFNLLSSTLSRPRLMKDLAYLLANMGLLLAPLGIKVDAALTGASKCSVPKCRGSIRLGTFPVEEKCSGCGLWVGATAPVCGSCLKTYAEKVLDACSYCNSRGQKLPSKSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHW7</accession>
    <name>A0A5B0PHW7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031363</name>
        <name evidence="3" type="ORF">PGTUg99_015618</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHW7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="72" mass="7334" checksum="4F4F0E01A32D1BC5" modified="2019-11-13" version="1">MMFSSLLPTTTSIATNSIQTSLPAGNNNNPASSYINIVRSSGSSLLVVGTASSSSERPPSHRNGLQVVGIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKA4</accession>
    <name>A0A5B0PKA4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKA4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="74" mass="8587" checksum="0779CC2951E5E10C" modified="2019-11-13" version="1">MFATSTATFDELSSHLRLWRRDLDRVSFSFIKVIRVLIAHSSKKKMKNQNKKTKQTPMGRGQMKTDLDTVDEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRX5</accession>
    <name>A0A5B0PRX5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRX5"/>
    <dbReference type="OrthoDB" id="2514177at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="357" mass="39568" checksum="F2C0B3DD0CFFA26D" modified="2019-11-13" version="1">MSDYTSSAEAVLKLYEILDDFASFGLDINRETIGGLVLQSSLEIGSEWRKEVDRWVEQDLGGSSRTLSKKAVTPDQILKHVDIVKRQLDIGSSGNTFSAMIPQANQARQDDVPDPTMEFYHPEAWPSLPPTVEGLAIRGLQCWNCRSPDHLLSKCKMPRRRNFEQPPPTLEQFRRPWSSTATARPNNPPVLAPGNFQAWYPIVTPPGYSSQGYKQQSSPYAPPTANSGGQSWNRRPDLYRPDNSQRTGPPPSQRPPAQTTANAANTAGNVHQPNQFTNTTATLSSEQQPNFQDSTSRMIEIEVFDENLEHGLVPGFSHLAVGEPDDPVVDTGATHHLTANRGWGPLFSDPNWANRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUP7</accession>
    <name>A0A5B0PUP7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_024580</name>
        <name evidence="3" type="ORF">PGTUg99_023492</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUP7"/>
    <dbReference type="OrthoDB" id="2496355at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137814" evidence="2">
        <location>
            <begin position="27"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="633" mass="72199" checksum="C75CBB2FE27DD18B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PV06</accession>
    <name>A0A5B0PV06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_028172</name>
        <name evidence="12" type="ORF">PGTUg99_013199</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="12 15">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000349">
        <property type="protein sequence ID" value="KAA1097252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PV06"/>
    <dbReference type="OrthoDB" id="269120at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000132">
        <property type="entry name" value="Solute carrier family 25, member 33/36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF4">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="40"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="172"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="315"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1097252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1104612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="410" mass="44207" checksum="2FE5171837BF6789" modified="2019-11-13" version="1">MNNSSSSSKLNEPEPSSSSSSATTTTTSSSSSSNKKILNPPGWVHFVAGGLGGMCGAIVTAPFDLVKTRLQSSTYHADRSQIPRFTFSSSSSAIPTTSTTTTTAAPNRLFYHFIDTGRIIRDIHRLEGFQALFRGLGPTLVGAIPARAINFYVYGMGKEFYCRLISPTSDNSSTLVHICSAITAGIATSTATNPIWVVKTRLQLDVPSSNPPSRSTVRTAGAIAIDLPTRPSINSSILPQLPNSSNLSNRLLNPLGNSLQCISKIYRQEGIPGFYRGLSASYLGVTEGTIQWTLYEKFKRLGSHFIADQDNRQEGTWSTKMAAAGAAKLIATGITYPHEVVRTRMRQKPPPLPAKSKYVGLVSTFRVILAEEGLKAFYGGLSPHLLRVVPNAAVMYTVYEAALSISRSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYL1</accession>
    <name>A0A5B0PYL1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Heme haloperoxidase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_025664</name>
        <name evidence="12" type="ORF">PGTUg99_020860</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the chloroperoxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1120936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYL1"/>
    <dbReference type="OrthoDB" id="407298at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.489.10">
        <property type="entry name" value="Chloroperoxidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000028">
        <property type="entry name" value="Chloroperoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036851">
        <property type="entry name" value="Chloroperoxidase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33577:SF18">
        <property type="entry name" value="HEME HALOPEROXIDASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33577">
        <property type="entry name" value="STERIGMATOCYSTIN BIOSYNTHESIS PEROXIDASE STCC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01328">
        <property type="entry name" value="Peroxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47571">
        <property type="entry name" value="Cloroperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51405">
        <property type="entry name" value="HEME_HALOPEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Heme haloperoxidase family profile" evidence="10">
        <location>
            <begin position="132"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="349"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1105992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1120936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="417" mass="48421" checksum="D01D20079A759B86" modified="2019-11-13" version="1">MAEYKKSNIRSHSKPYRAPLLDFRSLYPFSDDFDPGALWGLRRLIRKGAIELVKLYFFIIFLTIVDLFFVFFNLISPNRTPENVIPLPFSPHSPPRFFDHPFIFLTCKLALWLGPQAPQWIQEWSYWDGLLSDGEWPPIDGDIDVQTVSRSPCPGLNALANHGIINKSGETLNFHRIASRVSRTYNLSPALAVQLLSAAWPLFKDRGQIDLYSLTTHGLIEHDASLLRPDLNESTLEDQTKPNQELIEKHFPKRRVRKTRSRIQKGDESEEEEEIEEETDEIRLRHADFSNLLEKRREECKTNNPSFHETFLFKFFGSGNCAMFMEVFGGKLSDIRPFVGAGISSNPPSHHHHHHHHHHHRHRLHDEEQLRRPAGLSDLINLEVLPHGRWRPSNLHHPWGLTIFKLIYSTLKIEART</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1C2</accession>
    <name>A0A5B0Q1C2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cell division control protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CDC10_2</name>
        <name evidence="7" type="synonym">CDC10_1</name>
        <name evidence="7" type="ORF">PGT21_033388</name>
        <name evidence="8" type="ORF">PGT21_034444</name>
        <name evidence="9" type="ORF">PGTUg99_022759</name>
        <name evidence="10" type="ORF">PGTUg99_023678</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000308">
        <property type="protein sequence ID" value="KAA1107011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1C2"/>
    <dbReference type="OMA" id="QCEFVYL"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032161">
        <property type="term" value="C:cleavage apparatus septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043934">
        <property type="term" value="P:sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000260">
        <property type="entry name" value="Cell division control 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="6">
        <location>
            <begin position="29"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1072373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1078390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1086398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1107011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="318" mass="36418" checksum="D52F1BFC89D9D692" modified="2019-11-13" version="1">MADEAITRLNSYVGFDSITRQIEHKLLKRGFQFNAMVVGQTGLGKSSLINTIFASHLIDSKGRLDADEPIRQTTEIQAVSHVVVENNVKLRLNIVDTPGYGDLVNNDGCWEPIIKYIKDQHSAYLRKELTAQRDKYIQDTRIHCCIYFISPTGHGLRPIDIIVMKKLSEVVNIVPVIAKSDSLTLEEREAFKVRIRDELHYHNIRLYPFDNEEQDAEELQLNEAIRNMIPFAVVGSERNVIVDGKPVRGRKNRWGVINVENEQHCEFNYLRNFLTRTHLQDLVETTAQIHYEAFRSKQLLALKESSANKAHQPQSNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6W3</accession>
    <name>A0A5B0Q6W3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6W3"/>
    <dbReference type="OrthoDB" id="2505697at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="271"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="515" mass="57380" checksum="F2895CD2017C0D55" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAM0</accession>
    <name>A0A5B0QAM0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012627</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAM0"/>
    <dbReference type="OrthoDB" id="2515722at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022859545" evidence="2">
        <location>
            <begin position="28"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="402" mass="41092" checksum="B0B17DFB186C122E" modified="2019-11-13" version="1">MNTHIISRRSFFFVIFFFHIASNSVTTFQTGKSKTTGRLQLRSMAAKAHTPIGENRKLTKRTFGGKFDGPPISKDSGPPSPKGPGPPGPGSGGGDLSLSGAGLGGKNDTGLGSNKTPALGLGGDKDANLPGGKDKGLVPDDKDKGLPGPLSTPPFSPDSGSPSKPGPSAKSALPPKSGSGFGSESSTSSLSEKIESKTIRVEGGKGKDCDKKKQHRPSEKSKDDSEINVSGPTVIKIEVSNNGCSTCCKTCSQEDKKADATDAPPKKKTPEKPPPDISSGKGGDTPPPEITSGKGGDKPPSPSPSPPPDKSKTPSPPPSTPPPSITSPSSDQPNPTIKTPPPSPSPKPSPPPSPPPSPAPKPAPSPPPPGDKPGSGHRHITLPSLATISITLLLWSCLLQNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAW6</accession>
    <name>A0A5B0QAW6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAW6"/>
    <dbReference type="OrthoDB" id="10477702at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="173" mass="19129" checksum="035C699907D1AFA2" modified="2019-11-13" version="1">MFMAVTSARKAAQEADSESRQEETSRSLVNDNVQPRRAARSRAQAPIATLEHWNAPLPANSDPAKKKLPFLTSEAKQSTADDLWARAFSRPTSHPTGTRHPRVYTLIRQRNSVPSPQRGSFSTPASASRGVSSLRSSWPPQPPPSPHPMSQIGSARHDRRTDQMTPNADSRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB27</accession>
    <name>A0A5B0QB27_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QB27"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="121"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="189" mass="22177" checksum="2B71D8C7903C6D41" modified="2019-11-13" version="1">MGKNKRHNNASRKAAKKRCKAKKKQETREQLLKRTLNPSDTTHFVNWRRVKYEELDLYPTIPVDRHKKPTRPPTPEEIKSAYDQVETFHLFKTGRNIVQDPLDKESVIAFIDFIKFEDMSEQDKADLNLFTTFLHRSKKFISPVAVNSRSWGGLMWVIGWRKSSDGDQIISLDIISNKWLIPHLKKTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QH70</accession>
    <name>A0A5B0QH70_PUCGR</name>
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        <recommendedName>
            <fullName evidence="2">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002225</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QH70"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102:SF2">
        <property type="entry name" value="GAF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102">
        <property type="entry name" value="SLR1143 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="302"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="709" mass="78671" checksum="19A59D967F6E08CF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHP8</accession>
    <name>A0A5B0QHP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHP8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10884" checksum="917D4D805923E98F" modified="2019-11-13" version="1">MTARSPISIPSDTSSDNGLRLTGEDLAISMLLTPDGALTYGHDTFTQAEFECLETKILERIQIFCRTFVPRDEEEHRGRIKLYKDLKRYFELGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIL9</accession>
    <name>A0A5B0QIL9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIL9"/>
    <dbReference type="OrthoDB" id="10295524at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="37"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="600" mass="66637" checksum="FDD5E9A18439E59C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJJ9</accession>
    <name>A0A5B0QJJ9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription regulator Rua1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027529</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJJ9"/>
    <dbReference type="OrthoDB" id="2507354at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028012">
        <property type="entry name" value="Rua1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28125">
        <property type="entry name" value="MEIOTIC EXPRESSION UP-REGULATED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28125:SF3">
        <property type="entry name" value="TRANSCRIPTION REGULATOR RUA1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14616">
        <property type="entry name" value="Rua1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator Rua1 C-terminal" evidence="2">
        <location>
            <begin position="842"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="764"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="501"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="741"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1038" mass="113316" checksum="4BDEE84F8F141BA9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QK46</accession>
    <name>A0A5B0QK46_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QK46"/>
    <dbReference type="OrthoDB" id="416093at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="182" mass="19503" checksum="9F1D8838C0AB3F37" modified="2019-11-13" version="1">MGVDKRLSLSFLLADQQLISRCPKSGAVTTMVLITPLPIEDAEQSWTIHPHFSLPLLSLVVAHLGNTSALLCSAIDGKVLPLTKYHHPYSRVESDRLHRIGTGLITDSFGKSRWGTSSSGGTPPGSVASLGTRYPLGYPDICQVWAEKQPSTTKSAPAGGYPLALYPSADSGYPQRIIPAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNB3</accession>
    <name>A0A5B0QNB3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNB3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="94" mass="10478" checksum="B14920CA1B76845F" modified="2019-11-13" version="1">MRFLVVQWLALVYKLPIKIIMRSILGLPIISLFLALQHQALVKSEVIVWQADTLCTPNGIPIQNTHCCTQQILGKTKAQQIPGPCPKGKMVSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPP6</accession>
    <name>A0A5B0QPP6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_031828</name>
        <name evidence="11" type="ORF">PGT21_033665</name>
        <name evidence="10" type="ORF">PGTUg99_026954</name>
        <name evidence="12" type="ORF">PGTUg99_036091</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000169">
        <property type="protein sequence ID" value="KAA1127393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPP6"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF20">
        <property type="entry name" value="AAT FAMILY AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="8">
        <location>
            <begin position="44"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1106251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1109302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1115221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1127393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="540" mass="58551" checksum="E186BF807D056241" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSJ1</accession>
    <name>A0A5B0QSJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSJ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="81" mass="9242" checksum="D2FAB9B8D13D235E" modified="2019-11-13" version="1">MKNAKISIDPHLKQDNKASGSSIPDKEQIIQQYISTYPELQYQYVAASKDEPQEIRESSEGKRSRTQTGFGENFSIHINLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSX5</accession>
    <name>A0A5B0QSX5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSX5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="57" mass="6393" checksum="855ACE755B76A76B" modified="2019-11-13" version="1">MHTFVSYQATHACHPRSFKPLKGRQQIHITLSRKPPFITATEDNSALSPSYLSAKVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTD6</accession>
    <name>A0A5B0QTD6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTD6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="468" mass="51686" checksum="353AB6DEBEC5A5EB" modified="2019-11-13" version="1">MDASSSGTAACNGSFGGPFTSSLARDYHSRCFDNFNPSSPPNQERKSLASRLTSNARSSTHLDRSTQHSRYSQALTSNYLKLPTKLNLDMSPSQPSDALAEALLDLSRKCQELRQQKGHKHEGKATDLKIRQDSLSQIQTTLLPAARNSVVELYKALDLALKKSASPQPQYNDALAIVPSLSKALVNLSLSANNLDPKDEKFGLNDQNSGPLKRFRIENLQQEISELISIFLSDVFEEISELLSSGDFTNSGHDGEPAQPHASIDMVEDSFNRIIHLVDLSDFGLLQSLWQENEEEVGHLLVIINREISIAQHPDLLDDQGFSHKVTPRLVKLLEQCNPIVKSCRGLYQKIASTNLFAISEKLPTVELESIVHHSAGIKQSLQHIVHGLSSAAALQRVEQVRGTQSHLNQLKKSVDDTIESIASHMTPSDPTAKVDEVMDKSFGHFKSTFYPAISQITADFSQFESSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTK5</accession>
    <name>A0A5B0QTK5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_019095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTK5"/>
    <dbReference type="OrthoDB" id="2508371at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023104917" evidence="2">
        <location>
            <begin position="24"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="412" mass="46122" checksum="E45EBE83A8814036" modified="2019-11-13" version="1">MGVLLQMICSLWTHCAISPVVEGMHTTNPVKSIPNIVNVEFPSGTAANTPQEIWPLSNIPGEMLDPQLQATRRTSNIDTIAAVDQNHKQLFNQVSFIADELLRSPVRDEGPEQDIRIPERCLGEFRMENSTRPAKRLKSGRDPQHCASTGPAVRAMYDGFAECLNEPGKIQYRVGPGQARSGFLGNVPVPRRGSQALQQSTPENQSSKSIVEVRAQEGMQGNLSNDHRPPDSSPLESQNPVEILKESHFKKRPIPLFSFPTLESCSSKQALSHPSNDFKQLPPTIQPNPTAVGHPSLLAFGYYSHQALAWFPGQVSQIASLGAHALAYQLLQHIADLDTKAKLQKMHESNGILIPFIYNLILKELSNTHWVRIKEVWGYLWQHWSQLKNVDSDEEVLRTEKRLFPTTIFRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QUF6</accession>
    <name>A0A5B0QUF6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QUF6"/>
    <dbReference type="OrthoDB" id="2509591at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022976727" evidence="1">
        <location>
            <begin position="25"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="540" mass="62735" checksum="6DB5673DD9E7EB1E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QUP8</accession>
    <name>A0A5B0QUP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QUP8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="84" mass="9717" checksum="89FBC47BE023C086" modified="2019-11-13" version="1">MFEFIECLPLDKTTHKPVYGQELMIVRPVEYTIIPNFDYIQAVDNSGKYWGCPSNKNKNFLTCNYCAKYKPSPKKPSPKKPSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QUS9</accession>
    <name>A0A5B0QUS9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">6-phosphogluconolactonase</fullName>
            <shortName evidence="6">6PGL</shortName>
            <ecNumber evidence="4 6">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SOL1_2</name>
        <name evidence="8" type="ORF">PGT21_015615</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="4 6"/>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QUS9"/>
    <dbReference type="OrthoDB" id="432544at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00409"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000005">
        <property type="entry name" value="6-phosphogluconolactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="19"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1116484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="261" mass="28832" checksum="4CF8DC1E6457866C" modified="2019-11-13" version="1">MTPTPSTTPHPAILYALPKDKVARSLGDFVIKAQEEALSKRSKFTLAVSGGSLAKTLVEGLTGRAELKWEKWVVFLADERVVPLDHEDSNYRIVHEGLFSQVPIPTENIHPISTDHLDDPEEVAHDYEKQLMNEFAGKNAVAFPRFDLILLGTGPDGHTCSLFPGHELLKEDSTWVSWLNDSPKPPSTRITLTYPVLNHAHKVAFVAVGDSKKGVIHNVLDLPELGLPSSLVKPIPPGQVFWFVDEAAASLTQYVKTDFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QW72</accession>
    <name>A0A5B0QW72_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QW72"/>
    <dbReference type="OrthoDB" id="10341260at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022888793" description="Secreted protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16231" checksum="EB29ED2683D0F30E" modified="2019-11-13" version="1">MFKAKASLLTAYVVCVIALLSSSVQLSEVPKSLLPRAGTMQQCSYGLESSCYVTEDRLFKCGQCYTGPSRSRAPLSKMTWNDCYRIPTPNLGAQSHVKVTQTNFFHTKDANTLEVGGRDASTNQDHTYECPKAANPQRPYCFDCSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX08</accession>
    <name>A0A5B0QX08_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_007653</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QX08"/>
    <dbReference type="OrthoDB" id="2498342at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000064">
        <property type="entry name" value="Early growth response protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="247"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="83"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="195"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1117443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="335" mass="38060" checksum="D64B18786D2E1C21" modified="2019-11-13" version="1">MMNRPNLPPLRLALAQCDDRDHSNRDTPHRSGYSTRLHSDLSFYSLASSSTPPTSSSSSHIPSPKRDLLPSFMIENLGFSSLKPSINHSERRSSGSAYRTSGSRQHNYIELKDNLGHDREVQPERKSTRGISPWEKSNPQFFRSYRTTQSENPGVPHDKPMSPYHSECHTVSGGIPFNHYERPELEVNKTSKAINSQLPPNLSDQSANLEEPQYGCADPGGRPTQEGPEISPSYRVQPTETASGTRYFCPVWGCGKSFTRNFNLTQHISAIHKDERRFQCTYCMALFYRRNDLTRHERAHTGVRPYQCECGQNFTRTDLLARHKHSGNCAYKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXK0</accession>
    <name>A0A5B0QXK0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PQ loop repeat-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_029268</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-histidine(out) + L-arginine(in) = L-histidine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71063"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the laat-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXK0"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000009">
        <property type="entry name" value="PQ loop repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000028">
        <property type="entry name" value="Vacuolar membrane protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF34">
        <property type="entry name" value="LYSOSOMAL AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="328"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1117988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="51478" checksum="82234DBFADEFE077" modified="2019-11-13" version="1">MLVSDIFGNLSQLFWLFAQLPQIWTNYRNKSVEGLSLTFLLNWLSGDVTNLIGCLLTNQLVFQRNLAIYFNLIDLILIGQFALYSRTTTSTTTTTGTTTSSNNQTNQHAYAPISQTHLERSHPNHPIDIERSTTNSKLGLDLNHNNRHSDQSTIKAACPQLLHPAPHPSFQSNQLLNWNENSINNPSNTSYYTPSRFTRIIRCCHLKPLLFLAIVAFIGFQITTHHSSFSLGDHKSPDSFAQISRSTFGANNPTQNLSPTSEGVIEKTEREAQDTLEVIQETNKGEKPDSLKQKIGRLSAWLCAFLYLTSRIPQIMKNHSRKSVEGLSILLFVLAFLGNLTYVLSILSSPQILFNNPDDANKDHRKLDFLNEAIPYLIGSAGTLCFDLAIFIQSRLYRSHPTQPSPSSTLPDSPSITCSHLVNPSPPLLSPSTHPNQARLNPDPEIYAALPLLDSRSSTSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYE9</accession>
    <name>A0A5B0QYE9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYE9"/>
    <dbReference type="OrthoDB" id="10608728at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="303" mass="33545" checksum="7DCD9E503197ADF9" modified="2019-11-13" version="1">MTRDQTPDQDIQVVHDSVSTPSCQSSSFMIRQSARKRNPLVNPGFVPTQADSRKALQANVRVTSLNGHTSSPEAAPNTSSTPTATTQEDPKSQEPATSSSFNSASLYDLEQNSDDENAQAAGRSKKSKEDRKLAPRKDGTQSVLLYFKQVEHSLNYACVWCSKIVKASSSSYYNLKVHRDGSNIKGTIRQACPNRLKAIASGSILPKTASAISQESLESKKPINNTLASYVIKGRFDNNTFNKLWYSGLSAIVSHGPDLKTEPYVLPWTMWTQQPNFTLERGLPKQHTNSTWLNSVLFWKISM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZ24</accession>
    <name>A0A5B0QZ24_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="5 17">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">HEM2_2</name>
        <name evidence="19" type="ORF">PGT21_029729</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 17">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the ALAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="5 17"/>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZ24"/>
    <dbReference type="OrthoDB" id="2495917at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000048">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1118014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="337" mass="36318" checksum="C3835676E732860D" modified="2019-11-13" version="1">MTTEPSSMATSLSHVLQGGYAHPLTRSLQAERSLTKDMLMFPLFISDDPDAKQPINSFPNQYHLGINVLEDFLRPLIDKGLRSVILFGVPMKCKKDARGTPADDETGPTISAIRLLKLKFPSLFVAADVCLCEYTEHGHCGFAPGKPGGEIDTPVSVARIAAVALSYARAGADCVAPSDMMDGRVGAIKQALIDARLANRCCLMSYSAKFASSLYGPFRAAACSAPSFGDRKGYQLPPNARGLARRAIVRDISEGADIIMVKPAQMYLDVLSEARSLAPSHPLACYQVSGEYAALLAGADAGVYELKPMVFESLDCMLRAGATLILTYFTPMLLDWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R389</accession>
    <name>A0A5B0R389_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R389"/>
    <dbReference type="OrthoDB" id="10289914at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023137084" description="Secreted protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13697" checksum="DF1D14ED96358789" modified="2019-11-13" version="1">MKLSITNAFGIPMVLLLFQLLASQVACHGCDKAGQDRNGVPRQYPLCAGNVKRTNEHFSGGGSQTNTLVEFVAPPWNVDHYDCSQTDNYKTEACCKTTGLKFILSLEYMDKDCSRPDGHPLNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RCG9</accession>
    <name>A0A5B0RCG9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fungal lipase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027523</name>
        <name evidence="4" type="ORF">PGTUg99_011788</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000208">
        <property type="protein sequence ID" value="KAA1123446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RCG9"/>
    <dbReference type="OrthoDB" id="426718at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF25">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366587" description="Fungal lipase-like domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1123446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="37392" checksum="742CF5ED5047ADEA" modified="2019-11-13" version="1">MSPNLHLVILFVFFVSITLSRSHPQGSQPRTTSPLPVAPSTPRPATVRGSTPSRTTGTTKTQASHGAIRGTGLIPPQGDHPEWSYRNGEYIRDQQDHFRFHCYLAMSPYGDYSHKCPIAFPGGFQVLQHFSGGYIARVEEMEKVVIVFKGFATYQDVDLTPASVSSFVSDCADCMVAAGVRNQYLRLKAETNDFAVAKNAVATSTNNGGKQMLFSVTGLGWGGALAALCALELGSQNWVHYSHNSGTPRFMNDAAVLRYDNLFQFLAGQSVVSNNDYAVQYVPKGVYSHIGQKVRIHGEKSQWYVNCFGNNENSTCLGDGSSSADHTFYFTPIGQCGSADKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RDI5</accession>
    <name>A0A5B0RDI5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022651</name>
        <name evidence="3" type="ORF">PGTUg99_011450</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000216">
        <property type="protein sequence ID" value="KAA1122814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RDI5"/>
    <dbReference type="OrthoDB" id="2514099at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="112" mass="13102" checksum="AFB1747EFE490C2B" modified="2019-11-13" version="1">MQLLVIENESELEIKTHVEDLVTLEDQLSEAQQDYLEMQQARRRNLTTSQIMRLEQAPDTIAFLQEEINQVINKLDPETNALMKLVVSKSKLYEAKVVVMELQRRWDQRSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1F0</accession>
    <name>A0A5B0S1F0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP synthase subunit G atp20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_015329</name>
        <name evidence="12" type="ORF">PGTUg99_032554</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase g subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1F0"/>
    <dbReference type="OrthoDB" id="437at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006808">
        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1080632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1131592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18237" checksum="F066D79AA099BCD0" modified="2019-11-13" version="1">MSSLNQIRQRLLTIHRANTPSSCRHASTNSNPSPKINELTQKASDLANQYGSKLPELSQKIGKRFDGLLGSYSQPVSQNYQFTKEVLKQVYIKENLAPPKLEQIKSSYHEILRNVSSLNFWKQSLESGDWKRLAVYGVEAVGLFSIGEMIGRRHIVGYKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S595</accession>
    <name>A0A5B0S595_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Secretory subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SEC63_1</name>
        <name evidence="12" type="synonym">SEC63_2</name>
        <name evidence="12" type="ORF">PGT21_025119</name>
        <name evidence="13" type="ORF">PGTUg99_008505</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000078">
        <property type="protein sequence ID" value="KAA1132575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S595"/>
    <dbReference type="OrthoDB" id="1734229at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000039">
        <property type="entry name" value="Protein translocation complex component (Npl1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000281">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.4, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075">
        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075:SF0">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="11">
        <location>
            <begin position="97"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="470"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="606"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="607"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="645"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1070824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1132575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="669" mass="75374" checksum="19E23CA156B4A9DA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S8C2</accession>
    <name>A0A5B0S8C2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RING-box protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RBX1_3</name>
        <name evidence="6" type="synonym">RBX1_2</name>
        <name evidence="6" type="ORF">PGT21_010186</name>
        <name evidence="7" type="ORF">PGTUg99_026764</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S8C2"/>
    <dbReference type="OrthoDB" id="8962942at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1097522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1134108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="81" mass="9229" checksum="040BF0ADAC09EA1C" modified="2019-11-13" version="1">MADSMDVDQNSVTKPDPEPTSNEQKRFVVKKWNAVALWAWDIIVDNCAICRNHIMDLCKEHIACGHFSILFRSNILIGLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SAE3</accession>
    <name>A0A5B0SAE3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026601</name>
        <name evidence="3" type="ORF">PGTUg99_003619</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SAE3"/>
    <dbReference type="OrthoDB" id="2503740at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="269" mass="29493" checksum="FC1D89DAFE8463E0" modified="2019-11-13" version="1">MLIPLNLPRSRSSTGSSCGFHEIPPANLNLLRPSDAESSKQQHAGRRLSKMRNMISSTLTRSHSQYPLPPSNYTQTLPTSRPKVRRTISRSMIAPLQHLFGVDHHESELEEAERYENQPWSLTLSKASTSKFSRRGPSTVLYQPTLSDDDEEWDFRCQGMAPVIPARPSEDALSPSSAYSHSFLGSPGYLGDPGFFNAASSASSCCSSPGPCHVPLRSFSADNGVKKQKISVDSISGPLNLIPSRSLSPLLAPPLPQRKRNQLGLEVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJC9</accession>
    <name>A0A5B0LJC9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000943</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000200">
        <property type="protein sequence ID" value="KAA1063990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJC9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1063990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="127" mass="14223" checksum="17F64C28129CB11F" modified="2019-11-13" version="1">MLWGIVVNAPNSEPITSLLDDHRYLTRNKPFKPSLVSTKQLNQSLDNLSHRTTEDVKTRPNFSTHLIVNSSINFNQALFWSARASANVTARIQDIGRPSAESSMNLIVLMGLANKLPQAKALFKTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKK8</accession>
    <name>A0A5B0LKK8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_002356</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKK8"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969:SF69">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="75"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="170"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="275"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1064420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="315" mass="35654" checksum="6F6DB3C837998BE7" modified="2019-11-13" version="1">MKFQKFISSILPIFSSSLITNLLPQAAALVPGSKSIATEEVFSEARDLPRDKELVEHSTSHDLSGSLKSEESSKCAICQKNLGREGVKELQCGHIFHQTGLDRWAIENPVCPLCREEDHSLVIQHQEHKMLQEAKERERQIEEQNRIQLQNERARQEEITRDISYQDGDCLICSTRAVPSNLDSPYCPGCGKPIHELCLNRWLHGSTTYICPACSTDYFQWHQEQLSDDIDLEAHEHDALTHLTDHEGQGQAHLLRDSSAFSSLVSSRKRMREYASENERVGLSLASSSHANKQRDRADPTREDHDRDGSDENAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLC6</accession>
    <name>A0A5B0LLC6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MED6_1</name>
        <name evidence="8" type="synonym">MED6</name>
        <name evidence="10" type="ORF">PGT21_024300</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLC6"/>
    <dbReference type="OrthoDB" id="344220at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.580">
        <property type="entry name" value="Mediator complex, subunit Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007018">
        <property type="entry name" value="Mediator_Med6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038566">
        <property type="entry name" value="Mediator_Med6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13104">
        <property type="entry name" value="MED-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04934">
        <property type="entry name" value="Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="166"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1065085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="265" mass="30136" checksum="7D975735D9DFB513" modified="2019-11-13" version="1">MTSTIAGLPIPDSDLCHVQWRSLAWILEHGPLTESNAIDYFALSPFYDRKSTNQVLRMQSMFSGQPKMDPASEQEALRRFIGIEYALVLSRPEPDREGGLFIIEKRERKGYDEYYPIASYYILKTSIYQAPSLYATLSARVLTSLSALQRLLELARQHKPTYDPRHSYAWKIKEKPEENTVDKQSTDQPTTESTTEVEPTTQESDRMDIDMTAPEKPDGPAKTTLKKKNHDTPELSNPILFRALQNTITNFPAFKPPPAVDAVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLD9</accession>
    <name>A0A5B0LLD9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLD9"/>
    <dbReference type="OrthoDB" id="2506931at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="11378" checksum="B4825F398AF7519E" modified="2019-11-13" version="1">MGLTPRPKPRVPALFPDLAAPLPQPVKGDRSFRAQQHSIRLWFEQVAARQHREEDKATALLVSMAFAGGPIQQMRTYLTRGDLPGDPMADSAWAYMFSGQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMT6</accession>
    <name>A0A5B0LMT6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026236</name>
        <name evidence="3" type="ORF">PGTUg99_022593</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMT6"/>
    <dbReference type="OrthoDB" id="2500247at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137005" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="12626" checksum="B7F24ADBED74A634" modified="2019-11-13" version="1">MIKTCLPSLVVVIVGLLSVARAVDVTGAYSYYPVNATVLHVDANEGSFNCPSSCTSYIKATGCTGMSKKQTDEICSRSFGATSAAAKLCTNIVKDKIKQFSCTGFDPASKFSCSGCTKIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNB2</accession>
    <name>A0A5B0LNB2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007728</name>
        <name evidence="2" type="ORF">PGTUg99_005436</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000518">
        <property type="protein sequence ID" value="KAA1063927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNB2"/>
    <dbReference type="OrthoDB" id="10346601at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036136997" evidence="1">
        <location>
            <begin position="22"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1063927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="72" mass="8029" checksum="7D07B2C61FECE087" modified="2019-11-13" version="1">MYPIPSVVFLILTTSLGLITAVEEQRDARHPFKCTGIHPVGYCGQKQGYKVYSSKRDLVDHLIEVPPSDTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRB0</accession>
    <name>A0A5B0LRB0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRB0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="13196" checksum="78EA27B3EE159F58" modified="2019-11-13" version="1">MHTPPESDNKSLVKASFHVFMEQEPQTFPDMQMTQPPKYPLKVEDHVPRERNDRYYFELAVSRKENGLDTSSTSRKDKLECKMKSLEKEEREASFCSSDIAGFQRSRFSKQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRF3</accession>
    <name>A0A5B0LRF3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRF3"/>
    <dbReference type="OrthoDB" id="2512529at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="388" mass="41531" checksum="AA62656DE5C25873" modified="2019-11-13" version="1">MGQGCSTMGELNSPMVSMGIPLDQVAASCWWTMEDQRQPTQLGRLATHDSSATTTDSPCSLLSLAIQTAFGLGHSRRTIAALGRLILPDCSRAWQAAQFPDWARSRVWLEYFRQSSTSVHQRKSPDSASEYRGSEESYSPGPNTPGPRTRATTQSSNRASQTGANRATVHRSHGTSDQSPSPAPDTPGPRTRVTTQSTNREPQTGPVRPTVRRTHGAGGRLPAGPQRGDSTRAAPAPGRGASRPRPANLPGVPSRARATALSTRQATAVQPTGNEADPQGAWPPATVHLTVDDEPINDEFVSEVDAIFQLGGSYADLGEQLAYVPLPRQYVVGIYGLLSVRQAIERLRHDILHAPRGPGAASAEVSMVSQAQNFHYVAVFSVSLTFPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LTW5</accession>
    <name>A0A5B0LTW5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">p24 complex component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">EMP24_2</name>
        <name evidence="11" type="synonym">EMP24_1</name>
        <name evidence="10" type="ORF">PGT21_018172</name>
        <name evidence="11" type="ORF">PGTUg99_012591</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LTW5"/>
    <dbReference type="OrthoDB" id="62956at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="47"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1067825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1093426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="213" mass="24404" checksum="8BA94021D16CF58C" modified="2019-11-13" version="1">MHLQNQQNLRSSWAKLIKDSRTALFLWSLLLSASVLTHTIDIPSGHVTCFFEDLHVDDKMTITFQVAGGGHLDIDFWLNDPQGNHLWNVIKRDTGTFTFTATQGGKHEYCFSNAMSTVSSKTVSFNVHGVMYVEDDAKMLPIELEIKHLASALEAVKEEQQYIVVREKLHRNTAESTNDRVKWWSLVQTAMVLGVTAWQVFYLKRFFEVKRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVG7</accession>
    <name>A0A5B0LVG7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010033</name>
        <name evidence="4" type="ORF">PGTUg99_003282</name>
        <name evidence="3" type="ORF">PGTUg99_015938</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000377">
        <property type="protein sequence ID" value="KAA1092182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000191">
        <property type="protein sequence ID" value="KAA1124996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVG7"/>
    <dbReference type="OrthoDB" id="2509082at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1124996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="273" mass="31292" checksum="639D013ECD65F49E" modified="2019-11-13" version="1">MLLHPGSLAIYGTLSILIAEIPSQCSGASLSKRSPNLEGIYSMFKGEEGSMKSIHQILGEKMGADPNNPEKVYQSTVKIKAIRNVAKEKLGVAPKVTDVGAKYLQKIVEGDHEGAKRTLEKFPRAGLYDAFRGISQLNWVELWKITTEPGSKNFIREVESEAEIIQEVDTAAYLNPGMTNADMEDFRRFIIRKKRTEPEADQEVSHDLPGDEKLTPQEKIKKQRERKVNPLYDEWKKSKKPVRFYLLRWWKKFVRGSKAIYRRFMSLFRKKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LY11</accession>
    <name>A0A5B0LY11_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013150</name>
        <name evidence="2" type="ORF">PGTUg99_037140</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LY11"/>
    <dbReference type="OrthoDB" id="10496512at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="179" mass="19680" checksum="D89CFDE3C90A1202" modified="2019-11-13" version="1">MQFILTNPGRYSRRNGHLHVLIEEKAAFIELELLAGSRPEIASLPGNSEQAAMEIVTPDHSAFEETPTLPSVKRPPTTSSSTTQFNHYQPHFTSNHPNPYFPPNTLSKTTSKMPQWGNGSHMCILTKDFEYPVCVPEHNGKPWGNGSHICILTHNGKGWGNGSHICVLNRGAQVAADVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZ63</accession>
    <name>A0A5B0LZ63_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZ63"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="8860" checksum="2A067ECA8E482E82" modified="2019-11-13" version="1">MSRPQRISSFQSQVCFMIAKIFPITVRKLEHSLSAINQQISCKFNPPPQQFCRPFGLLANLPSSVLKCVADNLLQSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZD3</accession>
    <name>A0A5B0LZD3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Microtubule-associated protein, microtubule dynamics during spindle orientation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">STU2_5</name>
        <name evidence="19" type="ORF">PGT21_034616</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CLASP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the TOG/XMAP215 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="205662at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990498">
        <property type="term" value="C:mitotic spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099070">
        <property type="term" value="C:static microtubule bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990571">
        <property type="term" value="P:meiotic centromere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000608">
        <property type="entry name" value="Centrosomal protein 224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000052">
        <property type="entry name" value="Cytoskeleton associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000019">
        <property type="entry name" value="Cytoskeleton-associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000050">
        <property type="entry name" value="Cytoskeleton-associated protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000068">
        <property type="entry name" value="cytoskeleton-associated protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21040">
        <property type="entry name" value="CEP104-like_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="14" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="18">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="18">
        <location>
            <begin position="297"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="18">
        <location>
            <begin position="622"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="18">
        <location>
            <begin position="889"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="16">
        <location>
            <begin position="982"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="18">
        <location>
            <begin position="1232"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="237"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="531"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="847"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1120"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1484"/>
            <end position="1615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1902"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="2039"/>
            <end position="2069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="531"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="554"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="588"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="864"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1120"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1187"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1484"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1535"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1557"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1573"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1905"/>
            <end position="1926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1927"/>
            <end position="1940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1949"/>
            <end position="1959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1069865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="2117" mass="228260" checksum="CAC653C9FECC268F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M4D3</accession>
    <name>A0A5B0M4D3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M4D3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF22">
        <property type="entry name" value="TRANSPOSON TY3-I GAG POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="440"/>
            <end position="465"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="75606" checksum="C985FC5AB5424FA8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M540</accession>
    <name>A0A5B0M540_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013399</name>
        <name evidence="1" type="ORF">PGTUg99_020763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M540"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="107" mass="12002" checksum="6A732AB2796B3ED8" modified="2019-11-13" version="1">MISIFRSNQIRFFQKRERAGKKTLQSTCHSNHLIAIFPGIFVPTPCPSCFLGIPILSKSSNCGPLGNHWAILIALTAEATSLKKHDPRKFQSYTTRGSTSQISELYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6E5</accession>
    <name>A0A5B0M6E5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6E5"/>
    <dbReference type="OrthoDB" id="10562514at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="179" mass="19680" checksum="86251AC9C608FDBE" modified="2019-11-13" version="1">MSSAVTPSDSACHPSDACRSRYSSGRSWTGLSPPLPPLVRSKPRFHPSVPPLLVAPPQYTLNHRSAVVREPWCPPVPGFPAASFSEPCAFGLLFPALPRLGKLDFPLMYRHFPQLACVASAPHAFRISRKHSACPQTTWSRRALATTASTQTSFIWVLSTLLTLRLMCLFAHSTDEELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8B7</accession>
    <name>A0A5B0M8B7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8B7"/>
    <dbReference type="OrthoDB" id="2017782at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="750"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="874" mass="99989" checksum="CFE90DB70C753167" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCK6</accession>
    <name>A0A5B0MCK6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras GTPase activating protein ira2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_025330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903479">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21206">
        <property type="entry name" value="CH_IQGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149:SF14">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN WITH IQ MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="345"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="2">
        <location>
            <begin position="1130"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1073778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1509" mass="170000" checksum="58A41966F1F64CEE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDS3</accession>
    <name>A0A5B0MDS3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDS3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="47"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="347" mass="38612" checksum="469526999FFC0605" modified="2019-11-13" version="1">MRLGPRPTHTTQGWVVWVGLGPLYKVLTLNNDRSRFNLDRSLFKDPGLGPTQLHNTPGAGSGPAPRPGLYLSSDSRARSHPLLSYPQPPSHRGDPKTDKRPATEPEEHPDSRHRHPRNPFEAGDPRPPDTPLIRSDPIRSGTQKLIHQDMSDKTSNALGGGYNHHRPNSAATNNQESQHNSGLEIVRRDLNTRTDGRSPSTLERLKHPSSPIERPTTRPTNALSIAIKTRRSELNDLQALRSKSLCRMSDIPRGRVRVRAKSVASRSSSQSYNPQEEDGSQETPSTQPCTQPPSASEQAPTDQEELLRAQRVAQSSASSTYTSYREPELSDQRDKHGRRMIAYPCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEM2</accession>
    <name>A0A5B0MEM2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009597</name>
        <name evidence="4" type="ORF">PGTUg99_033061</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000271">
        <property type="protein sequence ID" value="KAA1116031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEM2"/>
    <dbReference type="OrthoDB" id="10330887at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137200" evidence="2">
        <location>
            <begin position="35"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1116031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="155" mass="16307" checksum="AA9A0F0B4277A0D1" modified="2019-11-13" version="1">MIQIVSGGMFCSKNLWLLAAIVLTISLIMQSAQCEEDQANAPIAVKGEVGEATTRITNHASPSAAGIVRRSPLPRKKKKTQAAAASQLKTAQKNKGQKLAVAQTNNAGTGTGVNTGGGVGNCKAPKTFFNPKTKRCECPNGKKESRNQQGQIICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGH5</accession>
    <name>A0A5B0MGH5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA protein C-terminal winged helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028444</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGH5"/>
    <dbReference type="OrthoDB" id="511599at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056808">
        <property type="entry name" value="HTH_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36168">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36168:SF1">
        <property type="entry name" value="ORC1-LIKE AAA ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24913">
        <property type="entry name" value="WHD_AAA_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA protein C-terminal winged helix" evidence="2">
        <location>
            <begin position="424"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="574" mass="64165" checksum="A1DA1158F44B37CD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MK36</accession>
    <name>A0A5B0MK36_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004472</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MK36"/>
    <dbReference type="OrthoDB" id="2501916at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="252"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="322" mass="37054" checksum="E675152367455729" modified="2019-11-13" version="1">MTCHSISTTSLLIQAYNHFVHFVQHAKYQKEKKQEGKNAQDATNKRISKNRERLRDAQKEFAILNKFPQRYVDILSHIGAHSDDKHDERKNVYKIKTLVYCSKNASKFIRALDIAMKNAAEQDPSSNQKRRVHQLPRQPVISTFTAAPKRLPIDFYDPSWYHKLVPAQQKTIPDTHNVAFLPDASQSLLPKKQRHPDEKLTDRSFTRKYWDILVEPYGLLGEDSSDDSDDEEASPEGMAKSTGNNSEGEGHDLDDESPDESPDEYFEEGEAGDLYDDFVEDDEEGDDEEEYNANEGDEDDDEDQDEQYGDSFENTEDVHMQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKK9</accession>
    <name>A0A5B0MKK9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">orotate phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">URA5_2</name>
        <name evidence="11" type="ORF">PGT21_016237</name>
        <name evidence="10" type="ORF">PGTUg99_010990</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="5"/>
    <dbReference type="EMBL" id="VDEP01000451">
        <property type="protein sequence ID" value="KAA1077251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKK9"/>
    <dbReference type="OrthoDB" id="269405at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046132">
        <property type="term" value="P:pyrimidine ribonucleoside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000052">
        <property type="entry name" value="Orotate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683:SF1">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="9">
        <location>
            <begin position="75"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1077251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1092857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="247" mass="27367" checksum="BCC5F2C31E957FA7" modified="2019-11-13" version="1">MGTEDIQPSLAPVDHSQLVPTTDDDYSTRLIKSSISHGILKFGTFELKSGRSSPYFYNSGLFNTGFLLNLLGLSYASKIIELKDELEFDVIFGPAYKGIPIASITAAELNRLDPLKYHSLGFSFDRKEAKTHGEGGSIIGSPLEGKRVLIVDDVITAGTAIRQSIKTIEQHGGHFVGVLVNLDRQEKSNDSELSAIEIVEAEFKTKVHSIIKLNQIIDLLKSIDGYQNELLQMENYRKQWGIQPAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MM97</accession>
    <name>A0A5B0MM97_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MM97"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023150290" evidence="1">
        <location>
            <begin position="22"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="8512" checksum="CACDD53518737D0E" modified="2019-11-13" version="1">MGILQIFTVILVIMIAHHFNGSPAPGMGASWSQCMATANNIPYKGRPATNVEGDLSPCQRLLRYTMTMSCPEIETPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMH0</accession>
    <name>A0A5B0MMH0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_050236</name>
        <name evidence="2" type="ORF">PGTUg99_050021</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000463">
        <property type="protein sequence ID" value="KAA1077060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMH0"/>
    <dbReference type="OrthoDB" id="3344688at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="176" mass="19794" checksum="9E1EF269828FAE0A" modified="2019-11-13" version="1">MDIRLHFVRKLIQLALIKLHYVKTTENIANFLTKALGRATIRKSLAAIGILQVKEAASNLTPRSTPGCWKPNSRDRDSNPKRARTQQKVYTAVIDHKQSTNVIEPAMEPNDSTNPNRSSLLDRLSSPIAPALFSKNLVSEPIDKSLVADNYNNQTTSLYPEENKETSAHRITQQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMR1</accession>
    <name>A0A5B0MMR1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_001729</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
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        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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    </dbReference>
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        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="540" mass="60954" checksum="C5108A72F5C2B004" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNB3</accession>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
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                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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                <person name="Schwessinger B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
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                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Schwessinger B."/>
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                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21369" checksum="289F87CF1B750AB2" modified="2019-11-13" version="1">MMLLSISLLVSMWIIQSVDSRSLAHIITRDGETHRFTKRAKLIPNQTYVQIQISDGPTGKCGKKAEDKFLSVFGLTQAQLIKQNPFQGPIDPIDLKTCNQMTRLAVDAEQGFNNAIGNQSTKFGIGKQLDNGKTCNKVLKLTGTVLGLQMEIKLGKADATRLRKLANEINKLQKNIDADARAPNQKQRSFLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQ17</accession>
    <name>A0A5B0MQ17_PUCGR</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    </protein>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2506685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="326"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="469"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
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            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="478"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1078208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="698" mass="80018" checksum="58B960A911A2612F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQT3</accession>
    <name>A0A5B0MQT3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQT3"/>
    <dbReference type="OrthoDB" id="2501290at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="713"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="754"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="796"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="856" mass="93403" checksum="7579A3FDA0DD6C18" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSV6</accession>
    <name>A0A5B0MSV6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSV6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="66" mass="7235" checksum="D44C4B1B38DF178A" modified="2019-11-13" version="1">MVPAKQALNRGASRPDSNLPVREAIVTLDIIPIPTPSDDDLFRFESALHCQERLTSLAALFPCKMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTD0</accession>
    <name>A0A5B0MTD0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTD0"/>
    <dbReference type="OrthoDB" id="2505311at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="407"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
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            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="202"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="667" mass="74696" checksum="E7571E403AE5F43F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MW02</accession>
    <name>A0A5B0MW02_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MW02"/>
    <dbReference type="OrthoDB" id="2414509at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="412" mass="45528" checksum="018F1201126875C8" modified="2019-11-13" version="1">MSQEISEVRPDLPDLTIPAPLNQAADSSTTPKPKRNRRTNAEMEIYRASQGIEKSTSKKTNNEKEFKYWVPTARRLAALKKNSNAHRSAAKPATSTSRTADSNPQFIATDYQLICSYLEDPQHYTQIYGNGTRTSVGPQSLTKGAAYDVFAIYMNDQTNKRLHLDGKLLRQRIEAYKKRFTSAKHWADTTGAGIDICDNGITIKAKLESMCPCYERMDQIFGGKPNVTPAAQYESQGGMELYDQPMDDDQLRSTELICSNREVNENQGTLSAQDQPEDQSHIAAAGEETTMGGNAELPEGELLVAFRCTNQVLTPHSRSIAEPNTRSTPALQAETLLSVQSQPLPHPLSIQTGTNQPRRTMASILGNLQRSVALDEAEDAADEVEDPPMEPGPSQGRFPNQNADNQGSQHRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYU0</accession>
    <name>A0A5B0MYU0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TUB2_5</name>
        <name evidence="18" type="synonym">TUB2_2</name>
        <name evidence="16" type="synonym">TUB2_4</name>
        <name evidence="16" type="ORF">PGT21_026546</name>
        <name evidence="17" type="ORF">PGT21_027769</name>
        <name evidence="18" type="ORF">PGTUg99_021281</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="18 20">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYU0"/>
    <dbReference type="SMR" id="A0A5B0MYU0"/>
    <dbReference type="OMA" id="WVPRSVN"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097435">
        <property type="term" value="P:supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000006">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="46"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1074992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1081020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1131176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="448" mass="50152" checksum="B674EA56E7451C29" modified="2019-11-13" version="1">MREIVHLQTGQCGNQIGAKFWEVVSDEHGIATDGQYKGTTDLQLERISVYYNEVATNKYVPRAVLIDLEPGTMDSVRSGAFGSLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREEFPDRMMATFSVVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKLATPTYGDLNHLVSIVMSGITTCLRFPGQLNSDLRKLAVNMVPFPRLHFFMVGFAPLTARGSQQYRAITVPELTSQMFDAKNMMAASDPRHGRYLTVAAYFRGKVSMKEVEENMLSVQSKNSNYFVEWIPNNVQTAHCDIAPRAHKMSVTFIGNSTAIQDLFKRVADQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMQDLVAEYQQYQEAHLDDDEEVEEAYEDEAPPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZW0</accession>
    <name>A0A5B0MZW0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034612</name>
        <name evidence="2" type="ORF">PGTUg99_012150</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000379">
        <property type="protein sequence ID" value="KAA1092004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZW0"/>
    <dbReference type="OrthoDB" id="2495105at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1081359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="156" mass="16943" checksum="B09AEEA29ED83DCF" modified="2019-11-13" version="1">MFAVKHTSRVMIHILFIGMITTLCITVTNAHTCSFSFQTEEGGWSPPSHKASCDDGSRTYLCSLSGCKATSPDGPTDLKKSVFTGCSKDYDSPTGTLSVYVLSYNWFDGGKLSVTGKNYPSNEGDLEVYCPDSPENRKHMPFCDDSACDGGVPDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1A7</accession>
    <name>A0A5B0N1A7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021880</name>
        <name evidence="2" type="ORF">PGTUg99_026810</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1A7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1124090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="147" mass="16063" checksum="5E0E619D0B3BB6FF" modified="2019-11-13" version="1">MLVKCQVAPLSAISCNVLYILRWSINTVAVIDPRLAGHGKPTIALKSQMHIDSITEQEVINNKKMFHVFQNRDIPTGITATSQQHIYTGTQTLTDVAPDPNLRVRVCECACSIVVSTAHEYAEVSPTPIGDFVILNESGLNNIGYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1J4</accession>
    <name>A0A5B0N1J4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1J4"/>
    <dbReference type="OrthoDB" id="10434130at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="13583" checksum="8EE84ADF4101DEC2" modified="2019-11-13" version="1">MAVEVDRLLKKDANHNQAISIAKNHIRCFCHKLALILNAGLKAISVPRRDQIPTGPVLGFVPSLCAISEESSDSGQVDPLANPFDTDGFVDNSDSDCSDDEGPELREANVIEKSREVHPDQDLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2024-05-29" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N202</accession>
    <name>A0A5B0N202_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011580</name>
        <name evidence="2" type="ORF">PGTUg99_022063</name>
        <name evidence="3" type="ORF">PGTUg99_036714</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000439">
        <property type="protein sequence ID" value="KAA1082130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000337">
        <property type="protein sequence ID" value="KAA1102859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N202"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="64" mass="7140" checksum="064B4539C8B4A674" modified="2019-11-13" version="1">MKSSYLFKSFPSHERSLIGLLYPQDSHHLHLLVIQDLHGSSELIHTPNPLSICSVNSSNFVGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4Z3</accession>
    <name>A0A5B0N4Z3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4Z3"/>
    <dbReference type="OrthoDB" id="2367685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="27090" checksum="14CA653AF71C2E7A" modified="2019-11-13" version="1">MTGYSTQNYNEQPLPVGWIKEWDSNHSAWYFVDTRQNPPLITWHDPRHSAPPPQGPPPAAGGDRGLMGSLGSMISGKPPKNPNNAYSQNPQGPYNNQSAYQAPLQQYTPGGEASSYYNQPAPPANYHQAPPPPPNNGTKPGGGGGLGSLLSKIPGGAGGSAGNGGGGAGAMLSKIPGMLGGGGSSSGGGGGGGGTGSKTNPLLAGAGGAAAAALAGKLLGGGHKNSHHAGGHSGGHHGGGLMSMIPGQHHGHHHGHHHGHHHGHHHGHHHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6G3</accession>
    <name>A0A5B0N6G3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_101</name>
        <name evidence="2" type="ORF">PGT21_035957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6G3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="566" mass="65052" checksum="3469CE48B2A5F334" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBX4</accession>
    <name>A0A5B0NBX4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBX4"/>
    <dbReference type="OrthoDB" id="2367685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="71"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="241" mass="25784" checksum="B4373B2420F56B50" modified="2019-11-13" version="1">MGIFDKLVSTPKPGGAGGGGGPPSYTQANNTSTNPYPSTYNNQAGYHPYDANNGSSLGGPSAGAAGTDQRPLPPGWEKQWHDGYQRYFYVDTRAPNGPQSHWIHPIDQQQQQQQQSLPAGAEAGRLYNPPPPSNHPYYPSQPQPAGYQQPMYQHQPMPVGQPAPNPRRAGGGMNPLLAGAGGLAGGMLLGSMLEGHHHHHQDHNYMDGYEQGYENGYDNGFENGDEWGGGGDDWGGGGDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NC95</accession>
    <name>A0A5B0NC95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005808</name>
        <name evidence="3" type="ORF">PGTUg99_009250</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000116">
        <property type="protein sequence ID" value="KAA1129946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NC95"/>
    <dbReference type="OrthoDB" id="2495273at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137439" evidence="1">
        <location>
            <begin position="25"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="116" mass="12812" checksum="D01C6651E14DD105" modified="2019-11-13" version="1">MQSFNLFIVFAVLLINTQFYGANAFGCPNPGAPANRKFAYCTRTITAAEHKADPSLKDFTMYKEFLKLGDGYTCSKKVLKGKPAEFSYCCDVEGQVGQVLGSPESMWTNKCSQVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGD4</accession>
    <name>A0A5B0NGD4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005411</name>
        <name evidence="1" type="ORF">PGTUg99_024372</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGD4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1088401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="56" mass="5851" checksum="CB266FEEAC4BDE18" modified="2019-11-13" version="1">MARQTSQMFLALPIGKIDSAGKSLIPKFDSSKPKYQALAGAGASDGYCTVLDCKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIB2</accession>
    <name>A0A5B0NIB2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005478</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIB2"/>
    <dbReference type="OMA" id="ESHAQQW"/>
    <dbReference type="OrthoDB" id="2501309at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="390" mass="44604" checksum="ACA46CDF91743185" modified="2019-11-13" version="1">MFQPFESHAEQWIKRYEKAVEEYSQKTLAAESQSSQSNLERFIRSAASAKPPIILPSFAQESFQRAFSENYLEDDYGGASLDLDPNLGQSAQVPLGRSKLIPLPPAFLNGDTTFERQDPQTVHTKFENITSTFIGIGGHLYQLREVVDYWLPRDSPETLEVARVRWWPSQLIEQARLRTIHEMNSIQISFTENLHLVNNTICDLREGAFGGRLNTKSMACAREMFETLRDLRKLLLRIQRPASDRARSLSTTSIWFSTKCLPPLMEDLQEPYMAERFDQNDPNQAHLGELFSEHCVGLTATIKILSEAYEHLSETFKTVDGICKTMIEGIHKLMLDVHLGQTTGEFIASWANRWERVTFALLLRVLGLPAINLGLQRDFPTMEVEYFVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NK17</accession>
    <name>A0A5B0NK17_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NK17"/>
    <dbReference type="OrthoDB" id="2500956at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="286" mass="31262" checksum="8C6EA472BC53BDD8" modified="2019-11-13" version="1">MSLTITTPSSRAHLLLSPDTKLSNPENQSKLNRTNLNHNNNLLQETSSSKKRKSLIAGSGSPVIQPVSSLTRADELKGLDSKAFRLLPINASGTMSPTSEEYTSDVYNQAKVLPAFSTRDRSQSVFDSKTPYTNSMAFFKGGLSKAHKFIGRALEGFPDKESEEEVSDESHDEDKDDLNMLYTVMVPLEFPDEEDGETTSSSSSSSSSPFEPDFDKDSSKNHSEGIPGHCFKKQSLSCLSTNNRHLAPQNEPLTNGIDSTLPSQFTSNPKPPSVPSQQSDLNQSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKY8</accession>
    <name>A0A5B0NKY8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKY8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023129394" description="Secreted protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13890" checksum="0DD1B6C79B503ECE" modified="2019-11-13" version="1">MHCHTLIVLLLLFICTPSWAVNRWRRCGVCSLIYLDVAEKKMDHIRQLSQTGTTSTKTCGELKPEKSSCNYVYPVELFICNLCDAHAWVITTEDHCPRHRQPEEKYFDPKFPDDDEDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NME7</accession>
    <name>A0A5B0NME7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_005395</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PRA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NME7"/>
    <dbReference type="OMA" id="PWTVFFN"/>
    <dbReference type="OrthoDB" id="63113at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF0">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1089058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="168" mass="18470" checksum="5965D36CE9865516" modified="2019-11-13" version="1">MAVALAMIQNVPQYISSFREQRLSAIRPFSEFFDYQRISRPNDLNAATSRITYNTRHFSGNYALIVAALAVYSLLTNPLLLISIGFLVGGFGCIQRFGPDPNMPSEGQMVTQKSLYITLFVIGIPMLWIAAPIATAMWLVGSSAVTVLGHASFLEPSVESEYSSVQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNY5</accession>
    <name>A0A5B0NNY5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="3">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DCW1_7</name>
        <name evidence="9" type="synonym">DCW1_15</name>
        <name evidence="8" type="ORF">PGT21_019915</name>
        <name evidence="9" type="ORF">PGTUg99_019750</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000177">
        <property type="protein sequence ID" value="KAA1125561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNY5"/>
    <dbReference type="OrthoDB" id="9984024at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF36">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1090985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1125561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="201" mass="22147" checksum="0199841EEABA7DBF" modified="2019-11-13" version="1">MITKDPETLEKAKSILEWVISSGIANPKTGLLMDGLSVKNCTEFTTFQWSYNYGQWLGSLAWMHKATGDQKYLDMATPYFDYSQRTFAASNTSGIVSELCEPDESCNRDQKGFKAIYARNLAYLHGETNNSTMKQAIEKVIDTSVQAMAAHSCDQDWNCAGNWTTDTHPIQFVRAQHVSAALLVAAVGIHGDSGLDASTCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQQ8</accession>
    <name>A0A5B0NQQ8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Imidazole glycerol phosphate synthase hisHF</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">Glutaminase</fullName>
                <ecNumber evidence="12">3.5.1.2</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">Cyclase</fullName>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="17" type="primary">HIS7_1</name>
        <name evidence="16" type="synonym">HIS7_2</name>
        <name evidence="17" type="ORF">PGT21_035320</name>
        <name evidence="16" type="ORF">PGTUg99_036635</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="16 19">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The glutaminase domain produces the ammonia necessary for the cyclase domain to produce IGP and AICAR from PRFAR. The ammonia is channeled to the active site of the cyclase domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">In the C-terminal section; belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="12"/>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQQ8"/>
    <dbReference type="OrthoDB" id="10254903at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000094">
        <property type="entry name" value="Imidazole glycerol phosphate synthase hisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000039">
        <property type="entry name" value="Imidazole glycerol phosphate synthase hisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014640">
        <property type="entry name" value="IGPS_HisHF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036936">
        <property type="entry name" value="IGPS_HisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 12 14" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="15">
        <location>
            <begin position="4"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR036936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036936-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1076165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1091535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="576" mass="62222" checksum="0656523FD9AFC543" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTV4</accession>
    <name>A0A5B0NTV4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">SUMO ligase siz1, variant 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SIZ1_1</name>
        <name evidence="12" type="synonym">SIZ1_3</name>
        <name evidence="12" type="ORF">PGT21_011115</name>
        <name evidence="13" type="ORF">PGTUg99_008802</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000377">
        <property type="protein sequence ID" value="KAA1092152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTV4"/>
    <dbReference type="OrthoDB" id="28127at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.780">
        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023321">
        <property type="entry name" value="PINIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038654">
        <property type="entry name" value="PINIT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51466">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PINIT" evidence="11">
        <location>
            <begin position="192"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="10">
        <location>
            <begin position="397"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="92"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="498"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="548"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1067605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1092152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="607" mass="66776" checksum="D1932611BD451756" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NY06</accession>
    <name>A0A5B0NY06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_008617</name>
        <name evidence="3" type="ORF">PGTUg99_033920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NY06"/>
    <dbReference type="OrthoDB" id="2511119at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="417"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="489"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1094065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="545" mass="59665" checksum="6FFF04F21C95ACD1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYX9</accession>
    <name>A0A5B0NYX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010932</name>
        <name evidence="3" type="ORF">PGTUg99_028815</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NYX9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="178" mass="19507" checksum="7613427890B9A6B0" modified="2019-11-13" version="1">MIYDNNDLNALNSNPSPYNFEGSDDMFAEPTSDVAEVLPPAYRDVAPMSVILPRSHRLATATQPPTSDPHSMAPTSPVTPMSPPTTPRMRPSAAPFMPAPVVDPPGPQTASDAMFSGSFLVAQFYNFLIMLESVLAEFPPNHPDAAIHTLLRMDAALLQDWFYHHRSHLKPAAHFLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P323</accession>
    <name>A0A5B0P323_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DDE-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P323"/>
    <dbReference type="OrthoDB" id="2505055at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF73">
        <property type="entry name" value="PROTEIN PDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE-1" evidence="2">
        <location>
            <begin position="255"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="649"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="756" mass="85303" checksum="498A3B0ECBBA677C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5K9</accession>
    <name>A0A5B0P5K9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028329</name>
        <name evidence="3" type="ORF">PGTUg99_029554</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1108090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5K9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="165" mass="18597" checksum="276F3ED75814784E" modified="2019-11-13" version="1">MPEQTSPVLCPKTQGADEPALQHPPRKTSVLLECLFFIPYVHPTISPRKGATPPLVKQWTKITAKPPLMPWVTDIQSMTWEQFQTKAFKFLGSQCSDLIPAFEAVNKDKKIAWYASISGHPKYDSEKKFIILGPIGYLDFVTAAYSARAAKIVFKLIMKDPREDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P605</accession>
    <name>A0A5B0P605_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FMP27 WPPW motif-containing RBG unit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P605"/>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019449">
        <property type="entry name" value="FMP27_WPPW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF6">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01216">
        <property type="entry name" value="Fmp27_WPPW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMP27 WPPW motif-containing RBG unit" evidence="2">
        <location>
            <begin position="2"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="754" mass="85513" checksum="246BBEFA993591A7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P6G5</accession>
    <name>A0A5B0P6G5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P6G5"/>
    <dbReference type="OrthoDB" id="2507065at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="435" mass="46613" checksum="4CA95C1F50CDA0E6" modified="2019-11-13" version="1">MVYNNQNPRHSYNSIPAKPSSLPVGSVAAQSADQQPARKSHLARAEANWVAFPPLPEAALHPFSSAVQFVSTTLKPLTEPSIAYPSGPLPNHLSRANTLLSSSSSASSSSSSSALNHLHSASHLSHGNSNGALKRSRTTTSSTHPTSISRKRSKSDFGSTSYRPAGSSQQPPIHPNIRPLVPSRKTQDFGSSSSLAHLNAPRSTRQRSRTPDPHTPALLIRQKQRLSEPARQASGPRKSSNSSPYPQYMSPAVRSSSSLKSSASVSSIGTLYPSRSRAHSQLNPIVSGVQDEDCPVGPVRRTYLGGVGVYLNNPQDEEDPNSMDDEDDDSPGGSPDGLQKTKPQLTINVGLCKNPSESAKSSANWYNRLLLSPLSSAGSSVYSFIMNGSSPLVPSDHHPDHSLELEHSPAQEPLRPAPSDSITASSWKHSFRRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P852</accession>
    <name>A0A5B0P852_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mannosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SMP3_1</name>
        <name evidence="12" type="ORF">PGT21_028539</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 22 family. PIGZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P852"/>
    <dbReference type="OrthoDB" id="10066429at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF3">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 11 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1096784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="301" mass="34155" checksum="912AC05A38B3EF69" modified="2019-11-13" version="1">MQSRKRDTSWKLSYLVAICLRLTIVLTSTSFIHPDEHFQNTEIAIADVFNRSNRQTRTWEWDPLRISDRTIGGGPVRSIVPVWMTSHLGLHVLKLLHNIGMIVSTASLISGTLLLSIQPHQEARFLLPLVFPLTTLCSQSISKSRSSKFRRTFWFAHLMHSAITVVLFGYLHQGGLQSALEVLPPNTETLLSYKTFDIPSSLITSTQLRNVTNLRGATEETLAKTLCEIVSMDEERRIVLLAPRWALSPSLEHRFKLLFSSAIPHLDLDRLDEIFHAGRHRSGIGLYSIHQQNMKASELCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8P3</accession>
    <name>A0A5B0P8P3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Methionine adenosyltransferase sam2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SAM2_1</name>
        <name evidence="9" type="ORF">PGT21_024897</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8P3"/>
    <dbReference type="OrthoDB" id="5852090at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="8">
        <location>
            <begin position="34"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1097947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="112" mass="12541" checksum="C555C8A0A3621702" modified="2019-11-13" version="1">MNTKLAKERQKEGGINWLCPDSITGQKIIINYWSKVNQSGAYLARWIAKSIIAARLAQQILVQLYYAIRVVKPLSTHVDSYGTSKGFTNTKLVDIIRCNFDLRPGVVVDADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEB8</accession>
    <name>A0A5B0PEB8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033054</name>
        <name evidence="2" type="ORF">PGTUg99_020355</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1099030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEB8"/>
    <dbReference type="OrthoDB" id="2519059at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="457" mass="49954" checksum="58DA3CD67E05F2AD" modified="2019-11-13" version="1">MQRGHHLRPSYQTSDRYEARIRHLEDVVCLLLARKDPHQLPFISTAPLNNSFRYCKQKGLVPLLSKAATESLSTDLQSIKQEGTIPLIKPGYYAFSYRSLFNCSPPVTQPPPPQPPAETTSRERQFKTTKGHEIMSAGIRRPRTISQVYQGRAHSEITDEQCPPVPALDRASSDQTQELRPSSSPAVPHCLPQSEVVKDSAPSPLILLKTPPLRSGSLDCFLSSDCPMSDDINRGLTKLEIQPSTGDPQSSCSTMTSSPPSENLDLKADDGLALFLSKDNLTSATNHRTDTPAHSPVNPIIIVADDSLPTSFLANPAATSATDNTSNLTPPLVSTSPPDPTATTPLLTTIPASTLQPEPAMPNCSSDSFDIAAIGSITVMEDSTAFKDPQLWAPSLSQAALENYKDIDDYLKTLEVDETEMKKKKKKKKKKKTPDPTSIPDNPVLFYAPFHPSLPYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFC1</accession>
    <name>A0A5B0PFC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFC1"/>
    <dbReference type="OrthoDB" id="2500993at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="469" mass="53184" checksum="0A9B27A55FDB736E" modified="2019-11-13" version="1">MNFNDPLFGDDGDPTASYCPEEEDESPAHSTPLPSSQHSPVSQKSPKRVRQTSEESEDEHDIVCDTNAQSDFSRLPPSTSMDKVCSALQSLYHLDDEHDQIARKASKCPTPERHANVMYGLLATQQHQSSIESSVPFGDTFKVRTNARMSFLIPTLEAYSNNPNKSGALPKSLYYISLPDEWRDDNLPPKQLKGESAALKLYTQVVGELLKHQRSHLRVLILTNILETKRIAITGPVPNRYALVTLIYSDLPPENRKWTEEQIKNRVESNWLMRIRMAYLRLVLVYFYTHPSSTGTQWGVIDQRLSILRKSTSEFQTMHATLVLKKDKELFSHGKQFHTIPKDQFSIPSVDDVNTALAQKDSTREMQALDAPDFPNLTKSLRAVDPMACRRDLRAMCHPRDMRIRRLVGHRFKTDLAHPFLNHFRPGLDGCDGLALAHPSISSSTFCSKWLLFKAAIAVLTDPDYAVWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLW5</accession>
    <name>A0A5B0PLW5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLW5"/>
    <dbReference type="OrthoDB" id="10313496at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022735257" evidence="2">
        <location>
            <begin position="20"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="126" mass="13718" checksum="6FAC636948C466BD" modified="2019-11-13" version="1">MAFQKILLVALLIASVAFGRSLVNLNRGLDIALHKRSLPAVDDFHHRSPQRRDSIPTQGKEGAEEQKEQANQHGDLKTGALKTSENVVAGSQDPNNVGSKHNNQYGDNKVHSVTFLPTPGHHEDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNE1</accession>
    <name>A0A5B0PNE1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNE1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="12181" checksum="F878832B766F6BD0" modified="2019-11-13" version="1">MYPDSMISSQSTALYAAVLPVGSLSRMILIGWTLLAIGDLLVIMCSLFEQGNHLNAAPVVLGGAHQNIDLSMCRDRWTSFNYPHPPSSRISAPSFTMQSIRNKIQPELMTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTN6</accession>
    <name>A0A5B0PTN6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTN6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="148" mass="16996" checksum="EEB07C33B2D67298" modified="2019-11-13" version="1">MRYAGSDLISLPWSACVLHRNSTNSNFNIQLKLQFRAQPTRTSIPYSTNSNFNSVLNQLELQFRTQPTRTSIPYSTNSNFNSVLKILQASRFRTQDSSIFQDHLSQLSSPLDLRLISSGSWFVVRCDSWSSWNLQGAFKRLIVRRSCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTQ3</accession>
    <name>A0A5B0PTQ3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTQ3"/>
    <dbReference type="OrthoDB" id="2107880at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20180">
        <property type="entry name" value="UQCC2_CBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="132" mass="15327" checksum="F31F4C9F2FA00824" modified="2019-11-13" version="1">MASSQRLMKIQSELKQIQRAWPLDPLRINQPELQISSSISKAIERVFSNNYKNDQSLSSQELLEQKTLEGAEKMLSSLENLSNGSIARQYPFPKSMRNPASFPGHYEELNEGVQRAIRGESLPWYRRWIRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PX18</accession>
    <name>A0A5B0PX18_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MRT4_2</name>
        <name evidence="8" type="synonym">MRT4_3</name>
        <name evidence="9" type="ORF">PGT21_007621</name>
        <name evidence="8" type="ORF">PGTUg99_029055</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">Associates with the pre-60S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PX18"/>
    <dbReference type="OrthoDB" id="10262308at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000003">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000038">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="7">
        <location>
            <begin position="125"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1088454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1105446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="235" mass="26409" checksum="6B1542A42780369C" modified="2019-11-13" version="1">MPKSKRQTIVHLSKTSKKTKEVKAKLIEQIKEASENFQFVWLFTVDHVRTAYLQDIRSSWKPSRIFMGRNAVMRLGLGSKEEDEHMPGLGAIGKLLEGDTGLLFTNEPPKVVVEWFDDYVKADYARKGNLATETVELPAGPVMIKEINDEPSVAPGALEPHLRALGLPTTLQSRIPTLSSPHVVCKEGEKLDTNQAGLLKTLGYQMAQFKIVLSHVWIKDRSTTFSIDQIRDQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXU1</accession>
    <name>A0A5B0PXU1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXU1"/>
    <dbReference type="OrthoDB" id="21266at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070692">
        <property type="term" value="C:CTDK-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032786">
        <property type="term" value="P:positive regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042326">
        <property type="entry name" value="Ctk3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024637">
        <property type="entry name" value="Ctk3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024638">
        <property type="entry name" value="Ctk3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28291">
        <property type="entry name" value="CTD KINASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28291:SF1">
        <property type="entry name" value="CTD KINASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12243">
        <property type="entry name" value="CTK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12350">
        <property type="entry name" value="CTK3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="1">
        <location>
            <begin position="2"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="330" mass="37279" checksum="7C2D7A264FA08C2F" modified="2019-11-13" version="1">MDGFQVRLQLVSILRKLSSSQNSIQTTIRFLLKHKDKYGEDLWDCLIEEAEKVNLNARINILYLLDGLLFTSSTALLTSHQDYQYQKGRTHAIGSGPSITPQNPSGGFNYGYLIKKDIIRFVEIVVPTSFNKKGLLNLMSTLQVIKNWKSQVSLIDQFLNIELLEQIDLLLNNRKQVLGKADVDHQGSDNELEAFSKKEILDRIDDDRERHKRLRERIWVLPIPSIATLSPKPTANAHHPATTYTYHRSATLLHTSLLSAGTGADRPVGPHIESDLTISLECDQLLQANLEDTGPDGLEELDRIAIKFDNSRCFGTPPHARVLPDQFAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q001</accession>
    <name>A0A5B0Q001_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_035939</name>
        <name evidence="6" type="ORF">PGTUg99_022850</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q001"/>
    <dbReference type="OrthoDB" id="20582at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019163">
        <property type="entry name" value="THO_Thoc5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13375">
        <property type="entry name" value="FMS INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13375:SF3">
        <property type="entry name" value="THO COMPLEX SUBUNIT 5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09766">
        <property type="entry name" value="FmiP_Thoc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="171"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1106531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1126270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="231" mass="26951" checksum="DD318118A339A76B" modified="2019-11-13" version="1">MDEEIPPIAEQQPANWEKIDAIADRLMLLANQLLESKLSRTSASSSTSSTMNPPEESFVDELEADENLILLAAPLFARLKSINRSSSSMVSSFKSQTQLIRNQVDQIHLDLQNLIYERRHLEKEIKKCQDFESEYQNISIHSLEEYFERNPEDKRDGPEEIDGHELMIKRLKFELSERKRFEAEKKELLQKKLKLSKENDEKKSKLDELEKQLDRFVVTAKEIQSKMANQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1R7</accession>
    <name>A0A5B0Q1R7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004895</name>
        <name evidence="4" type="ORF">PGTUg99_014936</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000010">
        <property type="protein sequence ID" value="KAA1137267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1R7"/>
    <dbReference type="OrthoDB" id="10296186at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366481" evidence="2">
        <location>
            <begin position="24"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="423" mass="49159" checksum="88A3B15D5C170F86" modified="2019-11-13" version="1">MTNTSWIMRITFVTLALLGTTRGAFPHLSATPALESSELPKRRRPDSIFEATFPTEDEPVRDDEEHRPKKPRLQCESSSASASGAGGEVGEMIHSSFHPREECLQHYAWITALQYPMGSKTLPNVLEDSFKFALGPGNPIRNLRDVIQHYFPGENNILQKLSKICAINEPYIYNRLTLWLKSVCALNLAFTTAFIEIKDKNDFSRELGRIFQWFAELLHYSSNLPVLEMKEDSNPKPFPALCNKLLIYLSNEERDQQGLHAKFSDQTFWKVKTYASAITHNISHSEALKTQLTVHFLGNYYKSSNEEKWNYIFESDESFAKLFSLMRYWEPRGKLQVRLERGRLFWISNHGDNSFLPWTDSLKFQDQFKMPALIDRHFYDAFKLDLDEEIRKNRLFTSNIDLNQELESSSYITPGIYLFLFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1V8</accession>
    <name>A0A5B0Q1V8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002946</name>
        <name evidence="3" type="ORF">PGTUg99_002824</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000010">
        <property type="protein sequence ID" value="KAA1137213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1V8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="109" mass="12094" checksum="4BB42EE6A2BB3999" modified="2019-11-13" version="1">MISLSTELKELGWIESDEPSKPHLNRSNTQAESSPPQQIIHSSPFSIRMITTKLNHVLMRPCARGSILEDFSTTLDERVGGPGLKDDDLVTIPILTLPSRSENELIDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5D4</accession>
    <name>A0A5B0Q5D4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5D4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022859544" description="Zinc finger PHD-type domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15964" checksum="85DD3711355F0A12" modified="2019-11-13" version="1">MFSISILVAAAMLLQVITASAIIHPSDTIASVGKNTLVKSGHKHTKRMESLREQLSGRCGKCGHGLDGGIYECSGCRVKFHSGCMWSGQQCNICIIKERIGQREVQKTQDENQRREESSSRYYLDEARNKYRNSDYRDKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6J6</accession>
    <name>A0A5B0Q6J6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Conidiation-specific protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6J6"/>
    <dbReference type="OMA" id="MEHRTEQ"/>
    <dbReference type="OrthoDB" id="5419162at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018824">
        <property type="entry name" value="Conidiation-specific_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052670">
        <property type="entry name" value="UPF0654_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36576">
        <property type="entry name" value="UPF0654 PROTEIN C11D3.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36576:SF1">
        <property type="entry name" value="UPF0654 PROTEIN C11D3.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10346">
        <property type="entry name" value="Con-6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="8514" checksum="F0282EA78EEA3D95" modified="2019-11-13" version="1">MANPGNVAGGLKATINNPNVSEQAKAHAEDRLEKEFSLVADEGNESKNPKNVIGGIKAAINNPNVSEEKKSELRSKLDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7A2</accession>
    <name>A0A5B0Q7A2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q7A2"/>
    <dbReference type="OrthoDB" id="10614143at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="250" mass="27877" checksum="9C14C81FB07869A0" modified="2019-11-13" version="1">MNLSGVVGRTQSVPPNALASPIQEATNRRKPPASLPSRVIKEDFDAIAAAKELLKNQELTTAPTLPQHTEAEEGEEEDLEVVVNAAPLAIKCEKALSAGQLNVIELLQKSKQIYFNAKDTEEYELLPHLVKQTIETFRLVRSFMTWQETLAKLERDGDVQQQVQGVQDSQELEEQREKGCKTRQVSDQLEEKRDGKKKMEESHSSSASYDQVQRHSQPLNFPPVASTSRVPIPPTPSVLTSSKNSEIHNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAQ6</accession>
    <name>A0A5B0QAQ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_017995</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAQ6"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000130">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF103">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSIDASE MNL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="245"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1110320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="498" mass="56982" checksum="2FF15D74C95F2835" modified="2019-11-13" version="1">MNLTHEYNYARTHVKAIDWSYVVGSHINLDSIYEGPQISLFEAVIRYLGGLISAYDLSGDDLMLQRAEDLADWLLPAFGTSSGFPVTHYQLGLNPNGKRAGRVVTADVGSLALEFTRLSQLTSKDFYYDNVQKIIDLFDGDQWVSPDRLGTLFPETVDAEDPESLSEKYSMGGMMDSYYEYLIKQHQLLRGRRSQYSKMYTSAIESARTHLIRTYDIESPGGKNFTVIGSMEAGVFTPTLDHLSCFAGAMIGLGARLLNRKGDLDLALKHTDSCVWAYESTQTGVGPEEAWLLGGNEYTRWKIVNYKGKAFRELHRSQDRGPTVMDSRYIGRPETIESVYYMWRITGDRQWQDRGWRMFTSWMESCVTEFGFASLKDVNRWPPVLSDHQQSFVLAETFKYYYLLFSEPDLISLDEYVLNTEAHPFRLESPGKPAKRYWSKPDESVNKAYEPPGINEGERGYGTFIQQWDRVDLDKISENDLQVYNKVKGIDTEDSYIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAU2</accession>
    <name>A0A5B0QAU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vacuolar protein sorting-associated protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">VPS20_2</name>
        <name evidence="8" type="ORF">PGT21_019507</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAU2"/>
    <dbReference type="OrthoDB" id="441172at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="189"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1110370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="242" mass="27221" checksum="C57A1CC0F489B2B3" modified="2019-11-13" version="1">MLCVLETSLSQPLNSLSSESMGQGRSSQARITNQDRAILDMKLQRDKLKQYQKKIETVLKLEEEVAKQCLLANNKPKALIALRKKKFQENLLSKTDQQLETLQNLVSSVEFSLIEKDVLFGLNQGNQILKEIHKELNIENVEKLMSETADSIAYQNEIGELLMSQMTNEEEEEVHKELEILQSLNQKVTDPQLVPHDEPPVSLSLPDVPTTALPAKTQTASPAEEPPNVDGTRVREREAIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBH7</accession>
    <name>A0A5B0QBH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBH7"/>
    <dbReference type="OrthoDB" id="2509438at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="91" mass="10672" checksum="F6BBD69B51371DA4" modified="2019-11-13" version="1">MVALMEPDNLEGDESTERMSVTNCSYACQIEEAQNAISQRSMERNIPYYAVPDALVIWNIRRMRLDPIRIHGWKTEHERRLQMSTFNNQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHF2</accession>
    <name>A0A5B0QHF2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Glycerol-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="5 14">1.1.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">GUT2_1</name>
        <name evidence="18" type="synonym">GUT2_3</name>
        <name evidence="19" type="ORF">PGT21_003755</name>
        <name evidence="18" type="ORF">PGTUg99_030202</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">21-0</strain>
            <strain evidence="18 21">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a quinone + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:18977"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Polyol metabolism; glycerol degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="5 14"/>
    <dbReference type="EMBL" id="VDEP01000407">
        <property type="protein sequence ID" value="KAA1088267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHF2"/>
    <dbReference type="OrthoDB" id="264015at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.870:FF:000001">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.870">
        <property type="entry name" value="Alpha-glycerophosphate oxidase, cap domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031656">
        <property type="entry name" value="DAO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038299">
        <property type="entry name" value="DAO_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985:SF15">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16901">
        <property type="entry name" value="DAO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00977">
        <property type="entry name" value="FAD_G3PDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00978">
        <property type="entry name" value="FAD_G3PDH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="678"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="764"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1088267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1112621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="815" mass="89387" checksum="357DB94574D686DB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIR9</accession>
    <name>A0A5B0QIR9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021857</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIR9"/>
    <dbReference type="OrthoDB" id="10313414at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022905266" evidence="2">
        <location>
            <begin position="23"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="104" mass="11548" checksum="3BF6AF9C1D21624B" modified="2019-11-13" version="1">MLIQISTVNLLSFIFTISLAMASEQPHNIAQFGDSSKTLENNANNSRKLSAAIADRDEAARQAEERRRRYGATHADTHRANQVYSYYDLEAQRAGAGRNPPAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QKN5</accession>
    <name>A0A5B0QKN5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_000339</name>
        <name evidence="12" type="ORF">PGTUg99_001760</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000033">
        <property type="protein sequence ID" value="KAA1106892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QKN5"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF67">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="521"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="744"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1106892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1113720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="786" mass="88539" checksum="2CB12144EA816658" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QND2</accession>
    <name>A0A5B0QND2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTD kinase subunit gamma Ctk3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QND2"/>
    <dbReference type="OrthoDB" id="21266at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070692">
        <property type="term" value="C:CTDK-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032786">
        <property type="term" value="P:positive regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042326">
        <property type="entry name" value="Ctk3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024638">
        <property type="entry name" value="Ctk3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28291">
        <property type="entry name" value="CTD KINASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28291:SF1">
        <property type="entry name" value="CTD KINASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12243">
        <property type="entry name" value="CTK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTD kinase subunit gamma Ctk3 N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="178" mass="20528" checksum="B3BF09CA12E422AE" modified="2019-11-13" version="1">MNLFEVRLQLVSILGKLSSSQDSIQSTIWFSLTQKDKYGEDLWDCLMEEAEKECMILLDSIDGLLLRLSTALLKSHQDYQYHKREQNRRMFWGRTSVSISQSLVPPSAAGLNLYGHLIKKDIIKFVETVVPTTFNKKGLLNLTSTLQVIKNWKPQVSLIEQFLKLVLLEQTDLLLNDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNP4</accession>
    <name>A0A5B0QNP4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNP4"/>
    <dbReference type="OrthoDB" id="2505542at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="237" mass="26813" checksum="6F1D272C822E9CF7" modified="2019-11-13" version="1">MYVDNNTDPNELLPLTDPEAIIRAGNAEKRRTKQLQSNSTAPLPTDPTTMSDETALTHETPGSTRTADASDMQIAKDRFKSVFNIQHAAIVEAQADRRQAAEDRRQALEDRRTYRQLLMSAHQANTARISRLEDLLLAMNVKNKTDVRPVQPAPGRVDLQKFRTSDGPIYRGPFQETKPFLQWIHGVQIFFNTKDVNNAADKIRIVGNLIAETNIQSFYANEATGFLAKSWEEFKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPH3</accession>
    <name>A0A5B0QPH3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031200</name>
        <name evidence="2" type="ORF">PGTUg99_033257</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000169">
        <property type="protein sequence ID" value="KAA1127257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPH3"/>
    <dbReference type="OMA" id="CKVACTS"/>
    <dbReference type="OrthoDB" id="2495782at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1127257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14115" checksum="113CD7BFCFCEDE15" modified="2019-11-13" version="1">MALERVSCTSCASAGFGHSCELDIALNSCKRCLQTQGECSYLSNSVERTMAMLQAISDQQDSLLHEIELLSARLANMALETEHLEWYERILSAQADDSIRTGHLFPPLITRTSLPGRRHKFVVYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPR7</accession>
    <name>A0A5B0QPR7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable electron transfer flavoprotein subunit alpha, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ETF1</name>
        <name evidence="14" type="ORF">PGT21_035037</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPR7"/>
    <dbReference type="OrthoDB" id="1715808at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000423">
        <property type="entry name" value="Electron transfer flavoprotein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="13">
        <location>
            <begin position="92"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1115307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="412" mass="43909" checksum="D34356125C86CA07" modified="2019-11-13" version="1">MTELRRRSRLGNRIFCDGSGTSDALSRSLDPPPPPPPPPPPRTTTTTTTTTTKIAHQSREMLSSRLTRAGRGALNASLNRAFSSALTRQASSLVFIEHRDGKINPATLSALNAAQQIDDNQIVGFISGSSKTLGFQKAVELAAKLPISKLLVKEDDSLERYVAEPLAPVITSIVKSRSISHLIGGSSSVTKNVFPRIAAQLDVSQISEVMKIIDGRTFVRPIYAGNAMMTVSSEAAIQVLTVRTASFEPIKMDENATGSVEVEKLKEEGEKKKTTGGSQWEDEVLAKSARPELGSAKRIVSGGRGVKNKENFDQVLTPLADKLNSAIGASRAAVDSGFADNSLQVGQTGKVVSPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADIGLVADLFEAVPELTKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPT8</accession>
    <name>A0A5B0QPT8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPT8"/>
    <dbReference type="OrthoDB" id="2506921at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="367" mass="41850" checksum="74DFE48862FFE37E" modified="2019-11-13" version="1">MDSKKNMSEEKKHWPQGDLVIERFQSLTNKYQSPRNEDSSPRGRAMQEDSTIDHVNMKKDLLTKLRSNLLPELGRQITDLVSPLDPTCLKEDPGLQLEIILAVQSELEGTLSQIQSAILTLCSEPLTSESIRNNDQHRKELKSFRATGLHYRITEELLPDVMYIFDESEELILQMKLSAKRDPQQPDIAYTRSQIIEYESFSLEGIRSSILWLEGSEFDLVQYHWPKETRGIDKQLEALLSLINGTAHLKEGSRMSGPLSDPAVRLSKSLLPIIKLSRLFLDKLSNRGMNRKRLPLHTEMRSDQLATLYELAGRVGCEISDVLRVLRTVDRPGDHFSQTPMVFQSKPTSRLGLSHGTLSSAWPFRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPZ1</accession>
    <name>A0A5B0QPZ1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_035071</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPZ1"/>
    <dbReference type="OrthoDB" id="8026949at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF7">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1115311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="285" mass="31384" checksum="E3E5893617601C51" modified="2019-11-13" version="1">MSGYRQIQNVSALIPTIEALDGTASVFPRWRSRIEDVLGMQNTLDIVKGTLPCPKDETKDAAPYSRPPDAKGYNPKESKEDWDALSDTACSLIRLTLTNPLAQRYRKVKPALRLFTTIVNTYEKNTRARRIRLQEAFWTAKHDPHKPIALWIGCVRVAADDLLSIGELPTDRQITDRLVGGLDLSWSAVRDSIVYTAVEMSLDDAVGALEAHEVSLNGSHQSDVVSAASAKHITCSHCGKRGHRLSECRKKTSSKTKAGAAQTVKLGGYDSGSFDDEDKVGVIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSA0</accession>
    <name>A0A5B0QSA0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000784</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000008">
        <property type="protein sequence ID" value="KAA1115823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSA0"/>
    <dbReference type="OrthoDB" id="2513458at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="119" mass="13524" checksum="C9862E153316F1B0" modified="2019-11-13" version="1">MSQIQGPLDVRITLAPIHIMWLKDQQSMINDILKKYEPAPEDQPSPLSHIDEYEQDRRAWDWHVLISGRVTAAARDMSIPEWAIPNVKAIWDARRNIYGKGPLLFTAPEAIPGQQNGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX20</accession>
    <name>A0A5B0QX20_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QX20"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022842192" evidence="1">
        <location>
            <begin position="21"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="258" mass="29422" checksum="5C3901BA97D8DB15" modified="2019-11-13" version="1">MANLIKRALIVIPHVLLILATPPSRSPLLEEALDTSRYLTDKNDQNRHTLHLSQVYSNFAHSQSEKGSGISDPELEILDGNRRLEGLKRPWLVRPREEEGNKVSDLKKQKYNKHYGLCGKDYDNVQHFGSFGPISKSQEYERTSALNFVKSDHACRQSKLEDQTFISCNSGSESSHKLTFYPSSRNFPCGIQNSGQNTLKNISEYYPNQSSSNEGQIVRGVNLKEIDPIVWAWIPAFWSQADKAIINESSEYYWNQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXB7</accession>
    <name>A0A5B0QXB7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXB7"/>
    <dbReference type="OrthoDB" id="10281001at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="483" mass="55193" checksum="C8E63B90B0F3ECC4" modified="2019-11-13" version="1">MDNYLFRASPDGTAETLMSKGRNPCGSDPIDHFASGSTPMDHVECISNTNFLPAFSSNAARDEGGMSSFVKSSQPVLLTSKQQVSHSEEERKFVAEEMDPSSSSASDKGIKRESPDRLKIYSHSQSSTLAEPMGLERRPHKVIKLLGFSIVTPADPTSDLEGITKHSKQSGESSSPVKSESDIGEVKPLHSFLSRKHKKISGSSQHIITPAACKIEPTKGSSEADPTSTNDSIRKAIKKEYQSEVLKFDEKVFRPSPISGKINQNISEILKILSVDLKLQEIVMTEKQFIRYCMFLYHNQDTNSEGILMNDDERRKQKLELLDKLMENRQHWYRHWLKRANLNLENFPENLNVKMRNLIVMYLFYVEMISTIVPGSTEVGSELTKAFEFAQTIYGSRASSHRIIDTEFYQKTLKIKKNVIRSISHTNLFPSLWNFLRLWMEAYRIDFLDLIIKNRLWDATKRFFNKIFVHSIENLNIQMQKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXS3</accession>
    <name>A0A5B0QXS3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXS3"/>
    <dbReference type="OrthoDB" id="2508646at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="159" mass="18031" checksum="77D5663FE7CCA901" modified="2019-11-13" version="1">MRPNSNEQAIGTTVEAHGDQSTGPKHNETAEGTAAEAHELFTDEELVEVFRVLFNSGNTGIFRSSLTSGKLNQIIQTLVSIYGFTSPFRMDYINTHMGKIPGHYAIESFHKQWCARGEFSRWQYYYPDYIAFQSLYDIDNTGGTESDGWTWTIIHQPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0H5</accession>
    <name>A0A5B0R0H5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mitochondrial 2-enoyl thioester reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ETR1_2</name>
        <name evidence="3" type="ORF">PGT21_012431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0H5"/>
    <dbReference type="OrthoDB" id="7482721at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051034">
        <property type="entry name" value="Mito_Enoyl-ACP_Reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981:SF2">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="141" mass="15772" checksum="79345C677D67A6A2" modified="2019-11-13" version="1">MGFKQNLIPTTAYNRLTRLPPLGSLASHHTLVKAITYTQNGDPTEVLKLREFTLGPPKANQLGLKFRLSTLNPADINTIQGRYPWKPTIRKDLIEDQEFYISGNEGLAEVTSLGSEVDPKQWKIGDWVIMVGGSNLLLIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0J6</accession>
    <name>A0A5B0R0J6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0J6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="55" mass="5874" checksum="8057565542BD502B" modified="2019-11-13" version="1">MLSGEKGGAGTARFAAHGPDRTPTGVQSDFTSTKPIQKSTGNDKPNLPERIPFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1B2</accession>
    <name>A0A5B0R1B2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_016325</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Regulates mitochondrial small subunit maturation by controlling 15S rRNA 5'-end processing. Localizes to the 5' precursor of the 15S rRNA in a position that is subsequently occupied by mS47 in the mature yeast mtSSU. Uses structure and sequence-specific RNA recognition, binding to a single-stranded region of the precursor and specifically recognizing bases -6 to -1. The exchange of Ccm1 for mS47 is coupled to the irreversible removal of precursor rRNA that is accompanied by conformational changes of the mitoribosomal proteins uS5m and mS26. These conformational changes signal completion of 5'-end rRNA processing through protection of the mature 5'-end of the 15S rRNA and stabilization of mS47. The removal of the 5' precursor together with the dissociation of Ccm1 may be catalyzed by the 5'-3' exoribonuclease Pet127. Involved in the specific removal of group I introns in mitochondrial encoded transcripts.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds to mitochondrial small subunit 15S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1B2"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF17">
        <property type="entry name" value="OS12G0638900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="5">
        <location>
            <begin position="424"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="767"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="768"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1119149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="839" mass="94421" checksum="72CADFE64C193F14" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2E3</accession>
    <name>A0A5B0R2E3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SGS1_121</name>
        <name evidence="7" type="ORF">PGT21_032011</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2E3"/>
    <dbReference type="OrthoDB" id="2496478at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="56"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1119697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="439" mass="49532" checksum="5E0C499536FBB011" modified="2019-11-13" version="1">MKESKPANRSRPGRTGVTVLKKLLEKNDEQLKLEIATHAKKFYKGVEAKPLQIETVANLARGRDTFLLAGTGYGKSRIAEIYHSLLPKTEKGVVVVLNPLDALGDNQVEEKLGSFSAINLTKLTFNATVANQIKQGGYNFVYLSPEIFMNNKMWDDIYFSAEFQDRLSLVVVDEAHMIYIWGVVDSSHGKSRSIQILVRHEDGGIFRPYYGKIGLHLQCRNNAPLLLLSATCQPVAIEGILNCLKLSRTTIDFFEGELVRREIRILRIKMINSLSSNLDLLNVYPSKNVTPDEDIVPTLIYSGTRARTYTVLEVLDMARGTPGSCGDPYNTFARRYHAVTGDKDKTEIIEEFSNGDFPVISATMAVGVIPRRRFLVNHDLNSLIINERTHQTEVKSVPKKLKKKQYFESNLFFDELLPASSPMLRPTYNRLDRTKNLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R312</accession>
    <name>A0A5B0R312_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R312"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022788802" description="HAT C-terminal dimerisation domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20503" checksum="0D97BD1E333CFF99" modified="2019-11-13" version="1">MVIFSLIFLIQGISLSIGLPTHTPPFVAKATSPTAGSGTVEYRISENHSDQFATCHVQSPKYDLYQEDGLLEEIDPNNHDEQNIRHEDLFFKGQSKEHPKVTFFPNGQVELKDGSYTHTIFPSLPLPKENWYDITCGLVKMAPVLARMSILPLVYFLSSEDETAFSKVKKLVVSKFKRPCKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0REW8</accession>
    <name>A0A5B0REW8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_025350</name>
        <name evidence="5" type="ORF">PGTUg99_013125</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000208">
        <property type="protein sequence ID" value="KAA1123455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0REW8"/>
    <dbReference type="OrthoDB" id="3367at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045013">
        <property type="term" value="P:carbon catabolite repression of transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051362">
        <property type="entry name" value="WD_repeat_creC_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107:SF16">
        <property type="entry name" value="AT02583P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="28"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="247"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="449"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="653"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="789"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="187"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="459"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="653"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="677"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1099931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1123455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="810" mass="86933" checksum="BD5B623914746923" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJE4</accession>
    <name>A0A5B0RJE4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020081</name>
        <name evidence="3" type="ORF">PGTUg99_022731</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000175">
        <property type="protein sequence ID" value="KAA1125807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJE4"/>
    <dbReference type="OrthoDB" id="2508980at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="25869" checksum="9C27E24771DC92E9" modified="2019-11-13" version="1">MAKQMCNLLVGNKGYQPGSWDPTSIHIRCFCHKLALIVNAGLQALSLKTLPPKREKASVLGFFPVLGKMVEEDEQDLSVQDLPVDVKGAKKTSPPINLALDSDRDYGNADNENSDKGPKESDENNTDHETTSAAHLQHAKTTKLKELTKELDVVIKQITRSAAQRSNFDLTTRKLNFKVAPLIAGYGIRWNICYQSHCKAIEACEVIDQILKDNQQHNSSEIFSNAYFSLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RRQ1</accession>
    <name>A0A5B0RRQ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001954</name>
        <name evidence="2" type="ORF">PGTUg99_012607</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000145">
        <property type="protein sequence ID" value="KAA1128029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RRQ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1128029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="205" mass="23499" checksum="2A1437066225E369" modified="2019-11-13" version="1">MHPTPSTSGPLDIPESKKIYRRVTLKCSKLAWNSLPATTQSKEGYTITLDGHIQRPVPENPYRLKNLKYNTPEEKHILDTKNLIFELRASNGSPDESKMALESNRLTMKDGTVIQIPQAEFDTIVSSTLTEREEQYLYFVNKSISPNPPIDTKEFLIMKEFEKRIRELRLLETDLKHVNVFLLDAAMSPPKLNQKYSEAIRTHGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RYC1</accession>
    <name>A0A5B0RYC1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_032300</name>
        <name evidence="6" type="ORF">PGTUg99_006503</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000111">
        <property type="protein sequence ID" value="KAA1130209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RYC1"/>
    <dbReference type="OrthoDB" id="2507313at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="334"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1081257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1130209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="395" mass="44205" checksum="E1CEFE2D25CA6871" modified="2019-11-13" version="1">MAVQHSPKEKNTPAYASGNGVDDLTGDSSRTSGNEITDAPFVRPAPPVRGDTYSPEELVLMTPAQLAFVQRHPNFWKTMTDNPNDLPGNQRPLAPVDVRMEHTARGVGDIKLDPDGMDIDQRQRQPTLQPYERLHDATALSAHLETNLGHVFRGWSYKNCPYYEGTIGVDVRRWLTTLTINLRSRQAHPDVWHLAGIRLLQGKAYADYEDSTVLDTAPHSWQVFCTWLISMNPISVSQNVIADEYDALSQGPNESCQSFYDRFRDWQCRAKIYDFPYHEETGFVSRLNKALNRKLTTLMHTEGRRGTPMSFSQIVVAALDEDRMYRKNAALAATVTSGSNKRSSDGGDSKPKKKASGALTCYNCNKEGHVSSKCPEPKTEKQKAYEAKAKPALKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S2Z6</accession>
    <name>A0A5B0S2Z6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019472</name>
        <name evidence="3" type="ORF">PGTUg99_033196</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S2Z6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366687" evidence="1">
        <location>
            <begin position="21"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="174" mass="18938" checksum="F9CCA034B6A805C9" modified="2019-11-13" version="1">MFLIQIIFALHLLGCYSVSAHPILSVNPLVKRAFPAASGVHLEQTISTGVSHAEAGLSSTHAFEFDTMGSNMGKADESLNVLKDGQELLPDSKKEGNLSGLNEEINKSPLITKEPNVEKAPAQTINHVDDELSASRINNSIDRLATVMSTDRNGVVRVATNPNNLNKFLFRRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SD00</accession>
    <name>A0A5B0SD00_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_012773</name>
        <name evidence="12" type="ORF">PGTUg99_022131</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SD00"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="501"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="609"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="656"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1085610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1136021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="707" mass="77580" checksum="A6BE0A1FB8081737" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SFV4</accession>
    <name>A0A5B0SFV4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016930</name>
        <name evidence="2" type="ORF">PGTUg99_032087</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SFV4"/>
    <dbReference type="OrthoDB" id="10463534at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1136123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="85" mass="9527" checksum="E791378DBF099F72" modified="2019-11-13" version="1">MASTELASDSKTMDSDSSNLKVSRIVDSNYLDWSFVVLLHLKSLGFSYVLDPVNEKERSAAYVKESVAVSSFIARKIHPTNLRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKI7</accession>
    <name>A0A5B0LKI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001668</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Puccinia</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
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                <person name="Henningsen E."/>
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                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Mdm12"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1064403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="152" mass="17528" checksum="52D27C7AAFD73630" modified="2019-11-13" version="1">MPKIVLSKLALDDQVKTILMLDKLPQNFHSLKTNISMNFESLPFDEVLKKLEDFAAQNQLNEDKKSSIPTEATMFTQSSNPEIICPHCKRGFRACSHCFKSGHTEDNCFQKHPNKRAPKSVTSSSKQHSSHMTRYTQEDEEYLQQKYPDLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLI5</accession>
    <name>A0A5B0LLI5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone deacetylase hda1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">HDA1_1</name>
        <name evidence="3" type="ORF">PGT21_009517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="56"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="229"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="276" mass="30479" checksum="167BD5F9F29FF24B" modified="2019-11-13" version="1">MGEAPPKLGCAMVASDVATETVDECLKIQSAFWKSLPRQGLSAKYELEMDGLSIPLGDVLKMHRSYDLYEAHGLCTIPSHDQQSSGLSKSYQNQIMVTDNVYDCKTLVLFMHDLGSLRNEIFTESANLKDENEALIAYNKVLMDWIRKNGYGLIDVQVLPSYLSDLVLDGMISAGFLGSTEEDRAKIQNTVKEYSYGKVIVSDKFRPSQILRSEWDKITEFIHAKLAGDLPSNGPQTNGNNLSRGTSQIASNGRQIPNGTPNGLRSEPPALPMDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LN82</accession>
    <name>A0A5B0LN82_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LN82"/>
    <dbReference type="OrthoDB" id="10316221at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="570"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="68"/>
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        </location>
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            <end position="104"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <end position="145"/>
        </location>
    </feature>
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            <begin position="262"/>
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        </location>
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        </location>
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            <begin position="576"/>
            <end position="592"/>
        </location>
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            <end position="612"/>
        </location>
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        <location>
            <begin position="731"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="770"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="864" mass="93169" checksum="31703CD1451DDDEB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQ59</accession>
    <name>A0A5B0LQ59_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_025003</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQ59"/>
    <dbReference type="OrthoDB" id="2506847at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6589" evidence="3">
        <location>
            <begin position="220"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="10"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1066206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="439" mass="49435" checksum="FC00E351914C123D" modified="2019-11-13" version="1">MPFLYNLVHSKLQVGRNGPSSTDDQDEDSESETSDNDTEEVEFDIGESKTVECGKTERAKAWVIKRNRKSYVMAKTICSMVAFGQSRRCNALQVWNSVVLLACGVTERVNSYLHYLGLTSSRKTGHRALASLSEEARKLLRDSLSVNSCDPLSPIICLDNIDFEEKVHSKSPEKTNHMFHGTWGYIHRIDRKILKDFDQEDFSIKRYKEDISRSAQLDVTPAMLIPSFEENEHFSQVIKSQIAQVLMKYLATGTDSKSPIALTPPPITQIKAQKPNIQMLKLMIASDNSAEGVGNMLEDIVRQSSLTQQQYHSELQLLEADLGSVLNLESLRSQRKPSGHVETNLGNTFMLLGAAHTLWNISKAIFLLHFGNRSNCEDLGAWSTLQALGLPSDRPLAKNDFTLMITNLRKVHEATLIHCLLYVISLLFYLTIRVSFVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRD3</accession>
    <name>A0A5B0LRD3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calnexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_023199</name>
        <name evidence="13" type="ORF">PGTUg99_002555</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the calreticulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRD3"/>
    <dbReference type="OrthoDB" id="1938156at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.250.10:FF:000001">
        <property type="entry name" value="Calnexin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000011">
        <property type="entry name" value="Probable calnexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF1">
        <property type="entry name" value="CALNEXIN 14D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00803">
        <property type="entry name" value="CALRETICULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036508160" description="Calnexin" evidence="10">
        <location>
            <begin position="21"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="231"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="513"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="243"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="307"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="524"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="544"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="132"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601580-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1066148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1072629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="556" mass="60983" checksum="8518A050D08A37D3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LW96</accession>
    <name>A0A5B0LW96_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LW96"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="11104" checksum="D8EE154D3A834637" modified="2019-11-13" version="1">MEAFTLSNIQIKKIPNYQPSHFFGVRQINNSPQTLLPEERLTVPGCRTTLMDHQQKATHPDNEWVKQVFSKAAARGLVDKDIVFDSCSVCQRKARSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LX04</accession>
    <name>A0A5B0LX04_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LX04"/>
    <dbReference type="OrthoDB" id="2507202at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="12735" checksum="4FBB8780CF611F5A" modified="2019-11-13" version="1">MLVTDELFSNEDDMGDLLQTVILSRYLNVRAPPVFHDEFDLARLFEMQSIDFKQSTRTTKEAFVWLLNRIYLHPVFHNNSFCPQLPIPHQLAISLEQLGANGNRASVGRCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZD0</accession>
    <name>A0A5B0LZD0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZD0"/>
    <dbReference type="OrthoDB" id="2498105at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="269" mass="29361" checksum="C328736A46E43462" modified="2019-11-13" version="1">MSVLRIPEEQPTCRCTASWDRRGSSLLGSGGLHLWNNIKPRLFPPKCSLIIPYPSKLSYPFHPKSCKSQPSSQSSAHVFISATMVAFNFGLLATLFLVIALVVAHPNNEGLVARDMSPEAKTKGRVSDKLDSLGKNVKWLNDAVQQQNLNSRVARERVAGYYQLFRDSFQYANDCGRLCFQSGSVVNVSAYKAFTQLDQLAKSVASKYGSGASTVMSPFSAYDTLVARTINGFAQEGTPAYNLLPPQFADSMAQVGFPQTAAAAHQIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M038</accession>
    <name>A0A5B0M038_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">A-factor receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">STE3_2</name>
        <name evidence="11" type="ORF">PGT21_013029</name>
        <name evidence="12" type="ORF">PGTUg99_008629</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000207">
        <property type="protein sequence ID" value="KAA1123555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M038"/>
    <dbReference type="OMA" id="IPPLIWH"/>
    <dbReference type="OrthoDB" id="2874149at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004932">
        <property type="term" value="F:mating-type factor pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14966">
        <property type="entry name" value="7tmD_STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001499">
        <property type="entry name" value="GPCR_STE3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097:SF1">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02076">
        <property type="entry name" value="STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00899">
        <property type="entry name" value="GPCRSTE3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1069134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1123555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="388" mass="43232" checksum="F881F399880958D4" modified="2019-11-13" version="1">MTPDFIAYLALSLVCALINIAPAASHLIQGHSGPASFGIWVVILNLLGFVNGILWMDDAQDKAPIFCDVSAKLTMVGPLGLLIANCCIIRYLARIVTPNPLVEENSTTRIRICTDYALSFGIPAFVAVLSVVFQVSRYEVEALVGCSNVSVLAWPTIFIFIIWSPIMCGIACGYSLYVLYWLIRQHRELSHLIARSRTPLNKSRFMRMCALAATYLCISAPYTIAGTVTILKDIGPFVPWKSWGYIHDVDNKFSDVRKNASYQLNIRDWLSVTAGLTVFFFFSFANESIAVYVKIARSLRLDNVLQSSSDWLCGRSNASRSLLKTRTDVSNHSPECVSDKQHQITFKPRSSFSQLPGSHHTRIAKESSCPNTIFVSVVKHTKPDFFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3B3</accession>
    <name>A0A5B0M3B3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026347</name>
        <name evidence="2" type="ORF">PGTUg99_016936</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000078">
        <property type="protein sequence ID" value="KAA1132629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3B3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1132629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="95" mass="10552" checksum="54F4DBBC51A047B3" modified="2019-11-13" version="1">MELMSRQLSSPLSLASTAGRRTLAMYPLIYKTKLVNLVHDRAVGLGPLRRPHLTLPTSGCSPTSHKEIFIGYWRQDPLQRASPISNKIPGAERLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6G7</accession>
    <name>A0A5B0M6G7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6G7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022702413" evidence="1">
        <location>
            <begin position="26"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="572" mass="66533" checksum="1DF74801F9A4F2FD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6L7</accession>
    <name>A0A5B0M6L7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022129</name>
        <name evidence="1" type="ORF">PGTUg99_011610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6L7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="131" mass="15615" checksum="59A9111D04B65B49" modified="2019-11-13" version="1">MDFHRTLILRGNAIPKKIQLPSVDVVVNLRYFPEATKRNPRARDPLTVQSFYQQAVNCEREERVHNRKLESIYMNCSAEMLVHQEKDWVFRNWLEPQRELGSFAQHIGALLSQFRHTHSTIFRICLLSWHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6P1</accession>
    <name>A0A5B0M6P1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014518</name>
        <name evidence="2" type="ORF">PGTUg99_015389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6P1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137159" description="Lipoprotein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="55" mass="5939" checksum="870EE4235CD68703" modified="2019-11-13" version="1">MKSVIAILSACVLLLASGCAAGTTPRYFQTCRGDEYYTMKTCAKDSAGKRKAHLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M765</accession>
    <name>A0A5B0M765_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="3">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">STT4_1</name>
        <name evidence="11" type="synonym">STT4_2</name>
        <name evidence="12" type="ORF">PGT21_026100</name>
        <name evidence="13" type="ORF">PGTUg99_014377</name>
        <name evidence="11" type="ORF">PGTUg99_027938</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="11 15">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000416">
        <property type="protein sequence ID" value="KAA1085185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000011">
        <property type="entry name" value="Phosphatidylinositol 4-kinase alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000012">
        <property type="entry name" value="Phosphatidylinositol 4-kinase alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000014">
        <property type="entry name" value="Phosphatidylinositol 4-kinase STT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045495">
        <property type="entry name" value="PI4K_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19274">
        <property type="entry name" value="PI4K_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="10">
        <location>
            <begin position="1423"/>
            <end position="1608"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="9">
        <location>
            <begin position="1681"/>
            <end position="1971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1066821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1072023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1085185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1997" mass="224691" checksum="9262C8790BA3EC2B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9B7</accession>
    <name>A0A5B0M9B7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_000805</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000161">
        <property type="protein sequence ID" value="KAA1072763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9B7"/>
    <dbReference type="OrthoDB" id="442352at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302:SF5">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="516"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="552"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="9">
        <location>
            <begin position="98"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="399"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1072763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="586" mass="63681" checksum="F6DC4278AAF64F5A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9V4</accession>
    <name>A0A5B0M9V4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9V4"/>
    <dbReference type="OrthoDB" id="10408790at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="84" mass="9917" checksum="8F5D205E1E95EAEF" modified="2019-11-13" version="1">MEGSPKAKIDEVEKSLGDIGQRIQELEARAKKFDEEYRKSDKKFKNKLQKIEQDYNESNRILDEKANKLFHEIENHYEGSSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MA32</accession>
    <name>A0A5B0MA32_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase</fullName>
            <ecNumber evidence="6">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_022546</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MA32"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF479">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023159949" description="Carboxypeptidase" evidence="6">
        <location>
            <begin position="29"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="49"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1073685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="409" mass="45389" checksum="A03D7877663E1935" modified="2019-11-13" version="1">MLLRGSPTALAQTITLLFSLLGNTTAWAVNPFQTLNQFSQNAAFGQRPGFQQLGVPQSFQNGQNGFQPGMGQQYSAQPDMGQQQLGTGQQYGSQPGAQSGMNPQYGSQSGQPATTGGSASTFPLRFSSQAAQKFYVDGKTLPFVEFDAGPSYAGLIPLSSDPNEKRKFFFWFWPAAQAVGANDLTFWTNGGPGCSSLESLLQETGPISWKFGQARPTQNQYSWTNATSMLYVEHPIGTGYSVGTPTATNQTDISRDLFNFFTQWLQVFPEMNGKNFWLSGESYAGFYVPYLADYIYSYQSNLNLKLMGMYLVNPTLSWTVLHEQMPIFPLVKRYEHVFALNETFMSTISRLHQECGYADYLAKYEVYPPPRGPFPAPEVGFYMEHQQSNSSCDTFNMVYEAAKLANPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCD2</accession>
    <name>A0A5B0MCD2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCD2"/>
    <dbReference type="OrthoDB" id="2142040at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706">
        <property type="entry name" value="SLR1338 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706:SF1">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28528" checksum="920FF022E2504754" modified="2019-11-13" version="1">MKNLFRHSSQKKNRYPPPAGPPPPQANQSIPQGQAGAYYGHPPTNSAVYPGAQNQQYPPPPGPPPPDNLAAPGYPANQFQPGLEDPLAMLSRYDTEMMWDETRDALIGVVEKAIQYDSDGIDLFFFNDAQQALNCNSPDQVRHVFRSVLPRRSTPTAASIKRVLDPYLTLLHQSKNGGPMVKPMNLIVLTDGEPDRGQDPEQVIVEIGRYLDSNRFPLNQLGISFVQIGNDPDNVITSDFILKALIGGVNRKIDHLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCM9</accession>
    <name>A0A5B0MCM9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCM9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="144" mass="15693" checksum="B0A1EC2BE4BFFA23" modified="2019-11-13" version="1">MHVHRSLIKSPSLRHSSHTIPGAAGATHEQPGSVGRIQFSICLRSYFEPAATSDDSRYFGCGPRFPVGLCGCKVDNHDNLYISEPPSQTVGGHLEWPWCPSGSCNTYCGNVLLSDASDQHLQDSYNGLPKTYIASNSIYRSGHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEC5</accession>
    <name>A0A5B0MEC5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEC5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="131" mass="14940" checksum="95138D7561B2511D" modified="2019-11-13" version="1">MQHPVLLTQQTTSNPLKRAINPNLSVTYPPTELNTPQQLPSYRLLWTLVPRLPCSVTRTREQLSHLSILGPYPFFQTSENSNQPQILNKKRNYNSERRPSSSKFRKTPSTSSIGLSHTVSCCRDPQLKPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHK8</accession>
    <name>A0A5B0MHK8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHK8"/>
    <dbReference type="OrthoDB" id="2506772at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="417" mass="48131" checksum="ED66177DFB1689D0" modified="2019-11-13" version="1">MLDLASDDSSSDEKVNDAPMAEDAQARKGKTPAKSKKPKPPETQLPHRLSSLTRSIVEFAKFMMGTTGRDNYHLPDPPTSEELKQYEAWGIDYVQAVDAFHQEQASRNDIEDEAEREHLKKKELKELELSLQSTRYEPAPIPPNYEISKHFKDQCDQSFRLKGFPRITFQWNKPSLEGSSWNFATADILANQWHSWYIKDRRYSPSTKLTVDAKGVIGRWLKTAAMNQAQKPKEEPSENSTRTTQEMNTLRRHREQRRIQIARHRHASAMRSVPKQHKEYLSELLSANDMSSDYEDDEDPEVPPTRILPFWRSKALTDLLHELDVATIQLASSEKKPNLIKLLQRKETREATELEVLQEKVPTNVCLQGFNKYFLQDTSILERRQLQIDETPKSYSLTQALVDLQKLTCKSNAVPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIL8</accession>
    <name>A0A5B0MIL8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIL8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="222" mass="24384" checksum="A78154DF1C51FA3F" modified="2019-11-13" version="1">MVGGSIPIPELSSRPLSHQPASIHLSSLPSNQAMSSGSESDRSSTQTPNVQQSVPHHRRTHSGQVAHPLPGTHTHASRARVQPYVPPLRGAAALAAANNGTTREHPRPARIVPAGQATVPPAGERVVPVTAVSGGRRARPWPLNVVPFEVTEDIIDDEYFEQLGATYDLRAPYTFFAEELVQWVDCYFWLLVLDSRASKHKLEVTRIGEVVQNRSQRMNMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIX3</accession>
    <name>A0A5B0MIX3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcineurin subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Calcineurin regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Protein phosphatase 2B regulatory subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CNB1_1</name>
        <name evidence="12" type="synonym">CNB1_2</name>
        <name evidence="13" type="ORF">PGT21_029500</name>
        <name evidence="11" type="ORF">PGTUg99_002206</name>
        <name evidence="12" type="ORF">PGTUg99_035867</name>
    </gene>
    <organism evidence="11 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="11 15">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. Plays a central role in virulence and antifungal drug action.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Composed of a catalytic subunit (A) and a regulatory subunit (B).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000455">
        <property type="protein sequence ID" value="KAA1077127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIX3"/>
    <dbReference type="OMA" id="DTNFDRD"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000047">
        <property type="entry name" value="Calcineurin subunit B type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1077127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1109855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1119575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="175" mass="19757" checksum="E39338E72A2476C4" modified="2019-11-13" version="1">MGQTGSQLLQEIEKSSNFTGDEIQRLKKRFMKLDRDQSGSIDKDEFLQIPAIANNPLASRMIAIFDEDGGGTVDFQEFVDGLSAFSSRGDREQKLKFAFKVYDMDRDGFISNGELFLVLKMMVGNNLKDQQLQQIVDKTIMEADTDCDGKLSFDEFKQVVANTDIAKQMTLEALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKI2</accession>
    <name>A0A5B0MKI2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002559</name>
        <name evidence="4" type="ORF">PGTUg99_027415</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKI2"/>
    <dbReference type="OrthoDB" id="2506503at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="278" mass="32247" checksum="EAA8FA6C5C6884B4" modified="2019-11-13" version="1">MKNKSNQTTNHQLINTLLNNQKLITIRLIESTIRTLIYFNRTTRRSRRRRRSRTAHVALIISLSIIRLIQSIKQTTTTTTTTTDQPEDEREESNQRLRSILESTNDSLEILINLLDILSPLNNNRLKTTTNLATLTHNILSYAHSSLEKAALWKLGRQTRLEIADTDQTIKANKLRIRSTLQPEHQRALIDQIHAHTRLNRARKITIKRCKRDLTRICWARIVFSIDAFISAHDLLELEYCSHGLRHLIDWTSALIDFRSAYWHALESLALANHSPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML52</accession>
    <name>A0A5B0ML52_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010727</name>
        <name evidence="4" type="ORF">PGTUg99_002374</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000293">
        <property type="protein sequence ID" value="KAA1111254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML52"/>
    <dbReference type="OrthoDB" id="786951at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039249">
        <property type="entry name" value="GPATCH11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032:SF0">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13821">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="102"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1111254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="313" mass="35603" checksum="BC89ED8D425E840C" modified="2019-11-13" version="1">MGPCRSNSSNSTRAKPVNLHDGQEADEEEEDFMSDKFLKQITETSTENKTYSEKRKRRLIDASTNHQKRSRVQREIEAREEGLSRNLIQANVVQEEKAPPESHSKAFQMMLKMGFQPGNSLGASASNGSNEPINIVPRSGRAGIGIAPKPVSAAKLPRLLFSVEGKMDLTEEETAQREAFLATSRTRFDGRKVDAILGRACRTCEELDRRRGLESNIFWIIKPSPQDESREDAHLTRLDRLVSLDCQNDPDNILEDLEDEEKQNWLALDPSTRLSCTLGYLREEYFYCIWCGCQYESLEELNKECPGEEEDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP32</accession>
    <name>A0A5B0MP32_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019597</name>
        <name evidence="3" type="ORF">PGTUg99_003165</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000248">
        <property type="protein sequence ID" value="KAA1118519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MP32"/>
    <dbReference type="OrthoDB" id="2500663at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="57800" checksum="FFD406F6645709F5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQJ2</accession>
    <name>A0A5B0MQJ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQJ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="108" mass="12169" checksum="5C6725349A10B600" modified="2019-11-13" version="1">MARWLPLRYTTSTRLYSLAGQEHYVLYPQEPLCSPLCRFLVGLLGFWSPDRWIIIGRPSRPSEPSPDPWNCSGTLDHAQGPLNADQKAPRPPKAVGSLSDDPKRPRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQM3</accession>
    <name>A0A5B0MQM3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQM3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022916351" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11771" checksum="6B7C712E43EC8CAB" modified="2019-11-13" version="1">MLIKKGLFIVSLPRLLHAQVLEIDPDLVNTTNCLHLVRRPENLSLPCPALYICYNGHVHVCGIQLRGCVQRCITCQHDSQQQYLENCQLGAAPHRRMPCPQAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRT8</accession>
    <name>A0A5B0MRT8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020672</name>
        <name evidence="4" type="ORF">PGTUg99_029623</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRT8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137301" evidence="2">
        <location>
            <begin position="23"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1124142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="101" mass="11192" checksum="52B4803ACC547253" modified="2019-11-13" version="1">MKTLWQVWLLQLSSVDVFLVRAPTPGQFGGLDHGDLVSDRLELSPPQIDSSSDKSPGTHHREIRTTLQPPDLNPSPSRQDQPRFEVSSSNSVEEIDFLGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRW8</accession>
    <name>A0A5B0MRW8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRW8"/>
    <dbReference type="OrthoDB" id="2509312at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="180" mass="19932" checksum="7761C1BC76077C81" modified="2019-11-13" version="1">MYHSQLSIRSFTFVVDAPRLGPNTMYQVPHATPWRCTGTSIDPIPHIKLNVLTPAPMESPPTQRNWLTFEGPITGVSSMGVIEIQTTSSNYYSDIPLSYRVPYLEVCGHVTIIEVVSSTQVTVSNPAAPQLNLFLTSDIDTIQEQLLNTVAGQSIWIMAKITNTMVLGLQLNNLLKAQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUB3</accession>
    <name>A0A5B0MUB3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUB3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="61" mass="7057" checksum="283150F606BB9431" modified="2019-11-13" version="1">MNPSNIQILKENKNQDTISKVFEIFSPPRKGRSQILMMFNYKQASPPSSTLTYRSSMNFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUE7</accession>
    <name>A0A5B0MUE7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase III subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUE7"/>
    <dbReference type="OrthoDB" id="2506837at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22303" checksum="CA438649927E702E" modified="2019-11-13" version="1">MKAVKIATGKTSRMRLRKLPATPIISSFSKVPQQLPLDFYSCKWILDLPTGQQRIIPDFSSVAFLPDPSNSLFARNHPNYDEDEKLSDRAFNKKYLDKAIKYYRMKEIVEEEETDNEDDPVEEDDEDANVQGEGVDPKTQNDANYLADGDWGNQYDEEEGETDEEGEDEDEDMEDHEKEGIDENYEEFDDVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXB2</accession>
    <name>A0A5B0MXB2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXB2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="205" mass="23387" checksum="A38FEBA45188825F" modified="2019-11-13" version="1">MFTSMKKIPGCSFLTLIVTQVQSHFTQLPTSSSEALKSTENNIPRHISTEQSIQPSDFHLFCPRELVKHSGEEVLSEIDFLSHWRQGTSNDGDPKNTYLQDSAPSLQTPSDGHVHYGSNGSILYFTITGPFQYSKVSSYFCLLYQYYNQCVSHQAWYSIGHFLWLQIAIYCKGLSFINHEVNSREPFCGQETLISSHRCFYQHPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N028</accession>
    <name>A0A5B0N028_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL42</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">60S ribosomal protein L41</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">60S ribosomal protein L44</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RPL44_1</name>
        <name evidence="9" type="ORF">PGT21_031135</name>
        <name evidence="10" type="ORF">PGTUg99_028821</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N028"/>
    <dbReference type="OrthoDB" id="2967263at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7 10" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1066468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1081923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="150" mass="17038" checksum="D4731A5B55CB75A2" modified="2019-11-13" version="1">MVRMGLSASAGCGQSSRYTDRAPQGRRHWATFGHTSKLSERLLIMVNIPKTRRTYCKGKQCKKHTPHKVTQYKTGKASLFAQGKRRYDRKQSGYGGQTKPVFHKKAKTTKKVVLRMECTVCKYKKQVALKRCKHFELGGDKKTKGAALVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6C5</accession>
    <name>A0A5B0N6C5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_016244</name>
        <name evidence="9" type="ORF">PGTUg99_016070</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="27962at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058565">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058567">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058564">
        <property type="entry name" value="TPR_TRAPPC9_Trs120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26254">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26280">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26282">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26251">
        <property type="entry name" value="TPR_TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="4">
        <location>
            <begin position="31"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 TPR region" evidence="5">
        <location>
            <begin position="449"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="6">
        <location>
            <begin position="700"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 third Ig-like" evidence="7">
        <location>
            <begin position="1020"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1084064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1093301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1353" mass="151658" checksum="92DC8FDE920C3BA3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBA5</accession>
    <name>A0A5B0NBA5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBA5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="316" mass="33121" checksum="E5D58C58C0974979" modified="2019-11-13" version="1">MSPDSCSADQDSKPDDNGKPNEAAADSKPDHNSKPNEAATDSDPNNNIALPDLGEEGLVIPAANKDVSPSGAPADSKPNETAADIKPIVQEGEILGPAAINGAKHDETAADIKPIVQEGEILGPAAINGAKHDETAADIKPIVQEGEILGPAAINDAPNGAAADSKHDETAADCKSNETEVDTNPNGGPDLEEGEILSPAANEPAIETDGLLWANGLFTDLGLATFLEDLKTTGCFQGPLTDGQFGQVLGQLRSTHGFSHLYTQYLDTRCVSTPKRLVEMLRQLWLDESAHGSPCHYPECIACHDMEFEVDDSGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDK4</accession>
    <name>A0A5B0NDK4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDK4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="143" mass="15134" checksum="1CE6B5F94AFA3171" modified="2019-11-13" version="1">MPPRPSKRAIRPPKLPSRPSSPPPYPPANSSQPSPSVFVSATHHASPLDHIPMSGLHIQSAAAQQSSLLPPILEMDWSASQSLPPIRYAPPDPSQPSLQSSYAPSWDSNPSTQSPPLPLSGNPHQQAVVGSGCWGSKCLHGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEK6</accession>
    <name>A0A5B0NEK6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018804</name>
        <name evidence="3" type="ORF">PGTUg99_034386</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000137">
        <property type="protein sequence ID" value="KAA1129431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEK6"/>
    <dbReference type="OrthoDB" id="10362728at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="173" mass="18751" checksum="809765F5F7A003D0" modified="2019-11-13" version="1">MAQATQPPVDSEDRRSSPLKSADQTRVHLKPYNNHHHFVEAGSEPRTASQLLSAYHPPPTSEPNTVIGSPISPLPPHHFSLHQVHQDIVASLPAGTHSLGTMANCGIHSFVKMDPSFDLNRPLSSISSDLKDGLNNHVRLLCLQGHPKFTYDILPDSLSAQAKNGYASSNSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGB4</accession>
    <name>A0A5B0NGB4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGB4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="327" mass="33455" checksum="0E6999ED44CD600E" modified="2019-11-13" version="1">MFQRRAGAPGSGNEEQGLTGDPKKAESGSPTDDQKKDGDKKEDEQKNDGGEKKDAEKKDAEKKDGDKKDGDKKDGEKKDAEKKDGEKKPPGEDKKEDATKNEADSNKEDGKDNKDLKDGAKEDGKKAGNGSAEKVPDTPEGIQQKIDAEKAKLAKLNQRVKAKEDRIATLEERLKRVGGGAPAGPPSPGPDPNNPGGPPGPDPNKAGGPPPPDPTKPVGPTGPDPTINGGGPPGPDPKKGGGTPPTGPKADTPAPLDPTKPTGPTGPDPTKAAGDLDNDAGDDSCAPETGDTDKNSSESGKADADKKPLDSGKADVPPNDQPGLGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJW5</accession>
    <name>A0A5B0NJW5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJW5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022689698" evidence="1">
        <location>
            <begin position="24"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="8335" checksum="AFB6004C0195A378" modified="2019-11-13" version="1">MKSISTIAIYVFTLIFFIGHAAAECTRHHKYYLCAYGDLWVPPLSDQCPKDSKAECCTNNLATDLAAQVDCVPYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NRJ6</accession>
    <name>A0A5B0NRJ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NRJ6"/>
    <dbReference type="OrthoDB" id="10587659at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023089701" evidence="2">
        <location>
            <begin position="26"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="133" mass="13337" checksum="DA7F5A3279F76515" modified="2019-11-13" version="1">MLKLKLSNLLIVVIAACQLFRSASCNQFHKNFKSQNLRRREVPFGNPNSNYANRNANDGYGPSTGRGNKVNNVPGQAGGAKSPGSSGPVPIGGNVVNAPNLGGILPGSAPGVANGGIGGSLPGVTNSGGINTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWA0</accession>
    <name>A0A5B0NWA0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019120</name>
        <name evidence="3" type="ORF">PGTUg99_018964</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000378">
        <property type="protein sequence ID" value="KAA1092099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWA0"/>
    <dbReference type="OrthoDB" id="2290122at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="96" mass="11235" checksum="13378223E9122558" modified="2019-11-13" version="1">MIVEDERDTLHDHVYNRISQSETTQPDATRSGDLLVFIWNYQTMRDSENHFALRDDLIAHLWTQKGQLEEDIIKEEEPLPYPDDSSELDKEGSTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0A2</accession>
    <name>A0A5B0P0A2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033144</name>
        <name evidence="1" type="ORF">PGTUg99_009931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0A2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="92" mass="9586" checksum="B28F9BED41B7B509" modified="2019-11-13" version="1">MPPLSPSPFLWNHNYLGTPTGSTESGARAVSQSYSNGSAQTTTASQSSKILMLLIPPAPSLSQAIGVFFFLACVLDGEFSASWAGMESLLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8J6</accession>
    <name>A0A5B0P8J6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8J6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="203" mass="23784" checksum="3136ABAD26CA3248" modified="2019-11-13" version="1">MPIATRACVRFWHKSLLRTSFGGSLREWRQPAQVTRPAEAWDLPQVRQLTSFGSGLTLEKVTSKLRPETHKLFAEKRCRRQEGKYHHRSSSDQARAARPNSYIFQNHVHLETRKTKLIADKRCRQAEDIWRPEMLYHMNSDRYAFKGKLNRLHERPTRGDFDYKMPLAQRVSLLQPSTAPAQIFPVYQLTRLKASTKNIPTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAE9</accession>
    <name>A0A5B0PAE9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAE9"/>
    <dbReference type="OrthoDB" id="10571081at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="225" mass="25850" checksum="506940C86A4A2F08" modified="2019-11-13" version="1">MIMIQSKKYLFCIVLQIMEASRLVLSAYDRCTTTGKLPLEESITPSETGVIRDHKNCLGPNSAIVGATQIEYRGKGLERDQGTDSDDELLENRKLTPMESDLTNIRREIRHLGKMYGSDHHMKLIGSLKQLYHYLFMEMGSTEESTYLNKWNELSKMLADRGKPDRNRPLVGGRLMFVLFLLGDYMKQYKPISSEFHKHIHIFQENTSADRQGIYGIPHPVQLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAG8</accession>
    <name>A0A5B0PAG8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAG8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="152" mass="16445" checksum="660C246E3A605B53" modified="2019-11-13" version="1">MCIPHIVNSRKNEKKGLQLIFILISFLDHSSLAGAPNFHPAGDWKPIESSQYHLPADHSPPSGAPNVLPVGDPKPVGYHLPAHSSLSGALNGRQTKGGGWKPVESPYHPICLNLVTHVRTKNECLKMLLDLDQPASSQAFSSSSATNFQGYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PDE5</accession>
    <name>A0A5B0PDE5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosinase copper-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_010376</name>
        <name evidence="6" type="ORF">PGTUg99_013713</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000348">
        <property type="protein sequence ID" value="KAA1098664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PDE5"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF126">
        <property type="entry name" value="TYROSINASE-LIKE PROTEIN TYR-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474136" description="Tyrosinase copper-binding domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="4">
        <location>
            <begin position="78"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1076529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1098664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="320" mass="36498" checksum="58A0324D83B49CDF" modified="2019-11-13" version="1">MRNQFWLGLCLLFLGSVPIHCLPGSGNKLRPRNSATCRNPSVRTSWTNLSASEKRAYQASVSCLLKAPSQMRYPGAVSRYDDLVYVHQRQSDYPNGRDEWHVTGQFLAVHRMYCYIFELMLRDECNYRGRMPYWDEQRDAGNFRNSEFLRDFGGPGDERGMVTQGPFANFEINLGPGFQNVRRRLHRQINETASAMAGPQYYQKLMAQPTFASFLNEIRQHSHIAGHNGVGGELGDVQTAAVDSIFFNHHLYIDWLWASWQEADPQARIFDLRGAGYETQANPTQETNLLTTLKFLGLAPDVPLYGSLDIQGGFLCYTYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PDN8</accession>
    <name>A0A5B0PDN8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PDN8"/>
    <dbReference type="OrthoDB" id="8963449at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="650" mass="73038" checksum="BD1386E3BEC98A85" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEI9</accession>
    <name>A0A5B0PEI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027973</name>
        <name evidence="2" type="ORF">PGT21_029973</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEI9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1100027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="242" mass="27943" checksum="ED243441661FA70C" modified="2019-11-13" version="1">MAWAAAQRQRNEVSRVGNLKGRITQVHIMRYTYLRGIKTKQADFGGKRTSDLTEDFSCKDFLSTGQFFNSLLIFQDFFSTPHREGWIDGLNSKVIGMLSCFYWSFFGFLSSVHHAEPIFIDLEKTLRPPGLTQSRVNDDEIENSPYLCSIQLANESFGISFILNSSLFLFKTTDFYFLSFVPQVGITRPMFGGTMRRAYERFMMRLLVQSRVVYKATATPREDEDADRYKHARIPRQLNTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHK2</accession>
    <name>A0A5B0PHK2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035523</name>
        <name evidence="3" type="ORF">PGTUg99_011647</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHK2"/>
    <dbReference type="OrthoDB" id="2507743at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="358" mass="39568" checksum="DAC5698CDA322EA0" modified="2019-11-13" version="1">MFGIINLQPEQTGEGAHHHQNNSNTRKRNRAISSESESEPTHHHQQTTDTTLDHQQPSKKRRKGVAGAILSTALDAALFTSAIGYAAYQFWCGKPLEQEQEENSFVQHPSPKLSIFTKSSSKIPTTTATTNTPATNLADHLEPPPPPYEPRQAEPPPEQLSAPLLRRPRLRSTTSRLHSARSTGPVTHPSSINFVLRPLHDPSLAQLPQSPPPPPPPPPRRSLPQNDELMADFVCSLDPYIPPLTPHHDEQEEEEDEDDDDPEMKAFKAQIQGLIRQGQSALASRPPTLSNDDLHHHPTTTPSPFAHTPALLNPPHLRNILPPSSKISESTTADSNLRLLHLALDKAAAKPASNWWEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJE2</accession>
    <name>A0A5B0PJE2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ECM16_1</name>
        <name evidence="10" type="ORF">PGT21_029431</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17982">
        <property type="entry name" value="DEXHc_DHX37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002693">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001555">
        <property type="entry name" value="Putative ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002147">
        <property type="entry name" value="Putative ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000045">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="434"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="779"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="217"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="342"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="712"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="370"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="713"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="729"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1101765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1328" mass="147372" checksum="95DB021336DC12FA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKZ8</accession>
    <name>A0A5B0PKZ8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_010410</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKZ8"/>
    <dbReference type="OrthoDB" id="428177at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079:SF4">
        <property type="entry name" value="GH26 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023130451" description="GH26 domain-containing protein" evidence="6">
        <location>
            <begin position="45"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="7">
        <location>
            <begin position="24"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="420"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1101188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="500" mass="54429" checksum="E9D4475110C860EA" modified="2019-11-13" version="1">MTERGGGHASRRSERIILGWPMKMLATNLLLLLTVLLLTGGTLAQQDPAAPAPSAPPRALNEHDGVYFGIWPDTAHGFSDTPAMINQRLGFNTSVFQIAQLIPLPTYNYTTGAGGPAPEYLIEQTQTDAAVFLTVYPTGLDVVTDDDLKLLGVQLQGYTEVLNRTVFLRFGPEMQGQWNPYGFQPTKFLALWRRMYSLVKAAAPRVAVVWAPNTPQSYPYGQLSSSLSPEDLALLDTDHDGKVSNTDDPFSPYYPGDDQVDWIGLSTYYKGPNFQNINVAQPSGYCSSVMNGTSPTSTFNWYEMYCTKPGKACMIAESGAAFHVDALTAQASTARSIVDLQRAWWQDCITSPSLVQAFPRLKLHMHFEHVKLEMDGGKPDLRDYRLSNDTTTLEAFKQDFSAVSAQFVVASFRALPAGVPPTGRPSGSPNSPVPVVPGQPGQPGQPKGIVFQTQRNPVVTGPPTLFGSRRSNAPNSIVNSGLLVLASSSVLLHLLLFPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLA6</accession>
    <name>A0A5B0PLA6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_017432</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribonuclease III family. Mitochondrion-specific ribosomal protein mL44 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLA6"/>
    <dbReference type="OrthoDB" id="67027at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19873">
        <property type="entry name" value="DSRM_MRPL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044443">
        <property type="entry name" value="Ribosomal_mL44_DSRM_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044444">
        <property type="entry name" value="Ribosomal_mL44_DSRM_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF32">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22892">
        <property type="entry name" value="DSRM_MRPL44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="9">
        <location>
            <begin position="199"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1101350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="579" mass="64457" checksum="BC15436ADF58FE89" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PM85</accession>
    <name>A0A5B0PM85_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_030105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PM85"/>
    <dbReference type="OrthoDB" id="2504711at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023064416" description="DUF7872 domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="3">
        <location>
            <begin position="230"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1101780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="349" mass="38102" checksum="FD23A2B9C3E82F2C" modified="2019-11-13" version="1">MSLLDLPLAIVALLSLVSYLECQPHLTPPSTSDSGTIPSNITTPSTVDSSITPDFRDPCAQLPLTPDLWQSLDLDNYLKSFPDVERLSLELFAEKAGATNFECGIGKICDANQICFPVRGRDWYILVAAQNWNSFSNEMYRAMTFALSIVGGLASSIVNDYAPKEPDIIMIDQQFWGILVGICGTIPGFAFPERFAYFGATIWPVIQDGTGLAANLLNTYHNVIATLPTDESSKTMDVQYLLSKAQKQVQAQLSNSMNRTISEGISTDKGLYGVLKGGVFLNDQFVTAERSEDEIKAAISVVARARLLAAVWKATNHFIIRGYKPCTQDGPNGALPDDDVFSYCSPDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PP23</accession>
    <name>A0A5B0PP23_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Signal recognition particle subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SEC65_1</name>
        <name evidence="6" type="ORF">PGT21_028367</name>
        <name evidence="7" type="ORF">PGTUg99_015011</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000035">
        <property type="protein sequence ID" value="KAA1136266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PP23"/>
    <dbReference type="OMA" id="NPFKEAM"/>
    <dbReference type="OrthoDB" id="2527451at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006617">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.30:FF:000010">
        <property type="entry name" value="Signal recognition particle sec65 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="218"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="252"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1101709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1136266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="264" mass="28676" checksum="DB2E673AF5B98345" modified="2019-11-13" version="1">MSRIYELSADDVDDFDFPLEASSSNMPGSSSGPSGSSNMPGMPPNIPGFSNMLAGMMGAGMGPQPPIKKEVDRSRTKNWETIYPRYLDSKAPCKTGGRRVALKYSLRWPLAQLIQHACTQIGLPCQLENDKVHPADWENPGRVKVLIKRNNKPIDPSIPNKYALLCKIGLLLRPLETVRLNLPPPTAKDRNSTPLPNINLRLPYNSPAVSHGFLAMAEAEAQKPTESSGPAIEDAPGSKNKGTKGPADTKKNSKATPKKKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQF8</accession>
    <name>A0A5B0PQF8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_000720</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1102942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQF8"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043456">
        <property type="term" value="P:regulation of pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051695">
        <property type="entry name" value="Phosphoglycerate_Mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517:SF1">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1102942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="23762" checksum="42671717AC7DBA4D" modified="2019-11-13" version="1">MLITLIRHGETDDNRNQIIQGHKDTPLNELGIQQARLTGQFLHQSLIGFDQIWSSDLQRAQKTAQVISEQIKDPPSLKTDPRLRERFLGDLEGKPREPRNSRVNSQSAEPPHSVLSRLLDFWNDQIINSPNTDRVLIVSHGGALRSLIESGLINRLGYQTPTDYSGDCSFANCSITTVEITSQSKSIKSIASVDHLQGKIQASNLNADDLNGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRD7</accession>
    <name>A0A5B0PRD7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRD7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="135" mass="14580" checksum="4DC63E05ED6E5809" modified="2019-11-13" version="1">MDYKRPTPCNGTQSLAGKPTNLPAGQTTPKTASVAALAEEELYSALDEAAVSAIQLMDYELELAKAEKKLAETHEAQPEKAAVNITEDNLFPEMDQAAVAALEDLDKHLATLRHEGSEELLDDEANHMANLPPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PU78</accession>
    <name>A0A5B0PU78_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016434</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PU78"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="173" mass="19063" checksum="8CCC1F3F73A4F131" modified="2019-11-13" version="1">MSVQRSPVNITTRAAAFRLAASQPVSDKDNETFMGARKVPDLGASTGPGATQTSDLDPARSDACGDSLDHRTENVLIRYGTARFSSYERFYLSNQRGHPLERQGRGILLGSVDPSIRFNLYDYTRSDSMLAATWDRQRDLSPTGLVADQNPPQSTSVESHPGNVMKNMMNYCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUQ7</accession>
    <name>A0A5B0PUQ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021515</name>
        <name evidence="1" type="ORF">PGTUg99_030788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUQ7"/>
    <dbReference type="OrthoDB" id="2505052at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="343" mass="39074" checksum="9C1E5F72EC47E0C3" modified="2019-11-13" version="1">MESANVDTYDDIFQSLLDIASSMEQQRKFAREFPGEHDILIGQDAPRRRLQLLLWHSKSELYTHAVELFAERQPLYRGTHIGTTLSTRIIAAIERRKSPIETAIRKYNEYRHEYLALLSPDEDQPNIPEMTYRTFVNLSLDDAFWQDVYLYHSQAPWAVNSDVRSGIHAMLITKRVEEEIGMIQHEFESALSWAVNHHVAIKAKISILENFLSEAELSEDDEEVEANDTAGGILTSISLGDCGNSVKAELSLYILKNHLTKHEALLNTWSADALSLWEQLYGNGPQTHEWFRLISTLASTEAVHTMGTEHPEQEVINDNEGVDIDEEGEEVDPGELLNHLHVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXZ2</accession>
    <name>A0A5B0PXZ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXZ2"/>
    <dbReference type="OrthoDB" id="10619686at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="290" mass="31761" checksum="41A4C263DF06C8EF" modified="2019-11-13" version="1">MLPDTPKPSTNPDSKVFYRFQIKPEAVVTKSEATSGVTLKLLQSHPTTSAAPVIAKAPLSSKPVKQIPKSSPLPDRNPEGIGETATLVSTSKGKSPSIFHPKRPRKDVVASIPPAISQTPCPKADRPILAQNLVKPLATSLSNPTNSPAATKPKPTLAPNTPLPPSTSSTRDLLDANHTLKHKGWKIPAEKVKATAERLSQRGSAGVWFWNSLLKVADYWELEEITNPLQAYRVARFSGYAIEGPLDHSPPPDNAMPPQFLKKIGAHSWFWYRDRWLLFSLHQKEITLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZE9</accession>
    <name>A0A5B0PZE9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZE9"/>
    <dbReference type="OrthoDB" id="3230070at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09276">
        <property type="entry name" value="Rnase_HI_RT_non_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="174"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1106295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="407" mass="43898" checksum="94092DC782DDEBB9" modified="2019-11-13" version="1">MSDSAEMARGYPNPNGYPDAARPSLATTHNKSKVETLANKVDRLAGIFSLGVFKSTSNAFIKARTTTPSFYNEAIRTSFSFFYRKIVFLKPNAGVRSFILQSRANPASRAADSARSGLATSRVDQALALEPENLHLTFDFGRTASRELEYVNLDATKEKAIESVKSIVSCHVSDPGDFLVFSDGSYHPEKGGARAAVCPSRNTFTSYRLGDNSHVSNHESEAAGVLAAIGLFKESCVGNAMRRVIILVDNQGVIRRTMDPGAPKPGQWFFKEIDKALADLPSCLKVSFVWCPGHRDILGNELADKLAKQALESSAGPTLRAKGNFKKVQRTALADLTASDKPQTPSGLHIASSSLINQLASGSEVGSEEESMEAQNFVQGRDARPGPQMLTGGGSNLRLPSQFKPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2E5</accession>
    <name>A0A5B0Q2E5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_014393</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CYC8/SSN6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="418911at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000403">
        <property type="entry name" value="General transcriptional repressor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000078">
        <property type="entry name" value="Transcriptional corepressor Cyc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF1">
        <property type="entry name" value="LD02225P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="422"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="490"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="527"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="564"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="601"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="673"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="707"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="835"/>
            <end position="1848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="205"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="289"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="806"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="837"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="909"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1064"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1171"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1238"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1272"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1373"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1412"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1438"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1455"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1484"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1503"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1525"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1546"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1606"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1682"/>
            <end position="1697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1710"/>
            <end position="1722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1765"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1799"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1821"/>
            <end position="1840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1107450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1874" mass="201748" checksum="2A418BC6390BADBC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q675</accession>
    <name>A0A5B0Q675_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_019530</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q675"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF627">
        <property type="entry name" value="OS06G0192800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022901135" description="RING-type domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="159"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1108623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="378" mass="42683" checksum="D060FC17E862EA67" modified="2019-11-13" version="1">MLLLVQILWLVVLFQVSTLFSYGLPSGPGATSSSGNWKKLRQSYKSLKRKCSLKSGLSGSRRFDSSSLNPTAGPEAMPVCYICSATILPLQDMFMFPNCGEGHTIHQGCVDAQYFGGSSCPICQAVSSSIQQHELVAPSSSIAQVKDQQMSESQEADGCPLCLEEWSQEDERLEWPICSHFFHKTCVEPWRRSHVTCPVCRREDPELLLKRSNRPGLVETTPQPDVSYLLDQDSSYDMATHSSYPVARDSSYHMAQDSSYQMARHSSYPVARDSSYHMTRHSSYPVARDSSYHVARDSSYPVARDSSYPVGRDSSYHVARGSSYHVSEDYFMDRSREIVRTLRNARCVEEYEGHEQHTFSPFLLMFSFGLVFCCVHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7A1</accession>
    <name>A0A5B0Q7A1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Homeobox</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NKX2-3_2</name>
        <name evidence="8" type="ORF">PGT21_033075</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q7A1"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051892">
        <property type="entry name" value="LBX_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24336:SF8">
        <property type="entry name" value="LADYBIRD EARLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24336">
        <property type="entry name" value="TRANSCRIPTION FACTOR LBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="269"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="271"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
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            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="RuleBase" id="RU000682"/>
        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1109098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="355" mass="39823" checksum="6B8AC05BB9C36510" modified="2019-11-13" version="1">MNLGNPASDDFLRHQFDFLNTNSQTELLSYDYNQPVMVSSPTCQLSETDSFLPFENSTQTSATYHQMSLPNPNIFSKATESREISPFMPLSQEAACGLAVCSSASQPLPILSPPLGLDYYTLETSRAAPLIGSKRSLPIMNRGEMSQNAHEIKESLLGFEGRGGPGFMHDFNVLPNTTSKYPIESFHETGESSSHLSWDMRTRSSSSRSPSYDQSSKFSPHNLPARSGAYQPEKSTTSGKKQNSPQVSGSRDYQTNQKRVGSLIDIHHLTNHVPRRRLTVEQEQILLRRFAVQEYLSKREMEELAEKLKIPTSQLRTWFGNRRSKLKAAARQKAKMEAGMGHLTDAELRSLNKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBM6</accession>
    <name>A0A5B0QBM6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBM6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="106" mass="12224" checksum="5257F4360F3CCD0C" modified="2019-11-13" version="1">MQSTGRRRRDSTKHIWYAHASSLQAIVLESRGSRGFRVVLALATQTTSHPALLLRAEVRFGTCGNRVYPNFHRHKWHTPRSDWHPPASTTPWLKRPFEPSISTPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBQ0</accession>
    <name>A0A5B0QBQ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBQ0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="16200" checksum="4B31BE476F3DAA63" modified="2019-11-13" version="1">MLNEGRIDEKQRSRAKDQVTPIGGLVRVLITLPSTFHPESSSRPALPPISDDKAMFLLAILVLVEFNDSCFASQYLRDRSYPPSITDVLVIIPTKRENFWETNRTPTNQSRRTRDNRMHARKNEQNKYKPWRAYFHADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC29</accession>
    <name>A0A5B0QC29_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021897</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QC29"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="152" mass="17520" checksum="0432A04482A68ED5" modified="2019-11-13" version="1">MKLDISDLLWQELTSKYEPNIARFITSLSSNVDPFRHERIPGLTPRMRDLQLTDSIQEFAHLLWCVNSKFLRTFKASDKAYLQEQLAVQDWINHLLGKGAKSERVSREKVQRKATNSDGTEDTLRKCMQKATSDEPSHSKGYGILLQKQKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEJ3</accession>
    <name>A0A5B0QEJ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_003655</name>
        <name evidence="10" type="ORF">PGT21_004100</name>
        <name evidence="12" type="ORF">PGTUg99_022099</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEJ3"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000651">
        <property type="entry name" value="Poly(A) binding protein cytoplasmic 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000933">
        <property type="entry name" value="polyadenylate-binding protein 1-like isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="28"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="116"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="208"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1067407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1111542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1137813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="306" mass="33724" checksum="11EB30343903E831" modified="2019-11-13" version="1">MSNPTSVESTASAPLPPQPLSQPTPAEYSVYVGDLPPEITEEQLTQVFSQASPVEKIRIKLPKRNTRFRRAYAYVTYSSADAATAAIREFNQTEFLGKPCRVMKSIHGAAKGLSEANVFVKHLPAKLSAIAFHDTFADFGEVLSSKLAIDHLNVPKGYGFVQYATAEQAQKAIRETNGSTLDVEGATKPIKTSLFIKKENRIVSREFKNVFFRNLSTKITLEKFKQTWSKYGVITSAFLTLGKDGKPTGTGFANFEKPSAAAKVVKVTQKKKAPGRVHAVRALTRSERERYKSRKAAQASEVAAQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFF1</accession>
    <name>A0A5B0QFF1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_013465</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFF1"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF1">
        <property type="entry name" value="AMMONIUM TRANSPORTER AMTB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="8">
        <location>
            <begin position="49"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="474"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1111839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="502" mass="54407" checksum="35BC877D594F307F" modified="2019-11-13" version="1">MPLLTYGSLSESNSTMFTGPESILSTKGTDVIALESDGQIYAYDPGDIAWVLICAAMAWLIVPGFAFVYSGMVRGKNALSLLLLALLSMAVVSLQFWFWGFSLAFSHTASLFIGNLDMVGYTDVFSEPDIIANNRIPTIAFAMVQQMQASIVGAVAIGSAAERGRIGPTIIFMFFWATLVYCPINCWIYNPNGWAYKWGVLDFGGGVSMELCAGMTGLAYSLFIGRRRGYGIKMHPHNVPHVALGTTLLWFGWMGLSGSGTLANIRGAMVMINTHLAACAGGLTFLVMDFRLERKWSLVAFCLGAICGLVAIAAPGFIGPSSSIVVGIFAAAVSNFTVAYRDKLPWDDGLDIFAGHATSGAVGVILTGIFAQKSIASSDGFTVIDGGMIDKNWKQMYKQLAWVGAGGTWSFVITYLIMIVINRIPFCSFRVDSESEVKGIDEDQSGETAYAPDSYMFRRPPTMIDSKRGKKFRSTFGRFSSHPGNQSLSPKRPKSVEKPRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHS6</accession>
    <name>A0A5B0QHS6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHS6"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="1">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="208" mass="22612" checksum="3B6B1F9AA1CD0F6F" modified="2019-11-13" version="1">MAQVVDEVRIMAYDVHGSFTKTTGPNAPIRAQCADPENQLSVESAIEIYLKQGFRPEQLSLGIPGYAKSWTLTKPELTPRIVGNYTSYYYQNYTGIPAGGKFDDQPGLDVCGQKTGYGGSWLVNELVDAGFLSDDETSGGKGFTRYYDACSGEPFLASNSTLISYDDTESTVSKVIYAKSKGLAGVFFFDTMGPRDKTVKAARTALLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHT5</accession>
    <name>A0A5B0QHT5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">H(+)-transporting V0 sector ATPase subunit e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">VMA9_1</name>
        <name evidence="10" type="ORF">PGT21_003190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase e1/e2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHT5"/>
    <dbReference type="OrthoDB" id="1508846at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008389">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05493">
        <property type="entry name" value="ATP_synt_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1112599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="121" mass="13595" checksum="3AACDA6FEBECE11C" modified="2019-11-13" version="1">MGGWLIFNRSHPRRRSLVSWDGCQLPKEMTKIVIRTAVIATITCCYLMWAIVYLAQLHPLIRLNLKRGDLRPNIDAAPQTKKPGSIISAANFHSSSSSITSCPLRSGRFQGIIDFKLDRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ54</accession>
    <name>A0A5B0QJ54_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rho GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RHO3_4</name>
        <name evidence="10" type="ORF">PGT21_025107</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ54"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1113208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="234" mass="26208" checksum="B4DADFB669092FAF" modified="2019-11-13" version="1">MTDLCLKPSNSIIDSISTFGQKKIISRKTLVLGDESIGKTSLLHVYARDHFPQVFEPTVVENHTIDVLVGDQVVELSLWDNVDLSGQDEIGKAGPSSYANVHVILLCFSVDKPNSLTSTENKWLDQIIERCPGVKICMAALKCDLRDDPETRAHLAKQNQRPVDYEEGLATARRIRASRYLECSARQNRGVSEAFLEVAKVSISTKSTSVYNLIRPGYRYFKRDRSSSRSCLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ68</accession>
    <name>A0A5B0QJ68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ68"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="84" mass="9523" checksum="543B4210CC6D360E" modified="2019-11-13" version="1">MPLAKPIRANRRKKHAANPQERVVKLKGDIFQLTTILCEFLIFLSVTFMPALEPDRCDLVPQPTRFGGFGYSKTSPRRINLVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QK63</accession>
    <name>A0A5B0QK63_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquinol-cytochrome c reductase subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QK63"/>
    <dbReference type="OrthoDB" id="2391627at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019182">
        <property type="entry name" value="Cytochrome_b-c1_su10_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28254">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28254:SF1">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09796">
        <property type="entry name" value="QCR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14009" checksum="34790A15CFD96BB2" modified="2019-11-13" version="1">MLLSGFGRFRQTHKPCLSSAQPTNNHQPSLQRNPSNTIDLKRIKMARPSATPRPRFAPNIKPQPHLMGVTSSVLSRWAPILGIWGAGGAAFVTLAASAIPRFQEDVLKKIPGVASYYESTVPDCDKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMY9</accession>
    <name>A0A5B0QMY9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_014073</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMY9"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF3">
        <property type="entry name" value="RING FINGER PROTEIN 141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023074471" description="RING-type domain-containing protein" evidence="7">
        <location>
            <begin position="16"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="130"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1114571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="240" mass="26449" checksum="C5B1E488A132508E" modified="2019-11-13" version="1">MDIVFLLLLAGVCFARELPDGSSLIEFREENTNFLPSLHSIDIPSAVIQRGSDGSSSCHVSAGNADLQGENSSLKEIVIQDGAGGRSPGKEVPTEISNSEDSPHYLKFDGGQVASHDLKLSGNAREAEMCSICLNSIPSTQDPVFYFGQECRHNFCNECIDNWLNQQRKQATCPVCRCPVEWTKELAAKLPAEELQFDQPCRSMMQIIRSLSREQVAILTALVLFFGILITIGQIVPNMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN72</accession>
    <name>A0A5B0QN72_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013258</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN72"/>
    <dbReference type="OrthoDB" id="2508841at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022696014" evidence="2">
        <location>
            <begin position="22"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="234" mass="24922" checksum="B7D5B48BE1830810" modified="2019-11-13" version="1">MFINIPFISLVALVSCASILGAPGQDSSRYTLSRRQATPTNPNGMKNATSATPTKMMIQMTTTGSPAQMTQQQKEAQEEAEEKMPGLNTISNAMNELYFHEKFGSQRNEPMVLLKMVGQAAGATGKTGDPEAVSKAILDTYAPYNQGKPSSITGSPITPLNKNMAPIEETPSLKTVAHSLHETWEKLMTMTPKPDNALLVPVFCQAIAASSTTGDAAQVTKAIDAAYAKIAGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP23</accession>
    <name>A0A5B0QP23_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP23"/>
    <dbReference type="OrthoDB" id="5983572at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="76" mass="8276" checksum="230CCAD0D88A7505" modified="2019-11-13" version="1">MELKFLAPIKLDLAINSATLGIDEPSEAANCNSPAYQQSEIKKPILRVAIVDGTDGVEQVEEDSFTRRRKSLSISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPI0</accession>
    <name>A0A5B0QPI0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mob1/phocein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033441</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPI0"/>
    <dbReference type="OrthoDB" id="10262609at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28964" checksum="CFCC7F5DE6B90470" modified="2019-11-13" version="1">MTNNPPEEASNSSPSDSSISSPSPPANSERPTHTTGDGQDPANDCRAPIRLRKGTRYEDVYPSTLASSENNHGPSSSLHLASPESPFQLQEYIADLVRADPHAVDRIISPPSPLIPREVWVYEQLRRIALDLSYPLVCALQTDCNRDKCPEMKAGEWLYLCAAHATANENQCCAIDYIVHTLDGATALLNSARYFPSRLQIPSSSNKHFTSIARRLYRILAHAWFHHRELFEECEVNRPSPPIYTHTPIHRSFIFVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPI7</accession>
    <name>A0A5B0QPI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPI7"/>
    <dbReference type="OrthoDB" id="2506296at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="248" mass="28944" checksum="7B5EC1BC53A2C6AF" modified="2019-11-13" version="1">MLRHPRALSKIIKITHHGQSHTSRIYSQLPSPYRPSIQIMRIHNHTKVNNQEFTGKAVSRDATDLPQNEQQPSDHTDLRTSKTMKTSTRFWDNKSLLCVRERNDDDLKTSTASEEKAMDFNPAVDAMNAFRIATLIVFGLTVLTIGGLSKRWELDGWEDWRIFIQSGHLGKLLIPENWSRSASQAIPDRLRPTPVPLSEQELLDELELISSWSWKKRVDREWEVERNRREAERLQWEAQRKALGKKIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QR25</accession>
    <name>A0A5B0QR25_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_035131</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QR25"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1115314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="401" mass="45147" checksum="CB8D4285956AFE07" modified="2019-11-13" version="1">MYEDSTNGRLARLTRCNCPHFIRLGSSCKHMYHIARVHNLLVVEKTEQPGQNEHMPTSHTERLPTNQAQTNGLTSNHPLSYLSTLTFIDFTDAGAPLPTLDASWKDPIDHLISAHTYPSHCTTHKSDTIRADAPDSNTLVPATELDAAQLSQADNLFEVPAHDETRIMNHQRPTRLVKRSNPEEPESSPQSTSKRPRVWRMGIEIEGPIVTAAAQLVGGQLIDISHNTLLCLPNSTETIMIDRQISEDDRWCLNQKAVASAMVAVVAMAEMMKKKKHRTEIVDNCSPQAMETYMHSLHVILRMVEESIPGGILTPRPDIYGVGEVGKMKKGEVGKLMAELLSVSWKHLKRTDLLLSELRNRMGLWKSTTTRYCELFKQRCHEARSMVEDYCPSSEGRVQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QT79</accession>
    <name>A0A5B0QT79_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016681</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QT79"/>
    <dbReference type="OrthoDB" id="3207600at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="309"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="391" mass="44100" checksum="D0714F7479211E2B" modified="2019-11-13" version="1">MATYWGSLSGCTSTVQLLDSIRQLMNKTVAGKKLWKAYILKEAKIIVNSEQPNGGEFLKGSFHKSSSISVSFFEKVEKEERDTKLVQDNMPFLYNLVHSKLQVGCNGPSSTNDQDEDSELETSDNGTEEVKFDIGESKTVECGKTERAKARVIKRNRKSYVMAKTIFSMVAFGQSRRCNTLKVWNSVVLLACGVTKRVNSYLHYLGLTSSQKTGHRPLASLSEEARKLLRDSLMVNSRNPLSLIICLENIDFEEKVHSKSPEKTNHMFHGTWGYIHRINRKIFKEFDQEDFSIKRYKEDISHSAQLDVTPAILIPSFEEKKHFSQVIKSQIAQVLMKYLATGTNSKSPIALTPPPITQIKAQKPNIQMLKLMIASDNSAEGVGKVLEDIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTE3</accession>
    <name>A0A5B0QTE3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTE3"/>
    <dbReference type="OrthoDB" id="2506685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="656" mass="74560" checksum="252C1E56E3E43A77" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXA8</accession>
    <name>A0A5B0QXA8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXA8"/>
    <dbReference type="OrthoDB" id="10558244at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="109" mass="11632" checksum="2B849C373A1DCCAA" modified="2019-11-13" version="1">MSHPDNPAWLSSASQRRRQAKEEKKTSAPMEPTKRSSTPDAGPTVVVAPKQSITPNVFHTDAAPKRSSTPDAGPTVVVAPKRSSTPDAGPAVVVASAVFPVWNSYRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXW1</accession>
    <name>A0A5B0QXW1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ferric oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_021050</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXW1"/>
    <dbReference type="OrthoDB" id="3944240at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF28">
        <property type="entry name" value="FRP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="8">
        <location>
            <begin position="152"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1117715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="326" mass="36769" checksum="CA8C7C6AC278800A" modified="2019-11-13" version="1">MASPAAPPAPGRTPFIPPDYELNEHIADFIGYLTLAVLCRLKLCCFIKYRLRATSRPASFIKKGYLLSKNRNETGPPNGGKAQPFADGSPLLRTKFTLIKLYNLKVPYMSALVNHFNLGNCLFLLVYLILLVILSLYKNFEGTRSLENYKRFGLIAISQFPLLFVLSMKNSPLNHITGVGYERLNFIHRSIARIIFIFALIHGALQMNLQHNLTGKIHFKKAPKYGLVALCLLALLNIMGSRVFRNSLYQVFLTLHVFGYITLVVILWLHSQHTRPFLKASIAFLVLDNLLKVVKSRVKRATFTAMPGGLTRIEVHGINDGWRAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZ72</accession>
    <name>A0A5B0QZ72_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kinase subunit of RNA polymerase II carboxy-terminal domain kinase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CTK1_5</name>
        <name evidence="10" type="ORF">PGT21_032025</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZ72"/>
    <dbReference type="OrthoDB" id="204883at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008024">
        <property type="term" value="C:cyclin/CDK positive transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07840">
        <property type="entry name" value="STKc_CDK9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000588">
        <property type="entry name" value="CTD kinase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000562">
        <property type="entry name" value="Serine/threonine-protein kinase bur1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF546">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="927"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="359"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="530"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="893"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="40"/>
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        </location>
    </feature>
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            <begin position="77"/>
            <end position="112"/>
        </location>
    </feature>
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            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="200"/>
        </location>
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            <end position="691"/>
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        </location>
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            <end position="780"/>
        </location>
    </feature>
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            <begin position="781"/>
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        </location>
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        </location>
    </feature>
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        <location>
            <position position="956"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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            <dbReference type="ARBA" id="ARBA00006485"/>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1118125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1259" mass="144487" checksum="C4E0C25C03D0BDB3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R075</accession>
    <name>A0A5B0R075_PUCGR</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010654</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Basidiomycota</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="KAA1118932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="83" mass="9271" checksum="8F3281A74499FE79" modified="2019-11-13" version="1">MICGELSHLSENRDAAVVGFLRLSLEIDGYKRPISKDRTKKNTISVKDGRESHQAAIPMVPGPFSLFFSDSRNKKRLPVNAMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0C9</accession>
    <name>A0A5B0R0C9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_011073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
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        </lineage>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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                <person name="Miller M.E."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="148" mass="16877" checksum="B27F5357CBDCEBC0" modified="2019-11-13" version="1">MQGNAESHRHPNGKIQPMPDPRMATRLRLRRTRQTPSQSLRMNSAIILARFQLRSHNQEGVDSRIRKPLDRCDYHHRFDRSDFCVPGWSPQCGKGDERRAADLVVGRTDQSELRTTCHTSPDHDTSGQTSLHVLLADTELINQDPATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0Q7</accession>
    <name>A0A5B0R0Q7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_012067</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0Q7"/>
    <dbReference type="OrthoDB" id="161814at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1118979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="300" mass="32801" checksum="3FD83C1C88B145BB" modified="2019-11-13" version="1">MSSLPSQATTTTTTTRRAAVASRLKPSIAQSIIRPQNLSTNIFTSSTNKKQAKTNDDQENNKQPTPSANPAAAFSSSSQFPFSFPHLAGLGLAMASNTLNSNDHHHHHHNMEHSSPALEPEAMNHCSMNMVFNSQPIGTCIVFRQFYVSGTMALLFYLSLLFVIAVGYEYLRLASNRFERMIQVKLSGGASKRALSPPRRVSSNENLIDNPVASLADGSNLGLINPKLASWGQISVPRSIQLTRSLFYVSNVALSFFLMLVVMTYNAQIILAVLAGAFVGHFVFHREISFGQPDKGMACH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1R8</accession>
    <name>A0A5B0R1R8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <end position="242"/>
        </location>
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            <end position="351"/>
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            <end position="449"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1119005.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1048" mass="116498" checksum="88D36DF808CD7C89" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0R213_PUCGR</name>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="334"/>
        </location>
    </feature>
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            <end position="729"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <begin position="275"/>
            <end position="286"/>
        </location>
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            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="697"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="719"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="813" mass="92398" checksum="2C0FF09C2C1B34B0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2A7</accession>
    <name>A0A5B0R2A7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Vacuolar protein sorting-associated protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">VPS5_2</name>
        <name evidence="14" type="ORF">PGT21_013741</name>
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    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="271164at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="GOC"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00787">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
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    </dbReference>
    <dbReference type="SMART" id="SM00312">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="575"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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    </feature>
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        <location>
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        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
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            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
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            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="369"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="486"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="957"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1015"/>
            <end position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1119054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1087" mass="118753" checksum="9B1A8420B5591AB6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2X3</accession>
    <name>A0A5B0R2X3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2X3"/>
    <dbReference type="OrthoDB" id="2508355at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="353" mass="40812" checksum="CC7F013114C4D1A1" modified="2019-11-13" version="1">MKLFQRLVDHDYNLRLNVTASDLRTQNMLSKGDLFTELHSTLLPLLRSHISSLSVALRNSASLRINQAPTLKCIIEIQMKLELTLDQTIYILNDILPGQLLAPSQTNDQHFKQFKFYRLRRLESSIKHRMRGKLALFFSNCRQLIEETTTPGRSRTLATFSPAKALQSIDLAIRWSSSELNLIYFIWIDELSKINTLCVRLSAVADPDQQFYNNKLYQSFKPVIKLSRLFFRKITTAGMTNKQAPFYTEMSSYQLNLLEETVGKITDTLTDFVRSFEQDNQRRPESARVIIPRLKNLTTLFQSCLFLTDLYIVPVFADVPSQAYLKTWVVTWNTLFYQATHNATKACESFQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3H0</accession>
    <name>A0A5B0R3H0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_037135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3H0"/>
    <dbReference type="OrthoDB" id="2150628at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1120086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="88" mass="9752" checksum="FEF7ED0B6781C316" modified="2019-11-13" version="1">MVVCKHLVGSPGYLSYLKLSEELGNAVVDEMVARNFLHHRPLSSFSRDLVPTPSEAVLTPQSAPARIAMKTLFSRYVSYQAIAMKEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3T9</accession>
    <name>A0A5B0R3T9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_037234</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3T9"/>
    <dbReference type="OrthoDB" id="1368at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044669">
        <property type="entry name" value="YneE/VCCN1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281:SF21">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281">
        <property type="entry name" value="UPF0187 PROTEIN YNEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25539">
        <property type="entry name" value="Bestrophin_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="223"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1120177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="480" mass="52737" checksum="2B8FF70C3FB8CAD8" modified="2019-11-13" version="1">MSHSNTPKRENTAPLAIPRVLAALKDLIVVEETNEERIKLNNKVAWFHDVFRLRGSTIPHAFKAVIMFTLWSTVVAVADLVYETSLNLSNSVVPLLSVVVGLMLVFRNTTAFARWEDGRKAFTSLQSAVRNTARDYWISIGAPVHTSNPKDPSPASLPPKEIRLKARALRLLVAYVTAVKRHVRGQYGIDYEDLRALLPPEFYNRPSQPGFGFDAADADIKGSSSKALHTESPPPLSGPLGGGSSRSSNNVPSSDAIDGNLAQSTLKRRPAYQRVTSFFFSQREELGLATGDDSVGVSDESSPLLRHRPSDQRMEDSSVVVVHNFLSRPSLPLPLVIAHQLHLYITRCKRKGYLEAIGPAGHSRMVASISTMTDLFSSIDGLGMTKIPACYCIHLKQCTTLYLWALPLTLASDLGWALIPFVSIVAFTLVGIEALARECEDPFGVDPSDLPLDYICADLRNEVEHLIAKLGSDPEQDLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RBT2</accession>
    <name>A0A5B0RBT2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Iso-1-cytochrome c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CYC1_2</name>
        <name evidence="13" type="ORF">PGT21_008743</name>
        <name evidence="14" type="ORF">PGTUg99_019604</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="11">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000210">
        <property type="protein sequence ID" value="KAA1123281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RBT2"/>
    <dbReference type="OrthoDB" id="449280at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000001">
        <property type="entry name" value="Cytochrome c iso-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="CYTOCHROME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00604">
        <property type="entry name" value="CYTCHRMECIAB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 9 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 9 11" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="12">
        <location>
            <begin position="10"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1099482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1123281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="112" mass="12102" checksum="5D51191D39A4DE28" modified="2019-11-13" version="1">MGKKDSGGEGDPAVGGKLFGQRCATCHTVEKGAPHKVGPNLHGLFGRKTGQAAGYNYTEANVKKGITWSEETLMEYLIDPKKYIPGTKMAFAGLKKEKERANIVAYLKDATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RNS6</accession>
    <name>A0A5B0RNS6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prephenate dehydrogenase (NADP(+))</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TYR1_4</name>
        <name evidence="1" type="synonym">TYR1_3</name>
        <name evidence="1" type="ORF">PGT21_035385</name>
        <name evidence="2" type="ORF">PGTUg99_027393</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RNS6"/>
    <dbReference type="OrthoDB" id="5399569at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1106476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1126334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="183" mass="21306" checksum="393167265638981A" modified="2019-11-13" version="1">MVHLRNLEQSLPHKHQSEPQNKPHLKDFFPKDLIPFRCSDDTDKQSVKTILASLRSRYVELSYKGHDVTMANTDQACTHAAFLRYDYGNRLACYWSIPLRFGRYIGGIEVAKINIIHTFHTPRQKERSSVWTKEDVNMSTLRIHSSKWHVYAGLSDHESDCKSTDRPVRAIGFRAIRPNDLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RRD9</accession>
    <name>A0A5B0RRD9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028424</name>
        <name evidence="3" type="ORF">PGTUg99_013504</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000144">
        <property type="protein sequence ID" value="KAA1128127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RRD9"/>
    <dbReference type="OrthoDB" id="2499725at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="446" mass="51188" checksum="9BC568CF08E07F8D" modified="2019-11-13" version="1">MSDSEVSSDGELELSSNYDSDPSSSSSEASSISENGKPKPKSAKIVTTGSVMRLLDKLIEKFKSSMNARGKDSNIGLPQDEVDRKNDRLIDLEVDYLPTFKLELGFLLGYLGLQKDPTKHPMTFLDLTHQGLLDVEKSLDAIIECIEDVAFMPPAAQTDDHHYQGSKHFRTDYLMRKLWRLIETDIPELLSYYEDYISTWKSSNSRPTQYNCSSTRHFGGVIVKKTDYCYQFVDKIIHWCQASDFDILRDEWVLPIPKLDKLLETLTEKAHQDPANTQEVGRPARLARLTIPFVKLARTLITKVSNTQKKELPFSLDDELNTKTLHKLQYAPNSITSDLEHLTRTVLQSVTPIHSLAESEAEIRRIIHERISKSVDNFLTVLALSLIPLDPDSDRHSLESNYQDWFLTWKDMWDDAERRFLDALFDPDQDEDDVDDGRVFEFRFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RS78</accession>
    <name>A0A5B0RS78_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NIK1_4</name>
        <name evidence="15" type="synonym">NIK1_1</name>
        <name evidence="15" type="ORF">PGT21_028997</name>
        <name evidence="16" type="ORF">PGTUg99_018051</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="16 18">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000144">
        <property type="protein sequence ID" value="KAA1128162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10266508at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000003">
        <property type="entry name" value="Atypical/HisK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000235">
        <property type="entry name" value="Probable nik-1 protein (Os-1p protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1530">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18947">
        <property type="entry name" value="HAMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="435"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="528"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="620"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="712"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="804"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="896"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="988"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="12">
        <location>
            <begin position="1062"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="1432"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1562"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1578"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="1481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1100079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1128162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1596" mass="173134" checksum="6163AD598AF2C2C5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RY26</accession>
    <name>A0A5B0RY26_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PARP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_018872</name>
        <name evidence="9" type="ORF">PGTUg99_017209</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000110">
        <property type="protein sequence ID" value="KAA1130332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RY26"/>
    <dbReference type="OrthoDB" id="429950at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.10">
        <property type="entry name" value="Zinc finger, PARP-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036957">
        <property type="entry name" value="Znf_PARP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00645">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01336">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50064">
        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PARP-type" evidence="7">
        <location>
            <begin position="4"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="202"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1066030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1130332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="349" mass="39236" checksum="56DAC5FFAA0BF772" modified="2019-11-13" version="1">MYQYRVEYSSRKVACTGCKLGPGQSISTGQIRFGRKEIQGSYHHSNWSHWGCLSPQMLSNAMKKLEGKIEGLHGLADLKEDDQMKVKNALKYGHIDPSDVTPKAVQKNKTANADVSGAIATVQEDDQNKITTHRRVLPRRVKPAMGGKEKDDRHKSKRQKRALTTDSNDESANENRHRSEASPSSLEREIRSDAVDELDELAGAKQEKSKKSSQQGEKTGDKHKTKRKEAVISIDSDDESADGEPKSTSNTKSNNDKWKIVLSPLPEIKNWRNERQRRAEKTINSVTDILKTLENSTATTMQSTSSCISPFKEAQSLFHENREKVRIAHKVAQSEIKELLKNSVQDLMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1M1</accession>
    <name>A0A5B0S1M1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ATG9_2</name>
        <name evidence="21" type="synonym">ATG9_1</name>
        <name evidence="21" type="ORF">PGT21_018499</name>
        <name evidence="22" type="ORF">PGTUg99_034181</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">21-0</strain>
            <strain evidence="22 24">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(out)</text>
            <dbReference type="Rhea" id="RHEA:67920"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 19">Preautophagosomal structure membrane</location>
            <topology evidence="3 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19">Belongs to the ATG9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1M1"/>
    <dbReference type="OrthoDB" id="2020634at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LID2</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        </lineage>
    </organism>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Visser B."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <end position="48"/>
        </location>
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            <end position="42"/>
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            <dbReference type="EMBL" id="KAA1064101.1"/>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJ42</accession>
    <name>A0A5B0LJ42_PUCGR</name>
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        <recommendedName>
            <fullName evidence="4">trehalose-phosphatase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_009776</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="EC" id="3.1.3.12"/>
        </reaction>
    </comment>
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        <text evidence="2">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.12" evidence="4"/>
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        <property type="protein sequence ID" value="KAA1064622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="UniPathway" id="UPA00299"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:trehalose biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="HAD-like_sf"/>
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        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
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        <property type="entry name" value="HAD_sf"/>
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    <dbReference type="InterPro" id="IPR044651">
        <property type="entry name" value="OTSB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768:SF3">
        <property type="entry name" value="TREHALOSE 6-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023002902" description="trehalose-phosphatase" evidence="6">
        <location>
            <begin position="19"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1064622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="390" mass="44297" checksum="A8D7ADBB4141B780" modified="2019-11-13" version="1">MLYTIIAIALLFAHPCQSMIPVAHETASSAHAGASAEALGSKAVPLGLERQPIYKVPELRSKDLKGMRKDYMNSEKSYFFIDNDGTIKPPEAEAENTAAYKERVREVLTKLTANGKNQVWLVTAREVSALEKEYGNIPGLNLAGKKGVELRPTNPTKLKLPDVSQIRNKLLQEASAISNRLEIKGDMYSEGPYRLRYMQRHDMAKDKSTEMTEMKKQVAEFIDKDEQFKDFEAIFIEHANQYEVSIQHKTYGTKALIPTLMLEKESPKFALSMGDMDMDEKMHEVMRAAGHRAIIVKKPKSSWQRFLAKYREPEPFDFKAGPAFNSFATHRLDNHQKAIRFLESLVETPKTFSQKFASLWKNWEGFLPSLLRKIHSKLGSGKKTENSLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKS4</accession>
    <name>A0A5B0LKS4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKS4"/>
    <dbReference type="OrthoDB" id="10268791at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="156" mass="17705" checksum="E56181440BA76837" modified="2019-11-13" version="1">MFSANWIQRSHYFLYAVIAMTLLPSVQLLESHPHSIVRRYRGNEPIKTCSLLFTLNGPDGHSRCQTADKSVYSCDFRDCPHFNVPNGFSLRECRAIQTPTAKPFGKAKEVQPFFYKDEGSSIEARDASDTYYECSSQANKYRRLSCNSCLLVSKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LM23</accession>
    <name>A0A5B0LM23_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015169</name>
        <name evidence="1" type="ORF">PGTUg99_018896</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LM23"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="68" mass="7337" checksum="D4CA0252FE93755D" modified="2019-11-13" version="1">MPSQQISKGQPSCGFPSNCITVAPRGARLTPHRAIGYKPPAVSSSSRWVGPSRSVIQQVKSGRTYRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNR7</accession>
    <name>A0A5B0LNR7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Origin recognition complex subunit 5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023978</name>
        <name evidence="4" type="ORF">PGTUg99_009797</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNR7"/>
    <dbReference type="OrthoDB" id="365981at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020796">
        <property type="entry name" value="ORC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047088">
        <property type="entry name" value="ORC5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14630">
        <property type="entry name" value="ORC5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 5 C-terminal" evidence="2">
        <location>
            <begin position="480"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1066172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1072649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="639" mass="71942" checksum="BA4BD64187794FE9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LP93</accession>
    <name>A0A5B0LP93_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024894</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LP93"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="11960" checksum="80492D2C25C30792" modified="2019-11-13" version="1">MATKFSNMIEENPRFFKNDSNESKEISERLLKRTEELKALFYQFNKAWGSSCAWLSEQTDIEQTVHNMAQQLAGFKAVFKFFFGNDHFSPSLFDYQLDHFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQU9</accession>
    <name>A0A5B0LQU9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription initiation factor TFIID subunit 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TAF5_1</name>
        <name evidence="10" type="ORF">PGT21_031346</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQU9"/>
    <dbReference type="OrthoDB" id="10266330at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04494">
        <property type="entry name" value="TFIID_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160897">
        <property type="entry name" value="Taf5 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="9">
        <location>
            <begin position="109"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="490"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="538"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="580"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="622"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="664"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="302"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1066476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="787" mass="86920" checksum="6B45371E1A0E9563" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRH4</accession>
    <name>A0A5B0LRH4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010908</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRH4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022951396" evidence="2">
        <location>
            <begin position="27"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="546" mass="63509" checksum="10529A1B4384B8F1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSF8</accession>
    <name>A0A5B0LSF8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-arabinokinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022261</name>
        <name evidence="3" type="ORF">PGTUg99_001132</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000009">
        <property type="protein sequence ID" value="KAA1137304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSF8"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053205">
        <property type="entry name" value="GHMP_kinase_L-arabinokinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38134:SF2">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38134">
        <property type="entry name" value="SLR1395 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="53536" checksum="C86B2ADEA5E29ED1" modified="2019-11-13" version="1">MTTDGPLYHFAYYCSGHGFGHATRVTAITAGLLSRRHRVSIVSSAPESVFEAVLHGSSAQASYRFAIFEPTVVQPKAYDVDRLATFNNLHSFLTQQRHPVIDQERQWLTDHRVDCVLIDAPFLPCTAARKANIPSVIISNFTFDSCYSYLSAFNEEASGEQRIETKRLNELVAITVQDYAQAELLLRLPGAIPIPGFDEDIPLPATTWVEDRTGFFTAEIDGKLAKGKDSRMRPRRTIDMPLIVRPLTRSAYEPKTKLALLAALGVPAELLKSETKILLVSFGGQVIPSPSPPTSTDHDLDDGSSPNQTRPHDSLLPPGWIAIICGLPPKARPHQIPDRFFSITDSKFNYVPDLTAIADVVLGKLGYGTCSEVIATRTPFISVPRKMFVEEYGLKRLMKESSTINVEMSIKEFEDGRWASYILRADRLKQQQVHKDHPISSNHHQQHPQLIVNESQNGHAVDIVVDHLEVFMFEKKTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVN2</accession>
    <name>A0A5B0LVN2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016576</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVN2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="146" mass="17471" checksum="C4D0664FC47FE099" modified="2019-11-13" version="1">MSWRTGEIGRERILHMTVTNLEEILNGEKLDLEESWRRYMIDHRFTEKEFNLHDAPQAENLRDTIYNWCLGENKAWSTLRSTDIEAWRLSKLKELKNFKFTKDTVWVAVDFHTFTMAWNSSFTYDAFAYALVISRKWGNMIEIHAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVP4</accession>
    <name>A0A5B0LVP4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024167</name>
        <name evidence="2" type="ORF">PGTUg99_024908</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVP4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="105" mass="11503" checksum="F3BE08198E7B280A" modified="2019-11-13" version="1">MTASPSQPQESQDLLLLEIFLKSNLSKQNTTLFFSATSTAEVAEETLDGLSDLESDSDDLDNKPAKKQKQKLAQPNQPSTPSKEEAYASKKKPEEPKPSKPKPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXU1</accession>
    <name>A0A5B0LXU1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL49</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_003644</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXU1"/>
    <dbReference type="OrthoDB" id="19439at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007740">
        <property type="entry name" value="Ribosomal_mL49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477">
        <property type="entry name" value="MITOCHONDRIAL 39S RIBOSOMAL PROTEIN L49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05046">
        <property type="entry name" value="Img2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1068849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="177" mass="20259" checksum="EA9522AF9D73529F" modified="2019-11-13" version="1">MASTGLRTLYRLASKPLALRPVLCSYRGLPVSSGERRWSTSEAQEKPIGTDPLNPSKEFRKLTNHSNDPHSSNETYKFNIPDRVWKAPVATEDKPLKYTIGRTTPYNTLPVYSVMKPGLGQIWTLIKRIDGDLYKLKEDLILDFPESQPILKLHVKQVLCRGHITKEVKLWLQDRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXZ3</accession>
    <name>A0A5B0LXZ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_032430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXZ3"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14836">
        <property type="entry name" value="AP2_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043532">
        <property type="entry name" value="AP2_Mu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="10">
        <location>
            <begin position="169"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1069737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="432" mass="48956" checksum="E414A6442FE9F4D9" modified="2019-11-13" version="1">MISALFILNQKGEVLISKLFRPDLKRSIADIFRIHVISNPDVRSPIITLGSTSFFHVRHQNLYLTAVTKTNANAAIVFELLYRIINIAKSYFGKVDEEAIKNNFVMIYELLDEILDFGYPQNSEIDTLKMYITTESIKSEQAVREDSAKITIQATGATSWRRNDVKYRKNEAFVDVVETVNLIMSSKGTVLRADVDGQILMRAYLSGTPECKFGLNDKLIIEKTDRAKPSGSTRTDESAVELDDCQFHQCVKLGKFDSDRTISFIPPDGEFELMRYRSTTNVQLPFRVHPIVEEIGKSRVEFTVHLKANFDSKLNANSVVVKIPTPLNTTKVACKAQIGKAKYVPEENVIIWKIPRMQGQSDATITASADLSATTHRKAWSRPPINIDFQVLMYTSSGLLVRFLKVFEKANYNSVKWVRYLTKASGSYQIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYR1</accession>
    <name>A0A5B0LYR1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Dyp-type peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_050362</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DyP-type peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYR1"/>
    <dbReference type="OrthoDB" id="3207336at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049509">
        <property type="entry name" value="DyP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048328">
        <property type="entry name" value="Dyp_perox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006314">
        <property type="entry name" value="Dyp_peroxidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01413">
        <property type="entry name" value="Dyp_perox_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521:SF4">
        <property type="entry name" value="DEFERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521">
        <property type="entry name" value="DEFERROCHELATASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21105">
        <property type="entry name" value="DyP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20628">
        <property type="entry name" value="Dyp_perox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51404">
        <property type="entry name" value="DYP_PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022791824" description="Dyp-type peroxidase" evidence="10">
        <location>
            <begin position="28"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="DyP dimeric alpha+beta barrel" evidence="12">
        <location>
            <begin position="38"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Dyp-type peroxidase C-terminal" evidence="11">
        <location>
            <begin position="348"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1069003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="57061" checksum="19C7412DB4F971EC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0D6</accession>
    <name>A0A5B0M0D6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0D6"/>
    <dbReference type="OrthoDB" id="2497884at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027850">
        <property type="entry name" value="DUF4504"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31366">
        <property type="entry name" value="UPF0739 PROTEIN C1ORF74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31366:SF2">
        <property type="entry name" value="UPF0739 PROTEIN C1ORF74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14953">
        <property type="entry name" value="DUF4504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="266" mass="29495" checksum="39CFE9F4D592E1A9" modified="2019-11-13" version="1">MEPLLALHDLISQLIIPHRRHASTTLINRLVADIWAVQLELRSACLLDGIAISEEQAVQIADGLKNITCLVNLVVLHQRLSSSTFICSRSLWKARYDPAWWKPICIDLSSTPPQILDHIPQPVMCALKTCETSGQLQTSNRLVVELQPASTLVSLAGLFLGYPCTYCNCSTNQIVAGPLKVVQVDLLQLNPSAEKIPGILPSRHQLMAFTFPAAFDYPGSPLELEDLIKRLKHQIEQKLRSAGCSYGWEGVEINVEQTVDIERVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2G6</accession>
    <name>A0A5B0M2G6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015271</name>
        <name evidence="3" type="ORF">PGTUg99_025295</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2G6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137121" description="Secreted protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="13760" checksum="08EB070728D5280F" modified="2019-11-13" version="1">MHLSKPWTQATLVPTALVAILGFQLSQAMTPCEKIRLGIKIGKHIPLEANVNSKELIAIMEEAVSKNPDLMHNTDQFSARLKEECAKLGDSSFPACGHGQTSIGKGNKVKKFCLKCLSVAINPGNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3Q0</accession>
    <name>A0A5B0M3Q0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Splicing factor 3B subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_019648</name>
        <name evidence="14" type="ORF">PGTUg99_010670</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000174">
        <property type="protein sequence ID" value="KAA1125863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3Q0"/>
    <dbReference type="OrthoDB" id="10259687at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12335">
        <property type="entry name" value="RRM2_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000059">
        <property type="entry name" value="splicing factor 3B subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000121">
        <property type="entry name" value="Splicing factor 3b subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034159">
        <property type="entry name" value="SF3B4_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="13"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="100"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="205"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="338"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1071797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1125863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="392" mass="41646" checksum="283451B078AEA480" modified="2019-11-13" version="1">MSRPQDGDRNQEATVYMGNLDERCTDALVWELMLQAGPVVNVHLPKDRVSMSHQGYGFCEFLTEDDAEYACKIMNQIKLYGKPIRVNKASSDRKQVDIGANLFIGNLDANVDERMLYDTFSTFGTLVQTAKIARNPTTGQSNGYGFVAYESFEAADTAIESMNGQFLMNKAITVQYAFKKDGKGERHGTPAERLLAAQARKNNALPSATGANLPAPPPSAPNPMRYNAANAQSTPSSHPSNNPASNGPPGSFPPSGGGGHHPHQPYHAGGGQGGGHHNPAMSYHKPPGPAPHGSYPGPPPTTVPMAYRPAQTNSFQQPPARNFPPPPAGFAPTNGHSPFPPAQIPPPPTNFAPPISNGTGPHSFPPPSQNQPSGLPQPNQGIPLPPPGFAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5Z1</accession>
    <name>A0A5B0M5Z1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5Z1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="370"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="406" mass="44660" checksum="D4AAE07052A1904A" modified="2019-11-13" version="1">MLIPYKKTKVYIVDNLLTSFSFFLSFSSRLPLIVPNFSLCLYVCFLCWLCTSIPLCVYVLPILVGHCLYVCTTIAFSKIVSIASSSSQSSAYDLQYITPYYLEEDEPEEEEELTQQTIPEDPARRSNGQEQDYQTANVGRSGASLANTEEPGSIEEPDDRLTDQVLIQIAARSLLCLDSLNAIDGETDRATNLVKENGRGSSSSRESRPSTRASETSFRMSSSTSTSSPEDSRRSSVPSSSLYDDPDSLVNMSEHSNTSLDSRIRSPDSSPLHHRRRADSVRRRGSSGSSSRSSASSFVELFLSSSGEAHDDDPVNLHRNLPSAVLHTRNHPSLKRLARKTWTSPAVQSAWKRLGSHSTLLHPPSVKLPSSSSSSSSSNTTTTWTAGTTNSSNFFSSLFSRKLHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7A4</accession>
    <name>A0A5B0M7A4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030656</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7A4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="61" mass="6842" checksum="4F9DA7F6D232B268" modified="2019-11-13" version="1">MRLGFVSQYLANDRQIKAHGHSHITGEKSFYAGSEKHGTVNIFKNFSVSAKKDTRFSLARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9Q7</accession>
    <name>A0A5B0M9Q7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial</fullName>
            <ecNumber evidence="6">1.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Isocitric dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NAD(+)-specific ICDH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">IDH2_4</name>
        <name evidence="18" type="synonym">IDH2_6</name>
        <name evidence="18" type="ORF">PGT21_033906</name>
        <name evidence="17" type="ORF">PGTUg99_026154</name>
    </gene>
    <organism evidence="17 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
            <strain evidence="17 20">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-threo-isocitrate + NAD(+) = 2-oxoglutarate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:23632"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Octamer of two non-identical subunits IDH1 and IDH2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.41" evidence="6"/>
    <dbReference type="EMBL" id="VDEP01000476">
        <property type="protein sequence ID" value="KAA1072903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9Q7"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000003">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
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        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="64"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013012"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030683"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00072026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1072903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1075335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="393" mass="42666" checksum="C4335262034718F2" modified="2019-11-13" version="1">MITLTRGLRATSRSISRYQGQAKTFGSSISSQVAASPAGTSGKKPSSAETGAFKGFKLPDQTYKVTLIPGDGIGPEISESVKAIYSAAKVPITWEERDVTPVIVDGRSTIPQDAIDSIHQTTVALKGPLATPIGKGHVSLNLTLRRTFKLFANVRPCRSIVGFKTPYDDVNSVLIRENTEGEYSGIEHEVVDGVVQSIKLITMDASERVARYAFQYARLNKRPHVTAVHKASIMKMSDGMFLQACRRVKEDFPDIKYDEDILDRVCLRIVQNPAPYSDRVMVMPNLYGDILSDMCAGLIGGLGLTPSGNIGRDASIFEAVHGSAPDIAGQGKANPTALLLSSLMMLRHMNLPQYADRIEKATLETIAEGKHITGDLGGKASTQQYTEAILRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB42</accession>
    <name>A0A5B0MB42_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013507</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB42"/>
    <dbReference type="OrthoDB" id="2507780at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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            <begin position="23"/>
            <end position="830"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <end position="322"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="763"/>
        </location>
    </feature>
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        <location>
            <begin position="776"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="830" mass="80466" checksum="A94D6D1023F0C605" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MC06</accession>
    <name>A0A5B0MC06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MC06"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="280" mass="31311" checksum="033820DD3E92751D" modified="2019-11-13" version="1">MTEADKAADHQVQQLHHPRPQPFLPPPRLPLRPSSSATHSKPALDKPMRTVLENLDRNFHKTHTTRLSPIISSDYPLVLLPPTIPQPNLTSHPSFPIMEQSTDMENTLVGGGSSIFMSTALMLMFESIQQISPSSLLDQSLSLDQSSMLMDQSSLMLMDQSDNSLQLGLSNVNLGCSSDPDQANRQANNSTELGKLPPPPAPKLCRRTQDKMELFCAEQEQAHLCRKQERDMAKLIKNQQKEAQKQAREAEKQPKEADKRRKTQSGLRLTRLSCSEDADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MC08</accession>
    <name>A0A5B0MC08_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bud emergence protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_010789</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MC08"/>
    <dbReference type="OMA" id="EYWFRID"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06890">
        <property type="entry name" value="PX_Bem1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000050">
        <property type="entry name" value="Related to Protein scd2/ral3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035550">
        <property type="entry name" value="Bem1/Scd2_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF2">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="61"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="192"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="6">
        <location>
            <begin position="432"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="129"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="294"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="575"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="342"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="576"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="607"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="644"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="667"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1073394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="796" mass="88119" checksum="1A39359A3798DEBB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MC27</accession>
    <name>A0A5B0MC27_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MC27"/>
    <dbReference type="OrthoDB" id="2495569at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="1">
        <location>
            <begin position="13"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="374" mass="43130" checksum="2945474A96BD8617" modified="2019-11-13" version="1">MYKYVARPSHPKGKLPLDPPDVKLISHKRPEIHMLKLMNKSDNSAEGIGQVMNALQHQTRLDPGDFFGRLQLIDGDLGTTQIFNAIRVLRSPSEHCEHSLNNISFTLGAAHTLWNIAHTILTYHFGNSSSMDDLGVWRYLQALGVPPEKVVQKKDFTKMLQYMEQVHEATLWYCLRDDHDTVIQEELPVIQTSKWNDTVDKCYDQFFSHEARRAARGSPKLSNLLTRLQDFSTVIEANRAMKRGDIRRLINVWKMWSFMTQSLPGLTHYSAYLPRLILLLTKILPPSLGKLIRHSILVSPSGRPNHFVAKYFLLETHNYWLKYFYTRRGIGTQIDRLQTLFSSNIPTLRTMFDSLRLDSGAKHVQQSHKPLLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCM4</accession>
    <name>A0A5B0MCM4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023452</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCM4"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="52" mass="6050" checksum="05D7EB58247C2195" modified="2019-11-13" version="1">MKDPGHRTYEYLPAGGFQANLDDELDYLGLVPKIKVREVMDTLKSPLCYRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEC2</accession>
    <name>A0A5B0MEC2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEC2"/>
    <dbReference type="OrthoDB" id="2507429at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8790" checksum="68DD3EB3F37DF5C9" modified="2019-11-13" version="1">MDNERIKDENDSIDPDLDPTATKKKKAPNYTKPEDFELCRAWICISEDPAVGTHQDRNTFWQRVTTAYHEAIPTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFQ2</accession>
    <name>A0A5B0MFQ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFQ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="140" mass="15933" checksum="39F9971B6D956D0B" modified="2019-11-13" version="1">MKVGRVDLEVTRDPSGLGVGSGLYRIALQERCGAPHRIACIGFFDTVYQYRIGKAMHHNLVASVCIAFQYDADAMQCIAFWMQYRYMAIYRIALHRFTVDLTQPECDPLDYRVGFRQQVLISGRPKNPTTDPLKGSQGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIW3</accession>
    <name>A0A5B0MIW3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_037144</name>
        <name evidence="2" type="ORF">PGTUg99_004643</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000456">
        <property type="protein sequence ID" value="KAA1077117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIW3"/>
    <dbReference type="OrthoDB" id="2135488at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052350">
        <property type="entry name" value="Metallo-dep_Lactonases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43569">
        <property type="entry name" value="AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43569:SF2">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="49239" checksum="5F24FAFBA43BB138" modified="2019-11-13" version="1">MPKKPPPHLPLQAFSSNPASSEQQANQLLTRRVPLVAIDAHVFLPEDSEAGHSRTSAKLWHDSIQPFYPEQITSAGDHQQAHSHQTTTQFKGFIHIHQQPAWLLEQTPDDPQQAAQLSSKRWETESQNILRIDKELVEIQMAPPLGQVIWAPLHQGPSVLGSYLDQLSNLSIRICGCCHLIERKSTTRNVSLVDPEFIESLKLLGERGLSVELMVDGNHERSARAILDEVLECVSAVRRGQDSKSETKFVLCQMGMPEMISTQSLVPSSASYSSILSRLFSLSLFSDFHIKLSGLPLILEEELNKRACKRFLQSHRPNPRRDLEPSTPVAVHEDEDGGKQETDRDALAQGESAWREVKKRIKFYLEPILEAFGDHRLIYASGFPYYDVPQVSSPADKATGRYEYQFEIFRECLTEFGLENGALDNVFGLNAQRCYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLC7</accession>
    <name>A0A5B0MLC7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036825</name>
        <name evidence="2" type="ORF">PGTUg99_002009</name>
        <name evidence="1" type="ORF">PGTUg99_005095</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000491">
        <property type="protein sequence ID" value="KAA1069976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000461">
        <property type="protein sequence ID" value="KAA1077083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLC7"/>
    <dbReference type="OrthoDB" id="10579682at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="80" mass="9600" checksum="FF00DBDBFAAA1B5B" modified="2019-11-13" version="1">MGRSEVRQRKKRATSSQFSKCLVLNKQRKSLTLYLIPNHLWWYNPSEATYFGCLLLLGINAGRRRLMLYFATEQFRWRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNE9</accession>
    <name>A0A5B0MNE9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNE9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="618" mass="68067" checksum="06C7AC4281CB1B02" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPN1</accession>
    <name>A0A5B0MPN1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPN1"/>
    <dbReference type="OrthoDB" id="2507296at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="285" mass="32657" checksum="1F58D8BC07A6B2C4" modified="2019-11-13" version="1">MSDSTSRPPNQPFHPPPIDYDRFKNDRRISRPPGVPTLWSRPSNQTDQTPPPPQPSASKIPDILPTRPRSSDDKKSKDVTKKLHPPYSQVKAQAVVKPSAPAMKNTSQPPKEVQHRTVVNLSKREKPPKDISTSSKAKIETSKDDRKPHLSQNNPSSNTKIKDNRQEIKTMPIEELESRHRHALRKLQQRLEENQAGASIDKVRLRKQYEQEIDQLRARMKHNEISTRPRTEQSRLNSTIPDKQGGLKPAQKVPQEDSSQTIDSRNPDLPPNFSNQSRRQSWLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MS73</accession>
    <name>A0A5B0MS73_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MS73"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="176" mass="19648" checksum="EE48767F2062F981" modified="2019-11-13" version="1">MMTNSPKSTYFSFPSYESHFISEVEEAPTPVTATLSQKALEPMIDCSAEAYPIIQRGSHLEAEIELILYLNAPRKPASLFSESSSSNKRRPDKLIINDKSSNSQTSTVHKSSQGTRPYMPGSSEWSSQQASPKDQSLFFQPSNTLSSRPRVQKLDHSFKTQEQKQKGSHFGKSQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MS81</accession>
    <name>A0A5B0MS81_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptide hydrolase</fullName>
            <ecNumber evidence="9">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LAP1_2</name>
        <name evidence="11" type="ORF">PGT21_025373</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M28 family. M28E subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="9"/>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MS81"/>
    <dbReference type="OrthoDB" id="2214at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000042">
        <property type="entry name" value="Peptide hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF56">
        <property type="entry name" value="AMINOPEPTIDASE YDR415C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023068115" description="Peptide hydrolase" evidence="9">
        <location>
            <begin position="26"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="10">
        <location>
            <begin position="253"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1079827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="461" mass="51475" checksum="646892264332F73C" modified="2019-11-13" version="1">MLNYSTIIGAVALLLLSTATQPSAAEEPPLVINRATNQINFQLSPPIESLLGGENQHRLSQEKTESLLTDNHLERITEHIKAYPEKRLIRWADGRQEWTTEGEKALLIFKDGRRKFVDITDSWLASDLHSDAFTLDSDPQLLNPTFVTHHSSDLLHQSNSPVHSFPHKIKLGHQAVANFTRLIQPARMKKRLTKFTDFRTRYYRSSTGKESQAWLLNEIRTLVSQSKSNVTVSEFPHKWGQNTIIARFEPNGEEAATRSSGTFIVGAHQDSTNLLPFLPAPGADDDGSGTTSILEAFDILLHHNWTPKAGQGALEFMWFSAEEGGLLGSQEVARAYRQANRPVDGMVQFDMTAFVKKGTTPTVGLVTDFVDPSLTQYLRLLIDEYLAIGWTNTKCGYACSDHASWININVPSAFAIESSFEDSNQKIHSTGDTIHQPEFSFDHMAEFSKLVLGLVLELKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSY4</accession>
    <name>A0A5B0MSY4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_002944</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSY4"/>
    <dbReference type="OrthoDB" id="39175at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050815">
        <property type="entry name" value="TF_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338:SF29">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="95"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="826"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="850"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="867"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1079184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="931" mass="105427" checksum="576D65448278D886" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MT55</accession>
    <name>A0A5B0MT55_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001931</name>
        <name evidence="1" type="ORF">PGTUg99_009823</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MT55"/>
    <dbReference type="OrthoDB" id="2504757at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="242" mass="28553" checksum="42EFDB5324B15235" modified="2019-11-13" version="1">MNYILACPYKPEWWRSVGLESSIKEFGLDLLIILKIGNSLQFGSMYFVENSESTIPLEKAYSTIIDIMISKRNFPWIESPERAWLYRFCGSFYQDRLRELSHLVFSKHVTIQDEMKGPSGFELIWCDLGLNIELMPHIFELGENLNIAEELKKYEKIYGVFSEYAKMAISVWVRSFTSKPQKYLGNMKKSQAIPLWEDKEIKKRNKEFYPTEMNWSRSLTNICNLMSLEKMEKIKRLHGIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MW48</accession>
    <name>A0A5B0MW48_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_000906</name>
        <name evidence="10" type="ORF">PGTUg99_004309</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000388">
        <property type="protein sequence ID" value="KAA1090347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MW48"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000192">
        <property type="entry name" value="Vacuolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052706">
        <property type="entry name" value="Membrane-Transporter-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310:SF4">
        <property type="entry name" value="AFR304WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310">
        <property type="entry name" value="SULFATE TRANSPORTER YBAR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="537"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="590"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="8">
        <location>
            <begin position="661"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="7">
        <location>
            <begin position="861"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="780"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="797"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="807"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1080278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1090347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1010" mass="111382" checksum="B526F3CBEB76D0A7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWI5</accession>
    <name>A0A5B0MWI5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWI5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10528" checksum="8E461BBD4F7AF45E" modified="2019-11-13" version="1" fragment="single">MNTARIEAPASKGSSTGVELQLEGNSQDLNRASSNLIAQILYQVVMIVYRASLPYLQASMTRCQAFTDEPIFRSYRPPSPPRASLPSAPFVFYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXB4</accession>
    <name>A0A5B0MXB4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXB4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="92" mass="10198" checksum="7CE3ECB268F410BB" modified="2019-11-13" version="1">MAAYCLSLQQVNFEPVRDLIWIRVDGSEKPQSSQGAGSIRGGVPIDLDVESLNLRLERSSAQPAIIRASHRPTFIALDIRPRNQSPAEEETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MY31</accession>
    <name>A0A5B0MY31_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Eukaryotic elongation factor-2 kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">EEF2K_13</name>
        <name evidence="8" type="synonym">EEF2K_6</name>
        <name evidence="7" type="ORF">PGT21_021060</name>
        <name evidence="8" type="ORF">PGTUg99_011559</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000047">
        <property type="protein sequence ID" value="KAA1134671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MY31"/>
    <dbReference type="OrthoDB" id="2505915at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903013">
        <property type="term" value="P:response to differentiation-inducing factor 1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF2">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1080789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1134671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="255" mass="28090" checksum="1A10329C9C627A5E" modified="2019-11-13" version="1">MYKASGHLLQAYQSVIKRCPSKLLSVTLREKAKAFKLVHHCVIYIGEEICPAEVYFLEACLEGDYVKYLSNVNFSITDRQPGMDINNLSIMNAFTHWSYFISNGASLVCDLQGVGSTITDPQIIDCDYACWADGNNASKGINHFLENHVCNDVCRALKLGSPTDITPEVTEPDRSVQSQVARATIQAAQSLTGPHAMTDLRTFPGLPANPAQSRAAIGNLLRTEVHGETYPNMYFFASELNHQTSERGPVNTSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6Z2</accession>
    <name>A0A5B0N6Z2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_030340</name>
        <name evidence="6" type="ORF">PGTUg99_024041</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000342">
        <property type="protein sequence ID" value="KAA1099236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6Z2"/>
    <dbReference type="OrthoDB" id="6159137at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF35">
        <property type="entry name" value="RNA ANNEALING PROTEIN YRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="246"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="163"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="429"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="465"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1084532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1099236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="491" mass="53474" checksum="9CB8E8F8D3FDF1B1" modified="2019-11-13" version="1">MSLLPDRPRQSLFPSRSLHGRLAPADHHPRFGSNPSLRSKPYDKPLTAPMRTAPDSRWSHDLFEDDSNLYGPKVRYQMKGSDQISFGEAPRPSPSLRPFGNSVPTPASLPVSSLAKPISSTPAPLPIQPIVSQHLPTSIKKPQPVPIRSNSGISLLSRIQSSDTHKPLLGNDPSSSAILLRPSNNSTTNTPPNFWKLSDPTLINRPDPSIHHSHPPPQEIRIQQSQAAKNHQAFDEAMAKYLNGPVILEVANLADGTSAEDVKTAFADFGEIQECSTEEGLRQANQPTLKARMIFTHKAEAEKAVEALNGALADGLTLAVKIVGRPTKKPERADFMTPSSPVDFKTLKSAADAAKKAEENHDVAMEDGDTGIVPTGCLRSEAVAQCDPRASIQVEPRPPAIYQLEHPKPAQSIKPNNLLMNRVQPLARPTSQPLLSSSSNRHHPAQSLFSAPRNSAFGKPLHSSHLNHSHNRNHQIHHSGSKSLLARIKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAS7</accession>
    <name>A0A5B0NAS7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020756</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NAS7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="89" mass="9725" checksum="AB989C8DBA29C6CC" modified="2019-11-13" version="1">MAVSKSIEDGFSTALGSTHKKVNDIMYLGIQAMQADGMNEGMILKRFNPSTNLLDVTGLYGSNYAGDQAIETEILDPDNELHTHGNMEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NC08</accession>
    <name>A0A5B0NC08_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MutS protein msh5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MSH5_3</name>
        <name evidence="2" type="ORF">PGT21_011773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NC08"/>
    <dbReference type="OrthoDB" id="29596at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="279" mass="30644" checksum="ABBFCB8CC55DEC78" modified="2019-11-13" version="1">MTSFGQEVWWLYAALYKKDLQVQLEALEKAGVRKLQQVTLEVPIVGAAPVMEKALNKHFAFAVNGWKNTMGGAYYDPSNGIFYLMEDAVDTGGEFEVLIMLLEQVMPEVVLLSSKAEESLTSCIQKAECPGGIDKTPTVVKRPSREYSASDGFKKLQRLSVRSQDGVVPSGNHHSSSTSTRSTASQDPGRDIHFSKNSNLTANAFINVEASHYSVGTFPQVKPLEGLRDEFNERILFCQLGAVAALLSYLQSGIARDQDNDSPQGGLFVSKVEMIKTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEG8</accession>
    <name>A0A5B0NEG8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Syntrophin, gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEG8"/>
    <dbReference type="OrthoDB" id="2507786at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="42427" checksum="5BE0A5B2A8AB268A" modified="2019-11-13" version="1">MSQSDSSALTELDSSDSSALSELEDSIDVPTSTADASSMGSSEQSSAGYPNTRSKTGLSQNNLAAFNNLLQSQADASEKAAPAKPESSCHTASSASRPRFAPPKGPRKQHRTGFRQLPGANGVVKRGRPSKKEYKLAGVEVDMYALKGVTLHGPAKSLDEVKGGQIVARRSINGRRRSSARKPSSTGSAAAIGKSASGPLNAIQEEAPALQQATMVPSQSYRDTQQMLFSSTQGPSVYNYQLGLGGQNFTQQAFPSAFETLNDPNYSMYNTDINGLSQHDFVPPPWDNSLLPDPGFQSLSDIPRLSGLFDQQASLNFAPLNPMQTLVDPTVDPNLNYASLGMPAWTGSDPRLYAYPPVGLDAHNLPLPDSQNFNGSQYSSTGYNQCAYPSGFQNFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGM3</accession>
    <name>A0A5B0NGM3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="14">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">HAS1_2</name>
        <name evidence="20" type="ORF">PGT21_009108</name>
        <name evidence="19" type="ORF">PGTUg99_031285</name>
    </gene>
    <organism evidence="19 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
            <strain evidence="19 22">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="14">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="14"/>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGM3"/>
    <dbReference type="OrthoDB" id="10259640at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17942">
        <property type="entry name" value="DEADc_DDX18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000379">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044773">
        <property type="entry name" value="DDX18/Has1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="138"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="328"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="552"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="583"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="611"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1088501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1105497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="645" mass="72383" checksum="0AB76A5FAFDC1EF6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHE6</accession>
    <name>A0A5B0NHE6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHE6"/>
    <dbReference type="OrthoDB" id="2508336at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022779287" description="Hydrophobin" evidence="1">
        <location>
            <begin position="23"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12950" checksum="24E0D5149B37F01E" modified="2019-11-13" version="1">MQSFNLLVVFAAVLLINTQVNCGSFVCPIPGQPEDQKDAYCIRRITRKELRDPANKNATMFKLHITEKGEDGFTCDDVILNGKPARDSFCCNAPGDIGVVLPSPQSMWDNKCSIIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHU7</accession>
    <name>A0A5B0NHU7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHU7"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022781394" evidence="2">
        <location>
            <begin position="21"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="417" mass="45079" checksum="745F2B03CE32FD75" modified="2019-11-13" version="1">MISIYVSAFVICLFQLGVSAGPPTPSENVLSISVTPIYANSSALLPQSTLVPADGIFRRQTTTPRLLTQNMNNPSTVVSALNNTNNRPANSTGARANDNNRNPKAFTETVRISGGLVRTQASWIDGLKIDAISATPHTLSIKKKTNPAASAKFVTSADPSRSILGGEWRELTSFSYDVSFEGEYPDDLSFKISAPYDKLQAPITVGLDDEVYLGLYDPNRSGWVVDTERMENRRKDKRVDLIGIPAPTGEYRLLARSNRDPLGSMALSFGTGTDGQFNVLAPSGGLSSTPPLQVATWQDGTKIIIRSLQAMQIKMETSNTTTSAIPPGYEAATRYGFTITTNTPAAQVIMNLQLPYVPTQLEARGLFPQDLVTAGRPLRANQQYQILSATTISGNGALMNVPMNIVEGEYLLLARSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJT8</accession>
    <name>A0A5B0NJT8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_007006</name>
        <name evidence="8" type="ORF">PGTUg99_027965</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJT8"/>
    <dbReference type="OrthoDB" id="1806at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF11">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="7">
        <location>
            <begin position="31"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1088440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1105431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="248" mass="28272" checksum="3C5D137CD76FD4EF" modified="2019-11-13" version="1">MPLAKPIRANRRKQHAANARDRVVKLKGDIFQSTTILCEFLYYAFNAILGLREVYPKQDFKWEKRYDIHLPLLIDPDLKSYLTKVTTQLKPWLETKSVTRIVLALVCKETRETVERWQFDIRTTAEQPAGQEAVPQAEPAAADQTPFEPVARSDHDMQAEALQIFRQIFSSVTFMPALEPDRCLFTILSYVDKAAEVPAGWNQSNPYVISATADQVPIHGLSTSVYKLVPTVTYCANQVEQPEEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKJ4</accession>
    <name>A0A5B0NKJ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011684</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKJ4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="57" mass="6380" checksum="E37183008CD0F209" modified="2019-11-13" version="1">MFMMVSGSDSRHNNHLGFNRFSIINFTNTAMRSRASHQLSSAECAVHRKSGNIVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMD4</accession>
    <name>A0A5B0NMD4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">tRNA(M(1)G37)methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TRM5_1</name>
        <name evidence="1" type="synonym">TRM5_3</name>
        <name evidence="2" type="ORF">PGT21_003194</name>
        <name evidence="1" type="ORF">PGTUg99_033189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMD4"/>
    <dbReference type="OrthoDB" id="408788at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="101" mass="11028" checksum="F9A9D12C614A042D" modified="2019-11-13" version="1">MEHDLLRLMIKKDKVVVDVFAMRVAVKRKAWVLGSDLNPAVVEALETNVRLNKLQGRESASAANLEPCIILISSNPTCVSKFGFKATALGEESSSKAFPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPV2</accession>
    <name>A0A5B0NPV2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_030571</name>
        <name evidence="5" type="ORF">PGTUg99_030191</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPV2"/>
    <dbReference type="OrthoDB" id="2506936at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 2" id="KW-0371">Homeobox</keyword>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="4">
        <location>
            <begin position="174"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="1">
        <location>
            <begin position="176"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="147"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="232"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="248"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="356"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1075945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1091297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="389" mass="42656" checksum="EC95F1BF159EA465" modified="2019-11-13" version="1">MIVPKWSTTCTLAVKLRNLAAKSLPSSFLESFNHKKHSDHIPPLHFPEIGNLVPQLLQLGLTADTANLLHREFASTIKTLDETLFESYQTDALKFHSNVDPNPRCSFAEALQEHLLVVRAQSVQRILTTLRTRVDALIQQSRPVELTIQPNPSSPFASTSCASPSSSGPTTTSGKSRIPPQKFTREQHVALNALLARDNQYSTEEKDLIAYELDMTPDQVTRWFCNARARKKPYSCPSRRPGPAGLLQSLSPGSSARDSTMSPSPNPEPTQNFSEEEERPSTSSSSDEDTEMTIIATPSSSSSSSSSSRSDQDQDEPMDAYFDFPAYQSPAQSWPAHSPPSTLNSVPAIPFDFDSLNHSSSSCASSGDPGLWNTSLPQPVPFNFQPFAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPX2</accession>
    <name>A0A5B0NPX2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029942</name>
        <name evidence="2" type="ORF">PGTUg99_028296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPX2"/>
    <dbReference type="OrthoDB" id="2506619at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="287" mass="31108" checksum="ADBBD12CC325A95B" modified="2019-11-13" version="1">MSTTTAYASHLTQPPKTISLEKGAGSETTMTSHGYTAAPKIVCSLPDDSVDASVPGLSSDSDFSSSSSTSSSPKSTRSSFPSLESDDAPIIKSKDNGSLCYDFKALNFDTGRTDFQSLTQNDGQSHLNPQKPHQRDAAPSPIRVTRNQRSFSKTIAVNLTYPDNLAITNQAPKIHMMDTKVNFQVLIGSLAQLLSISPELFDLTIQGNSRTIRLSPIASPHQLGFSGDLTIQVWVRKVATEAHSNLPESLDQPRRIPGTKWIHSFPSKKYKLGLPSSPKFLGNSVTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NT14</accession>
    <name>A0A5B0NT14_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019961</name>
        <name evidence="3" type="ORF">PGTUg99_019936</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000177">
        <property type="protein sequence ID" value="KAA1125564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NT14"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137556" evidence="1">
        <location>
            <begin position="19"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="201" mass="22678" checksum="C25A6AA9FD8E358E" modified="2019-11-13" version="1">MTTMIMLQDLLILNVVFVALDCHWKLFTFVQSMRSPGLPRNNDAKDVFLTEDTKEEIFNASPQRAKAETENTHRQCFRSFSFAQILQAPEWLPLVWVIISAVHFHEIPQAETDLHVTTTHEPLVVNADRLQTSLARPSGPAIRQDLWRQYASLNRRVGSSYTPGRLGRQRGGLLACSPDHHNGVAPAGANFADQPSCRPHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTL4</accession>
    <name>A0A5B0NTL4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RED-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_025521</name>
        <name evidence="6" type="ORF">PGTUg99_006984</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTL4"/>
    <dbReference type="OrthoDB" id="3366823at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039896">
        <property type="entry name" value="Red-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012916">
        <property type="entry name" value="RED_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12765">
        <property type="entry name" value="RED PROTEIN IK FACTOR CYTOKINE IK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07808">
        <property type="entry name" value="RED_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RED-like N-terminal" evidence="4">
        <location>
            <begin position="75"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="232"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="314"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="65"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="499"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1084358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1092253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="539" mass="59615" checksum="2824BFD367462449" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTR4</accession>
    <name>A0A5B0NTR4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Maltose acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CAS91_2</name>
        <name evidence="6" type="ORF">PGT21_019184</name>
        <name evidence="7" type="ORF">PGTUg99_019251</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000378">
        <property type="protein sequence ID" value="KAA1092102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTR4"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03357">
        <property type="entry name" value="LbH_MAT_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF23">
        <property type="entry name" value="ACETYLTRANSFERASE C18B11.09C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="291"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="221"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="494"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="562"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1080747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1092102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="589" mass="64136" checksum="B7BB78CB3E6468BF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NU75</accession>
    <name>A0A5B0NU75_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NU75"/>
    <dbReference type="OrthoDB" id="2507637at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="766" mass="86764" checksum="5C1AC2EF85042E39" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUM1</accession>
    <name>A0A5B0NUM1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUM1"/>
    <dbReference type="OrthoDB" id="2503132at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="107" mass="12086" checksum="A4A125CCC3CF90AB" modified="2019-11-13" version="1">MTTKLSALNIVTGTATCPDPKKDKDNFKLYNKLNEDAYTEIVQYLNQEVLAYVSSALPTTDKFHGYKIWQLLKAKFAGNDLTSKTTALKTFLAVDYDCLTLFLPLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUZ6</accession>
    <name>A0A5B0NUZ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036660</name>
        <name evidence="3" type="ORF">PGTUg99_001641</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000162">
        <property type="protein sequence ID" value="KAA1127573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2245455at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37327">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37327:SF1">
        <property type="entry name" value="MICROTUBULE INTERACTING AND TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="675"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="970"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="441"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="755"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="784"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="810"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="823"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="877"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="919"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1012"/>
            <end position="1042"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1127573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1374" mass="150213" checksum="75074ACE719411C8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NW48</accession>
    <name>A0A5B0NW48_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DBP9_2</name>
        <name evidence="13" type="ORF">PGT21_017252</name>
        <name evidence="14" type="ORF">PGTUg99_031650</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NW48"/>
    <dbReference type="OrthoDB" id="1191041at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF21">
        <property type="entry name" value="DEAD-BOX HELICASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="53"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="268"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="596"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="596"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="638"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1092886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1115777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="669" mass="74061" checksum="E27B0675678183FC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NW77</accession>
    <name>A0A5B0NW77_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">stearoyl-CoA 9-desaturase</fullName>
            <ecNumber evidence="3">1.14.19.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGT21_023415</name>
        <name evidence="21" type="ORF">PGTUg99_019425</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
            <strain evidence="21 23">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.1" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NW77"/>
    <dbReference type="OrthoDB" id="10260134at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000004">
        <property type="entry name" value="Acyl-CoA desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF31">
        <property type="entry name" value="DESATURASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="16" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="19">
        <location>
            <begin position="511"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="140"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1084289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1092320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="638" mass="72683" checksum="523F06001DF7666B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NY11</accession>
    <name>A0A5B0NY11_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015117</name>
        <name evidence="3" type="ORF">PGTUg99_026701</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NY11"/>
    <dbReference type="OrthoDB" id="2507343at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="197" mass="20747" checksum="97EB57086AA091C3" modified="2019-11-13" version="1">MDPRMLLEMYMSMAGARNTAQAAPSEGNQSRRRGRRSSNSRRRAPAPAPAPAPAPAPAATQPAQPVVPNPLLGDGRPQGIKLPFVDSDAKAKAHQDLRSKALCLPTAHPAGARHPRVYTLIGRGNLGGSPQRGTPPRPAESPPEVDPQTDGLPDQVIPAPAPVSQAGPSRRERTQGRTDPYAGSRANRARGPAARDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4P5</accession>
    <name>A0A5B0P4P5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018965</name>
        <name evidence="3" type="ORF">PGTUg99_033747</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4P5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="326" mass="38006" checksum="730977861167643A" modified="2019-11-13" version="1">MRPEGYQCNQLKKFFESLEGDNRYNEQISVKILKAIATLYLLFILNLLIKWANANLLSSINFYFIKNGFLSHQVVAATIYKFWIASLLMKSFADNCEESSSHNSIQSKEINKRAPKKIDWQSGPILLVHPILIRPVILIMFFLLLTVSFFHQLLKLIATAWFDELRTDMTKLLNNNLPPLWFLYSCCCFLFVRPDNRIYADKFTPIEYHSWTKMLADVLKSRRQQSFVFSLAFTLILIGPRFSLMSLKAVNNRHSFSVLHYSPSSRTNTLADVLKGRRSLLSSTGFDLFWHLIHSSFHFDGLDLQSFVEIDRDLARCEKVFKQLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5A6</accession>
    <name>A0A5B0P5A6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5A6"/>
    <dbReference type="OrthoDB" id="5587367at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051614">
        <property type="entry name" value="UPF0045_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00106">
        <property type="entry name" value="MTH1187 family thiamine-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777:SF1">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="MTH1187/YkoF-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-binding protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine-binding protein" evidence="2">
        <location>
            <begin position="79"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="149" mass="16471" checksum="D3BEDCC283DFC1BB" modified="2019-11-13" version="1">MPKVDERSTSMTRCTADFCLIPIGGEDGASVGKYITECQRVLAKTGLEYKVGSVYLFPIDYLISSVEAYRSCMLRFIARSQMHGYGTGLEGPWDEVMNAIKACHEAVHKMGCPRVATDIRIGTRIDKPSSLSQKVESVEKRLAANNFAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5C8</accession>
    <name>A0A5B0P5C8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5C8"/>
    <dbReference type="OrthoDB" id="3189033at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="517" mass="58239" checksum="049D25F122EA1568" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P617</accession>
    <name>A0A5B0P617_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_004226</name>
        <name evidence="6" type="ORF">PGTUg99_002760</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000160">
        <property type="protein sequence ID" value="KAA1127599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P617"/>
    <dbReference type="OrthoDB" id="48651at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001085">
        <property type="entry name" value="Related to TIF3-translation initiation factor eIF-4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF11">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="94"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="152"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="417"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="488"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="596"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="439"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="516"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="571"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="596"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="635"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1096074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1127599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="645" mass="69064" checksum="103C20BB6175ECD0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8J4</accession>
    <name>A0A5B0P8J4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitotic-spindle organizing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_002633</name>
        <name evidence="10" type="ORF">PGT21_002659</name>
        <name evidence="8" type="ORF">PGTUg99_004988</name>
        <name evidence="11" type="ORF">PGTUg99_035384</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for gamma-tubulin complex recruitment to the microtubule organizing center (MTOC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MOZART1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000405">
        <property type="protein sequence ID" value="KAA1088602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8J4"/>
    <dbReference type="OrthoDB" id="48571at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000931">
        <property type="term" value="C:gamma-tubulin ring complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031021">
        <property type="term" value="C:interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033566">
        <property type="term" value="P:gamma-tubulin complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051415">
        <property type="term" value="P:microtubule nucleation by interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022214">
        <property type="entry name" value="MZT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520:SF2">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12554">
        <property type="entry name" value="MOZART1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1088602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1097313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1100956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1134340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="79" mass="8445" checksum="8A17E1F5273B9F9C" modified="2019-11-13" version="1">MNRPTTTKAESKTPRTARDAIDVLHEISELLGTGLDQQTLALCVGMIEEGTNPLALAQVVQELRQEAKGKGKATPAFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA02</accession>
    <name>A0A5B0PA02_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021952</name>
        <name evidence="3" type="ORF">PGTUg99_036832</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA02"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="99" mass="10812" checksum="115405FE9526D079" modified="2019-11-13" version="1">MQRDQTWEKTHPEKGTVCLSIYVCPGLLGHQSFTIPKQDREAVDGNDGDASSGSSGEHGMGAEEDENDGPEDKSDCGMKDKDGEDDQTATLNRNRQENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA26</accession>
    <name>A0A5B0PA26_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007154</name>
        <name evidence="3" type="ORF">PGTUg99_031937</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA26"/>
    <dbReference type="OrthoDB" id="2500928at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="456" mass="51176" checksum="C5420E99C95EE6CA" modified="2019-11-13" version="1">MRSTKGGHRASSLALPTCPFPHNSPPAHASYLQLPKQPYRPNIPNLVMSIPETATALAEALRKLAADSYNMQDEHIIVQKNEEVPSEEDVKTHKDLLNQVKTTLLPETRSLLISLQEALDLFPESTAPKPKLDDALNAVSRFHTVLTNLNIAVYTISPQIIALGRKSSQIDQTYGVLKRFRTYILEMDVREITSEFLSDLFTELSEFVSSGQYAQSSSEYPDEEEYATLILNRRTQLLAAVDKSQRKIDEALELIDRSDFDILRYIWTEYEQELGDYLTKIAQKIHDAKDVDIPPADQPLNLRPRIIQLLPQVIPFVKLCRVFYKRIGSAPPAFTFGEELSSADLESIRLGAGKINNSLIYALDALFSAYSNPRGQRTSSASSHFDAVKKAMDSTLEFIASHMVPSDPARTVDDVMNEFFGTLKPQFDALHERFSAALTQFQQENERLGNLAGNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCZ1</accession>
    <name>A0A5B0PCZ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCZ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="258" mass="29683" checksum="D91F1C53DB08026E" modified="2019-11-13" version="1">MRPDKYFFEEETDRFSCILCGGKSTTNQKKHARGERHQNLVRLDKERLQRQQERIAANEALNQDESNRPRVEIETITNTDLQNEQLQPLSAQNDDESDVVPINLFEDGESEHEEEEMDWLNLLGVAIDQIDDEPADSFEEHPVPAYQRDVVEIEEITTIDSPWFPFVNKEHINIMSDQFARVGCVEEYPEEDTANESPLSGRKGISIWEANVCARSQRDNLRRFKESLGRSPLGFPAGRHTWKGHIQDFTVGKVAQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PG80</accession>
    <name>A0A5B0PG80_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PG80"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022711930" evidence="1">
        <location>
            <begin position="23"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="166" mass="18539" checksum="464532B2ABD5B28B" modified="2019-11-13" version="1">MALHRVVLVVVISLLNLQGSLQQTKPPSLTDIKCGDKKEFKAGDCAAAYRKINYDKDSTLDINESNVERSSESCITRINNPKFVNVPKAIIENGFDQILAKCNGYSGSATLPGFNGVQLLTSHHAGPDFRSYDDYKEFNRMICNGDFPKGRKVLKEDCMEYVSFHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIQ1</accession>
    <name>A0A5B0PIQ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIQ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="174" mass="19447" checksum="4A6D6A4DD37396DD" modified="2019-11-13" version="1">MHASNDSQYNKIPECQGSLITEQTASISLAEMVVSKAAQSQRARRAIESEEKIQLQRSRVQKPKRLRQPKVPPIEIVDEGQPVPVEDDEPTVVVVQAVHSTQLAQWSTPSSPSRFIPHNQLSGHHHPSLVNTSNNNNTNTNTNTNTTTNTNTNTNININNNQQFHYLSRIIVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJW0</accession>
    <name>A0A5B0PJW0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Beta-galactosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_016351</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJW0"/>
    <dbReference type="OrthoDB" id="408320at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051913">
        <property type="entry name" value="GH2_Domain-Containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Ig-like_GH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42732">
        <property type="entry name" value="BETA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42732:SF2">
        <property type="entry name" value="BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="beta-Galactosidase/glucuronidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolases family 2 sugar binding" evidence="5">
        <location>
            <begin position="15"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 immunoglobulin-like beta-sandwich" evidence="4">
        <location>
            <begin position="127"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1101306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="34931" checksum="6AF1BABEFEE09A8D" modified="2019-11-13" version="1">MESGLSGIMKSHIYSWYRRTFVVPEHWSGRNVLVNFGAVDYEATVFVNGKYVGFHRGGYFRFTLDLTDALKEPGEENELLVFVYDPTNSKGEVIPLGKQVLKPNHYFYTPTSGIWQTVFLEPVAKEYILDIVTTAHADGSVKIKVITSNHASNSELKITVYNPIISSSDSPLYPVTLGTPLQQHQGTANSLVTFTLPSPQLWSPTSPNLYHFRVELRQDTVQSHFGLRTIEKKKDRAGIVRPFLNGEFVFQLGTLDQGFWPDGLYTAPTFEAMTYDLKVLKLLGFNMVRKHIKIEPDLYYYACDQMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLJ1</accession>
    <name>A0A5B0PLJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLJ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="12016" checksum="1F040500AC51238E" modified="2019-11-13" version="1">MTYPFQNPLTSYSGPSSSTHHPRATSRERSSAGLDSISHRPATEYLRIVVDPTNHGQSVKPDEIGSGSGPLSAIFLLSAPERTSRYSIQRQLASAHLGAAEWMRLHGARGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTK1</accession>
    <name>A0A5B0PTK1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTK1"/>
    <dbReference type="OrthoDB" id="2507227at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="246" mass="28012" checksum="54E49493F6C10A7D" modified="2019-11-13" version="1">MTEYNLICRPTSHRLALLLWISILVARSLRIIETNTDRIVATLIETAPSYRAETYSYLSVLRLLKPMASTLEIATRLNRYTKIVLLLYLLQHLLIAIVYLPISVIALRGLRKRAVPKDLLQRGSVQICTPTHQDTIDKVRKRLINHALLIYLQEILYCPTLVYMLCAPSTKLTHFSDPTWVLVEQVAIHGPIALIGNIILSFLIQNALYAARSSCNHLHPVDTDTRPKSVLEIKLKTEQHKETCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVC3</accession>
    <name>A0A5B0PVC3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="9">1.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_029007</name>
        <name evidence="15" type="ORF">PGTUg99_004337</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="15 18">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the stereospecific oxidation of D-lactate to pyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="9"/>
    <dbReference type="EMBL" id="VDEP01000349">
        <property type="protein sequence ID" value="KAA1097215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVC3"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000038">
        <property type="entry name" value="D-lactate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="14">
        <location>
            <begin position="161"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1097215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1104648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="610" mass="66764" checksum="9DAF16D2860889AD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1P5</accession>
    <name>A0A5B0Q1P5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="3 11">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_001805</name>
        <name evidence="14" type="ORF">PGTUg99_008543</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="3 11"/>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000147">
        <property type="protein sequence ID" value="KAA1127907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1P5"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000012">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="12">
        <location>
            <begin position="17"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1107086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1127907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="329" mass="36516" checksum="420492B584228BEB" modified="2019-11-13" version="1">MSDSPPTTTEGGLPTKLLVDKHIQYIQSLDQNRESLAYHLTEHLRMNGVYWGLTSTLLLDRPDGLPRQEMIAWVMSCWEPESGGFRPHPGHDSHIHSTLSAIQILTMQNAIDVLDKPKVINYILSLFDPERGSFSGDSWGETDARFSYCAVSGLALLGALEKLDHERVVGFISRCQNFDGGFGMVEGAESHAAYVWTCVGTLAILGRLDMVDKNTLGWWLSERQVPNGGLNGRPEKLEDVCYSWWALASLVIIGKAEWIDRSKLTSFILSCQDPDSGGIADRPDDMPDVWHTVFGLAGLSMLGYEGLKEVDPVFCMPAQFTKGLDQDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6P9</accession>
    <name>A0A5B0Q6P9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6P9"/>
    <dbReference type="OrthoDB" id="2495363at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="459" mass="53876" checksum="4DB8308C0EFCB9D1" modified="2019-11-13" version="1">MGGTSSSPPTDSTWAIQKLSNIESIISYDELINQILKEPLCFHEPTLHSQFSGPNFSPQANHQELNDKMVYSPQQLTASDQGLPRPPKRKWHVILTEGAENQSPSKQTEIVEECPKSPAEETRSMLLEGADLDPDEIMRKNEELMRNTSDFVPHAQLDMQEEKSKEPSIPTKLFHVYNWEYVKQSPQDQTTEGNGVLNADRLEELFSLLNSCKTIPPQGPLFWIPRETVHKFLKRYRKLRLQSPGRSVHRERKRVLGKLIAELCDEKLDIEKYPIFSDEIIKSLRDRLALRLKTNQTSNETRQNDNRLLRKVQKIIQYITNATKISVFLIITHLSLFKEHQNEIISREEVNKILCFMRDLWKDMEQEELLLEPSKYTWIKRSSDMFRLEEKQDDLRNEKAWANGYSLWYFVAEDIVKYWTESNRNKFKLRWGKREYPSEVSQIIELIISYANYETIVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAG5</accession>
    <name>A0A5B0QAG5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAG5"/>
    <dbReference type="OrthoDB" id="2496664at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="505" mass="56631" checksum="188C5B0E994FE1E3" modified="2019-11-13" version="1">MENTQKITRSKTGTRKEADTTSQAAAQSAAIDATTLGQDRLATKGPPSKEVINLLGRNAGETLPSETHDSEGEESLDLITKVPLLTAETLKEVVDTTSVLPEPSVGDQRSHIWEKIKEAQAAGDTILTKILVTAYKDLEDSTSTETPKLARSSSALPVLSTVEPKTVIATSVTVTELEDNLVYAVGTVTSHQDIGFTPYFDENIKKLKAPLPLTIFDREWQKKAIAAHLTIKPSKSSEDKAYRGLAYHDEWTQSHSSWTNNHRSFYIMLRDVYNKKLFAEKLRIHKENCDEIADVYGFMTAFRYDMQTRMNAFAHRVPSKDGAAIPDISVKQMVVVEQCYSTVRSLGEASWKDNNYAPGLSHAGFDPDTGLRRPDFHKSASYQSSFNKEKQQQPNHQERGREKRWFNENAQQNMGNRPFGFDNRNPYQNFNNNNNNQNQFDYANYNQQHYNQHPYNSPYQNNQGSANPNRGFVGGSKRFKGANSNFDSGARGGGSKDEPNVTNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAU7</accession>
    <name>A0A5B0QAU7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAU7"/>
    <dbReference type="OrthoDB" id="2497263at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="219"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="430" mass="48489" checksum="3092316DB28A6752" modified="2019-11-13" version="1">MSAAETISSETTALVVGALEKLAQQSVNIQDAHHVVEIEEQVRSSDEMVARKALLTQLQTSFLPSLQQQLADLMKSLNLSPSRIAPTPKLIDTLEIAFKLGNTLKDIGSSANTLAPCIVATHQKSSRIDEDYGVLKKFRCDALLSKINNLISHDLKKLFQAYLVFVRRGQFSIDNTTSSADESDVLHRRKQIIEKSEQVSNLIEGIVTWSRRSDFGVLQDEWEDQANKISQSLAKLTERMNSARRLLVEEIDAELDQPEPEINQAVDTRSETDVSDDSISYKFRIRSLVIYLMQLAVPLVKLCRLFFRKLITSTSKPPFTFGEKMCSADMKWFQQKACPIALDIGIILKTLYQIFDANALNGRIQHMKNVANQLIAHLDCSLVLLSFHLVPSTPLPAPENLFKSYFSPLRYEFRSAMDSFLNGLHSLERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBD1</accession>
    <name>A0A5B0QBD1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycerol ethanol, ferric requiring protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GEF1_4</name>
        <name evidence="3" type="ORF">PGT21_024437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBD1"/>
    <dbReference type="OrthoDB" id="5852896at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04180">
        <property type="entry name" value="LTV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="69"/>
        </location>
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        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="215"/>
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        </location>
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            <begin position="39"/>
            <end position="48"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
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            <begin position="255"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="2">
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            <begin position="270"/>
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            <begin position="317"/>
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            <begin position="519"/>
            <end position="532"/>
        </location>
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            <begin position="533"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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        <location>
            <begin position="579"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1110538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="591" mass="65937" checksum="AD916324BC87963C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
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        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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            <end position="23"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1087421.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1110520.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1110524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="174" mass="20212" checksum="FE546D8219C8714B" modified="2019-11-13" version="1">MPPFEGGGGKKPKRKRKPTTASGVAFSQAVELEQLSTRGEATTIHLDHHIPDRSGFDNFDQDHSNPYDHHFTDYTGNDTNFTNSDNTTHSDTRIQQLTDYFRSEHYKRKKLLEEKNWAEVYDAMFSSFKQCAVKTADWGDEDKWRHDWKEPCLCQDKRFRPLVLVDIMSMCSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
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                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
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                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="EMBL" id="KAA1111003.1"/>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEF4</accession>
    <name>A0A5B0QEF4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <end position="580"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAA1111567.1"/>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEG1</accession>
    <name>A0A5B0QEG1_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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            <taxon>Puccinia</taxon>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEG1"/>
    <dbReference type="OrthoDB" id="2508562at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="644" mass="73607" checksum="2185DD72B55FE124" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QI15</accession>
    <name>A0A5B0QI15_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QI15"/>
    <dbReference type="OrthoDB" id="2499505at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="2">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="285" mass="31465" checksum="1105C9304F994253" modified="2019-11-13" version="1">MVAFAQPPMRKAHQKLSFSPGSHRPTEPSSGPPLSMNASKLDKLKKLWDRRPKFAGPNGGGNTTVTVASLTLPYSLSSKSKNKKQSRRTTIGTEKIGLPHDFIHSKHVGIHDVREIAYQPRHSMGAVDMLDSAGLRSSLYRLTDSEPESQSEEEKTPAAVTQRRSPQERRSLGCPGPQVPPSPRSNKVKRKSVPIHILQDVPESPPQLPSADRPGEAAQGRYRFSCLSIEESVKELINYAQPESLVDEQGRPLHMVGGEYMTQTMKGKWDNKMAEIAQTLKSEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMC3</accession>
    <name>A0A5B0QMC3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMC3"/>
    <dbReference type="OrthoDB" id="2515693at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="245" mass="27742" checksum="35A99542780697BD" modified="2019-11-13" version="1">MFLQDDPANEPSSPVPHEDQSLPENDSTMIHDIDQGVMQLPSPGRLNVVPRVAQDLLGRINFSAEDVELINELLMVPQFDQWRVSVILMLANLRRVGMRTDAEAPAPTEALLGNSFVFSNTIHNFFRIKIRELLTRGGLEAYARTHTMGGLPIARSPLSLLTTYLEEQPQAFREDYLPQGWPMNHLANQSMLTLMRVLVKHERGSLRNLEFNRRAINGPVPNLSNLILLIDRAMGDRDHLRPLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMR4</accession>
    <name>A0A5B0QMR4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Adenosine 5'-monophosphoramidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">HNT1_2</name>
        <name evidence="5" type="ORF">PGT21_013902</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMR4"/>
    <dbReference type="OMA" id="YRVVMNC"/>
    <dbReference type="OrthoDB" id="672793at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648:SF1">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="HIT FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="28"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="116"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1114566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="169" mass="18692" checksum="37D6632BF9CEC86C" modified="2019-11-13" version="1">MTAENECEPNELATQEELIKSKADDECIFCKIIKGAIPCFKIFESSTTLAFFDINPISLGHALVIPKYHGPKLHQLPDFALQDILPIAKKIAMELGLTDYNILQNNGRIAHQEVDHVHFHIIPKLSASDSEGLVIGWPSKKADMQLLDQKAKELKAKLDSQSASSASNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNJ0</accession>
    <name>A0A5B0QNJ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNJ0"/>
    <dbReference type="OrthoDB" id="2506388at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="508"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="311"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="553"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="613" mass="68997" checksum="AF1CED63E465E4C2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPG0</accession>
    <name>A0A5B0QPG0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ELMO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPG0"/>
    <dbReference type="OrthoDB" id="10637786at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022775552" description="ELMO domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="706" mass="80823" checksum="1C9DBB07C967A892" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSW3</accession>
    <name>A0A5B0QSW3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSW3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023042221" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7370" checksum="285CE6A95E8C1ABE" modified="2019-11-13" version="1">MLIKKALCFLATPSLIWGIRRDQNPVEPVCAHVYRQTVFIDAPCGFNYRCERQHVHVCVATCLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QT57</accession>
    <name>A0A5B0QT57_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QT57"/>
    <dbReference type="OrthoDB" id="10268468at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022926147" description="Secreted protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12422" checksum="E8B797FDA620A43D" modified="2019-11-13" version="1">MSVKKAIFLFAAACLLSAPMAKGSPSMAPTSSGCAHLIRKTVYKDFPCQATYVCLAGYSHQCGHGLTGQAEKCENCKEEDSIELWTSCPYVGHPPRQCAYHNVPVGARRKRGGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVI2</accession>
    <name>A0A5B0QVI2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVI2"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34800" checksum="AD2C69A7478CB148" modified="2019-11-13" version="1">MKSQAYPESLNVAASTSGSQNLPLDIREVVYNFIATINSSASPINIDAQAERILQASGMCDDPINFQEVERVLQDLTPEDRLAFERLFTNMREAYLELANNPDRDCFSEAEYARYFGDLEQVDLVLEGPNMEKSDVEIVEHLLDQGFSIMSVMEKSRQRSFGERNIQALLENVTPDALATNDRDDENPQCLICREDFLESNLVVVQLPCHSTHQFHRVCINVSYVQRLFKFHLIPIPSCQLQNRVAGARFGKRDMQESVAYVTPAVLTSNDLDGGNSQCQIRRGTFVESNLVSVQFPFHSTHQFHCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWB6</accession>
    <name>A0A5B0QWB6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016168</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWB6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="93" mass="10294" checksum="A694B4C43B21D8B7" modified="2019-11-13" version="1">MSEARQGKPTPICDTRKTLGSGSLGSAGPTSRTPLGWAFQTLQINRNFHKELKFLFMAFAILLSTSFTPMPEIIWGSRMKPSYSIQASGRFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWK5</accession>
    <name>A0A5B0QWK5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWK5"/>
    <dbReference type="OrthoDB" id="2509423at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="180" mass="20673" checksum="FBDC33FDA38CA852" modified="2019-11-13" version="1">MNRPEKASKPLPYCHHHPYHSSHSTRTPKEVVRRPSNPPSHENNHLTKPSDHPEQFAFNINQPKSKVSQSIILKGIPGLADNSSVFKFLESAGAAVCLVNVIYNPKTSRSRRYAYAHFFGLDRAKEFVKPRFPLMSWKEPDNSSHGDYYRTLKIQIDYSTEPPEVKQNRRNDGDDDVDDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZB5</accession>
    <name>A0A5B0QZB5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015979</name>
        <name evidence="2" type="ORF">PGTUg99_009099</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000246">
        <property type="protein sequence ID" value="KAA1118638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZB5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="84" mass="9457" checksum="C1A99F1897F98243" modified="2019-11-13" version="1">MRVCCAAAKARQNTENAVNDKDDIQIISESNKAERLRDEATAKFLEKALKAEGEGDKARSDMFYNLYAAAERLKEEIQSFNAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R8N4</accession>
    <name>A0A5B0R8N4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH:ubiquinone oxidoreductase intermediate-associated protein 30 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_009884</name>
        <name evidence="7" type="ORF">PGTUg99_009381</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CIA30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1121960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R8N4"/>
    <dbReference type="OrthoDB" id="42561at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010257">
        <property type="term" value="P:NADH dehydrogenase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013857">
        <property type="entry name" value="NADH-UbQ_OxRdtase-assoc_prot30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039131">
        <property type="entry name" value="NDUFAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194:SF18">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08547">
        <property type="entry name" value="CIA30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="5">
        <location>
            <begin position="41"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1073360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1121960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="246" mass="27533" checksum="FB7AA0948F9567B3" modified="2019-11-13" version="1">MASALKARFQRTVQSWHRYGLAVARMDPLGGEPYRRPLPIFEMSHPSHLDQFALGCDADMGGYSTIQLAIQQSPDHPFPHGVFRGTLRSELGEQALKSGILKAGGYAGFRTRVREMLIGEDNWNAEQHEFLRLRVKNSGDGMKYFVNIQTEGPVSTDIFQHRLWLGTPGEWEDVLIPLADFALTNKGQILNNQFEMPRESIRTVGISVLEKPGPYELGILKVDCVSEIGLKLEEQEESQSSHSVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RGY1</accession>
    <name>A0A5B0RGY1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017799</name>
        <name evidence="3" type="ORF">PGTUg99_027115</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RGY1"/>
    <dbReference type="OrthoDB" id="10546509at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="125" mass="14315" checksum="F39031889260AD68" modified="2019-11-13" version="1">MFDSLKLEKGNRVIHQSHKNHITTLSLENFMRMAHNNDILDVYPKPKEDSAGHQVPDCEDTLHLGLRKLQDEISGQHPNLNWFKLHFMWEAGPGESNASLVQEHPTTVDAESFNNDDRPSSPFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RLF6</accession>
    <name>A0A5B0RLF6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CDC60_3</name>
        <name evidence="15" type="synonym">CDC60_2</name>
        <name evidence="15" type="ORF">PGT21_021376</name>
        <name evidence="16" type="ORF">PGTUg99_017933</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="16 18">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000175">
        <property type="protein sequence ID" value="KAA1125773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RLF6"/>
    <dbReference type="OrthoDB" id="10249672at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000001">
        <property type="entry name" value="Leucine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055416">
        <property type="entry name" value="RBD_LARS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24810">
        <property type="entry name" value="RBD_LARS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="71"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="702"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="834"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine--tRNA ligase RagD-binding" evidence="14">
        <location>
            <begin position="986"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="149"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF24810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1070699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1125773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1135" mass="128023" checksum="F072434BE4629499" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RLI3</accession>
    <name>A0A5B0RLI3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SGS1_70</name>
        <name evidence="11" type="synonym">SGS1_152</name>
        <name evidence="12" type="synonym">SGS1_34</name>
        <name evidence="13" type="synonym">SGS1_8</name>
        <name evidence="13" type="ORF">PGT21_003336</name>
        <name evidence="12" type="ORF">PGT21_012528</name>
        <name evidence="14" type="ORF">PGTUg99_015448</name>
        <name evidence="11" type="ORF">PGTUg99_033058</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RLI3"/>
    <dbReference type="OrthoDB" id="2499463at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22265">
        <property type="entry name" value="UDM1_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0347">Helicase</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="58"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="291"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="676"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1074287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1076590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1090497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1126756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="727" mass="80562" checksum="102DBA4FB81CB281" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SIJ2</accession>
    <name>A0A5B0SIJ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025925</name>
        <name evidence="2" type="ORF">PGTUg99_027258</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000035">
        <property type="protein sequence ID" value="KAA1136354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SIJ2"/>
    <dbReference type="OrthoDB" id="2495286at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1136354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="251" mass="28467" checksum="F8D02548C7CB0224" modified="2019-11-13" version="1">MYAKKSTFFQLFRVSTLAIIHSAIGAIFTSPVFDDITTLKRISSKWKLTSTQSEETTGFGKQFLDEHGELCFLYNTHVQLTARVVDIYPRQDVIPTREDLEKQIDRPNQPHQNPPVFSLPALQVFNPIRGSVVQLAIIDMKNGMKIEDQKIRGTLPAKYYELPEGYDPRRILFSVDAITDHCLGSLMKSQPTKPEFPSHSSLLTSDTIGFDENAPDSIIQHFNIFGLIWNCGWSWIKKTMDFINSPASPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SIP6</accession>
    <name>A0A5B0SIP6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NudC domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_005712</name>
        <name evidence="9" type="ORF">PGTUg99_015755</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SIP6"/>
    <dbReference type="OrthoDB" id="428655at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037895">
        <property type="entry name" value="NUDCD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21664">
        <property type="entry name" value="CHRONIC MYELOGENOUS LEUKEMIA TUMOR ANTIGEN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21664:SF1">
        <property type="entry name" value="NUDC DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="7">
        <location>
            <begin position="345"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="309"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="513"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1067445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1137772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="780" mass="87838" checksum="238A656778235755" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJT6</accession>
    <name>A0A5B0LJT6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MAK5_1</name>
        <name evidence="2" type="ORF">PGT21_000012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000201">
        <property type="protein sequence ID" value="KAA1063898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJT6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1063898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="74" mass="8631" checksum="5DA0142C331D6A64" modified="2019-11-13" version="1" fragment="single">RRSSGPIERKTRRPIDHQENSLIIRSKANHQHRPRKINNCFIKDPQVIKNFLSNQNHSHLVGLDRSVNALNELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LK23</accession>
    <name>A0A5B0LK23_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_009371</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LK23"/>
    <dbReference type="OrthoDB" id="2505480at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22744">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF11">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN DDB_G0284757"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022769534" description="OTU domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="OTU" evidence="3">
        <location>
            <begin position="163"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1064609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="253" mass="28119" checksum="A83938B14DB7EB79" modified="2019-11-13" version="1">MRSGTVYPFLVLFFLFLFHEFPSSLGSFEQTWHDVDGLKYKWSYSRKLSDYVKTVVHDSSSRRGQNVWIDQRDGLKHQWTLSPVSNEWIDTALESTITSASTSTGIEGAGGSNCPVSPVRSPEANLSPNETPAQEYATQISSKPPRKADLRDLDSRGLPQYTYTKRLIKGDGNCLYRALSYLANGNDQKTNGEWGGEHALAAAAKVLNKKIILLSLASKDPVFLAYGSAPVDPEAPGLIFLNEHYELLFRKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLH8</accession>
    <name>A0A5B0LLH8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF1264-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024385</name>
        <name evidence="4" type="ORF">PGTUg99_020368</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OBAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000277">
        <property type="protein sequence ID" value="KAA1112497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLH8"/>
    <dbReference type="OrthoDB" id="1901244at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010686">
        <property type="entry name" value="OBAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31360:SF0">
        <property type="entry name" value="OIL BODY-ASSOCIATED PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06884">
        <property type="entry name" value="DUF1264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1112497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="26314" checksum="DDBD6FEE333B9C26" modified="2019-11-13" version="1">MAPWSVLETPMAKIHQHLCAFHVVADEHGRQIESHHYCTCVSPDFHQCIIYDSDKPNARLIGIEYLISEDKFNNLPVEEKKYWHSHKYEVESGVLIQMHASGIEGQVPENKEREMMSAIHKTFGKVIHTWNIDQHPDLPLGPPSLMMSYTKPEMVNWEQVKERDREQGVNTQERSKIRSTYLDLDYPKNKEADQWMRSGKPIQFKPVESSLTQTSNGKQASNGNRNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMH0</accession>
    <name>A0A5B0LMH0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033173</name>
        <name evidence="2" type="ORF">PGTUg99_010251</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMH0"/>
    <dbReference type="OrthoDB" id="422106at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="577" mass="64132" checksum="13ABF02844FC7E1C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNG5</accession>
    <name>A0A5B0LNG5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031093</name>
        <name evidence="3" type="ORF">PGTUg99_028997</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNG5"/>
    <dbReference type="OrthoDB" id="2505088at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="461" mass="51593" checksum="B7CAEC3841C1C07C" modified="2019-11-13" version="1">MADNSFSFIDQLRPCLHTCFPCFGQRSSNSAEPAHHKYFPDSDHRGLLEEEEEGDEEQGIHSAPHSEYQFQSNSQLSRWDLLISWLSSKTSSSSKPTRPFDVDDLQTSALSHNGEADALLLDDISIALRARSSSRRMSTKSKSNAPTLHQPQPIPSPSVTSNHRRKSRNPEPNSSTPNQHGSRSLSSAPVKKKDTRSLVEDPQQKSLKDLASLSSTSHPKRSKSRSKSSKQSSSAYTDETVDSEPRSVPSLITSRTTTTTSSSIPSSDRLELNVEIIKFIHQNYNQECRRLYGRDLSRLQIQQLCEYLISRRFNDPIGPRPGPSSPATGAMPAEPDDKKRQAETDDPAKENQTPLSDSTPSRPSLEIESLKRIQLSSTEFLQAIKQTKTSHHHHQQQQQQQLHPSETDDSETQSLMEDPIEVELDQDQDLFLSSSLLDWFTKGSQLTSDILNNLSVNQSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQQ0</accession>
    <name>A0A5B0LQQ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Subunit of the glycosylphosphatidylinositol transamidase complex-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GPI16_1</name>
        <name evidence="4" type="ORF">PGT21_023578</name>
        <name evidence="5" type="ORF">PGTUg99_006244</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQQ0"/>
    <dbReference type="OrthoDB" id="331263at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007245">
        <property type="entry name" value="PIG-T"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959:SF11">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04113">
        <property type="entry name" value="Gpi16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137031" evidence="3">
        <location>
            <begin position="23"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="521"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1066160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1072639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="601" mass="68782" checksum="4A08636049D1DD83" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSP7</accession>
    <name>A0A5B0LSP7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">N-terminal acetyltransferase A complex catalytic subunit ard1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ARD1_3</name>
        <name evidence="7" type="ORF">PGT21_034185</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSP7"/>
    <dbReference type="OrthoDB" id="5363962at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08234">
        <property type="entry name" value="threonine_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000843">
        <property type="entry name" value="D-arabinitol dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="6">
        <location>
            <begin position="34"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="5">
        <location>
            <begin position="190"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1066594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="363" mass="39579" checksum="550F5A9CA0CB7DD6" modified="2019-11-13" version="1">MAAAAVMPSKMTALYYEKARHFSVIQADLPSIDENEVLLKVSMCGVCGTDQHIHEGEFISKFPLIPGHEVIGTIVQAGSKVHSVSIGDRVVCDVGETCGKCFYCQRGNSLFISTVTVRTIRFCESFEAHGVTLNGGFAEYAKFHAAKVFPIKNLTDEQATLVEPASCAVHGLDKIRPKPGSECLLIGAGPTGLILAQLLKLNGAQRVVLAANKGMKMDIARKIQAADEYIDLDRADAANQWAQLKKDNPHGFDVVVEATGVESIVNDSINYVRRGGTLLVYGVYDNAARVSWSPTKIFSDEINIIGSFSQTNCFPRAVAYLDSGRIRTEGMVTDIFKIEEYQQALDKMASRQCLKIAVRPGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUF1</accession>
    <name>A0A5B0LUF1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005587</name>
        <name evidence="4" type="ORF">PGTUg99_016415</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUF1"/>
    <dbReference type="OrthoDB" id="2509383at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="196" mass="21722" checksum="470EDB3ADF825FC2" modified="2019-11-13" version="1">MKDQVRHIAVLLVFAMMVNLNIAMEIIKSMRANGKAIKDSGTTKLNAKQQTLPGPRSSGSATKMMGECSCSGAIDDEETPVPGSPSVGPATIMVDKKRKKLVLKSHMDGVFCNVVNQKTNRPILKEVELKKDKLYPLNAELDEKIVLNYKWTTSPKLGSKPVKLWLSLSKRLKKVYLTNSQEAEAPIDRFALIKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUF9</accession>
    <name>A0A5B0LUF9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015077</name>
        <name evidence="3" type="ORF">PGTUg99_026741</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUF9"/>
    <dbReference type="OrthoDB" id="432953at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026705">
        <property type="entry name" value="Hid-1/Ecm30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575:SF12">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12722">
        <property type="entry name" value="Hid1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="889"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1161"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1246"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="899"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="920"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="955"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="973"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1161"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1246"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1257"/>
            <end position="1266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1266" mass="136763" checksum="C5FE0561A30169D0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUN8</accession>
    <name>A0A5B0LUN8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011538</name>
        <name evidence="2" type="ORF">PGTUg99_021121</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000506">
        <property type="protein sequence ID" value="KAA1068142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUN8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1064679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="117" mass="13287" checksum="7B0C9EFD8AF03DD5" modified="2019-11-13" version="1">MRRENLSAFIVLMLCKFEIHRIQGKSSDLKDGAYDELACAGSGGQRGPAFAGFLGRIWPPLRLSPATRRHGRRFFKPVFRGHYRRRQLIENELPHQLGYAVSPVGVARQINDPLSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYG0</accession>
    <name>A0A5B0LYG0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CCR4-NOT regulatory complex component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CAF16_3</name>
        <name evidence="4" type="ORF">PGT21_004392</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYG0"/>
    <dbReference type="OMA" id="YLGTEWV"/>
    <dbReference type="OrthoDB" id="6512918at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001332">
        <property type="entry name" value="Similar to ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158:SF2">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="15"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1068870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="312" mass="34924" checksum="972F0D6DB48FD5BF" modified="2019-11-13" version="1">MNDRGQPELAATPAVEVSKLFFRFNPESLDVLRDVNLDLERGSRCLLIGANGAGKSTLLQILAGKRMSKSKAKALGEDVFFNTPAGVAYLGTEWASNSVVRSDLVVSHFLDSVGGYRHKSRRDRLLNILDVDLDWHMHQISDGERRRVQIVQGLMAPWNLLLLDEVTVDLDVMVRQELLQFLIEDTKTRDCTVVYATHIFDGLDNFPTHVCHLQIGSTTTPKPIPWPISPETAVQNDCGIPPEALHEIASPDRIGSKLLVLALHWLKEDRLIRLKLEAQGDPAFKKRGALPESSVPTDSEVFYRKYDYTNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M048</accession>
    <name>A0A5B0M048_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_020466</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 9">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M048"/>
    <dbReference type="OrthoDB" id="2504739at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="160"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1069308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="287" mass="32033" checksum="F8ECC8E076DBD26F" modified="2019-11-13" version="1">MLALIMVDEGHMIYLWGLVASKQSKGLIIFARLEDQAIFRPAYGHIGTRLMATNNIPILLLSATCRPEAVDKITTSLMLSPSNLHMIDGELTRPEICFIRVYMDSTLSSCDDLLRLFAPHTKTVAEDSAPMIIYSGTRNRTFQVMKVVNKAWHTQKHEYDPNDQFIRQYHLVTGDEDKARTVEDFGNAQVPVILATMALGLGQNLKRVRCVVHMGQGDPAAIVQMVGRCRRDGNVGLGLLFMERTRKNGKNDVEAFGDKVHQDDDACMDALTVTPCCLRIALALDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2V2</accession>
    <name>A0A5B0M2V2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008601</name>
        <name evidence="2" type="ORF">PGTUg99_032221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2V2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366074" evidence="1">
        <location>
            <begin position="28"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="143" mass="15809" checksum="E5B46CAC53C42D32" modified="2019-11-13" version="1">MPNLSCTKLIQLGILLSLILLSHLAMCARRGQPNNPGNVGKRCGNCLDLHLQGRGPAIPLQQRTQLPQGIFETLSCAILQSPQEGCHRDIQVQEFQCRLCGWYAWVPTGDCPVHPRAPPIYCDSNDINIIKQGEAVVDTELRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6I7</accession>
    <name>A0A5B0M6I7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023839</name>
        <name evidence="1" type="ORF">PGTUg99_020631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1071956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6I7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="144" mass="16261" checksum="73B1D43C83C98FEF" modified="2019-11-13" version="1">MSLQLSSCNRIQDQNGAVIMILDNKELNTNTRTCWLDSLYLIIYEFITKDRSEFPQLIIGAVYSSSLSTQSVAKEGRLPPDTARQFYGAIKAHEKILSHFLEAECETLEMELDTRSSNEKSPNFLPCGHDYNKGNVCGAKNYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCP4</accession>
    <name>A0A5B0MCP4_PUCGR</name>
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        <recommendedName>
            <fullName evidence="3">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_024010</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05137">
        <property type="entry name" value="RasGAP_CLA2_BUD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="3">
        <location>
            <begin position="1199"/>
            <end position="1400"/>
        </location>
    </feature>
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            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="371"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="493"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="518"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="682"/>
            <end position="726"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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    <feature type="region of interest" description="Disordered" evidence="2">
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    <feature type="region of interest" description="Disordered" evidence="2">
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="1093"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="1535"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        </location>
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        </location>
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            <end position="486"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
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        </location>
    </feature>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <end position="1934"/>
        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="2">
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            <end position="1971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1972"/>
            <end position="1986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2005"/>
            <end position="2015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1073740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="2031" mass="221889" checksum="87D4A15A2B576B7D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDN2</accession>
    <name>A0A5B0MDN2_PUCGR</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8180" checksum="7B44066E97057757" modified="2019-11-13" version="1">MTPLEAQVAGLTLDAAIREDARLDHKFDDEGCFPALGTAAVAALEDLDTQLLQNEIDRATEASSSWTGNLADDIAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEE0</accession>
    <name>A0A5B0MEE0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEE0"/>
    <dbReference type="OrthoDB" id="2507518at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="276" mass="31828" checksum="BB791AD92B73301C" modified="2019-11-13" version="1">MLIRNSDTAPRNIYWRGTQMMFQTWYHKGQNISQRSKPRQVYLTGELSMHLHTYLAFIRPTQMFLLRGLGEKTNAIDMQEFLWMGSRIGRLETPDFSRILKKYFLHGGCEGVGIRVWRQAAWIWCYADEELNFKLASQVWQAFWGIESLWASKQPPLPVPDTLPPGEKARQALTIFTRNPNALRTRLTTKREITFRPELQIDVQPKYASTADLVPILQGLTQSHILKKKDRAMIFIEHRESVALIAQAVGGLMHHSGMSDENNPRVQASGSNETME</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFF0</accession>
    <name>A0A5B0MFF0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFF0"/>
    <dbReference type="OrthoDB" id="2495163at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27451" checksum="192A9338E13064B9" modified="2019-11-13" version="1">MPRTPTLEHVYFTGDRKEFDRFIYHIKDALSEGFDVNYGNGSEKMQINWIARHFRYPNGQNSMEEGPLSYNWWIGLLEKNARVQNLPISHAVSPADNYKIPELATKKAFLEHLHEAFGSPDAREVTRKALYACKQGDQDIQQFNLVFNTLVYAVDLTENERCDVYEERLDVDLLTTAIKHTGWRNAKTLHDKQALARSAAFILRKLAQLDLEKQNADNRDNQQPNILQHANRQPLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MG17</accession>
    <name>A0A5B0MG17_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025792</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MG17"/>
    <dbReference type="OrthoDB" id="2504071at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="293" mass="31747" checksum="276CE5187E6592C7" modified="2019-11-13" version="1">MPSDLRKRKAESSGLPSTVSNPKPAANEVGHAASNRQKPTIAPTVFMLLIVGLMFALFPIFYYTQVMLPDPLSTSILSNFRNKGNTILNALGHTSSAGNGRIPFNSEHKNQPPPASKGMAPPIIEQENKDSLPPSGSLGRQTTQPPDTMAKTSDPITTSTTTTTTTTTPSEKLSPEKQKKLDEELGLFWEASSRYRREAQKQKAGQYALQGDGTAPPPQKATTATAAKEEEEGAHGEQPLSLDRLPDADPDAKEGLTPEFWDAIKHQFSADDLTLVLNDMRRTEQAAAQSLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGE0</accession>
    <name>A0A5B0MGE0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clustered mitochondria protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Protein TIF31 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CLU1_1</name>
        <name evidence="3" type="synonym">CLU1</name>
        <name evidence="3" type="synonym">TIF31</name>
        <name evidence="7" type="ORF">PGT21_031961</name>
        <name evidence="6" type="ORF">PGTUg99_032997</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">May associate with the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CLU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="771227at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15466">
        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2280.10:FF:000002">
        <property type="entry name" value="Clustered mitochondria protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03013">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007967">
        <property type="entry name" value="GSKIP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF6">
        <property type="entry name" value="CLUSTERED MITOCHONDRIA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05303">
        <property type="entry name" value="GSKIP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Clu" evidence="5">
        <location>
            <begin position="427"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="211"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="601"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="800"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1101"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1537"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1565"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="827"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1144"/>
            <end position="1155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1076005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1091355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1597" mass="175884" checksum="2EDDE8DA791D7101" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MK46</accession>
    <name>A0A5B0MK46_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006812</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MK46"/>
    <dbReference type="OrthoDB" id="419616at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="12705" checksum="70D4F2D90795981E" modified="2019-11-13" version="1">MAEITDESNKARAFPLLPLCWAIGLMIGPAIGGYTAEPAKQYPHSIFARHLLRPLCFGGTLQHLGCRPGHTVSRGGTLPSKVLKPQKRSAAEISHQDSLEDQERPDPAERARTLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKE4</accession>
    <name>A0A5B0MKE4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2423 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010319</name>
        <name evidence="5" type="ORF">PGTUg99_008121</name>
        <name evidence="4" type="ORF">PGTUg99_013363</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000348">
        <property type="protein sequence ID" value="KAA1098661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1121954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKE4"/>
    <dbReference type="OrthoDB" id="4087970at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019434">
        <property type="entry name" value="DUF2423"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219:SF1">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10338">
        <property type="entry name" value="YBL028C_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2423" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1098661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1121954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="119" mass="13365" checksum="F3969EC2C2D5F4C2" modified="2019-11-13" version="1">MAKSLRSKSKRAFRATKRTSTSSDYQKTEAERLQRLSKKLTGSSAEGTEQAPDQEMQIDQGAENPDDSASKPAPKVTTHGPRMSGREVWKASKRGIQLRRAPSTTVWHQRSTGKPQRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML54</accession>
    <name>A0A5B0ML54_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_050092</name>
        <name evidence="4" type="ORF">PGTUg99_050112</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML54"/>
    <dbReference type="OrthoDB" id="2498125at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1126960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="470" mass="52890" checksum="2AAF53E373C5D732" modified="2019-11-13" version="1">MSLGTEQCASNPTDEAQSIGLQADTCRRRIRELSEALKEEQSRLERLEAIESSLIIHASLRSQQAGDESRPHEEGGMGPKRKAYQAVKPEVKELVIQNVFDKGMTQRKVSESFGLSERTVRRIIKAEKNIHSGLEPAPKRRRGAKTKLTSEIMSELLFKLERQPNMTLSDMKRYVSETHAVTCTPQSISRMLSSMDVNWKTTLKIPPHWNEINILRERHDFVVRRAIDVDKQLIFVGDTGFDLNFNRSQDPSCSEQGASLSLVPRTSQATLIGAMSTSGYVYHEIINPDGKRVTGLSREDYEEFLIRLSTKIDSRRSVVIVERAKLAGGKEEEEETRPLEGRLAEHFREPDSLASLLSSTHPPVGTHHPQASIIKTEIDYLPACSGFLSPLEISFVDLKTHIKSLDPPIHERSQLILHLTNAIHSLFPAPKPQDLFNFVAKELYPNCFNLAPISGPIIKHPDAFQSPPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML94</accession>
    <name>A0A5B0ML94_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML94"/>
    <dbReference type="OrthoDB" id="2505972at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="574" mass="65016" checksum="06710A768742212D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMK3</accession>
    <name>A0A5B0MMK3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMK3"/>
    <dbReference type="OrthoDB" id="2502642at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="513" mass="57823" checksum="A38C13FB1A7F8163" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPR0</accession>
    <name>A0A5B0MPR0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPR0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="146" mass="16793" checksum="22295E941F7CA1EA" modified="2019-11-13" version="1">MCRWISRAEPSRPFYQLSSSWRDHTKTLRAAQRRVCHKLDPRCRDMDESGRRPNGLGSVNHITRSMGRFIIYMSTSGIPGWGLLDVDTGWWTMAPDQEAFAGSRKHRHSSRQKSISRGRLERLHPSLTLQLQHIPSTSIPSQPTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPX5</accession>
    <name>A0A5B0MPX5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_027965</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPX5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023045952" evidence="3">
        <location>
            <begin position="20"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1078064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="321" mass="36022" checksum="F825286987A84038" modified="2019-11-13" version="1">MVRWSLLLPFFSHILIVECGERLTHLNLKSSERGEVAESSIFQRASNPVPVNEGHQRIPLQGDSEVLGHKFPAVGSSLNQRPEVSIRIDPKEISGDQWRPKQTYSHSNLNRSPHKSAVGWNPLDIENLGHSRAYSSQARNQISQFDSRPIMNRGNQPSIPSDALMHGEAVPVYRSSRTPQGFVVIVPLDPASEAQLENNLHAEAKTNWQSVNEIAATFRRGLHIFAADVLRTFGRTPPPPALVCFGLILLLDRLGTKEKIGLEKEANIRNGVRNNGSLSEIATSDLSSDSFNDTALPFFVSAMFLTWLYFFLKDFIRYRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPY4</accession>
    <name>A0A5B0MPY4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cytosolic iron-sulfur protein assembly</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CIAO1_3</name>
        <name evidence="4" type="ORF">PGT21_035245</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPY4"/>
    <dbReference type="OrthoDB" id="2674832at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="4"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="80"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1078453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="149" mass="16695" checksum="E2C943698AF9859B" modified="2019-11-13" version="1">MTLTSAHTHTVWLINWSPKGDLLASGSFDSTALIWTNHKFDQRLDQADAQDLEDKPGRARLPILDGEEEVKESWECLMSLKGHESEVKGVAWNRNGNLLATCSWDKSVLIWEILVGNELQAHQNVILDDEGYKLIAVLIRSQIGMLVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MW91</accession>
    <name>A0A5B0MW91_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_001987</name>
        <name evidence="3" type="ORF">PGTUg99_010239</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MW91"/>
    <dbReference type="OrthoDB" id="424465at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF21">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="304"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1103832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="632" mass="70666" checksum="6DAF89CDEBED6817" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0D2</accession>
    <name>A0A5B0N0D2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_008913</name>
        <name evidence="12" type="ORF">PGTUg99_015824</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0D2"/>
    <dbReference type="OrthoDB" id="2125469at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10951">
        <property type="entry name" value="CE4_ClCDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471:SF2">
        <property type="entry name" value="CHITIN DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="NodB homology" evidence="10">
        <location>
            <begin position="96"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1082627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1133474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="319" mass="35411" checksum="281DB0A6C55A2945" modified="2019-11-13" version="1">MMMMAQSAGMKKSKQWFTSMIRPVMILVILVLALTVVQPRQHTPAFVLNNIDADSQDARQVMGADANHNQLPFISALDTVRADFAVPVYTTCSAPGSFALTFDDGPSQFSSNLDGTLDGSNSKASFFINGNNFGCIYDYAERLVDRFNKGHLIASHTWSHVHSNEGSYDQIAFQLELIENAMIKILGVKPLYFRPPYGEYNEVVLQVLQDRGYRGLILWSEDSGDSLEAPPSSSEIIESYRSYPERTNVLNHETQSSTVNEVIPNVIPILKDKGFDLQTAPHCLNLGSNPSDWYVSVQPPGSRDETWTCDGTPMPGSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5Z1</accession>
    <name>A0A5B0N5Z1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5Z1"/>
    <dbReference type="OrthoDB" id="2515958at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="461" mass="46695" checksum="3F103DCD74FAFF94" modified="2019-11-13" version="1">MFVWCVHVIYSRSHQAAPISYCSIEIVASDESLERRSQYEFVRNTPPISQAKGYKSQALLADLWIPQPTPAPEAHLAVDRPPNHINTPLVTNTLTKGYKMQARKTLFSFVLVVSALAGLASAAPSSSTADAAAAAPPTQEKAGSPAAAASAASADKQAAAGQEGASTASQANPQNAAVFAATGTAEAPTPGATDGDTSGKAGHKKRNADAAPAGKIPDSADKADSATQANPQNAAVFAATGAADATTAATTGEDASGKADHKKRNADAAPTGKIPDSTDKADSTTQANPQNAAVFSAASPTDASTVSTAGDEASSKTGHAKRNADAAPTGKIPDSADKADSAAQGVPQFNAVDAAANGQATDAAFASTHAVAQTRRSEPSGAAADGKKDATAENTSQPSPKDSQTPQEAQQLNASPSGSTPDSGAENPASDKKDHWCVFLFHFPFGEYHHVFLSSSPGLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7D8</accession>
    <name>A0A5B0N7D8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007831</name>
        <name evidence="3" type="ORF">PGTUg99_001920</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000415">
        <property type="protein sequence ID" value="KAA1085191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7D8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="91" mass="9884" checksum="782FA7C3BAC51ED3" modified="2019-11-13" version="1">MPAPNVDIKVISDSAPQKPSLLAATPSQPSASEDPSSTPTEQMTTSKKRKTTSEIWEHCNTEGHSQDLKEICKYGLGEISARSTSGMNHLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NA93</accession>
    <name>A0A5B0NA93_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034754</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NA93"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="307" mass="33851" checksum="39F2A5815F4A94E4" modified="2019-11-13" version="1">MAMYWYQRCNRSDPRIGIYRGSDQNIPQYRVSGSDCFSDPIPIYQMIGISWGDPIRAVWELGIGIAQADPRFPRSCIVARPHYLGPGRSSFPTVYCYSNTPPHHPSSPTPSPTYSHHPITTPPHRSSASSPRLESDDRPPPSSLHPSIVGSDSDSNRDCPASPTRESLLKFPSLRFVCVESDDPPPLVASLFTRRRVGLGIDRTPPGLPHSPFPAPALRFVAGYLIWRPPTLVASPFKRWVVLGLERTPPGLRHLSFPACPPIRRCFAGRAAAAAAVVALGVVGSTWYPLPTTFFCFSRCARQLVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NC15</accession>
    <name>A0A5B0NC15_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA endonuclease activator Ctp1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_031917</name>
        <name evidence="9" type="ORF">PGTUg99_012359</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NC15"/>
    <dbReference type="OrthoDB" id="5801062at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010792">
        <property type="term" value="P:DNA double-strand break processing involved in repair via single-strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013882">
        <property type="entry name" value="Ctp1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033316">
        <property type="entry name" value="RBBP8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15107:SF0">
        <property type="entry name" value="DNA ENDONUCLEASE ACTIVATOR CTP1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15107">
        <property type="entry name" value="RETINOBLASTOMA BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08573">
        <property type="entry name" value="SAE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="697"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="737"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="DNA endonuclease activator Ctp1 C-terminal" evidence="7">
        <location>
            <begin position="627"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="407"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="618"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="358"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="383"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
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        <location>
            <begin position="449"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="490"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="521"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1072290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1086326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="770" mass="87039" checksum="CAF66A437733A87D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NC70</accession>
    <name>A0A5B0NC70_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NC70"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="411" mass="47280" checksum="D6D05C9EFA25DF30" modified="2019-11-13" version="1">MWAKMGQKAPPSPTKLPWATPEAFHSLVITIFAVIKASRTLKKLPSYHPAPKINLSERSQLSSFNFEDPQDPIGLLLVSPSVPVNLQENAELIQRYLVILRLRKEETERNYIPTPWEKLSEEEQLLLATYHAHIHKEKDLEEQIKFRCFLREIRSKNQQLRRKRYLEKMSTFETGVIRQERASPTPSVDTDNGSVSKDSDNLTAQNYEAPSQLQRDLVAAKELQSRVENLKMTTLVRKENLAMSQDAENSREEPPPSTEKSSAMEIDEIESECSEVTPKRPITPEIRVLTKEDQIKLRVKEHVSLWRQCQVATREGPTEKLQALLTTAQESQKALQKLIDNEEIESYVKGWNPWDEKKLHFPSPPKKNLTKFKRNEPGKKQSSSSTNFSDPAKWKKVTDLLQMASGLYQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDL4</accession>
    <name>A0A5B0NDL4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010501</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDL4"/>
    <dbReference type="OrthoDB" id="2507145at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="241" mass="27594" checksum="4B1F32EBEA41C384" modified="2019-11-13" version="1">MTRLKVRVDLTERQDPPELSLSRGPNQPWFLLCLRPRRHECYTTRNDSSTEKPLLDKEKLWDRICKAKAASDEGNADFLLRIYLALPKEGVAESLRVPVTLEELCSVSADASIPTQKSTINKTVKFIKGLVPNHCNIGFTPFFDKNIQEFKGPLPLTIFDKEWQGDAVNVHSGKKSKSDEKDGNYTGYKYPNEWTQNFSVWTNNFRSFLITYRDIYKINDFAECILAHKSNVDEIIATDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEM2</accession>
    <name>A0A5B0NEM2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020605</name>
        <name evidence="1" type="ORF">PGT21_020711</name>
        <name evidence="4" type="ORF">PGTUg99_020308</name>
        <name evidence="3" type="ORF">PGTUg99_020690</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000175">
        <property type="protein sequence ID" value="KAA1125793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000007">
        <property type="protein sequence ID" value="KAA1137561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEM2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="57" mass="6451" checksum="7BA95A226DFF27CC" modified="2019-11-13" version="1">MSEVQTYTDKNGAQITVCQPHQLEYCMRCQMDFTEMNEEARAEAAIAAGQEPDDGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFV4</accession>
    <name>A0A5B0NFV4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3365519at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="821"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="398"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="597"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="749"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1297" mass="145063" checksum="AB5D09090AACC830" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJC7</accession>
    <name>A0A5B0NJC7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJC7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11363" checksum="9302AD15C3646191" modified="2019-11-13" version="1">MSRIGAPRPVNFFRLQAHPQLKDRPRGSPSSHIVLANSRSAGQVEIGAKDHNRPSFARNAHPPRLLQYNHLQPPGRHHSASNLRASACLGFVCRSAMLQRSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKE0</accession>
    <name>A0A5B0NKE0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKE0"/>
    <dbReference type="OrthoDB" id="10358041at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="148"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="282" mass="29337" checksum="B5F26D8989811CAC" modified="2019-11-13" version="1">MDPSNSGVNGVPLRTLDFAAASSPSSLQMVTTRSKAQTPSCPPQRCLPGPSLASSASPAELHANKYMISGPVRPFTPVNSPATKVPVPIHPSTVALESSRAQEHVDPHLVQEGINFYPLQSLWSPAFTPSAGNLPPGLRSPNFIDSGSTDGPSATESSSSSISDGFSSGSESESSAPASAPPSIPNSSAFPSPSSSDLCSVPPSEIFYANRPRPQPVATGSGNSYDDPMVIDDNAAADSDPESLPFGPTTKWGSTQQARPPTNPVNPDPSAREMMEFDYNTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NL28</accession>
    <name>A0A5B0NL28_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UTP23 sensor motif region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_013468</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in rRNA-processing and ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UTP23/FCF1 family. UTP23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NL28"/>
    <dbReference type="OrthoDB" id="25675at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09865">
        <property type="entry name" value="PIN_ScUtp23p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057776">
        <property type="entry name" value="UTP23_sensor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24779">
        <property type="entry name" value="UTP23_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP23 sensor motif region" evidence="8">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="183"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1089302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="290" mass="32398" checksum="DDDB3C0DFDFFDEE4" modified="2019-11-13" version="1">MKTKRNKSNRKTMRVYNSIFKFREPYQVLFDADFAVTVTTQKMEVQSRLAAVLGGTVKPMITQCSIHHLVNLASDGSQVAQEAVDLARNLCERRKCKHGTIKASSIECIKGILGDTDNRLRYIVCTQDPHFRTYLREKIIGVPIIYINRSGTLILEEEGPATELRRKQLQEDKLHVPPEELEQLKSTDTGPDSGLPAQLIQGPSTTNPLQTQSSDTLSNGQTHSAHKLENKLLKKKKRRSGGPNPLSVKKKKSSASNRRRIPQEKSASKNTSTQKTSSKSSRKTPPGKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NT23</accession>
    <name>A0A5B0NT23_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017820</name>
        <name evidence="3" type="ORF">PGTUg99_010940</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000378">
        <property type="protein sequence ID" value="KAA1092063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NT23"/>
    <dbReference type="OrthoDB" id="10320957at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137550" evidence="1">
        <location>
            <begin position="26"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="607" mass="69859" checksum="1947FDA2C4B39BB4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NV41</accession>
    <name>A0A5B0NV41_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005474</name>
        <name evidence="3" type="ORF">PGTUg99_007641</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NV41"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="110" mass="11425" checksum="31608B87B559E9E5" modified="2019-11-13" version="1">MEEASLALQNDRPQPVCARCAHVNGSNSATRRGCQLGKPTKKAGPTRDASPRLGAVPNQASPRHRQPGHQVAHSSDSMNLGPQTNCITGTDSNSLQSETASPVNNAGAVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NW59</accession>
    <name>A0A5B0NW59_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">VMA13_2</name>
        <name evidence="9" type="synonym">VMA13_3</name>
        <name evidence="8" type="ORF">PGT21_017500</name>
        <name evidence="9" type="ORF">PGTUg99_031204</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase H subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NW59"/>
    <dbReference type="OrthoDB" id="10263554at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001537">
        <property type="entry name" value="V-type proton ATPase subunit H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.150">
        <property type="entry name" value="V-type ATPase, subunit H, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004908">
        <property type="entry name" value="ATPase_V1-cplx_hsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011987">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038497">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11698">
        <property type="entry name" value="V-ATPase_H_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03224">
        <property type="entry name" value="V-ATPase_H_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032184">
        <property type="entry name" value="ATPase_V1_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase V1 complex subunit H C-terminal" evidence="7">
        <location>
            <begin position="371"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR032184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1092896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1115770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="489" mass="55665" checksum="EE122F08F9B6FC1C" modified="2019-11-13" version="1">MRIRRDAYTEVELMTNDEVQPKIDFHPTSLLTINLTHQPSKLIMSKFFNAWLQDQDCRTRIREIPWEGYHRASLISADDLALLQATASQPKLKQEQIWEHDTEKYVSLYLNLLSQLSRVDCLQATLVYLSDMIEDHEDRIALLLDHAVNPYAALTPHLESPDYYIRIKALSLLSVLLEFHPTPPQDVLSTIGPMIARLTGSGDSNEQELGIELFEHVLRQPLARQAVWASAEESGASSNGETTQSNVISSLVQMMKSASRMTAQIQYQLAFCFWLLTFDVAIASQFNKRFSVIPLLTELARSAVKEKVIRILVATFRNLAEKAPEANLATMLVAGNLLPLIQNFSSRKWSDDELKDDIEWLKEQLEEAKRKMTTYDEYLTELESGLLRWSPPHTSEEFWSQNADKLNEKNHQPLKKVIELLSSASDPVVLAVAANDLSQYVKHSDVGKRSAERLGAKPVVMKLMTHQDSDVKYWALVSVQQLVSQPWSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0L7</accession>
    <name>A0A5B0P0L7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed DNA polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_035177</name>
        <name evidence="5" type="ORF">PGTUg99_008866</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="342531at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007015">
        <property type="entry name" value="DNA_pol_V/MYBBP1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213:SF2">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04931">
        <property type="entry name" value="DNA_pol_phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="811"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1245"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="814"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="840"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1292"/>
            <end position="1303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1095061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1121327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1310" mass="144724" checksum="145FD84F0D024546" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0W5</accession>
    <name>A0A5B0P0W5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_019567</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0W5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022839173" evidence="2">
        <location>
            <begin position="20"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="158" mass="16472" checksum="8ABDF7517CD7FD88" modified="2019-11-13" version="1">MHCSFFLAFLLAAVALAGADNGLVSLGGSEDRSGEGYPTRGPRRGPLADEESSRGDSYQDHMHQKREEDYGFNAPPGYGVGGRKTAGEFGSQYPQSRFPSKDGVRERKGYMGGDDGGSGYMGGGSGSGSGFGGGIALGSRMKDLQIGDLEAMSTDQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P240</accession>
    <name>A0A5B0P240_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P240"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022763702" evidence="1">
        <location>
            <begin position="18"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="182" mass="20311" checksum="308F18C08B1DD2BA" modified="2019-11-13" version="1">MSLVLNCVVTLVLPSWGLELRPYTGYSLRPGSASPFDYPLSNYRSSTRLSSSQRSSSLAYPPRLSTSRPVFSTIFSTIFTTIFTTMASSILQFFSNRPGIGRPPIKYVRGFKLSLASRIFLIDPLWIIQTDLNSHWPTSHELALHLNALVSAIRLDPLLVQSAGQLLARFIILTTSSMLLSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3M8</accession>
    <name>A0A5B0P3M8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3M8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1095080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="58" mass="6738" checksum="EF00E01266699DC7" modified="2019-11-13" version="1">MYQLQIVLDTANPYAHFYRRIRETLSGSGTPQYVLRTVESTAFNPKTYNKPTVEEVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3X3</accession>
    <name>A0A5B0P3X3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Threalose-6-phosphate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TPS2_8</name>
        <name evidence="3" type="synonym">TPS2_7</name>
        <name evidence="2" type="ORF">PGT21_036393</name>
        <name evidence="3" type="ORF">PGTUg99_026658</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3X3"/>
    <dbReference type="OrthoDB" id="2499136at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="349" mass="40131" checksum="1145DB1C30974107" modified="2019-11-13" version="1">MLLTTVTRALLVIKAWQHFLVISTPMCSLSTSLPRARHIDEVDIINKKTFREIFDGYSKSKRRLILLDHDGTIKPVCSSNAAEDYARVLRVLKALAGDFRNEVWIITASSLSGIKRLYGEIPNLNLAGRMGTQIRRATPIERKMELPHAEITKQLKCMATAISRNMGLGDPILLGDYSIKFEENSSKNEELKEMEHRLNKLIRERFVDYEGGCDYPDFGMSTIELRHKRLHNKGTLARLRLEETGPPFDFGMSIGDLKMDEDMHQEMRKKGLFAIVVKENDHQDIHHHLESQQISHQTPHGIDHTEDKHWETYASHRLKNFEKVITLLELLTSIKCSKGIFSKVKNLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P700</accession>
    <name>A0A5B0P700_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030215</name>
        <name evidence="4" type="ORF">PGTUg99_022706</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1108006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P700"/>
    <dbReference type="OrthoDB" id="2499284at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366391" evidence="2">
        <location>
            <begin position="24"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1108006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="811" mass="94375" checksum="51B8C687416B95D4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PDV4</accession>
    <name>A0A5B0PDV4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PDV4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="168" mass="18327" checksum="231F7CC3CFF44EB5" modified="2019-11-13" version="1">MPSDCAMKLAFSRQEARKPTFIPARAAYLTDFNGCLTHCLLSSGWLGRQEDITAYEAAFVLVCWASRSRIYLGTESLGIRIDLPGQSLQAVDTGSVLEGLVASLPGGSVPRACFKFVEVQRNDHCALFKSNLCYVPPVNHACSRLFPGRQGNSPRQELHPPSSSPGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PE15</accession>
    <name>A0A5B0PE15_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022818</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PE15"/>
    <dbReference type="OrthoDB" id="2503459at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="206" mass="23302" checksum="07242383A0744384" modified="2019-11-13" version="1">MEILSDPTETLGDLCNHRLADKSKLQPPRVKPSHHASAAKSKQKKKKKKNVVRTNNEDDSDQVGGAIDITQDSDHNNSKVKKVSKKNSEFDDILEYFEPPFHANKGDTGEKSSHRCKWCSGPYKKSKGTNSNLTKHRDGTKERPACSGRSDAIAFGAKLPLTAMEIEFNKQNHKEDLIHNYAQNTVFDMRVLNQLLVMWLIRYSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFY2</accession>
    <name>A0A5B0PFY2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFY2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7675" checksum="224700FA52424223" modified="2019-11-13" version="1">MASSTRKRKKPNSSHPSSPSPSRSQSQQRNTTSTQENQSDQTKGEDNDQPTSTAPIETTDEEELTWLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIV5</accession>
    <name>A0A5B0PIV5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007714</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIV5"/>
    <dbReference type="OrthoDB" id="2564904at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023100293" evidence="2">
        <location>
            <begin position="24"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="277" mass="30625" checksum="8553120EA2BC7778" modified="2019-11-13" version="1">MNHYCVKYLCLFLSLALPQASFALLTGFNYKNLPYQSDGAAGQTGYNQCGTKAHPNSHCQNAFIKSAEDFCIFAPPRPLVVGEAERIAVSYCSKDGYGTRLIPPGTFKNLHFVRTPHYVQITALGNFTKVNVPKGDEGGELDPSGADNHGNPVGGLVFGERGQFNHWTEFIAYDEFCIRACFNTPESPRYCQHIYDLEGCAWNIPGNYDGPGFDECEGEDVPLPMGEYRRPDGSIYTWQRGSAPQPPPGAPGKLKKCKSVHAPGLNYPRSKRFERNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJ94</accession>
    <name>A0A5B0PJ94_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJ94"/>
    <dbReference type="OrthoDB" id="2496520at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="405" mass="46699" checksum="1DC386EC5CD8824C" modified="2019-11-13" version="1">MENLSTNTPSGSGPQLQYQRELVINGFTNLERKYSSERTSIEEDPWSFPFNHHGMNSREVLYDRLFSTLLPRLKDQITAISATLDPVNMKTDPVAKLKRVLEIQSELDYNITQIDCAFLVICPENVSADDHTDDQGLEHFKAYRLVELKNEFTFALKEICRFFRVTSMYIQQMRPKLRGFFGDINISNCGKSAKDAVYSIEHTIKHPRRSELDIVANKAKRAVCLLDERLIDFISLVDPDAPPGYLLHRRFDIMTHQPLIHLAKLTIPIMKLSNLFFKKLSQLRMNCQAPPLFTQMNSDQLKSLCESTESVVRNLSGLMHEFYEVDEMFEDQAVDFRSFTQSVEQITTCLEGPLLSVLLYLVPLTNNPRDENYYQSWFAAWNTTINTAIRKFVQAAKNLQPNHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJK5</accession>
    <name>A0A5B0PJK5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJK5"/>
    <dbReference type="OrthoDB" id="2500493at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="216"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="639" mass="70291" checksum="CE3115FFCB71BEA2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PK22</accession>
    <name>A0A5B0PK22_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_018994</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PK22"/>
    <dbReference type="OrthoDB" id="3220443at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031101">
        <property type="entry name" value="Ctr9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027:SF2">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="65"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1101406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="12772" checksum="5C091AE94B3A15F2" modified="2019-11-13" version="1">MEVYMLCNLGHCHYLRDEFDRAIENYFTASKRFYDDQNVLVLLYLARAWYQKASKDRSFAALRSKRPSSERWSDCVQSSPGAAKGIGVASSSGGFPPDELDYGPSGFKRSESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKF0</accession>
    <name>A0A5B0PKF0_PUCGR</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">PGT21_035752</name>
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    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Schwessinger B."/>
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                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <begin position="282"/>
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        </location>
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            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="416" mass="46482" checksum="64266F9869259678" modified="2019-11-13" version="1">MMAGRFIIFGLLGFLFIIASNAIDDLVPERFSSLGNVDLDDTINFNALNQYLAQQTPKDGPRAPNSNPSTNTQEAAPNAGRPSGQLDSEAQIGRIGEARLARANDIQFLPAIQSHPAKFSTEAVLTPGMTYPVVPRTHQTLNQDPRRADANAQNPIKKLLLEYSQLSRMNKETPGMNVIDHNTQIGNSHDPRHLELLKSSLKPIPVRKDGRTAARLTSLTRPPLENQVSTSTSNRGFLNHPAYYDIYNAKHNMVELYQAGLDAYAEALEAAKKDEFIETIPTSTTSSSKISQNNDPDHRHPSEIALPTLSFDREIFTHEGASGRQKKGLALFKKLIEEHPENEDRLVVREADLSRVQSLFSKAKIYVEEGNNKPAEVRDANQMNVRRYSFTKEKNLEIISKQDLCGHTQARRPNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKI4</accession>
    <name>A0A5B0PKI4_PUCGR</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Miller M.E."/>
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                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    <dbReference type="OrthoDB" id="2505208at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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            <begin position="98"/>
            <end position="118"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
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            <begin position="126"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="213" mass="22831" checksum="42971977C0C4D571" modified="2019-11-13" version="1">MTTNQQQLPRTSSFNHLFGGAILDGLKLSSIVIPGGLLIHQLRQHMKLSQSLSTSAHTTASAPPPPSSSSTFSARSSPTSKINSLNYRLTQFLKWNGLLTFGFSLLYAAVLTTSSSLIQHNIKPLSTTTTRNDKPKSQRSSHSNDYSVIGGTLGGLITTTIFWNRRLFSLLSIIPGGIGLGVGAGLSTAYLHSLQSRSPNQPSSIADPEQQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKK0</accession>
    <name>A0A5B0PKK0_PUCGR</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Silverstein K.A.T."/>
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                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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            <end position="377"/>
        </location>
    </feature>
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        <location>
            <begin position="378"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1102095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="549" mass="61548" checksum="E787FAD9C74741A8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMX7</accession>
    <name>A0A5B0PMX7_PUCGR</name>
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        </submittedName>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OMA" id="ICAICAR"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
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            <begin position="483"/>
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            <begin position="519"/>
            <end position="630"/>
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            <begin position="324"/>
            <end position="359"/>
        </location>
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            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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            <begin position="57"/>
            <end position="70"/>
        </location>
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            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="525"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="560"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="645" mass="71643" checksum="159DF6C48B44F5AF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQI7</accession>
    <name>A0A5B0PQI7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tethering factor for nuclear proteasome STS1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">PGT21_002400</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in ubiquitin-mediated protein degradation. Regulatory factor in the ubiquitin/proteasome pathway that controls the turnover of proteasome substrates. Targets proteasomes to the nucleus and facilitates the degradation of nuclear proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Binds the proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the cut8/STS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1102972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQI7"/>
    <dbReference type="OrthoDB" id="10061064at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031144">
        <property type="term" value="P:proteasome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1590">
        <property type="entry name" value="Tethering factor for nuclear proteasome Cut8/Sts1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013868">
        <property type="entry name" value="Cut8/Sts1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038422">
        <property type="entry name" value="Cut8/Sts1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28032">
        <property type="entry name" value="FI02826P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28032:SF1">
        <property type="entry name" value="FI02826P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08559">
        <property type="entry name" value="Cut8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="18"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="98"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="395"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1102972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="460" mass="51127" checksum="A5B23775BDF398C5" modified="2019-11-13" version="1">MGLVQISSASCLIGTSKSFLSSRSPSSSSPSSPSLSLHTTTISPKNQKRKPKPNQSKTKQRMSTTYPPVIPITMTHQVPFRQPAITVPLNWSFAGAQQPNNNINNSNSINSLRKEQPQTNNSSFNKSRKRSASNEFNPAEQPTTSPLKSISSLPKRTKRTLPNSNPSLDADHGLNPKEDPTDLGTALVSLTKNELISLLHGLIQQKPELKPMIKSLLPPPSLESILLKLDEVEKQLVESIPNKRMNREEYIWNRVRVPLNSYVNDCLSCLELAAQTIENRCGSRAEGKPELSVIYGAVSDQLSLLSHLSLSISKVESLLPSLSTQPSNQNDHQDLNWNVSYPFQSILIPSLFQNWKNFIESIHKLIFIDRMIISESLIRKWFSLLDGLSSPNPISTTTTTTSSSSSSSTNHQLDSHQSIFGTHPHPQNTDQHDNHTVQRLMIAVRDEALNRFGVLVGLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PR53</accession>
    <name>A0A5B0PR53_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PR53"/>
    <dbReference type="OrthoDB" id="15235at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="106" mass="11331" checksum="3150E7F3662BD345" modified="2019-11-13" version="1">MDCHSGGQPPSLLIPNPVLVNLALKLLGADGVSDDFAWSSQHDSLYPKVQLALQQVTAPKGSLIVIANEGDTGLSLDRRRLHSDLEGPSNGRLLARSLDYHSYAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSZ2</accession>
    <name>A0A5B0PSZ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_022245</name>
        <name evidence="3" type="ORF">PGTUg99_029485</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSZ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366467" evidence="2">
        <location>
            <begin position="22"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="111" mass="12138" checksum="2B487F7116315BA8" modified="2019-11-13" version="1">MFKTLFQLLLLLLVGKQFAMGSFTALRTGKLKLSSNDKTKDVDAMDFKTSGGDHQLQPESPHALVLPKDPRDRMVIMHGTPSSEKSAAPHPRSTVEATEMAVEKAMSDALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTE4</accession>
    <name>A0A5B0PTE4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTE4"/>
    <dbReference type="OrthoDB" id="1726263at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="290" mass="31117" checksum="DA9F48F491FA6318" modified="2019-11-13" version="1">MFFCLPRRPGGFPPGRRYNLAGPGGNPPGRRGGLPPGRRGCTTSPAQEGILLVDGEDSLLVDEAVQPRRPRREHSWSTATRLYNLADQEDSLLVDGTTSPTQEGILLAGKVVAGVVAGAHLGQSFGRSHPGPPQVLPRSSPSQVPIDLSRAQQALPYEPILFPKLRIYFADFPYLHYKLPRTTNRSKTCCGAEKDKKSPRARLSFHTGQEGLVGEAVQPRQPGGFPPGPARLYRRPGGNPPGRQGCTTSPAQEGILLAGKVVAGQEGLVGEAVQPHRPGGFPPGPARWYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTX9</accession>
    <name>A0A5B0PTX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2590746at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF12">
        <property type="entry name" value="AT HOOK TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <dbReference type="Proteomes" id="UP000324748">
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    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
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        </location>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EMBL" id="KAA1105562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="682" mass="75408" checksum="BFD113F074832BD3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXS4</accession>
    <name>A0A5B0PXS4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TdcF protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014458</name>
        <name evidence="2" type="ORF">PGTUg99_019606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000345">
        <property type="protein sequence ID" value="KAA1098881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXS4"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000001">
        <property type="entry name" value="L-PSP family endoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019897">
        <property type="entry name" value="RidA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="21550" checksum="C767BD1F57EC6183" modified="2019-11-13" version="1">MVKSNPGLSAVRRAVFQNLNAPLRRYQALVIGRPGPPSSIFDKPTYPSRTPYRSLSNTRPLWNTMSAPISLNTINGTPGAPEAIGPYSQAVVTSNGLIYSSGSIPLDPSTMKVIHPTDVELQTEQCLKNLSKVLEQAGGSIQSVIKVTVFIKDMAQFSKINEVYAKFFKDHKPARSCVEVARLPLDVLVEIEAVAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZ32</accession>
    <name>A0A5B0PZ32_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA repair protein rhp7 treble clef domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030745</name>
        <name evidence="4" type="ORF">PGTUg99_022487</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZ32"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056451">
        <property type="entry name" value="Znf_Tbcl_Rhp7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF95">
        <property type="entry name" value="F-BOX PROTEIN YLR352W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23550">
        <property type="entry name" value="zf_Tbcl_Rhp7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair protein rhp7 treble clef" evidence="2">
        <location>
            <begin position="171"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1106191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1109241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="669" mass="73985" checksum="7EF0B930BE64D34D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2L2</accession>
    <name>A0A5B0Q2L2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Osmotin, thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010923</name>
        <name evidence="4" type="ORF">PGTUg99_035830</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2L2"/>
    <dbReference type="OrthoDB" id="430315at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31013">
        <property type="entry name" value="THAUMATIN FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31013:SF2">
        <property type="entry name" value="THAUMATIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137885" description="Osmotin, thaumatin-like protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="82"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1124839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="19968" checksum="F120C8E72721C221" modified="2019-11-13" version="1">MLKAAVIIALLSSSILAVVVNGYTLHFKNNCKFPVWPAVGKAPNGQPDPSVSYGTKLNPGGESQFGVNDREIGIRSWGRTGCDPKGANCATGACRGGLRCNDGGITSRVLLGEYGYQSFGNVISWDLSRVDGSINIDTSINNGGNAKTCRKNNCPSNQAFAKPNDFQAITTSPTRVRLSYPLDRPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2V4</accession>
    <name>A0A5B0Q2V4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_014601</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2V4"/>
    <dbReference type="OrthoDB" id="2506775at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1107457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41648" checksum="F4292B688AC0E108" modified="2019-11-13" version="1">MPKITEENEDGNEGDDEDSDSNSEAGSDHTEEDDAESDDDDEDDTDESQEANKGAKDGQKESSSKANRNNLNDLNDLVTALDFVVKKITGSCAWRQEFERRAAGKGLKNLIAGYGIRWNIIYESRTRAYEAREVIDAILHDEYDKYKQQRSKSSRKENPKKLGHFKEIQFTKKEWAMINELNEELEPFNRLTKLMEGDGPTGAFILPNYYKIISELKNKEDACNRGHPMHPMYVKMIKKLETYEKEALECHTLIMATLLHPKLRLKAFTHCWPEKADFGRKLLEKEFAKRVEDLKKQKEDNIQIVEKEAPPVEQNDIFEMFNAPARTDESKELEVYINNLDRLPLPASNDPKSILIWWKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4Q9</accession>
    <name>A0A5B0Q4Q9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004831</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4Q9"/>
    <dbReference type="OrthoDB" id="413993at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="81" mass="9330" checksum="6E6E30B8316F88A4" modified="2019-11-13" version="1">MGSENPTLQADSEGDKYTTQEHGINLCWHSPNILVEVLQQTFEDCLKRPTFRTRASSQCSLTHERYPMRSIPTHANRNELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7J8</accession>
    <name>A0A5B0Q7J8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_033424</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q7J8"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17919">
        <property type="entry name" value="DEXHc_Snf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000114">
        <property type="entry name" value="DNA repair protein rad8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003221">
        <property type="entry name" value="Nucleosome remodeling complex ATPase subunit (Snf2h)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="161"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="527"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1109121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1043" mass="117957" checksum="CCD43764F5106D7C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9U6</accession>
    <name>A0A5B0Q9U6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9U6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022688022" evidence="2">
        <location>
            <begin position="21"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="225" mass="21211" checksum="D77FA967293B6D46" modified="2019-11-13" version="1">MLFSTKVVVFMAALAAQASASTSKPAPGAMDMSTPTPGSGDSSSAPPADSSANSTAPASPAGGNNSTSAAPSTGAAAPANSSSPSSGSAVSPTSAGAPVPGDMSSGQCMCPPVPQCGANPGSPGLPSPGSSAPSPSPAGPAGQQTPVTPAPGAPANGTNGAVSPVGTTPGAGAGQTTPPENTNTTVSSDTFAMNCSTAIDPLFLYLTRREAAVLLFATPSRLLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAW9</accession>
    <name>A0A5B0QAW9_PUCGR</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAW9"/>
    <dbReference type="OrthoDB" id="10538046at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="8073" checksum="12C0A34AEEC636F8" modified="2019-11-13" version="1">MYGSPGGTPIEATLGCYAKRPTNVGVAALKTARQTCSVSATITTNNTDFAPRKNGNLDGGNRSIFWDLFYKMPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC25</accession>
    <name>A0A5B0QC25_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peroxisomal membrane protein PMP27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PEX11_2</name>
        <name evidence="5" type="ORF">PGT21_029309</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="411017at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="InterPro" id="IPR008733">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
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    </dbReference>
    <dbReference type="Pfam" id="PF05648">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1110675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="249" mass="27965" checksum="7AC1ECA8051B79BF" modified="2019-11-13" version="1">MASIANQVIFHPVISSSLKVGNTTVGRDKLYRTIQYFSRFLVWYFEKNGYDKQTIMRFNNLKSALGVSRKLMRLGKPLEHLQAALKVTKEISDPVVGVCAVGRQISYAIYLFNDMMIWAEKIKFISLDKVELALVNKRAAQFWMAGISLNVISGIYKHQQVQSKLRRLKRANKSFGEHEKEEKKYQIQSISAQAFAVRLQLLQDFCDILSPSSTLGYHSLNDGVIGGAGVISSLLGLRSQVNKVLGSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHZ4</accession>
    <name>A0A5B0QHZ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHZ4"/>
    <dbReference type="OrthoDB" id="2495337at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1076" mass="121139" checksum="B9EE5634437842F9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QI83</accession>
    <name>A0A5B0QI83_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Y box binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">YBX1_2</name>
        <name evidence="2" type="ORF">PGT21_008588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QI83"/>
    <dbReference type="OrthoDB" id="422005at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="254" mass="25125" checksum="C2FC8960CA4AB765" modified="2019-11-13" version="1">MRSSKFQVEYVVAPGQKGFQATEVTGPAGRAVQGDVKSKMPKTPTFVPYPMGIMPAAGAYALAGAGSPYMQPDPYMAAGAYPAPYPGQVFYIPSPMGVIAGGHSPNLTAMSPYAGATSPHPSTRSPTIPFTHHQHGQVAMALSPQPAAGGFGFGSPSMAYGFGGSSGSAGLPGASPGAGSGVGEMRSPRIVSAGGGGSRGLAGSSHFGAEPIHPRAGPVGSVGGQNQLRGFSNGGGSSGSLLYTTGSGVPPSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJJ5</accession>
    <name>A0A5B0QJJ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJJ5"/>
    <dbReference type="OrthoDB" id="10278045at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="369" mass="41399" checksum="560B9C90C84D8A3C" modified="2019-11-13" version="1">MAEKTDVHPLKGIDYSEKDIGVLVRQLCKNLNQQHSQCLTEVVDFDLQAVFLMQEIGPHHRIRSIVKMVLKQFDINTQPPKTPKKRSPSSAPPQNMLQQQLLDAAKQVQKTIDTFDNNTSSRKPFCLQFPSYKHVLQNAISMQDKCQKLPTNRMVVLLDEAGICVGVVVPPFPKTPGKHHPEADDCACQALNKTVLTSSWNTNEDEVQYSSTPLDPHPRSPFPLGKSNKGESRTPKGGGDTKTSMSFQTYGFGLGDTKSKGMNDLKIPILKNQINTAKLQGYSNSWKNVPFTPSLPNSLRKSQNIQDTQARVHLRNEIKFYSKLSLWINKSFLPASTKITEQGVQYLMSSDSDLLRKNLAKENNQIIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN32</accession>
    <name>A0A5B0QN32_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN32"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="162" mass="17679" checksum="A3EF8C4CD9F883E0" modified="2019-11-13" version="1">MANLFDPQFFQPGKAANTCDSQFFQPARAFTKEKLLEGSPSSDKSSSSTTRSQDKEFIPKTATKFGGVIRIGPGLWNSVYRLSSRGVEFCPEHEASTMPALSSEQTAGVRLLEQPELILALGSAAKSVGPLYKGLTWIQTAQYIGEFENICDSTATCQKTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP37</accession>
    <name>A0A5B0QP37_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP37"/>
    <dbReference type="OrthoDB" id="10684981at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF22">
        <property type="entry name" value="TRANSPOSON TY3-I GAG POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="253"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="767" mass="84181" checksum="4FB4DC4A98BA29D7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSQ4</accession>
    <name>A0A5B0QSQ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSQ4"/>
    <dbReference type="OrthoDB" id="2510764at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="462"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="560" mass="62922" checksum="8B5B2D53679C04A6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSQ5</accession>
    <name>A0A5B0QSQ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007666</name>
        <name evidence="2" type="ORF">PGTUg99_029336</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000474">
        <property type="protein sequence ID" value="KAA1073991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSQ5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366518" evidence="1">
        <location>
            <begin position="20"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="91" mass="9694" checksum="FD3630C321EA86FB" modified="2019-11-13" version="1">MQSLTFLALLLSASTRTASVGPQQEQESLTKEPTFFDMLARWNGDGYSGGYVGGGYRGGAFGQTTFVDTHLTPSSPAVFATEKSPIPRSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTI9</accession>
    <name>A0A5B0QTI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTI9"/>
    <dbReference type="OrthoDB" id="2506732at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="262" mass="29852" checksum="661FAA68BFFA294D" modified="2019-11-13" version="1">MLDKYNKLGREFIAANPGRPGPRSLEYNDLLELQPDDTFWNDGLFTNGSEPWAIDTLTQRGIRRLASLQRGQEEVRRLGWEVRRSMRWATQRHERLLLLFGELEEYPTDNPMVPPALQSLLGHRYLSAHTNLAEKWDSATLIVHSSFLEISELQLDWDSRLPKLFQKTTPQDGDDTLISVWAQQVTRIKRAVDHGLLSQVPGDMTSELLFVLYGGHPESLPMAFGDSGDEEEDNEESYLADIENILTETMQADLVQESGAND</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX75</accession>
    <name>A0A5B0QX75_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QX75"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="244" mass="26888" checksum="AB952258D6568083" modified="2019-11-13" version="1">MHTARTFLRFVHSLNDFKPLPPAFPPFKRLSLEPITQSELYIAPIKSLPSISSSNRFRYIRFVLRTPLSTLHLLLLVSHVQFVTKSILLIASSTYQDRCKIYLPLKSYQSITVAMTRTKQKHLLRALKRDGPLPLGGPDRLTMGNSINRQGHKGACAVVQPALKTATCIDGRSESPLSLGNTDLLARRKANNHQGYKETCVVSLPINKDASLPATSPLPTSSPDILEKCCENSFGNGGSPARGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXI9</accession>
    <name>A0A5B0QXI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026286</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXI9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="155" mass="17740" checksum="CA02671B2BF6F1B3" modified="2019-11-13" version="1">MPLDRFDCIIELAKLSIPIIKLFKLFFKKLSREGMNNKKSLKLPLFTQMNSNQIESLSQSAGKISGDLSELVRLLTQADLTLAREPNTIDNRPIIKIAGRLPTHFDGPLLSIVLYIVPLIDPLSDQDYYHTWFVTWNILINSAIHNFLQLARTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QY35</accession>
    <name>A0A5B0QY35_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QY35"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1118196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="90" mass="10557" checksum="9D994FA4B3761777" modified="2019-11-13" version="1">MYLALCRTWLTFRLNARCRRRTRANFLPPHHYQAVSIDNFFCTKSHSKQTLSPATHSQLWRPWRLTIVGVQTSSSGRSGLLRSYRLHVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZM9</accession>
    <name>A0A5B0QZM9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cep57 centrosome microtubule-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_033809</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZM9"/>
    <dbReference type="OrthoDB" id="76453at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024957">
        <property type="entry name" value="Cep57_MT-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06657">
        <property type="entry name" value="Cep57_MT_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cep57 centrosome microtubule-binding" evidence="5">
        <location>
            <begin position="841"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="221"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="279"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="609"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="716"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="807"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="611"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="660"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="722"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="807"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1118234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="933" mass="102826" checksum="2B08B8CC49416A36" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0Q2</accession>
    <name>A0A5B0R0Q2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015700</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0Q2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="13590" checksum="A8D0A899026CBCD6" modified="2019-11-13" version="1">MERTREMGIPHRGFRDLSCLAADKRVESVSKNQGVSFSFESSSLKLETQEWNLRCDRSDRAQKLFPITELRDHHRWAVLASRSLATHRLIMFRGRRKICDYTPLWSTGGDHLFPGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2R8</accession>
    <name>A0A5B0R2R8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2R8"/>
    <dbReference type="OrthoDB" id="10359721at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="512"/>
            <end position="537"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="567"/>
            <end position="589"/>
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            <begin position="1"/>
            <end position="22"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="36"/>
            <end position="45"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="623" mass="68062" checksum="4556D6D007CF7DC9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RFY0</accession>
    <name>A0A5B0RFY0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018704</name>
        <name evidence="3" type="ORF">PGTUg99_024416</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RFY0"/>
    <dbReference type="OrthoDB" id="2147986at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="201" mass="22990" checksum="071D55B90D4F3DD4" modified="2019-11-13" version="1">MLAKDIRLPLDNVPERTNPRFLSFAELQAIVAEQNKRIDSVAEEIKSMQQKDHKERMRVEALMEQVEAQMEQVNLLRKQAQMDAIHDFFRSIESTLCRRLGIEEDSCIDTLTKALKSGPTTWAELKTILNLDEKEDEEWLLATTNGIKEETLAKCAHASNTSDEQLVLSTSLVNLAQNNFSLTPPQLDILQKLVDWVRVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJS6</accession>
    <name>A0A5B0RJS6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008296</name>
        <name evidence="4" type="ORF">PGTUg99_010317</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000181">
        <property type="protein sequence ID" value="KAA1125235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJS6"/>
    <dbReference type="OrthoDB" id="10289663at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366614" evidence="2">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1125235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="81" mass="8446" checksum="C3C1A4B3EF578514" modified="2019-11-13" version="1">MPTRTALLYLILSVSALVGLGSAAPSSTQDALDHSHAIMQAKRNELAVSDKNLIKSRDASSVANSGNPDNLPGRDPPKPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RPV9</accession>
    <name>A0A5B0RPV9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028560</name>
        <name evidence="3" type="ORF">PGTUg99_008532</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000146">
        <property type="protein sequence ID" value="KAA1127961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RPV9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1127961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="76" mass="8415" checksum="A8D1B7BFA084C2CD" modified="2019-11-13" version="1">MLKKPTNNNNVVKKTYQGKKKHQGFHQSIVAIQSKGKAEEEEPSHHSNRSSDVPIVSHPAKQTTPINSASSNRLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RQW9</accession>
    <name>A0A5B0RQW9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028051</name>
        <name evidence="4" type="ORF">PGTUg99_016883</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000142">
        <property type="protein sequence ID" value="KAA1128376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RQW9"/>
    <dbReference type="OrthoDB" id="2502428at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039228">
        <property type="entry name" value="SZRD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31796">
        <property type="entry name" value="SUZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31796:SF2">
        <property type="entry name" value="SUZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12752">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUZ" evidence="2">
        <location>
            <begin position="77"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1066304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1128376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="216" mass="23268" checksum="E3633DE000DE0E3E" modified="2019-11-13" version="1">MPASKKKKADPCDDWEEQDYDSPTNGSVPSPTSDRPSPSNALPSNHQPVSSSSNIHSDAPPDPIEIENSLVWNNANRNPQYVILPANAAHSAVTANRPLVQETMFGKAKVTILKRPKAESTNRSSNNSNSSSSSNLSISLREKAYSEARERIFGSSASSSDDQSPSKPPIKLKPTPPRISRDPSSQANPVAIQRQPNGPASSDQKGFNSRQKSIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RUF4</accession>
    <name>A0A5B0RUF4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010630</name>
        <name evidence="4" type="ORF">PGTUg99_006388</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000137">
        <property type="protein sequence ID" value="KAA1129187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RUF4"/>
    <dbReference type="OrthoDB" id="10381922at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138203" evidence="2">
        <location>
            <begin position="23"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1129187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="309" mass="32425" checksum="C314C5C7F05D781F" modified="2019-11-13" version="1">MANSRPSPFILIQHTLILLLSCQQILVSVTSAPLSVSLGVAKAGSGLRDASFPAFHAGAGAASPLRRRAMLASTGRGEFEAMSQAANMRLTTQSLQAPTQFQRSASSSIRMPSAIPNPYLGRIPSGRKSVGTGAFIPGNPNFPKLQNPIGGSNRVSTGALAHPNPLPFESSTPKIAAQQASSRVPARNLQAAKQSQSNQRTMSAGRNPYIPRQSPLGWPRPSPPPPGPISKPAGFRPEESVTLSTASGRKANDRFSPPSNSRMSADSSPPPVWEQLAGVDQHVANTITQISHNRAGPTSLQRGGSNVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RX79</accession>
    <name>A0A5B0RX79_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Saccharopine dehydrogenase NADP binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_032283</name>
        <name evidence="3" type="ORF">PGTUg99_004872</name>
        <name evidence="5" type="ORF">PGTUg99_006291</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000476">
        <property type="protein sequence ID" value="KAA1072787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000111">
        <property type="protein sequence ID" value="KAA1130207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RX79"/>
    <dbReference type="OrthoDB" id="10268090at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000413">
        <property type="entry name" value="Trans-acting enoyl reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="2">
        <location>
            <begin position="10"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1081255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1130207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="424" mass="46323" checksum="670D18F73C891ACC" modified="2019-11-13" version="1">MLHTDRNIDIIVYGATGFTGKLVCQHLQRRYLDPSAEEKLNWAIGGRSQEKLDKVKSELGLPKDLKVFVADSNDEKALNEMCKQSKSLVTLVGPYAMYGDKLVAACVANGTHYFDLTGETLWASRQISKLESQARESKTIVVHSCGYDSVPSDLNAMLAVRELKKVAGQDVKVGRVTAGATAKGGVSGGTIASMLNMYDEGVSQLRKAMSCYLLSPVKGHQKKDPSWVTKEPGLVGGFFIMAPHNGAIVRRSWGLLEASSDSAIKNERYGAKFNYDEYLITPGQVAGFLLTLFFRVFALMMLVPQARSLVKKYGTQPGDGPDEITRKNGHFKHITTAHSVEDDVQVRVTMSGNLDPGYGWTAISIAECAVTCVRAHNKLPPLAHQGGFLTPSTALGDALLERLELTGTTKIDKQVLRNDRKKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S161</accession>
    <name>A0A5B0S161_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004863</name>
        <name evidence="2" type="ORF">PGTUg99_013101</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000109">
        <property type="protein sequence ID" value="KAA1130404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S161"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1130404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="191" mass="20925" checksum="331F41D90657A825" modified="2019-11-13" version="1">MNSRLGRSSFPTDPWSGLATYHTQVLKLSVLLPPTPPPCLQLNNSPPPPPSCRCRRLNNPQAPLLSQSLVRHLESESTLLRLSLPGPLSRLALLSCQFAYHPATSSQKSNGTVLVYCPSLLQDHTNPVSRSAAGRLPAHLSLVVGLNQLFKWYRVPPPLLQLRGQTNPARRVAWSAHSISSPNQVRLLSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SL47</accession>
    <name>A0A5B0SL47_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010989</name>
        <name evidence="3" type="ORF">PGTUg99_033368</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000002">
        <property type="protein sequence ID" value="KAA1138658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SL47"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366755" description="TNFR-Cys domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1138658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11944" checksum="A12A3E108E1BFE6F" modified="2019-11-13" version="1">MQIWKGTILCLMALLCGLAFAANLEHTASCPNPQTMWRTAGNLVACPKAGCQERYRERAEAHCPNCAAPTIIEVFNRGCDEHQDEYTTMNRAFRLYFRRKSFKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLC0</accession>
    <name>A0A5B0LLC0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016500</name>
        <name evidence="4" type="ORF">PGTUg99_032021</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000506">
        <property type="protein sequence ID" value="KAA1068301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLC0"/>
    <dbReference type="OrthoDB" id="2499914at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366010" evidence="2">
        <location>
            <begin position="27"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1064846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1068301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="298" mass="33143" checksum="814087E10AAD1EDE" modified="2019-11-13" version="1">MTTRSASTFSLLLLMLSLSSPKPCLSMEVYLEFEHQWNNPIVEKLVIPPTKAAATQKDNLSIPSASRTSVRSRKGKIPIAPCSSGENGSSWGKSIITTDADASTSSAHQLPSREERLQIALTKAFGSEHLIRIREAGFFDLAPRLTNFAGHKILGDLSDENSKEVRSIGNSIRDECKGGPIIRIRPFHKLPDTFDVSNALDIQHYGQQLSKWLEQTEYAWIEKLSAAESVSNENRDTMVSQLVEKSTQVKNHLDRLKEILGHEPEAFSTAFANRQAYLEEFLSDELTNSPFENGIQNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNA0</accession>
    <name>A0A5B0LNA0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029864</name>
        <name evidence="3" type="ORF">PGTUg99_031979</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNA0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137002" evidence="1">
        <location>
            <begin position="20"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="69" mass="7602" checksum="D1DF4EC284C56846" modified="2019-11-13" version="1">MKCFTLSAALVMLLGFTCAMHTRPVTQVTQVRAPTSDVETKLEEKVCIWCCDDDADHTSDQCGNQPGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPG4</accession>
    <name>A0A5B0LPG4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BCS1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPG4"/>
    <dbReference type="OrthoDB" id="10251412at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014851">
        <property type="entry name" value="BCS1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08740">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01024">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BCS1 N-terminal" evidence="1">
        <location>
            <begin position="43"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="194" mass="21822" checksum="9A009C66DB5E42B3" modified="2019-11-13" version="1">MTTQDNLSGAPSEELMPKTAGTDSMLDSILSQNPYFSAGFGLMGVGVALSGLKRSTTLLFNLAQRRLLVTLEISSKDRSYLWFLNWMSKQSRKNSSTNQLAAETSYRQLSDGTHQVDFALVPGPGNHFFKFQRAWFQVKRERDGKLIDLNSGTPWETLKLTTLSRDRKLLVELLKEAKSVSMKTEEGYQGEFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSD7</accession>
    <name>A0A5B0LSD7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025140</name>
        <name evidence="2" type="ORF">PGTUg99_025492</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSD7"/>
    <dbReference type="OrthoDB" id="2502642at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="513" mass="57970" checksum="E62662C8830A134A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LTZ3</accession>
    <name>A0A5B0LTZ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clr5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010209</name>
        <name evidence="2" type="ORF">PGTUg99_029831</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LTZ3"/>
    <dbReference type="OrthoDB" id="5392716at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21671" checksum="EF1C3874DB4C1315" modified="2019-11-13" version="1">MSDVYTSDYSTEFTGSDDTDHDEPTSSQNPQPTDMEQVVVRYLLSQGYKGPQILVILEERHGIKMSIQTLNRKRKLWGLQQRDLPRGPPPAIQASIRSSHSKGLNLKEIQARLYKETELDVSIRTIQRYLQQLNLRQQTNDLQDGKVSWDKVIECINHARTELLQTSAGYRSMHRILKRFYSISIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LV77</accession>
    <name>A0A5B0LV77_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LV77"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10626" checksum="4983B976A6D6DE57" modified="2019-11-13" version="1">MEVMPANERNPIPSARLSTLNLGDRIWICCHSFNQNQYGVLFGVTTIIFGLPNVSPMFLRARALSALSSRCEFIISITMECEEGILYFNVDGPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVE8</accession>
    <name>A0A5B0LVE8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mini-chromosome maintenance complex-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_026497</name>
        <name evidence="3" type="ORF">PGTUg99_013954</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVE8"/>
    <dbReference type="OrthoDB" id="329666at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019140">
        <property type="entry name" value="MCM_complex-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13489">
        <property type="entry name" value="MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13489:SF0">
        <property type="entry name" value="MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09739">
        <property type="entry name" value="MCM_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="62946" checksum="DBF3C75B584E7E29" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYS0</accession>
    <name>A0A5B0LYS0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYS0"/>
    <dbReference type="OrthoDB" id="2506775at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="773" mass="87967" checksum="1329D557C7806654" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZQ4</accession>
    <name>A0A5B0LZQ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZQ4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="335" mass="35305" checksum="0C9D34F35996E4CD" modified="2019-11-13" version="1">MPPNSCSADQDVFPNGAAADSQPDDNSKPNEAATDIDPNNHIALPDLGEEGLLRPAANKDVSPSGAPADSKPNETAADIKPIVQEGEILGPAAINGAKHNETAADSKSKEAEVGNKPHAGRDLDLEEGESLRSATNEGTPDPWFKFPAAIDDASPNGAAADRKPNKTAADCKSNETEVDTNPNGGPDLEEGEILSPAANGTTKPNGGPDLEEGEILSPAANEPAIETDGLLWANGLFTDLGLATFLEDLKTTGCFQGPLTDGQFGQVLGQLRSTHGFSHLYTQYLDTRCVSTPKRLVEMLRQLWLDESAHGSPCHYPECIACHDMEFEVDDSGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0G5</accession>
    <name>A0A5B0M0G5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033852</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0G5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="73" mass="8286" checksum="D4F7DD8E09EF0A17" modified="2019-11-13" version="1">MHRRVLLRVFGRISVRFGSGFSCSRLLINFLIINPNSYQTDRQLDKGFSSEIDQTDSPLVLICTTDNSPLIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M201</accession>
    <name>A0A5B0M201_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGT21_004356</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M201"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000702">
        <property type="entry name" value="ABC transporter (Adp1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF2">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022925707" description="ABC transporter domain-containing protein" evidence="15">
        <location>
            <begin position="20"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="819"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="851"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="893"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="932"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="959"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1049"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="93"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="407"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="676"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1070223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1072" mass="118838" checksum="07259932A5BEC7BC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M560</accession>
    <name>A0A5B0M560_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclopropane-fatty-acyl-phospholipid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023827</name>
        <name evidence="2" type="ORF">PGTUg99_020562</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFA/CMAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M560"/>
    <dbReference type="OrthoDB" id="506498at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000554">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43832">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43832:SF1">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="43792" checksum="D9CA6C69502172F8" modified="2019-11-13" version="1">MFQQLYQQLEAFGYQAIDRGLIPDWILRRLIRFSCALRIKELDSPSLEELHRRKLSFIDSLRSLPVAVHQKSANEQHYEIPTELMKSCLGEFMKYSCCYFPKMNESLDEGEILMLQDYCKKAKLEDGLEILDLGCGWGSLSLFLAQKYPNSRITALSNSRTQKIHIDQVAKRRELKNLTVLTDDVMVFDFPSDHARFDRVISIEMLEHMKNYEFLFKKVSTWLKPEGLFFAHIFCHKDQPYHFEEKDGWMAQNFFSGGTMPSLDLFAYFQRDLNLEHSWFINGTHYARTAELWLNKLDQARSVWTSENQMLQVMKIQELDEESATKEIYKNFFKFRTFFLAVAEFFGLNEGQTWGVGHYLFSKKTLASKIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5C4</accession>
    <name>A0A5B0M5C4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="3 11">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">HAT1_1</name>
        <name evidence="18" type="synonym">HAT1_2</name>
        <name evidence="17" type="ORF">PGT21_006684</name>
        <name evidence="18" type="ORF">PGTUg99_009867</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="18 20">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic component of the histone acetylase B (HAT-B) complex. Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. Involved in DNA double-strand break repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the HAT-B complex composed of at least HAT1 and HAT2. The HAT-B complex binds to histone H4 tail.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the HAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 11"/>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000211">
        <property type="protein sequence ID" value="KAA1123115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5C4"/>
    <dbReference type="OrthoDB" id="10253098at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000072">
        <property type="entry name" value="Histone acetyltransferase type B catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013523">
        <property type="entry name" value="Hist_AcTrfase_HAT1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21184">
        <property type="entry name" value="HAT1_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038084">
        <property type="entry name" value="HAT-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="16">
        <location>
            <begin position="9"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="233"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="13">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="13">
        <location>
            <begin position="241"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="12">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="264"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with histone H4 N-terminus" evidence="14">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR038084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1071419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1123115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="424" mass="50069" checksum="1E51F4FA22B75058" modified="2019-11-13" version="1">MAGEDPERWACDGTGALSLRLARGKVAADQLAPSEQHLISSFHPKFVYPLFGDEETIYGYEDLDIQLAFNSSTLKNYLAINYAAALPEPDPVEKIIYKFIPPDYTKSEATFNADLESSGTKKFIPPGKKISSYRTLPSKGKTKSEHFLTSKRWERCTDDQMEDEQDVIYELWAANWNSPGFREYHRRMQILVLFYIEGGSYIEEDDDRWEFVVLFERRKVKTADDDSSPYSYHFCGYVSLYSFYHYPSSIRLRLSQFIVLPPYQSNGHGSMLYSQIFQYLLTRPEVAELTLEDPSESFEDLRDREDLKLLFDNKVFDEKPLNELVPVPSEWYEKTRTKWKLANRQFARLLEMALRWKFTTLEESDNTKLERLYRIAVKERLYRFNYDSLVDLSKEERREKLQDTFQNVMDDYDRLLRGERRQID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6T8</accession>
    <name>A0A5B0M6T8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023699</name>
        <name evidence="2" type="ORF">PGTUg99_020058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6T8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="126" mass="14388" checksum="0CFAEAAACBE864B4" modified="2019-11-13" version="1">MFLKLLIFGLFGVLGIFGAFISSLPKLESGAKSSGSLPHLCALPSIRKDDKPLQKSLFIQFIEGFQHHLNKMSLEKRLKVIENWKINEEELMVLHQDSEDLQVLRVDFENRFTESQILGSEAKRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M748</accession>
    <name>A0A5B0M748_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026904</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M748"/>
    <dbReference type="OrthoDB" id="2510764at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022834360" evidence="2">
        <location>
            <begin position="32"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="459"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="557" mass="62236" checksum="CC4486B17C81D80F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7G5</accession>
    <name>A0A5B0M7G5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023448</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7G5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="10859" checksum="4AE38D5EDA692409" modified="2019-11-13" version="1">MAPLASACGFGSWLRCSRVAFFFSVTTTPNSDTRRPGQRRLGRNDTYAVKRCGPKASPETSIKLQLAIAGQQFKGASPRHSLIFIQFHIPFTINTSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBN7</accession>
    <name>A0A5B0MBN7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011136</name>
        <name evidence="2" type="ORF">PGTUg99_017304</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBN7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1122001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="89" mass="10642" checksum="4ED2FF6AB1296AF7" modified="2019-11-13" version="1">MFMCQENSPGLTVHKPRYMEYGWRCVKPPRTHIGRSFLIFSIDFKKLFEFRERCRGSNLIDLHSGPRVRQRKHRDEIDCIPTQQTPSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBQ4</accession>
    <name>A0A5B0MBQ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010941</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBQ4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="11700" checksum="59D00129BA940B08" modified="2019-11-13" version="1">MRVARYTGLKGCLYYRHAGISFQHEKEIHLLTGFNAKCPDFSFQNQNEKLRNWKKVSSTNKMPRLIPYPVKLTLRKGSLEITIKLSKIKSWVASLISKICT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDS0</accession>
    <name>A0A5B0MDS0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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            <dbReference type="Proteomes" id="UP000324748"/>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    <comment type="caution">
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        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="783" mass="84800" checksum="3527FC510D7406F5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MI09</accession>
    <name>A0A5B0MI09_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="12">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_027216</name>
        <name evidence="14" type="ORF">PGTUg99_014493</name>
    </gene>
    <organism evidence="14 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="14 17">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="12"/>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MI09"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF134">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="272"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1075803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1091153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="309" mass="35201" checksum="DFAAED70FC927CAD" modified="2019-11-13" version="1">MAILWELSTKLFDQLPPIPSHFTQWEPPQSPLSTYSGVFAAIVTYLSIIFGGQRLMADRKPIQLKPLFMLHNILLSLGSLWLLVLMIEQVAPIVYNHGIFYSICHVNSWTPELVTLYMINYYFKYWELLDTCFLVTKKKSLQFLHVFHHTATALLCFTQLGGRTSVSWVPICANLTVHVIMYYYYFTTSAFPGYKPWYKKALTSLQISQFVIDLFIVYFASYSYFAAEYLGWPTMGNCSGTEGAAVFGCAILTSYLFLFIGFYRKTYKQKSLNNKKPSSSSTTTTTPTTTTNPVSSSTDNDSLLLAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJP0</accession>
    <name>A0A5B0MJP0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001201</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJP0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="211" mass="23494" checksum="56C75A3A3338A2EC" modified="2019-11-13" version="1">MCLNQLTTMKHHLGGILLKSIHNAHHPGRTNPVHNGTDTTIFQSIACFTRVGWKCYLNSFLGTSGQPSQGVAPQLNPCCPSSHPFPIPLSAPSDMDLYTLHRSGHADVDIHPRLHATYIGSSFDYSQQDTSSQIDWNRGRNQEDSTRAVQSTIKINTVRGTSKLSRHQEFHSRTLSILSTYSPSSVFVQCHPLLITNSYFIHNSLKSATRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMN2</accession>
    <name>A0A5B0MMN2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033873</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMN2"/>
    <dbReference type="OrthoDB" id="2505887at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="30759" checksum="7BF9E9B6CCBD0789" modified="2019-11-13" version="1">MSSATPSYFSFNCTDVLTETAPTSLNTDQTPSYMRSLPNVSELLDPALNFWGEEESRTHILHSAPISNYQSHSTVQNESWDSSPSLETFRGEEQFVLPGEPILCKTKRNSTEYWPARLISYEGFSNMCSTNPIPEKLYWVHFCDEERVLLPRTCFLTAFDEGFRTVNVGQLTTSEGTFDEILPKLLKELPKLDSIIAGGYADQSVRDQHKNHLSGITEAHGRLNTNFGRHSEAIVTSIGDYLRDRPSLLIPWIRDFYNSLMKRKPLTFMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPC9</accession>
    <name>A0A5B0MPC9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022604</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPC9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022934740" evidence="2">
        <location>
            <begin position="24"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="279" mass="31561" checksum="5F6BCD6A76420FB5" modified="2019-11-13" version="1">MSEAHYVILQVHLLLTPDTWTLANPNGKPWQMLTNIKEFPRRPIDGPIPSLMELVMIFTTTLTRTPANNITQWDLVDRQLEHIRRQSDNYKHAYARVVIRKDQEYFGTRELHEIDPNSVTLPSDEEIAEEMARAEGGPLRLGNFERRSSSVVDYIVIKLSASITPSADTIVAYRFSNNPDELSKTPGRPHPVKPPFASPRSLDDCPMPCLRAVMKTNRNLHYRPATLCGVNDPLDPARADKDRRDNWLDPVKGEKTFTLSCIDGAPLPELVNMLALASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSE7</accession>
    <name>A0A5B0MSE7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026873</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSE7"/>
    <dbReference type="OrthoDB" id="2506354at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24415" checksum="E41AF79346644BAD" modified="2019-11-13" version="1">MASEVDRLMIKNTSINPHLSKNHIRCFCHKIALILNAGLKSTQLPTSGLLPSQVENLGFVPRLSPIIEESEEIEEAPQFTVEDVILGVDNNHQVEHEANDEDVIDGMDSADPESWEKAEEGKNPLDKILKKVDFVIQRITSSAVKRSEYKTWSKKLEIHGPSLIAGYGIRWNIKFESRTRAYQARRIIKKLIENEKDRQEQEGGKNYYNDTEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MV14</accession>
    <name>A0A5B0MV14_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004112</name>
        <name evidence="4" type="ORF">PGTUg99_020070</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1079996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MV14"/>
    <dbReference type="OrthoDB" id="2500136at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1079996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="424" mass="48163" checksum="85099E7BAA0194F0" modified="2019-11-13" version="1">MENPGSIAKLENHINNLQSLLQSISTIRYQSLPSLISNLQKPIQACQPNHDQPSTSTEQLKEIKDNWKSDSQELINRIKILRQQASNSEEAMKYLRAKTHINNSLTPEEKAKAFHETYLINQKFKQEAIQKFKSRSSLSSKRVKTTEPIIHQDIVQAELLKRKKLILLKRRASSFPTKPSVPVINLQDCIQRINHLDPLSAAHLVFVSHNQILVKEVMRAFLVFKPRTPQQIAPNLIVERVFCFGLTESPTTTFQRSSFGLIRSINRCINHWINQHAQSDRNPTSSNLWLICSLLSSYKDFFKTEISVQSRAGNTATANPRPTQLHPPHPHQPPGASRPTTKQVQINRDNTQSFLTWRCWKLDPASIDKLIAADQPSPPPAVDGQTANPDQQMNLADDPASRPAVDPLQELFRSGYWAKIPEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MV38</accession>
    <name>A0A5B0MV38_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc-ribbon 15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022627</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MV38"/>
    <dbReference type="OrthoDB" id="5545479at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139:SF1">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13438" checksum="251720F9050CB1B0" modified="2019-11-13" version="1">MCDECCICLSVGCPTKINPSGGDGQARICPRCNNGSVFQAQSQRWLEVCFLPLFPFKTKDVWSCNICSWQAELGTFQPLVASTFPPPSFQTQPGYSPPMMYAAPYPAAPPPPATYTPSSGAVPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVD9</accession>
    <name>A0A5B0MVD9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Pyruvate kinase</fullName>
            <ecNumber evidence="6 17">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PYK1_2</name>
        <name evidence="20" type="ORF">PGT21_025712</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="6 17"/>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVD9"/>
    <dbReference type="OrthoDB" id="108365at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00288">
        <property type="entry name" value="Pyruvate_Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000025">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.20:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 17" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 17 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="15 20" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="18">
        <location>
            <begin position="176"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="19">
        <location>
            <begin position="538"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1080935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="674" mass="74008" checksum="588D5267F64E35A0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N475</accession>
    <name>A0A5B0N475_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011367</name>
        <name evidence="2" type="ORF">PGTUg99_030644</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N475"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="84" mass="8949" checksum="7EFC9A0A796405B3" modified="2019-11-13" version="1">MHLRAKTLKRSRTSSAETIANGGAKPSGVLTAVVRMLFMEAIIGMIAERQTAVGVGGRELEGTKEKLHEGDNNVFVGVGCRPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4H3</accession>
    <name>A0A5B0N4H3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018729</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4H3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="11199" checksum="CC516C2902B3B8A9" modified="2019-11-13" version="1">MAHSHAPPLSSPLAHPGPRPAKALLFGATPCLPKQCILIELAQARQFPYLTSSASHQYRFTPKGIRLFALKGSNFHIRFFLEIDCPWFQEKMTKLKNAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8B8</accession>
    <name>A0A5B0N8B8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010865</name>
        <name evidence="3" type="ORF">PGTUg99_011804</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1135961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8B8"/>
    <dbReference type="OrthoDB" id="6749203at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025314">
        <property type="entry name" value="DUF4219"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13961">
        <property type="entry name" value="DUF4219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4219" evidence="1">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="208" mass="23215" checksum="8F7B86F486875568" modified="2019-11-13" version="1">MTPETFIAVSQKTLSSSSHHLGLIIVTLILKTCHIVPALRFTVSLAQSPRSQFITQTKAGSMSGTEKHDTPASNGVPVLDGSNYTNWHSRMFIFLRGKKLWKCCTVPIAEDATEEERTAYEEAGDKAISIITSRINHRCYNEVVNSLTDSDPIALWKKIASQYASHSVINRGRCGKPILNLKAQQGYISYKNHRLKAPQEFTQAPKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8P9</accession>
    <name>A0A5B0N8P9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8P9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="67" mass="6645" checksum="CDFF8A9A14D4412A" modified="2019-11-13" version="1">MPQTPGHVRASLTALLMRDLGPTSAGREPFLASSGHAEQAADGSGGGPKLPSAATFGSFGIPSSKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N988</accession>
    <name>A0A5B0N988_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022542</name>
        <name evidence="4" type="ORF">PGTUg99_002447</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N988"/>
    <dbReference type="OrthoDB" id="2497742at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="341"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="761"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="774"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1113722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="784" mass="88279" checksum="CF5E6F03D99A1901" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND16</accession>
    <name>A0A5B0ND16_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013388</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND16"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="90" mass="9980" checksum="3F9F141C4B1B0C6B" modified="2019-11-13" version="1">MTHSPPVIYTLHGGCNVASGQQSAQSDPTGKVNNRLVAHGQFEWPLQSTVQPVKSHDHENSSMTAYNVVIKQYDWPIPFGSLTWARHGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEL0</accession>
    <name>A0A5B0NEL0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_020407</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEL0"/>
    <dbReference type="OrthoDB" id="2506755at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="128"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="345"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="438"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="568"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="738"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="345"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="455"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="589"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="631"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="739"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1087042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="802" mass="89838" checksum="62B74C54CA8F7421" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFD0</accession>
    <name>A0A5B0NFD0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFD0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="163" mass="18205" checksum="254801CE7BBE5325" modified="2019-11-13" version="1">MVKNLEGVGQPTGFGYTHVPLEGFSDVTEDFSILSFLDHIHLHRTFKYINEKLVSGTHIGSYWFGFQFREVLASSWVQDNGSAVAASRPSIRYCMHTSVEAVSKIPFQARKYLCTKNFLSTPPSGRFAARPFHRAGDKSRSSRERGTLRARPAISGPPIAELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI59</accession>
    <name>A0A5B0NI59_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI59"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="7496" checksum="FA519A45ABD6A631" modified="2019-11-13" version="1">MTRLKVGSNWAARRAGAVGSLGAGPAWSVFSIPNARGQPGYNRGQPAKTRAARGLPAGSLNRKRVRVGLPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNQ3</accession>
    <name>A0A5B0NNQ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_001679</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNQ3"/>
    <dbReference type="OrthoDB" id="419616at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF15">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="21"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1090453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="270" mass="29498" checksum="650AED667A19C893" modified="2019-11-13" version="1">MPNSDSDHPQPPARTNRRNLQLFILCLIRLCEPISFTVIFPMVAFMVAEFNPTLSEKEVGFYCGAIESIFSLAQFSTIILWGKLSDRIGRKPVLLIGLLGVSISTLAFGFSSSFWTMIAARSIGGILNGGYLSRPAERFPHSWFGTSSFWQNHPWLLPCAVAAIAPLLGFVMATLWLKETLIKKNPTNGERAPLLQSATDIDAQEPLAPTAPTPAPPTKIVDLLKDRNLLIILISYSLLSFQTISLEALIVLFAYTPIKSGGIGFSSAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPE7</accession>
    <name>A0A5B0NPE7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear protein localization protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">NPL4_1</name>
        <name evidence="21" type="ORF">PGT21_011495</name>
        <name evidence="20" type="ORF">PGTUg99_012128</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">21-0</strain>
            <strain evidence="20 23">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Involved in the import of nuclear-targeted proteins into the nucleus and the export of poly(A) RNA out of the nucleus. Has a role in the endoplasmic reticulum-associated degradation (ERAD) pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NPL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPE7"/>
    <dbReference type="OrthoDB" id="10251089at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08061">
        <property type="entry name" value="MPN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000243">
        <property type="entry name" value="Nuclear protein localization protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016563">
        <property type="entry name" value="Npl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007717">
        <property type="entry name" value="NPL4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024682">
        <property type="entry name" value="Npl4_Ub-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007716">
        <property type="entry name" value="NPL4_Zn-bd_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710:SF0">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05021">
        <property type="entry name" value="NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11543">
        <property type="entry name" value="UN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05020">
        <property type="entry name" value="zf-NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010052">
        <property type="entry name" value="Polyub_prc_Npl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0811">Translocation</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MPN" evidence="19">
        <location>
            <begin position="245"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="624"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="624"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1074071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1090713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="698" mass="76773" checksum="E6A887364C0FA7CE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPF4</accession>
    <name>A0A5B0NPF4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Adenosine deaminase, tRNA-specific 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ADAT3_1</name>
        <name evidence="4" type="ORF">PGT21_027101</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family. ADAT3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPF4"/>
    <dbReference type="SMR" id="A0A5B0NPF4"/>
    <dbReference type="OrthoDB" id="3180714at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF156">
        <property type="entry name" value="INACTIVE TRNA-SPECIFIC ADENOSINE DEAMINASE-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1091147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="436" mass="49538" checksum="BDDDA76539407493" modified="2019-11-13" version="1">MSRTWSKEDEEQKEGLEKVIEIIDGYQQHLQQMEDAARQSHHSEETTAFFPFELVTQQNPFSKNLHTNTSVSGSAVEEGPEDPDSYSLEVWIIDCDHPRYISALFQFIRAHIYQDSRNENDKQSIKHLKSIKPILHDQRIQDSVDEGRRRTTNGVSVLLSKTSSISYQELTARLQTHPILGDLAAKSTPYRIRVPTKRALYEEHYRHHVVEERGRMKKKKNAAGWPMVELDESALSKMMSSTMQANQRILQRRWTVAEVGWLLTQAKLVAQLALTSKLEAHEIGVGAAVSRYSLDPPEELAHLPRDQVSNPILCASVDRRKSSSNRLRHAVVELTTAVSAQDLVAPRRPQSALHPSPYLLTNQIVFLSHEPCLLCSMALLHSRIKHLFFLAPSPRSGGCGSVYNVHEQDGLNHKFFAWKLSFPEPLSPLLNTFFDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPK4</accession>
    <name>A0A5B0NPK4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPK4"/>
    <dbReference type="OrthoDB" id="2498398at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="488" mass="55650" checksum="48CEDE40C38C5965" modified="2019-11-13" version="1">MALRHLMYSASHAQSVYDMNRDVGLTSLLAIHSRLLPLFRDLIIQLVSSLEVLDFENEPIPELSLFPKFAELQHTMEQAVSAVVSVALRPDQPDIEVDGNCKDFKIFRTSRLLEDIRELQDLNWALFEVLSELIQGPTPIDDPDTEQNPVDNQDQDIGAGWRVIDDPDPDQVWVTNPNRLGNQDWVGNQDWFGNQDWGGVQDWGGVQDWGDNQDGGANRDWADNHDRWEDPDQERNPGNDNANAEQANADAEQAPEDTDAQNTESSGKEESWTTKKKREMISRATLSTDFIDAMIRRCKQSDFALLHETWQAAVGDIDHALSFFCGKSPDGISRRDDIARLTRSATALMKVTRVFYSRLSNTSTHLLPFTLPHDVSSDELELMLWKTAPLARIIDDELFVLIRQINCVNTITIWQQSIITQIVREMQKEFTVAWTFLKRYLVPLTPKAGHPSSAPFVFDDHFQDLKDQFYLACDNTIEFADKVEDPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NU36</accession>
    <name>A0A5B0NU36_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NU36"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="134" mass="14504" checksum="1774B7482C62BA20" modified="2019-11-13" version="1">MPKKNYAALLSNALADVDLNFPSVQWLLNSILRPLEHLTKAVTKISRASSDQKSGDSTTGPASTIEVETLGLYEGELEPGNQDNLPNTSEEEDHFDDEDANMDDLDDGAMFGGSDLSDASDEEGDVTIDKGEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NX25</accession>
    <name>A0A5B0NX25_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009331</name>
        <name evidence="3" type="ORF">PGT21_009347</name>
        <name evidence="4" type="ORF">PGTUg99_024927</name>
        <name evidence="5" type="ORF">PGTUg99_024975</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NX25"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1093738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1093739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="80" mass="9327" checksum="087E352F7D9505B6" modified="2019-11-13" version="1">MHTFRLKTWFSLLPMVYLFLVTVINAMVVDPAMAKMAKENLLEIPSTEPFMMRTDPDINSQEWYCDECRKNHDPKAGCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NXK3</accession>
    <name>A0A5B0NXK3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutathione hydrolase</fullName>
            <ecNumber evidence="13">2.3.2.2</ecNumber>
            <ecNumber evidence="13">3.4.19.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Gamma-glutamyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Gamma-glutamyltranspeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_002486</name>
        <name evidence="16" type="ORF">PGTUg99_005965</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="16 18">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione conjugates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 13">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="13"/>
    <dbReference type="EC" id="3.4.19.13" evidence="13"/>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000115">
        <property type="protein sequence ID" value="KAA1129982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NXK3"/>
    <dbReference type="OrthoDB" id="1081007at2759"/>
    <dbReference type="UniPathway" id="UPA00204"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000001">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.130:FF:000005">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055262">
        <property type="entry name" value="GGT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF9">
        <property type="entry name" value="RE13973P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00462">
        <property type="entry name" value="G_GLU_TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="454"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="472"/>
            <end position="474"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="524"/>
            <end position="525"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="553"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1093905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1129982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="651" mass="71663" checksum="F63324DF3C7D251B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3P3</accession>
    <name>A0A5B0P3P3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006338</name>
        <name evidence="3" type="ORF">PGTUg99_009560</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000315">
        <property type="protein sequence ID" value="KAA1104907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3P3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="88" mass="9771" checksum="F3F2305C69D96DD2" modified="2019-11-13" version="1">MLQLGRGGSLRDGVPAPIPEHEQVYSASVRQTAFARLQRRMGGFAQSVPVPVPHESGVHQRKELSTQFDPPVKDTTYRSPPRPHMKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3Y1</accession>
    <name>A0A5B0P3Y1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029498</name>
        <name evidence="2" type="ORF">PGTUg99_031435</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3Y1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1121538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="75" mass="8496" checksum="6EB15B6F4DC27355" modified="2019-11-13" version="1">MLEHTQHTYPTERYTVRPLELIDQFGSSDNIQLIGCYTHSEGGRRDSFGCDHYCDHEGLLMISPQLLGALPVALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7J0</accession>
    <name>A0A5B0P7J0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7J0"/>
    <dbReference type="OrthoDB" id="96345at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="68" mass="7554" checksum="CB46951EF3ABA385" modified="2019-11-13" version="1">MASSIAITMMTLLQRSHDQASKWESTTGSAITAVGVDGSTVIVRRHLLRICRYWDELGPIMRPAVDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7R7</accession>
    <name>A0A5B0P7R7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LNS2/PITP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_012749</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7R7"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000063">
        <property type="entry name" value="Nuclear elongation and deformation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="4">
        <location>
            <begin position="765"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="110"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="155"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="303"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="553"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="615"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="676"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="985"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1021"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="556"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="572"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="584"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="621"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="683"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="985"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1030"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1041"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1054"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1067"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1118"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1149"/>
            <end position="1159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1097597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1174" mass="128803" checksum="2FD5899689491189" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PE04</accession>
    <name>A0A5B0PE04_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022376</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PE04"/>
    <dbReference type="OrthoDB" id="5946233at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="2">
        <location>
            <begin position="189"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="499" mass="57411" checksum="97B1FAB50827AC73" modified="2019-11-13" version="1">MDILTSDYPNTSGTDDDPNPDESDQSTIKDHTNSNTLTSDDLVKLIVQSLLTSGHKGPKILQILREQHGISMSASTLTRKRQTWALRQVDLPQKPPKLLDPTRYMNQLQVKILRNDVADGKVTMVEVYEAVAHAREYLLQDQAGYRRMRTILMRNYKIQIPRQMVYDAFKHIDPEGMAARLRQTCKRRIFRTHEPNHVWACDGHDKLKKFGITVYGFIDAWSQKILGLHIHVTNNDPHHIGVYFLQLVQKVGGVPRKVTADHGTETPDMSWYQMKLSHEFCGITLEEAKNRMHHTKSTRNQKIEALWSQMMKQHNQSIIDNIWSHIEAGVYDREDPIQKLLFLFLWIPVFQASVDIWVDLTNNSRKQKDHKISLPTGCSPKFSYGTPEAFGTTDQLVVVDVDQVNNYLTNDYPNIDSMFTHTPPWFHNVASRLLSQLGIRFDHITVGNVWNVFGHIQAHFTLYPPSPVDLLEIPPSPDDEESDYDTEDSHDEQQNEGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEM7</accession>
    <name>A0A5B0PEM7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028789</name>
        <name evidence="4" type="ORF">PGTUg99_016492</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000144">
        <property type="protein sequence ID" value="KAA1128151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEM7"/>
    <dbReference type="OrthoDB" id="2515503at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474245" evidence="2">
        <location>
            <begin position="19"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1128151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="618" mass="72775" checksum="8C16E807398BD7A4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PF03</accession>
    <name>A0A5B0PF03_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012922</name>
        <name evidence="3" type="ORF">PGT21_022851</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PF03"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="137" mass="14843" checksum="6E0F4854CDBCD112" modified="2019-11-13" version="1">MIIFAGLALYMLVACVLILVKAVKDGANATLYAQIVISLITTLGKMDPCCYYSIDVDLSGILCTIGSWITSSILALDLWRLLTLMLHYLLLAPAYINVLNVYALANLEGIPVAKADCTEQSELCAKHEIQGYPSSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGP2</accession>
    <name>A0A5B0PGP2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small COPII coat GTPase SAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Small COPII coat GTPase sar1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">SAR1_1</name>
        <name evidence="23" type="ORF">PGT21_019518</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPII-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGP2"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00879">
        <property type="entry name" value="Sar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000161">
        <property type="entry name" value="Small COPII coat GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006687">
        <property type="entry name" value="Small_GTPase_SAR1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45684">
        <property type="entry name" value="RE74312P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10 21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 21" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="13 21" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 19" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 21" id="KW-0653">Protein transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 21" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAA1099754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="289" mass="32694" checksum="8593F77920028EBF" modified="2019-11-13" version="1">MFILNWFWDVLANLGLVNKNAKILFLGLDNAGKTTLLHMLKNDRLATLQPTLHPTSEELAIGNVKFTTYDLGGHQQARRLWKEYFPEVNGIVFLVDAQDPERFSESKIELDALLSIEELSKVPFLILGNKIDAPGAVSEEDLRHCLGLYQTTGKGKVPLIDIRPIEVFMCSIVMRQGYGDGSSPSRLPLTSHFIIDCSCSSSLLDGLGLCYRLPLVGTIHLRSSLNQKSPNQDFPFPFLFFFTTTILSLSFSFLHPYIVLFCLYHSFFVFFLTHPYLTKFLLLLSLHLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKU6</accession>
    <name>A0A5B0PKU6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Surfeit locus protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SURF4_2</name>
        <name evidence="8" type="ORF">PGT21_009047</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKU6"/>
    <dbReference type="OrthoDB" id="7859621at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002995">
        <property type="entry name" value="Surf4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02077">
        <property type="entry name" value="SURF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1101148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="315" mass="34757" checksum="A310EDA316D57196" modified="2019-11-13" version="1">MSTYTPVPLKDLDHNNPYHSNAGGPLPSTGPSRPPFDDQGESRLTRATQLLRKYSAPIEALIENLSRPVKPHLNKLARFLLVSTFLEDALRISNQFYEQKEYLIDDQEYHDWFVVGFLAANVILMVVCSILIIFKKWALFSVLGLVGVIVAQGWVYDLLTDGTFVCLNLSIMGALMLAISDSIVSKSVGGSVRGMAGLPDISEDVARKRRTYLQLSGRILLVVFFVGHALAAYLELAFDSFSFLHLAAAALAVLACLLVAVGFKAAWSATFLVILTSVVNVLSNDWWSKPKDHPERDFRRGGCKQKGVQLDGAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNX2</accession>
    <name>A0A5B0PNX2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNX2"/>
    <dbReference type="OrthoDB" id="2508363at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="364" mass="42260" checksum="E0CAC4D4FBDF7C0C" modified="2019-11-13" version="1">MTKQTQPLRSLERELNSHTPLTTDPMDIKARTVNLKENTKLIRYYLQFLKLRTEEFVKNYKPSNWELLSREEQLQIVIARIHREQDDTLAAKILVNETLREYREYKKEQRLKMSASTTLANTNTTTLAVVPINNQAATNNIGNLEDTSVDELDELLNYPCDPTLPDRSQHQLDRGAQLPLKERVRQLSMLQIKKKTTETTSSPITNTSQNKTREEAMDLDDPSTSLEDIPKRPNSPEIVALSKKEKICLLIKEHVAIWTRFEKEKISGATNKLRQILHQAQDSQKVLQRMITKEELEGYVNGWNPWTAKRESFPPPPKKEGRKKSSTSKRDQQYDDPRVWADVFEIGQAWRAAYRQRSRKGLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRN9</accession>
    <name>A0A5B0PRN9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRN9"/>
    <dbReference type="OrthoDB" id="2509948at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="525" mass="56420" checksum="C00FE2F65517868D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PS59</accession>
    <name>A0A5B0PS59_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbohydrate kinase FGGY N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_010850</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PS59"/>
    <dbReference type="OrthoDB" id="203824at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019150">
        <property type="term" value="F:D-ribulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019321">
        <property type="term" value="P:pentose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435:SF4">
        <property type="entry name" value="FGGY CARBOHYDRATE KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435">
        <property type="entry name" value="RIBULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="3">
        <location>
            <begin position="11"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="345" mass="37284" checksum="99AD85205270AC62" modified="2019-11-13" version="1">MTTDQKSNDFFIGVDVGTGSARAALVACDGEIVAESTYPTTTYRDDHNHDIFEQSTTEIWNSIAKACKDCLRDAKVGPEQVKGIGFDATCSLAVTTFEGEPMSITQDQWGPGESKRNIILWADHRARDEANLINSCGSPFLQYVGGTMSLEMEIPKVLWLKRHMPHQLFNQSMFFDLPDFLTYRATGNLARSNCSLVCKCSYVPPEVEGHKGWNDDFFRKIGLPKFVKTDFKQVGGSPGKQGLILTAGQPVGMGLTAKAAGDLGLLPSTPVGSGVIDGYAGWMGVVAAKMEGEKDSDLGMSQHRLCVSAGTSTCHIVQSPNPVFVPGVEGPYLHAVFPGCESVYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSD4</accession>
    <name>A0A5B0PSD4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSD4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="166" mass="19138" checksum="AFEBDC452E7AD1E4" modified="2019-11-13" version="1">MSQVYSVKPNYSLFSTIWAVRTTIRIQRILNHILNSNHHRAKPNCLSTLEYKSCPDLDQNLLQSSSVFHQPDFGIKPRTQLNLIFSHQQLRLTIESVLLQSQTHSSTHRLICQFNLVENFIKKPLSIFQAHRGSLRLTVELLLPMTDVEPKTSTNKNEDQKTATDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT43</accession>
    <name>A0A5B0PT43_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT43"/>
    <dbReference type="OrthoDB" id="2507699at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="672"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="762"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="799"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="835"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="872"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="912" mass="95229" checksum="1896AB47DF493D51" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT63</accession>
    <name>A0A5B0PT63_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002973</name>
        <name evidence="1" type="ORF">PGTUg99_015526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT63"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="219" mass="24042" checksum="FD8E1344A7353E3B" modified="2019-11-13" version="1">MSDLHPSLVQSRNAIIKITPEHVGSSSSTYYVLAHGLGGPLGLHETIQSLSSNKLRNRYASMEEATIHLAGLLSCSNSMYMRITFGPSMFAAGRRVRWIPGEVQNPPYRLNAQTDVRVHANLLRARRPWCIPAGGHIYVFLSASGLLAGANIAAASPRLTHTSPLIELQHQVNKKKPLVERTTACAVHMDMADLKADAHDFSKSGQLLRMIVNRARLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PWL9</accession>
    <name>A0A5B0PWL9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001498</name>
        <name evidence="1" type="ORF">PGTUg99_007763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PWL9"/>
    <dbReference type="OrthoDB" id="2505867at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.140">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1088292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="255" mass="26419" checksum="F86BD63C7EA29A02" modified="2019-11-13" version="1">MQVPAVMNSTASPMNATVMAGNATDAGGSIIPGLPSIFPKPSSGGSGSGFPKFPLPNVTPSVNQSCVAGIKSLFAAAQDSQGQPMPRERVSCFKPYTKVPDGENLVVASVDAATNVTASISQLVQDVSGKRITAAIPIIISDLRTLTAVVATGVAHIALATAKGVFTSSSLVYVLQGFVTAGQSVTDALIGQKGFFAQFQALTPIRNTLLGLKSVVDKFVLLLIHMIPTNKLTLASDLHQQMSNSMDKAISAFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXJ6</accession>
    <name>A0A5B0PXJ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_014401</name>
        <name evidence="5" type="ORF">PGTUg99_019810</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000345">
        <property type="protein sequence ID" value="KAA1098884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXJ6"/>
    <dbReference type="OrthoDB" id="439993at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="4">
        <location>
            <begin position="56"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="302"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="509"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="511"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="529"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="542"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1098884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1105651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="626" mass="67692" checksum="8A8B049802FB5637" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXZ4</accession>
    <name>A0A5B0PXZ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXZ4"/>
    <dbReference type="OrthoDB" id="10500059at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="239" mass="26653" checksum="978FB44FA91E1882" modified="2019-11-13" version="1">MGCLDFEIVFRLGRKSSKPDALSRQPDLAPSKEDKLTFGQLLRTENITPNTFAEVAEFDCWFEDKSVDLDDAKHWFQVDILGVEELNTPSEDIPTDSKLISQIRSLTPSDFRLTKLISACENPVSSQMKEATKHYLVKDGVLYKGGVESKARPRRFIVLLRSATHPTPVCCLGLIHDSLFPPASHNVLRCHPLRLRMSPLQRLSVQVLLRPLMDRSPTTASASGPLQTPLPPLGAPRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0F4</accession>
    <name>A0A5B0Q0F4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0F4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="57" mass="6632" checksum="A144EEA040A105F9" modified="2019-11-13" version="1">MFIPRSISGCRQRTHDSPQDQKATQVCRFSFDSIQDEIASQPKVDRTRPYQADRCDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1Y3</accession>
    <name>A0A5B0Q1Y3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003445</name>
        <name evidence="4" type="ORF">PGTUg99_006570</name>
        <name evidence="3" type="ORF">PGTUg99_019204</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000109">
        <property type="protein sequence ID" value="KAA1130443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000010">
        <property type="protein sequence ID" value="KAA1137224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1Y3"/>
    <dbReference type="OrthoDB" id="3366659at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41390">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41390:SF1">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 213 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="226" mass="24569" checksum="7E39F0D27C0974F5" modified="2019-11-13" version="1">MSDIRPSSFSQLTQPEFIRQETAKVVIGGVSTGIAGMTIGSLLAVQKNLPATVPGLTMCIKTSSFGTAFFALRQFVVNPILQINRIPTTSSSSSQHFYGLLPTGISAAMVGGSVNGYLRGRQVIGRSSLTTGLVCCTIQVVYNELGILKRAIFDSSGETSRTGPERPVSPAWWEAWIPLSKISDQEWKQRLSLQLAENELQLSTLNRQIDQLQKAVSNHPKHSPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q204</accession>
    <name>A0A5B0Q204_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004622</name>
        <name evidence="2" type="ORF">PGTUg99_013381</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000010">
        <property type="protein sequence ID" value="KAA1137259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q204"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1137259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="65" mass="7143" checksum="708E1158EB2C2C47" modified="2019-11-13" version="1">MTMVDTPSTIAPKDTIKGKQITTTSSILMYRDPIDLNTQQQLSDICALRRRDANYPTSTANLGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2P8</accession>
    <name>A0A5B0Q2P8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_012458</name>
        <name evidence="7" type="ORF">PGTUg99_035011</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2P8"/>
    <dbReference type="OrthoDB" id="2159336at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07599">
        <property type="entry name" value="BAR_Rvs167p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000048">
        <property type="entry name" value="BAR adaptor protein RVS167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000100">
        <property type="entry name" value="SH3 domain-containing YSC84-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF1">
        <property type="entry name" value="REDUCED VIABILITY UPON STARVATION PROTEIN 167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="5">
        <location>
            <begin position="12"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="420"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="280"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="291"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="335"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1107397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1124796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="52875" checksum="42DE2AC42137751C" modified="2019-11-13" version="1">MLKAVKRTPHVITTRVGMAKTSSDPEVDELKRQFVTVEEESAKLQKDAKAYREAVLKMLTSSHNFGQAYVTLLSPMSNEADLPERYPNAAQTIEQMAAYQELVTDLRDTVQPELDLIDTRIIAPIREFNEVIKHAKKAITKRDHKLIDFDRHNNSFTKLRDKKEKSLKDEQNIFKAEQEYEAALQDYEHYNNLLKTEIPRFLQLTTSFISPLFHSFFYMQLNILYTTYGKLQQFSEGRFDLNQTDIESIYLERLGDTHSRIEELTITKRPASTAKIIAERRAANPPPPRPVSSSLHSPRPPASLSTRTSTSSFSGKPPGLGLPPKPHSFATAGNPAKPVLAPKPSLAPKPSFSSKPAPVDPPVAAAAPPPYSPSSASQASGIGSVAAAAAAIQARKTSSDTVNPPPLKPKLKPPPPPTSQSTTYVTALYDFVAQTEGDLSFNAGDRILLVKRSDSEQDWWTGKLNGVEGIFPGNYVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5B9</accession>
    <name>A0A5B0Q5B9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5B9"/>
    <dbReference type="OrthoDB" id="10301205at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="160" mass="17187" checksum="794337DD8A44956D" modified="2019-11-13" version="1">MPSLIDETPSTFAMRTGNLPAGTSVVTSTLVDSLRKRIDTLAAQNGLSDTEITIDSTSDRAGHDATIVETQAGLPQHDSPPLAMLKTLLKDPACHQNGAVQLDNQTVSRLLQLMDTEAEEIHQLQIAAEQLARDLQTRVEAMEATPPSPANQPQPKTSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5E6</accession>
    <name>A0A5B0Q5E6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5E6"/>
    <dbReference type="OrthoDB" id="2499762at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="367" mass="40377" checksum="36AF215EF0EA0130" modified="2019-11-13" version="1">MSNSEDTRSTTRTEAGLRAMTSADVVTSILESLIPPECRSSQAWLASLATVHPSWTQPVSLALRRHPILSSISQLLGFQDSLVHHLMISLDSSPPGQDRPKPKTSPSACALRSLIINLAPTQPTTDSSTHIAHIASQERLTRQLQLSKEMYLDPGFFGSLTNLRSMDLSMLKLSLTSFRFLLASLASSLTELGIHDLCIVGAEQDTVAIFKAIFDLPRLNALRLTGRLATGRSILRLLIDQDNQLSLAGRSLQTLALSDQGLTSSGGDPMLMPHDLLHCWKTSLIPYPRDINLENGLSVSTESAAPTRYPDDRLQVLNYRVSTNAPEWHVYDQPDLVKEAAKALGLRLTVSHHSDPIQRIKWMSHSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5G8</accession>
    <name>A0A5B0Q5G8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_003250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5G8"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18009">
        <property type="entry name" value="DEXHc_HELLS_SMARCA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000063">
        <property type="entry name" value="Chromosome 12, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001315">
        <property type="entry name" value="SNF2 family helicase/ATPase PasG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044753">
        <property type="entry name" value="HELLS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="228"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="712"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="86"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="304"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="537"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="304"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1108184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="994" mass="111466" checksum="18046B332574A98C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q8P2</accession>
    <name>A0A5B0Q8P2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SET domain-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SET5_1</name>
        <name evidence="4" type="synonym">SET5_2</name>
        <name evidence="2" type="synonym">SET5_3</name>
        <name evidence="2" type="ORF">PGT21_028140</name>
        <name evidence="3" type="ORF">PGTUg99_016925</name>
        <name evidence="4" type="ORF">PGTUg99_019172</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1132992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q8P2"/>
    <dbReference type="OrthoDB" id="438641at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1132992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="85" mass="9495" checksum="70EAC92037CFD2E1" modified="2019-11-13" version="1">MTSASKASSNSSAKPPSIARLIMPLHSRFTHSCDPNAVVLKHPGYEDIHKSAPSTIYVMARKESRTNKRRQSHSRKRPLPGTRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAL3</accession>
    <name>A0A5B0QAL3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAL3"/>
    <dbReference type="OrthoDB" id="2511178at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="668"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="688" mass="77786" checksum="8200116F16F6960F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAS4</accession>
    <name>A0A5B0QAS4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Chitin synthase, class 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">CHS5_1</name>
        <name evidence="1" type="ORF">PGT21_020327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAS4"/>
    <dbReference type="OrthoDB" id="10490197at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="11762" checksum="E2F2A2B192DBFA07" modified="2019-11-13" version="1">MSEGIPSDKLVDNGKDNPFSAASPTISNLLPLGNWPSSSQSNSLNHGNFGGLLSPASNQFGPLTSLTYRSGLTCISFKDNKLVYKPVRWVAGRNAATDTVSAHICHVSLCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCC6</accession>
    <name>A0A5B0QCC6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCC6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022827778" evidence="1">
        <location>
            <begin position="20"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="185" mass="20063" checksum="5CF9F026419C6537" modified="2019-11-13" version="1">MKFLSSCSVGLGLFNSALGGTGSSTSKVTIDKVIAYRQPSDPNHKGFVVNDFGLMDTGSLCDSCENFFVAEDCIAANGKKINQVCDQAYRKGHGDDADCRSGDHTFKCSSKIVPGKHVAEKASLVNFFFSAQTWVILHTRRPWLRLLATLTSRGIPLSGPPNGVAQNKTRNAAHFDRLLMTPIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDF0</accession>
    <name>A0A5B0QDF0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_036915</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QDF0"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036121">
        <property type="term" value="F:double-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061749">
        <property type="term" value="F:forked DNA-dependent helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032042">
        <property type="term" value="P:mitochondrial DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18032">
        <property type="entry name" value="DEXHc_RE_I_III_res"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18799">
        <property type="entry name" value="SF2_C_EcoAI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050742">
        <property type="entry name" value="Helicase_Restrict-Modif_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE IRC3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME ECOKI R PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="91"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="323"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1111149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="721" mass="80627" checksum="9EE5CB8854438B36" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFF8</accession>
    <name>A0A5B0QFF8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor RSE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pre-mRNA-splicing factor rse1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RSE1_1</name>
        <name evidence="14" type="ORF">PGT21_017181</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in pre-mRNA splicing and cell cycle control.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the RSE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFF8"/>
    <dbReference type="OrthoDB" id="436637at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001143">
        <property type="entry name" value="Pre-mRNA-splicing factor rse-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000628">
        <property type="entry name" value="Pre-mRNA-splicing factor RSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="12">
        <location>
            <begin position="13"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="13">
        <location>
            <begin position="459"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="11">
        <location>
            <begin position="855"/>
            <end position="1179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1111956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1213" mass="132395" checksum="8C8A8F35BA4E441A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFY9</accession>
    <name>A0A5B0QFY9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1112019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFY9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="251" mass="28373" checksum="CFBFC510FD23DF80" modified="2019-11-13" version="1">MRFSLSHNVSTWKPMTPKWTVRCLLFAAVSHSAEKPYSSTAKVKPSLNHRSLNIIIPPRLPIILDDQLRSSLCLSTASVIGRPIHFKLLRLGHFFPPPRGESPPPSTREVADRFPPPRESTITDLRSFSTPARIDDNGSPIVFHHLEVKSTITDRRSFSTTLRIDDNGSPIVFHHLEAIPLTLHLSRIDDNGSLIVFHHLEAILLTLHLSRIDDNGSLIDLIDFNTSENRRHLSPIVLKGSRNRQHLIDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIT0</accession>
    <name>A0A5B0QIT0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIT0"/>
    <dbReference type="OrthoDB" id="2507469at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022964021" evidence="1">
        <location>
            <begin position="22"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="135" mass="13967" checksum="B5C0CB2E44EDA6DE" modified="2019-11-13" version="1">MFSASFLTLSILALIGQLVLATPQTSGGQPQMCNAYTDANTSRATCNGTPNLICTAGCRGSSAIATGCTKADGSDVNNPNRLNSPHTCNVSFGQDASGSILCVSKAKAYSCTQVASPPQMYCYGCMTTVHYHVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ22</accession>
    <name>A0A5B0QJ22_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carboxypeptidase D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_019132</name>
        <name evidence="8" type="ORF">PGTUg99_030221</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000037">
        <property type="protein sequence ID" value="KAA1135862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ22"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.410:FF:000009">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF64">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138009" description="Carboxypeptidase D" evidence="6">
        <location>
            <begin position="20"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1113034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1135862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="52891" checksum="2D83CBD4636E351C" modified="2019-11-13" version="1">MRLYLLAFLVTVLYPSAFGVSADCAPRSPTKKHHGKLNGQGAYRLIADSNICETTPGVHTYSGYINISQNHSLFFWFFEARNNPQSAPFTLWLNGGPGCSSLIGAFQENGPCKVNAQGNATTLNPYSWNKYAHVLYLDQPIPAGFSYGVHDVYSTDQAAHRVWQFFQYLFSIPHFSTYESRELSFWTESYGGQYGPKFTEYFEHQNDLIKSKKIEGHIVHPASLGITTGWFDPYYHYPSYYDYAQAKYNHVEDLVEPDVLAEMKKALDSPNGCVAQIKQCASTGNNTICRQADDFCTRFVSNPAVGGRNPYYFENPKSDPFPSESYVKYLSLPQVTSAIGAESKFTECPDGPNDDFTTTGDAARSSTPALAKLLNKGLRTVIWAGDRDFICNTLGGYRSISSMNWQHGEEFRKAPWSKLHLKGQIVGIYKQAGPLTYITVYKAGHEIPAYKPELALEVFRQTIFHENIHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ97</accession>
    <name>A0A5B0QJ97_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_021631</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ97"/>
    <dbReference type="OrthoDB" id="2505875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="6">
        <location>
            <begin position="34"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1113114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="13872" checksum="62F8521A751FCB4B" modified="2019-11-13" version="1">MDLFPEAIEASVPNQLAIYLWGKYKSLPGQAGQCLKWWKEHHKEAPVLASLAKDYLACSATSASVERCFSAAADVCGPDQGRLSIRNIERSVSSNQWLKQGLKSNDEFQTAQDVVSHDLMEKEVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QK23</accession>
    <name>A0A5B0QK23_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QK23"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="112" mass="12527" checksum="6064C46F58EB9A88" modified="2019-11-13" version="1">MPQSWSVEHDNLDFETRSHLNPTQVLPDGRLALYNALVPAVKPAPCGKDGFWGLGLSVEVALLSQICIETPIDAIRTQVPSHMPVCRRAKRDLQSQWPRRISPSELTREKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNC7</accession>
    <name>A0A5B0QNC7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019697</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNC7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="8930" checksum="544DB19E19A92694" modified="2019-11-13" version="1">MVYFTLVTQPNRTSNFVNQLDSGSILKIRQSSRFRINTQASSIFSFQAQLSSPLERRWILSSSSFFVVRGHSWNSQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ27</accession>
    <name>A0A5B0QQ27_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029544</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ27"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="55" mass="6170" checksum="1CB0271560436E05" modified="2019-11-13" version="1">MVYGSRQYFGLPMLALGFHADETGDCYGNRTRVVRGDLFVFGIPTTWHLCARASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QRR7</accession>
    <name>A0A5B0QRR7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_037143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QRR7"/>
    <dbReference type="OrthoDB" id="2218151at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="38933" checksum="C4D6D2E677DDBE36" modified="2019-11-13" version="1">MSGFLEKLRGADFTGLNRAFGRGKHSPKIPPIGGVAYESRGISGGPLSSQDNSPQARSGRGFGRIPAENGNPTNSLLDDEDTGPTQIAIRSLTIFFLFINLFIYIAIAVFQKKWGVGPSGLTGLAIFILVQALLHALVFLLVPQIYERTDMLRGFARAIRLTRIQFIVYGTQTGLALVLSLIATISAYTGGCKDSKSDPNASNTAYISHIGGFCRNKRAAAAFWWFSFLSFGLSLGIVFKSWYSIRKLGPRHPAFEPPQGVGVRTIDDEDDDFRADEPYDRSPPQFPQEMTAVRPSNVGGYYGERPIFDSDRVPAGYAAPSGSVHEVDHTRSPPQMAAYKDPFDEIRAQIYQNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSN3</accession>
    <name>A0A5B0QSN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006208</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSN3"/>
    <dbReference type="OrthoDB" id="2422411at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="65" mass="7182" checksum="B576AF721742C26B" modified="2019-11-13" version="1">MVYHQASHNTYLKKFIKDLTGDLLLAFGSLSVAVDNQLNSVHESIGKDTTKNSKANFSVLKTLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZD5</accession>
    <name>A0A5B0QZD5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004682</name>
        <name evidence="3" type="ORF">PGTUg99_002382</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000254">
        <property type="protein sequence ID" value="KAA1118343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZD5"/>
    <dbReference type="OrthoDB" id="10442956at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="102" mass="11123" checksum="D39179FD68385771" modified="2019-11-13" version="1">MVNCVHIDTLSLDLKPIDADGRPSSDVVSIRLHVHPTRLIGKRGRLDETRNTANTSSGMRLKSDGKNGTDLCRAVPLQEPIPVDRPNQCALMLMPPPKSGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R186</accession>
    <name>A0A5B0R186_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">NUO78_2</name>
        <name evidence="16" type="synonym">NUO78_1</name>
        <name evidence="17" type="ORF">PGT21_018319</name>
        <name evidence="16" type="ORF">PGTUg99_002665</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="16 19">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000512">
        <property type="protein sequence ID" value="KAA1065130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R186"/>
    <dbReference type="OrthoDB" id="10249365at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02773">
        <property type="entry name" value="MopB_Res-Cmplx1_Nad11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.740:FF:000016">
        <property type="entry name" value="NADH dehydrogenase (Quinone), G subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.210:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054351">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015405">
        <property type="entry name" value="NDUFS1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF13">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22151">
        <property type="entry name" value="Fer4_NDSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22117">
        <property type="entry name" value="Fer4_Nqo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09326">
        <property type="entry name" value="NADH_dhqG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="17" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="13">
        <location>
            <begin position="38"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="15">
        <location>
            <begin position="116"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="14">
        <location>
            <begin position="255"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="728"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1065130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1119220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="748" mass="81820" checksum="B94C4BD5742FA126" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R188</accession>
    <name>A0A5B0R188_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R188"/>
    <dbReference type="OrthoDB" id="2517348at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="439" mass="48981" checksum="2FB9CBD3028F1FDC" modified="2019-11-13" version="1">MSARFSNYFDEAAVRVQNRGPRSSNSPRRVGDLETLVNLNAHYSELECREVTRMFEGLDLRVRNLEQTAELRIRHQEHIAEVRQPNAPNPHEPGAAVGEGNRPEEAPPGRAAGADEMVLDRPEPMIHYGRAGPDANAPRDEAMLAMTQVITRVTAAVTTATVSALLDYDELFEGISDEDRTDLFAAAGVPRPHDGPDAAEVARRNGIIERNLVEFVLNAMLPRYWEDDDAFKLEYLRQLTADVKARAEADRDMWGRLFEFRRLSIEGRGRPRVRGMDADLEEFGHQIREPAEEEEAPLDPSEARRAEMEKLVKSIRNSERTIQRAGRAIQLCQHSISGDATRLATLRGEEDRHQAAQMDAGMETPQAEGPGADDPAQVVTEEENQVPRPLHPERRGDVLPRTAYRTEGEGTVTQKRGNDCGPGEGGDGGSSKRTRFERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3U4</accession>
    <name>A0A5B0R3U4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3U4"/>
    <dbReference type="OrthoDB" id="2497557at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023051779" evidence="1">
        <location>
            <begin position="19"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="182" mass="20416" checksum="68E0920F443A2EC7" modified="2019-11-13" version="1">MLFKYLLAPVALAASVVCYPPGYGGQSPSVRPDYNGLDFSSCTDKIRQVREPLASSCQRQSVQDATSKLDGLYEPIHALSNRVHVSVRSDTKVAYKYTRSLASLFSNFQGIVDTIGRYPRVFSRSQETIFEFNSQFESILTDFSSAGVNIHEVFSQSTAVRFESWQQVGFTFPEKFGFYGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4X7</accession>
    <name>A0A5B0R4X7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Iso-1-cytochrome c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CYC1_1</name>
        <name evidence="13" type="ORF">PGT21_008520</name>
        <name evidence="14" type="ORF">PGTUg99_019465</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="11">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000243">
        <property type="protein sequence ID" value="KAA1120557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4X7"/>
    <dbReference type="OrthoDB" id="449280at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000001">
        <property type="entry name" value="Cytochrome c iso-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="CYTOCHROME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00604">
        <property type="entry name" value="CYTCHRMECIAB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 9 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 9 11" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="12">
        <location>
            <begin position="10"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1117474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1120557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="112" mass="12116" checksum="BCC20AB6BA1CE5AB" modified="2019-11-13" version="1">MGKKDSGGEGDPAIGGKLFGQRCATCHTVEKGAPHKVGPNLHGLFGRKTGQAAGYNYTEANVKKGITWSEETLMEYLIDPKKYIPGTKMAFAGLKKEKERANIVAYLKDATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R5J6</accession>
    <name>A0A5B0R5J6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_024944</name>
        <name evidence="6" type="ORF">PGT21_027135</name>
        <name evidence="7" type="ORF">PGTUg99_023555</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1120971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R5J6"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001663">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:002045">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="37"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="249"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="110"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="330"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="330"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1105959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1114913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1120971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="355" mass="37823" checksum="8F03227F49783304" modified="2019-11-13" version="1">MSTTNKDAGTNPTANGTDGVATITDALEASTIQELGKKVFIGNLSFATKDDQLREMFGKHGEISDVQIIHRGTRSLGYGFVTFTTCEEAEKAVAATDKNEIDGRAINVEIAKPAPGTPGGAVPRAAARAAKASKARQTNGDAKGTDEDQDNSADAPSARRTKSGTGRTRNRRQGRARRPAGEAKPETNGDDHGAVTDASANNEGDTSSKPRSKRTRTRRPKGESSSGEPRGERRPRRVKGTPEGPPSQTLLFVANLPFSVTDEKLKELFSGYKVASAHVVCRRYGTTVGRSKGFGFVDFEDQENQLKALEEVQAKEIDGRALSLKIAVSENKKDDESAEKIDDPSAPVVAEPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RE68</accession>
    <name>A0A5B0RE68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021380</name>
        <name evidence="2" type="ORF">PGTUg99_028091</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RE68"/>
    <dbReference type="OrthoDB" id="2496900at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1124111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="256" mass="28492" checksum="383E9A089AC69D54" modified="2019-11-13" version="1">MEAMDSALVNWLAINENINRLGLPRTPRSSISAFLKPQYPMMHFLPAFVSALSCLSFATQVSSSPHKADPAPLSKTFIMKENMDYTKGALPIYGPDGAIVYSFMRNIHDPVTGYTTVVLMNPTSEPLFVLRSSNNLCAHKTAYTGSAVSGSRKQQFNIDPNGLLKDHWSFSYVDSTGTQQNYKFARHYASKEGEIYHENGDLVAELRNQKRTDSWITTPSVHEVDTYTLSYAQDSPAVELVLLMGLVMSRVHDCGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJN0</accession>
    <name>A0A5B0RJN0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033591</name>
        <name evidence="3" type="ORF">PGTUg99_007744</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000176">
        <property type="protein sequence ID" value="KAA1125599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJN0"/>
    <dbReference type="OrthoDB" id="10281008at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138136" evidence="1">
        <location>
            <begin position="28"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="233" mass="26046" checksum="55A2356192202761" modified="2019-11-13" version="1">MKKFSCSPLWLTALIIAPILHLPSTACSTIPKASPVLGQPILSKRAVIVPPRFVIIPIFGPVSFTEIFNWGCPTPTDNNRVGHGGYESRVKQVATQAIDQAVVEQYAKAHPEMAKEVSLDLREILRLEEQSGQLVSQIDHARVIKARADLARFFEAHPELQAQAEDTFQEQVNALFRLRDSADWEAADLDQVASRDWAHLTPQDDSKYKPNSALSKFHSHIQGHNLPRYTLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RK15</accession>
    <name>A0A5B0RK15_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_021872</name>
        <name evidence="8" type="ORF">PGTUg99_015233</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000175">
        <property type="protein sequence ID" value="KAA1125759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RK15"/>
    <dbReference type="OrthoDB" id="2502820at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007300">
        <property type="entry name" value="CidB/LrgB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249:SF0">
        <property type="entry name" value="PLASTIDAL GLYCOLATE_GLYCERATE TRANSLOCATOR 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249">
        <property type="entry name" value="PUTATIVE SEROTONIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04172">
        <property type="entry name" value="LrgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="521"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="588"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="159"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1070713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1125759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="616" mass="67919" checksum="7FC1B44B568F413F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RLQ0</accession>
    <name>A0A5B0RLQ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035409</name>
        <name evidence="3" type="ORF">PGTUg99_027249</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RLQ0"/>
    <dbReference type="OrthoDB" id="2505648at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="265" mass="29315" checksum="E5BB3956F0DED636" modified="2019-11-13" version="1">MTFILIPYSRTFSSRQTAYRHIKKYQQHFVGLTPSISGLYTQSQFIDRLTKDLFPSTQPNLKLLTTDLGILLKHLECDRNILISEKNVMTTICSLFFLSRLILMLSIVKGHHICCTYLDRICPHEPSRANGGNNGCAPVRSKPPTSCSNTGGAHRRKQSNASLELNPRLPSTVLISKHKPVRFARWNKSLLSVNGSPAVSYAPKKIPGLPNSTSAATIISSKSSELFPASSTSSSSLALDNHHLICCTYLELIKAGYQSNRPSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RT87</accession>
    <name>A0A5B0RT87_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CBM1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027656</name>
        <name evidence="3" type="ORF">PGTUg99_004627</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000144">
        <property type="protein sequence ID" value="KAA1128083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RT87"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138229" description="CBM1 domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9195" checksum="5AD6C530FD59A7E0" modified="2019-11-13" version="1">MQLLTPTIFIFITVACKLTTCSFPCKTSYSQGACQGQGSGTEGPNQTLKPAITANSQPGIYYCPIGEGMCCLTGPLVLSRCKRPYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RWQ6</accession>
    <name>A0A5B0RWQ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012954</name>
        <name evidence="3" type="ORF">PGTUg99_030370</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000136">
        <property type="protein sequence ID" value="KAA1129575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RWQ6"/>
    <dbReference type="OrthoDB" id="2499499at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138224" description="Hydrophobin" evidence="1">
        <location>
            <begin position="21"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12100" checksum="062B1F1C8B9AA4EA" modified="2019-11-13" version="1">MKFLAPVTALVVLIFGLVHGQDIILDKWCADNYGNVGKPWAICGQANWADNDPRTGILTWSVQPQSNCNLAGPIPFCCQQSIRKRVENNPADKPPQVDGAIMLDECHRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SH33</accession>
    <name>A0A5B0SH33_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_005322</name>
        <name evidence="10" type="ORF">PGTUg99_017113</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat UTP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000010">
        <property type="protein sequence ID" value="KAA1137281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SH33"/>
    <dbReference type="OrthoDB" id="1935146at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000908">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045161">
        <property type="entry name" value="Utp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359">
        <property type="entry name" value="WD-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="417"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="55"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="230"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="104"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1107186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1137281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="601" mass="67044" checksum="6F356B19306B8E7C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LHT3</accession>
    <name>A0A5B0LHT3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LHT3"/>
    <dbReference type="OrthoDB" id="10392665at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="365" mass="39895" checksum="A55B2DB508ABD1E1" modified="2019-11-13" version="1">MVVFQAVDLDLEAWVELYGYLQEEPPSLAIFPISPPQIHPIHSASMLPLRFRGRIKQAGLVPASSLILESIDHGFLSFRFNMSEFAPDDTAMTVDSPTQDTADPTDSTHVSSESAATLIPNIIEERDVAASFPIDPAVTSVSNVVDPNRPTHAYPSHQAAQVEEDEVPSYPPPPYVHAVRFASAGPYSTPATAGPPRSVLSHAPRRGYSTPRQAGSASPGSPTRPSVRRRLFPTSPAPTRAASPDDSPAPPTDPPAAPHPAPAGDPFDMDARFSAQFLAMSFYNTLLSVHDVLGDLPNDHPHFLRHEIMRAATIIAEDWFNYHRIHLPSQFAEPHSMGHLVANSVRFTLDDSCFFLKIPHHLSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LIV0</accession>
    <name>A0A5B0LIV0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027126</name>
        <name evidence="1" type="ORF">PGTUg99_004088</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000522">
        <property type="protein sequence ID" value="KAA1063790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1086015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LIV0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1063790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="73" mass="7879" checksum="647F73C48C4D4A2A" modified="2019-11-13" version="1">MQPNPTPGDLEPVAPGFLTNLLSLGLPPLLDPTSTCWGMQNPPPREISQNSLKTSMVASMPPYNRLRTALGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLC5</accession>
    <name>A0A5B0LLC5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UPF3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_001981</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RENT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLC5"/>
    <dbReference type="OrthoDB" id="18087at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12455">
        <property type="entry name" value="RRM_like_Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001086">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039722">
        <property type="entry name" value="Upf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112:SF0">
        <property type="entry name" value="FI21285P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112">
        <property type="entry name" value="UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF3" evidence="6">
        <location>
            <begin position="3"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="229"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="280"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="327"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="378"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="395"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1064408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="482" mass="51040" checksum="A6DB7C4C868F3DFC" modified="2019-11-13" version="1">MSERTKLVVRHLPPSLPEEVFWKTLSRWLEPIPREDGTLDPPRCTATFKSYVPGKTRRNKTKVEIPSRAYIQFATPDQVVEFHQGYASQAFRDSHGNVTFPKVEFAPYQKVAGPPKKIDSRIGTIDTDHDYQAFLARLNAPVPEPNTNPDKPDEAPEKVERPEITPLIEHLRNARQAAQEAALTAKQQRQAATSAKSTSGRSFAGPPQIMKRVATNNESNQPTGVSSRKSGDPASHDPPPHKIDSAPSTSKGVKQTGKSSSTAAASTGQTTSAGSNTKSRPNKPPKSKRAGRGADDQSSHPPATDSQGKSSASTQQPKMILQPPKPSDSNSEKQTSTTPKNPTQSPAKSGKKQGSSLAEQGQESQNGPTKPPNSTREGPGGSKKSGRGGKGGKTTTGPGNPTSQATGTSSSSSAPVAAAATQKPKNHEPAKTEPNSSETAAARRRLGNALAGITSKPDRPSTRKKQNSKTAPPSSSNPPPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLJ5</accession>
    <name>A0A5B0LLJ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLJ5"/>
    <dbReference type="OrthoDB" id="10269384at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022797019" evidence="1">
        <location>
            <begin position="21"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="145" mass="16914" checksum="EEDFDB07048BA4AE" modified="2019-11-13" version="1">MRFPVALAYLLFTALPIIQAINLYPEIRHETKVHFWDNGKPFSGYYSQPIRHKVGEKFYDTRCTVYDDGHVYVNNPFPVQCYAAVIQYPKKRTAPPMQGNMMPLGNQNMKVAIPLTYLKPEEGSTENQFSVVYYFHPNDYELLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLX2</accession>
    <name>A0A5B0LLX2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLX2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="13187" checksum="44ABD4CB786C451E" modified="2019-11-13" version="1">MHGEYDYPLQLDHVFTHDSYFGTWRARGRDALKPLLRPAEHQPQLSSYLSIFCLLTYQLNSLTRTRSNLLTVICTLLYSGSSFWLFFYSRLIFVVNINHTVLALRLAPYDISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPT9</accession>
    <name>A0A5B0LPT9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPT9"/>
    <dbReference type="OrthoDB" id="2506775at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="826" mass="94078" checksum="64E2401057C5D953" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQF6</accession>
    <name>A0A5B0LQF6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQF6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11180" checksum="EC29F1227DCE2F00" modified="2019-11-13" version="1">MMQDMRFEMGLTMFRAENSGTAEDEGPAGAFFADLDAAGLAAFALVFLASCSSSSFLVGRLNPYKKYLVIYIQLIIRPSFTDNKRSRSALAAAAFSAFSFLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQG6</accession>
    <name>A0A5B0LQG6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022980</name>
        <name evidence="1" type="ORF">PGTUg99_016666</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQG6"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="75" mass="8301" checksum="0A9F924BA3FA9814" modified="2019-11-13" version="1">MKPSIILASFEQWMKSLNLLDHFLSLGYSPQHANLLGSPPPPPPTPNQPNLLRDIVLHAPQKTNSVDATMLSLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LR70</accession>
    <name>A0A5B0LR70_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_050373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000192">
        <property type="protein sequence ID" value="KAA1066845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LR70"/>
    <dbReference type="OrthoDB" id="2497235at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11717" checksum="2484C7DAD9B1CE58" modified="2019-11-13" version="1">MHYVWWFLKHQQSFETETRVPFSLTPKTLQKLRGTQLEFEANQEPKTGFYCSSCGRLSIRIYWGSLVCSNEECQVGPFVVIGCGIKCAHGPSLSTLANLERNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRS0</accession>
    <name>A0A5B0LRS0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRS0"/>
    <dbReference type="OrthoDB" id="2507856at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023005399" evidence="2">
        <location>
            <begin position="24"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1066563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="516" mass="54216" checksum="47CCCA5280DC6879" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LTZ6</accession>
    <name>A0A5B0LTZ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010072</name>
        <name evidence="3" type="ORF">PGTUg99_015786</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000377">
        <property type="protein sequence ID" value="KAA1092180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LTZ6"/>
    <dbReference type="OrthoDB" id="412382at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.100.10">
        <property type="entry name" value="Glycoside hydrolase, family 7, domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037019">
        <property type="entry name" value="Glyco_hydro_7_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033473371" evidence="1">
        <location>
            <begin position="19"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="74" mass="8069" checksum="FEFAB5F4051B0B21" modified="2019-11-13" version="1">MDLFSLLFLLQLLSHSNTQQPGKTPENPASFIITDCGNGSKCKEVSGGLTIDANWRATYVMNQDQKNYCNDGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUI3</accession>
    <name>A0A5B0LUI3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006484</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUI3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="13028" checksum="A7DF059D62E96F98" modified="2019-11-13" version="1">MITGRHRSLLASFQGNLNHSIPCRETPTPSPNSNRTPHVLVDDSYRVWNEASCVQSQPPWARLWTKQLKTVLKGAMRPTIYAAQCPQHIQHLLKHLKKHQINHSCPQPIHYRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYZ1</accession>
    <name>A0A5B0LYZ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYZ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="8348" checksum="1108570DF21F6CF3" modified="2019-11-13" version="1">MLRLNNPIIICWYKGSRRNDHWGLFLQVQYKFLSESGTPEPDLVMRRLMPIETWSYRRTTDPFNIDICN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZ98</accession>
    <name>A0A5B0LZ98_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CHS1_1</name>
        <name evidence="9" type="ORF">PGT21_022375</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZ98"/>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF41">
        <property type="entry name" value="CHITIN SYNTHASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="473"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1069369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="519" mass="58487" checksum="FAF66734FDA70669" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M088</accession>
    <name>A0A5B0M088_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_032419</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M088"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09326">
        <property type="entry name" value="LIM_CRP_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000145">
        <property type="entry name" value="Cysteine and glycine-rich protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000001">
        <property type="entry name" value="Cysteine and glycine-rich protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF35">
        <property type="entry name" value="MUSCLE LIM PROTEIN MLP84B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="26"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="259"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="119"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1069736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="332" mass="36383" checksum="8F66D4B1D53A79D6" modified="2019-11-13" version="1">MSRTNTIHLNHRPSTNTKRAFAPATPKCPACLKAVYAAEQALGPGATPYHKSCLRCTHCKKVLDPFSILEHGYDPYCKICHSKAFGTKGVGFGNAVVGEYPTSPKLQSIVSPTIAFGSSLREERDSPSKPDLPFEPVPDHHHHEPIQAEPKPTNDQEADQEDFEPPSLSSLQIIVKPRPKTSETPEEVLHEDRTTVSRSSSTLHSTRQLSLKQEHPLTPPPKPNTSALSFPARAASSSPYNLRPSPTFPRPGFGADGPQRCPACTQTVYHAEQVLAIGKKWHKRCLRCTVCSKALDSNFNERAGKPYCTKCYDVHFGTGAHGFVLRAGFAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5F3</accession>
    <name>A0A5B0M5F3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RRP8_2</name>
        <name evidence="10" type="ORF">PGT21_011455</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of adenine in helix 25.1 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="8"/>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5F3"/>
    <dbReference type="OrthoDB" id="10258825at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016433">
        <property type="term" value="F:rRNA (adenine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1071556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="265" mass="29872" checksum="7099971C4E95F3D4" modified="2019-11-13" version="1">MKKPTNKLLFDTPGWTIPKENINSEKKAKRKKNRNKPTTERPSSDQQQEHSPSPKRLKNSHPHNNKQQQQQQQPTKNQPNETLHVGTHQLAKGLLNSNLNGSRFRILNETLYTSTGPEALKLFQSNPIEAAADDEEEEGGGEHTIRREENPNFEIYHLGFRSQTKHWPQNPVDLIAHELQQDPHLQKIPGPALVADLGCGEAPLAKLLCSSPSTSTSNQKNSKPIAHHNQFKVFSYDLVADREGWITVAECSSLVELPDISQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6H2</accession>
    <name>A0A5B0M6H2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6H2"/>
    <dbReference type="OrthoDB" id="2507165at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="131" mass="15081" checksum="4DC4FF81BAD5B919" modified="2019-11-13" version="1">MGLRDHLRPANQILGAYTSTMKVRLAYIRLEVVHHYLNPDPATNLSQWDIIDRRLEFLRRQSLNYKQAYARLIIKTDRELFGDFEFRDIPRDAIVLPSESQVQQEIGAANHVGPVGNGANETMVVDQDVFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAP6</accession>
    <name>A0A5B0MAP6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAP6"/>
    <dbReference type="OrthoDB" id="10271506at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022713624" evidence="2">
        <location>
            <begin position="23"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="226" mass="25166" checksum="7B5D4B9D18AB4B62" modified="2019-11-13" version="1">MSFILGPYCQLVLLQIMTESMALPTCGNEEIPALHDSQSWNWSNKDAEIDQYLSEPLAFSAASPHLQSSPVPAFDFQMHQEDMQGLSPGGLFPKKDGIHFHELLWPDLNDNIELLSPDVDLPHDLMIPQDNPSLDLPPKRKANELIPQKEYTESSSHLRNKLLKKNHNTDDISKIVVQSMLNTSSSLRSCGELGENVGGLSPAEIVQNKNERPRSLAPKSSHSLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB71</accession>
    <name>A0A5B0MB71_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB71"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="187" mass="20415" checksum="2946813BD9F582E8" modified="2019-11-13" version="1">MAWRTPIRTPGADWRAGLHAKEVWRAASHANLLQGSWPAVGVQACTPNFREAEVTPPGGTPPGERCTVQRKETLPLDEVHVPRPSEGNPSSGRDLLVIPRGVEGESDRHNEEDESPQDDGSEERVEETLPSTPDNQLQKGSLDQQQQHLSPNPIVNLLLRASDNPAILDSLVDKPDLENGHVELSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBL5</accession>
    <name>A0A5B0MBL5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022249</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBL5"/>
    <dbReference type="OrthoDB" id="10370556at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="104" mass="11974" checksum="E399DAC5C4B06C14" modified="2019-11-13" version="1">MPSKLELIHLDYEIWLLRVPPTTSNPAAEVVPHWALVFPEHDIPSLTEDLPESDFGRFQEIIFSHLGKTWDDLPLEPVLRELDAERKLSWQGIIKHVDPGSKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDH9</accession>
    <name>A0A5B0MDH9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_030941</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TBCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDH9"/>
    <dbReference type="OrthoDB" id="2500108at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF85">
        <property type="entry name" value="TUBULIN-FOLDING COFACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="5">
        <location>
            <begin position="8"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="6">
        <location>
            <begin position="199"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1075197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="28680" checksum="52470BD9A63230D8" modified="2019-11-13" version="1">MTTTNHLISIWVSSPDSYSERRWSPHLTISELRAKLQPITGIPAGSQRLALSLIPGQSDSTRSILLDNDQLSLLDFGVQEGATIDVIDTDPHAASQAGQYNDVSQVEKFELPKEEYEKRSDSLLAFKMRNKLGRFNDPAKSTQEDPSESATLSQTELLERYPLGSRCQVSSLTNPSETNPSRGTIRFVGPVEFNHKHPYWIGIELDEPTGKNDGSVELHRYFTCPPKRGIFVQPDRILIGDFPPIDLLQDDDDDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFY0</accession>
    <name>A0A5B0MFY0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_024777</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFY0"/>
    <dbReference type="OrthoDB" id="2496478at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="58"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1074924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="272" mass="30219" checksum="48E7765E5FF0EA45" modified="2019-11-13" version="1">MLRRLAAPAISDPQGTGIRIGLLKKIEMKGDDAMMAAIGKSAFDRYGQHAREQQVTTVFHLARGKNTFLLAGTGFGKSRIPEIYHTLLPKSANGVILVLNPLDALGDNQVLEKKKAGFSAINLTKLTFNVEEANNVVNGNYNFVYLSPEIFLNSRLWDQVYFCPNFQNRLALVVIDEAHIIYQWGLVESSSGKDKAVALGRIEDIAIFRPCYGKMGGRLLTRNGKPILLMSATCRLIAVEAIKKSLKFEDHNVCVVRAELTRPEIRIDSWDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJI9</accession>
    <name>A0A5B0MJI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_036074</name>
        <name evidence="3" type="ORF">PGTUg99_037315</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJI9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137261" evidence="2">
        <location>
            <begin position="27"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1091596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="250" mass="27153" checksum="D612B722214B84AF" modified="2019-11-13" version="1">MIVSCELGGVLLLACLCLAGLKPVFCSLWEASEDRASWESGESVGHLLDWTDHNGPLDIHEILCSTPNIPTTSTAVQSSAPKLERQQSPLPIEQTGQIFAVQANSSGGGGTSAGNLQSGHFQARKGKANMGSRSSTNEDPPGYTLGSHSDHVVPLLHFHPRPGNRDEVYPLAYTDHQPILENASNLETGPWSYDQILEDAHDYHDHLNYIGTEDLQNHSEDVHNIDQVLPSTLGIFDPEMLQSILSQDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKT9</accession>
    <name>A0A5B0MKT9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKT9"/>
    <dbReference type="OrthoDB" id="2504173at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179">
        <property type="entry name" value="PROTEIN CBG02620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179:SF2">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="338" mass="37904" checksum="B58ED89D0D67597A" modified="2019-11-13" version="1">MNIFTILVWLGITSNLASAFITFLFASGVINASVPYYLCVINLWAIQVSRIILIWSNQVHRQILRWGTFFWALFLTAAVFSTWIPARLRVSDKFIQIDSIWYRVTRVICLLTNAGLNLTFIFSVHKTLVANGLKKYNTLLRFNAKIMALSLVLDLVPLLLLICHSSLLYISFFPLSTMVKLETELVTNDLIVDVIKSEQQLTRIRDSRFATSPGCPTLDGLDSTINTPSTCVFSEEESTRSSKIKELNSLNLTLTDKWKLPHIGSSLASPPSLQIGKSVPARLFFTPDGKLDSSTQFGMFLPYASPIDSESGDWAHSRMSSGCSIETDITIDLWTRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MM42</accession>
    <name>A0A5B0MM42_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MM42"/>
    <dbReference type="OrthoDB" id="2513392at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022955168" description="Hydrophobin" evidence="1">
        <location>
            <begin position="20"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="15731" checksum="B7FF94AC02C71841" modified="2019-11-13" version="1">MRCTIAIALSVALLNSVSAETVSARGLDGLLGGLPIDGLAGGSSGNGGQLDCVKQNALLNVNALTSVHCTNNNGGGSGPAESFSRRGLDGLLGGLPINGLLGGGSGGDGGKLDCVSQNGLLLSANVLAKADCTNNAGGGGQQMGCGRSGGGYSSDSTTADCASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MN13</accession>
    <name>A0A5B0MN13_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MN13"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="253" mass="29938" checksum="C041F63F6B0EEDE4" modified="2019-11-13" version="1">MIFDNIQAPSKLQQNMMIATSIIESIKTDLKKFGVLSAYKGELREILGSISPPRDMKDSSNHDESAHSRSIIIERIPDNKPEYSESENLKDMVERLILLQISILEKRLSSHEHTVVNKISEFLYLVQREMFPGIMEEKLSQIQQDSKQFEDYFELQIHSYEFNKWWYIARDYSCKLAKTWGLNNSDPSKFDPKVYEEYGRKLTGIWDDFRKKYQSVFEKPANILRQFQDADHDIEELISNGEMYLINMKERIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MN67</accession>
    <name>A0A5B0MN67_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MN67"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="12642" checksum="8FF93FD556116AC5" modified="2019-11-13" version="1">MLLELKLSEIDDALLLLVCSFHEAQLSESKLVASTFMYHISYRHSSGPIHSKLAKRRAWLKVGLIGAESDAERLIGSFWNVTFNVLVLESAVTRKGNPICCPMIFISGTMQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNK2</accession>
    <name>A0A5B0MNK2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNK2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="74" mass="8399" checksum="CA89B71708DE57B2" modified="2019-11-13" version="1">MWAGHNTGSVKYWIGLGDTDTKFPRGSYRISPADPNHLINRDWIRNTIRSQYTVLGDISIQSGDRICYIVGASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSW6</accession>
    <name>A0A5B0MSW6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001232</name>
        <name evidence="2" type="ORF">PGTUg99_005085</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSW6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="90" mass="10015" checksum="D1191F078C30D2AF" modified="2019-11-13" version="1">MEDARRRRSRPRDLVVAVVDMGLVGRSESPRTGTAYTKRTNQPRPRALDCGRSFSSSSRDPNQNSTPERTETLLGPSQGASQLNQTTQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTJ5</accession>
    <name>A0A5B0MTJ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTJ5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="135" mass="14977" checksum="17060E24F214DBB2" modified="2019-11-13" version="1">MAVATNLARQGTDPATSIILPPLHNHPARLKLPFKEQRNIGAALNAKVTDHVTSWSSGVDSYLVHWLYTGEGRATWRNKRRLNPSLVVTARDRRNRLIVPSSLNAAWPAWLAASPTLNAQLIQYGTGKLFFLPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTZ8</accession>
    <name>A0A5B0MTZ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008765</name>
        <name evidence="3" type="ORF">PGTUg99_013558</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTZ8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="116" mass="12349" checksum="DCBBB517907E500D" modified="2019-11-13" version="1">MSAPREDSRQAASLQTATSDTSTRRPSPTSNGHTVPATRLRGDRPRAGGGAPQFPSAPAVVNHLSLGVFHGRWLPNAGVHRDCMSTGTYYAMCACGGVLRKETTRFGCADMFLATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MU59</accession>
    <name>A0A5B0MU59_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MU59"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="79" mass="9016" checksum="5DF9A61E652FCE87" modified="2019-11-13" version="1">MSKYDWPGEEPKERKHEQALQVDIVDPTGRADDRVSATCKLRSCPRPADAATRTPQWPMKKRPLPKPVRSSPTSFLIRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWZ0</accession>
    <name>A0A5B0MWZ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002578</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWZ0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="93" mass="10874" checksum="9D11DD75CC504B86" modified="2019-11-13" version="1">MQHNCYEGRCVDVEGEIPVNPRQEGSSICLHIKHTNHNSYLLNAFSHHAPDYHRRYSGLEPRPISDQQMLQALQEGLQNWRHEHIDDDDELSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXM8</accession>
    <name>A0A5B0MXM8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028209</name>
        <name evidence="4" type="ORF">PGTUg99_023911</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXM8"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026894">
        <property type="entry name" value="DnaJ_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44924">
        <property type="entry name" value="DNAJ SUBFAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44924:SF1">
        <property type="entry name" value="DNAJ SUBFAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="83"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="494" mass="54067" checksum="1E68B94FCC9A2BA6" modified="2019-11-13" version="1">MSPTIIPSRPREAYVAITCAHCKSHLEYLPPPSSKDSYLIQCHSCSKTFLPNSSAGTTTSSTKPSTKKTARRIGSDERPLETEYYDILGLTPKATPLEIKAAYRRMALKMHPDKNPDDPDAGEKFKSLAVAYNTLSDPQLRKKYNEFGKQQENDGGFVDPEAVFSTLFGGEKFQDIIGTISLGQEMKTAMQKESNEDEEQENDTGSQLVSASQQPPATSSSPKATTKPTLTPEQKAKRDAVAQAEAEERKRVRDARVTKLAEKLKSKLYLYTEQAEEEFDQQVMDSVKMMWEIEKESLAEESFGPELLRTVGSTYLAKSKRCLTATATGAWGGGVALVGGWFHSAKSTAHVLSETVGAVRAAYDVKAVFDELAKAEAEGGPGLSEERKKELEELAAKKGLRALFMGAKLEVESVIREVCDRILEEPGVSREVIRKRAVALGILGSVFETAKNKNGEDTLAELENGYVKVDPSKKKPATSPTDHQDSKAANATSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2R7</accession>
    <name>A0A5B0N2R7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PITH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_005328</name>
        <name evidence="4" type="ORF">PGTUg99_035714</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PITHD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2R7"/>
    <dbReference type="OrthoDB" id="2635at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.470:FF:000010">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045099">
        <property type="entry name" value="PITH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175">
        <property type="entry name" value="AD039 HT014 THIOREDOXIN FAMILY TRP26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175:SF1">
        <property type="entry name" value="PITH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PITH" evidence="3">
        <location>
            <begin position="22"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1083537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1093980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="214" mass="24160" checksum="BF7683985A6960B3" modified="2019-11-13" version="1">MANPHQHHSCCSHEAHNQDDHHIKPGEGNQDFLFSKIALDQVTGYNVEPNRPAKSCIKPWDDRMDDQQFTESDMDQQLIIQIPFTGSVKLRTVIIRTLPGQFRPTQAHLFANEPTLDFDTLESRKPTQILDIPETTEVVEFPVRVAKFSSVTSLSIFFNSTTPGADKSQVYFLGFKGEFTNLSRKPVIAIYEAQANPTDHQKIKGLNDSVNHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5B9</accession>
    <name>A0A5B0N5B9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
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    </evidence>
    <sequence length="3672" mass="395233" checksum="9048ABCD5D1CCD43" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7S6</accession>
    <name>A0A5B0N7S6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003636</name>
        <name evidence="4" type="ORF">PGTUg99_002225</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000250">
        <property type="protein sequence ID" value="KAA1118429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7S6"/>
    <dbReference type="OrthoDB" id="1043111at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039540">
        <property type="entry name" value="UBL3-like_ubiquitin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13881">
        <property type="entry name" value="Rad60-SLD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="194"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1118429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="235" mass="26669" checksum="802E18B6E65892ED" modified="2019-11-13" version="1">MTTTTTNRSSTRTSFSSSQTIIEPERQQELQLQLNNNSSSSSSTTNSQVEHSRIQVQQAHQPTERGEAERRNGEEDADEEHERRLRLIEVNRTRTRLIEEELEEEPPPLAGLPGSAPPTAVLTNHCQPPLPIKEPLPETIIPLEILLSPINQKKAFHFDHLLSIAELINHLLNNWPADWSQPGSTIPHHTHQFRMVYMGRFLDDQETLNSLGKNRPIIIHLVIKPDEGTVEEEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBT9</accession>
    <name>A0A5B0NBT9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_009587</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBT9"/>
    <dbReference type="OrthoDB" id="342064at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039727">
        <property type="entry name" value="SE/Ars2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007042">
        <property type="entry name" value="SERRATE/Ars2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021933">
        <property type="entry name" value="SERRATE/Ars2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165">
        <property type="entry name" value="ARSENITE-RESISTANCE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165:SF0">
        <property type="entry name" value="SERRATE RNA EFFECTOR MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04959">
        <property type="entry name" value="ARS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12066">
        <property type="entry name" value="SERRATE_Ars2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="696"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="255"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="521"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="614"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="739"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="880"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="304"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="623"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="900"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="934"/>
            <end position="948"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1086717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="981" mass="108512" checksum="96B15002DABAFCF3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDM5</accession>
    <name>A0A5B0NDM5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCT-beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CCT2_1</name>
        <name evidence="10" type="ORF">PGT21_014069</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDM5"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03336">
        <property type="entry name" value="TCP1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000002">
        <property type="entry name" value="T-complex protein 1 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012716">
        <property type="entry name" value="Chap_CCT_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02341">
        <property type="entry name" value="chap_CCT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1086863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="523" mass="56402" checksum="EA4155AE64DAE20B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NE99</accession>
    <name>A0A5B0NE99_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NE99"/>
    <dbReference type="OrthoDB" id="2497888at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="807" mass="92047" checksum="AAC65C96F7DE34CD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NES4</accession>
    <name>A0A5B0NES4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NES4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="134" mass="14593" checksum="A83E643EC197A264" modified="2019-11-13" version="1">MVCEMCISSRNHLTDNTEVSPAPDLLGGTSDKLGRGSKLSVKGGLRGPLGRMNEAARRPWLDKSTTKPMSGLGQQAANIRNGLSTLRDRLVRADYCRRYAHSNSNQAKLKSLALNNLNPNAFLRPPPPLVDVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGV2</accession>
    <name>A0A5B0NGV2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex III subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">QCR8_1</name>
        <name evidence="12" type="ORF">PGT21_021106</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with cytochrome c oxidase (complex IV, CIV).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UQCRQ/QCR8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGV2"/>
    <dbReference type="SMR" id="A0A5B0NGV2"/>
    <dbReference type="OrthoDB" id="6683853at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.210">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004205">
        <property type="entry name" value="Cyt_bc1_su8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036642">
        <property type="entry name" value="Cyt_bc1_su8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119:SF2">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX UBIQUINONE-BINDING PROTEIN QP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02939">
        <property type="entry name" value="UcrQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81508">
        <property type="entry name" value="Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1087069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="56" mass="6519" checksum="C0EF6970C576071A" modified="2019-11-13" version="1">MKNAFRGYLFQGYRRLAAQVPYWIVPFGVAYGVIKWADADNHYRNTKAGHRDGKFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJK1</accession>
    <name>A0A5B0NJK1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ornithine decarboxylase antizyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_019338</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimer, and targets the monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with ODC and thereby sterically blocks ODC homodimerization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ODC antizyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJK1"/>
    <dbReference type="OrthoDB" id="5959761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008073">
        <property type="term" value="F:ornithine decarboxylase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.60:FF:000008">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002993">
        <property type="entry name" value="ODC_AZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038581">
        <property type="entry name" value="ODC_AZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279:SF10">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02100">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0688">Ribosomal frameshifting</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1089475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="254" mass="27718" checksum="5C87997844A3C6F5" modified="2019-11-13" version="1">MEEMPPDDYSEGGRDRHVRDVTAPNFPNPSSGPVFVKFPSSTTVSPITPSSSPTGIDCHSLAPTPPSTQNRLNNNNNNNTNKMMMASLDFKTHRPSAHNPRPNPTPLLTPCSSSDSLSLSSNGFNNTRHSTPAILTKIFPNGIPDVEAVHDISTQLPNALKGAIVDRDSGIRSLYILGLTSSVTIDRQVRDIVVRVLDLADEEIEADQVFFALEKDHPMFRELLQGLLYVGGNVVKNAQHQPVNKHLVLVGIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLN3</accession>
    <name>A0A5B0NLN3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLN3"/>
    <dbReference type="OrthoDB" id="2500663at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="57754" checksum="055BFB12BC020213" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPB5</accession>
    <name>A0A5B0NPB5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CsbD-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPB5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8460" checksum="F2396A2BEAD66234" modified="2019-11-13" version="1">MDNGKKADFCGNKEHLVGNTKDTIEAAKSLIGKTKDLIGETENSIGETKYSIGEKKDSIGKKKDLIGKTKDSSKDTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPR2</accession>
    <name>A0A5B0NPR2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_004504</name>
        <name evidence="8" type="ORF">PGTUg99_031877</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPR2"/>
    <dbReference type="OrthoDB" id="429183at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38483">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38483:SF1">
        <property type="entry name" value="ION TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="40"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="191"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1074246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1090534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="233" mass="26799" checksum="FB91AA41BBB7BACB" modified="2019-11-13" version="1">MKSQLKKQTNIASPSTRSHYHLSREEWIKGIANRFVFSQIYLYLYLSMAILSLTTVILSLLSECPTLSFYILEIIVNLAMILEVVIRLIAFGKQFWKSPFNWLDLMITLFCLVTVLVIFSQGCKAKKEEVFDTFLLVIRNGIQFFRLALMLRRSGKNIFTTIQPIDLEGAIQHTDAHSFFLDLDEEEEFLRRPSHSSPRNHNNNNNKNTPLGAADSAAVPFLQSRSSLDDQDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NXS7</accession>
    <name>A0A5B0NXS7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008174</name>
        <name evidence="3" type="ORF">PGTUg99_021018</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NXS7"/>
    <dbReference type="OrthoDB" id="64220at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="383" mass="43394" checksum="E2189F8E87775B2D" modified="2019-11-13" version="1">MTLCPKSQRLPPQKASDVPSPGRYNPLLHDPSQDPCRKDPLEIYKAPRNKESDPDPDTFGLYNSDQEHYDNIRGSRPRSRALSTPATPSKHHKELLRLETKLSEYESNITHLSETLQKSEQTTKEIRNALNKSENDNESLRSEVSRLKKQLHGVAGLHAQVIEPETEHDKSKKQRENDISNFKAELRSAETRASQSSKRNQSRMSQKDEFRLLTSFVLPAKVLQSSDSGVRSLGLPTLDLHLARLSIGHQAIAQAIPESWCAKESAIDPEQIVKALNSTIQSHREQSPASLNSLRVRLTVGPSQAVSLVTSTMPAIALTKLLVSIDDRPTTYEGEPFLLTKTTYRSHYDHTRSRHGLWNHISFLLLVYNRSRHEYYKKETLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P035</accession>
    <name>A0A5B0P035_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_024229</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P035"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022958784" description="RING-type domain-containing protein" evidence="5">
        <location>
            <begin position="28"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="64"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1094533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="209" mass="23676" checksum="20A30A9175E9DFB0" modified="2019-11-13" version="1">MNLPKYTSKLLSINFVTLICLIKISLQVFIPHESSSGVEDVSESVGQVNEVTSNPRFVSSDTNCAICIEELQRDKDNIQIGTLEGCGHSFHRTCADPWLKSKGTCPTCRKTYYEGYSYKDDPKIYQVNVQASQSIVQPVLDRVRVLLDNIDLEGLRDLFQTNYNPERHDIQLPPNLHDQTPQELAGNLDETELRRLLAIISGYIGNRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P059</accession>
    <name>A0A5B0P059_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P059"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="51" mass="6143" checksum="AAB8CF5DBCAF7689" modified="2019-11-13" version="1">MVKALKTLILKRKFFTITLRKLLIQELQGFCVEKQQLIFKIFPQKGFHKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0V9</accession>
    <name>A0A5B0P0V9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">GPI inositol deacylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">BST1_5</name>
        <name evidence="9" type="synonym">BST1_2</name>
        <name evidence="10" type="ORF">PGT21_019172</name>
        <name evidence="9" type="ORF">PGTUg99_014761</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000479">
        <property type="protein sequence ID" value="KAA1071197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0V9"/>
    <dbReference type="OrthoDB" id="348976at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050185">
        <property type="term" value="F:phosphatidylinositol deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006505">
        <property type="term" value="P:GPI anchor metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039529">
        <property type="entry name" value="PGAP1/BST1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495:SF7">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495">
        <property type="entry name" value="NEGATIVE REGULATOR OF VESICLE FORMATION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="8">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1071197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1094364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="469" mass="51691" checksum="8E5C24821026D46D" modified="2019-11-13" version="1">MLTMPNYINQTVNTILSLSSPHSIPPITFESGVERVYDQINCIPGVWSPIDHLAILWCNQLAIVLAKTLLEITEPRKQNQVRSVQGRLAILKSHLILGHGIEPFSGNDDSALSKFHLESLPTNQKAYHQFNPSPVAAPGPDDRADPPQSRATGSTSNHQSNSDEFRNLSSSSVVFTSRSFLSFGLFGAATQILPNQSSLVNEVQLPDLVSSLVAYKLDFRSGSECERGSAFAPLMRQETPLLGESKFHPHLRSAILYTHLSNAYTPSRKMDREEAQKKAGVRLTFWTHPMVCGGSSDRAIDEDGNQNGLIRKCTTSNSVVSDCIRLSSGRLVSAHPGIPVEGTGREQIVYIVWRGAFTAGEDLTGHRRGSTDPIVRPSDDQLYRRPPDWIGFALIDPPRLVGDLLLGVSQASFFLLDWILVLVSLGWLVAVYVLLEILFRVSGLLWIKLVGSAHLSLSRSRKQVRQVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1J6</accession>
    <name>A0A5B0P1J6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">O-methyltransferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_004435</name>
        <name evidence="5" type="ORF">PGTUg99_036135</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1J6"/>
    <dbReference type="OrthoDB" id="2410195at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="207"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1083558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1093958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="460" mass="51134" checksum="2EDF73BD8AB4B230" modified="2019-11-13" version="1">MPVSAARQLVELIYQAVDDIEADVESRLPGGKLAELNSPIVAPEDGLEMSPKRREALRTLQAATHQLISTLMPSGLYVLNSYASVFQKTAIDTVVGARIADLIHSIDPDSRMGGVHVEVLAQKATMDPQKLTHILRFLALRNIFCELSPNRWTNNRHSFPLRTDSSNSILNALGHMREETSLPALVELPKLLLDKQVDGAFSKDPHQSAFQKYYEPGCSLFEFLAKSQGGYRSERFGKAMLEMAQANGSGMSVYKAFDWKKLGPRGTLIDVGGGVGAAAYTLATYLPGWKVVVQDRPEVIKDGKKNHQRKASTANLEFEEVDFFEEQPRHRSGRADAYFLRHVLHDWPFKSCVEILTHLRIASKPTTCLLICETALNPPLLDPDSPILSNGGMASVSTYTRNLAMMTLLNAEERSQEEFLEILNQSGWKLQSITPLATLADYFIIEGVPDPAWNQETRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3G2</accession>
    <name>A0A5B0P3G2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3G2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="11435" checksum="94C55D578C29194A" modified="2019-11-13" version="1">MSAYGRQTRARDRKRAGPFDHILTTVFDGKVPTYLHITTWSRKKRVDRACCDGHQQPPTNPKRKDTQPNICQNPPPGTVKSVSMWNGQSPPNPSASSFLCGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P418</accession>
    <name>A0A5B0P418_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P418"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="64" mass="7075" checksum="089C861109146CE3" modified="2019-11-13" version="1">MLSASAARIPHAPKQTGQSFGPDALSPPQRELGQTSHTPECDNTQVEERFLEWVSINQQLSQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA49</accession>
    <name>A0A5B0PA49_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023657</name>
        <name evidence="4" type="ORF">PGTUg99_036171</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA49"/>
    <dbReference type="OrthoDB" id="2285229at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF65">
        <property type="entry name" value="EXORIBONUCLEASE CYT-4, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G01550)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="2">
        <location>
            <begin position="620"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1117136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1199" mass="134845" checksum="D02BBC1202EAEB75" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBI3</accession>
    <name>A0A5B0PBI3_PUCGR</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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            <taxon>Puccinia</taxon>
        </lineage>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1097970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBI3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="9037" checksum="7FDB621C4D26D7C0" modified="2019-11-13" version="1">MRCTSMTGRKSFRLMRYDVPRRPERILPVTLEWMASSVHGRCDRDLVDQLRTSANGRHAPSPVEKLFGGRWFEKIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PDW6</accession>
    <name>A0A5B0PDW6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_48</name>
        <name evidence="2" type="ORF">PGT21_000900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <dbReference type="OrthoDB" id="2507470at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
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    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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            <dbReference type="EMBL" id="KAA1098912.1"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEM0</accession>
    <name>A0A5B0PEM0_PUCGR</name>
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        </submittedName>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="73" mass="8225" checksum="CD64FD9850C1643A" modified="2019-11-13" version="1">MKFGFPRLPPHKSLRSASTPRNSEVRSRSVAFPPGSLKEWNRDEQARPTKLHHGPSSTQTELNIAIAYRQAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0PEN2_PUCGR</name>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
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    </dbReference>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="51" mass="5549" checksum="03D6B2E7EDB0ECC5" modified="2019-11-13" version="1">MPRGRTQPLAQSSGGSSVSQQPVENEPEEVKFSPLTDQEELGPENCSKFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PF72</accession>
    <name>A0A5B0PF72_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PF72"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="12756" checksum="7D48A6871E95A55E" modified="2019-11-13" version="1">MGGGVGALATVVLVKAIIQNERDVLISPHGNGVFTGGTAASQARAISWGIFSRRLFLFGQKYSAVSWGFLVGFFLPIPFFIAHKYWPRYSVFCWRNHACLHRANVTVLGAQVSGTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PH22</accession>
    <name>A0A5B0PH22_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation initiation factor IF2/IF5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_001669</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PH22"/>
    <dbReference type="OMA" id="CMREGNK"/>
    <dbReference type="OrthoDB" id="10255414at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="5">
        <location>
            <begin position="243"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="33"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="155"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1100925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="375" mass="41375" checksum="86CF5F82120DB690" modified="2019-11-13" version="1">MAEELNNPPVEQAEEPLFDATLKKKKKKKAVAFDELDSQLDNAAEQPTSITTSKTEEPVSILKTTEAEPSSKAPEPAASGDLDFSDLKRKKKKKTVTLDLDEDELNVADEKDKSASTATAAEDAAAAEEFADLKKKKKNQKKAFDLEAFERELAEVEGGINKSEAKEGDPSGADGDDPPEDGLFGQADDEAIGESEKSKAQMAAEKKAWLKEPDRDYTYDELLGRFYQALYLSHPSLSGGGAKKRYTLAPPSVHREGNKRSIWANVAEICKKMHRQPEHVIQFLFAELGTSGSVDGNGNLVIKGRFQQKQIENVLRRYIVEYVTCKTCKSPDTLLEKDNRLYFVTCESCGSKRSVSAIKTGFSAQIGKRKSQRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJ86</accession>
    <name>A0A5B0PJ86_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_006440</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with coenzyme Q.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COQ10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJ86"/>
    <dbReference type="OrthoDB" id="292693at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901:SF10">
        <property type="entry name" value="COENZYME Q-BINDING PROTEIN COQ10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="SPERM PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="4">
        <location>
            <begin position="38"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1101086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="207" mass="23581" checksum="E04C044F7B623233" modified="2019-11-13" version="1">MFHSRALKLSSQIANNSPRKFSTSTIRTGSHQLLKNLPYERSKIYSVIVDVQSYPEFIPYCLSTRVIGPVTNLDQKKENHKDIIDQDSQSTLINHQFEVFTRIGYKGFETDYHSQVICEPFESVRVIAKPSSTFTNLTTTWTLSDPPCSSTSTTTTSNSKPSTQVALDLSFKFKNPIHEYLLTDQAIWKKFSTLIIKSFEDRLASLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PL50</accession>
    <name>A0A5B0PL50_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PL50"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="322" mass="34523" checksum="B0BA91CEAE674B29" modified="2019-11-13" version="1">MGPNPTIYVELPWPTSAAVSSPDFTMSPPVVPPPSLPLLSQLSLPPSFKQHREPTPAIPISPSSTDSHDSSKSPGLPNGQSSALVSPGVPDPIPSEDLQDEVKLSSDPSLRSVPNVDPVGQLRPTEECLVSFPIDLQDEVKPSSDHSLNSAPDLDPIGQLWPTEECLVSFPTDLRDEVKLSSDHSLHYIPNVDHIGQLHPTEECLVSSSIGLQDEVKLSSDPSLCSVTNVDPIGQLRLTAKNLVSFPIDLRDEVKLSSDPSLCSVPNVDPIGQLCPTAERLVSSSIRLHKEATLHSQVQESVFSSQASSGPNNPMSSLSHNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLM8</accession>
    <name>A0A5B0PLM8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLM8"/>
    <dbReference type="OrthoDB" id="2499434at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="288" mass="31513" checksum="41F71C06732406C2" modified="2019-11-13" version="1">MSSVPSDLPGDLSSESSVRSDKVSRFPLTNAAILAAGMGTYHPFTFQDMLEYSSLDTGSPLSLPGCATLAGEVDPTFEDPWMAMKRKAILSVLNRPSSYARGLPAHMPGSSSRSVRDLKMKGKQLVRDCSPIPANSPEHPVSPKTRAPTDRVVHLISRVGPAYQSPYPPSKSTSGEPSSGDEESTVGPMSPRDNAPPAFKRSRMESDRPFLPLNTTDYLDLTPSQLDELLHEGKEFPTRFVYNAEQLNEIAHCCLIDLIDFFNFYAPGSPQEASAQDNQKTRAQNHMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNB1</accession>
    <name>A0A5B0PNB1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNB1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="176" mass="19637" checksum="95EEC22A85F89CA0" modified="2019-11-13" version="1">MNLFCHPGSGAGPRWWSGWKFEPNEVGAPLAASVPHLLLQTTATPHPGSDARAPQSFYQKKKQEVDPERWNAAIIFQGVDTDYPAPRSNHQALFAPLKSTGHSNLSGCVNHRHHLSPHHVYDLALSSGVDIIDFRDCLDLQEIDQSVLRKRSNPGSLRHPIEFHKPREGDTPIRCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQ91</accession>
    <name>A0A5B0PQ91_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQ91"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="246" mass="26910" checksum="7FE3472A2DF3C1DE" modified="2019-11-13" version="1">MLKHKTPRRDHTLSQKRFTTYDHESMTVSQASKVSVRGVIVHSDKAFGKTGSRPLVTNSSIPIGNSREVVSRSKPIKTSVPDGHGVSSDWTKRPVSLFPTGARHRAQIAAGDLDNLDLADTARVLRAVSLKFGGSWCIKSCQSSLTQSTIRQLNEFIVTAILKHARYLGLKLTIATNARALSLSQYSYLKSDKTSGCSLQSTRTAFSDDSSSLAASFAGLGRVRSRRVRYGVLIGFHTSISMSIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRM7</accession>
    <name>A0A5B0PRM7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRM7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7844" checksum="B94F1C71D156C843" modified="2019-11-13" version="1">MAELQIEHTPASYSTHHSHHKKTSKAEGNLLRVTEATKKHDFSPEGQPKSNRTDSAVSYRCELYWCTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUI7</accession>
    <name>A0A5B0PUI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027873</name>
        <name evidence="3" type="ORF">PGTUg99_004470</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000119">
        <property type="protein sequence ID" value="KAA1129851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUI7"/>
    <dbReference type="OrthoDB" id="3227170at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="432" mass="48059" checksum="E8457F8CCBE14B99" modified="2019-11-13" version="1">MGLRQLPSNVFTLFDEYPLMKKYRNRRIAPTILLISILLLSSLVIFNVITQGKSLRYKTFLSSQFFNQTVFATGDNVTCQPTPMKLSESFFTVIPMKDAPRVSVSLRGYQWTIYAVIPPGGPVSENTTTGFLYSGEKLNCTSEYVHHTYTYDTGNFKFNSCASCLLSTGFIIKICTLYDSATPKLPKSVQLLYNNIPDLGFQSLDKIYHDMTIPPGSLPVSAFPPEHSETQPSSVANKTIDWSQVLQLRSWLGNMSLIPEPANKSSKEKIMLNNFTVVDSFFDAFKATDGQNSWSGFEFSVKDIGTVKPLGQVYNLSGIPEVWAKVFDYSLSMNNYLMHAANEDLSGANGKQIGVNYLCTNTTKEWQAVPKVVSVTVGSTLAVFSACFTLLVAVARRLDNFSIKNAEMHQLAESRTQSTLKVDDEEVVRLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PV33</accession>
    <name>A0A5B0PV33_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_027207</name>
        <name evidence="3" type="ORF">PGTUg99_007147</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PV33"/>
    <dbReference type="OrthoDB" id="2503485at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137851" evidence="2">
        <location>
            <begin position="16"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="280" mass="31038" checksum="A02BA307154F192D" modified="2019-11-13" version="1">MWFSLFILILATSQPITILTKTSKNSDFALSGHSFSTDMLHMSHSAMHGPVSKKRLWRPSSTQKNGPTRVSKTRDLPSHNKLESRSSHKHTRRADWYKQDRIYAALMDCLAGIQKHAKNMKDIASNVSESENGEQLAWALLKELKAILDLCNECLARIKRGDFPLPSSKPGFFEHATLIDICRVIADILTEIRLCFQQVNELAAKFPIIHNICGDTLNQISGAVANLIIASSGQLGNIIRLIARIFASTPGFFKDIGFGFGNIPGIIAGSGGSREFDRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXQ7</accession>
    <name>A0A5B0PXQ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXQ7"/>
    <dbReference type="OrthoDB" id="107110at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="796"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="802"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="899" mass="100699" checksum="D35A936E3AC1CB27" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYS3</accession>
    <name>A0A5B0PYS3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025722</name>
        <name evidence="3" type="ORF">PGTUg99_020496</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1120934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYS3"/>
    <dbReference type="OrthoDB" id="10020110at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="790"/>
        </location>
    </feature>
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            <begin position="828"/>
            <end position="852"/>
        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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            <end position="110"/>
        </location>
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        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <end position="482"/>
        </location>
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        </location>
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        </location>
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        <location>
            <begin position="1067"/>
            <end position="1080"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1119" mass="130463" checksum="37A8DAACF134DC7C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4L2</accession>
    <name>A0A5B0Q4L2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4L2"/>
    <dbReference type="OrthoDB" id="2502444at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="44620" checksum="2B16DE645DD14B80" modified="2019-11-13" version="1">MGHQMIARQPTNINYGSPQLQTSLSQPMLRKMLLLCFTSLIMAEVTLVAGMEGIKAGAKFSTSDLKPEHTASYDIKAGKAQEKIMGPTSKGNEKVGYAYPYYAPASPPIPVYQPERKSSLGRLASKLKNYYFSFKMEFERFCSFKWLKDLFRTNHHNNYPPPVFYPIYHLQPPFRPHYHHAQFPEPAWGEPLHSNIHHHAGQENEVFSPSDSSVQSTDRKIKEVVEKLRGTKVTAEIPSKNYWTPVEHLPEHYRTTDYIKEKNGLFRALAHFAYEDQEQYGRVRDELIAYIAKHPGQFEKYLTKKGEHDSIEVDDHLYRLSNGESIDGAELAAFSELYKLNLVVVSKKGDSVAAHQHVVDPSNNEFSGVILQDGHYQLLTHSKNPTIKIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4M2</accession>
    <name>A0A5B0Q4M2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase with role in protein import into the ER</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">KAR2_1</name>
        <name evidence="2" type="ORF">PGT21_002267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4M2"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="177" mass="19384" checksum="AE867796C7EC4BBB" modified="2019-11-13" version="1">MLRKGPISPRNKSALPKTRSTDSNQADQNLGSVIGIDLGTTYPGVGVQERGRVDYFIQLWKRKHNGEDITTNLRTMGQLNRDVEIPKCTFSSHQSAHIEIESALTHPFECDPTQISPATSSPRPRPTTARCSSGGGGGTQAVGFSRQGTFIGLTSFTRPELTQHPASLGLLRRYHIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5H3</accession>
    <name>A0A5B0Q5H3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5H3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="84" mass="9516" checksum="A12F8D7D29CB4BFF" modified="2019-11-13" version="1">MIEDENEDKLISETDNHDELASNINLEENNMITEEDTDPEENNMFDNEDFDGLFTNAKSDETISKTSAPLSCTKSNMNVADCDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9Z3</accession>
    <name>A0A5B0Q9Z3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9Z3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="241" mass="26026" checksum="2BC10C879F5E1671" modified="2019-11-13" version="1">MMCNKAGEGGDGSDPFRKKNGLDDRKESLLVIKTRLPPSPTGQEGFPGVIETFSTGSPRAATAGGRTRTRQSTGFRGLHRLVHGCIARLSRGAHEEACRAAVKESAAALKRSAHGVELRQRTQAKHPGECLTAGPQGVGNDPSFRDQIGSELSGPTSVGPGCKWIEGRPSWNQRGRRAHVLPTKSNLVLWNHRQKKRSPRETDDEIGLTEHLQSRSRGFHGVGVSRGIHIPARQEAPLGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAC5</accession>
    <name>A0A5B0QAC5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAC5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="291" mass="28088" checksum="14FB35DD179006C0" modified="2019-11-13" version="1">MALPDSNTDPLAGSQKPPSANPTDPTTSTTTTNTNPSSPSPSSPSPSSPSTSPPGTGSPTPSGTNPTNNPQNPTPSDKGSTSDKNPSDKNSSDKNPGSTPTDPNNPGTTNPGSTTPPSDKNTGSNPNTNPASPTASTANPTSSAPNGAAAPASSSTPQTAPSKPSTADPLAPSPSPSPAASPSGSTANSPGTSSSSSKPDSVQSSSPVPFVTTKSTPNVPGGVSTITTTISAPVGPATSTPHRNTPPSTSSPPPDNSSGLSGGAITAIAVVAGIIGGYRPQQVKTQPCEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB31</accession>
    <name>A0A5B0QB31_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QB31"/>
    <dbReference type="OrthoDB" id="10347490at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022824981" evidence="2">
        <location>
            <begin position="24"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="104" mass="12196" checksum="1364E90BE937FA5B" modified="2019-11-13" version="1">MLIALNIVLVIVEFFHLIPSSSTKVLHRRMDRFPDDYRPYQFHNGRETMVHDAARPIVKEIDRAALEASEASEDTRWTTGYRHQETESRTTKKVEENPRGRNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBQ7</accession>
    <name>A0A5B0QBQ7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_030503</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBQ7"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1110736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="282" mass="32561" checksum="6C53D9CC8B9D1209" modified="2019-11-13" version="1">MYRFIKRIGNGAFGEIYLGIDLQTNQEVAIKVESIETKDPQLRNEAKIYSIMKGAIGFSSLRWIGIGSRYTALVMDLLGCSLDRQLSARGTFSLKTVLMLADQMIVRLEQLHSAGFVHRDIKPGNFVMGRGSRSHIVHLIDFGLSCRYRGDDHQHIPYKEGQPLVGTACYSSICTHLGIQQSRRDDLESLCYMLISFLTGSLPWLGIRAESKLEKHQIMAERKIRTPLEKLCQGLPHEFACFLNYTRQLRYKDRPDYEHIRRMFKSLFLRKGYRMDLVFDWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFH2</accession>
    <name>A0A5B0QFH2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFH2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="236" mass="26965" checksum="6B789828619DC49D" modified="2019-11-13" version="1">MKNQFEWEVEKLIEEIVLLKNTILGLTPGFLKPPKYTRTKLLTRRSVFPLQYNNNSNLALATHFRIIKEKLLWGTSKRFQTAQLLPLTAPDGMLQVSKDFGSAKDNEKFSKHRGVIDDLIGLVTSIELYKVAGVCPMSQHGASPELDLAKQLWIAYGSKKTAVLEMARNWQGFGLIFQDIMEVMMPSKSIEKRQSQMYSDLFEAYQQAIHTKDCCCKLTSSKNTVIDRQIIDFEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QH54</accession>
    <name>A0A5B0QH54_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QH54"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="106" mass="11575" checksum="5B258D0BA2D8273E" modified="2019-11-13" version="1">MNPPNPPTPYKPPRTQPKTSGPGNPRIASPSQQIGGRLHIRPLTSLTAGRHHIRPSLTAGQHHTLTAKLTHHSPANNCESIELIRLTSPLDHTQLFSAFDLENQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMV7</accession>
    <name>A0A5B0QMV7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP-dependent DNA helicase sgs1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">SGS1_38</name>
        <name evidence="1" type="ORF">PGT21_012793</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMV7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="229" mass="25492" checksum="2DB912D781E39E94" modified="2019-11-13" version="1">MGQGDPAAIVQMVGHCGLDWNVGMGLLFMERMCKNGKNNLSDFPIGAYQDNDTRMDALSVTQCCLRIALTLDNKLGYIPLLNDKPQVQAEQAQEVKLGFAKCKCLNFLPVDSKPLSKDHTDRVKPPLYRTGINHPLPNTHHTQTLQSTHSNNLLLLNTQVSYPEADRIRRRLEELEAKLKSAHVKRVMCGQAHTARLLWKGQIADDIEAMRDSLASGHMQITPQGDSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNX8</accession>
    <name>A0A5B0QNX8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="1">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_027563</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNX8"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF6">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="3">
        <location>
            <begin position="245"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1114931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="388" mass="46323" checksum="475193219C1011C8" modified="2019-11-13" version="1">MTGRQPGNHPQYPYYPTQNSNPNPYPNRWPPPPQLTREQHLENQRRSEERRRRYQESLPAPHEPKQERKRQKELKQQQEKEERERKQQERQEQQKLEKQERQRQNQLKQQQEKEERQRKQQLEKQERQHQNQLKQQQAKEERQRKQQLEKQERQRQNQILQHQKQEQELEKQERQRQNQILQKQKQEQERLAREEARKPKPPPPPPYDKELDEHYYHVDQLLDRTGPLANSSFVTGAAPKDLMRKTCKILVIGAGGLGCEILQNLALLGFADIHVIDMDTIDISNLNQQFLFREQDIGQPKAEVAAKFIMARVPQVKVTPHYCKIQDKDDAFYMMFNLVVSGLDSVEARRWINATLVNLVNPENPESLKPLIDGGTEGMFMRLSFRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPC2</accession>
    <name>A0A5B0QPC2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dihydroorotate dehydrogenase (Quinone), mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">URA9_3</name>
        <name evidence="1" type="ORF">PGT21_022100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPC2"/>
    <dbReference type="OrthoDB" id="10395724at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="60" mass="7078" checksum="7881422AEB893D2E" modified="2019-11-13" version="1">MVNWSQIVGFQVRKIDLQPLIQDELKHMKTASYEDQVKQIKQTIQFIGQSCSLFYPRPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPJ8</accession>
    <name>A0A5B0QPJ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033781</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPJ8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="130" mass="14118" checksum="8740B47C6BA99D47" modified="2019-11-13" version="1">MTLPQRLSQKQAALSALISNIVGVLKDIAITPLDPSCGAFRMRTKHPACHQNRLDQPKVHELRSAPFASIAVPPSRWTTAGRAPHPQLAELLPPTSASLPASWASRIKMNVLNNVVEGSKGILIQQDKDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPS3</accession>
    <name>A0A5B0QPS3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPS3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="13745" checksum="3A68AE5CB9E60CE1" modified="2019-11-13" version="1">MEATKERPKLFGRTPFARLLFNLRVKEVQLKLLPISNRNIEQLHLCNISQAVLRACGFSPYSVDYIGTTVLGPAQLRSGLSPKPSEACAVLTTRVLEITLDRRPSLPLSLSCSKGSCDEIGAVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QV28</accession>
    <name>A0A5B0QV28_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SGF29 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_031256</name>
        <name evidence="8" type="ORF">PGTUg99_020414</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QV28"/>
    <dbReference type="OrthoDB" id="10265994at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20393">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20394">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037802">
        <property type="entry name" value="SGF29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010750">
        <property type="entry name" value="SGF29_tudor-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047288">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047287">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539:SF0">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07039">
        <property type="entry name" value="SGF29_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51518">
        <property type="entry name" value="SGF29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SGF29 C-terminal" evidence="6">
        <location>
            <begin position="266"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1098237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1116793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="407" mass="44710" checksum="A4BC26701112B554" modified="2019-11-13" version="1">MRRRSEGCLFTNQPSSSSSSTFGTNRKFKEPSDKHPTGEHTLHKINVRLQPYSMSTRRRNPNGNCSSNLHSGEDASIWRDLYQRLNQLIAAQDRTSQSALQLSKLLAPHESISASLDTLSRHVTDELGILDVCLERLGILIALREAPPDPPLQGRSTKKRRLEPASSAGSCDRGVSPLASTSTSNSQTQATSEQPNADSPASRISSPVPLSSLPPMFTQTQTRSQVGSRSSTPISDGSTSKKTDSSSTMSARPTSLRIDRRVAFRDQLPLLPGRLVAFKCPRKPSDHPAEEHWIMAKVVKCISGDRNRYEVEDVDEEGRKGAKQFSWNTTLKSIIPLPVKDHPDTYPPAVLPPGTTVLGLYPDTTTFYKGKVKSGPSDRARRYKVLFEDDADTGALNVGMEHLVPIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX68</accession>
    <name>A0A5B0QX68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QX68"/>
    <dbReference type="OrthoDB" id="2501485at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="426" mass="49500" checksum="47208E4C059D0C1A" modified="2019-11-13" version="1">MKQTRSNDSLTTTSEICWNQKQESQSKTFNQILNAALQPHSLIFWLNLPNRPVKWHHLPLQVGIKLHSTSHSRYRLNIFHRVNLVHLLVREHSPLKISFVIINLRQLLLHLLNSQHLDANPARHSRHTNIPSSSSQSLSSKIIGSPDDLSRDLSRRIELVHDGKRLKGHSSDHMEDHHGHVPIPVYKLKDVVPHKKIIQPNRRIYRMAFDEPPLLTFQLEKVQTLLSESDLPPGNYESKQIGNNDRARICRFCNENGNLIMTIRIFDFHQNTVKMLMLRRSMSSLMQWIHISHQAFLNKLEIKNDQRGAYELKFWDWFFSETFTPTNGLPALGKVTEGQATSYDPKNPFGTLQEHLIDYLSQDAPSDHALTKAIIILGIWYKNFHPETWNGLDDSSYTSWIEDAVNLFLSERRKKRRTNPAILCPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXM5</accession>
    <name>A0A5B0QXM5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030688</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXM5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1118067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="140" mass="15925" checksum="9823EC4770302BE9" modified="2019-11-13" version="1">MTAICYYTTGAIERSPNVPKTLAKILRSSNCWDHEGTRPRWLGPSPLSIDNWLFNSPRTLSTKAIQARPNSQAKLLRLPSLRNRWASQHCTAAVIGIISLKSHPCTLVPQKMLDNRSSSRFSFRLDTRIPEKEALNMKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYX0</accession>
    <name>A0A5B0QYX0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal RNA methyltransferase FtsJ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_032469</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYX0"/>
    <dbReference type="OrthoDB" id="289250at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF12">
        <property type="entry name" value="TRNA (CYTIDINE(32)_GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="5">
        <location>
            <begin position="21"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="187"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1118153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="229" mass="25237" checksum="646AADEF6D0F1E2D" modified="2019-11-13" version="1">MGKCSKDKRDIYYRLGKSEGYRARSAYKLIHLDEQYGLFNNPSQAVDRVIDLCAAPGSWSQVLVKKLNETRSAEEEPAKIVAVDLQPMAPIDGVVQIVGDITKLETATSILSHFEGQKADLVVCDGAPDEAFVVCRNYAPPDGFVPDMSHPLLMNPATRCQSDEGTCQDLRRILDFVACGDLSAWPDSDMNYPPPSPGINEGPEDTNPIAPPTEPPYKEWLLKKSRGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R124</accession>
    <name>A0A5B0R124_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_017738</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R124"/>
    <dbReference type="OrthoDB" id="10560663at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022667105" evidence="2">
        <location>
            <begin position="20"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="12427" checksum="C10B88CCFF5F3A5E" modified="2019-11-13" version="1">MKSTTASIFLALLLPAVMATTHTLCYNYFLNKDGCVFSAAASDQRCPAPVKEHSTPVQAFDMTSGSNMHKRSENRRLERRYDTTRPSFAVAGGEGVSHFSLSCMARHAAGYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1U6</accession>
    <name>A0A5B0R1U6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027953</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1U6"/>
    <dbReference type="OrthoDB" id="10269375at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="83" mass="9534" checksum="511D68FA8A55E501" modified="2019-11-13" version="1">MFVLQSIQDDNDRRVLLMIVLIPALLHRGFTKIPHGLEEMNDGWLLAPKITPPKFRSAFRLCMTSGSGRNIEQLESRRYPIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2V5</accession>
    <name>A0A5B0R2V5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2V5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="92" mass="9554" checksum="EF626222176A944A" modified="2019-11-13" version="1">MTEAALITEKSNRGAKASTQHQKAQVFCMTHGGAFLLRLANGGRPTVVQPSPDNGPTKPPGSPSGDGWSLVGDSAWLERAWLQPSSDAAVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RIH9</accession>
    <name>A0A5B0RIH9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HHT1_14</name>
        <name evidence="12" type="synonym">HHT1_6</name>
        <name evidence="11" type="synonym">HHT1_7</name>
        <name evidence="12" type="ORF">PGT21_013382</name>
        <name evidence="11" type="ORF">PGTUg99_009587</name>
        <name evidence="13" type="ORF">PGTUg99_034292</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000474">
        <property type="protein sequence ID" value="KAA1073850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RIH9"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000001">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 8" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="10">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1073850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1107417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1124775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="135" mass="15252" checksum="187B66A50D7EF43B" modified="2019-11-13" version="1">MARTKQTARKSTGGKAPRKQLAAKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQESAEAYLVGLFEDTNLAAIHAKRVTIQPKDIQLARRLRGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RKJ4</accession>
    <name>A0A5B0RKJ4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_034669</name>
        <name evidence="6" type="ORF">PGTUg99_030815</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RKJ4"/>
    <dbReference type="OrthoDB" id="1742084at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398:SF4">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="503"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="144"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="252"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="407"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="472"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1106417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1126396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="596" mass="65843" checksum="ACA0932A752ED91C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RWP8</accession>
    <name>A0A5B0RWP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027682</name>
        <name evidence="2" type="ORF">PGTUg99_006703</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1128934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RWP8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1128934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="71" mass="8025" checksum="9C5012F600670CB1" modified="2019-11-13" version="1">MEMIGWAIENQFALTKEANRLDVALATQQRSAVLAWVEPEQSQTICLVIENRLASTNKANRLNVAFETFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S3X7</accession>
    <name>A0A5B0S3X7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036993</name>
        <name evidence="3" type="ORF">PGTUg99_013847</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000080">
        <property type="protein sequence ID" value="KAA1132472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S3X7"/>
    <dbReference type="OrthoDB" id="2505289at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="440" mass="48258" checksum="EF85CBBAB4D6050C" modified="2019-11-13" version="1">MGGTTTGESPRNETRIETDELIELEMTINNEEEDGDGDGDGGEGEEQDSSNQQLVKPPRRPPTFEAAFSQARSLTNHGRVNYQQALINNNHSSRGSLDSLALLPPPPPPAQAENNLQSLRPTNSSIEQLHLTLPGQHDPDTHFLTSLVDSETTADQLIFSPHHQQVSLSPPTDSLLPIPRSPSPLLDEPNSSPPGLYGSSPPPDRSPSPPSPPAPRTVRVSPPKNPTGPPRPPPLKLCLPPRPRRPAIETSQTPYNSPAGSNPSTPLISTSNLRPVSNQSNLLKRTLPSSSTDQHQNQNQNQNQTTTAKKQKINPLFLVSSTSKTPAENEKLKAWKRIQLLRAQRDQPQHLSSSGSTSKNSEELERLEKSTNSNSLGDTLSSAIQLRNQAQEYLDLIQKTQDALGEELVRVQLEESVLRHVRGIIADRLIKNQEWNNQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S7B9</accession>
    <name>A0A5B0S7B9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033083</name>
        <name evidence="2" type="ORF">PGTUg99_022021</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000072">
        <property type="protein sequence ID" value="KAA1133305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S7B9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1133305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="81" mass="9341" checksum="5EA5561A929645B8" modified="2019-11-13" version="1">MQCTQPIFLATRDFGIGITECRGSWSPAQDQCIHRYESIARTTHWLNEFPSIPPAVHNVTYIIHLQALFFDGRGLRETFEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SDW7</accession>
    <name>A0A5B0SDW7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012765</name>
        <name evidence="2" type="ORF">PGTUg99_022101</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SDW7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1136020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="60" mass="7262" checksum="8C77C4974E87A263" modified="2019-11-13" version="1">MLEDMNYNAHEALIALANGWHQEYFPRWIRMDFFGLWDERVTPQHARVSLTQNSAEMFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SIR8</accession>
    <name>A0A5B0SIR8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005102</name>
        <name evidence="4" type="ORF">PGTUg99_018519</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SIR8"/>
    <dbReference type="OrthoDB" id="2505543at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138390" description="Secreted protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="18912" checksum="EE8EAAC0EC9E5D0A" modified="2019-11-13" version="1">MHYSLFSVSIYFIGLVTTSISSPYPPPIGGEKTLKCTRYSNITGPNTTCNDDPHQVCTGGCTGTVVASQCTKEGDPSDHSEQQMCTISYSQPSLERGVCINDQGTFSCQGIPQGEATCSGCAFVANGSDAGVAQISNSSHADSVLSGTKTPLSLSTAALLTGSRLTLFASSLFGLIGILALIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKT1</accession>
    <name>A0A5B0LKT1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032841</name>
        <name evidence="2" type="ORF">PGTUg99_006027</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKT1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="67" mass="7010" checksum="2BF9434358C39502" modified="2019-11-13" version="1">MDNNISQQKLHSPETHTPPNIFHSVMLTGTEWELGVVQGTDGGDVGGSNTDPLSKAKGDSESSEATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLL7</accession>
    <name>A0A5B0LLL7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001753</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLL7"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF3">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="244"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="694"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="860"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="438"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="705"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1064404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="887" mass="98172" checksum="5441103822AC39F7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMX1</accession>
    <name>A0A5B0LMX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_014158</name>
        <name evidence="3" type="ORF">PGT21_014167</name>
        <name evidence="5" type="ORF">PGTUg99_013379</name>
        <name evidence="6" type="ORF">PGTUg99_020043</name>
    </gene>
    <organism evidence="3 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000314">
        <property type="protein sequence ID" value="KAA1104971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000278">
        <property type="protein sequence ID" value="KAA1112405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMX1"/>
    <dbReference type="OrthoDB" id="2508479at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036136993" evidence="2">
        <location>
            <begin position="16"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1103316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1104971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1112405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="106" mass="11000" checksum="4D6A1064D3DB048E" modified="2019-11-13" version="1">MKLIFLSLLVGSLGASPAIRRGAPNLPPEGPKNLVPYEIKGDGYPEIDPSYIPTSQTKAPVLICDQQGKPIVTEQSSTHGAGSVAAPTKTEVQKSADGQGSASSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPG2</accession>
    <name>A0A5B0LPG2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Plasma membrane ATPase</fullName>
            <ecNumber evidence="10">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PMA1_3</name>
        <name evidence="12" type="ORF">PGT21_021343</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="10"/>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPG2"/>
    <dbReference type="OrthoDB" id="2501025at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000008">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="681"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="749"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="792"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="821"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="854"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="11">
        <location>
            <begin position="48"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1066099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="959" mass="104479" checksum="FA95473D0337BCCD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPS9</accession>
    <name>A0A5B0LPS9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004249</name>
        <name evidence="3" type="ORF">PGTUg99_020502</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1080002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPS9"/>
    <dbReference type="OrthoDB" id="2506958at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="425" mass="48651" checksum="84B161D7DB70E46E" modified="2019-11-13" version="1">MTDSTNKPSEEPKKLSKEQQLRQQGDLVIQGYKDLADKTSSFLHKHNELLYLTRSRPASFQNAVSIDQAHLKKGLLVQLQSSLVPQLQQQIFTTLQLTNPYDLTKDPGLQFKCILEVQAELNPILDQIFSATDVLCPGPLPSPSIRTNDQYLKELKPFRIRALYDHLTGCFLKQIFKVCNDSYDLIQQLKLSTKKYEGEVDLASARYYLNAEGDYCLQLIERAINCLEGSEFDVVQVDWRYNQSVINGHLRELLRRINPTTPLDEQNSMPGPQTLSEPVVNLTKSVLPVIKLCRLFFNKLSERGMNRKRLPLFTTMCSYQLNLISRLAGDIAGKLDRILSILRAADTTLNENIIRSLTKTVKALETHFETPFLLILLHFLPIIPDTVGNPVQNYFKEWFAIWNTQFTLAIKNLLNVIESYKGDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQ19</accession>
    <name>A0A5B0LQ19_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQ19"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="237" mass="26367" checksum="4C3CC96F58CCF969" modified="2019-11-13" version="1">MPCQSRRTAFHSGAQSKLYKPLTARHKLTYDNLPSSSSQVFRNQYSVSFATLFTVYTCPLQLSTSVCHSPSEKEPLAFFAPTTRHLVEIRRNQPDRLKDAIDRDRSIESSQHRSGNILAPPLEAQSLGAIRGWNDFIVASQAKAANQPGPDNYASVPTWYCGKLLTSLKGGDLPHLYVQLLLFTHPELVEYIILHILRSHPSQLFQIHESSVQLDEGDSSPSPVVLSGSPFPSNIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LR81</accession>
    <name>A0A5B0LR81_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GET3_28</name>
        <name evidence="3" type="ORF">PGT21_000511</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000190">
        <property type="protein sequence ID" value="KAA1066855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LR81"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="916"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1098"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="525"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1101"/>
            <end position="1124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1066855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1140" mass="125477" checksum="BCB98150E382C274" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRE6</accession>
    <name>A0A5B0LRE6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RlpA-like protein double-psi beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_008933</name>
        <name evidence="3" type="ORF">PGTUg99_027787</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1067031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRE6"/>
    <dbReference type="OrthoDB" id="406505at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF24">
        <property type="entry name" value="RLPA-LIKE PROTEIN DOUBLE-PSI BETA-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1083847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="25183" checksum="6CF98776CC4BF690" modified="2019-11-13" version="1">MVSPRFQLLIVASAAQILNSFQYALALTNDPSPDQFSRSSKPDFTFPADSESEKPWPLAGSMTRDETSFHDQPVAAFPISGVGRKTNNTLVERGHEHHEGDTPIKNLLGHGMGLGILGDSNEEDDDAEFTGGWATYFTQNQIAGACGKVHQDTDVIVALDYRRYGALNKVSKYCGKKVRITSGDKTIEATVADACPTCLNHNCLDLSEGAFKKLGVTVQEGMKPISWKFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LW68</accession>
    <name>A0A5B0LW68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LW68"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="156" mass="17086" checksum="E16B1D50CE806C7E" modified="2019-11-13" version="1">MGRRPPRRAVIGKGSTASGQAGIPRPSSLLPITTQPPGGTLSYRKKRLLLGEAVGQLGVSTDRWSTVYRLPRRPFNLADLGRLPEKTTDDLRRSWMLSYAVVGPRREESRPADGLQQSPLFSSPAGWVVIRVPNNIGPVFLRSTSFSSSGLQYLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYW8</accession>
    <name>A0A5B0LYW8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYW8"/>
    <dbReference type="OrthoDB" id="10295906at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="401" mass="46122" checksum="E4A8AB8658CDB61B" modified="2019-11-13" version="1">MLDLENTARLKSDVCKAFRSLSSFCCPRSMTPENDDYEPTISTDQIHFLTSLLIDMRTALLPSLRQCLNDLVLLLHPSHFLQDPQANLRATHEITSQLADIMGKIRFAVCTVTFAQLDVFEFEDHLHGIAKSHRTQPLLHELNMLMSHDLRVLFRKHVKFIQGWKSLKEERDGTYSSQRRDMCPETKLTSDHINKIVQSLEKSDFGLIQASWQAVVNSYDHGFADLIEYIIKSGSVTNSSIAPTTTRVETPRSRLRSLLQAAIPFFKLGRIYLNKLSSNPISRPPFTIQSQISSTELNCVKDKTYMFSLLLLHFNKTLVDLTHSYRLHNYQSSLKDKFENILKYFNASVELFSRHSVPLVSTHQLPLPRSNTMFKNHFSLVIDQFRLAAQNFSMALERSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZK2</accession>
    <name>A0A5B0LZK2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_021339</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZK2"/>
    <dbReference type="OrthoDB" id="10258882at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019452">
        <property type="entry name" value="VPS39/TGF_beta_rcpt-assoc_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF27">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10366">
        <property type="entry name" value="Vps39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CNH" evidence="6">
        <location>
            <begin position="43"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1069333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1128" mass="125122" checksum="B92F8AFB1DB1953C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZT1</accession>
    <name>A0A5B0LZT1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028212</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZT1"/>
    <dbReference type="OrthoDB" id="107110at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="815"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="863" mass="97948" checksum="3420D5C8031281AE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0D0</accession>
    <name>A0A5B0M0D0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005142</name>
        <name evidence="3" type="ORF">PGTUg99_036580</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0D0"/>
    <dbReference type="OrthoDB" id="2501650at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="286" mass="31948" checksum="15F2722D6E00575D" modified="2019-11-13" version="1">MKHKTSPAASASLLDQIDITPEEREFGWNGSLSGVRGLSEQQARRILHFENSLRQRLEIDRTEDHERDATSGNEFGKLTSGTTNEPLEVVAARHCRATCTRNPFSPCILKPRASLPEYQRLASSRLNRSLRISSSQSSFLRFKSIKRSKSEESSWSSESDEFSYLIAREKIGNSDQTSLSDSYTRSQGSNQLVRSRSTKTGLRGIIRKLGGALSLNPSRCHRKAHPDDKFPEDGSYLCHLSHDMVGSASPFPMRPPLYLGSTHPSTPLNGEWIGVSRKVVSESNRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1K1</accession>
    <name>A0A5B0M1K1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1K1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="97" mass="10901" checksum="DCBD6ED45F71F4D6" modified="2019-11-13" version="1">MGLRLMIHEYCPRLLRSVAAVNTYRWYLAYHDMIAHVAPRARTVGPAMAMKQCSADSRCNLPVLFPSTPESAGRGFQPMHIRLSISSTPGPFLHFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M216</accession>
    <name>A0A5B0M216_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M216"/>
    <dbReference type="OrthoDB" id="2506803at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="261" mass="29194" checksum="7CAB5241B6D8491A" modified="2019-11-13" version="1">MQQHNPQDTLVDSLQPANPSSLAIAPPAYFSMPNLTGLPAPIDNAEPPQDTTSSPTRVLGLGPPVIFECGIKFTLYCRERTRRNSTKWVAVRSSPNLSVTFNTRDNVNWVGFQHLVADESNRQYNNIARMILDGTNSSPPTITWRAYVIKNREWTKSNPQPLTDQASFLRWIFAINESKQAKGGVILEMSNPRNQQTRARAEDLLAMTVRRMDARQRGPSAGNVVNAGSESEHDVDGPEFSALDLRIEEIYARHGVNTEYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M4S2</accession>
    <name>A0A5B0M4S2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Medium-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_009951</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M4S2"/>
    <dbReference type="OrthoDB" id="9988775at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070991">
        <property type="term" value="F:medium-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000010">
        <property type="entry name" value="Medium-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000011">
        <property type="entry name" value="Medium-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF2">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="11">
        <location>
            <begin position="130"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="10">
        <location>
            <begin position="244"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="9">
        <location>
            <begin position="352"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1071501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="506" mass="55279" checksum="EBF40226CC58A8B4" modified="2019-11-13" version="1">MTPWDVRICHTYPWPVVGGWICCLVLSPPGVRVTTSGTTVLQIPSQLLLQFVFPNTFCASHRQQDSHQRRTRKEKMFTGRARLRLPAASSRPRSRLPLTSSGPLKTFRPYSSSSSDRDLLAGPVNFALSADQQAYQDLARRFTVDHVIPVAAEYDRTMEYPWPVLKEAWKAGLLNTHVPEEYGGIGIGLLESALISEEIAYGCTGIQTAFEASGLAQAPLIVAGSDELKRKYLSMLIEEPLVAAYCVTEPGAGSDVAGIKTQAVKTGNDFVLNGSKMWITNGGKASWYFVLAKTDPEAPVHKSMSGFIVDANSPGVLVGKKEINMGQRCSDTRQITFEDVVVPSSNLVGAMGEGFKVAMAAFDITRPLVASGAVGLAQRALSEAVKYAQTRKTMGKPIIEHQSVSHMLAEMTIGVEAARAMVWKSTFIKDTGHRNTYYASIAKCLSSEVAVRNAGMAVQIFGGAGFNTEYPVEKLYRDSKIFTLYEGTSQIQRLIISKFLPTMYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6A4</accession>
    <name>A0A5B0M6A4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6A4"/>
    <dbReference type="OrthoDB" id="2506553at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="335"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="426"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="520"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="629" mass="64705" checksum="A21BF5B84C376683" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8H9</accession>
    <name>A0A5B0M8H9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_034644</name>
        <name evidence="7" type="ORF">PGTUg99_007261</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000245">
        <property type="protein sequence ID" value="KAA1120288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8H9"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051356">
        <property type="entry name" value="SOX/SOX-like_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789">
        <property type="entry name" value="FI18025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789:SF2">
        <property type="entry name" value="FI18025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="125"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="125"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="196"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="415"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="338"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="490"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="511"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="542"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="587"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="599"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="615"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1072463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1120288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="642" mass="69446" checksum="DE0BF059BBD85B5C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9C9</accession>
    <name>A0A5B0M9C9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031891</name>
        <name evidence="2" type="ORF">PGTUg99_000550</name>
        <name evidence="4" type="ORF">PGTUg99_001552</name>
        <name evidence="6" type="ORF">PGTUg99_002324</name>
        <name evidence="5" type="ORF">PGTUg99_033714</name>
    </gene>
    <organism evidence="2 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000476">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1081238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1090265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000171">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1132186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2509374at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1126704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1132186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="225" mass="24294" checksum="B86ED6DBF6E78D1C" modified="2019-11-13" version="1">MFLPKTAGAADALTGGVQGLRAGETLDSGVGAGKGLSNVVDSTNALRGTGTETLKPQDLMTMRGGNEAKSLPKTEDASNLDKTLTKAEDSSKLDQAKADEVEDLDEPRILGRQGKIEEIEKKIKWYAEHMGTSAPPELVKQLEGLKNGEPIKAIKATPAWKPGLQTIHSQKLLEEISETAHSLPAAQKLSMWKKLLTHLLEYFRNSEGILPALVMKLHLKTPPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAK1</accession>
    <name>A0A5B0MAK1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2504034at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="315" mass="35496" checksum="7468A4636C27AC94" modified="2019-11-13" version="1">MSLTSYIPAYKNLREMESIINDLTSRLREEANAYDSEKLRGFSASSESSLLQSAQKLGPLRVELAKNLRRGLFIYCIDTCLLVVVGQTLAQFQLAVEFDREFPSLQIYVPLLWISFRGLYEGSAPPENVEAAPSLIKRLKKNPLRRKLNRDRRRLVCHALCVYASTALHLPPLIILLTRNMNNFVSDISRREISYHGLNDPLAVLANVILLILNINSRQLIRARMEASRLNPQPPARSPDPNTEQRKAISNSLNDRSSQRCSPPPPDIHIALNNLEENVPYNEKTASWCTCAAPGDLDRKSPLVISDPHANETDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAM0</accession>
    <name>A0A5B0MAM0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAM0"/>
    <dbReference type="OrthoDB" id="2509372at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="182" mass="20840" checksum="4A2DC84E913A2E07" modified="2019-11-13" version="1">MTTLTRKEPAAPTQGKSKKTKDDPPPNTEKDTPMDVDKIESECSEATPKRPSTPEIRILTKEEQIRLRVREHVSLWKQCQLATREGPTAKLRALLTTAQESQKTLQKLIDNDEIESYVKGWNPWDEKKIHFPSPPKKNLMKFKKNEPGKRQSSSSMNFSDPVKWKKATDLLQIAAGLYQNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCB4</accession>
    <name>A0A5B0MCB4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine-zipper-like transcriptional regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LZTR1_12</name>
        <name evidence="3" type="ORF">PGT21_021042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCB4"/>
    <dbReference type="OrthoDB" id="2505591at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="159"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="352" mass="40116" checksum="31A8364389A2D9F2" modified="2019-11-13" version="1">MFRNRTSTSPTQPPDLLASTKPHHQPLSSLVKNRFRTSSKKRVRPGKDIKRWALNKKKASAPLEGSSSINRTEDAETHDAPDSDPTTNNNKHSQKNQEQHVYFYFVPPHQQFDPNPSEVITTQKTLQHYHHLTHGTCIAAPRGGPAFCKVKYVPFASMSPEQIQGWEKLVCHFFDRTNYVEAVKNNGPQMAGDMWADGWRKCSKKESFGRYCSVGKLVKMMRRANYNPRDEATSIKEASDFIAIQLQHLAPGVFESYRETLISKKLPSMAHMEYPLPYDALDFASFLTFTMYNFHNGPHKDSDANNWTLVCWIPIFNPRTASDDDPILADEGFDMLGVQFSHTPDSSWSFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGG9</accession>
    <name>A0A5B0MGG9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGG9"/>
    <dbReference type="OrthoDB" id="10685467at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023069556" evidence="1">
        <location>
            <begin position="19"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="667" mass="78256" checksum="9991FC620D1AB1DC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJW0</accession>
    <name>A0A5B0MJW0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000985</name>
        <name evidence="3" type="ORF">PGTUg99_029897</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJW0"/>
    <dbReference type="OrthoDB" id="2506685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="714" mass="82363" checksum="051236852C7960BC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLJ8</accession>
    <name>A0A5B0MLJ8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="1">2.3.3.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_010517</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLJ8"/>
    <dbReference type="OrthoDB" id="418791at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054692">
        <property type="entry name" value="LeuA-like_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911:SF1">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22615">
        <property type="entry name" value="IPMS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="3">
        <location>
            <begin position="33"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="2-isopropylmalate synthase-like post-catalytic" evidence="4">
        <location>
            <begin position="271"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1077512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="352" mass="39137" checksum="2C4B1288BED7AFCC" modified="2019-11-13" version="1">MAKQDNNPNPTLVRCGSSRWKPISGRPNDSRTKDQIFFNLLLKCGFKEIEVGFPAASDTDFDFVRKIAKSRQPNLDDVAIQVSVDICIYMLLGIIFQASKAKTIDLAVRHAELVRQLSDDAISRGDRTEWQFQYSPETFSQTEPEFAVEICEAVKKAWLHGKDMKKSHPIIFNLPATVEVSTPNVLHTHNDSGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYSHGLAPNLDFSDIKEVIDIVTECNDIPVHPRHPYSGDLVFTAFSGWHQDAIKKGFAIQNANSHWEMPYLSIDPHDIGCDYEAVIRVNQTMLPLNPELNNNDGHKRTAHSAHLSTLKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPY1</accession>
    <name>A0A5B0MPY1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026094</name>
        <name evidence="3" type="ORF">PGTUg99_009589</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000081">
        <property type="protein sequence ID" value="KAA1132399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPY1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366165" evidence="1">
        <location>
            <begin position="19"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="135" mass="15265" checksum="D61C495A78FABF73" modified="2019-11-13" version="1">MIAFQLIFISVRFASITAGLDRAIESLNAESTKIDQEESISAIMNEMNIKGKSHKHISRASSSLGAFIYPINESVEDKPSKAEGCSRNIKHCNLDQNQIYLNKKYFKEISKCKKNICKIKTRFEENENCDRKGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVF3</accession>
    <name>A0A5B0MVF3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002416</name>
        <name evidence="2" type="ORF">PGTUg99_012660</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVF3"/>
    <dbReference type="OrthoDB" id="2507389at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09276">
        <property type="entry name" value="Rnase_HI_RT_non_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="70"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="287" mass="32243" checksum="3788ED5ACB586DE8" modified="2019-11-13" version="1">MSASPSTHPSPIHNILDKHLISNYNLSSIETIYPHIIDPWEDFSIPISNLKTKKEEIKSKVQLQINNLKSKSEHLIFTDGSNIPEIGSASAALLDNTTEFSCRISDSQKSSAFEAEVQAISIGLEIFINKFLNSPSNVSSNIAPINIFCDSQSTLFSIANPPLPKSYQSTFLNIFKKLKIISKLCHIPIALFWCPAHVGIPENETVDKLAKAATLSSHPHHLQNQIQLLSNTQQLARSFFKFNKTKKSIIRNKIRLSFPPLKIFEQLDQLERSTGSIIYQLRNQFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MW75</accession>
    <name>A0A5B0MW75_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MW75"/>
    <dbReference type="OrthoDB" id="2518661at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="125"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="215" mass="24980" checksum="CF0C2F7EF89F0FC7" modified="2019-11-13" version="1">MTSATLIVVPDEASHILIPPQRKRAAFHARLQERVIGVDMSTLKSIRWQRLSYHPKTRLYPTVPFDKKTDKPLCHPTPDEVSKACELVDRKFFLMNFGRVVLVDPNDEDSVIAVMEFTPWDQLTETDKENIKFISTFLHQSKEFVNPVGSSTRSWGGKMWGIGWRKSQDFMQKFGRYIKAFPPSKMEKFDKLFQQSKLLGKILGDYYRVASIMQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWC4</accession>
    <name>A0A5B0MWC4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010437</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWC4"/>
    <dbReference type="OrthoDB" id="10444325at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10538" checksum="BFA5D9A5EA1257B8" modified="2019-11-13" version="1">MDWKLGWRIVASDWARWPGSHHADGQVSLNFGASSWIAAAERQGCNQSFLETETPRGCSPGSHSELVLGSSPTGTPLLVPTYKRRMSILIRLKTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MX23</accession>
    <name>A0A5B0MX23_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MX23"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="151" mass="16364" checksum="2A9DF16E956066B1" modified="2019-11-13" version="1">MGWPLLTLIGHWSRFKTLTCDLEHPCRTQAWVLHGCSTGGGVPVQPRARGSILSSAGRLRAPPSLSYSQPPSHPVDQPTDHPTDQPPAPEPHRAPDKHPVSTQEPPTTPPRHQTPAHALISADPLRSAPIRSDPIRSGAENLPPGFEFCQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZU6</accession>
    <name>A0A5B0MZU6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Smt3-specific protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ULP1_1</name>
        <name evidence="8" type="synonym">ULP1_3</name>
        <name evidence="7" type="ORF">PGT21_005692</name>
        <name evidence="8" type="ORF">PGTUg99_026366</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZU6"/>
    <dbReference type="OrthoDB" id="2496817at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.395.10:FF:000024">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="122"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1082505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1133597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="339" mass="38826" checksum="14B5BDE09B4C32ED" modified="2019-11-13" version="1">MAFRKRELRPDEPEDEDDDDPIDMLSSKSRTSSHMKVGPSRDPSQSLLNPITDLPPAEIEPVTPASPDLDLLFSRFGQLRLAKTKSKSHQRKWPATLTPAQQIVVDEGLRPNNKTVYDLPGASCGIKELQKLSSPPQWLNDEIINFYGSLINLKSHDQISSKALNVHCFSSFFMSQFDLGGHSGVKRWTRKINLFEKDLILFPTNLSNLHWVLGVINNRKKRFEYYDSLAGRNSDVLSKLRRYYQDEWQAKKSEDVDLTEWSDYQPKVPLQSNSSDCGVFVCQFMYSLSQNLIDITSQEGKDVSLFDFSAENMPYLRQKMVLEIIRKSFLPDITPESPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N043</accession>
    <name>A0A5B0N043_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008169</name>
        <name evidence="2" type="ORF">PGTUg99_019196</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N043"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1133501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="120" mass="12875" checksum="229A0FCC29333255" modified="2019-11-13" version="1">MWEEGDQHLLPSRRGVGYTLGREGLFCSESGPAFPFRSPALKLKCHFCAHMQVVPIGLCDSTVLERLNPALDHLGITTVRSVVDSLDHMAGGPLSSRGIVQNGSANAKPRPSVAGRAVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0U6</accession>
    <name>A0A5B0N0U6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033966</name>
        <name evidence="4" type="ORF">PGTUg99_013386</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0U6"/>
    <dbReference type="OrthoDB" id="2498589at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137328" evidence="2">
        <location>
            <begin position="26"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="652"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="748"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="707"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="756"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1066580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1081818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1161" mass="131525" checksum="0074AFAA35C2BD91" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2I4</accession>
    <name>A0A5B0N2I4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein MSH3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 12">DNA mismatch repair protein MSH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">MutS protein homolog 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MSH3_1</name>
        <name evidence="16" type="ORF">PGT21_010090</name>
        <name evidence="17" type="ORF">PGTUg99_008200</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="17 19">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2I4"/>
    <dbReference type="OrthoDB" id="121051at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.110:FF:000010">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000037">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000004">
        <property type="entry name" value="DNA mismatch repair protein Msh3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF122">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein MutS core" evidence="14">
        <location>
            <begin position="544"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="15">
        <location>
            <begin position="881"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="143"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1111"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="27"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1082674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1133428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1157" mass="130017" checksum="21A221AC73D52AD2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3J7</accession>
    <name>A0A5B0N3J7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3J7"/>
    <dbReference type="OrthoDB" id="2507468at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022698">
        <property type="entry name" value="OrsD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12013">
        <property type="entry name" value="OrsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="445" mass="50344" checksum="538DE121B8C9F3CC" modified="2019-11-13" version="1">MSVHPTEEVPVLQLCGKDDCQLSFASANELKKHQRLVHQTHVTCALYGSVQTLVAIPRGPDGTFACPTENCTETSNDPVRIQRHVKNCDPAALAVKRDMIAPPDSKCTLVPIQDQVIGMSLLSVACKLEANQRVVAYCPVIPEYSKWLGYNRYAEILICLKCHTGVPPNEVESHMAHTHKEKIVEADIRRDYLTHGILLPDTSPKYPPRTSHLEACDPVQGLLMYLGYLCTVKTNGNICGYTTYKEKTIRNHFYEVHKGKRFISSPLFFQQDPLILTLQLCSFSDADGKWSKNCQRNYVQHLTKPVISYFCVNYRPPSPSIVEGHQAKEVIQGQDDRPTYWIKFGKVLAKMKQGVQGEGEAEPHPSLISIYLQISGVYQHIQLCKKLFPTPSVNLYNLIHFEDEAARWLPHVTAWMSSRMEGLKDLHYGLRKEATMESTCQPLCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4C7</accession>
    <name>A0A5B0N4C7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009525</name>
        <name evidence="1" type="ORF">PGTUg99_033040</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4C7"/>
    <dbReference type="OrthoDB" id="2496426at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="679" mass="79260" checksum="DF444B6CABB4A157" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6X6</accession>
    <name>A0A5B0N6X6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6X6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7812" checksum="2D8A24B8958D325A" modified="2019-11-13" version="1">MGHIFPNQRHYQKNFEVRQVKGIIILVILSFLPSFISPFFSLSVSNSNTSALSPLSFLLIYIRVLVSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9G2</accession>
    <name>A0A5B0N9G2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000108">
        <property type="protein sequence ID" value="KAA1085054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2507451at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="954"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1135"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="995"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1138"/>
            <end position="1156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1085054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1156" mass="130732" checksum="733197EDA69201B2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
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        <name evidence="10" type="primary">CHS3_18</name>
        <name evidence="10" type="ORF">PGT21_034035</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cell septum"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF16">
        <property type="entry name" value="CHITIN SYNTHASE 3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="385" mass="43700" checksum="07019174E7077F08" modified="2019-11-13" version="1">MMAYPTKPTGLAMKTLLRQHLVACCHPRVQHLSISSLVLSVLPSWSEMQVVVWWWWEHGRLQCQSTTGCPFPLYNVVPPPPDYEPFNFPLVHTICLVTAYSESVEGLRTTLDSLATTDYPNSHKFILVIADGKVKGSGNDLTTPEICLSMMKDLIVRPDEVEPKSYVAIADGHKRHNMAEVYAGFYSYDNDTVEVTKQQRVPMILISKVGNADEQHESKPGNRGKRDSQVLLMSFLQKVMFDERMTEFDYEFFNSIWRVTGVSPDHYEICLMVDADTKACMVRDSEIMGLCGETKIANKSDSWVTMIQVFEYYISHHLQKSFESMFGGVTCLPGCFSMYRIKAPKGGEGYWVPILANPDIVEHYSENVVDTLHAKNLLLLGEDRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEB4</accession>
    <name>A0A5B0NEB4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="205993at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
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        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
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    </dbReference>
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        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
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            <begin position="8"/>
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        </location>
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        <location>
            <begin position="73"/>
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        <location>
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        </location>
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        <location>
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        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="776"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="803" mass="91214" checksum="F47052BFDFA30D42" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NF41</accession>
    <name>A0A5B0NF41_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_030537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NF41"/>
    <dbReference type="OrthoDB" id="10290882at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="482" mass="55178" checksum="AFFF7E8468EAE900" modified="2019-11-13" version="1">MSDYGPSPSETIDQIIQGLNSSIERWDYKDHLAPIRRPPLYADDATWEHDNIRALRREAMSTDEVDDRKALLLQLQTCFLPSLKQQLADLLKSLDLADLSKDPKPKLLDILETVFRLGQTFKQINAFLYSMAGIAACSSQAHKKYDHDYGGVKEFRCHSLIEKINHLMSEHIWVLFKRHIPYILLRSKDHPTRLNDRSDLIAHGRKLIDGIARSSNYIDTIIKSSKRSDFNILQDKWRGKSNDLNCLLKRIAVRINPTTEEERLLIDAGLPEIPSTSKAIADDNHSTNTDQAEIDSNYQDTASDTDSSIDQEDDESDTSCSTCSSEESFLRSLLIKLAQSAIPLIKLVRILFNKLSNPISQVPFTIGLKMSSMDIESVQGELSSLNCSIHNILNDLLYMYDCEGIMDELKSVKMLRERLWRYFDSSLVLLCFYLVPSTGIELELPISGNHSKTWFLELREQFYLATKVFDKALYEFEQEIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHN0</accession>
    <name>A0A5B0NHN0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Stc1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032259</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHN0"/>
    <dbReference type="OrthoDB" id="2496880at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024630">
        <property type="entry name" value="Stc1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12898">
        <property type="entry name" value="Stc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Stc1" evidence="2">
        <location>
            <begin position="67"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="171" mass="19795" checksum="7D424D30F42D9B81" modified="2019-11-13" version="1">MPADRSNQNKGRKSIFCTACKMVKDLDQFDGHQLTKRFGIKQCAEEEEKKIPIGYDAEEEKSRAIERRALLKKIWCRRCMPQQTNEFYCSDCEQRLPRAKFAQAQLSKHKSDEDRLCKDCASLTMQKVEAVRVGEEYEADEIENKLKERRTATLSKTSNNPESDDHPTESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIH6</accession>
    <name>A0A5B0NIH6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005110</name>
        <name evidence="2" type="ORF">PGTUg99_023562</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIH6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137460" evidence="1">
        <location>
            <begin position="20"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="138" mass="15116" checksum="83BB00991EFE64E4" modified="2019-11-13" version="1">MRSVVAAAISLALFQSASASAPYALDKRGTHPGGFIPGVPNPSCETYPPPPSCIPHPRFDDIVACYYNPNPRYCRQYIPNVNYDYCSMYPYIPDCQPSFPDIFYYGASPYGQFGSPTGNAMFKAKEADGHKAKTEAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NK97</accession>
    <name>A0A5B0NK97_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NK97"/>
    <dbReference type="OrthoDB" id="10345793at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022920652" evidence="1">
        <location>
            <begin position="18"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="384" mass="44889" checksum="B5573FE57BF68183" modified="2019-11-13" version="1">MPFWYLVCLILWVQVTTIVHSKDDWEQFAEELAETIDSASPRSGDQSYEEVNLCSKKPDTDFKYNSRIPAQDSEFQAPKRPRNDRYVFSEKLGKDFHPVTSHLAFDTGFWKHIQINSPGDLDRAFEELLVLDHQNGNLSIPEGIDLPNWELNASLSETQKLRYGHIGSKYQRYYKRAIERVCKFDPPNSVDLIISLNSERTEYSSISLLEILRLLHVWMAQIHSVVGRDFYGDACCQLDHQQMTLWLFREVFNPPSGFPVLGSIERETLDNYKSEPGPVQVWLSKLLQDPRLVFTVSLAIVGIWFKNTSIKWKQHFKIDNYFWARVPLALTKHIWESENSDQNMSKYREDPISGKSKDYLGEFFIVWQPSEGKRQYFDFNPQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKM5</accession>
    <name>A0A5B0NKM5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_017283</name>
        <name evidence="4" type="ORF">PGTUg99_016900</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKM5"/>
    <dbReference type="OrthoDB" id="10323898at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137489" description="Hydrophobin" evidence="2">
        <location>
            <begin position="21"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1089811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="11761" checksum="E48CF94D1FA0B562" modified="2019-11-13" version="1">MNLSNFFNSLLVVILIQGEALHVQSFDCQKEINFSGFDQAYCATSAKGKPPKGRGQEHDPPVGPDGYNIAKPIVSNNGGNRCPSGHPTCCSSVRLKIGVKSNDLYSAIGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKQ7</accession>
    <name>A0A5B0NKQ7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RGR1_1</name>
        <name evidence="12" type="ORF">PGT21_013956</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKQ7"/>
    <dbReference type="OrthoDB" id="205099at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055122">
        <property type="entry name" value="Med14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013947">
        <property type="entry name" value="Mediator_Med14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809">
        <property type="entry name" value="MEDIATOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08638">
        <property type="entry name" value="Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit MED14 N-terminal" evidence="11">
        <location>
            <begin position="101"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="30"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="967"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="968"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="987"/>
            <end position="998"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1089323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1087" mass="124240" checksum="85217ACB8B793A24" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NL10</accession>
    <name>A0A5B0NL10_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_012686</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NL10"/>
    <dbReference type="OrthoDB" id="2677917at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="203"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="518"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="562"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1089282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="664" mass="72917" checksum="BF46388921766670" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLR3</accession>
    <name>A0A5B0NLR3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">sterol esterase</fullName>
            <ecNumber evidence="6">3.1.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_002759</name>
        <name evidence="12" type="ORF">PGTUg99_037209</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a sterol ester + H2O = a sterol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10100"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15889"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:35915"/>
            <dbReference type="EC" id="3.1.1.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.13" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLR3"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000108">
        <property type="entry name" value="Lipid particle protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="10">
        <location>
            <begin position="138"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1070191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1090211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="613" mass="69701" checksum="325398A13E977E13" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NM83</accession>
    <name>A0A5B0NM83_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NM83"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="594"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="699" mass="76578" checksum="46E99358A21E5E91" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQP5</accession>
    <name>A0A5B0NQP5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hsp70 nucleotide exchange factor fes1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FES1_3</name>
        <name evidence="5" type="synonym">FES1_2</name>
        <name evidence="5" type="ORF">PGT21_003732</name>
        <name evidence="6" type="ORF">PGTUg99_036998</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FES1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQP5"/>
    <dbReference type="OrthoDB" id="10250458at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013918">
        <property type="entry name" value="Nucleotide_exch_fac_Fes1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF18">
        <property type="entry name" value="HSP70-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08609">
        <property type="entry name" value="Fes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nucleotide exchange factor Fes1" evidence="4">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1070212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1090188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="360" mass="39651" checksum="7BBCF9BEDA996852" modified="2019-11-13" version="1">MDPKLNDLLHWAVENTNRPQAENQTRAASTAVAPVPSSDPIPLSITYNPHESSQVQAPTAPSKKLDTGVLDAILGRTDAVRMREAMGIFEDPNKPIQERCNAGEELEDLIQDLDNANDMEVLGFWPKLITLLESNPSGSDENDLIKFHTCWICGTAVQNNPKSQIAFLKRDPLPTILEILCHASEATQAKAMYCLSSTLKHAPEETQVMKKFSEAHGWEALHDCLRGPSMTLRRKTVFLINTLVLEESLDLEELRSAGLLNTLIASLSPSRAIPAGKDGELSSQDEDYVEKVLRTIATLLINSSARPNQVISDDEKNLVKEVLKELKLDSASFKQLVESSGIGESEWSQALESLGPFSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSG7</accession>
    <name>A0A5B0NSG7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_036385</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001010">
        <property type="entry name" value="ABC multidrug transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="640"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="674"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="782"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1198"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1226"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1267"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1306"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="164"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="859"/>
            <end position="1097"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1091632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1462" mass="162597" checksum="30CE4B0A5FAD9563" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTI4</accession>
    <name>A0A5B0NTI4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_034481</name>
        <name evidence="12" type="ORF">PGTUg99_013587</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000379">
        <property type="protein sequence ID" value="KAA1092012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTI4"/>
    <dbReference type="OrthoDB" id="2510127at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="59"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="378"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="379"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1081350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1092012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="418" mass="47229" checksum="631013A2D9C34B9F" modified="2019-11-13" version="1">MDHSLYGAHSRIQLFLADLHCVLGSLPPPTLHGPMQAIFNSIGSQHNLNRLNVSNQRISWKRPTHRSRSWRKNRTGTDCQRSPQVHHPLPIPPVKQNTGDTPKEYEIRFPQHQQLAHIHNHHKRSRPQKSSPVLGHIQSNDQHFDFQYSYNREPQSTEEYHAYGNSSLEPPINDFGNCLDQAQDEDLDSVSVKSRDESVDLDEVEWDDRSVLDINADPDHLEQQPEPAFDLKSYSADDVDRWASTLSAEEFEHLRVMGTDARTESFRQYAITNLDQPTQTLTPQLNQEPPDHIPGEVADNHDQDANNPCVHSHHVNHQPNQYSCSSYYEHSNYSTSNLDQNPDNTAGFDGRFDDGGFENGEFDDGGFYNGGFDDRFDDGGFDDGGFDDSGFDDGGFDDGGFDDGGFDDSGFDGGSNYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZY8</accession>
    <name>A0A5B0NZY8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoplasmic reticulum junction formation protein lunapark</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_031099</name>
        <name evidence="4" type="ORF">PGTUg99_013495</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in determining ER morphology.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The C4-type zinc finger motif is necessary both for its ER three-way tubular junction localization and formation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lunapark family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000444">
        <property type="protein sequence ID" value="KAA1079075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZY8"/>
    <dbReference type="OrthoDB" id="1725934at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098826">
        <property type="term" value="C:endoplasmic reticulum tubular network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071788">
        <property type="term" value="P:endoplasmic reticulum tubular network maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903373">
        <property type="term" value="P:positive regulation of endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040115">
        <property type="entry name" value="Lnp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019273">
        <property type="entry name" value="Lunapark_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166:SF12">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10058">
        <property type="entry name" value="Zn_ribbon_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Lunapark zinc ribbon" evidence="3">
        <location>
            <begin position="261"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="317"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="373"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="377"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="452"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1079075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1094835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="467" mass="52422" checksum="DCAB8BE85E932088" modified="2019-11-13" version="1">MGIFSFLRSSAQPTPDLFEKELVELELKIQKHQDRLKAIDQRQRSTTSTITIYSSLFWAIYSSLWYLGWTPIKWTNLNSKLLSPDSKTLLLLSTNPKFNQALQVLPIILIPIGLISLRWLVQTWYQRKQESEKLRLRLLQKQLRDKVEELKKKTAYYSTKELLDRYDEKTKLNPQSSPSNPHQLGRMTMTPPNNEALRHRQRPPSSSPANLSSTLSPIQNNHPSPAYQTGPPSTGATSSAAHQQLDQKMSNSPSPSIGRGWADRFAEVLLGDDEARPESKYALICINCFAHNGLVRQEELDHIQYLCPKCGTFNPSKSKGIHQKHPSGTEAQTENSQGKRAGRKSLADSMIRRKPPIDDLFNVTDSRRVVSSVAGVPSTSTTSLSLDQASPHPAGSNGTVMDDEEDQRSDQEPDRPDSSLAKLPRDNEGDQVLELDRPITPPDIPSESSKDRKLKKKSSGQKAKQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P518</accession>
    <name>A0A5B0P518_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P518"/>
    <dbReference type="OrthoDB" id="19657at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050471">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433:SF5">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="62"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="327" mass="36409" checksum="7C23081EA404548A" modified="2019-11-13" version="1">MLCNVAADLPTAAYRFGQRELRGKRTLPPIMPTKSSHQPRHIYYEVHGDLKASQKIVLVMVQYFSKRKDHVVLVFDNRGTGNSNAGAIEAYRTSDMAQDTVTLLNWLGWNQDRSLHLFGMSLGGMIAQELVRESSRGLACLIVPKRFKSATFISTRCGKRRLGGFSEDICEEGERRPGVGSVSQRFVSKGIYGNCSRARHATQERLARKLRNCHRLPREQAPIHSWDTFNAATHASLPSPEIAQDRADLSPPKMLILTGGSDELIPTKRSLELHKNLPGSELVVLENAGHALIFQLSEEVNTIMERAIVEGNQAFKTCTVVDLFGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5Y1</accession>
    <name>A0A5B0P5Y1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030781</name>
        <name evidence="4" type="ORF">PGTUg99_019186</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5Y1"/>
    <dbReference type="OrthoDB" id="2498862at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137675" evidence="2">
        <location>
            <begin position="25"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1107976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="702" mass="79192" checksum="7CC359CFC77BF7E9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P788</accession>
    <name>A0A5B0P788_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001751</name>
        <name evidence="4" type="ORF">PGTUg99_035749</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P788"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033846211" evidence="2">
        <location>
            <begin position="20"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1134368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="193" mass="21322" checksum="C04901F233D1E4C4" modified="2019-11-13" version="1">MPFQKSLLLALLTASLALGRTVVNLDHGTGAILHERSVVPLLKDPSYRPLKTDDGMTQSKFRNSVPENEHPKKKKKRSPQNANLAKEEGNAISDQRKIRVYNGGKGHPNFRKMNHANPLLGPGHHHIYHLDSTSSGFEIAPIPHNGRKTPLVIRSENSPAPFPGTFAAATRDEELYLPDSARNTHGDHHHHGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9N6</accession>
    <name>A0A5B0P9N6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002379</name>
        <name evidence="3" type="ORF">PGTUg99_035472</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9N6"/>
    <dbReference type="OrthoDB" id="2506076at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="478" mass="51785" checksum="CB16997DBAF69063" modified="2019-11-13" version="1">MDHSSPLHAGHRQTHHIIRSARHLALRQLVSRPEIQLGPIDTSHTNTNTGATPIPVKPVTNVITAGETHTSPSLAAPAPLPVTPPTNTVSVLPHLVTPTPVVRIANVPQATATSAIPSKTSLPLSPCSAGVPCASMIAKSAAVVSPVPQQTQGTPQFFASMSSSPGAIFVTCLVGIAIIGVMLSILSCALRSWCNRRRRSNIDEDAWRFLENPKDFSDPTHEEEDVHSAKKSYFGTDSQSSLAHLQPTLAKNYVASHPFTSHIQQQTQQATSQYHYTGFPAQSASAVNGFIDEQPMSGYIDEKGVCWVAPPAPVSSQCNGILMTPCGPPQERMESYQGGCTIPELVFRKTSLGGNFCNRATMYASPTQAPVPMLTRPASVITRTSTISSNQHNRLHSLTREASLACSVQSTTVEELRERVTEQRTDLRATLRGAIQKDKKADNDLLESLILLWHDKNTEAQKEPIRLQTPQLPPKDHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAH2</accession>
    <name>A0A5B0PAH2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012679</name>
        <name evidence="2" type="ORF">PGTUg99_017917</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAH2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1134026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="50" mass="5619" checksum="3C565A0E0B425477" modified="2019-11-13" version="1">MIIVRGGTVKARVQSFFVSRGYRLICDSGRLHPPQLDVRRSVQQASMTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBW9</accession>
    <name>A0A5B0PBW9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021847</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBW9"/>
    <dbReference type="OrthoDB" id="3264316at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272">
        <property type="entry name" value="BED FINGER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="142"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="250" mass="27967" checksum="A63B3BD009651CF1" modified="2019-11-13" version="1">MLKKMIEKTETYLAEALACNTILLTTMLNPSYRLSIFQAYFSSHHKYAEILLQQKFDEHKAELAATVSSRESTPQQPSQPQKTSHRRPIDDLNLFPGAVESENTDELANYLGGKYKVLPADADQCLVWWKVSILFGISEYCMSVKGSFLFCISLQEHHNEFPTLASLAKDYLACSATSASVERCFSAAADICGRDRGSLAPRTIEKSVSSHQWLKQGFKANGDFERAQVIVSQDLVDHHESRHEAATASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCH4</accession>
    <name>A0A5B0PCH4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription elongation factor spt6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SPT6_3</name>
        <name evidence="2" type="ORF">PGT21_002265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCH4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="176" mass="19642" checksum="014B0AA310AAB4B4" modified="2019-11-13" version="1" fragment="single">MKTKEDAESEMRIAPHEKARNANIVIDTSIQRRKRHRSVANDDDVRDQWTWMKDDLELLAANTGVPTQRKAKRPIATRGIGGSDNIDQDVEDDLIGFSTMKMTDSKTSARPQDEFPEDEPSNLPSRPSKRKDKHVRSSQGTTGRQKTGGSLDGALAPDVSDITKESWDEVIEVLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCL9</accession>
    <name>A0A5B0PCL9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020326</name>
        <name evidence="3" type="ORF">PGTUg99_002257</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000343">
        <property type="protein sequence ID" value="KAA1099051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCL9"/>
    <dbReference type="OrthoDB" id="2507355at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="461" mass="53208" checksum="E3151159CE8D3E29" modified="2019-11-13" version="1">MESVAEIVKSFKSINPDGKSEQEYQETIREVIRHFKARVRGHASRKNFDHRIKYVAPERLGQNILPALKRRLWLFSLALNVSNSPKDTKSWYSGILDHLADIDDLLEDIDTYIISIWRSYTPNQGYPVFVDDIPFRVERIAEHVRELFESQLGRLFDAYHTFFDDFRLLKPCSNALSSPEEGNTVYRLTAASSEKIDDILESVDKSSLGLAKEGWQKLVKEIDTSFEYPLEGPVLNPLDPDYPNGYEELILGIETRRNKSFQAATPVVKLCRIYFKKLVRPTNNQPLIFVAPSMNMTEDQLMRFVEHTNRTDSFMYNFVSAIIHWSDQLDTVFGVTVELQLHFRELSPVLEEYWESLLESNNLRVDQQAIANARQWLESWKSHFSLAMENFIQITGCNDFPRPIIDERHTGVEEVSDESDTEQSDQENGSVFEYGSDGEDRLDGDGSLDGEEDPHDEDNTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEZ7</accession>
    <name>A0A5B0PEZ7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_017909</name>
        <name evidence="2" type="ORF">PGTUg99_026855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEZ7"/>
    <dbReference type="OrthoDB" id="2518223at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22813" checksum="D04B0D19DA8FCD58" modified="2019-11-13" version="1">MPLFEGDGGKRRKRIRKPKTKAGVSFSQAVQLEHQHILQTPLAQPANDLHPTDHPNEDSSAQENNNQTLDPTGQDDFDFGADHIGNNGLDPFESAHQQDRLPSDHQDNDHLTNSLVQQLHRYFQSEHYKQKKILEEKNWEEAYQEMFSSFQQCAIKTFEWGNPNLWNHDWNEPCNCNNLRSRPVVLVDMYSKFSSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIB2</accession>
    <name>A0A5B0PIB2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan 1,4-alpha-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,4-alpha-D-glucan glucohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glucan 1,4-alpha-glucosidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SGA1_2</name>
        <name evidence="12" type="ORF">PGT21_018315</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal (1-&gt;4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIB2"/>
    <dbReference type="OrthoDB" id="6123450at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004339">
        <property type="term" value="F:glucan 1,4-alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000084">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000165">
        <property type="entry name" value="Glucoamylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF9">
        <property type="entry name" value="GLUCOAMYLASE, INTRACELLULAR SPORULATION-SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00736">
        <property type="entry name" value="GLHYDRLASE15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022683580" description="glucan 1,4-alpha-glucosidase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="GH15-like" evidence="11">
        <location>
            <begin position="64"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1101387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="463" mass="51790" checksum="06E4E1ACD82D2D32" modified="2019-11-13" version="1">MLSIRTLLAHLLVLDVCFALTAGGPPSTPPTYPYKQQVQHPDLDAWIAKQADLSWSNALTNISPHGALPGLIVASLSTSKPDYYYSWTRDTSIVLKEIVWRYQESQDPKLLALIKQTISATQVIQRSSSGATAGLGEPKYFVNGSAFTGPWGRPQRDGPALRASVYIKFALAYLRVGGPEAARYVKSVLYDGQWKSRSVIKADLEYISNSWKERSFDLWEEVNGHHFFTFAVIHRALKDGASFATKMEDRGAAAWYQKQADLIAVQLDSFWDTQARYIQASRNFVDSHGKKSGLDVGTLLGCLHAGTQAPQSFRPDSDKMINTFHAILQSMSKTYPLNSRFHDLTAKAVGRYPEDIYDGVSTSVGNPWFITTLTMAEYIYRLNPKTDLRTNRWSGSDGETQNLTSRMIGDQFVSIVRNFIQPNGSIHEQFDRVNGAGIGARDLTWSHISFLSMDRARRGLPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PII1</accession>
    <name>A0A5B0PII1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_035382</name>
        <name evidence="10" type="ORF">PGTUg99_004459</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PII1"/>
    <dbReference type="OrthoDB" id="8783038at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20824">
        <property type="entry name" value="C1_SpBZZ1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11912">
        <property type="entry name" value="SH3_Bzz1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000296">
        <property type="entry name" value="Actin polymerization protein Bzz1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000157">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035459">
        <property type="entry name" value="Bzz1_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="F-BAR" evidence="9">
        <location>
            <begin position="7"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="8">
        <location>
            <begin position="408"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="544"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="627"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="484"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="484"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1100268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1102035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="687" mass="75656" checksum="FD098219B77B1743" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKI2</accession>
    <name>A0A5B0PKI2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Galactose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="4 15">2.7.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">GAL7_2</name>
        <name evidence="18" type="ORF">PGT21_035737</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="4 15"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKI2"/>
    <dbReference type="OrthoDB" id="418412at2759"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00608">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000002">
        <property type="entry name" value="Galactose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019779">
        <property type="entry name" value="GalP_UDPtransf1_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943:SF1">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00117">
        <property type="entry name" value="GAL_P_UDP_TRANSF_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10 15" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="8 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase N-terminal" evidence="16">
        <location>
            <begin position="19"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase C-terminal" evidence="17">
        <location>
            <begin position="232"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="12">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="44"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="93"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="182"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="348"/>
            <end position="349"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="353"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1102075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="382" mass="43748" checksum="038269AD56FD4503" modified="2019-11-13" version="1">MTSWAWPTTSEYHTHTMQTFDPTQHPHRRFNPLTHQWVICSPHRNNRPWQGQIEPNEDTRLPSYDPTCYLCPNNTRASGQVTEKYTSTYFFENDFAAVNDVEVSQKSDSDPLRMALHRTEPARGKCYVICYNPRHDLTMAELTPDEILPIIKAWIELYSKIKREQPFIKYIQIFENKGAMMGCSNPHPHGQVWALSYIPTIPTTVLKSQEDFASSAIDVPDSARLQDGRPSLLLSYAHSELHPEPSARVLHRTEFFLAVVPFWATWPFEVLVLPHSHHMASLLDLPPGADLDLAMILSHVTRAYNNLFRCSFPYSMAIFQAPPHSEASVAQLHISFCPPLLRSATIRKFLVGFEMFGEAQRDLTPELAAEKLRAAALQPYYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKK2</accession>
    <name>A0A5B0PKK2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009571</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKK2"/>
    <dbReference type="OMA" id="MRIMADY"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022973147" description="SGNH hydrolase-type esterase domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="36974" checksum="BB74798CD715D09B" modified="2019-11-13" version="1">MLLQANTPMLSVFVILCLTFISSRCDDQSNQRGEIPRSANQIKMAVQPQCGTLSSDKFVNFNTGINLDQIKTIYSFGDSWTSNGNYKGGVPQPAVPKGTEPHYGGRACNGPMWTEDLASGGKTLKSYAVGGATIDRTVWKARATKTDMVGHVDTFLSQKITVEPESSLATIFYGLNDYSASLQGPGSFEEAVKQLFNQTERLISAGLTQFIVVSPPYTHEIVKQFDSSIWNGFKKLKESKNIEFAYVDLVALFDAIHADLNSFGYKSDQACLKSPDTTVGGCENPNEYLYWIPSHPQYQTQQLMADWVRTVLEKCHQPNSNRRKRANSSKLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKQ0</accession>
    <name>A0A5B0PKQ0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_023716</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKQ0"/>
    <dbReference type="OrthoDB" id="2505871at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="8"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1101533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="74" mass="8331" checksum="50755850D91D4B4C" modified="2019-11-13" version="1">MALHTNASQFFDPGQYLIGDAAYNLTMTTIPPYKVPAANLLENVEFNYCLAKSRVRNKHAIGVLKARWSSLKEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMD8</accession>
    <name>A0A5B0PMD8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PMD8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="200" mass="22427" checksum="77F46002DA33EE6C" modified="2019-11-13" version="1">MSDQESDEGMFKAMIQANAQLGGGGDSGEQLCRRQLHNPLVIALNMENRRCWIGHRLFRKSWDHVGCGICNLSLHASKSQLDTPRCIFGSFFDHRPPFETIFRQSTLFFPLPDPGENHQSDTLKSVRLMVFLLSIGHSQSSVRWTPGCTPSSGWSRLPSIPWTLSEASKRCPGRPSVRWMLRNQVAGARDGIHPFAGGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PR31</accession>
    <name>A0A5B0PR31_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PR31"/>
    <dbReference type="OrthoDB" id="2495363at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="492" mass="57146" checksum="EE1B320A6F9C3BF7" modified="2019-11-13" version="1">MEFYIQRLYLIFLLTGQAFILTMLISTHGEETNFKTVDLNQLPPDEPPSSPFCSLTPDEHPLSFQLRSLPPTPPLDASVEDALIQRYLGLHSASSAPKSFLTNHRPTGPYKRKSLDTNVEQPKSNLIDSEQKRKKSLLRYQQTWPAENESASSGDTADAIRLRQLEKQPIESFKNAPTDQYEKGLQLEESERKIVLFNVKYWSFLIHKSNTEIAKDQQNTSEQFVPESLFEFLNELKTEIIVKKGGKRPANPPGNPDKHLWISKEHATDFLKTYRGRRDMMPYPLTFTSPERCLQTYNMILMLADEKLRLEDYPVFSEGIVAWINLKLTRKLANIPGKVTKRKKQITARVGIVEKLTKCITFFNIIYLSLLNEHQNGQLTRGYVDDFLSFLRTLWHNIERGEEDQLFRETTFAWKLHDMLNFGKYWGPTKSSTNAMGIAWDVLQYWANQKGIFFPKSPNNLSELVNKIIVYSNYRTINDKIRLINWQLHYTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PS45</accession>
    <name>A0A5B0PS45_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PS45"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="385" mass="43323" checksum="21F506A8F61B30C4" modified="2019-11-13" version="1">MTPPTWPMSSGLQGQDFHGPRTQGFMAQGGRPRTPRPTKPRKLYRYRFSAKNWPLEPINPKRSRRHYRSPQPPRSPSTHPSPKETTQAITIPHRPVSSKWTKRNQQKRLIDRRSLKVATQGVILTQSYPTSFDRLTNGQLNPAIDQSLLTTAIHREFFIIFISMFDWVNVDLSAVPSLIGCFLFSAIEVGSHSTQPSNVSSRPPPQLTAEPLTEGQETTAIYRSDNSPIDGLFVSAIKPVAIQVQRIIDRLLTSVIDLLVLKANRRYWHSRDRPSAPEGYISDSAIHRPISKIIDRSSTPPSECSVLTPCCPESDRSSQGQCLPHPHKFSDQPWVHSLIDCLFVTAIKPAVTQPLFKVRLSYLLTPIFKGPISTPPRITDGNLHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUB0</accession>
    <name>A0A5B0PUB0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_015320</name>
        <name evidence="7" type="ORF">PGTUg99_036389</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUB0"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044695">
        <property type="term" value="C:Dsc E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF162">
        <property type="entry name" value="TRANSMEMBRANE E3 UBIQUITIN-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="303"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1068703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1104224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="381" mass="42234" checksum="317DE59452250B67" modified="2019-11-13" version="1">MDYIPDPNALDSPVEFSAGPFTPPGTPPPLPGVSFTPPGTPPSLADGSSTPQGPPPPLAAGSFTPPGTPPPLPEVSFTPPGTPPPPPLPPLPPSHFVPIPNPVVFPDDDEPMEGLPAWWPLPPPLPEVSFTPPGTPPPPPPPLPPLPPSHFVPIPNPVVFPDDDEPMEGLPVEEAWRPITDLTKNCQPQIGAVFQTLAPQDQAKMTRFLRKMMEVFLTLFQNRQRDRLDARENSYLLRDLPQIRLILQASEESDAQLLTALIDHWISLIHAFSNARRSQRVQDLLDQMKNPPARSARRAPSECPICMEILSNIRHSNVRAPCHLTHVFHRNCLQEWLEGELNCPMCRAPFALPRRRRQQTLVRLGRHRRAFDLLVLLCWGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXU0</accession>
    <name>A0A5B0PXU0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_017483</name>
        <name evidence="2" type="ORF">PGTUg99_005515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000385">
        <property type="protein sequence ID" value="KAA1090411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXU0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="72" mass="8182" checksum="D864C254196FEEBD" modified="2019-11-13" version="1">MVGIPAGTFTKLGFLVLFFSFLRQPGRNSGYFLGVSNYLYSRRTCYNVLGNNFDRNHYANSVYGRISGPNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PY27</accession>
    <name>A0A5B0PY27_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021171</name>
        <name evidence="4" type="ORF">PGTUg99_006422</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000041">
        <property type="protein sequence ID" value="KAA1135089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PY27"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137849" evidence="2">
        <location>
            <begin position="23"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="138" mass="15449" checksum="AD5B0CBDF760AD92" modified="2019-11-13" version="1">MDLCATALRVTLVLVLVSIAHAQRHARCPNAQTCGGKGIPLSEEERNQMSLKLRDLCGWEYQDGHKCEKECDKTYYSCMTCCKIFSKNTAWDDRSKLGCEHSAKRFVDVPAPSGTEPSPSEPGPSEPDNRPPFLYHFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q203</accession>
    <name>A0A5B0Q203_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disintegrin and metalloproteinase domain-containing protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_002103</name>
        <name evidence="9" type="ORF">PGTUg99_006233</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable zinc protease.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000147">
        <property type="protein sequence ID" value="KAA1127892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q203"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000003">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF159">
        <property type="entry name" value="ADAM METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13688">
        <property type="entry name" value="Reprolysin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137879" description="Disintegrin and metalloproteinase domain-containing protein B" evidence="5">
        <location>
            <begin position="25"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="7">
        <location>
            <begin position="315"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="6">
        <location>
            <begin position="568"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="484"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="483"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="487"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00056552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00074021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1107099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1127892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="759" mass="82234" checksum="E99C653F4E7E0D76" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q3A4</accession>
    <name>A0A5B0Q3A4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020242</name>
        <name evidence="3" type="ORF">PGTUg99_018430</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q3A4"/>
    <dbReference type="OrthoDB" id="2509728at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="487" mass="54881" checksum="B2E6FC80F875FC79" modified="2019-11-13" version="1">MSNRLSTEDILSTIPPGVNPFTYLLNLITPQFLVPFSRFTTAILIIFFIFHLLIAIFCLVLLVLPYLRGIKRSQWLFKRLYIQNHSGENVYETPLLFVNTGILMAISQFIGSIAAQAFIFIQIKTSHSSDYALKSPLASPMGVMFMCEVLIYWSLSHCFLVTIYYDRKTHGEITQGTRTWISPPAVINFVFLSFPIFVVAATIALFTWLSSGFTHFMVAAVKVLDALRQGSSAWDQLKVPSTSSEEKLHLTTQLIQVVSEAKNFTTDVNIRSKNLINCFHLVQCVLLIIICTTSLVFIVSFWVLVHKFRRRHRQSDKASSRSIYLRRWGKRNNSRNASGEQSTTSSNLNPSFIDVVKSDRQFLHLALRAISIIIAMLTTISLLLLGIIRTADVVKYPYWRGLSTWLTTVSGTWAAIPIAWQCWRLYQEQSGGASQSLSNSNDTALIGVPGSKNADPGRPTQDGAETIEIKIELDPKAIHPTETFEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q3L8</accession>
    <name>A0A5B0Q3L8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020992</name>
        <name evidence="4" type="ORF">PGTUg99_014567</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q3L8"/>
    <dbReference type="OrthoDB" id="2507694at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="2">
        <location>
            <begin position="173"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1124551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="351" mass="39355" checksum="B0DA8DDE6EEE891F" modified="2019-11-13" version="1">MTTINQLISFYNNNNQNQQQIPDPSSSNTRPATKTSVASDHHSKEPLLLRLPPKKKIGSEECYQIDLQQWLSLGQPQPTTPPKQEQIKVPACTRRSVSRSPPPVPPRKTHTHSTQTIPADSQPSQKEKEEEEGPPIDLGCFRKAIQALWTTLIHQHFHHPLHSSSSASSYYDLNNQKITLPGSLIRAVWIRSGLSLQTLAHIWESIDQQKTGGLNFEQFLLGTYQISKLRQAQALKTRKPAQTAHPENRRRRTSESKGGEKEEEGGSEMRRIRRMPTSGGRRTPPLVPSSRPASSRSASVCSSSNDEASLLDHPIALNHPVIHFHHLHHPADLFIPFQISPSPFLKSHPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7C2</accession>
    <name>A0A5B0Q7C2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_029944</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q7C2"/>
    <dbReference type="OrthoDB" id="7722975at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000043">
        <property type="entry name" value="Ser/Thr protein phosphatase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.780.10:FF:000028">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006146">
        <property type="entry name" value="5'-Nucleotdase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053828">
        <property type="entry name" value="Nucleosidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014485">
        <property type="entry name" value="Pesterase_C1039"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF22">
        <property type="entry name" value="ADL392WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21953">
        <property type="entry name" value="NadN_nucleosid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017316">
        <property type="entry name" value="Pesterase_C1039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00785">
        <property type="entry name" value="5_NUCLEOTIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022836746" evidence="1">
        <location>
            <begin position="36"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="66"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Putative 5'-nucleotidase C-terminal" evidence="3">
        <location>
            <begin position="408"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1108969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="673" mass="75504" checksum="CD3EAEC4844876C0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7R6</accession>
    <name>A0A5B0Q7R6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029554</name>
        <name evidence="2" type="ORF">PGTUg99_013837</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q7R6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1106134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="97" mass="10681" checksum="9180F718C725FE69" modified="2019-11-13" version="1">MSPKVTHIVVIRSSFNIVNRITAGLGFLDRRLLSALALLTSTAVNQFTPEVRTIGYGFFLLKPALNDGATSKLVNSGANRFSDRTHRFQHSTDQAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBB4</accession>
    <name>A0A5B0QBB4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WHEP-TRS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_023867</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBB4"/>
    <dbReference type="OrthoDB" id="5844513at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000738">
        <property type="entry name" value="WHEP-TRS_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00991">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WHEP-TRS" evidence="7">
        <location>
            <begin position="107"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1110518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="204" mass="21864" checksum="2F981B2E2643BB89" modified="2019-11-13" version="1">MPSTSELILKQLNAPKRRLSRTFGIDLLPGHTIGDAIYLFRKIDPDRAAFWRKQYGGSKPSGDPAVVEPSGESKKAKKKAAHGGKSKGTTPAGPLFSGPKPPEMVEIELAIVAQGERVRQLKAGSMEGEVGMEVSKLLGLKKDLALLIEQLSTEGFPSAGRGTCTSSKRKAFLPMDEVRVPRPAEGNPSAGLYTARREGFLPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC47</accession>
    <name>A0A5B0QC47_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032534</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QC47"/>
    <dbReference type="OrthoDB" id="17948at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="93" mass="9875" checksum="D10ED5A441020BB1" modified="2019-11-13" version="1">MPSPLLTVSKGVLFEAIYSPTTSLNSLHLHLSCGLPVSASSSTCRRNLISVLKEIIRVTNCAHGLVVSSGAARWAKLRALRDVINLSVSTSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC50</accession>
    <name>A0A5B0QC50_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chitin deacetylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QC50"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12415" checksum="72454FD7EA584220" modified="2019-11-13" version="1">MQAGLAEYPVGTIFNSDPNGICSYRRASCLRGETKDRGIGLQDIWLGKNHTWSINFDDGPLPPSTSLYKFLDQQDETATHFWDQMSVIIQSWCYRLGNEVTTLLHGCTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCH5</accession>
    <name>A0A5B0QCH5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinetochore protein Spc24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_029770</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Centromere</location>
            <location evidence="10">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SPC24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCH5"/>
    <dbReference type="OMA" id="NMENEPA"/>
    <dbReference type="OrthoDB" id="2500316at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013252">
        <property type="entry name" value="Ndc80_Spc24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08286">
        <property type="entry name" value="Spc24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 10" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="88"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1110694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="251" mass="28649" checksum="BB199B416304B1C7" modified="2019-11-13" version="1">MSQLRSSWIPSSQPESHSQMRGTNTIENEAGERSMDQHQTAADEPPSQSDIWMTTLVLAQDMAVVLKEGVEEEVGMMYEALDQTFKWNERRRERVENAHNELLRVNRQVEAKKDALEKMKANSTARENQAEIYSLDQSAYEEARKIKGLDYSIGSVKARTTKLQTELDELEQSEPTRSPDFVIDSSVLKTKLYHDFGFVQVRTPLQEGNNSDPEKLFIRCDSDCTASRVVQKPKTNRDGFTLANEIWDAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCS1</accession>
    <name>A0A5B0QCS1_PUCGR</name>
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        <recommendedName>
            <fullName evidence="4">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCS1"/>
    <dbReference type="OrthoDB" id="286814at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF36">
        <property type="entry name" value="PHO85 CYCLIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclin"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1111017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1059" mass="116897" checksum="134FDE1ACD0EBB15" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0QDK2_PUCGR</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="663" mass="75777" checksum="019BB87D8CD2AEA3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHK2</accession>
    <name>A0A5B0QHK2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    <dbReference type="OrthoDB" id="8041940at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="188" mass="21021" checksum="E91A17296CFCC1DA" modified="2019-11-13" version="1">MTALTNVAKRSEKNKKSFDKKLGTIAPRYAIGESVKLRNESHVKGAPRWYGPFEIKRVLENNVYILSDTEGSDYLRPVNGNSLRPVSLRSLLTNEMWATPPIIALKERQKDARVAKKALRNTKSIAKTSKPGIRLKFEGKFITPKTGTASKIRGGGCCSRRHDDTAYLGPRRYTLDPHVSFFPLFGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMM2</accession>
    <name>A0A5B0QMM2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SAGA-associated factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_008934</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SGF11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMM2"/>
    <dbReference type="OrthoDB" id="21557at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071819">
        <property type="term" value="C:DUBm complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013246">
        <property type="entry name" value="SAGA_su_Sgf11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051078">
        <property type="entry name" value="SGF11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46367">
        <property type="entry name" value="ATAXIN-7-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46367:SF1">
        <property type="entry name" value="ATAXIN-7-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08209">
        <property type="entry name" value="Sgf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="144"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="219"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU261113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1114439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="363" mass="39495" checksum="AF2CAA7231E3AEFC" modified="2019-11-13" version="1">MNNIHNSPHNQQQLPSDQSSSTGETRPYFNQRAPPDVGNQAPISQDDGIGMLVDSVFNGLLEEMIWKICVLEFKRAKKFHLFSAEQELYANEKKNGNEKSNPDPVHDCPVCGREVSAARFAPHLSKCLGIGGRGQAAKKGIRSDFSESESVTKGSSHTSLDFAAVSRNRTGGNMKRRGRPPKKLPAVPLSPAEHEALLSNAINNATQRLHLQGIGMAVTSSHKPAPSVPHPLSQSHVATPPVTKVKRKKKKDRNRSRNSSQASSSSDSEEEDGADGEEDGEEQEGDDGEDDHQPFTPGGRIDDENDTGGQQRGISENPSISSTSVAPVAPATTAVLPRKFFIMPTRLPLQPRIHSRQSHFISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN03</accession>
    <name>A0A5B0QN03_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN03"/>
    <dbReference type="OrthoDB" id="2511106at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="496" mass="54754" checksum="BF5443AEDA24BF63" modified="2019-11-13" version="1">MYQSCHRCDRTRSAIIVPSPRYSSFLSVGTSLLRLVRSVTISLSLEFNRSPLNLDSNLVTRPTATTSRLCPRNSTDGGSISTLRMSLDLNTVTSPIWLQSCHRANFLLTENNGLSLLTGKPSAPAFCALCHLAPFLPTPPRSNDTPKICRTTLVAMPSCYSQAPWVRISPGGISAPAILSGIKGILSSSSSFPPHQLSTTASYIHFPSKLFHYPSTIHSPFYCPTMSSIFDNCDTNDFYCEGRSDPSIDIGVRYYPDRDQWSPIFNPRVVPDDNADGNKGPIEAVGNKGPIEAVGNQGPVDIKPIVEAKEKGPAEATVDPASINTIDKWHAINDPSRHYDCVPIPRLFPKPHPFVCVPWSVHDPELPPGLPVPFLRNLNLPVPAPTPAPSTSSSESLSNDSDSSSTYPLISNYINLNHQGVQKLLLDDNTPPTAVCIYTEEDVMRIFEGIAPEYVDMCSEYPPSSLAESDRRVMFFENMQWFFRNKRSTYLSTRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQK3</accession>
    <name>A0A5B0QQK3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQK3"/>
    <dbReference type="OrthoDB" id="2511178at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="507" mass="57192" checksum="1674F04F5C34DCB7" modified="2019-11-13" version="1">MDFSAKLTPKDSKFAFFRIFKVFRLTLNQNHQQTNQPRYRLALNLSKGFQLPVLPPRGRARTLTTRKNIIPDSPKDTSPLPSSPLSTIPSDDLIENQARLLPREGNFVGSTAWTPISTTSEQFRKYKKKKSLVDTFSCSKSATENLGKSAKKPKNRVNSASSSLKRTTNPITNPSTVTEKEKNKKKSSSKSKTSSSQGQIQQPISPNTTVNKTSNSTQQDTSIPRMAETSHMSSNSDIASAQQPRTGFESLESSLPPINEDTLLRPQAQWPAQDQGVSPALINSRAASIALESNRSTPVPHPIEREQMPFLNNYTDRPPQPRPFMTTSLANQMEDIRRTTTPSQAPSPFIQTRPQEQEHTEIVVDNADLDMWLNLFNNQFFMFARAQETSNVRDMRFILTQAAETQDRILEIVGREETIRLSENWHPREELALLEESLTRTTDRSGVDKRTPTPMAHANLHAIAEWRAGRHATRLHGVYPAGWRACLHAQYLVLNAYLYAFRTWAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXM7</accession>
    <name>A0A5B0QXM7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXM7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="205" mass="24505" checksum="DC796D1CEAF69D2E" modified="2019-11-13" version="1">MLPHDRIVIHQHKIEHKQFDLVLTKYLNYISLINAEKLIKLIGKGNQLRRNSLIVNRLYYFMMIKDEKEKRSIFLHVGKELKILKEKMIPEEWNVLWEIYESTRKKIKPSAYSIWKEQVILKITKLPFHSAKILDDILFDCSKEYLLTLYEGESLEETYGKINEELLSEDAKRKLEFISELWKIQDIDRNIVLNSPENLLSIKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0X6</accession>
    <name>A0A5B0R0X6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0X6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="22859" checksum="E697E3A7CCE2B31E" modified="2019-11-13" version="1">MHRRFPQPAYVASTPRAFRISAASIPHAPNKPAKALVQARSRHHIPPTLKILSPGSSSPPTSIPVSHLPTMSTCSNCQSRNTTCVRESSNHACDFCRKAKHRCSLVQHPSDRSRRRPRANSPDSHGRPRPPKRPRTTTRGNSLDDFVVPDSASVSVASPTPSSRPPSPPPQPPQSSPESPTGSDRMTPRPHESDFSSRQKYRAAVVKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1C7</accession>
    <name>A0A5B0R1C7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Importin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">IPO4_1</name>
        <name evidence="8" type="ORF">PGT21_018964</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1C7"/>
    <dbReference type="OrthoDB" id="10535388at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="HEAT" evidence="6">
        <location>
            <begin position="112"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="345"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="389"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="472"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="497"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1119250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="597" mass="65688" checksum="48F1BFAFB1CF6499" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1C8</accession>
    <name>A0A5B0R1C8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1C8"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37471:SF1">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37471">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="67162" checksum="BDE662497716E577" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1M0</accession>
    <name>A0A5B0R1M0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026457</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1M0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="70" mass="7583" checksum="44CEE512C29ABF36" modified="2019-11-13" version="1">MACSTIRLTLTDPLAQQYRKVKPVLSSKVVAADDLLSIEELSTDCQIADRLVGGLDPSWSAVRDSVGNYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3N8</accession>
    <name>A0A5B0R3N8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030178</name>
        <name evidence="2" type="ORF">PGTUg99_035047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3N8"/>
    <dbReference type="OrthoDB" id="2497957at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="241" mass="27690" checksum="EC5E074A6B1B2A7D" modified="2019-11-13" version="1">MLNATDFRRFKSDVQQHLQLEAQRKQQHEDHVEEIDSTFHHLNQHPSATLLSNRNLKGSANELRVACALGSRTSNRLLESSCQIMEAFHDSLLRRTIAINLEYELKRQLFPTLADSSPQRSQSSFMSVREAKKEDPERAKRILKGWFTSRQDEHQFWAAMHILKSVSSSVHPTNTIKGDPMNVSTTCTLLEQDFLPATTLRDQGRSLKWDRKKKQLIRRLVTKLGQASPSDGNTTFLHSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R463</accession>
    <name>A0A5B0R463_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_037300</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R463"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="52"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="262"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="740"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="654"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="792"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1120240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="878" mass="98173" checksum="C2300EC136E885F5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R8C0</accession>
    <name>A0A5B0R8C0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028981</name>
        <name evidence="3" type="ORF">PGTUg99_032434</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R8C0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="112" mass="12752" checksum="B9CA0356B6F9D4F3" modified="2019-11-13" version="1">MGGIKWTSSLESLTGPPRLGMPEETAQDQTSQWTSTAKSDWTFTKRKINSKFPSIWQSDQSSSDPREPTSIEQHNTTGSHALISECWVRENDGEEQRYRNPARYIFSCHSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RFU6</accession>
    <name>A0A5B0RFU6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_018579</name>
        <name evidence="7" type="ORF">PGTUg99_024732</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RFU6"/>
    <dbReference type="OrthoDB" id="129353at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17745">
        <property type="entry name" value="BRCT_p53bp1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17724">
        <property type="entry name" value="BRCT_p53bp1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047249">
        <property type="entry name" value="BRCT_p53bp1-like_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047250">
        <property type="entry name" value="BRCT_p53bp1-like_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047252">
        <property type="entry name" value="TP53BP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321:SF3">
        <property type="entry name" value="TP53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321">
        <property type="entry name" value="TUMOR SUPPRESSOR P53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="908"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
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            <end position="273"/>
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        <location>
            <begin position="293"/>
            <end position="317"/>
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    </feature>
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            <end position="342"/>
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        <location>
            <begin position="648"/>
            <end position="659"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1107576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1124630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1235" mass="132970" checksum="E07B0557005BB0DE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RID7</accession>
    <name>A0A5B0RID7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014839</name>
        <name evidence="3" type="ORF">PGTUg99_032857</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <dbReference type="EMBL" id="KAA1107467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124725.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13796" checksum="5C07BED0C000C970" modified="2019-11-13" version="1">MPGLRTIARSAFVCALLLKPFLVAVGIPGAPYDPDLYTDRCQQCWVAAGSRPAFINTMQKLSTLPSFPCSAFSHVYTCFETVGLQGYYCGRCHHYVNVSLRACPNHGQFTQYIPDPQWTEQGGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RLX8</accession>
    <name>A0A5B0RLX8_PUCGR</name>
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            <fullName evidence="3">Uncharacterized protein</fullName>
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    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
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    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
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    <sequence length="400" mass="45004" checksum="0D0D03572BF61086" modified="2019-11-13" version="1">MAATRSSRTPKTHKNSKKTRSTATLTVANQNKSKTKANSSARKHASRTRNNGTSDSDRHSDPDDAREEEDPRPEGQNSPSDFVGLTVANFESQLPKWSIYQLRQALHKSKDPPANRIPPEVQDALTLLQQNYIKSKLMLAAIGNVSEETVNKSLGENKPSRRKCAWNRFLAFSLESANTPVPPKGVSIGWEERNQHLGEVWSSLTEDEKDVFSPKVFQYFSKIPCRFGEEEDDNEDEAEPELTSEEIELYQPLYTKLVNQEKVDMIVSKGENVGIAKGTAFKLAERSMQRLNSELFTMSNSYNTTYYLLTATRAPGKNSFCKEWSNDAAWLTLAEKNWSAREKFEAYSHGREIQESVEEANGVCMKKMRPADAMKIKLRAALNETLGELATLAISLVQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S0P6</accession>
    <name>A0A5B0S0P6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007069</name>
        <name evidence="4" type="ORF">PGTUg99_012750</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000107">
        <property type="protein sequence ID" value="KAA1130623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S0P6"/>
    <dbReference type="OrthoDB" id="2496071at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366655" evidence="2">
        <location>
            <begin position="21"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="456"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="267"/>
        </location>
    </feature>
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        <location>
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            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1130623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="659" mass="74878" checksum="3EB59AAA0217825E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKQ4</accession>
    <name>A0A5B0LKQ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKQ4"/>
    <dbReference type="OrthoDB" id="2591449at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="396" mass="44086" checksum="33B68B322C9401BD" modified="2019-11-13" version="1">MSTETPAISTPPSSHQPTDLPSPKKLEMDDQGPSPMTMLTFFKGPSRNKLAENPVRKSICFGLVSHHPSQQPQPNRHADRPRVVTNWKSESETQREILNSLEQPVFSPTMSHNRSSSSHSHPTDQRIEPLNLKSIPAEVLLPFNERENEIVELLAEPQNLGLYKRLKDRLLQAESWPKFEKGLLRASREEIGDREWIVAIRALIEPLDDHLWVSFRGLIGADGLDDQESKPAKEEDWHVQPFKVRRSSIRSTDGRRRMDSHHSRSISSDSSLSGVGTLESDMEDSFSMVSQSPRARMDSIKEHDFLESELDDPEAPGCDSPGMIPQTGSDSGDDQADDSHVLALKILDGPSDPIPSSSDPKGDVKHAYCLLPHGLKYSFGQVVDHGLGLQSLNVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLQ2</accession>
    <name>A0A5B0LLQ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLQ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022800398" evidence="1">
        <location>
            <begin position="23"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="130" mass="14670" checksum="1A5FFA98045F8649" modified="2019-11-13" version="1">MKLASLSFAISVLIATAPECSGLKTFQLGDKTITYLNVHTEETPQFSIELGSTFIKLNSYTQRPDTSPCGQPARYVMMCSSHGNITMDSNRTLKMLMRADGINPKMFLVQPWNLYDKPENYKYTDLLAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LN53</accession>
    <name>A0A5B0LN53_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LN53"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="143" mass="16077" checksum="CC5496972A775B5C" modified="2019-11-13" version="1">MRLTPSSKIANQERTSVVSSGPISRTNRARWKRSVKSLPSQEQYVVGLSRGQELKHKTISHPPVKHPSNIRPTSHQENQMGRPKEIASKIISDGTAIPLDPSNQRIIRASFFYGPPHCLMAAIFPQTNYNRRTLVSQRLVQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQM5</accession>
    <name>A0A5B0LQM5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_024751</name>
        <name evidence="9" type="ORF">PGT21_026683</name>
        <name evidence="10" type="ORF">PGTUg99_003753</name>
        <name evidence="7" type="ORF">PGTUg99_024308</name>
    </gene>
    <organism evidence="7 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 12">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
        <text evidence="3">The crystal structure with reduced Cu(1+) has also been determined.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000319">
        <property type="protein sequence ID" value="KAA1103687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQM5"/>
    <dbReference type="OrthoDB" id="1921208at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00920">
        <property type="entry name" value="Cupredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002387">
        <property type="entry name" value="Plastocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052953">
        <property type="entry name" value="Ser-rich/MCO-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883:SF15">
        <property type="entry name" value="EXTRACELLULAR SERINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883">
        <property type="entry name" value="SERINE-RICH PROTEIN, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00157">
        <property type="entry name" value="PLASTOCYANIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0186">Copper</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033473428" description="Blue (type 1) copper domain-containing protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="6">
        <location>
            <begin position="52"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="166"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602387-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1066782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1071986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1078010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1103687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="297" mass="31158" checksum="7BD7EFF6FC32AC8E" modified="2019-11-13" version="1">MSKLFHFLLVFVTWASIVSPLSSQGPPPPPPEKKTNPGQPSNIPQGKNFVIKVGNEEAAKIFSPATIQAQVGDMVTFEFHPKAHTVAQASFDKPCEVLASPEGNGFNSGKQTVELKTPNSNLPRWTMQITKPETIWFYCDPHCGLGMVGAINPPTSGEKTFEKYVENSKKAGSKPKNLRAGSAAAPGDPALRLGENPGDKNAGRLADKAGGQTTDLAGAKPGGQSGTLQRGPPKTANEVATLNANSASVDGKDPSTLASKGKDGGSGAASYFMEKRRAIISSVSLSMILPGVLTTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRT7</accession>
    <name>A0A5B0LRT7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000471</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000191">
        <property type="protein sequence ID" value="KAA1066849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRT7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="90" mass="9887" checksum="A7D59B9FE7809155" modified="2019-11-13" version="1">MGRAHGCIRGASRLKGRRILTDHPGDNRPLYACPYLQCCIPGGDRSANCLDYHVRTGPPPSARSLGNSINQVIVARLSIAEGWVFPEEWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSG2</accession>
    <name>A0A5B0LSG2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TATA-binding protein interacting (TIP20) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_009422</name>
        <name evidence="7" type="ORF">PGTUg99_031062</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CAND family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSG2"/>
    <dbReference type="OrthoDB" id="6260732at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010265">
        <property type="term" value="P:SCF complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039852">
        <property type="entry name" value="CAND1/CAND2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013932">
        <property type="entry name" value="TATA-bd_TIP120"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12696">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08623">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25782">
        <property type="entry name" value="TPR_CAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="56"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="TATA-binding protein interacting (TIP20)" evidence="6">
        <location>
            <begin position="1089"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="321"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1067285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1071486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1273" mass="139943" checksum="8E5E90E81D456050" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXH0</accession>
    <name>A0A5B0LXH0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">chitin deacetylase</fullName>
            <ecNumber evidence="9">3.5.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_014561</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + n H2O = chitosan + n acetate</text>
            <dbReference type="Rhea" id="RHEA:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9597"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57704"/>
            <dbReference type="EC" id="3.5.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:10465"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.41" evidence="9"/>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXH0"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004099">
        <property type="term" value="F:chitin deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050248">
        <property type="entry name" value="Polysacc_deacetylase_ArnD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587:SF135">
        <property type="entry name" value="CHITIN DEACETYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="11">
        <location>
            <begin position="50"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1069155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="165" mass="18545" checksum="CCF8A250727D1DC9" modified="2019-11-13" version="1">MQTGLAEYPEGTIFNSDPNGISSYRRASCFRGETEDRGISLQDIWLGKNHTSSINFDDGPLPPSTSLYKFPDQQDKTATHFWVGSNVRDDPELVLQAWKRGDHLAVHTWLHAHLTTLSNFEILGELGWTIQIIHDLTGLVPLYYCPPYGDVDNRVRALAKFCPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2H5</accession>
    <name>A0A5B0M2H5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015464</name>
        <name evidence="3" type="ORF">PGTUg99_024701</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2H5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366072" evidence="1">
        <location>
            <begin position="16"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="82" mass="8563" checksum="47AC6340D1D423FA" modified="2019-11-13" version="1">MKITMLFAALSAASGAFTAPAQAAAAAKDLSIGAGVGIGIGAGVGSWGYPYGAYPGWGGYNYYPYGGYPYSGYPYGYRYYQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M4N5</accession>
    <name>A0A5B0M4N5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M4N5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="476" mass="52774" checksum="D82FF58144A2AE3E" modified="2019-11-13" version="1">MSSNPNNNLLSTASQMKRNNANLAKENAESEETHNLGNPSLNPIPSTLTQSIPPSTNPNPTPIPQKSNPPTAKAPIGSNSQQATSKSAIPPGASQKHPLSLDEVATNVERQRQPAKQVKDLAAILGTKTSKQCNHTEKAHMERTIATNATLIREAEKEREKDEQTATILEKALKADAEGDKTRADMFYNIYAKLVTGKETDPAPRDREDQHHQTDAGIGKKNTVAGGTNFNWGDANSHDDVGFTPYFDKNILELKGPLPLTIFNKSWQDAALAYHAEKKHKTDNNSTEKGLRYTGLPYPSEWHVYKYETLGEWIILHKANADKILRKDGFMVALRYDIRLRANAFAHRVVKNGVKSFSDISIFRQEVYDTAYAEARKYDELVFREVNPYASGGVRASWDPHTGSKKPTVQPATSGHQPRNSVPPAQSSSQNTNPLPARPRENRRGSGYQGRNFNPNYSGNQAQGSNRYQNGEANKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M4S6</accession>
    <name>A0A5B0M4S6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cell wall alpha-1,3-glucan synthase ags1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">AGS1_11</name>
        <name evidence="1" type="ORF">PGT21_029029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M4S6"/>
    <dbReference type="OrthoDB" id="512920at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047657">
        <property type="term" value="F:alpha-1,3-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070600">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058655">
        <property type="entry name" value="Mok11-14/Ags1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47182:SF2">
        <property type="entry name" value="CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47182">
        <property type="entry name" value="CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="116" mass="12873" checksum="5D85754FB0CB1B98" modified="2019-11-13" version="1">MKEKSHEFRAGKGEKSEMGMISAGIGAAMGLRTRGARWPDDMTVRRKVLIATLEYEITDWKLKVKIGGLGVMSSLMGKAMHDVDLLWVVPKVKDLEYPEAEPAPPIEVTIFGEKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M537</accession>
    <name>A0A5B0M537_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Importin-alpha export receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CSE1_1</name>
        <name evidence="10" type="synonym">CSE1_3</name>
        <name evidence="10" type="ORF">PGT21_003477</name>
        <name evidence="9" type="ORF">PGTUg99_004953</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPO2/CSE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000482">
        <property type="protein sequence ID" value="KAA1070924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M537"/>
    <dbReference type="OrthoDB" id="2501327at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000057">
        <property type="entry name" value="Exportin-2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005043">
        <property type="entry name" value="XPO2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF8">
        <property type="entry name" value="EXPORTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03378">
        <property type="entry name" value="CAS_CSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="29"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1070924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1099320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="995" mass="112320" checksum="D53488E06FFA0E8D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M887</accession>
    <name>A0A5B0M887_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034055</name>
        <name evidence="2" type="ORF">PGTUg99_012585</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1072975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M887"/>
    <dbReference type="OrthoDB" id="2509601at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35711:SF1">
        <property type="entry name" value="ECTODERMAL, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35711">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="426" mass="48871" checksum="D84879C8F9ADCC34" modified="2019-11-13" version="1">MRTFEAKTLSQLRDLQRTHIHYKKLNLAIKIEAQDLYFDYQRKQHLLSLKHSRPFNLLTKYLGQRRTRQKETSWHKFRKNNPDAKAALKNTNNNIGQRNKDVGKLYKQHKATQADPEPSNSQVTVDSDADPNAEERGRFGKIFKSNETLRQEVKDWANGVQLKLKELSDSFGVEGFLVLAAQDHRKPLFFQGGSLLGDDYLRGLIDEGNPMRKFALWTAGSKAASKKRKAPDDSNATANKDTGEASNKKTKYAVAAFENRDVCQGYLALNHKYIAEELGLMFKAAQSKSTIKGKLRPWPGTDTVLRLARINHKLVIQKNPVGLSVEHLINVPVKKMSIEHTWLVLRGLKNKWINLVEHQFDTLPKSAHVRKSKVTTANTTASRQEEEEYDDEDDEDDEDDEDDEDDEDDEDDEDDEDDKDEDEDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8P8</accession>
    <name>A0A5B0M8P8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_035582</name>
        <name evidence="10" type="ORF">PGTUg99_026926</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1073092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8P8"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="9">
        <location>
            <begin position="284"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1073092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1084804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="456" mass="51759" checksum="4631B635B0F715AD" modified="2019-11-13" version="1">MASSSTTAELFATNEFERMLPGYLFLIPLLTQTFLMNPCYENSRSAKLLRAALMPITIYLTFSRVAIRLFYPLNVFFHWNFTFVSFPTFHAVCLAIQYGLSQAPVFASKEEMIKAGNYRGDPDEKDEKEKSEIQCNPNPSFLEKIKFTIWLLFSPRGLQTSWAPSLEVVPRGPKMGAGLFFVYVLFKTVICHFLMTLLWMIGVECAQHPHGAYGVVADWVPSLGFLRDYPQFDFLTPAPLGGAAWFAIDTLGGILTLLEVILYQVGPYVLPKDLAPGKFDSTLYPPLFNDLHARESLIAFWSKGWHAIFRRNIVFCGWNPMEYLFSSFGKDAAKTAGMMGGMIFSGIFHEYLIAAVSKIDWKFPTVLMFTLCGLGMVTEVQFKRRTGLLVKGIPGRWWLVLVIGAGGKYMVDSWMARGLGRSDMPPPRLWTWPRFLVPFSGLMPERWISRIESWVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MD61</accession>
    <name>A0A5B0MD61_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007200</name>
        <name evidence="2" type="ORF">PGTUg99_027723</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MD61"/>
    <dbReference type="OrthoDB" id="21617at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="232" mass="26595" checksum="F898FEE371464C76" modified="2019-11-13" version="1">MANIKLEHLQELLQSLQKQQQQTHTNSTSTSIENESNPHKRQKLINQPSTSSTSTLKHHHQAQQDHPNQQPTTQPILNFDQALTTLQKTIELQPGFLDKIQQIKEDQDQFELRSIDQRNQIQAELDRKLKKLINSSSPDNSSSSSSTTTTTKIQNEARTENLKIEYESKLDLFDRSVLSKWDQLKLSQSITLQNLNVPFFSGESDQSDPKMITQQNLVINFLMNSINECPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEL2</accession>
    <name>A0A5B0MEL2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="8062037at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="312" mass="34341" checksum="1330FDE624BA15BB" modified="2019-11-13" version="1">MTPNSTSSDSESAADQPGPSPGGVLPVEESVEPQASALEPQLHVEHDEAPATPQRHLPPHAEHFGEGNWIQLPEELNGTIRALLANLQGPTEIELDEEGAPRLPDAQDEPAVTQNALALIRHFEAMPAIVADEPHQIDANPWQQMLQTLTPEQRRVVENFENRTITVRRELANNSVQSNDPMAVLQGYFFHLEELRQTLASLPHETANMIEQFNSNAFQEIGRLMVNSDRIDVAALHIGYLLDSLPITSLSSLASDVPLHDSMKCTICLEEYASSDEIVGWLQSSFPGPPTCPNCRALILFQNHTALPPQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MER0</accession>
    <name>A0A5B0MER0_PUCGR</name>
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        <recommendedName>
            <fullName evidence="3">PWI domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">PGT21_012997</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
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            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MER0"/>
    <dbReference type="OrthoDB" id="163257at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052225">
        <property type="entry name" value="Ser/Arg_repetitive_matrix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23148">
        <property type="entry name" value="SERINE/ARGININE REGULATED NUCLEAR MATRIX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51025">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="560"/>
        </location>
    </feature>
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        <location>
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            <end position="189"/>
        </location>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="328"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
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        </location>
    </feature>
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            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="547"/>
            <end position="560"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1074598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="582" mass="66361" checksum="5ADCF87434087543" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MF18</accession>
    <name>A0A5B0MF18_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH CENPB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_016379</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MF18"/>
    <dbReference type="OrthoDB" id="2507562at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00674">
        <property type="entry name" value="CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="3">
        <location>
            <begin position="295"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="616"/>
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    </feature>
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        <location>
            <begin position="73"/>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
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        </location>
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            <begin position="381"/>
            <end position="395"/>
        </location>
    </feature>
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        <location>
            <begin position="422"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="470"/>
        </location>
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        <location>
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        </location>
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        <location>
            <begin position="520"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1074706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="677" mass="74878" checksum="C262794D3D2B1322" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHJ5</accession>
    <name>A0A5B0MHJ5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">HRR25_10</name>
        <name evidence="4" type="synonym">HRR25_3</name>
        <name evidence="4" type="ORF">PGT21_034895</name>
        <name evidence="3" type="ORF">PGTUg99_036196</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHJ5"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001123">
        <property type="entry name" value="CK1/CK1/CK1-D protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1091501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="258" mass="30092" checksum="80AF3BF47A8F9C2C" modified="2019-11-13" version="1">MVIELLGPSLEDLFNFCKRKFSLKTTLLLADQLLYRLEYVHLRNFIHRDIKSDNFLMGISKCANQVNIVDFGLAKAYCNHKSLIHIPYREDKNLTGTARYASINNHQGVKQSCRDDMESLAYMLIYFLRGSLPWQGIRGETKMQKYDRIMEKKMTTPIEFLGRGLPREFAIFLKYTRCLRFDDKPDYDYLRSLFRSLFVQEGYSYNYVFDWSLKTHNGNASCVPTREQIAAQEPTVPDPPVAGLALSLQRMKIIDYHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MI46</accession>
    <name>A0A5B0MI46_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000835</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000151">
        <property type="protein sequence ID" value="KAA1075734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MI46"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="62" mass="6662" checksum="CA0717F96B22A3A3" modified="2019-11-13" version="1">MAIEASTVGCQLEVIHPRHRCAAGVLSKDNVPALGSAQTLTGEVLSPSARPLEWDMFQSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIU3</accession>
    <name>A0A5B0MIU3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_003294</name>
        <name evidence="6" type="ORF">PGTUg99_002402</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. TRA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000459">
        <property type="protein sequence ID" value="KAA1077097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5570127at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05163">
        <property type="entry name" value="PIKK_TRRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046807">
        <property type="entry name" value="Tra1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046805">
        <property type="entry name" value="Tra1_ring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF1">
        <property type="entry name" value="TRANSFORMATION_TRANSCRIPTION DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20175">
        <property type="entry name" value="Tra1_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20206">
        <property type="entry name" value="Tra1_ring"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAT" evidence="4">
        <location>
            <begin position="2572"/>
            <end position="3141"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="3">
        <location>
            <begin position="3384"/>
            <end position="3707"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="5">
        <location>
            <begin position="3715"/>
            <end position="3747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="559"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2010"/>
            <end position="2047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3151"/>
            <end position="3213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="571"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="586"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2016"/>
            <end position="2032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="3161"/>
            <end position="3197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="3200"/>
            <end position="3212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1077097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1117316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3747" mass="425197" checksum="1BE0C0BF3D4950F3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MK69</accession>
    <name>A0A5B0MK69_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Histone-lysine N-methyltransferase set9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SET9_2</name>
        <name evidence="2" type="ORF">PGT21_005679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MK69"/>
    <dbReference type="OrthoDB" id="6627536at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042799">
        <property type="term" value="F:histone H4K20 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039977">
        <property type="entry name" value="Suv4-20/Set9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12977:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE KMT5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12977">
        <property type="entry name" value="SUPPRESSOR OF VARIEGATION 4-20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="426" mass="45461" checksum="74E1748ADE04ACCD" modified="2019-11-13" version="1">MPSLGTDTDLSSLGDSSEGTTSRASNTPALETLPPSLLLHNALPYQLKSSAAAFVSPVKFRPTQDLTLSILRPPEPERVEPQQNLITKIEPPPVSEPVKQPAGPKADGQVLPVKRKTYWITTKQKQLGLVPWEADTITPTSSTSQPYSPMTPSLLGPSRRDSMDRSVSGQKRKAPTESCSARLPKRSYWVSTKEKELGIVPWEAGAADESPEPSAAHPRSCRSTRASRRASEGMDAVIGFPTAPRGSKLSYKVFQEGTQEAEMLNTGHRQGAARLDSADTPLDLNGDASIGPYDEIASFLLRYPGEQQPTGAQSGGESGGLYDGLPIHLHILHLAQAPLPPATRGGQGASAQTCQSTQEKPHSFPAALDTPSEHSGSLSPVPTPRLIPSHRSNDNIPRDIQPKDDPASSAKSPSTNGHRLLVSSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLX2</accession>
    <name>A0A5B0MLX2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022992</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLX2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="235" mass="26457" checksum="49AEFCF89D10EB9C" modified="2019-11-13" version="1">MLTAIYEELPPRGEKMTAAKICTQVERNWAMRIRMSYARLVMVHHYVHKYRKTSQWLEIDERLGILRGSLVDFQRHHTQLVLDKDNELFSHLKRFDKINKEDFTVPSLEDVRKSIAATALNNEATAATLNNNQAVNGFALANQPTLDVDASPALRICRRMALRKPAGGRYADGWQSVGLAWENTPTTGLDGKLALEDKPTAASDASCYKTSRWAPKFFGGVREVGSTKFFTLALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMD2</accession>
    <name>A0A5B0MMD2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_019285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMD2"/>
    <dbReference type="OrthoDB" id="2555434at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38646:SF1">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38646">
        <property type="entry name" value="YALI0F00814P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22648" checksum="174A6006E2D3114F" modified="2019-11-13" version="1">MISSIDQTHQPCGSSSPTSPISVSAQEASMPVRNRSDPSDEDGVPDRCESEPATQQRLSCANRRHLYRGHRSGSLIVEDLDELTEWRARQRTFDGAYLRSALGCSFFALTITKMFRKELSQIGLEFAALSLLILSISYIRRRRLNHDFADAHFPQPTELALNEAQGPRVWGREYRTPGDFVLLMAAAVAAIQIGLIILLANMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMZ6</accession>
    <name>A0A5B0MMZ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMZ6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="93" mass="10551" checksum="859E5745A91C26CD" modified="2019-11-13" version="1">MIGGKSMRYRRSALSEARRVVWNSGRKRKSGCDLGASRNHLDRSMPTGMRPVIPKRSMWFMPVRNSADSNERTYANILNFVNTSEGIFIPSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSQ6</accession>
    <name>A0A5B0MSQ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytoplasmic tRNA 2-thiolation protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_004279</name>
        <name evidence="5" type="ORF">PGTUg99_020594</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1080005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSQ6"/>
    <dbReference type="OrthoDB" id="25129at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019407">
        <property type="entry name" value="CTU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882:SF14">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10288">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="332"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1065591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1080005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="48314" checksum="DA626800AE7C4A90" modified="2019-11-13" version="1">MRNYLNTNQDRSTAKWKSPIDFSTIEVISIDFGSASSYISIDSSSETKLVNEEGNRPPTENEGTLRSMVESLGFSLKILNLDDLFDSPASVEESSPNGCSPGCFVDLSDPNLPIKTIAPTDPGFSTPSSTETVMKPLANTLRPLTQTSRRSLIESLINKRLLDFCKDKPRNKVLVKPTTSTQMAIDTLAGVSLGNGWSLDQQIGPSSYIHDVLVCRPLAQIVDAELFQYSKLMQLDHPYPLIHQAPTKKADIPALITDFVVKLEENYPMTSSVINQTVNKIGKNKSALASSSTTSTKTTRTATNGKDCSVTRTCPICHLSSDREANEWKRSITLSTHEPPTTATPNASSDESEIVRPVEEEEEDEPMRLRDQLCYACLVTFKDHQYIAHSSQSGHDWVQLPGFYQPLTQLPVAPAATPPRPSLSANHLQAVLDQFLISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUJ6</accession>
    <name>A0A5B0MUJ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUJ6"/>
    <dbReference type="OrthoDB" id="2498123at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="310" mass="35025" checksum="38C22456F8E6D12B" modified="2019-11-13" version="1">MALLNPVRYVPPKSTRSRTESYFNLNTESLDSRGPSELRPAVAPTGTDQIEPVKLERLRPTILKNQAQIDAMKADNKHIISLIKDLKKRDDFLGGKVEDFYKHLKILGQARLYDLCIDSPLTLGTRHSTIQAPSPSLTEASPCEVARTPSAPNLIVSLVKCLGVILPFNKNEPHFSKSSDEIITESSQTLQPLGSRNTHQGGSPKMETASKESELHGEPLRMIQSMVFPLDELWMGLESLMPVGLPKCFGEWKTSDRPLIREWYYAVTWLDYYSSQERSATYSHLASTHKLKFLNQKLLCQRSYFTLPNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MY41</accession>
    <name>A0A5B0MY41_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021481</name>
        <name evidence="3" type="ORF">PGTUg99_000847</name>
        <name evidence="4" type="ORF">PGTUg99_014224</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000216">
        <property type="protein sequence ID" value="KAA1122786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000047">
        <property type="protein sequence ID" value="KAA1134682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MY41"/>
    <dbReference type="OrthoDB" id="2509538at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1134682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="123" mass="13410" checksum="29529FFC72566BE9" modified="2019-11-13" version="1">MTQLKVGSNRAARWASAVGSLGAGPARSDSGLRSEFRLSIGQKGISIDTLDERRGRGRYSFNPRPGQTSVYCQPLGSFLNNCVDCQWSRRSCYAIARLLNGLTTLLLGSSPVLLHFALSLRFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N105</accession>
    <name>A0A5B0N105_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004444</name>
        <name evidence="3" type="ORF">PGTUg99_028934</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N105"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="81" mass="9157" checksum="1A3B9CADBECEBE3A" modified="2019-11-13" version="1">MMGNNRDILAQYSTQGSPTDNLMVDTYKMGIQNMKKMICKDRDLSKFKKHVHVQHHNNDSEATPDDIENPSADEASGSEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1G2</accession>
    <name>A0A5B0N1G2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024467</name>
        <name evidence="3" type="ORF">PGTUg99_017136</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1123996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1G2"/>
    <dbReference type="OrthoDB" id="3264316at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="33"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="106" mass="11704" checksum="277A5923DAC9B84D" modified="2019-11-13" version="1">MDLFPQAIEASVPNKLAIYLGGKYKSLPGQEGQCLQWWKEHHKEIPVLVSLAKDYLACSATLASVEQCFSAAADGFKANDEFQTAQDFVSQDLMEEEESKTKFAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAU1</accession>
    <name>A0A5B0NAU1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012587</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NAU1"/>
    <dbReference type="OrthoDB" id="2509760at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="364"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="580" mass="65023" checksum="89264C98164FA708" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NB59</accession>
    <name>A0A5B0NB59_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MED6_2</name>
        <name evidence="8" type="synonym">MED6</name>
        <name evidence="11" type="synonym">MED6_4</name>
        <name evidence="10" type="ORF">PGT21_025821</name>
        <name evidence="11" type="ORF">PGTUg99_023357</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NB59"/>
    <dbReference type="OrthoDB" id="344220at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.580">
        <property type="entry name" value="Mediator complex, subunit Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007018">
        <property type="entry name" value="Mediator_Med6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038566">
        <property type="entry name" value="Mediator_Med6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13104">
        <property type="entry name" value="MED-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04934">
        <property type="entry name" value="Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="173"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1085963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1113809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="265" mass="30080" checksum="747660DBDE784846" modified="2019-11-13" version="1">MTSTIAGLPIPDSDLCHVQWRSLAWILEHGPLTESNAIDYFALSPFYDRKSTNQVLRMQSMFSGQPKMDPASEQEALRRFIGIEYALVLSRPEPDREGGLFIIEKRERKGYDEYYPIASYYILKTSIYQAPSLYATLSARVLTSLSALQRLLELARQHKPTYDPRHSYAWKIKEKPEENTVDKQSTDQPTTESTNQIEPTTQGSDRMDIDMTAPEQPDGPAKTKLKKNNNDTPELSNPILFRALQNTITNFPAFKPPPAVDAVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBQ2</accession>
    <name>A0A5B0NBQ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBQ2"/>
    <dbReference type="OrthoDB" id="10338309at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022693266" evidence="2">
        <location>
            <begin position="27"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="383" mass="41366" checksum="46EE00222A0A67C0" modified="2019-11-13" version="1">MKAIYLPLIPMHPLLLLLSFHHLLTTVRSPGLPPAEVMSSSAAIGRQARSKGLVSPLLPGSQKLPTPPINSDFRGIGQTLVRPPESGLMTFKPAEKAPFRSTQSSNIPTAVHSPEIAEANRPQAYPLQRPPGNHHKMGSPYNHNPVQNTGRQVSEKSVTTSPPQLHPPQPLAPERIAGQEIPSRNNPSRPTLPSNWNIGVKTEKTPITPAPDYPRYPLPKPAPGDAERNVRPEALLANKPNLPLPVLGSKAPGTSAHQVPMTHKLPPGVSNYVAKYSQPKDNSFLAKSNLPAGSKSEVANLSKLSTPQQIRIQQAKPPPPLSLKTSSRGPENPMSQPNKKVTHQLPVPPFEPIMTVRNPRELTWMSRPGSQPLPAPPADGPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND80</accession>
    <name>A0A5B0ND80_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND80"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="51" mass="5832" checksum="6BA741183549CE0D" modified="2019-11-13" version="1">MYFCKIAFQTKQAQLSEAKMVASTPAVHSLITSEWIDDHSNQPKRDCLPQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEU3</accession>
    <name>A0A5B0NEU3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_021434</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEU3"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="52"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="761"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="633"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="768"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1087084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="827" mass="91562" checksum="FE5D318E5A48B921" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFE2</accession>
    <name>A0A5B0NFE2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFE2"/>
    <dbReference type="OrthoDB" id="2511178at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="489"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="523" mass="58492" checksum="94B711F91D18FAA3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFG0</accession>
    <name>A0A5B0NFG0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methyltransferase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SKB1_2</name>
        <name evidence="4" type="ORF">PGT21_011876</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFG0"/>
    <dbReference type="OrthoDB" id="1368803at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
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        <property type="entry name" value="PRMT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035247">
        <property type="entry name" value="PRMT5_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738:SF0">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05185">
        <property type="entry name" value="PRMT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17285">
        <property type="entry name" value="PRMT5_TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRMT5 TIM barrel" evidence="3">
        <location>
            <begin position="10"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 arginine-N-methyltransferase" evidence="2">
        <location>
            <begin position="95"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1086790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="151" mass="16642" checksum="670743EB552EF4F7" modified="2019-11-13" version="1">MGAAIDVPALINRWTAEPIGFLLIPATSFISNAKHYPVLSKSCQTFVKAMLKYKPSVILSQTSEGKHSSGGSLAYAQYIRFLEKRAAAIVCDPIENFAAGYLDYLQAPLQPLADNLDSVVYEGFEKDPVKYSKYEEAIFRALCDRPADATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGM8</accession>
    <name>A0A5B0NGM8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGM8"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1087722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="115" mass="12185" checksum="7CF10B18AED0E6E1" modified="2019-11-13" version="1">MDGDGSRNSGLGSQDLAKGLQKLQVIGRVILGQDVTIITWDVGIFPVEVNTIKVVLLDGAQNGLDECFAAFFVGHQFAEVLTSGPATNVITGFTTSSGNKVIEMGVEVPWDTFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHP0</accession>
    <name>A0A5B0NHP0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032509</name>
        <name evidence="2" type="ORF">PGTUg99_010299</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000371">
        <property type="protein sequence ID" value="KAA1095614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHP0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1087488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="108" mass="12179" checksum="E895DDAB09D7B4E7" modified="2019-11-13" version="1">MIKRRALELKRYLIPAINLSRPMRVKKKTPEAKPLTATSQFALTSNVPRSSPGVARDVLPGAEENWMEPCKRASRFRVSPPPLIIQPRITGTPVLERHVHGFREGNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHV6</accession>
    <name>A0A5B0NHV6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein translocase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TIM44_2</name>
        <name evidence="9" type="ORF">PGT21_033469</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim44 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHV6"/>
    <dbReference type="OrthoDB" id="10265990at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039544">
        <property type="entry name" value="Tim44-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tim44-like" evidence="8">
        <location>
            <begin position="349"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="212"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="510"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="539"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1087548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="583" mass="64064" checksum="9A1546AF317FA5A2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJ35</accession>
    <name>A0A5B0NJ35_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000104">
        <property type="protein sequence ID" value="KAA1088554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJ35"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="71" mass="8075" checksum="137DF16CF86DC00B" modified="2019-11-13" version="1">MSSKLKSLSSVNPTQQDYESRKAKGPTPPRPAPPHTKTDASHRSIVPPLRYPQHFLPFRKVLELQTKVSFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKY0</accession>
    <name>A0A5B0NKY0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Biotin carboxylation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_017330</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKY0"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF33">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE SUBUNIT ALPHA, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0092">Biotin</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="5">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1089403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="412" mass="45264" checksum="6CE0231A625F96D8" modified="2019-11-13" version="1">MHGTVHIRFDWRLLLHGNEYQHPITEMITGQDLVQWQLEIASGNPIPLPQSQIPCIGHAFEARIYAENPRRNFLPDVGPLIHVKTPKESKSVRVETGAESGDEISVYYDPMIAKLVVHSPKDRTSALTLLHKKLEEYQVVGPSTNIEFLKSLSSHPAFIAGEVETGFIEKHNEQLFPPLSDRPPEETLINATICSALQEIKARQRRASAGQANNPWDTLHGWRLGGQKISRQYEFNMVPADSLSPMSPPCSVNLGFSGVGNSSRTVDFDINLAPLESEGNESSHQVKTYLDTASNSDNTITSIVNGKRDVVVHVAQGDHSGAFSKRHHVFSGDTVTSLGIPWPRWIKDSKGEESDMGLAGNAVQAVMPSKVIKVMVAPGDKVKAGDGLIVLEAMKTEASHHSAIRICSRRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NN90</accession>
    <name>A0A5B0NN90_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PPG1_1</name>
        <name evidence="10" type="synonym">PPG1_2</name>
        <name evidence="9" type="synonym">PPG1_3</name>
        <name evidence="8" type="ORF">PGT21_017256</name>
        <name evidence="9" type="ORF">PGT21_017366</name>
        <name evidence="10" type="ORF">PGTUg99_031450</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NN90"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="7">
        <location>
            <begin position="109"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="288"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1089399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1092888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1115774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="337" mass="38581" checksum="BA890094063B0621" modified="2019-11-13" version="1">MAFSLDECIEKLLTKQLLVEALIKEICEKTKELLMRESNVVHISAPVTVVGDIHGQFYDLIEIFRIGGFCPDTNYLFLGDYVDRGLFSVETISLLTCLKLRYPDRVQLIRGNHESRAVTQTYGFYAECVRKYGSPSVWTYFTDMFDFLTLSVVIEDRIFCVHGGLSPSIHCIDQIKVIDRFREIPHEGPMADLVWSDPDPDKEDFAISPRGAGYTFGAQVVKKFLTINDMHHILRAHQLCMEGFSVLYDDRLSTVWSAPNYCYRCGNMASILEIGPDGHKHFNVFDAAPENERDGPQQQMQLQQQQQQLQMQHQQNNGGQDNGMANPYMLPQVEYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNZ9</accession>
    <name>A0A5B0NNZ9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNZ9"/>
    <dbReference type="OrthoDB" id="2518589at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022949408" description="RING-type domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12161" checksum="AEF46C55A1652929" modified="2019-11-13" version="1">MSSWKKIKVAMLIASCISSAQCLLCPNKCKTLSNYMQHFYNLATCNTMTSCPVHPQCVKCKKMVPQQTHTCPTCGHEWTVAVVTECPRLQPEGDQHHNPICKIPNTAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPU1</accession>
    <name>A0A5B0NPU1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016920</name>
        <name evidence="1" type="ORF">PGTUg99_025891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPU1"/>
    <dbReference type="OrthoDB" id="2507305at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="368" mass="41511" checksum="106A3818F2BC78E2" modified="2019-11-13" version="1">MTPKLFIVGFLTSGNSDIAYQRRFWGTHTGWQSTAELLRVIKAVVLQAQDGATSWNQFIEEEAIEIMNNKLPPTGNYPEDSFYSSHTVQPSFFCQEVVLAREHSIREHMPFLYKILLVTLRLPPGNNNKVIEDNNLDPYEEELLVREGIQYESAVTLSTQSKSKKVAATICSMVAFSRNRRINGLQLFNSVRFLGAGITERMDQYFHLLSLTSSRQTALSALRTLLTVDSRELRNSMAIKESSPIGPSICIDNLDMEEQVHNHSVGHCSMMFHGTWGYIHHPNPNLLKLLDPSQLRLSLYYDTLSKVPTMKITLAMFMPSPVEEVHFEAVLKSQIARVMAQYIAKPAHPGIATNPPPVEKIDPSPPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQG6</accession>
    <name>A0A5B0NQG6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012687</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQG6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1090754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="99" mass="11003" checksum="EE4FE3B97884442F" modified="2019-11-13" version="1">MPINEAMPLDEAGADAAAGSRVSWWFGCDCVPTEVELAGLEKNREEYASRPAEDRAKPRWETACSTALRVSLHRLYYRLSSIDMSRQYPTGYRVGSSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTJ3</accession>
    <name>A0A5B0NTJ3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027034</name>
        <name evidence="2" type="ORF">PGT21_034207</name>
        <name evidence="4" type="ORF">PGTUg99_008500</name>
        <name evidence="3" type="ORF">PGTUg99_016822</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000379">
        <property type="protein sequence ID" value="KAA1092022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTJ3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="61" mass="6727" checksum="7B9221DD93FF1255" modified="2019-11-13" version="1">MPIRFRSTLPTSSTNSRLPISSVVFDRIFDALSTGLVGSRGFSFISRIVRVDWLGSAHGWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NW49</accession>
    <name>A0A5B0NW49_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018514</name>
        <name evidence="3" type="ORF">PGTUg99_026013</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NW49"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="123" mass="13415" checksum="778A309657B805EB" modified="2019-11-13" version="1">MSRTSNLEKPQEPLDPQETPLVVVNRRIVGFYTGLLQAAPQDATDPRKPLVSIGAVSRPSETARRNGPRTVHFNPEASETLGSSQDRPQDRPFDPRTSKPSEGPLDGPDLTVPIFVPGLHQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0P8</accession>
    <name>A0A5B0P0P8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_003852</name>
        <name evidence="3" type="ORF">PGTUg99_036356</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0P8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137609" description="Secreted protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1093940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13702" checksum="117CDC18077DA877" modified="2019-11-13" version="1">MLFSPSCHATFILTVCVFLGSVDCSPLKWDGFLVHDGVRIQPNQVDLQAREIQQMAAHDVDIGRPVPPQPLDLIERPVINNQLGAGSEEDPVQSEIHQDAESSVANPRRVQEPHEDASTSGPRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0U9</accession>
    <name>A0A5B0P0U9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">26S proteasome regulatory subunit RPN3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RPN3_1</name>
        <name evidence="7" type="ORF">PGT21_005655</name>
        <name evidence="6" type="ORF">PGTUg99_035567</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0U9"/>
    <dbReference type="OrthoDB" id="1713558at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000009">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013586">
        <property type="entry name" value="PSMD3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057985">
        <property type="entry name" value="TPR_PSMD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08375">
        <property type="entry name" value="Rpn3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25573">
        <property type="entry name" value="TPR_PSMD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="5">
        <location>
            <begin position="288"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="507"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="527"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00075103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1083520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1094000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="542" mass="60783" checksum="E041C86AF0CD5873" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4G9</accession>
    <name>A0A5B0P4G9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61</fullName>
            <ecNumber evidence="2 9">2.1.1.220</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TRM61_1</name>
        <name evidence="14" type="synonym">TRM61_2</name>
        <name evidence="13" type="ORF">PGT21_016518</name>
        <name evidence="14" type="ORF">PGTUg99_035627</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>adenosine(58) in tRNA + S-adenosyl-L-methionine = N(1)-methyladenosine(58) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10365"/>
            <dbReference type="Rhea" id="RHEA-COMP:10366"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="2.1.1.220"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.220" evidence="2 9"/>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4G9"/>
    <dbReference type="OrthoDB" id="1925287at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.20:FF:000009">
        <property type="entry name" value="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000247">
        <property type="entry name" value="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF2">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="12">
        <location>
            <begin position="74"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="276"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1096425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1132008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="401" mass="44560" checksum="B9609F6C17C9DBAA" modified="2019-11-13" version="1">MTQAEEARPSSIFDIRSGTIQAGDTVIVYQSRDNLTAIVVTPGHQLNTRYGEFPHSDMIDIPFGSKCPSRKGNGFTYLLRPTPELWTLALPHRTQILYHPDISFITSQLNIKPGSYVIEAGTGSGSFSHSIARTIGPGGKLFSFEFHEERHTKAAAEFQAHGLLSSVGGPIQLAHRNVIRDGFGDLDVKVDSVFLDLPAPWDALEESKRVMNRSQLSRICCFSPCIEQVQKTCQTLESLGFSDIIMFETLVRTHEPVSLTMPKVDDAVRRIKEVEMKKQARRERQISESKQRRQVAQKPSSPEPASDERNTKRGLDGADATEEPELKKPREEKCNGSPVCAKSEEMDTVMVEDTKPVVTETDQALPEVQQTVMLETSKPAGMTRGHTSFLTFAVLLPLPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA95</accession>
    <name>A0A5B0PA95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA95"/>
    <dbReference type="OrthoDB" id="10291719at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="412" mass="46904" checksum="20456905733B17EE" modified="2019-11-13" version="1">MDPGIEPRPPIHGLFIAIGQLERYGREAPRRRRRDISEKSLSSREIDAGKALLVEVQTRSLPTLQRQLHGLMRSLATGPLNEEESESDRKITNALEFALQLGATLNLIDRSLASLASAPISEELGFTCKIDHEYGVLKRYKCIDLVQKFDRLLSREFANLLGLYFSLIELYHSNIHFPIPAFLSNLDLEVPRLQGQIIKNTTEAWRSIDAIIRSSKGSDFSFLQQSWREDNTRLNICLCDLSESTLELENDRSQENVLLNLDLMHRIQRAKPLALLGEEFLDKLMDTYYAKTSFTLSPSVSSYDMGCLKKETSGLCAHILKIVKSFTGSGPANEDDLHAEMQKILVWSKELSRHFDSCMTLFAFFLIPDPSRIDPPFPEDLLRSSFFELRSQFHLALEQLLAADRKLETLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBI2</accession>
    <name>A0A5B0PBI2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034381</name>
        <name evidence="3" type="ORF">PGTUg99_017008</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000176">
        <property type="protein sequence ID" value="KAA1125649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBI2"/>
    <dbReference type="OrthoDB" id="10605551at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="595" mass="69035" checksum="EB703AE23F07E5E7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEZ4</accession>
    <name>A0A5B0PEZ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004496</name>
        <name evidence="1" type="ORF">PGTUg99_009913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000482">
        <property type="protein sequence ID" value="KAA1070944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEZ4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="221" mass="25589" checksum="5FCC950674A7BFBE" modified="2019-11-13" version="1">MPSLPSLLALRVMKQKLVDLFQLCFFFWKTRLLSLKPPQLSLVQQHTRHTGASPSWQLMPDDKRWLTMRIDTAALLGLKQPHSSKLKSRMTLALLLKRRMVRMLAHSIADDFVSYWCLVVNWLDLFITSQPERFPRKDHIGCSGLRREDNYNLNSQLGQIHVACGVLVQLPLDWKTSHSRTKRRKILDGHHLDPKLHSAANLSTAHVVNRVFYLILSKKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFC6</accession>
    <name>A0A5B0PFC6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFC6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="412" mass="45036" checksum="FBDA525457D82420" modified="2019-11-13" version="1">MLTSGPPHRGSSICTCHWQLSEDRLGTPRSGFQPLLSQNNHQHQAHHCQIVVIASSAENRHLAAKWVCPRPYSEGQQLTDVISSSANNQQATAPPPSSPQAPNNSNPSASADGIELVPPSTQPPLENERTWVHLSTSMRHPPSSPQAPILLLQRPQPPEAFCLGRRNRPSPPLHPAAFAKRNDPGRPIDLGDAPPPPFSPQAPNHLEPSASADGIDHLPPYTQLPLQNETTRVDPSTSATHPPRFSPQAPNHLEPSASADGIDHLPPYTQLPFENETTRVDPSTSATHPPRSLPRPPTTWSLLPRPTESTISPPTPSCLCKTKRPGSTHRPRRRTPPPFSPQAPNHLEPSASADGIDHLPPYTQLPFENETTRVDPSTSATHPPFSLQAPSLPLDRFFLSGWRVDWFFLSGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFT9</accession>
    <name>A0A5B0PFT9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFT9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="662"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="579"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="668"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="683" mass="77114" checksum="C13364FE300F9E1E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PK80</accession>
    <name>A0A5B0PK80_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_002068</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PK80"/>
    <dbReference type="OrthoDB" id="2506114at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="69"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="707"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="771"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="821"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1037"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="782"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="844"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1038"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1062"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1075"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1089"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1106"/>
            <end position="1116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1100938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1124" mass="125718" checksum="EB8B718A9EDAC2FE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNI1</accession>
    <name>A0A5B0PNI1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_010089</name>
        <name evidence="3" type="ORF">PGTUg99_011299</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000484">
        <property type="protein sequence ID" value="KAA1070141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1103215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137793" evidence="2">
        <location>
            <begin position="18"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1103215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="175" mass="19037" checksum="79B91C7B3EC3B3E0" modified="2019-11-13" version="1">MISIWLLFLLVSQYVAMEGPHPRPSSIGPPVPSDRAGIRETNPYISLPAAHTIDIRSDEGCSSITMQEPREKEIELHERTAQQQAHYGNTSGPKDLSVTMNQEQIHAKGIPMAATSKTNVQVREQTDLSGNSCDGLCNQIIGLSIFSIAVLIALAIIGKSTGNGVLFSSYIRRHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PPW6</accession>
    <name>A0A5B0PPW6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_012524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103281.1"/>
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    <dbReference type="Proteomes" id="UP000324748">
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="95"/>
        </location>
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            <end position="35"/>
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            <begin position="74"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="9893" checksum="68BC862EBD25D54A" modified="2019-11-13" version="1">MAFLNNVKVQAPSSQAKASSPGSTTAGPNKFTQLHHSWPKPFPPSLAMTNSPRSTRSSSSTTSGANHLIHFINSPAPSSFPETASSASSSDSCNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRA9</accession>
    <name>A0A5B0PRA9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010944</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRA9"/>
    <dbReference type="OrthoDB" id="2504815at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022661245" evidence="2">
        <location>
            <begin position="25"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="363"/>
        </location>
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        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
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            <begin position="65"/>
            <end position="81"/>
        </location>
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            <begin position="143"/>
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        </location>
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        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
    </feature>
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            <end position="203"/>
        </location>
    </feature>
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            <begin position="218"/>
            <end position="230"/>
        </location>
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            <end position="247"/>
        </location>
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        <location>
            <begin position="329"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="398" mass="41932" checksum="71A91990BFD2E569" modified="2019-11-13" version="1">MPSSRSLGAAILLGVMPLIQDSYSSPVGGRWESSRALGISSSVHHGMSANPIVTRKKRSHLIRPRSSQAETISQRVNQILGSSDIVPPDALPEPKEKPDAPPTGASGPEKAVPPTAEHKPQDKPTPDHEGNPFSQALGPNPPAKKDNGPGHEELIPHPIDDPSAPGPNPQPDGKPHEPHGDDHNGHEGAGGPPPKKDGPEEHPPPPPPPADSHKPVGHPEPNPLNPPHPPNGDEHPKTGPHGPEKAKTQTTEIAVIAGIRIERVDFPVPDSTPTHPPAPADANAHHGPKENPLDEKDHAPPPPVASGHDDPKSHPSEKDHPPPPAPADAHAEPKDHPPAPAAEHGHDHPAEKDHPEPKQVEIPGVIMITNDSKAGKTTEAGPRAIRITTDKLKLELLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSD9</accession>
    <name>A0A5B0PSD9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_016745</name>
        <name evidence="5" type="ORF">PGT21_016971</name>
        <name evidence="6" type="ORF">PGTUg99_007473</name>
        <name evidence="8" type="ORF">PGTUg99_010183</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 10">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000448">
        <property type="protein sequence ID" value="KAA1078655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000108">
        <property type="protein sequence ID" value="KAA1130499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSD9"/>
    <dbReference type="OMA" id="FIEMADD"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21608">
        <property type="entry name" value="RRM2_NsCP33_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048289">
        <property type="entry name" value="RRM2_NsCP33-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1065968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1078655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1103398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1130499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="179" mass="17532" checksum="E40BA2D199409DBA" modified="2019-11-13" version="1">MSAAPAGGKLFVGNLAWTTDSGSLKSAFGQFGDVIDAIVMQDRETGRSRGFGFITFKEEADAPGAIEALNGQELDGREIRVNYANSQTGGGGGGGQRQGGGGGYRGGYGQSGGGGGGGYNSSYGGGGYGGDQGGYGGNSTHQSYGGGGGGYGNGGQGQGNWRGDQQNSYNAPSGGGGAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT51</accession>
    <name>A0A5B0PT51_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013264</name>
        <name evidence="2" type="ORF">PGTUg99_007025</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000314">
        <property type="protein sequence ID" value="KAA1104947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT51"/>
    <dbReference type="OrthoDB" id="2107880at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20180">
        <property type="entry name" value="UQCC2_CBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="132" mass="15343" checksum="86D5FC83C1520E60" modified="2019-11-13" version="1">MASSQRLMKIQSELKQIQRTWPLDPLRINQPELQISSSISKAIERVFSNNYKNDQSVSSQELLEQKTLEGAEKMLSSLENLSNGSIARQYPFPKSMRNPASFPGHYEELNEGVQRAIRGESLPWYRRWIRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTC5</accession>
    <name>A0A5B0PTC5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_004647</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTC5"/>
    <dbReference type="OrthoDB" id="434783at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="10"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1103922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="11899" checksum="0EF53AB00B70FC93" modified="2019-11-13" version="1">MMPKADSKTREVVNNFISGSIGGCLGTMVNTPFDVVKSRIQNTVVLPGEKPKYGWTYPAIVTIAKEEGLGALYKGFIPKVLRLAPGGGVLLVVVEVVTGYFRKQLGPPYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUJ7</accession>
    <name>A0A5B0PUJ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022544</name>
        <name evidence="2" type="ORF">PGTUg99_028942</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUJ7"/>
    <dbReference type="OrthoDB" id="10478532at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="133" mass="14907" checksum="68CDA82713DF3803" modified="2019-11-13" version="1">MPRGKRHRVNSSPSPSVSSTPAQSQEPSRRKKISSRTTGNRDNDLENDQTTDADATTPGGTQNTKIELTDEQELDKARIKHVNQLSSNYSYFDPPELSEHLDKHRRKMIAFPCATPGFTGQHMITLQQTSQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVP3</accession>
    <name>A0A5B0PVP3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032494</name>
        <name evidence="2" type="ORF">PGTUg99_015992</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1099004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVP3"/>
    <dbReference type="OrthoDB" id="10462312at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="179" mass="19894" checksum="BF6278B8B8841B14" modified="2019-11-13" version="1">MRCRYFAGNADIIGRHRLNLPPEPPDMRRDSGGFVVSLCPEFTVTQGQVTPTPSATPSNDETALKRRRHPILTNSTATRTQIQFVNSIHLAWNRSIGIESDELPSTVVHCHQDSTVIKQTKRLVSLTLRNPTPSLVVCTDQAKSCWTTRFTAIASPLSSKGTHQSKETQKHQAPKNNQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PWB7</accession>
    <name>A0A5B0PWB7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028508</name>
        <name evidence="1" type="ORF">PGTUg99_010240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000349">
        <property type="protein sequence ID" value="KAA1097234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PWB7"/>
    <dbReference type="OrthoDB" id="2496784at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="387" mass="41844" checksum="316349612C849932" modified="2019-11-13" version="1">MSVDGKQKSTSKHTQSRALDSLRVCKWLVLVDPWTVTSNSNRVRLNKTFDIPSFRNLASITWYLISLTSSWKYVLQYSMVLAQVSPRTSKHSASMFFAVGLVHLLCLSHPIGSASIKLLDSPDHSIDSTREGHVQAVKSTAHISRRSDTGLSPAVANSGCYNYLKGKDGCAYANPTNNCARKMSSMAVQLFAMGPPPGNTSSHLDQSSLPGEKALLKRNENQTWSAPTPENTTSFLATNSILSSNSSSNSSIFLGNTSLPLYNNSESSICGNYDGEEQMGVCLWSGPASGTPDSNGSGWVSSNITANCHKEVILQRSGSNSPMIVARVLEGCSFNTEELATGCSQIYVTKKVFMALNPSPTELEKGALEDTITWDFNSRGNIFNSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXI5</accession>
    <name>A0A5B0PXI5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012677</name>
        <name evidence="1" type="ORF">PGTUg99_001223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000350">
        <property type="protein sequence ID" value="KAA1097166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXI5"/>
    <dbReference type="OrthoDB" id="10311898at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="176" mass="19276" checksum="72ECA7502B0BECAC" modified="2019-11-13" version="1">MRVIQQANVDNFDALLANPSAITKDPNLLTMTRKRNKTTPKGTLSYPSAVAQDLVHHLVHSFEVFYGELLGPQAKFPVAAFFGVEQATIIVGSMDQICSRGSQNMGLMELLMGVQCFPGQLESLNNAIIQWMASKVYLSHLLQTANLTRFIKSEGLQVQEAMAAKPAALQSQAKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYP5</accession>
    <name>A0A5B0PYP5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026852</name>
        <name evidence="3" type="ORF">PGTUg99_013730</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1120901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYP5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="57" mass="6625" checksum="F7C1ED62AAD54D4F" modified="2019-11-13" version="1">MEEKLAREKVIADTRETEEEQDEEEYDDEDEDEAKAKVETNAEDEAKATGDNMDEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZ35</accession>
    <name>A0A5B0PZ35_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal RNA-processing protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SKI6_1</name>
        <name evidence="12" type="synonym">SKI6_2</name>
        <name evidence="10" type="ORF">PGT21_030062</name>
        <name evidence="11" type="ORF">PGTUg99_018178</name>
        <name evidence="12" type="ORF">PGTUg99_034086</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core complex (Exo-9) which may associate with the catalytic subunits RRP6 and DIS3 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric base ring of RNase PH domain-containing subunits and a cap ring consisting of CSL4, RRP4 and RRP40.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000169">
        <property type="protein sequence ID" value="KAA1127288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZ35"/>
    <dbReference type="OrthoDB" id="437922at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11370">
        <property type="entry name" value="RNase_PH_RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000004">
        <property type="entry name" value="Exosome complex component Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="37"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1106163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1109215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1127288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="292" mass="31832" checksum="F4FD15BFCB20BC52" modified="2019-11-13" version="1">MSRVELLSSGGLRLDNRRPYELRSIDFNILPNPPVGCDSVARVEHGLTKLIGFVSGPRDSVESNRTVATTLGNPTSNSASSNLNNNNNNQNGSISVHISTTPFSSIDRKKIGRSDKKFADLAQAIQNTFEPVVMLHLYPRSTIDIYVQVLQQDGALLQAAINVTTLALIGAGVSISDYVLSVSVGALSTPSCPLLDLTNSEQTDLPNLTIAVLPRTQKLTLLQLDTRINILTFESLLEIGLEACLVLQLEMDREVRRWATLLNSSIHNSLDYKSHSAQGPVADQNPQIMMID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZ95</accession>
    <name>A0A5B0PZ95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZ95"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="99" mass="10884" checksum="99E19DA68313F259" modified="2019-11-13" version="1">MTSSYHTTKQMTNTTKTNSPPQPNSPLNNQTTPDYSTSKQTRQQPPSNDSRPSPPAPYPHSSTSVQTSHPPTWESTRITGLINYTKLTRPSDSLAMIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q323</accession>
    <name>A0A5B0Q323_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017278</name>
        <name evidence="3" type="ORF">PGTUg99_028476</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q323"/>
    <dbReference type="OrthoDB" id="2500732at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="747"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="931"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1033"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="749"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="934"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="962"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="993"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1071"/>
            <end position="1086"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1133" mass="125195" checksum="B4C0D19DBBF10469" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5H6</accession>
    <name>A0A5B0Q5H6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Small nuclear ribonucleoprotein Sm D1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">snRNP core protein D1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_007879</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the snRNP core protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5H6"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097525">
        <property type="term" value="C:spliceosomal snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01724">
        <property type="entry name" value="Sm_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000016">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034102">
        <property type="entry name" value="Sm_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="78"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="156"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="159"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1108317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="198" mass="21651" checksum="BE7975F2516BA69F" modified="2019-11-13" version="1">MSCGKSVCSTGGAGKKPRRGCPTSPSHRAPGQASPHHRSIVRQPTLEADKSLETTQSTPHSKTTQPFCRRVLSLFRMKLIRFLMKLNQETVTIELKNGTVVHGTISGVDVAMNTHLKAVKMTVRNREPVQLDSLSIRGSNVRYIILPDSLPLDTLLIDDAPKPKKKKDGDARGRGRGRGAVERGGRSRGRGRGRTRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q648</accession>
    <name>A0A5B0Q648_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012734</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q648"/>
    <dbReference type="OrthoDB" id="10304069at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="518"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="564" mass="61555" checksum="A6F6608D528C439E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6M1</accession>
    <name>A0A5B0Q6M1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6M1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="79" mass="9058" checksum="FB2FC76A69158C0F" modified="2019-11-13" version="1">MVALMEPDNLEGDESTERMSVTNCSYARQIEEAQNAISQRSMERNIPYYAVPDALVIWNIRRMRLESMAGKPNTSADYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC60</accession>
    <name>A0A5B0QC60_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030229</name>
        <name evidence="3" type="ORF">PGTUg99_037398</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000001">
        <property type="protein sequence ID" value="KAA1139219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QC60"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1139219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="148" mass="16070" checksum="A069722A527BF6D9" modified="2019-11-13" version="1">MLLTTESDRADYPRIDHYGSTDNQKLLTPAQSTGYVPPIYTRRALCKNFHLCSLPSHSSPSQSPQLDSKSPATCLSKSPLRQKPNIAAAIVTLSRNALPRPLYVCDDPSNTPKFSGYCTGVTSRATSQDPSAAAPDTLVMTRATRQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QD41</accession>
    <name>A0A5B0QD41_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein transport protein SEC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SEC9_4</name>
        <name evidence="7" type="ORF">PGT21_035936</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNAP-25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QD41"/>
    <dbReference type="OrthoDB" id="18679at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15857">
        <property type="entry name" value="SNARE_SEC9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15886">
        <property type="entry name" value="SNARE_SEC9N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000043">
        <property type="entry name" value="Protein transport protein sec9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000075">
        <property type="entry name" value="Related to SEC9-protein transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF9">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="6">
        <location>
            <begin position="255"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="178"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1111039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="318" mass="36094" checksum="44D6E122C5E765D4" modified="2019-11-13" version="1">MFSRKKAVEIPPVEEPKARAPPVNRNLLSSSRNPAGPSSSGRPMANDRFGNPDANRQELFKGARPPNRSFVDRDMDGPPGSSGSQLDDEDEEVEAIKQQIKFTKQESLSSTRNALRIAREAEETARNTMNRLGEQSDRLANTENHLDMAKAHAVRADDQQKEIVALNRSIFRPTFTFNKKGKRDAHEQKLLSRHEEEKAEREEVRRQQYESRERTAETLKALDKGANQPKGAPGGLRSRLADKARYQFENTESDDELEDELDDNLNEISGLTSRLNLMSKTMGTEIDSQNRKLEGMSTKTDRLDNRIHGQTERLKRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QD82</accession>
    <name>A0A5B0QD82_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QD82"/>
    <dbReference type="OrthoDB" id="2499245at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="257" mass="28339" checksum="7A3029140D1AFEC9" modified="2019-11-13" version="1">MDQVTQLRSARAGSHILAPNLATFFAPPKIAYQRLRAALGQAWGIRRLANVKNKWVQQANDDNNGLYTAYYHFCQLYDNLGASLNDPAGDGASRSAMELVQNFCGAMAGLAFICTKNNMKAKQAKLDKLRTVHHVQHVQMLANLLIFGPTAAFTSLHKGPHMTLSNANALLELGSSVLKQKIALGITTPMPHSTLDNLRIHFLNFLIKMGFNQLDNDGVPQVNWKDVHASFYNDFSPSVIAGIFSQDIEIALAHRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHE4</accession>
    <name>A0A5B0QHE4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHE4"/>
    <dbReference type="OrthoDB" id="5562676at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018786">
        <property type="entry name" value="Mit_KHE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062">
        <property type="entry name" value="K+-H+ EXCHANGE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10173">
        <property type="entry name" value="Mit_KHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="15586" checksum="61DC5F8873BD1376" modified="2019-11-13" version="1">MLLTKPSIRLIALPLSKRSNTLIYFYAQQLPSLADQKSSEASLPSHASFNLSPSSLIKKMTDTGTKQWNKLANSAHGSWKLKIYNTGERMMDKIPFEEWALKSIDPPHIPHKILPSESHNEKNQTGLKEAQKTSQVVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHP3</accession>
    <name>A0A5B0QHP3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007913</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHP3"/>
    <dbReference type="OrthoDB" id="2514281at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="268" mass="30663" checksum="2BF4C46B576FD80C" modified="2019-11-13" version="1">MGSHLGTQGKENIIKALQNRRPAVKKIIDTFNGLYEQFQQKYPEQHLSDAHDHPLTYDDFVEWEMDHPFWNDGLYHHTEAPWSIDPDVKTGINCILVLRRVQEEFELIAQELARAVGWAIATHSQLTETIVHITTRINLVKSDPNVATDRFDDLSFGVVSRLGKLELVQEELKLSLIDHGKLIEDWNDQVNSLWRQCQPSGNSAQFQQWTELIDKVKVDQSTVFPTLSAFSQPQNDVEDALEEAILDVGLDDGEDADEEWMSEGEEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIC0</accession>
    <name>A0A5B0QIC0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fungal-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIC0"/>
    <dbReference type="OrthoDB" id="312874at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040976">
        <property type="entry name" value="Pkinase_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248:SF2">
        <property type="entry name" value="FUNK1 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248">
        <property type="entry name" value="FUNK1 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17667">
        <property type="entry name" value="Pkinase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal-type protein kinase" evidence="1">
        <location>
            <begin position="232"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="660" mass="76079" checksum="21563FB693F9286A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QII4</accession>
    <name>A0A5B0QII4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QII4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="15039" checksum="9B7AD3B40F13F5F0" modified="2019-11-13" version="1">MRFEIVSHTVFQALVIESFIVAAIVSAAKVRIGETSISPQAAEPQTFETQLSSLLGKPCRACQLGTIDRIIHQPGQNCWHIMRCEHGRPVGPCMGVKPVAQPHCDRCFANIAIIGICEMAEADGTHTNVPECPSQCLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN98</accession>
    <name>A0A5B0QN98_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_014113</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN98"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969:SF69">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023016521" description="RING-type domain-containing protein" evidence="7">
        <location>
            <begin position="23"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="117"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1114573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="236" mass="26301" checksum="358F7C1B484E1966" modified="2019-11-13" version="1">MNLCTMSATVLLILKLVATNLAMNVPQSSERLGFRKINPNLRNQESKYPLGEGTSISQLRCQLAHSARYDWEGPHNSHQDLEMGLAASSSSSHEMQPIQTSKESKSLGALKPEKEDCAICLEEMKADTDEVFPWPECGHTYHKICIQPLVDSKGNCPKCRAPFPKEISTTDQVSVPPTNIVRRVWTALDLRTPKGRFLCFLIFIECAFLSLLFHAMISASPTRQSTHAPAPKSPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNE1</accession>
    <name>A0A5B0QNE1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNE1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="109" mass="11448" checksum="048F105299124BA1" modified="2019-11-13" version="1">MPRGKQSRNSQITSSSTKGTNTTGPPNDQPTMSSSGDSDSNSDSKSNIETHKGLSQDPPPTAPTPTPSNKSTKNTRNLASNQDSEASTEPASKKRKTTSDLSGSVMPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSB0</accession>
    <name>A0A5B0QSB0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSB0"/>
    <dbReference type="OrthoDB" id="10309016at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022898673" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15033" checksum="E89AB66A56DE16EA" modified="2019-11-13" version="1">MQFSTLFVLSMMLLFLTHETCCFSWVCLGEGATVCSAPVTKSEKSRNRDLKDFWVSDAKGAGTNLFTCDDVLPGGHLATRGWCCIFKSLKSVCMKINLLLQLQLTTDLPVLLRIPSPPQTSISVWLVITGYTNDPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWG5</accession>
    <name>A0A5B0QWG5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Capsular associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CAP59_1</name>
        <name evidence="3" type="ORF">PGT21_008788</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWG5"/>
    <dbReference type="OrthoDB" id="262547at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021047">
        <property type="entry name" value="Mannosyltransferase_CMT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34144:SF1">
        <property type="entry name" value="CAPSULAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34144">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11735">
        <property type="entry name" value="CAP59_mtransfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="611" mass="69248" checksum="1C9691F380C5CB62" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXD1</accession>
    <name>A0A5B0QXD1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
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            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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            <begin position="23"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12765" checksum="4A5C4071089F0071" modified="2019-11-13" version="1">MQLSNVLPIFLVFFLQGASTYAQAFGCKDNYQVPNWPHSGCVKMSGMKQELADVIPAHWNNKASSYECGSDANGYLTVACCAHEDDMPKQLPSDTFINNCRKLDGTQFTWPLPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYE4</accession>
    <name>A0A5B0QYE4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYE4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="7816" checksum="BD10F58DDF94AC6F" modified="2019-11-13" version="1">MPVLVSIPIKTADIFDRVRLPTSSSGFDRIFRASMTNRVIFLPSAESIKVIGQDQSALGCSASRGRFQESGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1T7</accession>
    <name>A0A5B0R1T7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_012936</name>
        <name evidence="3" type="ORF">PGTUg99_010044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000413">
        <property type="protein sequence ID" value="KAA1086140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1T7"/>
    <dbReference type="OrthoDB" id="10454383at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033845272" description="Secreted protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10551" checksum="773239F91124900F" modified="2019-11-13" version="1">MRLLLIVAALIPFAYLQEPGRQVQRDGTGFVDRLGKRGSGDPIAIAKGPPAKRKNLPLNKPNKPKPITPPSHRPLKNNNRLLYSAADGYYTEMFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2V9</accession>
    <name>A0A5B0R2V9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2V9"/>
    <dbReference type="OrthoDB" id="10510227at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023020833" evidence="1">
        <location>
            <begin position="25"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="579" mass="68615" checksum="0686E3EB63D57CCB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R615</accession>
    <name>A0A5B0R615_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005623</name>
        <name evidence="3" type="ORF">PGTUg99_017672</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000244">
        <property type="protein sequence ID" value="KAA1120435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R615"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="109" mass="11485" checksum="6C55A4794FA11D18" modified="2019-11-13" version="1">MLRAQCPHSPRACRKLRELSQAPRAAKCELAKHAFTARRRLDNGHSTDLMHDDVMGLSGKPCPPPAPGFPFPSGGQNLPVSKTATDVLNHGEGIEGVGGVPSPTLSPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RFT3</accession>
    <name>A0A5B0RFT3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein PBN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_022948</name>
        <name evidence="12" type="ORF">PGTUg99_023737</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for proper folding and/or the stability of a subset of proteins in the endoplasmic reticulum. Component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase GPI14.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PIGX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RFT3"/>
    <dbReference type="OrthoDB" id="5546453at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013233">
        <property type="entry name" value="PIG-X/PBN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040039">
        <property type="entry name" value="PIGX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08320">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00780">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1083020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1124048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="247" mass="27218" checksum="328FFD3033215C35" modified="2019-11-13" version="1">MVGLTTELSERVYGFHPSLRTTVTLSTDEVEGLKECAVGLVFAGLPGTVIVDRYQLRALYEEGRLSEPRVVPTKDGLLDLLVWTDPDLEGPASRPDPPGLGGLMVPLPLPFTGSLSILVPLHLRYLPPILDPKTDRRPVREVLIQPPSLVKWCNQSTPIASETSLPAISPDEIRLLSEFFSSPFQPLQGLPTVDAHLHPSSPIRLSVPVGVQHDEWPVLIISTAAVWLAFAWICSCLYYKKHLPIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJE0</accession>
    <name>A0A5B0RJE0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 16">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="16">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">INO80_1</name>
        <name evidence="21" type="ORF">PGT21_018097</name>
        <name evidence="22" type="ORF">PGTUg99_008185</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">21-0</strain>
            <strain evidence="22 24">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="16"/>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000177">
        <property type="protein sequence ID" value="KAA1125499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06503">
        <property type="entry name" value="ATP-synt_Fo_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18002">
        <property type="entry name" value="DEXQc_INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22541">
        <property type="entry name" value="SP5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000022">
        <property type="entry name" value="Blast:Putative DNA helicase Ino80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001445">
        <property type="entry name" value="Chromatin-remodeling ATPase INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001304">
        <property type="entry name" value="DNA helicase INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031047">
        <property type="entry name" value="DEXQc_INO80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="7 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13 16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="22" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DBINO" evidence="20">
        <location>
            <begin position="578"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="854"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="1429"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="183"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="357"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1570"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1608"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1638"/>
            <end position="1657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1673"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="481"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1613"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1643"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1708"/>
            <end position="1727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1090927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAA1125499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1762" mass="196173" checksum="7EA8916AE7A9D96F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RKA7</accession>
    <name>A0A5B0RKA7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">mitochondrial processing peptidase</fullName>
            <ecNumber evidence="5">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGT21_035902</name>
        <name evidence="18" type="ORF">PGT21_036729</name>
        <name evidence="20" type="ORF">PGTUg99_023251</name>
        <name evidence="19" type="ORF">PGTUg99_035597</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="20 22">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins. Preferentially, cleaves after an arginine at position P2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000237">
        <property type="protein sequence ID" value="KAA1121924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RKA7"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000002">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="15">
        <location>
            <begin position="56"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="16">
        <location>
            <begin position="206"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1106525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1115465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1121924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1126276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="480" mass="52895" checksum="1E69BE6046C56822" modified="2019-11-13" version="1">MSASRFLLSKPSQALLNQATRRIPVPSTSVRSLATIAPSSTFPQTRISTLPNGLTVATEPHPHSQTATVGIWIDSGSRADKHGGTAHFLEHLAFKGTQKRTQHSLELEIENLGAHLNAYTSREQTCYFARSFSHDVPKVVEIISDILQNSKLEEGAIERERSVILREQEEVDKAHEEVVFDHLHAVAFQGEDLGKTILGPKQAILSIKRPDLVEYIKSNYTADRMVLVGAGGLEHEALVELASKNLGNLPTSSSPIPLGGRGQIKPTGFTGSEVRIRDDTMDTINLAIAVEGVGWNSPDLFPMLVMQSIFGNWDRSLGSSPLMSSRLSHALSSNNLVNSFLSFSTSYSDTGLWGIYMVSENLTNIDDLVYITLREWQRMSTAPTEIEVARAKSQLKASMLFSLDSSNNIADDIGRQLVTSGKRMTPQEIQIAVEAVTPETIRRVAQKYLWDKDIAVAALGRVEGLLEYNRIRANMSSLTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RQT2</accession>
    <name>A0A5B0RQT2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027506</name>
        <name evidence="3" type="ORF">PGTUg99_007011</name>
        <name evidence="4" type="ORF">PGTUg99_029659</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1120881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000169">
        <property type="protein sequence ID" value="KAA1127183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RQT2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366659" evidence="1">
        <location>
            <begin position="17"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1127183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="99" mass="11045" checksum="AD6FA634EDAC7869" modified="2019-11-13" version="1">MKLIIIGIMLVTFASSDVIKPPVYPLKWGCDKFQVNDVDFSFPGCEVEFEVNDVPKDARDCPKKKLRRCCNMLASKAVSYGGDCVNAPKRGHIHSPDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RRR5</accession>
    <name>A0A5B0RRR5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001442</name>
        <name evidence="2" type="ORF">PGTUg99_009605</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000145">
        <property type="protein sequence ID" value="KAA1128012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RRR5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1128012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="95" mass="10560" checksum="F0FF6A91BB479368" modified="2019-11-13" version="1">MWVGHNTGSGKSWIGLGDTDTKFPRGSYRIAPADPNHLINRDWIGKTIRSRYTVLGHISIRSPINTDPGIGSVTSLYVVNSNFYVDPHPLMHSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S0I9</accession>
    <name>A0A5B0S0I9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028696</name>
        <name evidence="3" type="ORF">PGTUg99_025751</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S0I9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366648" evidence="1">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="120" mass="11227" checksum="1F82F2390E3C1482" modified="2019-11-13" version="1">MQVSLTLCFLVLLTISGRGLSMWELTGTADLPISYDAIQGQSAGSHTSAYRHTHKPALCVTRRCGTRGKDKKKGGTSGPIYTGGGGGCGGGGCGSGGGGGGGCGGGGGGGCGGGGGGGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1P1</accession>
    <name>A0A5B0S1P1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_028188</name>
        <name evidence="9" type="ORF">PGTUg99_023835</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the methyltransferase superfamily. METTL16/RlmF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1P1"/>
    <dbReference type="OrthoDB" id="514248at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017182">
        <property type="entry name" value="METTL16/PsiM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010286">
        <property type="entry name" value="METTL16/RlmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393:SF0">
        <property type="entry name" value="RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05971">
        <property type="entry name" value="Methyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037350">
        <property type="entry name" value="Mtase_ZK1128_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="425"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="396"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037350-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1081045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1131205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="56438" checksum="37B2CA804CFA0A47" modified="2019-11-13" version="1">MHPDNLYAHQPPDFDRLIKSDPTIKRSSLLVSFLQDSDRTSNTVFKDPEKLRQLTCALLLSDFKLKLSLPPDRLCPIIPGRLDYCLWLIDVLKASVELSDGGAGVLVIDVGTGSSAIYPLLLTQLLKNVRVIATELDQSSYDSAMRNVTQNDLGNRITLQKTSSTDPTILPIASISQEPDQMISITMCNPPFYSSQDEIDALRAKKDAPPEGVCTGSEVEMVYPGGEVGFIEKMMRDSLIIGSQTRWFTSLCGKYTTLSPVVELFKSLGGNNYAISELIQGRTRRWVIAWSWQYYRLPDNVARALGPPGPRLKGSSQLLPLSNHLEYYISLDVLVVSKRHSLIDQITKTLELIEKCRFEVLQCEPTYMWEVTLFEKNWTRNARRKRKFAAPETKISEATSGTLETDPSSHERLDKDPILKVKLSVNQYQPTPRPEDHGAKDEKTISPRNPSSSSSDTITPAEERIGLKIEMDWLFGQDRKLFLSFWNHVLLKKSNAPSQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S512</accession>
    <name>A0A5B0S512_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029318</name>
        <name evidence="3" type="ORF">PGTUg99_025156</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1133040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S512"/>
    <dbReference type="OrthoDB" id="10287518at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138271" description="Secreted protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="17682" checksum="9D8E6894C4B382AC" modified="2019-11-13" version="1">MIAFPRLQTILLVIALIITLHPASVASAAKPAPATCDIYFFPSATADKYMCRTTAVDSKGKHQNLKCTLSKCSIKGGSWDIFAFENCLLYADNDKTKQPVVGAKPETVKAVQYHVVVAVSPPETYLQVFGRSNGKWYKCPVDPKKPDAINSQRAICTDCAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S889</accession>
    <name>A0A5B0S889_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002278</name>
        <name evidence="4" type="ORF">PGTUg99_035521</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S889"/>
    <dbReference type="OrthoDB" id="2496841at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474921" evidence="2">
        <location>
            <begin position="20"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1134351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="152" mass="15817" checksum="7DB39CFB43633951" modified="2019-11-13" version="1">MQHQIIILLLVSLAAFSQSANPQVPQVKLPDSFTCKAAVKGKETSHTFQAKDCTKAVDLLAKGKAPSAACGQCLLGLVGEDGNAFTPTHALDSNAILASVKNIADGCNNSAQSKPHNQKRDNKPSGNDSKSSGSAPPKIQVVLGLPKEKAQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S9L2</accession>
    <name>A0A5B0S9L2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PRE2_4</name>
        <name evidence="12" type="synonym">PRE2_1</name>
        <name evidence="12" type="ORF">PGT21_008876</name>
        <name evidence="13" type="ORF">PGTUg99_029418</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S9L2"/>
    <dbReference type="OrthoDB" id="37597at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03761">
        <property type="entry name" value="proteasome_beta_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000013">
        <property type="entry name" value="Proteasome subunit beta type-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF3">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 11 13" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1097498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1134135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="289" mass="32198" checksum="8161BAC1F916181C" modified="2019-11-13" version="1">MSLNGFLQELSTNEHRLAERECEEGQKEYALHHQDPGRTPVPRFDLPGVEDPVSFLRTHTDDRSADAKIKIKHGTTTLAFRFQHGIIVAVDSRATAGSYIASGTVKKVIEINPFLLGTMAGGAADCQYWETYLGIQCRLHELRNKSRISVAAASKYLSNLVYSYKGMGLSMGTMICGWDGAGPGLYYVDSDGTRLKGDVFSVGSGSTFAYGVLDQGYHWDLSVDDALELGRRSIYAAGHRDAFSGNTLNLYHVKEDGWHFINNYDFNKLHYDMAYGYEERLKKEAEVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SK05</accession>
    <name>A0A5B0SK05_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035963</name>
        <name evidence="3" type="ORF">PGTUg99_019254</name>
        <name evidence="1" type="ORF">PGTUg99_020602</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000474">
        <property type="protein sequence ID" value="KAA1073904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000003">
        <property type="protein sequence ID" value="KAA1138212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SK05"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1138212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="53" mass="6382" checksum="F1232C7340666D1B" modified="2019-11-13" version="1">MSWATRSMIDIEFYDRYRGGCSIESEEGYVPIFDPWRMAVKRGLLIWMRSMIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJ50</accession>
    <name>A0A5B0LJ50_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJ50"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="122" mass="13619" checksum="938E0EED7A4B388D" modified="2019-11-13" version="1">MPIRRVSTSRHTNYPSIPESPSNAARKRPISLALSPSLTCLISFIILKVRLSACKESVAIKFYRLNSFPLLFNCSIHLNNTKETLIGYSQPRYNRCIPGIGLQAKALGTIFLYLDSSIFAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKU5</accession>
    <name>A0A5B0LKU5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_000880</name>
        <name evidence="7" type="ORF">PGTUg99_003116</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1079914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKU5"/>
    <dbReference type="OrthoDB" id="1667110at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF38">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLASE 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="5">
        <location>
            <begin position="590"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="91"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="169"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="206"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1065497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1079914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="851" mass="96760" checksum="0B31DEDA9741B1C7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMD7</accession>
    <name>A0A5B0LMD7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMD7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="161" mass="17777" checksum="171CB298D60AAF04" modified="2019-11-13" version="1">MNLFLLEVDTPTTDLELKFIQHEVYSRSSRVTVNWVDLELNGVHLEVDLSSSRFTGDIQPQADRASAGGRSLIELSPRGPRAGHCPARGRNPLQSSQHRPRAESDSSSSSNAFGKPYSFDLELKSIQLEVDPPLSATMDLDLNRFQLEVELLSKGIGLTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPH7</accession>
    <name>A0A5B0LPH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000191">
        <property type="protein sequence ID" value="KAA1066847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPH7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="104" mass="11994" checksum="20E83D233CE8633C" modified="2019-11-13" version="1">MLSRQPPSIGLFLKTRRQKLRLQHPISAISILGTLTRPDLQLTITIIANKLTDRDNNDGLQRYTTAPPPVFEPEEKATRVQPQWLPVMVYIEGEGRGEKREKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPX3</accession>
    <name>A0A5B0LPX3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPX3"/>
    <dbReference type="OrthoDB" id="10599448at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="10852" checksum="ED7A2A2F13834742" modified="2019-11-13" version="1">MSGSDLGKAIPEPGGPDRNPSFAASPAIGGPDIAGAKRPSLATASRLATGSMERTSRETPLLAGSNDHLFFGRPTLSYEFYPNSQVFGRLMSCIISDTHARRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3Q1</accession>
    <name>A0A5B0M3Q1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_010958</name>
        <name evidence="9" type="ORF">PGTUg99_033306</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF152">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="148"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="47"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="176"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="691"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="801"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="924"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="969"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1186"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="705"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="815"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
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            <begin position="838"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="860"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1048"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1092"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1186"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1227"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1251"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1279"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1298"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1323"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1350"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1360"/>
            <end position="1371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1070394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1090000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1391" mass="153664" checksum="A461B0BD53458DB0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M4C5</accession>
    <name>A0A5B0M4C5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M4C5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="7867" checksum="4B050151BD687E93" modified="2019-11-13" version="1">MSISPTTIGANQPMTQRRQAVPDVCCLLPQARHGLGEELVNLNIPQPKQSEEEVESDHVQPRTPGGLIHSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5B4</accession>
    <name>A0A5B0M5B4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_017134</name>
        <name evidence="9" type="ORF">PGTUg99_024090</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000174">
        <property type="protein sequence ID" value="KAA1125948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5B4"/>
    <dbReference type="OrthoDB" id="2506628at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="7">
        <location>
            <begin position="108"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1071711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1125948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="177" mass="19761" checksum="724E168D669BE9BD" modified="2019-11-13" version="1">MFDPMIVVAKKYLALALECSPILLATILHPAWRLSLIRDKFVAHSAVTEELLRTTFKAKSKAHKKMMPQVTTPESNAESNEDEFNYYPEKSGASQEDNELKKYIEGQWPLSKKADPLQWWKLHALEFPRLALIARDVLACAGSSATVERTFSAAADVCQPGRSLAAPTIEQCVSSHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M694</accession>
    <name>A0A5B0M694_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027023</name>
        <name evidence="2" type="ORF">PGTUg99_008354</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M694"/>
    <dbReference type="OrthoDB" id="10517969at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1135178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="91" mass="10268" checksum="D8CF1F8AA71BDC22" modified="2019-11-13" version="1">MVNAEGEPVEYVAERQNAAVHDTYGMTMWSWRQTPRRPGGFPPGRRGRTALPTRRVSSWSTRLYNLADQEDSLLGRQGCTADQEGFILVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6G4</accession>
    <name>A0A5B0M6G4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028628</name>
        <name evidence="4" type="ORF">PGTUg99_021411</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6G4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137156" evidence="2">
        <location>
            <begin position="28"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="928" mass="106572" checksum="89C9D125EC170E26" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6R1</accession>
    <name>A0A5B0M6R1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6R1"/>
    <dbReference type="OrthoDB" id="2507256at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="514" mass="56853" checksum="2714554EDD7D4720" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9N3</accession>
    <name>A0A5B0M9N3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_035028</name>
        <name evidence="4" type="ORF">PGTUg99_022614</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1073036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9N3"/>
    <dbReference type="OrthoDB" id="441444at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00677">
        <property type="entry name" value="S15_NS1_EPRS_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_uS15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_uS15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15, BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321:SF26">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1073036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1084743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="39884" checksum="92E4168FCE28DD3A" modified="2019-11-13" version="1">MTRLIWSGFKTGLGSIKNSNQTTQLSITSRLGSLGSTGNKDHVGGVGKTECFSTTGAVMIKTKKQKKEIERQRLKAAEAKATLRTSLKLRQKPDPILGYSTQIPKAHLNWENCELNKLIVKKEDVWAGKIGPVELDDPLQPKSPIDRNNHQDKEGGASLVLNFGLNENEHQKAILFDGLPKMKELIKIKNKPIFDPSLTEPLPADSDSIINEQDEEDLKKLRLMRILDLRNSNSKGIDKFIKLRILKAFSPKDHLHPEKLDPGCSEVQAAMITYRIHAVLDHAWQNRRDKDSKRHLSDLVMKRMKILKYLKRTNPKSYFELLPRIGLEPRYLKDELIVRAKLPLRPGESLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBN3</accession>
    <name>A0A5B0MBN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBN3"/>
    <dbReference type="OrthoDB" id="2500100at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="373" mass="43249" checksum="C8CD2CC6CB4FABCD" modified="2019-11-13" version="1">MKPGQKVLAGSDTYCTEDCSFNPNEVFFSWLNTRLCRSRSRWRQESFCRSLIRFTHLHNTAVKKRLTQVTMNSKPFKGFSSKSFNPLNLRSFIKALPSKKVKSSNGSLKSESLSTIDFSSCAIENQVEDQVLHKRDQPISQGHGHDAPPQSVTLMTFSKVVGIFEELGPIQEIPKPRSRTRRNSFLKLKTKYSVEKTKKTSRPNTAVEAIISSGLFQPVPETVSPEKRQAQSLDHLVNSRKELLIKKETAQPLKTPSARETRLKYLLYNPPLDSFELRRRLNRKDGDEKIFLPRLDPSSYALIMKKEEHRRRMEDLVYYKNSHLHKYNQIINNGVRSLKLESIPESVGSWVVSKDPRDRRQRRRKLDYVIKSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBV0</accession>
    <name>A0A5B0MBV0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBV0"/>
    <dbReference type="OrthoDB" id="2503722at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="283" mass="33176" checksum="F33D9C6AEB115DA6" modified="2019-11-13" version="1">MLKDQNPHESGKCFTEDFFRAQWKRQRDFEINRNQTDRLKKEEQAQFFERGEALKSLAESFMASLASSSPTSDPTHALSMLQEIEDLQKKQNEEIQRLGSHFAVDEEAERNPEQEKRLALLWSAKSALYKYAVQIQGEMQPLRDSKSHGERLGTVLKEKIFEALGRRKNTVTRVIKTFCDRRTDYLKNHAPDQLSLPENKAITYNEFTELKLDDPFWNDAYLCLSKDPWAVDPTVRTGIHALLRLDRANEEMIQLRNELRRCLAWGIHYRKQLKLRIDQCVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEW9</accession>
    <name>A0A5B0MEW9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014869</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEW9"/>
    <dbReference type="OrthoDB" id="2496743at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="347"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="679"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="836"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="861" mass="98597" checksum="CAB1A48D8BFECAA4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MG66</accession>
    <name>A0A5B0MG66_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MG66"/>
    <dbReference type="OrthoDB" id="2552042at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23164" checksum="5586E24C7544FF85" modified="2019-11-13" version="1">MGDIRDFYKQERTCCLSIQLFQAGMMIILYFNTFLAIFCACMSFFGPKILALVQPVAISYIFGVLYLIVAGLHIFGIICVERNFVAHFRIFKNLTLYAIGTILLLGLMFIIRTAAQHTASVAQCMQVYHFPVDAMYKKNRNEKGGSSSAVNAAMTVCDVFSWVQVVVMAFVWVALTSVQLYFALKHRVYFELKHKDYLEDTAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGB7</accession>
    <name>A0A5B0MGB7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGB7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="272" mass="30406" checksum="A09E7962E7C8DF94" modified="2019-11-13" version="1">MSSIFDNCDTNDFYCEGRSDPSIDPDNNADGDKAPIDAVGDKTPIDVKPTMDAKDKGPAEATVDPASINTILPSELACSASLVYIPKGMEIVETRTHYFPLKDKWHAINDPSRHYDCVPIPRLFPKPHPFVCVPWNVHDPELPPGLPVPFLRTLNLPVPVPTPAPSTSSSESSSNDSDSSSTYPLISNYINLNHQGVQKLLLDDNTPPTAVCIYTEEDVMRIFEGIAPEYVDMCSEYPPSSLAESDRRVMFFENMQWFFRNKRSTYLSTRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKP2</accession>
    <name>A0A5B0MKP2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014591</name>
        <name evidence="2" type="ORF">PGTUg99_024405</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKP2"/>
    <dbReference type="OrthoDB" id="2881727at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1126818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="393" mass="44519" checksum="BA529C49D55927B3" modified="2019-11-13" version="1">MTAVNYYQLPGDQRDKWYCHLCGGGGRGSKFANLGKHEKTPSHQAAILSREEKKRGVPVPHAPSADTVWTAANDRLEEPEVECSISDHSLAADLAFIDEDSIFSSSTEGPLSNHSSYSYSSGEEVGWDGLLSGDHPHEMNLPQQLSVVQPQTRQERRNFEWWPFKSKEFLVSWMVIGHTRSIIYRATFDHVRMMFNICDITLPDWTTIRRSKEKLRKMLGLQVLSGRLVLNTPVHTLSLKRILALEISNPVVQPHIQYYPEMSEGQNISRLTQSAKWLKELGPHTRAQMVRQGSHDYYLHELVQLQNTLIVVPTFLFESGGEMYARCVTPIVNVDYTSGKLQFIVPKMLPFTSPDLTSIPVSDFTSEYTIMEAPGGTSMLEQCDNKLFGMQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKU3</accession>
    <name>A0A5B0MKU3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Beta-1,3-glucuronyltransferase 2 (Glucuronosyltransferase S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">B3GAT2_4</name>
        <name evidence="15" type="synonym">B3GAT2_2</name>
        <name evidence="14" type="ORF">PGT21_006945</name>
        <name evidence="15" type="ORF">PGTUg99_013827</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKU3"/>
    <dbReference type="OrthoDB" id="675023at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF65">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="11">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1076430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1135531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="455" mass="51119" checksum="82DDD4606D695733" modified="2019-11-13" version="1">MTRPGSSEGKESPNAISMDDYNHPSPIQNSIHEPLLLDSSGHPHNASRAESFARFHTLSSPTTPAKYLLNKPAKSRWFILVLLFLIISLICGIIIAILRSYQLQGSPTGFYLAEDRVLEASKFPERLRTSKTICSYCDCKANRTSPPLLQDSSYTTPTGTRASMLAHVWLSIRDIYCQHAWLGEGPALDLLLQTELGADIPLLIQWSLELLKPGRPTAYLYTKTSWAGRTKHLRYAYFKRHIQTIRTHLELMKTRAWPEGPGHQRWGQERQLLWIVIEDGEKLANDVAQELAASGLPYIYFTYGPTHHFGNAQQNAAYALIHRLADPTQDGIFGHGPIISIDDDSNFLPELLVIIWDVQRIGVWPMGNLGPNGWEGPVYDPSNHTLLRWEAGAVQDRKFPVDNGAFAFASELLGSTIMGPRYWPTDISGGENEFIGLIVDKKEDIEPLCYNCQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML75</accession>
    <name>A0A5B0ML75_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1077008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML75"/>
    <dbReference type="OrthoDB" id="2564822at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34863">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34863:SF1">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="240" mass="27428" checksum="2C8A07A5593DB7E7" modified="2019-11-13" version="1">MVASGITQVRLNAFGMKAKLEDVEHNYGNESANDGAYNWFDIGVFHKTKGIPLFKPINKSADTELYPMLLDLGEGYGTVSFKDIEEEEIEKIKVYSCLVHALKSTSAKHLSYGPTNTRTWHTQLDSIEKKATKLEANDYTRIEGFRVELTIRCKTLDQALQIAENSGYLDFAMERDYVYNSELPLDQLTIHKEDYFDNLFDLVDLAREKLNVRKRHDQNVPSATPRARRALGVAPVTQFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMY5</accession>
    <name>A0A5B0MMY5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMY5"/>
    <dbReference type="OrthoDB" id="10277197at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022958779" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16196" checksum="B1AE6C74964D4C8C" modified="2019-11-13" version="1">MMINIILLVGMVAFLSMTQSNAYNCDNAFQRIPNTPNSVACQNGLEARKCELDQCITYGPYGPKPLSSWVFSGCVRAPTGRVQYRVQVKEFTVNTPLKRIEILGHKFWPKTNVLSPYLTPYHCPMSHENLRRATCPENKCDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MN55</accession>
    <name>A0A5B0MN55_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MN55"/>
    <dbReference type="OrthoDB" id="10624668at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022693751" evidence="2">
        <location>
            <begin position="22"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="143" mass="13961" checksum="ACB286F4F9DF8944" modified="2019-11-13" version="1">MASIRFVSFLLALSLASGAWGFKCNTAGKTNGVCFPANTGTGTNPGTNPGTNPGGTTGGTTGSATPAQQGNKGNLSCANRTDKQFCCAQAAAAITGDPAQNTGCAPAVAGKRRKAGSFLQKGGQAKAKAKAAKAQAAQAAQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNC6</accession>
    <name>A0A5B0MNC6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SNF1-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SIP5_2</name>
        <name evidence="3" type="ORF">PGT21_009895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNC6"/>
    <dbReference type="OrthoDB" id="21471at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24139">
        <property type="entry name" value="SIP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039301">
        <property type="entry name" value="Sip5/DA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315:SF1">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="449"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="527"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="712"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="21"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="465"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="551"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="596"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="646"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="712"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="769" mass="82045" checksum="E64EB04E4F85C039" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSX6</accession>
    <name>A0A5B0MSX6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSX6"/>
    <dbReference type="OrthoDB" id="10279368at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="679" mass="76649" checksum="6A9AF4EA714E85E3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MW52</accession>
    <name>A0A5B0MW52_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011565</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MW52"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="136" mass="14933" checksum="8E9DDA36C9D7395D" modified="2019-11-13" version="1">MGQPNIILTHPTQVRDEQTNDVGRLSPPEMIVSLTSSQATVPHTQSANEGLFEGASQRCRLVKPLSNPTAGFDASLLSKDQPTAGLYAKPMLEDMPTMASNTSRYETLVDQHLWCITGTIRETWTSDNEAVKIFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXP0</accession>
    <name>A0A5B0MXP0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXP0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="490" mass="53036" checksum="45D02F580F0CCD7D" modified="2019-11-13" version="1">MQRGHHLRSSNQTSDRYEARIRHLEDVVCLLLARADLDQISLGSSTPLIGSFRYSIDQGLVPVLSKRTLKALANDWLGVKPKRPVSPIKSNCCPMSTYRSPPSPQTQSLPTPSVASLADLTVSSDPLSSSRTVTAHQNKIATIVEPHESDWLLNSSTARASFTTNEATTPTTSRTSEDPKASTFKPPQHRQTRLLSDSAIAYGRLESPKAKNTHAIISAADDSLLTLFFPNPRTVPNSDASSAIILLKTPPLRSRSLDCFLSSDRPMSDDINRGLTKLKIQPSTGDPQSSCSTMNSSPPSENLDLKADNGLTLFLSKDNLTSATNHRTDTPAHSPVNAIIIVADDLLPTSFLANPAATSATDNTSNLTPPLVSTSPPDPTATTPLLTTIPVSTLPPEPAMPNCSSDSFDIAAIGSITVMEDSTAFKDPQLWAPSLSQAALENYKDIDDYLKTLEADETEMKKKKKKKKKKKTPDPTSIPDNPVLFYEGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZD2</accession>
    <name>A0A5B0MZD2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial distribution and morphology protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mitochondrial inheritance component MDM12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MDM12_4</name>
        <name evidence="9" type="synonym">MDM12</name>
        <name evidence="12" type="ORF">PGT21_030914</name>
        <name evidence="13" type="ORF">PGTUg99_032143</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. MDM12 is required for the interaction of the ER-resident membrane protein MMM1 and the outer mitochondrial membrane-resident beta-barrel protein MDM10. The MDM12-MMM1 subcomplex functions in the major beta-barrel assembly pathway that is responsible for biogenesis of all mitochondrial outer membrane beta-barrel proteins, and acts in a late step after the SAM complex. The MDM10-MDM12-MMM1 subcomplex further acts in the TOM40-specific pathway after the action of the MDM12-MMM1 complex. Essential for establishing and maintaining the structure of mitochondria and maintenance of mtDNA nucleoids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. A MMM1 homodimer associates with one molecule of MDM12 on each side in a pairwise head-to-tail manner, and the SMP-LTD domains of MMM1 and MDM12 generate a continuous hydrophobic tunnel for phospholipid trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion outer membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="9">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MDM12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZD2"/>
    <dbReference type="OrthoDB" id="3356905at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21672">
        <property type="entry name" value="SMP_Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03104">
        <property type="entry name" value="Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26544">
        <property type="entry name" value="Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="29"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1081179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1131341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="268" mass="29735" checksum="283E7D292DEBE20D" modified="2019-11-13" version="1">MPLNLEAVDEKIAGRTCTGEGEEEHSEQMSIEIDWLSLTPSIVEKLTERLNGLLANLSAPDFLGPLRIQSIDFGDIAPEVEIVDIRDASRGFRIADGESHPSSSPECSSAPTIKPDEDGHSQLSLQIHLRLTYDGNLRLRFSTALVLNHPAAEFMSLPLRATVVGVGFDAEIILAIPGDRSKFHLSLLEPPHSELPLYEDSVENGGKRNSSIGARILPHLFVETEVGNADRHVLRNVGKVERFLTETTWTLICEEVLYPTFQTFEWNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2S5</accession>
    <name>A0A5B0N2S5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005486</name>
        <name evidence="2" type="ORF">PGTUg99_031231</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000439">
        <property type="protein sequence ID" value="KAA1082279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2S5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="138" mass="15144" checksum="681A8279677CFF2B" modified="2019-11-13" version="1">MSRSFSRVLHRYPPALYVKISSFSNYQSIHQVMSIFKTGLVILAFACLLSVVEGDCAFRCFDGVSNTPIGMCVTMGPDPKTGKYISHLKNTHNRTLMSFSATDPLIVGAVHTISFLKVVTKKGNNKDNTGGRNNTPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9C0</accession>
    <name>A0A5B0N9C0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003375</name>
        <name evidence="2" type="ORF">PGTUg99_019036</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000145">
        <property type="protein sequence ID" value="KAA1128072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N9C0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1128072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="215" mass="23659" checksum="501234BC887298D1" modified="2019-11-13" version="1">MHINVRLTKDCVQYVSNLVLAPGAQQITGSQLRGKSYDFTASRSEHVCGLATALNYLPLRSPTGHPGFRVQVCSSGMDAEGHNACLTRDRPPESPTGPHVAKLNKQFEARRDRAVEPDNIQSCRLLPENGDQKNACRRILSSLDPPSLGRTSYLALNFFGIELAPVFNSFHCNARARGLDCSYVSEPVIAGLVAQKLDAPASFVFDFNRQVKRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NCC9</accession>
    <name>A0A5B0NCC9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NCC9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="66" mass="6958" checksum="B6CC6DC0670CCC69" modified="2019-11-13" version="1">MFFCLSALISGRGGQSSHPQEDSTLHEVMLTTHSTPVGTTSRGASQTNTQTPPPGLGTRAIDHLHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NCJ1</accession>
    <name>A0A5B0NCJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NCJ1"/>
    <dbReference type="OrthoDB" id="2510036at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022791829" evidence="1">
        <location>
            <begin position="25"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="154" mass="17023" checksum="E39087F9FDFC4116" modified="2019-11-13" version="1">MVSSRFISHFSLLIVLALAAIATGAAIPARESRSDDLSGCSDGFEDIYDNVEDYDEPPVDATLVTISVTKFVSVEPLMSPFEPISPEEDFDEEMTPTIIEVSVIELTEIPEEENVSQRFDSDAEAEVDDESRLLDCDHEYLPHEFNTAPGSLYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NE16</accession>
    <name>A0A5B0NE16_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Uroporphyrinogen decarboxylase</fullName>
            <ecNumber evidence="5 14">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">HEM12_1</name>
        <name evidence="17" type="ORF">PGT21_012316</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the sequential decarboxylation of the four acetate side chains of uroporphyrinogen to form coproporphyrinogen and participates in the fifth step in the heme biosynthetic pathway. Isomer I or isomer III of uroporphyrinogen may serve as substrate, but only coproporphyrinogen III can ultimately be converted to heme. In vitro also decarboxylates pentacarboxylate porphyrinogen I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>uroporphyrinogen I + 4 H(+) = coproporphyrinogen I + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:31239"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62626"/>
            <dbReference type="ChEBI" id="CHEBI:62631"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:31240"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>uroporphyrinogen III + 4 H(+) = coproporphyrinogen III + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19866"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="5 14"/>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NE16"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000008">
        <property type="entry name" value="Uroporphyrinogen decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091">
        <property type="entry name" value="METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091:SF169">
        <property type="entry name" value="UROPORPHYRINOGEN DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="9 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="16">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1086804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="387" mass="43208" checksum="6A50573AA1E660BA" modified="2019-11-13" version="1">MSKETVTNNSPTNDLLLRAARGEKTERSPVWVMRQAGRYLPEFRKLRESHGFFEICRTPELATEVTMQPIKRYEGLLDGAIIFSDILVIPQALEMEVIMNPGPIFPNPISSKEQMEEMINKKISPEELKEKLNYVYEAIKLTTLELQNRIPLFGFVGAPWTLFAYMTSGGGGSGLNASKATSFDTAKNWIQVHPELSARLLEKLALVCALHLVNQIEAGCQIVQVFDSWAGELSDYDFEHFSLPYLRRVSTLVKEGLSQANIHPVPMVVFAKGANLPHQLQQLASSGYSTVSLDWKVRPAYAIEALESLSNSIALQGNLDPAVLHTDHQTIKKYVRLMFANQEAGGFLPHLPHITNLGHGITPGVDPEAMRTFLEAVHEESTKIRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEF4</accession>
    <name>A0A5B0NEF4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEF4"/>
    <dbReference type="OrthoDB" id="10276735at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1087133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="410" mass="46635" checksum="B9EFF8402DFD620F" modified="2019-11-13" version="1">MAPKRKTSAGKKHRKQGDRVVEGFERLIKKFDPTAAGNILRSHVHEPFQIDQSEVKRDLLHDLRSNLLPQLGGQIINLLLLLDPNRLREDPGGHLKLILGIQSELDQTLSRIESATFSYPHATEPLSDSIEFKDQDLKEIKWFRLDGLYWRINGEFLVVVTRIFLESNELLQQMKLSTTEFHDQPDIDYTREQIVYYASSSLEGIELAIEWLDGSEFDLVQIDWPSENDGFSENMDDLLSLTNRTSHLEQGIITGGSAPGDDPAIKLSKSLLPVFKLCRLLFKKLSKRGLNKEHLPLFTKASSVELEHVADMGSVVSYGLRNFLGVLESVDRSRNRLAHPDCAIMAAKIQDHVEIDVSLITHYFVPLIPDTDPLPIQNHYKDWLATWTSQFRLAIRNFLNAALSFDEGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEH2</accession>
    <name>A0A5B0NEH2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEH2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="88" mass="9311" checksum="988B998691DEE067" modified="2019-11-13" version="1">MEDDHEAQFEAKSISKGVVVKIAGHPGGPVSSVGRAFGFYNLLSGSDTEMSGTAEDYTRPSEGVSMGSSHSDAQSSYRPIMKPGAWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI74</accession>
    <name>A0A5B0NI74_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ML-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_005154</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI74"/>
    <dbReference type="OrthoDB" id="2115177at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF2">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022700930" description="ML-like domain-containing protein" evidence="8">
        <location>
            <begin position="34"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="472"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="574"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="606"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="9">
        <location>
            <begin position="36"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1089051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="664" mass="72526" checksum="72A2D6F2787BA2E1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJW8</accession>
    <name>A0A5B0NJW8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_001580</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJW8"/>
    <dbReference type="OrthoDB" id="341259at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
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        </location>
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            <begin position="168"/>
            <end position="200"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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            <begin position="222"/>
            <end position="338"/>
        </location>
    </feature>
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            <begin position="365"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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        </location>
    </feature>
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            <begin position="1"/>
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            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="415"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
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        <location>
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        </location>
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            <end position="573"/>
        </location>
    </feature>
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        <location>
            <begin position="576"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1088946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="606" mass="64434" checksum="B877D3408C272DF7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NL51</accession>
    <name>A0A5B0NL51_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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            <end position="30"/>
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            <end position="170"/>
        </location>
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            <begin position="1"/>
            <end position="21"/>
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            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="207" mass="23302" checksum="74C07245B22E5506" modified="2019-11-13" version="1">MSSSGSSSLGSLLLQGSSSDSCQTQNGVNPLSSLTKTFERQPYASEHRFQPTTIKKNNAARTTTNELHQQLTQEQQAIYHHQDHHKNWSTAFLRSVPIQSFLHSSSINNPQWQSEFNQSQSNQPQRNLEHQELNQPRNIVPSTAHLGPSLGFSQPGRLTPHHHPYHPIQPTTHLINQEPAIQHSNSTTAPGKYLLTFEISKKIIAMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0NV90_PUCGR</name>
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        </lineage>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
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                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
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                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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            <begin position="1"/>
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        </location>
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            <begin position="22"/>
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        </location>
    </feature>
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        <location>
            <begin position="170"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="787" mass="90668" checksum="4D160FD7138A95A2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZT4</accession>
    <name>A0A5B0NZT4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="63"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="127" mass="13747" checksum="894972A0D78E6D64" modified="2019-11-13" version="1">MTETMAAEQTGTSIHLGIIRQVRNLARDSGSIIGAGPSGVFSALLRLPSVCFCVNLATLTKGSESSRDPLERTGFARRAGKRLGAGKRSCQLEHHKNDDLSHGKPHRTCLAQKLKENALRVLSRLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P062</accession>
    <name>A0A5B0P062_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">UBC4_2</name>
        <name evidence="9" type="ORF">PGT21_033534</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P062"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000010">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2-16 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1094957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="145" mass="16213" checksum="12982D9DF7D44B95" modified="2019-11-13" version="1">MALKRINKELHDLGRDPPSSCSAGPKGDNMFQWQATIMGPGDSPYAGGVFFLMITFPTDYPFKPPKVCFKTKIYHPNINSNGSICLDILREQWSPALTISKVLLSICSMLTDPNPDDPLVPEIANLYKTDRAKYEATAREHTRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P337</accession>
    <name>A0A5B0P337_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024883</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P337"/>
    <dbReference type="OrthoDB" id="2505969at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="55601" checksum="B765530ACE569E20" modified="2019-11-13" version="1">MWKHSAVPISPFSRAVDEFLDAHNPLILVPSNNNESDYAFTPRQWRRTLSSAVDAFREMLRREELLSESLLSMNPIDKMADICPPCYGPQVPGTRADEPNYIVCMDGNFQHRRHEAASVKVPGMLKTPSLFIKPDEVAQMELSMNPALHNAIEGDVNRHLQQLPDTLVMLEGEIESILDELGTDYFQNLQGGSEAEIKALIKIKISKSKLYEAKVGVIEMQKRWDQPRSGTRVQHPFKKQMNSKMNMFKKKWISYNNRATSFNSDFSPEVPLETPSFDDVKAFGIDNFFWSTSRLDHPSEPWAVDVNTQKGIQAYLTVTHCQDELRRIAREARQTVTWAIEKSIKMDQLLQSLQAEPQETDVLTESQQQVKNTCSEFNCPKAVVQSVFTNIAKKHCRLWLSWNCHIWKLLRWSKNYLDYHQETEETPEYQWDLIINKCRQTWERLVSGQSVVMEFDSEEDQHEEEIFAQEQDFIIHEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P862</accession>
    <name>A0A5B0P862_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P862"/>
    <dbReference type="OrthoDB" id="2501845at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="441" mass="50067" checksum="3FF90999AC47D338" modified="2019-11-13" version="1">MYTSKLAFSLGQQSHRSSISLAFEGLPYSQSVDSLSKDPMKCSRSTSAILTYFYSLSVLSYVKGSLGGLPKLHLTTYSLPEPPQQSKSITSGTGYQLEYPIPKAVARSKDTASKSTHKSSNSVHKNQEGRTLCCFGSVHARVNSQEKGSASVPKRVSREKLEKTLIIEHPVVDPSSNQLQADQMEKLKQWKSKAEVAQFMEKLELGITEIVNSLIYLEMQRFSSEDLERLLARFTENREFVRTPATFAFLVHTFIIDVYRSRVGSNQPNLVANAYLKNEYGIYKRYAREQIFMLAKIEIMECLEGMISKVSRYYEKAKLEIDTWKEQVDSVIEMLKLIEWEMSPFHSQEIQRTLLRNQLLQTKARSGDRGLFEFLETGAQMNVSVESLQSFLLLNTKGTTPEHREKLFLHVQSIHDDLSEICDGLDPPKINHRDALRGAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBF9</accession>
    <name>A0A5B0PBF9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBF9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="52" mass="6160" checksum="72F5E9A71B6EA480" modified="2019-11-13" version="1">MYLFFPLRFTSSISSSQKSTTLNSYRKKDIKVSLYNSHVYQSEKFIFRDGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBM7</accession>
    <name>A0A5B0PBM7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026707</name>
        <name evidence="3" type="ORF">PGTUg99_034093</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBM7"/>
    <dbReference type="OrthoDB" id="2495772at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137732" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="16609" checksum="FCBBFA86AFF98672" modified="2019-11-13" version="1">MRSTISLTISILVALIGQAFSAATVTTDNDGPKQCMTYTGANTDTATCNDIPDMICTKGCRDSFVVAQGCLPNDGSAAPTDLPGTQVCTVGFGRDTAAAKACLTESKGFSCKGDTTGYTYCYGCAKTRITYKVPPSSETYPPSWLNQSLTHPALPPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PC19</accession>
    <name>A0A5B0PC19_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">14-3-3 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">BMH1_1</name>
        <name evidence="6" type="synonym">BMH1_2</name>
        <name evidence="8" type="ORF">PGT21_037049</name>
        <name evidence="7" type="ORF">PGTUg99_001608</name>
        <name evidence="6" type="ORF">PGTUg99_006083</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 14-3-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000421">
        <property type="protein sequence ID" value="KAA1084993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000342">
        <property type="protein sequence ID" value="KAA1099147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PC19"/>
    <dbReference type="OMA" id="KGCQLAR"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000002">
        <property type="entry name" value="14-3-3 protein epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="4"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="234"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1084993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1099147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1120041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="264" mass="29345" checksum="62E97DCD35CF9944" modified="2019-11-13" version="1">MASREDSVYMAKLAEQAERYDEMVTYMKDVAKLDQELTIEERNLLSVAYKNVIGARRASWRIVSSIEQKEEAKGNEAQIGQIKAYRDKIEAELGKICEDILEVLDSHLIKSAESGESKVFYHKMKGDYHRYLAEFATGEKRTASSDASLEAYKAASSIATVELAPTHPIRLGLALNFSVFYYEILNAPDKACQLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDLNDSVPDDKEEKAAEPAAAAEGAAEPAVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCD4</accession>
    <name>A0A5B0PCD4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCD4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="123" mass="13768" checksum="BD5FCBD8A97FB684" modified="2019-11-13" version="1">MELKLHPLNPSVDHSCSLHRSGLSALPNNPSDNPFNHFKILDNEQLPEERDQTLLSSGKDKIDSLQDSSEGYFRPPDEASHHDLSKGGSAAIYQIQIFHLLPLLYYFTPAEMVGVGTCCYEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCU3</accession>
    <name>A0A5B0PCU3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006629</name>
        <name evidence="1" type="ORF">PGT21_006953</name>
        <name evidence="3" type="ORF">PGTUg99_010885</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000210">
        <property type="protein sequence ID" value="KAA1123220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCU3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="112" mass="13220" checksum="A27B82F013F9A773" modified="2019-11-13" version="1">MRDSNFSIVGRTKSRPATKTKTAFIQPIHNRLALEIDWQSRSKPYNTFLWASANTFNFWSHGHTDLCKKRACHNVFFYWRYNFKIIVIHFLAEVRSPPIACELVEEMSLILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGX2</accession>
    <name>A0A5B0PGX2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGX2"/>
    <dbReference type="OrthoDB" id="10285508at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="346" mass="37440" checksum="E7F2ECB86004ED23" modified="2019-11-13" version="1">MENSQNHLSAAATPIPKIVSSVLQEASTVDVTSHPATINNITTNRIGSYQNLSILDAFFHPNDSQNKEGSAFPQTRLIAVAAAVISVSFFVAVCIWACFRLRNQHRAEALSLPHTSRPTTHIDLSSPEKSVPSTKADFIGSPSSGFGTNTPTTSTFCEEFDFVPAVVPVIHSPTTIMHHGDISPCTKGAYGSPLIRAGSQYRIPSRPDYLNFVPRVLLTGNLLPPQAVAVSDSKLLHFNVSHGVAMKTLPEPAPCKMSPAEASTELEKLIQQVNLRIFEDFDADDSFTESDSSSIPEGLSGLTGDSLPFGGAQRLMQPQLLLPLPLPLPRYNYRKLTPLRQSPSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PH25</accession>
    <name>A0A5B0PH25_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PH25"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="88" mass="9679" checksum="F3DCFA6CFBE54CA6" modified="2019-11-13" version="1">MATFQSSNQEQIDRQLVSASAKNTSSMSSPQNQALYRVHANLSGASSDVGRQHRNSQMNLKSNKRGCSNRSRDSKSDAMEFGIIKSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIB7</accession>
    <name>A0A5B0PIB7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat-containing protein-mitochondrial domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_018227</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
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    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF23276"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
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    </reference>
    <comment type="caution">
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    </comment>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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                <person name="Steffenson B.J."/>
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                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
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                <person name="Nguyen-Phuc H."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="EMBL" id="KAA1101509.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PPW5</accession>
    <name>A0A5B0PPW5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">PGT21_034984</name>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Mis14/Nsl1"/>
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        <property type="entry name" value="Mis14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="258" mass="28994" checksum="3C5392E9CB5328B9" modified="2019-11-13" version="1">MSTSEPMPGGSDCLIKFGSRADYNRFVGQLRSQLRSYLISSCQENNIEIGSPDYLAVESVLMDVFMKYTLEFIRENLLVDGRPLTPPPEFSEPSRRVNGTGSADQPNSHHNRSNSSRSKEPKPPRTRPCDTALNERVIRQQYALYDAAVENTRKRKDLPGHIVQRIEAAAEQSTKHLHSQTKEVDQLEATRAEEAANDPRQRWDVLNAEPPNIQETQQDFTGANQAICKLIQSLPQLIENASRAINLHDEVANALKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PR99</accession>
    <name>A0A5B0PR99_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamate synthase [NADH]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GLT1_5</name>
        <name evidence="3" type="ORF">PGT21_006689</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PR99"/>
    <dbReference type="OrthoDB" id="4327079at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015930">
        <property type="term" value="F:glutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051394">
        <property type="entry name" value="Glutamate_Synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate synthase" evidence="2">
        <location>
            <begin position="59"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="323" mass="34518" checksum="7F42180511F6C955" modified="2019-11-13" version="1">MLFVQPPGLPESGEYHWCDGGEAHINNPVSIANLQDAVRSKNQSAYDVYSQNAQKQVRAVTLCSISQEAHSALAIAMNRLGGKSNTGKGGEDGSRSLIMPNGDTMRSAIKQVSSGWFGVTSNYLPNSDELQIKMAQAAKPGEGGELPGHKVSELIAKTRHLTAGVGLISPPPHHDIYSIKDLKQLIYNLKCANPRARVSVKLVSEVGVGIVASGVAKAKADHILISGHDGGTGASRWTGIKYAGLPWELGLAKTHQTLVLNNLRGQVCLQTDGQIRTGRDGQISADTCQRQQMRMSNSAEMVSGYPNTNGYLDAPRPSLVQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRP9</accession>
    <name>A0A5B0PRP9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">USP7_16</name>
        <name evidence="11" type="synonym">USP7_43</name>
        <name evidence="12" type="ORF">PGT21_021728</name>
        <name evidence="11" type="ORF">PGTUg99_030371</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="11 14">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRP9"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000128">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024729">
        <property type="entry name" value="USP7_ICP0-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF644">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12436">
        <property type="entry name" value="USP7_ICP0_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MATH" evidence="9">
        <location>
            <begin position="26"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="184"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1164"/>
            <end position="1189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1068536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1104395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1189" mass="135512" checksum="F2EC39C29FFA1F25" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSX9</accession>
    <name>A0A5B0PSX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011849</name>
        <name evidence="3" type="ORF">PGTUg99_002987</name>
        <name evidence="2" type="ORF">PGTUg99_008800</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000483">
        <property type="protein sequence ID" value="KAA1070896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000317">
        <property type="protein sequence ID" value="KAA1103740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSX9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="78" mass="9072" checksum="FD52E26245528D6E" modified="2019-11-13" version="1">MMEIEPPQVKDTPHQRAVINVQNAARVQEIQAAISKRTQELHILPELVPNVLGIWNMQRMKYGRDSTQVYHHQQSTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PU49</accession>
    <name>A0A5B0PU49_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PU49"/>
    <dbReference type="OrthoDB" id="2506685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="428" mass="48446" checksum="A6411BC2041A6D2D" modified="2019-11-13" version="1">MSLPYTVPDLEDGFAPTTPLGNRPFASGSNLNWNTGFTAPPPPMKKDKGKMPANQYREPQVEEEMSDSEDPTPQRQAKQTEPPVTGYGLHNARGSDGEPTERRYGPRIIKEPGLVYDGTNFTQFLARYERAALAFQASDYEKVLQIGRFVHKEELKLELEAMEGYDSFNWTELRKAMKESWGELDNTILYTTADLVKIVEEYSRKGGLKDYKEYKAYLGKFTTILKYLVDNEHLNKKQDASLLFISAFSKESQKNIKRTLVSNGQLPKGPDGSSKPPLWKHVTEAADTEIRVEEEGYFAVSGFSEANKRMQRELDQKKGDGQRREQMISGAPTGKVVEKQVEDIMQEMASLKQQLKSVLPVYNQQSNPKSEFSRGNAKPSTPIYKPMIARASEEKRERLVPARWATFKTNPNRSCNSLGRPKDAGTHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUE6</accession>
    <name>A0A5B0PUE6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007109</name>
        <name evidence="3" type="ORF">PGTUg99_013814</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000315">
        <property type="protein sequence ID" value="KAA1104931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUE6"/>
    <dbReference type="OrthoDB" id="10269039at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474405" evidence="1">
        <location>
            <begin position="24"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="132" mass="15375" checksum="78EB2B327B63902D" modified="2019-11-13" version="1">MKRSPIWILALLLMLEAICKTSAAGEVHEITSWVSKENNAIDWSTMKFLRDFQPSHDIEDHYVCHVGCLHLSLSRVAGSKIKLVNKGDTSFQYAVQDMFIKRWIWEETIAPDHVHIFATTHVKFYLINHPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVC1</accession>
    <name>A0A5B0PVC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029918</name>
        <name evidence="1" type="ORF">PGTUg99_014512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000414">
        <property type="protein sequence ID" value="KAA1086077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVC1"/>
    <dbReference type="OrthoDB" id="2501377at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="172" mass="18442" checksum="32B4F780C5FE7D4D" modified="2019-11-13" version="1">MIIVKVLSSLPCSDVGITSDLARRVTISFRMCCGDALFDPADLHISFQATQAQYAGSTFLKPAGSSSPATAPLAMVYLEVIAPPADEFGTAEFKAQLLTRLKKEVDDWISSSFQFKSTSLSTGLNPSAQAEPTGLTSAVEFSDVIPVIEYRQMQYGSHALDGHIVVSPYGVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVN0</accession>
    <name>A0A5B0PVN0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033183</name>
        <name evidence="2" type="ORF">PGTUg99_021246</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1099033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVN0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="201" mass="22520" checksum="A87A7263797869E1" modified="2019-11-13" version="1">MNKRHQDLVRVNKDRNIQKSARRAALEVSNNEVTTEDVEMCDTPVCSHSRPAGSSWKFSFPHPSTNCLSGLEDIDATNQLEPVIGINDERDEVDDDGSRLDEAEAIPPGTFGEPDGESDDEEAELSWMEFVDVAVYQINSEPEDLTLDSSSPLYRRQEEEIDKLEPNNSAWYPFLNRESSPLGSLANNASEDTDPDQSHYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PWD5</accession>
    <name>A0A5B0PWD5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_032122</name>
        <name evidence="3" type="ORF">PGTUg99_012470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1098985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PWD5"/>
    <dbReference type="OrthoDB" id="10366909at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="475" mass="52489" checksum="30492692374552E9" modified="2019-11-13" version="1">MLRVESLLSSIPVGADPYDYIKDSLVKWGSVPLCRAALVSLVVILILHAFIAATCLLLLTLPCIRPLKRSPWYLRKLYIEDHSSGKAHQVPLYFANAGVLLTTSQLLSSASTSAFIWFDIGAASPGEHLFGSRALPAVGLMNLAEILAYWSLSHCFLVITICHGNKKQQEKSMASIRHIPPQLINFIFTFVPIAAMVITIFVIAWVTQVTSEIQSYTKQGLTALDQGSSIWKQLQVTPQSLEAQAQLRGITMQLASLFHNAFPRLEVAFYRFRLGECLFLSFESISCPIFVASFWILVQNYLKQSPNSSIKSCSSGGSTAHMNTGTESGTQTNTHSAVANRSYFESIKTDRQFFHLNIRALGTFISMLLNIILHIVNISRTTDTLTVPYWRTVATLLGTVGSIFSGIPVVWQCWRLHVDQSDATADCSNRYALAVVKARESHVPLDASGARGLKVPVTIHFEHSDTLVAPKPEMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZE7</accession>
    <name>A0A5B0PZE7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZE7"/>
    <dbReference type="OrthoDB" id="2507323at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="472" mass="52448" checksum="88CE2F330192F83F" modified="2019-11-13" version="1">MPCRSKKTWKNKNDDSRGKPFRFRRSPVKKKAAKPRPVNRNGRVTGSDSEGTGSRREGCKSTTDIDEISETDSAGLQDLRSSGGHQTTAPNDQKIDVERKRRALLAMTDWAGLHIPPLSDLHHQTPTSYKARLKGKAPAQMTTPERVIVTKPASEVETSSALGLDSNSSIHSDSRANHMNTFSLSPSSHDPIVSPTPTSPRIKKPASKVKVPSLSSKTGLGLNVPKTRSELENSLRIKIDVKSNPRSLYHKNESIQKAPSDQHLSALSVPSKTDRREVFRPEVPLWIGRISEALGFSPPTAISEFEKQSAIEIESQHSPPSIKLHDTTPDLELFVKPEKLAISHGRMCSLAHVSSEKDDLNGNPEPGKTPSADHEQEEKRKSTRTRKAVKKGRNQRMKQKKDLRQDESLETWLPQKRMTNYCKNDSKRQIKEIGKTGILIGGGGIWWASPRTISSIYSFKIGMMTNGSSDWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZY9</accession>
    <name>A0A5B0PZY9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="61370at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1106329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="174" mass="19001" checksum="948C5FFBE24D2416" modified="2019-11-13" version="1">MMKNRTGLATSGSRSRRSSRIVPLGGGSVVLADPPPQRNGFGFCEKWRTATYTAQLSIVIAVINLFATFLILIGKNTAGRSFALLIPHFCIWCVCVAVFQSLAWILILNVFNQDDRFYFGSRLSTSIYISIITSAHDLIVLTAIVATGFTGIFVPASSSSSSGVDRRDEYEPIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0B3</accession>
    <name>A0A5B0Q0B3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036669</name>
        <name evidence="3" type="ORF">PGTUg99_007219</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0B3"/>
    <dbReference type="OrthoDB" id="2497684at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137922" evidence="1">
        <location>
            <begin position="20"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="265" mass="29356" checksum="AD79CC391900BDC5" modified="2019-11-13" version="1">MAALVRLLAIILLFQSGWALTVVTGEGYATSAFVESYFKHTSRIFHNFAQSSRAKILPNVLGEGFTGSTRTMCTRWQSGAWSLISKTIATFQDHFNRRGKQINKDPHLHLGFRATSHWPISKDNTSTELMMKSYHTQDSELLAQIVEPSPARAVINYPAETFVVIPPNTLFSQVSSVNSLLASQSTSKTSTPFVPLVPHIMKSLSFSSTHHATKKISRDIANASINLNASSSRSDKRLVYRRPAGSPMAMSAIPLQRGRMAAWKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5Y6</accession>
    <name>A0A5B0Q5Y6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CFA20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5Y6"/>
    <dbReference type="OrthoDB" id="7486196at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040441">
        <property type="entry name" value="CFA20/CFAP20DC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12458">
        <property type="entry name" value="ORF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFA20" evidence="1">
        <location>
            <begin position="60"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="155" mass="17532" checksum="79CDDD4324228DE4" modified="2019-11-13" version="1">MYTLDPLGRRGRDQAGDRILIRASTFQTEARSYSRSKSNKSGHTKLIHLPLVFPTSEAYTQTKWNDLLLPLDSLIPTRYHSTDFIQVHASVRLRRIYFTSNGKQASSHSNRNQDGLDLESQQVITAEGTRVVSASSVFRPKLILFKWSEAQSGAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5Z6</accession>
    <name>A0A5B0Q5Z6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018832</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5Z6"/>
    <dbReference type="OrthoDB" id="2955859at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="142"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="955"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="955"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1008" mass="115267" checksum="F910326B513E1467" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q752</accession>
    <name>A0A5B0Q752_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Defect at low temperature protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DLT1</name>
        <name evidence="9" type="ORF">PGT21_032916</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Required for growth under high-pressure and low-temperature conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DLT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q752"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038869">
        <property type="entry name" value="DLT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40021">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40021:SF1">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1109086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="457" mass="49732" checksum="F98BBD713C5E2150" modified="2019-11-13" version="1">MGTPNKPSNQSISSSASSSSPSHPFPRSSSSSSSSSSSSSPQLDNPTNSLSSSSSSSSTSFLHTSSFIFFVLLSLAIACLCVYDIIRQTTSGLRSSTIQLTSIALGSCLLVLVVSFFTSISRYLTVKHTLTLIPKPYIPISQVDLPNPIFQTINQEYGRIAIITYLASTPDNPHQPPAQERVGLAGWGKPGTIHHGKRFADQILKDFKTLEHALDGLLPIQPKPSIPTRQSSTIDGPLTRYLHILNPSSPTQPTPILLTPAQKHILNRYALLIQKSLAPSSIVTESDYQVACKCLVILVDQIQLSRSPDWPNPDGYGPKGHPLYQGIRFKDAIIETAIKLEQSLAQKLLAQSVKPSKTASLTPPSTMQTSRPGSGMRTTTITGTMNGKEAIENLVKKLFPLIYPPFLPSAFGPIITGYENLIKEIAFLHPQLERSLEEYEYALRCIGSLISHVQKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7C3</accession>
    <name>A0A5B0Q7C3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DOA4_1</name>
        <name evidence="11" type="ORF">PGT21_032199</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q7C3"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Rhodanese" evidence="9">
        <location>
            <begin position="467"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="910"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="148"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="360"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="645"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="690"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="708"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="768"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="786"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1109051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1271" mass="140310" checksum="BEE81F0D37651496" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q820</accession>
    <name>A0A5B0Q820_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033227</name>
        <name evidence="3" type="ORF">PGTUg99_031202</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q820"/>
    <dbReference type="OrthoDB" id="2503796at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29104" checksum="AFE08E1A896B2016" modified="2019-11-13" version="1">MPPRTRLKTHATHDQDRPSDTPSPSDQPHNDNNKDVGRNSSEEPSSPRQSNHPLTDAEELDRAWKVAANAVSSSYNNYHVPELSDQKDKSGRFMIAYHCKMCSTKINRPMSDSSCGNLNKHAALCLRKQQEASKTRTLASVGITGTGDIDPKEVPQLCAVWCAEAARPFSALVDASHKALLHPTVLKHLPTRKAVSKDIHMLYSAIQYNYRTVLKQHQGALYLGVDVWQSPNGFDILGIVIYRLADDNPTNFKLEATVGQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9Q6</accession>
    <name>A0A5B0Q9Q6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fumarate hydratase</fullName>
            <ecNumber evidence="2">4.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FUM1_2</name>
        <name evidence="7" type="ORF">PGT21_004768</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible stereospecific interconversion of fumarate to L-malate. In mitochondrion, catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH. In cytoplasm and nucleus, involved in DNA repair in response to DNA damage: following DNA double-strand breaks (DSBs), translocates from the cytosol to the nucleus and promotes DNA repair by catalyzing the dehydration of L-malate to fumarate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9Q6"/>
    <dbReference type="OrthoDB" id="1738025at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006106">
        <property type="term" value="P:fumarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01362">
        <property type="entry name" value="Fumarase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000002">
        <property type="entry name" value="Fumarate hydratase class II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000001">
        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000001">
        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00743">
        <property type="entry name" value="FumaraseC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005677">
        <property type="entry name" value="Fum_hydII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00979">
        <property type="entry name" value="fumC_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008909">
        <property type="entry name" value="PRK12273.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444">
        <property type="entry name" value="ASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444:SF1">
        <property type="entry name" value="FUMARATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="5">
        <location>
            <begin position="82"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarase C C-terminal" evidence="6">
        <location>
            <begin position="479"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1109958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="540" mass="57883" checksum="9C111EF317C0E638" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAM5</accession>
    <name>A0A5B0QAM5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Plastocyanin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAM5"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13886">
        <property type="entry name" value="CuRO_2_MCO_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000045">
        <property type="entry name" value="Laccase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF511">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="2">
        <location>
            <begin position="47"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="277" mass="30305" checksum="1B5D83F93F2EE370" modified="2019-11-13" version="1">MSYRLQPGPFTGTTFWKAGYGTQAADGLTGGLVVHPTHSNTTVTFDEEVVVHLSDLYHENSKVLTEKYISAEGIGGKAGNEPVPDSGTINGIGQYPHRPNGEIIFPEYHKLHVEKTKSYRLRLINAGSMAPIVFSVDNHNLTVIEADGVEVEPITFIGGIQVEVGQRYSVILHAEHDHHEQYWMRAALVADGFKYDNPESNRQILGIVAYGEGLEGVPHSEAHEEKIEILDPTRLRPLVPTNPPPATQTYMVDYLLRQSPGGSTGKAFDSSFVSSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB42</accession>
    <name>A0A5B0QB42_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QB42"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="83" mass="9403" checksum="EC711B0D13E30F70" modified="2019-11-13" version="1">MVALMEPDNLEGDESTERMSVTNCSYACQIEEAQNAISQRSMERNIPYYAVPDALVIWNIQSMAGKPNTERRLQMSTFNNQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBL8</accession>
    <name>A0A5B0QBL8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024284</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBL8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="433" mass="50339" checksum="88C38138149D7B0E" modified="2019-11-13" version="1">MPFSSINLVIATSPEEALPARYWNDDAEVLNFRFEDFDFRILDSPVQTPEHKPIENGSQLFLPNQRSCNLNRLDGLDVAGNHFMSYPSEPFIESMDCFIPRIGDETIKKRTYHDTTRPENESSWEKEVRLNKKTKVDQQMHYEDPHQPLVSNEEQKDIGRAYVREDSKDEGKNIHLCKGRINRIDQFKPLGSDHFKSIIRRAAVAIHRQWPADAQAYKQFQTQIDERFENLKLVSQKYLPCKSSDVIKIEGLPIILESLKQSPKDESCHDTFIRLSHHSEDKWGRIRKKAQIQYKIQKIGEALNSLHNLVGLNGLDFKIIGKHEHMHQDLLDWFEGLIFKNTEAQFPLLGLVQLNPSSNISKASFNCAQIYLASALTDLTSINRKKAIQIAFELIRYWYQEKSSTSNNYHLSDEEYWQLISHAVMPYFTSPMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCR2</accession>
    <name>A0A5B0QCR2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCR2"/>
    <dbReference type="OrthoDB" id="2508307at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="169" mass="20018" checksum="E684E1E9D31D0A9C" modified="2019-11-13" version="1">MNIDRFEQILSTPESGQLRAEEEKFFDLYYGFSQTTSKKEKAWSASQRTGFLRQMRNEFRNQVGIWFNRWRQHTTIDVEKYIDSMTKYECQKIVPVYLFYVEMIRSIVPRPGVEREYSKELGDALRSFSRLIQLVIHRDKLNQTNLDRITINKAITLSKQLQASGGDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCW7</accession>
    <name>A0A5B0QCW7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCW7"/>
    <dbReference type="OrthoDB" id="2497259at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022896240" evidence="1">
        <location>
            <begin position="19"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="15443" checksum="349A8152D0B3EC57" modified="2019-11-13" version="1">MLVKICWIVCFLLASVAALHPKVVSASDQNDCLQSTAKTTEPEARKQHFNRGISNGSGFLFASQKTNFKNRGSKSAGMRNRIMKDLAALKMKFSDKDEVSVRWTIDVNTPDVPTASATRKIKVQLPLKIIFMKSNKLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDG4</accession>
    <name>A0A5B0QDG4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_036062</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I NDUFA5 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QDG4"/>
    <dbReference type="OrthoDB" id="286811at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006806">
        <property type="entry name" value="NDUFA5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04716">
        <property type="entry name" value="ETC_C1_NDUFA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="175"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1111054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="278" mass="30715" checksum="AD2A8C4293E27709" modified="2019-11-13" version="1">MPFSLSTAARRSRSLISASSQSSSGSHLLHHSSTLIRAKYASSVSQSPPAFEVPKTEREVADMLAKAGSNEERKRIFDALKGVQEVAAKVPPKARGPAGVHLIPIGRTKLTTNQTGVDVHHSPFESLSKTYQDTLKALEKIPASTVYRQAVHSTTRNNLEIIKKYLSAAVPGETADAVEQRVKAAENELGVEMIEKAIEQAEDEHQLVLKMIEYKASVSQIHSSYFLLPLSLSLQTLEDPLMTSPFSRFRSWQELEEKPAPGQWDYFEIPASTSYPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEH5</accession>
    <name>A0A5B0QEH5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006862</name>
        <name evidence="3" type="ORF">PGTUg99_028613</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000206">
        <property type="protein sequence ID" value="KAA1123888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEH5"/>
    <dbReference type="OrthoDB" id="2547765at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366511" description="Secreted protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17231" checksum="2CA7F2063D9E1B73" modified="2019-11-13" version="1">MNISYFNLIGCFSLLSIIQIGLVAGQSYLDQSTPLYVTSPACASYKCEVVRQPGDALHINWLNAPSGDVKLVLASETNGKSYTIAERIAGTQRDCHHGDTGHRPCGQYTKIVPANWTSGKYSVQVISLSNPDKIGYTDIVSITKSRHMARNRTRLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHA8</accession>
    <name>A0A5B0QHA8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHA8"/>
    <dbReference type="OrthoDB" id="19657at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050471">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433:SF5">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="60"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="316" mass="35327" checksum="D317E2A25D80FE27" modified="2019-11-13" version="1">MLCNVAADLPTLPTALDNESCVEKGLCPIKPTNSSHQPRHIYYEVHGDLKASQKIVLVMVQYFSKKKDHVVLVFDNRGTGNSNAGAIEAYRTSDMAQDTVTLLNWLGWNQDRSLHLFGMSLGGMIAQELCLIVPKRFKSATFISTRCGSELDWPSKGAWEVLVKTSAKKANGDEGLDLYLNVLFPKEYMETVVEQDTMLKSDLREKLRNCHRLPREQAPIPFMGHFYAATMHHCPHPKLHRIAADLKPAKMLILTGGSDELIPTKRSLELHKNLPGSELVVLENAGHALIFQLSEEVNTIMERAIVEGNQAFKLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIV8</accession>
    <name>A0A5B0QIV8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIV8"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="715"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="846"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="928"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="846"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="883"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="945"/>
            <end position="963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1184" mass="131612" checksum="6E6D4E362CB74992" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMU8</accession>
    <name>A0A5B0QMU8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione gamma-glutamylcysteinyltransferase</fullName>
            <ecNumber evidence="1">2.3.2.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_004353</name>
        <name evidence="8" type="ORF">PGTUg99_031622</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.15" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000204">
        <property type="protein sequence ID" value="KAA1124412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMU8"/>
    <dbReference type="OrthoDB" id="448954at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016756">
        <property type="term" value="F:glutathione gamma-glutamylcysteinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098849">
        <property type="term" value="P:cellular detoxification of cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010273">
        <property type="term" value="P:detoxification of copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046938">
        <property type="term" value="P:phytochelatin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.30:FF:000001">
        <property type="entry name" value="Glutathione gamma-glutamylcysteinyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.30">
        <property type="entry name" value="Phytochelatin synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040409">
        <property type="entry name" value="PCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007719">
        <property type="entry name" value="PCS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038156">
        <property type="entry name" value="PCS_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33447">
        <property type="entry name" value="GLUTATHIONE GAMMA-GLUTAMYLCYSTEINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33447:SF2">
        <property type="entry name" value="GLUTATHIONE GAMMA-GLUTAMYLCYSTEINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05023">
        <property type="entry name" value="Phytochelatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51443">
        <property type="entry name" value="PCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0104">Cadmium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Peptidase C83" evidence="6">
        <location>
            <begin position="92"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1114325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1124412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="483" mass="54159" checksum="FBF969A86D483908" modified="2019-11-13" version="1">MFHSILIRTTTKLRTKESIIFSSYSTSNNIPTDRPNLRLAEDIYHQHPISIKPTDKPQLLKDLRHNYPSASQTHSSPMSDHQSNELEAEKSNPARSFYQRQLPESCLSFTSDRGKALFRSALADGHLESFFPLASQLVTQHEPSFCGLATLCTVLNALGVDPKRVWKHPWRWFEQDMLDCCRPLESIRKNGITLAEFNCLARCNGLALTSRSPPIGPPEDDPIGYQKGLQEFRSQVATTTALSSAFLVVSFCRASLGQTGTGHFSPVAGYSAEEDRVLVLDVARFKYPSYWVGLEDLYKSMIPVDPASQRPRGYTILSYSPFLEHTNCLTCNPNPPSITRINLNKSSWAILSRELGPIIESMKLEPLPIAIRKIHDYISTGLAKIPNPIEIRSGLESEAQNLFSQISSSIHLLVDDKQTDEIPPILIIFALAVLPSHPHALDLEFLEPELAYLRSQLNALGECCSLENDHEHPQEHRCQCKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN74</accession>
    <name>A0A5B0QN74_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like-conjugating enzyme ATG10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Autophagy-related protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_014244</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN74"/>
    <dbReference type="OrthoDB" id="4089664at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061651">
        <property type="term" value="F:Atg12 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14957">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14957:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1114578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="244" mass="28689" checksum="519BFB77C2692B0E" modified="2019-11-13" version="1">MLTRVEFNQGSQRFINNSTDTPNRCNRGWKWKQYQNRTSRIIIEQDLGCLYHEPVDRRLFINTQDSEKCKEEEEGIVHEQEDEAATIRIPDCRPYVPIKYQASVVFSDTYRVPQLLFQAYKPDGTQLNLDELIHTDIFYGDSLEYPMNLIKSSTSSSKEDKDKEEEEEEEEGKRVQLPILTRTIHPIDNQTYWGLHPCNTQTILKEILEEEHRNHPKKLPPQLVIESFFALVSSKIRPPQHCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNT2</accession>
    <name>A0A5B0QNT2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_023822</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNT2"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023030004" description="DUF7872 domain-containing protein" evidence="3">
        <location>
            <begin position="30"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="4">
        <location>
            <begin position="241"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1114800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="458" mass="49053" checksum="E41E23C93BB5BA94" modified="2019-11-13" version="1">MVRRSPVQSKLTPETWLLLSSIFMTTVNAAGDPTLSRRNLFPSAAKSSPGATTVQPQATGPAASGCVQPPLTNTTWIQLGIDNYLANLPNGTSLNLQHYAASVGAPDFICGIGEKCQAGQLCSPVQAPGWEVLVAAQEWNNYQNQFYEAVGTAVQMVTSISGALVVDLYPPSHTGGIWDIFNSFNLASGITAGVVALLVLLVGAGAVIATPLAILSLSAVAVTFGVTGTISIQEALKHRGTNAFDKFSVYAFYLSEWQKHAQKSIANYTRSIIASGVSSPQGISTVLKNGTFFRERPSLNEAELVSSLRNTTTVRIMTDILRSQGAFVTIASDKCTGKGPGGAWKGTDKLSYCSPNGTMFNIIHSGKKKVLNTWWNADLIAKKYGISVQYLAEQSYFCQLNHGTANFDPYKSSAFPKDLSAECISNLPVCDFRNPDVAEAKKEKNTIHACRNVAKLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPL5</accession>
    <name>A0A5B0QPL5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPL5"/>
    <dbReference type="OrthoDB" id="10576683at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022710537" evidence="2">
        <location>
            <begin position="24"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="846" mass="96918" checksum="28E5DC9AB01CAADB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPU0</accession>
    <name>A0A5B0QPU0_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPU0"/>
    <dbReference type="OrthoDB" id="10309861at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="11528" checksum="F85DA3BC036AA48B" modified="2019-11-13" version="1">MSTLIGAKKQLEKKTSEQQKCWNSIPKSSPHKRRASEISCSRDERATAKLKACDSDDVIMLNDEDHRSSNSNLHQIPSNHTPLINRIFSPKTEASNESRLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQH6</accession>
    <name>A0A5B0QQH6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_032093</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="2507150at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051480">
        <property type="entry name" value="Endocytic_GEF_Adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006:SF6">
        <property type="entry name" value="INTERSECTIN-2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16652">
        <property type="entry name" value="PH_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="640"/>
            <end position="744"/>
        </location>
    </feature>
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        </location>
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        </location>
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            <end position="845"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
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            <begin position="61"/>
            <end position="71"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="72"/>
            <end position="102"/>
        </location>
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        <location>
            <begin position="110"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="255"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="301"/>
            <end position="316"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="379"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="821"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1115133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="845" mass="92780" checksum="003D879DC00BC82F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSZ2</accession>
    <name>A0A5B0QSZ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSZ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706">
        <property type="entry name" value="SLR1338 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706:SF1">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="246" mass="27329" checksum="2BE3CFD1230A8588" modified="2019-11-13" version="1">MHAPPPDYSVVDPQFAERRRYSFHPILQGRYNQQQQQQQRHPLTNPNEPSSFRRTLSSLQSLLIHPNRSPNPRHTISNGPQLSQHQRLGRLVIGSGEALALNSPLNQTPASPLLCTNPSSGEDKLVILARYDTVLLIDDSASMSQQNSWNEAAVAVAALADQLIGRKPSRWAGFKSIQEPSYLGSWLACKPTRRRGGFFCTPLGSVFFFQKTRFQGAWAWACGEGEGVCLSTPSCYLSRLKQLGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTP8</accession>
    <name>A0A5B0QTP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
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    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
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    <sequence length="804" mass="87434" checksum="20BE1FA3068E2AED" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0QUN6_PUCGR</name>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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                <person name="Pretorius Z.A."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0R0E8_PUCGR</name>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Silverstein K.A.T."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Proteomes" id="UP000324748"/>
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    <sequence length="103" mass="11252" checksum="D9227F27697BCE6B" modified="2019-11-13" version="1">MRAVTFAELKVPSRVGDLTRTTNAINTLASGQCQIGKSALVKVDWRLRFDTFMATPQAHSHSTGFGDSSSTGDRLTRANLRSTNQKLDFGLQMPAKFGDCSAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0J7</accession>
    <name>A0A5B0R0J7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Blue light receptor</fullName>
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    </protein>
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        <name evidence="6" type="ORF">PGT21_010524</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="PAS_fold_3"/>
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        <property type="entry name" value="sensory_box"/>
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        <property type="entry name" value="PROTEIN TWIN LOV 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PAS_9"/>
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    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <end position="471"/>
        </location>
    </feature>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1118929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1110" mass="121632" checksum="5F7695A78B2B0ADA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2C4</accession>
    <name>A0A5B0R2C4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001157</name>
        <name evidence="2" type="ORF">PGT21_031557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2C4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="90" mass="9649" checksum="4EA4D996B8E2A25E" modified="2019-11-13" version="1">MGDLNSFGLGVILDSALALLRGSSLPDFSHLLIDLASGRSSTGSIIHPRRKTAAIECASPSSTVDKPKDPLVQLFDLDSWISDDPQPIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4P9</accession>
    <name>A0A5B0R4P9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4P9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="108" mass="11878" checksum="8F813C8A9DA5564B" modified="2019-11-13" version="1">MPSGFVKLTDKRELGVGRPAIDKTSATLTRMALPRAHNPTQKPLYRAIPLALKPNLSPISGPRAQFFSFSFSLGWIPRCGFAVEVCLIDLGIDVCRVGDRDVRVKKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4Z1</accession>
    <name>A0A5B0R4Z1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_037120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4Z1"/>
    <dbReference type="OrthoDB" id="3264316at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="6">
        <location>
            <begin position="104"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1120075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="258" mass="28623" checksum="C0818D1C92B0CC47" modified="2019-11-13" version="1">MSERVKKYLAEAMESSTLVLATMMHPCYRIRLFELAFGADSSEVINCLSLLQLQYQRAKDQAPVALQGTDPDVMVLETTMCFKPDSLMDRLSRAQKPLLDENEIEAYMKADISFKAEDLAHKSTPLKWWKAHHETYPILAILAREYLGAPGSSCAVERLFSAASDVCRTSRGSLLPSTISHSVSSLMWLREEVPLSGEFDEAGRCDSVAAGKNTARKENDKVAHLRYRKSSANDQKSATVTGSAVTQCCFKQCQNPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R8G5</accession>
    <name>A0A5B0R8G5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028703</name>
        <name evidence="2" type="ORF">PGTUg99_032951</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R8G5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1121588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="52" mass="5827" checksum="98BFC9408D726102" modified="2019-11-13" version="1">MSGSTEKSATLRVRETVDVSYNPEGEWYSVAGIQAYRHQIKVSFGMCGKRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RM52</accession>
    <name>A0A5B0RM52_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_034970</name>
        <name evidence="9" type="ORF">PGTUg99_029738</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Cd(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48775"/>
        </cofactor>
        <text evidence="5">Binds 1 divalent cation per subunit. The enzyme is active with manganese, cobalt or cadmium ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-II DAHP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RM52"/>
    <dbReference type="OrthoDB" id="2338at2759"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00084"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002480">
        <property type="entry name" value="DAHP_synth_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21337:SF0">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21337">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01474">
        <property type="entry name" value="DAHP_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0104">Cadmium</keyword>
    <keyword evidence="5" id="KW-0170">Cobalt</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="263"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="316"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="483"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1106441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1126372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="521" mass="57697" checksum="ED6CCFBDE3F1A722" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RYN8</accession>
    <name>A0A5B0RYN8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032436</name>
        <name evidence="2" type="ORF">PGTUg99_008104</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000111">
        <property type="protein sequence ID" value="KAA1130215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RYN8"/>
    <dbReference type="OrthoDB" id="10320986at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1081261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1130215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="245" mass="27757" checksum="FA6451E2A71988C4" modified="2019-11-13" version="1">MMDPDQFFLHTHQPPSKKSLAIPEDTEENFKHILYSVTRNVWVPKSFNPISCSSLPPLDGPVYLNVAIELIGKLEDPQETIIESIIQTILTTLYQFSKGTTVCLTKEPLKVLLAQQNIGRLSSLQLDVLFEAQRLSHPLSFTELMKQLTFNLICDLMNHLATEIPLVFNSKWRKSLPKKLSTIIHMSTYTTSIADSAKDEEARSEVGKIPVKLDMNQSDQQMVINSQSKINSQLVKVFLHHGRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S6E3</accession>
    <name>A0A5B0S6E3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028075</name>
        <name evidence="2" type="ORF">PGTUg99_018689</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1132985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S6E3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1132985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="99" mass="11186" checksum="66C95DEE4E9DB143" modified="2019-11-13" version="1">MVTRISLSMTDFIIQLTIISGSLFPIHRCHASLTTNGFSHEEPDLADHGPFTKRLVNSPIGAHNLSRVNIKRRTKVCKKINLVRQCRSSDSKSRKTRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SA82</accession>
    <name>A0A5B0SA82_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025974</name>
        <name evidence="3" type="ORF">PGTUg99_008144</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000042">
        <property type="protein sequence ID" value="KAA1135016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SA82"/>
    <dbReference type="OrthoDB" id="10538495at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="255" mass="28365" checksum="16162BE182082F0F" modified="2019-11-13" version="1">MAHKATKSVPANPADSSRHEMSNTPNSNSPQAGPSRQNRAPDESTPRPISSESQSLDSWYTESSSSEHNSISSPPNNPSDNQFPKIEPQETALRYHPIFPRGSVINVEVPEVVRSETQRYYFQPSSCQVADPRSRVPIYFRWDGDWSLERWAKFTGEVSSEPGAVPNAIVGPDAMARQIHEERYQEECRQIRPPGLTGTAAWDKQMKITLERFFAPLETEDEDIEAEAEGIAAEPQVEGLEAEAGDIEAEADDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SGB9</accession>
    <name>A0A5B0SGB9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003768</name>
        <name evidence="2" type="ORF">PGTUg99_004174</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000015">
        <property type="protein sequence ID" value="KAA1136917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SGB9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1136917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="50" mass="5601" checksum="035B2F22B8B76492" modified="2019-11-13" version="1">MSAVASDCLRFPNAVKDQLNFTQNTSRYILFLSVNNASSHKAPRPTFIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LIZ0</accession>
    <name>A0A5B0LIZ0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LIZ0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="631" mass="70502" checksum="BFEA9E9114D10752" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKH3</accession>
    <name>A0A5B0LKH3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKH3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="87" mass="10059" checksum="03AC84B7B87BFF28" modified="2019-11-13" version="1">MRRDHYILKRPRDESSSARGQSHLPHPRPRAENDSARDYQQNDLELKTIQLEVCPSESDYRGTDPELSLLQLEVPFLSLTLLRPTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLG2</accession>
    <name>A0A5B0LLG2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLG2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1064753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="79" mass="8559" checksum="FFD01CA421043F2D" modified="2019-11-13" version="1">MGGYPLGYLDIRQDFRGKGASAPAGGYPLALAGIRQQITDIRNGLSATISNDYYTSAHCFCALQLFNSCHPAHLRRALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLW7</accession>
    <name>A0A5B0LLW7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLW7"/>
    <dbReference type="OrthoDB" id="10268239at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022846116" evidence="1">
        <location>
            <begin position="24"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="123" mass="13740" checksum="50C2EBC4AAC0AD98" modified="2019-11-13" version="1">MARFAWVLSLLLTSFLVAPGIQGPPTGPYRYTDRCERCWQAAGSPTDFVNTMYKTVRYSSYPCYAFGHDLTCRHKFAVQGYYCVGCCNLVHVSLGRCPTHGQSTQYIPEPRLTTPQAPRPAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNM2</accession>
    <name>A0A5B0LNM2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016914</name>
        <name evidence="3" type="ORF">PGTUg99_010769</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000108">
        <property type="protein sequence ID" value="KAA1130503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNM2"/>
    <dbReference type="OrthoDB" id="2496952at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="125" mass="13995" checksum="7AF98A4010A3F9C2" modified="2019-11-13" version="1">MEEYRAELAKKKEEKQLEQERARAEKGRKNWRGRGSQTPGGGGSQTSDSSQSQSQLDSNAAFTIEDYELICSYLGVPDNYSKLYGSGDQTDFGPRPLTKTAAYKMFAIYMNSNCNKQYKLTGAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPD9</accession>
    <name>A0A5B0LPD9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_007496</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPD9"/>
    <dbReference type="OrthoDB" id="2108at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="8"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1065680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="323" mass="36238" checksum="5C947BADA9E5740C" modified="2019-11-13" version="1">MLSVFRRLVPIPSALSAEDNYKLHIDKLKENINQMGLSVLFASNPRNPTGQAVEGSELEELVQVSRNGQTVVLDEFYSWYNLDGQLGESLSAAKYVEDVNKDALVIIDGLTKNWRCPGWRVCWVIGPKPLIGALSQSGSFLDGGASHIMQMAAIPLLDFDRVEKDKLALQVHFRMKRDRVLERLEQMGLKVKIPPKWTFYIWLDLSDLPPPLNSGLVFFEELLKQKVIVVPGIFFDINPSHRRNLFCSPCHHFVRLSFGPPLPQLEKGLDGIEQVLKKAYDHLTTDGHLLEMGKNLAPQLNRRLSIAAVHGVADSSKLSSPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQ00</accession>
    <name>A0A5B0LQ00_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQ00"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022756448" evidence="1">
        <location>
            <begin position="21"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8311" checksum="5DA028A38C0DC436" modified="2019-11-13" version="1">MNATIYALLCLAAAAANVATVGEKMKCFTYSAGTKGYSCNDRSDIICTEGCKTFAKMADWHPDIRADADIRLLFPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRN0</accession>
    <name>A0A5B0LRN0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRN0"/>
    <dbReference type="OrthoDB" id="2507923at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022804706" evidence="1">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="11223" checksum="69664C953162A9DD" modified="2019-11-13" version="1">MHVFKVALMMMAISVDRLATAYDCGKDAPQNVCEVSHGDLAIYVRADKIPVRLDKNLSFPAGGKNCVEYGPHTRRCCPLATIPAGSWISSDRYKQAKCHKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LX93</accession>
    <name>A0A5B0LX93_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LX93"/>
    <dbReference type="OrthoDB" id="10370228at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="198" mass="21706" checksum="AE1E073CDC7AFCEA" modified="2019-11-13" version="1">MDKDRLKRILRAFGEGWVELTGPPAPQSGEVGGVLEDEDGCTPQSDNASRVRTARGSGTARGSRTARGSRTAGGFRTAGGFWTARGSRTARGSRTARGSRTTRRAQTDSNRIHMKKAKHSSAQQHYSENRTRRKRLIIDDTDDKDKASPVQTPQQAKKKQRVLCPEDNQEDSSMGGLRHEDLHSDGDNSSNGSQLPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZU9</accession>
    <name>A0A5B0LZU9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024576</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZU9"/>
    <dbReference type="OrthoDB" id="2507165at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="162" mass="18559" checksum="2F8D94C15F7039CB" modified="2019-11-13" version="1">MGLLQDLAKHERGALRNAEFNRHPIHGPPPPLMDLILIVNRAMGGQSQLRLVDQILREYTPSMRIRLAYVRLEVIHHFLHPNRGSNLTQWELINRQLEHVRSQSENYKNAYAQLIVNLDRQLFGDQLFQDIPVALIVLPTEVQVSEQMAIAGSTPRRTGGPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M098</accession>
    <name>A0A5B0M098_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M098"/>
    <dbReference type="OrthoDB" id="2496464at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="417" mass="48529" checksum="844B24533ECFF580" modified="2019-11-13" version="1">MDGTLAPRNGSMNSDGKSEEENIELTLKILSIVRQRISSHSKDDEDDNRANLLYWLELRNTVPYELRQTILPRLHEMIQSATMMLNEFDPTTINPDWWYRRFLIRLMDIAELVEQINSSILSIWTTCGPEQEAPHELPEVEHLTRSRCTRTEFLVQCLIKYDLDDLFGSFEDLFNGLRPTTSSGSLPTASQDSGQIIEGINRRMPPVVDRINSITQWLQEPLVNIAKADWTNMADMFERGLFYSTYRTKFASEASFLQAFTTFLKFARLLVTKLPQSAPNQPLFFFGPLMDIEDVRLKILMYYSDKIGIWMSTMGFKIQNPHQPIDFREVATVLIRVIGGYSRFLIILERYWDSLLESDAPQVDPNLIVNARQWLNSWTPLFTLAARKVMQATGRLHFHQFHFELREAEHDDFLMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0B1</accession>
    <name>A0A5B0M0B1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Phosphoacetylglucosamine mutase</fullName>
            <shortName evidence="12">PAGM</shortName>
            <ecNumber evidence="4 12">5.4.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Acetylglucosamine phosphomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 12">N-acetylglucosamine-phosphate mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PCM1</name>
        <name evidence="20" type="ORF">PGT21_026547</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, which is a biosynthetic precursor of chitin and also supplies the amino sugars for N-linked oligosaccharides of glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23804"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="5.4.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12 15">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.3" evidence="4 12"/>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0B1"/>
    <dbReference type="OrthoDB" id="1928at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004610">
        <property type="term" value="F:phosphoacetylglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03086">
        <property type="entry name" value="PGM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000003">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000023">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000038">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049023">
        <property type="entry name" value="AMG1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049022">
        <property type="entry name" value="AMG1_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016657">
        <property type="entry name" value="PAGM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955:SF1">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21405">
        <property type="entry name" value="AMG1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21404">
        <property type="entry name" value="AMG1_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016408">
        <property type="entry name" value="PAGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="17">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="19">
        <location>
            <begin position="183"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="18">
        <location>
            <begin position="297"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="16">
        <location>
            <begin position="443"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="13">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="15">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="374"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="488"/>
            <end position="492"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR016408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF21404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1069500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="533" mass="57924" checksum="E11C43C5D2D3E050" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M224</accession>
    <name>A0A5B0M224_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_033838</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M224"/>
    <dbReference type="OrthoDB" id="5392716at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="225"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="493"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="501"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1069824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="523" mass="59840" checksum="CB9BB9EB74E7E375" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M4L3</accession>
    <name>A0A5B0M4L3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">U3 snoRNP protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">UTP20_2</name>
        <name evidence="3" type="synonym">UTP20_1</name>
        <name evidence="3" type="ORF">PGT21_004387</name>
        <name evidence="2" type="ORF">PGTUg99_009286</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000482">
        <property type="protein sequence ID" value="KAA1070940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M4L3"/>
    <dbReference type="OrthoDB" id="360653at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052575">
        <property type="entry name" value="SSU_processome_comp_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046523">
        <property type="entry name" value="UTP20_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695:SF11">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20416">
        <property type="entry name" value="UTP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20" evidence="1">
        <location>
            <begin position="5"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="269" mass="29846" checksum="0930E04D77244B67" modified="2019-11-13" version="1">MIQLVLPESASQNAISGIISSLSNILKSTHQETRQSARATVGNIVILLPASFLAQVLKDLKAVLLRGPQLHVLAHTTYSILSRASESSKDFEIDSHASKLLMSIIIDDLFGQPWRDRQSKELKAKKKFNETKTSRSLESLQMIVSKLANSENLTDILVSFQQVLENTGPTKILKQVDECFNRICAGIVANKEKFDAPKVLDLSKNLISENAEYLKNKSQKKKSYVINPTDHRVVLSTKSKGFYTTSSEGTPSDKLVHNPARREGFLPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5C5</accession>
    <name>A0A5B0M5C5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cms1 ribosomal small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026159</name>
        <name evidence="4" type="ORF">PGTUg99_014755</name>
        <name evidence="2" type="ORF">PGTUg99_028053</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000416">
        <property type="protein sequence ID" value="KAA1085186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5C5"/>
    <dbReference type="OrthoDB" id="1929311at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032704">
        <property type="entry name" value="Cms1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030:SF0">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14617">
        <property type="entry name" value="CMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1085186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="40528" checksum="10ACF044BA78FD9B" modified="2019-11-13" version="1">MPPTKSTKATVSADALEDTQFSINGTAPQDDGHSENEPPKKKRKKANQKAKKTKKATDPPSTQNDAEAAQDEDSAAQANDDDTTKEDLKSPVKPGVTPSSSSKLTPAAIAELKRRKKEKAKTKQKLRKEKAIQLKGVKEEQCLHIHPACESTNESSSIGLQPPDLQINYLKDRQKRALPLLSDLEFESLPLEKSWLVDVSDKPLRGHLGAWLETGAVPGLLEAVKETPDKVGAPVALVISSSALRAVDLCREVKRLIANPKESGEITKLFAKHFKLPEHVNHLKETKVSIGVGTPDRISKLLTYKDDEGLKLDRLKFLILDLTWLDAKGFNLTELPDSTHKAALWKSLLGLPKTLERFQSGKTKIVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9Q8</accession>
    <name>A0A5B0M9Q8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014826</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9Q8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="402" mass="46841" checksum="12FA67B5CAF08DC0" modified="2019-11-13" version="1">MLDSNSEMSKPDRPEVPEELQRKADLIIRDFRRLAEQYIHPWPINIAKKDISIEHLRSKKDLLTGLDSRLLPRLQKQCVSLASLLHDPSHLREDPTLTLNLISETQTNLHLILCLLHSNLEEIFPENPPSPCQKNDQHFREFKAYRLYQFDRSIRKGLKDGLQKLYQESIQIIEEFKLSTKNHTNFVQEASYSVNESIDIAIGWSKGPDLNFVWDIWQDTIVEFDSDLQELMIQMSLEKISDGNPEDPLSQPTIEFARSLIPIIKLTRVFFTKLFREESRKEKAEFFTTLCSDQLNSLNTSIDVISDYIFALPIYIKNAHKFPRIVTTSGIIEGLNKAVSHFLSYFSIVKIDILPNLFPKPEDLLPQIQLQQWFDTWYELFHDAFHIVHHAADSFDQISSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9T8</accession>
    <name>A0A5B0M9T8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000166">
        <property type="protein sequence ID" value="KAA1072614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9T8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="252" mass="27020" checksum="4F3F2BA189070CF3" modified="2019-11-13" version="1">MPLSLDLLPASQFKNSHDLRVHQEVDSCCGDQFLIGGKPSVNSCLLPFNCAGECQLVICPRNGQAPLLLVAASTLVQLSVLLRSSRLSTSNIHVSAPSGPQTSKKSSHPSAASKSKEVRSSQRVTRGSSGTSKPTSASKSKDVISNTPVPPATKDVAPASTDVPPATDDVPPATKDLPANSTLNISDQRFSGRSINQCAFCLASIWEGVAEFDLARKNQRMLKNKDKDVVDEGVDELEELSDKEEAPKKRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MA99</accession>
    <name>A0A5B0MA99_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_010486</name>
        <name evidence="10" type="ORF">PGTUg99_017911</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MA99"/>
    <dbReference type="OrthoDB" id="442352at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302:SF5">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="555"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="604"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="651"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="9">
        <location>
            <begin position="98"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="399"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1074107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1090677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="673" mass="73428" checksum="D171FA2A49A103D4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MD92</accession>
    <name>A0A5B0MD92_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MD92"/>
    <dbReference type="OrthoDB" id="2509314at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="428" mass="48241" checksum="D0CA2D9ADCAEF366" modified="2019-11-13" version="1">MTPALSLDETQSEPVIGPSTIPNQEPLPDEPGQQDGQEGMQIDGPPESLNPQSEPCSAPLNPPPNPTRVVHLAYKISTSQSDAAHEPTIPVPPIDIGFENLSFKSFKERVFDQFRSHQPISHLFQVLVKADASEEIDWNYSISSPLDASRTWSLTTRFNWAFSRFLVAAKCFPSDANISVELRIVLPKSDDEPAKLGQHAIPPETSTPDVQPSRTKPASPARTNPAENQPTPPTPAPRAIPAEEIPPQELPQEERPPQDLPQEERPPRDMSILDFMEFCHIPRDDTYVLALFTLHQIHHWSVFERVSEERLLALGFPVGPARHLTMGVAEHLTTRTHIPTHAPDLPRENMTMAEFLRFCHIPPDDKHTQAQITFHEICHWTVFQYMSEDELLRLGFAFGPSRLITRGVAEARRKLRRNRSPSFDHFMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDM6</accession>
    <name>A0A5B0MDM6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Survival protein SurE-like phosphatase/nucleotidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_004833</name>
        <name evidence="3" type="ORF">PGTUg99_031439</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDM6"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027746">
        <property type="entry name" value="TTL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47551">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE PBY1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47551:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE PBY1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="356" mass="38211" checksum="651A09CCC6147FCD" modified="2019-11-13" version="1">MSTTTTRGCPEVPTVLLTNDDGPCSEEESPFIYSFSELLIAEFLGSERSKLKVVIPDSQASWIGKSYLIKNKLTVVDYDPSTRTKSTDPSSTGPTALPDNPWKLVSGTPASCSNLGLFNLFPSQIDLVISGPNYGRNTSSAFSLSSGTVGAAMDAALSNHRAIALSYGIFERPISDGLLKAANQLAVKIIRELWKTGFGQPGDPNYPDLYSVNIPLVPAILNEPKVMWTTESRTRYARLFLPATDGSKSEPTQPAEIDEAASHLQDLHSNDPCGSNPKISKPLHGPLEFVFKPDISALIDPNAPGLLEGSDAWALNRGFATVTPLCAAFKPAPRPPIPDPTQSASTTDHGDSVWKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDU0</accession>
    <name>A0A5B0MDU0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033581</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDU0"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038921">
        <property type="entry name" value="YOR389W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204:SF1">
        <property type="entry name" value="ENTEROTOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204">
        <property type="entry name" value="YALI0A21131P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="548" mass="61984" checksum="9A1EEDDBF3E57C26" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDW6</accession>
    <name>A0A5B0MDW6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDW6"/>
    <dbReference type="OrthoDB" id="446723at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052651">
        <property type="term" value="P:monoacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF194">
        <property type="entry name" value="FI04476P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="144"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="417" mass="46331" checksum="D25E8001BFB2E408" modified="2019-11-13" version="1">MGFLYNCHRWLLCLPVAYVVFILCLGIPYIQRGAIYLHWVRIPVQANFDAPEFYGIPPGQVRNFQLTTPDGAKIGVWHALPEAVYVRHFQQSENYSGSLLQSDTEAIPSSVFENALRTHKSVLYSHGNAASRAQSSRASLLRAFSATTYAACDEGLNFVIYDYRGFADSSPSSLFPPSEEGLIKDARTVWDYMVGSGANPRNISIIGQSLGTGVSAALAHRLITEENVTPNSLCLIAPFTSIPELLKTYSLFKFIPILQPLSVFPKAQSWLLDTFLTTHFNTKAIIPSITTNIMLIHAQDDIDVHPSHSKSLFEYIHEHHSTNVTIENRADLPILLPSPHKQSHLSPTPIPPSSDDRFKPVVTTHKVSSFGKLHQFNRIRSGSQVIFLDAQRGGHNWVGYSGTSLRAMTEMICKPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFF9</accession>
    <name>A0A5B0MFF9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFF9"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="41"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="537"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="623" mass="66905" checksum="80FE9CB5CEFF6E9D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHR9</accession>
    <name>A0A5B0MHR9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquitin fusion degradation protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">UFD4_1</name>
        <name evidence="2" type="ORF">PGT21_000414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000147">
        <property type="protein sequence ID" value="KAA1075758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHR9"/>
    <dbReference type="OrthoDB" id="271273at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="231" mass="25166" checksum="948AEF5E7088DBE2" modified="2019-11-13" version="1">MRCTPNNEKYSFHETGPAMSIYTYLVGPQHEYFSHQSAHLDQTRQSSAALIWSNRTSKIAKAAIYATRKHSTTSETVPSPPPATVSPSQSPPQPLPIAAVPSPATVSKWSEMYSQSRATFFLTRIKECPNKPFLPSPDDNTFSAILKSFTNIGRSSPEVTTNLLDAGLGDTVYGLLVGQTPPEIPDEHDLALELTSNSQIQQAIGLVVEVLPPLPKSGIFDPRLAKKPINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIT0</accession>
    <name>A0A5B0MIT0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIT0"/>
    <dbReference type="OrthoDB" id="2509616at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="405" mass="45402" checksum="6A02B0D117883522" modified="2019-11-13" version="1">MRGGDGDSQGSAEDEKSEPEDPEDILKYNLKAQREKRAHEKSQVNEQRVQRVVVGKGLPNQSTALCRSIRNFTIFIMGMPESISAYPAPPDQEEWEVWNNWTENRYKKLTKHLEEFSEKNKNLAKSVFQKKACEQDLQIAGFGHLTFEWQASSFNSSLWSASLATIVIKHYFNLVKSKPGGMWGEVGCMEQILDRWVCGQGKEIRRAHQTNGQSLEDLKAEKSRKAAISRMKAKIANKRQGLAITKMKAEPNLAVLFSKEAVSDWEDQPVPGKPKRIQLAWRSEAFSLCSHKLDEIAITVAKTTTEMKTIHKLVDRSPELITHSTTPLAVIPRRLPIDAYDAIFLQSLSQVKKDHLDPQKEIGLEAKAWMLNQMTMRIGPGASQIPRGSIGDLGIGNLPGPSTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJS0</accession>
    <name>A0A5B0MJS0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036227</name>
        <name evidence="2" type="ORF">PGTUg99_037434</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJS0"/>
    <dbReference type="OrthoDB" id="2502440at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="209" mass="22025" checksum="8C7EB1B9E8571B40" modified="2019-11-13" version="1">MLIINCYTLSFIGFYNLINYAFTTSYPLEIAPAPDGRLLLTGATKFSQDLTKRQNRGTGTTNTRPRPAVTTAATQKAATTGGSNKSLKLREAARRLNTEANQVTKGLQDLKKLNPQSVSAAVDRIMKIVAAEAGPRDEIGKALPNNSAVQSNVAAIKKAGPSLGAAIRALKTGGTGANFSKQVDKVLEIRKPTLEAVKSIIGAADAASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKP4</accession>
    <name>A0A5B0MKP4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002399</name>
        <name evidence="2" type="ORF">PGTUg99_004219</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000452">
        <property type="protein sequence ID" value="KAA1077192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKP4"/>
    <dbReference type="OrthoDB" id="2506545at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="706" mass="78151" checksum="660AC64E00A0EAE7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP79</accession>
    <name>A0A5B0MP79_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MP79"/>
    <dbReference type="OrthoDB" id="2513296at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF22">
        <property type="entry name" value="TRANSPOSON TY3-I GAG POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="658" mass="72085" checksum="AD4FD2ABF2504BE1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQN4</accession>
    <name>A0A5B0MQN4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1764-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004677</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQN4"/>
    <dbReference type="OMA" id="NTDDCPF"/>
    <dbReference type="OrthoDB" id="2499051at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013885">
        <property type="entry name" value="DUF1764_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34066:SF1">
        <property type="entry name" value="DUF1764 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34066">
        <property type="entry name" value="GROWTH FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08576">
        <property type="entry name" value="DUF1764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="19411" checksum="503E51C0AD0C6EE6" modified="2019-11-13" version="1">MVSKNLSEIDAIFSSKGTNNSTTGKKAKARPPDHPGPSDGEGSKTGRSRASGASDGKALASGEERRLNNKKKIPSLHDRPALKRRAEEEPSVEVLLDPSQALLHPPPALDPNCLKKKKKKGNGLPSTLDLDDEQERAFRDSRGTRAKTEDGLMIYSVEELKIGLGQDTPECPFDCQCCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRZ2</accession>
    <name>A0A5B0MRZ2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_022766</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRZ2"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF12">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="816"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="881"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1009"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="819"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="883"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="893"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1019"/>
            <end position="1033"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1079737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1141" mass="127859" checksum="0E5ECB7042B06E4C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSM0</accession>
    <name>A0A5B0MSM0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002737</name>
        <name evidence="2" type="ORF">PGTUg99_014679</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1079965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSM0"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="153" mass="16640" checksum="3B96F2D4B9EEFC54" modified="2019-11-13" version="1">MQNEPDPNYIDPLIALAPTPTNLSRLAATATTTDKLIQIKQVFTNAYLRAASTGDSDLLEWLLNKTTTPNEHPLSPSPSSSHHITLLKPGQARQWIDLEARDEDGSPAIILAAAFGHAEAVRAIVDGLGDQVVDRRDAGQYFLHTPNNKPIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTS9</accession>
    <name>A0A5B0MTS9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTS9"/>
    <dbReference type="OrthoDB" id="2506580at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="88" mass="9472" checksum="4C3D44E9CB3D3A67" modified="2019-11-13" version="1">MSQSNAFKSPASSSSSSSSSSSSSTNSAERMNEDSSLDQHRRTRSQAHFFYTREALLQLRPAASTDGQHPFGGNNHSSIPAGLFVRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUH7</accession>
    <name>A0A5B0MUH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025134</name>
        <name evidence="3" type="ORF">PGTUg99_020519</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000339">
        <property type="protein sequence ID" value="KAA1100728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUH7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="78" mass="8662" checksum="AA96361A66C001E1" modified="2019-11-13" version="1">MRQGRGHSHANAAHSREWGLVLRSAHLAVLTVPPHECLQNSIDMKKGPLTSLPRVARPRDFTQRTFQGHAEPSGTTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVV8</accession>
    <name>A0A5B0MVV8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad60/SUMO-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008329</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVV8"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01763">
        <property type="entry name" value="Ubl_SUMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad60/SUMO-like" evidence="2">
        <location>
            <begin position="167"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="234" mass="25857" checksum="083B4AF9EE4DB89C" modified="2019-11-13" version="1">MTCSARKTYCASLCGGDAICAKRPNGLKNQICTRRNLGSFLGVDCARCSGSTRADVLGFGGTCWDIPRDQLILQPLVVSSSPTPPRQTPENQHQKQDERPGKTKKPTWKKKRLSTYSRDVRFEIVKRLCLASYSSKRPCPTRPPSPDQTSSPKSPQKSPSRFSPDGDEPLILRVKTTTAFQKIFNAVATQKNAALGSFRLQYDGQNLLPNETTPADLNFDDEEVLDYFVQAIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWP4</accession>
    <name>A0A5B0MWP4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWP4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="52" mass="5939" checksum="3BDB04F5182CEC47" modified="2019-11-13" version="1">MCSADTLQKCTRINQIEGDIIPPDNNRPINPRSIDSRGSLAQSERREERSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXK0</accession>
    <name>A0A5B0MXK0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXK0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="83" mass="9626" checksum="8CED5326DEEFAFDE" modified="2019-11-13" version="1">MHTGGSINGETDPIRYGIGRFIGSDQFFDTRYRKIRKRIAKGDPIPWYRIEMVSDRDRDRIAIPIRHPNAAGCMPYTWVSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N018</accession>
    <name>A0A5B0N018_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_035055</name>
        <name evidence="16" type="ORF">PGT21_035872</name>
        <name evidence="17" type="ORF">PGTUg99_003762</name>
        <name evidence="18" type="ORF">PGTUg99_016001</name>
    </gene>
    <organism evidence="16 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="17 20">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Microsome membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000179">
        <property type="protein sequence ID" value="KAA1125323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000112">
        <property type="protein sequence ID" value="KAA1130185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N018"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF150">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="14">
        <location>
            <begin position="4"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1075427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1081460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1125323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1130185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="130" mass="14167" checksum="C439CF04BC708C82" modified="2019-11-13" version="1">MSEDKDFTFEELSKLSTPSNLHLVISGKVYAVAKFLEEHPGGDEVLLTEAGKDATESFEDVGHSEEARTLMEQFYVGTCKQGAPKGPPIIKKAAQQANPSNSDQQSGGWGFMVPLAILAAYLSYRFYFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2S6</accession>
    <name>A0A5B0N2S6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP synthase subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ATP4_2</name>
        <name evidence="10" type="synonym">ATP4_1</name>
        <name evidence="10" type="ORF">PGT21_004914</name>
        <name evidence="9" type="ORF">PGTUg99_035923</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eukaryotic ATPase B chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2S6"/>
    <dbReference type="OrthoDB" id="67388at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2210:FF:000002">
        <property type="entry name" value="ATP synthase subunit 4 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008688">
        <property type="entry name" value="ATP_synth_Bsub_B/MI25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013837">
        <property type="entry name" value="ATP_synth_F0_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733:SF3">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT B1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05405">
        <property type="entry name" value="Mt_ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161060">
        <property type="entry name" value="ATP synthase B chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0138">CF(0)</keyword>
    <keyword evidence="3 8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1083547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1093967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="241" mass="26677" checksum="909EC6C560892665" modified="2019-11-13" version="1">MNQLRPLARRLALNNTQALRPLVLPAATSIRFNSTTTAPTDPKAKANAFLDALPGNSAISKTGWVTLAASLGGYAVSNELYVVNEESVILAGFVIMVGYLSTVIKEPYKQWADSVIEKQRSVLNASRTEHTNAVKERIASVSEMKDVVSVTKTLFEMSRDTAVTEHELHKMKQKMAVKDEIKSILDGWVRVETQAREAEQAELVKQVVSSVQKQLEDPKLQKEIMSNSIAEIEALIKDKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N300</accession>
    <name>A0A5B0N300_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N300"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="11188" checksum="C5F6CC8B47887861" modified="2019-11-13" version="1">MDMSKSQLPFTLSVTPESRPIGPPVGGGIRSVPTSFEHLANFHVSPYFQNVNQHRKKQPAMEISWEANLARPPQACMKIYLPMLGVMGHGVARPVIVTSQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4C4</accession>
    <name>A0A5B0N4C4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017968</name>
        <name evidence="3" type="ORF">PGTUg99_024336</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4C4"/>
    <dbReference type="OrthoDB" id="9994419at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="930" mass="103549" checksum="6F45C57B15F6FCAD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7R7</accession>
    <name>A0A5B0N7R7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003127</name>
        <name evidence="3" type="ORF">PGTUg99_018129</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000145">
        <property type="protein sequence ID" value="KAA1128066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7R7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137402" description="Secreted protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="18260" checksum="5E0DED3249A483D3" modified="2019-11-13" version="1">MIAFPRLQNILVVIALVITVHPGSARDVSRSESVTCDIFFYPIGIQDKYMCRTTKRDRSGKHQQFKCASSSCKIRTVDWSKYTFQKCSYYIGGDRAKGIDSIKEPNPSITPIQYHANIEKSYVDVQNQADRWWYQCIYSTLVNDDINAARAVCTACDLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBN4</accession>
    <name>A0A5B0NBN4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006503</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBN4"/>
    <dbReference type="OrthoDB" id="2506489at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="140"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="378" mass="42826" checksum="F53DB352E7760355" modified="2019-11-13" version="1">MAPKKNLYKFHYGSQLPVTETRSQKTARLKFAKRTAAEVGRHLQEKNHRERGSDEHRDPEDSQAAPFETAPEHEFNQLGLNDSSPWEDTEEIMDDEDAATLARIRSLHQQLIQQQQNRNWKDLTGVLFPVYLHLKKITGNWTLPCHFQNFSSLVCSCTAEMHTSREMDLVDLMGQMRVKFTFCRCTPDPVHLLANGYLASTPIFPQTAFSLRLLNFYDLLWNICNSHVTPFTKVLQRWNESISGRLCAKKTSKPRQLRRNLSASVDIYRTLKSMERNFFQTITSKNKQDILAQRSCPACFGVSHIVPNQPPSADTNNQTNTTNSTSNNTSPAQPLNSSNTTRTRSPDNTHTSDQGVDKRTPPPSPHAHAPAPPKGACN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NCF5</accession>
    <name>A0A5B0NCF5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent DNA helicase sgs1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SGS1_60</name>
        <name evidence="2" type="ORF">PGT21_020095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NCF5"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="155" mass="16587" checksum="E750E332D7385587" modified="2019-11-13" version="1">MVGVAVPQALEMASDTELKKTISQSGLTKYGQECKKLQVESVLSLLRGKNTFILAATGFGKSRIPELYLNLLTKDRLGNITGDRQKSDIIPKPLPAVGASFARQRATLRLPAKTQARGLDKLDIARPCKGSEGPSPAGPSISHNPTRVASNSYVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NCT5</accession>
    <name>A0A5B0NCT5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NCT5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="206" mass="24215" checksum="65F2672D779E19BD" modified="2019-11-13" version="1">MEQILLLDLDQLQKSFTYFGLLVGTRYSSLKMINLEITYLGRGQQPTQSQPLEQRPATAQNQQITSHPLPAQGSHLPPPPPPSTQPPQASNYHSQHRQVQHHPYYRQNNSQHHQQSNRFVPNYEPHYQAQVTQTHEPHQQQFRPAQQGNWRGYWRNHRDPTTRLLQVGDYLMRAERVAGRVFRYRGRGRGRNQVQRRGNPQEEPHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND22</accession>
    <name>A0A5B0ND22_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND22"/>
    <dbReference type="OrthoDB" id="2499438at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="100"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="474"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="713" mass="78554" checksum="C4618EF1752F6B5B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGP5</accession>
    <name>A0A5B0NGP5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Wbp11/ELF5/Saf1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_036440</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGP5"/>
    <dbReference type="OrthoDB" id="205569at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019007">
        <property type="entry name" value="Wbp11/ELF5/Saf1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361:SF1">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09429">
        <property type="entry name" value="Wbp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Wbp11/ELF5/Saf1 N-terminal" evidence="4">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="80"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="428"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1087766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="507" mass="54130" checksum="D40B6C4A0EA452BD" modified="2019-11-13" version="1">MAKKAKSLNPADAFRKAQRQKEIKRNKLDRKKAREDAHSKKDSSAIQAEISQLERLSKPTLSEQNRLKEARNELSLILKAKQQKGITTSTDATPSTSKVIIDPATGLPAVTGPDNRNDADKSGHTSSNTTKSGSNSRTMNWFGPDGKLLEPEKSFYYHPVFNPFGVPPPGMPMRMKDSLDVPSGTSSSGPVSSDLGPNDLSQTQPSTSRIIETKGVSEKEGDSENESEEDESDEDTENDEDDIPLPEGSPPVSESEDDDIPLPDGPPPPKPLHKPRSDQLLSRPTAFSENIRIQGHHPHSGLVQPAPYFPPPISLVPGLPPPPMIAGMSMRSFPLGPNGLPLPPPPINHFHSLPPPPTSANLPAKPELAAGPLNGVPTPGAATPAPKPTIQASATVTALPQLRDLKREATSFVPAAIRKKRKELDRRAGLAGLGDGNRIQAAPSSLDPAGEDPSESKEPKQVTTTLLAALKRDGVVSDTPVLKGPVGTVGAEKDDYEKFVESLGDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHH3</accession>
    <name>A0A5B0NHH3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_012664</name>
        <name evidence="12" type="ORF">PGTUg99_024619</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000408">
        <property type="protein sequence ID" value="KAA1088054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHH3"/>
    <dbReference type="OrthoDB" id="17644at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="600"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="727"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="728"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1082739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1088054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="802" mass="90556" checksum="7F3CEAFB754F68E0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHY5</accession>
    <name>A0A5B0NHY5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000851</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHY5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1088935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="15682" checksum="CD3BD35C9DE0A42E" modified="2019-11-13" version="1">MGLINLTTSSPNLFLKRIPSHSKVTDQTPIFIDSSPAISPLAEEMEISILLTPPGYSIYDGDCFTQPEFDTLFAFVMNQITIYCRDNYPKNFLEHEARVKLYMDISRGFKFANVHNLIPKYEDGMPHGSSSGGNHTLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKE4</accession>
    <name>A0A5B0NKE4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKE4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="104" mass="12295" checksum="FD81C0D37196F47F" modified="2019-11-13" version="1">MGWQQEPTVEVLKQARYVNDQDVPGQGGEWTVFDFMFPRCLVDRKDLRRDPGVRHAAGPPPPKLRPPSKSRRPTRPSSTYIVQHTERLINQTRYRQNRRIRPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKH9</accession>
    <name>A0A5B0NKH9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-terminal acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NAT3_2</name>
        <name evidence="4" type="ORF">PGT21_007522</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKH9"/>
    <dbReference type="OrthoDB" id="10264728at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051646">
        <property type="entry name" value="NatB_acetyltransferase_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1089116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="138" mass="15696" checksum="10589517029AC95D" modified="2019-11-13" version="1">MNIAESPNHQIMGYVMGKMEGENEDFHGHVSAITVAPSYRRLSLARELMKLIESASEQQSCYFVDLFVRVSNSLAISMYEHFGYSVYQRVLKYYSGGGVGDHLDEEDAFDMRKALSLDKDRKSVRENGREVLVDTPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NN57</accession>
    <name>A0A5B0NN57_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020534</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NN57"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="54" mass="5673" checksum="597F3735E7BFFC24" modified="2019-11-13" version="1">MPSVDGCNLKTLTIATIGLPSKTSNLRLSPSIYGSAHLGKILATPTITISELLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNH2</accession>
    <name>A0A5B0NNH2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NPY1_1</name>
        <name evidence="4" type="ORF">PGT21_015175</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNH2"/>
    <dbReference type="OrthoDB" id="10249612at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015375">
        <property type="entry name" value="NADH_PPase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF6">
        <property type="entry name" value="NAD-CAPPED RNA HYDROLASE NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09296">
        <property type="entry name" value="NUDIX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH pyrophosphatase-like N-terminal" evidence="3">
        <location>
            <begin position="35"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="253" mass="27651" checksum="D23589992233F0D0" modified="2019-11-13" version="1">MSEGRNYYSGGSTNRLSWMRQSGTFLGSALSSPQARYILFHNLKPLVIDQPSETIDGFPVSNKLSSLPWQEVALMLSSTGSAVVDPGEVVDPTGTMWPKQLASHSANARKPVLDQCPIIIFLGTDNSTEHPEVDKSLQPGHGSPVWAIDVTPIYERTSRTLLQHGRFLDMKKATLALKEANLAAQARALIDWNLRNKFCAACGRPTYSAWAGWKLACTSTLQVHGGTDDGDLATTSPGCVTNSFSIQNFWYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNP0</accession>
    <name>A0A5B0NNP0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNP0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023018721" evidence="2">
        <location>
            <begin position="21"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="144" mass="16119" checksum="A29BC43EFE4EB89E" modified="2019-11-13" version="1">MLPKNILFVLFISFIPGAYSANGTYVQCAVDSCRQRAMELLQHPYPPIERCNEPLGNEDCKNYRQKNYYECDVCKAITIKNTVVHRDDPEPPCTHKGKMLFLGPRVPPPSQAPSQPSSSAGVTPSRTIGKTTYFMFLPEEPPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NRN4</accession>
    <name>A0A5B0NRN4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-type lectin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_013346</name>
        <name evidence="10" type="ORF">PGTUg99_014826</name>
        <name evidence="9" type="ORF">PGTUg99_015911</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000515">
        <property type="protein sequence ID" value="KAA1064171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000381">
        <property type="protein sequence ID" value="KAA1091881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NRN4"/>
    <dbReference type="OrthoDB" id="270293at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07308">
        <property type="entry name" value="lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF45">
        <property type="entry name" value="RE50040P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033473891" description="L-type lectin-like domain-containing protein" evidence="7">
        <location>
            <begin position="30"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="8">
        <location>
            <begin position="55"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1064171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1091881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1092761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="354" mass="39392" checksum="BBD87A7DAE253F90" modified="2019-11-13" version="1">MMPGPSTRIYLGCLKKLSITLLSLQISLAVGFEDHPPPSNQVIHNRQIKGTTEVTIPIKGHTIYPPFVDTDLQNRWFDFGGSAIIDTNRHVRLTQNRASEAGHLWSRYPLTQPSFQVEVEFKIDGDSSSLYGDGMAVWLSKPAQQIGPVFGSADHWDGFGIFIDTFPNARHAYAFPRILGMINHGYTSFDVGQDGDGQEAGACSYLVRRSDVATKLQINYVRGRFLELLVQHDKWDEWDHCFTVSNYTLPENIYLGFSAHTGEVSDAHDILSVTTNGLVYHPPHTPKKGSKSYPEWNGNTAGGIGSGALGFWGSLGAYLYLALKWSFILACVGLALFGGKKYFSKYQQEVMKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NRW7</accession>
    <name>A0A5B0NRW7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031995</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NRW7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="13249" checksum="70A4B5B6C34CFE17" modified="2019-11-13" version="1">MKRNRLLAPARLETLERRTDEAMSIVASRRIDGTYGLCPAKTEAIGFDLIGSKATIHIADRYLGRKSQQELRQKDHPDLRPIICAWLAERTTKYAQNLSPIFSGFFWLVSCIDPNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVN1</accession>
    <name>A0A5B0NVN1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007617</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVN1"/>
    <dbReference type="OrthoDB" id="37537at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19077">
        <property type="entry name" value="AKR_AKR8A1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF78">
        <property type="entry name" value="PYRIDOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="366" mass="41239" checksum="96A12678BE39A1EC" modified="2019-11-13" version="1">MAIDITSHRRVGHETGELVSVCPIGYGLMRLTWAPTETPDEKAFKTILAFLTAGGKFLDSGEFYGNPPDRFNSNLELLARFFDAYPEWAEEGRCFLSVKGGFNLEGGKMNHPDGSIDGLRKSVDNINQKLGGKKKMDLFQMARVDQKVPIEDVMKSYKTLINEGKFKHVGLSEVSAETIRRAHAIYPVSAVEVEYSPWLLDIEHNGILSTCEELRIPIIAYSPLGLGMLTGKIKSLDDLDPNDARRHFDRFQPENFHHNIKLTDKFISLAQKKKCTPVQLGLAWILMQSELLIPIPGSTRAEGVEESLASLKVKLSDEEVKEIRDFVDKADFKGVRYSGQYPFSSTVIYFMYIHPTQNHRTRLPGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZ29</accession>
    <name>A0A5B0NZ29_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022197</name>
        <name evidence="3" type="ORF">PGTUg99_030708</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZ29"/>
    <dbReference type="OrthoDB" id="2496344at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="435" mass="48030" checksum="A71BAE7088CABB65" modified="2019-11-13" version="1">MFGEQATQWFPHGIPESNFGLAPSQGQSDNKILHDRYPYQSEPNPSSYPPDIIAPGLGSFGMFQPSAYPQQQLQNFSSDKQTPEQSIEPLSVSPKSLSFYGPQMTGPLTPTVCRAPHFSQLIDSEIPKNSFGLMGYCSPNPTTKCEQIESANLEYQNAIGYQINNENVGHRKEQINYVFSQNFQSDFGSNMTAVNPPATVGSSQLDQLGLLGPDINNFLDSFFKDPGSMPIDPTRETSVPGLKLPNVNSADEVGLNSPKRNHIGVNTYQRSDKDYQTGVLKPLQNITQQASDKVLVPLQNVAYEKEAKPVYKEHSTYLHSGTPEILELPRIARNPPKHFSGGSGTGQVQKRKLSVEEADQRFISCRTIKTTRRVVTGGKNSRGTSTYCPLITVSIPSVPWIPEVNSGPKTNSKNSSFRRSKLGQALRKKAKIEKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0Y1</accession>
    <name>A0A5B0P0Y1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036716</name>
        <name evidence="3" type="ORF">PGTUg99_029509</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0Y1"/>
    <dbReference type="OrthoDB" id="2507774at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137631" evidence="1">
        <location>
            <begin position="24"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="183" mass="21068" checksum="15E02D048A54B5F8" modified="2019-11-13" version="1">MDPPYGAYNRIWLFLADLNLVLGCLPAPTPYRPVARNSFVSPQNLHWSSLDFCSQLSRKHPTQRSQAWKKAHSTFGREQLGKAGTTSNSCPVHFLTHWSAHVYTCHKHRRPHRSSQKTGQSWSTNQHQSLTPCEQAVKYVVYPYKQDQRLLEDSQGERYTAEHLDHEELSALEILQSIQKTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1Y5</accession>
    <name>A0A5B0P1Y5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1Y5"/>
    <dbReference type="OrthoDB" id="10296859at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022954473" evidence="2">
        <location>
            <begin position="30"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="392" mass="44041" checksum="EC33FC7D24A77691" modified="2019-11-13" version="1">MPWNIAHSASFFNRSVACIFALGNTLAWAKPAFEGLEESYLFGSDLHLSQGDLYDVVYRKRPSANHQTPHQPDFSGGGAHWIDLGAHNNYQVNPEDNVISIVAKRPKTVQLGHGGFHPQSNLGLNALYPGVNQDRMFPEMVSKGAQGLHRSSQDFYQLASFPTDPTSLTSFFPSNDLPSHSFLRKEMFFTPEKEHSNEPTPESMFESMPQREMGPEDDDLKISRLFKQLQSPEFASFAAQKSSSLSDHVQVLSQFPQPKSDVGKYQTHFQDHSPIQYQPHAGKGHQVHNSEDQVKEHPGNQGPLALSSSHAHSRSESSQSNENHRFSSKDSLEEDQAEDPNESLQAHDTDQSENVDITTRSIIPKKPAFLSKMLMLFMVLDEETNSDRYRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5D3</accession>
    <name>A0A5B0P5D3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026445</name>
        <name evidence="4" type="ORF">PGTUg99_009235</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5D3"/>
    <dbReference type="OrthoDB" id="10524006at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="630" mass="72992" checksum="EA3914DEC7729F01" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7M1</accession>
    <name>A0A5B0P7M1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7M1"/>
    <dbReference type="OrthoDB" id="10413477at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="524" mass="56644" checksum="DBCB2AB7F9861C44" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8L1</accession>
    <name>A0A5B0P8L1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003208</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8L1"/>
    <dbReference type="OrthoDB" id="10502636at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023057665" evidence="2">
        <location>
            <begin position="19"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="625" mass="73769" checksum="5F869EA07F8B1E46" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9K3</accession>
    <name>A0A5B0P9K3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031797</name>
        <name evidence="2" type="ORF">PGTUg99_017236</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9K3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="78" mass="8646" checksum="BA6D23330496AD0B" modified="2019-11-13" version="1">MSEDGALTTDAYEPDDFIAARGLRISEVPNLHDLLVESPASDTLDRTQEVHILLEILTAIQLQERVIKDSPSEKGDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA13</accession>
    <name>A0A5B0PA13_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">UMP pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_022396</name>
        <name evidence="13" type="ORF">PGTUg99_036683</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA13"/>
    <dbReference type="OrthoDB" id="10257085at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000003">
        <property type="entry name" value="Uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF4">
        <property type="entry name" value="URACIL PHOSPHORIBOSYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="11">
        <location>
            <begin position="17"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1097866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1117174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="221" mass="24066" checksum="830A5D9F0E2B5096" modified="2019-11-13" version="1">MDSANVPNDDFEDLHISSHPVLKHKMSKIRDRETLPAEFRKLLKDISFILAVEASRDLETESVQGLQSPIAPYTGVALEARIGISPILRAGIGMTDAFLDIFPEASVFYLGLFRDKTLLQPVEYYQKLPAVPQVDTVFLLDPILATGGTAIAACNMLVNAGIPMERIKLITILASMPGLQAVIKDCQGLQIWCGSVDQELKNGMIVPGLGDSGDRLFNTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBA6</accession>
    <name>A0A5B0PBA6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBA6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="273" mass="30438" checksum="E828C22A7EE2B14C" modified="2019-11-13" version="1">MNPSPEDLTSLEDRNLELGHTIVHPWYAELNPNDKASQDSVSILLRENSLIKVKQYLDDLAKAFEKLPITMNATLCNRGWSYFTDVKSNDPHALIMFWPIHDTLNGELVRLVFFHKYLKWAVTEHLISEAIIHTQARAAGEEILNPRPLELEMPLAHPDIRLLFPGKLPSAPAGGYPLVTAGIHGYPPSKLAARQPAGRVSFQLARYMYLVDRKGLRLSFQPLSMYLASLKETLLASGILWDTRIPARPSAPAGRYGLALAGIRQQIAGICNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCQ1</accession>
    <name>A0A5B0PCQ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015966</name>
        <name evidence="2" type="ORF">PGTUg99_010728</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000345">
        <property type="protein sequence ID" value="KAA1098836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCQ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="62" mass="6687" checksum="39917766FBB68EA2" modified="2019-11-13" version="1">MPSTIEQKSQRMRRVIQPSQAKAEKGVSPMSSINSIGRVLATIISDILLVSLARFAPGAVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGG0</accession>
    <name>A0A5B0PGG0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020489</name>
        <name evidence="2" type="ORF">PGTUg99_031885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGG0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="109" mass="12195" checksum="460FAB4CCCC82340" modified="2019-11-13" version="1">MQRRLSIVENGRILRLLDQEAYPQPFATSVQKLANAVPPSVHHGCTKTVPKRLGGTPGKRSTPSKLKTLPERLDVCAWKTTQRRLAERLGAEALSPPNRLRNVLSRESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PH12</accession>
    <name>A0A5B0PH12_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PH12"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1100915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="116" mass="12847" checksum="1A72072F8114376E" modified="2019-11-13" version="1">MERKSKPLTLPSASSCQLLKANQAGLRLVYTLSKEEASTLLVTYHYSAMPKDVRSLKSGQDQLKPLHALGLPEPTCLTISPPIATPLCVGHRHKESQLDDRKAGVSTWIQFQFSCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJU4</accession>
    <name>A0A5B0PJU4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJU4"/>
    <dbReference type="OrthoDB" id="2506975at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="13486" checksum="A87810E4D94C440A" modified="2019-11-13" version="1">MPEKGLPSLSTKKSSSPQRKNFGMKDLADSLPDSVMSPLRSRATSIALKKLMETQAQMLLDIGAAWKKKYPVGFSYEPRSVANNDGSSVASNHCIRQSSTSQINSLLPANSILPAEFIFLASAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJU6</accession>
    <name>A0A5B0PJU6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJU6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="87" mass="8341" checksum="E687271DC6B192C0" modified="2019-11-13" version="1">MHGPQNGYGGQPGDVYSQKPGYGYGGPPGNGYGGPPGYGYGGPPGYGYGGPPGYGYGGPPGYGYGGPPGYGYGGPTGNGYSGPPGHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLJ6</accession>
    <name>A0A5B0PLJ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLJ6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="12890" checksum="B2DCA1B0499F3044" modified="2019-11-13" version="1">MRYLCMHRISFIVASINKIKDLLTTWFNNVITNKNKSLAQTTSTSFPEETNLANKLTSLDEDNDKRYEQYLKQRKATQSFSTTAELDLYLQEPPVLTDSTKFSVLSWWGTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PN88</accession>
    <name>A0A5B0PN88_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PN88"/>
    <dbReference type="OrthoDB" id="2502487at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="289" mass="32677" checksum="DDD1C776C59DD9FA" modified="2019-11-13" version="1">MQYIFCGTCLIGNNTLKAKFCNSLSAVSDDLFRKSTGQFWLSAENIYRGFDTFHPQLAIPLYKLPLSAFPPNYTEFQEDYYRSYKPTISPRDISEMGMWLGGLSLIPATQFSRYDIKFPPQLLPPPQGTIFMDNSTQPTSFFDAFNTSYHTSCQNQFCSFFRITVNGIGPIVPFGSHDKTLGTIPEALSSMFNHSLSFNVWIMDLAADDLHRKTIGATYLCTKTTKVWLPVLKILSVTMGSSAGVFSAIFSYVIVAFARQYDRRKENQRIDQATQTKIDTLDNQNVDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQR7</accession>
    <name>A0A5B0PQR7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQR7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="67" mass="7794" checksum="FA614F66244362A1" modified="2019-11-13" version="1">MVHTLKSSIKNRAWKWQKAVKTLHKLPKRSEQSGIRTHARRPPDYRRIASRRPGASRRPGELLKTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQW9</accession>
    <name>A0A5B0PQW9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQW9"/>
    <dbReference type="OrthoDB" id="5424058at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="287"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="467" mass="51251" checksum="27EAC8E094B6DAC5" modified="2019-11-13" version="1">MTLSPGSADDRVSGQATHIVSSQKPQSGVAPKGAYYNSSTLSLSFFSPEERTARNHALVDRMPFLYRLICAKINGNYKPNGLDEPADPSGNLERDKLEVSNNEEVNDDDLGDSLDDLVNLDGTEFKRSCDPAIRQTVRAQTIAQMICAMVAFGGNRRHNGLKLSNLQIFLAAGVTERVSSYLNYIGLASSRQTAHAALKTLGTEGAQKLTAQFELSPTLPLMPFLCYDNLDFQEKVHMNSVERTSTMFHGTWGYIHSPPSGVLADLDQAELTIGALNDALHNGSKLIIRPEMFTPSRDSSKHWEKTMKSQITQVILQYIATPADIMVPLQRDPPPVNTIVPEDPQVHVLKLMLASDNSAAGVNDVFTGVIQQSGLTPKEFHSRLHIIEGDLGACNILDSLRRQRVPSIGNQDSLENILPIPGAEHTLWNISQAIFLAHWEARQRHGRMEDPTCFGCASGEAGNQEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSD3</accession>
    <name>A0A5B0PSD3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSD3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023049103" evidence="1">
        <location>
            <begin position="19"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="92" mass="10333" checksum="0495F954B9C1637C" modified="2019-11-13" version="1">MVSIYSLVVAMLLQVILATPSLSTSNEIAFSDSEVLLQPRKQLAKRMSHDVQASYTECAICQNDTEIKNIQHAQIAELLIQCYLKDTGKEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PY37</accession>
    <name>A0A5B0PY37_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methionyl-tRNA formyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FMT1_1</name>
        <name evidence="3" type="synonym">FMT1_3</name>
        <name evidence="2" type="ORF">PGT21_021629</name>
        <name evidence="3" type="ORF">PGTUg99_034353</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PY37"/>
    <dbReference type="OrthoDB" id="10268103at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138:SF5">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="362" mass="39923" checksum="A4366A5D09EAF092" modified="2019-11-13" version="1">MRVLFCGSDSFSSTILNRLIQGNRTLKIFSLIPASSQTGRGLKKVYRPPLLDLVEKLNIPHHSLPRPDNRERNPLKHWSIPDEFIRPEDDEPAPILLSASFPYRIPRETIGRFSHLRALNVHPSLLPRYRGAAPIQWQLAEREPELGVSIQELSPAGFDTGSILAQKPISLPPKTCYPAAETALADLAADMLHQLIPNIDSYIASAWPQDPSKVSYAPKITPLDLRIDPSWSYAKVAGRFRGMGHQHPLSIQLNGEPYQIQVELPDTPVSADFAVQNAARLALTSPGDLLFDRKLDRIALRLGPDPSAPPLLICAAKLLRAGGKGWLGPAAWWDRLFNTAPSVPGSPRSLKTFKVALDPPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q392</accession>
    <name>A0A5B0Q392_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q392"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022956727" evidence="1">
        <location>
            <begin position="19"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10848" checksum="C0C8B9599FE5D858" modified="2019-11-13" version="1">MLIKKSLCFLATPSLIWGIQRGQNPVEPAFAHAYRSTVYLSAPCQVDYTCERQHVHECGATRLYEAQQCSNCHDNEDIQPAGRCNSSHRRCPWPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q714</accession>
    <name>A0A5B0Q714_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_029191</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20689at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09391">
        <property type="entry name" value="LIM1_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09392">
        <property type="entry name" value="LIM2_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000033">
        <property type="entry name" value="Rho GTPase activator (Lrg11)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000058">
        <property type="entry name" value="Rho GTPase activator Lrg11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000219">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF10">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="501"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="567"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="960"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="9">
        <location>
            <begin position="1419"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="673"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="902"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1137"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1293"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1664"/>
            <end position="1858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
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            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
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            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
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            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
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            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="327"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="417"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="442"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="673"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="693"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="703"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="922"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1139"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1206"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1229"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1671"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1702"/>
            <end position="1711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1769"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1802"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1812"/>
            <end position="1840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1841"/>
            <end position="1858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1108927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1858" mass="204815" checksum="44C672E31205306D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9W0</accession>
    <name>A0A5B0Q9W0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHRD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9W0"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022958790" description="CHRD domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="10712" checksum="DC6388031331D83C" modified="2019-11-13" version="1">MNLNSVSGLSLLLLTFACVPAIVRAPTPKPAPKPQPTPACSQASVQITGIYGITGSATFKPTTKSPKGIDVSISVTGLRQQQHTYHIHIRALIKKPTFSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QD11</accession>
    <name>A0A5B0QD11_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QD11"/>
    <dbReference type="OrthoDB" id="2499804at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="158" mass="17838" checksum="32D73F732FAEC841" modified="2019-11-13" version="1">MIPRRPSPGSIEIFFPGDRGPERLNSPVGPSLGNQPQVAPGIVWCQRFPSDWTLPSDKRHINHKPLPPMVRQPSWSGDGVDGGPSSITVLLNWLATNNNYVRWRQGPSKRDLSSEILMEMRRNGIQHRDYHDDITPEVSRPGIKMGKYDGFGHHSRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QI52</accession>
    <name>A0A5B0QI52_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010456</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QI52"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="97" mass="11172" checksum="ADA478A39AA5B479" modified="2019-11-13" version="1">MSTKEKYFRTPIYLKLAIKKPAKIVLGIFFWDTHQEINNGSLLTACFNSRNWIDSWTFSPSPLIKLQVIINTILDMNSPIQLVHWSLKNLTYLSASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QKP2</accession>
    <name>A0A5B0QKP2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QKP2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="144" mass="16307" checksum="68D00D305615FD57" modified="2019-11-13" version="1">MFCLCTAFLPNNFTESTEDSNVKVDKSSCVKRPLAAEAHHRNAFPSDSHRNIFTFATTKITSYSSHDAFGACSIRRVLSIGGWQHRILRERLALPKHAWNEILPLESSQHEQSSYRNLGHHPHLLLWRGRAALTDIGYLCSPHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QM70</accession>
    <name>A0A5B0QM70_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_003804</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2500858at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057402">
        <property type="entry name" value="AIM3_BBC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25459">
        <property type="entry name" value="AIM3_BBC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="4"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="34"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="424"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="789"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="888"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="385"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="513"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="574"/>
            <end position="585"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="594"/>
            <end position="604"/>
        </location>
    </feature>
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        <location>
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            <end position="654"/>
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            <end position="755"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="994"/>
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            <begin position="1031"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1093"/>
            <end position="1104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1114311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1479" mass="158725" checksum="494C22539F62663F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QS17</accession>
    <name>A0A5B0QS17_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008485</name>
        <name evidence="3" type="ORF">PGTUg99_033923</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1074516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2514665at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="13"/>
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            <begin position="36"/>
            <end position="53"/>
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            <begin position="59"/>
            <end position="76"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="98" mass="11679" checksum="9D6931A094AA0FAB" modified="2019-11-13" version="1">MQGTSPLNPFAHPPDQSNSSQQPPPSHQRNWNNNHQNRRGQHHQPYRSQKNPRMHNTFNKNSQRQNSHHNNQQLNRGGNETWFKTSMLQDPWLPLYHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVT8</accession>
    <name>A0A5B0QVT8_PUCGR</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="395" mass="43423" checksum="1A91E9F83C3847AB" modified="2019-11-13" version="1">MVVSFLNNKVFSSFGQYPLTKEFSNQKITPILTISCGLVYAGLLAFNILTQGHGETSESLLLSYPENKIDENGTGTSCGPASLRLGDDVYTVQPGNVENDPEGLGAAQAPGSFQWTIGDISRSSAAAWGKVYTGFLYDGDPMSCNVTSASFTYQFQDTNYKYSLCGLCFLPTYEFRVKLCTTFDLSTTNLPNFAKDLQQSLQSRLGNMDSAYRNLSFEPHTLPLSAYPPYYSQDQIDVYGNRTLTSSDFTQLSIWIGDINFVPFVKNKPLQNTDHSDGTPQGNIFIHRSDKPTDFFSAFKMTGDTSASGFQFSVLGVGGVRPFGSEYNLGSIPASLSDLFQTSLSTLGWMMNAASADLNGRSIGATYLCTITRKKWKPFITLVVKRQWTAVSTRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZC1</accession>
    <name>A0A5B0QZC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Kinase subunit of RNA polymerase II carboxy-terminal domain kinase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CTK1_4</name>
        <name evidence="2" type="ORF">PGT21_032014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2555634at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018800">
        <property type="entry name" value="PRCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13621">
        <property type="entry name" value="PROLINE-RICH PROTEIN PRCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13621:SF2">
        <property type="entry name" value="PROLINE-RICH PROTEIN PRCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10253">
        <property type="entry name" value="PRCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
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        </location>
    </feature>
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            <begin position="286"/>
            <end position="359"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="128"/>
        </location>
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            <end position="154"/>
        </location>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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        </location>
    </feature>
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        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="359" mass="39315" checksum="8DA66B52045DD099" modified="2019-11-13" version="1">MALVDYNSSGDENSEAEEAKIETKTNLPPRKKLKPTKARIIIDLPPTTSKQPELNQNTQPSSIEHQEDHKGDDLLSKLKRKKPASSKSSGLASLLPPPKRTQPQPQPSSSSSSLSFKPKALEKPKHEPTTTAQDQAPSAVQSEPIQATSSTIGTLNLFGLPPISSSDSSASTKRVPVETLEPQISLTVSSAPQVAEEVPPPPSLMDPYPGYWQRKDGSWCERDPSDPLWKAFYLQHYSQPTLETTTTTGSSSSSSDKHSKLPKDFFKDAPNSLVQFDAKQVAQTAWENKPKIIDPREEARQEQQASAATKPSKHISSMARGRHQLSSLLTDAQANRAELEDRISRGKFNRKAGGAKYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R020</accession>
    <name>A0A5B0R020_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">VMA10_1</name>
        <name evidence="7" type="ORF">PGT21_009074</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R020"/>
    <dbReference type="OrthoDB" id="250802at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005124">
        <property type="entry name" value="V-ATPase_G"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01147">
        <property type="entry name" value="V_ATP_synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713:SF11">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03179">
        <property type="entry name" value="V-ATPase_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1118876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="156" mass="18083" checksum="5EF49E6E001F6C36" modified="2019-11-13" version="1">MENTSAELCETHSLASSSPGPSFDDVADYFNPQQQPICWTRKTQEPSSTQRTRKQRIHPYHDRSQKLKDARGEAAKEIETLKSKREDEFKEFEQQHSGNTDSQQKVLEEETKQKIEVIKSEFNKNKAQVVSDLLSKVTDVQPELHRNYKLGIHQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0P0</accession>
    <name>A0A5B0R0P0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008714</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0P0"/>
    <dbReference type="OrthoDB" id="2501243at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1118863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="341" mass="38606" checksum="14958115F805D74B" modified="2019-11-13" version="1">MIPLPNQFHLYTIEEKMNLEEDIEPCLAVKRRHVESLAVKNGDLIEKSLKSISLIRSRLLQQQESNRKKNCEFQQIRNSLEIVHSLVHLVHDDRCNKTWIEFSTDDYKISSNMKNLKLSKVAKEMTFYNEGGETLSIDEVEPKTPTRISVASSYARSESSAGQVRSFKAMSITTNVPTTPTKPTPAPTPTSEIYRLYHSQSSEDLTSAADLTSLEQITPAYHQSKPSPAKAQESYYKTQLSKFNLERSTTSSTDQMVHAEYKNSPSNLSSNSVAVTLTTAHDDEWDQHDDMVNTIESGSTDERSENSSLRKLSIGPNQSRMMALKLKQFTRSERNNLNYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R261</accession>
    <name>A0A5B0R261_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_012688</name>
        <name evidence="2" type="ORF">PGT21_025044</name>
        <name evidence="3" type="ORF">PGT21_028976</name>
        <name evidence="5" type="ORF">PGTUg99_037710</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R261"/>
    <dbReference type="OrthoDB" id="2506480at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1078123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1122501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="700" mass="79970" checksum="94AD576638D84B4A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R375</accession>
    <name>A0A5B0R375_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R375"/>
    <dbReference type="OrthoDB" id="10271583at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="91" mass="10292" checksum="086C5FA9660CF9E3" modified="2019-11-13" version="1">MENSSSLCATLKIWSDHDSAASSSYQKWTTLSCHNIKPEEKLIDCLLVNFITITAQIVRRNFQCKTRILMSSGLEAAEKPRSPQVELSAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3T5</accession>
    <name>A0A5B0R3T5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_031526</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3T5"/>
    <dbReference type="OrthoDB" id="2508778at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="4">
        <location>
            <begin position="200"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1119675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="367" mass="41347" checksum="A43007BE1A4E6FCC" modified="2019-11-13" version="1">MSYSSLPSPKTVVTRSTSIVTRSASAVSSQMFTMDDSTKQLSQNIPGPLTQNKVIPTAVNLSPPDIPLPNPPCSMAKEMKEEDGIVVIRVTPPSEAILNPNDLSWDRFGTLETSYWDRGSDDSDWVDGVRYHLEADGLIMVQRICLYRVDYKKCFKLKPGSKVIAAEILNSGTVFQMVAEYELQKKDLSKESYSIWPNCVMRSYAAARQFLAQFAHAVKSSDDCSTSQRQLAAKLRIVDNFPVHHDFMNRGRSGDPYVEGQDDDMLPAQEDTPESYYVDGPHDPEEEPSRVFIMEEHIEGYSTLLSATTKDYYISNHSKTIDLLLHSFQHWVYAHTKGQMTITNFKGNPPLIAKPQIVDLNKKYGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RAP2</accession>
    <name>A0A5B0RAP2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015064</name>
        <name evidence="3" type="ORF">PGTUg99_029354</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RAP2"/>
    <dbReference type="OrthoDB" id="10685467at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474563" evidence="1">
        <location>
            <begin position="20"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="565" mass="66806" checksum="ADFF6CC803B881B5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RFG2</accession>
    <name>A0A5B0RFG2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023866</name>
        <name evidence="2" type="ORF">PGTUg99_021085</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000208">
        <property type="protein sequence ID" value="KAA1123494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RFG2"/>
    <dbReference type="OrthoDB" id="10285433at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1123494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="405" mass="46149" checksum="0E3F553C2AB1D7AE" modified="2019-11-13" version="1">MEDQSSDTSDEVGPQIRHLGDLVIQAFRNLKHIYESQQGTNGEARLTQAAVTARASPKEIHYDQLHSSLLPLLKELLTTITNLLFPGTLRMEAESRFRKVLELLPELDFQVSQVDSAVRMICPGTSSTESQTDDHDLKQFKRFRLIGLKEMSDQLLSEICVFLHSASEYIETMDLSSGGLFEEYDIENNPQAESEQACYTADHMIKHLNRSELDVAEGCWAGALDVLDELLKNTFSIVTPNAPETSPLRRKLVRQPVIQLTQLTLPIIKLIKLFFKKLSKDGMNHKRRLKLPSFTQMNSHQLDSLSKSAGKIAAELNEIVLLLTQADLAIAEWHRIHFQSIIKIAERLPTHFEPALLSVVLYLIPLIAPHSDQKYYQTWFVTWNILINTALHNFRRLARSLNEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RL30</accession>
    <name>A0A5B0RL30_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase</fullName>
            <ecNumber evidence="6">2.1.3.2</ecNumber>
            <ecNumber evidence="5">3.5.1.2</ecNumber>
            <ecNumber evidence="20">6.3.4.16</ecNumber>
            <ecNumber evidence="4">6.3.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">PGT21_019523</name>
        <name evidence="32" type="ORF">PGTUg99_007678</name>
    </gene>
    <organism evidence="32 34">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33 34" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31">21-0</strain>
            <strain evidence="32 34">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="25">Multifunctional protein that encodes the first 2 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5) and aspartate transcarbamylase (ATCase; EC 2.1.3.2). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the 2nd section; belongs to the CarB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the 3rd section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the C-terminal section; belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the N-terminal section; belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="32">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="6"/>
    <dbReference type="EC" id="3.5.1.2" evidence="5"/>
    <dbReference type="EC" id="6.3.4.16" evidence="20"/>
    <dbReference type="EC" id="6.3.5.5" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000173">
        <property type="protein sequence ID" value="KAA1125992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1924069at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01423">
        <property type="entry name" value="MGS_CPS_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000025">
        <property type="entry name" value="Bifunctional pyrimidine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000005">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000005">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000011">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000036">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF5">
        <property type="entry name" value="CAD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="29">
        <location>
            <begin position="667"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="29">
        <location>
            <begin position="1204"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="30">
        <location>
            <begin position="1461"/>
            <end position="1615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00058513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00081752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="KAA1097778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="EMBL" id="KAA1125992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="2371" mass="260134" checksum="9C31DEF6D2A50B05" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RP98</accession>
    <name>A0A5B0RP98_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018512</name>
        <name evidence="4" type="ORF">PGTUg99_033041</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RP98"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138200" evidence="2">
        <location>
            <begin position="17"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1126945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="116" mass="12870" checksum="B1B5328004039482" modified="2019-11-13" version="1">MLALAFTQLILSRLTGKDDGESAPEPHHSQPDSHSDQPFQWLPNLQCCSIADWPRKEWKIVSKALEARGVCCHSDYNSDCSSESDQGESEDSSDESQEYENSSNEQEGSSDEEGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S2L3</accession>
    <name>A0A5B0S2L3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011435</name>
        <name evidence="4" type="ORF">PGTUg99_037474</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S2L3"/>
    <dbReference type="OrthoDB" id="120613at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052282">
        <property type="entry name" value="Starch-active_LPMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36575">
        <property type="entry name" value="BINDING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14430)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36575:SF2">
        <property type="entry name" value="CHITIN-BINDING TYPE-4 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1132147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="157" mass="17543" checksum="2F9638A8E91E8964" modified="2019-11-13" version="1">MQLGNEFSSKCGLGATAVIKKDNFISQELWKKQASPQCKARRCQGLFLKDQDLTKVTNFPPGKDIPVTIKIVVPHVGKAQLFLIDMQGKKQDLFLMDLGDQFGKGKAFNSFQDFQVKIPSEKSKDCVQPGKCAIAFRWTVIPLKETYDICSLITIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S4B2</accession>
    <name>A0A5B0S4B2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018461</name>
        <name evidence="3" type="ORF">PGTUg99_034228</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S4B2"/>
    <dbReference type="OrthoDB" id="2499165at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="323" mass="36525" checksum="85C09377288AEA95" modified="2019-11-13" version="1">MMAHSAVQSPTLGTNSGAGNSTRRLRTRAPRSNLLAQISFDDMEYFTHPKNRPRTERRSNRSPSKAMSPDEDYEDHSMSIDPFIHHPASKSTRRLKISRQHRISPPPPLPSPQLPYIQSEKYPAWLLGDAMAKSSANYLSQKTQEAPLTTPLSSQQQNTQTSPIRRSASSFLGTSPLKHHHFHTSLSRQSSSNNLNFNNKRPSLGRSISPDEALDRRREKFWELYEYDSTSPHLQARYFDKPLPTNEQWADLDIQNVAIIPDPQPGPPAVHLVEVFQPTRVDSTNPQPPSNSLSHIQLNSQQLKPHIPFGLSLLPSDYALHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SBC7</accession>
    <name>A0A5B0SBC7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020108</name>
        <name evidence="2" type="ORF">PGTUg99_013874</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000041">
        <property type="protein sequence ID" value="KAA1135112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SBC7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1135112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="83" mass="9315" checksum="502725B78A62B19E" modified="2019-11-13" version="1">MINFTSHHVRLSLSLKENTTYLLALRSDQHTTTSTAWADSTVRGPCIALRRAPWYVGWAPEAPELMAGVPQAKKTRPSCYLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SDY4</accession>
    <name>A0A5B0SDY4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011890</name>
        <name evidence="2" type="ORF">PGT21_012703</name>
        <name evidence="4" type="ORF">PGTUg99_017783</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1135992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SDY4"/>
    <dbReference type="OrthoDB" id="2508453at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138343" description="RanBP2-type domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14599" checksum="666B5C059A0A101D" modified="2019-11-13" version="1">MQLHTGRAALICAVFVSAMTMCSSPPRMPARLPRPLRRGNSNRICANCVTEGYEVIDGQRCLYELPSPARRPCQEILEPIRGEPPCPGIITGKEWRCDKCNCRLFESAQGCLTCESGKAKRYFPQSRAPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SER1</accession>
    <name>A0A5B0SER1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012846</name>
        <name evidence="3" type="ORF">PGTUg99_022435</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SER1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1136025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="60" mass="6714" checksum="456C96C086C88F6A" modified="2019-11-13" version="1">MPFKTRAYRKVPPRRSQRKEAILAEPSDREAFVKLGQDRSSSGGTDSMDDRSGCAPGTEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJH4</accession>
    <name>A0A5B0LJH4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_004268</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJH4"/>
    <dbReference type="OrthoDB" id="2125469at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10951">
        <property type="entry name" value="CE4_ClCDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471:SF2">
        <property type="entry name" value="CHITIN DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023008707" description="NodB homology domain-containing protein" evidence="10">
        <location>
            <begin position="31"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="11">
        <location>
            <begin position="90"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1064475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="313" mass="35072" checksum="DA05D0CF82B3864A" modified="2019-11-13" version="1">MPNSATGKISTTKMNLWILLSALILLPAAAFTNSVVTLPSHNGNQPENQQQQRQADDNHVPATNHSRTVGKHGAHAQSAFKVYNTCKLSKSFSLTFDDGPTEYSAKLDKTLEKANLRGTFFINGNNFGCIYDHSEVLVERFKKGHLIGSHTWSHVHLTEGTYEQINHQIELIERAMIKILGVKPLYFRPPYGEYNDDVIKVLKKRGYKGLILWSEDSQDSLTSPPNPHEMIKTYQTYSDKTIVLSHETHPFMIDEVVPGVIPKLKSKGFKLLPVADCLELGSTPNDWYEVVKMPGSKDESWTCDGTPAPGMFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2024-03-27" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQ23</accession>
    <name>A0A5B0LQ23_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQ23"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="159" mass="17848" checksum="C1E6A5815165237C" modified="2019-11-13" version="1">MFTIYLAPENSESTLTEDHISFCGVQAKNGSFQFKEEGPKWNSKFAGVELEDSIPYLIIVFSFKTTSTNYKLPEIPQRGSLIFHGLDNIDCLTPEISIALKDLLSVDPDIREFHKDTKHIRRYVETTRPGAYRFQDPGVNSFNADEGIVIGGAPDNQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRG2</accession>
    <name>A0A5B0LRG2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024095</name>
        <name evidence="3" type="ORF">PGTUg99_010641</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRG2"/>
    <dbReference type="OrthoDB" id="2510033at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="333" mass="36195" checksum="0053A152234DE20C" modified="2019-11-13" version="1">MPQNPTTPSLEASERYPDTLCGSKRVDHLPSTSGFSTLPLESPHTALPSPIDTRYCFESSSSISPSSASSSTWTSPSTNPFWSPPKSEKSPSASSVIATNLADLIDDLFIYSISQEDLSGFLNVAPKNHSQSETSHLTSFFAPSNTADSAKSIDVQSLARENPITRCLSDLHLLQTPLRSAFQEVFDSLSDDAPEPVAETYQCDDETVNCSQTPSDVSLTQSDVNSLVISNSQSLEETRLSTSPCSLKRCPARVKRSSRERSWAPPTIDLPPRPKALTQPKALTRPKALTKPTAPLSGTFVFDRAFIRQTRHFKYWTAILDAPPSVASSSRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWU3</accession>
    <name>A0A5B0LWU3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM complex subunit LSM3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LSM3_2</name>
        <name evidence="9" type="synonym">LSM3</name>
        <name evidence="12" type="synonym">LSM3_1</name>
        <name evidence="11" type="ORF">PGT21_007227</name>
        <name evidence="12" type="ORF">PGTUg99_008316</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000441">
        <property type="protein sequence ID" value="KAA1081508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWU3"/>
    <dbReference type="OMA" id="FDSHCNI"/>
    <dbReference type="OrthoDB" id="29543at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01730">
        <property type="entry name" value="LSm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000036">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034105">
        <property type="entry name" value="Lsm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040002">
        <property type="entry name" value="Sm-like_LSM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13110">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="11"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1068962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1081508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="97" mass="10655" checksum="26A5977F14F63B5F" modified="2019-11-13" version="1">MADLSGDGVEEPFDLIRLSLSERVLIKLRGDRVLNGTLHAYDGHMNMVLSDVIETITVVEPAANPDDEPIIRTVKRNCEMLFVRGDGVVLVAPPART</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYC0</accession>
    <name>A0A5B0LYC0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Crossover junction endonuclease mus81</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MUS81_4</name>
        <name evidence="2" type="ORF">PGT21_009247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYC0"/>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="140" mass="15445" checksum="3EF1158E65E2074B" modified="2019-11-13" version="1">MQNQNGEQPVQLGPGDEELHCAALKNDLLMNGNPRLSESPEGSIGPWKAFAQTSFESGQSNPSGRRSSDDIQPEVRKLGSDFDICVVLDHREKISSPGRYLIRVLQRAPVVGNAIWIAVHRDDQHEFFSGLRADPISLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYK3</accession>
    <name>A0A5B0LYK3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028489</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYK3"/>
    <dbReference type="OrthoDB" id="2506706at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009816">
        <property type="entry name" value="SPATS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15623:SF11">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED SERINE-RICH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15623">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED SERINE-RICH PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022800843" evidence="2">
        <location>
            <begin position="17"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="752"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="990"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="560"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="799"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="843"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1039"/>
            <end position="1103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1109" mass="114926" checksum="16E58D80200DB5EB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZ91</accession>
    <name>A0A5B0LZ91_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZ91"/>
    <dbReference type="OrthoDB" id="10415470at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="97" mass="11132" checksum="BB01376CCA85629F" modified="2019-11-13" version="1">MINLHRLARIAKPNATNLPSFWDEIDEDLKERRKNTSLRNNVFGQLILDKDAQLWDGEKTIHDWTESDQQLPSDEEVQARVELETDGRPAGPAVPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1K5</accession>
    <name>A0A5B0M1K5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_001635</name>
        <name evidence="3" type="ORF">PGTUg99_008049</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000486">
        <property type="protein sequence ID" value="KAA1070063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1K5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137137" evidence="2">
        <location>
            <begin position="27"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1071263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="159" mass="17899" checksum="6EAC3E0F22D35390" modified="2019-11-13" version="1">MKAFAFLSGLDQLLLAFWSAAVRSLALKISPFEPEGIGTGANQRILILKIVPLPARDIQPISSFRICMSTGYDREVPIRFPTVMEADDCGIWDRHKFVDQKIKLLEFDFGSTVGKGYLFYLSVSHFRAPERNAKPDRSIQSIVPFPPATQERHDGSHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2T2</accession>
    <name>A0A5B0M2T2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2T2"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="436" mass="50223" checksum="1EF3305C3D94FEEF" modified="2019-11-13" version="1">MGQDYSALFNQPMIQQEWAISVKPVVLPVLTSIFLLFISAVSLPCKGRSAVYFRLALLPFQLILTHDICVNKKYSLGSAFRDSSFPTFGFLLLYRALDFSILNLWDPEPAFHWIVPDRPPTTESKSESPDEKPIRWRKVPHPPLFSFDRMLWALDNLTLMRPGTPFLFPWKIRALEWSQYALESPQTKFGEPEWPLLPAIIQQLVHLGCHLYVRQLDLKAGQTIWTLPLFDQLALTFALGGAVTFSYALEEAIFFPLILRFNLLPKTALISHSKRSIASAGLGELWGKRWHHVWKRCFVRLPRLMPGSSHRLVQMFGAFWTSAMFHCLVLGRLYPTPDISRPISFLPLIYEPGIYKFFLCQGAGVIIEKILLGDPKKDERLTKRIIRIFYLYFFLLGAGRYSTNSLAVKGLLDRDQWNTVTIPAISRMFYDIFFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M367</accession>
    <name>A0A5B0M367_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Inosine-5'-monophosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">IMD1_2</name>
        <name evidence="14" type="ORF">PGT21_012102</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M367"/>
    <dbReference type="OrthoDB" id="416622at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000424">
        <property type="entry name" value="Inosine-5'-monophosphate dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF111">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="13">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="242"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1070430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="361" mass="38036" checksum="9AC4854DB41F3762" modified="2019-11-13" version="1">MTKLSELVVGPEGITLEEANAILRDCKKGKLPLVDSEGRLRSLLARSDLLKNKDYPLASKKPASKQLLCGAAVGTRETDRARLTALVQAGLDVVVLDSSQGNSIYQIEMIKWIKETHPGLDVIAGNVVTREQAAALIAVGADALRVGMGSGSICITQEVCAVGRPQGSAVFAVAEFARKFGVPVIADGGISNVGHIGKAIALGASAVMMGGLLAGTTEAPGEYFYNEGQRLKKYRGMGSLDAMEHGNAEKNPVKRTASPSLQTRLKTGASQSSEDNAATSRYFSESGSVRVAQGVTGAVVDKGSLKKFVPYLYTGLQHSLQDVGVRSIAQLRERADGSEVRFELRTTSAQVEGGVHGLHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M789</accession>
    <name>A0A5B0M789_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022398</name>
        <name evidence="1" type="ORF">PGTUg99_013218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M789"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="68" mass="7649" checksum="BAE248121473B99E" modified="2019-11-13" version="1">METAEQPHLCMYIQPALTPLSHSPHYKTIHHPSPGLLHCQINHNSQLFHSLLGILNYAAVFQTAKPHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M871</accession>
    <name>A0A5B0M871_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-oxoisovalerate dehydrogenase subunit alpha</fullName>
            <ecNumber evidence="2">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_029183</name>
        <name evidence="5" type="ORF">PGTUg99_023800</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BCKDHA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M871"/>
    <dbReference type="OrthoDB" id="3845at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000108">
        <property type="entry name" value="2-oxoisovalerate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="3">
        <location>
            <begin position="96"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU365014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1072168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1135268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="445" mass="50208" checksum="08B304D266B3678B" modified="2019-11-13" version="1">MMTRLMRMNELGRRTWKSTRMARLSQPTTTTTTITKSFSSHSISKSQEQQPLPFDPLIPLKILNPIGKKNSNDQHIGLPDLAQLENESFAVKVYEVMLGLPILDTFMSNLQRHGRISFYMTSYGEEGAVVGSAAALGDHDEVFAQYREQGILLWRGCSLDYLTAQCFGSVEDESSKGRQMPVHYASKKHHFHSISSPLATQIPQAAGAAYALKRMRQKGERPDDCVVCYLGEGAASEGDFHAGVNMASVLGGPIVFFIRNNGFAISTPSSQQFKGDGIASRAAGYGIDAIRVDGNDPLAVYLATREARRRALEGAGRAVMVEAMTYRVGHHSTSDDSSAYRNPNEVDQWRKRDNPINRMRAFLESKGWWDPAKEEDRIKQWKNDISKSVKRTEKMSKPSLKDMWTDTYGTEQNHLDQQRIESERIIKKYSEYRVYKEELKKFTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MA24</accession>
    <name>A0A5B0MA24_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_002216</name>
        <name evidence="3" type="ORF">PGT21_002259</name>
        <name evidence="5" type="ORF">PGTUg99_028013</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. Sedlin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MA24"/>
    <dbReference type="OrthoDB" id="18320at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14854">
        <property type="entry name" value="TRAPPC2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044760">
        <property type="entry name" value="TRAPPC2L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1076311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1135642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="155" mass="17473" checksum="34DB54DEC498F1A9" modified="2019-11-13" version="1">MSSNTGRLPILALAIIGKKGQPLYVRNFASPSGGEADLKWHYAAHVSLDVFDERDALPTTLVDCYFGLLYAMEDYACYGYQSNTRIRFVLCLPMKDTLIKDTEVKLLFKSLHAAYIQHICNPFHQIPIDNPNPNPITPTIKSKTFDQQINRIVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB93</accession>
    <name>A0A5B0MB93_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB93"/>
    <dbReference type="OrthoDB" id="2506010at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="308" mass="34697" checksum="55CEA5D036AA9793" modified="2019-11-13" version="1">MSLPYTVPDLEDGFAPTTPLGNRPFASGSNSNWNTGFTAPPPPMKKDKGKMPANQYREPQAEEEMSDSEDPTPQRQAKQTEPPVTGYGLHNARGSDGEPAERRYGPRIIKEPGLVYDGTNFTRFLAIYERAALAFQASDYEKALQIGRFVHKEELKLELEAMEGYNSFNWTELRKAMKESWGELDNTILYTTADLVKIVEEYSRKGGLKDYKEYKAYLGKFTTILKYLVDNEHLNKKQDASLLFISAFSKESQKNIKRTLVSNGQLPKGPDGSSKPPLWKHVTEAADTEIRVEEEGYFAVSGFSEANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBM0</accession>
    <name>A0A5B0MBM0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBM0"/>
    <dbReference type="OrthoDB" id="4092340at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022683575" evidence="2">
        <location>
            <begin position="23"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="273"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="432" mass="45716" checksum="6B4BF97A9627D393" modified="2019-11-13" version="1">MRSTCPILVDLFSAWLAHSTLAHSLLQEQIMIGQVHAHQAHAQAQAEQQHQLHNTFYSTNKAPAPGRMTFTQGQGGMLAERALARRQQAEADALYAQCYPNVEEEDNVLRELGHGPSFSSVSSERSSNMTPPAVVVSKPDEAFPLLASADSLFGKAPGYPKLTNQQASADSQGVPSVVTPSQGSVSPSSLTDNSQAKDSPVAPVKKKFSDVVKSSAVKPAPAAPATSNVTAVPPPKLNPFSSVFVPGSNNSPESEAPAVFTKASPTQKVVSFTEPTPEATSAAAVYRAPRAKSPPSAKGNRVSSTPLPAPSIQSVPGGMPTRQPRGPAAEAELTNQNFATRIRKRAIGAIKTLGTRPPTSPSPVPAASANNYHYYQQQQMIEALMMQQHQQQALAAAGYVDSDLGFHSAMHSSGGIAHLMATARAREERFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBR4</accession>
    <name>A0A5B0MBR4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBR4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="170" mass="18892" checksum="C7CD6317708391F2" modified="2019-11-13" version="1">MAERNPLGRRDSALSGRGDIPRPSAVESIHLGHRECTLLMAETLRVVSQLLALSLDHWDCLQNFGITVDLKSKIITILLVPSSTEPRDWTLDFQDYYWTLETVAGRSTSLLDSRNCCWTPEIVSGRSESPLDSGYCLWTFGIASGPLGLPWTFGITSEPLQPSLDLSDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEQ1</accession>
    <name>A0A5B0MEQ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEQ1"/>
    <dbReference type="OrthoDB" id="2507030at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="419" mass="48461" checksum="07B18E7232ABFCCE" modified="2019-11-13" version="1">MDKLEEICPKCFGPPVPRKTPEEPDYIVCMDGNFQHRQHLAASHELSEYSNQTSLFISPEEVEDMESCMNIQINEAEEHPNLDRCTKQHTAANNSRGSTTWKACDDTGIFGLEFKRLLDRGLHVKKLLVESMNKLTKIREKHNFTIEYLTEQWIRQRQCQLEAMDTESEREMIKLVGDLVNLEDELQDAQDDIELLRAKRRGTRTVEEQERLELLPNTVTSLEEQIEILVDELGSEAFWNLPGASDAQSKALIRLKTSKSKLYKAKVGVCEVQRRWDQRGSGTRMQARFKKLMSSKMKHLKSKWTLYNQKALNYNENHSTNILVATPVFEDVRSMGLDDPFWNIGSLSHPNEPWAIDSTIKEGIEAILMSTHCNEDLRRISREARQAIKWAVEKSKCLDMISKLLHRGQLAELWFLLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MES9</accession>
    <name>A0A5B0MES9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MES9"/>
    <dbReference type="OrthoDB" id="2499245at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="257" mass="28331" checksum="A3761BDAAB93526C" modified="2019-11-13" version="1">MDQVTQPRSARAGSHILAPNLATFFAPPKIAYQRLRAALGQAWGIRRLANVKNEWVQQANDDNNGLYTAYYHFCQSYDNLGASLNDPAGDGASRSAMELVQNFCGAMAGLAFICTKNNMKAKQAKLDKLRTVHHVQHVQMLANLLIFGPTAAFTSLHKGPHMTLSNANALLELGSSVLKQKIALGIMTPMPHLTLDNLRIHFLNFLIKMGFNQLDNDGVPQVNYKDVHASFYNDFSPSVIAGIFSQDIEIALAHRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFI3</accession>
    <name>A0A5B0MFI3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFI3"/>
    <dbReference type="OrthoDB" id="10411325at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="135" mass="15145" checksum="9C6A5F6A08801DC1" modified="2019-11-13" version="1">MVGAELGPPHVLQMLHSQGDISTKWTTAQHQCQLAIAPRKSKAVDLRARLFDIHIMATKNTLTLLNMMIPIRKRHRPQPTFVARSLASTTQKELFIMAESNVVASPGSIPSALPKSKAVIVIHSRRHSWKRKLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGA1</accession>
    <name>A0A5B0MGA1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGA1"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026894">
        <property type="entry name" value="DnaJ_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44924">
        <property type="entry name" value="DNAJ SUBFAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44924:SF1">
        <property type="entry name" value="DNAJ SUBFAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="87"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="498" mass="54371" checksum="041F5D5B2DFE892A" modified="2019-11-13" version="1">MSPTIIPSRPREAYVAITCAHCKSHLEYLPPPSSHNSSKDSYLIQCHSCSKTFLPNSSAGTTTSSTKPSTKKTARRIGSDERPLETEYYDILGLTPKATALEIKAAYRRMALKMHPDKNPDDPDAGEKFKSLAVAYNTLSDPQLRKKYNEFGKQQENGGGFVDPEAVFSTLFGGEKFQDIIGTISLGQEMKTAMQKESNEDEEQENDTGSQLVSASQQPPATSSSPKATTKPTLTPEQKAKQDAVAQAEAEERKRVRDARVTKLAEKLKSKLYLYTEQAEEEFDQQVMDSVKMMWEIEKESLAEESFGPELLRTVGSTYLAKSKRCLTATATGAWGGGVALVGGWFHSAKSTAHVLSETVGAVRAAYDVKAVFDELAKAEAEGGPGLSEERKKELEELAAKKGLRALFMGAKLEVESVIREVCDRILEEPGVSREVIRKRAVALGILGSVFETAKNKNGEDTLAELENGYVKVDPSKKKPATSPTDQQDSKAANATSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGZ2</accession>
    <name>A0A5B0MGZ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034627</name>
        <name evidence="3" type="ORF">PGTUg99_012157</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000179">
        <property type="protein sequence ID" value="KAA1125355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGZ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="120" mass="13712" checksum="2B3DABD97903E2B9" modified="2019-11-13" version="1">MVIQLCKGLWPVSPRFLVKHSGARRFETTGGDAPLNHTNTDTQPKIEPLGHSPPAVETSCRSKDQRRPPNNAKPIDRTIVFGIDTRKEIRLDIQIRDRLSHPTSNHYKKAQYSHRFFAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJL5</accession>
    <name>A0A5B0MJL5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_005231</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJL5"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000674">
        <property type="entry name" value="Chromosome 19, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.60:FF:000006">
        <property type="entry name" value="Predicted mechanosensitive ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058650">
        <property type="entry name" value="Msy1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323:SF11">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25886">
        <property type="entry name" value="Msy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="566"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="591"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="513"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="21"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="115"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1077377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="722" mass="82048" checksum="D909111F161D17A1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MK16</accession>
    <name>A0A5B0MK16_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MK16"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
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        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="332" mass="35653" checksum="DFD750F47120EF11" modified="2019-11-13" version="1">MHGDNPAANAYGHMELGGDLGSLIADLGPGQPNDVGPASERPPEDEEAQRLRDRRGIAGLTAEELALDCLDEEDLGCSPSGVLTNQTHPTTGGSPGRVRTSGAALFDGIPRTPSVAMGSGGSGSTTGPAHPSTPTVAPTPAVPPTPTVPPTATVTPTPTVPTDVIPMAKPPPAGPHPARRRGRTEVTKHEDSSAGALLVMLQKSHDWQDQLRLEDRQVAEKKAEAKDLVRLEAMAEAAHDRTIRDGQLKIDRDLAEKWNQLMDNKFRAREADRKEERRLDKEWRAAEGRRYEASQKQLAANRKDQDDARRAQEQSTQLIQAALMRMLGVGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQ21</accession>
    <name>A0A5B0MQ21_PUCGR</name>
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        <recommendedName>
            <fullName evidence="4">FAR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_035725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQ21"/>
    <dbReference type="OrthoDB" id="10538451at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="41081" checksum="8ABEB5A57D49A241" modified="2019-11-13" version="1">MKRSNSQHPAAYERSNSDQRNLVDPAGDWENACWDLGDPTANFQEVRSSFVPPVPEDISPLPLAPVDALLSAPAGALALDNCLSSNMQAPDVAAHPEQHVTMGIKTNHLDPGTASPGRPDIPLVDSTLTGLPDLDVAGENLTVVKAGKSISDPPMEASSSAVRLENDAGIASDGVLTSADVNNFDFTVGNPMDRPPLLKFHKIKDLRRFVQRWAVTHGYKLPTGNSGGNRNVYLRCSLAGEDSKKPQSDRQRMSRNRRIGCPFRVSGHKPTAEGCQNFPWEIRIKDHLHHNHGPMDVEYQVLHRQMDPALADEAVRMASLGMKPQAVAVMVSDKAERFVTNRTIYNATAAHQQAKRKGKSVLMYSYNEKACGLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQC0</accession>
    <name>A0A5B0MQC0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQC0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="61" mass="6333" checksum="7EC28F2DEF208DE7" modified="2019-11-13" version="1">MPRYAVSPEIASLSLQAQWLSLTVLFCGAAAAVVDVAALSTSGRVWAHRCSPSCHAGFGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQF9</accession>
    <name>A0A5B0MQF9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SNARE-binding exocyst subunit S6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SEC6_2</name>
        <name evidence="5" type="ORF">PGT21_002626</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQF9"/>
    <dbReference type="OMA" id="MNIGPKT"/>
    <dbReference type="OrthoDB" id="190098at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000006">
        <property type="entry name" value="Exocyst complex component sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="43"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1079175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="773" mass="87975" checksum="23D3854DF3DB2297" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUY2</accession>
    <name>A0A5B0MUY2_PUCGR</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019158</name>
        <name evidence="2" type="ORF">PGTUg99_019149</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
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    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
            <dbReference type="EMBL" id="KAA1092100.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="179" mass="20161" checksum="F66D9AEE53554ED4" modified="2019-11-13" version="1">MDWVFKWYGGVPRHVLELATTAFKEHHGDEDKALKALVQGMNQAIHKVTPVESLDNFTWHGHCPNNYCGLVFEMHTQSLLQEGGRFQVRQLGKGDPQTGQSITITFSEAGCQTFKTYKEVDLRTNVFWKPCSPNLASIDLLRGPANVFQITVSNTHPIKHEGLNKALNLVEKSESSQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0MUZ3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">PGT21_007217</name>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
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    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="496" mass="56147" checksum="428D0611A68E2CFC" modified="2019-11-13" version="1">MFDTPADNLTPESVMKDQPSLLRNEKIWSPAPSGVNPFAYLMNRSASVGSAPMSGLTKALLIVFFIIHLIIAIFCLVILVLPCLRGSRRSHWLFRRLYIRDQSSVANVSRAPLFWLNGGIVMTASQLMGSLATGAYILVQFMITHSAKFALHAQVEPALAMMCVCEMLTYWSQMHCFVVAIYYNHRAVANRARRWTPSPTFINFLFLGFPIILVITALAPVIGLSLIHRQLTARVLQTFDTLMSGSLIWEQMRVSTSEKEKYQLTAQLTQVVSQAKTLGDEVGIRVERLKTCFYSFLWDMLAQLCITIMLFFFVFSIFLHKVQQKEGQPENDSHKSSSFRRWFKKDLPPTSETKPMDPPNENQEQFSNTSRVLINRQLAYLVLRALFIIIAIATSITLCILGILRTHDGTSQSPYWRAVMTWLPPLAGTWSAFPIAWQCWGLYNDELGGSPRTSANSKNAPSAGETIQKNTIQPEHWECELEEIEIRASPMIINPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYX4</accession>
    <name>A0A5B0MYX4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_034392</name>
        <name evidence="3" type="ORF">PGTUg99_014607</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1092018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1735"/>
            <end position="1745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1820"/>
            <end position="1831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1892"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1921"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1943"/>
            <end position="1964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1983"/>
            <end position="2013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2028"/>
            <end position="2037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="2037" mass="214981" checksum="F7D78C8EC2472986" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N153</accession>
    <name>A0A5B0N153_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N153"/>
    <dbReference type="OrthoDB" id="2564904at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022906795" evidence="2">
        <location>
            <begin position="20"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1082532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="220" mass="23253" checksum="6E91B5B75DBE1C6A" modified="2019-11-13" version="1">MFQPQLLVLFTCLVALSKQQAPPSAPSPAPVPPIGGSGPPPAPITTAQVGPWKPPSGSGGLPPPSLNGMGRQIRINSATDFCILMPPDPTKQNLVDAEANAVAYCTNPVNDTRPMPDGFIKTSHFRHTDKYVQISGTYDPSKMNLSPNDCGGEYDNHGAMGVGNPVGAAVDGAHDFMQFMGGCDIPGNAVFCLRACHGDDSYEYCKNTYDLMGCLWVMPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7K3</accession>
    <name>A0A5B0N7K3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6818 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028738</name>
        <name evidence="4" type="ORF">PGTUg99_022671</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1133017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7K3"/>
    <dbReference type="OrthoDB" id="2499380at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049203">
        <property type="entry name" value="DUF6818"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409:SF1">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20681">
        <property type="entry name" value="DUF6818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6818" evidence="2">
        <location>
            <begin position="121"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1133017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="340" mass="37470" checksum="49C8A4B595225223" modified="2019-11-13" version="1">MLILASREVYNDLDPPPWSYRHHQLTAPFNCFSALCLHTIQLTFFCIASIMSQMCTGHLSTQPHTQPITQLTQPITQTTEVPSTNAPTQTTGRRTGRQRGSQGYSGNDCLALVNFVKHVLPLGSNDWDRVHELYDQYALEAGRVSRDPDPLKTKFRAMVASKKPTGNPDCPVWIREAKRANIMIKDRACSIAFVDEDDGEEESDDERNGVGNPISLLPGDPGYDNGATQTQGTLLSGWSATQSQLPDNPQDIVEDPNDESLAEYADFSQSSTLMHHSTMANRASSTAANTSRPGPINTQLANDYQVRSRDGRSASGYPSGCGYPMAISAKLHIRIRTRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NA20</accession>
    <name>A0A5B0NA20_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_009011</name>
        <name evidence="8" type="ORF">PGTUg99_015215</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000213">
        <property type="protein sequence ID" value="KAA1122991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NA20"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051732">
        <property type="entry name" value="USF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117:SF3">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="322"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="123"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="246"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="421"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="171"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1085493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1122991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="583" mass="65142" checksum="8F6E537911B52A5D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND99</accession>
    <name>A0A5B0ND99_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND99"/>
    <dbReference type="OrthoDB" id="367221at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="176" mass="19506" checksum="D71B969611393B6C" modified="2019-11-13" version="1">MTCSANSPSEFEKIVTNLLRPPFGLKATPDLKKNDAEAKDGAPPEAASVVCPREGLKFRIEPIIRCHDKPLNRLEVIRITGNVVKLFNGNEYIAMADECSVAHQASNLLHQISLSSTTHPAHHSHRIAIGAHPDLASSIAKWLLMSVDIDVPTTPFFHPDKHPPQNLEARFETFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NE01</accession>
    <name>A0A5B0NE01_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Proteasome subunit alpha type-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PSMA6_3</name>
        <name evidence="2" type="ORF">PGT21_031747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NE01"/>
    <dbReference type="OrthoDB" id="2509292at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="174" mass="19734" checksum="0BFE541F4E5C02F0" modified="2019-11-13" version="1">MSNQHRGAKPSDAENSQAHPTTHKSRIVQKPHLTLSPVILQIDSKSTPDKPFLIAKPTGPLPMSGEADHSGPPADHPFAGGERPRKRRRSVKLALATVVEHPELVRFRRKIREIVSSSSLRGPRQYYASKRRRPLRLSPNRPLKKDPNRQWMKDVLREVDTLISESVLMEIVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NG21</accession>
    <name>A0A5B0NG21_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein M3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ECM3_1</name>
        <name evidence="7" type="ORF">PGT21_011634</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NG21"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040254">
        <property type="entry name" value="Ecm3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31274:SF1">
        <property type="entry name" value="AGL149CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31274">
        <property type="entry name" value="PROTEIN ECM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1086779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="441" mass="47830" checksum="02E276A10817F639" modified="2019-11-13" version="1">MSDLSLSQLVWISIKPLIKVLLPTAVGAAMVRYRKLDQDGLKAAAHIQIYGALPCLMFSNVVPSITAQNSPRILVCVGFGLLYMLMSYLLSKALLMVVPVPNNFRNGFIVAAVWSNWGNIPMSVIQSLTGGPPFGKPEDVEMGVSYASFFVMVYNVMMFVGPGTKMIDRDYMKTEEAAITTPYLPYDPTVNQTERPSIDDQPGQNGEANRPMRASPEADIRADQQFSSRQSRGQKGVLSGLLNNLSPVIVSLLFGTLVAVTPPLKGLFTKLKDHAVQEPSAPDGKPLLSVILDSTEYLGAAAIPLGLLVTGASFANMSIPRRSWFRLPLRAIFGLTLIKLVCLPALGIVAVSFIDGYTNFFAGEEGRVLKLISLYYSCTVTSTNQISLSSIAAGIYIKPGESSDEEVASNVDLLCSFVALQYLIYPFVGTFFIGLIVKMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NH30</accession>
    <name>A0A5B0NH30_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003313</name>
        <name evidence="2" type="ORF">PGTUg99_009285</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000405">
        <property type="protein sequence ID" value="KAA1088625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NH30"/>
    <dbReference type="OrthoDB" id="2506076at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="190" mass="20672" checksum="B7488DAE47D1D4DA" modified="2019-11-13" version="1">MNNFIDEQNMGWVAAPVPVSSQYHGVLLAPYGPNPEAVEAYQISGCAIPEPVFRKTSIAGNFGNRSTMYTLPSQALHSTCTRPASVVTRSNTTASNQETKLESLVRGTSLACSEQQSTVIEGPPQGMIDQSANLRATLRDAIHRKKDSDEELLDSLMLLWHDRNSEAPTGPARLQTPEISPPDHIETAKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI46</accession>
    <name>A0A5B0NI46_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="14">3.2.1.58</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Exo-1,3-beta-glucanase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PGT21_033305</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Glucosidase involved in the degradation of cellulosic biomass. Active on lichenan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="14"/>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI46"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000033">
        <property type="entry name" value="Glucan 1,3-beta-glucosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF34">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="18">
        <location>
            <begin position="287"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="74"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1087539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="765" mass="81941" checksum="07AF05CA13CB7710" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMJ0</accession>
    <name>A0A5B0NMJ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMJ0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="14205" checksum="246221EED473955E" modified="2019-11-13" version="1">MDDSSAPEMTQVVELLPRYESAADPKGSRRGRRPKLYKDLSLTVESKILTLRELFLRVYDLEGVVTHPRIWTLQSQSNSTPQAILGTPPVPRPSSHGRLRHRLSRDLQQDIDDLVLSFSLLNLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTD1</accession>
    <name>A0A5B0NTD1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTD1"/>
    <dbReference type="OrthoDB" id="2509176at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="479" mass="51235" checksum="F3130587A4092339" modified="2019-11-13" version="1">MLAQDALPPRFEDLERCLIQIEAALTRRAAAEQPPDVNTSEDVAATSTSPAVTRMQQRLSDALAPRQRPPILLTGINSDPIHTADPPPREPLACSSELELDPQPGSSEPLLHNPPHQSTTGSLQRETLSTRVKEVISQILDCVEPRGRNPHPDPWVNALSMESGSMQVNPKPASYHAANNSPRHMSITTSSKPTFLNRLDMFFFNFFTRSRSIPAPKQPGILVGPSSGRISNVIASSTPNPLGASAKGKGQPIAKVTVPPNNPTEIYSGSLKVDPDPVPSIHSVPVSAASSSPSSMQIEEPGESTLNDSDEITFLGINLSPDKLPLTHNRRSCCAASLSLQLSPFVDCVSISSSSKSKEHNFIFAEQTVPSMNPLVIQSGSLKEDPDPVPSNHSIPVAAASSDPSPMQIEQPGKSALKESEKITFLGINLPPGSFPLAHKTRSRCFASVPPQALPCASSSKRSSSSEAGEGNSYKRLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVI1</accession>
    <name>A0A5B0NVI1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase mitochondrial F1 complex assembly factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_028507</name>
        <name evidence="7" type="ORF">PGTUg99_007908</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000146">
        <property type="protein sequence ID" value="KAA1127957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVI1"/>
    <dbReference type="OrthoDB" id="16535at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010591">
        <property type="entry name" value="ATP11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126:SF0">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126">
        <property type="entry name" value="CHAPERONE ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06644">
        <property type="entry name" value="ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1093201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1127957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="40743" checksum="D74106A305158860" modified="2019-11-13" version="1">MNHLSNSIFLGLVNCPSLARPSIRSRGRQIRYLGTSDTSDRTQKLIQQKYAEKINEKLKEKGLRDLEELKKINQDKIQAERIQQRKLYDELIKKARQKLNPDTDRTTETRTSPIAKSKKYSSPIKPLDSIIDLNKIATQSPSEIRKLWTAYHLSKSDPPKLGAVIPCATYQEMLQAARKYPSFVVPLAKSSAVDSFEQAKPADVPYEMQYLQWDFVKQPAPEPRLPDFLSKTPHWTQTTPSTIPATIVMYTPLGEYKLRQTFAQPTLILTHYTDLAESHGIVLMRGDITPHSNGSPKITAIEAQMLVLRLQQFYHATPSANQDQSVHLKRRLLLESFHEQPDRFQLSELLQLVNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVI4</accession>
    <name>A0A5B0NVI4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BAG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_013850</name>
        <name evidence="5" type="ORF">PGTUg99_009299</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000381">
        <property type="protein sequence ID" value="KAA1091849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVI4"/>
    <dbReference type="OrthoDB" id="417450at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF16">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="3">
        <location>
            <begin position="206"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1089316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1091849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="257" mass="28599" checksum="0B724BE11C4967C7" modified="2019-11-13" version="1">MEVIWDSERFSVDLPVSSFTLGSFKRYLASRTGVPAEHMKLFCNAGLMKDDSAPLSAFQPEVDSPLDPGAQSSSPLTRSRLWSGIFSGTKKRQISPMRIRMVGAKETRSIVSDRPDLKPTTAPSNQNLKPQAGSNPEIMNESRIISLINELTQKALSDNLPRVERYEGMVPTERGPDEVPVDRPLNPQNTQPVQAQWDPTEFAGVSEVLLQALLKLDGFEIESEWTEARMARKEGVRAVQKLLDRLDRVKGATGPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVU2</accession>
    <name>A0A5B0NVU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024338</name>
        <name evidence="3" type="ORF">PGTUg99_005373</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000186">
        <property type="protein sequence ID" value="KAA1125056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVU2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="212" mass="23628" checksum="76BF7100B2658669" modified="2019-11-13" version="1">MSSPNTPPTPQSISSPSTAEEAIPLPEIRELHQETILETNYMLVEPPSDFHYNNTRATSPVLVERATVYPTPENEEPDIRCRVEDYSHEQVRERDLAVIMTPAEPEPCGRFGFNRASYGYYSPSAVSFFDLPGQGTADSPYYVNTPPPGAEEDADRGPFYARLSENKRCSPRVVMSRQNSLNTSEYWTCSHTAPQLVPGVVVEPLANGYIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2N7</accession>
    <name>A0A5B0P2N7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2N7"/>
    <dbReference type="OrthoDB" id="10363178at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="108" mass="11706" checksum="3FE710068DEFD4FF" modified="2019-11-13" version="1">MDPDQESSLSQVSRSPSKIPWCNPPSQYFPKPYHPKSSSLNIQGKSANLTVNIKNGPSINLDNSGGAIHAHVLLHRPTHNVGYDLKAKRADEEPSLEAKGEGGGNQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5B9</accession>
    <name>A0A5B0P5B9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028079</name>
        <name evidence="2" type="ORF">PGTUg99_030166</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1108098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5B9"/>
    <dbReference type="OrthoDB" id="2511712at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="155" mass="17674" checksum="958A9A03599CA708" modified="2019-11-13" version="1">MRGRTSDASLLSVTFSNTLRVGFIDGFSQQTLKKHKPSGPTVSKLVESLIERLYGWNEPESNHPPRLPLDHTRLGWGETRNTHHCPTHTDQKISWCMDSIVWRQKAMQTARWASLRLGPTEIVLASLGTTIKRPGRHLVYRLWRVGGLAIRTRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P6R7</accession>
    <name>A0A5B0P6R7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FPR1_1</name>
        <name evidence="9" type="ORF">PGT21_028160</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FKBP-type PPIase family. FKBP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 6"/>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P6R7"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050689">
        <property type="entry name" value="FKBP-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516:SF443">
        <property type="entry name" value="FK506-BINDING PROTEIN 59-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="27"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1096773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="10666" checksum="1D30C53B91854B9C" modified="2019-11-13" version="1">MGAPSSNSQVQVETIKPGDGKNFPVAGDKVKIHYVGTLESKDGKKFDSSRDRGRPFECQIGVGQVIKAWDQGVIQLSIGQEAYFKCPPEIVCFIFSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCA6</accession>
    <name>A0A5B0PCA6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031612</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCA6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="15713" checksum="7A2E238ED5A72525" modified="2019-11-13" version="1">MGLINLTTSSPNLFKQRMNSHSKVTDQTPIFIDSSLAISPLAEEMEISMLLTPPGYSIYDGDCFTQPEFDALFAFVMNQITIYCRDNYPKNFLEHEARVKLYMDISRGFKFANVHKLIPKYEDGMPHGSSSGGNNTVVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PDZ9</accession>
    <name>A0A5B0PDZ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000057">
        <property type="protein sequence ID" value="KAA1098900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PDZ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="13083" checksum="6B38BC3FFEE6555F" modified="2019-11-13" version="1" fragment="single">PLCHQELNTSPTTPSPRVPDQPYFKVRGLAVPQKCRAQQRGLVPQGLHVYFDQQAAFSSKPHPETTPPFTHLGQAKSGHQRPVSNVQGSGISDRHELCQLIIRISYLNAWDLGEFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHN9</accession>
    <name>A0A5B0PHN9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">FRS1_1</name>
        <name evidence="18" type="synonym">FRS1_2</name>
        <name evidence="17" type="ORF">PGT21_029812</name>
        <name evidence="18" type="ORF">PGTUg99_024971</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="18 20">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHN9"/>
    <dbReference type="OrthoDB" id="1698572at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000004">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000023">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="16">
        <location>
            <begin position="297"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1100114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1120713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="635" mass="72056" checksum="026060E94B0AE335" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHX2</accession>
    <name>A0A5B0PHX2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028822</name>
        <name evidence="4" type="ORF">PGTUg99_016726</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000144">
        <property type="protein sequence ID" value="KAA1128152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHX2"/>
    <dbReference type="OrthoDB" id="2515503at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474282" evidence="2">
        <location>
            <begin position="19"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1128152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="618" mass="72664" checksum="5A20A1D532301195" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKV0</accession>
    <name>A0A5B0PKV0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_050203</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKV0"/>
    <dbReference type="OrthoDB" id="10289261at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022826934" evidence="2">
        <location>
            <begin position="20"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="149" mass="17171" checksum="8F0D673E5DFC4063" modified="2019-11-13" version="1">MSVQKCLLLVLLVASVALGRTVVNLDQRTGRLEKRIRVPLFKDPLNRVINIDDRHYKDNFPGGIHKHPKNEKNGFAKKKYFTKEQEEAIFEQHQKVNLAKEKLDEAIAKQQQSRNSGSEERAKAQERVDSAQRKLIEEITKLEQLRGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLP6</accession>
    <name>A0A5B0PLP6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_033784</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLP6"/>
    <dbReference type="OrthoDB" id="2508778at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="5">
        <location>
            <begin position="515"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="286"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="296"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1101936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="681" mass="76230" checksum="A6FA20C6745E4D34" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PM22</accession>
    <name>A0A5B0PM22_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PM22"/>
    <dbReference type="OrthoDB" id="10272316at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="126" mass="13023" checksum="88C47CCA6B901EFB" modified="2019-11-13" version="1">MVIAVQSDTGGPAPNPGFTCKNIQGKTEASCFVQQDDSGTSKYYVALAPNASTDDKYTCESVKIQKTAAQGTTCCTPGTSPKPADPEKDPQPTPEAPPAGMTEEQYKSACGKADDPASTSTTEADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PN95</accession>
    <name>A0A5B0PN95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PN95"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="11628" checksum="5770D1924273B2DE" modified="2019-11-13" version="1">MSSVRFFKRIPATTDRFFQQISEQDGDYFEEIDFSQLQRPVNRLFTSGRFGERGEKINLREQSDSSETTANAELKQSPWCKADEPSCPSFGDPAKCEPPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNF0</accession>
    <name>A0A5B0PNF0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATP-dependent DNA helicase sgs1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNF0"/>
    <dbReference type="OrthoDB" id="2506785at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="30109" checksum="9BD7E7EF11D5BDA1" modified="2019-11-13" version="1">MAKCECSNCYPVEAATLIEALSVANNENFDDILNNNFTLTSTYNIKHKYPQKAPGIKKRKFTEDDVPEMDQFATMLMNDFNHHYETNVRPGGSTQGSDIFDMDDCLAILAQLEYITDPVYLKWIIGGECFAGQSSWLFNWITNYKLEPISPQISSKKGPLAKKAKQITSCAQSTSAPMDRSVLQVVTPRQPNKKELAAIESRRKALERQEAKRREEEKTQRRKEQIQQIMASSRAAQLIGPETHHTDNPHSQSSNGNQAYFISKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PS48</accession>
    <name>A0A5B0PS48_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019527</name>
        <name evidence="3" type="ORF">PGTUg99_009581</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000279">
        <property type="protein sequence ID" value="KAA1112292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PS48"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="86" mass="9215" checksum="B8E1F4B0DE5110EE" modified="2019-11-13" version="1">MQGMDNEADSRPTDLKLASGLKADGKVKGVHCAGQSAPAGHASATTTQQTNSAQPKSPARIYRLTLHDRFTSPSVYNIFIHHQPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT22</accession>
    <name>A0A5B0PT22_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450-dit2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_001678</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT22"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1103822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="542" mass="61726" checksum="21AFC24849CE37E2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTE5</accession>
    <name>A0A5B0PTE5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTE5"/>
    <dbReference type="OrthoDB" id="10292303at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="188" mass="20579" checksum="BBBE1BA02BA59928" modified="2019-11-13" version="1">MRQRLTLLILYYVKAVNSMGQPEPLGLSHAVNEAEEVPVATMAVDRSAMSSHNPLSPKISTPGTSQMLDSQTTQEDKTGQIGTITWPVNESPPHEVPEERENKPPGSLESSRATDHAEEGNPVPAATTGVHRGTMNSDNPLSPKSSVPGVSDQFSQNAQQENNRRVDPIQWPVTEKPSEVPEERKFYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXA8</accession>
    <name>A0A5B0PXA8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Polar growth protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BOI2_3</name>
        <name evidence="4" type="ORF">PGT21_011574</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXA8"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="3">
        <location>
            <begin position="251"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1105552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="478" mass="52226" checksum="90D4F881B2D76803" modified="2019-11-13" version="1">MRAPQISGIHTVEEEAGDIPESSCIKGCDSFCDGPTQVSPSIRCPSGGVQHEKRGLGGMLGFSPPSQSSNPQATGPPRDPSAPVNGTCKALPLTRETWKTLNLDEYLRTYPGGDHLTVSEHPTLTAVLETSVTLARYGGLRMNPFAKPRASLSNNCLLPYSSAIPFPRRTATISIILSALFPAMDPRMVQMIKLNFAVNAAFMMVSNTLFLDILVLFNSPQLGWNLFFNVLNNIINAASYGAAAFLPVPKEEKHEAFTEWSHLVDGLAAYEQKMIDGISQESHKILNAPISSKDGMADILKNGTYIAPLEQVFLPAIETALKNVTTALFLTRILRSMDAFVTIGSDVCNNKGPNGALEGDDKLSYCDSSGTMYNIVRVSDEKGKKHKDKEERTFPNAFVIENRLGFSTQFLTNASLSCQQKYGGFEHFPWVNTTIPRDHSADCVVNLPVCDLRNRGDLHHTIKKHGIVAACRQIGLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXH5</accession>
    <name>A0A5B0PXH5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXH5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="56" mass="6112" checksum="F59E0C9BF63826F9" modified="2019-11-13" version="1">MFRSGQEFFFDGFLAGWDLKDHVAIIQVLTVSPVGIGNQNSTLKGPSSPVNKGQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4E7</accession>
    <name>A0A5B0Q4E7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4E7"/>
    <dbReference type="OrthoDB" id="162969at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="12499" checksum="B54F2C6D417DA99C" modified="2019-11-13" version="1">MSVSELLNPAGESEYSVWTTEEIFKSVTDQNTEEDQDNNESSKPPPRPKPTAKEVFKAISLINDYIENDTTEVADKLNQSMEAYSKQLVDHLIATAQQTSITSFFHPMNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5K3</accession>
    <name>A0A5B0Q5K3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="9">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GPD1_2</name>
        <name evidence="12" type="synonym">GPD1_1</name>
        <name evidence="13" type="ORF">PGT21_009616</name>
        <name evidence="14" type="ORF">PGT21_010286</name>
        <name evidence="12" type="ORF">PGTUg99_032945</name>
        <name evidence="15" type="ORF">PGTUg99_034259</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="12 17">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 9">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="9"/>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000002">
        <property type="protein sequence ID" value="KAA1138674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5K3"/>
    <dbReference type="OrthoDB" id="10263760at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000004">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000365">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017751">
        <property type="entry name" value="G3P_DH_NAD-dep_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03376">
        <property type="entry name" value="glycerol3P_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF8">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="11">
        <location>
            <begin position="198"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="274"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1083424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1094097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1108372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1138674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="347" mass="37532" checksum="935E8C42B05115AA" modified="2019-11-13" version="1">MSSAKQQVAIIGSGNWGSAIAKLAAENAAKHSDHFEKEVPMWVYEEQVNGKNLTSIINEDHENVKYLPGVQLPTNLKAVPSLTEACKSATMLVFCLPHQFIPNICRQLREAKVIGTGAFAVSLTKGVDVSGDKIKIIADVIEEELGISCAALSGANIANEVALNKFSETTIGYRVKKEGELWQRLFHTPNFHVQIIEDRAGVSLCGALKNVVAVAAGFCDGLGWGNNAKAAIMRIGLLEMKSFSMEFFSGVKAETFVQESAGIADLITTCFGGRNRLCAEAYVKTGKPFSQLETELLGGQKLQGVATAQELNTFLKAKGRVEGYPLFSVVYKIAFENLDPAKMTSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5U4</accession>
    <name>A0A5B0Q5U4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5U4"/>
    <dbReference type="OMA" id="KSATHDE"/>
    <dbReference type="OrthoDB" id="2495569at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="343"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="808"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="818"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="831" mass="93721" checksum="C7459ED039F14D1F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAD6</accession>
    <name>A0A5B0QAD6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_013703</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAD6"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
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        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
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        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="107"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1110188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="447" mass="50755" checksum="9DF969309395DB67" modified="2019-11-13" version="1">MASAYIAHLGDLSTLFSFVGWWFLPGYVSSTVLGIFYRIFPARRPKAPERGSTNPDEKPIYNPIHELQKSEYDRRARRHHSIAHGLVIGLYLGYTLIVDYQKQTGSNYYSVLGIQSNSLAFNSTTQSVPENLNTVRTQIIQGDLLDEAGLKSHWRRVARSFHPDKLNPPAGLRTEIEIAEWKGNVEAKFIRMREAYETLNDNTKRWAYDRFGPMILQWKNCITLREYLIEGIKNTCPFYGFSFSSLLVIGTLRKTDAGTFWRWTTMLLVIGLEAMILTSTSLTRVSLVLSRIFPNRAPFEHIELLRQFFIASSCVATQISGIIYSVPEEMNKNRSLEKAIEPMLGLVDQVQEYSSLANVELIKLMFNDLHPIIRSPSGKDKSGNDSLNTIPEAEANQLSQDQSVEVQKVISDVKNKMLDTFCDLKLQSDPNGQAAWIQAIRSKDSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAJ9</accession>
    <name>A0A5B0QAJ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005588</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAJ9"/>
    <dbReference type="OrthoDB" id="10366534at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023138424" evidence="2">
        <location>
            <begin position="25"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="419"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="594" mass="60231" checksum="2B541627A5079B56" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBJ4</accession>
    <name>A0A5B0QBJ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBJ4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="211" mass="22725" checksum="29FBDDF007336001" modified="2019-11-13" version="1">MLREQIKVENATTLAPSKRQQALDEHRQSGQSAVHSPSTATSSTLGTSSPIPPSRSSELASSSARASFKLNATQFHALSGDFNELSSFITQNHLDQHHPTRSRAINSLQASLLDHLELSNPVFPFPQTTAWAAAASLSLASFARAIAIFCSISGCMQLHTQGALVSDPLRIGSIAHPPLAHQPPCSDSTLYDHLKQPAPTSYKMHTTDHLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFS7</accession>
    <name>A0A5B0QFS7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Phosphoglucomutase-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PGM3_1</name>
        <name evidence="14" type="ORF">PGT21_017325</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFS7"/>
    <dbReference type="OrthoDB" id="8300170at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05799">
        <property type="entry name" value="PGM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000035">
        <property type="entry name" value="Pgm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="50"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="219"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="343"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1111959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="615" mass="68123" checksum="F3E5A028025B5610" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QGM8</accession>
    <name>A0A5B0QGM8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein phosphatase 1 regulatory subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_020035</name>
        <name evidence="5" type="ORF">PGTUg99_013168</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000279">
        <property type="protein sequence ID" value="KAA1112302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QGM8"/>
    <dbReference type="OrthoDB" id="266138at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1103503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1112302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="45259" checksum="70E97F6766060513" modified="2019-11-13" version="1">MSETKPTDSEPIQSPTIDSAVARLDEASYQDGIQERQAILEAQAIEGAKDESVWQAENEELLTEFKEGTEELELSHSRIRTLRGFERHLERLAPSLKRINLRQNLINQLKFNPRIDSKATPLEIKDNQPPENSQDETPDQVLLEVIAPFGHLEGLEELDLYDNQIAKIEGLENLHNLKTLDFSFNLIRRIENLDSLRSLTTLYLIQNKISQIEALDNLANTLTSLELGSNRIRQITNLACLTNLTELWLGRNKISKLEGLDALVNLRSLSIQSNRIVKLEGLENLVGLEELYISHNGLTSIGEGLSTNKKLRVLDVGANEINDMSGIEGLSQLEELWANNNKLTVAGWSAIEGSLSQSNMANLQTVYLEGNPLQLEMGANYRRKIMLACPQLIQIDATFVKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIY2</accession>
    <name>A0A5B0QIY2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIY2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="10860" checksum="EF195D7FC916F4CD" modified="2019-11-13" version="1">MGISPGDGNLVVYSKTLESDIGTAMKLVSRPNVPSFLNAEHKLRPELLHFLSLVFQHRLSFIRASGYKQHAASTWPASSVISALGAEPGAALLLIHAEPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJB4</accession>
    <name>A0A5B0QJB4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022442</name>
        <name evidence="2" type="ORF">PGTUg99_020902</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000276">
        <property type="protein sequence ID" value="KAA1113692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJB4"/>
    <dbReference type="OrthoDB" id="2495703at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="520" mass="58727" checksum="62D8ADDFA48C627B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJD0</accession>
    <name>A0A5B0QJD0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heat shock factor-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023729</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HSBP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJD0"/>
    <dbReference type="OrthoDB" id="2506224at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009643">
        <property type="entry name" value="HS1-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06825">
        <property type="entry name" value="HSBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="10"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="21626" checksum="232591B9109EF1F5" modified="2019-11-13" version="1">MYGCGCNHTGYPAHPGVSMSQSPGVDFRNKPKGIQGPKNADKKNHNQPNPSQKMINGLGRINENSQSTSHKNFSSTQTTSALKNSSSATASGSSTAVGNVAGTTTTTATSTSNNQDQAGPDFNPNDICSPHELIGWVDEVLEKLEAKFSRIETDVIERLDVLGTRIDSLELSMSGLLDGASNTTTTTTTTTQQPHESPKKSTDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJQ0</accession>
    <name>A0A5B0QJQ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJQ0"/>
    <dbReference type="OrthoDB" id="10336190at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="216" mass="24195" checksum="C5E07883A9394A1B" modified="2019-11-13" version="1">MSAPGPFQINTGFAGGQAFSQGNTDSTSQAQHSANPVPASNTANDTPDLQYTDRTNQPEDTDANASVSHNPNTLQSPSLPEDEDGLRAMMGDPPNKNRPPITSDQHKLTKEQMEQFATLELDALRVAANTHAIHRRMTEEMREEIDEMYYEFQCNVTKLAIQNRVASHLYFKHLGQNRKVRAGTSWNNFQKYDPAVQRLFDECKFPAARSIFAYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QLP7</accession>
    <name>A0A5B0QLP7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_037311</name>
        <name evidence="1" type="ORF">PGTUg99_009893</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000273">
        <property type="protein sequence ID" value="KAA1114033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QLP7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="94" mass="10211" checksum="F417734827B18CAD" modified="2019-11-13" version="1">MSTCHICLFKNKNIQAVKTYLQPAAGYQGLKRNYVCFEKSVIGDFEKIPKILQGDGDIPPSSWKGSGSPTSPMYIAAPELPCLGRAQDAVDTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMD3</accession>
    <name>A0A5B0QMD3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMD3"/>
    <dbReference type="OrthoDB" id="2496225at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="576" mass="66303" checksum="C5AF7E23F87AEA13" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN33</accession>
    <name>A0A5B0QN33_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein required for ethanol metabolism</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_014085</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN33"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF50">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YOR292C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="306"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1114572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="38533" checksum="A25ABD6F4C9B33A9" modified="2019-11-13" version="1">MPSLSRLTRSYERAFKHHPSLTLAITNGCLKCLGDFLAQYLPAFSSGQPFVLDIHRSLRFFLFGFLHGPCVGKWHEFLERRIPLTGGRATSNNGVTEPGATPEMEQEDLRLIETEKSLTSHRPISIRSRSTTGPEHHPGALRGLSNSDAPASSFPHNNNQHLTGLSKLGNPTRAAATGFRVFGLLKRILLDQLLMAPIYTFLFISLTGWFEGLSIPEIQQRLHQLYWYLLTANWKIWPLIQIFNFSFMPLQYRVPWQGSCGVLWTVFLSLSTHSSTHYSSSTTTVDPTGPTLVASTLSKLFKSGNSSASDPHLISSNHLANSSSAPSSSSSSNDPSSPIKRKNNRVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN63</accession>
    <name>A0A5B0QN63_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN63"/>
    <dbReference type="OrthoDB" id="10534660at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22744">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="41208" checksum="396A4A5B9CA1FA9D" modified="2019-11-13" version="1">MSDKSSNRPIPAGHRGHIPSLPPNLAGPPPHHPSRQRDPSWAQAHDGRQDTPVFQGHPGAMSGNHSWQPMLAHSSHHPMNYSGPPQYMSYSAPMTYVAPTSYQHSGPVYTGQPHYPYSAAPQSHFPPMGQPGPPYYHHPSMPIYAPLPSQQMFFPGHSEPTHWPIQTNGPHPPVNYGSRPCPAATSGQPSNPSAAPQLVFAYKPESLYAKTNGWYHVRQDLIHELDFNKIHWTRRFGSDDEYKRVRESLVVEANSISVPYDKWMERLDMGPVLANAYNRPIVFISDDVKIGCITNLPSLKDPDPKPLGPILIAFTRGNHWELVIPKRGLIPIPPLSFSFRTRLENASMAQWLTAIQPNVDLYHQLISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNS7</accession>
    <name>A0A5B0QNS7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
            <shortName evidence="3">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CKB2_2</name>
        <name evidence="5" type="synonym">CKB2_1</name>
        <name evidence="7" type="ORF">PGT21_016139</name>
        <name evidence="5" type="ORF">PGTUg99_014608</name>
        <name evidence="6" type="ORF">PGTUg99_033303</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="5 9">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000345">
        <property type="protein sequence ID" value="KAA1098852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000338">
        <property type="protein sequence ID" value="KAA1102406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNS7"/>
    <dbReference type="OrthoDB" id="3971593at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000003">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000002">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF39">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1098852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1102406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1114625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="270" mass="30553" checksum="0355A5B2A2909DB2" modified="2019-11-13" version="1">MDELSTGSESDYANSWVTWFLSTKGNEYFCEVDEEYILDRFNLTGLNSEVHRYGMALDLITDAFGVDELDEATRESVEGAARHLYGLIHARYIITSRGLAKMLDKYKKADFGRCPRVLCNTQPLLPVGLSDTPYSKAVKLYCPRCEDIYSPKSSRHGTIDGAYFGTTFPHMLFMVYPNMVPTKLWRLGTISNSNQGGPPPSEVGQMVGTSSNNNTPQTGSLDSALKAQRYRPRIFGFKVHQIAKLQRWQESLRDEQIDRLKKLEEQGNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNV4</accession>
    <name>A0A5B0QNV4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNV4"/>
    <dbReference type="OrthoDB" id="10519097at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="327" mass="37136" checksum="65D95F0AEAB25953" modified="2019-11-13" version="1">MSDSPSYPTLTYDIISTTIKRESEVTYSYYWKVKEIRTYQVTTQKTYLQSNNELLGQNQHKRVISIIIKKTRQNLDTATNNPTTSSRTTEVHSNNTEVGIIREPRTSPTPDESPRRGEDNRNPLPDPDQRGTTPTTTRRDSVNSRESTGKKDIYPNYLNQSNNLTEMFDYNNRSRDNNGEPTSEYYQCRGFPTAPNRREVRKVLLQDDGKQKLVRTSHEPSNRGSYQSPPKLLPRGFLRNDSVARTGQYIDQDSRMGSQLDPSIPLELVSGYPLRISVIRWRIPAVCSGYPPADAGSGADVPFPLIFQRIPEAGRADGRLEGFPSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPC6</accession>
    <name>A0A5B0QPC6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPC6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="425" mass="49581" checksum="DB1D8076A37FDCF0" modified="2019-11-13" version="1">MGGKGDVFAHDSSQMLQTKGLNSGQDRGLHVQTQNGQRTKAQTLPEESPHLSDWVPSTKSQLQGGYITLPEYRAISIRVLHSEPKRVESFLEQFRATKVIEDAKNDGQIFKSSDIIRDHINWSMKNDKQNSYVRHWETGKFSIKQLTEKFQGNFNTLHLSDYVELLAHNPEYHNEMEKFRSFVLEGGGFNYQDFTGSSWDYNSVKEIFQQWKTLKSPNWKDSPETKGKLISLDEFKRRLKNSFEFEEEIMNMIDRDPSLNKKYLNFYSPSARKDLLWLPNLISYTFYWLNKNDKMPASILEKIQSGKTLKEISKEVELRFNQMELHMGKDTRSQGPTGMKIITHSGLVQETDTSLLRWIPSTKSELDNGYMKLREYSAIIRRLLEIPRGKMTEFVDHFKEIRGIQVLKMILSMLLLAQFWEGNPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ34</accession>
    <name>A0A5B0QQ34_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ34"/>
    <dbReference type="OrthoDB" id="10272894at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="259" mass="28383" checksum="3AEB84FD13F5A7EC" modified="2019-11-13" version="1">MNVVMPQRNGFPEPAIGSQQTQNEIAHVAGQSRASPEPTTALQQLEIVHVENGNHPARGEANPAHISDTAVLDAASLALIDGIRASQDRDVTSNENNNQTNRASPRPPLRAMMNLDYPRIRSMLLGSNPNPPKKANGRPASSTDMQSYNGAVEVSPNPPNTEMLGLQYHAIRAALLGIQQAPPRNHAPNQPGEQPREITEQEIVEFLEECAGEFVRYSRDSQASCEAELEARVKLVAQMLCNSKKAVLIQERLFLRFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ58</accession>
    <name>A0A5B0QQ58_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ58"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="145" mass="16879" checksum="7A41553280E058A4" modified="2019-11-13" version="1">MLDSFFSSPGHTQTDCHWVLLSSLDCVCSFIHRSLFTKRQSALALPLRVRSANWLSRLPPQLLHHQIQYINIYNCHPPSSFLFNPDRPARTLNQRHSTQASNQQKTNPQIAELDQESHPRFFRPTPNHQLELDRKDLGRKLISKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QRC0</accession>
    <name>A0A5B0QRC0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_017529</name>
        <name evidence="4" type="ORF">PGTUg99_031141</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QRC0"/>
    <dbReference type="OrthoDB" id="2500743at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138044" evidence="2">
        <location>
            <begin position="25"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1115768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="347" mass="36759" checksum="1D0F148AAB37138B" modified="2019-11-13" version="1">MILPSVITLAICLFLGKDPSFTYANSLAVRQGPVHLQALKAAPLGRRNLPGLDLFSHHGGGRKQHGHGSASPSYEPHGEYGNDSSSYGQDSYDPSKGAYGSGNKIDPTGSDNGQGAFDLGTGTHHALQEGTLDSGDGGYTASDPGAGISPEPNASANYGPKKDNYHSEPEKKAPSTEDNDYATPSKTYNSGKGSTSSYQDPYSPSSKNSGKKDDSGLKDLGEYKQPPPEDNSKSSSGTDYYKKKNPKYHDKYASPHGKQKNGNDGKDENSDQDGSFIFESGTTEEQRKAAQKEANKDGAFKKGGKNDEGKSSYGGPVDPSNEKTYSGDQSYKQPNVTGGQKDSSPYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSC1</accession>
    <name>A0A5B0QSC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003187</name>
        <name evidence="4" type="ORF">PGTUg99_002630</name>
        <name evidence="3" type="ORF">PGTUg99_022772</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000109">
        <property type="protein sequence ID" value="KAA1130464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000010">
        <property type="protein sequence ID" value="KAA1137211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSC1"/>
    <dbReference type="OrthoDB" id="2351920at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="208" mass="23117" checksum="1F20B430065EF046" modified="2019-11-13" version="1">MSDDRFTYIHLRSETSLWSYTDDQVDWTLEDLEDRVDQSAWDMIPPYQNENDEGILPSHSHPSYPFGNPYDPTQTPVQAPQLARVMNQPLAVGSALLLPPSHGEQGQSYWAELATDRLLRASGPTEPLDDLHPDDLPPDELDHPHHQPADPSQLLDHTPGGLNPQLRTPAPDAYRPPRPPAVPATDQTESFGSEDDDEQSGETGAMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSD0</accession>
    <name>A0A5B0QSD0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001933</name>
        <name evidence="2" type="ORF">PGTUg99_009744</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000046">
        <property type="protein sequence ID" value="KAA1134719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSD0"/>
    <dbReference type="OrthoDB" id="10305548at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1134719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="411" mass="47788" checksum="F1C000FDD14BF40A" modified="2019-11-13" version="1">MPMPHSSMIICFLMLTRFGAICMYYPLSRLIHNPLGDFPSYLSPEQQNLLRGRAWGKINDEEWAEAQNMQIRKLGFFPEYLDPVNKEWLYGKIYPPSFRHVSPYEVDRNLGSLSPRMYINSDRMSREFRMGIDPENSSQGFNESYQHRYLHDSNSFFFGDASHKSYSKSSLKEVVRNKNDPSELTLDQLDKKGWRFPKGLAHQSQFDKMPVLMDLKKALQAMREFHRRGAHTEGSTVSSSIKGNKLKGNWDHLDHDMGKLEIWENAHKDKRTRFESGTTELIPPYKITLEETWDYIYKALQVTHFLPQDEKGHLFFANLQILLADALEIGTKQEQKTSFAQRFAANLRPLLAQPQFPRLLSEASGREILRPMDRLSKWLKQNAELEFSNCEEHTTHTDKVPQAIPSVLVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QUQ1</accession>
    <name>A0A5B0QUQ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QUQ1"/>
    <dbReference type="OrthoDB" id="2499859at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022823885" evidence="2">
        <location>
            <begin position="27"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="894"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="959"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1031"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1122"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="594"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="982"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="998"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1048"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1067"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1092"/>
            <end position="1102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1178" mass="128046" checksum="552B34431A9D17B7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVW4</accession>
    <name>A0A5B0QVW4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cytochrome b subunit of succinate dehydrogenase, Sdh3p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SDH3_1</name>
        <name evidence="9" type="synonym">SDH3_2</name>
        <name evidence="8" type="synonym">SDH3_3</name>
        <name evidence="11" type="ORF">PGT21_007830</name>
        <name evidence="9" type="ORF">PGT21_008072</name>
        <name evidence="8" type="ORF">PGT21_023840</name>
        <name evidence="10" type="ORF">PGTUg99_013182</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVW4"/>
    <dbReference type="OMA" id="PHDATHY"/>
    <dbReference type="OrthoDB" id="588261at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03499">
        <property type="entry name" value="SQR_TypeC_SdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.10:FF:000011">
        <property type="entry name" value="Succinate dehydrogenase cytochrome b560 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018495">
        <property type="entry name" value="Succ_DH_cyt_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978:SF5">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01001">
        <property type="entry name" value="SDH_CYT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1085913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1099459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1113752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1117452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="189" mass="20544" checksum="CD99F71B000A1069" modified="2019-11-13" version="1">MSSTILQQAFLRTSLKPVSRLGVQTSCFKSPVLGSLSSLHQQRFSSSKTLKCSEEDAIKLLNEQRKLRPSSPHFTIYQPQLTWYSSIANRVTGCALSAGFYAYALGYMALPGSIPLDSDTVVQVVASSPEWAKVAGKAIVALPFTYHTFNGVRHLAWDMGYLLDLKTSYTAGYTVLGLTAVSTVGLALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R017</accession>
    <name>A0A5B0R017_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HD/PDEase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R017"/>
    <dbReference type="OrthoDB" id="9991235at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000026">
        <property type="entry name" value="Metal-dependent phosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="2">
        <location>
            <begin position="99"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="405" mass="44444" checksum="1A380DD9F0D47E32" modified="2019-11-13" version="1">MASVSTCQNLGFDFNLREIGTINHTPLENDDNLTSTTGDEGSAKNANPSACIEIDDLIYGKVLIESPCAVEIINLPEFQRLKDVLQHGITALIGLLPSPPVNRFDHSVGAMILVKHVGGSEEAQLAALLHDVSHTALSHVTDSVFGYVVHEVEKGDFLDKTQIPNVLQKHGFDPERVFEEKLFPLLELPSPEICADRLDYALRDSVSFGYLALKDAQEIYRSTISIDGKMVLSSTEAAKQLAEAYLMSDQKVWADGAQSYLYKLAADAIRLSLKEGTITKEALWTCGDQAFWDLLVENASAQVKKIVEQVNCRCTLIETNEQVAPVGGSEEHSGQPTSASEEEAPPAERCTLKIRVRTIDPPVKTAEGIQPLSALDPSFGHRRLDYIRSRQTPVTFIVKYSPLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1H1</accession>
    <name>A0A5B0R1H1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023239</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1H1"/>
    <dbReference type="OrthoDB" id="2509318at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="343" mass="39344" checksum="A1D3F6AB51FC7CDB" modified="2019-11-13" version="1">MTMTDRSVSHHDDDTIEEVCDKLQSQLKLDPQHLKIALITSKVNPSTWSCSSPISHFQCSICFHQKFVPESGTAAVIFANAAYHQLDGPNTGIATQRTANFVFDQSFRVTIKQNQQDFVRKTARIILLLPTLEAYSNNPHKNGALPKTLFYQTLPDAWKEEHLQPSSNQDIPQSPSAYREAVRDLLKYQRSNLRVLLLRNILETKRIFITDHVPNRKAMLTSIYEDLPPKGGKMTTAKITHQVTTNYAMRARMCYARLVMVYFYVHKSKKDLQWSDIDERLAVLRGSSCEFQQHHSILVLNKDFLLFSQKRNYKSMKPEEFSVPTLEEVQDLVDTGTIPATLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2I0</accession>
    <name>A0A5B0R2I0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015728</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2I0"/>
    <dbReference type="OrthoDB" id="2564904at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022786421" evidence="2">
        <location>
            <begin position="22"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="340"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="409" mass="42608" checksum="0F1009CF902C8330" modified="2019-11-13" version="1">MISVHKLLLVTLFCAAGQSLAQSQPTQPSNSNPAPAAAPAGGEGQSSTSGDVPLYQKRFTWPNLPYQADTGNGPRGQQQGYNRCNATTQNQDSMCQTTFINSIQDMSFFTLCLSKLFPEPRQSPESIVGNIEGEMVAYCTTKKFGTRLIPAGAITGLQVTRTPGYIQAVGFIQQTALNIQANDTGGEEDPHGADQRGNPLGALMYSSAFNTPGGPAYTQVIEWSYFIGSGVFCYKACDPAGPNAAQLCQHIYDRIGCTYNAPAHYETINGTFTSCQGENQLPAGTYVENGVTKTYTQPPESAGPITSIPYKAAIPAVSNCQTFTDTKALWPDLPQMASVSNSPNSSTPTQNKGPNGSSGGRSSSSSSSSSGSSSGSAGSNAPSSAAAAAVVLSSATFFGCALLATAMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3K5</accession>
    <name>A0A5B0R3K5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Anthranilate synthase / indole-3-glycerol phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TRP3</name>
        <name evidence="2" type="ORF">PGT21_037171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3K5"/>
    <dbReference type="OrthoDB" id="1865897at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="1">
        <location>
            <begin position="35"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="169" mass="18348" checksum="8699D44C3567E990" modified="2019-11-13" version="1">MLTADRLPAGQICGRGWSVCPANTSPGCGRAAVVVYIFYINCGQAVQLIGASPECLYKLSPGGVVKNHAIAGTIRRGKSLAEDEVLAQELLGSIKDRAEHVMLVDLARNDVNRVCWPETVKVDSLMAVEKFSQVMHLTSQVSGVLREGLTRINVEKVPDLTRREESKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R431</accession>
    <name>A0A5B0R431_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_037216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R431"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1120159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="126" mass="13892" checksum="AF37D8FD0D21C1F5" modified="2019-11-13" version="1">MGKPTEPLWKIGRSLYGKSDGASMEKPILYGKTDPLWGNRLSLYGKSDGASMEKRTKPLCKNRSSMGKPILYGKTDPLWGNLSLYGKSDGASMGKLTEPLWKIGRSLFGKTDQASMEKPILYGETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R6K0</accession>
    <name>A0A5B0R6K0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031991</name>
        <name evidence="3" type="ORF">PGTUg99_010193</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R6K0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138065" evidence="1">
        <location>
            <begin position="24"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="241" mass="26043" checksum="83D6C383E3A1F93B" modified="2019-11-13" version="1">MASFILFFVAMSLLANQISLSDAAKCNDGFNLDLNECKVARDSIAYVPGKDLIKSIERSIQVSSGNCMIQIDVVPTPPPVISKKEIDETINDILSCATHAGINDPVKNIQVQISSIPSSYGATYGPNQKMDTAVCNTDADGGKNEQQDCLKAFNKITTNKLGQLLDENGQATNKIIRKQNSCVVQISSTNGLPVTEKLDNLFKSLNIILNTCKKQSGYLARLRGTDGLNGRLSVKTLRAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RG42</accession>
    <name>A0A5B0RG42_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014980</name>
        <name evidence="4" type="ORF">PGTUg99_032654</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RG42"/>
    <dbReference type="OrthoDB" id="10315180at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138111" description="Secreted protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1124720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="14677" checksum="F32FDB8614B3D31F" modified="2019-11-13" version="1">MRTSLMTATTLTIFLLFTAAISALILCPNCGSGTVLQSQIMHCRIEQVCRMGVSHGPCPGTKLQAWRKCTYCPWSLPLMGYYKCDLIHNIDHCYEQHPKRRKLYSPPQQETTTTAPLPPMTTTIDSASSVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RG75</accession>
    <name>A0A5B0RG75_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010556</name>
        <name evidence="2" type="ORF">PGTUg99_036028</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RG75"/>
    <dbReference type="OrthoDB" id="10427895at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1124856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="79" mass="8328" checksum="44FE23B8CC21F8D8" modified="2019-11-13" version="1">MGRPPPRCVAEQRLGGVFTLHGSHGVPGYAEVAPGSPGCVCEGISDYEKSTKTQLTRADLQETFTSSPNTFSDLSSLVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJ03</accession>
    <name>A0A5B0RJ03_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008729</name>
        <name evidence="3" type="ORF">PGTUg99_036679</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJ03"/>
    <dbReference type="OrthoDB" id="3364670at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="45838" checksum="FCB63BE3838DA81A" modified="2019-11-13" version="1">MACRSCPACFGPAPENLRDYTHLKENKLIVCLDGNFQHRHHSKASRDYERIHTPSLFISQSEVDAMTAKIRDIELRKKPQNKKDRCTEAHKAADDKRNESSWKGCDDTGLMGCCCRHDAAIYVANINKSGEQRCFPMALLNKIINNVESDRHVGILYDIGCSLDKFMTLRGLLDDKRSQLQFGTSAFHAYVHSWTCQLDYNPRLNEGWGLSDGEGLERMWSYLSPLVSPLRYATRNHRLAAISHRLKYHNNRGIKQLPIWLKKKFIAAVRRRVETKAVLLELLDKPNPFSTTGANYTKRFFKAQWQEQCKFKETHTEEEEDHRAKLLALYKQEAALEAMRSRLRSNPSAYLNDEQDVHELLDELEETAENLKKAAEELGRPKQPAPDEADGKMFLHHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RKY0</accession>
    <name>A0A5B0RKY0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021551</name>
        <name evidence="3" type="ORF">PGTUg99_017036</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000175">
        <property type="protein sequence ID" value="KAA1125768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RKY0"/>
    <dbReference type="OrthoDB" id="2501098at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138180" evidence="1">
        <location>
            <begin position="20"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="225" mass="24897" checksum="26959ABC3925550F" modified="2019-11-13" version="1">MLRLSAIVYVCSALSHVLSTPLVPAHLPSAPGPYLRNVHAAPAAEGALPRRMPEFVPDFEPEHVQEYTILKDGTIRQRHRKLLGEESTIPVPPPSVAMYNDYDTRKMTPDEEQWVKHFAKTHNQGRPYRLTGENQFTEIPKENCDHDYFCGRMRFYDGEDKLLHDTGCCHCNASCLSVREANAAEVGIGIGVFFGAIAAVVTGVLGCAGMCAICEKTHCCTCCCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S767</accession>
    <name>A0A5B0S767_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003364</name>
        <name evidence="3" type="ORF">PGTUg99_030453</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S767"/>
    <dbReference type="OrthoDB" id="10297183at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366709" evidence="1">
        <location>
            <begin position="21"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="235" mass="26627" checksum="852BD427B7E61E4C" modified="2019-11-13" version="1">MFKCHHIIILACSIPRYIVAAPTEVAAFAPHHDSFLNAPPPLQFTLEHDNQLQLRNRRFLHETIPPEVHPPIPDKMDRNGIKIRKPSQEQLILIRYLIKHVTAGRPYKFEEDGSFHDVPKSPWTVKYYCGKIEVFDEEDYLLYETSCCHRFPHSSAYCCSCDNLGEALLNTFAIICGPCTIVPSVSAIFLMAKEVGPRTIQRIRDAFSGTPAALWMRQQKATVVPAPETELGNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S801</accession>
    <name>A0A5B0S801_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004744</name>
        <name evidence="3" type="ORF">PGTUg99_034257</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S801"/>
    <dbReference type="OrthoDB" id="2505172at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="389" mass="42997" checksum="0FD917F3F71AD0BA" modified="2019-11-13" version="1">MSEIQNPPPDGVDSQAAKVLDTQKDNEKSQWDVEKNQSKNSEHQNGSERANHQNPRGSSRGQSRVGRGRGSKVSRGPRGRRQRGSADDDRSFPAHKTGDDVKEVQVKEKTSRGPSKGNGESASSHSENSEDDEDEDDEDEDEEADDDEEESQDDDEEEEHRPNDKTEEHENLDENVTVKDIQSPDTSTSKHAEASAKGLQKKQPYVRQVDLPKEKLSPADLEAKMAAMALKNAQLIEQKKAADQDAAEYREIELKSQRTFEAQRAQQQEIDAARKAAADRKLKKTQGREWDWEKTEDDWNRSRPYTDSMTNPNPGKHSWNNQRGSGFRGRPARGPHPRGRGAGRGGRGGARGQGGPESQKADQPSAHDNQSGPVQGQSASNGNDAPPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S8N1</accession>
    <name>A0A5B0S8N1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007907</name>
        <name evidence="3" type="ORF">PGTUg99_030923</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S8N1"/>
    <dbReference type="OrthoDB" id="2497590at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138297" evidence="1">
        <location>
            <begin position="24"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="201" mass="21776" checksum="07FD439F54EAC0CB" modified="2019-11-13" version="1">MFITKSSVCASFLSFTVLTLVRATPFPSDTSGIPSNGPFLATRGLGSALISGSSYGPLHKRESHFRRGLPVNMGKEIQNLKNNMTDQSNRLSVLHNMALPKSLKPLQAKDVNQKIEDILTIGNYMKDFCKAIVAAFPNNVEANNAMNDIENKGKIFGRALTDSKRPVGGDGRQLPGNIEIAQAALVPVQNARNVLIRIASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SD21</accession>
    <name>A0A5B0SD21_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003899</name>
        <name evidence="3" type="ORF">PGTUg99_034788</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SD21"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="63" mass="7109" checksum="EFC68DE3CA46B242" modified="2019-11-13" version="1">MHSSPAALKFLFNFGMCSSRILFEFLRSKFDLRLAAVFFGLRGTPLTPNSSSRDSLAYFQGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SN68</accession>
    <name>A0A5B0SN68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013731</name>
        <name evidence="2" type="ORF">PGTUg99_030065</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000002">
        <property type="protein sequence ID" value="KAA1138583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SN68"/>
    <dbReference type="OrthoDB" id="3344688at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1138583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="102" mass="11054" checksum="D5786E6F07C147E8" modified="2019-11-13" version="1">MYASSSLSQFTEKPGMSHWLERSQCWKYLKAREQKAARPPSTSRYGVTPTGPATLADPMAASQEAARAGGVVPGAQSCGLLNWRKPCTEADLTPHHDLLPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMY6</accession>
    <name>A0A5B0LMY6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027947</name>
        <name evidence="3" type="ORF">PGTUg99_017659</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000142">
        <property type="protein sequence ID" value="KAA1128382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMY6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="84" mass="9395" checksum="6082C073D6195620" modified="2019-11-13" version="1">MAKESAKRKHWQEEAKKKQQEEEAKKKQQVEEAKKKQQENSSAAASGSTSRGPAESTNDSESDGLEEISKAEFKKKMANSRPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LR79</accession>
    <name>A0A5B0LR79_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012019</name>
        <name evidence="2" type="ORF">PGTUg99_017738</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1066942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LR79"/>
    <dbReference type="OrthoDB" id="10268827at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366030" evidence="1">
        <location>
            <begin position="21"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="132" mass="14892" checksum="C63D21D727C1B41B" modified="2019-11-13" version="1">MHFSILLLFLAVSLIHQGQAESSAPDEKPYPVRNCEEEDRFILLCARPVKPEDNLKTAEGLMVEKAPLIPGHERRLCNILPISGSMATRGYCCPLNPYGRPDNAGAKPEFQFKLEKDCKRVRVNWPKMASKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LTA5</accession>
    <name>A0A5B0LTA5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LTA5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="173" mass="18985" checksum="8447F97743BFFBC6" modified="2019-11-13" version="1" fragment="single">KAGQPITSISNRYRQPLQIPNSSSLFDRIFHAFSTGTDSIIPSVESSTVIGQDRLTVGRSASRRLIGAVSQTKLSHFRRDPKNELPNRSINRPCINCSDPASRVFKRVQAIDLGFECASPHHPCGIDPASRGFPSVSAVLTQFARMSGPVFIGFADPSRTPSHISYLLNRNFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LU39</accession>
    <name>A0A5B0LU39_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome b5 isoform B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CYTB5B</name>
        <name evidence="7" type="ORF">PGT21_022714</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LU39"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF14">
        <property type="entry name" value="CYTOCHROME B5 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1067952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="84" mass="9477" checksum="9771A8A8A530E8F3" modified="2019-11-13" version="1">MSEDKELPKLSTPSNFHLVISGKVYAVAKFLEEHPGGDEVWLTEAGKDATESFEDVGHSEEAQMLMEQFYVGTCKQRDLYNSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LW30</accession>
    <name>A0A5B0LW30_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012127</name>
        <name evidence="2" type="ORF">PGTUg99_015432</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000207">
        <property type="protein sequence ID" value="KAA1123569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LW30"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1123569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="77" mass="8524" checksum="4051E9FB70EBA0E8" modified="2019-11-13" version="1">MAIPPFYIHAPRCSRAPQLGGRFEGFHRGCWGLCHFLGYIKTSPSDSQIVLQAPSAIESNTQLAYQQFASLPFKANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYR6</accession>
    <name>A0A5B0LYR6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mini-chromosome maintenance complex-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028018</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYR6"/>
    <dbReference type="OrthoDB" id="329666at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019140">
        <property type="entry name" value="MCM_complex-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13489">
        <property type="entry name" value="MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13489:SF0">
        <property type="entry name" value="MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09739">
        <property type="entry name" value="MCM_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="62903" checksum="083654D92AE8AC63" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZK0</accession>
    <name>A0A5B0LZK0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000178">
        <property type="protein sequence ID" value="KAA1069935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZK0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="114" mass="12502" checksum="14AA27A2C93AB720" modified="2019-11-13" version="1" fragment="single">QKLSCEIGLSRNLVVSLAKNCPAKSAFQEISLFHCKAKNCPAKSALSRNLVVSLAKKLSCEIGLSRNLVVSLAKNCPAKSAFQEISLFHYTNFKICRAKNCPAKSAFQEISLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZK5</accession>
    <name>A0A5B0LZK5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZK5"/>
    <dbReference type="OrthoDB" id="3142841at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052979">
        <property type="entry name" value="Adenylate-forming_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="257" mass="28611" checksum="F103830EF861FDF6" modified="2019-11-13" version="1">MSASIREVNEAAECDETSHTLLRGTSAIDQGNVDTAVVNPRAESLLQAENQAWNEDEETVTVCMPTEQNSKPKLEGYTAPKLNRWITILTWFTPYRQLFVLTFLVNFIGVVLALAKVWPWPKNQAAPLVVGNILIAIAIRSEWVLRFLYWVTVKTFRPKIFPLWLRVKIVGILYHIGGVHSGCGLSSLLWLAVASSNQFLEANLHHAIPSVALSICLACTTLTCLSAFPSYANAITIFLKSFIVLWAGLEFYPQSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3Y4</accession>
    <name>A0A5B0M3Y4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3Y4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="123" mass="13926" checksum="4D038DDE8A2E4DD4" modified="2019-11-13" version="1">MDSMNNLLWRPSFESVGVTHLSPGKQSDLLASPSKPFGRHIHIPDSRLMNEQKSCGTKNQPGFLDCSMEVMTAVDDDRPTTHHRPNQAAMRALIDWLTSWHFALYSVSIDSQEPAKDFLEMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5B6</accession>
    <name>A0A5B0M5B6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5B6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="174" mass="19732" checksum="549B264AD8329657" modified="2019-11-13" version="1">MTNRDIAIPGPYGEEEEGICPDRRPTFYSRLYCYEHLIQVASWRQQFMKNSEYNLSITAKPRFLNFHEPSQKTINGVWNGPMTWCSSTVKARTTGTALPTLTDLNNFGKTSHSLRPFLTIHTVRLGERVRSLGMEFGRERVSGWESGHGGTAQGNMAIKRCSFKLLLLFLPCIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M810</accession>
    <name>A0A5B0M810_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M810"/>
    <dbReference type="OrthoDB" id="419616at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="8608" checksum="821FD238CE65A7F5" modified="2019-11-13" version="1">MAEITDESNKARAFPILPLCWAIGLMIGPAIGGYTAEPAKQYPHSIFARHLLRPLSFGGTLQRLGCRPRHTVSGGDFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MA03</accession>
    <name>A0A5B0MA03_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MTF1_1</name>
        <name evidence="9" type="ORF">PGT21_019933</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Mitochondrial transcription factor that confers selective promoter recognition on the core subunit of the yeast mitochondrial RNA polymerase. Interacts with DNA in a non-specific manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MA03"/>
    <dbReference type="OrthoDB" id="16079at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="557"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="397"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1073645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="588" mass="66046" checksum="C799024C4E53D2A8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAL4</accession>
    <name>A0A5B0MAL4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAL4"/>
    <dbReference type="OrthoDB" id="2506489at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="141"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="380" mass="43987" checksum="4ED10429D94E5BB7" modified="2019-11-13" version="1">MAPGKRPKNLYQFHYRSQLPNTETRSEKKARLTCQKRTKAEIGDSIRRQINRRDTQDQNDQESTNPDNPDTETHAQESLGFNENSLWEDTGEYMNDEDSNSLARIRSLHEEFIQKQKHQHWKDVMDSMFPVYLHLKNQTADWTLPCALDNFSSLLCKCTPDKYKIREVDLIDLMVLLLANGYLSCTPIVPQTAFSIQLLNFYDLLWNFCNTHATPFCKVLQSWNESISIRLLAKNSKRPRDLRRNFVASIDAYRTLKSMQRKIIQTATSITKQEVLAQRSCPACFGVAVPIDNPIHPETQSTQDFDTNKVFICLDGNFQHRHHERASKNYLELENEPLFVTPEEIALSNLEIREGELAKRVSQKAIAAHNNTKLLTIVEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAM1</accession>
    <name>A0A5B0MAM1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAM1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="110" mass="12381" checksum="A9B4D8EF1E287B2A" modified="2019-11-13" version="1">MTAHKVYSGLESRNDRSIDSSQEGTMEGMTTPPHLRTFAVTGDGVCITQSCHLQGLSENREDGCCNSFSVPKNVHPHEAQSLALSQWQFASWFHKVSYRRRPDLINFNPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MG55</accession>
    <name>A0A5B0MG55_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MG55"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="56" mass="6539" checksum="C6E988A487482688" modified="2019-11-13" version="1">MVPTKSNFVLWNHRQKEHSVRGTNNKIGLTKHLQSRSRGFHGSAVICRFHNPTWLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGG4</accession>
    <name>A0A5B0MGG4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026498</name>
        <name evidence="2" type="ORF">PGTUg99_007829</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGG4"/>
    <dbReference type="OrthoDB" id="2507215at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="252" mass="26909" checksum="716E867577A056DF" modified="2019-11-13" version="1">MSSTPSSPLDPHGPLTDQQSRDAYMNARRQLPREIPPATEQLRPARREQMDLSHLNLSDIPLPGATSHEPPMTPISRWLRNIEPLSPTTDLALRTESYSSPESDGSMDTASTSSVVADRNQQSPTRIIAFTREGLFIPEGISPTNARHRPADIHPIAGPSNARMVSPSPAANSDSDSPVLSSSPAKKSSNSAGMVSSSPTKKTVKVNGKGKAPGRSDGKKKHGVPTANLPRRPVVKIPKSRLRSGKGPASKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHY5</accession>
    <name>A0A5B0MHY5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHY5"/>
    <dbReference type="OrthoDB" id="2506139at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="215" mass="24236" checksum="A801C90FB21F2163" modified="2019-11-13" version="1">MDPQNVPILTKAEKVIQICDAIHHLQMTPKKFINAFLTNADPQIAYKRRFWGTSTGWSSTHGIIQAVKAEVARTRDGADRWGQLIENESVEILLSQSPPRGNFPLGSFHSSRTVQPSFFDPKAVSERDKALTKNHMPFLFNLIKRMLVRVEAPNELDEDGPVEPTIADPEQDHILNMDNVTYVADRNSAFSIQIHKACFSFGFVQVEANHQQPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML34</accession>
    <name>A0A5B0ML34_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010232</name>
        <name evidence="3" type="ORF">PGTUg99_012803</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000348">
        <property type="protein sequence ID" value="KAA1098656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML34"/>
    <dbReference type="OrthoDB" id="2509353at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366168" evidence="1">
        <location>
            <begin position="19"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="487" mass="54061" checksum="C8190469E36A4C31" modified="2019-11-13" version="1">MLKVYCLLAISFGILVAGQPKEHRSVHPVDPSYAPRRQLAQTEPAYNISLYYAEAGYQDAAHYASIISWTMRYPTVVAWDHPGIRSINCDLNGIHLEFKSQGFKLKAQQWEFPLVIVLDGDTGHCVSDGPSQERYHPFYVESVLQGHEEEPITLNLSGYRSRWDVVAYEHQVQVVEVKDKQSDSDSSRKLAHKRRGVMAPTTMQVSPSLNFDHQEKKCSNSEVPINVAQWPYGSLDIKCKNCFVDSDFQLIIDWGSRIINAGIECINQLIKNLYRAEQQLIALARSAENILVSQFSKLSEKLVDLISGFYNRINEIALSANHTQKVEVKAELAEVVSSYEDTSSKLLKLAQGQLATGIISAPASPQDTFVATHKLKEAISSTSSELGLMIQNGTQLVNDELSLPLCTTSPRKEGGGRCRRPPKRTVARLTGRLKANFDVELILTGTGEVSNGDVNVLLLNLPGLLIPGVSSLSPSSGWVRKKHLTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MM95</accession>
    <name>A0A5B0MM95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MM95"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="127" mass="14792" checksum="B435C4C8E784F8CC" modified="2019-11-13" version="1">MSHLIIRGGNFMEEVFKWRCKSWSGMPQTDSFRTGFRPRVQLHNQIEDLGALRPSSLQASSQVTKCTFFRPASSRPTFDLIRLDQAYMMQTREQTTKSSKNCVRCPRFLLISYIPITFMQNDKASFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MN82</accession>
    <name>A0A5B0MN82_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MN82"/>
    <dbReference type="OrthoDB" id="2503459at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="157" mass="17502" checksum="1A5C2D77FBB74C02" modified="2019-11-13" version="1">MARSSVTPRGTPSRLPSRQSTRIVTPTASSSYVRPSRDLRRSVGPTSQSQQKGDSLDSESEAESVVQIPSNQQSKKKRTRKPPSTKQRKRARSSITTNTDDDSDEVDGVIDHTQDSDNENSKVQGSSKKKTSNLDNIREYFEAPYHAEQTDEDKVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQB2</accession>
    <name>A0A5B0MQB2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_030981</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyltransferase superfamily. METTL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQB2"/>
    <dbReference type="OrthoDB" id="433955at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF164">
        <property type="entry name" value="HISTONE-ARGININE METHYLTRANSFERASE METTL23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1078224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="516" mass="57274" checksum="1314DF94764AC1E6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRI7</accession>
    <name>A0A5B0MRI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRI7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="97" mass="10664" checksum="FE29871AC0DF7B39" modified="2019-11-13" version="1">MIFFLSFFFDQICRVGPDPTLPDPNPNHPKTGSDSGSRIPFLSRPDPPRDLCRVGFGHGFSARTRPTYTLKSSQLCEDRQPGNKMNAPLVKALTSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSC1</accession>
    <name>A0A5B0MSC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSC1"/>
    <dbReference type="OrthoDB" id="2499250at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="259" mass="28832" checksum="2785B5938AB0C3DF" modified="2019-11-13" version="1">MAKEIAAIIRQKNGDFNHYIDNNPQCFCHVLALILGAGLRSLKLQNQIQPPKSKPDFFPKLETIEQVVEPDGVYVDLTEDVDVQPQEINDLDDRDEVDPDDASEGKLSEDDQEKTPTDPTDKASKNGSTAGGIGHTLVKVDYISCQICSSTARRAKFKLVSQNIGYKGPQLIPGYGIRWNVVYDSWTQAYAAQKVIVQLLNDESDKYARKSAAGHFFKGYEVSNKEWQDLNSLNQVLEADLVSGNKKEVNRILCPSSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUZ0</accession>
    <name>A0A5B0MUZ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006564</name>
        <name evidence="2" type="ORF">PGTUg99_021321</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000244">
        <property type="protein sequence ID" value="KAA1120464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUZ0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="94" mass="10711" checksum="E4A88E3866042527" modified="2019-11-13" version="1">MYNFQINAIRDDAVLGKRSLKSGEIHCSRTKLGTPGRRICTWGITSSAYTHQIQRIPQAFASSLSTRRDKRLPAVDWQNPIDYGSTYVNLRKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYC9</accession>
    <name>A0A5B0MYC9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="10">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_029453</name>
        <name evidence="12" type="ORF">PGTUg99_028379</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="10"/>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYC9"/>
    <dbReference type="OrthoDB" id="1732691at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000004">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000005">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825:SF44">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="SUBGROUP IIII AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1081104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1131266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="463" mass="50910" checksum="A884C398F6CCDB68" modified="2019-11-13" version="1">MLVNPAVSTPELARLPAGDFHERTFLTFYIFCFKTTTSMFSSAARPIGRLSHSLCTRKPSGSLDITQFSSTIHHPFSTCHRNLSQSANTTGIEASNLVIKTTETPRTKLPASELKFGQTFTDHMLIVKWTRPNGWKAPEIKPYGNLEIDPSASVLQYATCLFEGMKAYKSNDGKIRLFRPEMNMKRMNQSARRLAFPSFKGEQLLELIKKLVKLEGEWIPTEAGHSLYIRPTIIGTGAGLGVGPPTELTLFVICSPVGPYYRTGFKPVSLLASSKYCRAWPGGSGAFKLGANYPTGFLPQMEANSDGFEQILWLFGENDNLTEVGTMNLFVALEDKSGTIELVTPPLDDKILPGVTRCSVLELLRNHLDGSQRLDGLPPKFKLSERPITMIEMLEKSKTGHLKEVFGTGTAAIVSSVERIGYKGKDIHVPVGPGGLGVFANVVQREILGRQTGEIPSDWAVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1L8</accession>
    <name>A0A5B0N1L8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1L8"/>
    <dbReference type="OrthoDB" id="10412180at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="91" mass="9973" checksum="F7475986C961032D" modified="2019-11-13" version="1">MTSSLEGPERHPDPTLCPNHLVSSTLELKKPERPAAHRVLNIVPAMMGSSRMTMQRFTNLSRESKCHLNGPGPNRPTNHQDPNHLGAVTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6I6</accession>
    <name>A0A5B0N6I6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-chaperonin molecular chaperone ATPase</fullName>
            <ecNumber evidence="2">3.6.4.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SSB1_3</name>
        <name evidence="11" type="synonym">SSB1_1</name>
        <name evidence="13" type="synonym">SSB1_4</name>
        <name evidence="10" type="ORF">PGT21_025582</name>
        <name evidence="13" type="ORF">PGT21_034154</name>
        <name evidence="11" type="ORF">PGTUg99_006498</name>
        <name evidence="12" type="ORF">PGTUg99_027412</name>
    </gene>
    <organism evidence="10 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 15">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6I6"/>
    <dbReference type="OMA" id="NIPPMQA"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000014">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000004">
        <property type="entry name" value="Heat shock protein SSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000005">
        <property type="entry name" value="Heat shock protein ssb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1084362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1092250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1109620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1119794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="635" mass="68959" checksum="6EE9B1F0B05EF286" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8H7</accession>
    <name>A0A5B0N8H7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026725</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8H7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="294" mass="33760" checksum="BF8DF2314E763AB8" modified="2019-11-13" version="1">MDELDTLASHQLRHQAVLERLANCDYDLGLPDTPSDLSIDKLRSKEYLLIELHSALLPLLRQHITSLSPALRELNQAQGKPTPTLKLVIEILLKLELTLDQTIRTLNDLIPGKLPKPSRTNDQHFKEFKCFRLRGFERFIKRVMQAQLATFFSKSRQLIETFTLPDQSRTPVTTSSTKAILSIDFTIVWLKGSELYLIYTNTWVFSLEKIDTTWDTLLAVADPSHPRHIELSRSFKPMVKLSKLFFKKIATEGMTNNMAPIFTEMSSFQLDLLGTTAEKNHRITCCLSVFIRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAP4</accession>
    <name>A0A5B0NAP4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032924</name>
        <name evidence="4" type="ORF">PGTUg99_020263</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NAP4"/>
    <dbReference type="OrthoDB" id="5424058at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="287"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="764"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1086375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="785" mass="88538" checksum="2753517CA6635254" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBG3</accession>
    <name>A0A5B0NBG3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_004054</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBG3"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050973">
        <property type="entry name" value="H3K9_Histone-Lys_N-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223:SF3">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SET-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SUV39H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Pre-SET" evidence="10">
        <location>
            <begin position="616"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="9">
        <location>
            <begin position="722"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="11">
        <location>
            <begin position="933"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="235"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="382"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="422"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="479"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="821"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="303"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="353"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="450"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="479"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="833"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1086577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="950" mass="103887" checksum="E58EECE6605CF4C3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND34</accession>
    <name>A0A5B0ND34_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pH-response regulator protein palC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_024363</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the palC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND34"/>
    <dbReference type="OrthoDB" id="10266451at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071467">
        <property type="term" value="P:cellular response to pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037505">
        <property type="entry name" value="pH-resp_palC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40463">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40463:SF1">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1087177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="441" mass="48412" checksum="37BDF73F4171903D" modified="2019-11-13" version="1">MSYLFDLSTPATINFEPLLTDPTGERIGQLAGANAARGAMRTSLKECKQNSGKRDWFKAVKTIEDYLPHLLGLMDAIEKDMLLMPTEIVFGWRSTLSSRRVRSPSRISLPSFYYELASVLLNLAFALSNTSNSLVSSMGSYEIGTEAERRAGDGKLSSAADTLCRASGIFEFLSKEIIPKWEKHHREGIAHLRASRPPELSREATGGLAQLMLADAERLAIRRLLSRSAMDRHTNPGAGLPKSHPSPALLAKLELNVHQLYSSAKDAFKLASGASEIASSLRNYVSEGRQVALGRGYKWLALEAGEQASQNGEAIGWLGLAREAMKAVCSGSLLAVRNGSLKNDWARKKGKVEQELEEIDKFLKAYEQLNRTVTFEPIPPEATLLTRVPGGRAALSMKTYTMPSPLIKDFGSYTNPSVLDWADSDDEDLPSSSREPEQAYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDR6</accession>
    <name>A0A5B0NDR6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDR6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="136" mass="15094" checksum="BB374F640E10F620" modified="2019-11-13" version="1">MFDSLRLDSGAKHIQQSHKPLLTLRALERFHQMADVNRILDVQSKAGAIEPLGIPNTYIEGVRLLQEEVHGKPSELGRFKLHLPFDEQCATPPSDPEESPLLDSDQSPDIEEDEISSASTEGSMSGSSMDEDMTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGM1</accession>
    <name>A0A5B0NGM1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dynactin subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_003864</name>
        <name evidence="15" type="ORF">PGTUg99_019481</name>
    </gene>
    <organism evidence="15 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="15 18">Ug99</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Subunit of dynactin, a multiprotein complex part of a tripartite complex with dynein and a adapter, such as BICDL1, BICD2 or HOOK3. The dynactin complex is built around ACTR1A/ACTB filament and consists of an actin-related filament composed of a shoulder domain, a pointed end and a barbed end. Its length is defined by its flexible shoulder domain. The soulder is composed of 2 DCTN1 subunits, 4 DCTN2 and 2 DCTN3. The 4 DCNT2 (via N-terminus) bind the ACTR1A filament and act as molecular rulers to determine the length. The pointed end is important for binding dynein-dynactin cargo adapters. Consists of 4 subunits: ACTR10, DCNT4, DCTN5 and DCTN6. The barbed end is composed of a CAPZA1:CAPZB heterodimers, which binds ACTR1A/ACTB filament and dynactin and stabilizes dynactin. Interacts with ATP7B, but not ATP7A, in a copper-dependent manner. Interacts with ANK2; this interaction is required for localization at costameres. Interacts with N4BP2L1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Stress fiber</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the dynactin subunit 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGM1"/>
    <dbReference type="OrthoDB" id="283815at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008603">
        <property type="entry name" value="DCTN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13034">
        <property type="entry name" value="DYNACTIN P62 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13034:SF2">
        <property type="entry name" value="DYNACTIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05502">
        <property type="entry name" value="Dynactin_p62"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="503"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="587"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="645"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="613"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="656"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1088367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1105354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="679" mass="74747" checksum="AA2E3D53C639AFF2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NH74</accession>
    <name>A0A5B0NH74_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NH74"/>
    <dbReference type="OrthoDB" id="1434354at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="31"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="340" mass="38318" checksum="29277ABAD79C1A2D" modified="2019-11-13" version="1">MITQCLQWRHQFEGIGIDGLYEELDPFDFPNRDQVFKYWPIYFHGIDKVGRPVNIQMFGSLDLSKLYSVIDKQSHFKVLVANCEALTREILPACSHRNQAINLQNSSQSDHDHHSQANSSSSHSGASPKITNAFCIVDLKGFTLTQFWQIKNIARTCFSISQDYYPETMGYLAIINAPKSFATIFKAVTPWLSKETISKINILGEDYKSTLLEHIDDENLPSFLGGKCQCDNQFSCSKNDANFDRSPWLKERNWKSQSWKKFNVQTPAQAQDRNPSPQADPSASVSKSNDPKANDLQTHPPNNTPKNLPEQQNEITDGGDTSDGALIINNSPKSPTKNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJX8</accession>
    <name>A0A5B0NJX8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJX8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11667" checksum="88FCAD7CF05FD5A6" modified="2019-11-13" version="1">MRPRHKLRSPEISAMIVYDDHGQPNEALIDSYRFRFHLNIFEPFKPIGSSAFSDSESRASLTALLYNQISPPTIATSTTSRTITTTEVGAENLSERVIQLDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMI5</accession>
    <name>A0A5B0NMI5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-like F-box</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMI5"/>
    <dbReference type="OMA" id="FFDMPNQ"/>
    <dbReference type="OrthoDB" id="4540290at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022728326" description="Cyclin-like F-box" evidence="1">
        <location>
            <begin position="25"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="43188" checksum="3F5155ECEF1B00DA" modified="2019-11-13" version="1">MIRISFLWTLFLLAFLSKTSQSRAFLNRRQRGAGGASPTFKINSEQAVAQAQLAQTKGVVAGQASNEKIVPMTAEIGGFAIQFKVSGDPSDLQPAAKNGAVIRATETTAGDAAAGKGINLLLHGDGGQSFFDFPNKGDQVGLLGVVALAPNDKMFWGGGGGNQRTDGVLHSKLLDALITNVLPQVIKMDSSKVFFMGISGGSFLLAGFFLPAFAEKYQSGAILGCGALPPQVQPSANFGKTLETMRIHFQTSAQEQDGIQVTIPQAIVSYLKAATDAQVDKKALAKKLTADATPKGTHCVFDGKQFVSGIQLLTDNYSRVIFGDGDIKGIGKVSTSAADNNQKFASGVPVAKLRNNAAGGVPQGSQLRTAAKGFPQGTQLRSAAGVFPQGTQLRSAAGVFPQSPQLRNAAVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NRI1</accession>
    <name>A0A5B0NRI1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Dyp-type peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_035309</name>
        <name evidence="11" type="ORF">PGTUg99_036618</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="11 14">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DyP-type peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NRI1"/>
    <dbReference type="OrthoDB" id="3207336at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049509">
        <property type="entry name" value="DyP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048328">
        <property type="entry name" value="Dyp_perox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006314">
        <property type="entry name" value="Dyp_peroxidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01413">
        <property type="entry name" value="Dyp_perox_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521:SF4">
        <property type="entry name" value="DEFERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521">
        <property type="entry name" value="DEFERROCHELATASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21105">
        <property type="entry name" value="DyP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20628">
        <property type="entry name" value="Dyp_perox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51404">
        <property type="entry name" value="DYP_PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="DyP dimeric alpha+beta barrel" evidence="10">
        <location>
            <begin position="10"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Dyp-type peroxidase C-terminal" evidence="9">
        <location>
            <begin position="296"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1076163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1091533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="54656" checksum="71C9E8FCDEF13965" modified="2019-11-13" version="1">MSSNKVDLKNIQGDIIIGLQKRVEAFWFCTLKSESNSVEGFRRNLKDSLLPLITTTQDVLDTQKKINDHKRNKRSEGGNDLLPITQVNLGFSFAGLKKLGLKLEEIPTGGDGVFSKGQKVDAVDNLGDPVDPKTKKLKTWSKDFLHEANSIDFVILITAPDCKLLDQKLDQLRKNLSGFLASSFVRKGNVRPGNEVGHEHFGFLDGISTPTIENINAQPGDKKSGIVKPDVVLLGQPASGGNTNNLDPKQAWIKDGSFMAFRELQQLVPEFQSFCDESAKKLQNPNVSGDFIGARIVGRWKSGAPLTLTPVQDKPELNRAQDFDYSDELKQERCPYAAHIRKTNPRNGIAGADPNTAVLPHLMIRNGIPYGPELTEEEKKTKKTKENRGLLFVSYQSQIESGFQFVQKLWCNNTDFPAKTPAKVTPGFDLLIGQTADEKPRVAQNINPLGIAGTTDPNNIVTAPAHFIVPLGGEYFLMPSIKAINEKLGLSSRSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NS44</accession>
    <name>A0A5B0NS44_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019795</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NS44"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1090980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="149" mass="16059" checksum="FB0AE888CE31A404" modified="2019-11-13" version="1">MSEMYGLVDFEAAFVFGGPSESLSHHIAKCTWTKVQDVRPCGRIFRNISERTSEMFRTPAPVVTGIRGRAVPSVELGIAHVIPFLLGHSSWAKLEASGFYVEPSQAGDGTRGMCLISPCQSTRNRAIAAVSIISSRPGSPSMICSFQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTD0</accession>
    <name>A0A5B0NTD0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000082">
        <property type="protein sequence ID" value="KAA1091952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTD0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="123" mass="14427" checksum="03B716F600F92001" modified="2019-11-13" version="1">MYPSKRKETLPMDEVHVPRPSGRKPFRWTRYMYSSSGRKVFRWTRYMYLVQSRNGKAFLLDRGSMYRRSKRNGNPLPCLDEVTIVPRSIAEGKAVPLEDVIVVETTPTRENLEAWQGILDIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUY7</accession>
    <name>A0A5B0NUY7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003522</name>
        <name evidence="2" type="ORF">PGTUg99_015101</name>
        <name evidence="4" type="ORF">PGTUg99_018670</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000410">
        <property type="protein sequence ID" value="KAA1086439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000312">
        <property type="protein sequence ID" value="KAA1105152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUY7"/>
    <dbReference type="OrthoDB" id="2495772at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137565" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1105152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="10750" checksum="D1DB13A7E41D0FEF" modified="2019-11-13" version="1">MYSVSFITLAILALLGQLILANPDSTPRETMQCSNYNGANTKSATCDDLPDVTCIGGCSGTPAVAEGCQVSDGSDPEHKVPPSKQTCDVGFGRDTLASKCKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWE0</accession>
    <name>A0A5B0NWE0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011815</name>
        <name evidence="4" type="ORF">PGTUg99_002336</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000377">
        <property type="protein sequence ID" value="KAA1092128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWE0"/>
    <dbReference type="OrthoDB" id="2498157at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="136"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1092128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="982" mass="112684" checksum="B31F1C10D6FE85D2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZ08</accession>
    <name>A0A5B0NZ08_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZ08"/>
    <dbReference type="OrthoDB" id="10271750at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022726259" evidence="1">
        <location>
            <begin position="24"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="203" mass="23758" checksum="B2B1BB33125BE605" modified="2019-11-13" version="1">MHSRRRIVEILVLCCLLTARHMAQVAPCQLKHARVGEDMTGSTSSQTRLSNRESLDLLPQISDIPAERDEENTRRQFGTSVQALQNQSKLEGRFDRKRCQEATMKEDFKNLHRQLQIFTEAAKGVYKEKNIPDLLDSVVKLVRIYNRIPTFKHDQVLKWEDKILVLLPQKPNDLYRIDEPPTEIRPQDDIYQGKEYVLLIQFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P332</accession>
    <name>A0A5B0P332_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000962</name>
        <name evidence="3" type="ORF">PGTUg99_003600</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000069">
        <property type="protein sequence ID" value="KAA1095573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000041">
        <property type="protein sequence ID" value="KAA1135081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P332"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="196" mass="22624" checksum="2279D807724CA0EF" modified="2019-11-13" version="1">MLLSKILVAFQILHYYDISAHPLSLSNHLVKRSEKLEDLIRELKKETRLLYDSAGSSANHPGETRNSGKKENAEQAYKRLHDDIKRLKRENDDKNLKLEKILGELQTSGQKYWDENTCQKISALSDILKNEQKKINKTAQSLPPLEHTKGQKYQSENHIQNLYNLMKTVNEGEGRKTAILAKIDDYLSNHHFLSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4R2</accession>
    <name>A0A5B0P4R2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4R2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="170" mass="18722" checksum="74A2FDBB27996C08" modified="2019-11-13" version="1">MVNCVHIDTLSLDLKPIDADGRPSSDVVSIRLHVHPTRLIGKRGRLDETRNTANTSSGMRLKSDGKNGTDLCRAVPLQEPIPVDRPNQCALMLMPHQIGENVDITSLRCPSQSFSSDPQIVQQKDADRGEDFEADRPAGDWTETVDSLLPIGKPSLWRTKSNQSLQSVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8B8</accession>
    <name>A0A5B0P8B8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_030408</name>
        <name evidence="9" type="ORF">PGT21_032189</name>
        <name evidence="8" type="ORF">PGTUg99_021618</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8B8"/>
    <dbReference type="OMA" id="VDHRRKW"/>
    <dbReference type="OrthoDB" id="30343at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040107">
        <property type="entry name" value="Snu23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="6">
        <location>
            <begin position="97"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="176"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1096860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1107999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1113468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="235" mass="26610" checksum="711D576A63767F36" modified="2019-11-13" version="1">MSNKKQSTYGAPAAGTDFRKTWDKAEYEAKAKQRDEEERERMKEADEALQKGKKPRRKHVDLPKPTELMKQREAPLELNKNQNKTMIVQGSSRGPGAPGFYCDVCSRTSKDSSSYLDHLNSRFHLRQLGQKTQVARSTIDQVRAKIAEMREATKQQAASKQYDFSQRLKEIKAAEEAEREAKRLKKLQAREAQAKAKPPSDVYQPAPESNEINGENGADEMAKMMGFSGGFKARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9B8</accession>
    <name>A0A5B0P9B8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9B8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="79" mass="8373" checksum="C7BDFF3D56950CE8" modified="2019-11-13" version="1" fragment="single">MTGLSKSEPTVPTSLPSSTGSWYFCASKKTTAKPTAAVQGPRLESCNKTSVCSHQETCSSTPITQSIQKYVVETKLAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCZ3</accession>
    <name>A0A5B0PCZ3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035992</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCZ3"/>
    <dbReference type="OrthoDB" id="10566628at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="148" mass="17452" checksum="B3B7401444E9BD39" modified="2019-11-13" version="1">MWSGIQQLSHWLSKKFKLATKRRRETHEQLENLLSKQNPFKQPGCKYKVAFFQKQWNHQRTFRADHTDEEQERRDKLIKIYEHQGTLASLRERLLDPELLLLPEKDIKKNVKAIEKVAAKLGKEAEGVENLPSGEENSIMLFFTLHTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PDF1</accession>
    <name>A0A5B0PDF1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Importin-alpha export receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CSE1_6</name>
        <name evidence="2" type="ORF">PGT21_003733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PDF1"/>
    <dbReference type="OrthoDB" id="3266000at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF8">
        <property type="entry name" value="EXPORTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-2 central" evidence="1">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="387" mass="44069" checksum="C432D63040E5C79B" modified="2019-11-13" version="1">MFEDDSAEFVRRDLEGSDNDTRRQAAIEITRALIEQFQKEVTAIITRYVQNYLQQYAADPTGNWRLKDAAVSLLASIASRSSTTAGGVTNTNALVDVVLFFSEQVVQDLSVKNSAPPHPIIVADAIKFLHTFRNQLTREQLISQLRFERILFMKRNNQLLFSSTDLKDLAPELLTGLFAQIRKGTTPQQIAQNEMLMKCVMRVIVTAQKALLPHCEVILSNLIMILTEISKKPEQSKIQPLYIRKHFCADTMLELPLQSFRTLFQTLHDLLPPLLTPTLWELSWETFPALVRLLRVTSVPVLPGSLPEKSYLSYAWSVPKVDWLKAKRLHAKQTLFDSQRRSNCHYPPKRISSATYPAQQSKTDKFTIGFLYTIMFIVTLEKASVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHK0</accession>
    <name>A0A5B0PHK0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="3">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PIK1_2</name>
        <name evidence="9" type="ORF">PGT21_029472</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05168">
        <property type="entry name" value="PI4Kc_III_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000016">
        <property type="entry name" value="PIK1p Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000015">
        <property type="entry name" value="Related to PIK1-phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000039">
        <property type="entry name" value="Related to PIK1-phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057754">
        <property type="entry name" value="PI4-kinase_beta/PIK1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049160">
        <property type="entry name" value="PI4KB-PIK1_PIK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21245">
        <property type="entry name" value="PI4KB-PIK1_PIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="8">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="7">
        <location>
            <begin position="1261"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="310"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="443"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="545"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="660"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="764"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="816"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="904"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="968"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1026"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1124"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1166"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1220"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="339"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="380"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="443"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="816"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="916"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="994"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1132"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1224"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1235"/>
            <end position="1247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1100100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1634" mass="178232" checksum="1A191BE729BFB09C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHR3</accession>
    <name>A0A5B0PHR3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Pheromone-processing carboxypeptidase KEX1</fullName>
            <ecNumber evidence="15">3.4.16.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Carboxypeptidase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Pheromone-processing carboxypeptidase kex1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PGT21_009234</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.6" evidence="15"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHR3"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000121">
        <property type="entry name" value="Carboxypeptidase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF190">
        <property type="entry name" value="PHEROMONE-PROCESSING CARBOXYPEPTIDASE KEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="527"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="552"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="620"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="627"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1101155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="666" mass="73514" checksum="F937EC4D3E124195" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKI9</accession>
    <name>A0A5B0PKI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKI9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="50" mass="5766" checksum="CA5D3D5DFDA51D35" modified="2019-11-13" version="1">MLSKLKNSTPPSGGYQRKLVDSEWDHILSLYNFLHTPTPDTRPEFPTLVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PL05</accession>
    <name>A0A5B0PL05_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028809</name>
        <name evidence="2" type="ORF">PGTUg99_011828</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000035">
        <property type="protein sequence ID" value="KAA1136245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PL05"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1136245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="107" mass="11491" checksum="EE6EA71B2F046B71" modified="2019-11-13" version="1">MQMNTPKKTSAKTTCSKTMNKSLALETHPMTRAVSPQGIEATETAVPTLIEGSQDSGGDDSLDLISKNPELTVETLQEIAEKTAIPPESELSATVEMVKDKICASRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRZ6</accession>
    <name>A0A5B0PRZ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRZ6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="131" mass="14837" checksum="A55FF42C5437C9F7" modified="2019-11-13" version="1">MLISPDFQTPFYENSEILRVLTHVPGGLVHIIDDQSRRPTRPIRQCWTPWADVGEITLSFTFKDGDERLDRPGSFTLLTLLWTGDLFGRGFPNPESRRGLPDYLTSSVPSRRASTGHGSTNSFIRQVVCRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSS8</accession>
    <name>A0A5B0PSS8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032108</name>
        <name evidence="1" type="ORF">PGTUg99_012304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1098983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSS8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="63" mass="7080" checksum="5895275C2F8B610B" modified="2019-11-13" version="1">MGFLEQICIIVIPELLPEEITSRLDQCAWIMNSLKLTASVFESDGSLLIYDTCDLSPNYLLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTQ2</accession>
    <name>A0A5B0PTQ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTQ2"/>
    <dbReference type="OrthoDB" id="2499604at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794:SF2">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="206" mass="22737" checksum="EAC6CD771DC7F725" modified="2019-11-13" version="1">MFMNTNTIPIALIQSLSLSLEKLKMNSLDTPDKELGRAFSYLAVYSLLGTLLRWSYGVKLLQPSEQKGNDKRELKGSYAINDCEKVEFPSSVGHDCSMFHVEDTAAPQFVDLAVPTRTQGAYRISCATAFTSESDLGTPSKPSVRFSYPTAFGAYSNLLGPNGSKKEYTRMSSATAFSVESEITHCDITGEIYKERRKSCRNGPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUE4</accession>
    <name>A0A5B0PUE4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017192</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUE4"/>
    <dbReference type="OrthoDB" id="2504952at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17756" checksum="D8483A041D9C0126" modified="2019-11-13" version="1">MLLADFYWLLDKLRGELRQDLQGRGEPLSVEAQVAVGLYRPGHGASYVKIGQLFNVAKETANKALDLFVNAVLKVLRKQAVGYPPIDRTDEWDEIKASFERYWDEQTHHKQLYGEVPQGHENGKKDQQTNVEYPQVYMWTRRDIIRDYLYTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVG5</accession>
    <name>A0A5B0PVG5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030276</name>
        <name evidence="2" type="ORF">PGTUg99_010992</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000414">
        <property type="protein sequence ID" value="KAA1086058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVG5"/>
    <dbReference type="OrthoDB" id="2509522at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="53765" checksum="65EC5065242C70EB" modified="2019-11-13" version="1">MDPSPSRQPERSFLSELPTEIKMRLVEFVALQTELRLYSYYHAFMKLALVDRTFYELCSPSNWRELDLQFIDYPDIDELVQEILPPQAKHVQSIIIAMHPDDRQVYEWDSDEIVEAEQMFPESEYNKIFRICTNLTKLTIRLQTTSLSLDRWGDFELDPLNPITVLLQPISQLSNLTFIDLSHQYRQQFEETFLVELIKNMVNLVHIRLDKVKAAFPTCDFCVCEYSKQPFQSPLAIHLASLSSLKFLHFHMVDSFDSGWSKLGWKGALEGISLGKSDVSVRALHSFCKLFEDSLVYLCLFRAPSSLQKRGRTQFLLDSERDCIFRLPRLKRLSVSNQYMTEFLKLFYESPNIIRICTMNYSRDLISFEDLKSFMNPDDPKWIHLRSLMVHIRQNTFSENDIKELIRYGAEAGVEVECGNLRGVRPPFFEGDLFPYELGSPDWSDAESDWEVDSEDEDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZ02</accession>
    <name>A0A5B0PZ02_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030058</name>
        <name evidence="3" type="ORF">PGTUg99_018103</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZ02"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137883" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="16284" checksum="8FD0AE085B373CAA" modified="2019-11-13" version="1">MMKLTLLVGMVTFLSITPSHAFRCDNAFDLHGPPTRGGEERVACTTGSVKRVCTLDRCTVRGPPGTNDRYLSDLLFIRCVDKAHPSHSFSVAPVNYHIHNDEQLDVWGYEVYRDTNVRVGQLSAYLCPRRNQWRPECDDTACGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1Q5</accession>
    <name>A0A5B0Q1Q5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004874</name>
        <name evidence="3" type="ORF">PGTUg99_014803</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000010">
        <property type="protein sequence ID" value="KAA1137266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1Q5"/>
    <dbReference type="OrthoDB" id="2500693at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="545" mass="59268" checksum="066303C545AC9DD5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q575</accession>
    <name>A0A5B0Q575_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylinositol-4-phosphate 5-kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MSS4_4</name>
        <name evidence="2" type="ORF">PGT21_010273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q575"/>
    <dbReference type="OrthoDB" id="20783at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="387" mass="43077" checksum="A0B76E1A2FC4CB82" modified="2019-11-13" version="1">MEQQQQPIHNQIIINHLTNNSNNNSNNNSKILQQTTTTTTTTTTTTTTTSTSTTPPNNKTLEQQQQQQQQQEEEENPPSPSPTSASSLGSSTHQYIPTPSLRIHHNDYHEQTSTPHPSSTTPQHKNRSTLAPISTTRPTTPLSLPEPVHHTPSSASFQAIQPTYERAYSSATTPLTRSPSHCSLQRMPTQPPLARLAPSRSNEDHLAPIPVPLLPENNNHLSPHDLDHQIARHAEVIRKQRQSRRIEHVHESVDSSSLSYEPPQPPTTLPHHPPPPLSSSSTNIYPHPPPITTTTMNTTTQQRLSSSLDHPHRSNRDQNKRRVSGPNNDVHAGVLVGNLIGEGHVNYVLMYNMLTGIRIGVSRCQAKVRRPLTDADYTARHKFTFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6X9</accession>
    <name>A0A5B0Q6X9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_028411</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6X9"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.60:FF:000006">
        <property type="entry name" value="Predicted mechanosensitive ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058650">
        <property type="entry name" value="Msy1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323:SF11">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25886">
        <property type="entry name" value="Msy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="564"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="590"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="511"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="367"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="781"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="781"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="805"/>
            <end position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1108891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="853" mass="95997" checksum="33FF8E2403E228B9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q732</accession>
    <name>A0A5B0Q732_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025336</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q732"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="73" mass="8350" checksum="40AB1E58ED9C307E" modified="2019-11-13" version="1">MVESSTPIEINPQSSHCYRIEECTPDGKSASVPSWAAIKKLTEIPKPVKRRWERLGMSRLKMQGLRRPTVLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q778</accession>
    <name>A0A5B0Q778_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q778"/>
    <dbReference type="OrthoDB" id="203796at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37848">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37848:SF1">
        <property type="entry name" value="SUN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="485" mass="54957" checksum="1A98A02D23E1BC50" modified="2019-11-13" version="1">MSKVQSSTKSDGHHIHRHSSDSIDSDEPPRYSDEPSGSSAATYQQQSSALDPSLSGKSVEPSGFFPTSSAPCPLAGDLEAGQSANDTQPPEFSVYQASYTADKLGNITSHDSHLNTDGEALCRFILLHISSPPELIVKLSGTHEDLRTETTTEYVNGRTEYRTREQRQTVTDFSFTMQAAAEPALDHGIRRLYIVGDGEVVGRGRHRRERGRWENGKDLGLLNQLANEHESSDLPGRGRTRVGFKERRKADKIADETKRFGYPPFVPSWMFPRSAFSCEPSRPPDHLLGPHWSDKCLNYFNSVVRRGYYRHQKPNREESAKVERELRQWCDDYCANNKYLKELRVEKAVFGWNLALLEEELLKLLNSTPHLSSTHFSVDFYTTSSQILVRPVNFVSKIFSLSGFIKFILSITLVYPILWLMRYLILGAEYDVVRVAYPLVYWHEPEHSNAEARLIGQSELDWFSANQANIARACQNSKIGSLQSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9X8</accession>
    <name>A0A5B0Q9X8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_002691</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9X8"/>
    <dbReference type="OrthoDB" id="21204at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46077">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TOPORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46077:SF1">
        <property type="entry name" value="TOP1 BINDING ARGININE_SERINE RICH PROTEIN, E3 UBIQUITIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="31"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="99"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="99"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1109909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="287" mass="33116" checksum="4825D1984451D275" modified="2019-11-13" version="1">MHSTFEAICNRNTQTNIHEDAQEGGDNQDQCAICLDLIINQAVIVPCQHSEYCFRCMRIWTTTSNRCPICVQPIDHLVHRIDPRTKDFQIFHPLPIMPNLNNGDDNNRQSQAASPTLPAAQHPPNSESGPSSSQSDHFIGLDRRQFIYRHSLYVKHIPSNRYTQFYPVSSKHFLKDRSKNPSEPTRRDCPSAKLIARTIRFLQRELRAIQPEVLAHPIEHSIRVIIIILHRADSNSSEAVHLFSQLIRDPQLAQHFSHELTCFLRSPYDDLLQWDQVLQYPTAPNVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA95</accession>
    <name>A0A5B0QA95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009636</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QA95"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022737924" evidence="2">
        <location>
            <begin position="22"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="847" mass="98474" checksum="7F095DD275C344DC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAA1</accession>
    <name>A0A5B0QAA1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAA1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="10425" checksum="D4897B3C96D699E2" modified="2019-11-13" version="1">MPTCEAGHLSGPEAARLVGPLRGRGHRFGGMLRAKRDLDRETLPGCLPRARLCLGSGAGPDQAQHGSIRSITAPARRRGTLPPMSHRPPAPARGDFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAB0</accession>
    <name>A0A5B0QAB0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4-nitrophenylphosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001883</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAB0"/>
    <dbReference type="OrthoDB" id="413953at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1109893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="17599" checksum="AA4C84D2D6B6B30D" modified="2019-11-13" version="1">MSMVKSLKSHKDLQGFIDRFDNSLFDCDGVIWHGEELIKGVRTVLELVRISDKKLIFVTNNATKLNILPSLSRLSFAPTFRDEIFGSAYATALCLKRILKFPDNKKVYVIGENTDPANNEFIDHMDFSSIQTDKDVGAVMCGFDMHIKQDIPIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB30</accession>
    <name>A0A5B0QB30_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_018249</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QB30"/>
    <dbReference type="OrthoDB" id="2496846at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1110329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="523" mass="58857" checksum="C8F8BA325B7B3A0F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
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<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFA9"/>
    <dbReference type="OrthoDB" id="414698at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046452">
        <property type="term" value="P:dihydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046655">
        <property type="term" value="P:folic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069:SF3">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00070">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="8">
        <location>
            <begin position="44"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1111835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="293" mass="33497" checksum="1426B710DA062D6E" modified="2019-11-13" version="1">MLLVSIQDLCALKFIREKVNQPQPTRIHKYHLPIIRAKPRGMIPLNLIVCATKSNGIGQAGRLPWRLKEDMNFFKSVTTLAPSGRKNVVIMGRKTWLSIPSKFRPLANRINIVVSRQSKDPAALDIHQQQDSYLVNSIESACHLIRALDSPDNNTEQETTTSVLKERKTGGERLVNKVFVIGGSEIYKSVLDSQPDNNRLYRPSTILMTRILSEHPAIETSLDAFFPEFRASKHWSKSQNPDLLNQFLVLPDHQNSSGSVPEPHYQLPFNFDQLIIENSFMYKFELWTYNNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFC0</accession>
    <name>A0A5B0QFC0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFC0"/>
    <dbReference type="OrthoDB" id="2519178at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="275" mass="31283" checksum="688065AC69229835" modified="2019-11-13" version="1">MGDKLLAKGVSKNTKHHSPQANILVSGNSASKWWRLRRELRDFLSLVTGFKWTRRCPAFPRDALPQPIRFLKFCLLNFDSADRSDKCESDSKSALPNAQPLALAGILVLLIMLQEFNWHDPPKLDDILNPLETSQIQVSHSILPDSTTPLASASYSALHHSKASNDATSNLSTNHFNPDHSFRDSFERFYHGKSIVNNNVTPDSEANWWKDFEGTFGEHHQNSLTFPSTSTLHQTIQENHTEPEYLPYIQPEEIIEPTDYNPTGPYEVTEMHKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFC6</accession>
    <name>A0A5B0QFC6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFC6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022815555" evidence="1">
        <location>
            <begin position="22"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="331" mass="39255" checksum="F01E1296B2FEAF7D" modified="2019-11-13" version="1">MKYTEIRWFLVTASLILRCWCGLISEPQMGQVFKDVYKKLSIKDVTWGSRARRNSVDHHINKLTDKLNELGENGRERILHMIVTNLEEILNGEKLDLEESWRRYMIDHRFTEKEFNLHDAPQAENLRDTIYNWCLGENEAWSTLRPTDIEAWRLSKLKELKNFKVTKDTVRVAVDFHTFTMAWNSSFTYDAFAYALVISRSGYNWPDQKSNQLMEKIQKWSNSKCCMKFEEYKHGAENIIKNPYQDSGIQLQSRMGKYDRDSCHINILDNFMKFSLMLDLSFLDEETPEFKSILHLRQGEFINLTLANLKKIKTKPTLQVNYHFPIREENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFI6</accession>
    <name>A0A5B0QFI6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Exosome non-catalytic core subunit rrp46</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RRP46_1</name>
        <name evidence="7" type="ORF">PGT21_017629</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFI6"/>
    <dbReference type="OrthoDB" id="27298at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0271">Exosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="6">
        <location>
            <begin position="14"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1111967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="8014" checksum="15ECFAB928344833" modified="2019-11-13" version="1">MSIMRSDSRTEADIRSLTMRMSILSRSDGSAQFSFGDLKALGAVTGPAEVRIRDEKPTEAFVDVIVVPVCGLPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QG03</accession>
    <name>A0A5B0QG03_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1112029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QG03"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="186" mass="20657" checksum="053D07F690CA5A30" modified="2019-11-13" version="1">MLRGTIDGTTPRHIPVLKRRTYQHLQFTSVAPIPTNAVDMRFLNYLVFSVGVMPLVHAFDPHMQKPNFPCPDPEHPTRVIGFCIREPDPQELVDKKVNPDEYFRFMVNAPTTDSGFNCKDVNILGKPASTGQCCPANIPNGVVSTVIPIVIFISECVKILTSSPPQTWLVPVASVADYCTLKKIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJB6</accession>
    <name>A0A5B0QJB6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJB6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="137" mass="15294" checksum="E834CB3E87C932FF" modified="2019-11-13" version="1">MGDPPWPGARTPSEGQWAASCWWRTEDQRHPTRLGWVAQLTLQEIGKDSGAKFPLFLISSHHFLSPSFPKGNTRPFSLIEQCLRYDLATDDSGATSTDTPQSSPLSESVQAHRSFRRSTRTTCGTEYCEVLEGLEHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QK21</accession>
    <name>A0A5B0QK21_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH:ubiquinone oxidoreductase intermediate-associated protein 30 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_033285</name>
        <name evidence="5" type="ORF">PGT21_033375</name>
        <name evidence="6" type="ORF">PGTUg99_026443</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CIA30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000106">
        <property type="protein sequence ID" value="KAA1130853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QK21"/>
    <dbReference type="OrthoDB" id="426386at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010257">
        <property type="term" value="P:NADH dehydrogenase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013857">
        <property type="entry name" value="NADH-UbQ_OxRdtase-assoc_prot30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039131">
        <property type="entry name" value="NDUFAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194:SF19">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08547">
        <property type="entry name" value="CIA30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033847820" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30 domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="3">
        <location>
            <begin position="64"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1113537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1113546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1130853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="251" mass="27920" checksum="9414FDA86E82294E" modified="2019-11-13" version="1">MIVTHPGLCLFLFSIQLASLAPASLDIRRSNAPVVDDGQAENQDRAGTIKPFLVIFGSANRAWNTENWTEVSDTVRGGQSKAMLKYLDRSDRTAGVSLEGMLDTKTLGGAGFASQVYRHPISLPMADRYSAFFLEVEPVEEGHQGLVRTFTFGARDRPLGSPNESALTYQFDFQLPALVTVQRDSASTKLDQLVRISIPFNKLVPTFRGRPKSDEKPFDPHQITQISFMARSFFNHQSGPFLLNIRRLGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QKA4</accession>
    <name>A0A5B0QKA4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QKA4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="618" mass="72452" checksum="3471FE5690AD9CF9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPH7</accession>
    <name>A0A5B0QPH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Translation elongation factor 1 beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">EFB1_2</name>
        <name evidence="9" type="ORF">PGT21_033435</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPH7"/>
    <dbReference type="OMA" id="YRWYKHI"/>
    <dbReference type="OrthoDB" id="331763at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000001">
        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018940">
        <property type="entry name" value="EF-1_beta_acid_region_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049720">
        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001326">
        <property type="entry name" value="Transl_elong_EF1B_B/D_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF21">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10587">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01182">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00824">
        <property type="entry name" value="EF1BD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00825">
        <property type="entry name" value="EF1BD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation factor 1 beta central acidic region eukaryote" evidence="8">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="7">
        <location>
            <begin position="138"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1115206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="224" mass="24717" checksum="D55A390AC50491ED" modified="2019-11-13" version="1">MGFDFTGNDYSQLEQHVYDKSYIEGYAPSQADVVVFQAIASSPDVKKYPQAARWWAHIKSWESEHASLPGEKKELSSYGPAKGSTSAPAAAAGGDDDDEIDLFGSDDEEVDEAAEKLKATRLAEYAAKKANKPKTIAKSLVTLDVKPWDDETDMAELEKAVRSVEMDGLVWGLSKLVPVGYGVRKMQISLVVEDEKVSLDDLQEKIAEFEDYIQSSDVQAMQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ43</accession>
    <name>A0A5B0QQ43_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_029219</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="297643at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056458">
        <property type="entry name" value="TPR_DOP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF24">
        <property type="entry name" value="PROTEIN DOPEY-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24597">
        <property type="entry name" value="TPR_DOP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="8">
        <location>
            <begin position="89"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like middle TPR" evidence="9">
        <location>
            <begin position="392"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="10">
        <location>
            <begin position="1332"/>
            <end position="1838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1020"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1146"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1420"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1748"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1020"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1149"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1420"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1438"/>
            <end position="1447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1115004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1864" mass="209420" checksum="1507B7267310ED0C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QW11</accession>
    <name>A0A5B0QW11_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024689</name>
        <name evidence="4" type="ORF">PGTUg99_035586</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QW11"/>
    <dbReference type="OrthoDB" id="2503300at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1117093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="366" mass="40507" checksum="27147E6FEC66D639" modified="2019-11-13" version="1">MNALKLMGSISLVFGIILLLLPLLSTPVVKGLSFWRAVFEIDDLKIEVRTGFWGACTSYKDTSDRIGPDFKKGKKKPKGGADADVWVKCTKPKAGYSFSMSSTVPDAAGELSSTQTLFLFWLLLAAFLALISLALSFSPNYYQWKHAAILALTSSVLGFGTFLACVVIFTGLTRETRLTDSAKTIPIYGGLQGCTFFPLSACILLGIGAGLLWMSFRQEFFKLHRRSNRPNENRISYKSETMPSLPPPILGNDGISRDEWFQQPLESRHGTEIPREAHVNARRPADDEVPQENSMDHELEEIQSPSQHPSQADDEMDTETQSQYSMFQSDSLGSASLSYFNSSDGLPPPPPLQPRRSTRARPTKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWV1</accession>
    <name>A0A5B0QWV1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_019116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWV1"/>
    <dbReference type="OrthoDB" id="2505311at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="221"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="454" mass="50868" checksum="50090A5E7DB226D7" modified="2019-11-13" version="1">MSEVKPFVGSNELETHNGPEPNIPGRTAGSEAKLPTATNEVEGSDAASKQNSQAPGETSDSESKPHPEEEEPRAKPKRRQREYRKRKKHENRAKKNKTGRENRLNQVISSKSLCPLVGHAEKKLQKFDLFPQISDEIAIEKERRLKQCGENSSDNPNQVFQKPTDPPLIPRAPTETENNWALNHVQENFLNLDHGRVMMYDLNNDKQIVGVVEYLKIPEMTPNQRKDFDFLCAFFHGCRQFVLPAALEGRVCADITSAIGWSKETTHLEILRRYQNKEAIEQNQAVYSKLMEGSARAAAILWGLFYNLGDTAAQNTRNHLKNLLGSSPANDETHQGSTSSLAFPSNGFHNEYSFEKEDLSQHPLSFAMMIPIFKSTGKVGLESEGYQVDNGQFVFPDIRIGIKFPPDTVCMILLRDQEYAHGILNPTVAGDSTRLGVCIKPPAKLSNTSIKQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYS8</accession>
    <name>A0A5B0QYS8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029438</name>
        <name evidence="3" type="ORF">PGTUg99_003955</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000153">
        <property type="protein sequence ID" value="KAA1127708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYS8"/>
    <dbReference type="OrthoDB" id="2500274at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366549" evidence="1">
        <location>
            <begin position="21"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1127708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="113" mass="12896" checksum="017341DC7A5B247F" modified="2019-11-13" version="1">MFFLGSNSLLLPLPLEPSVAQPIAVNSIIQRSLHRHAKVELGIYLLLDSEPTESNGNQSSLSIHGHQFSSSTEDYWNDCSETSISSDEFIPAHLYTPRRVPITQLIKNWWRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYU9</accession>
    <name>A0A5B0QYU9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tryptophan--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Tryptophanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">WRS1</name>
        <name evidence="14" type="synonym">WRS1_2</name>
        <name evidence="14" type="ORF">PGT21_011022</name>
        <name evidence="15" type="ORF">PGTUg99_001175</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000249">
        <property type="protein sequence ID" value="KAA1118476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYU9"/>
    <dbReference type="OrthoDB" id="10261385at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000003">
        <property type="entry name" value="Tryptophan--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000033">
        <property type="entry name" value="tryptophan--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="537"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="544"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1112799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1118476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="560" mass="63079" checksum="F01192AECD89CC1B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1D3</accession>
    <name>A0A5B0R1D3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SURP motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1D3"/>
    <dbReference type="OrthoDB" id="2552978at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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            <begin position="293"/>
            <end position="305"/>
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    </feature>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="360"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="48305" checksum="90E1107951DED04D" modified="2019-11-13" version="1">MLQPASLKRQRPSPSPPHHNSNNNNNSLHQHYHHQENPSDQNQERALIRNLRALEAELCDLNRLALQSESRCDQLQGGRLIRLNSNIISNQQDNSVWVDRYDARLLLSPPPTSTTDSNLEPQITSPPPASPTGYSDLPSDHEEMFYFEPEERQEIAQKKLRRRRDDERSVRIKLREEEDRLREEELRLSKIPPAEQITLMKRTLDALKGSPSPSLLEIRILTNHGNDPRFSSFLRRDGKWRAYWEAMKAGPDPSDSAAPTTSSSSSTQPSDPQGSSTPHPTSNPVVAAGGLVDYDDSSDENENDDLVLSKPLNLEETEPPADNDPSSSDNPIEPRGETNNDSERAPESEAQASLQTTQPTLPIDDDDDGEEEDILLNQLQPSTLLDPIPTPKTVDPIERLNRQQRAKLWAQNRKKSAPVRLEPALPTTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1U4</accession>
    <name>A0A5B0R1U4_PUCGR</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        </lineage>
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    <reference evidence="1 2" key="1">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Visser B."/>
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                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
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    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <dbReference type="OrthoDB" id="10284060at2759"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="14656" checksum="34711EC3D0F4CA3B" modified="2019-11-13" version="1">MDGQSRHRLGGSCVFERIPKKLCITKNKKSTQQTNLSFSKTGQAGLHSLYFYFCPNHQLKYYLLYSRYKARIGSTTNDDPPVIIKLGSFTSIDIKETLHKLIVNPFPIIRGNILSIHQNSTDVVIVLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R232</accession>
    <name>A0A5B0R232_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="71" mass="7586" checksum="466EE30439A5FF18" modified="2019-11-13" version="1">MFSWDAKFRRLGMTHPIGALLAGGTDQSGWRPLCPSPLMTRSFCSQLMAGSRLASFSSGADFCLISASVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3K7</accession>
    <name>A0A5B0R3K7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036996</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="KAA1120020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="192" mass="21755" checksum="B83D6EFB4F31F62A" modified="2019-11-13" version="1">MYARTRRGGNRWRRLPVGLCSVFRFVYVTLQPQQQSLIKANLLLKNNFNAVTAANCQLTSLIINFSPTIAYRSISRSHLRMRFSALLPGFVVFLIKNQATSGQSINGRFPCPDEYPAHFCGRETTPQEHATTRYVGYLQPPKWLGNHYTCVGVIAQKKPAKFQFCCKNNAFGNRSVYNVGITAEDLAEKRCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R468</accession>
    <name>A0A5B0R468_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_033379</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF36">
        <property type="entry name" value="FI03272P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="3">
        <location>
            <begin position="445"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="193"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="254"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="507"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="728"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1212"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1337"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1388"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1039"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="357"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="411"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="806"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="844"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="871"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="893"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1229"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1346"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1390"/>
            <end position="1403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1432" mass="151790" checksum="762DF380ED8DDC12" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R5Q2</accession>
    <name>A0A5B0R5Q2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynactin subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_023472</name>
        <name evidence="9" type="ORF">PGTUg99_027948</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynactin subunits 5/6 family. Dynactin subunit 6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1121031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R5Q2"/>
    <dbReference type="OrthoDB" id="2355at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027777">
        <property type="entry name" value="DCTN6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13072">
        <property type="entry name" value="DYNACTIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13072:SF0">
        <property type="entry name" value="DYNACTIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474705" description="Dynactin subunit 6" evidence="7">
        <location>
            <begin position="18"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1105894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1121031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="159" mass="17596" checksum="7E16446EF31845D2" modified="2019-11-13" version="1">MTIFWLILDCIIHPSCTIVALGQIIYESGCIVKEQSVICNRVEAISICSRNVFEAKSWVSPEVQVGSDSVIGAGTTVVSEPDLLYWFENARDPNEEDEDDGRQQSCLLDSLVICSLPDFTVIYGSSWVQSTWSGESIGQAIGLHTKHLLHLVKTSKDVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R817</accession>
    <name>A0A5B0R817_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024856</name>
        <name evidence="3" type="ORF">PGTUg99_023845</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1120974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R817"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474573" evidence="1">
        <location>
            <begin position="18"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="592" mass="69411" checksum="A1BCCD0C9393506A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RDK6</accession>
    <name>A0A5B0RDK6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035772</name>
        <name evidence="2" type="ORF">PGTUg99_002677</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000212">
        <property type="protein sequence ID" value="KAA1123005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RDK6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1123005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="84" mass="9200" checksum="1B227AF8C667E64F" modified="2019-11-13" version="1">MLTDPAVSGFTDSRHVEKSHSAQVLNHFGATDFPDNFIYTSPYDVSQVGRPVEMGKPELVNLTPQCAMWSSLKKASPTVMSTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RIW0</accession>
    <name>A0A5B0RIW0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023179</name>
        <name evidence="2" type="ORF">PGTUg99_006430</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000175">
        <property type="protein sequence ID" value="KAA1125720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RIW0"/>
    <dbReference type="OrthoDB" id="10276735at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1125720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="410" mass="46633" checksum="E6F62AF43F957559" modified="2019-11-13" version="1">MAPKRKTSAGKKHRKQGDRVVEGFERLIKKFDPTAAGNILRSHVHEPFQIDQSEVKRDLLHDLRSNLLPQLGGQIINLLLLLDPNRLREDPGGHLKLILDIQSELDQTLSRIESATFSYPHATEPLFDSIEFKDQDLKEIKWFRLDGLYWRINGEFLVVVTRIFLESNELLQQMKLSTAEFHDQPDIDYTREQIVYYASSSLEGIELAIEWLDGSEFDLVQLEWPSEKDGFSENMDDLLSLTNRTSHLEPGNITGESAPGDDPAIKLSKSLLPVFKLCRLLFKKLSKRGLNKEHLPLFTKASSVELEHVAEMGGVVSYGLRNLLSVLRSVDRARDRLAHPDGAMMAAKIQDHVDTAVSLIAHYFVPLIPDSDTLPIQNHYKDWLATWSSQFRLAIRNFLNAALSFDEGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RQX7</accession>
    <name>A0A5B0RQX7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027765</name>
        <name evidence="3" type="ORF">PGTUg99_018892</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000142">
        <property type="protein sequence ID" value="KAA1128386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RQX7"/>
    <dbReference type="OrthoDB" id="5374349at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRM" evidence="1">
        <location>
            <begin position="22"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="115" mass="12224" checksum="31F03745F1F7A6A8" modified="2019-11-13" version="1">MESTPPAPRYFHAILPWPVELSNLSPGTTAAELCTALASYGNITHSFLLVSKMANLGAIVHFSDHRTAAAAAADLDGTCADGFILQARLMSHKEQFTFSCHLSPPPPPRCKFGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RU90</accession>
    <name>A0A5B0RU90_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013413</name>
        <name evidence="3" type="ORF">PGTUg99_031249</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000136">
        <property type="protein sequence ID" value="KAA1129596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RU90"/>
    <dbReference type="OrthoDB" id="10334540at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138189" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21269" checksum="74A2677A603DCAEA" modified="2019-11-13" version="1">MKSSCLAIFLALSASAYGYSGCFDYFLLKDGCVNAAEPSDLRCQKPSAPKEQHTTYAFKRDPSALVSPTCKPEISNVDYDTFPAVCLWTGPDWDNPKPEAGWVNHANPSNCGKVVYIQKKDQPKTRILAKVIEGCWFQGWFHGNYTTGCSAIAVSTAVMKKLAPTHVMDHMYIGPVVWDFVKNDGHQEVSGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1X9</accession>
    <name>A0A5B0S1X9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030998</name>
        <name evidence="3" type="ORF">PGTUg99_026079</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000105">
        <property type="protein sequence ID" value="KAA1131023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1X9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="54" mass="5933" checksum="C1B6BECED57905E3" modified="2019-11-13" version="1">MLRPRPRNPAAPSAHPTQAHFHLFPPTQAPFETESSTESSTAVPTPSDHLYGCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S2C7</accession>
    <name>A0A5B0S2C7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_014523</name>
        <name evidence="10" type="ORF">PGTUg99_036558</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S2C7"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000009">
        <property type="entry name" value="stromal membrane-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF1">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="515"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="8">
        <location>
            <begin position="838"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="56"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="495"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="700"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
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        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="756"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="780"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1096378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1132057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="952" mass="109055" checksum="DBBF2EA1D71810D6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SAP1</accession>
    <name>A0A5B0SAP1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027178</name>
        <name evidence="3" type="ORF">PGTUg99_009990</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SAP1"/>
    <dbReference type="OrthoDB" id="2497263at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="442" mass="49412" checksum="D4EB3D40BB94DD67" modified="2019-11-13" version="1">MSSETFGLIAQGFDDLVARCWKIDDNQSRIVPAESFSTDDIDAGKAHLLQIQTSLLPSLRQQLAGLMTSLKPGALEKDPIPKCNHTLEIIQKIGHILHQINASVNSFVSMPPDEGKKDQDYGVLKKFRCHALVEKINSLIGGKLRQLFLHYSVFIRCWEFPNDRPTFSSDDKSHRVELEERTLEITALSLQGIDSIIEWSNRSDFGILQDAWIPQVRSLSSNLAMVTRRLNPTVHPREEHDTTGGSPPGSTNSSGARENSQGDGRDGRSEAEVSSAHEEDQSRNNPEIPLTSHQVELIQQTIPIIKLSRIFFNKMISTRKGKPPFTLNSQISSADIASLQEEMVQISVCIADIVDLLCNANSPENLDRDTVELLPRWIQELSGHFDSALIMLGFHLVSTTSHPDPPQHESFFKTGFYSLRSQFRVALNNLVVSLHKHVPPSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SJI8</accession>
    <name>A0A5B0SJI8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_022898</name>
        <name evidence="7" type="ORF">PGTUg99_007385</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000009">
        <property type="protein sequence ID" value="KAA1137323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SJI8"/>
    <dbReference type="OrthoDB" id="10250130at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228:SF2">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_4G14350)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138380" evidence="5">
        <location>
            <begin position="41"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="524"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="562"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="627"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="941"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="973"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="489"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="656"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="696"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="828"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="911"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1116"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="705"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="760"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="911"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1124"/>
            <end position="1139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1067956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1137323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1202" mass="128533" checksum="074D0C2B025F1CFB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LIN1</accession>
    <name>A0A5B0LIN1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_014268</name>
        <name evidence="7" type="ORF">PGTUg99_009697</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000515">
        <property type="protein sequence ID" value="KAA1064135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
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    <keyword evidence="5" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1064135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1092796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="241" mass="26705" checksum="8E6BAC6FE17C08EF" modified="2019-11-13" version="1">MKMGYIRHLITQNVDRLHHKAIGVKQDSHAPDYFKPHPNILELHGTLKHVNCLSCGHQIDRDDFQDLLSELNPSWKNYADTLQSIGQEVKTNPDGDADVKDKSFDSFVVPNCEKCTVGLLKPAVTFFGESLSKEAKELANRRVSEATNLLLLGTSLTTYSAFRLVKELKEKAGSQIGMINIGTTRADELIDWKIGSHLDTGGLGCSQVLKEVGLQLAARQRLPDSHPVIELLHSGVMKPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJ88</accession>
    <name>A0A5B0LJ88_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_008489</name>
        <name evidence="3" type="ORF">PGTUg99_010482</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000518">
        <property type="protein sequence ID" value="KAA1063963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
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    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="511"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
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            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1063963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1065727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="771" mass="85707" checksum="9714F79EC4E9CD28" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LTB5</accession>
    <name>A0A5B0LTB5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_014058</name>
        <name evidence="5" type="ORF">PGTUg99_029147</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LTB5"/>
    <dbReference type="OrthoDB" id="10263751at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42032">
        <property type="entry name" value="YALI0E30679P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42032:SF1">
        <property type="entry name" value="YALI0E30679P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="347"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="418"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="499"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="297"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="424"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="538"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="559"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1067672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1093580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="600" mass="68291" checksum="90F9C7BB5B4D1639" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LW31</accession>
    <name>A0A5B0LW31_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ferric oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_018420</name>
        <name evidence="3" type="ORF">PGTUg99_034448</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LW31"/>
    <dbReference type="OrthoDB" id="3142841at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052979">
        <property type="entry name" value="Adenylate-forming_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="62918" checksum="AE9E6691BC784630" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYQ3</accession>
    <name>A0A5B0LYQ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_016248</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAMAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYQ3"/>
    <dbReference type="OrthoDB" id="2505655at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000022">
        <property type="entry name" value="Related to Dynein light chain 2B, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="3">
        <location>
            <begin position="50"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1069206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="149" mass="16206" checksum="D10C968FE7DB9EDF" modified="2019-11-13" version="1">MPEMMAVQTTPPSSSMSTAKPGTTEGAGPSQPTPSTTMPMTTSMAGPPEVESTIQRLSEHRNVRGVIILNRDGVVIRSSGPVFQGSDGVIVLRRYASEARKIVDAVGSSVDRMELDDQLRFLRIRTQQHELLITPDSKFTLVVIQDPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZL0</accession>
    <name>A0A5B0LZL0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NIPA-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_021712</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZL0"/>
    <dbReference type="OrthoDB" id="165382at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF65">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1069343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="22559" checksum="B746295528E15B65" modified="2019-11-13" version="1">MLAVMLVTAVLQIVFLNRALQRFDSKHVVPVQFVLFTIMAVIGSTILYGDFRDLGSAQVGNFVFACGFIFTGVYMLTREGGPSRENEETADEPRKLRSRCRNSTLTPVPEESIDSSDVVIQPADPSQSFAVGIQDPSSHADTLATKLASPTSWNSLQFSFSPPARRALRRQRSQPILFPRAFGTPGYYLIASSTSRSHGTSHGFPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M348</accession>
    <name>A0A5B0M348_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M348"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022950858" description="RING-type domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15218" checksum="B38FC58CFAAB0D74" modified="2019-11-13" version="1">MIPWLILLLLGNDCCWGSLGESSHIAFSHQAAESFPEANQLHLPSAPIERNMPLAFDCESPANQLGALSPDSTRDEQNPQLLGIVIDKQEGHCAICLENVDLVADSSKIHHCAPTARAQMQEQKKWAMSSLAPKQVNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3H8</accession>
    <name>A0A5B0M3H8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016979</name>
        <name evidence="2" type="ORF">PGTUg99_024603</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000174">
        <property type="protein sequence ID" value="KAA1125954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3H8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1125954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="107" mass="11505" checksum="2514F8C42069652B" modified="2019-11-13" version="1">MFGGSANRPPLPVQFRRRTIPWSGLPNAQSASESVQPVFCSASRLARKITAPSQPVSESTTTDWLAGGLGFTSHLVLVQSPKIMSGRPGRAELLAAHYFVTKRKGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7P9</accession>
    <name>A0A5B0M7P9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031050</name>
        <name evidence="3" type="ORF">PGTUg99_003594</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7P9"/>
    <dbReference type="OrthoDB" id="2513615at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="178" mass="19191" checksum="736C13A98099F9A4" modified="2019-11-13" version="1">MGHNPNRHAVVRPAPPNSAPAVVSTFPRSPPLSAFPAASPPFHLPSRLRPTAQVTRSNAIRRPVATHLNDIDINGDLVNKALGLTPTSPAPPLPSASIPPMKTSSLDYSTLPLPPLPLPPTPPPAAPLLPLLPFPRPPRRARVNSNRHAMIILVPQELPQELRDIQLDAWMTQDDDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MC32</accession>
    <name>A0A5B0MC32_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="5">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FAU1_2</name>
        <name evidence="6" type="ORF">PGT21_003439</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 8">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = (6R)-5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MC32"/>
    <dbReference type="OMA" id="DKWGIPT"/>
    <dbReference type="OrthoDB" id="2015992at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10420:FF:000023">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407">
        <property type="entry name" value="ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1074385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="291" mass="32612" checksum="4BF0FAD89C3CE86C" modified="2019-11-13" version="1">MLGRAVCQIRGTMMANPPSAAWFSSSAATHSSSKSKSTAYQPPPSMGRQKLLIRRQVQEVLKEITSESLVHQGEEVLKHLKSFNHYVDSDRVSCYKSMSSAELPTDSIIKNLLENKKKLFLPYIHSPSEPADPKKTGAHRMEMLELKSWADFQTGLVPVSFSSSSSSPQVFQFDPAKIAGLENALPEGLDLILLPGLAFDRFGNRLGRGKGYYDEYLNAYPSVASRPSITNYPDHLTEKQQSEKSTRRWPILVGMCLREQLLPEKEYIPTQAHDRRIDYLISPDGVLSLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCF1</accession>
    <name>A0A5B0MCF1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022311</name>
        <name evidence="2" type="ORF">PGT21_022637</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCF1"/>
    <dbReference type="OrthoDB" id="2514740at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="136" mass="15739" checksum="0430E565F1B19FA5" modified="2019-11-13" version="1">MDMNNQMYRPKNYYASSKLSMNDGSSSGSVPPHVSGMLSREQACEALLHPKEQQEGRTERLPPPNSSFNPRESFGQPNRTNMETWLSEMKVWELFFKENPLDNPPSRELQETTVRIVALMFNIRNTAFNHRPRPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ME37</accession>
    <name>A0A5B0ME37_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATG22_2</name>
        <name evidence="11" type="ORF">PGT21_001712</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Vacuolar effluxer which mediate the efflux of amino acids resulting from autophagic degradation. The release of autophagic amino acids allows the maintenance of protein synthesis and viability during nitrogen starvation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATG22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ME37"/>
    <dbReference type="OrthoDB" id="192733at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032974">
        <property type="term" value="P:amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17483">
        <property type="entry name" value="MFS_Atg22_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044738">
        <property type="entry name" value="Atg22"/>
    </dbReference>
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        <property type="entry name" value="Atg22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050495">
        <property type="entry name" value="ATG22/LtaA_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11700">
        <property type="entry name" value="ATG22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="470"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1074338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="572" mass="62004" checksum="16E8436FDA11396D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFN3</accession>
    <name>A0A5B0MFN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
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            <begin position="24"/>
            <end position="584"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="306"/>
        </location>
    </feature>
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            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="584" mass="67447" checksum="74E3FDF0B29B93B2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGL2</accession>
    <name>A0A5B0MGL2_PUCGR</name>
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        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="40"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="188"/>
        </location>
    </feature>
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            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="379" mass="42283" checksum="7903469979FD58E5" modified="2019-11-13" version="1">MVDQQHTLRNPLARLGEPTPSQIDGLPVELERELRLYGGLLIQQAGVLLKLPQIVMATAATLFQRFFFVTSFVHFGIRDVSAGALFLASKLEEKPSRIRDIINVYDYLLQMIEWSGEQVSSRTGTRVVVEEGIASRAAREAEQKIEQNPLLPLEIKQSMLRHLQQKRATLPSEPPSLPSSIPGPSGSSEKNLVLDIDKFSYLPMDYFAQRFYDRKEEIVVAEMQILKRLGFHVQVQHPYSAMVNYLQVLNLTDNSEISQRAWGALNDMLLTTLPALYPPTHLGTLAIYVAVKEAGLALPEAWWELFDVSEETELVEMAGILRAIYPPSATQPVDDGQNVDAGDGEKDGMAAGDPTIWVRVSRLPITKDAARRLIKPAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHC9</accession>
    <name>A0A5B0MHC9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHC9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="84" mass="9024" checksum="835692A57DF9EC5D" modified="2019-11-13" version="1">MASRSTTSKFEGSLSRPRLDVLCVICVVLVRPVLMCGVKASSFDFGGSWAQYIDQSGSLRATGSSSLGLDLPGPEQINLRSIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLX6</accession>
    <name>A0A5B0MLX6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLX6"/>
    <dbReference type="OrthoDB" id="2508734at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023056659" description="Hydrophobin" evidence="1">
        <location>
            <begin position="29"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11524" checksum="094C13C049CD92C6" modified="2019-11-13" version="1">MQSNIFLSSIPCMLLLVLIASLASYASAYHCEDIKPFPLAACMMTNYTTGELEGGGSRPGAPNRNDKFGCGDGFINTPLCCKIPFEKVDQKNVKDSCIFAPDGPARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0MMZ9_PUCGR</name>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
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                <person name="Schwessinger B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <comment type="caution">
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQ50"/>
    <dbReference type="OrthoDB" id="10267058at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213:SF5">
        <property type="entry name" value="BIFUNCTIONAL DTTP_UTP PYROPHOSPHATASE_METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="28161" checksum="DE5226046145953E" modified="2019-11-13" version="1">MMTSHPNISPFSGEASILAERALKLPLYTKLTGKRLVLASSSPRRLKILRDIGINPEVIPSRFEENLDHSSFEDPEFPEGKGLRYAAATASEKAIDVYTRLVVTSPDHPPDLVIAADTVLQTFFPGPGELPKILEKPDSPADQLSMLEELMNAPPETRVSAVTGVAIVYPTLASPGYTVKTLGETSRLWFGDVGRESLVAYVSQGEGIDRAGGISIEGVGSQLIRRIDGDWNNVVGFPAFAFVQFLKKVMAEDSDFLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQF6</accession>
    <name>A0A5B0MQF6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CxC5 like cysteine cluster associated with KDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_029426</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQF6"/>
    <dbReference type="OrthoDB" id="2505207at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041539">
        <property type="entry name" value="CxC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040898">
        <property type="entry name" value="CxC6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34305">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34305:SF1">
        <property type="entry name" value="SWIM-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18718">
        <property type="entry name" value="CxC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18721">
        <property type="entry name" value="CxC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC5 like cysteine cluster associated with KDZ" evidence="2">
        <location>
            <begin position="109"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="CxC6 like cysteine cluster associated with KDZ" evidence="3">
        <location>
            <begin position="325"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1078149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="652" mass="73987" checksum="D7B42195037DB8ED" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQL2</accession>
    <name>A0A5B0MQL2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003978</name>
        <name evidence="3" type="ORF">PGTUg99_032749</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000439">
        <property type="protein sequence ID" value="KAA1082317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQL2"/>
    <dbReference type="OrthoDB" id="10279445at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1082317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="295" mass="33307" checksum="E5F4C39DC93D3925" modified="2019-11-13" version="1">MDGSNLNAGSSPVQKSQFGHMTNASPSLPSRASRGSNLQTYSSGEVHHRPNLVPLETHIQTPSNKRNREDELFNNHPESRKPPETLDLLRDFGKNSAAGSPANAFETPRKAILSHRTMQTDGIQPPIQKEREGRFLSIDPTHTPHSYLKNLEDQLRSSVIKPMQMTPEASFRLDEMLKQTSYSDGTKQSDRTLVQIENVMEPKDTATEIVTISQSVNVIKGQTSTGDRLLRLEFAHEILRTHDPLLEDERIIEEMKKLNENSKIVIVESNFKNALDIIRNCNLMMKRDGPIELSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRY4</accession>
    <name>A0A5B0MRY4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4E type 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022571</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRY4"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF18">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E HOMOLOGOUS PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="432"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="60334" checksum="C44C57E4D1A6EC08" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTC1</accession>
    <name>A0A5B0MTC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008479</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTC1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="11157" checksum="DC04B3D848F4A3D3" modified="2019-11-13" version="1">MAFEAVAKVFNIPIAHVSRFRGLPFHTLPAKVEKHVQWLLFKHPTPPNTPRHLHPRSQGHKTSSSVHIFNLISARVDDAVAMEIGGPEDGKSYDDLNLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MV87</accession>
    <name>A0A5B0MV87_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_024637</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Su(H) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MV87"/>
    <dbReference type="OrthoDB" id="5600360at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000092">
        <property type="entry name" value="recombining binding protein suppressor of hairless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1450">
        <property type="entry name" value="LAG1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015350">
        <property type="entry name" value="Beta-trefoil_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036358">
        <property type="entry name" value="BTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040159">
        <property type="entry name" value="CLS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015351">
        <property type="entry name" value="RBP-J/Cbf11/Cbf12_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037095">
        <property type="entry name" value="RBP-J/Cbf11_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10665">
        <property type="entry name" value="RECOMBINING BINDING PROTEIN SUPPRESSOR OF HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09270">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09271">
        <property type="entry name" value="LAG1-DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01268">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01267">
        <property type="entry name" value="LAG1_DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110217">
        <property type="entry name" value="DNA-binding protein LAG-1 (CSL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RBP-J/Cbf11/Cbf12 DNA binding" evidence="8">
        <location>
            <begin position="390"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-trefoil DNA-binding" evidence="9">
        <location>
            <begin position="533"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="77"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="287"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="682"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="846"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="925"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="852"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="864"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="930"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1080885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1000" mass="109596" checksum="87AAC60770B3E97D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXE3</accession>
    <name>A0A5B0MXE3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002834</name>
        <name evidence="2" type="ORF">PGTUg99_026012</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000441">
        <property type="protein sequence ID" value="KAA1081651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXE3"/>
    <dbReference type="OrthoDB" id="10306778at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="146" mass="16998" checksum="566B65C92F895AC8" modified="2019-11-13" version="1">MQSQMQKYAMSRGSAPIVDWHSSSSYPTQLLQHAIPTRHQLLNTPYPLDTLFNTSPKKMEELLQFMDEDDDIIASLQTSIALIMKTQEKKKHGGSTPGRREIHWNRLEGHQQLYNDYFAEDAIYPDYLFRRCGILYGFPSNNFKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYG0</accession>
    <name>A0A5B0MYG0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYG0"/>
    <dbReference type="OrthoDB" id="2506204at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010816">
        <property type="entry name" value="Het-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07217">
        <property type="entry name" value="Het-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022911012" evidence="1">
        <location>
            <begin position="36"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="110" mass="11950" checksum="82EDF8068175754F" modified="2019-11-13" version="1">MVFSCTSCKPMSPSGMATNLFCAIVLISLTSSVSAFGSGNIPDAAYLKGKAWRHGDIEGDVFLCDIQRLSADQLNFKTDILNFCLATHRLIRGSCQSCWPGWGCNCRCDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N077</accession>
    <name>A0A5B0N077_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MTHFR SAM-binding regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_007369</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N077"/>
    <dbReference type="OrthoDB" id="16284at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000002">
        <property type="entry name" value="Methylenetetrahydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21895">
        <property type="entry name" value="MTHFR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="10">
        <location>
            <begin position="303"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="604"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1082577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="642" mass="72317" checksum="CFAEB0927A891381" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N436</accession>
    <name>A0A5B0N436_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019678</name>
        <name evidence="2" type="ORF">PGTUg99_030849</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N436"/>
    <dbReference type="OrthoDB" id="10323765at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1124165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="274" mass="29992" checksum="7E5223CEBCA54E76" modified="2019-11-13" version="1">MAGHSLTPSTNPSSKPPARSPKNFKCLYCFQKGHTISRCHIVTYDQQKGLIKRIGSEFWLSDNSPILLDPWRPIKDIVDTFSAIHNPQSFASLPPSTDFSYSLASTAATEESTMEEDLSKVTLSSAQTTEETNHQVVDNLPSLGSIGVKLNGYDCEGLLCPGLLFNFISEEEAQELDFDVHPQDIWMPGICDDDIKISGIALASLSISGVFETTSFLVTQKIGPLILGKPFMKDFSAVINYNEPEPPMLKVSDSGGNKHLFPISQATSNCTRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4X3</accession>
    <name>A0A5B0N4X3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_85</name>
        <name evidence="2" type="ORF">PGT21_026715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4X3"/>
    <dbReference type="OrthoDB" id="2507470at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="797"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="834"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="856"/>
            <end position="866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="866" mass="96411" checksum="30E190DCC91DBFFB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N582</accession>
    <name>A0A5B0N582_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025141</name>
        <name evidence="3" type="ORF">PGTUg99_010067</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N582"/>
    <dbReference type="OrthoDB" id="2500683at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="207" mass="22894" checksum="68D2D0B1A39ECDB7" modified="2019-11-13" version="1">MGFTSVCPASHTKTDRPDQGTGRPGHREGQTSFTCDEDGKTSANGIRPPGSMTNQPSTTELKTKDNSSSTALPKTAPGNDWSAIRKSWLQPNVKNDEPRPSSSADQAATTSNQLQKKNPKRLEKIDKMLADYDQASRLGHSNPKPMLSVVDFAFRLTDEYVRHPAPIPLSQAVSVLYRSWFHDGTIPEFHLEKMKDADDTEQEQLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5K5</accession>
    <name>A0A5B0N5K5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029805</name>
        <name evidence="3" type="ORF">PGTUg99_026376</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000342">
        <property type="protein sequence ID" value="KAA1099262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5K5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366214" evidence="1">
        <location>
            <begin position="21"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="180" mass="19943" checksum="D77EFA309B9588F6" modified="2019-11-13" version="1">MALSLLLVLIQGISLTVGLAVHVPPPAHNPVTTVGGGAVECNMPQSTFDYIAQNHRHYLGYKPYFEQGPMEDSDSETSEGKRILNKHLLRQGDSEEGLKISCLPDGAVNIKDNNYDCTIHPSHPLPKESWYDMAKGLIEITPFLARTVPPLMLYACVLPFESAYLRAKELVSSMKKPHEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDF4</accession>
    <name>A0A5B0NDF4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_004241</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDF4"/>
    <dbReference type="OMA" id="RITHSHF"/>
    <dbReference type="OrthoDB" id="2496822at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="9">
        <location>
            <begin position="269"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1086584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="440" mass="49638" checksum="78469B9FBC939257" modified="2019-11-13" version="1">MSGIWNNFVGLMPDKSILQHSTKSFFFLSLVLCAWTHPKYYHSRLARCLRVPLSLISATWWLCYPFLYLVEPRETKVSANIGNFAWTYVMMTKCLDWGLFSGPQHRRIYVRSSDDSGGAWEAAEKNLKDRLAPSSLIDQVLLQLTSMRGSQFPWGHFSKLNHNSLGCDMLKFLICHIYSLTVLAGLLYMEKLSTTSTIQSFVFFGRNVSLPTIVFMARFMSTPILLTGLCASFEAMNSALAVLAHLTHPLLCRLGFPDVICEFADPVYYPPLFENVFAFNSLSEFWGKTWHQLFRRSFVVLGAFPLGGMARALGFSTQAQKMAGFVGAFMASGFMHAYPFFLMADPTTSHGNVSRLEIFCVFLCFVVQSLGALIEPILIPLIPKRLGGGRLWTISFLLLTLPLFSMATNKPARLFSIFKPLDEWSIHYLFLPAVLTPIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NF45</accession>
    <name>A0A5B0NF45_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="13">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CNA1_2</name>
        <name evidence="16" type="synonym">CNA1_1</name>
        <name evidence="16" type="ORF">PGT21_019018</name>
        <name evidence="17" type="ORF">PGT21_023785</name>
        <name evidence="18" type="ORF">PGTUg99_016451</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="18 20">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPP phosphatase family. PP-2B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="13"/>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000110">
        <property type="protein sequence ID" value="KAA1130322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NF45"/>
    <dbReference type="OrthoDB" id="5593063at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07416">
        <property type="entry name" value="MPP_PP2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041751">
        <property type="entry name" value="MPP_PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="15">
        <location>
            <begin position="189"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="421"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="537"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="421"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1066038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1087152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1130322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="696" mass="76577" checksum="898493F311ED0190" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGK3</accession>
    <name>A0A5B0NGK3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-terminal acetyltransferase A complex catalytic subunit ard1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ARD1_4</name>
        <name evidence="3" type="ORF">PGT21_036242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGK3"/>
    <dbReference type="OrthoDB" id="5363962at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="149" mass="16724" checksum="C65F8B4F56DD5085" modified="2019-11-13" version="1">MKMDIARKIQAADEYIDLDRADAANQWAQLKKDNPHGFDVVVEATGVESIVNDSINYVRRGGTLLVYGVYDNAARVSWSPTKIFSDEINIIGSFSQTNCFPRAVAYLDSGRIRTEGMVTDIFKIEEYQQALDKMASRQCLKIAVRPGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHH4</accession>
    <name>A0A5B0NHH4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein kinase RIO1</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">RIO1_2</name>
        <name evidence="23" type="ORF">PGT21_031414</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHH4"/>
    <dbReference type="OrthoDB" id="205248at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05147">
        <property type="entry name" value="RIO1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001125">
        <property type="entry name" value="Serine/threonine-protein kinase RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000403">
        <property type="entry name" value="Serine/threonine-protein kinase RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017407">
        <property type="entry name" value="Ser/Thr_kinase_Rio1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038147">
        <property type="entry name" value="Ser/Thr_PK_RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="22">
        <location>
            <begin position="185"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="536"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="547"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="593"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="619"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="18">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAA1087428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="641" mass="72632" checksum="FCEDF1CD6E63CCAF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLE2</accession>
    <name>A0A5B0NLE2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">U2 snRNP-associated SURP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_024290</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLE2"/>
    <dbReference type="OrthoDB" id="377209at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF0">
        <property type="entry name" value="U2 SNRNP-ASSOCIATED SURP MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="197"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="6">
        <location>
            <begin position="426"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="CID" evidence="7">
        <location>
            <begin position="514"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="49"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="291"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="698"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1089593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="756" mass="84187" checksum="E26AA437D28929F9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NM00</accession>
    <name>A0A5B0NM00_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NM00"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022888787" evidence="1">
        <location>
            <begin position="25"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="566" mass="66184" checksum="FEEA75CEB6C6D625" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMD8</accession>
    <name>A0A5B0NMD8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">N-acetyltransferase O1 (Establishment of cohesion protein 1)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_001876</name>
        <name evidence="14" type="ORF">PGTUg99_003286</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. ECO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000149">
        <property type="protein sequence ID" value="KAA1127802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMD8"/>
    <dbReference type="OrthoDB" id="428854at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028005">
        <property type="entry name" value="AcTrfase_ESCO_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028009">
        <property type="entry name" value="ESCO_Acetyltransf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13880">
        <property type="entry name" value="Acetyltransf_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13878">
        <property type="entry name" value="zf-C2H2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="N-acetyltransferase ESCO zinc-finger" evidence="11">
        <location>
            <begin position="121"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase ESCO acetyl-transferase" evidence="12">
        <location>
            <begin position="326"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="56"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1090461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1127802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="44980" checksum="BE238B073AD07209" modified="2019-11-13" version="1">MVVPPARPRGHSTSTSESADRVLTSPLVKTTVRVVVPKLDDDSKRQRSTDIRSFFASATAPHAHQKNTPISSSPATTVLRTSPLTSPSSSRTLPTRKRTRLSDKKTINKQSSKTRRPMMSQLMLDLSNRPTTQICKECGMSFVLGVEEDVELHKKHHRRVVEGVEWPMTLLESQPDCRVVWREPGGMEEEKIVKVSLDPQSLKRSRYKHKVEQILEMVNRSLDAQELTEDQLRASSLYIYLGSPMDRSTGLPEPKKRRLSSGLSTTPKSKKKKVVVKAMCVAARIEHGYQIVRGPGNPGEEELDDAELLRFGETSSELYCSPKAEKTLIGIHRIWSSPHHRRAGLSRRLLDVVAGTFIYGMAIDGIEARRRSIAFSQPTESGMRLAADWFQTVLFKIFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSE4</accession>
    <name>A0A5B0NSE4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Enterotoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015593</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NSE4"/>
    <dbReference type="OrthoDB" id="2506484at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023122426" description="Enterotoxin" evidence="2">
        <location>
            <begin position="18"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="46256" checksum="2683244DFA634764" modified="2019-11-13" version="1">MLTVSFMALFLLRATLAGPIEFIPPSVPAPWGGAINLISASFNFLHEPQDRSPLSASPFLHEPQDRSPLSASPSPMASPSKSSAFSGPTSKKLSHPMYVQGPFDIIEYGEHRILDGQNYGQFNYTTSLIWNHPNGKQHAIAIDLSGYSSRAALFDKDMVYCLAGRFVGGIQQPADEAREPAVFFESAFDVCMGSSSAYIDKNERSLLADKVAVTGYGVVVDRQSIPGDNPNADLRVVLRHTDYHSVLKKKVEFDTVYYVPGNKMLSGIQKSIELNREWHSHITNRYPRTFQHSGNVTNFGPQWALDSRRPHLMRIGGFVQTHNSSQKSTASIASSSTSGDCEVHPFSASNVFPLPSPSSSKFAQDNPDHVEDGEVTENDKITFPGKRTAAIDAIGQPPESNQRPKRSASTLSDAQKKMKGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVE7</accession>
    <name>A0A5B0NVE7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVE7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1093135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="122" mass="14230" checksum="F53B5E590EAD3132" modified="2019-11-13" version="1">MFVYQEVDLIYDWLADESPRSETEGRSLKQKEDYLWKSHARLFAAWALKSSTVIVKRESQQYRIRIFRRTRQAFMEECNGPKGGDDAAGSFPQRFPVINPVLVVTGGGGKRDRLQRQLTFWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVU7</accession>
    <name>A0A5B0NVU7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MRM2_2</name>
        <name evidence="9" type="ORF">PGT21_018277</name>
        <name evidence="10" type="ORF">PGTUg99_029748</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVU7"/>
    <dbReference type="OrthoDB" id="20105at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000959">
        <property type="entry name" value="RNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="8">
        <location>
            <begin position="64"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1092932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1115740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="331" mass="36990" checksum="F118B4A7670E0F4C" modified="2019-11-13" version="1">MAKKLTTFGSDSLRAMSRTQGSCRRFSIVRLGGEQGGQSKQFLRRAETDPYGKTKSRARGEPTYVARSAHKLIQLDQQFRIFPTPARSSPRTAFRVLDLGAAPGGWSEVVLERLAHLHSHPAISSSPSPSAPSETPPSNRNLLPRSSVNHQHRLVACDLLPLHPSIAPKVSKNVDFHAIQGDFMDAQVRAKIVELLFDSTAVAHDHDNQGLVQEEKQEKETSDRRRTTTIILSDMLHSMTGIPTRDSQNSLDLSVTVADLARDLIPPNPGGSRASDTLILKHLQSEFTHQFRDRLLAEWSLVKWVKPLASRSESREGYFVTRGRRKQWIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NX98</accession>
    <name>A0A5B0NX98_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004401</name>
        <name evidence="2" type="ORF">PGTUg99_036148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NX98"/>
    <dbReference type="OrthoDB" id="2509601at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48209">
        <property type="entry name" value="AGL056WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48209:SF2">
        <property type="entry name" value="FI24008P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="789"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="803"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="846" mass="94436" checksum="D75B97739D598B90" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NY91</accession>
    <name>A0A5B0NY91_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014907</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NY91"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="239" mass="26883" checksum="00438F99973D8176" modified="2019-11-13" version="1">MPSQTRKNEFSLSYKYDVGNARWEGSILTPMGSGTVLRIGGLRIGDARLESDGLINIDMRVGTGQFSLNENEEEENLRGLPYSTLNKPYELRAPVGTESQPYYPSLYLHNLDLMTCGWTIPPAAQPMRLRTMDENVVGVVSRRGSRISVHQSSYRPEAPGPSVPLATMRQGVPTALSSYLDIPGGFQEKEIVVWRHRYSPFYAWDIEEHNSEVERTFSHMEGEGGPYDFGVGKRTCRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYV7</accession>
    <name>A0A5B0NYV7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NYV7"/>
    <dbReference type="OrthoDB" id="2564822at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22744">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34863">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34863:SF1">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="851"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="753"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1014"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="758"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1021"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1052"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1062" mass="120692" checksum="2B574EC6D56EF3FE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2N4</accession>
    <name>A0A5B0P2N4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2N4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022738426" description="Secreted protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14181" checksum="B6AEF1998E9DF4AC" modified="2019-11-13" version="1">MNVNISMIMIGLGIVLMAASLVASTQPQHGKVFNPSSYDFSSQVTYEVNKHLKCGHCENFYTQRGCKILDYTGPRPASTEQYCNIYYIKSFHSPTSYYCDNQYGEFVCYGGMKNPPGMICTQCHES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4N0</accession>
    <name>A0A5B0P4N0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclopropane-fatty-acyl-phospholipid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_018799</name>
        <name evidence="7" type="ORF">PGT21_019307</name>
        <name evidence="8" type="ORF">PGTUg99_033902</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFA/CMAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4N0"/>
    <dbReference type="OrthoDB" id="8300214at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050723">
        <property type="entry name" value="CFA/CMAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003333">
        <property type="entry name" value="CMAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43667">
        <property type="entry name" value="CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43667:SF2">
        <property type="entry name" value="FATTY ACID C-METHYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003085">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1096507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1113042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1131928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="55335" checksum="C9CBB2B195B646A1" modified="2019-11-13" version="1">MDEDYLKTKNYSQSSAILSLTNRSLHFLKESIVNTSTFSPIISLAKTTIISLMSKIEYGQIKLNTETESLVFGRSKVHPETSEELAAVIQVVNDAFWLRMFLLSDIGFAEAYMVGDIQVDRLDSLFKIFILNRSNLSEMSMITSSLFSTLNSIMNSRFVNSISNTISNISAHYDISNEMFQAFLSKDMTYSCAYFPPSLGGPDGDLKNQEEGENGISATGKDRLELAQYEKLDLIIEKAKIRKGDRVLEIGSGWGSFAIRAVERTGCQVDTITLSIEQKKLAEKRIRQAGLEQMITVHLVDYRDLPSRFKAPFDRIVSIEMIESVGVEFLDTYFDVVQRCLHPKTGIAVFQVITIPESRFDRYVKEVDFIRKWIFPGGILPSVTFMTNAISKGSKNRLLIDSIQNIGPHYARTLREWESRFKLNFDQLVAPCLVKTYPELVDNPHRIQIFKRKWIYYFEYCSTGFESRVLGDHIFTLTREGNLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAW0</accession>
    <name>A0A5B0PAW0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sla2 Src like adaptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SLA2_3</name>
        <name evidence="9" type="ORF">PGT21_029523</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLA2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAW0"/>
    <dbReference type="OrthoDB" id="10262320at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17007">
        <property type="entry name" value="ANTH_N_Sla2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1410.10:FF:000004">
        <property type="entry name" value="Cytoskeleton assembly control protein Sla2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000021">
        <property type="entry name" value="Cytoskeleton assembly control protein Sla2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035964">
        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030224">
        <property type="entry name" value="Sla2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407:SF15">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="7">
        <location>
            <begin position="46"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="8">
        <location>
            <begin position="840"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="302"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="498"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1098156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1086" mass="123329" checksum="3645F76877B4F981" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCH8</accession>
    <name>A0A5B0PCH8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription elongation factor SPT6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Chromatin Elongation factor SPT6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Transcription elongation factor spt6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SPT6_4</name>
        <name evidence="15" type="ORF">PGT21_002284</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Histone H3-H4 chaperone that plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression. Essential for viability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPT6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="995477at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09918">
        <property type="entry name" value="SH2_Nterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2740:FF:000002">
        <property type="entry name" value="Transcription elongation factor Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.850:FF:000001">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.140:FF:000007">
        <property type="entry name" value="Transcription elongation factor SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000056">
        <property type="entry name" value="Transcription elongation factor Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Spt6, Death-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH2_dom_sf"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR032706">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
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    </dbReference>
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        <property type="entry name" value="Spt6_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028231">
        <property type="entry name" value="Spt6_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14635">
        <property type="entry name" value="HHH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14641">
        <property type="entry name" value="HTH_44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21710">
        <property type="entry name" value="Spt6_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="15" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
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        <location>
            <begin position="699"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="978"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="1044"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
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        <source>
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        </source>
    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00020248"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1099287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1141" mass="128179" checksum="A777D993E1D2ADD2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PD71</accession>
    <name>A0A5B0PD71_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PD71"/>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="17668" checksum="84225BE0071045A0" modified="2019-11-13" version="1">MALSKNTRCYIFKKPGHIAPDCPAKKRGVNQSNPPVRTNQNPGPYPPCSVTYNFDQLPYIRPLQPESKAPNTASTKKVPAEHPSRAVEAKLINPDIFAEEEEENHFVFETEDLSAEPSGNRFNLRNLSINEEGQEVIWDSGASDNVTGDRDEQNNCRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFC5</accession>
    <name>A0A5B0PFC5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020389</name>
        <name evidence="2" type="ORF">PGTUg99_031736</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFC5"/>
    <dbReference type="OrthoDB" id="29523at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="29660" checksum="655A11ADDB7FEA34" modified="2019-11-13" version="1">MGLSGRKDKQKIGDDPRNTKWAKNESNPGFKILSSMGWTPSTTTLGAASTASEPAQSTWKRLPGVILPVIKSSTSGLGANPAQASTSTYLSGAPRFVRASQGISVVESDLEPSTLAENDPDRSTKLAIVSQGGGFDDLLSRLNQSKSKSKSKSKSKSTSKSTSITKSESETETSTTTKIKRKKSKKNSKSSEAEPTEEKQPTRKIKKSKKRKTMEATEPEQPTDDDEELVATPSGPIRISRPINPRVAARAKFINSKKLASSAGSAQAMAEILGIPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PG63</accession>
    <name>A0A5B0PG63_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PG63"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="11850" checksum="C9BBC90A56A6123A" modified="2019-11-13" version="1">MTEADSTCDGRYVIAYLEVNSGNIFRIALLYSFEFRIALLPRAIYKNHKPIAQVWFLSATIPYLFLLASRVSPENYRHFSSPSFPLTPYFFFRRFMILFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLR4</accession>
    <name>A0A5B0PLR4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024858</name>
        <name evidence="4" type="ORF">PGTUg99_029704</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000035">
        <property type="protein sequence ID" value="KAA1136388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLR4"/>
    <dbReference type="OrthoDB" id="10615786at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137788" description="HEAT repeat domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1136388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="47977" checksum="28487F7850A50DF1" modified="2019-11-13" version="1">MPLAHFISVFLSIGQLLILNTAASLTKLAEESNLAANAHLPTSAMEDASKLTTARGMTPAPQRDRLGNTPPWEFRAGELMKTTTLTEQSRDAALGRAPSYWHPYAIPLENRFIKQAQKTQEQKASAPGRNRGQKQSQRGQKSVFKWITNLFRKNRRGNLKDQTAPGAFGETAGSADKGTASPMVQLFNDLFQKDKKPEAHFKLAQEAVEYLLRTPGVRQEELSEWVPKLIQVGSKNGHGHNLEFRSTLFYGLHTLVLKHYANDVLRSSILSQLNKALERIRTEFGPAIVSEWDKELYNPSSGLSKKIGNWKQLKDIITYEPSLKDLRIGLNNKNKASIIQNLDHIKTIFAISTIRNYSAETRVASIGVLSEIIKQENSEHIKGHAALELALSLKDPENTEVFLFDHEKKLLESLTAQSNLREPANLPKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMJ3</accession>
    <name>A0A5B0PMJ3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PMJ3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="54" mass="6180" checksum="027898EC513CBB21" modified="2019-11-13" version="1">MTICAHDLLRSWTAKRGLKTVSEQHYCCPSNIPTKPPPRLGFAIRRYGCRANTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSG0</accession>
    <name>A0A5B0PSG0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029954</name>
        <name evidence="2" type="ORF">PGTUg99_014198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000414">
        <property type="protein sequence ID" value="KAA1086073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSG0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366446" evidence="1">
        <location>
            <begin position="23"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="100" mass="11393" checksum="0BDEB137E9994F63" modified="2019-11-13" version="1">MNRFFTIAAVLVTVTIIASIDSCTVKYAVASRCLTDGCKTKGWEDGRLMTGMARWECYNDHCPSNVIYKNKWVRVRDWCQGCVNARVRAGQSIKEDTNMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSM3</accession>
    <name>A0A5B0PSM3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSM3"/>
    <dbReference type="OrthoDB" id="2498246at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="14498" checksum="CF2900F5EC092787" modified="2019-11-13" version="1" fragment="single">MALDIFHNLVRLNGLDHKILRTTQPNHKRLFEWVYDLLFADTPDHLPLLGKSQVKLPLKISDKAFNGAQRFLHVYLTKPKRIGEFAASIAALDLLELWYKSQLSELDKSTFSSKEYKKSLFRAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTE6</accession>
    <name>A0A5B0PTE6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="11">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_050350</name>
        <name evidence="14" type="ORF">PGTUg99_050088</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="14 17">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9 11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="11"/>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTE6"/>
    <dbReference type="OrthoDB" id="361797at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9 11" id="KW-0274">FAD</keyword>
    <keyword evidence="2 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9 11" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="13">
        <location>
            <begin position="44"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="12">
        <location>
            <begin position="392"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="223"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="364"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="10">
        <location>
            <begin position="82"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1080173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1103900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="511" mass="54562" checksum="2A19971674288B9D" modified="2019-11-13" version="1">MISQAISRSYRLTPTLRPSTTFSSRSLTPQLARRFASQTDQKEYDVVVIGGGPGGYVAAIKAAQLGLKTACVEKRGALGGTCLNVGCIPSKAMLNNSHIYHQTQHDLKNRGIDVGDVKLNLPNMLKAKNKAVSSLTKGIEGLFKKNKVDYIKGHGALSSTTSVTVDLIDGGKQDLSTKNIIIATGSEVSPFPGGAIEIDEKRIVSSTGALDLQEVPKKMVVIGGGIIGLEMGSVWSRLGAEVTVVEYLGTIGGQGIDGEIAQSFQKILSKQGIKFKLNTKVTGLEKIDERNHHVKIESADGAKTDQLEADVVLVAVGRRPNTSGLNLEAVGVEVDQRGKVVIDSQYSTSVPNIKCIGDVTYGQMLAHKAEEEGIAIAEMLKTGHGHVNYDVIPSVVYTHPEVAWVGKTEEDLKKANVKYSIGKFPFLANSRAKTNDDSEGMVKFLTEKETDKILGVHIIGPNAGEMIGEAVLAMEYSASAEDVARTSHAHPTLSEAFKEAAMAATGKAIHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUB4</accession>
    <name>A0A5B0PUB4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone-lysine N-methyltransferase, H3 lysine-36 specific</fullName>
            <ecNumber evidence="4">2.1.1.359</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">SET domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SET2_1</name>
        <name evidence="21" type="ORF">PGT21_018546</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Histone methyltransferase that trimethylates histone H3 'Lys-36' forming H3K36me3. Involved in transcription elongation as well as in transcription repression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60324"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:15536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.359"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.359" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUB4"/>
    <dbReference type="OrthoDB" id="422362at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140955">
        <property type="term" value="F:histone H3K36 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19172">
        <property type="entry name" value="SET_SETD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        </location>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1104302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="983" mass="111425" checksum="DCA87040FE765335" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUE5</accession>
    <name>A0A5B0PUE5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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            <end position="61"/>
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    </feature>
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        <location>
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    </feature>
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            <begin position="14"/>
            <end position="26"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="476" mass="53629" checksum="BD0B1EC6935B5B30" modified="2019-11-13" version="1">MEAAPKITRSKATVRKDPETNIREAQETATQKGATLTPRESNPAQESGKQPNELEESDGEDRDYFLVLSADNLKEAIEITSIPPETKEVDQRSHIWDQLKLAQANGDKILAKILLAAYNELEPNTATSPKLTRSTSALPILSNAEFTKGVELSEAKTELEDNLVYAVGAVTSHQDIGFTPYFDENIKKLRAPLPLTIFDRAYRGLPYHDEWTQSHSTWTNNHRSFYITLRDVYNKGLFAEKLRIHKENCDSIADVYGFMTAFRYDMQIRMNAFAHRVPTKDGAAVPDISVKQMVVVEQCYSTVRSFGKASWKDNNYAPGFSHASFDPDTGVKKPELVKAASYQSGYNWESNGGTHRMNHQERRREKRWGGNSQEEHNRNSFNNNFPGNYGNQGYGPYTQNFGFKNQFGNRTSYESQFNQNQNHFNPYGQNYASHSQGPSDSRKRFRGGSGQVDDADGSGKSTGVKDGQVESGNRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PY99</accession>
    <name>A0A5B0PY99_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022928</name>
        <name evidence="3" type="ORF">PGTUg99_029085</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1105876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1121051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="208" mass="23662" checksum="078890AB822D73D8" modified="2019-11-13" version="1">MIVAGSQANTRSFWSQRHYGPNRPKAKGHMLTVYQNAKSNRNLAVSKSFCTSRQVSSNPKSGQVSCALVQEFLTWSTTPCQRPTYLPFAATRAERRNMARQPARQKADDRQSRPTWQGPFPAHLPMPEDICPHLPHEHLTEKEFNLTRKFHVSETRSGLWTLDSGQVLCPINSGLHQKMKLMTLAGLEPTTTCEHRDRWIMLSMCSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1X6</accession>
    <name>A0A5B0Q1X6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Altered inheritance of mitochondria protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">AIM41</name>
        <name evidence="2" type="ORF">PGT21_003269</name>
        <name evidence="3" type="ORF">PGTUg99_005404</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000010">
        <property type="protein sequence ID" value="KAA1137219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1X6"/>
    <dbReference type="OrthoDB" id="538640at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016884">
        <property type="term" value="F:carbon-nitrogen ligase activity, with glutamine as amido-N-donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.10">
        <property type="entry name" value="Uncharacterised protein YqeY/AIM41 PF09424, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019004">
        <property type="entry name" value="YqeY/Aim41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042184">
        <property type="entry name" value="YqeY/Aim41_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 41, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 41, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09424">
        <property type="entry name" value="YqeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="258" mass="28902" checksum="E3C2ABCAFBB3E30F" modified="2019-11-13" version="1">MVAGVLHPSNLLLHQIRFQSPHTAEDWHSRRFLRKTEEMGLARNGIGLRNVISRHLARESNVSPLPYLPRPWASKSSVSYFSSSNSTHSDSVEKGPDDLIASLRADLKNSMKIKDLTQRDVIKSLLGDLQNSEHNKNRQTHEKVVNSAIKKRLEAAKISLESNPPRLELNKQNLQEVEILKKYLTFFSSSQSAQDSNQEALTKAVNDSIQQLGLSPDATQQERQNSLGKLIKSVKDKVDSNFDSKDIANAVRKALGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q275</accession>
    <name>A0A5B0Q275_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001757</name>
        <name evidence="3" type="ORF">PGTUg99_008879</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000147">
        <property type="protein sequence ID" value="KAA1127909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q275"/>
    <dbReference type="OrthoDB" id="2502269at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137887" evidence="1">
        <location>
            <begin position="20"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1127909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="174" mass="18970" checksum="2D8BB699F9334BCD" modified="2019-11-13" version="1">MKFLAVLICFVALLSSRSAMPTLKTKLLTFHTNGKPVNLPSDSILADPILLTTKGAQTQVVEIDGKQVLRALLIRDVPNDARGFLYIEGLAQDGEVSYAVWLPSVAGYFMRPRGLQSGTIHRFIFDNLKSHQSPVILVNGIVDVGQSTTGSTPSEEERLSTLLQLDTFSESAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2E9</accession>
    <name>A0A5B0Q2E9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2E9"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25499">
        <property type="entry name" value="Beta-prop_pof12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="511" mass="56874" checksum="4745F96C0CC50F34" modified="2019-11-13" version="1">MESRASRSQLDRKGKRKHLEVTIDQESHLGHTWKTLCRISWNWKRGSAGLTRITPIPNQSDSPPISTSPQARTTSTRSQGSKNPTPNTIVRFHRSLLFIARKSRSSESLPTVYVYNLQNDFNHQQQKKSVLIGKLSPALGSQDVSPFVGQAVTEISIDESCGTNSETTQAVKSTVLLSVFYTTGQFTLYEISLPDVSPDTGDPIVLDFQEVGSFQPYLTSKISNSRKVHHGLVETAKFHFPLLVTCSVDFHLSFFRLSWSSSPAQPTEKLQIIKLALDMQNHSCYWPLSLSLKRSEPSSERFKMTIAYPMPFYPSAWTVGMQEFDFEITSSRSSSASSSTTSLQMNTNRFITAFQHPQDKDRRPMAGLSLGEIVIGIEQAAENVIVGRSDNTIDCFRVASVSDDDASDHRMKRLLCSKTLFGHTSRIGSITVDENGRCVSGAMDGVKVWEGSEAVDVRSVDSSASSLLETRSVGWIGSDSEKIVSLWIWEDLDPNANTARLNEEIRVLSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q523</accession>
    <name>A0A5B0Q523_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q523"/>
    <dbReference type="OrthoDB" id="10317913at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="13846" checksum="DEF32778827FDF0F" modified="2019-11-13" version="1">MRQTADDDPEVTRRSLLILKEINAVPIQIYRLRVEIVEGAQVNPVALSAGQPHGQSMAPLNEKQAKHEGLVETLERVVGNPIAGELSRPACNRRLFGTAGDPVGRREGSSQHAHVGLLGSVTPESGRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5I2</accession>
    <name>A0A5B0Q5I2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5I2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="166" mass="17859" checksum="C2557DFA4D27F2F7" modified="2019-11-13" version="1">MFKSKALMLRVCAGTAITLSTLSFQLVVLEARHTSLVPRDGFVKNCVRFVGEYYDDKSKLVCEDNDTGYLCPKDQCTSGDLPGSHAAAPFSTMTWSGCKRLSSGGIPEGEGVTASQAITVQFLPGQQVAVHGYQSGLSGIFDFECHWSSPSDPNARTPYCGGCQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q604</accession>
    <name>A0A5B0Q604_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Histidine biosynthesis trifunctional protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="19">Phosphoribosyl-AMP cyclohydrolase</fullName>
                <ecNumber evidence="19">3.5.4.19</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">Phosphoribosyl-ATP pyrophosphohydrolase</fullName>
                <ecNumber evidence="19">3.6.1.31</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">Histidinol dehydrogenase</fullName>
                <shortName evidence="19">HDH</shortName>
                <ecNumber evidence="19">1.1.1.23</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="22" type="primary">HIS4_2</name>
        <name evidence="22" type="ORF">PGT21_019296</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 19">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>L-histidinol + 2 NAD(+) + H2O = L-histidine + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 19">In the C-terminal section; belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="19"/>
    <dbReference type="EC" id="3.6.1.31" evidence="19"/>
    <dbReference type="EC" id="1.1.1.23" evidence="19"/>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q604"/>
    <dbReference type="OrthoDB" id="1703565at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11546">
        <property type="entry name" value="NTP-PPase_His4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1080:FF:000002">
        <property type="entry name" value="Histidine biosynthesis bifunctional protein HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000050">
        <property type="entry name" value="Histidine biosynthesis trifunctional protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000001">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1300:FF:000002">
        <property type="entry name" value="Histidinol dehydrogenase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="entry name" value="Phosphoribosyl-AMP cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016298">
        <property type="entry name" value="Histidine_synth_trifunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001257">
        <property type="entry name" value="His_trifunctional"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="HisI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 19" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16 19" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="11 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15 19" id="KW-0520">NAD</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphoribosyl-AMP cyclohydrolase" evidence="21">
        <location>
            <begin position="248"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="109"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="421"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="421"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR001257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAA1108611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="888" mass="97647" checksum="ACCCCB0828965B42" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAR7</accession>
    <name>A0A5B0QAR7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_013769</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAR7"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051480">
        <property type="entry name" value="Endocytic_GEF_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006:SF7">
        <property type="entry name" value="DH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="137"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="223"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="710"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="847"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="712"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="847"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="864"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="888"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="922"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1110192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1023" mass="112152" checksum="0C0CCF1959F3FB62" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAU5</accession>
    <name>A0A5B0QAU5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAU5"/>
    <dbReference type="OrthoDB" id="10487308at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="119" mass="12449" checksum="1979E8B18AF7E731" modified="2019-11-13" version="1">MSLESTRNSNRPGTRSGTAGSSAVSYLGFARSSAGLLARFSRFFSNLTSSTSVGTQSQPNHHLTRLPAAASAPASSNGIASFIGINGRLLIHWHRLLAGLLARSPRLLSNLASSIGDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB13</accession>
    <name>A0A5B0QB13_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_020944</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2117453at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="6">
        <location>
            <begin position="24"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1110418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="189" mass="21082" checksum="EBCD3BAE9046E57C" modified="2019-11-13" version="1">MSTATPAHHQSLLHRLILPVIYSFVFLLSVIQFIITAVLVHDYEDHGYPNHSIRDRLRFLLFTSCWCLLFIPIYLFANLRLPGQALSSVLSNVGFISLTWLFWLCGAASWTDALGGTLHCGDLFLRNQVIDVSIPYCGSLRAVQAFAWMIWLLFSAALLYISTVAFQAKSHPTGLKGPMREVPAPTSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB57</accession>
    <name>A0A5B0QB57_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013884</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QB57"/>
    <dbReference type="OrthoDB" id="2507309at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="28590" checksum="D6A53BE78F1EDEBE" modified="2019-11-13" version="1">MSAGKPILPPAGRLSNQSALVAEVSEHNFFPDLDVASVLVFAAIDEELCATAEDRLVPSNVPRRLIICFKFGDRYLLGLIDTGAEINLVNDQTVKDIGLPMLNLVNPTRVTLALNDKSRTPLILKHFTRVTLADTRSGLSFDNVELSLGPVAGNYDMILGIPFLSHFHLSVSISQHSLVPPSEVAAATSSEGPCVKFDASSGFLAAQRHKKPLLKQLPPRRLKQTEHEHRGQTWTLSPVIGSRTQVTRTSDGRNQIDQNLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB88</accession>
    <name>A0A5B0QB88_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QB88"/>
    <dbReference type="OrthoDB" id="10634661at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="275" mass="30035" checksum="9A2FFFC9DC8EF05A" modified="2019-11-13" version="1">MGSQDGTYLSHNQNKENTNAFTTENGTSGSKSPARANSPKYDEPNAASTAPDTIQVILTIQLGQIRFGNANQDQSLPPNPPTNLNGNILTASSGVEDGPMVNLSPRQSAILSILGAKACDKSAPSNDLHLPSLGNAPSHLVVGNLKDTRLDRLKELDRIAIKINDSCCFGAPPHPQFLHYQFAETNNRALNAKQTSTPSTSWTFEKLNHPPNSYLKRILLNGRAVLPSNHLPTPSSQRDTCLNQREPKMGNNEMSNRIDPADDDVDHHLNFEQWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCB6</accession>
    <name>A0A5B0QCB6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCB6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8213" checksum="8F9C00FBA551B8B1" modified="2019-11-13" version="1">MVSSGTTSISSILLNVVQHSSQDTSSVVINIKVYSTPVTTTTTTTTTTTTTTTTTTTTTTTTTTTTTHFTLFIQNHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIT7</accession>
    <name>A0A5B0QIT7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIT7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="65" mass="7035" checksum="C467BDD2633CA151" modified="2019-11-13" version="1">MATGTLQAARPRGIEGPHPLDVELITGFTFMTTRSNTDPNELLPLTDPEAIIRSGNAEQRPSETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ21</accession>
    <name>A0A5B0QJ21_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ21"/>
    <dbReference type="OrthoDB" id="4743193at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="106" mass="11554" checksum="836C84AFB578AEBB" modified="2019-11-13" version="1">MKGASGLQDIYQSHKSAITHLSLTTFLQMARDYDIASAGAPKRRQKAPETTPDIFSKGLKFIADDFSQGGSKINRFSPATALLYEEFLSVEEGAVETEENIVNAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNZ8</accession>
    <name>A0A5B0QNZ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNZ8"/>
    <dbReference type="OrthoDB" id="270318at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="415" mass="47600" checksum="5561D1BCB9B55E72" modified="2019-11-13" version="1">MTDRRIGLTTTRLLIGAIRRTGSTNQQQRQRRRGYSGLSSIEECLDSLKRNDPESVLQLPFWPKESQPGFVAIKSFHGEINNIPNAVSRSQKPLIAQMRYQWWRDAIDSCYLSSSSSSQTVDNSPINQIPSNHPLIHLLKPIIKQHKLSKYYFARIINASETHYLDRRLPTLTALADHSRSTTYASLSLLAQLLTSSKQWKEKGTKLGGVTLATVDHSLSHLALFLTVVRTLKQLPYDIRHRGTHSVPLELLECSEETLFRFFNPRRTYNNHQSLASSDQKDLHDAQDSLFNLIFLAWSELVAAREVISLDLSHVHSDDFRNHQNATLIQKHILSSRKSYLPKITIDRTLIPLFLPATPARTQLTKISNIILNTHKARRQEEQGNLKSHLIDNVARKADWTVPFKLWFNFTFNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP35</accession>
    <name>A0A5B0QP35_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGT21_019144</name>
        <name evidence="17" type="ORF">PGTUg99_028600</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
            <strain evidence="17 20">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="VDEP01000338">
        <property type="protein sequence ID" value="KAA1102342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP35"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Photoperiod-independent early flowering 1"/>
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        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_CTER"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
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        <location>
            <begin position="235"/>
            <end position="399"/>
        </location>
    </feature>
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        <location>
            <begin position="537"/>
            <end position="688"/>
        </location>
    </feature>
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        <location>
            <begin position="894"/>
            <end position="946"/>
        </location>
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            <begin position="1"/>
            <end position="114"/>
        </location>
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        </location>
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        <location>
            <begin position="848"/>
            <end position="867"/>
        </location>
    </feature>
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        <location>
            <begin position="1081"/>
            <end position="1162"/>
        </location>
    </feature>
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        <location>
            <begin position="951"/>
            <end position="978"/>
        </location>
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            <begin position="1"/>
            <end position="19"/>
        </location>
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        </location>
    </feature>
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            <begin position="67"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
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        <location>
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            <end position="1150"/>
        </location>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
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        </source>
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        </source>
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        <source>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1102342.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1114705.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1162" mass="132514" checksum="47FB02DDC7D339F4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ51</accession>
    <name>A0A5B0QQ51_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 71 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022839182" description="Glycoside hydrolase family 71 protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="54682" checksum="3D426A38D83A5D8C" modified="2019-11-13" version="1">MLAAKWPLPFLHILLSNAVFQSLGDKQHFQLSTRAPPAAAPKLVFAHFIAGLTIDYTRDDWKNQIGLAASHGIDAFALNVGAPDNWQVDQVATAYDVASQITRTNGQPFQLFLSLDMSVIKTATEVSSWVTKFAPMKSQLLIGGKAFVSTFAGESNSFGANNASIGWQAAVKAPLAALSPPMDATFVPVWSSLNPSTAVSTNPVVDGIMTWKAWPTGNETTSTSTDLQFQADAKKNGKLYMASVSPCFYTHYSDKNFMFKSDNNLYINRWEELIAMPIQPDFIQIVSWNDYGESHYIGPIAGSPPAGTSWISGFDHQAWLDMTDYFIKWYKTGSPPAITEDKVYYNYRPHAAAAVATADPFGPPANATVSKDAVYAAVFLTSDSTAQQLRITIGAASQIFNHLKPGSISTFSAPWSGNGGDVRVELLDGSGKPLLSGTGQRPISNAIQAYNFNYASEVLMKGGGGSGPGYNSVSSQSYAKSDSSNIEILPFSRMSALAGMVALSLCSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQU7</accession>
    <name>A0A5B0QQU7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dynamin GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036952</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF235">
        <property type="entry name" value="DYNAMIN-RELATED PROTEIN DNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin GTPase" evidence="2">
        <location>
            <begin position="31"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="730"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="789" mass="88653" checksum="B6A3821D121B3499" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QS83</accession>
    <name>A0A5B0QS83_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QS83"/>
    <dbReference type="OrthoDB" id="2497430at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="681"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="754" mass="85107" checksum="58E4DD6669F24CF6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSC9</accession>
    <name>A0A5B0QSC9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <end position="18"/>
        </location>
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            <begin position="19"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="322" mass="37062" checksum="7565B9CEC752B9B8" modified="2019-11-13" version="1">MSLTKCMVITTLIAGVWGVKVHQLAVDLKFSQHNDHYMIFDTEPHKSPNFVKEQVILKTQSPPQSFSWWFPLGDCIRADRNMIEKKSKNVVPLEEKRNDEICITNNLRIEKVKNYGRTPFSVYNGVPEEPIMNFEKDGESELRWIPEKRFLLATNHLGAVLNEELYDFLVYPFLAAVKEGSNSMPTEYLYAETWQPSFREFILRVSNIGRTNHKSEAKYTMFRVDHGVTTNKSFSVGPRGQNGEMYARSITNKTQGKLRVIQTGIHEMNPENVLVGPYGSTRVLFDPSFPWVVVGPRKPPINLPHQDMYILNVHHVQVFFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QV02</accession>
    <name>A0A5B0QV02_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_018647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="76" mass="8700" checksum="10D1099C0F3B3237" modified="2019-11-13" version="1">MNLFLADQKVLEKDLNLLTDDLAREYFELKRKEILARRHAKYPQFFSPPSSSIPQATSSAANESSSTSENRRIETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVV5</accession>
    <name>A0A5B0QVV5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1117442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="399" mass="44715" checksum="50ED8994F29AF630" modified="2019-11-13" version="1">MSRFGIDLFARKNPPPRMGYHTDAENYHIHGIGPQPANLTPQMVHCSTTFLLYCPKQDKPGWNTVKPENVFPIVFEIGSLSLKDFQSLVATEANKLVDDAGGLINNAVETGSPRINWKVSLALKSAPEFKKALQYTVNEEKSYDHWIKTMKDLNADHTHAGLYVHMVNPTSIAKKAKVAVDMKAHVLTQHAAQKAGSSSATGNQHGPPKATDFKAVNVIMDQIYALHPPNIKYLDRIPVFIHPTEHSQYIPLTGKNVQIWAHAIMQSVDGVTLHSPPPELKFEKLSAYKKRKLSHSTVHDASPPTYQSSSRTQESESDNSSVVEPDENLMAEYVDFVKIKPTKKEEVLRILNEKDVTNPKFFQSNSITREDMSHWGLTPGIIAQLRDNTKKFEKRPAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QW58</accession>
    <name>A0A5B0QW58_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QW58"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="579"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="640" mass="68135" checksum="A50C1B52B6C9AD06" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWT3</accession>
    <name>A0A5B0QWT3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004824</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWT3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022765405" evidence="2">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="10834" checksum="BC1D4503D5B1F3F6" modified="2019-11-13" version="1">MMHILFNVTLVTSLLVNLVLGSFGLDPKAVANAAPEESRQLAVVGGNHQHSSLLHLHPDSSNDALQKSDISPPQAPSRIVPLSNFSGMPSSITFQLSPVLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX50</accession>
    <name>A0A5B0QX50_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_026505</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QX50"/>
    <dbReference type="OrthoDB" id="5592486at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="3">
        <location>
            <begin position="64"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="363"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="421"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1117887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="453" mass="50549" checksum="00F5244F5D1535CE" modified="2019-11-13" version="1">MNRFAVRGPSSFPRFQIHYWGKLLEILRSRLGVKVIVGKVPPTGTIEERAVELDKLLQSQPNTSRRPPKYNFIAHSMGGLDARYLISHLNPQTYTPISLTTICTPHRGSPFMDWCRANIGVGLSATEASQKRVPFSLKEPLLRRPPEPLGYLPNNLLKVLLLNLLDSPAYSNLSTDYLTGEFNPRTPDRAGVEYFSIAAKIGKNGLSLIHPLWLPGLLIDRLVETHEQGNHDGLVTVQSAQWGRFLGTIVGTDHWEIRGSSAFGAEILPEQPDDDHLLNLHAKSNWLELNRYIGSWLSSSDNSTNSQKLSKHNTSSKNSDSHSSSDSSDAALHKIFDQQTLSTRLLADWIAKKLPLNLYPSQPTLATSSSSSSSSSFSTPHKNPSSSSLNTYSKYPFTNPTRTVHPQFPLDDLDLNHHQHNNNNNSNQKNSANGFDIEKFYLAVLRNLYDHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYJ0</accession>
    <name>A0A5B0QYJ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sphinganine kinase lcb4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LCB4_3</name>
        <name evidence="3" type="ORF">PGT21_032623</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYJ0"/>
    <dbReference type="OrthoDB" id="3853857at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001727">
        <property type="term" value="F:lipid kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055916">
        <property type="entry name" value="DUF7493"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF31">
        <property type="entry name" value="ACYLGLYCEROL KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24321">
        <property type="entry name" value="DUF7493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="2">
        <location>
            <begin position="158"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="570" mass="63367" checksum="A04932A8569DA048" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0S1</accession>
    <name>A0A5B0R0S1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Geranylgeranyl transferase type-2 subunit alpha</fullName>
            <ecNumber evidence="6">2.5.1.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Geranylgeranyl transferase type II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_009685</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to cysteines occuring in specific C-terminal amino acid sequences.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0S1"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF2">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1118893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="455" mass="52223" checksum="C63604BF5AB969C9" modified="2019-11-13" version="1">MEQPMSPEALCDRLNILLSSLPITEVEIFKTPSTITTPNPHHPFLVYGNHQLAIPQKTLAIIYASVSQRFHRIYENNPTTGQQDPEEVFQLTRLLLIQNPDHLTALSTRRKLIDHHLPRHPDQLAKELELTKLLLSIASHSKATALWFHRRWAFHHLFPPSTLTQPSTRSDEDKFSSKIYRPLADLPPQVLKEELEFSLKTCELYPRNYYGWFHRKWLFYQLVHSADRSSSMREEEIEAERVRILDFIRLHPRDHSAANYVTFLLSCAPLPSVPSVPHPNQQPEPLDSVSPFIRDCYAALAQYPHFESSWSFLKFLALSNTLSLTQSLTLIQSTPIVLNLRNAILAAPAIPAPSSPKPSRHPEHVLSSSKITLADLHADTLRHSQTLCLRTLYFLGSHFKWIQWTDEMALFLLKLTTHHHPSSSSSCFINSIFTSDSDSIHFLDSLKILINRFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1E6</accession>
    <name>A0A5B0R1E6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1E6"/>
    <dbReference type="OrthoDB" id="2509063at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022798598" evidence="1">
        <location>
            <begin position="21"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="239" mass="25600" checksum="2EAA0FB519B3E9A8" modified="2019-11-13" version="1">MLSYAHIATLLCSVMPLIEASAVRTVLPGTGQLTHGVSHISRGNPSLSTKAALLRVKSDVLARRDDEDPEGTSVSVSVHAPQVILKIWADLNLAADQCRSAFQQQAPLQVAIEVASSLSVQVNKVNNQASQCACNNDPAAADVSVQLKAIVPPLLLSLQAVIQIGPQKYADAWDAQLKPIFHDCSKVFISLKKISLELNIDLEAIVKQLHLDLNVFLSVGIDLNSLLGLHVELLGLISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1Y8</accession>
    <name>A0A5B0R1Y8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1Y8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022775556" evidence="1">
        <location>
            <begin position="23"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="232" mass="25793" checksum="20FF84759086A30F" modified="2019-11-13" version="1">MRISVVMLYFLILVMQYSRSMARPNLRPGEGQELHPFIGHAVGSEFFAGFPTEWDDRNSHISRGQVGSQLIGTGASYQRPEKRLKKNPEFCADDALLSSSHLAREEASTLDELGGGAHKLGDFPAPEATFTSHTSLLPIPSSRKISEGFPLSPFPPSTLSLAGVFETFEEEMLRPHPNLDLFYDYVQPSLLHSFPPLEGRPSDCHGLDYFHSIPILESGNSPHQIFIEKNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R275</accession>
    <name>A0A5B0R275_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CBS and PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_030427</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R275"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17781">
        <property type="entry name" value="CBS_pair_MUG70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17782">
        <property type="entry name" value="CBS_pair_MUG70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051462">
        <property type="entry name" value="CBS_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108:SF26">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN DDB_G0289609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="721"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="130"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="197"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="309"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="376"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="6">
        <location>
            <begin position="559"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="462"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="673"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="681"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1119627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="745" mass="80862" checksum="6DE48CBBC766E273" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2J7</accession>
    <name>A0A5B0R2J7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">signal-recognition-particle GTPase</fullName>
            <ecNumber evidence="11">3.6.5.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Signal recognition particle 54 kDa protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SRP54_2</name>
        <name evidence="15" type="ORF">PGT21_031179</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="11"/>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2J7"/>
    <dbReference type="OrthoDB" id="10250817at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.30:FF:000006">
        <property type="entry name" value="Signal recognition particle subunit SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="14">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1119660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="635" mass="67831" checksum="A133A7F5F11EC702" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3C6</accession>
    <name>A0A5B0R3C6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_037030</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3C6"/>
    <dbReference type="OrthoDB" id="77989at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="177"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="22"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1120036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="538" mass="58535" checksum="191F9F8B634882EC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3W0</accession>
    <name>A0A5B0R3W0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_037254</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3YL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3W0"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11525">
        <property type="entry name" value="SYLF_SH3YL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033643">
        <property type="entry name" value="SYLF_SH3YL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF2">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING YSC84-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="602"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="221"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="378"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="291"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1120197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="661" mass="71967" checksum="3F5808CADBCA50C1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4I0</accession>
    <name>A0A5B0R4I0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_037250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4I0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1120193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="171" mass="18862" checksum="FE4A1E8BBC61D1D3" modified="2019-11-13" version="1">MLAPSGVCMAKLPVLKPSVVVYLRRADLPFRPRPQTGGFQDTALLTSVPGTQCLTDPSSINLASFRRFSSPGQQGKTAIDRRTTHRINIKKFILDFFQNFGSTCFLVTLHGNPSPVDIHRGQIAQHPFDHREAFSFVTLISSKTTQNEAECAQLVKQHGHPSESVPTTGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4I2</accession>
    <name>A0A5B0R4I2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003129</name>
        <name evidence="3" type="ORF">PGTUg99_003287</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000244">
        <property type="protein sequence ID" value="KAA1120372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4I2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138049" evidence="1">
        <location>
            <begin position="21"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="124" mass="13884" checksum="1B1D9E99E6751DA0" modified="2019-11-13" version="1">MPHHVATLVFFAYLVSTVSTDEHLEIYRPIDIWNQAFNSSSSTLKIHVCCAVGLELCNIPPLRPVQYPSSPAWSSIVFTYHITVSDASDPDHQAHQYPSLKTEPSPYQALHQGNSSWHIQPNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S0F8</accession>
    <name>A0A5B0S0F8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HhH-GPD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_023564</name>
        <name evidence="7" type="ORF">PGTUg99_026053</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkylbase DNA glycosidase AlkA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S0F8"/>
    <dbReference type="OrthoDB" id="415889at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032131">
        <property type="term" value="F:alkylated DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043916">
        <property type="term" value="F:DNA-7-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000004">
        <property type="entry name" value="DNA-3-methyladenine glycosylase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051912">
        <property type="entry name" value="Alkylbase_DNA_Glycosylase/TA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003:SF5">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="5">
        <location>
            <begin position="128"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1096639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1131796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="352" mass="39413" checksum="7A0FA6F255061076" modified="2019-11-13" version="1">MTAVSRVTRSTTRKAIAEAQTKLSASSARLTSKQTAGPLEDEERRPRKKRSKARVGASSQDDDQSLKTASQDPTRPFVLSEARAHLVALDDRFSLLFGRLPCGPFDNLSKSSIDQVAEPFRNLCCSILGQQVSCLAARSITYKFIKLFQPELPPKLEPTTRLSEFQFPSPSDVLHTPVLTLRTAGLSQRKAEYIHDLAQRFVDRRLVPQALLTMEPKMVVNELCKVRGIGRWTAEMFLIFCVKHPDILPHADLAIQKGILRWYTTALLPLKAEDGEGSKKEAKEGDAGLSTPPIPVGCPLSRQELSRRLTKPLKPGLFLTPLEMEQLTEQWKPYRSLPVCYMWSLTGFIPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S299</accession>
    <name>A0A5B0S299_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_027542</name>
        <name evidence="5" type="ORF">PGTUg99_019685</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S299"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF211">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="642"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1081012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1131163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="844" mass="95729" checksum="3A3EE134969ECB5D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S3X6</accession>
    <name>A0A5B0S3X6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_008380</name>
        <name evidence="10" type="ORF">PGTUg99_011147</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S3X6"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044843">
        <property type="term" value="P:cell cycle G1/S phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20559">
        <property type="entry name" value="CYCLIN_ScCLN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000010">
        <property type="entry name" value="G1/S-specific cyclin Cln1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="108"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="8">
        <location>
            <begin position="203"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="344"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="353"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1080464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1131424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="459" mass="50779" checksum="32391C5FD773B434" modified="2019-11-13" version="1">MPSIYNSARPSNGTQAISKKRPSPTCDDRDAPMKKRSAPLAPSGNFVPISPKTRHQILEKEMQTLFELEYQEEVVEYMHEMDTQTRPVVETIDLQPELQWYMRPYLIDFLIEIHLQYRLRPETLYLAVNVIDRYVSKRVVFKKHYQLVGCAALWIAAKYEDAKERVPSVQELSTMCCGAYEESSFLQMEGHVLSTIGWQLGHPTAEAWLRLFSIGAHRQQAGDFEAASTGHLARFMMELTLFHRSYVGLKSADISGGALLLARYILGKPRRASDESEGVLAVARLLDSHLGEHMSAISAVVMKKYAHGHFSRASTIVKEWYGQGHRFGGPETSASLPAHMHHVAQPTPVSKLSSSSSTTSISSAASSSRHSQLTPGSLSSLSLNSSPTSSISSYPSMECHSNASPSSNSADDDSRESSLMCSPQLACPKKSARSDIWPACSSDMNQDNSVSSVQMSRNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SE35</accession>
    <name>A0A5B0SE35_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012906</name>
        <name evidence="3" type="ORF">PGTUg99_022701</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SE35"/>
    <dbReference type="OrthoDB" id="2503624at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="786"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="541"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="788"/>
            <end position="819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1136030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="903" mass="98650" checksum="68C018644263B660" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SIK3</accession>
    <name>A0A5B0SIK3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004754</name>
        <name evidence="2" type="ORF">PGTUg99_019910</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SIK3"/>
    <dbReference type="OrthoDB" id="2513962at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1137798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="414" mass="47879" checksum="FD35E5447111CAE2" modified="2019-11-13" version="1">MSLKNIIEWLILIDMAVLKDLKLVEPNLDQSSSNRELSNWLFAQAFKPQNSFPVFGMLETTHHLEPVKEFGRIQKILLEQLSRHDPEEDSLEASILIIQSWYREFHPQIWEHINNNQDGYSPEIYLLLALYKSIGSGSTGECDQEDEKFTDWDSLGSLKIPDLSRFPVTMKPERFLERHLSGDGSIEPSKDLLVFGSTDFVADSIQVEGLSVRIRRYTHRSEDTPSNFKYRIRVGDGSPTDGRLRTIVSKFNLLDTHLSICHREIIHQLKSQGKWVANDALKSFSQWVKDILLGTEGNSLAIIGDIEKEEDGEFQLDEFGEVQIYLVNQYLGHPMPYSRVIWVSLVLLEYWYRKFHSECFFPTSNDYWNSMIKSLGIKLDHAGRYSLVKYDDQGNFVGRVHRKKSLRSKRLASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LML7</accession>
    <name>A0A5B0LML7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LML7"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01846">
        <property type="entry name" value="fatty_acyltransferase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF139">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022941889" description="SGNH hydrolase-type esterase domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="40991" checksum="D482F36ABFCDEF71" modified="2019-11-13" version="1">MWISKHRTCNRFIRFVVSPLLVITKASCLVDSPSDPSELQESFQPSWTNYSAFIVFGDSYSDNGHPRSPEDQPSLAAKPAVGGRFCDGPVWDEYLAQNLSNSADSNITFLNYAYNGAHIRNKLSNQSPSPVPDTAAQIQTYLTDLEKYVQHPISGKVFEGRILHALWIGINPILAIWRSASSGNQTLNADTLLTPELAETLNTQVEEFSKQLLQLSNNPSLSKFQTDYMVMTIPPLTNTALALSQSSQASKGDVTLAQQYIELLAQMTDHFNTKLVEAVQKMTRQFSWHKRRITVFDTVDFWDDVRSAPQRFGIQSLGSCYTDPNKPPCKKPDLYMYWDILHPSSIFHANLSNAIMQFLDELNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZW9</accession>
    <name>A0A5B0LZW9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZW9"/>
    <dbReference type="OrthoDB" id="10442167at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="212" mass="22560" checksum="EC46DF3F4573216C" modified="2019-11-13" version="1">MPYPTSKSNPTSRASSTRASNPTLVPAGEFKRGKSKTPTPKLASGDNPPVRDARSGQESLSPRRGSEQSGSDGNPSKASLSVPPKPGRVDRQRYSPIFDGRLESVLSQPHKTSSTPTTKPTATIPQSSSSSTSERFERARSSPEESGQSSSETRKDGGTTKSSPRSVLGNDPNVQNPPRDLSTSGRSLQNQPRKELSPFTLRDAKKSLVGAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0U0</accession>
    <name>A0A5B0M0U0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0U0"/>
    <dbReference type="OrthoDB" id="5581181at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044554">
        <property type="entry name" value="ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057975">
        <property type="entry name" value="TPR_ANAPC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25773">
        <property type="entry name" value="TPR_ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="2">
        <location>
            <begin position="469"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="702" mass="80281" checksum="6FF332D0BAAAD5B9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1I9</accession>
    <name>A0A5B0M1I9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1I9"/>
    <dbReference type="OrthoDB" id="2246127at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="414" mass="47160" checksum="BC61B246D430AB7E" modified="2019-11-13" version="1">MTISGFVTVSQPDGRMKYVCQVCDGRGMDLVYLKRHLNTPGHQSNALRRAMEETRRTIEAQVEAQLNALQEDDIVNDAEELFIPRRLLDVHNEAYHPPQVDLNDQTGMGFGGADSSSNESSSGQPVCRGTMDWDQWNMPEDYSQFTNDYVILPGQNHVLGNSHDDEDMNPTDLWYPFPAKEYLISTLMLGHLHNLLSRNVYHLVRLSVSLGCLDIPHWDTIRRRREQIRKMLDVKLNENISVLDNKVFTLSLKDTISHELSNPYLNNELYAKCIAPKMKNFATTELPNNQPQIQLMIPQMISYDSPQLINIPVRMFDLMCSEMKWGDGTPFLTKIRRQLVEVSEFGGPDNTSNVAGALEIAEPIVNQINELATDDAADRTGIRYIQQFFGIPHLPKPRSWIDTKENAFLRKGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M402</accession>
    <name>A0A5B0M402_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014483</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M402"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="87" mass="9434" checksum="162222722AB1687F" modified="2019-11-13" version="1">MTIRPKSYSLVGQNRTQPPAGERLLLQILGSYQFVPVYGCLCLPGNSGIHVGLRISIAHLTVHLVVTHGLDIEQKNLSSKQSPAMSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M4D4</accession>
    <name>A0A5B0M4D4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018810</name>
        <name evidence="2" type="ORF">PGTUg99_016804</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000479">
        <property type="protein sequence ID" value="KAA1071206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M4D4"/>
    <dbReference type="OrthoDB" id="2947226at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="352" mass="40005" checksum="70D23408C1BA2B48" modified="2019-11-13" version="1">MSLPRVKILNLNNRHRTQFLLSPPENQPTPTRFVQITAQLIQIPLEGSPQSTIPSTNCLPPGTIGVPQHPPLHLNCDNRAAVLVLDNNASKGRMKSLERNFFFVNDAQTLGYNQSASLHNISYPFVKKLKVAQIDQISTPEDLKIAHPGYNIQQISELNLNEKHVLKKNYFILFNITHPQEVQHIGSINSIWKVQKPFHQSMYFIHTTLFQKANKNSYYRMREIWRTPHSTFVNSQNIEAGLNVQHNCNRGECKLLETRIATVEQQKSTRKTLELTHTNTDQYIVNLASLSSAPSHRKFSDIVVDSAGPLNWVDAMHDGSKKWGKNVEKKEKKAKNKASTSSQARMDPDLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5M5</accession>
    <name>A0A5B0M5M5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5M5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="59" mass="6119" checksum="B64AFF78E8A264E2" modified="2019-11-13" version="1">MVDLNTTTSNQLILGCSPGDFSPASAPAHSPSPYPALLLAFPTVDQPPLDSRLPSTTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M632</accession>
    <name>A0A5B0M632_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_050311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M632"/>
    <dbReference type="OrthoDB" id="2505930at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="34171" checksum="7E6A65D5B6F8884F" modified="2019-11-13" version="1">MELALFDWEKAYRQIPTQMDQWKYLLVKDFDGNFLLDTRITFGGVAGCGSFGRPADAWKQIMKEHFKLLNIFRWVDDNLFVRLQGEKISMEEVVDMSSRLGVLTNKEKYSPFQDEQKFIGFVWNGVQKTVRLPDGKVEKRISQITPFLESKANFDYEDVEILVGRLNHVAYILPHLKCHLCSLYRWLISWRIRKARRATPPDAFQDLTLWVDTLKSFEHTRIINYGPPVDVGWVGDASTSFGIGILIGKRWAQFKLQDPQSNPLRISYLETVAIRLGLLMILKLRNQRGKTLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7X2</accession>
    <name>A0A5B0M7X2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031586</name>
        <name evidence="3" type="ORF">PGTUg99_009357</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7X2"/>
    <dbReference type="OrthoDB" id="331341at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039905">
        <property type="entry name" value="CD2BP2/Lin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138:SF3">
        <property type="entry name" value="CD2 ANTIGEN CYTOPLASMIC TAIL-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138">
        <property type="entry name" value="PROTEIN LIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="457"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="62735" checksum="BEB0AAB98DE606DC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAC2</accession>
    <name>A0A5B0MAC2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="1">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_025557</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAC2"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF937">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="3">
        <location>
            <begin position="189"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1073785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="562" mass="61996" checksum="EEDD25FD0A34757F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBR7</accession>
    <name>A0A5B0MBR7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBR7"/>
    <dbReference type="OrthoDB" id="10277102at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="216" mass="24438" checksum="79BBBC9FEF99D596" modified="2019-11-13" version="1">MLFNKILIAFQLLHYHSLSARPLSLARHLVKREAGGRFNLPMVPFPLDAPIDIHRPRITITELDDSEEVGHRATARAPNNIQHPRITVTEPDDSAQAGHQAGADSLALQVYNPHAPRVTVPGDIKDIHEDFSAKMEIVLPRIKANHGDSVYQKVEEYYRSILQNRSTSPTFHPFEEHPQNGASLSQNEIKANQLKNLMQRAYMKTVKDHEEVLVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MD25</accession>
    <name>A0A5B0MD25_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Elongation factor Tu</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TUF1_1</name>
        <name evidence="11" type="ORF">PGT21_008010</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MD25"/>
    <dbReference type="OrthoDB" id="2067at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03707">
        <property type="entry name" value="EFTU_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000001">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000003">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000766">
        <property type="entry name" value="PRK00049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009372">
        <property type="entry name" value="PRK12735.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009373">
        <property type="entry name" value="PRK12736.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF36">
        <property type="entry name" value="ELONGATION FACTOR TU, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 11" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="87"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1074501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="478" mass="52413" checksum="7EE9FD2B2E029308" modified="2019-11-13" version="1">MSSICRNTILKGIAIKPASRTSSLLSGSLSSRLGLQCRAVSSSTSSGLSCIQSRSRSSRNPRALLPLHSLASRSYAAEASKKFTRNKPHMNIGTIGHVDHGKTTLTAAITKSLAALNSNNKFLDYSQIDKAPEEKARGITISTAHVEYETENRHYAHVDCPGHADYIKNMITGAAQMDGAILLVSATDGQMPQTREHLLLARQMGIQKLVVYVNKVDQIDDPEMLELVEMEMRDLLTSYGFDGEQTPIVKGSALCALEGKSPEIGVESIKQLMKATDEWLDQPVRDLDKPFLMPVEDVFSIPGRGTVVTGRVERGTVTKGTELELIGLGMNQKVALTGIEMFKKELERGEAGDNMGALLRGLKREQIKRGMVLAAPGSMKAVTKFLASIYVLTKDEGGRYTPFMNNYRPQLFLRTSDVTVSLTFPEEVKDRHEKQVFPGDNVEMICELVHQVAIEPGSRFTIREGGKTVGTGLVSRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDN7</accession>
    <name>A0A5B0MDN7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDN7"/>
    <dbReference type="OrthoDB" id="2500617at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="239" mass="27403" checksum="F923A828E8F1C610" modified="2019-11-13" version="1">MLDINLLLRIRPSDRSLDLPDCSRGDGERSAPEEPPEEGPQSQERIRRLTETILAVDRPRMVSGTLAEDASSGKIPEFKFIHLFRSSSHSPHDRQPWFQWLDHINNIDQHLDMITLDSIPNDLLQAEQWSSQRILQKPEPLPLNRDSSDSFHPTHSKSPSSDKTLKSPIQEHQTLEKRDENHQQSAAEHQPHEIRFDTINAGLGLTECNLMESQLGESYELDDITSFVRAEDQDYLSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDW3</accession>
    <name>A0A5B0MDW3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDW3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="150" mass="16357" checksum="27769CAB6001AD46" modified="2019-11-13" version="1">MTTMMMIVKQANKAVDPSASTRGYIGVVESHEGLGRRVLGRRIQRVVDTAKADDFTVSSVVARRFSGADPSGCESGLKTDGSSASFASWFPIVRSKLMDWEPQQLRWMAVMLTSSKRSCGPDDQTERLFDQLLLGGFGGFLFEGIPGGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ME14</accession>
    <name>A0A5B0ME14_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_034744</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ME14"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000103">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.18, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="668"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="7">
        <location>
            <begin position="101"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="421"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="517"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1075397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="688" mass="77869" checksum="6B384C6FAD0FE19B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFX8</accession>
    <name>A0A5B0MFX8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027522</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFX8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022685606" evidence="2">
        <location>
            <begin position="21"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="637" mass="72870" checksum="ADF025FE62D4ECC7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGN9</accession>
    <name>A0A5B0MGN9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGN9"/>
    <dbReference type="OrthoDB" id="2507241at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="295" mass="32994" checksum="C0BAFED10121B498" modified="2019-11-13" version="1">MIGMDVIGRRSVLNRPVHYLSLQKTLALEIANPFVKPHLKYYPEISAGQPVSRLSQSRKWLKELGPNTRAPMVRDGGRDFYLHELVQLKSSLIVVPDFFCELDGEMYALCQTPDIQICNETGQLMFMITKNPPSDSPKLARIAVAEFGVDYPSMTVDGGGLMSDLCGNRMYGEPMQLQTSLVMFLLPFILTMPADVELGTEREVIGPIFNRWRLKAAGRAIRHMPICLYADDTSGNSSKKWNKHISYYFTLAGLPPKLTNQHFNCHFLSTSNSAGAMELAEGIVDELMCAQSSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MK81</accession>
    <name>A0A5B0MK81_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-mannosidase A</fullName>
            <ecNumber evidence="6">3.2.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Mannanase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PGT21_011592</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of beta-mannosidic oligosaccharides of various complexity and length. Involved in the degradation of polymeric mannan and galactomannan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 2 family. Beta-mannosidase A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.25" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MK81"/>
    <dbReference type="OrthoDB" id="2866996at2759"/>
    <dbReference type="UniPathway" id="UPA00280"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004567">
        <property type="term" value="F:beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000200">
        <property type="entry name" value="Beta-mannosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001511">
        <property type="entry name" value="Beta-mannosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002310">
        <property type="entry name" value="Beta-mannosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000084">
        <property type="entry name" value="Beta-mannosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054593">
        <property type="entry name" value="Beta-mannosidase-like_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050887">
        <property type="entry name" value="Beta-mannosidase_GH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041625">
        <property type="entry name" value="Beta-mannosidase_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Ig-like_GH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041447">
        <property type="entry name" value="Mannosidase_ig"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43730">
        <property type="entry name" value="BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43730:SF5">
        <property type="entry name" value="BETA-MANNOSIDASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22666">
        <property type="entry name" value="Glyco_hydro_2_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17753">
        <property type="entry name" value="Ig_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17786">
        <property type="entry name" value="Mannosidase_ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="beta-Galactosidase/glucuronidase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022769026" description="Beta-mannosidase A" evidence="18">
        <location>
            <begin position="24"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-mannosidase-like galactose-binding" evidence="22">
        <location>
            <begin position="44"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 immunoglobulin-like beta-sandwich" evidence="19">
        <location>
            <begin position="382"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Mannosidase Ig/CBM-like" evidence="21">
        <location>
            <begin position="823"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-mannosidase Ig-fold" evidence="20">
        <location>
            <begin position="928"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00021795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF17753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF17786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF22666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAA1077547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1015" mass="114191" checksum="7A8168BEE94F3967" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP68</accession>
    <name>A0A5B0MP68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_032055</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MP68"/>
    <dbReference type="OrthoDB" id="10323519at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022710529" evidence="3">
        <location>
            <begin position="24"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1078262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="177" mass="19630" checksum="0A91117C19F3A827" modified="2019-11-13" version="1">MSHKASNVFSWLLLLILVHKYSTMELSQAKTHPDAVSAPIPMKVDMRPFIARENLGSSEALKELSGSSDRLEDHSNSQGEKTNSVAINIEDETVQDGSKGKPMANLPGKPSSESDGVIKSANEQKSKVELMIIDNCPKWMTPRRLLLSSIFSVLFPTILTIFFKTLEITNHGKRVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPS8</accession>
    <name>A0A5B0MPS8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033240</name>
        <name evidence="3" type="ORF">PGT21_034312</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1072367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="60"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1078383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="12544" checksum="5EF8BA8AC4BB28F0" modified="2019-11-13" version="1">MTASSIEPVENHGLKNHINLQSMNISVSLRRKTTASEEGGNKTQTFIYVLLGNGQQASSTWARSDPIQNIRTRPTQLRSHHGARTPAGLAAESDDKPPEVFMYCSSPGAGSAPLLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPT3</accession>
    <name>A0A5B0MPT3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-dehydrosphinganine reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_025399</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPT3"/>
    <dbReference type="OrthoDB" id="7289984at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391:SF14">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY PROTEIN 7-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391">
        <property type="entry name" value="RETINOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1077969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="42199" checksum="DBD2ED341F31155B" modified="2019-11-13" version="1">MRTTTNPDELPKASHPPSTSTIAINGLAPPEFPFKRLGSLPSLSYALENLIISIGTIITAFLDCILTLYVWIPLGLARSLLQNLIQPFLRAISSQQQQPHDSSSSDQHHHPTSSPSSSNPRVIILSGASSGIGASLVQTYAGPNTILVLLARNPARLNQIAKVARNAGCRAVETHSIDYSHDDAEQAIKLVITNAHQKYGSIDLLLSVTGTVTFTDDDPLGPQQWGENTVKRLNKINVTSTYTFIMTAWELMKKQRSGNICILSSSVAFQGPPQFAVYAATKANLLSFSQSLRALSRPYGIRVSCICPGFIESGMTGDMLAAGSSMPSFGLADPSQMAQRIKLAVDQEQPGVIWPISHVLPLVMASRLNWLNAELARWVASKIGVTGQMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQP3</accession>
    <name>A0A5B0MQP3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQP3"/>
    <dbReference type="OrthoDB" id="2497193at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="193" mass="21325" checksum="644982EF1DC71E32" modified="2019-11-13" version="1">MDGIAAGAPYHSKFSNEATGPLPRAPRVPMNKKLKMICALIDNLNLTPKSFIIAFLEQDDDSMAFKRRLWATDQGWDSTEALLLAIKHLCCSHVAGRALWEEFILSQAIEIVSAQRPVSGMAPQGSYHTSATLSDAFFTKEERDHRNTALINRMPFLYRLLCSKLQGNMPGVHVGSVAPQDPVQVLRNTSGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVQ6</accession>
    <name>A0A5B0MVQ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005742</name>
        <name evidence="2" type="ORF">PGTUg99_024864</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVQ6"/>
    <dbReference type="OrthoDB" id="2508626at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="206" mass="24066" checksum="E72DF7873B3BFA31" modified="2019-11-13" version="1">MGMPFNGPKDSKDVVKETRDGPGAHTRVTRTPTTFNPVPKGKTISLKQFRIIAPRVTGINPVDIRRILRGFESRSHRNQRFAVLSEIIDVVLSWMAASEHPRLPEIASKGHKQILEEWASQFDYMHFDEMLEYSRPELSGTKKTYIKRFALRNGERNEYHLATQTHWSHAKVEALYEEWKASHKSFKFHVLDFWEGFLSLFKRKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXT2</accession>
    <name>A0A5B0MXT2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017021</name>
        <name evidence="3" type="ORF">PGTUg99_003224</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000378">
        <property type="protein sequence ID" value="KAA1092037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXT2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137323" evidence="1">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="52" mass="5528" checksum="8C9D46A6448194E3" modified="2019-11-13" version="1">MSLITILVVGLSTVETLNARDLNSSTIIVIGSLSTSIIRLDSSWKILAALLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZF9</accession>
    <name>A0A5B0MZF9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZF9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023076557" description="MYND-type domain-containing protein" evidence="1">
        <location>
            <begin position="34"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13270" checksum="F7368971EF194FDC" modified="2019-11-13" version="1">MVQIQTRMTFISKLGQFCAWLLLISLIIGSALCEENQLALKKLETPENSSTISDLKPVSLRRRSPLPQATQCPPGQGLNKKKKCAKCGRKQEVVKNRCVKKCKNNQTRNAQGKCVNKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZI6</accession>
    <name>A0A5B0MZI6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_11</name>
        <name evidence="2" type="ORF">PGT21_000250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000127">
        <property type="protein sequence ID" value="KAA1081743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZI6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="324" mass="36973" checksum="232B97E27A75314C" modified="2019-11-13" version="1">MNFGIPATASVKDMQLLHNLWCSSGDNLRKILEETRMRGLITHAPEFFKTQLENLDLVNRFCKTLELKISESLKAGTSNSQTTKHQSNPLRTPSKQPRSQQICPNQPENPSLSPDTDHLAQVHSAAPNQPPGTIETIPTRQIPNHSPLSASPSPARTKPKKKSPRKRKSSIQDNTAPKEKRTRRPLTYFEKEERARKAAQRKDQRLSSVHAEVMCPADLMDKFWEKTKCDEAREEHKQMLIKSRNPRWDYGPPLMSLVIDMIEWFADDTFADSFYTNLDLFNPSAEAMEAFRAVLNPAFKHCIAQSPCLLQVDSRDRILSRKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZQ9</accession>
    <name>A0A5B0MZQ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZQ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="88" mass="9439" checksum="0EF387215A370B77" modified="2019-11-13" version="1">MRRQQDSSDHFVFLPSFARFRTNSAACPLNSTPGCSASHRSLQESVFVVVAGIQATLDKRSIRNEIAAGAISTTGSSSNSSINFGLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N072</accession>
    <name>A0A5B0N072_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N072"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="11373" checksum="5DCD6BE84F964E54" modified="2019-11-13" version="1">MFGRKFPRGERHWETTKSNTHKLFNIATTESNAQYIRKGRKYGLKDTINNKFIIESKSDPQVKERMENFAQGSSHRLYNPILELIGFDGVSPKPNDTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N279</accession>
    <name>A0A5B0N279_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012160</name>
        <name evidence="2" type="ORF">PGTUg99_029237</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N279"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="74" mass="8255" checksum="944C373D8C3CD9CA" modified="2019-11-13" version="1">MSDENFSHLRARGSRAYFHVEDETLMIKSRTGDSDPVGAGADQGMRFLLCPSMDQNRSPPDLTKHIPHGYPLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2D1</accession>
    <name>A0A5B0N2D1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010551</name>
        <name evidence="1" type="ORF">PGTUg99_031832</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2D1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="110" mass="11907" checksum="647B04220D353BDE" modified="2019-11-13" version="1">MAGHLPHQNIPACSDERAQICHMKLVHVGFCGGVSSALADVTKVQEHQRLGLLAKLSGWAWKPPCSPLEHKPKSCSGESVYSDYLVPHSALVFPSTSDFLLSLDAPEPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2S7</accession>
    <name>A0A5B0N2S7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_002601</name>
        <name evidence="6" type="ORF">PGTUg99_031380</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2S7"/>
    <dbReference type="OrthoDB" id="2506344at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052416">
        <property type="entry name" value="GTF3C_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="406"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="767"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1082420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1133688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="894" mass="98044" checksum="94D41F2539C32990" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NCP1</accession>
    <name>A0A5B0NCP1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019696</name>
        <name evidence="3" type="ORF">PGT21_020030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NCP1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="62" mass="6543" checksum="7F5898C6521C7DB3" modified="2019-11-13" version="1">MPPPRYPPDLVDPPQLAHRQSFEAKARLARSTRWLGSSTAPSPPSVVPALAAPASVSPVPMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND54</accession>
    <name>A0A5B0ND54_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023688</name>
        <name evidence="3" type="ORF">PGTUg99_012184</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND54"/>
    <dbReference type="OrthoDB" id="2508874at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="120" mass="13325" checksum="525B1E1E5239DC60" modified="2019-11-13" version="1">MSVTDMSFSDFKTRVYNHLAATLDHSLLKSEHLVKALTNADKANQLQWRCSIKNCERVVTDDASFKLFVVVAGIQFGACNVLLFMDKPCRSNQFKLSTASGQAVRFAGKENSNSDDDISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDX6</accession>
    <name>A0A5B0NDX6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016362</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDX6"/>
    <dbReference type="OrthoDB" id="2498779at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="127"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="363" mass="41658" checksum="218FADA743A1D071" modified="2019-11-13" version="1">MRGYKGEGGKSKRRRKKPKTLKGQAVSQETERASQQFIESMQFQPNQPNSSQLSQDPYFTGPLNNQNHEQSVGRITPDPVDPQVETLSQYFQNQHHEHRHVLEEQQWADVYGPMFSSFYDCAAKTANWGDIKKWNSDWKPTCGCIPTRQRPVVLVDILSRQEERIDFCPCQPDQVRLIQMGYIGATPKFPKTAFSIRLLQLHHILWKHCTIAMLPFSKALDEFLDINNPLILVPHGEDDLEESVSTRHWRKQLSKAVDAFREMLKREKELVAQVMKLEPLGELADICPKCYGPQVDGKREGEPDYILCMDGNFQHRRHLKASVEHSENIETPSLFVQPCEVSEMEESLAQLQQPSGTMEGSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFW0</accession>
    <name>A0A5B0NFW0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011010</name>
        <name evidence="2" type="ORF">PGTUg99_002303</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000407">
        <property type="protein sequence ID" value="KAA1088097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFW0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="52" mass="5577" checksum="93F998393859E027" modified="2019-11-13" version="1">MLSASAASIPPAPKQTRQSFGQGALSPPDLCHVPSPESEVYDHYNATRNHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI06</accession>
    <name>A0A5B0NI06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI06"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="85" mass="10266" checksum="3E9D5D1AC41C706E" modified="2019-11-13" version="1">MKMERDAAELRASEAQEDRKRERQEALDRRDNEKKREDEREKRAAEERKRIDEREERQQQSRQLFEMAMLKAMGMSLPSAPEKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMZ7</accession>
    <name>A0A5B0NMZ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMZ7"/>
    <dbReference type="OrthoDB" id="2505966at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032801">
        <property type="entry name" value="PXL2A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630:SF3">
        <property type="entry name" value="PEROXIREDOXIN-LIKE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13911">
        <property type="entry name" value="AhpC-TSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="618"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="625"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="674" mass="75347" checksum="D3DC869F0DA405D6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NN92</accession>
    <name>A0A5B0NN92_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Eukaryotic elongation factor-2 kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">EEF2K_7</name>
        <name evidence="9" type="ORF">PGT21_013945</name>
        <name evidence="8" type="ORF">PGTUg99_011719</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NN92"/>
    <dbReference type="OrthoDB" id="2923034at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903013">
        <property type="term" value="P:response to differentiation-inducing factor 1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF2">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="7">
        <location>
            <begin position="312"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="209"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="216"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1065376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1090791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="582" mass="64549" checksum="CD3FA36953CDCC92" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPW4</accession>
    <name>A0A5B0NPW4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPW4"/>
    <dbReference type="OrthoDB" id="2504287at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="233" mass="26359" checksum="83B809EEC31D904D" modified="2019-11-13" version="1">MDLDVTTSDPTEIMGSDDATTPPSSPPPPIILTNDDKRDILASLLQEGWKGPDILIILQENYGWTISLRSLSRLRQTWSLRLKDLPPQDSLLPATRASLISAHRNGLTVFEMRSQLLSETGMDVSTRTVERYLRVLGLKQRQNDLADGKITREGVVEHIRHARDALLANSAGYRRMRQILVTNYGLHVPRWYMTFSKSWILKEWKCTLKRHAKEESSGPLVPITSGLRMGTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NT45</accession>
    <name>A0A5B0NT45_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003610</name>
        <name evidence="3" type="ORF">PGTUg99_018958</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000312">
        <property type="protein sequence ID" value="KAA1105153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NT45"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="98" mass="11553" checksum="C666BB122FF58220" modified="2019-11-13" version="1">MPKPSPKPIPTPIPLPDGIGSPPPLNRIDHRPVPFVEVNKINRSEFFGLGRKVVLIFFPISKHPRRCQKRIQYRYQRRRRRKIPSTSNQLNRSTTRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NT92</accession>
    <name>A0A5B0NT92_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NT92"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10691" checksum="F91CF4C8B3236FE0" modified="2019-11-13" version="1">MTATTSEKRQTSLGHGGEKKKKICDSSSDRRISKEAASYIPEEGRPPKDLDNWILFEANVGAHSKEADDYIPEEGRPPKNLDNRILLEEHSQLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTS1</accession>
    <name>A0A5B0NTS1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ataxin-3 homolog</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Machado-Joseph disease-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PGT21_011541</name>
        <name evidence="23" type="ORF">PGTUg99_029912</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 24 25" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">21-0</strain>
            <strain evidence="23 25">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as a chain editing deubiquitinating enzyme that binds and cleaves 'Lys-48'-linked polyubiquitin chains, with a preference for chains containing four or more ubiquitin molecules thereby modulating protein degradation by the ubiquitin-proteasome pathway. Probably by regulating the IGF-1-insulin-like pathway, regulates lifespan. Regulates germline DNA double-strand-break repair and apoptosis in response to DNA damage by recruiting E4 ubiquitin-protein ligase ufd-2 to DNA repair foci. Interacts with key regulators of transcription and represses transcription. Acts as a histone-binding protein that regulates transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 26">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Forms a complex composed of deubiquitinating enzyme atx-3, adapter ubxn-5 and cdc-48.1. Forms a complex composed of deubiquitinating enzyme atx-3, E4 ubiquitin-protein ligase ufd-2 and cdc-48.1. Interacts (via RRDR motif) with cdc-48.1 (via N-terminus) and cdc-48.2 (via N-terminus); the interaction with cdc-48.1 is not required for atx-3 enzymatic activity. Interacts (via C-terminus) with ubxn-5. May interact with ned-8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTS1"/>
    <dbReference type="OrthoDB" id="10063692at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000018">
        <property type="entry name" value="Ataxin-3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033865">
        <property type="entry name" value="Ataxin-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006155">
        <property type="entry name" value="Josephin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159:SF0">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02099">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01233">
        <property type="entry name" value="JOSEPHIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01246">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50957">
        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="21">
        <location>
            <begin position="4"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="209"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="266"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="266"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="18">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="active site" evidence="18 19">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR633865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAA1092697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAA1093796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="365" mass="40491" checksum="C5A6E02BC9C0C5BF" modified="2019-11-13" version="1">MDLVPYIYCEKQEPGSMLCAQHALNNLMQSPVWSPQDLAEIARTLDSLERSHLQHDGGTHDTSIQGSSRLEKNSNYDDSGFFSVQVIDDALHRLGLRIIRWRSEEMKSMHDYPENQEAFILNHDLHWFTLRRFGHSVDRWYNLNSMDEASPKWIGPTFLSMAIAQAEAEGYSIFVVIPVPPSSEPSAPQPGSLLACQADEQAQLLDQLGYSSGGRSGSTSSGGASSTLRRPREEGTDEASRTRMRMNTEELPYDEQIRLAIEASLSQPSANEPSQTKNIEPQTAPDPSDSENNGEPPADSDLLDQLRSIEDEELAAAISESLANHSSESDPTEPPSSSSLPPVSDDLIDEPTPEQLRQRRLARFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYN4</accession>
    <name>A0A5B0NYN4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NYN4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="59" mass="6508" checksum="DAD04AC767E76B21" modified="2019-11-13" version="1">MLSNLGDAWDAVHIGGGDEGEEENVAPLTNSESSRRRAEAFRNHVQEKCLAFHRNQGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZL5</accession>
    <name>A0A5B0NZL5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZL5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="462" mass="51727" checksum="EF698548CF8EFA11" modified="2019-11-13" version="1">MLNINDLIHGDPDDPATRLEGAEESHYDTSSDDHDSPPSNHMEITDPNNMFAALPSYDVNMTDRSSAPGAEKNVEQICSDLQSKLMLDPEHLEIALIGSKASPEARHASLVFTNGAYHQLGNKGTQKPSFVYDDTFRDFVRTSARMFLLKPTLEAYSNNPHQNTDIPKTLFYLTLDAVEKQPDAWKDDHLPPRGVRANVEVLNHYREVVGELLKYQRSNLRTLVRYFSEFFSRSMLILLSQLLTEILVTKKKPFVSKMPNRKEMLTMIYKDLPPKASKLTEKEIKHQVHTNFAMRARMCYARLVMVYYYIHKSKKESQWIEIDERLDLVLEKDSELFSDKKEFASVQHQDLTVPTIEDVRIALAGAHSTGEAVGSGLTCGTEDSPDISPVMAPIPTAGTHGSPALEASRRRVFHVVPSWVGWANGSSRHLPHRNTPTDQGGVGRVGSTSGLTSAPSCTKPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0U2</accession>
    <name>A0A5B0P0U2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0U2"/>
    <dbReference type="OrthoDB" id="10357450at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="137" mass="14537" checksum="E8A06882CF740B44" modified="2019-11-13" version="1">MFDLPQLPEAHPGANSFGRSNEDDRSDSDHNALRTHCDGEHSTEAPSVPVPSTPENSLRLSSSGRSHSHEDSTVHTSTTDSRTPPGSPGSQGTTRAPLPSAVSSESDPTGVIDPGADDYKLRQATSNAIRMLFWDGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1R3</accession>
    <name>A0A5B0P1R3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GTPase of the mitochondrial inner membrane that associates with the large ribosomal subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MTG2_2</name>
        <name evidence="6" type="synonym">MTG2_1</name>
        <name evidence="7" type="ORF">PGT21_032660</name>
        <name evidence="6" type="ORF">PGTUg99_002865</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1R3"/>
    <dbReference type="OrthoDB" id="347018at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="entry name" value="GTP1/OBG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF31">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="5">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="185"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="239"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1065215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1094916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="456" mass="50521" checksum="2D8DDC4020BA4EC8" modified="2019-11-13" version="1">MNGPAGKDLIVDVPIGTVIREIRSSQISAQRESALGFNLGHRPPKASDLVGEDIEVAKARRSKLFVHYPESEDSNQTNEALKNLEIDLLQEQWATERAHSQSEPFQMDCAKFHEDHRSRTNIEHPSTQVNRSIKAEQDFFLIAKGGEGGLGNSNFSGNQTTLPRFATRGKKGEVIELELELKTLADIGLVGFPNSGKSTLIHTLTNSRAEIAPYPFTTLNPQIGTLIIFNDGSWDLDEPTDAINHSPSHREYLDANSATDLIRDRSNRQRSSSHSPRKCESIRLTIADCPGLLPKASENVGLGHAFLRHIERSRMLVVVVDLMAGLPTATTTTTSSSSSTNQRLIEDENPQRCCQDVETLFKELESYQPGLSGRVGILIANKADLSSTDPRLQEIAQVRLQALQDYVRALSSFQVQQSIRNLGTPHISVLPLSAKFRLNVRKLVLVIKEFFEASKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2X0</accession>
    <name>A0A5B0P2X0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_028853</name>
        <name evidence="11" type="ORF">PGTUg99_029600</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Vesicular transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000371">
        <property type="protein sequence ID" value="KAA1095771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2X0"/>
    <dbReference type="OrthoDB" id="440553at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17325">
        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF23">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="89"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="177"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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        </source>
    </evidence>
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        <source>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1087321.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1095771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="516" mass="54821" checksum="21A947F204510098" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P636</accession>
    <name>A0A5B0P636_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032393</name>
        <name evidence="3" type="ORF">PGTUg99_005880</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        </location>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAA1096955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
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            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="281" mass="30859" checksum="B2AEA36840FC33C9" modified="2019-11-13" version="1">MDNPISPMMTSSSLPSDSRSPSQPRSPWSSLISPTPNRARFPAMVLANLIRHKPGHNSHTNNPLPSPSLANPTTPYSTTPGEDLNNFVWDVACSSQSPRRAHEEEELYEIARMYQPSIGKRTRPTPQPIVTRRDLLPLATVVPEGALTPTHSSAGAFKSSIHRLLRKRTALHLKNSSQSSSSTTSPSETSADAEDRMMDPELEETSDDEYKNRSAYLPYNIHNDTSPFSSQQPLTPSLPTHAGFSSPLSAPRTPARERRASPQDAQFLCNEVPPRSSSIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0P7K6_PUCGR</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">PGT21_021415</name>
        <name evidence="2" type="ORF">PGTUg99_030629</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="KAA1096580.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="84" mass="9777" checksum="6020E8CE4A405AC0" modified="2019-11-13" version="1">MDHCLPLYLLPHPSSIIVRDFSTFLVYRPNAPGTVVRHAHQKLNSSATYHIPFRRFRNLRGYTNKTCSTKLNLKLTIKHKSNMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7W9</accession>
    <name>A0A5B0P7W9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clock-controlled protein 8</fullName>
        </recommendedName>
    </protein>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:phospholipid biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TF_Opi1_Ccg-8"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38406:SF1">
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    </dbReference>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="648"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="77429" checksum="88DA5685ECE1F9F8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P881</accession>
    <name>A0A5B0P881_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P881"/>
    <dbReference type="OrthoDB" id="1922221at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="313" mass="34885" checksum="37162E633D7DB68E" modified="2019-11-13" version="1">MLALDYVRGGMPLMSQLISSSPITTRPSIKRCSSKTVLTPMFISSLDEIVKSIQTLSRTEAPVSESTLHAEKLLRTGVESLSDMFAGWVRDSTGPPLTDPVHQTADPPQPLGFSPNTINKLHKLYAYLQQLSKSLPSNQTIQDVHKEIVSAYASVRSKSNMKRFYLSSKVLARSKSTRSTETGQSVNSVIKRSLSSYIGVAFNTYAAILEQLPRFDEEVRRPAGRKENELGDLLHAFKGSCLRSLPEFIADTKTFWEKQPVGSEASNTMTSEMTIVAVEYLKMFSVMENGSLERRIIPRLPLVLARLTMKHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8Q1</accession>
    <name>A0A5B0P8Q1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8Q1"/>
    <dbReference type="OrthoDB" id="10630630at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="154" mass="17322" checksum="9C6D74039E585EBE" modified="2019-11-13" version="1">MLIWDKHPFQGWDQLPGPYIFFRMAARASPMDSLNRDSRPRFSESCRFSGSNGLANRLDKAIQGHLDSPKPSAFVESPPNPTTQPTPQIHCPHRTNFNFDMPSSNNKVKIIDPALATMVEVVDVESSAQKTLVAKEQSDKNNKNKCDLKQKKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9Q2</accession>
    <name>A0A5B0P9Q2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019043</name>
        <name evidence="3" type="ORF">PGTUg99_011850</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000173">
        <property type="protein sequence ID" value="KAA1126017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9Q2"/>
    <dbReference type="OrthoDB" id="10271090at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366354" evidence="1">
        <location>
            <begin position="21"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="133" mass="14950" checksum="4976B9AF0E5792FC" modified="2019-11-13" version="1">MKASSPWIFLIMLNALLVNGMVERSAKVAAVSWPKQWLLPPYEAQPGHALHIPRAKTLFIWENHYIKFDAGDAHTWTVFNPMDCEVLITAHFKHPNGQDSKTDFTFPANSTSDLNIPLTTELIIDVVSVDGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCK7</accession>
    <name>A0A5B0PCK7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCK7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="57" mass="6644" checksum="83B19571B8D7944E" modified="2019-11-13" version="1">MYSTTRQPIGFRSYDRPTGVSTYPIRSLIILHQGALYFRPSSLHRAKQPDHEQRLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PE48</accession>
    <name>A0A5B0PE48_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PE48"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="68" mass="7707" checksum="248DEA25F6E96843" modified="2019-11-13" version="1">MREEIKVPPSERSRRNLCQAQRITPDVPCIKPDFTITESSTGMIQPCSIQSKKSHGELLHWTSQPPTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHK3</accession>
    <name>A0A5B0PHK3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_050002</name>
        <name evidence="2" type="ORF">PGTUg99_050089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000482">
        <property type="protein sequence ID" value="KAA1070923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHK3"/>
    <dbReference type="OrthoDB" id="10431591at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="412"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="61278" checksum="3593F91AA6AD852F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJB2</accession>
    <name>A0A5B0PJB2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_050201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJB2"/>
    <dbReference type="OrthoDB" id="2507389at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09276">
        <property type="entry name" value="Rnase_HI_RT_non_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33481:SF1">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33481">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="324"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="600" mass="66687" checksum="3B944BEBB212D7D8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKD4</accession>
    <name>A0A5B0PKD4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_023619</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FUN14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKD4"/>
    <dbReference type="OrthoDB" id="10378063at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346">
        <property type="entry name" value="FUN14 DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346:SF10">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023083400" evidence="6">
        <location>
            <begin position="25"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1101526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="130" mass="14702" checksum="DD295F582A98BA74" modified="2019-11-13" version="1">MKQQITVLILLAITAYQLTKPAIGIDAALPPQMFDPQPQQPIKHKPEEFSSLLSVQKFSFGTLTGICAGVFVKKGLNFLAFLFGGGFVLLQINWKTWANQYESKFWLAKEPPTKSIVARFMDFLRSDFPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PPB1</accession>
    <name>A0A5B0PPB1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_000393</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000052">
        <property type="protein sequence ID" value="KAA1102470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PPB1"/>
    <dbReference type="OrthoDB" id="3819888at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF4">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_7G04540)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1102470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="26987" checksum="736EFE53F7DE7879" modified="2019-11-13" version="1">MCTQALAAHGARVYITGRREEVLNQAVDNYHKHAKGEIIGANVTEKSDLEKIIKDISEKEPNGIQILVNNAGISGPKTEVFSNGHSIEEASQKFLKNESFDQWDSVWRTNVSGVFFTTVAFLPLLAKGGDNNMNYRSGVVNITSISGMVKISQNHFAYNASKAAAIHLTKMMACELANSKIRFNSIAPGVFPSEMTAKCGSDDRGKSDISESFDASKYKIPAGRPGRLEEMAASILYVSIPSPDISHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQU3</accession>
    <name>A0A5B0PQU3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NAD-dependent glutamate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">GDH2_2</name>
        <name evidence="6" type="ORF">PGT21_005931</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQU3"/>
    <dbReference type="OrthoDB" id="184415at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056365">
        <property type="entry name" value="NAD-GDH_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028971">
        <property type="entry name" value="NAD-GDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055480">
        <property type="entry name" value="NAD-GDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF24">
        <property type="entry name" value="NAD-SPECIFIC GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05088">
        <property type="entry name" value="Bac_GDH_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23147">
        <property type="entry name" value="GDH2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23152">
        <property type="entry name" value="GDH_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent glutamate dehydrogenase N-terminal" evidence="4">
        <location>
            <begin position="49"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="NAD-specific glutamate dehydrogenase second" evidence="5">
        <location>
            <begin position="124"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="NAD-glutamate dehydrogenase catalytic" evidence="3">
        <location>
            <begin position="520"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1103082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="720" mass="80840" checksum="331A0BD2BAAF66F6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRC2</accession>
    <name>A0A5B0PRC2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRC2"/>
    <dbReference type="OrthoDB" id="10361277at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="397" mass="45878" checksum="F9E28E34FCC3F895" modified="2019-11-13" version="1">MMAEVDIASHDLMTKETSRTEPKQESTSHTLLVPAPMRIKASSDSPRSLIRLRYLWTPPPPKLRTASYLRGLRLKKEEFIRNYKPTDWELLSQEERLQIAIARIHREQDNTLAAQLLINETIREFKEYKKERRIIEMNSVSNATSANQVTGANNPINNPANTFSPENIGNPEDTLVDKLEDLLNCPRDQTSSDRSQLLLGQRVEQPLEERVRQLSRLQIKKKTIETIESPTKTNHANLSDSQNKRNNEAMDLDDASTSSEDTQKRQDSPEIMTMTKKEKICFLVKEHVAIWKNFEKEKASGVTNKLKNIIHQAQESQKVLQKLITKEELEGYVKGWNPWDAKRELFPPPPKKEGKKKSSTSRKAQQYDDPWVWADVFEIGQAWRAAYRQRSRKALPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRR2</accession>
    <name>A0A5B0PRR2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_012472</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRR2"/>
    <dbReference type="OrthoDB" id="1900170at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053031">
        <property type="entry name" value="Cuticle_assoc_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34396">
        <property type="entry name" value="OS03G0264950 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34396:SF27">
        <property type="entry name" value="OS08G0208700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="6">
        <location>
            <begin position="84"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1103280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="14050" checksum="A7A02B1980A683E6" modified="2019-11-13" version="1">MANTRSNSKRKRPKTVPDSEETSSANSSTDEPEVAVEHTNTDQIEAAKNPSQPEISASQTTVAASQNVDKSNLTDSATSSTERQQRSTVWKHFTKSGTGEETKATCNHCKKTLNGQSSQGTKSLWRHVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSA9</accession>
    <name>A0A5B0PSA9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSA9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="284" mass="31395" checksum="38C169D622F36F9E" modified="2019-11-13" version="1">MRKFIRSRLSAKASDESFCCCGKVEELAEEAYLSSKKSSSRENLRASECVPPPGDKENQPIASSSKGYTPPVFPTHRLYVPPSPRKQALRNSRSLGNLEAIRKEKRKVAQKHVAFVEDGPSERLSSDSTIPAFAQFTPASGYGRFKGTSLSSKDCLPSGIVCLPLRKQASLRVNSESETTIARVLLAQSKHNRAAEDSGKLVAKPKRFRVVLGKKAAQGEAEVINPQRHRLLDTSQTLTGLPKRAKESLYPSFNPTKPEKTALHLSDLRPFAPDWFIKFSEITT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT68</accession>
    <name>A0A5B0PT68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome P450 52A3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CYP52_2</name>
        <name evidence="7" type="ORF">PGT21_001395</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT68"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="599"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1103814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="671" mass="75683" checksum="BDA386872BB46F0C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTL9</accession>
    <name>A0A5B0PTL9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTL9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7844" checksum="FB326587A872D630" modified="2019-11-13" version="1">MSRSIVTRVQQLVEIDSYSGSTAIEINSYSDLTTGQDGTPLEFEGWSRWNTTCVQRLVKIEHHLRLMAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PV96</accession>
    <name>A0A5B0PV96_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032564</name>
        <name evidence="2" type="ORF">PGTUg99_016553</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1099009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PV96"/>
    <dbReference type="OrthoDB" id="2507317at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="636"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="484"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="712"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="733" mass="80193" checksum="3D30A11490CF16A4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0R6</accession>
    <name>A0A5B0Q0R6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">beta-glucosidase</fullName>
            <ecNumber evidence="4 11">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_036327</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="4 11"/>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0R6"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="UniPathway" id="UPA00696"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:004252">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000002">
        <property type="entry name" value="Probable beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1700:FF:000003">
        <property type="entry name" value="Probable beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF2">
        <property type="entry name" value="BETA-GLUCOSIDASE F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022850519" description="beta-glucosidase" evidence="13">
        <location>
            <begin position="28"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type III-like" evidence="14">
        <location>
            <begin position="776"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="729"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="741"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1106584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="856" mass="93620" checksum="A5BCD15441BF96AA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q344</accession>
    <name>A0A5B0Q344_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q344"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="132" mass="15068" checksum="1406882FC23DEDCA" modified="2019-11-13" version="1">MDPAEGFPSAGRGTCTSSKRKAFLPMDEWLKAADNYARWRCGDDKDALYRELLAEFISHGITHRKRCETNLRICSLQMSYNAGRNFLMANAVEPLDDPWVKDELMRICPHFHELDAFMEVGQCPPSAQQITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q3K8</accession>
    <name>A0A5B0Q3K8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q3K8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="55" mass="6002" checksum="4B6814920D0595E0" modified="2019-11-13" version="1">MEPVSAPVHIGSMAKLQAFVIATSKTELCRRNCGERLVGIGHHPDNQRDQTTQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q487</accession>
    <name>A0A5B0Q487_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032131</name>
        <name evidence="3" type="ORF">PGTUg99_008945</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q487"/>
    <dbReference type="OrthoDB" id="2500250at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="244" mass="27097" checksum="D3983D10789469C3" modified="2019-11-13" version="1">MSGYRQVQNVSSLIATVESLDGTATVFPRWRSRLEDVLGMQNTLNIVKGTLPRPKEDSKDITTSSSRPVDSKGYNPKESKEDWDALLEMACSTIRLTLTDPLAQRYRKVKPASRLFSTIVNAYEKNTRARRIRLQESFWTARHDPNKPIALWIGCLRVAADELLSIGELPTDRQIADRLVGGLDPSWSAVRDSIVYTAIEMSLDDTIGALEAHEVSLNGTNHQDLVSAASAKHFACSNCGKRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4L5</accession>
    <name>A0A5B0Q4L5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029690</name>
        <name evidence="4" type="ORF">PGTUg99_025136</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1108033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4L5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366498" evidence="2">
        <location>
            <begin position="21"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1108033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="141" mass="15546" checksum="0B5681F047643ADD" modified="2019-11-13" version="1">MIPTTILFGIFMAFIPAATAVGVKCAREGCKYRAYPTYPLNQDKYPPEGPCGGYLGNNMYCQNNRLKMYYDCKCGYMTVKNKINQEGKEPECAHKGKRLASTPSNSPPPVEASPSGASEASSSAREIPCKTINGYQYFEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4T7</accession>
    <name>A0A5B0Q4T7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4T7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="154" mass="16524" checksum="E60E58C937AA9657" modified="2019-11-13" version="1">MRISGCFFRPSEHPNPHPHPLAGIRRPQRVSERVSSDTCPLKGRLEGLPSSRQGTCTSLTGRDSFQPAISPSGRGYPLGYPDTRQLFAEKGPSAPKSAPVGGYPLALAGIRQRITGIRDGYPLTISICLPSYSIHTLVTTSETSLPYSFGMIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q567</accession>
    <name>A0A5B0Q567_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q567"/>
    <dbReference type="OrthoDB" id="10550145at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="178" mass="19223" checksum="F07528AD783F0820" modified="2019-11-13" version="1">MTILTRLSLRSSCGSPVHGTHGKQCGQQPGDSRRLQRWDGLSKVKGQTNQEEGALPSVESHGGLKDGIPAESERKETVNTIAANQSMSGASTTTVIGPSPETQGPNPKKRPIEEPDKIEVIHGPNPARLVHAHTYRAPLGPESAAMEAVDMETYLRVSHIEDADQATRRRLRANGITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q596</accession>
    <name>A0A5B0Q596_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q596"/>
    <dbReference type="OrthoDB" id="2510080at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022903299" description="Secreted protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="18479" checksum="0349BC63A0AA0EBE" modified="2019-11-13" version="1">MPMSRWTRRLSVITLIGFLSVGLSESLQHSLVRRQDTSRQQCGIGFGVEGLGPDITCINYNGDPYTCRTLDCYIGDPNSRPDLPPYPLLLFKNCRKMLSDYNLGPPINQTHPIWYHADNQAQTLEAQIDGSGDKYLCTWTESTDPNNVRPYCDGCSSADTENSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6Y0</accession>
    <name>A0A5B0Q6Y0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029783</name>
        <name evidence="3" type="ORF">PGTUg99_037657</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6Y0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="168" mass="18967" checksum="BC8342612362DCA0" modified="2019-11-13" version="1">MGVMISNQTWLKVNKAWRPSDQDNPTGRNTFKTLHHPCHSYPFSSACLVEEASIHMLRRDYPSLVKLGFFIPGMITLLLLCITFLNLPLIILAHGGSLSKLQTHQIVVRPNALRNFEIREPPVVPQSKACESRPAAFEIYEARSVYTCSSATAYHHLSSLTSPYQLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9S9</accession>
    <name>A0A5B0Q9S9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9S9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1109875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="142" mass="15934" checksum="B3B916AEA98E5130" modified="2019-11-13" version="1">MTYNKCLWILEAPEVISLSHLAILYALDWKPLKAASSLPPRDLVDLVVRGGRPRLSLPRPCSFREFRRSAAPPSPHPSQGDVYLSSTVLQYSIWSSLTQDLPVPPSPPPSQVTDNLSSQFKDHVIVKVIISHFMPNHTNLWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB89</accession>
    <name>A0A5B0QB89_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_019127</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QB89"/>
    <dbReference type="OrthoDB" id="2518641at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="112"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1110362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="369" mass="40807" checksum="821963D26558A3EB" modified="2019-11-13" version="1">MKNDPQDQVISQSVAQHPASHPIHNYRLPRSVDRYSVQGQPNKQYQAPLPAFVADHPLIDGVKHNKPGFNGPDACQDVHEEFGLGFDMNNKCDDLDNECPNNLDEHVYDLSEQEQASKTTSDNRDPEVLPKIVPESNDPQVPEPAFDMANYSAKDVNRWARTLSADQFEELRVLGPESRIESFRQYAISNLDRPTRTLSSSIHEHLEQLVPPSIGNKDNSDYSNCQPNDTDKNNSYQQQLNYQVDNFESNFYNDLDGHDSQLEENLDGSVGFAADNGLNDGSSFDNGGFDDGSFDDGVFEDGGFDDGGFDDGGFDDGGFDDGGFDDGGFDDGLTMAALTMVALTMAVLMMAMVPLINSLFHIKTLFLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBY7</accession>
    <name>A0A5B0QBY7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBY7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="121" mass="13548" checksum="4245A4FCC8276B4D" modified="2019-11-13" version="1">MDPTRLVTDAEEFQGRVERHVQSHEPYCDLQSDLMFMRTQSVISSQQNFVNPPPDLPQTSENLHPAEATYAPAKAHSSPPVIQSEVTEAAIIRTPPLASIDPTKRSDPNNDGDTNYKRTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEL3</accession>
    <name>A0A5B0QEL3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="4">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_004539</name>
        <name evidence="15" type="ORF">PGTUg99_022709</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000206">
        <property type="protein sequence ID" value="KAA1123815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEL3"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000001">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF46">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="13">
        <location>
            <begin position="9"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1111562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1123815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="352" mass="37894" checksum="A4237BF23E7F204C" modified="2019-11-13" version="1">MMASKQGGVKLITGNSHPQLAQAVAQRMGISLCGCKVKKFSDGETSVAIDESVREEDVFIIQTGFNPGAGPGSPSDPNDLLMELLILISACKTASAKRITAVIPCFPYSRMDRKDRSRAPITAKLVANMLQAAGCDHVITIDLHASQIQGFFDIPVDNLWMEPIMAKWIRANVEDWKNSIIVSPDAGGAKRATALADKLDVDFALINRKRARKAGVLRQSMSGPNGPMEHSEQVEEKMEILVGDVRGKNAILLDDMIDTGETLTLAAKELDQAGAKAVYAIVSHGLLSGDAVSKLAHLPVLKLVVTTTIDQRSHISESAGKLETMDISPVLAESIRRTHNGESISMLFSNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEV0</accession>
    <name>A0A5B0QEV0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEV0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022947317" evidence="1">
        <location>
            <begin position="28"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="184" mass="20550" checksum="854F809A9045BC49" modified="2019-11-13" version="1">MKKFSCSPLWLTALIIAPILHLPSTACSTIPKASPVLGQPMLSKRAVIVPPRFVIIPIFGPVSFTEIFNWGCPTPTDNNRVGDVYAKAHPEMAKEVSLDLREILRLEEQSGQVSQTDHARVIIKARADLARFFEAHPELQAQAEDTFQDQVDTLFRSRASADWEAADLDQVASRDWAHFTPQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QF35</accession>
    <name>A0A5B0QF35_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QF35"/>
    <dbReference type="OrthoDB" id="2508052at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022671948" evidence="1">
        <location>
            <begin position="27"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="91" mass="9399" checksum="00CB9770F9101CF6" modified="2019-11-13" version="1">MRSFASMLSTALVLGVFASLTANVSALLCNTGYTATCDGPDQTVYHTPGGSGNFFCKGGSKAACCAVIATCYQCYYDLGNGMDNPKIISSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFV5</accession>
    <name>A0A5B0QFV5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFV5"/>
    <dbReference type="OrthoDB" id="10411618at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="443" mass="49973" checksum="7A5825783C72F429" modified="2019-11-13" version="1">MVIPAFNGTSRQKNLGLAPTHPGPRRNISPPPSENKNLNPPKIQEALLPQSTGKQPLKSPGSRTVVENKTYPPGTKAKSPTLPLENSKLNQRNFTERSHTPESTFGPLPSDLGRTEKLSIAYRPKDGMPFTKEEYARKDLKFLVSSTIQIGKIKNLEQLKLFRIKFEAITHYLIRNGHNSHMDEFANYLWKSLTPKLERDISDPLIWDGHLVLDDNFHIAELPPYDVILEYIYMELGQMDTLQEAPGQPEQDIHQPNQNQHNFINMEECNPEMQSPPTENHSPVPSSNLYCCPLILQNDPSQEHLDREEIEFGSETVCLPEIIPSEISSPCALIEEDFSPVASIALESSPQPLENSQQLGPMDQYESKNLGSESSGTILQELVVPVEDTSDNPLPGLPENCLEIIGEDKNEEDFFYLKELELQMKPVQKIKKSSFQKVIPNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIJ5</accession>
    <name>A0A5B0QIJ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015898</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIJ5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="61" mass="6718" checksum="6AD245BD821B5EB2" modified="2019-11-13" version="1">MTNLITDFHIGGHLIKPSIPHASSTTPPGVVDGIWTPNRMYQMPSGLGPARSIHRMRFAFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIU7</accession>
    <name>A0A5B0QIU7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIU7"/>
    <dbReference type="OrthoDB" id="10347913at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="57" mass="6241" checksum="1B2F5AEAFB8E3F66" modified="2019-11-13" version="1">MPSATFELEVPFHPVSPLPPAQRPPGEAGGHRYRAASAVTLSQLYSCIPDRFRHPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QK54</accession>
    <name>A0A5B0QK54_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QK54"/>
    <dbReference type="OrthoDB" id="2509207at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="401" mass="45208" checksum="AE5A478265D3D66D" modified="2019-11-13" version="1">MSEVAVLRKELSNLQTRLEILEARLTTIPLAPVQISSRRLAKRKDKLVEDKEETSRVSIKPCKTSSSRSKRDAQTLRSRSFKVMRHLINVVDISHDEGRASELKKICINRDTTHHSATSRLPAMTAEESAKLKTTLTLALKAPAVPSNSQNKSHLSATLLRPTTNFRQSVEVKPMKEPLPPSSNLKQIPNTSSETPRLFHPKRTHKADLASSNKVANNKNSQAASELPVMKKLNTNTHSKLEGAVPKTSETLAVTRNLGNKKAEIIECNDLRRGCGRFALLKYDPAVLDLLPALVKDRRFLETKTAIDIENLPQASANLRQVLGIWEWKEGTSSATFHEYKTPELGTTISNKGFRFVGFGWRGDLGQQPPRIEELHEDISQGADTLRWYWFKDLWLLFYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QU89</accession>
    <name>A0A5B0QU89_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QU89"/>
    <dbReference type="OrthoDB" id="614844at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="289" mass="32279" checksum="899E8A3A43F7104D" modified="2019-11-13" version="1">MPGIELPSLGTQDGQEPALSEEELSHLASSLALEASLDPGRDGETRRPAPPSQANPALSQEEQQQQSSDDLIPSGDPCHHNLKLRNVSVDLLQRITARTVLQFILSLLDGEDLAHFVRSPSASLPTTLAYSPTRHSVISSVSKNRDSFARQARSETQLIIAQSLLMTRAQILEEFESDMTDRNPEEDNHQDEAETPKEENEAEGRRTTLEERLNEQNVSRVVEDELKDLIRQKFTDYSHPFIDHQAAFERSISLVQASELISFSAQVLSWNRLFDLLFSSHSGSNPPCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX43</accession>
    <name>A0A5B0QX43_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Transcription elongation factor Spt6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SPT6_1</name>
        <name evidence="14" type="synonym">SPT6_2</name>
        <name evidence="13" type="ORF">PGT21_001400</name>
        <name evidence="14" type="ORF">PGTUg99_007827</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Histone H3-H4 chaperone that plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression. Essential for viability.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the SPT6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000016">
        <property type="protein sequence ID" value="KAA1136903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="995477at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09928">
        <property type="entry name" value="SH2_Cterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09918">
        <property type="entry name" value="SH2_Nterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2740:FF:000002">
        <property type="entry name" value="Transcription elongation factor Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.850:FF:000001">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.140:FF:000007">
        <property type="entry name" value="Transcription elongation factor SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000056">
        <property type="entry name" value="Transcription elongation factor Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000065">
        <property type="entry name" value="Transcription elongation factor SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2740">
        <property type="entry name" value="Spt6, Death-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.850">
        <property type="entry name" value="Spt6, helix-hairpin-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041692">
        <property type="entry name" value="HHH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049540">
        <property type="entry name" value="Spt6-like_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028083">
        <property type="entry name" value="Spt6_acidic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042066">
        <property type="entry name" value="Spt6_death-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032706">
        <property type="entry name" value="Spt6_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028088">
        <property type="entry name" value="Spt6_HTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
        <property type="entry name" value="Spt6_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035018">
        <property type="entry name" value="Spt6_SH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035019">
        <property type="entry name" value="Spt6_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028231">
        <property type="entry name" value="Spt6_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055179">
        <property type="entry name" value="Tex-like_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14635">
        <property type="entry name" value="HHH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14641">
        <property type="entry name" value="HTH_44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
        <property type="entry name" value="SH2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14632">
        <property type="entry name" value="SPT6_acidic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21710">
        <property type="entry name" value="Spt6_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22706">
        <property type="entry name" value="Tex_central_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14639">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036947">
        <property type="entry name" value="Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158832">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="13" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="1160"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1473"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1539"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1562"/>
            <end position="1575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1117254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1136903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1603" mass="181287" checksum="B78D4B7F6C8FB1C1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXZ6</accession>
    <name>A0A5B0QXZ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable alpha/beta-glucosidase agdC</fullName>
            <ecNumber evidence="4">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGT21_032767</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Glucosidase involved in the degradation of cellulosic biomass. Has both alpha- and beta-glucosidase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXZ6"/>
    <dbReference type="OrthoDB" id="5839090at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090599">
        <property type="term" value="F:alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030459">
        <property type="entry name" value="Glyco_hydro_31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF67">
        <property type="entry name" value="ALPHA_BETA-GLUCOSIDASE AGDC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00707">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022755472" description="Probable alpha/beta-glucosidase agdC" evidence="16">
        <location>
            <begin position="23"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="18">
        <location>
            <begin position="110"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="17">
        <location>
            <begin position="263"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="19">
        <location>
            <begin position="665"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1118166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="896" mass="100517" checksum="5161789408963AEA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYG7</accession>
    <name>A0A5B0QYG7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028886</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYG7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="190" mass="21788" checksum="5CCE51F1426CC387" modified="2019-11-13" version="1">MDQVFIGFVPSAIIPGLSKVRKKIRKKQLADISYIPSPINQVQERILVARPDEYIGTNRCFTFILKTRKQGNQSTFKPVGESEIKSLVSVVRLTIDWYIAYEETSFTVLLYYHSAGSPIKRTYTVINVRVTSLALLRMGSMIQKAPIKRWKQRLTDHWKQSTVPRKSSTPEIVVKVRLYNDYGYGSGDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1C4</accession>
    <name>A0A5B0R1C4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBM1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1C4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023082076" description="CBM1 domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7183" checksum="E7970672A05D6ECF" modified="2019-11-13" version="1">MLSTMLSRAFIMVTLLGLANGILGEDCPAGYALGCTAEHMISGQAGTKYCEPWSLHCCPHTPGRAPCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1E1</accession>
    <name>A0A5B0R1E1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1E1"/>
    <dbReference type="OrthoDB" id="10631002at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25356" checksum="CFF75F9CD2F1A8F7" modified="2019-11-13" version="1">MNGQAEREETDDYVLTDGKYRCKSCPLATGARSWARHCRTNAHRLNSERRAEEIVSARTRGEGRNDQRSTGDDEGVRDPGDNGDVQDPDEDDVQDADDEQFWDHIEAIRNLHSDPPVNEDPRTLDEDLDELEAMDTAPKVPSVPGARRDWQDVLEKELQEIGADDDDIMGPVEVPIKRTDVRSNRVNTSRWFPFKGKMVALCEDTRHIGSLRHALTSLGNSSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R221</accession>
    <name>A0A5B0R221_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R221"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="88" mass="10337" checksum="EB36BE0CBE2C9AF2" modified="2019-11-13" version="1">MRNISIYPPLLTGIIRRYSCRLRALEFDISTLRALSRYNMPRSIHHCPGTTIRKVVADLHRNMQCQVAETFDFFLVFIQPHPSFTRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2V1</accession>
    <name>A0A5B0R2V1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_033856</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2V1"/>
    <dbReference type="OrthoDB" id="5327538at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072354">
        <property type="term" value="F:histone H3T3 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024604">
        <property type="entry name" value="GSG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419:SF18">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HASPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12330">
        <property type="entry name" value="Haspin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01331">
        <property type="entry name" value="DUF3635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine/threonine-protein kinase haspin C-terminal" evidence="10">
        <location>
            <begin position="436"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="493"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="589"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="503"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="541"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="589"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1119780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="727" mass="82040" checksum="B7A00E3469B6FED9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R377</accession>
    <name>A0A5B0R377_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R377"/>
    <dbReference type="OrthoDB" id="2495628at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022924403" description="Hydrophobin" evidence="1">
        <location>
            <begin position="21"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14164" checksum="A824473C3C386F9E" modified="2019-11-13" version="1">MQFSNLMIVFLSVALQLVAGKDAPYIFSCDKLTKDGKPVPQAICGIMIPDPKNPVPSQFSLVQAKMKLDSKRGYAKNNLCEDSGRVSLCCFENTATFDKSHTNTTRPLKLVEKQCVAPSPIAKRNTVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4K8</accession>
    <name>A0A5B0R4K8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_037282</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FUN14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4K8"/>
    <dbReference type="OrthoDB" id="163794at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346">
        <property type="entry name" value="FUN14 DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346:SF10">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1120223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="222" mass="25293" checksum="EDC5FD8623B523D4" modified="2019-11-13" version="1">MLTPIPLDHPNTKTHQKLDHQHPKKLPNNSTLDPKTIILKDQDLSLDTPRNHRISTKPVIQLNAALPPQTFDPQPQQPVELEPAEFTSLLSVRKLSFGTLTGTCAGVFIKNGLNFLTFLFGGGFVLLQINWKMWAKQYEYKFWSAKEPPTKSIVTRFLDFLTSDIQYQSTFTVGFFLRLRIGGISYTLDFKIQSMHGIDYSLRNGNSIESTIKLIVDFLQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RBI2</accession>
    <name>A0A5B0RBI2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_035984</name>
        <name evidence="5" type="ORF">PGTUg99_009777</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000212">
        <property type="protein sequence ID" value="KAA1123030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RBI2"/>
    <dbReference type="OrthoDB" id="8026949at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1115396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1123030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="161" mass="17576" checksum="46B96373662EDBE3" modified="2019-11-13" version="1">MDIPHRRILLHAAFWESKHNPNTPIALWIGHIRVAADELLTINELPTDRQIANRLVAGLGSSWSAVKDSIIYTAQEMLLDDNIGALEAQEVSLNGTPSTDFASDSFASSKRFGCSNCGKRGHKSSECRKPKTKAGAVSAVKLGGYDSGSFEDREEVDVVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJ14</accession>
    <name>A0A5B0RJ14_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034593</name>
        <name evidence="4" type="ORF">PGTUg99_018957</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000176">
        <property type="protein sequence ID" value="KAA1125667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJ14"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009141">
        <property type="term" value="P:nucleoside triphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.180:FF:000003">
        <property type="entry name" value="Type I phosphodiesterase/nucleotide pyrophosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1125667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="602" mass="68636" checksum="0350D8D72D809219" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S2Y9</accession>
    <name>A0A5B0S2Y9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012669</name>
        <name evidence="3" type="ORF">PGTUg99_037194</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S2Y9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138248" evidence="1">
        <location>
            <begin position="22"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="70" mass="7323" checksum="72336FADAEC73AEF" modified="2019-11-13" version="1">MKCFSLSAAFLAAVLVSVSSSMQFNAAEKLTAVQDANSAGASEYGGQDSIQAACAECRALGIRCYHFCHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S966</accession>
    <name>A0A5B0S966_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014392</name>
        <name evidence="4" type="ORF">PGTUg99_006304</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1133985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S966"/>
    <dbReference type="OrthoDB" id="3067228at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="127"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1133985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="345" mass="39670" checksum="30E1A1A19484DB44" modified="2019-11-13" version="1">MRGYEGEGGKSKRRRKKPKTLKGQAVSQETERASQQFIESMQSQPNQPNSSQLSREPYFTGPLNNENHKQSVGRITPDPVDPQVESLSQYFQNQHHKHRHVLEEQQWADVYGPMFSSFYDCAAKTANWGDIEKWNSDWKPTCGCIPTRQRPVVLVDILSRKEERIDFCPCQPDQVRLIQMGYIGATPKFPKTAFSILLLQLHHILWKHCTIAMLPFSKALDEFLDINNPLILVPHGEDDLEESVSTCHWRKQLSKAVDAFREMLKREKELVAQVMKLEPLGELADICPKCYGPQVDGKREGEPDYILCMDGNFQHQRHLKASVEHSENIKTPSLFVQPCEVSKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SK16</accession>
    <name>A0A5B0SK16_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_007988</name>
        <name evidence="11" type="ORF">PGTUg99_001855</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SK16"/>
    <dbReference type="OrthoDB" id="2495998at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051615">
        <property type="entry name" value="Transcr_Regulatory_Elem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313:SF81">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="52"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="472"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="472"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1067514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1137709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="495" mass="54505" checksum="08019C9DFDE03C7E" modified="2019-11-13" version="1">MDLNEIDYELGLFLASAPGMVPEASTCQIQSSSAEDLAPKREVKGARRVRNACLQCRERKIRCSRTYPCKGCVSRGDGAACDWQGGFPTGNIPIYQKHAADQRREIERLKDRLTSAKACLGSALQNNPNFKLEVGHHQTRPAFILPTTPREESLSWFSTGSNDQPPGSALSNISSFSANTVDSSQGWSHDEATSPTSATLQTTIWKERNPSRLFEMRHKTTGYRPLSLLREEDELAVQGSRPYTALHPVLRRATSSDKPLAPGKPPIHSGWSSDFLDSIVPRGRIPHEAYEISNSKACQEAVTPEGNSSHHSTSDLQEGMPAVHRIHFPTSLPPNRPNWQYNAPKDGRAVHNHEGQSGNPAKVESQSPITPSRHNLGFSLPYISSLRADETLPGGVRNTCQQHYLPQRQYLGGQDVEPITRPTMLNTRDIPWSSDGHSPNLEQRIQSNVAVGLVNMGEQYSLLPSTFGIAPTTSSSSQLPTYNPNSATNWPPVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLX9</accession>
    <name>A0A5B0LLX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010314</name>
        <name evidence="3" type="ORF">PGTUg99_016364</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000506">
        <property type="protein sequence ID" value="KAA1068101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLX9"/>
    <dbReference type="OrthoDB" id="2499225at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="335" mass="37646" checksum="4562F0B1271F9AF2" modified="2019-11-13" version="1">MFSSSIDDHMFQEVPRNSSQLQCTPQTRPSLSIPNSSAPPPGILDMSFNRRSLDWSELQQFSPHWTLSDQQSNLPPTASRSPGDIHSQLSDWNFPSQPPVPPRLVLWGPFNKTEQSHFHQSAFSSEAYPHHPPSASHPLTPPPHGSKTPKRGRQGCFPSACPPLVTPPMPSKRPRTAEKEAYEFPPPPRYTAYHLSAYPTPMASPSSQNSTFYANHSMAEESLSISPFSSRFESFPQASPADLERSYHQAPTSAHSDFPYAQLSPSSSSTCNYGYYPDSPISPTTPLSSPPLWENSSPFSTSTTRCHQLNFQNPQYPNVWSLEWHQRFNQFRYQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQB1</accession>
    <name>A0A5B0LQB1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQB1"/>
    <dbReference type="OrthoDB" id="10063408at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="117"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="770" mass="89629" checksum="87DA82711BC95A4A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUM9</accession>
    <name>A0A5B0LUM9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_017830</name>
        <name evidence="13" type="ORF">PGTUg99_034893</name>
    </gene>
    <organism evidence="13 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="13 16">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUM9"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF270">
        <property type="entry name" value="FYVE-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DH" evidence="11">
        <location>
            <begin position="335"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="595"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="12">
        <location>
            <begin position="954"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="73"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="148"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="199"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="795"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1108"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1133"/>
            <end position="1149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1068657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1104274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1149" mass="126875" checksum="1BD30F4B911AF760" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2024-05-29" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYD7</accession>
    <name>A0A5B0LYD7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYD7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="58" mass="6556" checksum="52EA280D944B97DC" modified="2019-11-13" version="1">MQNELSELHSLFDWVRPGALGTLRMWEAFVSNPLLQARKSNASVYEMQLGAVGSVKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M152</accession>
    <name>A0A5B0M152_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein CPL1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_031324</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M152"/>
    <dbReference type="OrthoDB" id="2503117at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048661">
        <property type="entry name" value="CPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038955">
        <property type="entry name" value="PriA/CPL1_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35192:SF2">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35192">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21671">
        <property type="entry name" value="CPL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022911474" description="Protein CPL1-like domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Protein CPL1-like" evidence="3">
        <location>
            <begin position="86"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1069678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="172" mass="19223" checksum="AD89552B0E6B977E" modified="2019-11-13" version="1">MNMILTVLTIPILLTFVVSNPHPSFPSASARVHHRNERPSYDQESEESRCPSPLHSCPILIAHHLEVEPNDSILPTPVRKPETTSWECVDLQEELTACGTCDNDCTRIPHVKGVGCENGTCRILSCKSGFSPHFHKDKGGRMRITGCVAKKSHHKFDFFGSLNQRPGARKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1P1</accession>
    <name>A0A5B0M1P1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1P1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="262" mass="28324" checksum="76305A496B3E602C" modified="2019-11-13" version="1">MEQGSNGPYSPSFGGRYATSSEGLVLSSVPCPLRPSETRALGKASSPDHPLLPSFHSSRVIGSVLKCGRWDSHIPILVIGVARSLITDLQTPWDPIGIPSSLPSKSQQTPKKQEIRTASSEQIINQVEIRCKAEARGDTPFTYDYSPAITDLFIWLFVTYKLIWGKSSNPVRLQVTVPASSTGILISQISAINSSSKLESGPNSPNNSVKTILGLRHISGRLLIFVSSSVSPPNIIRSHHPSIHPCRRQDSVLPAVDLGLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2M5</accession>
    <name>A0A5B0M2M5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCT-beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CCT2_2</name>
        <name evidence="10" type="ORF">PGT21_014653</name>
        <name evidence="11" type="ORF">PGTUg99_026896</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2M5"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03336">
        <property type="entry name" value="TCP1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000002">
        <property type="entry name" value="T-complex protein 1 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012716">
        <property type="entry name" value="Chap_CCT_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02341">
        <property type="entry name" value="chap_CCT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1070519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1089888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="523" mass="56388" checksum="743100BFAFBBDC6A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2N4</accession>
    <name>A0A5B0M2N4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015088</name>
        <name evidence="3" type="ORF">PGTUg99_025814</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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        <location>
            <begin position="25"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070529.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1089879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="184" mass="21221" checksum="771354DABFEF1414" modified="2019-11-13" version="1">MQKTQAFVWILLISILLEANTIRAANQLMRQWRLRGDASGRVTSLPIIPNQDDRIIFSSDLETKEWKVENSSSEHSLLCKIRKSGTINWRVVQIIPPNSEIRTGIGMKKSIWVEYSIYHPRPQRNNALNSQERLSGDLVSLRRNKKKLSDHDVSTQLASNNQSLRKMVLSFFMMLLVITPHALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0M5Y8_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1071961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2507845at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="282"/>
        </location>
    </feature>
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        <location>
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            <end position="364"/>
        </location>
    </feature>
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            <end position="610"/>
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            <end position="935"/>
        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="313"/>
        </location>
    </feature>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="452"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <end position="559"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
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        <location>
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            <end position="929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1071961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="935" mass="99621" checksum="61718F51B1EAE8AA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7N4</accession>
    <name>A0A5B0M7N4_PUCGR</name>
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        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08681">
        <property type="entry name" value="C2_fungal_Inn1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
    </dbReference>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037791">
        <property type="entry name" value="C2_fungal_Inn1"/>
    </dbReference>
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        <property type="entry name" value="Ingression_C2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052">
        <property type="entry name" value="CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052:SF3">
        <property type="entry name" value="INGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="769" mass="83056" checksum="7AA044A79C4120B9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8V0</accession>
    <name>A0A5B0M8V0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8V0"/>
    <dbReference type="OrthoDB" id="3142841at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052979">
        <property type="entry name" value="Adenylate-forming_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="223" mass="24827" checksum="AFE4DABA6BCB7EF2" modified="2019-11-13" version="1">MIFIIPLVVGQWITVRNVPVTVQAPSNKASIIRVPGGLTSGLHTRVSRGGLREWHIFGSISEGKHADCHYIVMAVQGGFTRAMNEDQPETLYTKTWKPCGLPYFSRLFHRGVAICTGSGIGAVGSTCIQHDDWFLIWIGADLESTYGTEFMDFIKSKIQPERLLIWDTRGPLGRPDVNVELERVYKQWNAQIALFIGSPALNKSVLRTSRARGIPVFGSIWDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB31</accession>
    <name>A0A5B0MB31_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin carboxyl-terminal hydrolase 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">USP7_8</name>
        <name evidence="3" type="ORF">PGT21_010986</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB31"/>
    <dbReference type="OrthoDB" id="2496844at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="186"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="431" mass="49018" checksum="AD199B3DC5BADC86" modified="2019-11-13" version="1">MLQDSIIRRTGGQFGAQENKKPTLNYEASKSLLPSNFPVERGQFGASMQDPAWEEEKHLSDEENDTMSDKETEIDENDPYVLDLPQDSCATLSERARKTLKQTLFLSKSYFTSSKPFLLRMSQKKKNIPFSIELQTFASSKTVIVTAGGCAKKVLFLVQFHPHDSSDPTFNHLSELIEDLYIMSNHRSNLKNKNKISGRMSGIGFRGGYEKGKSAGSYAVRKDLKPSQLELETNLQNKLPRHNQFIADRFRHFSEEAVAKNKQAMKEFGIPSWSDEAWESYKNDPTPLCSNVIVTSNDFSNKPHCDKDKNIFTYGIFSYIDRSTGTPILPPSSTRGHGIRFPDYDCNINFGTTPGIIEVLWKSNDICHHTIGPPEELKSTKKSTHFGCSFQISHRLVARALKLKCLPEEEKKKRTMTREERSKKKVSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ME36</accession>
    <name>A0A5B0ME36_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_003394</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ME36"/>
    <dbReference type="OrthoDB" id="5348404at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022867405" evidence="6">
        <location>
            <begin position="20"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1074383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="123" mass="14214" checksum="844A2CFA710648AC" modified="2019-11-13" version="1">MLQSSILLLLALSLACVNAYECPEENKEEVDRDGFFHEGFHVGWDMHHVGWAAAGVMASIATIASLANIYLHCKNYNKPLEQRQIVRILLMPAIYSISSFFAYRYYRHYVYFAIIRSRSILHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEN7</accession>
    <name>A0A5B0MEN7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028904</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEN7"/>
    <dbReference type="OrthoDB" id="2517495at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="207" mass="23350" checksum="0F92A0B746306505" modified="2019-11-13" version="1">MQWLVVEINSSALMIDGQLIAGPAVPIIDIEPESEIVPQPSADEDTDELEEPGEWAEPYGIRHMRESSLRALNEINDVLKYIKNRRLLAQNSSALLMNKLKEDSEWLCQFVEERCEGVQITNRLVVAGVQDTGSLTSAEVHDLIGLLVEAAWSGVRRTIDLLKKKKHKIQVDRKCSRESMESFKEKCWSALGMFKEGCGQPSDKQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEY3</accession>
    <name>A0A5B0MEY3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEY3"/>
    <dbReference type="OrthoDB" id="409136at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="311" mass="35278" checksum="80876FBB01D3919D" modified="2019-11-13" version="1">MAQLSTDRKGSSNQDQSDQATKDSNPGSADYSIPASNRHPDPILSRLLLNDQFGKPIKDPKLFFRDCKVVGFFFSAIWKYDRESFQQHVIELCHRNPHRFKCIYISIDSSQQDFEKSTREKPWVNMIWEDGSNMEEDKPAKLESLDFITPIEQELVKQILAGKVDQAQDSRPLSRVGLCSGLEVLFAPTLMVYHLESRTWLDSNVSHGAIASKEKRELALETWEKGERLTLTLMDILVGLRWAILIATVGIIYFFLVRADDAYNIVHLVEWFMAGAAESSHSAPSTPGDSSSPPISNHLHPPKNSHQYVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MH16</accession>
    <name>A0A5B0MH16_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">FAL1_1</name>
        <name evidence="16" type="ORF">PGT21_034978</name>
        <name evidence="17" type="ORF">PGTUg99_005024</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="17 19">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX48/FAL1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000179">
        <property type="protein sequence ID" value="KAA1125326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MH16"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18045">
        <property type="entry name" value="DEADc_EIF4AIII_DDX48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000498">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="15">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="53"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="234"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1075419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1125326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="395" mass="44809" checksum="329EE521BDF1A61E" modified="2019-11-13" version="1">MALNPNQDKLLFETSEEVGVAPTFDSLGLKEDLLRGIYAYNFEKPSAIQQRAILPITKGRDVIAQAQSGTGKTATFSISILQAIDTQVRETQALVLSPTRELATQIQSVVLALGDYMNVQCHACIGGTSIGEDIRKLDHGQHVVSGTPGRVYDMIRRRNLRTRNIKMLVLDEADELLNLGFKDQIYDVYRYLPPQTQVVVLSATLPYDVLEMTTKFMTDPIRILVKRDELTLEGIKQFFVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTRRKVDWLTDKMRDANFTVSSMHGDMPQKERDAIMGEFRNGSSRVLITTDVWARGIDVQQVSLVINYDLPSNRENYIHRIGRSGRFGRKGVAINFVTNEDVRILRDIEQYYSAQIDEMPVNIGEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MI95</accession>
    <name>A0A5B0MI95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004755</name>
        <name evidence="4" type="ORF">PGTUg99_022330</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MI95"/>
    <dbReference type="OrthoDB" id="2496879at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="280" mass="30642" checksum="13C25D8DF3111DDE" modified="2019-11-13" version="1">MNSTKDQPAYFGAPSPQRPLSMLPPKPAQLTQRQKNLVVHLKATEGKTSLVAPAYFLFSCFGKLIFGFGASMAVKGVCTNDTPSIGAGGGLCAFGFICILIVAFGFGAACHRIDKFRGILFKCISILAAILIAVGYAVNQYYVSTHHELMDIRLSFFPLFLGAALLVISISNFFAYTELVRGPTPGLPRERRPLIAMCFFGPGVGTVFGGWITGEKGEWPVAFNAAVLTLAMACLYFGICLFWSDHLLREAEWAEKSVMLEAYRLSTNEITDRLEEKEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIF3</accession>
    <name>A0A5B0MIF3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIF3"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="91" mass="9960" checksum="377F3582A9CF5101" modified="2019-11-13" version="1">MKFLPELAAAVTDAYPVFLGFAMWAAGSFDANYELVTTVKDESSPTGWKDQGNWNSIKKFIPAKGSEKSAKSPSSKPKTKKKAACKRRAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLG5</accession>
    <name>A0A5B0MLG5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLG5"/>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1150" mass="125946" checksum="A9791B69DAA55C31" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLH0</accession>
    <name>A0A5B0MLH0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014893</name>
        <name evidence="3" type="ORF">PGTUg99_025321</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLH0"/>
    <dbReference type="OrthoDB" id="2501121at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="306" mass="34392" checksum="215FB16F9249E814" modified="2019-11-13" version="1">MTTQSSSSSNQEIITDRIVIPLATDERCWFKCRQSNAGRLRGQEPMCSLFCYYSNQLPSLITDPATALKKARTSTSSTSIVPSNHQDKNDVLSDKKEEPEDRWDELIRKYTFSYFEGKYFYFATGKPAVIRHLASMRQLGSVEHDWLVQKFKEEDEAIHEKSKLAGKQIPDKSHRGLVVLAYHKDYESVINIAHSIASGLASVHAHFEKIYGPSYKILSKLPGSMRQLIDPEESKVPSQFTMIHGLFTQPLLSNSATLLNRSFESLSEVLGRLATKYASRFDDEKKDNSNGRFGGKPGPPPSPPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MM27</accession>
    <name>A0A5B0MM27_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome biogenesis protein RLP24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RLP24_2</name>
        <name evidence="7" type="ORF">PGT21_029080</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MM27"/>
    <dbReference type="OrthoDB" id="10262490at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF8">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="TRASH" evidence="6">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1078127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="241" mass="27413" checksum="0DD46AC0A8C77B9D" modified="2019-11-13" version="1">MRIEKCYFCGANVYPGHGTMFVRNDAKMFRFCRSKCHKNFKMKRNPRKVRWTKAFRKAAGKEMAIDSTLEFEKRRNVPVKYDRELVQTTINAIDRVAAIKAKREKAFYAARMAAAVPVVRKSMAVEVVKDRHVLGLKDDELTQKAVEAAESRLAVINAREEEKKASRKTKKTHTEDLEMDAVVEETGESNVDLTQAISKALVSTALATRSEPEKTKMKMKIKNKVTRKSALVPANQSMDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMG9</accession>
    <name>A0A5B0MMG9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025858</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMG9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="12794" checksum="CB32308C8A61B638" modified="2019-11-13" version="1">MDHFADGYKQDMYNACIPFVSPSSQSSSRGWLHKAGLISLSRTSGSMSYNVVHRDRQGAMLPIEFLVSLSTTPFHRFSSPTSFLVAPWFLTAGGPILKPMTEGAQTGLSYLPASILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMR2</accession>
    <name>A0A5B0MMR2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rho GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RHO4_2</name>
        <name evidence="10" type="ORF">PGT21_012124</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMR2"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032506">
        <property type="term" value="P:cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04132">
        <property type="entry name" value="Rho4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000678">
        <property type="entry name" value="Rho GTPase Rho4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1077568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="283" mass="31098" checksum="88CBF4B591B40931" modified="2019-11-13" version="1">MSSYATNHHPNEHNNNSSNTCDYRPEYDTSNPHKSGYQTTQTTASGSSYATYGVYGDVAQSSGSNLAGISSASTANGSVSGTLSYRKPDAKRKLVVTGDGGCGKTCLLIMYSENRFPEAYIPTVFENYVTMVPFPTDSSKFVELALWDTAGQEEYDRLRPLSYPESDVIFICFAIDFPTSLANVTDKWHPEVSHFCEGVPLILVGTKIDLRKDQRTSDLLAAQGTTPITPQQGQAVAKEIGATYMECSAKHNIGVKEVFNAALRLAMRGRKMEKMKRRVCKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPH9</accession>
    <name>A0A5B0MPH9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_021746</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPH9"/>
    <dbReference type="OrthoDB" id="17458at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07722">
        <property type="entry name" value="LACTB2-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000783">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.201, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047921">
        <property type="entry name" value="LACTB2-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041516">
        <property type="entry name" value="LACTB2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050662">
        <property type="entry name" value="Sec-metab_biosynth-thioest"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131:SF0">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17778">
        <property type="entry name" value="WHD_BLACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="84"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1077840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="417" mass="46115" checksum="DCFAC2609C213EDE" modified="2019-11-13" version="1">MMRKTVAFVAGRAPCYRTHRWSKIIFVNRSSISGTRGRRNTSSHPVSAGSMKEEEEAQIPDVSVVGSGITRILGQNPGPYTLQGTNTYLIAGEKASLLIDTGEGKESYVDLLEPVLRSNPPVSDIILTHRHRDHVAGLPSVLKLISSLDPPSGPSPMSLRRMPKVWKFPSDGATYDLDIRQSIIDATDPSHLHPQPGPSDSSEKTPPLKILSEHQEFPLSAHSKLQILHTPGHTEDSISCLLFEDPERRTDSSKKPESLSAIFVGDTILGGSSTIFEDLITYLRSLHKLLRISTTTNRISDSPVRLFPGHGPLIQNGPDLIQRNIDHRLAREKQILDCFRLHPSSQPSSGHSCVLTAEKLIELMYPVGLPKAVYPAALRGVHQHLHKLRAEGLVDRWPSDSTDQLWRLTDNNTNKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPK4</accession>
    <name>A0A5B0MPK4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPK4"/>
    <dbReference type="OrthoDB" id="2503349at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="436" mass="47484" checksum="BF6511324BA69472" modified="2019-11-13" version="1">MHCYSAKSVNPASNPDPAIPQSRNPPPPPPHTISQLTPIETPTTEAPSPSVLSEVSTDTNKEEPSAFNPEVEGLFNQANTLLESVTLAADSALPDDPAQLATSAKEKANLGAALEKTATPSKLDKSIPPSKVTSSPPQSKETSPTLKTTSPKLSNSPAKKKALLTTPKKSPEKTAAKPSALFLPSKPPTRLISTQPSPEKVRKRQAISVVIPMSTSSRRPMPTIADPPADTRVIVQNYPQESKSPTTAPTQLSDLMDIDHPEDQDPNEGADPSMDIEPTKLNAVEDQACRIYRQQYHMYETAKKAKQHADATRALTECQRSYQNISKKLTWQFALSINNGWNPFKEAKSAKLLAKLEGRSQPTASVSQPSGSYSSSSNHRGKRPAETQLNQLGKIAKLDTSWRETMAAARALQQARAQVLEELRKEEEASKRRKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQK9</accession>
    <name>A0A5B0MQK9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Inositol-3-phosphate synthase</fullName>
            <ecNumber evidence="6">5.5.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Myo-inositol 1-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">INO1_3</name>
        <name evidence="20" type="synonym">INO1_1</name>
        <name evidence="19" type="ORF">PGT21_030399</name>
        <name evidence="20" type="ORF">PGTUg99_022922</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">21-0</strain>
            <strain evidence="20 22">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10716"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="5.5.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.5.1.4" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000274">
        <property type="protein sequence ID" value="KAA1113959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQK9"/>
    <dbReference type="OrthoDB" id="2887at2759"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00787"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000334">
        <property type="entry name" value="Inositol-3-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000907">
        <property type="entry name" value="Probable myo-inositol 1-phosphate synthase (MIPS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11510">
        <property type="entry name" value="MYO-INOSITOL-1 PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0398">Inositol biosynthesis</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="18">
        <location>
            <begin position="350"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1078199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1113959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="576" mass="63246" checksum="B6711816C1BFFBF8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUH8</accession>
    <name>A0A5B0MUH8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015706</name>
        <name evidence="2" type="ORF">PGTUg99_033042</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUH8"/>
    <dbReference type="OrthoDB" id="10665668at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1131604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="183" mass="20938" checksum="B2A86D2072C521C1" modified="2019-11-13" version="1">MLSNKILVSFQILHYFSVSANPLLFSNNLVKRAESSFNRISNFASPGRMDLGDSEMEEAEEQSEHMNEYQHLSNQINNMEIGRQKETIKNIIPSACETAVFPGIPGWLSPYLNNIFPKCSQKLRERGIPNDLISHVERHALELLNSEKISDKVGQMKPEKIDAYINHILPHYHQIATNHIVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUS5</accession>
    <name>A0A5B0MUS5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_050289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUS5"/>
    <dbReference type="OrthoDB" id="2496688at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097196">
        <property type="term" value="C:Shu complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28498">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28498:SF1">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="88"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="148" mass="16536" checksum="7CB52C8C615965B3" modified="2019-11-13" version="1">MNRKCPQTMAFSLETGDDDNAFEVIFNRQLEQIDSTELTDQQIHALHVICGPDILLASLELLDHKAVRKLVLKNGQALYEVQGNEAIYHVQLGFKDSCTCRTFIDGVIIKGRQTMCAHLLAVRIGFRLGSIETQEISLENLVTIFSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MV69</accession>
    <name>A0A5B0MV69_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001258</name>
        <name evidence="2" type="ORF">PGTUg99_005762</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1079928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MV69"/>
    <dbReference type="OrthoDB" id="10503793at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="106" mass="11145" checksum="685ABBA537591B5F" modified="2019-11-13" version="1">MLSIMLNGKASSRGMIYILFIGMIITLSINLSSAITCVKSFQPRGDGKVVCDTGIYTHTCSLNNCYVLVPTNPASEPGAIAFEGCNRADGSGAVATVYVQYPPSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWR0</accession>
    <name>A0A5B0MWR0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031488</name>
        <name evidence="3" type="ORF">PGTUg99_033177</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWR0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="77" mass="8149" checksum="EA7563648327EEBD" modified="2019-11-13" version="1">MHLRKKEEVCAGGFPVSEADSSGSSILPGLSRQPATPHLVISRKPPANNPPSTTINQTTSSNPAETEDPLKQKDLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXA9</accession>
    <name>A0A5B0MXA9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXA9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022990026" evidence="1">
        <location>
            <begin position="23"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="122" mass="11157" checksum="53ED55188232F03F" modified="2019-11-13" version="1">MKFTFGLLSVVATILSTETVTAVPTHGLHRGYLGGYGGYGGGYGGAYGAGYGGPYGGYGRGYGGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGGGLGLGFGLGVGVGVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1I1</accession>
    <name>A0A5B0N1I1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_024815</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1I1"/>
    <dbReference type="OrthoDB" id="2502798at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001482">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF85">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G00710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="82"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="515"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="520"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1083091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="560" mass="61529" checksum="71C172440318BAF7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2P1</accession>
    <name>A0A5B0N2P1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017962</name>
        <name evidence="3" type="ORF">PGTUg99_002607</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000225">
        <property type="protein sequence ID" value="KAA1122625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2P1"/>
    <dbReference type="OrthoDB" id="10297802at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25192" checksum="BD00EE1453F71BAF" modified="2019-11-13" version="1">MNVDVKGNHPTEDVKPSVKPLTPAPPQLHSEVNVGKILKAWPNKDCPKFKGTPGEDARDWLVTMEVLLEDRQAHIGVWNVATITDQSISDQLNRLFQSQNETCQEFYERFRDWQTKAKTYGYQYDKKTTFVKKLIRGLATEHDHKHRKLHPVASTSGGGSGKRQSDGDYGWDRKKKSSCLACYNCKKEGHVLAKFPEPKTDCQKAWEAKHAEEKANKEKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3B0</accession>
    <name>A0A5B0N3B0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylglycerol/phosphatidylinositol transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NPC2_1</name>
        <name evidence="10" type="ORF">PGT21_004971</name>
        <name evidence="11" type="ORF">PGTUg99_003294</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the intermembrane transfer of phosphatidylglycerol and phosphatidylinositol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000209">
        <property type="protein sequence ID" value="KAA1123314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3B0"/>
    <dbReference type="OrthoDB" id="6409159at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00917">
        <property type="entry name" value="PG-PI_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.220.10:FF:000002">
        <property type="entry name" value="Phosphatidylglycerol/phosphatidylinositol transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.220.10:FF:000004">
        <property type="entry name" value="Related to phosphatidylglycerol/phosphatidylinositol transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.220.10">
        <property type="entry name" value="Ganglioside GM2 activator"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036846">
        <property type="entry name" value="GM2-AP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033917">
        <property type="entry name" value="ML_PG-PI_TP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF0">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL_PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033473673" description="Phosphatidylglycerol/phosphatidylinositol transfer protein" evidence="8">
        <location>
            <begin position="23"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="9">
        <location>
            <begin position="74"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1083715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1123314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="202" mass="22279" checksum="3AE1770D46E1440C" modified="2019-11-13" version="1">MTNLSTLKIFVSLVFSLALNLAIQPSATSTVQGLGWKKSKDNLFQSDSLSEISRHFMKNLNSDDSPSTQTVLTFDDCGQASDAVTIESFEVDPNPPEPGQKLTIRASGTVHELIKEGAYADVVVKLGAYIKLIQKRFDICEELSKANATLQCPIEPGHYEIVQEVELPKQIPHAKYKVEARAFTQDDEDMACADVVLDFLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5V9</accession>
    <name>A0A5B0N5V9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013530</name>
        <name evidence="2" type="ORF">PGTUg99_025807</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5V9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="84" mass="9563" checksum="CB84FCA782A16A5C" modified="2019-11-13" version="1">MIEDDNEDKLISETDNHDELASNINLEENNMITEEDTDPEENNMFDNEDFDGLFTNAKSDETISKTSAPFNCTKSNMNVADCDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N735</accession>
    <name>A0A5B0N735_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dcp1p-Dcp2p decapping enzyme complex alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CEG1_9</name>
        <name evidence="2" type="ORF">PGT21_023998</name>
        <name evidence="3" type="ORF">PGTUg99_017462</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N735"/>
    <dbReference type="OrthoDB" id="2508855at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="156" mass="17817" checksum="279AF54A1567A336" modified="2019-11-13" version="1">MAGRPTQEDLQALQAQIVEMQNTLAQLQNAAQQSQVVARREWVIRLFLKSPRGLHHEYNPRKTRLAYDGSNLDIWEREINHTLSFVFASHTHFTSGNYGFSNHPLEEQRCISTLFRWTVDHDLLDIVESCGADSPSEILTLLRSICTSSNRNGGYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7C4</accession>
    <name>A0A5B0N7C4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7C4"/>
    <dbReference type="OrthoDB" id="2500577at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF108">
        <property type="entry name" value="PROTEIN CNPPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="2">
        <location>
            <begin position="50"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="272" mass="30338" checksum="88B0A8F2F1787EDD" modified="2019-11-13" version="1">MSSPTSSRDPYYGYGPISVLSTSIVTTLFPPMVTNPDEPYPVTETGKRMPKLVDFIGLCLVPHSMNDAIVHSSLLLLTRLKQRYPSARGNSLKPHRLFLSALMLASKMSMDDTYSNKSWVIVGQNMFSLSEVNRMERELFGFLNSNCYVTKEELKEWCQEWCGQTDEEEDLVDDEELYELQEPHVHHGPSMVEPTGCDSSDSNSPISCSTTTTSSPSPRTPEEGCEDDVDARNRWKSCQHPVSILPSSANAKPTSHAPTSISSIQHILNSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7E4</accession>
    <name>A0A5B0N7E4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032998</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7E4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="108" mass="11967" checksum="6377842924628118" modified="2019-11-13" version="1">MSSSTVYSISDFSLYLMPKRATPMCPPCQSGTLTSVFEDDEDDEIAHTISVLSKANKLIKKILIRTKLPPRVKSSHSNFFSPSRLRSLTLSSQSSRLTTQSLHSSNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7W2</accession>
    <name>A0A5B0N7W2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">VPS17_1</name>
        <name evidence="5" type="ORF">PGT21_021707</name>
        <name evidence="6" type="ORF">PGTUg99_025562</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7W2"/>
    <dbReference type="OrthoDB" id="9976382at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030905">
        <property type="term" value="C:retromer, tubulation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053055">
        <property type="entry name" value="VPS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47433">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47433:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="224"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Sorting nexin/Vps5-like C-terminal" evidence="4">
        <location>
            <begin position="352"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="606"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="487"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="133"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="608"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="621"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1084228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1092391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="688" mass="78689" checksum="5C87A59E257AB75A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9U7</accession>
    <name>A0A5B0N9U7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000059</name>
        <name evidence="1" type="ORF">PGT21_004985</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000058">
        <property type="protein sequence ID" value="KAA1098803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N9U7"/>
    <dbReference type="OrthoDB" id="2511329at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="156" mass="17698" checksum="254A8B7410D86A28" modified="2019-11-13" version="1">MTSGYSFLIAGELMSGANQRANSPNQPLAVFCRLFDSLSAYADMKARPKRLDERFSPGEIMPQFQFPRRSRRFDLRNHTTIEVLQNFTGRSEELRSYRSNHTATTSTRRASEGAEIAYPAAVWGFFRLGMKSDLINLEKAHGDHLRRSSPGLLRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NA83</accession>
    <name>A0A5B0NA83_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_011635</name>
        <name evidence="11" type="ORF">PGTUg99_016475</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1135984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NA83"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033473779" description="RING-type domain-containing protein" evidence="8">
        <location>
            <begin position="17"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="111"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1085573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1135984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="215" mass="23629" checksum="79DC388E543CCC4C" modified="2019-11-13" version="1">MIPLLLLLILVSNTLQAEFKIEVSQLPTTTQSVETAPNQYNVLNHPLSVNEEDVATAPATCPEMVIHINQKEEGSQRASTGDSLHKSIPVDLATKDGSLEKSDQVKECGIMCANEEDDFAPTLDCGHNFHMQCIRPWLLTSEKSTCPDCRKDVSAQLFQDITGQVLKAPQETEMSIVTSNPGISHRPENPWITPCCILFVLIFFGGVLPSLILLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NA95</accession>
    <name>A0A5B0NA95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000109">
        <property type="protein sequence ID" value="KAA1085008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NA95"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="96" mass="10825" checksum="6FFA4BA362E24DB7" modified="2019-11-13" version="1">MRPRTLARSDSIASKDEVTIPADRSVPVQPMGMTARAVKERCSVPLNPLHTRNTQAIKSWGDDHTKRERFATSRRKSASEDSATAPDIHQPQRRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBD8</accession>
    <name>A0A5B0NBD8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBD8"/>
    <dbReference type="OrthoDB" id="677207at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="129"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
    <sequence length="224" mass="25570" checksum="1E4D1BDC9FA053B2" modified="2019-11-13" version="1">MAANEDEEHGNDEQDQDNETNQEATTFWQRIRTVYCEAVPRPIQTFTSLKKRWSNNLQPAINKFRAIVHQVKGFNKSGASVEDQLNRALRLFATDQGTHFKHLRCYNLLVKSPKWNTYCRDNNIKEAGKKTKTINQLAAQDQSWKQDVACAHKEITSKSKQLNDIFNHDSQSINCMSQNGTTSSELAIMTTDLNGVDDEQKEYFKLKRAAILQSLRAQGNSSTN</sequence>
</entry>
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        </lineage>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
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    </evidence>
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                <person name="Sperschneider J."/>
                <person name="Matny O."/>
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                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">21-0</strain>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDN1</accession>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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                <person name="Silverstein K.A.T."/>
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                <person name="Schwessinger B."/>
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                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086664.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="515" mass="57407" checksum="CB97B9C266D2A19B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDP8</accession>
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                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <comment type="caution">
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
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                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGB3"/>
    <dbReference type="OrthoDB" id="2507803at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022753834" evidence="2">
        <location>
            <begin position="22"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="311" mass="29753" checksum="266CF45F92CD2C65" modified="2019-11-13" version="1">MLSSNFVRLACLLSIVGGAISRPQPVPQAESEEGSLSNSLAAHPHPPANTAAPPPPLDSPAPPPAEDDKPPSPPGAEPGQPGAKKKPGKDAKPVGPPRAPDAKPDGPPGANPDGPGMDAGPPPSLDPQAPEPAPGPGAPPPPGPMTPGKNAGLPLPPDAPGPPGKNAGPPGKNAGGPGKNAGGPGKNAGGPGKDAGPPGKDAGPPGKDAGPPGKDAGPPGKEAGPPGKNVGPDGPPPPNAETNPPPPTKDDPANKKPGAKKPDGSRPPPDGGKKKGDDQGTGDRSSASELSGSVLTPGFSLLLAGIVSLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMP8</accession>
    <name>A0A5B0NMP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMP8"/>
    <dbReference type="OrthoDB" id="2507672at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="395" mass="43994" checksum="1CF34856A8F7ECCD" modified="2019-11-13" version="1">MLHVRKVLKPGSANGKPGALMGNMASKIGRLSKSHDHNTSRNLSTHLPTSAREYYSAPQSYPDHRSEYDQPNSSSRFSNSTSAPEYIPAAESRRGSHLEYYFPNSPSRFSTPISAREYIPEAESHPDRYPAYHLPNLRPLSAPSSFSNAVFQAFSRSQMDIPQRAEHRVKQNVLIIQRPCWSRWFKRNPSQESAFYMESSNRESTLHNVEPPDEPHGRISLSRFPDPPSQNSNANFYKSNAISTAGNTPPSEILMCGGGNSRANSLVFFPIMDGPPESPEVSVPSSVYGQSFSTLPTSPSASLSPKSFNPEYHEELISPQSRATVISNSNSRHHRSIQVEISVLPQEGQSLTLGQISLVPQEYQSVTLDHPSTAIKSRIRTSWQPYNHSIVEEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NP03</accession>
    <name>A0A5B0NP03_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021557</name>
        <name evidence="3" type="ORF">PGTUg99_010109</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000178">
        <property type="protein sequence ID" value="KAA1125428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NP03"/>
    <dbReference type="OrthoDB" id="5945798at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF6">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="171"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="469" mass="52525" checksum="AF0550FE3D4D326E" modified="2019-11-13" version="1">MTSLVSRPWLVSAVVKPLSKNSMSLALHLSSNLILNTQIFKMLHFLLALSSLPYILPMLVGCESINHHAIDLIPSSELHCKKWDSPLPSRLNVANSDYDVSPTIPQMEPFKNGFLKSNCSRNPLDIDPEPFCIFLNTKVNQGMGIVIISKKSVFEASLPKLDFSTELPVPSTIKIVKMPEKGGAGAVASQDLKRGQTVGKVRAVALFPHDQSLWGNEFGKRFYRQAIELLPHRTQDVIASLHATGMEETKEEFIANALKQNTFGTHLNPASPIVYSALVLEPAVRFNHDCRPNVGYYIDHDTQSIHMTAFRPILAGEELTISYRASELTRKMRQDSLSANYGFQCSCSHCQMSSEEGKKSDGRVLRLLQLQNIHSTGVEWLSIEEVTELIKICERENLPYSMINANYIAAQVYNAHGRTQEASDFAKKAKRDGLMYVGPMWKDLEEAQILIDSPQKHNSYLNIIVDDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQC5</accession>
    <name>A0A5B0NQC5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQC5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="9304" checksum="FFB5199CA0847977" modified="2019-11-13" version="1">MNTINDETMRYDAINRPVSVSHRFSDTRLSDTDAMHRLSDAMHPYRIAKPMRYRCIASLKRCDACIYRPYRTHRVKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NRX8</accession>
    <name>A0A5B0NRX8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_033432</name>
        <name evidence="3" type="ORF">PGTUg99_034939</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NRX8"/>
    <dbReference type="OrthoDB" id="424465at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000305">
        <property type="entry name" value="Clavaminate synthase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF99">
        <property type="entry name" value="BIFUNCTIONAL PEPTIDASE AND (3S)-LYSYL HYDROXYLASE JMJD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="162"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1091422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="378" mass="42666" checksum="940FC36610EB220B" modified="2019-11-13" version="1">MDQSEESLQKLVSEYQELNGSAIDQVSGRLPTALELSRSIARNRPLVIRSYAGLQRASSSSSSSSSIEDGHHHHHHLEGLEEWTDSKLVQRLADQPITVARTPFGNADSIVDGTYFVEPAYEKLTMADFLAELRGCSGTKSSQDGSTDAANQDRASSGSRGDVVYLQSQDGNLSKELRPLLPNVGTHVPIASQALGTNQPDAVNLWIGDDRSITSLHNDPYENFYLMIEGSKTFTLFPPVEYYCMHEGRYRSSQYHWSESDQKWTIIPKSSEEQIVPWIPIDPLEPDHNTYPRFRFARSMSVTLHQGDLFYLPSLWFHHVQAHRTTPNGLIIACNWWYDMNYEGAHYALFSYLRRQVLALDQSGLPNTIPKDVEFEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTC3</accession>
    <name>A0A5B0NTC3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTC3"/>
    <dbReference type="OrthoDB" id="3061191at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="486" mass="52966" checksum="3606D3E5C01E15D8" modified="2019-11-13" version="1">MSSPISSSVDPATKSSHQAEARTQTRYQSPGDLDVEIANLLPYSVLLTLTHSRVQVMAENLELLTLEAIPTCFFALEKLATTILHSLQFGATSVELSHQELFNMQINPFVPFEDALSMLRGQTFANLNKSPLENRISILVRDVVNTQEGIEELAGTCLPQHFDSLERTLAHIQGIIARPQLIQVLHTGAGKQTHTETASPPIPHPTGELLRTHRASTVSTVPRAQSLVFFSQELNSAIFNTATSTIFKFADVLGGISPHEMELVLSAPTSGAGTYEEKLAKLTALENELIRDIISTMDPTRLVTDAEEFQGRVERHVQSHEPSCDLQSDLMFMRALRLKRQAILENINKNQQVHSTRIAAALSADKLGGGGVPHTSQGTQSVISSQQNFVNPPPDLPQTSKNLHPAEATYAPAKAHSSPPVIQSKVTEAAIIRSQSLSLSRISLPGQSFNPHPTSNVAAPPLASIDPTKRSDPNNDGDTNYKRTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTU3</accession>
    <name>A0A5B0NTU3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030070</name>
        <name evidence="1" type="ORF">PGTUg99_028699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTU3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="69" mass="7604" checksum="2664BA95E105C83D" modified="2019-11-13" version="1">MLFLDGAWNKPASQSNIDSCLEETKFQIATTNLELENCHGNLFPGRLDCLPGPKGPNDNVDLQPYAHFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NY18</accession>
    <name>A0A5B0NY18_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA polymerase II transcription factor B subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TFB1_2</name>
        <name evidence="9" type="ORF">PGT21_011563</name>
        <name evidence="10" type="ORF">PGTUg99_029963</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NY18"/>
    <dbReference type="OrthoDB" id="2504407at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000406">
        <property type="entry name" value="Putative RNA polymerase II transcription factor related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="169"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="232"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="121"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1092698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1093797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="628" mass="70845" checksum="5C62CA0D8957BDC0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZT3</accession>
    <name>A0A5B0NZT3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZT3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="81" mass="9043" checksum="A70A69926E13338D" modified="2019-11-13" version="1">MIASSFSYYASISFNFITGSQESFLFSDKTRNPPAKDVFKRNPANGNGFQNSFLFLDKTRTLAAEDLSTINPARGKGEDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1S5</accession>
    <name>A0A5B0P1S5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007751</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1S5"/>
    <dbReference type="OrthoDB" id="10519594at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="296" mass="34246" checksum="DC0414A5CDAACB85" modified="2019-11-13" version="1">MKGILIHQYVSDSGQEEKLLLLLWKAKSDLFVQAVHLSAEKQPLQDSQTMGFRLGTKLKEKIFKAIGFRKPAVVRLVKVFNKRYNDYISQFPNNPMMGSNEYPLTYEAFEAMPLDHQFWNDGIYYHSKEPWAISADVRTGIIRLQMFVDNPELSRASSSSQGGEFEDVAGEDVELDHIDLLNLGNLPRIKKLQLINKELQVRRHDVGALAQAWSADITWLWSRCQPIENRRNMEQWVSLLERVYQEGKEQKEPNINKEDSNVNEINDMFEDVVLGVQMDDGEEVDDEWLEENEGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3U4</accession>
    <name>A0A5B0P3U4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_004428</name>
        <name evidence="3" type="ORF">PGT21_005138</name>
        <name evidence="5" type="ORF">PGTUg99_006535</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000254">
        <property type="protein sequence ID" value="KAA1118361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3U4"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033845152" description="GH18 domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="2">
        <location>
            <begin position="83"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1112637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1118361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="466" mass="50619" checksum="28401D9E2CA45EEE" modified="2019-11-13" version="1">MVSQFLRVLLISLPALATSQAPPNGHVSVSFSSSSSSAEAHSNSAKVQFSTDLPITNTTMIPDVGSGNSTHNANSNPKKTGGRKLAGYYPVYNAEGQPLSELRYDLYDEIIFFVATTTENFTLGTYPLNQNEWDSLAFDFVKSCKNHSVTPTYAVGGWGGSRYFSALTATSENRTAFADSTISFAKKYGFEGIDFDWEYASIQGIGCNTVNDADVQNQQLLFKEVKSKWPEGKLSTAQSIAGIRDANYGKLPASDVSLMAQVVDEVRIMAYDVHGSFTKTTGPNAPIRATCADPENQLSVETAIEIYLKQGFKPEQLSLGIPGYAKSWTLAKPELTPRIVGNYTSYYYQNYTGIPAGGRYDDQPGLDVCGQKTGYGGSWLVNELVTAGFLNEDENSGGKGYTRYYDECSGEPFLASETTLITYDDTASTVAKVEYANSKNLGGVFFFDTMGPRDKTVKAARTALSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4A4</accession>
    <name>A0A5B0P4A4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4A4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="161" mass="17231" checksum="8623F9E3F1BEB530" modified="2019-11-13" version="1">MTSSYGVASPSGHTPNFGRKQAKRAGPILKHAWPTVKPAWHPPNPRLAHVKAGGGRSKASLDRNQARVPRPPICRPPRRPAAESGPSAELGHLLPIGPGLEIGPAGLGYVPSPAPRRFRMIKPSGHKSNPRWLSAKRDLGLCPDALARPYKFSAMSLASIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7L5</accession>
    <name>A0A5B0P7L5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008502</name>
        <name evidence="4" type="ORF">PGTUg99_030111</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7L5"/>
    <dbReference type="OrthoDB" id="10272563at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366346" evidence="2">
        <location>
            <begin position="24"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1134150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="333" mass="37951" checksum="A52508EBB3391AC5" modified="2019-11-13" version="1">MRPQSRPLLKVLGILHLIYLTSTVARGGLLAPKDHHLKKRCLPLPNCVAEIMGYFFEKAQGVSDQLSVVTQETAQQRWNAELVKERVSSPLVERSYWEHRTFQVCQNAALNELLKESARSRIDQATIIALLSKKLIHPIEKNHRPDRLPVSYPPGLREPPSEASSPRNEDNVWDKNLEENHEIILAENSASDPLPLGFAAVHENIKSMRSECENLSAFNLETSQWSSIILRLTAVTLAHKVFESAQWKVGREMIFDELISLAYLERTSIYHEIVTGEQATQDLLKEEDWWSKVKGGMSDNLSQIIGPETERSDSQDEWWMALKEKLSHKESIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P816</accession>
    <name>A0A5B0P816_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P816"/>
    <dbReference type="OrthoDB" id="8026949at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="215" mass="23489" checksum="7678CA2DA146B4C1" modified="2019-11-13" version="1">MSGYRQVQNVSSLVATIESLDGMAAVFPCWRSRLEDVLGMQNTLDIVKGSLPRPKDEGKEVASYTRPADSKGYNPKESKEDWDALSEMACSTIRLTLTDPLAQRYHKAFWTVRHDPNKPIALWIGCIRVAADDLLSIGELPTNRQITDRLVGGLDSSWSAVRDSVVYTAAEMSLDDAIGALEAHKVSLNGTNQSNLVSAASAKHFACSHCGKRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9H0</accession>
    <name>A0A5B0P9H0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032458</name>
        <name evidence="3" type="ORF">PGT21_033941</name>
        <name evidence="2" type="ORF">PGTUg99_005092</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9H0"/>
    <dbReference type="OrthoDB" id="2508761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="60" mass="6311" checksum="BA25925568ABD8C0" modified="2019-11-13" version="1">MVGPVFAVSRPSRPASRDGRLALLMIRKVSLAAGRLGQGSKPSDQVAGRTAVKLNLQVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA25</accession>
    <name>A0A5B0PA25_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035338</name>
        <name evidence="2" type="ORF">PGTUg99_007900</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA25"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="65" mass="7281" checksum="6CAC560FD6A42CCC" modified="2019-11-13" version="1">MEESAGTPTDASADSYFRVHTADQEIFEPVMKLLYKICSQIDPLSFPPSTAEEIQPSERFKTVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PB53</accession>
    <name>A0A5B0PB53_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PB53"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="268" mass="29376" checksum="9E905CFE859D2C4F" modified="2019-11-13" version="1">MRVPAEGISEHIIPQLLTLIFGRLTFDNLVPSHPSVIDIGSSSSTPSLDFRRSWLDDRFLSYINRFSFSADTQPRISLIDIQRTLSHLFIGGDAVDPSNSTPQATSVCQPNRAAYCLVFHCRIPATALGIPLRSRLTGADGTAVTDLQETSLIDDASPASLWEDHCRPQLSILSLLNLDLITNTTDLFNSVVIATVVNPSTATIARSITAAISKTSGPIIGPLVDQYGFSHIRFWVLTRVWVFRDSAIKIAYPSAYFILGLIQLAYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIG7</accession>
    <name>A0A5B0PIG7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Myosin type-2 heavy chain 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MYO2_1</name>
        <name evidence="14" type="ORF">PGT21_020207</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        </source>
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    <sequence length="1657" mass="187289" checksum="0A48215BA729210E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMX3</accession>
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            <fullName evidence="2">Dilute domain-containing protein</fullName>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Basidiomycota</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
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    </feature>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="359" mass="39124" checksum="68B18CBCE6F15F8D" modified="2019-11-13" version="1">MITDLLSSSTGSHARPSTGIISRRKYLCRSKAMQIKLNVTTIEEWGRANRCRRAWSGNTWSHSTSCSSGRVSLGQAGARSFASSSRSSLVLKTLNPAQLLKVSRDYRFEVDEPKLSEECRQYLVQTQQDWDRRSDPTSLGTRAGRQTLLILGSPGRPRSPYPLRLRRKRFIKTKDDGLAKAIQAIDAAFGSDEDMEAYGSYEPPVAPNVSASCSTPARCCPSVFPRPPSHWWASSELSSLSDDDESLSDDDDGDDQIHPNGNGHHHQESTDDQSSFETPRLKPSLALSSSSSPSSSSISKPTPLPSTNSHASSPSSSPPSVVTPTTTTTTTVNKNPAQSRNPSSHRPPSCPQTYRSMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQX5</accession>
    <name>A0A5B0PQX5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1102980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10673288at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="661"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="738"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="789"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="942"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1022"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1074"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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    <sequence length="1420" mass="150848" checksum="A24B5EDAE9708411" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
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                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
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                <person name="Sperschneider J."/>
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                <person name="Schwessinger B."/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0PY88_PUCGR</name>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </location>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAA1105860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="156" mass="17675" checksum="C9D8A9B61F600BFC" modified="2019-11-13" version="1">MNAGEALRPRDRGMGSAVIPGCCCHPARLLDIDLWLFHEVILIVIWTWSAFPRLIVKRLLYLARGDDMMRFSRPTVLSRDYSTSQAFKANFPKPAQHLPLLTALQQLSYLHTPSYPHSSASHSDWTLVIYLNHCWPSTQSHSLLPAPLFLLPIASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYA8</accession>
    <name>A0A5B0PYA8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYA8"/>
    <dbReference type="OrthoDB" id="10595497at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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            <begin position="15"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="126" mass="13797" checksum="C4875F4477498A44" modified="2019-11-13" version="1">MSISQLDPSLITLTPSSVQLGRSPSKTLSSGPNLSCLTLASDVSGTSMRQPKDPSASPDARGKKKTRSRRTQKEMAIYRAEQEQMKKLKAQQKRKGKQAKPPGRVRHSARASQAGSQTNREPLILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZX1</accession>
    <name>A0A5B0PZX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZX1"/>
    <dbReference type="OrthoDB" id="2498281at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="508" mass="56236" checksum="64A6EB73B383D35C" modified="2019-11-13" version="1">MEPADYSDYLDGLQSLDQVSNWLDDQLQPGGDSLGSERRSESVANTSSNTQRGGIDPEDEADNNNDGEDFKVAIEYKLFVTLKKNKSKHTNTRAGNKRKAATADAPDKYARLVSKGNKLSLTWNASNTSLAVFKEAVLEVLRVLEPNQVFRLAEKQESEKNLCWYAIIPYGGQFIEKNQTVLDNSKIFARFLEVAEAKGEAKVYLVQNDPKTIAERNEALKQLSEQDKENSDDQSAQAEPTAGASFNRQVIENMWLLRATHMPSEQLTGSMELPVMIDPKDSKRFIALSPDRISLWARAMTKAEFKGITAGAKEEGDNAIDVRARTTEDLASVPRTPGSNRSPAGTASASGSTNHINLSPIQMGMGAYPPSMSWPSGNPWGWGPPNPNFYVPYGGMRHEPPPGWQPNPFLAHNSHLAAGTMNTGPVSSPPASDDTVEINDYLSFCHIDHNCESVQKALVDYGITHYQEFENIKPEELEVFGVKKSKARLLVSNTKKYGRTLKKRRLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q587</accession>
    <name>A0A5B0Q587_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S53 activation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004277</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q587"/>
    <dbReference type="OrthoDB" id="409122at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022663154" description="Peptidase S53 activation domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53 activation" evidence="2">
        <location>
            <begin position="42"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="315" mass="35659" checksum="26BC4774C58487BF" modified="2019-11-13" version="1">MKILLLGLTAIISIAGGAKLSLEDASKAALHSSVLFETHRAPARFQKLGQPPLNSHSITLQIGLKNTRFEDSISSLLERMSDPSHPEFRTHLNDEEFNELIQPDDESIGAVQGWLKNHGFQNDQMKWTAHKDWISLERVPLNKVEQMLNTTYHVYRDKGGEHVIRTEKYSLPKYLHRHVDLIQPTTMFGSLHKQRSRVSNISQISNISNKRFLDNLPDICPGPILVTNGCLRQLYKTESYKVKVPERNMIATTGYLGESANLEDAQLFLKTQRADQLGRSFDVILVNGGSNPQELNKKQIEKQLGVEVSSQAFYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5L2</accession>
    <name>A0A5B0Q5L2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_014531</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5L2"/>
    <dbReference type="OrthoDB" id="20729at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051767">
        <property type="entry name" value="Nucleoporin_NUP42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527">
        <property type="entry name" value="NUCLEOPORIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="141"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="275"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="377"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="489"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="283"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="453"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="517"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="534"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1108506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="601" mass="60919" checksum="ED6A29ADF4C7EF31" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q825</accession>
    <name>A0A5B0Q825_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_030368</name>
        <name evidence="7" type="ORF">PGTUg99_020224</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the GSE complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q825"/>
    <dbReference type="OrthoDB" id="26136at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002877">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF2">
        <property type="entry name" value="GH16429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="282"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1106179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1109229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="432" mass="48190" checksum="86411EACD798DB5F" modified="2019-11-13" version="1">MDGSISSLPTQTTPTPLGLPPSESSRPDRPKLLFMGPRSGGKSSIREVVFHKLQPDQTLHLETTRRITQDDIKSFIDFEIWDLPGPLDSDVLTLPFAHVGAVIFVIDAQSSYLSTIPQLASTVIQAYQTNPALHFEIFMHKIDAVTPDYRSEIIEQVKDRLTDELSDRELSIYGVPTIAIELSHRITYKLTTVFDYSIYDALSRVIMKIVPQQAALEHLMNLLAQQSGVENCILFDTWTKLYIATDSSPTEKPLLEMCTEFMDIFTDLSELYGVPQQISPVTRPTSMTNGLGKNAPMVSSRQVPDSSHTKRSLSRGRASVLSPATRSSSVAVDQNKKPSSLAGKRMITSQVKVDTSFGGPRGVGMPGIEQPGRVISYYEINQYLTLVCITHEEIMRTQGTLIEYNVSRVRESIEKIFQITNGTPSKHRRRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA92</accession>
    <name>A0A5B0QA92_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QA92"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="65" mass="7094" checksum="2BB4ACA0C26AAF51" modified="2019-11-13" version="1">MLHCQADWCLPLDELGSYASKLNLSLQPAGTIAANAGNSFEDPGKRYPRLVSGRSCTICPDMYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAS7</accession>
    <name>A0A5B0QAS7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_019087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAS7"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000808">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="285" mass="30037" checksum="3B4FC3944E00EADE" modified="2019-11-13" version="1">MAASPAKVASSKPNPSTDGKPVQVKLVLLGEAAVGKSSVVLRFVQNDFQENKEPTIGAAFLTQKCRLEDKIIKFEIWDTAGQERFHSLAPMYYRNAQAAIVAYDITKSSSLDKAKSWVKELQRQGHANIVIALIGNKLDLVQSDADQSASNGSSSSNADDEPDDATATPDNATSLPDSTAANPNLSSSSKRQVTKEEAEAYAQENNLLFFETSAKTGEGIVEVFTQIAGKIPLEVILSGRGNNTAGGRAGAANRGSGGAGGTNNTEDSRINLANSEEAKKDGCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBF4</accession>
    <name>A0A5B0QBF4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBF4"/>
    <dbReference type="OrthoDB" id="10268467at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022955178" evidence="1">
        <location>
            <begin position="20"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="188" mass="21049" checksum="1AFA8C29CACA2C74" modified="2019-11-13" version="1">MCFTASIIILSTFTILVKTVFPMVSAMSTGTISTADDRLWRPLDIPTCPNPGEQPWHTGNCRSHQVVWHVERGPCEDGCRNMIRDKIGALACKNCYKIKPGSTRQINICMEHCYLKLPIPNMSSEIIAKHSPGCVPQLISKEGKGNCENCNKEMVTKFSYWGCKTEGCSYYVPESCEKLKYCGSCHEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCM2</accession>
    <name>A0A5B0QCM2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCM2"/>
    <dbReference type="OrthoDB" id="26740at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="19"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="297"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="30"/>
            <end position="39"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="69"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="128"/>
            <end position="139"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="235"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="337"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="41142" checksum="E5C474BAD5DF2496" modified="2019-11-13" version="1">MTTFGKQYSKNSVLEPSSLKCLQRLPNGTDRSKTPDKSRWFTTHSHTAHHRGPKRRSRGKRWSREQSRRTKAVNKKTKVNKQRTVKRRLIRKRKPRSNTKHRECDVLSDSNVQSSPSSESTPLNLQEDQDHNTKYEPDSELPRLYRAGWLTVRQTYEPSQNSESTYMGLITNSYRNFMDQRSKDSKRIKPKDRFYTRKAICLQPIIQNHHHHHTNKESNNQDSERFEQPDRESNHHQPGRPAAWFLFTKNNLEKEDWYHILLASSKLNGPDSKATFQKDASLFDSEDMAKLLEGIDQQTRSDPNAMDERNARQTLPIQLSNSRLGELDYRSDHEKTRQSSNTYLSLGYQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCN3</accession>
    <name>A0A5B0QCN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCN3"/>
    <dbReference type="OrthoDB" id="3357813at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="586" mass="64413" checksum="455B3C085C3B402C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEM6</accession>
    <name>A0A5B0QEM6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005161</name>
        <name evidence="3" type="ORF">PGTUg99_012570</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000114">
        <property type="protein sequence ID" value="KAA1130050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEM6"/>
    <dbReference type="OrthoDB" id="2510090at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="437"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="525"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="546" mass="58068" checksum="1078C9FFE778462C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHV0</accession>
    <name>A0A5B0QHV0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000692</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHV0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="151" mass="17254" checksum="FDCB9431C24E25D7" modified="2019-11-13" version="1">MNKPWVKSTSAWWRCPLHYAGRPRHQNSTKRGQYQTCTQTSENADVGDPDKTDQVTKQTAHQIVIAPSPAYSNRFMRINYQMIQKKTLIMRNQPYSTGCWVNSHKRSLTVQSRHIDLKVKAVQLEVGNGDVGSIIEVLNCVGKVQRETKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJL9</accession>
    <name>A0A5B0QJL9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cellulase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_028405</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJL9"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142:SF1">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="7">
        <location>
            <begin position="33"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1113338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="198" mass="22098" checksum="FED407175BA5B59A" modified="2019-11-13" version="1">MRHFHRKLNGGDKKYARRDGKVIGEAEDLPASSLVDFWTRIAQKFGEFPEVRFGIMNEPHDVKLDIWIETLQAVVTAIRKTGATNKIILPADNWSHLQTFATSYNAGMSKIHNPDGSCTGLIFEIHQYFDSDGSGTSAGFPVPHTKELQEVVALLAKDDRQAMITEFGGGHNPDCPSVLADFVEEAHKVSNARDLWLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QML2</accession>
    <name>A0A5B0QML2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QML2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022667624" evidence="1">
        <location>
            <begin position="21"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="11507" checksum="9AB4169736FD6FA2" modified="2019-11-13" version="1">MHSFIPLVFLFAMMVVRLEATTFVKCLNENFPQPVCRLQATSSTPDLLVGMYACFQDSFTCSANKNDSKPWKCCEATNFRPGTVRYDITESLKNCRTPTPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN34</accession>
    <name>A0A5B0QN34_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN34"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="67" mass="7503" checksum="14336ADCECA317FB" modified="2019-11-13" version="1">MPLVLCMQHSCDRSKKSSSNNVLLLPIHAVPPHLLKTVAKSRLDPLIILGLRWLSIDELAELLLYGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNL7</accession>
    <name>A0A5B0QNL7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein RER1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_010943</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RER1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNL7"/>
    <dbReference type="OrthoDB" id="448250at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004932">
        <property type="entry name" value="Rer1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743:SF0">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03248">
        <property type="entry name" value="Rer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="20"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1114484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="41381" checksum="4C1C01CE3C352443" modified="2019-11-13" version="1">MHENVTEVTAIEESGSDCRFQNTAGEKARLKFQTPGRSTLGPKRGKLEAETGDHPRSRGRRNGGWAESKVRLVRLREPRAPFQVNHPLTTHRDTTQHALSQNDGRAEYIEGTATLPEEKPALARNHPQPQSTNLEKEIVKRSKTKQTNRLNLPKMMSADSNLMQDEESNETAFTRKYRELEKAYQYQLDRLTPYTTYRWLTTTGLIFIFMLRILLSQGWYIVTYALGIYLLNLFLSFLQPKFDPSIEQDAAENEVEEGGPGSTSNLMGGQNMDGDEFKPFIRRLPEFKFWHSATRATIFSLVATCFEFTDVPVFWPILLVYFLVLFSITMRRQIAHMRRYKYVPWDYMRKARYTGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ10</accession>
    <name>A0A5B0QQ10_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ10"/>
    <dbReference type="OrthoDB" id="2504565at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="27174" checksum="8F17213E3284735A" modified="2019-11-13" version="1">MYKSRGSSCFFGGTHWPLISELTSDFLLINNSNSLRLSNSSTNRTSNLIDLVNFHQSHQPSRLQSSSLTSSTQSSSYHHRVLSICFSLHLILIVVSKTRCMTDNLNTSAASKPTNTNQASTTQTTDSKPDMDKINAMILKTAIEAIPLLTQDNYSMWSSRMMNFFELQKIKDTFIKEDTENLTGEDELQARTILISKLDPSIHSNVINHENNSNAIMIWKSIVHHFASTQAANQARVWNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTN3</accession>
    <name>A0A5B0QTN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTN3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="105" mass="12403" checksum="9D03A18E1EE08A08" modified="2019-11-13" version="1">MRYRYMAIYRIASHRFTVALHTPSEQIFLAFPKAAYEPLDQPSSFHRTQRLPYRNLNTYLSAHILARLNLSLSPPPHQKPQQNTRTKKTHRKRKIAHIEPTLTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QV06</accession>
    <name>A0A5B0QV06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024127</name>
        <name evidence="3" type="ORF">PGTUg99_035936</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QV06"/>
    <dbReference type="OrthoDB" id="2507436at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="376" mass="41826" checksum="E1ED20C2047B4B72" modified="2019-11-13" version="1">MLSPPINHAEVTEDAPPAAGSPVRLPELGPDNGAEIAAVTQADWLPGVNCAVPSNGGHSAGSNELELDLAFLDQVSTFSNVPLPSPNSEYSVSTDEADSEESGWDGLLADNVAPLALDDMRCQGNQASRAKPKADKGAWWPFRAKEYLISSLIIGHTCTIISRQMFDHVRLMFRLCRVLLPDWTTIRRGKAQLRKIIGMDVVGCRLVLSTPLHYLSLKKTLAMELGNPWVAPHVKFYPEFTAFKGVSRLSQSSKWLTELHSDNRAQMIRHDHRDYYIYELAQLISDRIVVPIFFYESGGEMYAKCVSPDIEVDREVGRIKFKIAKDLPFNSPELEAINAKDFYVEYPSMMHNGVKISDLCGDRLYGKPILSSLCVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVU4</accession>
    <name>A0A5B0QVU4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_005945</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVU4"/>
    <dbReference type="OrthoDB" id="545910at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14279">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.590:FF:000010">
        <property type="entry name" value="GRF zinc finger domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032854">
        <property type="entry name" value="ALKBH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212:SF4">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="26"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="349"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1117396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="585" mass="65619" checksum="73D2FDBA37B5CFD9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWS6</accession>
    <name>A0A5B0QWS6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWS6"/>
    <dbReference type="OrthoDB" id="2507575at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="177" mass="19891" checksum="86C8CA101FABAED7" modified="2019-11-13" version="1">MQTNNIEDEAPTTPPNIPDTYSNPHGPQSIRRESSRQHTPSHRPGFIPTSNDSRRSFIAPVDKTRKSKPAAPCTTLIDCEDLESESTRSNRINLGTQFKHRDGRVAVTEVRQTASHTGKVVQVDTIQDSDEDNAKAEAIKNKERTLLLDKDGFDHPKLYFYPQGQGPKQGFIQVSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYW9</accession>
    <name>A0A5B0QYW9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_030087</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYW9"/>
    <dbReference type="OrthoDB" id="2410195at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="5">
        <location>
            <begin position="81"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="229"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1118035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="353" mass="38939" checksum="2932A66211248DE7" modified="2019-11-13" version="1">MPISEAQQLAEMILQAVKDIEADTAAQIPGGKTTNINLPTVAAEDNLEATPQRRTALRTLKAATHQLLATLMPVGLHVQELHYSFHQTAALDTVVRARIADLIHSIDPDSSKGGVHVAVLAENAGMDPRKLSHILRFLALRNVFCELKPGYWANNRCSFPLRTDSPNTIWNALGHLREEIALPALVELPEVLLDKEGGRALSWDHKKSAFQKYYEPGCDFFDFLAKSKDGYRAERFGKTMIELSRSCGTGDAHYKGYDWKKLGANGTLIDVGGGIGGPAYAIANYLPGWKIVVQDRAEVAKSGKENYQKIGSTANLEFEEVDFLKAQPAHRVQGADAYFLRHILHDWPAKACV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0X3</accession>
    <name>A0A5B0R0X3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0X3"/>
    <dbReference type="OrthoDB" id="2506728at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16616" checksum="28313618EEB1FE4F" modified="2019-11-13" version="1">MSSNSHSNHPLYAFLQSDPSRHQADPEISAARVQGTSPAEDADSEKIGADRGLNKHLISLLTTVQAHSSQLDQMVKHLNSLVRRVESVESIFSDGQEQIHQSVAEFRSDTEKIGESPFIRAIFIYVVWSARKEDFENLHVTFWVNTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R170</accession>
    <name>A0A5B0R170_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000709</name>
        <name evidence="1" type="ORF">PGT21_000792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000112">
        <property type="protein sequence ID" value="KAA1084967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R170"/>
    <dbReference type="OrthoDB" id="3357341at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="93" mass="10983" checksum="2ACF713B11F229B0" modified="2019-11-13" version="1">MCRLSIGFFRRPPTCQFLDFNIRRINNLRGTRDQWRKRVDLFNDYLTGLERQIRAIEAIKLRVTEKSSTSELVSQGHKLFEGLLFLYPSVHAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R6T6</accession>
    <name>A0A5B0R6T6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024662</name>
        <name evidence="3" type="ORF">PGTUg99_024511</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1120979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R6T6"/>
    <dbReference type="OrthoDB" id="2508176at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="79" mass="8776" checksum="E725FC5056F51D29" modified="2019-11-13" version="1">MRVVYDLQSPTCPIGAPSRDTMRNRNLKPPQQAAPKVDTPPTASSPNRRQGMVFNVKEFPKELKDLQSEAGSRKITQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RPQ3</accession>
    <name>A0A5B0RPQ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase RsuA/RluA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_011055</name>
        <name evidence="5" type="ORF">PGTUg99_005522</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000151">
        <property type="protein sequence ID" value="KAA1127760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RPQ3"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="3">
        <location>
            <begin position="173"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="355"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1105532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1127760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="545" mass="61657" checksum="B714890EBBC10710" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RWV5</accession>
    <name>A0A5B0RWV5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016797</name>
        <name evidence="3" type="ORF">PGTUg99_020858</name>
        <name evidence="4" type="ORF">PGTUg99_032504</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000136">
        <property type="protein sequence ID" value="KAA1129625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RWV5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138214" evidence="1">
        <location>
            <begin position="22"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1129625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="91" mass="10187" checksum="5FCCA9D9EB6AD8CD" modified="2019-11-13" version="1">MKLLTIVALTLVTSGISMVNCALMCENCKTYTLIEDPKNVICHRFARKYYKCSNEGCARGMYIQVRGPAPTCRATTKGCVHCQKDNKPKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SKK7</accession>
    <name>A0A5B0SKK7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033732</name>
        <name evidence="3" type="ORF">PGTUg99_032776</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000003">
        <property type="protein sequence ID" value="KAA1138357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SKK7"/>
    <dbReference type="OrthoDB" id="10532531at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366754" evidence="1">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1138357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="57" mass="6609" checksum="BA37CB12D7319506" modified="2019-11-13" version="1">MNRSSSALFVALLFAFFSSSLTTIIRDPHVSAQFETWNYHQTSHKVGQRWQNHPESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SM35</accession>
    <name>A0A5B0SM35_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_034874</name>
        <name evidence="17" type="ORF">PGTUg99_033249</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="17 19">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000001">
        <property type="protein sequence ID" value="KAA1139002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SM35"/>
    <dbReference type="OrthoDB" id="448946at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099128">
        <property type="term" value="C:mitochondrial [2Fe-2S] assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000003">
        <property type="entry name" value="Acyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002148">
        <property type="entry name" value="PRK00982.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="15">
        <location>
            <begin position="75"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1110950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1139002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="153" mass="16682" checksum="E769F1BA06025C2B" modified="2019-11-13" version="1">MALTRYALRSRSIARTALSKINTVPKLAAYSTRVSFQPLSSGSRSTIPQFSPALNRPAINLQSIRFASGGSLSKDDIQRRILEVLGSFEKCDPAKITPTASFTTDLGLDSLDAVEVVMAIEEEFSIEIPDEEADQITTVSEAISYIERTPEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LI68</accession>
    <name>A0A5B0LI68_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_000066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000200">
        <property type="protein sequence ID" value="KAA1063975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LI68"/>
    <dbReference type="OrthoDB" id="2504055at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1063975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="122" mass="13389" checksum="E4ABC2A6008A36C4" modified="2019-11-13" version="1">MVGSSSTQILKFESQLQALLSLKKMIQSVETVQKNVKNVCTLLLVITLVQLIALVWCSYRILGALYFQAGVLRKAASRQINLQYEPKQIEFGFCQPSDPSSLNKIASAPQALSRSGSMHSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJJ1</accession>
    <name>A0A5B0LJJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJJ1"/>
    <dbReference type="OrthoDB" id="10268811at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1064495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="109" mass="11521" checksum="652B0F7A45D76B35" modified="2019-11-13" version="1">MSEWAEDCTTFVPSTYPSGFFTAGQSMLDRPVGSLSYKPAGLGVIETLELDFENGAGLASNCPVNSTGYGIGEVNVVRDECSCESGEKVMALHKSGRLRANPTPWTPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LN37</accession>
    <name>A0A5B0LN37_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_028535</name>
        <name evidence="12" type="ORF">PGTUg99_012580</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the asparaginase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000142">
        <property type="protein sequence ID" value="KAA1128354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LN37"/>
    <dbReference type="OrthoDB" id="542841at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006528">
        <property type="term" value="P:asparagine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08963">
        <property type="entry name" value="L-asparaginase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000003">
        <property type="entry name" value="60 kDa lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.40:FF:000001">
        <property type="entry name" value="L-asparaginase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041725">
        <property type="entry name" value="L-asparaginase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00057">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="9">
        <location>
            <begin position="18"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="10">
        <location>
            <begin position="312"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="583"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="616"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1066327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1128354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="655" mass="71770" checksum="9F54FC3CBAC7F63B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNP5</accession>
    <name>A0A5B0LNP5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007689</name>
        <name evidence="2" type="ORF">PGTUg99_005025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000518">
        <property type="protein sequence ID" value="KAA1063924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNP5"/>
    <dbReference type="OrthoDB" id="252265at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18785">
        <property type="entry name" value="Inv-AAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1063924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="169" mass="18414" checksum="FC756EDCCC88F820" modified="2019-11-13" version="1">MLTTSDEIPLECMRKAIALARLCKPIPTAFCVGCLMTKTGTGEVVSEGYSRELEGNTHAEQCAIMKLSPLDGPSSSMLAGDLDLYTTMEPCSIRLSGNQPCADLILEFNRSHHPHLRIKNIFLGVVEPDDFVNCEGVKKLQDSGLTIIQVVGFKDECLSIARGEDKNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPW5</accession>
    <name>A0A5B0LPW5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPW5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="124" mass="13092" checksum="CA076DF141EA2D6B" modified="2019-11-13" version="1">MVQGWLGLCNSQSPKPISSPQFSTTPHHIKYSKDDNWAGSGSPRAGPSRACSFLGRSGRAAFRKSAAQPEPVYNLGRHLNGLSACGGLPVVVSGLPAGIGRNSDLTKRGRHQGSPLYRPDGQPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LTR4</accession>
    <name>A0A5B0LTR4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005774</name>
        <name evidence="3" type="ORF">PGTUg99_015533</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LTR4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="126" mass="14843" checksum="D1832D70C799F885" modified="2019-11-13" version="1">MVCCWSPVERYLDGLLASRDPRVDKEAAASARQWLEDWVPLYYTASVNIIEATGCRFLWPDPDDEPDTEDGEEDDEEDEGWEDEDDEEEEEEEEEEEDKEEDEGWEEEDEGWEEEDEEEDPDNAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWG4</accession>
    <name>A0A5B0LWG4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWG4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="229" mass="25798" checksum="7BBF02A0360496D6" modified="2019-11-13" version="1">MRWATERHKKLAKLMRLLVRVPDNGSSIPEELERLVGHSHLTNTMVMANRLNAACVIVHSSLIQILSLQLEWHIKLPKVFVKTTSQLEDEPLLRDWNSQIDRIKQAIAYGLISGIPGDFSEALIVTLTGDLPNPRESRPVLNQPDLSANLPDQPNHADSLLNQEQPLHFNQPNPPNVAEELPNIHQPLPVMDEAEGDEIDEEEAYHADMENILSETMRADLEQDTGVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYS7</accession>
    <name>A0A5B0LYS7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Diphthine--ammonia ligase</fullName>
            <ecNumber evidence="2">6.3.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Diphthamide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Diphthamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_028129</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>diphthine-[translation elongation factor 2] + NH4(+) + ATP = diphthamide-[translation elongation factor 2] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10174"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16692"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.14" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYS7"/>
    <dbReference type="OrthoDB" id="686384at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06156">
        <property type="entry name" value="eu_AANH_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000010">
        <property type="entry name" value="Diphthine--ammonia ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000194">
        <property type="entry name" value="Diphthine--ammonia ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1490.10:FF:000001">
        <property type="entry name" value="Diphthine--ammonia ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000012">
        <property type="entry name" value="diphthine--ammonia ligase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Diphthamide synthase" evidence="11">
        <location>
            <begin position="88"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1069561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="680" mass="74477" checksum="2F4F71647AD35382" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZB8</accession>
    <name>A0A5B0LZB8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZB8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022942796" evidence="1">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="91" mass="10315" checksum="DE7302F1B9201A3E" modified="2019-11-13" version="1">MRLSALRLLKLFLYLGLVGKSACFITIEDLMDCSNVQFNIWRGAREDASNALNQAMKTGNNVKELGQNISAGRSTIFKRQEDQEKWTTCIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0M6</accession>
    <name>A0A5B0M0M6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050105</name>
        <name evidence="3" type="ORF">PGTUg99_035576</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0M6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="118" mass="12840" checksum="5480BE763E952DCC" modified="2019-11-13" version="1">MSTLSAPLTRGDCDSDSQLRHRGRHGTSNSSQPSPNLNNHCPICIGLLSSLVLILHRLKDFQVNQCCPDGRARPQTIPVCISRHQPNGNNEVLRVSALTGEPMPPQRRAAPHPTSHAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M259</accession>
    <name>A0A5B0M259_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_050112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M259"/>
    <dbReference type="OrthoDB" id="2266637at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21355" checksum="C170475692711C56" modified="2019-11-13" version="1">MAKRKYSADFKYAAVRAAKQGKTLSKINTLLAGSVSKDSLRQWTNLYNTTQAVVRDPSTYKTLGRPFELEGEDLTFISNMVANKPTVYLDEIQRALIEERGVSVCLKTIAKTLHERLNISKKTIRTVNRRQDEEARAYYLSRISCIPTSYLVFTDESGVSLEVVARKRGWAPVGQRTPRVPQERSTHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M4P6</accession>
    <name>A0A5B0M4P6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_016863</name>
        <name evidence="5" type="ORF">PGT21_020188</name>
        <name evidence="4" type="ORF">PGT21_035074</name>
        <name evidence="2" type="ORF">PGTUg99_007697</name>
        <name evidence="3" type="ORF">PGTUg99_023078</name>
        <name evidence="7" type="ORF">PGTUg99_035407</name>
    </gene>
    <organism evidence="2 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="2 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000479">
        <property type="protein sequence ID" value="KAA1071169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1073044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M4P6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1084752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1094397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1096438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1131994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="59" mass="6437" checksum="4C2ADBC56B8DDE77" modified="2019-11-13" version="1">MHPTSRPKVQAAKDRQRHDGRNGGKTTVNPNPSGRRKGLRRNCGKVTDTSEPTAIMPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5E9</accession>
    <name>A0A5B0M5E9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026791</name>
        <name evidence="2" type="ORF">PGTUg99_005917</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1135172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5E9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1135172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="50" mass="5400" checksum="09504687A9E3CFB4" modified="2019-11-13" version="1">MMEKTFTGGDDQDSNPAGHCMFDLRWHGDMIVEAASSKGDDVDIVLSLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7X6</accession>
    <name>A0A5B0M7X6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009098</name>
        <name evidence="2" type="ORF">PGT21_024165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2508085at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="246" mass="27954" checksum="BA4574F25542EF18" modified="2019-11-13" version="1">MSVPSRKEVTANDAADGLFTVFEENFLLTAVTAIEAADRLFTEVELNELFGVEAAQESFEEVTAIEAEDRLSLFTELEHKELFGAEAAQDSIEEEDRLFTELELKQLFGTEAAQDYIEEAIEAEDRLFTELELKELFGMEAAQYLIQEGYITRGNMEHIHNTLSNKYGFSGPLKIDYIETPEGQVVSSNVLFRPMLQEWMRSDPPFDNVEHYFPDYVAFRSMDHDVDNTGGELAALHSDKDDRLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8J3</accession>
    <name>A0A5B0M8J3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8J3"/>
    <dbReference type="OrthoDB" id="2507845at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="591"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="928"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="608"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="708"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="727"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="928"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="949" mass="102399" checksum="C592DAB2E459239F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAG0</accession>
    <name>A0A5B0MAG0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Homoisocitrate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LYS12_2</name>
        <name evidence="4" type="ORF">PGT21_004698</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAG0"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047046">
        <property type="term" value="F:homoisocitrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009085">
        <property type="term" value="P:lysine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF48">
        <property type="entry name" value="HOMOISOCITRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="3">
        <location>
            <begin position="12"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1073229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="369" mass="39953" checksum="1E9794A3796E5644" modified="2019-11-13" version="1">MSSSGALKKALRIGLIPGDGIGKLVIPAAKRVLQETPGVPKLSFIDLEAGYELFQKTGVSLPKETVERLRSECDSALFGAVSSPSHKVEGYSSPIVRLRKELDLYANIRPIHSISKKLEITVVRENTECLYVKQESISSNDPHAPGKVARAIRVISQRASERIGKMAAEVALKGMAVRSQMTEADRIWKGKPLVTIVHKSNVLSVSDGLFRESVRSVFEAQAHDPRYDDIKVDEGIVDSLIYNLYRTPQRFNVLVCPNLYGDIVSDAGAALVGSLGLVPSVNAGDDFIMGEPVHGSAPDILDRQPPIANPIAAIRSAALMLEYLGFTQPAATIYNAVNHLLLHAKTLTPDLGGKSSTDEVTDAIIKQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAQ2</accession>
    <name>A0A5B0MAQ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_035036</name>
        <name evidence="2" type="ORF">PGTUg99_022700</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1073038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAQ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="65" mass="7242" checksum="A2A61816A7D7BDD3" modified="2019-11-13" version="1">MVVPALVIKQRKSKRVGKLFGQRALSVPAHRGEFFHSKDLVPRRDRHTCPPSSYVEGIPKGDSCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFE4</accession>
    <name>A0A5B0MFE4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">GPI12_3</name>
        <name evidence="5" type="ORF">PGT21_022943</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFE4"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1074848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="402" mass="45841" checksum="EA7A9FD77C4DAAF2" modified="2019-11-13" version="1">MSASQSKNRTNQEEEDIPPISPLLEPSYQYPSRDNQIREFDYGDREDVLAEAEELDSTNSHYPPSSKRSANNNNHHHNRTTTKTIINALSLGLRKHRTKFRLLVVFLTLWPLLCWTGIFLLFSDHPALFPSSLRGSKSTLFVVAHPDDECLFFAPSILATVQRAKSHGALLVMSSGNHYGQGGLRRKELLGSCKQLGIREDRCDVLDISQIQDDPIIWWPVDRIGKLVNEHIERWMIDSIVTFDDYGVSGHINHRAVSASVTELAISLYKKSVAPNHSSTKSPKLYRVKSVFVLRKYIGLFDLPLSFIRLIPSIIFGPSQQINSALQSLTYDPKTEQTTQESERQRSAVTTTFEKGLLISGLSGYRSARNAFWSHQSQMVWDRHLYMILSQFMYFNTIERIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHC2</accession>
    <name>A0A5B0MHC2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHC2"/>
    <dbReference type="OrthoDB" id="2516525at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="943"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="955"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1098" mass="124569" checksum="88F327E0E1109C14" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJ87</accession>
    <name>A0A5B0MJ87_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_86</name>
        <name evidence="2" type="ORF">PGT21_026736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1077005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="151"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="1310"/>
            <end position="1333"/>
        </location>
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            <end position="176"/>
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    </feature>
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    </feature>
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            <end position="397"/>
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            <end position="434"/>
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            <end position="457"/>
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            <begin position="588"/>
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            <begin position="638"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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    </feature>
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        <location>
            <begin position="1313"/>
            <end position="1333"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1333" mass="145835" checksum="2DEA611A2C21B061" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKR5</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="3" type="ORF">PGTUg99_019003</name>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <begin position="102"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLB4</accession>
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                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">21-0</strain>
        </source>
    </reference>
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        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1076600.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="261" mass="29885" checksum="8FA78A1730130D3A" modified="2019-11-13" version="1">MSNLSLYDLSAQLNVVGDSSSRAEAKELYKTTLGSPFNLDHCWGILKDTPKWQATQRENEARTKKTTKSETAAPSTDASSSVVAVSSPRVVDIEDNDSEPSRSVLGNGRVEGQKAAKQKRADEALIEKIVAMQKDLVNISRERLSAMNSAKDDAIMSKDLLLMDDESRAYYQRKHRLIMARELEQEEKEKKEKEKKEKEKKEKEKKELEEKERREKEALEADDEEEEAEDEEEEAEKEDDEANGEEEDMEDNEEEEAYDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP42</accession>
    <name>A0A5B0MP42_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
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        <location>
            <begin position="1"/>
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    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1077799.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="162" mass="17199" checksum="1898B6C92B4817D6" modified="2019-11-13" version="1">MGPKPHQDGAAGPAPPKSALAAVGSSPRTAPPGSFPATRPHFHLPSRMRPTAEVTRSNAIRRPSSGPPNRKDSEVNGSLALGLTPTSPPPPIPLTQMPPMKTSTLPLAPLPPVLPVLPFPLPPRRARVPHHRNAMIILAPQDLPQELRDIQLDVSITQDDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MR31</accession>
    <name>A0A5B0MR31_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 12">13 kDa ribonucleoprotein-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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        <name evidence="16" type="synonym">SNU13_1</name>
        <name evidence="16" type="ORF">PGT21_008033</name>
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        <name evidence="15" type="ORF">PGTUg99_023889</name>
    </gene>
    <organism evidence="14 18">
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
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        </lineage>
    </organism>
    <reference evidence="14 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 19">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Common component of the spliceosome and rRNA processing machinery. In association with the spliceosomal U4/U6.U5 tri-snRNP particle, required for splicing of pre-mRNA. In association with box C/D snoRNPs, required for processing of pre-ribosomal RNA (rRNA) and site-specific 2'-O-methylation of substrate RNAs. Essential for the accumulation and stability of U4 snRNA, U6 snRNA, and box C/D snoRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 12">Component of the U3 snoRNP particle. Binds to the C'/D and B/C motifs in U3 snoRNA. Component of the 25S U4/U6.U5 tri-snRNP particle, a subcomplex of the spliceosome. Binds to the 5' stem-loop of U4 snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
            <location evidence="1 12">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000439">
        <property type="protein sequence ID" value="KAA1082151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MR31"/>
    <dbReference type="SMR" id="A0A5B0MR31"/>
    <dbReference type="OrthoDB" id="1924699at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21104">
        <property type="entry name" value="SNU13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000002">
        <property type="entry name" value="NHP2-like protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="13">
        <location>
            <begin position="20"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1079385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1082151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1082601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1133504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="127" mass="13967" checksum="90A63557247321D0" modified="2019-11-13" version="1">MSTATNSKAFPLADANLTNQILDLVQQASHYKQLRKGANEATKTLNRGICEFIVLTADTEPIEILLHLPLLCEDKNVPYVFVPSKTALGRACGVTRSVIACSVTTNEAKELQKQIETVKSQIERLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRT4</accession>
    <name>A0A5B0MRT4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRT4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="84" mass="9579" checksum="699D1ACD84BAF863" modified="2019-11-13" version="1">MLRYKSFKASKARPFTSTSMVCRRVTKRPDDGRFSNAEDFKFSFSTTFRASYGGFESNLTDIGGFKGWQCFLGLFPCCRVLICC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUD2</accession>
    <name>A0A5B0MUD2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SWD3_3</name>
        <name evidence="7" type="synonym">SWD3_2</name>
        <name evidence="6" type="ORF">PGT21_015273</name>
        <name evidence="7" type="ORF">PGTUg99_032470</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUD2"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035097">
        <property type="term" value="C:histone methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000228">
        <property type="entry name" value="COMPASS-like H3K4 histone methylase component WDR5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="5">
        <location>
            <begin position="78"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="79"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="206"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="248"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="293"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="369"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1080631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1131591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="440" mass="48043" checksum="6B6B0D754CE5C76D" modified="2019-11-13" version="1">MSEADPPNNVLLENNATTTDGVLQGSNHEEESNREDHQDTNQLTKESLKNEPEDAEKEAEEDRKLRGNQEAYARVTHTLKGHSRPISSLSFAPDGTKLSSSSADGVILIWNVILPEKPSSDRGGVVGHQVEFMSKLVDGASEDEENDRPREGINDLAWSPDSEYLVSGSDDHAIHVWAPSSQSLPSNPSSSSSSSSSSTTRPIRKLLGHSHCVYCVKFNPIGTLLISGSFDETVKVWDFLAGKLLRTLPGHSEVVSCLDFSRDGSVIVSGSFDGLIRMWDTTSGQCLKTMVVAQETNAPVTFITFTPNSRYLITCSLDSTVRIWDYRSKEGTVMKSYTGHTNIKYSIPARVISRVTTTNKLDPGNPIGDRDVVLMGSEDGSLWIWDLQSRELVLRKASHQDSIIGISVHPTNPSIFATAGLDKDPSIKLVSLSCPPSSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0S2</accession>
    <name>A0A5B0N0S2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1081730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0S2"/>
    <dbReference type="Proteomes" id="UP000324748">
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="66" mass="6643" checksum="BB39F984460A9D82" modified="2019-11-13" version="1">MERANRNSATPSSIGLSRKRSLGHGPILSIDAMSKVVTKCLTSPGASTPSEYGNGNLARAAGGSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3U8</accession>
    <name>A0A5B0N3U8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014989</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3U8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="165" mass="18152" checksum="05DCB1A831F5EE37" modified="2019-11-13" version="1">MERRPELGPMAEVWVTRPRPCCASPASSTECAVACRVSSLERILMEPGDETETHHTTERPTSTFPEPGSNRPMDELMRAIGFGDFLGLTCLSPSLVYALPRKPANGTTALYKGVLPPGGEVSRHPIAVVRQTWFCKAGFHKDLSRLKESDQRNAFLAPSTIPLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5L4</accession>
    <name>A0A5B0N5L4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008461</name>
        <name evidence="2" type="ORF">PGTUg99_034057</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1083458.1"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5L4"/>
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        </location>
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        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="62" mass="6875" checksum="90E4FD9A544E8644" modified="2019-11-13" version="1">MDDESQIAEKRRIRAEKKAERKQQGVDKRTPAPLAHAKPHARCGLACAPARQRKLACGAARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5S1</accession>
    <name>A0A5B0N5S1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5S1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="591"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="699"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="735"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="746"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="805" mass="80111" checksum="BC3DD1804EE3DB80" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9D8</accession>
    <name>A0A5B0N9D8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022906</name>
        <name evidence="3" type="ORF">PGTUg99_006386</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N9D8"/>
    <dbReference type="OrthoDB" id="2508525at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="202" mass="23523" checksum="9CDA77C2438B4D39" modified="2019-11-13" version="1">MNCSPRSQFENYTQKEYQIEAHYEWLYEIELKVIEIVNQWEPLVYEDENEYSTISSDSKFFRPMTEAIQWYKAKELQFKWNEQASAKGIPLSVVPNLREMWAARRKELNRDPVTRPWNESPPRSSSPEQSTPSKPKDSDGSSGGSNSKGKSASLCLTVLFTWIIDPATPHMYPRFTPIDACPLPERWKENDEYNPLSSHRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9U8</accession>
    <name>A0A5B0N9U8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N9U8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036280">
        <property type="entry name" value="Multihaem_cyt_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48695">
        <property type="entry name" value="Multiheme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022688579" description="RING-type domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="17294" checksum="283E0DBAF486D39F" modified="2019-11-13" version="1">MRLIAPMILVTLSSLSMILAAPAEGPALVSGLSKHSTPDCIGTLSLEMRDEPCRTPGCGETLWTVVHRCSECDEVRSDPFESCPKCHVPAVDRTWHGPGCSATTAQISVGTGGSQCTMCQGRVVQWKMRCLTCTRINEKMTACNELCIRCSGRPDRCCSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBH4</accession>
    <name>A0A5B0NBH4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tyrosine/serine/threonine protein phosphatase pps1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PPS1_1</name>
        <name evidence="6" type="ORF">PGT21_007429</name>
        <name evidence="7" type="ORF">PGTUg99_011209</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000107">
        <property type="protein sequence ID" value="KAA1130613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBH4"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14516">
        <property type="entry name" value="DSP_fungal_PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000095">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.9, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053239">
        <property type="entry name" value="Dual_spec_PTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047949">
        <property type="entry name" value="PPS1_DSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="575"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="732"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="821"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="55"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="226"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="260"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1086665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1130613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="947" mass="103996" checksum="2AC79F9A18D573E2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NG80</accession>
    <name>A0A5B0NG80_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component Sec6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021384</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NG80"/>
    <dbReference type="OrthoDB" id="2497674at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022770920" description="Exocyst complex component Sec6" evidence="2">
        <location>
            <begin position="22"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="51693" checksum="7FE5E692FAE7A9AC" modified="2019-11-13" version="1">MRYSCLLRATLIIHSLQMGSFQAPGPCEEDLDDVAQDSARSVPEKISNTAISSGQGLPTSSSAGKTSDSLKRSSQDLLASGSHPSSLEGFQASTRSKQPRIKTDLERRTEDCYKKTLRNFEIWSSSVSRLNLPDDKEARVKLKMEEVKNAISNLFQKRICELRAQPYQFQATESVLSGDLAASITSRWEAIWLKSIAEIDKTWDFPELMNNNPSESIQFIRKGIDLMMDFFIDLQQLEVVTKTCLSDFLNKEDGSQIVSSYVHNRFHKNRLDVCKLYMNFNLKLSLLEGPATQRMVPLLKTLEEDSWKRIEFDFLKDQLETYLNDPVKNSHTDSTEPSMDIFIHYFLALVSPENINFSAETVEDFMEDILYYLKTAINTEIAKLLPIETQSLIETKLAREMIAFIIHHHKDQIANSIMKNKQGTRLYQDMRAIEEAVGLLSSVYHSIYVRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIE6</accession>
    <name>A0A5B0NIE6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003681</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIE6"/>
    <dbReference type="OrthoDB" id="2496748at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48159:SF1">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GIANT PROTEIN ANTIGEN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48159">
        <property type="entry name" value="MULE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="229" mass="25877" checksum="BA1697A0C0E11B21" modified="2019-11-13" version="1">MAQSTINPTSFTYPLVQDIYDVVPRPHTQEIPARVGVSSAPNALRLVQNVGSINPTFPTHPFLFTIPGSSKSAAEEFVKAMTATVRWFLQRGTPTASEKLNKLQGSGRRPEAFFKLEYQCPCSGYCKGTPNSRKKNHISARCGCKARFSVSHHIQSNTLRVIWHWQHNHDLNSHQQMIVTRPPLVVDQWLKDRVDSGLKWREIYDLTQVDDVLDGQFKPQIATNLPFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NK63</accession>
    <name>A0A5B0NK63_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_44</name>
        <name evidence="2" type="ORF">PGT21_000838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000097">
        <property type="protein sequence ID" value="KAA1088580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NK63"/>
    <dbReference type="OrthoDB" id="2509730at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="822" mass="88153" checksum="6E20FA9D44EB3D1C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NK94</accession>
    <name>A0A5B0NK94_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NK94"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="104" mass="11873" checksum="55480577647551E0" modified="2019-11-13" version="1">MVNRKRHMYLHPGCNIFKVRRQTARPANPTWDRRSYSNTPTTQGNTAHHNHQTATTHPAYHLRHLLVGKKLPSNRQQISPHLTATFKHDPVPPDSKLPAFPSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NP81</accession>
    <name>A0A5B0NP81_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-activating enzyme E1 1</fullName>
            <ecNumber evidence="4">6.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_008632</name>
        <name evidence="13" type="ORF">PGTUg99_024361</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="13 16">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="4"/>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NP81"/>
    <dbReference type="OrthoDB" id="10252231at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004839">
        <property type="term" value="F:ubiquitin activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01491">
        <property type="entry name" value="Ube1_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01490">
        <property type="entry name" value="Ube1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12550:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000015">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.180:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.60:FF:000002">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.60">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032420">
        <property type="entry name" value="E1_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032418">
        <property type="entry name" value="E1_FCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038252">
        <property type="entry name" value="UBA_E1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018075">
        <property type="entry name" value="UBQ-activ_enz_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018074">
        <property type="entry name" value="UBQ-activ_enz_E1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01408">
        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16191">
        <property type="entry name" value="E1_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16190">
        <property type="entry name" value="E1_FCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09358">
        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00536">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="12">
        <location>
            <begin position="886"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1074144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1090643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1029" mass="115275" checksum="60AD9AE41F08DD27" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NV39</accession>
    <name>A0A5B0NV39_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000082">
        <property type="protein sequence ID" value="KAA1091950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NV39"/>
    <dbReference type="OrthoDB" id="2505542at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="212" mass="23773" checksum="5FA978F7427340D7" modified="2019-11-13" version="1">MTTRSNPEPLLPLTNPEAILKEGRRQARLEKALKQKTHATGTNIGDLPALPPSPTMSEAPESLNNGKPITPLSMDEYLKGLMKLQHQSIDQANIDRSAALESLKYERELRQADADRIARLEEAVLKMSIKPDPGTTSNQVEMGRIDLQRFRLSDGPVFSGPFQDVERFITWIRSVQIFFATKGVKHEDNKIQVIGSLIRETNMIAFYASGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWU6</accession>
    <name>A0A5B0NWU6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025939</name>
        <name evidence="2" type="ORF">PGTUg99_007823</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000042">
        <property type="protein sequence ID" value="KAA1135015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWU6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1093126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1135015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="70" mass="7911" checksum="611B879C6EAAAF71" modified="2019-11-13" version="1">MAQSVVEEQQEESLHHNLRNILAKLNINTVEEFFGLDKKPKSSQATHSLTQNNYNLRLCTLLQKSGDGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYD4</accession>
    <name>A0A5B0NYD4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alpha-1,3-glucan synthase</fullName>
            <ecNumber evidence="2">2.4.1.183</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">AGS1_7</name>
        <name evidence="11" type="ORF">PGT21_016319</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[(1-&gt;3)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-alpha-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19749"/>
            <dbReference type="Rhea" id="RHEA-COMP:11150"/>
            <dbReference type="Rhea" id="RHEA-COMP:11151"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28100"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.183"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.183" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2496276at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047657">
        <property type="term" value="F:alpha-1,3-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070600">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000052">
        <property type="entry name" value="Alpha-1,3-glucan synthase Ags2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000157">
        <property type="entry name" value="Alpha-1,3-glucan synthase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000114">
        <property type="entry name" value="Cell wall alpha-1,3-glucan synthase ags1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000162">
        <property type="entry name" value="Cell wall alpha-1,3-glucan synthase mok12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058659">
        <property type="entry name" value="Mok11-13/Ags1-like_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058656">
        <property type="entry name" value="Mok11-13/Ags1-like_GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058657">
        <property type="entry name" value="Mok11-13/Ags1-like_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058658">
        <property type="entry name" value="Mok11-13/Ags1-like_Ig_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058655">
        <property type="entry name" value="Mok11-14/Ags1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058654">
        <property type="entry name" value="Mok11-14/Ags1-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47182:SF2">
        <property type="entry name" value="CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47182">
        <property type="entry name" value="CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26127">
        <property type="entry name" value="12TM_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26122">
        <property type="entry name" value="CBM_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26108">
        <property type="entry name" value="GH_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26114">
        <property type="entry name" value="Ig_2_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26111">
        <property type="entry name" value="Ig_Mok13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022861062" description="alpha-1,3-glucan synthase" evidence="9">
        <location>
            <begin position="29"/>
            <end position="2373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1081"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1943"/>
            <end position="1966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1978"/>
            <end position="1997"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="2009"/>
            <end position="2027"/>
        </location>
    </feature>
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</entry>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
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                <person name="Schwessinger B."/>
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        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1Y1</accession>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
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                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
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                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
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            <end position="25"/>
        </location>
    </feature>
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        <location>
            <begin position="26"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="972" mass="111944" checksum="58A2F13E108E6DFF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2N9</accession>
    <name>A0A5B0P2N9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PITH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028953</name>
        <name evidence="4" type="ORF">PGT21_029962</name>
        <name evidence="5" type="ORF">PGTUg99_032474</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PITHD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2N9"/>
    <dbReference type="OMA" id="FANCSDI"/>
    <dbReference type="OrthoDB" id="2635at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045099">
        <property type="entry name" value="PITH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175">
        <property type="entry name" value="AD039 HT014 THIOREDOXIN FAMILY TRP26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175:SF1">
        <property type="entry name" value="PITH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PITH" evidence="2">
        <location>
            <begin position="28"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1108971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1121571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="239" mass="26263" checksum="A00CAC273B179517" modified="2019-11-13" version="1">MPVNNCEGDLTGVDFEQLDPSTSADVFSRAAGGSNSYSLYPYIDQSKSYCLNVDQPPSSSTTGRGGHTGIGAIIRPIHTRNELSPEESLVTHQDTGPEMIVHVVFNQPVRARTIVINVAKGDQSPRLARVWTNRSNAISFDQVDDLKPDQEWELDVELETAVEYPTRVTRFQSVSSLSFEFRDPSAGQRSRIYFLGVLGDVKQLKRDPSSQLTVGAENSADATLDSIREQSGGNQTTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5F4</accession>
    <name>A0A5B0P5F4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5F4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="294" mass="32670" checksum="B78A93326B1FEE71" modified="2019-11-13" version="1">MRPSPPSTDPSPGTYHSYPMSTPPTTNTGVGMNATEPRAPRFQPPPYNLNPPRFPHPVFHPHPPVFYPHRPTFPPFGQGGGARGQGFMQGPGGGRGGGRGRGWIPPAQLRIRGQANRFYHPNGPAAGVPFGHGHAPVDIDQFLNQHPIPPAVQRRPTPIPVPSSPSVPAANIARSPSNLSSIRSGDSFHTAEDFLAPQVRVASPTPAGMYDPDDYHLLARHQIESLLDRRAAPERFDRYEFTPQHFDNFYQSATSALVHHWTRHPAANANERQDRVRLFQFITRRYRTQTRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5Z9</accession>
    <name>A0A5B0P5Z9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019804</name>
        <name evidence="2" type="ORF">PGTUg99_032797</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5Z9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1131894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="132" mass="15150" checksum="A38C7ABA96F4A676" modified="2019-11-13" version="1">MEKLRLMDKFAYAESQLVSNTSKILNYAIKKFDVKLKSYQLYSKAVGEDESPEYFSPASLVLDFGFNKSENPNFNFLFEQVFPSESDHIKIGSSQEILGDSMDKKRKNIIEVDDSFPKDKKGKCGQPINIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7W1</accession>
    <name>A0A5B0P7W1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Type 2A phosphatase activator TIP41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7W1"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="41997" checksum="D1C317972E4958A3" modified="2019-11-13" version="1">MSTTSSSITSPASHIPQHTVTQLPLGNDGVSTADGKRNIQLEGWLVETCKMPILNSKEIKHFGEKLKVPLPEICFGNNYLSIKHPRSGFHYQWNTLDALLPLNPIEGEEQDYIKVAYADHWSQARAVEFSLRGALSDQSHTSQNPPIDPLLLCVISSSIFPPWWLCLAFFGELSCSKESDSVAKVVNPFDWTYTTTYCGTNHSSEIEFNSPSDPRAHGGIPLAVLSRPDPILFFDEVTLFEDELHDNGMASLQVKVRVMPTCIFALSRFFLRVDGVVFRLFDVRFYVDLQTNQIVREIKAKEMKYDILKSRLPAENPHDLSPLTDPNYVSQALQMLKPEDFIQAPSSKEDKPKKWRDMLGAHLDLAAITFPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCM9</accession>
    <name>A0A5B0PCM9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-1,4-glucan branching enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GLC3_9</name>
        <name evidence="5" type="synonym">GLC3_5</name>
        <name evidence="4" type="ORF">PGT21_012843</name>
        <name evidence="5" type="ORF">PGTUg99_017012</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCM9"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 13 N-terminal" evidence="3">
        <location>
            <begin position="54"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1097599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1134022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="130" mass="14694" checksum="6D4276AC6CA9B6DB" modified="2019-11-13" version="1">MMNNSEKPQLVIDVPWLEPFNQTIEARIQKFKEWQAKIDKTEGGLDPFSKGYEKFGLIAQADRSILYCKWAPGAQNASLIGDFNNWDREANPMKKDQFGVWECWVPPKGSQPGIAHGSKVYTGKSTSSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCQ6</accession>
    <name>A0A5B0PCQ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCQ6"/>
    <dbReference type="OrthoDB" id="2508467at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="349" mass="39596" checksum="74A1BEB0060129EC" modified="2019-11-13" version="1">MLRGEATPEPTDIDMRDDDNFGTQLGANPPDASHQADHRRDIDPESESDLSELPDDPLEFLRILQDTVFSRENTMDFARRIFASEGIATGHFTTVIYLETLRRLQATPTAGLIAPIDLAAANFTFGNHIRGFLRDRIRQILTTHNVDAYTQNINSQGRAIERTPLLILRAHVDAQDPAFWGDFLPAGYPEDQSAQLTVMRFMRVLLKHERGQLRNLAYGRRSIEGSVPPLMDLIVIIDRAMASRGRPRPVGAIRQAYNSTTKIRIAFLRLAMAENLVSPQPLNSMTIWEIIDSRLEHVSRQRIAYQRTFSEMVLIKDRELFGSLPFKDIPPETVRLPTDEDVESSPLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PF95</accession>
    <name>A0A5B0PF95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019469</name>
        <name evidence="1" type="ORF">PGTUg99_030303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PF95"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1095417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="71" mass="8089" checksum="45402B78F604E87C" modified="2019-11-13" version="1">MWAITTAIGTQHAPTYPRPSGVEFDGHWPKLDPDQILPQAGFPREVGLLRILFKMLFSGLRISLREDHFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFB7</accession>
    <name>A0A5B0PFB7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Chitin deacetylase 3</fullName>
            <ecNumber evidence="13">3.5.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGT21_009023</name>
        <name evidence="21" type="ORF">PGTUg99_018330</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
            <strain evidence="21 23">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Hydrolyzes the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin to form chitosan and acetate. Chitosan is required to anchor melanin to the cell wall, for maintenance of cell wall integrity, and for proper cytokinesis. Chitosan offers an advantage during infection as it is less readily detected than chitin by host immunosurveillance mechanisms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + n H2O = chitosan + n acetate</text>
            <dbReference type="Rhea" id="RHEA:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9597"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57704"/>
            <dbReference type="EC" id="3.5.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:10465"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polysaccharide deacetylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.41" evidence="13"/>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000343">
        <property type="protein sequence ID" value="KAA1099098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFB7"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004099">
        <property type="term" value="F:chitin deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.370:FF:000009">
        <property type="entry name" value="Chitin deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050248">
        <property type="entry name" value="Polysacc_deacetylase_ArnD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587:SF135">
        <property type="entry name" value="CHITIN DEACETYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="19">
        <location>
            <begin position="242"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1096223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1099098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="452" mass="51375" checksum="C346912C909EE263" modified="2019-11-13" version="1">MSFKGFRTGKSIKSEAEEPSEELSLLKDSLSNPGRRRSYESAEDGEIEEGGHSSFLPTTTSTHRRHQFKFKRTAKKSPTILILTCLIILSVTLIISLYLGLIPSFEAEHPSHQINWNRPPPRNRQWSNYYGLLRPKIGSKEWKMLYPPSKPAGPRPLESWIKRYDYLRSISSSNQLDSPLSTTWTNPQAKLSMQTGLAEYPEGTIFNSDPNGICSYRRASCLRGETEDRGIGLQDIWLGKNHTWSINFDDGPLPPSTSLYKFLDQQDETATHFWVGSNVRDYPELALQAWKRGDHLAVHTWSHAHLTTLSDFEILGELGWTIQIIHDLTGLVPLYYRPPYGDVDNRVRALAKHVFGLTTTLWNCDSSDWSLNQTFALGDFLDPPMNGFGVQESVGMINGHINQPDKSVGKIILEHELSFESVEAFKLTFPNLKRNSWSTCNLADCLDLPWYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFN8</accession>
    <name>A0A5B0PFN8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MICOS complex subunit MIC10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_009416</name>
        <name evidence="13" type="ORF">PGTUg99_003072</name>
        <name evidence="12" type="ORF">PGTUg99_006906</name>
    </gene>
    <organism evidence="12 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 15">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Mitochondrion inner membrane</location>
            <topology evidence="2 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the MICOS complex subunit Mic10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000348">
        <property type="protein sequence ID" value="KAA1098639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1121943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFN8"/>
    <dbReference type="OrthoDB" id="1916310at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007512">
        <property type="entry name" value="Mic10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304:SF0">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04418">
        <property type="entry name" value="DUF543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="83"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1076502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1098639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1121943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="113" mass="11905" checksum="E9FD2B962361ECD5" modified="2019-11-13" version="1">MTSQRLSSSENILSEKTDVCIANAIVKTGMGFGAGVIVSVIAFKRRAFPVWIGIGSGLGAAYSDCQRTFNPVHLPGYRFLDSPPASSSAQNSASCPLPSPPKDASLKSSQRML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGL7</accession>
    <name>A0A5B0PGL7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Galactose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="4 15">2.7.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">GAL7_1</name>
        <name evidence="18" type="ORF">PGT21_035695</name>
        <name evidence="19" type="ORF">PGTUg99_014787</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
            <strain evidence="19 21">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="4 15"/>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGL7"/>
    <dbReference type="OrthoDB" id="418412at2759"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00608">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000002">
        <property type="entry name" value="Galactose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019779">
        <property type="entry name" value="GalP_UDPtransf1_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943:SF1">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00117">
        <property type="entry name" value="GAL_P_UDP_TRANSF_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10 15" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="8 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase N-terminal" evidence="16">
        <location>
            <begin position="19"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase C-terminal" evidence="17">
        <location>
            <begin position="232"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="12">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="44"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="93"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="182"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="348"/>
            <end position="349"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="353"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1098482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1100321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="382" mass="43734" checksum="7BA70851E39CBC67" modified="2019-11-13" version="1">MTSWAWPTTSEYHTHTMQTFDPTQHPHRRFNPLTHQWVICSPHRNNRPWQGQIEPNEDTRLPSYDPTCYLCPNNTRASGQVTEKYTSTYFFENDFAAVNDVEVSQKSDSDPLRMALHRTEPARGKCYVICYNPRHDLTMAELTPDEILPIIKAWIELYSKIKREQPFVKYIQIFENKGAMMGCSNPHPHGQVWALSYIPTIPTTVLKSQEDFASSAIDVPDSARLQDGRPSLLLSYAHSELHPEPSARVLHRTEFFLAVVPFWATWPFEVLVLPHSHHMASLLDLPPGADLDLAMILSHVTRAYNNLFRCSFPYSMAIFQAPPHSEASVAQLHISFCPPLLRSATIRKFLVGFEMFGEAQRDLTPELAAEKLRAAALQPYYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGR2</accession>
    <name>A0A5B0PGR2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031253</name>
        <name evidence="2" type="ORF">PGTUg99_016353</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGR2"/>
    <dbReference type="OrthoDB" id="2500561at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1100186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="102" mass="11495" checksum="81416BEBAA3A19B9" modified="2019-11-13" version="1">MRLLSSGSDGSKIFKRAHWHNTSLLRISDALNRECADGNEDLDHQAVLELLHAKAKLIVPKTLGLELQPNKLEKSSSTHDPFLKKLMDQYGDEFVGHDWSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGS7</accession>
    <name>A0A5B0PGS7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGS7"/>
    <dbReference type="OrthoDB" id="10681454at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="391" mass="45446" checksum="0FA31B4D073EB147" modified="2019-11-13" version="1">MGAATGERNPGSSSRFNQGIEHNDPDFTKTYSDSKDLTVILPVEHGSRPQKLIKLMGVLLKPQGDVTLEQEENTAHSNQPCKPNTLYENSGCLSEYKQDQQPIKASSNPGTTPIAAIPPTLEITKGVTSNDHFLTQRYYKNESESEGLKFDETIFILSPTSPRDNGDISKILKLIRLHLQADKLIMTEKQFIQFCMVLYFNHDTNGDADKKHTITNEERYYQKMSMLDKLLQNHDIWYQHWKKIANIHFEIFPENLGPKMRTMIVTYLLYVEMISMIVPVETMGYSVGAEVRRAFNLVENIYRPRKFTKELRETELYKKTHKIKKSVTNGRANSNVYIGLWALLEIWMQTYRPELFNSIIKEFYWEATKRFFNKIFFHSIENLTKQLENAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIK2</accession>
    <name>A0A5B0PIK2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ubiquitin-conjugating enzyme E2 2</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">UBC2_1</name>
        <name evidence="21" type="ORF">PGT21_018698</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIK2"/>
    <dbReference type="OMA" id="DHKSQYI"/>
    <dbReference type="OrthoDB" id="9984419at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000007">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0749">Sporulation</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="20">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="18">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1099729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="166" mass="18308" checksum="008D431C9EE4CB55" modified="2019-11-13" version="1">MSTASRRRLIRDFKRLSSDPPGGISGAPCPDNIMIWNAVIFGPADTPFEDGTFRLILTFDESYPNKPPTVKFLSKMFHPNVYANGELCLDILQNRWSPTYDVAAILTSVQSLLHDPNPNSPANAEAANLYRDNMKDYIKKVRATVEASWIDEGTIPGTANASSTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKS3</accession>
    <name>A0A5B0PKS3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018190</name>
        <name evidence="4" type="ORF">PGTUg99_036142</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1136594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKS3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137783" evidence="2">
        <location>
            <begin position="22"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1136594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="801" mass="93103" checksum="8E59120EF30C3215" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PL01</accession>
    <name>A0A5B0PL01_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101472.1"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PL01"/>
    <dbReference type="OrthoDB" id="2500006at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="497" mass="54523" checksum="7F52496A9938FF2D" modified="2019-11-13" version="1">MDCSFISAVQPPLVRNRQAHHLARATTQFHHCRSHSESGACLVLPTQILLPMDRRFVSAFVRSLRLVGRLSYYSISSDYSPTPSFDEDGDSFTSFGSIQTPPRLRAMIRSIFDGTLNSPIRSFPDSDSYYAASPREGVSDYVPSPIVTEGLHHGETFGDHRDRLLAALAPSTPDRAPAIDSSASDEEYELDDCALSYFNPSLPCTNPNANLDLLAILVPTPRTDQDHPTIVAGSPNSTSPGYVPNAHIDVGENVGLFNPNDDSVSHVDPRLQLLSEDNNQSDIDSVFSLLDPYEDPTDTTYVPPKKNKQSYAGRKRSSGSLSPKPKPAFVRPRDSKGRFVSLRSKNLGKLKATSVDDQLPRSSSSSTSLPQAVVDSGHPHLTETALNTFLNTVLTSPDEAFPLSFHLPDPFAAHFSLAGPSVLGTNPFIPPTPFIHMRPDDPRLQFRTSFVDLLRAIVPFLSNFTPSPDDHESFKLLNHAVVVSATEYMSQYLSCTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLS2</accession>
    <name>A0A5B0PLS2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLS2"/>
    <dbReference type="OrthoDB" id="2507647at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="593" mass="64079" checksum="0514849110415042" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PN13</accession>
    <name>A0A5B0PN13_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PN13"/>
    <dbReference type="OrthoDB" id="2506958at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="422" mass="48524" checksum="43EF6A672F37B25E" modified="2019-11-13" version="1">MTEPTNNHSEETKKLSEKEELRRQGDLVIQGFKHLADTTNSSLQKDYQRERSITITSVKTKVSVDQPELKKGLLVQLQTSLLPQLQQHVITISQLTNPSDLRKEPASSFKRMLEIQSDLTTILDQISHATDIICSEPLYLSHPSVRRNDQYLNELKPYKLQGLHHRLTTFFLQELYNVFDSSYQTIQQLEFSTKKYNNRIDLTDARDTMIEAVDECLECIELALDWLKGSEFDNVQEDWRYEKCGINETLQKVLSLIDPTTSLSKKKRRLKEPLSRPVVKLAKSVVPVIKLCRLFFNKLSARGMNRRRLPPFTTMCSYQLEIIGALAGHTGAELGGILHVLQEANTTDNEDTRYALTRAVGALETQFQTPFLLILLHFVPIIPDTDGHPVQDYYKKWFAVWNTQFNLAIINLLDAIKEYEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PPB9</accession>
    <name>A0A5B0PPB9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020846</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PPB9"/>
    <dbReference type="OrthoDB" id="3363836at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="371"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="429" mass="46607" checksum="E3AE0F92BA47CA50" modified="2019-11-13" version="1">MSQPHHDLSRRQNQAQQQTFGCQTYHSPTPNQVVEAFSTATLEWDPHCFDSAKNIDIYLQSLDVAQPIHVWKNVVASSAKLSTQFIPDWWNSTQSVSLQIVITPSDLPLWAGLGPGPIFFAHFNGSIPAGAPTSAHEGKQLGGPSIEYITDLFHRSHHALSGGKLGAAIFLPLLAVAIAISSYIFISRRRSGQASKRWSEYVDKRMSVMGSQTWDSPMAGSDPLPSVRGIPGSRVASMYRHSASLPQRDSMIHAPNARLSTFSLNRPVSVRPPTLPGHHSRNTSLAGSAARVSFVDRQSGISFPRPGSEFHPNPNEPPVAPFRPAHQATSSMSRSVSNLRFELDAKPTTDSPSSNKSKPLKVDTEVLSDRASSAGSSDKQSASGMRRLDGASTLSPDEALRQYTLFRGRDEERASTPSNAARADRGSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PU11</accession>
    <name>A0A5B0PU11_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Dyp-type peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_050353</name>
        <name evidence="14" type="ORF">PGTUg99_021563</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DyP-type peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000143">
        <property type="protein sequence ID" value="KAA1128286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PU11"/>
    <dbReference type="OrthoDB" id="3207336at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049509">
        <property type="entry name" value="DyP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048328">
        <property type="entry name" value="Dyp_perox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006314">
        <property type="entry name" value="Dyp_peroxidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01413">
        <property type="entry name" value="Dyp_perox_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521:SF4">
        <property type="entry name" value="DEFERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521">
        <property type="entry name" value="DEFERROCHELATASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21105">
        <property type="entry name" value="DyP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20628">
        <property type="entry name" value="Dyp_perox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51404">
        <property type="entry name" value="DYP_PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137841" description="Dyp-type peroxidase" evidence="10">
        <location>
            <begin position="28"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="DyP dimeric alpha+beta barrel" evidence="12">
        <location>
            <begin position="38"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Dyp-type peroxidase C-terminal" evidence="11">
        <location>
            <begin position="325"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="463"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1104038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1128286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="57000" checksum="861FC443133FF799" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PU81</accession>
    <name>A0A5B0PU81_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_014507</name>
        <name evidence="3" type="ORF">PGTUg99_034263</name>
        <name evidence="2" type="ORF">PGTUg99_036700</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000472">
        <property type="protein sequence ID" value="KAA1075668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PU81"/>
    <dbReference type="OrthoDB" id="2496464at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="417" mass="48521" checksum="7BCBC915969F4732" modified="2019-11-13" version="1">MDGTLAPRNGSMNSDGKSEEENIELTLKILSIVRQRISSHSKDDEDDNRANLVYWLELRNTVPYELRQTILPRLHERIQSATMMLNEFDPTTINPDWWYRRFLIRLMDIAELVEQINSSILSIWTTCGPEQEAPHELPEVEHLTRSRCTRTEFLVQCLIKYDLDDLFGSFEDLFNGLRPTTSSGSLPTASQDSGQIIEGINRRMPPVVDRINSITQWLQEPLVNIAKADWTNMADMFEHGLFYSTYRTKFASEASFLQAFTTFLKFARLLVTKLPQSAPNQPLFFFGPLMDIEDVRLKILMYYSDKIGIWMSTMGFKIQNPHQPIDFREVATVLIRVIGGYSRFLIILERYWDSLLESDAPQVDPNLIVNARQWLNSWTPLFTLAARKVMQATGRLHFHQFHFELREAEHDDFLMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUN0</accession>
    <name>A0A5B0PUN0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GCN3_1</name>
        <name evidence="11" type="ORF">PGT21_024111</name>
        <name evidence="10" type="ORF">PGTUg99_025076</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUN0"/>
    <dbReference type="OrthoDB" id="10249309at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1070">
        <property type="entry name" value="Translation initiation factor eIF-2B, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042528">
        <property type="entry name" value="elF-2B_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1068477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1104452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="374" mass="40958" checksum="5D1414A22493356D" modified="2019-11-13" version="1">MQERFWRSPSRARASFIMPDTLNVTSSPRTHGNIEFDIVEYYHRILEKDENLAVPIAAVQCLSECVAKSNASTMQELIGTLYVAIDLLKQATSNLISLTAGCDLFLRFLMTHQSPDLLVSFVDHKHSLVNQALKYVAESRSNCVEKVASSAARFIEDGSTIMVHGYSRVVLHTLLRAYSPSEKPRKRFQVFVTESRPSGLGLKTHAILEQFGIPCVVVLDSAVAYAMAKVDLVLVGAEAVLEDGGLINYIGCYQMAIAAKAHHKPFYALTESYKFLRLFPLSQYDVPTKQPTLQFPSSKATKSTGVSENIPNLPGSLVHGHSSISAKLTLDQIAKNNPSLDYTTPDLITLVISDSGILTPPGVAEVLLSIFGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUY6</accession>
    <name>A0A5B0PUY6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Fructose-1,6-bisphosphatase</fullName>
            <ecNumber evidence="6">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">D-fructose-1,6-bisphosphate 1-phosphohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Fructose-1,6-bisphosphatase, cytosolic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">FBP1_1</name>
        <name evidence="19" type="ORF">PGT21_025190</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="12">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the FBPase class 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUY6"/>
    <dbReference type="OrthoDB" id="10256725at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000001">
        <property type="entry name" value="Fructose-1,6-bisphosphatase class 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000008">
        <property type="entry name" value="Fructose-1,6-bisphosphatase, cytosolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020548">
        <property type="entry name" value="Fructose_bisphosphatase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006778">
        <property type="entry name" value="PRK09293.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006779">
        <property type="entry name" value="PRK09293.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556:SF1">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00124">
        <property type="entry name" value="FBPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class I N-terminal" evidence="17">
        <location>
            <begin position="11"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class 1 C-terminal" evidence="18">
        <location>
            <begin position="204"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF18913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1104500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="333" mass="36466" checksum="D00D2C68DC5035F7" modified="2019-11-13" version="1">MSSEEMSTEIITLTRHVLNSQARTPQASGDLTILLTAIQTTCKFIATNVRRARLLNLIGAAGITNVQGEEQKKLDVLSNDIMINSLRACGKVALMVSEENDELIMVEDPRQKRKYCVVFDPLDGSSNIDAGVNIGTIFGIYRVPEGEEAGLKHVLKPGKEMVAAGYCMYGSSANLVISTGQGVNGYTLDNGIGEFILTHPDIKVPKRGKIYSFNEGNTMFFHEPVVKYLESIKFPANGKPYSARYIGSMVADVHRTLLYGGIFGYPADKKSTSGKLRILYEGFPMAFLTEQAGGTATTGTERVLDVIPTDIHQRIPIFLGSTDDVEDCKSFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PV78</accession>
    <name>A0A5B0PV78_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028519</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PV78"/>
    <dbReference type="OrthoDB" id="2498445at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="405" mass="44119" checksum="2D7221917B0FC3BF" modified="2019-11-13" version="1">MGQETGHPEATIITTPTTSSSMAIQSPSVAIQSAPQTFTPASASSVNTVQQIFSTIAPAPQPTTVPQLIATAIAKLGEPVNSSPMLPVSSSKLVLTPGVVITTIHTQKMGASHSMSTLDANILPGDQPHMAFASPLSPMILIAIGAAAFCFSLLMAISIWSCFKRRSQHRNSTLSLPHTTHRDSSSPDLQKMDLPSPTNSGYTIDTTPHSKFCEQFDYRLPVLSPTHDPHMHKRYHNDIKASYRIPPAWGATQLDRLPSCPDHLTFVPKVLLTGNLLPPKPAVTPNAMPPYAMPLRFNHHDRVMIRSPPEPRSCKMSPAEASQELQKLIEQVDFRIYEDLDLDESFSESISFSNPELFSGLTGDSLPYGGRPSATNSVAAHPPLPLPRYNYRKVTRTRPSPTLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1H1</accession>
    <name>A0A5B0Q1H1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034210</name>
        <name evidence="3" type="ORF">PGTUg99_026234</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000308">
        <property type="protein sequence ID" value="KAA1107035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1H1"/>
    <dbReference type="OrthoDB" id="10288266at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137930" evidence="1">
        <location>
            <begin position="26"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="88" mass="9570" checksum="550F21FAA1EAB2DA" modified="2019-11-13" version="1">MYTLRVLSAVMLVLSLVCIDMVASARSPKCNMVKDKNQKDAPFLVCESILDKYYNAGQTCQTGEKSSCCTSQVDQTGQVPQSCTPIQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q215</accession>
    <name>A0A5B0Q215_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004818</name>
        <name evidence="4" type="ORF">PGTUg99_014511</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000010">
        <property type="protein sequence ID" value="KAA1137264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q215"/>
    <dbReference type="OrthoDB" id="2506814at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="157"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="383" mass="43624" checksum="12CFF22620B6E1BF" modified="2019-11-13" version="1">MPNNRFNSRSSSQSNTRNQTVFTGSSRQTRTSRIALQQEAAAEARRAASIRLSERLGLSGWLGPVNVPGEMILDEETTESEDTDDEPEQNEGNESPDSLMHDDYPNAHDDDNGIGAHSEYFRLRCYAERRDRISRQWTELNNQVVAAYLLCQKTTSNWTCPSDEFTLPPNTCTCPSNHVTERSVDLLDILYRRPGQKLLFCKCIPDVVRLIHYGYFACSAECPRTAFSIQLVQYHHQLWQASAVSTSAFIQSLSSFLDSRSNTPMFNRGSDRKRRQLRVPFTHCIDLYSRILTAQKKLIEDGLQLTPVDKWANKCPRCFGSSEDEIKDDPSEPDFMIAMDGNFQQRHYAYASKDNPREDQYPPSFLPPSKIAVDMQAITSSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2F7</accession>
    <name>A0A5B0Q2F7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010753</name>
        <name evidence="3" type="ORF">PGTUg99_035931</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2F7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137923" description="Lipoprotein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="50" mass="5335" checksum="206FD4F6E75DD5F3" modified="2019-11-13" version="1">MQSAKFSPVLTTILLLCAAAFVALFGCNGDKPYCVQLTGPSKEKRTLKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2T9</accession>
    <name>A0A5B0Q2T9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2T9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="106" mass="11347" checksum="B9A35D92A0351B1F" modified="2019-11-13" version="1">MYCIRQRADLYGAGRQLSPWATDLDGAGSRLKVEELISSGNHRALSSVCTTGDTSSPEKTKTKKNRASMCSLPRKPGLPRMTSSSVIIDVSSIVVQSHSGSSLSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q360</accession>
    <name>A0A5B0Q360_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014792</name>
        <name evidence="2" type="ORF">PGTUg99_032922</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q360"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1124730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="81" mass="8661" checksum="3635B8868FC7E2D0" modified="2019-11-13" version="1">MTWLISMTLSLQSARGKFHEALSEIDASSCVGQSSGQHRPAPACGGPRCNKIGNIKLQISRGETCLACVVAYSSHFKAPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q369</accession>
    <name>A0A5B0Q369_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018884</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q369"/>
    <dbReference type="OrthoDB" id="2495999at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="193" mass="21777" checksum="9AB3C6643CEE820C" modified="2019-11-13" version="1">MRTEHHHTTTTTTFFHQQLAQFYDIRTQQDTHTLRMTSIASSLLRSYRIIQQGLLSAVTRNRTFNPQQVSRGLVAKHHPVVLQTPPSTILSGFFRPLGSLPIAATGTSALPFSAKPYYSGFHSFAHQRTFQPSPFRTFTSEPRMSLQRELDGGKSTRMAHDHAPRPVNVPLVDEIVHDHVAARFAFPAPSVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5D7</accession>
    <name>A0A5B0Q5D7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006653</name>
        <name evidence="3" type="ORF">PGTUg99_035949</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000002">
        <property type="protein sequence ID" value="KAA1138765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5D7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474406" evidence="1">
        <location>
            <begin position="20"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1138765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="61" mass="6716" checksum="37330AE464FAC8B6" modified="2019-11-13" version="1">MKFIVPTFLLMVFQMHVLATPAPHDELPVGLEDADTRAQASISKRNYNPPGLRCCTGCPIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5S1</accession>
    <name>A0A5B0Q5S1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012258</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5S1"/>
    <dbReference type="OMA" id="QFTQVFR"/>
    <dbReference type="OrthoDB" id="2505422at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070692">
        <property type="term" value="C:CTDK-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032786">
        <property type="term" value="P:positive regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042326">
        <property type="entry name" value="Ctk3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28291">
        <property type="entry name" value="CTD KINASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28291:SF1">
        <property type="entry name" value="CTD KINASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022665547" evidence="2">
        <location>
            <begin position="23"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="214" mass="23332" checksum="B19B45756E561A70" modified="2019-11-13" version="1">MFSKASSFVLLLVAALFLGVHAIPATSKPALSPDQALSPDQSSSVTSETSSVEVTTFVQRWDSLSQSFTQTQSTFEQRVDVSVAYQSVQTLCQSYQSTVTEYQSCPSCSRGLAQSNFRTQFQQTITTMYTSFQRIIIVGQANYRAQWQTKFAQLFQRMSSFGRFCQTIALSLHLDLGALLKGLGINLNLFVNIGIDLGGLLGGPSGGLLSNLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAV4</accession>
    <name>A0A5B0QAV4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ino eighty subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAV4"/>
    <dbReference type="OrthoDB" id="5413003at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038014">
        <property type="entry name" value="Ies1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287:SF1">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="49231" checksum="2B9CD4807FCCF0C8" modified="2019-11-13" version="1">MLKSCQLPSEFGNEPESLPDIAKRSALGYRPPTTVVNLIFELFSVEPYVSMKYFPEGFVLHDLFSPTTIPTKLRAYAFLWLMHHFLEDPSALTDFQHESKVEAFQLARLEDIQPDSTLGDSPENIDTEEEIQWGKDMQTYRREFLKKWRGEEGIGFVDDLGTGFIAPLIEDSQLTKKQKIMVQNYAQSSAIEGDEGQTSQAQLAAKGSTPGSSIPPSALIGSAKFENRKTTTKEPEKKSKTVSKARMTHIERLQSALDAIVKYGDDSDLDGPDDMLTATQIAWRRYQTDVTEDFDVAYDSDDLIRQPNPTWKPKELKHRLLGFAFPVATANTIAPTEEEMKDKVIRYVGVKRRTPASNVPAGGTTGGSSKKGSSATPGVGGNEAGLAATGAPPNPASNPPKLKRKRKANQPPPDPASITGLPKPDPDQPAQLNTQGELPIHMLSRPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCG3</accession>
    <name>A0A5B0QCG3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCG3"/>
    <dbReference type="OrthoDB" id="10676095at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022748957" evidence="2">
        <location>
            <begin position="21"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="537" mass="61015" checksum="05B0D9E6620676F0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDN8</accession>
    <name>A0A5B0QDN8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_010176</name>
        <name evidence="6" type="ORF">PGT21_010642</name>
        <name evidence="7" type="ORF">PGTUg99_001371</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000293">
        <property type="protein sequence ID" value="KAA1111251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QDN8"/>
    <dbReference type="OMA" id="MHGKDVN"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:002045">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="31"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="247"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1073371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1076537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1111251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="351" mass="37046" checksum="C92CD1E0B1C064C2" modified="2019-11-13" version="1">MATDAATANGTDGVAAITDALEATTIQELGRKVFVGNLSFATKDEQLRDVFQTHGQISDVQIIYRGTRSLGYGFVTFATCEEAEKAVAATDKTEIDGRAINVEIAKPAPGTPGGAVPRAAARAAKASKARQTNGDAKTSDEDHDTDHPSPTGSKARRSRSGAGRGRSKRSRARRPAGESKPEANGDDHGAVTDASTHNGEDQPGKPRSTRTRTRRGKGAGAPGSVDGAKRERRPKKKGPPEGEPSQTLLFVANLPFDVTDEKLKEFFSSYQVASAHVVCRRYGSSVGKSKGFGFVEFVNEENQLKALEEIQGKELDGRALHVKIAVAEAKKEGEEATNGAPIPAPVNSAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJT6</accession>
    <name>A0A5B0QJT6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJT6"/>
    <dbReference type="OrthoDB" id="2498658at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022902726" description="Secreted protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="542" mass="63410" checksum="FDB403E1F6ADB51F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP52</accession>
    <name>A0A5B0QP52_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP52"/>
    <dbReference type="OMA" id="WANQSGH"/>
    <dbReference type="OrthoDB" id="2501187at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="454"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="500" mass="54230" checksum="65E4422F733AE84D" modified="2019-11-13" version="1">MAPSNSDSQTVKEEENAADEAQDSISLSIRRIEAIRELSGDITPLRPDGSNLQAWVNEIDEVLADVIDREKYLQSAPPVPFSKVQDKVARSLIYRTIPRELRYSLRDASSAHTAYESIARQFLRNTRTGHMSALVDLFNFRMEVTEAGQISLLYDQIYKGFHELVASGFTITEDAILGALFQIALGRSNLELYTAVSQYFDAKMSRGNTSVTSQEVAAVARMQLEHTPSITSASDEAGLFVPSGMTTPQGFEDANEEPREQPTARPNLATPAQHAAESNGNVNAPANFQHSVTPQQQQVQTNHSHPVTHPCMTQQPTGHLANDSSSAHVHQAAPLDPQFSNLGQPVDPNLNGNVYHNVWANQSGPQVPVHQQQANQPYSQQVPHPGAAECQANEAAQQLPPEPTATPAPAANQAIEHPDNTLHKSTPVNSSSHPLASSASGSGDRSTPHDPNLSKSSQPSHAASASSLKKHPIYGPNMELQSGKSANDTKKKSHHSKDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ47</accession>
    <name>A0A5B0QQ47_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ47"/>
    <dbReference type="OrthoDB" id="10270265at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022806640" evidence="1">
        <location>
            <begin position="22"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="124" mass="13714" checksum="C8CC28FE8E653200" modified="2019-11-13" version="1">MRLTAPMILTTFAILLEAVLSMDGVIPTETASTADEDLIWRALDVPTPPNPEEEPWHREDCKSHRVRWNAAHQFCYGCQTLIADPQSGYLACHTCRMIKPGSTRQINICTKASCLTSPLPTSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQJ5</accession>
    <name>A0A5B0QQJ5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQJ5"/>
    <dbReference type="OrthoDB" id="2505311at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="154"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="414" mass="47090" checksum="4637A9B855AC92ED" modified="2019-11-13" version="1">MTAGVLPPGNLQFSLNAHQKQMRNARLKERRKIKREKDSNENVTNAPPPSGIHIIRGRVQPIDLFPEITADLILRVNKYKGLVKAHEENPRKHAKPPKKQIFPRNPTNEENAAALKKVRDTFAQVNYGYTKIYDETTNQLVAMVHYLPLKTMDQQRLEDLNFLCLYLHRCKEFISRVASKNRTCGGVMWAIGWRKGYDGLEILGRYRCQKSIDKNPQGYEDLMSDSSRAGEILWDIFHGFGNVAVEKNKAHMDSYGIPSIADNNFPKNPNDKSPFGFASNLAFSSHGFYNHAHKDKGDLTELPLAFAMIVPTFKKTGKITFASDGYNVQNGQFIFRDIKVAINFHPDTACLIMFRAQEYVHCTLRPTESKCFTKLGMSLQVATRTSNACKNYLDGAYDDETDMYFGGVEDLLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSF7</accession>
    <name>A0A5B0QSF7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_003031</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2500603at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000009">
        <property type="entry name" value="Probable E3 ubiquitin-protein ligase HECTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000288">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010309">
        <property type="entry name" value="E3_Ub_ligase_DUF908"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010314">
        <property type="entry name" value="E3_Ub_ligase_DUF913"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025527">
        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06012">
        <property type="entry name" value="DUF908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06025">
        <property type="entry name" value="DUF913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14377">
        <property type="entry name" value="UBM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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                <person name="Silverstein K.A.T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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                <person name="Schwessinger B."/>
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    </evidence>
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        <source>
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        </source>
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    <sequence length="1284" mass="143416" checksum="B9A843CA793EC1FF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVJ3</accession>
    <name>A0A5B0QVJ3_PUCGR</name>
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        <recommendedName>
            <fullName evidence="7">KOW domain-containing protein</fullName>
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    </protein>
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    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVJ3"/>
    <dbReference type="OrthoDB" id="1688503at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1117318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14612" checksum="8DB7A418DD45800E" modified="2019-11-13" version="1">MAKFSKDVSSSTPKVAEGPFPSPFARKTDYHVSGLSKELRLSTTFDPCPSEKDDEVVVVRGAFKGAEGKVLIGIYRKKYVIHVDKVTRDKSKLVSTLPKVVISKLYLDKDRKAILGKEKIGEAQRRKCKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX38</accession>
    <name>A0A5B0QX38_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QX38"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023019789" evidence="1">
        <location>
            <begin position="18"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="96" mass="10899" checksum="B3A3285DF2D5341D" modified="2019-11-13" version="1">MQSLPIVCMGLFSLVAAQPPLPKYCWQCEVIATPKSRNKLSHLELDAGTAIKCWIQGCNEDITADRYYCSTCHHYAWYAATDCPNHKLLDPLYYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXY2</accession>
    <name>A0A5B0QXY2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXY2"/>
    <dbReference type="OMA" id="VEYAVGF"/>
    <dbReference type="OrthoDB" id="432536at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053275">
        <property type="entry name" value="Agnestin_monoxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38688:SF1">
        <property type="entry name" value="FAD_NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38688">
        <property type="entry name" value="PYR_REDOX_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="19"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="394" mass="43380" checksum="082D51FB8A4CB76A" modified="2019-11-13" version="1">MSTGSENPRAPKKEEQACYQFVVVGAGPAGLTAVANLIDMGVSRIAWIDLSFSGGRMGEKYREIPSNTKIQLFLDYVAASPTFSQLVQEATDPSNAYTALENLELDRGNQLSYAADLMIMLTRRLVSAHSDQVDTFQARAKSLDFVNRTDWMVEIEMMDSRCGHQAKTLKSRKVIFATGSEPVQPKEKAVPPIDLEVALSPSQLEAALEGIDLEKQTIAVVGSSHSAFLVLRNLISLSSQLRIVHLYRNPKLIFAEQKDGWILYDNTGLKGVVADWAKEEYPSLVADRRISRTQIEDSPGALSRHLESLASCSGVIYAIGYRPAPTPTVTLDGLEQSLKFDHSTGRFDQLNGLFGCGIAFPQRTVDPLGNVEHAVGIFKFMKFLKAVVPLWIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYT4</accession>
    <name>A0A5B0QYT4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004461</name>
        <name evidence="2" type="ORF">PGTUg99_008332</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000250">
        <property type="protein sequence ID" value="KAA1118458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYT4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="84" mass="9699" checksum="0F6E7CB687632D4F" modified="2019-11-13" version="1">MRQECRSLHKYVLSVNGGNVPNDAYLCPKDFDKSWTHLGRNPHHRRGARLPTYGDLEKSVLADSEYLTHEEGYITGKHQMWTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0U4</accession>
    <name>A0A5B0R0U4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0U4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="201" mass="22363" checksum="3385AE1379FEE049" modified="2019-11-13" version="1">MQTRIDNAHIHRSRVLPKAQSKRLGSKSPSRSASFRPSIQGYYTSIYMQNHTSPSTANQQRERLGPDLNQRTVSPSQTARAADEWDLTISDPLCSPLSFKPSTLELKIFICLHTSPDWPSLSPNEFDCTPFHRVLSSLLLPIQPLLESLSPIVVGTKPKIKAHYYLSLLGSHHIAIKLLLDDESDQDLHPTTTPDGSDLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1B4</accession>
    <name>A0A5B0R1B4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1B4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="515" mass="57225" checksum="C30676F46BAEAFF0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1G0</accession>
    <name>A0A5B0R1G0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AMP deaminase</fullName>
            <ecNumber evidence="4">3.5.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myoadenylate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">AMD1_1</name>
        <name evidence="13" type="ORF">PGT21_004683</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.6" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1G0"/>
    <dbReference type="OrthoDB" id="1723809at2759"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00663"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01319">
        <property type="entry name" value="AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.20:FF:000001">
        <property type="entry name" value="AMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000035">
        <property type="entry name" value="Probable amp deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01429">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001251">
        <property type="entry name" value="AMP_deaminase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="819"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="820"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1118754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="925" mass="107100" checksum="3C9E65C927A5E956" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1V6</accession>
    <name>A0A5B0R1V6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1V6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="231" mass="26597" checksum="EBAAF79B6D6B7BC1" modified="2019-11-13" version="1">MDFPVVVTESTAYGNDIGRKFISEPGVRIRDCESSAPQTKEEKIKDYIATVHQLRKQEGIFLEMANHIQSQRNSIGGDFVAMTEDLLKIRKEYDHRIIDYKDLAKTDSLENIKQLEDLHSLILDEMIYRIYISNDIKSNKITLQMHNLYTKVLNKNIKTLDLDGNSDSEIFKGEDLPQDLVQTMKNKLLEFQIINLNKMLHKIAPLNTHVQSESCDADKITNQIALMIQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R5R7</accession>
    <name>A0A5B0R5R7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">VMA3_2</name>
        <name evidence="14" type="ORF">PGT21_037305</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Vacuole membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R5R7"/>
    <dbReference type="OrthoDB" id="1744869at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000001">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="68"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="143"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1120244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="235" mass="24385" checksum="EE9F50E84E0ADF5A" modified="2019-11-13" version="1">MGRRSQNIRKTPARPGPIPGQSQANPTAIDHLNPSNHQSSLFLFLQSNAALRVLQKTHTIRTVTFEHGCTAAIVFTCIGASYGTSKSGVGISAMGVLRPDLMMKCIIPVVMAGIIAIYGLVVSVLISGSLQSPMPLYQGFVQLGAGLSVGLAGLSAGFAIGIVGDAGVRGTAQQPRLFVGMVLILIFAEVLGLYGLIVALILNTRGSQHPPCRFGSPGNGSNVVAVNAIILGEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RCL3</accession>
    <name>A0A5B0RCL3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023540</name>
        <name evidence="3" type="ORF">PGTUg99_022410</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000208">
        <property type="protein sequence ID" value="KAA1123506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RCL3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366622" evidence="1">
        <location>
            <begin position="33"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="123" mass="13967" checksum="8331BFD80535ED9C" modified="2019-11-13" version="1">MWSYTLTARVTLINALIMYSLVKAPLWGEVKARPCSYCFVPNIRAPTPRVENTNTLLPIGRDDKPCWNPNPPCGESIPVILFYCNACYTTAYAPLRMCSKAHSLHEVYVNNSRREIIEATLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RQP1</accession>
    <name>A0A5B0RQP1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020526</name>
        <name evidence="4" type="ORF">PGTUg99_004031</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000155">
        <property type="protein sequence ID" value="KAA1127662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RQP1"/>
    <dbReference type="OrthoDB" id="2510530at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1127662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="355" mass="41247" checksum="7A335E7EDF26AFD1" modified="2019-11-13" version="1">MDSFSSRLASGYRMNLHFQRILVLGLINLTSILNMALGIPMKGNASMKTFDVLRNHPSRTGERPPILPARFCNNVLRTERDHRESAAAFATLCSRVENPAIVRSSPSRITRVEKEHQQSSEAIKQLWLREYPRIKMVQDFYDVEQSYREARARKPNQKQQKILNLLEEQLRRIQSLLLEQKYHIHGYQFPKGLLVKLFRNPSGENYGKDILSALKDILLASTHGDKNDSLRVMNLCRKSAFLAINLVMEYAIASYDDLRLIFKDDKLAYATLAYRFLFFDPQSTASQLAWKNAQIVLLNDRKILLKARIRGRKLQAAVKKMKQLREIREKQKMIEEERREKRLTNGVQRMLSNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RU06</accession>
    <name>A0A5B0RU06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023876</name>
        <name evidence="3" type="ORF">PGTUg99_027479</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RU06"/>
    <dbReference type="OrthoDB" id="10012223at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF2">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="312" mass="35947" checksum="B952B88E1119648A" modified="2019-11-13" version="1">MPQELLRFPTSYLTVGIYRLGTDPKINRAIVQEFSTIVKRLVILSIVYCALSYPVTKVWVKYFLLSKRAKPADQDQGGNIAETVGVIGGMFSLLNFTTLSLVLSQLSSIIEFFLLRKLKKTRAKVYQFTIESRAKSEQFWIPYVEEWDHPPLEKAQRRISKWKWYKTITGPIFRLLVLKVILLPLNFVPFLNTIVGSFLVSLTLSERLLEPYFKTKKMSELEQALFMVERENSLRWLGFFAAVLERIPILGILFSFSNRIAFAMFAHDLEKHQHAVKQGLIPKQPDPYRSKTAAIELDLPSDAIGNFPKKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S5Q9</accession>
    <name>A0A5B0S5Q9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022566</name>
        <name evidence="3" type="ORF">PGTUg99_017745</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000076">
        <property type="protein sequence ID" value="KAA1132809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S5Q9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="104" mass="11729" checksum="66D4F7023EB3D6F6" modified="2019-11-13" version="1">MWCNANQISAVHYHKHLWFGTDSKQLQKSVSDPFQDSARGPFPKISDRVKIPQYPPAFRQTGSDSRDTFNEGSAEDRATSGSFCMYSCAVGSQPFTIEFPENFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SB65</accession>
    <name>A0A5B0SB65_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-deoxy-7-phosphoheptulonate synthase</fullName>
            <ecNumber evidence="4">2.5.1.54</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3-deoxy-D-arabino-heptulosonate 7-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">DAHP synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phospho-2-keto-3-deoxyheptonate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_027443</name>
        <name evidence="15" type="ORF">PGTUg99_012676</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SB65"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF12">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, TYROSINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="13">
        <location>
            <begin position="117"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="24"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1072079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1135198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="443" mass="48349" checksum="5B16FC790778C84C" modified="2019-11-13" version="1">MTNTQAVPSLALSASMRLSPLIEEETKADRKYPIPSSRQTDQSELRTTDSEDEEDDEADRDTDEASNWSTSTSLSIGEGKVNGLVVESYDDLVSPVEVKAEIKISKCAEKLILHSRQAISEVISGKDDRLIVVVGPCSIHNVEAALHYARELKAVESQFPSLILVMRTYFEKPRTTVGWKGLINDPDLDGSFQIERGIRTARKLMAEITHMGLPVATELLDTISPQYLSDLISWGAIGARTTESQLHRELTSATGHPVGFKNGTDGGVKVAMDAMQSARASHAFMGVGPHGRASIVRSRGNNNVHVILRGGSKGTNFDAVSVGETRSSLMKMQPTFHPSLMIDCSHGNSSKDYRNQPKVIKSICEQLSNGSDEGAISGVMIESNIHEGRQDIPKEGPIGLKYGVSITDACVSFEETVKMLQSLEEAVLRRREILGSRRSSLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SDU2</accession>
    <name>A0A5B0SDU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017095</name>
        <name evidence="3" type="ORF">PGTUg99_032356</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SDU2"/>
    <dbReference type="OrthoDB" id="727118at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1136128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="143" mass="15357" checksum="CE6F109F8C0F5761" modified="2019-11-13" version="1">MMGAGMAMMMGDAGIGFGTTAIGIGRDPKARAKSRDYLKQCLQEINYHTSNPILSKTQAVQHSGPNHPNQANTNHFQAASYLHQPGNNQQDNLSPNPIHQPLNHSGAPQDQSQQSALYQALTIDNQHPPNVSSFAQHSGAPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LHL0</accession>
    <name>A0A5B0LHL0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein phosphatase PP2A regulatory subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PAB1_1</name>
        <name evidence="2" type="ORF">PGT21_000584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000206">
        <property type="protein sequence ID" value="KAA1063849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LHL0"/>
    <dbReference type="OrthoDB" id="2502349at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046820">
        <property type="term" value="F:4-amino-4-deoxychorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008153">
        <property type="term" value="P:4-aminobenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF18">
        <property type="entry name" value="AMINODEOXYCHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1063849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="156" mass="16639" checksum="B58F01743A82389B" modified="2019-11-13" version="1">MITDLIRHDLLGFCVPGSVRVARLFGLESVATVHSLVSTVQGSLRPHLNPVDALAAAFPPGSMTGAPKLSSIEILDRLELHHPRGPYSGILGFIAIDGRSDMSVVIRTAIIRNNHITVGAGGAITSLSKKENEWNEVRLKVDAVQRSLKLSPSSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LI24</accession>
    <name>A0A5B0LI24_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LI24"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="215" mass="23923" checksum="079ECA1076F517FA" modified="2019-11-13" version="1">MTFNTEWIPGATFSGSAFSDFVNTTPAANPLPSNDVDAADLNDSDSIAPDDESNLANDTESDNPNDEIAVLTNPNPVIFVANDSPPAYQLAALPLGSPENPIEIEDGDVANEPRNDEETYERLNSPRFDPVEIVPFQTTVVDEEDVLRARERINRWFNNHIRHNSQRVLRGRSRRARTAAGPVEPYWTVNELMDAVLEELDDVLNNASRVELIHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJ47</accession>
    <name>A0A5B0LJ47_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJ47"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="56" mass="6500" checksum="ADF9422AF4487318" modified="2019-11-13" version="1">MSSSDEDPFRLSWGKRNQARPDPARPTARIDLKPKLFQLEVWRSPAAWDEEELTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNV7</accession>
    <name>A0A5B0LNV7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNV7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022666585" evidence="2">
        <location>
            <begin position="23"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="353" mass="39978" checksum="47014C1F624405D0" modified="2019-11-13" version="1">MLSLLNPSTLLCWSSAVASAAGKEKQQTLPDRCRARINASRVKRHQPPSPLCGISPLQQVAPAPNQATTSSLDQSTVTPPLRNSRRRERLPFIDDEAKKSALDDWTAKLRSFPTANPGGSRHPRTYTLIGRGNLIPSPQRGTLSRPAAASQGKFTRRERTPLAPTQAGRPQQEGQRDRDYPARVMAIRERMPGSRMSTSSQAKRPRQEEPSDRIDPYARVRAIRERMPGSRMSTSSQAKRPRQEEPTDRIDPYARVRAIRERMRGSRMTTSSQARRPRPEKPSDPIDDRIDPYARVRAIRERMRGSRMTTSSQARRPRPEKPSDPIDDRIDPYARVRAIRDGMRGSRKSTKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRT0</accession>
    <name>A0A5B0LRT0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neutral amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">AVT3_1</name>
        <name evidence="9" type="ORF">PGT21_026163</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRT0"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF666">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="532"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="571"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="609"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="642"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="684"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="755"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="788"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="428"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="360"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="377"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1066244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="833" mass="91294" checksum="43947926D417B10B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSN9</accession>
    <name>A0A5B0LSN9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="5 12">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_034008</name>
        <name evidence="16" type="ORF">PGTUg99_012948</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="16 18">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the NadC/ModD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="5 12"/>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSN9"/>
    <dbReference type="OrthoDB" id="10067394at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000090">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase [carboxylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.20:FF:000003">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase [carboxylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="14">
        <location>
            <begin position="41"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="13">
        <location>
            <begin position="117"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1066584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1081814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="306" mass="33494" checksum="3D126A593FC18D86" modified="2019-11-13" version="1">MNTSALGDPAHLLPPSWKSVVTEWLREDTPSFDYGGFVVGESQQEAQLLGKSQGILAGVPFFDEIFHQLECRVEWKFKEGDLIPGGRTVVAIVTGKARCLLIGERVGLNLLARCSGIATQSHQVLKLARDHSYKGLIAGTRKTTPGFRLVEKYAMLVGGIDPHRYDLSSMVMLKDNHIWSKGSITAAIQAARSVCGFSLRIDVEVRDEKEAKEAIEAGADVIMLDNFTPENVKTSARSLKQSFQGTDRRFLIEVSGGLTEQNISSHLCHDVDIYSTSSVHQGTRTIDFSLKIVPTVEGFTGAISSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LT74</accession>
    <name>A0A5B0LT74_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_013155</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LT74"/>
    <dbReference type="OrthoDB" id="2514764at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022727804" description="hAT-like transposase RNase-H fold domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="68"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1067661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="43459" checksum="727305BB47652C37" modified="2019-11-13" version="1">MHARCFCHKLALIVNAALAALLLKTLPPGKSKQSVLGYFPVLGKVAEEDEANEDQAVVVTKTSLPVNVQNDPASVSDSGTDSDYGNADESLSEYEGSVDSDDETEPTKTKSTLKTSASTKYKNSTKLLELTQKLKVAPLIAGYGSRWNIRYQSYQKAINAREVIDHILKEDQEQNQAGLFADVLFSPRDWKEIDNLNAQLEVFVKLTAEMEGDSATGCHVLPKYLELRESLKEKLELSSDTDSLYPMLHAMLQRVDKYLKEAMQCHTLVLATLMHPCFRMHLFELVFGANSPEVTTILELLKDEFQRTKAKQQELAKNKNPIDPDVFIVDKPLDLPPTGSSLMNSLASRMAVQPTAEANEVESYLQADLSFNEGDIERKTTPLKWWQVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LTV5</accession>
    <name>A0A5B0LTV5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13 14">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="3 13 14">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MVD1_2</name>
        <name evidence="17" type="ORF">PGT21_008258</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:23732"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 14">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="3 13 14"/>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LTV5"/>
    <dbReference type="OrthoDB" id="10253702at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000018">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000005">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 13 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 13 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8 14" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="16">
        <location>
            <begin position="9"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Mvd1 C-terminal" evidence="15">
        <location>
            <begin position="184"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR015950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1067526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="405" mass="44693" checksum="5E1C0D35E63C5489" modified="2019-11-13" version="1">MVKEITCSAPVNIAVIKYWGKRDKKLILPTNSSLSVTLDQHDLRSTTTARLLDPSHKNGEVGEEEDQLWLNGARQPIEKDSRLSNCLKELRKLKAQFELQDPKTEASLPESRRPLLIASENNFPTAAGLASSASGFAALVYTISKLYELPIEMTELSKIARQGSGSACRSIFGAERSDWPDLEALICVVSDRKKGTSSTSGMDGSVQTSELLQHRIEKVVPERMKRMKSAIKQKDFDSFAALTMADSNQFHAVCLDTQPPIFYLNDVSRSIIAVIEELNRASKAEGDGCLAAYTFDAGPNAVIYAPKRNMRKLLNLILHYFPLPDSDPFTDPKAYFDLSKETPGQLALPIHFNHNVSPVWKQGSISRLIHTQVGDGPQVLNNQLGHGLLTVDGLVKSLKKPTNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXM2</accession>
    <name>A0A5B0LXM2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024767</name>
        <name evidence="1" type="ORF">PGTUg99_022839</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXM2"/>
    <dbReference type="OrthoDB" id="10606196at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="130" mass="14579" checksum="53705AA50E2862DD" modified="2019-11-13" version="1">MQRFLACRCKKPSPSCNPAHTPKTYEQDHQKAFRCPRVNYEPLDERIAVCGGVSKTDPNKYEMTEANTVVDDKRAHNCIGAPLQEIRCCRKSAFKFPQNPKHGFKTITVDLSATKDDLNCVAGRQEGAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0R0</accession>
    <name>A0A5B0M0R0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_021722</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0R0"/>
    <dbReference type="OrthoDB" id="165382at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF65">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1069344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="169" mass="17940" checksum="5E1294DED8949AF6" modified="2019-11-13" version="1">MMPLNSDPSTSTLIGVGISVAGNLMISVALNVQKLAHQRLEPSEPRPAKKPSEATPLMIDEPLQELHAPPTDLESPRSGSPASSSSSRAQSYQPNDLAYLSSPLWWLGFVIMSTGELGNFVSYGFAPASVVAPLGTVALVGNCVAAPVLLGERFKKRDWLGIGLVIMAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2C2</accession>
    <name>A0A5B0M2C2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2C2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="181" mass="21244" checksum="A85F502073D43974" modified="2019-11-13" version="1">MRTTRFTVRTADRSQILRRQVHAARIAETLARIRRQEQLDRQREQLARPPAFQNVLDEPDTNFEDQPDEGEHNQEDETEENCVWVDLLEDQPDEIDIAIAADRERHRQQAREFNWAVLLHHLHATYMKLKAITKDWTGSNSYNSFTSCSATCSREFHRTVNLVDIQGEPKQLTPIHSPIHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M691</accession>
    <name>A0A5B0M691_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_003801</name>
        <name evidence="8" type="ORF">PGTUg99_003935</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COA8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000015">
        <property type="protein sequence ID" value="KAA1136915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M691"/>
    <dbReference type="OrthoDB" id="6246201at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018796">
        <property type="entry name" value="COA8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31107">
        <property type="entry name" value="APOPTOGENIC PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31107:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10231">
        <property type="entry name" value="COA8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1071334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1136915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="230" mass="26900" checksum="F1C7AEA07E87C08E" modified="2019-11-13" version="1">MLTRTVQPEWWFISKRVVRLYSTGPSTDSGGRRGRLTRRETLVDIGRGATEDQVGPPCARSNLRPIRYSARFSSPTTSSPPTSLVESPHRHPYSLEEFRAEPVELSGRLAELRARLELEDLQWSMFRNRVDKLNQAFWETQSTRFEALEQKQRDAALAKTVNHPPESVQERVNTRLDQFYASWLIDQKKLFMLYNWRWWGLQAALLKGGWLAQVRDLKWKLACWRDTILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7M4</accession>
    <name>A0A5B0M7M4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Anthranilate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021401</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7M4"/>
    <dbReference type="OrthoDB" id="1865897at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
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            <end position="194"/>
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            <end position="514"/>
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            <dbReference type="Google" id="ProtNLM"/>
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</entry>
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                <person name="Sperschneider J."/>
                <person name="Matny O."/>
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                <person name="Schwessinger B."/>
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                <person name="Figueroa M."/>
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            <strain evidence="2">21-0</strain>
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    </reference>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <end position="586"/>
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    </evidence>
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            <dbReference type="EMBL" id="KAA1072618.1"/>
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    </evidence>
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    </evidence>
    <sequence length="1088" mass="116281" checksum="19B302EB7D0E8436" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAJ5</accession>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">21-0</strain>
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        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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            <end position="902"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
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        <source>
            <dbReference type="PROSITE" id="PS50217"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1073259.1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Visser B."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <dbReference type="AlphaFoldDB" id="A0A5B0MC73"/>
    <dbReference type="OrthoDB" id="10456545at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="57" mass="6762" checksum="D10614CBE4FC2856" modified="2019-11-13" version="1">MSERHSRTSDSSRFDSPNTVTKNLLVMLHPLAKEFEERFMNSVPVKYRESYCVLLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCV8</accession>
    <name>A0A5B0MCV8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016143</name>
        <name evidence="4" type="ORF">PGTUg99_013155</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000271">
        <property type="protein sequence ID" value="KAA1115880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCV8"/>
    <dbReference type="OrthoDB" id="2495291at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061672">
        <property type="term" value="C:glutathione hydrolase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01908">
        <property type="entry name" value="YafJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000060">
        <property type="entry name" value="Related to DUG3-probable glutamine amidotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052373">
        <property type="entry name" value="Gamma-glu_amide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1115880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="333" mass="37928" checksum="19104F7640854033" modified="2019-11-13" version="1">MCRLLIFKGEPIGLSHLITKPSHSIINQAFDCRLRLDARPVNADGFGVGWYDTELEDSVPCVFQAITPAWSNRNLHRLAEKIRSPLVFAHVRASTTGALSEENCHPWIYGSLLWMHNGNISGFTKIKRHLQNELSEEFFHFPQGSTDSEWAFALFLNELSKIADPKATSFPYVTLKEVMLLTIEKIKCWWVQAGVQEPCRMNFAVTDGRSVVATKYVTSSTEDAASLFFSTGTSFEPSKQVNIPPPTYLDDDHQPRPAEQYRMQKYDKREKIVLIASEPLTFEKTDWIEIPCQTLVVVTPKMNVLQFPIIDEFVDNVYKTGISLTSKSFYHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDK2</accession>
    <name>A0A5B0MDK2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDK2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="54" mass="5875" checksum="AF4823D1DC31E0C7" modified="2019-11-13" version="1">MTGQTDINGDVDPHQGTVKTGLEWGCFLPLMNGQTVIDGVFDPTKDHTHVLHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEC1</accession>
    <name>A0A5B0MEC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chromatin modification-protein VID21, variant 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">EAF1_1</name>
        <name evidence="5" type="ORF">PGT21_026886</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEC1"/>
    <dbReference type="OrthoDB" id="5364245at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459:SF1">
        <property type="entry name" value="E1A-BINDING PROTEIN P400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459">
        <property type="entry name" value="E1A-BINDING PROTEIN P400-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSA" evidence="4">
        <location>
            <begin position="370"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="127"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="651"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="681"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="757"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="985"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1017"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1119"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="651"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="762"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="786"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1042"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1062"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1146"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1165"/>
            <end position="1181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1075002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1187" mass="129660" checksum="2375F2ECA9661BBF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MF57</accession>
    <name>A0A5B0MF57_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MF57"/>
    <dbReference type="OrthoDB" id="10341853at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="293" mass="33412" checksum="A1274174878B5754" modified="2019-11-13" version="1">MGGLYGTWRFSTSIELLSREEQLQIAIARIHQEEDDTLAAQILVNKALREYKEYKREQKLRMSTSSITASANPNTSAGVPDNHSAVDPIGNPDDTSVDELDELLNYPTLPDRSQLRQDQGAPLPLEDRVRQLSMLQIKKKTAETTDYQITNDSQNKTRDEAMDLDDPSTSLEDTPKRPNSPDIVTLSKKERIRLLIKEHIAIWKKFKLKKPSGATPELRTILHQAQDSQKALQKLITKEELEGYVKGWNPWDAKRELFPPPAVGRYATLRFWPKKRSVAALFFYPKTVVLAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFP4</accession>
    <name>A0A5B0MFP4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFP4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="99" mass="10170" checksum="8E9A343671523701" modified="2019-11-13" version="1">MTGWLALDRMEKTLTHLVSAAANGKAQSDEHPPNQDSVRSLLAKARPGAGLSLGERGPAGRPGQAASGSTAALRGPVASSSFEQAVDNDSCGRRSQELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFT9</accession>
    <name>A0A5B0MFT9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFT9"/>
    <dbReference type="OrthoDB" id="2496590at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="407" mass="46932" checksum="CE6B5D12411ADA1A" modified="2019-11-13" version="1">MFTKSPQEIEQELQAGRDLIASLQIKSEPPASPLQPSKVWKQSAATRLAFIKAFKEAIPLIENGPEGQAWQELLLRLGGQAHDYRVERSLVPRLRQHLVEISDVFVPARLQEVLSSKNPTSQVGLMKLVATEINQLKSNVTSPCSNQTSKISTYALDPYYFPDLPWEVDQIRDGIIEAFKEYIKTLKAKEPPSGDMRKPSLRELLESVDRMHGDFEDPRRVLKAKWKYVTREIEIMHMTVGNCLKPRAACKNTNPNGRDDEPMVHPWPSLEEMNKADIQTFQAYIPLLKICRLLLVKLCKTPASDQLLIYKMSLADLKEFYNKTEGLEEDLRKFTTMILERLTSRAILQLDAFLVWFNYIIKTVETHLATLDSPADRAHLKKSREWCLMWKIQYQIAIRNFAPKRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGA7</accession>
    <name>A0A5B0MGA7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">U3 small nucleolar RNA-associated protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_031202</name>
        <name evidence="8" type="ORF">PGT21_031943</name>
        <name evidence="10" type="ORF">PGTUg99_001090</name>
        <name evidence="9" type="ORF">PGTUg99_002307</name>
    </gene>
    <organism evidence="7 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000399">
        <property type="protein sequence ID" value="KAA1090268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000111">
        <property type="protein sequence ID" value="KAA1130193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGA7"/>
    <dbReference type="OrthoDB" id="2502854at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1075208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1081241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1090268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1130193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="39983" checksum="73B9A831DFECDAFB" modified="2019-11-13" version="1">MARFKLDVQKKQHRERSQPLQRKRLGLLEKHSDYVQRAKDYNSKKDRLQKLRLKASLKNQDEFYFKMINSKTVKGVHYNFNKANTPLDNQLVKLLKTQDFNYIKTCRAIEENKITKLKERLGSMIIRVQSEESGSSSDLKEQAIQLINQSITATHAKNTANSDQGGSELDHDDHALVKPGKKTIFVDSVEEVKNFSITSLGKKTKKDKTQSASKSSSKKTKGKFTEEFSDAEDQLTPDPEKHMRKLMTEFQTRLTRLRVLQTAEREMELKKSLMGKGSKFLKFRSSTQKPKQDGLVNGRDLSWYDKLDRNQIIEDDLERERLEKLNSGIKTGAQVWKWKQERKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHI0</accession>
    <name>A0A5B0MHI0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_006111</name>
        <name evidence="3" type="ORF">PGT21_006710</name>
        <name evidence="5" type="ORF">PGTUg99_014164</name>
        <name evidence="6" type="ORF">PGTUg99_017925</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000075">
        <property type="protein sequence ID" value="KAA1132857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHI0"/>
    <dbReference type="OMA" id="HGMPYIP"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1076405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1132857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1135554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="205" mass="21690" checksum="1F083725E0B4674E" modified="2019-11-13" version="1">MPAKVALLTYSMYGHVDILADSIEEGLKASGVAVSRFQFPETLAPEVLEKMHAPPKKNIPVLTDPTVLKEFDGFLFGFPTRYGRTPAQLSTFFDMTGGLWQTAGLYGKFAGIFTSVASQHGGHETTAFTTIPFFAHHGIIFVPLGYTNPNLTDNSEIMGGSAWGASTVAGGDGSRLPTAKELNIAVGQGKSFGGVVNQYVAGAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIF9</accession>
    <name>A0A5B0MIF9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA wybutosine-synthesizing protein 3</fullName>
            <ecNumber evidence="2">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_006013</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TYW3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIF9"/>
    <dbReference type="OrthoDB" id="48625at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1960.10:FF:000003">
        <property type="entry name" value="tRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="12">
        <location>
            <begin position="23"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1076402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="307" mass="33646" checksum="68F215ECAA5D2161" modified="2019-11-13" version="1">MDEKTVSEYKNFEVEREQIALNLAELKDKSTKGTIDEPIRPFLDLINAQPDWISTSTCSGRLVSYLPGASAEAGSSDPSDHSQRVRQAVNGKGGGTWLFVSHSTLETNQLANPIQTLFGDSYRIKSSPNPDSWTCDDSSSLVVHFVYQPPVFHLLARSVTVAVPILTTVIGAGFRNSGITVGHRGRVILAIRASTGGLDVPIAIQKCSEKSTLQLLLPVDYLSKLLLQGNQLMHQNQAKLERLIEALSNSFNAPSLKSINQWESAEDRRQRMRLEGLAAREASLVSSQSNPTQTSANDEFPELSLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMN8</accession>
    <name>A0A5B0MMN8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMN8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="8429" checksum="262DF1E22DD69456" modified="2019-11-13" version="1">MMIDSSNSHTLSKISGTTRGTRRSSYVLARAGPVSTSYPPINHLSIIHPAIGLSIGDLPDYLLDDRILELMSKPPPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQD8</accession>
    <name>A0A5B0MQD8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQD8"/>
    <dbReference type="OrthoDB" id="2504409at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="231" mass="26094" checksum="6A16AFA3A9F4FB9F" modified="2019-11-13" version="1">MNLSQISDSEDLTDKPTIGRPSRPSSRQRKRLTAMPLIDLSQPTDADGRPIQFEGGRAKLVSQPNKPKKRMMAAPDLEQRHREAMRRLQAEATEHLIYAEWEAAHQRSNPHFDPPKLNRMPSRRAPLQKLSDFARKSVSNLSVRTTMVESPSRTVASRSNTIDKSRSIAPKNPECSRKSSTRFTQMIKKFGSLKRSPSSPKMFSQNSEASMSRKSLLTSFRFSASTSAFHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQV0</accession>
    <name>A0A5B0MQV0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQV0"/>
    <dbReference type="OrthoDB" id="10313259at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1078424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="235" mass="25492" checksum="BEC41BD0AA179FEE" modified="2019-11-13" version="1">MCACVRNCACKEPHVLETWILFAEGPGPIGSLNDNSLEAPYQSSLIVKVQLSAALLSILAFQSTAKLGLMFRWLILCLFVAGKCHAVEYGSPKLGSDAPQELSASLGSYHSHSSQQVDPHELHIIIDDPEETHDGKPHDAHETPSAIAESQSRPPESTTVTTLDPGPPEVKRFSRQATRQNSGRNGGGERAVAESSECTECLKSYLLSECGLKIISVSVAVGLMLTFIILHEHWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MS75</accession>
    <name>A0A5B0MS75_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025130</name>
        <name evidence="2" type="ORF">PGTUg99_020535</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000339">
        <property type="protein sequence ID" value="KAA1100729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MS75"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="89" mass="9599" checksum="6F486774A7DB61A9" modified="2019-11-13" version="1">MGPQATSMKESIEHFVAGMATPVAPFSHDGALSARWPAMGLAEERFPALLIVSHQGHCLTTTTAIGNGTFTYCLNLAALEHRTEEAYEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTX4</accession>
    <name>A0A5B0MTX4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTX4"/>
    <dbReference type="OrthoDB" id="10261753at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.540">
        <property type="entry name" value="TAP42-like family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038511">
        <property type="entry name" value="TAP42/TAP46-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04177">
        <property type="entry name" value="TAP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="190" mass="21237" checksum="5F4E3B931ED0233E" modified="2019-11-13" version="1">MENTFVSLERDCRGQRSRLYSSGRSKEDDDSLAERVNGSDDDDDDENESESRSTHLTRLKFLYLKAHQELGSIEAELEILGQAAQMSDLPSKKVQREEDDLSWRVERISTSADGPLVSPSGKVLRPVTILPSHSFATVASTNRIKLRAEVFRPDWTNNASWVTSCPAAAPSKLPKRLPPNALKSTLKRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MV57</accession>
    <name>A0A5B0MV57_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023813</name>
        <name evidence="3" type="ORF">PGTUg99_003219</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000013">
        <property type="protein sequence ID" value="KAA1137011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MV57"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="620" mass="69400" checksum="0BD8991F43FA7CBC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWG1</accession>
    <name>A0A5B0MWG1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin carboxyl-terminal hydrolase 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">USP7_5</name>
        <name evidence="4" type="synonym">USP7_40</name>
        <name evidence="3" type="ORF">PGT21_009298</name>
        <name evidence="4" type="ORF">PGTUg99_025031</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWG1"/>
    <dbReference type="OrthoDB" id="2496844at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="186"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1109271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="431" mass="49019" checksum="57B2DBEC02A5CD93" modified="2019-11-13" version="1">MLQDSIIRRTGGQFGAQENKKPTLNYEASKSLLPSNFPVERGQFGASMQDPAWEEEKHLSDEENDTMSDEETEIDENDPYVLDLPQDSCATLSERARKTLKQTLFLSKSYFTSSKPFLLRMSQKKKNIPFSIELQTFASSKTVIVTAGGCAKKVLFLVQFHPHDSSDPTFNHLSELIEDLYIMSNHRSNLKNKNKISGRMSGIGFRGGYEKGKSAGSYAVRKDLKPSQLELETNLQNKLPRHNQFIADRFRHFSEEAVAKNKQAMKEFGIPSWSDEAWESYKNDPTPLCSNVIVTSNDFSNKPHCDKDKNIFTYGIFSYIDRSTGTPILPPSSTRGHGIRFPDYDCNINFGTTPGIIEVLWKSNDICHHTIGPPEELKSTKKSTHFGCSFQISHRLVARALKLKCLPEEEKKKRTMTREERSKKKVSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MX88</accession>
    <name>A0A5B0MX88_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MX88"/>
    <dbReference type="OrthoDB" id="96345at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10846" checksum="C2B6011B62C9D5B6" modified="2019-11-13" version="1">MSSSPPPPRVHRREAGRDHRWDRDAINGGPSSLSILLNWLTTPGNYSRWNTPGNRDREPIGMEILELMENEGINHRHVWGTTDVSFLLFTIVVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXK2</accession>
    <name>A0A5B0MXK2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mitochondrial GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MTG1_2</name>
        <name evidence="5" type="ORF">PGT21_028656</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXK2"/>
    <dbReference type="OrthoDB" id="269151at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01856">
        <property type="entry name" value="YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:005958">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782:SF4">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="4">
        <location>
            <begin position="25"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="421"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="452"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1081063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="462" mass="51113" checksum="D00B271BFC792474" modified="2019-11-13" version="1">MTIQRSFTFPSVLPTWHLGHMVKAIRDMKQRMNDVDVVIETRDARLPLTSINPVFEEILKGPSVKNRAPSASSSSPIRLIVYNKKDLADPRLEKPLKDAFKKHGQTVFFTNARIDEDVRQILRLSKELACPRILSRYGKAAPSSETISTNLVQSQSRKKSSLSSLDPNRGIRIMFCGMPNVGKSSLLNALRRVGCQKGKTASEAPMPGHTRSIGGMVRVSEGSKQTAGWPVYAFDTPGIMVPYLGQGEEAGDRAFKMAITAGIKETLFDPYDLAAYLFHLLLLRHSSPFDAISRISEVLKLPPTFSSSIKDHLDASQPLPTEQLLSGIADRIKAVEVGGVPNLEAAAQWMLKCFRDGRFGGWTLDDVGQESIGAKVCNSSETSIIRLIESNSQTNQPIDDRALTENSTHIEGCISSYFASDRTSPKSKTAVKMEARRAKKDVQRAQQELKPIKHRPPQSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZX4</accession>
    <name>A0A5B0MZX4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZX4"/>
    <dbReference type="OrthoDB" id="10402992at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="14974" checksum="22ABA1F941A63E1B" modified="2019-11-13" version="1">MAPASNPPPSAKSTPEPSKRTKSSGVNQSVIASSFFGPADGNGKRACLIDNCGNTLVSNKSTKAIFSNFNTNIINWIIEDQLPSAKVKCPAFIKMIHGLDPGITIFSEHTVSGQIKALYADNLKVWLESITWKKNGLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1E3</accession>
    <name>A0A5B0N1E3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023916</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1E3"/>
    <dbReference type="OrthoDB" id="271553at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="26"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="349" mass="39454" checksum="DDA6432C86AA4692" modified="2019-11-13" version="1">MFSLVESITSRFNLTNHTNADRPLNTQVLYSSLSSTTKKLANQLSTSLQSSSQHLHSTLESLDHLDLDQLIIQTPVDSTYQLAIFLLPSYNVESPVDSVLAQFRDALFDFRLSKNSLAGLSFAVIGIGHSDYRDEFCKQAIELDEILEGLGARRLLPLQKIDVAVDPERDLDHYYHSIIQKTFHPALKSFSELPTIKSQEIKKYSAENEEEEEEEEEEEQDEVDNSDDEFVDYSNSKPLSKAEKARQKSKRLLNKKINQGEKKTNGTQDIEDLGVGVPQIENLTSNSKDDAENQMIKKDMISPMTRKSLTKQGYAIVGSHSAVKTCRWTKASLRGRGFCYKHAFYGMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1T3</accession>
    <name>A0A5B0N1T3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014452</name>
        <name evidence="3" type="ORF">PGTUg99_017626</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000408">
        <property type="protein sequence ID" value="KAA1088006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1T3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1088006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="138" mass="14783" checksum="52A9DE3298BE289D" modified="2019-11-13" version="1">MSVNPTSTSRAIASTSSTSTSSSNHVSHHAPTSTRTDNRHSSLAPSSNPIISLPPRSTAPKSRHISSEVEVMPTSNEFVPLTSTYDPRQEDGFHIHSPTPFDSTTETDQLDRLPTPLDLMTPAPVAHHVGSFATVAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2X5</accession>
    <name>A0A5B0N2X5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2X5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="222" mass="26198" checksum="38211B341B687D59" modified="2019-11-13" version="1">MNLEVFHDQSRENSQDSTQYHGMNPKLNAPEEIKREIISLIWILNEKFNSKNQGLEVSEISYAIFDYLDFIERHYFPEIIKETSYNQFKSFDQFQEQKDTIILLSKTLESFAMEKCILNLFSSHSDHQIYHNYPDDLESLLEKHHKKSISTFSKFCEAYKKIERNNLLSDWLHIGGPAGDLKYTFEIGQSNIEAMDKLKTEITISRKKNLENDTSKKRPKVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5Z8</accession>
    <name>A0A5B0N5Z8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011946</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5Z8"/>
    <dbReference type="OrthoDB" id="2509318at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="343" mass="39343" checksum="AF3318AB51FC7CDB" modified="2019-11-13" version="1">MTMTDRSVSHHDDDTIEEVCDKLQSQLKLDPQHLKIALITSKVNPSTWSCSSPISHFQCSICFHQKFVPESGTAAVIFANAAYHQLDGPNTGIATQRTANFVFDQSFRVTIKQNQQDFVRKTARIILLLPTLEAYSNNPHKNGALPKTLFYQTLPDAWKEEHLQPSSNQDIPQSPSAYREAVRDLLKYQRSNLRVLLLRNILETKRIFITDHVPNRKAMLTSIYEDLPPKGGKMTTAKITHQVTTNYAMRARMCYARLVMVYFYVHKSKKDLQWSDIDERLAVLRGSSCEFQQHHSILVLNKDFLLFSQKRNYKSMKPKEFSVPTLEEVQDLVDTGTIPATLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7Z5</accession>
    <name>A0A5B0N7Z5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7Z5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022678741" evidence="1">
        <location>
            <begin position="23"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="76" mass="8502" checksum="B403AA6A39AC5EEE" modified="2019-11-13" version="1">MQLPSAITLTALLASFAMSTRAVYPPPNDYIMSMGLNWSNGYQSAYNSKGEDTFRWFKDYDKPRKGISTSRLATGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N889</accession>
    <name>A0A5B0N889_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014334</name>
        <name evidence="2" type="ORF">PGTUg99_003042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000528">
        <property type="protein sequence ID" value="KAA1063740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N889"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137401" description="Secreted protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1063740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14159" checksum="FB6CCE1EBAB5E705" modified="2019-11-13" version="1">MVAGIAVLVVVMVVEKAERWTLCQAHPIDAFAWSTAEHIFMNLQISILRRHRQTSTPLRPTLHLPDSASIHPMFGARLGSHAEHGGILVPKGARGRYPRTHSPGQALAFVGARGCRFDPGCVHAEDQGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAQ4</accession>
    <name>A0A5B0NAQ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcription initiation factor IIA small chain (TFIIA 13.5 kDa subunit)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TOA2_2</name>
        <name evidence="5" type="ORF">PGT21_019900</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NAQ4"/>
    <dbReference type="OrthoDB" id="586585at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="4">
        <location>
            <begin position="107"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1085793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="160" mass="17791" checksum="4445CF4E2FE68B1A" modified="2019-11-13" version="1">MFEKLENCTASTQHLQGWWLVGSTNSGTKHRKVVLCKKNLVTVGFEPTHLAIEDLKTAALDQLGQMTMESPGKNEYMFRRPPNDRPIFDKSASQVLSTQLRSKCTVKGHLSTYRLCDEVWTFLLHDSTFKLEGGDSVGPVKRVKIVACKGNPNAGPAQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NH51</accession>
    <name>A0A5B0NH51_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000103">
        <property type="protein sequence ID" value="KAA1088557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NH51"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1088557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="131" mass="14595" checksum="28C79E34E10FD785" modified="2019-11-13" version="1">MTVGVGPRRKEHGCDDPTVHADEVYMWRADDSVIPTVGRPTEPRRPAVNNLTTGGYPEELKVLWWCNYTAGRIEKSRSATKAQPEFADTMAPRTPVKYHGFAPEAGRLKSIFRGDSRFQMVPRAEPGRPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHQ8</accession>
    <name>A0A5B0NHQ8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rnh202 triple barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030494</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHQ8"/>
    <dbReference type="OrthoDB" id="29098at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.530:FF:000008">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040456">
        <property type="entry name" value="RNase_H2_suB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041195">
        <property type="entry name" value="Rnh202_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383:SF11">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17745">
        <property type="entry name" value="Ydr279_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rnh202 triple barrel" evidence="2">
        <location>
            <begin position="8"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="335" mass="38106" checksum="D1DB6FD48B9E2543" modified="2019-11-13" version="1">MAASDPIILPTCVLEVKDDLPIVRLPHPRTRLPALFAIKAGQGIWEIQSIVSSPTSSRSWFFRPRREDQIDQLISQQAGRLLVVTPFDPFFLLLGLFLGHANSGNETDLFAIDKKSTDHRARFEDIETILERIQEHWLFAGSAEGPTAGDVELFTNEAFQDKHLSRIFAPLHQEETGTTLWRLEAERIVKEIRRKVDQLNSVGLDAGRDVRTVWTRLGSKEGLFDFEQTSQNLPIINDIYKKISLEMIQAYLPKPISEHLQGMNEYQFLALRKEKENQARRSTINPLEIGRNPQGSEAKSKSTAKPPPAKRKKTEDSLKPRPKGTLHQFFKPKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIA3</accession>
    <name>A0A5B0NIA3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">40S ribosomal protein S22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPS22_2</name>
        <name evidence="5" type="ORF">PGT21_034530</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIA3"/>
    <dbReference type="OrthoDB" id="10250260at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1087609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="198" mass="21491" checksum="6D2E5EEFD03343E7" modified="2019-11-13" version="1">MDGDGQAIPGELASTGFLEPAGGLTVADPVSRLTATAARLKLTDPPSRHTTDTTRPKDEAKVHAPLIAHALCSSMASTNHDFHPPVLLLKDRRGIFVVQAHSNSSQDACVGSGYIDEFEYIDDHRSGKIVVQLNGRINKCGVVSPRFNVPLDQIENWVDRLMPARSFGIIILTTSAGIMDHEEARRKHVAGKILGYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJI1</accession>
    <name>A0A5B0NJI1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018681</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJI1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="117" mass="12948" checksum="CE527E68BC9A026E" modified="2019-11-13" version="1">MGLLCMRVLSRFTSTTGSTSEHTSVGSQKTVGMGRLPSWHSTVSTKISMTSRLHSSISSTKKTNSGIGFKKTARRPQTIQYNQNNFCVYLKYWKRVAQQACEEKLKAPTSVEGSPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLH4</accession>
    <name>A0A5B0NLH4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Splicing factor 3B subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SF3B1_1</name>
        <name evidence="13" type="ORF">PGT21_015552</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLH4"/>
    <dbReference type="OrthoDB" id="438939at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000810">
        <property type="entry name" value="Splicing factor 3B subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000088">
        <property type="entry name" value="Splicing factor 3b, subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000329">
        <property type="entry name" value="Splicing factor 3b, subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015016">
        <property type="entry name" value="SF3b_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038737">
        <property type="entry name" value="SF3b_su1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12097">
        <property type="entry name" value="SPLICING FACTOR 3B, SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08920">
        <property type="entry name" value="SF3b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Splicing factor 3B subunit 1" evidence="11">
        <location>
            <begin position="248"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="783"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="12">
        <location>
            <begin position="1020"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="110"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="222"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1089364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1240" mass="137833" checksum="26A087F38129B9CC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNL5</accession>
    <name>A0A5B0NNL5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hydrolase 76 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">DCW1_2</name>
        <name evidence="7" type="synonym">DCW1_6</name>
        <name evidence="6" type="ORF">PGT21_017695</name>
        <name evidence="7" type="ORF">PGTUg99_004462</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000177">
        <property type="protein sequence ID" value="KAA1125480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNL5"/>
    <dbReference type="OrthoDB" id="9984024at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Glyco_hydro_76"/>
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    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
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        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
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        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
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    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
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        <location>
            <begin position="19"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1090911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1125480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="126" mass="13922" checksum="FD710CEAF9BF9F13" modified="2019-11-13" version="1">MIGYNLLISFLGIYSVLGASYSPTLDISNTNQLVNAATSALKNLLTYYSGSDGSFDQADTPWHESGMIWGMFMDYAQYTGDAQFSGLVTSALVNSSFKTAQYVQNIENQSNAKSRLIEVLQRLPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPY8</accession>
    <name>A0A5B0NPY8_PUCGR</name>
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        <submittedName>
            <fullName evidence="4">Transcription factor TFIIIB component B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BDP1_1</name>
        <name evidence="5" type="synonym">BDP1_2</name>
        <name evidence="4" type="ORF">PGT21_019369</name>
        <name evidence="5" type="ORF">PGTUg99_017146</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Myb_dom"/>
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        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039467">
        <property type="entry name" value="TFIIIB_B''_Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929:SF0">
        <property type="entry name" value="TRANSCRIPTION FACTOR TFIIIB COMPONENT B'' HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myb_DNA-bind_7"/>
        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="691"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="691"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="832"/>
            <end position="889"/>
        </location>
    </feature>
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        <location>
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            <end position="40"/>
        </location>
    </feature>
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        <location>
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            <end position="115"/>
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    </feature>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
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        </location>
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        <location>
            <begin position="861"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1125540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="889" mass="99335" checksum="B96CA4F1DC236E16" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NRM1</accession>
    <name>A0A5B0NRM1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_030913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1091302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="233"/>
        </location>
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        <location>
            <begin position="438"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="461"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="618" mass="70147" checksum="ACC737B9C905933D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NS51</accession>
    <name>A0A5B0NS51_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">PGT21_034929</name>
        <name evidence="2" type="ORF">PGTUg99_036233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        </lineage>
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    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NS51"/>
    <dbReference type="OrthoDB" id="10291821at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="289" mass="31578" checksum="8630777C871FC8B7" modified="2019-11-13" version="1">MSLSPAEVNAATALADMLTNNSPLSSSYSTDRHVSVGLDDGFTLPPLVEIRPSAYLQAHAGAILDVNTSTNADLGFVPLFNAAPAMPVSLSSDQDGSIATEDELGPSDSEEDSRLQTLPDSSDRCRKTYLQSNATLIASPTPSEQFVRPTEALTSPGPKPIDYLVVIPASNGYRSPSVVSFSDLPGNGTADLPYYVNTPPPGSDAEENATDRGPFFAEVVRERTVRTARRRIKERFHDYFHILDSFPYCTAARTGERRRSARQWLYREVMDEMDLVLADVDEVTMQLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSI2</accession>
    <name>A0A5B0NSI2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_017039</name>
        <name evidence="3" type="ORF">PGTUg99_026256</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NSI2"/>
    <dbReference type="OrthoDB" id="992776at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100">
        <property type="entry name" value="IMP2'P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100:SF4">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="405"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="620"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="721" mass="79477" checksum="4A94429B11A6530F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTW8</accession>
    <name>A0A5B0NTW8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_050042</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTW8"/>
    <dbReference type="OrthoDB" id="5597284at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="28"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="319"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="210"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1092671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="357" mass="41397" checksum="7DC4FE2C9366A959" modified="2019-11-13" version="1">MKKSIKRWVQSCESCQKRSRILQKEEGKATYKATLFERVSMDAVHIKAGRWKYLIVARDDFSGWAETVGLTSLKSRNISEWFTAEWIYRYGVPKEVTVDGGAEFKKELQSAMKRVGTKLTIITPYYPEAQGMVERGHKEIKDALTKMCGEDGKKWKDYLPLVTFADRISTKRTTGYSPYELQFGQLAILPIDQDLGSFLTVDWDKVKTTAELIKARAEQLESREDKLDEAFERMRKTRETSVRYWDKRLAHRLRKPLNPGDLVLVYNKALETQWGKLFTNKWNGPYKIVEQINQGPYKLSELDGTILTRRYAASHIKRFYPRGESTEEEGEELESEEEEQPEEESTEEESIAVEATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUQ0</accession>
    <name>A0A5B0NUQ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035583</name>
        <name evidence="1" type="ORF">PGTUg99_036915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUQ0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="119" mass="13897" checksum="F86DF46313756D96" modified="2019-11-13" version="1">MAHAIYPHRTPGIPSKSPWRNCYPLAPGTRIHDTSHAVAGLHLLIRYNPRFDLERNTTFFLLEIDRADQPSNESTLALNRLTGRALRILQTLNGHKQRKRSTEQGRREFTNWMRRKKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZJ6</accession>
    <name>A0A5B0NZJ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010937</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZJ6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="343"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="815"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="851" mass="95816" checksum="C3169ABF178941AD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P098</accession>
    <name>A0A5B0P098_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033460</name>
        <name evidence="2" type="ORF">PGTUg99_013278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P098"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="556"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="631"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1134" mass="128924" checksum="9981ABAB2438B176" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7L1</accession>
    <name>A0A5B0P7L1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010257</name>
        <name evidence="3" type="ORF">PGTUg99_023176</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000343">
        <property type="protein sequence ID" value="KAA1099121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7L1"/>
    <dbReference type="OrthoDB" id="2501310at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137667" evidence="1">
        <location>
            <begin position="21"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="610" mass="69492" checksum="49FE47C6B183E404" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7U6</accession>
    <name>A0A5B0P7U6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_024783</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7U6"/>
    <dbReference type="OrthoDB" id="2507066at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="53"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="254"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="618"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1096674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="657" mass="74512" checksum="FB606D558DDBE091" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8K3</accession>
    <name>A0A5B0P8K3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032322</name>
        <name evidence="4" type="ORF">PGTUg99_006682</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8K3"/>
    <dbReference type="OrthoDB" id="9995306at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019519">
        <property type="entry name" value="Elp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018627">
        <property type="entry name" value="ELP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184:SF6">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10483">
        <property type="entry name" value="Elong_Iki1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1107907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="225" mass="25739" checksum="83253A17C11592CB" modified="2019-11-13" version="1">MNFPLEHYSIPAKLSLLLVDSIDSPANFILLQLISNALRNHRAVILLLTDHDQNYWLSLLNKNRVNNQLLTIIQPKLDEDIKLLLTQQLTKSNNNPLVIINNLSSIIWNNNLPTHQLTQAFKALLPSQLNHDFSLVYLFHNDLIKNNNRDREIFIQLINSSDIILRTRALGGQAQTQGELIIQRGPRYLADLNLPITTSSDRTVQFKIQDHSVSFHPKGLDKAFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAG2</accession>
    <name>A0A5B0PAG2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036606</name>
        <name evidence="3" type="ORF">PGTUg99_027346</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAG2"/>
    <dbReference type="OrthoDB" id="2496543at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="436" mass="49496" checksum="03846E9944734F37" modified="2019-11-13" version="1">MSDSNSETGNSSTSDDESGASQTSETGPKSKTDLVVEALDDMDRKYRFESKREADVDKNLTINDMGEKLKRLDELETSLLPSMEELIDQLLVALDIKGSSDYPGPQPDLDLTLEILSKLDKNLKRTVSSGVFLALESPLPDAEHDHHLDKAKIFRCTLLLHEIECLVVNQIYDLLVCCMSVVQWCESLSDCSEEALFQSEVLLSRREALMSATQCQHEIDRLIKFSQKSDLAILQMTWLPPAKLLNRVLVNFTHLTNPRFDSNPEPKRNFWIVEDRNQERAAPREEIAQVAKSTIPIIKLARILLGKLSRLSRKEVPFTLHSAIDSKSVNKLKSAQHAIAVQLDRIGLTLLKDHRTNEALEPIFNQVHTVVKQISWIVEFHLVDIAFHLVPLPHLLLNGVNSPTRHFRAQFCPWKQLWDVAVSRLLDFLCSIETEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PB45</accession>
    <name>A0A5B0PB45_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022130</name>
        <name evidence="3" type="ORF">PGTUg99_000823</name>
        <name evidence="4" type="ORF">PGTUg99_018647</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000383">
        <property type="protein sequence ID" value="KAA1091715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000346">
        <property type="protein sequence ID" value="KAA1098795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PB45"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366385" evidence="1">
        <location>
            <begin position="19"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1098795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="82" mass="8520" checksum="5BC0108A5B032C2A" modified="2019-11-13" version="1">MKITMLFAALSAASGAFAAPAQAAAAAKDLSIGAGVGIGIGAGVGPYGYPYGAYPGWGGYNYYPYGGYPYGDYPYGYQYYQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBI7</accession>
    <name>A0A5B0PBI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_026005</name>
        <name evidence="9" type="ORF">PGTUg99_034790</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBI7"/>
    <dbReference type="OrthoDB" id="70376at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="175"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="340"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="351"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1097984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1117049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="380" mass="42386" checksum="F64E9DEC99F60759" modified="2019-11-13" version="1">MNSRQANKTVPSPSKTRSIHDQTNHHLEPAHPSSSNPDRWKTQRRKESFVAQVERIRENNLKRREGIYTKTWQELQETQNALMSNPPTEINYLVRLHRESLKREQELVAVRVYHDHLISATRAAFDAEVRSIEEEFTNARTQAKEKLLESLEERRKKLKDEKELIDFNEEAVGHSHRTLTTRGLRNRSSNRTGFPRGSPALGGSSYPTTGSRLEGSSFNNPDHLAADPELGSSATGLPPAAGFITADDPFSMATLNVDPHLRQSPIFQQLLQLGTHSGSRRGGPGAPPGGRRAPTGLPGAISLPSITPGTWNNFHKSQLGICSAKNDDTDADVEQIRHLAGLLSPKKRRMNNPSNPSNLNQSTTNPNSHTLHHHREKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBS7</accession>
    <name>A0A5B0PBS7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Lariat debranching enzyme C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_027745</name>
        <name evidence="16" type="ORF">PGTUg99_032534</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="16 18">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the lariat debranching enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBS7"/>
    <dbReference type="OrthoDB" id="407609at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008419">
        <property type="term" value="F:RNA lariat debranching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00844">
        <property type="entry name" value="MPP_Dbr1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000035">
        <property type="entry name" value="Lariat debranching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007708">
        <property type="entry name" value="DBR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041816">
        <property type="entry name" value="Dbr1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849:SF0">
        <property type="entry name" value="LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849">
        <property type="entry name" value="RNA LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05011">
        <property type="entry name" value="DBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01124">
        <property type="entry name" value="DBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lariat debranching enzyme C-terminal" evidence="14">
        <location>
            <begin position="399"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="277"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="435"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="435"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1098064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1116971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="587" mass="65396" checksum="D7D52BF853B15663" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PC26</accession>
    <name>A0A5B0PC26_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoglycerate mutase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PC26"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000033">
        <property type="entry name" value="Chromosome 12, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF42">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="534" mass="58471" checksum="ECA0342BE68BAD79" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCB9</accession>
    <name>A0A5B0PCB9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031741</name>
        <name evidence="4" type="ORF">PGTUg99_017570</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCB9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137743" evidence="2">
        <location>
            <begin position="25"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1116775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="271" mass="24584" checksum="7E6043134130D1AE" modified="2019-11-13" version="1">MQVSLLAALLVATSGLFAVSSVAGQRHSSDYGSPNYDSDSYDPPESYSPPESYSPPESYSSPQHKSYGGRPSRPPSQQYPPPAYHPSPSYPPPGRPGQGPPFGGMAPGLGGIAPGIGGIAPGLGGIAPGIGGIAPGLGGMGNGFGVVPGFGGGLAGPYGMFPGFGAGVGANVAAGVGAGGPGGFGGPGVGVGANAGVGAGVGGPLGAGVGAGAHAGVGAGVGGPLGPGVPLGPGVGAGAHVGVGAGAGVGGPVRVPVGVGAGAGVHAGAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRY8</accession>
    <name>A0A5B0PRY8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_017592</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRY8"/>
    <dbReference type="OrthoDB" id="166907at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019519">
        <property type="entry name" value="Elp5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10483">
        <property type="entry name" value="Elong_Iki1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023043778" description="Elongator complex protein 5" evidence="10">
        <location>
            <begin position="19"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="387"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="389"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="417"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1103423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="431" mass="46698" checksum="54436D33768EA0D6" modified="2019-11-13" version="1">MKMAGALLLDSLLSRTIPARLLILQDCPAQSAHPIIHQLNTTILISTSSTPSTTTTTTTNFNLNQLTTEQYRNTILNAISIQKASPVSIVLNSIQELCQLISDHLAFKFLRNILELLPNPESRLIVVDSPHLQISSSNKSVDCSTALRSSSLSTSYIHLTLHPTSLLDHLINVYGLPVPMPSRQPVGTHYDVRLFGLLAELAGECEPYLEPIGGGKDGLQVVPQALLVPPSLAESCSPNILGTCIVQWAAKNVPLAPSIPSASIALKPATLKKNARIGRPSAHLAAIQIGLEALKLVPTPLGFTLHPLPVSKVLVKRTFKLFTGPINQSTPTSDTHQHQLTFNLALTDKQRAEREAVALPFLPRRAEDGTVIEAPGQMFDGSFQNFLPSSNINHHSNNTHNNPNNGAKSGMILYEPDSADDLDDEEPDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTD9</accession>
    <name>A0A5B0PTD9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009572</name>
        <name evidence="2" type="ORF">PGTUg99_001939</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000316">
        <property type="protein sequence ID" value="KAA1103768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTD9"/>
    <dbReference type="OrthoDB" id="2508627at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="301" mass="35084" checksum="DD648577A5477691" modified="2019-11-13" version="1">MFILLEQRLLARSLPEFLSLRSKTSQSKPEMLLWASVLVFNYVALLIVLTENPAECSVARLLKRMDTAIESGRLMESEPALAHTGQHFDDAKALQSGGLDLKNSPPVHQEATFEKTITPEKEQELLNKAKAKSRVLSRMFGINEKEMVPKLYNVLRNHHLGLLADEKYQTAALMDVEKLKMRPWKYKAFKKFINSGNHDKFYKDVAKERVMIKIEEAIDFLHPDHTKTEMKFFKKFLAGRNLRYKDPEQFTKRVEQLWVQWKESKHSKSFSFKVTAFGERVLRHVKKICNKVLGRYKKNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUA8</accession>
    <name>A0A5B0PUA8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_015563</name>
        <name evidence="5" type="ORF">PGTUg99_036250</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018808">
        <property type="entry name" value="Muniscin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF54">
        <property type="entry name" value="SUPPRESSOR OF YEAST PROFILIN DELETION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10291">
        <property type="entry name" value="muHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="4">
        <location>
            <begin position="11"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="61"/>
            <end position="93"/>
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            <begin position="154"/>
            <end position="180"/>
        </location>
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            <begin position="291"/>
            <end position="485"/>
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        <location>
            <begin position="497"/>
            <end position="646"/>
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        <location>
            <begin position="659"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="727"/>
            <end position="854"/>
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            <begin position="916"/>
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        <location>
            <begin position="1249"/>
            <end position="1313"/>
        </location>
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        <location>
            <begin position="73"/>
            <end position="86"/>
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        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
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            <begin position="323"/>
            <end position="334"/>
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            <begin position="341"/>
            <end position="356"/>
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        <location>
            <begin position="378"/>
            <end position="392"/>
        </location>
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        <location>
            <begin position="420"/>
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            <begin position="442"/>
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            <end position="1313"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1068697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1104230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1378" mass="152155" checksum="FE28B33FF5A6DB5E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYI1</accession>
    <name>A0A5B0PYI1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023532</name>
        <name evidence="4" type="ORF">PGTUg99_027829</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
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        <location>
            <begin position="45"/>
            <end position="132"/>
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            <end position="63"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1121028.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="594" mass="67661" checksum="082A22F3F7D0AE43" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0PZR2_PUCGR</name>
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        <alternativeName>
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        </alternativeName>
    </protein>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
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    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZR2"/>
    <dbReference type="OrthoDB" id="2501965at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="9">
        <location>
            <begin position="217"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="436"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="657"/>
            <end position="683"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="745"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="452"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
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            <begin position="498"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="772"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1106327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1109383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="878" mass="96125" checksum="05974E3C272B498E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q353</accession>
    <name>A0A5B0Q353_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q353"/>
    <dbReference type="OrthoDB" id="2507825at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="442" mass="48265" checksum="F20AEFCB22898509" modified="2019-11-13" version="1">MNMHHLNGQTETDSADYQYQQHQHPAPPTPASSWPPNQTYPIAEQVNYPSASYPIPPTTHYAQAYQQQYFGQLPAHQPPIIGLLPLTHQQLPMLIPANHAPAAPVAPVASLPCTAPDDLRKKRKARRTPEEIARAEDAAAAAQAEKLKKASNKAMALREKAASKRERDMVKQAELAAKESSNKQKWTDNSSLELLAMIKRVKEEYLDLKVKTGGFMKFTQFFGTRKHYKKHYGLLVDVTAKAMWSRYQVIMVAYRKVKDHVDQSGSGGIHDALAQFHLALSLWEFLVDMHFGNWAANGTGQGELAEMSDEFGTKAEDVPGSDSEADGLSGSKLPATLNVSEIPAAPGIKSNTRSSKCACLSAGLTAAKRDLDSSSEDESELPRKMATPKATSAKKPVGSTPSGNGRPPAEGTLPIPRRCGRVEEAPKKEDSNGNAMVLLMHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5M5</accession>
    <name>A0A5B0Q5M5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SGS1_40</name>
        <name evidence="8" type="ORF">PGT21_013397</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5M5"/>
    <dbReference type="OrthoDB" id="2503578at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="112"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="456"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="542"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="468"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="507"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1108458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="561" mass="61789" checksum="342EC5A9652C7D06" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5P9</accession>
    <name>A0A5B0Q5P9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014164</name>
        <name evidence="3" type="ORF">PGTUg99_029213</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000002">
        <property type="protein sequence ID" value="KAA1138564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5P9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366515" evidence="1">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1138564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="52" mass="5491" checksum="53768DECB85936FC" modified="2019-11-13" version="1">MLVRLAFLGFFLLQLQAALGAPAGSVTRRSNDDSGDVDGGRDCDLLNNCPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6D4</accession>
    <name>A0A5B0Q6D4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospholipase/carboxylesterase/thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_025779</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6D4"/>
    <dbReference type="OrthoDB" id="437457at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF67">
        <property type="entry name" value="PHOSPHOLIPASE_CARBOXYLESTERASE SUPERFAMILY (AFU_ORTHOLOGUE AFUA_5G09340)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="3">
        <location>
            <begin position="42"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1108796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="240" mass="26606" checksum="48AEF6F0F2A7678F" modified="2019-11-13" version="1">MNLHLHPPTASRSAATKPAPSFPQLSPAPVFDYHPSADGIDSNLLIFFHGLGDTQIPFSQLARKLKLPQTACMSIRAFERVPFLEEEAYQWWESFDITGSPVSHPNPSSTLIKLIGMLDRLTTDEIGWTPEDIHLFGFAQGASCLSELALLWTRSRSTAAPLASIVSVSGPLLSLPTIPTSSRSPTPVFLWTRRGEDDTGKWRAGFERGFRSVQHFLAPRASQEQMPMTPQEWKPILELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAV1</accession>
    <name>A0A5B0QAV1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAV1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="135" mass="15537" checksum="B82D23FFF54D4D24" modified="2019-11-13" version="1">MLLHFLFYSHCKYLEICNSLNTASSIFDPRPSMTGLRMNNSLQLCSRFFPHQSVFTRYVCSFSECFLYCSLLNMVLLSGLFSLCPAVKEGVSSSFISFFAYGSLLDLFLSRSSCVVIACYFFFTYYFKSFIKMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAZ6</accession>
    <name>A0A5B0QAZ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="2">6.3.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_011070</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAZ6"/>
    <dbReference type="OrthoDB" id="3226056at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like C-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1110113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="155" mass="16961" checksum="4625C73C0AB04C64" modified="2019-11-13" version="1">MSGLGYESPCPWNPDQTLGVALLEPTRLYTQQLLPILKSEERLVKGLSHITGGGFLENVPRVLPAGLGCEIDAGSFPLPAPFKWAMNQCNIAPLEMCRTFNCGIGMVLIVAKESVSKVISLLEAHDPKRTTAIHQIGRVTHQPGVEMKRLESWLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBK3</accession>
    <name>A0A5B0QBK3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Adenosine kinase</fullName>
            <shortName evidence="11">AK</shortName>
            <ecNumber evidence="4 11">2.7.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Adenosine 5'-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ADO1_2</name>
        <name evidence="13" type="ORF">PGT21_023298</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine + ATP = AMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.20" evidence="4 11"/>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBK3"/>
    <dbReference type="OrthoDB" id="432447at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00659"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000014">
        <property type="entry name" value="ADO1p Adenosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001805">
        <property type="entry name" value="Adenokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769:SF3">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00989">
        <property type="entry name" value="ADENOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="12">
        <location>
            <begin position="25"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1110499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="356" mass="37836" checksum="5668AA1148D5AE42" modified="2019-11-13" version="1">MSASTKLLAMGNPLLDMQISTDEKMLEKYGLKANDAVLVNDSQKGIYAEVASLSPVYVAGGAAQNAARCAQYILPENSTVYLGAVGDDDLANQLREANKKAGLKELYQVVKEFPTGACACLITGHHRSLCTQLGAAEKFSPSHLKTEPVVKAIQDAQIYYLGGFFLTHGIESSLALAQAATESQKIFTMNLSAPFIAEFFKDNVDQLLPHVDYLFGNESEAAAYAAAHNWDTKDLPTIAVRIAALPKKVQTRPRVVIITQGSESTIVASTNASAFSSPADLKAVEAGHVLIVPVSPLKDEEIVDTNGAGDAFAGGVLAGLVLGKPIDQCIEIGHKLGKMCVGQVGPQLKWPKVQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QD65</accession>
    <name>A0A5B0QD65_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chromatin-remodeling complex subunit ies6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">IES6_2</name>
        <name evidence="7" type="ORF">PGT21_036988</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QD65"/>
    <dbReference type="OrthoDB" id="49520at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029525">
        <property type="entry name" value="INO80C/Ies6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="6">
        <location>
            <begin position="251"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="99"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="176"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1111170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="302" mass="33812" checksum="B3A49E5A12B46E77" modified="2019-11-13" version="1">MQAGTFRKRLTERTNQRASSHRAKDGLRWAVWSLLAPAAHSMYDDGAQRNLLSFIHQPSIERSANCRRHIGPLEPGCSYSVQQPAISFLKPSHIFDRTTKKCQAPPKGSSRNSSWFKAGAGGSNEPPPNATSLADHLSYSMIAKPFKDDKIKKGPRRNKPLKQILAAERLRAKHVSDVLLKNNPTPDEDVEMTAAPDEQQPNSNDQQQQSQSQPAVNKKARREILGPVNPDVNYNTYMIIEAPPSVIPPKKYCDITGLEAPYIDPKSRLRYHNAEVYELIRTFGPGLDQSYLSLRGAHITLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QE33</accession>
    <name>A0A5B0QE33_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000022">
        <property type="protein sequence ID" value="KAA1111244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QE33"/>
    <dbReference type="OrthoDB" id="10448694at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="14906" checksum="BD669A2D26B4C9FC" modified="2019-11-13" version="1">MTRHSPHLKNTISITLQACLQPFVSVIYHPISLFKLQRSSSLQFGTTTQMFSLKFRHFTSQISPNSPFAGPPPTLSASLRQCRTNLKSHQLSALSFLLRNEHPNNNTKDLWYHDDTPGFRHYFDKRFCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHD8</accession>
    <name>A0A5B0QHD8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_002798</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHD8"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023037785" description="RING-type domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="107"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1112582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="182" mass="20380" checksum="D97FFF645F32577D" modified="2019-11-13" version="1">MRLHTLASLGIFAQLLLGVQGLLSARKDLKGTRKICKFIKGSFRPKPSGENPQEELKMDTVIYRRTGPIEDTVFRPESRGKQLRLEEPEGLGETGEKNEGGSKQAQCIICPNGFSTEDTVVKLAPCHHYFHEGCIMDHLSMDCKTVKVDKPCPFCKKRIGSILYVKSKRGQLEIVHPSQQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHQ1</accession>
    <name>A0A5B0QHQ1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGT21_007972</name>
        <name evidence="16" type="ORF">PGTUg99_020447</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="16 19">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000407">
        <property type="protein sequence ID" value="KAA1088150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHQ1"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000085">
        <property type="entry name" value="Aspartic-type endopeptidase (CtsD), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000060">
        <property type="entry name" value="Aspartic-type endopeptidase ctsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF75">
        <property type="entry name" value="ENDOPEPTIDASE (CTSD), PUTATIVE (AFU_ORTHOLOGUE AFUA_4G07040)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474588" description="Peptidase A1 domain-containing protein" evidence="14">
        <location>
            <begin position="25"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="15">
        <location>
            <begin position="307"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="512"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="547"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1088150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1112738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="643" mass="69735" checksum="100960DDA48BE1D8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJB0</accession>
    <name>A0A5B0QJB0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJB0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="133" mass="14825" checksum="386A724E86FFE957" modified="2019-11-13" version="1">MLSSSVVDICRFLDNNFSKCSAKNFRSLSLIKSFFSAIESFFSPIESFSSPIESFVSPIESFVPPIEFFSSPIESFVSPIESFVPPIESFVSPIKYFSTSIVSFVLPIERSLSPPKSAFFPLSTIDLMLSLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QLB9</accession>
    <name>A0A5B0QLB9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022821</name>
        <name evidence="3" type="ORF">PGTUg99_005676</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QLB9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="62" mass="6361" checksum="EB310650B68ED121" modified="2019-11-13" version="1">MGGYSLGYPDIRQDSRGKGASAPESAPAGGYPLALAGIRQRIADIRNGLSATISTCDPLQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QLY5</accession>
    <name>A0A5B0QLY5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QLY5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="156" mass="18203" checksum="378D334DF5B5443C" modified="2019-11-13" version="1">MTQKRIRIHRSLESHQRKLTQERNRNIHNRRPAATVNEPLAPIQEESHDSESVHAPIEDKMEDPTNGDDGLQEENENLARQLRRALDLISRMNPNDFFDPTEARGADGLNAYDRMDWLFEEMDAEQMAEAEGDEEDAEPDQPQEDEWYPFKNKMAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNF9</accession>
    <name>A0A5B0QNF9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6278596at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021773">
        <property type="entry name" value="TPC11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374">
        <property type="entry name" value="FOIE GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11817">
        <property type="entry name" value="Foie-gras_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex subunit 11" evidence="2">
        <location>
            <begin position="353"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="959"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="959"/>
            <end position="970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1341" mass="151596" checksum="92E5B2213BEDA627" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPT3</accession>
    <name>A0A5B0QPT3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SWI/SNF chromatin-remodeling complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SNF5_4</name>
        <name evidence="2" type="ORF">PGT21_032653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPT3"/>
    <dbReference type="OrthoDB" id="10541654at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="106" mass="11904" checksum="CADC48B25522E01F" modified="2019-11-13" version="1">MTHCDTWMTRDDLGIAPWNDAWSPRKTVDGQELVRPAAVVTRGIHRKNRCPPTSNCARGVHKPVSPTSAIKEQLEEYKLHKNFEGHLASQTSLPKEEETIGGDMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQP7</accession>
    <name>A0A5B0QQP7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQP7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="149" mass="17106" checksum="D24997E32875A0AA" modified="2019-11-13" version="1">MPDTWLAMPMLSAGIGLTSLRIRFRISLGSWIPPCWTRLIIDPSLRLRNRIPPRWTRLVGSSIGLDPLSSVPCSGLKFQVSTHDYEGRYRSASEKTSRQPEEAESDRWLQSQLDSDRTTGPYLELDCTEYNLIWDRSEVPIKKKLLATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQT2</accession>
    <name>A0A5B0QQT2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Mitochondrial-processing peptidase subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MAS2_2</name>
        <name evidence="6" type="ORF">PGT21_037081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQT2"/>
    <dbReference type="OrthoDB" id="2504195at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF49">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="4">
        <location>
            <begin position="65"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="5">
        <location>
            <begin position="216"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1115568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="576" mass="63165" checksum="7B088348DA9778C7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QR33</accession>
    <name>A0A5B0QR33_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QR33"/>
    <dbReference type="OrthoDB" id="10468887at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="196" mass="22154" checksum="FCA8D43EC0DE036D" modified="2019-11-13" version="1">MNAFAHRLPLKDGAAVPDITVKQDVVVEQCYSIVRSHGETGWKDNHYAPGGSHAAYDPDTGTRKPELSRSNSVPNQNNHGNNRQQNHVNQYNNAGYGFSNHQERRRDSHRSCYGGNMMQGNFHNNYPNNYHYNTNYNNAHSQTHQQPSNFVNHYDNNNSGGYHQPMGDSRKRFRSSGNKSEANEKPPPSMARGSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QW17</accession>
    <name>A0A5B0QW17_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QW17"/>
    <dbReference type="OrthoDB" id="2551959at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="298" mass="33484" checksum="FB408D602DE3B307" modified="2019-11-13" version="1">MDAPLPGALPTPLHHCLGYPLNPDSTMDSQDQASLEGRAPPTEENERARFRELHRVDIDPNSFSAFVTLAGLEKVQEYHDRLEAVFRIEISNGRPKESCTALWEALVVSKHCSFNVFREALLLTEEKHPDQIKEFLRLAYFHQYPKHDSSNLSNKQMADAIKGVVANKPEKPRDSESVHPDWSVDIVKAGFQAEYKNSDLIVKPNLDLLRDMAGAWSQINYKAPFTSIVGPTMSGKTRLLLEFAKHVCVVCQDTDPIAATNATDKLLKQISDHIDAQEVIQTDPNQVQDTVQTPLMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWI4</accession>
    <name>A0A5B0QWI4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_050022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWI4"/>
    <dbReference type="OrthoDB" id="2506870at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="124" mass="13660" checksum="E2AB85DCF6F011FA" modified="2019-11-13" version="1">MRETRRGGQGRIVGIQDVVSTLNVQHDCHKGRCSIDLTKKKKLEREAIGRYVGEVTHTDNINYIVNLASLSSVDAHRNYSGVPVEAVDCRKQLRGVHEGLTQWHLAGTKTGPPEPPVVVDPALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXW0</accession>
    <name>A0A5B0QXW0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXW0"/>
    <dbReference type="OrthoDB" id="2503017at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="144" mass="16317" checksum="EAD4183F0FACEB88" modified="2019-11-13" version="1">MSEPKHQIPDLSKTNFGAWKQKVIGYCQQLGLRKYLTLKVAPTDAAALEVYQTNRSKTAGILVSNMGITNYNRFVTEKNDEDPQALWKLLTDYFEAKTPGNQARVYNDFCTFKYKGSDLTAYLELIDQHLKLMASVGIKMEGPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3D8</accession>
    <name>A0A5B0R3D8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3D8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="61" mass="6622" checksum="4DECB4449C5923C2" modified="2019-11-13" version="1">MVPPPSELRDSVSDLSAAKESGHQEKRICVAWACGNSLKAGPVQVHDYICHDASSALKRFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R482</accession>
    <name>A0A5B0R482_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_037146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R482"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="91" mass="10414" checksum="1B4C9ED6C7888782" modified="2019-11-13" version="1">MASSNSLHHHKTSDPSRIEAQRPSMPSSLKIKKFNPFSTPDARLHLSISIMNVTWIYLNFRPLTAVIQKRSQRGSFGSVNLLMRQFCLDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R791</accession>
    <name>A0A5B0R791_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029212</name>
        <name evidence="3" type="ORF">PGTUg99_032026</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R791"/>
    <dbReference type="OrthoDB" id="2510781at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="95" mass="10519" checksum="F4361F379A7BCA8C" modified="2019-11-13" version="1">MVAPHTPPVTSPLAHPGQRPSKPVRFGSINCRPKSSLVIRLSATFEEPFLTSSITHSYRFTDEGKMEMFSEEGKSDVQGRRVNYRDMMPAPGGPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RCP3</accession>
    <name>A0A5B0RCP3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005185</name>
        <name evidence="3" type="ORF">PGTUg99_017889</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000211">
        <property type="protein sequence ID" value="KAA1123162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RCP3"/>
    <dbReference type="OrthoDB" id="2502236at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="411" mass="43654" checksum="2AF23CAB9B0F9491" modified="2019-11-13" version="1">MAISTKFSSLRSSRRSAAPSSVIHQDQPPTQTTGSQPGALEPADLIRNNSMAATENTLGTKLEKKNRLKFFRRFSTRFASKPQEPKSDPIDPTLTATSDPMPNLVPLGKTNSNDPAMAPSDFTSNPCDFPAKAPFDVVPKTSASLSCDETGSLYRPKDAHGACAAETHCGLKSPSRASRSFWKRLSLSPSLQADPEVAGLATPEMSMSPVEDGNPTNVSSDGHPSSIMSSELSSAPSSANETADTSLASADEAESVEECGLAGIGLRDRRSICLHSLPIRNALGPSPLAGPANTKPLVHPAMRYAPSSSSPLSSRLAPPSSTVENHHPSTSSHLRTGPKRLSALSHTSSPVPSSLSQNKHRMSSAYLNLVPAQTYVDSETLMRILRGPSSYPEETLPRANSQRDSIYVPCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RLW7</accession>
    <name>A0A5B0RLW7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023088</name>
        <name evidence="3" type="ORF">PGTUg99_007287</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000175">
        <property type="protein sequence ID" value="KAA1125724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RLW7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="126" mass="14619" checksum="96DA2774B2FE51A2" modified="2019-11-13" version="1">MTDKLVDSSSNRRISSFSDFADGDLSSGSYEQQAKKNAWDLESKTKPPTLIQALSTDPIEIRDSIEKINHQFRNYRIQLEALKQRHAFIHPTFCAFVGDFSPLSVYLLRVYELEVERITLQDTRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RS89</accession>
    <name>A0A5B0RS89_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_028586</name>
        <name evidence="7" type="ORF">PGTUg99_015051</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000144">
        <property type="protein sequence ID" value="KAA1128138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RS89"/>
    <dbReference type="OrthoDB" id="1690618at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23953">
        <property type="entry name" value="zuotin_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.840">
        <property type="entry name" value="Ribosome-associated complex head domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032003">
        <property type="entry name" value="RAC_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042569">
        <property type="entry name" value="RAC_head_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044634">
        <property type="entry name" value="Zuotin/DnaJC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058871">
        <property type="entry name" value="Zuotin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16717">
        <property type="entry name" value="RAC_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26185">
        <property type="entry name" value="Zuotin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="107"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="253"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="253"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="299"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="333"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1100057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1128138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="462" mass="52438" checksum="DD768FD9D8DECCCD" modified="2019-11-13" version="1">MASETITLPISLDFQISVGYSKPSIGKLAAPETKTILPLGRAFQYHATRVLNNWTLAEHEVAIAKELEATKNDSAGDLGDEVDDGVGEEPESRYILELDPRDWKNQDQYAVLGLSSLRWKATPDQIKRAHRKKVLRHHPDKKAGQAGNANDDSFFKCISKAYETLSDPVKRRQFDSVDEEIDDGDIPSEKEVNASPEAFFKLYSPVFEREARFSLKQPVPSIGDINSTREEVEEFYDFWLKFDSWRSFEWKDKDANEGSDSRTEKRHIENKNRSERERRKKEDNTRRRNLVETALSLDPRMKRFKTEERLAREAKRKGPNASASTPATLSAEQIKEKEEAELKAKQELEKKNEESKSAKEAAKKAKEAAKKNLKKEKKTIQSIVTGSNYFLADGESVNASVIEGQLSELDLLFAALEPEEVSELRKEMESNHGQGKPAVKAVLIRFAKKVSDANKASFKQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S5N2</accession>
    <name>A0A5B0S5N2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023597</name>
        <name evidence="3" type="ORF">PGTUg99_012016</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000076">
        <property type="protein sequence ID" value="KAA1132779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S5N2"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF342">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="62"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="588" mass="66040" checksum="0838E9AEC726A21D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SI06</accession>
    <name>A0A5B0SI06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002311</name>
        <name evidence="2" type="ORF">PGTUg99_012443</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000011">
        <property type="protein sequence ID" value="KAA1137185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SI06"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1137185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="95" mass="10777" checksum="21043DCEC1CB959C" modified="2019-11-13" version="1">MESPLDEWRRVGLVISQGSRRVARTTASPEKKLFIPMIITRVESTDRKTVDEDIRQYTQCETTTGLETGQDGVDQAVADMRGLKQLSRKALRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKP0</accession>
    <name>A0A5B0LKP0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015899</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKP0"/>
    <dbReference type="OrthoDB" id="2502132at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="501" mass="57591" checksum="F4B7E5CAF05BBB7C" modified="2019-11-13" version="1">MSSSDLGKAIPEPVGPDRNPTFGTRGPESDREALLCKLACVQHSQPCSSCSCTTTTPYRTRPSPVITPEDITNQKLHVHVSGPRMPRITNTQDPMAITNIQEMSLEGQQEGTEGQPYTVKVMSPGDLDSFATALARLIDLVKRKINQFERKNRHPTWDDWGSNRVSPEFESKADQIKRILLPSIPGQLDRLCDMLSDPIYLQKLEAPQLQDGMKVLEELRSTVDQMSSSITAFWTPAHCRFFPNHERLVERSRGYRCERVQSKLTDVFFNLNALLLRYFDLLPLPEYKTITMDQAKLEYGELVGYMYLTEECGDHLIQWLDMSELWTIGEHWEEMADELAGLLEELVEFPSLHKDILTKPLHEFVPILKLSRLFFNKISEFTSDRSHPMSEMNTDQLLNLIQLTVSIPAKLTTFYDEIECKHQPLQIIDLTKVSELAKLFHAPLNLLTNYLSDQAANPNSPQDFHAKFIQWYTDWQYLFSLAVRRFHIAYRDAYSIFLTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLN4</accession>
    <name>A0A5B0LLN4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033682</name>
        <name evidence="2" type="ORF">PGTUg99_015798</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLN4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="67" mass="7411" checksum="051C5E0268D86715" modified="2019-11-13" version="1">MVNASQPVSPPHSYEGKPHLAPASQPDPSKRRFVCPALTAKTWCCIKFETLSGDNVYEYTRKACKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMK3</accession>
    <name>A0A5B0LMK3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004089</name>
        <name evidence="4" type="ORF">PGTUg99_020012</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1079995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMK3"/>
    <dbReference type="OrthoDB" id="76215at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF3">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="154"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1079995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="291" mass="32335" checksum="40FA911B0D1E8D90" modified="2019-11-13" version="1">MPPRVSKRPPCRPKRPSRPPTPPPHMSSTASQQSSALSVAAASNSSSLDHIPMSGLQIQSTEVRQDHSLPSISHMDWAASQSLPPLEYLQRGNFHPNLHSSYPPARSSVESIQTQLAPLNPRETPAPTSTANQPCSDSGDSETPRGTTRPKTLQWTSIMEKAAIQLYYDAALEGRRSDNGFKSDVHQHVVNKLNEQFPGAEFTISKCKSKLSQSFKKEYNAFLACCNASGFGWDEIRCEVTASNDVWEHFLKSHPQARRFRNQPFPEWEQLQVIFGHQGSFGGTPQGVPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMR6</accession>
    <name>A0A5B0LMR6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMR6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="105" mass="11923" checksum="BFD8410FB4EB254E" modified="2019-11-13" version="1">MVALNPYICADADVRSQFRQKHTSASAGGYPASEWAERGSRLFQETTSYSLNTRSFHPQPQPFQTLDLTISLDLVDFQSSIADHLVRLHLVRKDNRTFDLSSIDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LP43</accession>
    <name>A0A5B0LP43_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Yeast cell wall synthesis Kre9/Knh1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_013296</name>
        <name evidence="5" type="ORF">PGTUg99_006965</name>
    </gene>
    <organism evidence="5 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LP43"/>
    <dbReference type="OrthoDB" id="5420143at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052479">
        <property type="entry name" value="GPI-anchor_Adhesion_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35185">
        <property type="entry name" value="SERINE/THREONINE-RICH PROTEIN ADG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35185:SF1">
        <property type="entry name" value="UPF0619 GPI-ANCHORED MEMBRANE PROTEIN C1322.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033844825" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="4">
        <location>
            <begin position="24"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1065358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1090771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="199" mass="20539" checksum="143B03E8A89D477D" modified="2019-11-13" version="1">MQFYTALLGLVALSSGVTALSITSPSGSSYWVQFSTNTIAWSLSSGDPTSVSIQIINPTSKQFNGPFSIAEYVPAAKKSYDVTNVQLIIADGYEVQFVNPLNNTEVYATSAPFSVKTSGTAPAQVTYAPGTHESTNSSNNSTSNANASSKNSTMNADINGNSFKNSGNANTNAKSSSVRAAAMPSIVALALGSLCALVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LT04</accession>
    <name>A0A5B0LT04_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lipoyl synthase, mitochondrial</fullName>
            <ecNumber evidence="9">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Lipoate synthase</fullName>
            <shortName evidence="9">LS</shortName>
            <shortName evidence="9">Lip-syn</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Lipoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_010359</name>
        <name evidence="12" type="ORF">PGTUg99_024167</name>
    </gene>
    <organism evidence="12 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="12 15">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine + 4 H(+) = [[Fe-S] cluster scaffold protein] + N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 5'-deoxyadenosine + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="9"/>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1067003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LT04"/>
    <dbReference type="OrthoDB" id="3231at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000036">
        <property type="entry name" value="Lipoyl synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031691">
        <property type="entry name" value="LIAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004019">
        <property type="entry name" value="PRK05481.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009544">
        <property type="entry name" value="PRK12928.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="LIPOYL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949:SF0">
        <property type="entry name" value="LIPOYL SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16881">
        <property type="entry name" value="LIAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01058">
        <property type="entry name" value="lipoyl_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="183"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1067003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1083878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="435" mass="47753" checksum="1D12FD9DD4CE2D9B" modified="2019-11-13" version="1">MMIGWGSIGSISRPPWPTTARPSRPSERAVCCQSEFFQLSPSNPSLSLSSSPTSNRHTLIHHAMSSSRHLLKRVSILPQHLNLSSRFASSESSSSLNSVREAIKNDGLTFHDFVSAGAQDISVNSSDLVGSLTPTKRLPRFLKTDIPTSASFNSIKKDLRGLGLHTVCEEARCPNIGQCWGGDKGEATATIMLMGDTCTRACRFCAIKTSNRPPPLDPNEPERTAEAISKWGVGYIVMTSVDRDDLPDGGASHIAKTIQQVKAKAPHILLEALTPDFAGPNQRASTSCVANSGLDVFAHNMETVERLTPSVRDRRAHFHQSLDTLRWAKETGPAGLITKTSLMLGVGEEDDEVVMTLKELRKNSVDVVTFGQYMRPTKRHMKVESYITPAKFEYWKTVAEQMGFLYVASGPLVRSSFKANELLKSSLGKRLLKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LT58</accession>
    <name>A0A5B0LT58_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012413</name>
        <name evidence="3" type="ORF">PGTUg99_002862</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000324">
        <property type="protein sequence ID" value="KAA1103625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LT58"/>
    <dbReference type="OrthoDB" id="10389573at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="140" mass="15479" checksum="CA6BBC21735D5441" modified="2019-11-13" version="1">MYSCMDKQISELKAFYTARASGERLTVPDPVIDPSIAGASEDRLQGINSEIYEDAPNTAAWCRWEDINNKPDVHAGQEESDGSIERLEFSSEASSHSFRSGYASGSNLSEYDSNNKADDEEWDVPNNQGDTGAYEHNGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUH6</accession>
    <name>A0A5B0LUH6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUH6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="339" mass="36449" checksum="109BFC6B027EA5EC" modified="2019-11-13" version="1">MSSGKSSPTRPMYFVGPYDIIEVLGNDVPPGKSFGMFHYFSSIVVTDPKAGETVIVKSTKEGGYQCFFESNLSLYIGGTAQYRKTDPVHPDVDLGSELLGKVFVFGFGTIIKTERVASVGLNNTSQTDLHVTMRHYDYHNTKKSSNEFFTVYIVPGNPVLGNTFGFFTVDIEALMIGNITGFNEALGSWEVQVYLFSLSNGNSSFKAPRTPSSSGKTTQLRPGLRMIGGISATTPTSVSKVPSAEMTTPSSSAGRPFSPNIVPTPTPFTSRLSPGADNLSEGKIRERGSRPGGDEDEGRFVNTYKNGKRYPTSSSNTTPSASSGSVSLSDAQKRAKTCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWD4</accession>
    <name>A0A5B0LWD4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWD4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022819999" evidence="2">
        <location>
            <begin position="30"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="359" mass="40185" checksum="D8A983754ACA0AD3" modified="2019-11-13" version="1">MYPAKLFRIRKPSTVWILWAVMPFLRTTSSNMAATHEALPGLSQAHGNEVSCDERTPLSALAFLDLDSNSEQSQQFDDHQKIQKPHSILNPATGQGSSPLAFFQNHHQLSYDQNRSQVWPSALVQPHDILVPSRSLGDEYEYGQCDHLKWPEHKNSARRTKQRTTRENISHGKSDIVLSHTSWLPDNDHQNPQQKVQMTSVFEAATDLHLSKPINIDEHSKLAINQNNGGLPYVSAAPEKSDLQPLKSYFPEIADSRGFMSVYEGSPELYMKSSRGSIMSTGEKFDLPSHQIQNNKSEPTESINTAASLNSESGSSSKGYSLKKSKLMEERFQMAQPSIQSKYLSPKKGHLNIASRQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXV3</accession>
    <name>A0A5B0LXV3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025900</name>
        <name evidence="2" type="ORF">PGTUg99_018001</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXV3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="92" mass="10214" checksum="FDED423B4ECC5861" modified="2019-11-13" version="1">MLDYSDQLVRYGLLKVKLQQLQTLETDLQSQLQPSTSSQQPAKPLSGREESLSAGRNPSRPVRVYTLSGREESLSAGESVLFDRPRGIPLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYQ0</accession>
    <name>A0A5B0LYQ0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYQ0"/>
    <dbReference type="OrthoDB" id="2504766at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="1">
        <location>
            <begin position="140"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="347" mass="38981" checksum="BA7B00BE795EA5F1" modified="2019-11-13" version="1">MVAFGQNRRHNAFQLNNAVRFYACGVSDRVHEYLNYLGLSSSLKTAISALKSLARESARDFRRAMTIQDGLIAPKMCIDNIDIEQRVHQQSIGTQMKTLRGTWGYIHLPNQKLLETLDTAELDLAAFKLALNKVDSIIIEPHMFLPTPDEEQHEIQVWKSQIACVLQKYIAMPADKASAVSTDPPVIEQIAHGAPDLLMMKLMEASDNLAEGVGQVFQSLINQSGLDVEEFFTRLQPMDGDLATVQNFNFLQSQQGPSKGPHNALDNIFFQLGASHTLWNVGSTIFSHHFGDTSNSTNCGAWQHLEALGFPSEKAIQKKDFTLMINQMERVFEANIYYCLRYVLFPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZM6</accession>
    <name>A0A5B0LZM6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZM6"/>
    <dbReference type="OrthoDB" id="2510364at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="359" mass="40729" checksum="F4272C3B0A620EB3" modified="2019-11-13" version="1">MVLVTTLQRRSFQFYLAWCFASVLISCPMMIDLNKDPEEWYDEASSPITLHAIPPEIPPKKQKQPPVQLSSPSPSRSRARLSYDHGGLSSKDSIVARLTGESGLDTNHSGDHQLTNVDVIGSGISESNTGSNLDHVSQHSQSPRRGSKPADHPVDGVWDLPVVVQKDQKEIEFAYLITRGIIVEELLSKFSRRFHAKVESEDCQSRHWGSVDHHPRWSCARLSVAGANRYKVAKVVKHNSASIQPGWRLYSLYNLLAMSLYKLHGEFLTRINIPVLHHRNQQNKLFEWLEREIFTPGYSHPLLGIAPQSWLTWESGGPKLKHYGKIQVELVEYFSEKRRDPINSASRVLDIYQAQHASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M004</accession>
    <name>A0A5B0M004_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_000213</name>
        <name evidence="2" type="ORF">PGT21_022974</name>
        <name evidence="3" type="ORF">PGT21_023069</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000168">
        <property type="protein sequence ID" value="KAA1072604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M004"/>
    <dbReference type="OrthoDB" id="2500748at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1072604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="465" mass="52512" checksum="53F5E08F50929101" modified="2019-11-13" version="1">MDDIFFTDEASDDDHTERRAGEVPLETLAEGSNRRRTTAKNRTQQRQTAQNLSKIITQATDEKPHHREKVLRSLQTFTRAMMGLSPDAKPEELPTTASAEELASWDTWRERRKDAFSQRLKEATDKHPDANEQEIKHIRKRVIDQLKSRLVPVTFHASPFAANSAVSIHVKREMEADLAKHGFPRFTFNWDAAFAHDVLWNSVAVDITVRNWVPWAKRTHVDVVESAIPGIKTRFFQWITNQGVTRAKLKLTPADLAAQRKQAQYERSTKRKIATLRADTFLAVYPDKKILGHLLRDPDAVSDFEEDDANSMPTRVSAHWRSRGMEAIVRSLDQVALKRARSTQKKMSVRGLLDRKDVRAPTDEERNNQLVPARFPKDAYDSNYLLEQGEFQADYITTESMDIENLGMEMVRKSFGTGRCSLADNGSSGPTRPPPPGTHATTQQPLATATGNTAGPSGSEVQMSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M159</accession>
    <name>A0A5B0M159_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026662</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M159"/>
    <dbReference type="OrthoDB" id="2509441at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="622" mass="71522" checksum="99E42CC9D26650E7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3K6</accession>
    <name>A0A5B0M3K6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AARP2CN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_018011</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. TSR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3K6"/>
    <dbReference type="OrthoDB" id="119302at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AARP2CN" evidence="3">
        <location>
            <begin position="92"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1071737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="406" mass="45570" checksum="8AAA1BF5E8845FBD" modified="2019-11-13" version="1">MGCTPSRREGLLPTRGCTTTPVPHHPTPYTTRAMQALGGPTLGVLGLVSHLESLNGTRETQKTRESLTSFLRYFFPKSLLLNRLVSVDRPEEILVAVRSILAKLPNRASNGLPLGWREGRARLVAEKIDWEEGTLKVTGHVRGGRFSANRLVHLPFFGDFRVSMMTMAEDGKVISVRTNEEADDLVSENCPSVGDELMAEQTWPTEEEIASAPAHQQKTAGNKKARCVPVGTSEYQAAWIVDEDGEGLEDDEEEEEEEDGDIEFDKSPDQLEEDDDDEDEMEDLKVDNQAGSSKDVHFMDLSDEAEQADLAQYRADRQRERDRAAKEDEEFPDEIDTPLDIPARERFARYRGMKSLRTSEWDPYENLPIDYSKIFAFQGWKSMGRKLAHKANEQEAGAVSTMGICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3P7</accession>
    <name>A0A5B0M3P7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005591</name>
        <name evidence="3" type="ORF">PGTUg99_016059</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000211">
        <property type="protein sequence ID" value="KAA1123149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3P7"/>
    <dbReference type="OrthoDB" id="671439at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01838">
        <property type="entry name" value="Isoamyl_acetate_hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045136">
        <property type="entry name" value="Iah1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209:SF19">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="277" mass="31090" checksum="5D1A95B86F419B37" modified="2019-11-13" version="1">MVTVRPTQSEMSTTKCEPFSMDELVMFGDSITQFAWQAGGTGSALADYYQRRLDVVNRGFSGYNTTWGLHVAKKIFPAKSPESYSCPRKRIVTIWFGANDAVIPPKPQTVTPEQFVENMNKLIEMVQAHASPTNSQQDQSQLLIVLITPPPISVALRAADLASRFPDWRPENMDRDPVRTRLFAELVCQVAQQKGLPVIDTWTAITKAADESQGGLSTYLVDGLHLTPAGYEIVTQEFKSILTRHYPSFLPESLPHDFPWWNDIDAQHPENSFPECC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3Y3</accession>
    <name>A0A5B0M3Y3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022003</name>
        <name evidence="3" type="ORF">PGTUg99_014504</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000175">
        <property type="protein sequence ID" value="KAA1125753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3Y3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="73" mass="7782" checksum="919691799CD97451" modified="2019-11-13" version="1">MIKSISPLAESAPTPTTPTSTRRLRPRVPPVVYTARPRSVVHINTPDSIIGDGQITASSWKPTMSASCLRAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M573</accession>
    <name>A0A5B0M573_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M573"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="68" mass="7803" checksum="EC5ED2B9E99D659E" modified="2019-11-13" version="1">MHHHMIKLSHQLFRSFSSSEKWSSINVGLMERRNDDLAGQTNVVVAIVPIINRIITNKMVVVVEDDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M920</accession>
    <name>A0A5B0M920_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DSBA-like thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031306</name>
        <name evidence="1" type="ORF">PGTUg99_002759</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000476">
        <property type="protein sequence ID" value="KAA1072779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M920"/>
    <dbReference type="OrthoDB" id="4664297at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="8416" checksum="75F57F2213EF9E28" modified="2019-11-13" version="1">MSKRLTIRLSYDLVSPWSYFAYVVLKRYRPIWDFELILNPVWLGGTTCLQSNRMDVKLLITGLFRIFVMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9X4</accession>
    <name>A0A5B0M9X4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_017833</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9X4"/>
    <dbReference type="OrthoDB" id="10280802at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="501"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="511"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1073615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="524" mass="58986" checksum="AEE2E19A58AE67FD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MA46</accession>
    <name>A0A5B0MA46_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MA46"/>
    <dbReference type="OrthoDB" id="10433391at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="316" mass="36749" checksum="598A47466532611D" modified="2019-11-13" version="1">MDIDQSLGESFLVGFRNAGFGIPGDQLTRNPELQEHPTGSKNQLKNNPLNPSDREQENMLLQEHAGPSSSMGKAQIEPADAIKLRIKAIMDKEKFPEEKFKMELAHEKWMLHQIRAVCDKFARILHRLRVKIHHPLFSYAEIHARGLPLMITKVKDTDLRTTTFCWITNTDGNYVESRRLMKCFISLITFITYYHSNMLKKLHIEPGALQSRNQAITEWLCEEIVHPLIGFPIIGDITAEKMDLILAKKEPQFGKMNQFLSRFFSQPKIPPRTLSRVLINYWYFSGKTQMWKKYAKHQLDQEALKREIDRIISDTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAV0</accession>
    <name>A0A5B0MAV0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPL35_1</name>
        <name evidence="5" type="ORF">PGT21_010657</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAV0"/>
    <dbReference type="OMA" id="VMNQKAR"/>
    <dbReference type="OrthoDB" id="528635at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000002">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_uL29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1073389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="124" mass="14411" checksum="5B79DD7223FDD689" modified="2019-11-13" version="1">MTSKVRAHELVTKSKADLTKQLEELKVELVGLRVHKVVGGSSSKLTRINTVRKAIARVLTVIQSKTRENLKQLYKGKKHLPLDLRAKKTRAIRRRLTKHEKNLKTEKQKKRDIHFPKRKYALKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBD2</accession>
    <name>A0A5B0MBD2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006974</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBD2"/>
    <dbReference type="OrthoDB" id="10392340at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="152" mass="16881" checksum="FE44AE3C672CAB54" modified="2019-11-13" version="1">MSYWTTVKCFCEDQVSVGFGICMVRHLSTPAKYLEDSRRSRYVCQGVGVWQDFGAAPIIPPASPSSILSGSKHNDGSNLHPAPNQEMNAMRRQVQTSFDMSYWTTLSTPAKHLEDTKRSRYACQGVGVWQDFGAAPIIPPATEVITPLRTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MD47</accession>
    <name>A0A5B0MD47_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MD47"/>
    <dbReference type="OrthoDB" id="2503640at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022996919" description="Secreted protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="28404" checksum="120038B81E773828" modified="2019-11-13" version="1">MLSFNFFCHALPVISMIGPFVNAGFLPQEPVHFTRSSSVTESSTSSKTTSIKSFHSSVITQPAVVVRSFPLSQDGVNCQYQAFPQGHQGIQSNSYATGSAPSSRMVANLTPHNCHVRCTPPSPTSPNKHDCQKIINTMRNMKQRQITIEPRNWLYVSYRQCAVVFENPGAQNVAVVYQWNRLADQAERLQSQCAGGSSTSTPGYGSGSKSTGTCFFSSCDLNDSTHPFNAARKTITNAKTSKVENSGVMITFQRNKYVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDS8</accession>
    <name>A0A5B0MDS8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDS8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="271" mass="30381" checksum="2268F7E68C3D8423" modified="2019-11-13" version="1">MHISLNDFNTLSIHSRSEDMYTMGRVFRKTPQQTDGFRPERPQLGCEASNGRSGGPGIIWTLQKASKGCLAGETIQRMVYSPVSIGHLIGSVRWTTRKPSIGHFSECPIDGFHPGPAVEKKGSIVEKLFKRWSMVEKRSKNAARGLLILPSDGWFGPTIRVQQYASRIQWMLLAGPRSDGIWYIRLGVQMPSTTPGGVFTTCGVTPPERPASRLWTDHDLRQFAETVHVITTFLPLSSPRLPFKLQEVGFTGVTDQQIHRPEVLDYLQYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ME50</accession>
    <name>A0A5B0ME50_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ME50"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="94" mass="10194" checksum="1E6488E7F7CC4071" modified="2019-11-13" version="1">MHERCVAVTVLSCPGVMSFEANRQNVNCRNSVRISGTVAVTSQSRLENAVDNHPYFSGTGPIPMLRGTKRLPPSKGRFGSIIQPSRIPPVLTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEP9</accession>
    <name>A0A5B0MEP9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEP9"/>
    <dbReference type="OrthoDB" id="10510810at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="256" mass="27906" checksum="F92343FEBAAB2EA4" modified="2019-11-13" version="1">MADDHRTELKPGHKPYPKPATTLSRTGRSTPIENLLRTAIKKQQASDATDALRRKVGKTFANMTGANQGTSGTLSKGKGPARSESPEETTFQPRITDKPATASSSSNWRRTDAPPHISDANVTMQLLAKLKAQRDADQICHDREDARLQQSVMSCGGLELETLHMDPLDPQETPWLSFIVGSLGFTPDRCKRPLGNRSLALERPPDPQIQLGTALRPSFDELHPPRPLDPDGSSETVWSGPQILGFSSGTALRPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKJ2</accession>
    <name>A0A5B0MKJ2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012430</name>
        <name evidence="3" type="ORF">PGTUg99_014783</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKJ2"/>
    <dbReference type="OrthoDB" id="10529636at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137256" description="Secreted protein" evidence="1">
        <location>
            <begin position="35"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12573" checksum="9230C82DC786DF72" modified="2019-11-13" version="1">MPMGLLQEGRKTIAMQLLRLVLVSAAILVGNIQSESDGSFTCDQQIVDGKPATVAFCGHPNYNDHRHHKTPTSFSSFQPANPVGRSPVDHYTCVGIAPLLAYCCIPNTMGDVSNKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLC6</accession>
    <name>A0A5B0MLC6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_019498</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLC6"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000022">
        <property type="entry name" value="Cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="6">
        <location>
            <begin position="23"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1076799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="363" mass="38879" checksum="7E7AF17B73387BE7" modified="2019-11-13" version="1">MSQANNGVPEQFSGYAAHDEKAGKALDVKPYKYTPQKWHEGLVDIKITHCSICGSCLHTLQNGWPSPTHYPAICGHEIVGEIVQAGKESGHKVGSRVGVGAQAGSCGSCENCKSDLETYCQKGMIGTYQGKWADGSVAQGGYADYVRVQGKFAIEIPKELSSETAAPMMCAGITTYNPVKSGGAGPGKKVAIVGIGGLGHLGIQWAKALGADVYAISHSDSKKEDAEKLGVKPENFIISKDEKETSQKWANSFDLIVCTSNQHNLPVDTLFFPLLKPQGRLTIVGLPEEKIPAFYGHALVGKGISFGGSLIGPPAMIKEMLEVAVKHDVKSWTSSHPMAEISQKLKDMDAGKARYRFVMTNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMS4</accession>
    <name>A0A5B0MMS4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Endodeoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SPO11_8</name>
        <name evidence="3" type="synonym">SPO11_4</name>
        <name evidence="2" type="ORF">PGT21_028775</name>
        <name evidence="3" type="ORF">PGTUg99_012327</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000274">
        <property type="protein sequence ID" value="KAA1113893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMS4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="167" mass="18649" checksum="001DB067C8BE97E0" modified="2019-11-13" version="1">MLSSDSNEVESILSWTSEERRSEPARPADSTGSSWPDDQAESILDMDEGSSTWSGVERFSDDAQVESDFQELVDHDSVSTKSSDKQHGSLEKESGAVATLKKIEAFALDILQQINRSGNPEKHRKLRFPRDTRNRSSGSGIRELSQLLRVLELSHDAILGKMIMTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MN96</accession>
    <name>A0A5B0MN96_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MN96"/>
    <dbReference type="OrthoDB" id="3142841at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052979">
        <property type="entry name" value="Adenylate-forming_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="506" mass="57144" checksum="9A919444D75A8783" modified="2019-11-13" version="1">MEGKYAQDPVQEHNQRVPLSPFDTRQSYAAPLSPLIGGPSFPGKDYPINEKKHLSALQVYEKQPIPVPKPAASNPRPVVLNKSLKFWTWFSPYRQMLLLVVAVEAIMVIITLLGGWHFARTHLPAMVTGNLLVAIAIRSEWVMRFLYWVSIKLFRRWTPLKFRVLVVAILYHIGGLHSGCGIGAMMWLLLAWTYHIMNKDLYHSLVMASLFVSMIVVVATCFVATPLVRAIHHNIFEITHRFLGWLGIITSLIFVIVSSWWDVNTQSWRTSSRYLIRQEEFWFMIAIIPLIIGQWITVRHVPVVVTAPSSKASVIRVPGGLTSGLHTRVSRGGLKEWHIFGSISEGKHADCHYIVMAVQGGFTRAMNKDQPETLYTKTWKPCGLPYFSRLFNRGVAICTGSGIGAVGSTCIQHGDWFLIWIGADLEKTYGSEFINFIKSKIEPERLLIWDTKGPLGRPDVNVELEKVYKQWNAQVALFIGSPALNKSVLRTSRARGIPVFGSIWDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNN7</accession>
    <name>A0A5B0MNN7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">endo-1,4-beta-xylanase</fullName>
            <ecNumber evidence="3">3.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_022264</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-xylosidic linkages in xylans.</text>
            <dbReference type="EC" id="3.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNN7"/>
    <dbReference type="OrthoDB" id="3055998at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031158">
        <property type="entry name" value="GH10_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490:SF88">
        <property type="entry name" value="BETA-XYLANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00633">
        <property type="entry name" value="Glyco_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00591">
        <property type="entry name" value="GH10_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51760">
        <property type="entry name" value="GH10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023026705" description="endo-1,4-beta-xylanase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="GH10" evidence="12">
        <location>
            <begin position="41"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1077858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="319" mass="35996" checksum="CAACF70C6824535F" modified="2019-11-13" version="1">MPSMRHRVLFVLLFNFSHIIWSITAPEPKPNPTRSTKQPAGARKLAVQSVNKPLMGIGITADSLNDPRYKGVVTKYFSVITPGNELKWGTLEPNPGAYNIAGARSQYPGWIDGITKVGDMRQRMSMILEFVIKNYGRNAIAMDVCNEILDKNGNPDNLPWKRIMGNKWYEDAFRMAKQFRDQYAPQMLLFLNDFGAEYMNPKADGLLAIATSLYKQKLLDAVGFQCHFAASHIPRHVFQKNLERFTAVGLKVAITEIDMRIQMNEQPKAVQKDLAAKTADYEVIYGICRRVKGCVSVTAWGVTPSDSWIGHIEYPGMVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQD4</accession>
    <name>A0A5B0MQD4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rho GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CDC42_2</name>
        <name evidence="11" type="ORF">PGT21_028920</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQD4"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01874">
        <property type="entry name" value="Cdc42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000236">
        <property type="entry name" value="Cell division control protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037874">
        <property type="entry name" value="Cdc42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1078119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="195" mass="21590" checksum="7171A12889F33D0B" modified="2019-11-13" version="1">MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGDDPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVTSPASFENVKEKWFPEVHHHCPGVPCLIVGTQVDLRDDGAVIEKLARQKQRPVQPDAGERLARELGAVKYVECSALTQKGLKNVFDEAIVAALEPPVTKSKRRCSLNLHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTL4</accession>
    <name>A0A5B0MTL4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTL4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="143" mass="16034" checksum="258886C8DC184E0C" modified="2019-11-13" version="1">MNTHPSSAAAADFGISLEPPVSPPNRDKSRYVFGGPVTYIKRSFWNRHGLPTEHVIDISTKYVSSLFLKKTSQIMNGEIFASNIRVDDPEDPRAIREFLYIAALFHDDDDDEKEREVKVQPATLVLGVAILFPICTSTEDHTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MY09</accession>
    <name>A0A5B0MY09_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019294</name>
        <name evidence="3" type="ORF">PGTUg99_019824</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000378">
        <property type="protein sequence ID" value="KAA1092106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MY09"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="129" mass="14599" checksum="645C20E6BB86AD71" modified="2019-11-13" version="1">MVIDDDSSSNSRPESIPFGPTTEWGNTQPAAPPVNAANPNPSARDMMEFDYNTIQNRLVGANPYPFCTQVYREEDLLFLFQNVANNFIHLSNKYPASTLGEIDIAEILEEGESKFLLSLEFNDWFGERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0M4</accession>
    <name>A0A5B0N0M4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014672</name>
        <name evidence="4" type="ORF">PGTUg99_016289</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000408">
        <property type="protein sequence ID" value="KAA1087997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0M4"/>
    <dbReference type="OrthoDB" id="2505725at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="919"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="570"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="813"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="828"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="854"/>
            <end position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1082791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1087997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="976" mass="110261" checksum="6CA5B26051B14A81" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1I0</accession>
    <name>A0A5B0N1I0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1I0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="13850" checksum="F2246C368775E874" modified="2019-11-13" version="1">MRSSNDRGVLSKAIGIANRGWESMQFVSGSTGVDDHPDSPNARVEPEFLELLRQARELSKQTPDHVIENINAVPSSTNDTSAERSHIWAEAKEAQEAGRQSTSFSQNIQRSRRTNKADRNLFGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1P0</accession>
    <name>A0A5B0N1P0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1P0"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038921">
        <property type="entry name" value="YOR389W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204:SF1">
        <property type="entry name" value="ENTEROTOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204">
        <property type="entry name" value="YALI0A21131P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022931891" evidence="1">
        <location>
            <begin position="28"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="571" mass="64224" checksum="768D8EA56E6C2DE2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2U7</accession>
    <name>A0A5B0N2U7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Mitochondrial tricarboxylate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_003870</name>
        <name evidence="13" type="ORF">PGT21_005031</name>
        <name evidence="14" type="ORF">PGTUg99_008292</name>
        <name evidence="11" type="ORF">PGTUg99_036346</name>
    </gene>
    <organism evidence="11 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="11 16">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000113">
        <property type="protein sequence ID" value="KAA1130080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2U7"/>
    <dbReference type="OMA" id="AWYAGCT"/>
    <dbReference type="OrthoDB" id="44467at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000007">
        <property type="entry name" value="Mitochondrial tricarboxylate transport protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF4">
        <property type="entry name" value="TRICARBOXYLATE TRANSPORT PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="15"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="114"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="211"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1083577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1093941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1108234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1130080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="32330" checksum="9D5749C3D71A090C" modified="2019-11-13" version="1">MASSSSTSKTGNKSANPAASLIAGAFAGSIEGFVTYPTEFVKTRSQFRSATAIGKPPGPIEILKTTIARDGVKGLYSGCSALVIGNGLKAGVRFFSYEKFKKALVDQDGKLTGPRSLLAGLGAGMFEALIAVTPSETIKTKLVNESMKPNPRFTGLVQGTKEIIKIEGIGGIYRGLFPVMMRQGANSAVRFSTYSSLKAVFQGNVSPGQQLPSFMTFAIGGVAGVVTVYCTMPLDVIKTRMQSLEAKTKYSNSFDCAYQIFKFEGISRFWKGTTPRLIRLSLSGGIVFTVYENVIKLMGGLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N314</accession>
    <name>A0A5B0N314_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N314"/>
    <dbReference type="OrthoDB" id="10495682at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="29266" checksum="D00D62B6DCBC9376" modified="2019-11-13" version="1">MYSRNWTAGENKYIQAQIKQITKDNPHATGSVPFACIPRLLNDSDKAIPSSEILNTSSSPTDDAKSTATLAKVSVEEGTKAVQSPTSPNHDHLYSDSNSESDASSVVPPKKRGRYVSKPKATKQKPMKAPKKAKPSASKVKKKGPLAKPEALKAKSHAEIPWDRMLACQKADSEKNKKEEVENLKALAHRCPGFGEKYLGNVRYLSSSAPAQGGKPQKTEVRSRPSSSPILPSTLTNEASAGTGNTKGLKVSSVPPRRRSLRKSDPAAGSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N349</accession>
    <name>A0A5B0N349_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cell cycle serine/threonine-protein kinase cdc5/MSD2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CDC5_1</name>
        <name evidence="11" type="ORF">PGT21_015591</name>
        <name evidence="10" type="ORF">PGTUg99_017742</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N349"/>
    <dbReference type="OrthoDB" id="408964at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000647">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000091">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="65"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="9">
        <location>
            <begin position="767"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="480"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="548"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="940"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1019"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="484"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="548"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="600"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="635"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="691"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="947"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="968"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1020"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1083242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1094270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1039" mass="116785" checksum="3E3D7A9CB8CD70A9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4V4</accession>
    <name>A0A5B0N4V4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022626</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4V4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="122" mass="13287" checksum="E3F48192DA1AD498" modified="2019-11-13" version="1">MILPAGQSGVLLPALGVGSIPNFFSPHRRNSHLTVNLSASDDLDRTRIPKDREPSKVRLVRLPLPLGNFSTTSRAIDRALPVSSLASVIPVDKLCHIPCLLTEVVLGCKEAIELRGTDQDWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5T2</accession>
    <name>A0A5B0N5T2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5T2"/>
    <dbReference type="OrthoDB" id="2516492at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="10718" checksum="7F280D30DD32AA75" modified="2019-11-13" version="1">MASQTPKSTQNDPSGGKNTESYSTDHSQTQRHHSSRASSLVIAPKMVAPSSDSRVRLTRPAPHKRPAEAERSSNSESANLRWAGRGVIPTMLPAAVRQGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7F2</accession>
    <name>A0A5B0N7F2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016335</name>
        <name evidence="3" type="ORF">PGTUg99_016502</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7F2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="88" mass="9883" checksum="BC427A6237B840E9" modified="2019-11-13" version="1">MAPEKPPNNVPSTPPNTSEDPSVPQSTRRESSRTRTPISRPGFIRTASDSRRALIGIPNQQRRRPGQDYDSWTIFEGHLAFDRILVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NB57</accession>
    <name>A0A5B0NB57_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="4">eIF3j</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 30 kDa subunit homolog</fullName>
            <shortName evidence="4">eIF-3 30 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">HCR1_1</name>
        <name evidence="4" type="synonym">HCR1</name>
        <name evidence="6" type="ORF">PGT21_005536</name>
        <name evidence="7" type="ORF">PGTUg99_012206</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000415">
        <property type="protein sequence ID" value="KAA1085238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NB57"/>
    <dbReference type="OrthoDB" id="20381at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="28"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1077387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1085238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="254" mass="27679" checksum="6AC76D0ABF05BAAB" modified="2019-11-13" version="1">MSDWEASDHEEAANAGISVAAGVSAIKKNKWADEDADEDVKDDWEASESEDEKSKNEPAKPKGPVRNKGITKMKIAEKEAEEALRLAEQEALAQQAADPVLRKKLEQTRTIQSDMENARGLFGDAVVSNPNSSEDPMKMDPKTKADFESMSSALAQLIVSKHGNKPLYPLFVEHFFRQLSEPLSDVQTRKTASILTTIANEKQRAAKDAKKGKGKLKPTLGGGVVSGASKGGRGVNADLEVYDQALDDEFDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGG6</accession>
    <name>A0A5B0NGG6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-xylosidase C-terminal Concanavalin A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_005243</name>
        <name evidence="6" type="ORF">PGTUg99_023938</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGG6"/>
    <dbReference type="OrthoDB" id="2496341at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18822">
        <property type="entry name" value="GH43_CtGH43-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22925">
        <property type="entry name" value="GLYCOSYL HYDROLASE 43 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22925:SF3">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366264" description="Beta-xylosidase C-terminal Concanavalin A-like domain-containing protein" evidence="5">
        <location>
            <begin position="17"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1088395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1105382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="63125" checksum="329B107F43C61F6B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJJ1</accession>
    <name>A0A5B0NJJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Member of Set1p complex, histone methyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SWD2_1</name>
        <name evidence="9" type="synonym">SWD2_2</name>
        <name evidence="10" type="ORF">PGT21_003032</name>
        <name evidence="9" type="ORF">PGT21_003164</name>
        <name evidence="8" type="ORF">PGTUg99_016927</name>
    </gene>
    <organism evidence="8 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJJ1"/>
    <dbReference type="OrthoDB" id="27537at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001785">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000550">
        <property type="entry name" value="WD repeat-containing protein 82-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="120"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="276"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1088340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1105328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1114287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="385" mass="42103" checksum="2D89AA195722ACF1" modified="2019-11-13" version="1">MSSAQTTSGGGPPKVLLTPELMSSLKMAKIFRTHNAPGSSFTSISFDDRGEHLITTAEDETLQLFGARNGKHLKQAFSKKYGCSLARFTHKSSAVVHASTKEDDTIRYLSLHDNTYIRYFKGHKRRVVSLEMSPRDDTVLSGSTDDTVRLWDLRSPSCQGVLNIAGQPCVAFDPSGLVFAIALNMKQTILLYDLREFDSEPFIARHIEDPVLARISYPPPPPNLFTSLKFSNDGSKLLVGTAGDVHYVLDAFNGDILARLEGHQGLGYAPPLDSMIPGQSGSSEETTWTPDSRYVVSGSADGKVSVWDVQPPKDDPIHAMRAPQSPHATWQPLKIYDGHLGNPTRCVAFNPRLGMLATAATELSFWLPGSTDGTNNDHADVEMNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJJ8</accession>
    <name>A0A5B0NJJ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJJ8"/>
    <dbReference type="OrthoDB" id="2272314at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023115781" evidence="1">
        <location>
            <begin position="27"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="131" mass="14774" checksum="A1BD5DD603ED4E8B" modified="2019-11-13" version="1">MLVLFISRTLNLRLVVFLCPFIKIFDTPLTIVSQDRVSPVVLRSCPTGEHRLQSCNIQCVDCGALHWPEERAVIDIKAKKNKFSICCQKGNVVLPCASLSAPQIPRFLQDLFIGTTRGEYLTLHDFTDLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NK52</accession>
    <name>A0A5B0NK52_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">COP9 signalosome (CSN) subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CSN12_1</name>
        <name evidence="4" type="synonym">CSN12_2</name>
        <name evidence="4" type="ORF">PGT21_002622</name>
        <name evidence="3" type="ORF">PGTUg99_014410</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NK52"/>
    <dbReference type="OrthoDB" id="10252687at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001238">
        <property type="entry name" value="PCI domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="219"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1088318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1105306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="419" mass="47864" checksum="1A9BE102311074FF" modified="2019-11-13" version="1">MPTLKPSKYAEEHIAPAVIGKFGEALADCFSISDSHAKNLYQSSSIPLSSAENQIRRKLNSHQPWDSLTIAHLRVIHAVHSDSNSSNFLAIFDAQKSLVSAYNDWFRTDNTAWSLPVLYVIYRDLRAFATRADEALLLQGEKAAKLAEAARLIQVGFGLCCSDRTSAGDTKKLGVLYMASLLFKIYFKLKSTALCKNVIRGVENAGLLDGFQVPIAHRVTYRYYMGVLSFLQEDYEKAEDHLYFAFNNCYRNKRRNRDLIMNYLVPLRLLKGKRPIPALLRQFNQLSDLYQPFITAVRLGNIELFDRHLSQVEKQLMKRGTYLIVERCRDVCLCNFVKLVHRLKAPAHQIPLDSFRKIAYEVEDEHEEEDGNSKLEEVECLLANLIAQDRVRGYIHHQAKMLVLSKVDAFPAQTVIRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNV1</accession>
    <name>A0A5B0NNV1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_003581</name>
        <name evidence="3" type="ORF">PGTUg99_032850</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNV1"/>
    <dbReference type="OMA" id="ICCCAKL"/>
    <dbReference type="OrthoDB" id="2518053at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137511" evidence="2">
        <location>
            <begin position="26"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="243" mass="23520" checksum="FEC5A3A06A19C5E6" modified="2019-11-13" version="1">MNILSPAAFLIILELSGQLPQFAAGQGLLGSGPPGGAPGPKPDGPKLDSPPGMGGGPVKTDGPKPDSPPGKDMGAPPGKDMGGPSKGLGLPSPGPNSSASASAAAAASASVSVDGSTKTSSDSSKSGGPPGGGGTWSLTSPSGQTCSCTCKDEPKPPPPKCDSPPPITSGGGDTPPPPPPDKKDSPPPPPDKKDSPPPPPPDNKGPPDTPGITSPSAAPSVIFGAPNWISESFLLGTLMYILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQ43</accession>
    <name>A0A5B0NQ43_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021449</name>
        <name evidence="3" type="ORF">PGTUg99_009193</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000178">
        <property type="protein sequence ID" value="KAA1125422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQ43"/>
    <dbReference type="OrthoDB" id="2501890at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="459" mass="51121" checksum="E28015225C9DBFE9" modified="2019-11-13" version="1">MDPSIELPQVDSADVSLPTYKQVITDHPSATKSTKSAKKKKPRAPTKKKVKARGKSLTPEVISDGDTEDQVQKVEVTTTFKLYVLDVDRSKKAKKVWLAISPPDPLLIDITASPKEKATTYDEFNTLVTTRCEAAVKNTGQILTDGLTTGSPEIGWFVSIARVEGFKKGKPFLLSDVVAYNSWMEALVKVEADESNLSIEMVSPNKVAKLQHDAEVLAKDAIRKEAKRLITLRRQEKKAQEADGRKRKQSDETDDSEEEVSDEEEEFDKDSIKLFMRQLYATHMANAMYDRHIPVYINPTEQSKFFRLSNATCQKWARALAKAQPGVSIQSPPKEFKFEVLPLKQLVPPVKNVTAANECTHHNCGRDASPLLQVGSDGPQPGEEVPILDYLNFIGLKNAAEVGDVLEKNDLQNYKIFRSSNLDRPALRGLGLTLGVVTQLCDCVSKFERHLANQEANRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWI6</accession>
    <name>A0A5B0NWI6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025667</name>
        <name evidence="3" type="ORF">PGTUg99_005622</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000042">
        <property type="protein sequence ID" value="KAA1135001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWI6"/>
    <dbReference type="OrthoDB" id="10363421at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="130" mass="14634" checksum="224640E9D2B51C9F" modified="2019-11-13" version="1">MHAEEKQGGHTDVISVDNGGEIPGRGAERLFNRSSSNPQKQYTVTLDFGGNYRGCRQRLNNIKDVPNALPVHTGEELSLRPVSNIGALEGFAPPLENRFLQTMAITLWSMQYRRRQKAKMTTTGEARMWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NY79</accession>
    <name>A0A5B0NY79_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008312</name>
        <name evidence="3" type="ORF">PGTUg99_021663</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NY79"/>
    <dbReference type="OrthoDB" id="2499685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="571" mass="62994" checksum="3E945BE957745615" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYH9</accession>
    <name>A0A5B0NYH9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Malate synthase</fullName>
            <ecNumber evidence="2 8">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_005457</name>
        <name evidence="13" type="ORF">PGTUg99_007497</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the malate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="2 8"/>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NYH9"/>
    <dbReference type="OrthoDB" id="186072at2759"/>
    <dbReference type="UniPathway" id="UPA00703">
        <property type="reaction ID" value="UER00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00727">
        <property type="entry name" value="malate_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1220.12:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.360:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="entry name" value="Malate synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="entry name" value="Malate synthase, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019830">
        <property type="entry name" value="Malate_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048355">
        <property type="entry name" value="MS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048356">
        <property type="entry name" value="MS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902:SF1">
        <property type="entry name" value="MALATE SYNTHASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20659">
        <property type="entry name" value="MS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20656">
        <property type="entry name" value="MS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="MS_TIM-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001363">
        <property type="entry name" value="Malate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="Malate synthase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00510">
        <property type="entry name" value="MALATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Malate synthase N-terminal" evidence="10">
        <location>
            <begin position="22"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase TIM barrel" evidence="9">
        <location>
            <begin position="162"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase C-terminal" evidence="11">
        <location>
            <begin position="412"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001363-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1092504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1093604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="542" mass="60829" checksum="A401B2E805E48AB7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZC8</accession>
    <name>A0A5B0NZC8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger, C3HC4 type (RING finger)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RHA2A</name>
        <name evidence="4" type="ORF">PGT21_025264</name>
        <name evidence="5" type="ORF">PGTUg99_023300</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZC8"/>
    <dbReference type="OrthoDB" id="1681166at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF434">
        <property type="entry name" value="GB|AAD18119.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474091" evidence="2">
        <location>
            <begin position="20"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="60"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1094571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1129013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="177" mass="19697" checksum="F3F73FD932C11163" modified="2019-11-13" version="1">MVSIGILMVSMFLHQVIISKPTIPGPDHQLASVVSNALLISSHHNIKRSLNDGGSSSNMCSICKNAVEEGQVRRWPNCGHPFHSGCMSPWFVQTGSTCPLCRGSGVNREQTTVLWQQEPLPRGQENSPHNPNPWTRPSQYNSEFANCPTCSTNCRATIPPIEYYCNTCKAFWRKPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0J6</accession>
    <name>A0A5B0P0J6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_050213</name>
        <name evidence="4" type="ORF">PGT21_050291</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0J6"/>
    <dbReference type="OrthoDB" id="2505969at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="3">
        <location>
            <begin position="146"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="948"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="956"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1080869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1094432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="988" mass="112951" checksum="C035CB524E41738F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1L0</accession>
    <name>A0A5B0P1L0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014835</name>
        <name evidence="1" type="ORF">PGTUg99_012929</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1L0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1095281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="391" mass="46446" checksum="2F039F349E645E61" modified="2019-11-13" version="1">MPKLNEGSHSEDLLSDSDISWEDISPTIEDIMYKRTLVIHYVEEFLQDVRQRKLIPQFNPIPRVRKSQLIEIAYLVFEHLFPIYTQNEAVTQYNLNVELEVHKFPLFIQDFFLKFMCKDCQSQRIRQEQLLNTISEAHQALENEFRGKYIGLRVLVAKMEVEFKSGSLSMQEIKQKHNSEIWDSMVKCELQLMHPHESIIIREHITKLGWVKGKIVGNLSKISFRDAERLINLLKCGTQLFKTVPLFNRLDYCMKIEDEEKKNLIFMDAGRKLESLYGKIPEEECQELHKVHNFSRRRLSRSDYSFWKEYIFTKLTKLPPLSVQVLDDIIFDASRCQLLEDSRRKSLQETYGKVNEDFLSPELKERLDTLSEMWRIHEVEIRILTQEPLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9W4</accession>
    <name>A0A5B0P9W4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_050178</name>
        <name evidence="8" type="ORF">PGTUg99_036766</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9W4"/>
    <dbReference type="OrthoDB" id="2505882at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="6">
        <location>
            <begin position="153"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1097863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1117178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="275" mass="30454" checksum="7FC14EE53176B6B3" modified="2019-11-13" version="1">MNIFYEITLQISTPGLARLSNIVIFINQITEHLSTAISGTKYPPALRNACQVGLKLTNKYYSLMDMSPLYRIAIVLHPSFCNEYFKLANWEPKWISEAIRLTREMWVSVYKPKPITPPTSSAVVANKPKTGMLASLGSAAAAQGGHSSTDPLDMWLAGGLILNNNEPVNPLKWWLQQKKSGNTHGGLLQMVLDVLSCPVTSVDVERAFSFGCNYVSSKRHRLSAQSLSRGMAFAFYSKNGMIKEGVLVKWKDCIQTAKKGKSDGKGKRKVIVLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA19</accession>
    <name>A0A5B0PA19_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA19"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="235" mass="26409" checksum="23A35C78A7A42A8F" modified="2019-11-13" version="1">MPVGLPNLASLILLYELQPDLGIYSKPPMTWAGQGQNKSRRYSRTNYQLHQQLGTTNNNNTNTNNIDPSHHHLTTSHHPPSNLSLNQPAHQITNNSRKTTPHSLTSSEDYSTSNSRNSSPTNKSQPNHPQKNYSIIHQQNSSHSPYSSSPLNSSSSPPFTPSHSLLKLDVSQIQIGETLREARKHDQLCHPFPNLISQILIIINSNLIDLSNLEYKRRTNLDLINRQSSQAFQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PDN3</accession>
    <name>A0A5B0PDN3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">threonine synthase</fullName>
            <ecNumber evidence="4">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">THR4_2</name>
        <name evidence="13" type="ORF">PGT21_007061</name>
        <name evidence="14" type="ORF">PGTUg99_011391</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the threonine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000210">
        <property type="protein sequence ID" value="KAA1123224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PDN3"/>
    <dbReference type="OrthoDB" id="5203861at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01560">
        <property type="entry name" value="Thr-synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000024">
        <property type="entry name" value="Probable threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1380.10:FF:000003">
        <property type="entry name" value="THR4p Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="entry name" value="Threonine synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051166">
        <property type="entry name" value="Threonine_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690">
        <property type="entry name" value="THREONINE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690:SF1">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24857">
        <property type="entry name" value="THR4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Threonine synthase N-terminal" evidence="12">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="11">
        <location>
            <begin position="103"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="316"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="319"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1099421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1123224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="574" mass="63820" checksum="4F69FC5416933BE2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEI8</accession>
    <name>A0A5B0PEI8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEI8"/>
    <dbReference type="OrthoDB" id="248495at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="8288" checksum="EC2E384C0C658BB6" modified="2019-11-13" version="1">MPTSQHQSNSKTGDSAQEKGKSKQQLSVADEEIEQERQTHLRTIELLDRVANGEKAAGRLLPADLLEDPLDAHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PF66</accession>
    <name>A0A5B0PF66_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_69</name>
        <name evidence="2" type="ORF">PGT21_001334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2510395at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1177"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="545"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1177"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1248"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1269"/>
            <end position="1293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1312" mass="144552" checksum="BBA813EB97BDB0AB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJY6</accession>
    <name>A0A5B0PJY6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJY6"/>
    <dbReference type="OrthoDB" id="10302856at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="178" mass="19475" checksum="0DA6DD0AF3EF8884" modified="2019-11-13" version="1">MSFLPAQLNLPASPPVALDPPTLTCLSRQSIYFFQVCNRQAHSVVPIVDTLAGESFLALMKTSLKLIAMEDAPADSEQSTSQQLSPAMKRAVEAIVEQATSALRTEHQEALLDARIDFEEVEEQLTKRIDELSAQVARLENQQRGSTVSSASTIAPRRPSPSESGTSTSTRRVRRRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMC2</accession>
    <name>A0A5B0PMC2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030744</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PMC2"/>
    <dbReference type="OrthoDB" id="2506484at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="388" mass="42132" checksum="8C59B606C1119BA1" modified="2019-11-13" version="1">MNRYSTPNTSSSDFTVGEKLWILAHIFKEHQDLLSVAGRCIARYSEANIAVLCNASAYHNEIPHRHRRIPFPIVRGYLKIIDQGVSCLALDPDVSDLEQLIAPGSTYGQMYYSTSVPFLDSCTKEEKNLLIKLCGYGSAATGLIDSHLYSISGKLIAPNSKSTPVLHFDTDTVIELGLTANIPIAMADKTSVVGLGIVMSKREVADPEASTSSKNLVIVLQHTDYDPVAKAQVQFNTQYIIGGRKNLANTFGLFQLGREILISGHITGYEHAQFMWIVTIITVGHQSVNSQALITSQVGEAKPRRPGLISVGEDLSQPQAEVIVDTQILGSSSGTVEGLDVGPVHNEGQNSSNSVNYYDNVALTSGKKRTKKEILADAKRAKLNMSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNU7</accession>
    <name>A0A5B0PNU7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit OST5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_017669</name>
        <name evidence="7" type="ORF">PGTUg99_003309</name>
        <name evidence="8" type="ORF">PGTUg99_027934</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the OST5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000385">
        <property type="protein sequence ID" value="KAA1090402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000338">
        <property type="protein sequence ID" value="KAA1102334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNU7"/>
    <dbReference type="OMA" id="NITRPHE"/>
    <dbReference type="OrthoDB" id="2503643at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007915">
        <property type="entry name" value="TMEM258/Ost5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05251">
        <property type="entry name" value="Ost5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1090402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1102334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1105757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="82" mass="8652" checksum="6392CD5ED89BEDE6" modified="2019-11-13" version="1">MDDYTVATLAWKAAVPFTPDLPTHLHSSIGFALLASAFGLGFLFTTLPKTGFPTTELIPALMASLLTGFGVVFLFNAAGVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSM5</accession>
    <name>A0A5B0PSM5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_031260</name>
        <name evidence="11" type="ORF">PGTUg99_003276</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="11 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the borealin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1098939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSM5"/>
    <dbReference type="OrthoDB" id="2505648at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018867">
        <property type="entry name" value="Cell_div_borealin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16040">
        <property type="entry name" value="AUSTRALIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16040:SF7">
        <property type="entry name" value="AUSTRALIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="266"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="116"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1098939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1104757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="498" mass="55755" checksum="C64727707FBD4C95" modified="2019-11-13" version="1">MYASDTNTLSILLHREQPNQQPTTLEHEYCNTHTSTNYYPMANHKPKTKTKTKTKQPNSPNKQPPPGPSSPSAAPSQGLSGRLFGLLKKFPMQITSPSKEIPPPSSPQDDRHTTESEDGSESEQDQEEEDEEEGEGEEEEEEEEGLEEEDEEEEGDGEETEQEIVTESEEEEEEEEDPLGDEQCKAIMENYLLETSTRTNLLKNRIQTHFRPALRRRLDFELSRITPSIANVTALDYHQRDQGVLRRTMEHVARLAIEQKTISIGIKRSQSQQDQIWDEKEKDHHRKRQRQLTNNSGSVSSKSSKQTVDPHEPSRANGGNNGRAPVRSKPPTSRSNTGGAHGRKQSNASLEPTELNPRLPSTPLISEHKPVRLARRNESLLSVNGSPVISYAPDEIPGLPNSTSAATIISSKFSELFPASSTSSSSLALDHHHLPSSSSHLFGNPSNLNQLPQISQFTFVEKSKWNQLEAQLNSLEIDQIQKKKLDDLLKGCLNFGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVP2</accession>
    <name>A0A5B0PVP2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005348</name>
        <name evidence="2" type="ORF">PGTUg99_001376</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000315">
        <property type="protein sequence ID" value="KAA1104874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVP2"/>
    <dbReference type="OrthoDB" id="2498546at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="206" mass="22984" checksum="748E17A35CC04EA2" modified="2019-11-13" version="1">MIIDLLQSLPYLTPTMSLIANAALASPDAQVSLNQLIAQVKEEIFRQLKPEDHKYLIQSSRLLSLRDSNPSKCTGESNLLFNDLSVPRCWRARAGVYRAESLMILGFVDFAKEQLISTGRILETDDHLQPIDMIDRNRHEILEGLVAWNIALGKINWGIIDPERVFNFSIASKKAQTRGMITASGKLFSAGYPSSSSLSALDLKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYU2</accession>
    <name>A0A5B0PYU2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028808</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYU2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="2">
        <location>
            <begin position="208"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="504"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1106095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="538" mass="60360" checksum="9F0DC7EB3ADB1B01" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZN1</accession>
    <name>A0A5B0PZN1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034093</name>
        <name evidence="3" type="ORF">PGTUg99_033193</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZN1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="79" mass="8532" checksum="B79A65A13C41FC79" modified="2019-11-13" version="1">MKAPNFLKCLALSEDKTGGPGTESDRVISDSRRPSGMFERPLILVNTAPAKCFPDGLRDGDPLDLGRDLQQPGSEMRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZQ5</accession>
    <name>A0A5B0PZQ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033053</name>
        <name evidence="3" type="ORF">PGT21_034624</name>
        <name evidence="2" type="ORF">PGTUg99_030810</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZQ5"/>
    <dbReference type="OMA" id="ECSCNKE"/>
    <dbReference type="OrthoDB" id="2495256at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1106317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="83" mass="8090" checksum="CE0170A13771832A" modified="2019-11-13" version="1">MPACGCPDVPLDNGEVRACSCQPCSDGNLQACSCNKETGCGCTSSALPKEAANRCVCAAAGKACSCADCGCKDAPATSGSCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0D5</accession>
    <name>A0A5B0Q0D5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036788</name>
        <name evidence="3" type="ORF">PGTUg99_002228</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0D5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="141" mass="15603" checksum="FCA1E54BBA620BE4" modified="2019-11-13" version="1">MASPLPFTDAGDLRFCLGSFNLPQLSKAVLTEELPTRLIPKGFGRRNHPSLMSLNPRAQAVSLSFNSNGDTNILNASLGTHWHRPLCPLASSNKQGLWSITAQHQLTFKTVKRSCIPVKQPQSNFMHSPQGIRTTNKLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1H4</accession>
    <name>A0A5B0Q1H4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001237</name>
        <name evidence="3" type="ORF">PGTUg99_012051</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000147">
        <property type="protein sequence ID" value="KAA1127922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1H4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1127922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17945" checksum="B4D06A9DE9D2516F" modified="2019-11-13" version="1">MFPMNSFQKVYIGLAFLPIIIIFHLGQLTLVDGLYRYPEVSCMPHFDSVYGDPIASCWFDSQTKFKCRKTSCKSFDHPDRPFGKLAFSGCVHNEIPKNGVVHPRNYHHYPSGDDKWLPGKFGYVAAFDSSTQLWYNCPYTASKDNSDNYLCTDCWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q599</accession>
    <name>A0A5B0Q599_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SDA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SDA1_2</name>
        <name evidence="11" type="ORF">PGT21_005495</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for 60S pre-ribosomal subunits export to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the SDA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q599"/>
    <dbReference type="OrthoDB" id="2196187at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027312">
        <property type="entry name" value="Sda1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048292">
        <property type="entry name" value="SDA1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007949">
        <property type="entry name" value="SDA1_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012977">
        <property type="entry name" value="SDA1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730">
        <property type="entry name" value="HSDA/SDA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730:SF0">
        <property type="entry name" value="PROTEIN SDA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21638">
        <property type="entry name" value="SDA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05285">
        <property type="entry name" value="SDA1_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08158">
        <property type="entry name" value="SDA1_HEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SDA1 N-terminal" evidence="9">
        <location>
            <begin position="93"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 N-terminal" evidence="9">
        <location>
            <begin position="245"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 middle" evidence="8">
        <location>
            <begin position="625"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 C-terminal" evidence="10">
        <location>
            <begin position="829"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="536"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="587"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="780"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="536"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="595"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="622"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="637"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="655"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="675"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="780"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="865"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1108237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="882" mass="96540" checksum="326FA478E093ACBB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5W7</accession>
    <name>A0A5B0Q5W7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009483</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5W7"/>
    <dbReference type="OrthoDB" id="2499224at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="358"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="661" mass="74007" checksum="F1FC5A3B2F95BC8E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA02</accession>
    <name>A0A5B0QA02_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QA02"/>
    <dbReference type="OrthoDB" id="10662372at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="430" mass="48354" checksum="3FA38352E4140CF6" modified="2019-11-13" version="1">MEGTSQLNNNNETSNAENASSNPRAEPETDLLDYSEAMTRDTSSVQQQPSGFESLESSLPSITDTALRRQTAEVSQLERGVSPALINSRATSLALETMSSRSTPVPRPVDRLPFPANTSTEMGRQVLTPIPRRNLQTQFPALEQPLAPQEEDTIMDNTELETVINLFNEQWNMFVQARENQNPRLMRVALIQAISSQEEIRVLAGGTEMLRICGNWIAREELADLERSQLAHQNQQATRLAITQTAHEHTVSPSPAPQHRLTRQSSDVTFLGAGPMQVDNNPMSPPQPQSTRPQSALVRQNPEPQAQQAQPYHQQPRLQYREGGYYQQPPQRETTVQHHAPQYQQQARIVQNYAPQPAPPPPQRQSWRGSGRNWRLPQDQTSRLLEVGDYLMRAERIAGRVFRMRGRGRARGRGRGPPPQQGEPPAQIHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAA7</accession>
    <name>A0A5B0QAA7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_002471</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAA7"/>
    <dbReference type="OMA" id="NHNVHRS"/>
    <dbReference type="OrthoDB" id="2501864at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000037">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023042701" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="250"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1109904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="433" mass="46042" checksum="9125F714E7046F6E" modified="2019-11-13" version="1">MITYILLLTSILLAPGQVANWSIPGAPQHKPLHAKRHSSSMYNWEQSWNSQTNNQITTITTSTSGTIPPDSGLHQGAASTWSYQYCYSSSSDDNQDNSAASFGDSSSTFPASQSGAGSDSGFSDEFASVPGSSNTTPVVNSPPKPVRQEQTYSPPKPSVEVPSIPKDLSPTPQPKYANVTKSPGLPRSSALSAGDTSRYSSTKSDSPSLLSTPNPESTDNKEDKKESSRLPALSDSDTGGTKKLTPSPSGSHQDWLDSHNNYRSQYGVKSLTWSEDLVEAARSEVEKCVWKHTKHNQYGENIAAGQNSPSEVVVAWVEGPNERDIFSPDSATPTHFTQVVWKETEQIGCAVKSCETIEGSNLPQAPVKLWACEYHPKGNVGGQYAKNVLAAAGGKPLAAAGSSAFASVDPDDPSRAKAGGSGLSRTTFFPAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAH6</accession>
    <name>A0A5B0QAH6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">YmL10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MRPL10_2</name>
        <name evidence="6" type="ORF">PGT21_010597</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAH6"/>
    <dbReference type="OrthoDB" id="361383at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000011">
        <property type="entry name" value="50S ribosomal subunit protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_uL15_bac-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934:SF11">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="123"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="27"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1110102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="300" mass="33714" checksum="BE3DF38E9513A4C1" modified="2019-11-13" version="1">MMTSILRTLRRPERLCQSIAIHQIAPQLASRTKKERSLPDGQGRRWASGMSNLKPAKGSTHSRKRVGRGQGSGYGGTAGRGHKGQKANKGNSPRLGFQGGQTPLTRLFPKRGAHNPTQVEMSPLNLFQLQLWIDQKRIDPTQPITMKEMLDSRIVHGIKDGVKLLGKGATDFRTENLTIIVSRASQSAIEAIERLGGRVICKFYNRLSLRALLKPHRFAAKHRFLPGDAHPVRKQDWMRYSDWETRRGYLGNLELTNQILDQVARRRAKQGWLSREQLASHVRARVQSDQAPPPPPPPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBN3</accession>
    <name>A0A5B0QBN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBN3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="85" mass="9263" checksum="16A6C11D6D715329" modified="2019-11-13" version="1">MAFGAVTFNVLYIVVVGASTSTLSRGNIYRDMTGAAIVGMHFECVAQCDQQVDKSQMYHGGDETHGDWGRVMQAIDAYVLEQVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBR4</accession>
    <name>A0A5B0QBR4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Negative regulator of mitotic exit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KEL2_2</name>
        <name evidence="8" type="ORF">PGT21_025312</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061245">
        <property type="term" value="P:establishment or maintenance of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000049">
        <property type="entry name" value="Cell polarity protein (Tea1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF456">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="153"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="512"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1505"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1642"/>
            <end position="1682"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="792"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="878"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1018"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1256"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1451"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="64"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="512"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="595"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="630"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="652"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="690"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="719"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1661"/>
            <end position="1682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1110555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1682" mass="186322" checksum="2666545B3B2594A4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC13</accession>
    <name>A0A5B0QC13_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QC13"/>
    <dbReference type="OrthoDB" id="2505225at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="596" mass="69168" checksum="FAC7EF44ECDC006F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCN8</accession>
    <name>A0A5B0QCN8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_035302</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCN8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023088622" description="CCHC-type domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1110988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="8951" checksum="6495387E619441DF" modified="2019-11-13" version="1">MKINIFISMPISVLFSHLLAMDLPANGHIQILDGVTSVQVVDATGKSPTPPPPCAHCKKYGHTSDECWPKSRQPANGQLDALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFU5</accession>
    <name>A0A5B0QFU5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFU5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="276" mass="30515" checksum="DE8262E938BFF418" modified="2019-11-13" version="1">MIQSKRAQPISYSDKNNDIETYRMHEAGRRRMKNVIETGPSQEAVRLARLESNHVGSINIARLLSVASCYEEIGNSKGYHNVPESKSGLDGQAQKRPRRISCGSSAIVRRKSEEKEGTSTSVLAKRRRLDRGGCPLKPWVKLNHPWLSILGPSELIGQQAVPLCKGTNVEPVALDASLMQDNRIPSALDFNAVFKSNLLHGLAQLALTCVVIPNTDFKPLTPMIPQVLYRTSNFTIGFSLTDRLLDQVLKRIRLRDVCILGSSIGLATTLSRTLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QI13</accession>
    <name>A0A5B0QI13_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_003108</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QI13"/>
    <dbReference type="OrthoDB" id="48651at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001085">
        <property type="entry name" value="Related to TIF3-translation initiation factor eIF-4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF11">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="94"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="153"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="418"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="488"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="599"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="439"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="509"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="574"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="599"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="638"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1112594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="648" mass="69299" checksum="3720AC4A91BF76D4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ67</accession>
    <name>A0A5B0QJ67_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025341</name>
        <name evidence="3" type="ORF">PGTUg99_024991</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ67"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="222" mass="24966" checksum="382C7C22025CB224" modified="2019-11-13" version="1">MLKGITAGCPPEHKKPRPPLSLATHSPSPISHSAAYIHSFHNSPALRNLKMRSSTVLTYLVTLSVAIGLCVCVDPDPNVGFVHSWRSPRDREATHMFSDNEVTTEVEVNRNDDGTWSICANNRLAFPVNFQLNVDGKRIVGPRVGDCRTPNMILQHTPYPVVITKTDKKPKHVSFTFYLRQSSAITVKQMDRMPSFSNRQYTLRSPRDSDDEFGPPFVKVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QM85</accession>
    <name>A0A5B0QM85_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450-dit2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_004724</name>
        <name evidence="10" type="ORF">PGTUg99_031358</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000204">
        <property type="protein sequence ID" value="KAA1124410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QM85"/>
    <dbReference type="OrthoDB" id="2498889at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1114331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1124410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="620" mass="69532" checksum="7CF5E0B528628744" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNR8</accession>
    <name>A0A5B0QNR8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NOT2/NOT3/NOT5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_027779</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNR8"/>
    <dbReference type="OrthoDB" id="25391at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1020:FF:000009">
        <property type="entry name" value="Related to CDC36-transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="432"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="158"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="267"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="319"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="579"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="319"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="590"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1114947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="650" mass="70355" checksum="D42F41C36B3890FE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP07</accession>
    <name>A0A5B0QP07_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP07"/>
    <dbReference type="OrthoDB" id="5555409at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="12460" checksum="B5F9FA30C30E843B" modified="2019-11-13" version="1">MIPIPKNSDSLYSSIKPDKIIAGLIEKFKFESDQTVLLSQFIKIVGSSDSSSLPSSSSSSLDLLPPLVKFKPIKRTGCYAVALSIDDPHSTQRWSNDSKQPPSTYLFSILVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPJ7</accession>
    <name>A0A5B0QPJ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPJ7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="153" mass="16973" checksum="4F8474BADCC5FB69" modified="2019-11-13" version="1">MQSERPRDALASQHHLDASKSIQVTLAKYEASNGRYDLFSIGHSPTKSVRWMTVFSVSIGHFELQFLIGASGRWLKKASKARSMVEKHSENAVDGRKSLQKCGRSSKNAPKVQGSDASPDVSHQIQMSREQIEVEHATTLAPSKRQQALTKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QT82</accession>
    <name>A0A5B0QT82_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QT82"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="51" mass="5681" checksum="28333139965D5F18" modified="2019-11-13" version="1">MSHPLDRPYDMSAAADLCHYSIPDRRAHLGSEQLMEEVQRGVGKRCGDLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWV9</accession>
    <name>A0A5B0QWV9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWV9"/>
    <dbReference type="OrthoDB" id="2507052at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="569" mass="64920" checksum="AA63B5D63842DF40" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXJ8</accession>
    <name>A0A5B0QXJ8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXJ8"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050152">
        <property type="term" value="F:omega-amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006528">
        <property type="term" value="P:asparagine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF30">
        <property type="entry name" value="OMEGA-AMIDASE NIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="220" mass="24069" checksum="DC6C476D6FE4FA71" modified="2019-11-13" version="1">MSTFNSNLNVQGTSFNIALIQLGGIGADKPANLTSARAKIADAVRGNDPARKPQVVVLPEIFNSPYGSGYFDKYAEVIGWHESKGADWDVNKCDSPSIKMLSSAAKEEQVWLFGGSIPERSPHDPKILYNSAPVFQPDGKLVALHRKLHLFDIDIPNKITFKESETLSAGQDPVTIVETPFGKIGLGVCYDIRFPEMAMIAARRGLSKVNKCDFPDDLDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1F8</accession>
    <name>A0A5B0R1F8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1F8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="107" mass="12062" checksum="146980213399012B" modified="2019-11-13" version="1">MHYPQLPQLMHYSLPSVRLRALEPSQQTVKDIIFLAFPPKIQHLKTVADKSIKTNNLSATIAVPLSTLPELYHRHRSLLPIQTVLLLALPIFANNEPIYCLVASIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R224</accession>
    <name>A0A5B0R224_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R224"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="87" mass="9985" checksum="0F6A02781AC7149B" modified="2019-11-13" version="1">MRFDVGLLDEVYTHPHSEESPEITARGLDTHFTSHFQLALRDLQHLRLIVFFVAHHQLLSSCLRGFFYVGLFLPELPSAFESRLCGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3L8</accession>
    <name>A0A5B0R3L8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3L8"/>
    <dbReference type="OrthoDB" id="2498672at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="4"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="408" mass="46578" checksum="1F963285EAB131E4" modified="2019-11-13" version="1">MANILDLPREIFERIFMHLITTDWEWGHELNRADCIAYVACHLRQVCRRWADWLYESHLYHTLTFNSASTAIAFANHIRERSKLLPRAKCQYLEIDHFWPWKHLPGHAYNHNEITSEALEVLIDLFSDTIVTLGLKFIEFFTLPSETIEAIGRIKNLRNLTFELSRYPSGMDEFPPTDPDGFNTLLMATRGLKSLRLGLPVSLRPESGLPSITYLHLDFQFEDLGFIISLSIALKPNLKVLSLCNCGRDFDAQTLLPVYENLRETLEGLSVSRSCFLEPILDFNFPKLRVVSVHDWSGSIANWLSQDLFSYAPIQALAIGSRSVKGLHQAFPANPLANLPKLQQLVIMNTRSSFSPTPSYLSVSKSHHVKCTYLDHGEMSLIMKLLMPTNLLQVKKPRHSLLSFIPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R6G5</accession>
    <name>A0A5B0R6G5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025444</name>
        <name evidence="2" type="ORF">PGTUg99_021830</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1120949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R6G5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="378" mass="44156" checksum="FBA4C454B99C5621" modified="2019-11-13" version="1">MQDRLVLRGFKSLARDHGPSSFHYDNSIQELYTLENIKWRRDHWDRLRSNLLPLLEQQLITLSQLLEPNHLQKEPEFTFNLIVGVQSQLVQTFDQIRSAIRILCPEPPEQLESNRINDQDFKELKRFRVRGLHRMITEEVLPEFNKLFRCSYHLITGTPVRSSLENKRIIIFTSSSRNAIKRTIVWLEGSECEFLVRNYWQKDPLNCNGRLEQLSILITRGTFNPQDPNGQVLNKRAMGLAKLIIPIIKLSRLFFKNLARWASKQTQLPSFTGMRSDQLETLSKTHEAVADYLNKFTSFLRKVVTDNGRACSGDFANNLTTLEARYEAVLFLVSFYLIPLFPDTDRPRAYDDFQDLFRTWSHQFSLAVVNLLDASKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RBV8</accession>
    <name>A0A5B0RBV8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_005039</name>
        <name evidence="6" type="ORF">PGTUg99_002491</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000209">
        <property type="protein sequence ID" value="KAA1123311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RBV8"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF416">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE GODZILLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474777" description="RING-type domain-containing protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="232"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1083717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1123311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="289" mass="32774" checksum="AB9635AD02EC91D5" modified="2019-11-13" version="1">MKLFVLSIFCSVLLAMDLQNLSGDLRYHLGDGAHFSEEIRATSEVEQRITPSNRDRTANRLRFPGSSLFDRIRHRNSGRDQGRATLQAAPEPPEITPEQEYMFQRYQQQGVNLLYIAPEQALSEVEHLRLVQAGPPTGPRSYKETDKIENTRLLGYMLKPSPPIRQDTVPLEQALKTLTSSSNAALQRSHRIGLRTSGNGPHDYHGGSSSTSDQALDEITEVLLADPDHRKCVVCLEKFKSPQRDGKNRWSFDKVLMIDGCKHYFHRSCLQAWVLGRGHRDCPTCRHEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RQF0</accession>
    <name>A0A5B0RQF0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_016940</name>
        <name evidence="13" type="ORF">PGTUg99_030452</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RQF0"/>
    <dbReference type="OrthoDB" id="406631at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046355">
        <property type="term" value="P:mannan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000251">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF39">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474775" description="mannan endo-1,4-beta-mannosidase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="11">
        <location>
            <begin position="147"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="32"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1076728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1126894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="634" mass="71462" checksum="05AB0DA0361FFA14" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S756</accession>
    <name>A0A5B0S756_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Nucleolar complex-associated protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_002301</name>
        <name evidence="10" type="ORF">PGTUg99_031730</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S756"/>
    <dbReference type="OrthoDB" id="10263597at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011501">
        <property type="entry name" value="Noc3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016903">
        <property type="entry name" value="Nucleolar_cplx-assoc_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428:SF5">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07540">
        <property type="entry name" value="NOC3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar complex-associated protein 3 N-terminal" evidence="8">
        <location>
            <begin position="364"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="CCAAT-binding factor" evidence="7">
        <location>
            <begin position="751"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="590"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="799"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="631"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="808"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1082407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1133700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="944" mass="106706" checksum="1903F1D59533E8B2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SAR5</accession>
    <name>A0A5B0SAR5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027818</name>
        <name evidence="4" type="ORF">PGTUg99_015998</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SAR5"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="2">
        <location>
            <begin position="252"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="472" mass="51661" checksum="B1247DA6B7539153" modified="2019-11-13" version="1">MYISSSTQTFKAYHLLVVPIIIISVFYSACARPSPAQGHVYPIYTQTSLAEQSTASQNTSLSSSSNILEKRMVRSCDPLPLTPETWNELELDFYLKEYPDGDRMTLIDFATSKNASNFVCGLGSICNAGQMCYPVNDNDWYILFAIQQWNSINNMLYMAIGYAISIVQATVNSLISTMFPAVDISGATHAKQSMAISASMMEVSGTLAMDLMMIMGAATGPVGWTLNVLNFAAAAGFAIYAFKQKVPKGPIMEGFGVWTDVVFHLANVERDAHDALSNAVKKVINSPISSKDGIYGVLKGGTFMNTAKLKPIPEVADQLRKVTMAMSMNLVLRDMNAYIAVGADDCYDKGPNGAWPQQDLLSYCPKQGGPMYNIIRAVGEKSENNIPNANVINEIFGFPTQLIVDVSVDCQLRNGGFNHNPYVNGKFPKSEHDECVFNLPVCDARWGGVRDSIKKHHQHVTVACRKHGGLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SE03</accession>
    <name>A0A5B0SE03_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GH15-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032330</name>
        <name evidence="2" type="ORF">PGTUg99_004515</name>
        <name evidence="4" type="ORF">PGTUg99_022353</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000424">
        <property type="protein sequence ID" value="KAA1084931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000039">
        <property type="protein sequence ID" value="KAA1135403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SE03"/>
    <dbReference type="OrthoDB" id="6123450at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000084">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF9">
        <property type="entry name" value="GLUCOAMYLASE, INTRACELLULAR SPORULATION-SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="1">
        <location>
            <begin position="66"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="578" mass="64780" checksum="0FD3FB441D76FA55" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKS3</accession>
    <name>A0A5B0LKS3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKS3"/>
    <dbReference type="OrthoDB" id="10281162at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022916185" evidence="1">
        <location>
            <begin position="28"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="368" mass="41266" checksum="49F5AAF077129E6C" modified="2019-11-13" version="1">MGLPLSPPRWVIAQVIIYLALCSSLQCGIPKTLSTIPEDRAITVFREAPYVTPEAFYRPGYVTPEINPGSRVAIAKNKIVDAFFKEAKKGRAPSIWEIKHVKEKLVPDAIQEIFSRAQQSPHSDETINALIEGLEKSLMGKAYTPNILKAAIAFQQGLLSLSTRTWKDSSKMADTIESMLQRNGDYIRQLRRNDRVGEVLQSTLGTSEMLDHKLSPNYATVDGLGKNIEFDRGLSEILQQIRKLAEADSPRSASLYKQIPKQRTGVPISRVVSDFKSPSARPQTLIKQLREILSPRTPVSFQTKTAALLLLHHTAHWHLDQTPRILTDHSEMLELRFLAANALKHLSDSEDHTLIFPDHRALINASLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLA7</accession>
    <name>A0A5B0LLA7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007455</name>
        <name evidence="2" type="ORF">PGTUg99_003226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000518">
        <property type="protein sequence ID" value="KAA1063915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLA7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366001" evidence="1">
        <location>
            <begin position="20"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1063915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="57" mass="5981" checksum="06EE07E591EA4CE6" modified="2019-11-13" version="1">MQVCATLVLCVILFQAVISSPKPINTHAKLSGDDDGQCGDCGDGRPGCCWPGDRGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNR5</accession>
    <name>A0A5B0LNR5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleolar protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 4">Pumilio domain-containing protein NOP9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NOP9_1</name>
        <name evidence="6" type="synonym">NOP9_2</name>
        <name evidence="7" type="ORF">PGT21_008133</name>
        <name evidence="6" type="ORF">PGTUg99_008198</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000518">
        <property type="protein sequence ID" value="KAA1063942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNR5"/>
    <dbReference type="OrthoDB" id="392571at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="265"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1063942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1065706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="709" mass="79918" checksum="78EA9931E803B920" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPZ9</accession>
    <name>A0A5B0LPZ9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031784</name>
        <name evidence="3" type="ORF">PGTUg99_026823</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPZ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366025" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="59" mass="6246" checksum="DBAD24A3B3895F7E" modified="2019-11-13" version="1">MLASGLYTSLVLLLIGTFKLAGCAPGYSFTFSCSGLPEGIQQKCCDNGQWDSSRDGCHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQ72</accession>
    <name>A0A5B0LQ72_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017964</name>
        <name evidence="3" type="ORF">PGTUg99_021724</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000110">
        <property type="protein sequence ID" value="KAA1130362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQ72"/>
    <dbReference type="OrthoDB" id="2507904at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366026" description="Hydrophobin" evidence="1">
        <location>
            <begin position="22"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12968" checksum="6404959D9BDC5D8A" modified="2019-11-13" version="1">MNLSSLLGISMVLFSLQLQMAMVESMGCGNAGNDFKYAGCAKHLKKEAFPGGDPRYWSWMMDVIPPPWKTDHYDCKGTNYPYEVCCSIHVENIKNARDTRLAYLCRKPNGANLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQP9</accession>
    <name>A0A5B0LQP9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQP9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="262" mass="28839" checksum="1CAF6273EBA39D7A" modified="2019-11-13" version="1">MKNLQSSNIGHGGQAQVDSTSEAQATGTGKHSQQDAGPKNAHHQDIVPLPEDTEDVQAQKTVPAQGGDMSGTVGEREHLSLEEIRGTSSAPDKEDKVIAGCLTKDKQRDAYRNLLTDEEEPGPLLVVNKAPNVHSIPPIARRKTDRERLWDQVCKAKESKDEANADFLLRIYLSLPKDTDVLAVPVQLSNVRSSSSDAALPTQHSRSVDKTVNFIRGSVPNHFDIGFTPFFDKNIREFRGPLPLTIFNKEWQEDAVSFHSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSI3</accession>
    <name>A0A5B0LSI3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007923</name>
        <name evidence="2" type="ORF">PGTUg99_029614</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1067056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSI3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="95" mass="9452" checksum="6998821AC2C5669D" modified="2019-11-13" version="1">MVLDLVGLGKLGEGVGQGQVGNADAALGVHDAFLGQSDQLVGSFEVPSGGYQQTISTTAASASGAATPNPASASQCPRSILAPPQYTLTPIRSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSS6</accession>
    <name>A0A5B0LSS6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013429</name>
        <name evidence="1" type="ORF">PGTUg99_020605</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSS6"/>
    <dbReference type="OrthoDB" id="2510515at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="568" mass="65646" checksum="E820500AAE3368DA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LU26</accession>
    <name>A0A5B0LU26_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cutinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_011944</name>
        <name evidence="5" type="ORF">PGTUg99_026069</name>
    </gene>
    <organism evidence="5 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LU26"/>
    <dbReference type="OrthoDB" id="2586582at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000675">
        <property type="entry name" value="Cutinase/axe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33630:SF9">
        <property type="entry name" value="CUTINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33630">
        <property type="entry name" value="CUTINASE RV1984C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01083">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01110">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137103" description="Cutinase" evidence="4">
        <location>
            <begin position="25"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="262"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="296"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1067198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1071571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="37342" checksum="D5F3FB610897417E" modified="2019-11-13" version="1">MIFGNLISIGISLLFMNLQDRTYALTFSQNSHPNPLHSRQSGMGGLGSKGGGMGGLASKGAGGGEDSGGCKTYMMMGARGTTEAQGSSMAYTKMAKNVLQAVPGGGLMDIQYSSSAEYMKSPKDGAQTGLKYLKAQASKCPKMVFVLMGYSKGAMVQSQILAAKDLPPNKVVAVVLFGNPYFTAGAPQNKCDAKKGKGIAAAMMVKVPKDYTSLIWDCCLTGDMICQSSGGMTTHMKYGGKPASEAQAFIVGKLKGVKEGGGSGGVAAGTADKTSDTGAASAADGADSQADSGMGSKFGGGMGSKSGGGMGSKLGGGMGSKVGGGTGSGGDETGGKTAGKMGGKMSGLMGGGGDDKMSGSKMGGLGGKMGGMGGKMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVU1</accession>
    <name>A0A5B0LVU1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024429</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVU1"/>
    <dbReference type="OrthoDB" id="2501823at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="110" mass="12393" checksum="10CB62FF66B5363F" modified="2019-11-13" version="1">MDIAIRSLGNQLPYSGGHVRNSRAISKVLDFQIARLEAKIPAIEKALENMLNYCANCSVKERFDIIMRAGESGIIVDRFLDNLDKIGCLTKDQCEEEKKKLNTVKKPPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0F7</accession>
    <name>A0A5B0M0F7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006951</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0F7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="52" mass="6095" checksum="A4FFFC7A7B76A76B" modified="2019-11-13" version="1">MINFRRFLAYSELPKNELKRLGKTRCRSRKPGVKNRVWIAEKGANSCKSLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2C4</accession>
    <name>A0A5B0M2C4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prolyl 4-hydroxylase alpha subunit Fe(2+) 2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010861</name>
        <name evidence="3" type="ORF">PGTUg99_002793</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000484">
        <property type="protein sequence ID" value="KAA1070110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2C4"/>
    <dbReference type="OrthoDB" id="2495224at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099:SF7">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="684"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="920"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="940" mass="105762" checksum="0E0A7D6F235B2C5A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M569</accession>
    <name>A0A5B0M569_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M569"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="310" mass="32385" checksum="AF20B3EA5D1FA8B0" modified="2019-11-13" version="1">MSRTRSHRPALVEAKDLTGAPRGRHSAKISPSVSSASSGGEAKSRVLPSSGEDKGRGLESCSPQIGIGTPNRSSVLDHSGGVPEDLQLGPEVGVRRVPEYSEGPNIPEGDRSGSNADSSSRSPNATARGNSVSASRSTDGDASSDVLSSKRLLLPVSSDEYKPAESVLAPTGLQSELLDVPTRVLPTSTVGKQPSSPRGTVGPPSRDAKEGTRPSSEEQSQPSSQQKGKGTKLGTSSQGVDKRTPTPMAHANLHASCRLACAPARHCRMACRQARHSPAGGISCRLACLPARPIWVLNAYLYAFRTWAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7P8</accession>
    <name>A0A5B0M7P8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7P8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="191" mass="21209" checksum="9F8D4CFC65CF6EFC" modified="2019-11-13" version="1">MGAVIQASYDSALPHLTQHQASPSDLTKIIAQSCGETKPQDGNNVEPPANSAGKGLKIRIRRIRSDMPTQANPRVLDAKTEKPTAQRGRPKARKLVDKTFKSEPLTDDDWARISATYWNNRPKETDREMLALHSKREATFGSSAVLQAGRDPHLPETIHDNSQGTEEHQLTLHTSAQMGSQALRRSSRNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M983</accession>
    <name>A0A5B0M983_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TIM10_2</name>
        <name evidence="16" type="synonym">TIM10_1</name>
        <name evidence="16" type="ORF">PGT21_025296</name>
        <name evidence="15" type="ORF">PGT21_025323</name>
        <name evidence="17" type="ORF">PGTUg99_005246</name>
    </gene>
    <organism evidence="15 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="17 19">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000247">
        <property type="protein sequence ID" value="KAA1118546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M983"/>
    <dbReference type="OrthoDB" id="274922at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038:SF16">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="14">
        <location>
            <begin position="22"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1073777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1076958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1118546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="93" mass="9957" checksum="3FF01FD6B08002E6" modified="2019-11-13" version="1">MSIFGRSSSSAPAPSAPSNIDAAATELEMVSDLFNRLVRSCHGKCIGNRYLEADLTKGESTCIDRCVDKFFNVNTKVGERIQAKGAASQLSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9T7</accession>
    <name>A0A5B0M9T7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9T7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="130" mass="14028" checksum="CE197BD68D6C5B35" modified="2019-11-13" version="1">MTGEVRLCGCKSTSSSANNPSQNNLNHQQLISERLRLNLERTRLTLALGQIRYTNISLIVLMGRIRGQPSLTTKQKACIAGMVEAGISHGRVARIVGSGQTTVSSIVSRARTCGTVETAEKSGLDRIAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAT6</accession>
    <name>A0A5B0MAT6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035421</name>
        <name evidence="1" type="ORF">PGTUg99_025759</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1073078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAT6"/>
    <dbReference type="OrthoDB" id="2507933at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
    </protein>
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    </gene>
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        </lineage>
    </organism>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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            <end position="214"/>
        </location>
    </feature>
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            <end position="296"/>
        </location>
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            <end position="708"/>
        </location>
    </feature>
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            <end position="732"/>
        </location>
    </feature>
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            <end position="476"/>
        </location>
    </feature>
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            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
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            <end position="58"/>
        </location>
    </feature>
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            <end position="68"/>
        </location>
    </feature>
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            <end position="80"/>
        </location>
    </feature>
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            <end position="100"/>
        </location>
    </feature>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="EMBL" id="KAA1073786.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="747" mass="80922" checksum="9A03DE5BDD4EE799" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:endocytosis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF1">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="406"/>
        </location>
    </feature>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
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            <end position="798"/>
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    </feature>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="858" mass="95657" checksum="53A53C0B4032B9E6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ME87</accession>
    <name>A0A5B0ME87_PUCGR</name>
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        <recommendedName>
            <fullName evidence="3">cellulase</fullName>
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        </recommendedName>
    </protein>
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    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
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        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    <dbReference type="OrthoDB" id="5823761at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142:SF1">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023060594" description="cellulase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="12">
        <location>
            <begin position="67"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1074398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="358" mass="39738" checksum="75FC7724229D9B47" modified="2019-11-13" version="1">MLLSSYKLFSLLPMTIAFGQGVKALNLFRGPSQQDVKRWAGVNLAGLEFGMQNTGTMNQDPMEPPPISQIQHFRAENVTAFRIPISWQRMQPKIMGPLDPDSLQLLSKYVNSALACKASVIIDLHNYARRDGKVIGEAEDLPASSLVDFWTRIAQKFGEFPEVGFGIMNEPHDVKLDIWIETLQAVVTAIRKAGATNKIILPADNWSHLQTFATSYNAGMSKIHNPDGSCTGLIFEIHQYFDSDGSGTSASCPVPHTKELQEVVALLAKDDRQAMITEFGGGHNPDCPSVLADFVEEAHKSFPQILGYFLWGAGAFHQDYTLVVTVEKDGEWVDQSNFLAIQKFMDPGPKRNQIGWMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFA9</accession>
    <name>A0A5B0MFA9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016132</name>
        <name evidence="4" type="ORF">PGTUg99_013281</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000271">
        <property type="protein sequence ID" value="KAA1115881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFA9"/>
    <dbReference type="OrthoDB" id="10330172at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366149" description="Hydrophobin" evidence="2">
        <location>
            <begin position="20"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1115881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="16991" checksum="1299F0A185135F23" modified="2019-11-13" version="1">MLFPKQLFLLAFLYGSSISARSLQKRLLKSGKCDLKITAENNVFASLDKNLAKVVQPRIEGNKFLKNLCGALNQANCGNVDKKDVDACNNLIKKATTDALGAAIATKWNKECCSQGGGGGQQKKKQAGNGTQKKNGGGGGGGGNPKNGGNNGGQTQKKKGNPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MH09</accession>
    <name>A0A5B0MH09_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Serine/threonine-protein kinase M1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">MEC1_9</name>
        <name evidence="2" type="synonym">MEC1_7</name>
        <name evidence="2" type="ORF">PGT21_035970</name>
        <name evidence="1" type="ORF">PGTUg99_037240</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MH09"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="115" mass="13339" checksum="53BDFFA570945D2C" modified="2019-11-13" version="1">MALHKQKELSDATKNVLQFTFGQEFCRTPVLLIRRFPLAKDHQDLIERAQGSTSRTQAEGENTSPAVLEEELLIWLLIRMLYCAALIRDSPMEHREETLPMSWFQIQPINRVSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHG7</accession>
    <name>A0A5B0MHG7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHG7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="308" mass="35977" checksum="73BE853247ADA590" modified="2019-11-13" version="1">MAKLSHQEWGEDDEFGDPQVELIHYFSLNAKEKEKIAPNLATSLVQRFQEEDPINFTEALNDAGESNKIFSSAALQKSNEKLRQSLTNDQKFETIVNAKRWPLSGDYLPKSTLEYFKHQLKKLNEDQTAKQSAQSVHPKLPVAVYFFKKMPDYQMVRILENTPNKGVVRFNVMYNKFWKLVLATTYIHLKVSQEKEMTKLEYQQSRGRLFEWIQNMILKESDNTGIPLIGLIKNDRNKAPWEDQNGKEHKSFRIDEAILIEYLSNEVNPESLKKASTLLYTHWNQLDELDDRTSGNRFQNKKLKTKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJZ9</accession>
    <name>A0A5B0MJZ9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJZ9"/>
    <dbReference type="OrthoDB" id="2505969at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="465"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="61850" checksum="F505E65C4349A794" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKI4</accession>
    <name>A0A5B0MKI4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DnaJ-protein scj1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SCJ1_2</name>
        <name evidence="11" type="ORF">PGT21_006970</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKI4"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000013">
        <property type="entry name" value="DnaJ subfamily B member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000002">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="29"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="10">
        <location>
            <begin position="155"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="155"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1077415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="429" mass="48562" checksum="FAE70406ED8BC856" modified="2019-11-13" version="1">MANSNYYHLEILSICLIILITASDIFALDPYKILGVSRKAESVDIRRAYRKLSKRWHPDKNPNNEEAHQKFLEISESWEILSDPETREIFDKRGEEGLKRHREGGDQSDGFDFFSQFFGGGGGGGRSSNSKKNAKKKGPTMATDMEVELEDIYIGRSIDFEISRRVLCPACKGNGARKETDIVECEKCQGQGVRIIRHQLGPGIFQQMQMQCDACSGRGQTIKHKCTQCHGERTVEEVNSLTLDIDRGSPDGHEEVFEGEGDEGPGYSAGDVLLRIRIKKQSDGGFRRLEENLYWKEVLSLDEALLGFTRKIKHLDGHDLTVSRQAVTQPGFVEVIEGEGMPRHQALGYGNLFIEFAVVFPMEVSGPFRAGQHLFFLRFRRFDQLILAEVVFLVVGLEKVFKPYRTLGDLANEEKIEKVKSKDEDHQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLZ2</accession>
    <name>A0A5B0MLZ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLZ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="99" mass="11744" checksum="4FC1A858CFF21F4E" modified="2019-11-13" version="1">MTISSHSLRLKTKHCFLLIKNDISRNLIWPTPHIQTLKNLKASHIYETLTSGPQLREDPFLEEGKLSLTHFEVELELPNFFRLDDKDFLRLFSHSLFYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNB6</accession>
    <name>A0A5B0MNB6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006038</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNB6"/>
    <dbReference type="OrthoDB" id="2505909at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48159:SF1">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GIANT PROTEIN ANTIGEN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48159">
        <property type="entry name" value="MULE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="347" mass="39437" checksum="B5E38F4E59670E2E" modified="2019-11-13" version="1">MAHFITSPTPFTYPLIQNIYDVVPRPHTQDVPATVGVSSAPNSLRLVRNIGGPSPTFPTHPYLFTIPGSSKSEAEEFVKAMTATIRWNFQRGAPTPPDKLVKSHGSGRRPEAFFKLEYQCPSSGYCKGNPDSRKKNHKSARCGCKARFSVTHHIQSNTLRVSWHWQHNHDLNTHRQMVITRPPVIVDQWIKDRVDSGLAWKEIYDLTQVDDVLDLRSSTVKPEAHGVTYDRVRYLIQTRRTIPSQLNRDPLQSLSIWHSDLIARNWNTHVNIFDSANFIFAFQSPWQRKMLVEHGRGMLMLDATHNSVNNCFLSDGRKVSLYTFLIRDPIVGKGLPIAWAFTASAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNY0</accession>
    <name>A0A5B0MNY0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNY0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="189" mass="19581" checksum="F5802ED4EF002568" modified="2019-11-13" version="1">MKFSTSVSQVKSSSASLGFRSVSSSSSWIQLIQLIQLLEPAHPAQPALQLSQLFQVSELAQPAQPAQSAWAALGASSASSVSSVSSPSQLSQLLEPANPAQEGLGSSSVSSLSQLTPGASSVSYPGQIIQLRKVLKPAQAAHPDLEASPSSSVSSVSPVGSAPLQIFQPSWHPSLIEMVALDPDIRAVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNY8</accession>
    <name>A0A5B0MNY8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNY8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="109" mass="12082" checksum="8A79551C765B3F5D" modified="2019-11-13" version="1">MLTLSAPLTRGDCDSDSQLRQRGWYGTSNSSQPSPNLNNHCPICIVLLSLLVLILHRLKDFQVNQCCPDGRARLQTIPVCISRDQPNALTGEPMLPQWRTAPHPTSHAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRF4</accession>
    <name>A0A5B0MRF4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRF4"/>
    <dbReference type="OrthoDB" id="10585694at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="453" mass="50442" checksum="732276DD25327284" modified="2019-11-13" version="1">MSPPLLFSSAPDHSLILNLVSLPNPSFERTINMDPTQSSSSPVCSPDDHSREASDLKVRATTGELIRRLEGMQRRIDQKREGMEGRTEGEESLIDRLKTDLLPALDEGLGQLPGQLLVQPATRSPSRRGFRPASEIEAGLATIVRTIGDTVEKIVRIVGRSTAALPGRRLSSDDSSLPQEEIDMRAFRREEIRGQTTALIHRLLEAFKFLHYVASSTPIGFQGQRSPSEESSWLEEGGEMEEWVEMNEGVREAVCSMRGSMARSDLAVLQARWQAMAGMVERTLARLSYLAGQAGGRQSHQALLVRAAVPIVKLSRVFFHKLADPTSVFLEDIAPHQLLESLLPSTLQLADSVNRLDFSIKLLPPPPPNLPPLHISSSTPPQWLQNTAQSFLHTLLLLLHSFRSLSLNHHNHPPPLMNSRQNSHLLWLLTCQTLVDRAFQNFLHVYLATYNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSC7</accession>
    <name>A0A5B0MSC7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032076</name>
        <name evidence="1" type="ORF">PGTUg99_003146</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000444">
        <property type="protein sequence ID" value="KAA1079024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSC7"/>
    <dbReference type="OrthoDB" id="10300969at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="86" mass="9663" checksum="F349B964A7745046" modified="2019-11-13" version="1">MDMDYKYFLARQTEKGTAYLCVLCMGKTTANQRQNCCNDRHQILVQAEKDRVDCIASWRAENQASGELASVVDEGITQLDVVVSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUL7</accession>
    <name>A0A5B0MUL7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_007119</name>
        <name evidence="10" type="ORF">PGTUg99_027922</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUL7"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF4">
        <property type="entry name" value="ARGININE PERMEASE CAN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="63"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1080272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1104002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="567" mass="62697" checksum="8E9755B64F9B844A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWP7</accession>
    <name>A0A5B0MWP7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_027191</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">21-0</strain>
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    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAA1133706.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="497" mass="57361" checksum="26E87CB35F0212F8" modified="2019-11-13" version="1">MKGTRKRVPNHADEEENLPKRISSLDTKYLDPWSTRDPQWQDSGGHPGVFVNPRRVRMANQDTSASLLQNPNPSHREHSNLEGLEHSDQLGHPGNKVTRDLLSRFDSHAVQPFEKEKSESSTGSHGKEILNYHPRIIPVVSSNRPPWSDLPEIALVRKYYSCQTLDWNLSEVNEEAALGAYAMVTEFQDKIPAGTTFSHLGRLQNSHMIFIPFIYKLMIKPLETHQWPTILRVWRSIWYFSEKIPRKSALSADQVLWKFLWISDLILESTIPQLFENSKVTITTSGKEVLNSGTSTAEAEVVRILSGSRGDWRFNRSENDSLKTLSIFLQQACSNTEKNLEGSLNNLVLERLESTARLITSAISTTLDKAKGMDPQYQQFFLEYFRDVYLRRKPYDPQWFGNILSHPFQKRIDEILCKRGFDKNLMLIQSYHPASNALPAINFLKKFDQYVLRKPGAFRIQEQVEAPFLPSKISQFFANQFKKYRQSDQPQIVNENY</sequence>
</entry>
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                <person name="Sperschneider J."/>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
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                <person name="Upadhyaya N.M."/>
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                <person name="Schwessinger B."/>
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                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3J2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="219" mass="23861" checksum="1641AD012E1ECDC8" modified="2019-11-13" version="1">MRKNRVFLLHRGCNWEKAGYLWDGTGPEPSPLGVRHPLVALISPGRFVANTGMTSRGIHPMGVFSHHQWPKDAPRLPSGSTADTRNQTPQGNPCMSQGYSPNPHTYGSRRRSLLDERTSPPNHLPCQQTSPTAVQEENRLKAEYGRFRAIFVPAGSQAAQRSLKASSVDQVSASSRTLPLTSNPTMFPLNPRASKTPVTPPAHQTHPATAPVLVRPGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3W6</accession>
    <name>A0A5B0N3W6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_011685</name>
        <name evidence="5" type="ORF">PGTUg99_019207</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1066956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3W6"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004297">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF6">
        <property type="entry name" value="AFG1-LIKE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="41"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="518"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1066956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1083921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="63677" checksum="2A6F0F1A3163BD09" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5K0</accession>
    <name>A0A5B0N5K0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DUF221-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_006759</name>
        <name evidence="13" type="ORF">PGTUg99_006064</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000214">
        <property type="protein sequence ID" value="KAA1122869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5K0"/>
    <dbReference type="OrthoDB" id="2150324at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF149">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G11660)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="434"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="481"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="587"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="612"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="650"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="31"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="210"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="386"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="763"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="840"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="934"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1083784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1122869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="957" mass="107498" checksum="A5C5312E4A813880" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N759</accession>
    <name>A0A5B0N759_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N759"/>
    <dbReference type="OrthoDB" id="10278993at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="14148" checksum="32CBC3A05D632A2E" modified="2019-11-13" version="1">MPPNSEGLQQFAEEDVSLVRRIGKLMCHRLADTDHTPPSRTKPCHLAENISLPSWYGRYQLDDEDSSRSAETVLSQFAEKEIRWTTPDGVIKLGAATPKNYRHGLWRRFIVTILTKLSGSVIPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBJ9</accession>
    <name>A0A5B0NBJ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBJ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="124" mass="13986" checksum="1394C36D4EC0EBA4" modified="2019-11-13" version="1">MSLKAPDNRIYSDESTPICSFRSSSIFLGNDLFIPNRFFCFDYSLFITHSKRTDTARSLNNIRIHSFLVVLSSTPTESTCSAFGSTSICFQELLPVLKSPSNTPIATVVQKLLIDQRPVIQEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBR4</accession>
    <name>A0A5B0NBR4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBR4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="52" mass="5546" checksum="D82F6DA897A5AD0D" modified="2019-11-13" version="1">MVNGTFASEDFIFTLKAKVIKCTRERVNTITSASANGVGREIGGTVISPDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEF0</accession>
    <name>A0A5B0NEF0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEF0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="122" mass="13092" checksum="0FBFACB696D7E267" modified="2019-11-13" version="1">MTGGPFSAPAVLQFNPGKITGGIQTRSRLLTSAAHLPAALCPCSSRTLNVNTKNGSKKSKENSQIQSPFCNPDANINSKHSKEKKSKTKKKNKSTKVSPTSLPAKDSNINNDTTPKAKKPMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEK9</accession>
    <name>A0A5B0NEK9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Histone-lysine N-methyltransferase set9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SET9_6</name>
        <name evidence="2" type="ORF">PGT21_024053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEK9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="214" mass="23141" checksum="5783EC59437F4D02" modified="2019-11-13" version="1">MGGDNPLRISAIRWRISARASGYPPGDADSGADVLSPPKSRRISGYPLGYPRPMAGWKGFLPAGNSLRSPGSNRRIEKFNQSNHAHSTARKCGIRFCETDRYLVGQVASTTGSPSTCTSSISPKLHSRPRRAVVKERQPKPASRPRRKPRSFPAALDTPSEHSGSLSPVPTPRLIPSHRSNDNIPRDIQPKDDPASSAKSPSTNGHRLLVLSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFE0</accession>
    <name>A0A5B0NFE0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011488</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFE0"/>
    <dbReference type="OrthoDB" id="10277996at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023002912" evidence="2">
        <location>
            <begin position="27"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="414" mass="46795" checksum="F36A8E89727ED541" modified="2019-11-13" version="1">MATSHWSHSFIFLLIFYLCLWTNVDGQEAKPGILDGGMTFAHKTQAEKSAREGCDVPMAPMKSSLMIETPKAEPVPSAKENPVSEKTNRSREPEEIHRNPAQLDTSENRKRRPRRTGFLAWTRHHLNRLWNWLCRLFNKSTSKDKRTEKWAPQADHEMLQNQKSDTLENIHENGPAPPDGANLENASAGHAAQVSTGEAHSEGDGSETVTPGPGKVKPDPGKNQPGPQKTPPESDPPKVEHHVEHVKNDPEKVKGEAYVFWELTAKKNGISPNAERLALTKTVMDLLFEAIRHGIDSKTAILTSIDPIVKEMGFIKKRMVPKGCTRLCKKIEENIGRQKALIREIIGTDHHRSWAQLMQDTNKSSQFEDAFVSTFRECDGFIKSLQDWKYHNESPKRFLLHIEEYTSKLRAKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NL62</accession>
    <name>A0A5B0NL62_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019831</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NL62"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="11220" checksum="91880C20D4F0F728" modified="2019-11-13" version="1">MPSYKIVATILLENFKKILVFKLWTSQGNLQTNRGSQVDQKKARFDFESTQLIGPSSYAQEDELQTPLVLRTVPLESTRGVDQGSAPSTGAKRAGDPSIGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNY3</accession>
    <name>A0A5B0NNY3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sn-1-specific diacylglycerol lipase</fullName>
            <ecNumber evidence="14">3.1.1.116</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGT21_017961</name>
        <name evidence="18" type="ORF">PGTUg99_011967</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="18 20">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17389"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.116"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33276"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.116" evidence="14"/>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNY3"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046340">
        <property type="term" value="P:diacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052214">
        <property type="entry name" value="DAG_Lipase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792:SF8">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="16">
        <location>
            <begin position="467"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="222"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="418"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="503"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="277"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1070615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1089790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="827" mass="92351" checksum="11E601D7E79C8917" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NT62</accession>
    <name>A0A5B0NT62_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Riboflavin synthase alpha chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RIB5_1</name>
        <name evidence="5" type="ORF">PGT21_012984</name>
        <name evidence="6" type="ORF">PGTUg99_014977</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000381">
        <property type="protein sequence ID" value="KAA1091882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NT62"/>
    <dbReference type="OMA" id="HFVTGHV"/>
    <dbReference type="OrthoDB" id="10258924at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000014">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006767">
        <property type="entry name" value="PRK09289.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098:SF0">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Lumazine-binding" evidence="4">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="4">
        <location>
            <begin position="125"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="125"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1089286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1091882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="299" mass="32941" checksum="ABB0639C9E98580B" modified="2019-11-13" version="1">MFTGLIERLGRVKSHSSDHSTITIQVLRREELTKYQTSLNPDPTSTTAKDDQGVLEFFNDMKEGDSISVDGVCLTVVEFSVDESSFDLNLAPETLSRSNLGSLKVDDLVNVERALSPSTRFGGHFVQGHVDRTGTIVSKTKDGDSIRMTIEFEEDSCESSKDGGVEEGTTKKATEYSKFLIPKGYITIDGISLTITGIENKESIRPGTTLKNRSTRVSIMLIPHTQLNVTLSLKSIMSTVNLEFDMLVKTIINVIEYSLSDQLDRIINQALRSQLAQLGIQPSSSPTQDPIPPQPLELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVS4</accession>
    <name>A0A5B0NVS4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1279 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_008766</name>
        <name evidence="5" type="ORF">PGTUg99_023252</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVS4"/>
    <dbReference type="OrthoDB" id="426386at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045866">
        <property type="entry name" value="FAM210A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009688">
        <property type="entry name" value="FAM210A/B-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377:SF0">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06916">
        <property type="entry name" value="FAM210A-B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1279" evidence="3">
        <location>
            <begin position="109"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1092624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1093721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="230" mass="25727" checksum="9372E0736C86F475" modified="2019-11-13" version="1">MVPAALFQKDSLNQKSYEYQHIHRDPSDSSRLRASKFSNPTSVLSSSSPSSVSANTSIDQHPKTLPSDYSLGNLFCKSTTAQDDLHQKGEKSPSFRTEEPTARVGLFGKIRQLTKLYGSAALIVYGLIGGVDFGFSFMMIYLIGAEHVRKAEDWVREQVHWKRAHDSLSTTSVPGGSEIDSDMLWTSAVVAYTIHKTILLPLRILLTAWITPPIVRHLRKRGWKIGRNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZE5</accession>
    <name>A0A5B0NZE5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dihydrolipoyllysine-residue succinyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">KGD2_1</name>
        <name evidence="14" type="ORF">PGT21_009787</name>
        <name evidence="13" type="ORF">PGTUg99_032742</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="13 16">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="4"/>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZE5"/>
    <dbReference type="OrthoDB" id="5391403at2759"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000007">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004309">
        <property type="entry name" value="PRK05704.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="12">
        <location>
            <begin position="87"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="160"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1083418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1094102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="519" mass="57169" checksum="06B3561235E0D7A5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0N9</accession>
    <name>A0A5B0P0N9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0N9"/>
    <dbReference type="OrthoDB" id="2506684at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="367" mass="39987" checksum="15984542B5D0868F" modified="2019-11-13" version="1">MNQALAECRDEVKNRYPTNTFAQIISSNPSLPLNSATIYTPPYLNLAYSQSSNLSWLIATPLIPSDNSQILCAPFIAALRTKNPVIAPGSTYGQLPADSGVTFNDSATGQEISMKVQLFGYGSASMALIRDHTYLLSGRLISPNLKTPPVLYYDQDLTFPMGLTANLPIALSNKTAVWGFGLVISKHERDDTSGGQSSFRSLFVVMKHTDYDNQSKNQVSFNVSYKIPGNRNLAKTYGLFQPGREMLLSGTLTGYDKTQRMLQVQVLSVSLSSGPEPVMLSQPPTDQTHNNTRKHYQISFDSDDDCAPEAAANGICSSAQATVSPGSDKTDAVTEPHLPEPQPKRKYTRKGKNIAPDTPVIDSPNMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1S0</accession>
    <name>A0A5B0P1S0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1S0"/>
    <dbReference type="OrthoDB" id="2537769at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012808">
        <property type="entry name" value="CHP02453"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02453">
        <property type="entry name" value="TIGR02453 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36452">
        <property type="entry name" value="CHROMOSOME 12, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36452:SF1">
        <property type="entry name" value="DUF2461 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09365">
        <property type="entry name" value="DUF2461"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="414" mass="46057" checksum="2C1ECCBDC9C75F74" modified="2019-11-13" version="1">MAARTSRPRSKKLKTKHAPTSNSEDEDKKPIGRPARRAKKKVVAKKEESPASASSLSSAESDAKKAQEKEFIPSDDPDEGSPELDSDHIDSDDGDDVPAKTKSKKRKSTATGGSTTGNPKSVVVKNIAPGPTKDQLVSPGLIAGFTMDLLENLAKPECNDREWLIVNDNTYRKALENWKDFVDLLCPKIVECDWTIPQLPAKDLVYRLHRDVRFSSDKTPYKKYFSAGFSRTGRKGPWALYYLQIEPGDKSLVAGGIWCPDKNQLATIRNSIMRNPKPLRDVINHKEFTEMFGKPSGKPGPSERKNIFGHKDELKNCPKMNGVDKSHPDLDLLKLRSIAATKSPLLTSRPLLITRASSLKNAELNEIKQYRFPDEVVMSSSFMDEIAKAVKALSPLVQCLNEMILPQSDDEEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5A4</accession>
    <name>A0A5B0P5A4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5A4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="135" mass="15664" checksum="0965446560B23F42" modified="2019-11-13" version="1">MVLQPQLISTSLTTSSRANGADQSEKAHHKSNHPATKTALFSHHNNTFEDNHHDTGQLPGLETELEEEDNHQEPDIWVTLAEDKQLDHIDLMVQMSNKQHHQRAWEFNWNVLMGPLHSICMVQRIITDNWRFQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5D4</accession>
    <name>A0A5B0P5D4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008680</name>
        <name evidence="3" type="ORF">PGTUg99_016791</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000343">
        <property type="protein sequence ID" value="KAA1099091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5D4"/>
    <dbReference type="OrthoDB" id="2514537at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="67" mass="7518" checksum="D4D4AAC9A4D67BCB" modified="2019-11-13" version="1">MSAVLIGIEVGCRIRELGSERRTRNEAPAAQRSRLRPQFSSTSRDPNQNSTPNDRKTPWAPPKGLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5R6</accession>
    <name>A0A5B0P5R6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5R6"/>
    <dbReference type="OrthoDB" id="10563991at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="131" mass="14028" checksum="F7F4CDB657C10B62" modified="2019-11-13" version="1">MANLFNSVLGASTAVNPGDRTLCPIELPGTAGPSNLLRTNRANTAPASGSHIGNHRQDEVPPIIPVFQHHRPDPPPHQPNAHAAETEDERDFRLSELKLKANKLVLSTVASIVTGMKKEDALQPDGSNFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P974</accession>
    <name>A0A5B0P974_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012989</name>
        <name evidence="3" type="ORF">PGTUg99_016170</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P974"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="56" mass="6194" checksum="D9920F6B0533CCD7" modified="2019-11-13" version="1">MNSPNSKRSKLDSSLQQQQSNSLALPSHFWKPNQNNTQNNQSTNKLGLAGLSNPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9L3</accession>
    <name>A0A5B0P9L3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9L3"/>
    <dbReference type="OrthoDB" id="3265969at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09276">
        <property type="entry name" value="Rnase_HI_RT_non_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="75"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="501" mass="55523" checksum="14836B2E6AD395FF" modified="2019-11-13" version="1">MPHYLNWTCILDSNHSPANYSDKHRICFFLDKSFASEEIHLIKDGNRILSAIDLIIDNDNIRKLRLINLYNPPRTFEGIKILSDWLDQHNNRHKGTASAALLNNSISFACRINVAEKASAYEAEVQAINIGLDIISNEAEKNNLPPFNNVNIFSDNQATLQVIANPPLSKSNQSTFIQIFDKLNILITDFHFSISLLILWCPAHVGIPENKKVNQLAKEATEGNTLFDLEQQSRTLSNIQQIINSKFSFVKKKDPIIRNHISLSNIPIKIFNSLNRLERRFSSTIYQLRSGHSPLNDFLFHIDKIDSPDCNQSKEDNSARPGSMEDFPSVLQPLDGQRVSVADTRYPLADTRQCQRIPANGCGFGCGWPLFRKKLAGIRVSQRIPAAGRGDGRLEGSPSGRRGTSTSSTGRTPFQSTRYKYLAGWKETLPAGPLEGGYPRIPARIPAAADGYPPAGADADSDAQMAGKSSRISASARISGCQSTISTPIYPIKQLLEPPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAG9</accession>
    <name>A0A5B0PAG9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAG9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="11290" checksum="C3B0313DC2D133EA" modified="2019-11-13" version="1">MDRPHAKKRTFDHSPLAEPIDRSISTDCTTKKKKTKKAKKTYPLDERSIIKPDGLRLQPTEVQQVRGGSKGFTQPPSASNPLPPSASDQARTNHEGPVISCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBM6</accession>
    <name>A0A5B0PBM6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Oxysterol binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">KES1_1</name>
        <name evidence="4" type="ORF">PGT21_031888</name>
        <name evidence="3" type="ORF">PGTUg99_010306</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1098975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBM6"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120009">
        <property type="term" value="P:intermembrane lipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000010">
        <property type="entry name" value="Oxysterol-binding protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000018">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.15, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF184">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN HOMOLOG 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="408" mass="46047" checksum="C271E2E1CD3A094D" modified="2019-11-13" version="1">MATEEEAVVPTNQRSSWTSFLKSIATFSGDLSSLTAPSFILSPTSLCEFPSYWGEPHREFAAISTGKNPEERMILTLRWFISTLKGQFTRRETQTGTEKKPLNPILGELFLGSWNSGPSTDENSTGELRLWTEQVSHHPPVSAYCLINAKAGVSYQGHCGQKTSFSGKSVIVKQVGHGLLRIKLPDNTIESYLITLPKLRIEGLWMASPYIELTENSHIQSSSGYHTVLEFKGKGYFSGKAHSFTAKIYSQPKITSGVSPVYEIEGQWHGFSKFVKQVPKWWQGSGEKFLDQTEEKLDEITVQDISKQGPLESRRVWKDVADGIRSGDFEAASSAKSKLENEQRAKRKEELANGTPWQMSHFDRVDSDEEYARLAEMCGHSPPTEEAYYFKFENEYIQETLSKQQQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBS2</accession>
    <name>A0A5B0PBS2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="2">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_027813</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBS2"/>
    <dbReference type="OrthoDB" id="10264196at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06822">
        <property type="entry name" value="PLPDE_III_YBL036c_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000042">
        <property type="entry name" value="Pyridoxal phosphate homeostasis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00044">
        <property type="entry name" value="YggS family pyridoxal phosphate-dependent enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PROLINE SYNTHETASE CO-TRANSCRIBED BACTERIAL HOMOLOG PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146:SF14">
        <property type="entry name" value="PYRIDOXAL PHOSPHATE HOMEOSTASIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01211">
        <property type="entry name" value="UPF0001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine racemase N-terminal" evidence="4">
        <location>
            <begin position="75"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1098070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="313" mass="34330" checksum="8F03E3B48D11F226" modified="2019-11-13" version="1">MNIAVTCSNGLLGQSKIPREHTVSQTFSMFLNRLAATTRTHRPYSSATRMALQAETKTADNPDERARDLLNCFNNINQQVHQAASGREVRLVAVSKLKPASDILTLYRTTGHLHFGENYVSELQEKVKALPGDIKWHFIGALQSNKCKILGAIPNLFAVETVDSLHKAQLLEKSRSALASSVQVNPLEVYLQVNTSEEASKAGFISPSNEPILSSNLHSTAKYIKEECRWLKLAGLMTIGSIGQSKSDQGTNKDFERLVQLRDQLSESLGGLELGLSMGMSADFALAIKMGSDNVRVGSSIFGERPPFKKPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFU8</accession>
    <name>A0A5B0PFU8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mitochondrial ribosomal large subunit component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MRPL16_3</name>
        <name evidence="6" type="synonym">MRPL16_2</name>
        <name evidence="5" type="ORF">PGT21_023838</name>
        <name evidence="6" type="ORF">PGTUg99_021234</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000208">
        <property type="protein sequence ID" value="KAA1123496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFU8"/>
    <dbReference type="OrthoDB" id="268521at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000012">
        <property type="entry name" value="Unplaced genomic scaffold supercont2.7, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_uL16_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_uL16_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220:SF13">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1099886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1123496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="233" mass="25805" checksum="7B05A598845A0099" modified="2019-11-13" version="1">MFNFRTNNTPLQIARRMNPIFNPQQQQRNRSDLAPKKFKYRKAFKGSTPIRTGGSTVATTLEHGQFGLRVLEPCRLSAKTLQSCETAIKRAIKPTKGAQCYLRVFPDLPVCVKGNETRMGKGKGPFEYWACRAPVGRVLFEVGGGGIQKEMAFAALQHAKPRLPVKSEFIHLDSKARLGGILVEKPMDTKKRIRLSSSSSSSSALNDSPTAIISKLLDQVRLSRSSSSLTKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIH8</accession>
    <name>A0A5B0PIH8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIH8"/>
    <dbReference type="OrthoDB" id="2509839at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="805" mass="93072" checksum="4B0E4610396F076D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJL3</accession>
    <name>A0A5B0PJL3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJL3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="56" mass="6242" checksum="21DC35D0C6E3E1A6" modified="2019-11-13" version="1">MTADSTPPLPRKQTKSQQQQQTSSSSSTCHRPYVCQIGLFKLPLKALESRPLVNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJW4</accession>
    <name>A0A5B0PJW4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ATP3_2</name>
        <name evidence="12" type="ORF">PGT21_001651</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJW4"/>
    <dbReference type="OMA" id="MQITSAM"/>
    <dbReference type="OrthoDB" id="239812at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039089">
        <property type="entry name" value="ATP_synthase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9 11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="4 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1100924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="307" mass="33537" checksum="4B863629139ACEE7" modified="2019-11-13" version="1">MSSTLITRPLISQRAARLIASPHAARSFGSTPTTNETLKEIETRLKSVRNIGKITKSMKMIATTKLNKAQRAMNDAKAYGEANAVVFKESSLSEELASGPDSKKTLFVVVSSDKGLCGGIHSSVSKRTRAELGENSETQIVVVGDKPKTQLSRIVPKNIVLSFNQIGKQVPNFADACVIADLIEEKKLDFDKVKLIHNKWISALSYESTVVEVYNADTLKLAPKFAAYEVEDDQLIQDLSSFAMTNAIYTALVEGYATEISARRNAMDNATSNSQTVIGGLQMKFNRMRQSQITNDLVDIITGANAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKT0</accession>
    <name>A0A5B0PKT0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKT0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1102197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="8843" checksum="B6C631AC89B0B2CA" modified="2019-11-13" version="1">MYCIGHTYDSTIGRSSTGSIIHPRVIDRILDHLRGELADSFSVLDAKWPPSFEKEGCRSRNFSTIRSSDNTRPTLLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLE6</accession>
    <name>A0A5B0PLE6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylglyoxal reductase (NADPH-dependent) gre2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GRE2_1</name>
        <name evidence="4" type="ORF">PGT21_018372</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLE6"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF579">
        <property type="entry name" value="3-BETA HYDROXYSTEROID DEHYDROGENASE_ISOMERASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_3G02250)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1101390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="347" mass="37874" checksum="DC93CEAB496177D9" modified="2019-11-13" version="1">MAAKENDTQPQRVLITGISGFVGAHVAKSFLDHGWEVIGTVRSAQKGNSLLALPAFSQPAKDGKIKFTVVEDISKADFNQVLKGVDALAHTASPFHFDGKTFSDYGTGIYFSYIHHIYAAQACPQIKAVVVTSSFVSVVDHTLPPEKQGGKVYSEADWLPITYEAADASSDSALWYCASKKLAEEAAWAIKKEGANWTLATICPPMIFGPVIHTGNLLGLNESSRQLYDLFSGKTGGEVPPTRFPCLSDVRDVAEAHYQAISRMASGRFIVASGVYDNQRIVDTIRKSFPAASDQVPNGRPGKHYRPEEVYALDSSKAKRELGIRIRQFDEIIVDTVQNYIDLGAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLW8</accession>
    <name>A0A5B0PLW8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLW8"/>
    <dbReference type="OrthoDB" id="2502883at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="255" mass="28316" checksum="2D92B6B213C959A2" modified="2019-11-13" version="1">MSRFGIDLFARENTPPQMGYHTDTENYRIHGIGPQLANLTMSALHPMFDNPGWNTVKPENVFPIVFKIGSLSLKDFQSLVATKANKLVDDAGGLINNTVETGSPRINWKVSLALKSAPEFKKALQYTVNEEKSYDHWIKTMKDLNADHTHASLHVHMVNPTSIAKKAKVAVDMKAHVLTQHAAQKAGSSSATGNQHGPPKATDFKAVNVIMDQIYALHPPNIKYLDRIPVFIHPTEHSQYIPLTGKNVQIWAHAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRP5</accession>
    <name>A0A5B0PRP5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRP5"/>
    <dbReference type="OrthoDB" id="10256463at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="291" mass="31879" checksum="92D53E638A1156B9" modified="2019-11-13" version="1">MANPYSSVTVFDAEESSKTVPADNLAFQDFSQNAPSDAGRPGQGTVRGNVYDPDAELQQRLAGSQSWFSIDAYSPYFDVETKTVLERCWRTMYPKDDYVEVVLAGQPDLYGPFWLPTTLIFILFFASSLSGALTSYLHSQSYDYDFSKLSMAVGLVYVYALALPACIWAAMRYWAGVEGRTIPEIINLYGYGLTVFIPVALLSIPPFPFLRSIMALAAFGISLGFLVRNLYPVLAAAPAKTGRILLIGVFGLHAIFTLILWFGYLSIGGNFEEVIVAPAPSEDVGKVPSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTP8</accession>
    <name>A0A5B0PTP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_017907</name>
        <name evidence="3" type="ORF">PGT21_019238</name>
        <name evidence="4" type="ORF">PGTUg99_025266</name>
        <name evidence="2" type="ORF">PGTUg99_034855</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="2 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000472">
        <property type="protein sequence ID" value="KAA1075563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTP8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1075563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1104276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="479" mass="56071" checksum="EC47D3AF8EAF5472" modified="2019-11-13" version="1">MQFWFFKYSPLIFHIIEKCSLALPPSTIGKKDLSFNLNELPQEGTADALIPSTFSIPSFSASGEDLNVKKECIEPSYKPYHSPTLHNPNNSIKREQGLLDSEKRNKKSRYGAEKKLAFPKEEDMIPNDESSSKSCARPNQAQVQNIKIEPQDPSSDISNLQENINNEPERNQKKDQILNVSDWNYVKIDPQHEILNVDGGLEPVVNLFEFLRKMKDEEDSGKVFCISRDKDVIFFLRYVSTPKKDRFAYPDFISESKRSSEILRILFEISERKLSLENYFCFFTDIRDKMILRFEPSLQSETRKVKAYKLNQVQSRIKYLKDITKISIILIILYLSLFGEHEADELKKQDLMNLVDFFEDFWKKCSVVEHHETNYEESVENLFHDLVNLKENESFHSSLRNSYFKYFTMPWKMVKYWIEKTGRGQIKNVVESGKYYKIIKSIINQIVFHSNYNNILNRINSGKFSSKGVALDELEPSFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PU17</accession>
    <name>A0A5B0PU17_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="14">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MUS81_1</name>
        <name evidence="18" type="synonym">MUS81_2</name>
        <name evidence="17" type="ORF">PGT21_008595</name>
        <name evidence="18" type="ORF">PGTUg99_020446</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="18 20">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the XPF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="14"/>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000143">
        <property type="protein sequence ID" value="KAA1128276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PU17"/>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21036">
        <property type="entry name" value="WH_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20074">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000307">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000003">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047417">
        <property type="entry name" value="WHD_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Restriction endonuclease-like"/>
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    </dbReference>
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            <begin position="759"/>
            <end position="866"/>
        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="144"/>
            <end position="158"/>
        </location>
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            <end position="182"/>
        </location>
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        </location>
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            <end position="374"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00023254"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1104048.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1128276.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1100" mass="120743" checksum="1A7AC6EBCC5D53D7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYB9</accession>
    <name>A0A5B0PYB9_PUCGR</name>
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        <recommendedName>
            <fullName evidence="3">UFSP1/2/DUB catalytic domain-containing protein</fullName>
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    </protein>
    <gene>
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    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        </source>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0PZY1_PUCGR</name>
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        <recommendedName>
            <fullName evidence="6">Mid2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10418751at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022976726" description="Mid2 domain-containing protein" evidence="3">
        <location>
            <begin position="34"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
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        <location>
            <begin position="39"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1106502.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="28435" checksum="A42FC1F9CCDCF38D" modified="2019-11-13" version="1">MWRKRRSPIMIGVVRSILLTSLVSMVHVVEVNGQAEPFDQRVPATTTTGYPSDSSPTNPSPRYPAPIINDPTAPNYGLNSSFPAGSTLNPTNIPGTASGVTSSPTTNSSAIPHPPLDPNSSSSSSKPPANQNTPPPLPGASSPNNINPNNAAASVDNHQQTQTIGGGLKPEILMAVVLAGCLGLVVLILGGLFLWSRCVRPRLRAASASNKAEIRNTDDDDDQKKKEEDNRVETADRAASQTSSSASRDVGSPSADRPKEDDLDQPVSTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5Q7</accession>
    <name>A0A5B0Q5Q7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5Q7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="11313" checksum="2ACE615FEAFCCA18" modified="2019-11-13" version="1">MQLLISSHSDFPFITFSTQLQLLDTSTPTLTLIQLLALLPIQHLLQTTKTHPKLPNMDYSLDGPAEDEHPIQVINTDENQPAKPLGVQDPDELDPTDKGMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QI86</accession>
    <name>A0A5B0QI86_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013056</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QI86"/>
    <dbReference type="OrthoDB" id="2511459at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="359" mass="41585" checksum="5168712B694E14F4" modified="2019-11-13" version="1">MDLLFGVSDMENLFAPRTNYRKRPFTPTSNLNRNTETTISSSTLSPPWMKNEKQGNLTIPVPPIFRTGIFKTLDMDEPNHQQSKQQPPTYQQPNNQNKPVFIEQPGLFYNGHHFTQFLRQYEMTADSLNASKYDQALQIGRFMKTEELKDQIELMDGYQECDWDTLRASMIELWGDKYTILYTTSDLVNLSKEFSRDNKTRQLRSRQDAVIPFISAFPQELQRNIKQHLNHNGQLPQAPDGSRLPPLWEHLTEAAGMEIKLEELAKEIESLKQQIQDIQSPVICNTPHSLSQLEPVTDHTELATDHTELATDYTELATDYMELATDYTELATDYTELATDYTELETDYTELVMDWNQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ11</accession>
    <name>A0A5B0QJ11_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ11"/>
    <dbReference type="OrthoDB" id="2499165at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="292" mass="33432" checksum="554A96A3BCFF38CF" modified="2019-11-13" version="1">MQLFLNPQFDDMEYFTHPKNRPRTERRPNRSPSKAMSPDEDYEDHSMSIDPFIHHPASKSTRRLKISRQHRISPPPPLPSPQLPYIRSEKYPAWLLGDAMAKSSANYLSQKTQEAPLTTPLSSQQQNTQTSPIRRSASSFLGTSPLKHHHFHTSLSRQSSSSNLNFNNKRPSLGRSISPDEALDRRREKFWELYEYDSTSPHLQARYFDKPLPTNEQWADLDIQNVAIIPDPQPGPPAVHLVEVFQPTRVDCTNPQPPSNSLSHIQLNSQQLKPHIPFGLSLLPSDYALHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QKX1</accession>
    <name>A0A5B0QKX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023851</name>
        <name evidence="2" type="ORF">PGTUg99_013241</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QKX1"/>
    <dbReference type="OrthoDB" id="107110at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="992" mass="112662" checksum="4E3BA1F311A80E23" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN21</accession>
    <name>A0A5B0QN21_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN21"/>
    <dbReference type="OrthoDB" id="10539628at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="265" mass="31015" checksum="B605EBB9F987C720" modified="2019-11-13" version="1">MYHFITNYRPTPWEKLSEEEKLLLATYHAHVHQEQDLDQQIQFRSFLRDIREKNQQLSRKRYLEKRVIRQERASPTPSVDTDNGDSDHTTPSQIAQNSEAPSQLQRDLVAAKELRARVENLQMTTLKRKETATASREEPPQNMEKSSAMKIDEIDSECSEATPKRPITPEIRILSKEDQIRLRESQKVLQKLIDNEEIESYVKGWNPWDEKKLHFPSPPKKNLMKFKRNEPGKKQSSSSTNFSDPAKWRKVTDLLQMASGLYQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNT4</accession>
    <name>A0A5B0QNT4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">4-hydroxybenzoate polyprenyltransferase, mitochondrial</fullName>
            <shortName evidence="10">4-HB polyprenyltransferase</shortName>
            <ecNumber evidence="10">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Para-hydroxybenzoate--polyprenyltransferase</fullName>
            <shortName evidence="10">PHB:PPT</shortName>
            <shortName evidence="10">PHB:polyprenyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">COQ2_2</name>
        <name evidence="11" type="ORF">PGT21_019007</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>an all-trans-polyprenyl diphosphate + 4-hydroxybenzoate = a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:44504"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:9564"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Mitochondrion inner membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
            <orientation evidence="2 10">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="10"/>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNT4"/>
    <dbReference type="OrthoDB" id="18170at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate polyprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1780:FF:000001">
        <property type="entry name" value="4-hydroxybenzoate octaprenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000003">
        <property type="entry name" value="4-hydroxybenzoate polyprenyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01474">
        <property type="entry name" value="ubiA_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1114703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="419" mass="45440" checksum="C00E140FEA9C26F5" modified="2019-11-13" version="1">MASEAWRKTCSIPFRTSSLVYRSTTKPNPAHLSKIPQHSNHTLGMLHRRLFQATVCSIGHQPSANKSASLFRSLVPHRDSLRSSRPLKGWILRYQSNSACDHDAPNAPSVKLKRIEKEPKLNNLIKRIEPYLALSRVRAPIGAALLFLPCGSSLALAASSAAVSPQFLIAQTCLFGLGAFVMRGAGCTINDLWDRSIDQKVSRTQLRPLASGALSPANAIGWLGLQLSIGLAILLQLNIYSILLGASSLSLVFIYPLMKRITYWPQFVLGLAFNWGALLGSSAILGQTDWKVSIPLYMGHICWTIVYDTIYAQQDKKSDVLAGVKSTALLFGARPVPILAGFSTAFVGFTALSGFMNGCGIPFYSLAVIAPAIHLAHLLANLQPDSPSACHSSFIASAITGSLVWVGCLLDYCTRFFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPB9</accession>
    <name>A0A5B0QPB9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030873</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPB9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="283" mass="32524" checksum="BF74CEDDC1B9228B" modified="2019-11-13" version="1">MNSLCATYFCWISLLTDKAFFTLSTPTEDKIKITYKVPDLNDLPPDEPLIAPIGSMPLSEFFGGPKEDCNLKREQTEPFNVSLMPEPIGHAIASSSKRKPIDNFDAEATKDAFFQVGEKTQNDFSNHKGFMSKKGRVIRDDSNSSPSGFLFKGKCPMENLNGILHVENQCNSVSRIPNEIKCEIANVNSQDFLQEDPQKIIVTKNQWSPQKSETESAFHKLLQHLRNSEEYPKTNHQRFKKKLDLSAEPLIRRIHKNLQQQIFVYPKMKRILLLNLWPHLKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPY7</accession>
    <name>A0A5B0QPY7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPY7"/>
    <dbReference type="OrthoDB" id="10390790at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="220" mass="24261" checksum="C2EE262CAEA02B16" modified="2019-11-13" version="1">MTLRNSCAAVESAELRVPRSDILGPGEQVGPVNQPVYIPPIPTATVLPTSSGLQSDLRCIDDRIQQILSCVLPAAKSSELIGQDSVKSAEPSALHSVLRRSGPSSCPYTHLRADFNSEQYRRHPQTTPASDSFRGLRSDLRCIDNRTRRICVVILPSAESIKVIGQDQSAARGVARREVVFEGQTHQPVPILIPINTDDSRRFPRPTPTSEFFQFCQLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQZ9</accession>
    <name>A0A5B0QQZ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQZ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="285" mass="30668" checksum="150B3C82784C9B6F" modified="2019-11-13" version="1">MDEDYSSDFPVPNASPAAPSSTEAVSMDLDLIVEAPAAPTILAPAPISSPRVVPAFRIRGAANAAPHSSPTPLVPVNQNGGGARARGRARNRGQARGRGLDPVRVRGRGRGFVPAPLCGRGHPSGSNRLPTGPVISNRAVVGRGATPPPAPRGSRLPTPQLSPGGPFDRHGSLTSADNSWVEPHVRTRTRVPSPAPFGFYDVDDYHRLAPHQVAALLDRRPDFGRYDQHQFAPQLYTAFYQDVASALVGHFTRHPAANTFEREGRVALFRFVGRRYRSALRRVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXX6</accession>
    <name>A0A5B0QXX6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXX6"/>
    <dbReference type="OrthoDB" id="2514918at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="158" mass="18480" checksum="6FBECB63FF31CEE0" modified="2019-11-13" version="1">MRITLRNFGHEFQSTKLVNAGHNDNEIRQSLQENHSIIVSQRTLTRRKEDWGLILHASQQIANTEEHIKKYFDQGLTYSQIHHALTTSHNYTHSKRTLQRKITAMQLSRRLDNLDTARVTIEAVVSCVMHLHLTPEGRNVGYRRMRQLLQTMFGITLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZN7</accession>
    <name>A0A5B0QZN7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZN7"/>
    <dbReference type="OrthoDB" id="10287936at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="170" mass="19019" checksum="C5C6A97B5650EDEF" modified="2019-11-13" version="1">MLPHSSSVWDRRIQGTKLYLCCYDLISKAPKWQDYNAVMSKKLTNVNPSSPLPSSLPNSTTAETVHLTSDASGDESVHGEPTRLMGRKKAKLAYQEEKLDASNHEHLKKMATAHIDIAEVAKKQQTSLAKAMHAQHSALERLPDEAVMNKDLTGANDDVKKYYMLQRKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1B0</accession>
    <name>A0A5B0R1B0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1B0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="238" mass="25715" checksum="2F067C80CEE96675" modified="2019-11-13" version="1">MIGISWGDPIRAAWELGIGIAQADPRFPQSCIVARPHYLGPGRSSFLTVYCYSNTPPHHPSSPTPSPTYSHHPITTPPPHRSSASSPRLESDDRPPPCRFTHPSSGRTRTRTEIARPPPLASGRTRNRSDTARPPPLVVPGPSFPAPALRFVAGYLIWRPPTLVASPFKRRAVLGLERTPPGLRHSSFPACPPISRCFAGQAAAAAAVVALGVVGSTWYPPPTTFICFSRCARQLVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R214</accession>
    <name>A0A5B0R214_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_011111</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R214"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="501"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="655"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="732"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1118954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="794" mass="87162" checksum="2B45F9407A7BA9C1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2E2</accession>
    <name>A0A5B0R2E2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2E2"/>
    <dbReference type="OrthoDB" id="2504795at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052717">
        <property type="entry name" value="Vacuolar_transposase_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169">
        <property type="entry name" value="DNA REPLICATION-RELATED ELEMENT FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169:SF29">
        <property type="entry name" value="DNA REPLICATION-RELATED ELEMENT FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF659" evidence="2">
        <location>
            <begin position="202"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="736" mass="84383" checksum="6E936E54B58D1CAE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2Q5</accession>
    <name>A0A5B0R2Q5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031659</name>
        <name evidence="2" type="ORF">PGTUg99_033126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2Q5"/>
    <dbReference type="OrthoDB" id="2496119at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="187" mass="21198" checksum="56FCBEFB68E51007" modified="2019-11-13" version="1">MATQAAEYPVSDSGRMSLESARKARCSAFAKERDLLGIAEGEGAIKDEQRDDNLQDASSATNRPKAQQPRPSLRSQTRHRSKPLEPYVSRPLEVESIIQRNEYRRARVELGLYLLLDQPPGEENSLVFPPSATCYDAEESHWPDSFSHLSDRSVPCNSSLSSYFTNSRPPSILQPVKEWWCSRWSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3L7</accession>
    <name>A0A5B0R3L7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3L7"/>
    <dbReference type="OrthoDB" id="2511691at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="349" mass="39072" checksum="2A306F77A4A16606" modified="2019-11-13" version="1">MPNLAILSQLKKSQTLPKTQDSSNHSNTSVKHSKMVSTRASQRRGSTRSQSSISTSNKNRKQRIITANKKQVEKSNNGVQSKPTNSNPDSDSDSNYDTRIGFTLENFGSHLPIDVQEALQLLQQNYLKSKLMLALIGNVGEKQIPNKPSRKQCGWNRYVAFSLESLKHPVPPKGTSEGWDQRNCEMGKALASLTADEKEVFSSRVFQHFSKIPCGYDKDDDDYVENEDSELEDDEGRGSLTPDEISLYMPLYHNLVNHDKVKAFIAKGPESKGHNAGQAYKQALKNIMNLNSELYTVSNVYNTTYYLLSATQTPGLNSFCKEYSNNAAWLALAQKELNSKETFEAYSHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3M6</accession>
    <name>A0A5B0R3M6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3M6"/>
    <dbReference type="OrthoDB" id="2510301at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="155" mass="17103" checksum="62CF9527EE185295" modified="2019-11-13" version="1">MARSSVTPHGTPSQLPSRQSTWIVTPTASSSYVRPSRNLQRSVGPTTQSQRQGKSLGSESEAESVVQIPPNQQSKKKRTRKPPSTKQRKASITTNTDDDSDKVDGVIDHTQDSDKENSKALGPSKNKTSYDNIREYFGAPFHAKEKDKGDKLSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R870</accession>
    <name>A0A5B0R870_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_020788</name>
        <name evidence="6" type="ORF">PGTUg99_019202</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000237">
        <property type="protein sequence ID" value="KAA1121702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R870"/>
    <dbReference type="OrthoDB" id="1654884at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000015">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.5, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1116627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1121702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="271" mass="29338" checksum="9E8FAEE4D0CAEE9B" modified="2019-11-13" version="1">MSLPSLFRQANRILTIASSNITLRPSSTRQYSSSSSRTPLTLDDVLPATPTSSSPSIREGGGDFSAVTEDYQQSLPPGRPQERFGTRGGLPHGINRRTAIELGADPLRRRSGEKSHYSLLVHSTPNNTRLTLTHTPVAYLPGSSPGHAGFKSAYPLAGAVVARVTAGSVGFKRGRRQEYEAATQACLAMFAKIRELIKLPTVARVNANPAVREGVPRELEVVFNGFGIGRDAFLASLLNSQATDLRELVRSLRDSTVVKIGGPRPKKRRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RAR1</accession>
    <name>A0A5B0RAR1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cellulase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_015029</name>
        <name evidence="15" type="ORF">PGTUg99_029292</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RAR1"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000124">
        <property type="entry name" value="Exported cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142:SF1">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474540" description="cellulase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="13">
        <location>
            <begin position="81"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1073543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1122133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="389" mass="42776" checksum="4DED63DB4DDB89F5" modified="2019-11-13" version="1">MAFNQYTFWQGLFMLLSAMSAISTTAPKSDSNPGMNENTVLPRLAGVSQKISFSQNTRGWDFGAQTSGQYTPGSTGVAPPKTQITHFLKQNANLLRVPVAWEFLQPERNTKLNATNVKVYREYIEEITSQGAYAIIDLHAYARYKSEIVGESPNMPASVLVNLWLQLGGLFKDNKKVLLGLSNEPHDQDIDKWAVTVQKVVTALRKDGIDNILLLSGTDYSSLKAFPEWYKSMKTVKNPDGSFEGLWFEVHRYLDTDNSGKSTECVASHADEVVNVAKMLKADGRQVLLGETGGGSTESCMTYLPELVQAVVEAYPVFSGFAIWSAGAFGATYELVVTVQDDASPTGWKDQGNWLSIKKFLPNKASLAHRNKPSTGGAKKNARRSIVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RIN4</accession>
    <name>A0A5B0RIN4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008205</name>
        <name evidence="2" type="ORF">PGTUg99_011288</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000181">
        <property type="protein sequence ID" value="KAA1125239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RIN4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1125239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="126" mass="14581" checksum="0024A28B9EE945EF" modified="2019-11-13" version="1">MRCQRWCDCTHHYSPDIGDRDAKRYIKADKGLLVLCRINPPPILYWNPFKAKLHLNRQSSHPASKCNFILRFSLAFSSSKESYPPEDQPAVEAPPSLAWVHASPVKFRGKRSLAVSRRIGYEMMMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJ48</accession>
    <name>A0A5B0RJ48_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_013970</name>
        <name evidence="10" type="ORF">PGTUg99_001850</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000182">
        <property type="protein sequence ID" value="KAA1125148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJ48"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF3">
        <property type="entry name" value="RING FINGER PROTEIN 141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474784" description="RING-type domain-containing protein" evidence="7">
        <location>
            <begin position="16"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="127"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1105634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1125148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="236" mass="26133" checksum="986F57E7975F2779" modified="2019-11-13" version="1">MDIVFLLLLAGVCFARELPDGSSLVEFREDNPYFFHSIDIKSAVIQRGSDGSTSCHVSAGNADLQGENSSLKEIVIQDGAGGRSPGKEVPTEISNSEDSPHYLKFDGGQVASHDLKLSGNAREAEMCSICLNSRPSTQDPVFYFGQECRHQFCNECIDNWLNQQRKQATCPVCRCPVEWTKELAAKLPAEELQFDQPCRSMMQIIRSLSREQVAILTALVLFFGILITIGQILPNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RR77</accession>
    <name>A0A5B0RR77_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="6">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_032395</name>
        <name evidence="11" type="ORF">PGTUg99_034669</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP-specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 8">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000169">
        <property type="protein sequence ID" value="KAA1127313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RR77"/>
    <dbReference type="OrthoDB" id="1664372at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000005">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="9">
        <location>
            <begin position="41"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="6 7">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="54"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1115147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1127313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="340" mass="35780" checksum="DEB752069752677C" modified="2019-11-13" version="1">MLRHPRVLSKAIKLTHYGQSRTFLTSTQLQSPYDLSIPNLRIHKDTKVICQGFTGKTATFHCKEGLAYGTKFVGGVSPKKAGQQHLGLPVFGSVTQAVKETKPDATVIYVPPPFAADAVIEAVENEIGLIVCITEGIPQQDEVRIAQVLKSQSKSRLVGPNCPGIMSPDVGGGCKIGIMPSNIFSPGCIGVVSRSGTLTYEAVDQTTKVGLGQTLCVGIGGDPFPGTQHIDVLKAFMEDDATKGIVLIGEIGGSMEEEAAEYLKQFNKSRSVPKPVVSFIAGRTAPPGRRMGHAGAIISGGKGKAEDKVKALEEAGAVVSDSPAKLGDLMLKAMKQSGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S461</accession>
    <name>A0A5B0S461_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011531</name>
        <name evidence="3" type="ORF">PGTUg99_037454</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S461"/>
    <dbReference type="OrthoDB" id="10520558at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="173" mass="18041" checksum="3D45E6DDC6DA388C" modified="2019-11-13" version="1">MERQLATPRRPGLAGRAAEAEVGLPLGGLRLLHCGYAVSPFALAGAAGTFLEGARPQASSSSYRILAKQLAHHPNTKKMSSDTTPNPLGSQNAGTEEIVGTTTQAAEGGRPQKGTDIQAAGRTIATSKKSRLSTVAERQQSTTGPGDEESNPEEENNADEVLGLLRAAPTKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S4V4</accession>
    <name>A0A5B0S4V4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011643</name>
        <name evidence="3" type="ORF">PGTUg99_037421</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S4V4"/>
    <dbReference type="OrthoDB" id="2505972at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="586" mass="66352" checksum="3F588D7792339F9B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S9J4</accession>
    <name>A0A5B0S9J4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027341</name>
        <name evidence="4" type="ORF">PGTUg99_001031</name>
        <name evidence="2" type="ORF">PGTUg99_009613</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000519">
        <property type="protein sequence ID" value="KAA1063885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000059">
        <property type="protein sequence ID" value="KAA1134462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S9J4"/>
    <dbReference type="OrthoDB" id="2500295at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1063885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1134462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="113" mass="12967" checksum="FF4DA5A679A6865F" modified="2019-11-13" version="1">MLLLGSNYRLLPTPLEPSVAQPIAVQSIIQRNLYRHAKVELGIYLLLDSEPAESNDNLSSLSSTGHQSSSSTEDYWNDCSETSISFDEFIPAHLYTPRRLPITQLIKNWWRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SKH0</accession>
    <name>A0A5B0SKH0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018801</name>
        <name evidence="4" type="ORF">PGTUg99_017377</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000002">
        <property type="protein sequence ID" value="KAA1138481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SKH0"/>
    <dbReference type="OrthoDB" id="2506656at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138396" evidence="2">
        <location>
            <begin position="24"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1138481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="126" mass="12139" checksum="C807E09284F5DED4" modified="2019-11-13" version="1">MQFLTGYICIIATLMNISLQVVAQAPAGPGAVDAETSDVMAALGGGKPGAGKSTGKGAGGSNAMQTNLVSAIDGFLREPVPQGASGNVFQLVIQYLEKAGGGAGGAAGGSAGKSGRSGVSRGGKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SML3</accession>
    <name>A0A5B0SML3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dihydrolipoyllysine-residue succinyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">KGD2_2</name>
        <name evidence="13" type="ORF">PGT21_010472</name>
        <name evidence="14" type="ORF">PGTUg99_034131</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000002">
        <property type="protein sequence ID" value="KAA1138669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SML3"/>
    <dbReference type="OrthoDB" id="5391403at2759"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000007">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004309">
        <property type="entry name" value="PRK05704.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="12">
        <location>
            <begin position="86"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="159"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1108379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1138669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="518" mass="57082" checksum="58F321DBC262876D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJM8</accession>
    <name>A0A5B0LJM8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_003335</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJM8"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45977:SF4">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45977">
        <property type="entry name" value="TARGET OF ERK KINASE MPK-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="191"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1064449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="229" mass="25576" checksum="FA25F65760A244CE" modified="2019-11-13" version="1">MDHHPTPSDSESAADQPGPSPGGVRPTEEPVEAQPQPAEMTADIDDALLQPPPALLETEQEVIARQEAWVQMMAPLTPEQRLDIQLYADLTTRVWEQLADNPEAPPEVLPPADDLLEIAENFQRTLQSLPQRTIERIDEFFARAAQQLGLLGQQRENIHMLRQNVAPFLDGLDITTLTSLQANVAQPDSPKCTICLQHYADNDVIVVLPCHPDHHFHWSCIQSPNFLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPF1</accession>
    <name>A0A5B0LPF1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020863</name>
        <name evidence="3" type="ORF">PGTUg99_002619</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000110">
        <property type="protein sequence ID" value="KAA1130274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPF1"/>
    <dbReference type="OrthoDB" id="2502295at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="799"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="889"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="800"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="898"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="988" mass="111831" checksum="716B0EA84424D382" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPS2</accession>
    <name>A0A5B0LPS2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPS2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="126" mass="13864" checksum="A39BB3CD85614059" modified="2019-11-13" version="1">MDTDWRYETIPMSLGGCTKSLFSVFEGLRSQSLEQSKTIPTPTWLLRGCQPTSDKAIDHQVGGLHANRDPPALTCFRERVALEAGLSRGIAFIKISQKVTSGTQPSSPHHRPTLDRPGAPRYPFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LR84</accession>
    <name>A0A5B0LR84_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019677</name>
        <name evidence="3" type="ORF">PGTUg99_012048</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000110">
        <property type="protein sequence ID" value="KAA1130298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LR84"/>
    <dbReference type="OrthoDB" id="2515431at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="373" mass="44069" checksum="14CB28B8064FF586" modified="2019-11-13" version="1">MSDSSPYPTLTYDIISTTIKRESELTYSYYWKVKEIRTYQVTTQKTYLRSNNELLGQNQHKRVIAILIKKTPQNIAAEVGIIRQPRPSPTPSESPRRREDTSNPTPDPDRRGNTPTTTGRRDSVNSRESTGLEIITETRANNPTNEEVFRRLPIEERFERYYYRMAENRAWLDRVSNRSTEEAIRAHLNYCREDFSEMIALRGRDNALIRTRGWDPSSIPASHYLNPIVNPQPRDNTQQELPAARTIDNNRNTHENLNQANPPNPHLRNRTQRFEEENINRQINPQLERRHTMRDPPRPRQDHEDNRERFQGPICTRAPRRNREWRTSIYSEMVRIGRSLQGTYQHLEEGRIRTQRGNQNAPPENHRDNQQGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRW8</accession>
    <name>A0A5B0LRW8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_012273</name>
        <name evidence="4" type="ORF">PGTUg99_024993</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRW8"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045899">
        <property type="entry name" value="ATL71-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46719:SF7">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL71-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46719">
        <property type="entry name" value="TRANSCRIPTION FACTOR C2H2 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033845869" description="RING-type domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="82"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1067182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1071585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="148" mass="17521" checksum="233A37C2EFB0C10B" modified="2019-11-13" version="1">MVRFLLLIVLLQAIRLISGLQIEPEDNQGRFKQLYNSCHQKMISSFQSMSVGLEELVEYSEYLLNVSDPKWKVDSVTDGIDCSICLERCKVYNKIKKCPNCSRPFHKRCIEKWEAKKPTCPVCRTITEQRLELKPYRKRMGFFRCYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LT69</accession>
    <name>A0A5B0LT69_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar-sorting protein SNF7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">DID2_1</name>
        <name evidence="3" type="synonym">DID2_2</name>
        <name evidence="2" type="ORF">PGT21_000436</name>
        <name evidence="3" type="ORF">PGT21_000826</name>
        <name evidence="5" type="ORF">PGTUg99_000372</name>
        <name evidence="4" type="ORF">PGTUg99_024681</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000185">
        <property type="protein sequence ID" value="KAA1067073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000099">
        <property type="protein sequence ID" value="KAA1088570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1132933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LT69"/>
    <dbReference type="OrthoDB" id="10266568at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1088570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1109591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1132933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="205" mass="22430" checksum="20E987249C28532E" modified="2019-11-13" version="1">MSGLEKSLFQLKFTAKQLQKQSRKAAKDEVTEKAKLKKALQQDNVDGARIYAANAIRKHNESLNLLRLSSRIDAVSSRVETAVTMRSVTSSMTGVVRGMDRAMESMNLDRIAMVMDKFESQFEDMDAQAGCMESAISQTTASSMPQDQVDLLMQKVADEAGLEIKHGLGEVPKSSVGVAEQEPVANQEAPEDALAQRLKALRPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LU11</accession>
    <name>A0A5B0LU11_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SNF1-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SIP5_1</name>
        <name evidence="4" type="ORF">PGT21_009661</name>
        <name evidence="3" type="ORF">PGTUg99_030775</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LU11"/>
    <dbReference type="OrthoDB" id="21471at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24139">
        <property type="entry name" value="SIP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039301">
        <property type="entry name" value="Sip5/DA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315:SF1">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="449"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="527"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="465"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="551"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="596"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="646"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="681"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1071491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="767" mass="81834" checksum="4450692DD26D01DF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LW18</accession>
    <name>A0A5B0LW18_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LW18"/>
    <dbReference type="OrthoDB" id="10299721at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="494" mass="54351" checksum="9ACEEBAB01798166" modified="2019-11-13" version="1">MSSMSDYVPTETESERETREETRSPEPRLNHQNPRQTQAPPSRTGPIRNTTHRNGRLPGQSYPPLRGSVAAAAAFARQAGRPTAAPTQPTRSGRAALIPSVGAAERLARRSVPPLGDVADPGEVRVDASTGFPNGIIDLTHLDEPVNEEYVQELEGLFELRRGYAAVGESLAYVAPPRQHATHLYSQLAILQAIERYHNAVLAVPRAAALPEVTPANQAQQFQYAPVFKDFVKRKARELLMTKNLKVYGSDLPRGAPATLKSLLVLVLVGQVLRIITQFRLASMITEQLTVHFQDHINAQPTSFKREYLPRGYADGDPGAVASVDSLVRNKLRKSRGMMRDLLLTNIQAPAGREITLPIPTASALMVNMRTSMTPPIPNSTAVVAPDPQEARGNQTHLKARLAYLRILTVMQLVGRGPRDAGRQWRNIDEHLRHLEAMGRDYRTVLDRALFNGDQFFLGIDTSTIKLPTNQEVEDLMAAAGEGVDHEDATLAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWR4</accession>
    <name>A0A5B0LWR4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWR4"/>
    <dbReference type="OrthoDB" id="10305455at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="120" mass="12592" checksum="307460190A49A509" modified="2019-11-13" version="1">MALRLALESLSQSGRYFLQGSRDLPLANDSDDGSLHSKGAMKRPSPLAWVFAAKPSGGLLSVLAGLKAQQARCPPANGISRPSSSTSFLSELLSPTCPPRGSSQTSPNPRIAGWMPMIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZI7</accession>
    <name>A0A5B0LZI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZI7"/>
    <dbReference type="OrthoDB" id="2514325at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023099229" evidence="2">
        <location>
            <begin position="18"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="319" mass="33657" checksum="7426ECA30A7030BC" modified="2019-11-13" version="1">MLSAVYVACLLAASAIASPVQHNPRYVAGSYSQDSAHSNEDHARGASSTPFSSSSYSSDRSNSDASHKSGSYYNGGSPYLGGGAAGFGGYGNYLNGGYGAGGYNGAASAYNSASAYNSASAYNTASGYNGIGSNGYGIGNHDPSMYAGGASNFGSANSFSSSQSQQSYSMSTVNSAFSSLNSYLENMNSRLISGQITQQAIYQVVQQITQYFESAMSQATYCGECVNGQSQFSSVASSSFSQFTHFMNQVERQFGGSIFDQFSNPMSRLSTSMQSFFHRASSSSFSMTSQSFIPSDFTPLAQRLMPDIVPSLSQFQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZU1</accession>
    <name>A0A5B0LZU1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015951</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZU1"/>
    <dbReference type="OrthoDB" id="2496113at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022894556" evidence="2">
        <location>
            <begin position="20"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="757" mass="85115" checksum="B912B5F5903FCDBB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7E5</accession>
    <name>A0A5B0M7E5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7E5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="11300" checksum="F85C87513E432501" modified="2019-11-13" version="1">MRPCIGIASKKRCIALKKRCIASKKRCIASASYRKAMQTDATKLWCIASHRFADAILIHCIEESNASDVMRGPASLLRCNAIKARYDAIPMHASHQFHRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7E8</accession>
    <name>A0A5B0M7E8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027895</name>
        <name evidence="2" type="ORF">PGTUg99_016624</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7E8"/>
    <dbReference type="OrthoDB" id="10392459at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1135218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="179" mass="19788" checksum="AAC2833E5FBA2428" modified="2019-11-13" version="1">MPVPSQPITTVLCPETQEAASTALLHPPCKTSVLVECLLFIQDVRPTISPRKGAAPPLDLRWTKITAKPPLTPWVTDIQSMTWDEFQTEAFKFLGSQCAHLIPAFEAANKDKKIAWYASISGHPKYDSEKKHVILGPIGYLNFVNAAYSAYPAAVLFKLLMKDPCEEDDGPCNVSVLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7F4</accession>
    <name>A0A5B0M7F4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine-protein kinase KIN2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">KIN1_2</name>
        <name evidence="2" type="ORF">PGT21_019252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7F4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="237" mass="25185" checksum="2A4A14A09BD34C86" modified="2019-11-13" version="1">MVADNPPRISAIRWRIPASASGYPPAGAVSAADGCFSAQTWRISGYPKGYPVPREATPPGGIPSRREGFLPTRGTGWSAWLSRPTFLPAPRILNVLRQVLLDGLARYWDALHRKRLSPPFSPGGLQEQQQTAAQAQPDHVSDDIPTDAIPMSAPISGQSGPMAAFGAKSDLDANLSSRSRHHQRGSSAGGEAGHNDVPASEGEGKVVLKSSASGPNWRPRMSSTSWCTHPLVGRNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8H5</accession>
    <name>A0A5B0M8H5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8H5"/>
    <dbReference type="OrthoDB" id="10556228at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="429" mass="47724" checksum="ACBD5477BE897D46" modified="2019-11-13" version="1">MPRRCKPVQPSPDSRRTITQPLLSERTSIGTSSQPKKRTNAPSESDSTPHATPVQSQQTRKKVPKKPKKTGPAAANSESNTTAKDVDLAQDSDEENSKVKKRQRKNPEEDDVKVFFSEPFRRKDDPADKPASTYSCLWCKKEVRVSASSLSNLRVHRDGSRQSGRVSDGCPNCAKAIAAGAKLPPTSLEEEKKKKKNGNGDLTTHFARVEKFDNTILNQIVVLWLLRQSIPWNRVEDPYLKAAFNYCEPAAILFKRKWAATGARKAYLELQEAMLYRLKELGLESPPPPKLKKAFLGSFPYSNKMPKITKEDEDGNEGDDEDDDSNNGRSDCTEGEKENSDDDDNDDDEDNDDDNDDSTEDKGENGKKDGKKNGKKDGKKDGKKDGEKDGKKDGKKDGNKSSSKANRNDSNDLNELTTANSSEEQPEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MA22</accession>
    <name>A0A5B0MA22_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025682</name>
        <name evidence="3" type="ORF">PGTUg99_020212</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MA22"/>
    <dbReference type="OrthoDB" id="2505192at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="595" mass="64775" checksum="2C5187C5584E74C6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MF29</accession>
    <name>A0A5B0MF29_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MF29"/>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051279">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
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            <begin position="13"/>
            <end position="22"/>
        </location>
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            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="420" mass="47006" checksum="D696259C3AC56D41" modified="2019-11-13" version="1">MVSTRASQRRGSTRSQSSISTSNINQKQRIITANKKQGKKSVISNKKKKTSRNKQTGDTSHSPSHPSTRTQTQVEKSKPTNSNPDSDSDSSDSESSDSDSNSDTRIGFTLENFGSHLPSWSVNELRRTLQKKKNPSNRLPVDFQEALQLLQQNYLKSKLMLALIGNVGEKTVNKFLGENKPSRKQCGWNRYVAFSLESLKHPVPPKGTSEGWDQRNREMGKAWASLTADEKEVFSSRVFQHFSKIPCGYDKDDDDYVEDEDSELEGDEGRGLLTPDEISLYMPLYNNLVNHDKVKAFIAKGPESEGHNAGQAYKQALKNIMNLNSELYTVSNAYNTTYYLLSATRTPGLNSFCKEYSNDAAWLALAQKEWNSKETFEAYSHGRQIQANLEAITNGPEGESGRDADQLKKNLLNALNDLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0MFS0_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">PGT21_024474</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1074906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFS0"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="56" mass="5806" checksum="C200FC399267EACB" modified="2019-11-13" version="1">MSNRNNPNEASPTAIPIPSQAQAGPRSQKPQAKANPASRVPEADRSIPVVQSASEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGD9</accession>
    <name>A0A5B0MGD9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000147">
        <property type="protein sequence ID" value="KAA1075761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGD9"/>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="172" mass="18414" checksum="47AF4C54FE7AA7DA" modified="2019-11-13" version="1">MIDSFITADRLSSQAGSGPIAGYSLSPIVFLVTDLIATLRIKRFYGLGGVQPVGVAFRVDFISSNSSNHATAILLKSNLTALGLALSPVPDDNNPSPSVCQQFFLDQLVYNDKASNPYSDAHYTATTIGCMTDSLIHLADKAGREKLVEIFGTKVLSTGTNGDLERICDKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGU5</accession>
    <name>A0A5B0MGU5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA replication regulator SLD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_035052</name>
        <name evidence="9" type="ORF">PGTUg99_036386</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Has a role in the initiation of DNA replication. Required at S-phase checkpoint.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGU5"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22289">
        <property type="entry name" value="RecQL4_SLD2_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1460:FF:000001">
        <property type="entry name" value="DNA replication regulator Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021110">
        <property type="entry name" value="DNA_rep_checkpnt_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040203">
        <property type="entry name" value="Sld2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124:SF1">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11719">
        <property type="entry name" value="Drc1-Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="49"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="301"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="462"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="642"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="711"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="333"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="351"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="538"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="642"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="711"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1076145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1091515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="752" mass="82159" checksum="CAEF2975AAA94D1A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHZ6</accession>
    <name>A0A5B0MHZ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine-zipper-like transcriptional regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LZTR1_11</name>
        <name evidence="4" type="synonym">LZTR1_25</name>
        <name evidence="4" type="ORF">PGT21_026830</name>
        <name evidence="3" type="ORF">PGTUg99_011027</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHZ6"/>
    <dbReference type="OrthoDB" id="2496157at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="157"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1091143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="423" mass="47687" checksum="43EE68901A868358" modified="2019-11-13" version="1">MHNRNEPSQPQPDPPVISNHNSSIVGRETRTSSKKRVRPGKDIKRWALNKKKAAAPFEGSPSINQTEAAENYDSPDSELTNNDNNNNSEHSQQNQEQHVYFYFVPPHRQVDPNPSEAITTQNTLKQYHHLTHGTCVAAPRGGPAFCKVKYVPFATMSPEQIQGWEKLVCHFFDRTNYVEAVKNNGPQMAGDMWADGWRKCSKKESFGRYCSVGKLVKMMLRANYNPQDKATSIKEASDFIAIQLQHLAPGVFESYRKTLISNNLPSMAHMEYPLPYDALDFASFLTFTMYNFHNGPHKDSDANNWTLVCWIPIFNPRTASDDDPILADEGFDMLGGQFTFRKFQLCLDLHHVLGVTLCVFKSNSHTHQTLPGASPSDRYTRIVFSCQMSEAMTHAVVAYINGTAPTLEVAGQQKQISNAQKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJR7</accession>
    <name>A0A5B0MJR7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008576</name>
        <name evidence="2" type="ORF">PGTUg99_003068</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJR7"/>
    <dbReference type="OrthoDB" id="10313965at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1135487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="218" mass="24336" checksum="6AEFF3A4026F754D" modified="2019-11-13" version="1">MQVKCEVITKSSENKYLFISNSSSNQVLDLTFSGACIFNQKVKSWDFDKGYYNIFKVLLSSDAGLHDAHSRVYFQENKIVNTIVAPCLDSYGYNLTIGVMYQICGTITRDDTCGTAWEFNPLLVDSPDSRRPETTGDGFWVKGSGQVRLVSKVSIQAGAQGSYHQILLNHQPRIQIILPTSSLYIRLDTEELVGLTLEFCGLFIGEEIESGVIKLLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKZ3</accession>
    <name>A0A5B0MKZ3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKZ3"/>
    <dbReference type="OrthoDB" id="2506088at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="207" mass="23837" checksum="C90EE9B4072D3FBB" modified="2019-11-13" version="1">MERVPDILLRRFSHHVIKQIHQLKLFEHDVLKKEYFVLVKMKSSGDSPQEVERVESIWSVSRENQTRYFIKGRRFSQGAIHPFYQMRVIENVNHVDYFEASDIVACLNAQHNCQSGRCPVVQGPRNKGQKHEGTKTTYKIHHNDNQSFILNSASLRDPVSHRKLASINIPHASDWATAIETGRARWQSSARQQTPQTRASSIAPSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLA2</accession>
    <name>A0A5B0MLA2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="3 9">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HAT1_2</name>
        <name evidence="14" type="ORF">PGT21_006877</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic component of the histone acetylase B (HAT-B) complex. Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. Involved in DNA double-strand break repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the HAT-B complex composed of at least HAT1 and HAT2. The HAT-B complex binds to histone H4 tail.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 9"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLA2"/>
    <dbReference type="OrthoDB" id="10253098at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013523">
        <property type="entry name" value="Hist_AcTrfase_HAT1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21184">
        <property type="entry name" value="HAT1_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038084">
        <property type="entry name" value="HAT-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="13">
        <location>
            <begin position="9"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="11">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="11">
        <location>
            <begin position="241"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="264"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with histone H4 N-terminus" evidence="12">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1077412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="424" mass="49998" checksum="6C857CA6F2514369" modified="2019-11-13" version="1">MAGEDPERWACDGTGALSLRLARGKVAADQLAPSEQHLISSFHPKFVYPLFGDEETIYGYEDLDIQLAFNSSTLKNYLAINYAAALPEPDPVEKIIYKFIPPDYTKSEATFNADLESSGTKKFIPPGKKISSYRTLPSKGKTKSEHFLTSKRWERCTDDQMEDEQDVIYELWAANWKSPGFREYHRRMQILVLFYIEGGSYIEEDDDRWEFVVLFERRKVKTADGDSSPYSYHFCGYVSLYSFYHYPSSIRLRLSQFIVLPPYQSNGHGSMLYSQIFQYLLTRPEVAELTLEDPSESFEDLRDREDLKLLFDNKVFDEKPLSELVPVPSEWYEKTRTKWKLANRQFARLLEMALRWKFTTLEESDNTKLERLYRIAVKERLYRFNYDSLVDLSKEERREKLQDTFQNVMDDYDRLLRGERRQID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP29</accession>
    <name>A0A5B0MP29_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000218</name>
        <name evidence="3" type="ORF">PGT21_025892</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000138">
        <property type="protein sequence ID" value="KAA1078705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MP29"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137266" evidence="1">
        <location>
            <begin position="19"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="359" mass="41288" checksum="1C19835F72B832CD" modified="2019-11-13" version="1">MLTLRSMVLTTLITKAWAAMFLCDIEPAKSPGHISWNYMILDSEAPQGTGTFILKKSDKDGGEIISDRPQGIEFKNQLKTKLWVYNGNKEGKYVEFPPNGVAQLESIDKNRLWVGTIERPYMMHISTDRYNKMASALLNAINEDRFILQEPHIPKHQEAMAHLNLKEGTNARPHIERSWEYPPKENPSDARPITLIRDWDKKLLKEVRYSGKSWWIIHRDFISDSENSGGLPFRITSGRPLFSKKNPYRVVKNVGKPKPIAVHVGDFSSLLPNIGAGKESSIFFNKPGDWAVITFFDQAAPLQHDVYKLLTSWNQDQVEEGIPLRWRERIIHSCAKIFKQEGSDYEFLRKVKANRSLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP41</accession>
    <name>A0A5B0MP41_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MP41"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="128" mass="14069" checksum="209CE47A502117EC" modified="2019-11-13" version="1">MRPHGCAHAGKLEDPLGIVHFFTLEGGVRLPGPHVSSASPQGGLVGFNVDGTYMPIGWRNRSQRSSSRSSDMARKLGAKRVFQRWVNDKECLSETRGLCSRQACPPGIDNKIRLNEILPTESRLKQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPH4</accession>
    <name>A0A5B0MPH4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024017</name>
        <name evidence="2" type="ORF">PGTUg99_010305</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000337">
        <property type="protein sequence ID" value="KAA1102535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPH4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="59" mass="6856" checksum="E2766CF6B6F08922" modified="2019-11-13" version="1">MNTEDLTEDSTFYKIRQRKHRLIRSSNERTYAVIQATNLLLDEKDEPVHGGSRPGKQPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MS78</accession>
    <name>A0A5B0MS78_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025791</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTI2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MS78"/>
    <dbReference type="OrthoDB" id="6417021at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110078">
        <property type="term" value="C:TTT Hsp90 cochaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018870">
        <property type="entry name" value="Tti2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32226">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32226:SF2">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10521">
        <property type="entry name" value="Tti2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="499"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="608" mass="69975" checksum="97A0E4EAAB34D9D9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUF3</accession>
    <name>A0A5B0MUF3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUF3"/>
    <dbReference type="OrthoDB" id="2507030at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="8718" checksum="3868A68C2CD020C2" modified="2019-11-13" version="1">MEDPDDRNMEQSWDLVITHCKSMWEKLINQDSIIMELEDHEEYEEEEMLQEEELQTQVENDEDILAGEDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWJ3</accession>
    <name>A0A5B0MWJ3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWJ3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023044338" evidence="2">
        <location>
            <begin position="23"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="279" mass="31717" checksum="1DD85E3D6AF57685" modified="2019-11-13" version="1">MKDKFRFVLLLWLEVQFRLVSLGPPPKKSSVIYPEAWIDTRFSGDTTSGVLASGNSLTVLRILCRSLDGFYTAPLDPGLSLTNYDAGRLESTRPQPIEIDFLTSWGKRVPEEMPEGSGSKKRPLSEDSDQLPEIEEQSHHDPNRDKRLKVDPTQSTTNLDLFQRYHRELLNLLNRVPPPLGLRFRSEKYSAMPGESIAKNDLLPSLHVEDPNTHLIRKPHQEPFDILNRVPPSVSLPSRLRIQKFRNTVKNPTVKVQGHRKVVSATIAIGKNQFQSQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MX20</accession>
    <name>A0A5B0MX20_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_029618</name>
        <name evidence="6" type="ORF">PGTUg99_028896</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MX20"/>
    <dbReference type="OrthoDB" id="2496683at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF89">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="54"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="713"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="837"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="727"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1081112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1131273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="971" mass="108274" checksum="AF56CF3734305736" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYP3</accession>
    <name>A0A5B0MYP3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029312</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYP3"/>
    <dbReference type="OrthoDB" id="332863at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070290">
        <property type="term" value="F:N-acylphosphatidylethanolamine-specific phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032:SF27">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="245"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="589" mass="65173" checksum="E21F8D128132DD61" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZQ7</accession>
    <name>A0A5B0MZQ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-1,4-glucan branching enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GLC3_7</name>
        <name evidence="3" type="ORF">PGT21_000525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000126">
        <property type="protein sequence ID" value="KAA1081756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZQ7"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000080">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-amylase/branching enzyme C-terminal all beta" evidence="2">
        <location>
            <begin position="18"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="114" mass="13549" checksum="71BC98599CAFEEE9" modified="2019-11-13" version="1">MNNLESQHKWLSSSHTFVSLKHESDRVVAFERGKLLFIFNFHPTQSYTDYRIGVEWEGKYQVVLSSDEKQRFGGHDRVDLQSEYFTTKMEWNNRKNYVQVYLPSRMVLVLGLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5J8</accession>
    <name>A0A5B0N5J8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_006294</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5J8"/>
    <dbReference type="OrthoDB" id="653904at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017054">
        <property type="term" value="C:negative cofactor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001046">
        <property type="term" value="F:core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22906">
        <property type="entry name" value="HFD_DRAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF5">
        <property type="entry name" value="DR1-ASSOCIATED COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="9"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="102"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="158"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1083763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="207" mass="22748" checksum="338ABA787554637A" modified="2019-11-13" version="1">MKKKGGSSRFPVARIKKIMQADEDVGKVAQATPLLVCMRPNSKQSPPTPPPLPSFHSFNLTSIFTPAKPTLILIALESPHKSDITFTDACSCFFSYAVSHEPIAKAVEMFMQSLVEAAVNEAQHRGSRKVQAYHLKQAIQVTPAFDFLKDIVAKIPDPVHGAAEDPEPSTDQRKRSTGKQKEDDEDAMGEEEEDSGLGGAEDDEEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6A4</accession>
    <name>A0A5B0N6A4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="14">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PMR1_1</name>
        <name evidence="17" type="ORF">PGT21_020614</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="14"/>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6A4"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000008">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000095">
        <property type="entry name" value="Calcium-transporting ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000028">
        <property type="entry name" value="Calcium-transporting P-type ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006413">
        <property type="entry name" value="P-type_ATPase_IIA_PMR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01522">
        <property type="entry name" value="ATPase-IIA2_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 14" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="800"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="873"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="986"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="105"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="541"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="541"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1084196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1059" mass="115806" checksum="38BD01B4DCEFA2D9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6R0</accession>
    <name>A0A5B0N6R0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000114">
        <property type="protein sequence ID" value="KAA1084941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="9"/>
            <end position="23"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="223" mass="25464" checksum="8FDD9D06C5F54AFE" modified="2019-11-13" version="1">MLLHRKILAKQDHTKRGLSKRDYPSPVEEDHISEDEILQSIKQRKFQPDILSDNKDLLKSALKTSTPSRPTPRFDLKNFQKSEKKRIGSSSMVEVEQEVPDGLYRSPSVSEKLLDRPKKETDGSPSKRSDLRNETAKPWERDVSSGMNQLSEMVVAGIKNKQVKIQGAAATNQLGVLNSTDNLVKEFDGQSEAITDRITQRARHTQNEYKQHHNDIKTTFREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N704</accession>
    <name>A0A5B0N704_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chitin synthase, class 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CHS3_2</name>
        <name evidence="2" type="ORF">PGT21_001102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000108">
        <property type="protein sequence ID" value="KAA1085055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N704"/>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="323" mass="35197" checksum="44EDAFF8FD7D4D86" modified="2019-11-13" version="1">MSTPHNHPHNRPQRQQPPLSSPFVSQTDLRPPRTAPLIHQNPAYNRYPDDRESNLEKDEGSYSPPHLDPSEPILHPTSRDNLKSGVRFGVAQSSYDSHPHLAPPQHGNPVMHRKKSLVRPDRAPIDPTHRLYNYRNHAAAMEADERGRVEVSHTGATAFDGSAALAQQPFDPHAHPLEEHSPNSNSPGPMNTTHSRAGLMAALGRPAPPSGYAGLVGEAGGARTAATGGLRRGQSILARRPGEAEESGLAILKRGETLRRTGRKRKSGVDAVETNSTPKPPISPWAIYCRAVTCCFPAFLLSTCGIKSADRLSAWREKMGILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NB28</accession>
    <name>A0A5B0NB28_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005189</name>
        <name evidence="2" type="ORF">PGTUg99_000380</name>
        <name evidence="5" type="ORF">PGTUg99_002699</name>
        <name evidence="4" type="ORF">PGTUg99_011607</name>
    </gene>
    <organism evidence="2 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000410">
        <property type="protein sequence ID" value="KAA1086401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000384">
        <property type="protein sequence ID" value="KAA1091712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000091">
        <property type="protein sequence ID" value="KAA1132196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NB28"/>
    <dbReference type="OrthoDB" id="10268158at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137425" evidence="1">
        <location>
            <begin position="21"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1091712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1132196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="114" mass="12886" checksum="55CAEB7B74C77B5C" modified="2019-11-13" version="1">MARIWVFWGGLWLVCGLILASPNMGRQAGEMEVECARCKDTAFSSFNPGACNEPLVCSQHRVATGGRCTFWEEERFRIRCTHCAYTTYEHPNLPLHQHSVRCTRSCPSHGTPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBT8</accession>
    <name>A0A5B0NBT8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_012972</name>
        <name evidence="3" type="ORF">PGT21_035821</name>
        <name evidence="2" type="ORF">PGTUg99_010293</name>
        <name evidence="5" type="ORF">PGTUg99_019614</name>
    </gene>
    <organism evidence="2 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000413">
        <property type="protein sequence ID" value="KAA1086142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBT8"/>
    <dbReference type="OMA" id="HQCNATD"/>
    <dbReference type="OrthoDB" id="2500653at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1095127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1121393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="159" mass="17643" checksum="8D0891F6C20A53DF" modified="2019-11-13" version="1">MSESSSEHSSTSSILSDSPQPSSIRDHSDQGQTSPVSEISELSCKEVQALKEYSHSVQNFTYQLFEKFRLELEAKGRSSKSVTENDHPSNPSMKPNSHSKTTANVGFHSTDLMIENSPLSPSFNPANAQSNPATRRRKPSDYPRRKKRPGPDFPDHLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NCB8</accession>
    <name>A0A5B0NCB8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012746</name>
        <name evidence="4" type="ORF">PGTUg99_022006</name>
        <name evidence="2" type="ORF">PGTUg99_027666</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000506">
        <property type="protein sequence ID" value="KAA1068216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NCB8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137442" evidence="1">
        <location>
            <begin position="21"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1136019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="148" mass="16929" checksum="81B0D472381561DC" modified="2019-11-13" version="1">MRLPLALAYILFTAFPIVKSITSRPEIRHGAKAWSRNGAEPFYQYDSQPTTHTVGENSYITDCLVRNTGRVEVNNPFPVLCHAQVIQYPTKRGPPSVGNMITLEHQNTNVEIQLTYLKPEDGSTENHFVVIYHFNPDDYKLLERLRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND92</accession>
    <name>A0A5B0ND92_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND92"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="90" mass="9985" checksum="C8E4F1C755C5186D" modified="2019-11-13" version="1">MEFPPSHGYICRSPLATHWRPLHNPYHLSHICASHLVMIPTNRSPVCSRNRKAPGSTLLLVSLLQTNVIISGHRASKPGPESSQSVIKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NE37</accession>
    <name>A0A5B0NE37_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032505</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NE37"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023151247" evidence="2">
        <location>
            <begin position="45"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11563" checksum="D66277A536ADC0F7" modified="2019-11-13" version="1">MSQPNCSSGIRRKRQHSPSVAWGVHHMRLMAILALIAILALTESQPVAKDGTSEKKSKDVAKKGIMKHDVERNRNERDEGMINEDGGNERSRQRDRLEVSSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFQ4</accession>
    <name>A0A5B0NFQ4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 13">Cytochrome c oxidase subunit 6, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Cytochrome c oxidase polypeptide VI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">COX6_2</name>
        <name evidence="15" type="ORF">PGT21_030825</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome c oxidase subunit 5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFQ4"/>
    <dbReference type="OrthoDB" id="2501130at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00923">
        <property type="entry name" value="Cyt_c_Oxidase_Va"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.40:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.40">
        <property type="entry name" value="Cytochrome c oxidase, subunit Va/VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003204">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036545">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200:SF11">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02284">
        <property type="entry name" value="COX5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48479">
        <property type="entry name" value="Cytochrome c oxidase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0349">Heme</keyword>
    <keyword evidence="8 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1087404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="211" mass="24021" checksum="9DA65BBC2371923B" modified="2019-11-13" version="1">MRLLTNQIERRTQPNRTTPKNTSVIFFHKTGSPQKPPKTIKESFTRKTTSTAEPTPIKMFFPAQLLIRRSLINPVPRLNLKSTGSSQKIIRQSSLITIQRTYSAGHAADESFSAFNERYTQFFEGVQDLFELQRGLNNCFAYDLVPAVPTVEAALRAARRVNDLPTAIRIFEGLKEKVENKGQYQAYLDELKPLRDELGVPTKEEMYGQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NM90</accession>
    <name>A0A5B0NM90_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NM90"/>
    <dbReference type="OrthoDB" id="2507804at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="596" mass="58324" checksum="8E5664E168900B33" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMX4</accession>
    <name>A0A5B0NMX4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="16">1.3.8.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">2-methyl branched chain acyl-CoA dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">2-methylbutyryl-coenzyme A dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">PGT21_005015</name>
        <name evidence="32" type="ORF">PGTUg99_036620</name>
    </gene>
    <organism evidence="32 34">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33 34" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31">21-0</strain>
            <strain evidence="32 34">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(2R)-2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = ethylacryloyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:65296"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:156439"/>
            <dbReference type="ChEBI" id="CHEBI:156440"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:65297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(2S)-2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48256"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57337"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:88166"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:48257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57336"/>
            <dbReference type="ChEBI" id="CHEBI:57337"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:43781"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>2-methylpropanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = 2-methylpropenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:44180"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57338"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62500"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:44181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:24005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>valproyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-propylpent-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:65344"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:156457"/>
            <dbReference type="ChEBI" id="CHEBI:156458"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:65345"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 27">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="15">Amino-acid degradation; L-isoleucine degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="32">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.5" evidence="16"/>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMX4"/>
    <dbReference type="OrthoDB" id="10262177at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003853">
        <property type="term" value="F:short-chain 2-methyl fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046395">
        <property type="term" value="P:carboxylic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01158">
        <property type="entry name" value="SCAD_SBCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000012">
        <property type="entry name" value="Acyl-CoA dehydrogenase short/branched chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase short/branched chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF1">
        <property type="entry name" value="SHORT_BRANCHED CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 27" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7 27" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12 27" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="30">
        <location>
            <begin position="62"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="29">
        <location>
            <begin position="177"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="28">
        <location>
            <begin position="286"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="KAA1070246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="EMBL" id="KAA1090153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="437" mass="48100" checksum="3223204979FDD617" modified="2019-11-13" version="1">MLTNPSRQVLLRTQQLLRSSKPTPRVLSSRHPTSALQRAFSSHPIARSQDGEPQPTSLYTFTEDESMLKDLAQRFAEEVVAPKVREMDEKETMDPEIIKGLFDNGFMGIETSQEHGGSGASFTSAILVIEELAKVDPSVSLICDVHNTIANTTIRNYASKALLDKYMPDLSTSKVACFCLSEPGSGSDAFALATRATKDSNGDYIINGSKMWITNSKEAEIFVVFANLDPSKGYKGISCFLLEKSMGVEIAKKEEKLGIKASSTCTLNFDNVKVPKENLIGQEGLGYKYAIEILNEGRIGIAAQMVGLAQGAFDKAVKYTYQRKQFGKPVGEFQGMQFQFADVHTEIEAARMLTYNAARLKEEGKPFTEMAAMAKLFSSQVAQNASGSAIEWCGGVGFTRETGIEKYWRDSKIGAIYEGSSNIQRQTLAKFIQKRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNW8</accession>
    <name>A0A5B0NNW8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019749</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNW8"/>
    <dbReference type="OrthoDB" id="2499804at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1090977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="136" mass="15283" checksum="F6E47FBEC2854393" modified="2019-11-13" version="1">MSIAERVPIIMANQTPLISWTADAVNDGPTSNTILIQWLATSNNYVRWRSGESKRAMCQEIIADMRLHGIHHRNYHSIMLRMTLLEGSYKQAYQWETTTGGFITAIGVDGHTTVSVRGKAASLFCLSCLRSHLIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQF9</accession>
    <name>A0A5B0NQF9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027427</name>
        <name evidence="1" type="ORF">PGTUg99_016292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQF9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="191" mass="20892" checksum="6A82710FB3071A15" modified="2019-11-13" version="1">MTAAVTLRRTHRLRCSSSLVAEIIKPGHSCTSFSSSVIHHYFLVSNTLEPNSSKHFSPKTIQAAPNMQSVLVAASLLCLSLLQGGLAAPTPLPTDQEFSKQAEPNVSLDKRQLMSYPGFANTFYNPYFGNRFYSGSSSGFNSQSYSDSVSNYSPWGSYNYLSQGNQMNSYSNNFSGLYAKDADHTKPTNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NT11</accession>
    <name>A0A5B0NT11_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_036406</name>
        <name evidence="7" type="ORF">PGTUg99_037572</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NT11"/>
    <dbReference type="OrthoDB" id="196717at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000029">
        <property type="entry name" value="CDP-alcohol phosphatidyltransferase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF77">
        <property type="entry name" value="CDP-ALCOHOL PHOSPHATIDYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1076259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1091634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="417" mass="47250" checksum="550B7255D6D0A91E" modified="2019-11-13" version="1">MDCTEFTRSEDYLDDQHLSRLKNYQYRSVDHSPTTKYILRHWWEFVAGWMPPWLAPNMITLIGLMFIVVNVITVIIYIPDLKADAPSWVYFSFAFGLFFYQTMDNVDGKQARKTGTSSPLGELFDHGIDSLNCVLGGLVQCAAVGTGHSLYAVFILIVACWPMYLSTWEEYHTGILYLGFVNGPTEGLLIAMAVLLCTGFGGVQIWSKPAENFIMLPQPILTYSEKYGHQLTLLDLFVSFVMFALVFGHAPSCFYNTWTAIRTNKSQPRSKQDLRILSFSQALLRTLPLLIFTFCSALWLASPHSHILKSEKIIEFGLMLCLIFGRIATRIILSHLTKSSFPFWSGMLIPIIGGSILINLPMIGLPVVLSPWRELVFLRLMFFFSIVGYVGSSLRTINRFCEVLRINCLTIRPKSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTQ2</accession>
    <name>A0A5B0NTQ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTQ2"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1092677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="14214" checksum="DF8DA0C5E114F5C7" modified="2019-11-13" version="1">MSITQFQTIEPLEEDLELLFQICTNVLRCQQCGHHDFLATIRVLLILTQKHSVAEAFLNSGVPSILLRSFLFKTETRGCLPYAAMLLRHTDESNSVKQAIMTKEITHFISSHGVVCQLCFKYQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVR4</accession>
    <name>A0A5B0NVR4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">BRCA1-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_012799</name>
        <name evidence="9" type="ORF">PGTUg99_032180</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVR4"/>
    <dbReference type="OrthoDB" id="273556at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16457">
        <property type="entry name" value="RING-H2_BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011422">
        <property type="entry name" value="BRAP2/ETP1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047243">
        <property type="entry name" value="RING-H2_BRAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007:SF7">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07576">
        <property type="entry name" value="BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="402"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="UBP-type" evidence="7">
        <location>
            <begin position="436"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="139"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="244"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="616"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="705"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="261"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="705"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1092746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1093846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="735" mass="81764" checksum="796A4AB8A88AA360" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NXD2</accession>
    <name>A0A5B0NXD2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CobW/HypB/UreG nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_002577</name>
        <name evidence="9" type="ORF">PGTUg99_005514</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. UreG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000115">
        <property type="protein sequence ID" value="KAA1129979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NXD2"/>
    <dbReference type="OrthoDB" id="10063137at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05540">
        <property type="entry name" value="UreG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000208">
        <property type="entry name" value="Urease accessory protein UreG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01389">
        <property type="entry name" value="UreG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004400">
        <property type="entry name" value="UreG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00101">
        <property type="entry name" value="ureG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31715">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31715:SF0">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0996">Nickel insertion</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="7">
        <location>
            <begin position="64"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1093911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1129979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="267" mass="28851" checksum="B5D71F5EE87D735E" modified="2019-11-13" version="1">MKFDLANGQPPNLSAESEHSHDHHTHDLAHDHGHSHEHLDHPGKFLERDLPDFTGRDWNERAFTVGIGGPVGSGKTALTLALCEKLRDRYNLGVLTNDIFIPEDRDFLQSHKALPNPSQIVAIETGGCPHAAIREDVSANLAALEKLQTEYKCELLLVESGGDNLAANYSRELADYIIYVIDVAGGDKIPRKGGPGISQSDLLVVNKIDLAPHVGASLEVMRRDSDVMRDGGATVFTAVKHGTGVEAVVSFILAAWESSGAKKLSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYP8</accession>
    <name>A0A5B0NYP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001375</name>
        <name evidence="3" type="ORF">PGTUg99_002262</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000051">
        <property type="protein sequence ID" value="KAA1134548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NYP8"/>
    <dbReference type="OrthoDB" id="2497483at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="316" mass="35299" checksum="85AC311610917C8B" modified="2019-11-13" version="1">MALAPDPVDNSGYIGRFEAATGAEFLSHRGKRVRYEDPVFFPSDGSTTTAIHLQQPDSEWTPVNRQEQDQTLSHGSTRRSSPPGEASGRPKRARDQPQEIGPPVVAKQDKPSRPSGSFSEQAFEKIMKAHNSRSFPYLKLADISPASVQLDSSIRLGQPIMGDYSQEINAYTGRYEVLIEDILETAHKLPNAYVGRKTRGWPATVVRPRHAEIEGEIILVLPTKDGDWRDAKSLVKQFKSLHRWIVYVHHLLLKNNNNPTSSSLQASYHQEMLLWLFGEVFNPKVGLPVIGRLDQASSNRQVFAPPPTLDHGVHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P050</accession>
    <name>A0A5B0P050_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichol-phosphate mannosyltransferase subunit 1</fullName>
            <ecNumber evidence="13">2.4.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">DPM1_5</name>
        <name evidence="16" type="synonym">DPM1_2</name>
        <name evidence="17" type="ORF">PGT21_033309</name>
        <name evidence="16" type="ORF">PGTUg99_011605</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="16 19">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl phosphate + GDP-alpha-D-mannose = a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + GDP</text>
            <dbReference type="Rhea" id="RHEA:21184"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 13">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="13"/>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P050"/>
    <dbReference type="OrthoDB" id="2603at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006720">
        <property type="term" value="P:isoprenoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000036">
        <property type="entry name" value="Dolichol-phosphate mannosyltransferase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="15">
        <location>
            <begin position="62"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1065250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1094947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="294" mass="33376" checksum="5613BB3CC1EDC4A1" modified="2019-11-13" version="1">MELLHRSNTGESCRPPNYPVHCPQSSGTNSNTTLNTTAQTRHILMIRSEGDMESKTNDDKYSVILPTYNERRNLPIMIWLLARTFDQHQIDWEVIVVDDNSPDGTLEVAQQLQRLYGPHRIILRPRAGKLGLGTAYMHGLSTCTGTFTIIMDADFSHHPKYLPTFIALQRAHGLDIVTGSRYRPGGGVHGWDFKRKLVSRGANMLTYILLDPGVSDVTGSFRLYRTSILRELIQKTTSRGYVFQMEIIVRARSMGCRMGEVPITFIDRLYGDSKLGKDEILGFVKGLWKLFWTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0U0</accession>
    <name>A0A5B0P0U0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026681</name>
        <name evidence="4" type="ORF">PGTUg99_016245</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1128969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0U0"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000164">
        <property type="entry name" value="Chitinase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033845140" description="GH18 domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="2">
        <location>
            <begin position="34"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1128969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="419" mass="45800" checksum="0D5AC76DA5A27651" modified="2019-11-13" version="1">MRSTSQSILGAFLFAALLFSTSTAAGRVKRDGSAKVKGYYPTYNSDAQPPSKIDWAAYTDVLFFMVIPEADGTLSFDPSLTRSQGRKLVKEFVDEARKHKVNPLISTGGWTGSRHFSNLTSSDYSRKRFAQILVNFSKKHGFTGIEMDWEYPNGEGIGCNSRRPADVINFGLLAKEIRTLWPEVELTAAVGVTGLIGATGEPATPKETAFLTENLDFINLMAYDVYGSWSDSTGPIAPIKGTCAGAGADQSVETGLQVALKQGFKASQILLGIPGYAKRFQLLSPKLLPKTVGKQTSFYYQKKSDVTPAGGKFDDKPGKDVCGNPQAWGGSFLVTELIKNGWLSKDQTRGLDGYKRVVDECSGQPFLANEKYLISYDDKESTLAKAKFAKQNKMGGVYFFDTMGPTDDTVRATRKFLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4C3</accession>
    <name>A0A5B0P4C3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoglycerate mutase (2,3-diphosphoglycerate-dependent)</fullName>
            <ecNumber evidence="4">5.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_014400</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4C3"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1096375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="219" mass="24780" checksum="8CCAEEC39DA8074A" modified="2019-11-13" version="1">MNTGETHAQIVLMRHGESEANEKRLFSWPAVPLTKKGCDEVESAATTLKGHRIAFDRAYTSGFKRAQDSLTITLKTLGYAKSNIPIEKAPELNTRTFGEWTGRPYAEARATLGSENVNHWFGIDYKKFPDVPPRGGETLEECDKRAQAYYTQEIHRRVLAGEKILVVASRNPIYSLMQKIEGDKLKEVPIDDMKTALPIIYQLDYRGNVLDVPFIIEHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5Q4</accession>
    <name>A0A5B0P5Q4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031549</name>
        <name evidence="4" type="ORF">PGT21_033192</name>
        <name evidence="3" type="ORF">PGTUg99_014337</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5Q4"/>
    <dbReference type="OrthoDB" id="2510514at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137660" evidence="1">
        <location>
            <begin position="27"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1113532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="473" mass="55390" checksum="C0007F4F08A4FBA9" modified="2019-11-13" version="1">MFIPSKLSRFWMIALCTSTASQMTIASLTVTQDTRIRPLEEMYSHIDNHVHDVASSDAVILKSPIGHSGQEQVLTLKSKEDLESMEADSRRISDSVEDIETLFGERYRPLVNTLKGLCCDVLVQEKPHKFMHAAGSLGPKYKFPKPIGADWVKTWKGLETNLIRFQKSWFDLQRHMEFLETVFCLGDYIIKYKLLPPNAIQEIDNSRQEMFSKWLQLYIHHEYNWSGRKHFFGRADSLIPTVKYLTSINSFEDFPRFLNALCLSPEKKNLIAYDSMKTIWQISNKKGKSDSPERQNFNQISSLFFRDNFIQEANELSGLLKTTSGNEKDHVLVRPDISFMMKHLFIIFEEPPKIFDDEDVKFQFFMSYPMLDFLNKNYQPIIQQFKSKKVDYQVLNKQLNFIGDVLETYHSYMQKTKAKRKLKYIQRVIKEFEEIPEEQSVIREWIEKVILQISQDTIVGLRRKLSVLNQVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8D6</accession>
    <name>A0A5B0P8D6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8D6"/>
    <dbReference type="OrthoDB" id="2506114at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="264" mass="29764" checksum="8C1B78FED93EB2B8" modified="2019-11-13" version="1">MSLTSGQSCKYLIYYVFICHSSVKDAQEKLDEVESTIPQRLQARISADGKSFETVVKSDEVYTLLAAFLYSRFCVPRIRLLRLFIFPKEGVPPEDRLKQLETLLSTSKLHFLALRYYPHNLSMHPLILYGLINTAVACALVLLINRQTHELVIDEDFYLSELHKIIALFDLGKKTVAATMSRKAITLLQTLIKQAELLAHSAESKSWDRRKKGPGATLQASHKSIAVDGEDQVKKHKQQVCIASLDSLSVKTHLPKKPCLVLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P931</accession>
    <name>A0A5B0P931_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012032</name>
        <name evidence="2" type="ORF">PGTUg99_020929</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P931"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1134045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="164" mass="17927" checksum="962479A78B09C89D" modified="2019-11-13" version="1">MVDRDGATVTFFSSLTPDEHLESCVSVQAEKITRLHGVESPHDQGDEHLLDRLAGKRSKITVVKNSFFDNSKDLPPLSTANWLFNSPRTPSTKAIQARLNSQATVHWLTSLRGSQHCTAAVITPESQMPGAAFGQRFTISSMTVQIGIAEAEGALLSNRSEQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFF6</accession>
    <name>A0A5B0PFF6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exocyst complex component Sec10-like alpha-helical bundle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFF6"/>
    <dbReference type="OrthoDB" id="5554140at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="1">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="138" mass="15110" checksum="138C4798F3E3F4FC" modified="2019-11-13" version="1">MSRLLCKHIKRTKISIEGAHQLETDIARYAHVLGSMAGESKEAADVGLHVSIDALRLVGRLFGCKTPAQLVRFIRDLGLGKVSSSSTHPQLLLGSGVLPMSIDELYQFVQARDDWNRIQIVVEAELFGIGVGDRCVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFM3</accession>
    <name>A0A5B0PFM3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFM3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7857" checksum="06E41DE10E3B34EB" modified="2019-11-13" version="1">MSYFVDRSIDDFYFRDTPTWQQAKLQPTSTTATTTTTTTTPPNENQELIKYNIGGLQLTTDSHSTDSYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFN9</accession>
    <name>A0A5B0PFN9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028499</name>
        <name evidence="4" type="ORF">PGTUg99_014146</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000144">
        <property type="protein sequence ID" value="KAA1128132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFN9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137746" evidence="2">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1128132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="53" mass="5764" checksum="A9782687EE9B38FD" modified="2019-11-13" version="1">MHIPLILLFGILAVSQTGAVSTSPVEKLESEENGRQPCPHVPKPHCSRCPVRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHC5</accession>
    <name>A0A5B0PHC5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHC5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022893615" evidence="2">
        <location>
            <begin position="22"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="12096" checksum="6E129F51D6A3F410" modified="2019-11-13" version="1">MKSISILLIVCALACFSASNALVLRSLSRSLSFDDLKLYNTGTRLPRPNTQAFPAGLPLRPHNSLNRDDDVRLPHHPPPAPHHQSGGPGGLHAGRNWGASLTPSGEDEGHTYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJV8</accession>
    <name>A0A5B0PJV8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJV8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1100179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="151" mass="16971" checksum="8EBBF44183322B01" modified="2019-11-13" version="1">MDIRKLELRLSPSLFTDRSADTSLLATPVFKGRKAHRPSPCDPPSARNHIFTCKIFYCKQSPRAFHLHTFNPVQSSKFFLQRKKKNQRHPPAQPSSASILDYGPIKIKLQIYLLPSARRSFASSITHSIEFRHSVGSGSTSAAWPPLGTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PK25</accession>
    <name>A0A5B0PK25_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_050194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PK25"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="214" mass="24951" checksum="D7F336F191AC0C88" modified="2019-11-13" version="1">MFSSQDYFNTYRYIHGSHGSVSYEKESAQLGNQVLNDRKGFIDTTKKLIHQIFSHSGISFEFKYHLTERQSAVCVLVFYLLGFIERELCPGIIKELIDEISFPHQPLWKAQDHITEIIQLVFLTSRYNILEKIAVEYWDITKNGNLIQDSPYKESIYKGYLKELAEVLADFKLVNLRFSPTDTKLIKFDKKFLSNIQRINQDGDAHLNDLMGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLX1</accession>
    <name>A0A5B0PLX1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serum paraoxonase/arylesterase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLX1"/>
    <dbReference type="OrthoDB" id="5307922at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051288">
        <property type="entry name" value="Serum_paraoxonase/arylesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799">
        <property type="entry name" value="PARAOXONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799:SF12">
        <property type="entry name" value="PARAOXONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="46732" checksum="A503D1DCC4F22B0B" modified="2019-11-13" version="1">MVESGSIILGVVAILSALFRAFLKPKFDQAGIFRTGTNRNNEHCSTIGLEACEDIWVDQPTGLAYLACSSRTQRAFWETGVGILEAEKLPPRSLDHITILDLKTHEHRKVELVNLPPHIVKNGIYVHGIDLVVTPSDPTDSTAANQMPFSDGAAGDQLGSRRATIYLINHRPPSDRKSAPLIGAQSVIEVFDTVLGDSKATHTTTIEHGLILTPNNLVGISETSFYVTNDHSSKTGWTRRLEPFFTDSEIDSIIFCSFQDELNCLPAVSGKHPHPNGIAKGPGDSLYMVTTFDPYLRVYEIQADYTLALTDELKLPRVVDNIYVTPKGSIFMAGIPSLYRFKKRLLGMQEGNFDILSPSDVWKVSNETSSDAFYGGRLKVEQVLADDGNKINGNTGVAVWDSKLYLTGLTSPYISVCKIDEELAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNS2</accession>
    <name>A0A5B0PNS2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNS2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022942807" evidence="2">
        <location>
            <begin position="16"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="180" mass="18794" checksum="6C7A428EE608B3DF" modified="2019-11-13" version="1">MKLICLSLLVGSIAASPALRLGVHNPELTLPDLPAESPTLLVPYGIEEDGYPNIDPSWIATLGKSSPVLICDHHAKPFVAEQPSIIHKPDSLPALKEESRKDGGSAEPLEGGSSTDAGKLPALLSVQHAKASEAEQPSEVLPAPKKELRKHDGSAQPLEVAKSADGQGPRGGESSTSSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PP36</accession>
    <name>A0A5B0PP36_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002849</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1102981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PP36"/>
    <dbReference type="OrthoDB" id="2495994at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1102981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="177" mass="20581" checksum="53D075B32EDC2908" modified="2019-11-13" version="1">MVLIKRVASLSGVSSRGSIFSFKRFHRFRKILQDMKAQLTAQETANREDAFKKVRNALEADDYWTAALEMSRFEKLHGRLNNVEMHWLVNYQNHIHEQLKNHVREAVVVSMDQARGDFRDWLNLGIPVEVVAAQSEKAHRNSVEIQTHLLGIKSWTRRAGFLSVIIKKGKQEESIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQJ4</accession>
    <name>A0A5B0PQJ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1102963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQJ4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022682648" evidence="2">
        <location>
            <begin position="28"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="381" mass="41492" checksum="98210938B86DB243" modified="2019-11-13" version="1">MSTPQRSLKRFSLHLSVVCLFILAVPAGCPVAKPGVTDRMTTVTYMPDASSKELIATGGTGALVQLPKGSLIRFRNESGSSSSHTKVKTNLDKILPGGDRVEPGPLLVKENGDEKIGEIPESSILKTQITSGNPNALENQNGKTRNGLSKIPVQSVSKPPSRPAPGVPRVPPPTRAPPELPASHVLQVTRQRSSEADMKTEIKPDIPHQKPSSLSNANTVVNEIQNGAVNHKLETQGGMKTQSSNLKMAKEHTAALVDRTYSKTKSSKQPTDSRVSQDQNGSLIYKTVKNTENMQPKLSPGVISQNIPATKSDSIIPNGVILDLEIFHAIQNSFEKLRGETNILEGKYPKSHQKYWERMNGYFENADHSKRDYTSTRGLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRB7</accession>
    <name>A0A5B0PRB7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRB7"/>
    <dbReference type="OrthoDB" id="2507571at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="498" mass="54235" checksum="6E59BA3DB381AB3E" modified="2019-11-13" version="1">MPPTQRLSEYPPPASQAPSVFCNWPANPQAPNGPAVSTPTAWNDSSPNQDQPRLSLAHHHGPTLAHQVLPVEKGQQLPTLGLVSSGRPPLPRSRMFQCDITFLIYCAKKNKQKKTVWTSLKSDVDMSISFDCQTINLSQFQHLVSTECTGPYPKLPELLEHCTHSSPPSIRWVGYMGRNPNWLKTGSQSVADPPTFLAWIQEIIKSGVKKGGVYLKMANPSDGKKLAASNDSIAATVRRHEAQVATARAAFGTQPLPSGSAAPSESQAIGQGLFDLNEDAEDSCDESFDARKIIEEDIFKKYVGNVGVDVNHTVYPHPTDINRYIILTSGNVASWARAIQAKEKGVSLTSPPSCLKYYHRKGRGSNAWQARGGPQSSDRPSGYSGAGVMTPEMASEIVEICHQTLARKRAHSPSLSVSPVGVARGEGELGEYLEFAGIAKKEETLGVLARHGVDSYDMFQDGFMTPEQLKVLGLPVGTLAKLCRNVARYERSLARPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSS7</accession>
    <name>A0A5B0PSS7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1102999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSS7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022879204" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11401" checksum="546A63BD4742C5BB" modified="2019-11-13" version="1">MRHFGLNFLVVSLSLSQAHGNQYQPSDASSYQTAAPATVISLMRSQTAAGKLLNPSPLRKLPQLFNTIVKGAATPSQDRLNIDGCFNQCYFKQHQRCGQQDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZF5</accession>
    <name>A0A5B0PZF5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GmrSD restriction endonucleases N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032528</name>
        <name evidence="4" type="ORF">PGTUg99_029216</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZF5"/>
    <dbReference type="OrthoDB" id="5419821at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004919">
        <property type="entry name" value="GmrSD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39639">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39639:SF1">
        <property type="entry name" value="DUF262 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03235">
        <property type="entry name" value="GmrSD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GmrSD restriction endonucleases N-terminal" evidence="2">
        <location>
            <begin position="52"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1106283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1109334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="633" mass="70774" checksum="5721A6092F2C8B58" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0Z3</accession>
    <name>A0A5B0Q0Z3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036903</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0Z3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1106664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="186" mass="20903" checksum="CBE0ADFA104C1DD5" modified="2019-11-13" version="1">MKDQPRAERFSARGLGLRVRLMGNLELKAVQLESTMSWEVFSLRSPVALTEEGGMDLELICLQLELEVQSSCDESGNPGSCSPPRLHHKSLAATLIRPISLDCHQSGGPRTWTIREGKYDYTQLTKNNYNYPFGLDSPHTIVDLARDRLRSGFRLLTAGPRLFRSVFCLLAAGSRLLRSFDFGRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5K1</accession>
    <name>A0A5B0Q5K1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5K1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="85" mass="8568" checksum="20256275EA808D47" modified="2019-11-13" version="1">MVFTERLAINSASGSIQAHPAQSALGPSSSSSVSSTQLPDPAQSALGPSSSSSEIQLIQLWDPAHSALKSSSSSSSSSGIQLIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q612</accession>
    <name>A0A5B0Q612_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020189</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q612"/>
    <dbReference type="OrthoDB" id="10312465at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022934743" evidence="2">
        <location>
            <begin position="19"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="65" mass="6728" checksum="B8BD03BD46982FA7" modified="2019-11-13" version="1">MMHCSIYLALIFAAVALAAPFESSISPSQYSGEGSGIGGRKFDAARSGRPGMRQNMMGNSGSLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6Z9</accession>
    <name>A0A5B0Q6Z9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6Z9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="11305" checksum="7DAB7FB3ED8A85BB" modified="2019-11-13" version="1">MTKQLPVYMQKLRWLKKKKEKIRSKQPPTPFDQKALPERELGKQHQHLSINQLFRVSIRAPHQITSASPAQPTNNDPHTSLNTLSRPPSSLWSTKLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9L7</accession>
    <name>A0A5B0Q9L7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9L7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="128" mass="14747" checksum="984DF49588783F95" modified="2019-11-13" version="1">MQPQQYKCTTLAHIDMTSPDVSHQIQVSREQIKVELATTPALSQRQQAPDIILTNHHTPPRPPLPQSNTTTKSIIRQSSTASLALETLTLHNSLAIRVRSQVDHQLWQQDQLLNRIRWWVWDVFVRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA13</accession>
    <name>A0A5B0QA13_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_009604</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QA13"/>
    <dbReference type="OrthoDB" id="406631at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000251">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF39">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022684065" description="mannan endo-1,4-beta-mannosidase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="11">
        <location>
            <begin position="209"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="67"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1110080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="624" mass="69643" checksum="188111772160ED4D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC81</accession>
    <name>A0A5B0QC81_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QC81"/>
    <dbReference type="OrthoDB" id="10419925at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="83" mass="9769" checksum="D01AA123244C289D" modified="2019-11-13" version="1">MLNMSPFSPQATHLWQDQTSSKFQTQTERSARRTTNWHKIANPNNVAHKWCKQFRPTQHRNGRGTHKFWIKHACKADTQLLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCL1</accession>
    <name>A0A5B0QCL1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCL1"/>
    <dbReference type="OrthoDB" id="10404827at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="154" mass="17405" checksum="9C5AA4824A585DC8" modified="2019-11-13" version="1">MNTRPTQTTSAPRRLPAGFPGNTFDHPFVIDRPTGFPGNTFDHPFVIDRPTGFPGNTFDHPFVIDRPTGFPGNTFDHPFVIGQIHIYCTDAAFPRSSTTETAVEEAERRHRNDFVVYHRMASMAANIRLPRIAPYTEVPESSITIDQRQIHRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDT8</accession>
    <name>A0A5B0QDT8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QDT8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022982739" evidence="1">
        <location>
            <begin position="18"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="122" mass="14003" checksum="F2D3040625C42760" modified="2019-11-13" version="1">MKITVVWFMQLLLHGLACTLIPIEKFKNANGPPHGFTRLTTVSKPTEATRLFWRTPDDSPAILYTSILMAGNIHFYNEQQGIKYCLRDPASNLWTDVETLAPQKNIWLHSTIDSIELYVQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QF74</accession>
    <name>A0A5B0QF74_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QF74"/>
    <dbReference type="OrthoDB" id="2506469at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="617"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="643" mass="72166" checksum="DCEA1A46C85C9546" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNC3</accession>
    <name>A0A5B0QNC3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNC3"/>
    <dbReference type="OrthoDB" id="10285034at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="10731" checksum="802FAD80C1777F1C" modified="2019-11-13" version="1">MDTAAGVDHARYALRPGTATRRPLDRPIEAEGRACGLWASVVLTTQRQENQQFADSPAGASGSAGGEVPWKRARPGENSPPALPTLTRPAWVLARKSQQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP42</accession>
    <name>A0A5B0QP42_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Choline/carnitine acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_028718</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP42"/>
    <dbReference type="OMA" id="HILVMRR"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000019">
        <property type="entry name" value="Carnitine acetyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000003">
        <property type="entry name" value="Carnitine acetyl transferase FacC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF29">
        <property type="entry name" value="MITOCHONDRIAL CARNITINE O-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="7">
        <location>
            <begin position="18"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="551"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="665"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="721"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="766"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="721"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1114982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="793" mass="89267" checksum="098CE66C7E5D6D1D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPC0</accession>
    <name>A0A5B0QPC0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">QCR2_2</name>
        <name evidence="13" type="ORF">PGT21_024521</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPC0"/>
    <dbReference type="OrthoDB" id="6369905at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000021">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000039">
        <property type="entry name" value="Ubiquinol-cytochrome c reductase core subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF209">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="12">
        <location>
            <begin position="41"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1114823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="439" mass="46747" checksum="5CBCFEC10D51C81F" modified="2019-11-13" version="1">MLLSLSTRSSLARSARSYAHQAAAPLTITANQAATAQTVLTTPADNKLTASISVFIKAGSRYQPSHGLAHLLKNSVFKSTQKRSALSLVRETELLGGILTSSLTREHLILSAEFLKGNEGYFAEVLGDVISCSKFTRHEFHEEALPGAQAEYEQAQTDGSIVALEQAHQVAFRQGLGNPLLMDPKMGGSQEMMEEYGRQSFGRAREQTIVGTGIEGGRLTELVEQFFGSSSGEEGSGGSGGSGKTSYHGGEARIARGEDGSRGRMVIGFKGTTAPEYTVLQHLLGSEPGSIKWAAGSGPLAALPVRAFNLAYSDIALFGFLVSAPANQTRSVAQNALRQLRQIATGNTVDHEAVKRAALKAQFLVASHLENNLLRTELLGTQALGSPKSASQLTDLYSSYAQVTADQVVKAAKDLLDSPPTTVAVGNTQELPYFDQLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQG5</accession>
    <name>A0A5B0QQG5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQG5"/>
    <dbReference type="OrthoDB" id="10262250at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022826935" description="C2H2-type domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12978" checksum="5A38852D12EFE9A7" modified="2019-11-13" version="1">MRSPRVWLVCALSMCFNGDTATLPSEPRPEICVFCNRDAVIRQCHEVTYMTAFKTITKTCENLPAGGCDKRIYFHQYTCGKCGNYAWINNDPCPHHDHIQELESPNDYECAAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVJ5</accession>
    <name>A0A5B0QVJ5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Chromatin modification-related protein EAF7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_028303</name>
        <name evidence="9" type="ORF">PGTUg99_031393</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVJ5"/>
    <dbReference type="OrthoDB" id="5595141at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012423">
        <property type="entry name" value="Eaf7/MRGBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13581">
        <property type="entry name" value="MRG-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13581:SF5">
        <property type="entry name" value="MRG_MORF4L-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07904">
        <property type="entry name" value="Eaf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="199"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="279"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1098096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1116935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="35700" checksum="389008AE1A49D120" modified="2019-11-13" version="1">MASSTAEASLQPSELLSTTDGETAFFRSIIRYRPRGSNRHFSMVGICKDLERELQTVIPSDEIWNTLRTCYDLDILAEMEPDEPDEIERNKSTVELLRSKQHTFFREFQLPIHPSIPAEQSFQSLIDERRLESSSARSSPEQRSPGMVSSPRRRTTANSLKLHLDRSGQSSSSKYLNPSSAAKSGSDHRIGDPTIQEGMESDLTEQEDYEDDDDEEEEDKELEGKDDEEEEEEEEEDEPEPEPRTKKLKTSNRTDSSHHHRPPTTSSSRSRNTGKSNKPSSTTASTSTPTTTATSINNSNNTPSSSRSRTRGRWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVL0</accession>
    <name>A0A5B0QVL0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U3 small nucleolar RNA-associated protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_004156</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVL0"/>
    <dbReference type="OrthoDB" id="277439at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006709">
        <property type="entry name" value="SSU_processome_Utp14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150:SF12">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04615">
        <property type="entry name" value="Utp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="122"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="544"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="603"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="692"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="731"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="544"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="613"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="749"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1117342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1062" mass="117975" checksum="0163E0AF56BD7625" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWW3</accession>
    <name>A0A5B0QWW3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWW3"/>
    <dbReference type="OrthoDB" id="10313120at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="260" mass="29377" checksum="2C2F1B0AB0C976D6" modified="2019-11-13" version="1">MPKDIVVTLNLQHNCHDGKCPIKKTKMVQAERQDTPVRVQQVCHTDSKHYILNSVSFHESEEHRHMNNLIFHQIDSEDVVEAMSEGHLTWKAHCQKTMPRKKKKVGVRTGDDNQSCKPLVELSAISFGGKLESWSRVGERTDHAARFRSPNSGRFCWVNEIGLTTSVARERPTDSQTVLPTALQPTRSVNLGGWGGPQCTNGHITWPFSHCGPPHPPTNGLYRGWRIVVHPGWFHNHPPTENVARWCSVGKKSAPVPRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1K9</accession>
    <name>A0A5B0R1K9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1K9"/>
    <dbReference type="OrthoDB" id="2506207at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="556"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="821"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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            <begin position="478"/>
            <end position="496"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <end position="514"/>
        </location>
    </feature>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <end position="584"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1004"/>
            <end position="1016"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1085" mass="116336" checksum="00DA84323AE7E4EA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1T1</accession>
    <name>A0A5B0R1T1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Puccinia</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1T1"/>
    <dbReference type="OrthoDB" id="2506620at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="224" mass="25176" checksum="5ACA114E22E272EC" modified="2019-11-13" version="1">MSKEPLNPAQKCSYKMRIEMLDSYLTGKKSSDVSSHFKPGHLVIVDLRDPFTNSSLVIAMFEIIVGLFVERHMETGKVLLLDEAHKYLNSDQCSARFTESMTSLIRQQRHFGMRTIISTQEPTVVPDAIIDLVSFLVLHRFSSPTWIKHLSKHISVDQDPETDLDWSRMISRLALGEAVIVAPTALSIKSDSISSEPLATGFFITRTRKRLTYDGGASIVAYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3X3</accession>
    <name>A0A5B0R3X3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_037164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3X3"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="term" value="P:regulation of cytokinesis, actomyosin contractile ring assembly"/>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1120109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="416" mass="45743" checksum="040D8F324D1DAB1A" modified="2019-11-13" version="1">MLKKMSSNDPARPGLLACIATCNRLAVCELDDHLIKAATMWGLHRSIDHFPAILVKPGRYFIDSIDVLDIIPDTPSPTVLHCTLFLFNDTIVIAKKPANGQLTGRTLAGLDDIDRLVGAMKKAKTNNSSLNSVVSGGTDFFAKSLGGGHSTPTKLKKGSMRFKGLVDVHDVIVANEPGQTGIGSTSEVSFDLYLGRPPQDVSDRWTDRPFRHYVVCPPPAPALSGHEKSLSLSAHPSSSSTSHHQPPNPTSHSLSHKSLQNLNSQQSYAAALAERDRFLDNLRKSQALVKAADDRSTVMRTKFVNEIDNKAIIDSFWNLYDKQSYLTEQRKHRVVLQLVGTDAVDPLQFVTESIDPAPPLMIIRAHFNDPDDPDCRVHVRRKPNHVFPSLNASIDKAEEDIVVQTECLSGLIVRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R424</accession>
    <name>A0A5B0R424_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">V-type proton ATPase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">VMA7_2</name>
        <name evidence="10" type="ORF">PGT21_033635</name>
    </gene>
    <organism evidence="10 11">
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        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Vacuole membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R424"/>
    <dbReference type="OrthoDB" id="10261947at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005772">
        <property type="entry name" value="ATPase_V1-cplx_fsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01101">
        <property type="entry name" value="V_ATP_synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861:SF2">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015945">
        <property type="entry name" value="ATPase_V1_F_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1119765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="150" mass="16582" checksum="0752A823E21B075B" modified="2019-11-13" version="1">MANARLGGSKRTLIATIGDEDSITGLLLAGTGHVTAASKKNFMVVDSSQYLTTFNILSFIHQDFKGSVLRSERGTETPVSEIQKAFDEFTTERDDIAIVLINQHVADKIRPSVDKYEAAFPALLEIPSKDHPYDPEKDSVLKRVKKLFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R454</accession>
    <name>A0A5B0R454_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_050284</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R454"/>
    <dbReference type="OrthoDB" id="1607513at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="10">
        <location>
            <begin position="94"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1119795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="295" mass="33224" checksum="90EC212113CFE9F6" modified="2019-11-13" version="1">MPRGRKHRHQLKAPMSTSSIAKSNGTTSAPDEDNNSSESDVEVTANPPTLPESQATSDATTPTPRNHSVNTQIKKKKNHQESSTETEPTNKKRKTTSEVWTHFKQYGSGDKLTAVCCSCQAPLTGKSASGTTHLWRHLERCSNFKNKTKQALLKTTAGTTTNWSFSQEESRKLLAKMVIAHEQPFTLVDNPLFQKFLASLQPKFRLFSSTTLRSDVMKLYESMKVDLAREISQADRIALTTDLWTSRNQTPFMVVSAHFILPDWILKKRIIAFKELPTPHMSATEYMSQYLSCTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R954</accession>
    <name>A0A5B0R954_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016774</name>
        <name evidence="2" type="ORF">PGTUg99_032040</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R954"/>
    <dbReference type="OrthoDB" id="2508708at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1122177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="422" mass="49421" checksum="93C2F9CB402792C4" modified="2019-11-13" version="1">MQIVFQFVSFQVYTHTRHKIQTSTRMNDSSSELSQPDIPEEQKLELRRQADLVIQDFRALRKYPFSWPANTQPDISIERLRSKKDILTRLDSTLLPRLRQQCASLSGLLCSRYHLREDLASTLQLTSEIQANLHLTLGQTIEALNEMFPGRIPEPCQTDDQHFEELKIYRLYCFNSSIRKNLKTTLRLFFELSGYIIKDFKLSKNHSPEYFRIISFTLDEEIASAIGFLKESELSLIWDMWKNAIGFYDDELEGLLGQLDPDRILFEDDDEPLSEPAVELGTSLVPIFKLSRLFFKKLYRQRIKKKEVNLFTEMCSNELLSLDRLTKDIHKSLYTMWYSIQDADRIVPINTSKAIMNEIVQILTHFQSYLSLIYSYILPNLFPNVIELPSQNHFCHWYATWTVSFLRAIINAVQVAITFVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RDZ0</accession>
    <name>A0A5B0RDZ0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Uridylate kinase</fullName>
            <shortName evidence="9">UK</shortName>
            <ecNumber evidence="9">2.7.4.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:UMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deoxycytidylate kinase</fullName>
            <shortName evidence="9">CK</shortName>
            <shortName evidence="9">dCMP kinase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Uridine monophosphate kinase</fullName>
            <shortName evidence="9">UMP kinase</shortName>
            <shortName evidence="9">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CMPK1_2</name>
        <name evidence="12" type="synonym">CMPK1_1</name>
        <name evidence="12" type="ORF">PGT21_025497</name>
        <name evidence="13" type="ORF">PGTUg99_012078</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and dUMP as phosphate acceptors, but can also use CMP, dCMP and AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <text evidence="9">Predominantly cytoplasmic.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adenylate kinase family. UMP-CMP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.14" evidence="9"/>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000208">
        <property type="protein sequence ID" value="KAA1123448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RDZ0"/>
    <dbReference type="OrthoDB" id="442176at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019205">
        <property type="term" value="F:nucleobase-containing compound kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016776">
        <property type="term" value="F:phosphotransferase activity, phosphate group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009123">
        <property type="term" value="P:nucleoside monophosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000315">
        <property type="entry name" value="Adenylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03172">
        <property type="entry name" value="Adenylate_kinase_UMP_CMP_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006266">
        <property type="entry name" value="UMP_CMP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01359">
        <property type="entry name" value="UMP_CMP_kin_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="9">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="9">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="200"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="20"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="68"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="98"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1099936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1123448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="250" mass="27480" checksum="28BE4442D4F83C1E" modified="2019-11-13" version="1">MAPVFENKEIIVVFVLGGPGAGKGTQCTRLVAEYGFTHLSAGDLLRIEQQRDGSEYAPVIQEHIKEGKIVPMEIILKLMQESMGRSIEDGHTKFLIDGFPREMEQAIKFDAEICESALVLFLTCPEEKLLPRLIERGKTSGREDDNEESIKKRFRTFVETSMPVIDYYSSKGKVVQIDSSGTVDEIYKDIQAALDAQLASHPSFPEPSEPSTSSSTSAAASSVAGEVSVPATRNIKPMVPGETPKPRNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RM31</accession>
    <name>A0A5B0RM31_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Protein transport protein BOS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">BOS1_1</name>
        <name evidence="14" type="synonym">BOS1_2</name>
        <name evidence="14" type="ORF">PGT21_020725</name>
        <name evidence="15" type="ORF">PGTUg99_035015</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">SNARE required for protein transport between the ER and the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 11">Belongs to the BOS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RM31"/>
    <dbReference type="OrthoDB" id="158360at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15863">
        <property type="entry name" value="SNARE_GS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF1">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR028865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1076826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1126991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="251" mass="27711" checksum="78AE603F5A86E592" modified="2019-11-13" version="1">MNSLYSLALRQTSSIQAELTELEKSITSSTTRNNTAGLHGQIAASLSSLQRTLDDYQAMSKAEVVDSKRAKAAARIEKFKDDFLALKKQFDQIKTLERTLKEESQRQELFQSNSTSYRTNAATHSSPTTAESPYQTGYHRTNAAINSRAGGGGGAGYRTNSALDENHFLQQTNSTLDIYIAQGQAILGNLGDQRDMLKGTQKKLRSAANVLGLSRETIQFIERRSKADFILFGIGATFTLVSFYFILKLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RQJ1</accession>
    <name>A0A5B0RQJ1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001982</name>
        <name evidence="4" type="ORF">PGTUg99_007151</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000147">
        <property type="protein sequence ID" value="KAA1127897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RQJ1"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF6">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="231"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="618"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="898"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1127897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="920" mass="103533" checksum="5C830FCF2DCACFE8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RU99</accession>
    <name>A0A5B0RU99_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021842</name>
        <name evidence="3" type="ORF">PGTUg99_031291</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RU99"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="75" mass="8481" checksum="8DBE99F22A989CCA" modified="2019-11-13" version="1">MIDKKSETFVKKYEMKLDEIESDIAAMKKSVDKIESLCITHFTTCSINPINHLPKVLVDETDPAHFIKLPGDGCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RZ45</accession>
    <name>A0A5B0RZ45_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">W2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_027806</name>
        <name evidence="9" type="ORF">PGTUg99_005483</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000119">
        <property type="protein sequence ID" value="KAA1129854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RZ45"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11561">
        <property type="entry name" value="W2_eIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000031">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="7">
        <location>
            <begin position="246"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="150"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1104603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1129854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="405" mass="45025" checksum="BC14D3A07F02F469" modified="2019-11-13" version="1">MAVGIVNIGKDARGDMFHRYKMPVLQVKVEGKGNGIKTVIPNMSDIARALARPPTYTTKFFGCELGAQTTFDEKYDRYIVNGAHQADRLRELLDVFIDKFVLCSSCKNPETELMIVGRDELIWRDCKACGHRGHVDNRHKLCTFILKNPPESSKKKSKRDKKAAKAGEAGSAPVEGGSSDDGHNSQDELTKRIEGEAVDEKTAEATSTLKDDDWSADTSAGAVKARMKSLEAGIQNGLSLADEDDEENPGGPYGIFSEWILTNRDSVSDPEILVKAKELGIEKKHKTVQILAQNLFSENAVKEVPKHHALFAKMVNSEKHQKALLGGIEQLCGEAYPSLVPLGVPKIIMEFYQADILEEDVIKQWGTHVSKKYVDKEVSKRVRRACEPLLKWLDEAEAEDDEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SCT8</accession>
    <name>A0A5B0SCT8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DUF788-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_002835</name>
        <name evidence="9" type="ORF">PGTUg99_026860</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM208 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SCT8"/>
    <dbReference type="OrthoDB" id="2505843at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008506">
        <property type="entry name" value="SND2/TMEM208"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05620">
        <property type="entry name" value="TMEM208_SND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1076333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1135620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15664" checksum="56904711B25F2010" modified="2019-11-13" version="1">MAKANLKRLSDSNRGVLQMLSKLLIGSNAIHLLSILVFPSQKMMSRYGLYYLISSVLSYLSYSFLSATGSPQRTGGGATQTPDDLSTGIHQYIVDYCYISVFVWLTTGLISKSFWMAYWIIPLYGLYKAFSIARRLFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJE0</accession>
    <name>A0A5B0LJE0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJE0"/>
    <dbReference type="OrthoDB" id="2499245at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1064435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="257" mass="28357" checksum="C0046E184F76675D" modified="2019-11-13" version="1">MDQVTQPRSARAGSHILAPNLATFFAPPKIAYQRLWAALGQAWGIRRLANVKNEWVQQANDDNNGLYTAYYHFCQSYDNLGASLNDPAGDGASRSAMELVQNFCGAMAGLAFICTKNNMKAKQAKLDKLRTVHHVQHVQMLANLLIFGPTATFTSLHKGPHMTLSNANALLELGSSVLKQKIALGITTPMPHSTLDNLRIHFLNFLIKMGFNQLDNDGVPQVNWKDVHASFYNDFSPSVIAGIFSQDIEIALAHRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKD1</accession>
    <name>A0A5B0LKD1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKD1"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="50" mass="5777" checksum="A557DB6AD5B97470" modified="2019-11-13" version="1">MVVRQTSVVKNSHPSEPREAIKGQFRLPSIKPLSRLNTRHQLQGLRFLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LL47</accession>
    <name>A0A5B0LL47_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_009941</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LL47"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051732">
        <property type="entry name" value="USF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117:SF3">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="319"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="243"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="418"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="168"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1064630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="580" mass="64836" checksum="FB6256FAC9185E45" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQB6</accession>
    <name>A0A5B0LQB6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019177</name>
        <name evidence="3" type="ORF">PGTUg99_015599</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000110">
        <property type="protein sequence ID" value="KAA1130316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQB6"/>
    <dbReference type="OrthoDB" id="2506854at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="635" mass="72002" checksum="9DB3C19EC28FFEE4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LT05</accession>
    <name>A0A5B0LT05_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1083891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2505938at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="137" mass="14532" checksum="6290DDE8F4154304" modified="2019-11-13" version="1">MPPSLPPTLTTVTLKFDKTTVLLPATKKTRVSELKTGFLEALKGTKTSPSNGSLEDSSCFQIFRASKTAANLTEAGSAGGAGGGPADSWDALADDHATMSDLALEEAETLGIGFKNSKGSYDQPLIEIWREQETRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXL7</accession>
    <name>A0A5B0LXL7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXL7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="106" mass="11758" checksum="0802B2B59C909F01" modified="2019-11-13" version="1">MERMSGSLHDHEGINREDETPSKTFHREAGSHSAPSAQRLGSKSPPFNFIYPQPQGPAFKPQPRQQSFTPLSTTSSTTTSSSTITTFNRYTNLSTASSRTILYHNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M183</accession>
    <name>A0A5B0M183_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M183"/>
    <dbReference type="OrthoDB" id="10325491at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="480" mass="54942" checksum="800D4F462F974421" modified="2019-11-13" version="1">MQIEPVVSGQRSSRKTSSRTAAAKTIMLILCNSAIYLNILIVILSSPNGVVGPAVPNVVKDTEALHATSDSSLLHGAGKPERNNIISGSNHDRFQAERPRHEQGAHEASGDEGEALDEGAGFEGINEDKLLERIRHQDRAVEEHKETIKQHGVTEANRLHQGMKNDFPPPEEAASWNAAQREHRLTRWWSGIVNFWRSIFRRKQWKQDKLYLQLKTLRSRMEEDTPKEVRKAINRLSILEYKGFEFSKEEVKLTNKLSGQMKRKSFQLDQADHQLINQIGWKKVVGRKVEEIDELLKVYTDAISKSRFRKRYVNKYKEALENLRPMLVQDVTRWNPEYTKLTESIRKLKDGPDFPKNLAELDPETEQRLALLDSEKRRIQKYYPQFYPQDIIKGLAEKFAASGIEMDPKMEEMLASMLKGGGGALPEELAHLLEPPSSKFVILQNLVDSYNENKTFCVVRMFIESVHAGSNISSRELVMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M599</accession>
    <name>A0A5B0M599_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit POB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">POB3_2</name>
        <name evidence="14" type="ORF">PGT21_006105</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M599"/>
    <dbReference type="OrthoDB" id="498543at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.220:FF:000003">
        <property type="entry name" value="FACT complex subunit POB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000514">
        <property type="entry name" value="FACT complex subunit POB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.220">
        <property type="entry name" value="Structure-specific recognition protein (SSRP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038167">
        <property type="entry name" value="SSRP1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022820398" description="FACT complex subunit POB3" evidence="12">
        <location>
            <begin position="19"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="13">
        <location>
            <begin position="371"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="476"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="478"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="547"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1071403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="587" mass="65255" checksum="BD175A2769E912A7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8W4</accession>
    <name>A0A5B0M8W4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_000196</name>
        <name evidence="2" type="ORF">PGT21_022970</name>
        <name evidence="1" type="ORF">PGT21_026826</name>
        <name evidence="5" type="ORF">PGTUg99_000737</name>
        <name evidence="7" type="ORF">PGTUg99_023553</name>
        <name evidence="4" type="ORF">PGTUg99_024981</name>
        <name evidence="6" type="ORF">PGTUg99_034552</name>
    </gene>
    <organism evidence="3 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000168">
        <property type="protein sequence ID" value="KAA1072603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000474">
        <property type="protein sequence ID" value="KAA1073829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000237">
        <property type="protein sequence ID" value="KAA1121871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000142">
        <property type="protein sequence ID" value="KAA1128422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8W4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1072736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1073829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1121871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1128422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="106" mass="11610" checksum="2521772F8AFBFB09" modified="2019-11-13" version="1">MLSLVAASPRTGLSYLHVYYKGGLTENGPKAVKYNPLSYLGAHKCTVVFDSTVYCGECEHVKPPSVVERPHKTNSLMSTIQTGSKLAKCRCVFETGAWEGRERQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M940</accession>
    <name>A0A5B0M940_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi to ER traffic-protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M940"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="752" mass="83080" checksum="5FB0C2A9E876ADEF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB37</accession>
    <name>A0A5B0MB37_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dipeptidyl-peptidase III</fullName>
            <ecNumber evidence="5">3.4.14.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Dipeptidyl aminopeptidase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Dipeptidyl peptidase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_011241</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.</text>
            <dbReference type="EC" id="3.4.14.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB37"/>
    <dbReference type="OrthoDB" id="4694525at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF11">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1073413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="623" mass="70114" checksum="E1C245085EEC8968" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBP2</accession>
    <name>A0A5B0MBP2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBP2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="155" mass="15036" checksum="A0431390E4848842" modified="2019-11-13" version="1">MATRRASLTVIRIGGLTPPIWDRNCIRLLVKLATWLKGLAMGETISGPSPVVATVLSTPTVTAVPTHGLHRGYFGGPGYGGGYGGAYGSGYGGGYGGYGGGYGEGYGRGYGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGGGGLGLGVCVSSFPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MC97</accession>
    <name>A0A5B0MC97_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_004732</name>
        <name evidence="3" type="ORF">PGTUg99_031602</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MC97"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033473527" evidence="2">
        <location>
            <begin position="18"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="111" mass="12094" checksum="C4CEC64894A0A7DF" modified="2019-11-13" version="1">MMNLLLLNCVFLTNVNGYLGFDGLFSAFRLLKSGGSLREAEQGTASMKKILGEVNGMRGAMGSATRVDMKASKAGLETMRAQPLPRTFSRRSSVKTLTRRNNKHQNSAPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDC2</accession>
    <name>A0A5B0MDC2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDC2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022987841" evidence="2">
        <location>
            <begin position="24"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="640" mass="73406" checksum="6D58E84C2E39C384" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEZ7</accession>
    <name>A0A5B0MEZ7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_010409</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEZ7"/>
    <dbReference type="OrthoDB" id="4137487at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018825">
        <property type="entry name" value="DUF2427"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018827">
        <property type="entry name" value="YTP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685:SF2">
        <property type="entry name" value="PROTEIN YTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10348">
        <property type="entry name" value="DUF2427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10355">
        <property type="entry name" value="Ytp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022821830" description="Cytochrome b561 domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2427" evidence="3">
        <location>
            <begin position="56"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Protein YTP1-like C-terminal" evidence="4">
        <location>
            <begin position="168"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1074574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="27218" checksum="B72AB1EAF77EBF7A" modified="2019-11-13" version="1">MTSRWIRFKNLLFVGLAFRFLIANSKVSVVVVGHGGDHSSRPPPSPDSLPISPPKLVIVHIVLQLLTWGILLPMGVMLGLGRSRLHGPVQAVAGLLLSLPATSLSHLSARHPYPATLHAALGAPLFGLLLLQICLGFFLKSHWLQTSRFRSLIQLAHSIIGKSFIPLSWLQILLGAVASLSFCYGDHLGQCLAHMIMGSAFVGYGVVMLSLMEFGKPWLKAHQLSPEYLDCCVITVWGLVNTFTYVRSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLA0</accession>
    <name>A0A5B0MLA0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">GCV3_2</name>
        <name evidence="6" type="ORF">PGT21_010692</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="4">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GcvH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLA0"/>
    <dbReference type="OMA" id="KEHEWIR"/>
    <dbReference type="OrthoDB" id="10264154at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="57"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="3">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1077521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="163" mass="17645" checksum="E601C465C271A4BC" modified="2019-11-13" version="1">MSANLLRPVFRSCRVSIPAYSLNSLSRVSSTGFKRFVSTKRYTKEHEWVSLDPKTNVGTIGITDYAQNSLGDVVFVELPEVSATVSSGDLIGAVESVKAASDIFAPVSGTVTEVNTKLADQAGLLNKSPEDEGWLAKIQLSSPQELDNLLSEESYKAHIEGEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPV2</accession>
    <name>A0A5B0MPV2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPV2"/>
    <dbReference type="OrthoDB" id="2497876at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="145" mass="15593" checksum="F1CE225F9DD3CFFB" modified="2019-11-13" version="1">MDTDWCIVCDTKIFPHHSISCENHEDEEESGDQSTQPPVIIPFSAFCSQDCLIKSYVEAASQVQASQNPKNKTGGLSGTHSNESLMLYPGHLKKNTKSSGEGSLAKFKSNSKYISVYPGRKKVVSEFTLNSCGSSSSTASSSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRQ8</accession>
    <name>A0A5B0MRQ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019237</name>
        <name evidence="2" type="ORF">PGTUg99_002157</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000295">
        <property type="protein sequence ID" value="KAA1111238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRQ8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="198" mass="21813" checksum="F2C465B0DE817888" modified="2019-11-13" version="1">MGKFQHLLHTAPPFQSWSRHVLPLLSTYIPPLLRSIPGRDMFSHTTPRIHTTPPFHSWSRYVLPRYSSLALLSAQHTTAVKTDKNKESFDASAKFSRDLDALVEGKEYPQPVNSNSMKPLALRSLVVNGMWATPPSIALKEKQAAARVDKALIKKAVAIEKLKAKKTPKPKTLPGPAPRSRIHVVGRFAGEGSGTAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MU18</accession>
    <name>A0A5B0MU18_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017518</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MU18"/>
    <dbReference type="OrthoDB" id="10371121at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25850" checksum="CB47CCC596F389DD" modified="2019-11-13" version="1">MEDVTFTLILVAMDAVNPTILKTTIEAIPVLTEENFLAWKTQITALFKLGGLKDKIIDGEPALDDVDNTILCAIILAKLLTTTHKNVVTSTNEENAQLLWKSILKRFISNEPLNRARVYNSFASFIFEPSNIEKFITEITHSKDGEDIKPDVLIDHLEIHLNKLRVSEGSKSASIESAMFTSDDTLCSPGYHNPRSKTHIKDNCWKIYPEKKEAYLKKTQGSNMKPKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUU8</accession>
    <name>A0A5B0MUU8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020381</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUU8"/>
    <dbReference type="OrthoDB" id="10338791at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022893452" evidence="2">
        <location>
            <begin position="26"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="313" mass="35748" checksum="C44F2F43D42EF199" modified="2019-11-13" version="1">MSHHFGLRKIKLLILLFLQVHGSQCAMRNFAADRVHGVESVISGSKSSSNPIALSKSMDKPDLQDTSDLVDSNVQAKNDGSRYEEDFTGKYSEQVDHVSKILKEIEEQEPGTIIIDHKAFPIQDKSPKQVVNFPFPKKMITESNSKDIREYLASTFPFEQQSTILDSVKSIAKVQIDDQKAFDLQLEFRQKNLAELKDQIIVSLGSNNGNQNWQKLLDYTNKLDELSNTKISPEEFIKEIQEVLYKVKLESTSTSKLYSQFTLSVQDFALQIIHSKYKSNQISQNDLLKLITEDEMLKILAKTKVLTYKMKYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MV97</accession>
    <name>A0A5B0MV97_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024892</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MV97"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8741" checksum="51EA461FCF3326E5" modified="2019-11-13" version="1">MVFVLPMCSNLRGQPQVPAPGFFRWGALLEDVEVLMQQLRKPVMKSPRMIFISGLNNKLSSSHLCYSLHPTRRLNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWX6</accession>
    <name>A0A5B0MWX6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016751</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWX6"/>
    <dbReference type="OrthoDB" id="10063408at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="139"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="375" mass="43727" checksum="A73E94D02737F6F7" modified="2019-11-13" version="1">MPPFEGEGGKKRKRSRRPATSSSISLAKVLELERQHALQDLCSNPPNDRPSNHTSRGLLDESNQDVNDFESDRQHYEDHQELDNDYESDTRVQLLADYFRSEHYKRRKVLEEQHWQEVYDKMFTSFHDCATKTLHWGNQMVWDTDWKAECECTLTRKRPVVLVDLLSRRESRIEFCDCQPDQVRLIQMGYIGGAPKFPRTAFSIRLLQFHHIVWKHSAIALTPFSKALDEHLDFNSPLILVNRPELEEEGTYTTRQWRKTLSSAIDAYREMLHREEHLSEELLQMSKIDKLAELCPKCFGPTVPGKKEDEPDYIVCMDGNFQHRRHKAASNEIIPLKNPSLFIKPDDVEAMAISMSNNRIDDVEPEPSSVRTFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1G8</accession>
    <name>A0A5B0N1G8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Serine/threonine-protein kinase tel1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TEL1_5</name>
        <name evidence="1" type="ORF">PGT21_001926</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1G8"/>
    <dbReference type="OrthoDB" id="2504434at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="355" mass="39074" checksum="32981C68E1F86B2E" modified="2019-11-13" version="1">MNSESQASPQAEDLVRKITHVISNDVELYTKSSSISLGTLRLVVPRKGFDSSFFCTKGFTRTSCDGVDVAIERVQAVTWAALRLGASALNHVTQSTHQPSARGPETPSKRRKVESELEIFLQDIQPGSLSAAVVHRIQLLTVTVDLHWENLDNSSRQKIIERVIGLLQSDDPVVVSWAFIFFGNTAHCIAPPGNSSSSYQETPPSDSWGPNIEHWKQIWSYAIHRTANPTCSRSASYAAFCILVTHQLPVHATHEAIHGFLRDLDVQGPPFPYDSVCMLISECLALASEDIQLSAYALEENVLRWLNKTWPEVDRSTATCNLIGGGSAQRNRRALTAGFDSEAVFPITGFDSKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N295</accession>
    <name>A0A5B0N295_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N295"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="182" mass="19479" checksum="CB6FBFE457B8983D" modified="2019-11-13" version="1">MVADNPLRISVIRWRIPARANGYPPAGADSGADVPVAAAVGCVAQPTAAAPLGCWTLGYTHVLLGGRSTAHLFLILVGGRYTALRPSLSKPTTPTAARKKPQPRTGFLIFGDTERFSRPIVVDRGLPSSLKVFLGSGPRRLGPIASAHPFPFPHRRRSSLRSSLRRRFPPLLPTPTPPGPWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5E5</accession>
    <name>A0A5B0N5E5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Kinetochore-associated Ndc80 complex subunit ndc80</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NDC80_2</name>
        <name evidence="4" type="synonym">NDC80_3</name>
        <name evidence="3" type="synonym">NDC80_9</name>
        <name evidence="2" type="ORF">PGT21_005201</name>
        <name evidence="3" type="ORF">PGT21_016119</name>
        <name evidence="4" type="ORF">PGTUg99_000788</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000209">
        <property type="protein sequence ID" value="KAA1123305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5E5"/>
    <dbReference type="OrthoDB" id="2508320at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1123305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="54" mass="5893" checksum="33F2B6D8B0CDC26A" modified="2019-11-13" version="1">MNSILGFIVSYKEQIAEKTLQLKHLSEPTGSPSINLDVNYPPSGSGSNNTNMRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6W0</accession>
    <name>A0A5B0N6W0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CHS3_1</name>
        <name evidence="14" type="synonym">CHS3_8</name>
        <name evidence="13" type="ORF">PGT21_000446</name>
        <name evidence="14" type="ORF">PGTUg99_031886</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000109">
        <property type="protein sequence ID" value="KAA1085005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000003">
        <property type="protein sequence ID" value="KAA1138339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054295">
        <property type="entry name" value="CHS4-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF16">
        <property type="entry name" value="CHITIN SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22997">
        <property type="entry name" value="CHS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="570"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1155"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1183"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1210"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase 4-like" evidence="12">
        <location>
            <begin position="477"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1392"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1417"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1459"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1491"/>
            <end position="1500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1085005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1138339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1520" mass="167320" checksum="963706D76ED364D4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9R8</accession>
    <name>A0A5B0N9R8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030443</name>
        <name evidence="4" type="ORF">PGTUg99_023554</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000342">
        <property type="protein sequence ID" value="KAA1099229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N9R8"/>
    <dbReference type="OrthoDB" id="2505311at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="220"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1099229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="455" mass="51039" checksum="E876557F5A103E0E" modified="2019-11-13" version="1">MTSTAPSDYEPESNRSADSNASKAIKTEEPETKLPAVSHELETKPSAETNEGELNASAEENGEPPAKRKRNMTETRKAYMRSSKNKARQKRRKTSRDNEFETLISDQTMPGGVLIRGKRVLKFDLFPEISKELAIEKEKRMKEEQEFKENSQNRPDEKIQKPAQHPLTPRAPTEEENATALKLVEQKFHHANRDYGKIYNSANEQIIGVFEILKIPNLEQTQREDLDFLCAFIHDCKQFVSPAISEGLSCDDISSAIGWSKDLTRLEILPRYRDEESIRKNQEAYSKLMEASKKAGGILWDIFSTLGNLAAENTRAKMKKLASDGLVSSLAFPSNGFHTDYQVENEEESDPPLSFAMMIPTFRSTGKIALESEGHRVDNGQFVFPDIKQAISFPPDTIGLMIFRAQQYAHGTLSSTEVGDSTRLGLSIQAPIVVGKSSITKQDTPHDSEPKKVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NB97</accession>
    <name>A0A5B0NB97_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_030502</name>
        <name evidence="10" type="ORF">PGTUg99_008429</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000414">
        <property type="protein sequence ID" value="KAA1086046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NB97"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09326">
        <property type="entry name" value="LIM_CRP_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000145">
        <property type="entry name" value="Cysteine and glycine-rich protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000001">
        <property type="entry name" value="Cysteine and glycine-rich protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF35">
        <property type="entry name" value="MUSCLE LIM PROTEIN MLP84B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="26"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="260"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="120"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1086046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1104720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="333" mass="36515" checksum="834F66E6FC87162E" modified="2019-11-13" version="1">MSRTNTIHLNHRPSTNIKRAFAPATPKCPACLKSVYAAEQALGPGATPYHKSCLRCTHCKKVLDPFSILEHGYDPYCKICHSKAFGTKGVGFGNAVVGEYTPRSPSPKLRSPVSPTIAFGSSLREERDSPSKPDLPFEPVPDHHQPIHAELKPTDDDQADEEDFEPPSLSSLQIIVKPRPKTSETPEEVLHEDRTTVSRSSSTLHSTRQLSLKQEHPLTPPPKPNTSALSFPARAASSSPYNLRPTPTFPRPGFGADGPQRCPACTQTVYHAEQVLAIGKKWHKRCLRCTVCSKALDSNFNERAGKPYCTKCYDVHFGTGAHGFVLRAGFAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIN3</accession>
    <name>A0A5B0NIN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008707</name>
        <name evidence="2" type="ORF">PGTUg99_030757</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIN3"/>
    <dbReference type="OrthoDB" id="2496225at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="616" mass="70242" checksum="700EFD8B040F596F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKR0</accession>
    <name>A0A5B0NKR0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcription activator of gluconeogenesis ERT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ERT1_2</name>
        <name evidence="15" type="ORF">PGT21_006770</name>
        <name evidence="16" type="ORF">PGTUg99_002094</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="16 18">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERT1/acuK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000027">
        <property type="protein sequence ID" value="KAA1136757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKR0"/>
    <dbReference type="OMA" id="EDLIYME"/>
    <dbReference type="OrthoDB" id="2538135at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050335">
        <property type="entry name" value="ERT1_acuK_gluconeogen_tf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659:SF1">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR OF GLUCONEOGENESIS ERT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="13">
        <location>
            <begin position="88"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="14">
        <location>
            <begin position="294"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="363"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1089094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1136757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="507" mass="56073" checksum="B7C46185E11FD3EA" modified="2019-11-13" version="1">MVAEVSTAAIQPHQYHHLNPSLLSTNRLLPQILTNTGRSSCASSSSSSSCGSSPATTHTSADSWNLKPQTITCKPNPPPARRKKASRACGHCQKAHLTCDDGRPCLRCTKRGLSKTCTDGVRKKAKYLSDYDDALLVRPRPSIKRASEDPLEVEDCKKVKVATDDRDCRLSISWADLDLLENVGIPQYPNVETSVLAGCETVGSANPTSQTWALGGLEEWLARFESGVSPSSSAALDNLISLQENVKQADQTSESRQRMQEWVTRINRLVEIRKLTMNITADDEWMLKRNLDRSLMELERIISLSGTPTMIWNTSGQILKVGDEFCMLTEWSREDLVGKHIETVFEPESLEEYLDMVESSIFPDQESNHHPSRPRSQGSKTSSRNLPQRSSSSASSSSMTSSPSLSSIPLIALSQASDSNLSRGPYSNDRPDLTSSFAQSKTSNQSYGERECKETIKWFGIRKACGRESIGCSFCFKVRKDLFDCPSTIIGNFLPILTRASYTDHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKU8</accession>
    <name>A0A5B0NKU8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_010673</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKU8"/>
    <dbReference type="OrthoDB" id="1939598at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14705">
        <property type="entry name" value="bZIP_Zip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR ATF 4/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044:SF14">
        <property type="entry name" value="CRYPTOCEPHAL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="222"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="58"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="182"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="295"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="58"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="332"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1089222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="348" mass="38685" checksum="99D0A8F67C3B099E" modified="2019-11-13" version="1">MADDWPFLHLNIISPPIHRMGASDEQELSLLPFDSSHLEAWSNINFQFDEPPLVRFDNNNQANDNLNLNPNSNSSTRDTRLTSSSTTGSDKNFNNNPFQLPNQHGIPPFLDNPSGLNNPHELTYLSQPSNQAILSTSDQPTDNLTSNNTNNQTTIEPTSIEAPHAKAAVTSTAAKSLLAHDQNHSLHKQDNSSPPNNSDGNSNNNNQSGNDDDDANRVAYDEDKRRRNTLASARFRMKKKMKEQEIERTAKEMRERVSELEKQVDSLKQENKWLRGLIVDSTASKLVSAQVQETAELSQLESTSGKRPRPTLSPTTDSIHVPAHYQKRPRQSSSSTSATTTSKSSATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NP58</accession>
    <name>A0A5B0NP58_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_021904</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NP58"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="52"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="702"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="753"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="624"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="761"/>
            <end position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1091055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="820" mass="90697" checksum="66BB6D993BBAB834" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUL2</accession>
    <name>A0A5B0NUL2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUL2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="56" mass="6129" checksum="060D101A665DA9A6" modified="2019-11-13" version="1">MDGGSARRSHGKYDLLMENLSNGASRSLNILQPHSDHVPDDMSNSSGFQTNVLQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUS5</accession>
    <name>A0A5B0NUS5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012170</name>
        <name evidence="3" type="ORF">PGTUg99_031076</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUS5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="102" mass="11181" checksum="022CF21781D18BC3" modified="2019-11-13" version="1">MKASSSIYTSPSTSSRAYSDAANEADGMKPMRIVSTCNRFTTGVIDKEEDFVSITSPARALYEATRNPFPSHSTANARKENDKDRDPTGVTRFPFNGLQLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVZ5</accession>
    <name>A0A5B0NVZ5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase II assembly factor Rtp1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_011727</name>
        <name evidence="6" type="ORF">PGTUg99_030308</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Tango6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVZ5"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019451">
        <property type="entry name" value="Rtp1_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039600">
        <property type="entry name" value="TANGO6/Rtp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959:SF1">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10363">
        <property type="entry name" value="RTP1_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="4">
        <location>
            <begin position="814"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="516"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="643"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1008"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="654"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1026"/>
            <end position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1092704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1093805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1175" mass="130164" checksum="F8037427241000CE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P345</accession>
    <name>A0A5B0P345_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P345"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="114" mass="12107" checksum="93E142EF99104C57" modified="2019-11-13" version="1">MCPSESLPMCLGVNSWSISHVAAQPASPTGPRTLEPPADAAPGGGGMECTSNSTDGSLPHTLSRRADPSSISKPQTSSNGLRPTGKRAKRDHKLDCSWSRVTLTLQHHQHLRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P554</accession>
    <name>A0A5B0P554_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024577</name>
        <name evidence="3" type="ORF">PGTUg99_020674</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P554"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137662" evidence="1">
        <location>
            <begin position="19"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="117" mass="12390" checksum="B5C61EB3CF9FDF2A" modified="2019-11-13" version="1">MKIAMFIAALSVASGVFAAPSQAEANAGAAKDAKNDAKAFYPGYPAPGPFYPGAAYPSPYNPPGPYYYGGYPPTYYTYQYPAYTYQYTYPIPPTQGSTGPIAASTVPLKTPNAPPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P981</accession>
    <name>A0A5B0P981_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P981"/>
    <dbReference type="OrthoDB" id="2515550at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="774" mass="90051" checksum="65C53B4877838F2E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9S3</accession>
    <name>A0A5B0P9S3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019462</name>
        <name evidence="3" type="ORF">PGTUg99_008636</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000173">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9S3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="118" mass="12788" checksum="428C9EAE42325779" modified="2019-11-13" version="1">MLFGQSKTFSTITTRNRLLTIVSTADGAIVDGDGGSESQYKPWPLVPTREMSGDEANNLPASKKPKLDLQLSLGLSSKDAESVKPGPSSHQSRRSHPNGKPLTLQQYLFQMGPIMCLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PE85</accession>
    <name>A0A5B0PE85_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032216</name>
        <name evidence="2" type="ORF">PGTUg99_013233</name>
        <name evidence="4" type="ORF">PGTUg99_018024</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1098990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000072">
        <property type="protein sequence ID" value="KAA1133270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PE85"/>
    <dbReference type="OrthoDB" id="10274478at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1133270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="119" mass="13283" checksum="5EDDEAD67A3479F1" modified="2019-11-13" version="1">MVQPDARSDKTQNSRQPRPREAYLDDRARIYYQASHPALVGQLHPGAPPVRHPRTLTIIMREARPLSRDLGTPATPAPGTQGVLRSPSSDKKVDKDERTQMRAVAFSRVDSLLSRRSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFC4</accession>
    <name>A0A5B0PFC4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycoside hydrolase 131 catalytic N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFC4"/>
    <dbReference type="OrthoDB" id="5283326at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041524">
        <property type="entry name" value="GH131_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612">
        <property type="entry name" value="GH131_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612:SF2">
        <property type="entry name" value="GLYCOSIDE HYDROLASE 131 CATALYTIC N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18271">
        <property type="entry name" value="GH131_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022902721" description="Glycoside hydrolase 131 catalytic N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 131 catalytic N-terminal" evidence="2">
        <location>
            <begin position="33"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="232" mass="26039" checksum="B8A161DD82045199" modified="2019-11-13" version="1">MSQRLSSASSASLFLLVVLSALVAPSLSKILLDYRIPPTAKVTDMGDKTTALGKLTKATITGKNSLTEAYQFMKVPKSQFQVVALKINDNSIFKDQLGFRRNDIVPEYNAKLFETGTKTYHHSFIFTKGGKLDLKHGYLLASVEFPDAVSTHMFDIFHGTQFDSKNTAGTADKDANKIRVRDINFKTLFDVDVKSDVIFNFAIEVDWKAKYVFSKKSAIILRRRKALKFILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGU2</accession>
    <name>A0A5B0PGU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP synthase subunit alpha, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ATP5A1</name>
        <name evidence="1" type="ORF">PGT21_023024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGU2"/>
    <dbReference type="OrthoDB" id="10307835at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="167" mass="18567" checksum="3454E446930FA6FA" modified="2019-11-13" version="1">MLNVTMDMYLADVLESLVEKKSLGRPRALLPLHPCPSTRHCCPPRPHRLVLAMRQLPHPRPSILIKAASSSLGLFLYCFVSSTIHPPACLSTKINVFIPLLTLIGLFESDFATSASILPVPESITLIGFHHQHQSDFTCLTSPYHIILPHLLPPPSTLSDSRLWHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGW1</accession>
    <name>A0A5B0PGW1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_036683</name>
        <name evidence="13" type="ORF">PGTUg99_028140</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGW1"/>
    <dbReference type="OrthoDB" id="10261467at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000965">
        <property type="entry name" value="Gll0198 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286:SF2">
        <property type="entry name" value="BACTERIORHODOPSIN _OPSIN, NOPA (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01036">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00251">
        <property type="entry name" value="BACTRLOPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0157">Chromophore</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1098587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1100431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="242" mass="27240" checksum="C497C2EB6D44500B" modified="2019-11-13" version="1">MSSVPTTKILWVTFGLMTISTAAQWVLTTRQQPNTKLLHHISLTLSTLTLVTYYFILTDINVTGSSIWIRDLHWILTLPLVLVELGLISGVSIQDLVIMALNGIGMGVTGFLGTIQRSNRLRWPLVLMAWIFWLSIVREILTNGRRSSARNSVRVARFFNYLAAYIVLLVSFYPIIWSAPVDPFLEIALFSILDMLTRPIFATWILIGVSIIPESNVVGANRYIEARIWNSIGGNIEEPPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHU8</accession>
    <name>A0A5B0PHU8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030750</name>
        <name evidence="3" type="ORF">PGTUg99_019992</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHU8"/>
    <dbReference type="OrthoDB" id="498286at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022036">
        <property type="entry name" value="DUF3605"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020:SF2">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12239">
        <property type="entry name" value="DUF3605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137760" evidence="1">
        <location>
            <begin position="21"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="287" mass="32720" checksum="A1BD26FA62E255AD" modified="2019-11-13" version="1">MDFKLTCGLIMVLSSAVTHSTIPGVVPVGGGIFETGNAEMSRDVLYPCQGDAHTWDSVIGAIVQGGGSLIKRSPEGEKAMQAARDKAPGPAKSGEYIKEMLGWQSELPAESEAGNFWEEKVYRDRRRVALIKNEFPYGLPPLVDHWMLWIRIQTFTEAAFEPLSHETLPNPDFKRSIRRKALLRYLNYYDFYGYSGISEDVINRFRLSGFYHPTEDTVWKDPESSEVVTRLEGIEAMHWLGRHVLNVIHVIFSPERYEILFNRSTERWKSVVYPDHFHVIVKPKSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PI06</accession>
    <name>A0A5B0PI06_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_031691</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PI06"/>
    <dbReference type="OrthoDB" id="10259820at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.210:FF:000001">
        <property type="entry name" value="60S ribosomal protein L22 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002671">
        <property type="entry name" value="Ribosomal_eL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038526">
        <property type="entry name" value="Ribosomal_eL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064:SF31">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL22A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01776">
        <property type="entry name" value="Ribosomal_L22e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1100210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15863" checksum="48CBE23812FC6F14" modified="2019-11-13" version="1">MTFSPPHFRDRYTLDSIYCQSKTIVTQKFFVDFKKPASDGVFDGPAFEKFLHDRIKVEGRAGQLGDKIKIQSEGVHKLSVSSTIPFSKRYLKYLTKKFLKKHQIRNYIRVIASSKNTYELRYFLVDEPDAEEDDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJS9</accession>
    <name>A0A5B0PJS9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJS9"/>
    <dbReference type="OrthoDB" id="10425699at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="426" mass="45950" checksum="D9162095FE1C3F27" modified="2019-11-13" version="1">MPEFPARREHSACPKQTGQSFGQGALPPPHPPTLKILSPGSSSPQLPSHSPVFPPCPPAPTVKASSIRPDRSRRRTRANSPNSHGRPRPPKRPRTATRGNSLDNFVVPDSASVSAASPTPSSRPPSPPPQPPQSSPESPAGSDRMTPRPHESDFSSRPEYRAALRSWASRHHDIGLSRSSFGTGCRPPDGARQPTPERARSPTPVRSPAPANTTIDIEPIVISSGPPTPMDRSPVHRSPPDNPMRIDPELEDLRPTPPPSVGDAPERTTPDSSPPPRLNFLHTPRPAERGPSEVPGSGHSRAITPRPVVNNPPFAPTRTNPFPASRHFRHDSALRELFPDEDESAFDAIPATVVEAVIEDIQGRFETILSASVLPPALRRSTYAAFLSDLRDLRRHRLQVMAVFNPRSANYPDGNGDDPAPGSGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKE3</accession>
    <name>A0A5B0PKE3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Delta(14)-sterol reductase ERG24</fullName>
            <ecNumber evidence="3">1.3.1.70</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">C-14 sterol reductase ERG24</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Sterol C14-reductase ERG24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ERG24_1</name>
        <name evidence="21" type="ORF">PGT21_027553</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP(+) = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:18364"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.70"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:18563"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="16">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.70" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKE3"/>
    <dbReference type="OrthoDB" id="10262235at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050613">
        <property type="term" value="F:Delta14-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000009">
        <property type="entry name" value="C-14 sterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF52">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE TM7SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01017">
        <property type="entry name" value="STEROL_REDUCT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="13" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="370"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00083315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1101681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="431" mass="49689" checksum="6920E07889540BD6" modified="2019-11-13" version="1">MGQATETTKRTVVNPRTTEFEFGGRIGALGVTLSVPFFTYWLNLACTPQTGCLLGPKILDLRTLWNTTSFFSLEACYVYLGWYMYLVLCWLVLPGKSVDGTVLRDGTRLSYKINAFNSLICTILLTAGVFARWGDSPFLYIIDHYVELITASLIMSISQAVYCYWSSFQPGRILALGGNSGNVIYDWFIGRELNPRIASFDIKTFNEMRPGLILWALLDFCWVIKQVHEFGKPSNSILLAFIFQFWYVFDAEYNEDIILTQMDITTDGFGFMLSVGDLTWVPFTYSLQLVYLSFVPLHLSNFELALVLAVQLVGYGIFRISNEEKNAFRRRSENPKGLKFMETERGTRLLISGWWGVSRHPNYLGDWIMAWAWCLPTGISTPITYFYAIYFAILLVHRQMRDEHACEKKYGKADWNKYKKLVPWKIIPYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PN96</accession>
    <name>A0A5B0PN96_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PN96"/>
    <dbReference type="OrthoDB" id="10421623at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="87" mass="9660" checksum="5B979AAFD09CF8AA" modified="2019-11-13" version="1">MVTLVLFVVSNFPLSDPSGTLIRYPTPLLSSAISFKKSDFHLQSLELDSKGSAFRSTFDLVDKVLKGTKSNKSKTHQCRGHCPLVQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRS4</accession>
    <name>A0A5B0PRS4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent DNA helicase sgs1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012336</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRS4"/>
    <dbReference type="OrthoDB" id="10530333at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9664" checksum="ADF4D60DE2CFC72D" modified="2019-11-13" version="1">MRKGFAKCKCSNCEPDEAGQLLEVIQQMNIDNFDTILDDPHGFTKDPNIVTLARKRKKAMGKGSCRLPQDVASNLVAHLLHCFEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSU9</accession>
    <name>A0A5B0PSU9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_004229</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSU9"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000655">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF479">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022989445" evidence="6">
        <location>
            <begin position="26"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1103019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="488" mass="53404" checksum="AF13398219FED001" modified="2019-11-13" version="1">MDYSTQTKLSFFFLLLSVFLVELGAVTPPPTNRKPSTASPPNAASSVNFKSPLARQFVVPPRLPGVPFPIQQSYAGRLNVSVNPRQVSQMFFWLVPADDAGGSKDLTIWVNGDACSGLVGAFQGNGPITMNPGRVPTKNPNSWTRSSNMLYLDIPLNIGFTRGQATQRGSADIGGLVFNFLTAFLKVFPEMNGLNLYMAGHAYSGTYISYAADKIYAMQSRLALKLQGILLINAFISTPDYQQHIPIASYVTKNNDAFKFTPAQLKHLQATDNECGFSQYMAQHLTFPPRGVFPQVTKAVDPAHAPDFKEQCMIFMEMFQGSSPDMNLGNIHDKPGMADPNFTKFVYPGRADVQSAFNVDGARRWASCGDFNKVFPNGDASPPPTLKVLPNVINRSKRTVIAHGLLDGTLPVDGMKLAIQSMTWGGKQGFQQSVNSKFMVGGRYGGRYHTERGLTFVEVAQSGSFMPHDQGQAALSIFEYLLGKRPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXD3</accession>
    <name>A0A5B0PXD3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXD3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="90" mass="9772" checksum="5ED6B708F9BF210C" modified="2019-11-13" version="1">MRSSQSGPTDEDARPPAWGEGLHLFSSDGDGCWASALQQQTGHHFEIGNAHHSRVRANTNPWRGLSFWHATSEAPLIPQLGDLIVAAESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXP8</accession>
    <name>A0A5B0PXP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXP8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022764236" evidence="1">
        <location>
            <begin position="25"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="124" mass="14569" checksum="25CA3FF69A92A0FD" modified="2019-11-13" version="1">MVYFTLVGWLHLITLGLHINSTNSNLKFRQPTRFRINPQDSSIFSFQDQYSRILKVPQSSRFIQAQLSLPLDHLIFLVLRGLWSFVELTRPPYRSSSVKTLDRVFKRSIERSNADRVFKRSIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYB7</accession>
    <name>A0A5B0PYB7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024130</name>
        <name evidence="2" type="ORF">PGTUg99_026241</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1121008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYB7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1121008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="66" mass="7689" checksum="04F655EEEB0878D2" modified="2019-11-13" version="1">MKISDEGVRQIIEDFSILEDESFAARFLANQGDEWRLKWLQEHAKLKQAELDEKAKLKGKAVLEIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYZ8</accession>
    <name>A0A5B0PYZ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYZ8"/>
    <dbReference type="OrthoDB" id="10337855at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="709" mass="82578" checksum="BCDBFF464B3E7852" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q244</accession>
    <name>A0A5B0Q244_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006844</name>
        <name evidence="3" type="ORF">PGTUg99_003067</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000085">
        <property type="protein sequence ID" value="KAA1132283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q244"/>
    <dbReference type="OrthoDB" id="2505617at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="132" mass="13973" checksum="7B265D787627E6BF" modified="2019-11-13" version="1">MDFKSPNSSPEGTVSEIRAKRPKPVLTLSYHLPTGVVQGRLTAGGNTFSRGARSPALVPIRSPVEPPAPRSPFGPSTAILKAPHLSQSKESFSPLPPTPTTAPADAPRASVFEAFQAQFLQSQSSNLAQEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q287</accession>
    <name>A0A5B0Q287_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">DBP3_3</name>
        <name evidence="16" type="ORF">PGT21_008450</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q287"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="324"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="536"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="712"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="213"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1107265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="749" mass="82877" checksum="6E138EA6D50F6423" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2D0</accession>
    <name>A0A5B0Q2D0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007311</name>
        <name evidence="3" type="ORF">PGTUg99_037085</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2D0"/>
    <dbReference type="OrthoDB" id="2500825at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="290" mass="32089" checksum="37D7621671BE2C90" modified="2019-11-13" version="1">MFLLPCCLGRSRKLETSKVEQPADNDISSCSDSEVVSAMKEIEPKLPQAAPTPRRTRTTDSNKSGMRSRSSILSWCSVTSVINFCHTLKSSEPSPSMQASPSTIPCTSPIPAANPRTKLNDISSILSLIIQEEGEDQLPNRRQSYSDDPFAPPTPRATRFGSFSNCSLDDHTTLRSSVQSTPAEANNLNISNIEDRTLTLPESVCLPNIDRLSKFDFNEWNPKWIKNSPVIQPIPVGHLSMSTIRMSPSPTLTYRPQQFARRGSRTPTLSSHTNYSSRTEDSNQIPPPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q496</accession>
    <name>A0A5B0Q496_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031928</name>
        <name evidence="2" type="ORF">PGTUg99_011104</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q496"/>
    <dbReference type="OrthoDB" id="16464at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF11">
        <property type="entry name" value="LD23561P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="48383" checksum="320D7ED8DF91DB8D" modified="2019-11-13" version="1">MSPSTSSTKPSVLILGGLQDGHARALLAFLVPSIADPAQQSARHIRLVDKYLCLPQDDAYTTYMDSECQKILKAGESIGVEYLQANLLVASSRSKAFTLPEQYRTKTGGGGSETYDYVFDFTGETDYNAEEGVHVERTGRLLPELGRVAAAMGVKAYIRELPALYHSDNKKPLKEGEGKPFSMRSKWVHEGVRAMAALPDLNIVVARPALLYGPYAVDGYAPRLLIGEIYKYIGERMEHLWSSDLRLHTIHTHDFAAGLWALAGWMSGLGRAAAEAHANPIPCLLPNAEELPAGMPARDQVVKAPFFNLVDDGDTTQGLMASLAEKVVGVKVGFYNKVVCQFAKLNMIDVIEDVNEKHCEPWPEMLSKSTPPITNTPFTPNLPRALLTKNHIAFDGTKVKEVVGFKLKFPSMTEEAIRDQIQKFIQDGVWPNAPPKKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5G4</accession>
    <name>A0A5B0Q5G4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocation protein SEC62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SEC62_2</name>
        <name evidence="12" type="ORF">PGT21_007639</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC62 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5G4"/>
    <dbReference type="OrthoDB" id="200187at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004728">
        <property type="entry name" value="Sec62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011553">
        <property type="entry name" value="Sec62_asco"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00869">
        <property type="entry name" value="sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443:SF9">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03839">
        <property type="entry name" value="Sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1108307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="239" mass="26924" checksum="33C5A8068983ED77" modified="2019-11-13" version="1">MEQQKAIPRELLAVVQYLRSKAELKTKTGVLEGKRHDYFKGKAAIKALLSPSYAKIHPSSSPPITDEAAATALLNTIIPYAFFLRVERTTQSIAGAKVLQINPTQFFDGESYYAWLYDGNQLMVIAGAALMVLVVLAGVMFPLWPMSLRIGVWYLSMAALGFVGLFFVIAIIRLILYIITWIAVSPGIWLFPNLFEDVGFVDSFIPLWSWDIPPPKKVRPDHSYLHPKSKTYLNKRMIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q651</accession>
    <name>A0A5B0Q651_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q651"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="54" mass="5956" checksum="6AA8F7DB09BA95FD" modified="2019-11-13" version="1">MHIGRPRSVSGRESSRLLHKRPIAFQKSVERSVNTARSRSAGAVSVAKEIVELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6M5</accession>
    <name>A0A5B0Q6M5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6M5"/>
    <dbReference type="OrthoDB" id="3780939at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="189"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="483" mass="55899" checksum="C4A030F871C838AF" modified="2019-11-13" version="1">MNTASSFGDQYQEIVIQMVDNGLSESEILKSLEENYSVKISRRTLSRRKEDWGLTNHAAQQIHSLEETITKHFEDGLTYAQIHHAISTSHNYTHSERTMVRQLNSMQLSRRLDDLDRNLTTADTAVSCIMALHQTPEGRNAGYRKMRQLLQTQYGINLHNMTVAVINRTLDPTGVDNRAKRVLKRRVFNTPGPNFIWSADGHDKLKKFGLTIYGFIDAWSRKILGIYVHITNNDPRHIGYYYLQLVKQMGGIPRRTTTDRGAETIHMAGHQINLTEQYNIESTDSSQAHLFTKSKHNQKIECLWSQLMKQFNTELINKLFQALEEEYYSQEDPLQQLLFLYLWVPLLQESLDRWTQNYNSYKRRLDKKTMLPTRCSANWCYNYPEEQGGEQGLIPVPSEAVEALQAAFYPDGVELMRTSPQWFSESIGEILHGMQLEIPVVDVHNVWIVFELILEAIRKFDSAWLSDPTNDPSMTIEAQAQEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB71</accession>
    <name>A0A5B0QB71_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QB71"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="53" mass="6011" checksum="CC7779F92228E1FD" modified="2019-11-13" version="1">MSISVFFIDCVSMITLIGQTGQMFISTQGAAQLLRPYHDLDINFDTKYSQNCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCC4</accession>
    <name>A0A5B0QCC4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Kinesin-like protein kif21b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">KIF21B_6</name>
        <name evidence="2" type="ORF">PGT21_025102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCC4"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050295">
        <property type="term" value="F:steryl-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904462">
        <property type="term" value="P:ergosteryl 3-beta-D-glucoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF6">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 5 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="324" mass="36180" checksum="4B759384DFA52941" modified="2019-11-13" version="1">MARGEKGDFAYFMQVHPAAPYPASHYHSLPIGSQTTPTDRKLNQSKKKSGRASEYLSASPPLLSQWPHRPNGISYIGQNLNLQDGSADVHLTRLHPWGFNCLRFVTVWEALEHQGPGQYDDNYMEYVVAMLRKCKEYGFLDKHVVQDGDQLSGHGGHAHWRWSKRDQETDGDDEARVEQDQGAQGDLSKGASSHSSTPALLNDDDDSLCPAVYLAEQVHKFFLLNQNSQVRLHGPFNWPIKIDSQLVNILKSHFAIRLPSVVGASSGLSKQPTSSAVMIHWNSQIVSSYSNSLTHSIASSTSTSHFKMYTGFRAKFKIFISLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCJ9</accession>
    <name>A0A5B0QCJ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">21-0</strain>
        </source>
    </reference>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="66" mass="7027" checksum="5ECF3CE5341DAD71" modified="2019-11-13" version="1">MLYGLPDWNLPQVPSLYLSAAASRGQEAKSCQPVRTRGQVTIPCPAFGAGPRLCAKGWVESVIVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCT9</accession>
    <name>A0A5B0QCT9_PUCGR</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034953</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
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            <begin position="496"/>
            <end position="516"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
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            <begin position="528"/>
            <end position="548"/>
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    </feature>
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        <location>
            <begin position="584"/>
            <end position="609"/>
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    </feature>
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        </location>
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            <begin position="85"/>
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            <begin position="150"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="206"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    </feature>
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            <begin position="822"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="832" mass="93138" checksum="2AB0EBC02BDF1FB0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QD09</accession>
    <name>A0A5B0QD09_PUCGR</name>
    <protein>
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            <fullName evidence="5">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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            <begin position="1"/>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="46305" checksum="E29B40B4EBF42D58" modified="2019-11-13" version="1">MGNNLEQGVSQSKDDARATNTIGSSSTTPELHGQQKIQNIKTGDSNKKKRSPNWLPQEEEQLACSWLVVSQRPEFITNQTSESFFRAVELDFNQHSKLHHRDHDQIRIRWAALNTSTLKFSAIYNALEKSPPKDSTPEDWMATARKIYSEQSRGHTFKDELAWKKLRHAPKWKQDNKKLNYHQVLMPDLSVDVHQQHQHQRLQAIGQPEQTLNQHHQQLHQLLQQPQDQLLPPPQQQQQQQQQQHQQVECSSTSTQHHSPRSDSPLTQTIEGSRSKRLRLDQDLEHLIAQTTGHDSSHSIPVIPTDPHEKRQPEPSNENNEDQDQLDLNTFDSLDDQGQQRLVAQNLQLERELKRSQIEMNNLKLLMQSEADCPDQESVILLRLMKSRLKKRLLLDQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QE84</accession>
    <name>A0A5B0QE84_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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            <begin position="18"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="85" mass="9973" checksum="A68A67A668CBA636" modified="2019-11-13" version="1">MKSSRSSAKAPIRWKRWRALSGLRSPELRPRRAAFRRSHSPAPTPTPNARKQKKEFPRKLNVQQRTYNPPPAEIEQTRTIITRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEA5</accession>
    <name>A0A5B0QEA5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FGAR-AT PurM N-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002881</name>
    </gene>
    <organism evidence="2 3">
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1111518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="6666987at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055181">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22689">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGAR-AT PurM N-terminal-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="115" mass="12201" checksum="9A6F95C8E6F43632" modified="2019-11-13" version="1">MVGPYQVPVWDVAVTRTSYGFDLVSGEAIVMGKRSPLSLISAASSAKMVVAEVLTNLVAADINSLEHVKLSAHWMCSASHGNESAWLFEVVGIELCAELGISIPVGKDSILQPTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEM8</accession>
    <name>A0A5B0QEM8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEM8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="105" mass="11887" checksum="E7B0D37721FC2669" modified="2019-11-13" version="1">MKRTAPDHEVIDIDEIDSEQQDSQSGANKRSFAWTHFKDKPGTMEAVCQVVTKSGAICGRSIERTRAQAQNHFTPTCQASIEYPTHISPRKPNGPPERNRFQDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEW1</accession>
    <name>A0A5B0QEW1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001677</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEW1"/>
    <dbReference type="OrthoDB" id="2499294at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023098178" evidence="2">
        <location>
            <begin position="25"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1152"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1111493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1242" mass="137801" checksum="02D99AC87AD26BD8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFD6</accession>
    <name>A0A5B0QFD6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein G</fullName>
            <shortName evidence="9">snRNP-G</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_016318</name>
        <name evidence="11" type="ORF">PGT21_018294</name>
        <name evidence="12" type="ORF">PGTUg99_011286</name>
        <name evidence="14" type="ORF">PGTUg99_034441</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="12 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000136">
        <property type="protein sequence ID" value="KAA1129687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFD6"/>
    <dbReference type="OrthoDB" id="2146at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01719">
        <property type="entry name" value="Sm_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000023">
        <property type="entry name" value="Small nuclear ribonucleoprotein G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034098">
        <property type="entry name" value="Sm_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF2">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037188">
        <property type="entry name" value="U6_snRNA_Lsm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1067832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1093419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1111930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1129687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="78" mass="8780" checksum="1266A6E3B2D29EAD" modified="2019-11-13" version="1">MSKAAQPELKKYMDKRLFLNLQGNRKLSGVLRGFDIFLNLVLDEAQEESLEGGEKRKMGLIVIRGNSVSSIETLEAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFE5</accession>
    <name>A0A5B0QFE5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_015197</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFE5"/>
    <dbReference type="OrthoDB" id="1894652at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21397">
        <property type="entry name" value="CHROMATIN COMPLEXES SUBUNIT BAP18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21397:SF4">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21203">
        <property type="entry name" value="ECM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022922985" description="ER membrane protein complex subunit 10" evidence="10">
        <location>
            <begin position="37"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="182"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="235"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1111908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="369" mass="40243" checksum="9DF0E454DB93A609" modified="2019-11-13" version="1">MMTAILDSHRRKSEGQRLRLCLVLLGILIISPVSNQASSGSSYALFHRLVQSSIAHTPSSTTATLGGGEWQRRGLLEIVPDDGSLDHFKIASFSNQLVDPLVLPKETLISSGKGWSYQIALAPLLAFNLPGQDPSPDTGFALVSFPLCRFLQDPTSSSQSNGLSSPATRLEEQMTIWLRPRRPIGLANNHPNPSAAAAEEEEEEEEEEEEEESWRRSGERDLHLDLLGFQWTSSHELSPASSSPTCQDTHHARSSSSSESSINAESPTGSASLDPFLASLSHPQNQSSTLKISLRVPERLSEPIWNPHPTVQPAQLMADGSLKAPAPEKGWLAKYWMYILPVVVMLVVGGGPPPPEEEGGDSTNQRHRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFI7</accession>
    <name>A0A5B0QFI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFI7"/>
    <dbReference type="OrthoDB" id="2507436at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="305" mass="34428" checksum="9CC8085DC5984B38" modified="2019-11-13" version="1">MVVFALWVQEDESRLSPHENKNSAGLEVDDPALDVSNYQTTQSWTACEDLATNGKDNHDFMDAGELNTEDNAFSDHELDSCASDLDTSSDLSDEANSKDFELSAEDLDLMRELGRYMISSLFIGYAHAILSQSIYDRIRSMFSLMDVQKSYSILGNPTYTLSIQKILAQEVGNPLVTRAMDYYPEDARGKNIFKLSQSKKWLEDMDPDSRAPMVRTTNGDFYLFEPVQLSNDSVVVPIFFYKRGEDLWSKCVTPAFDPESNGHKLRICIPWPVNFNSDIAHKINISEFYCAFPNIAGPDGLMLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIP4</accession>
    <name>A0A5B0QIP4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015969</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIP4"/>
    <dbReference type="OrthoDB" id="2500898at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="186" mass="20587" checksum="79F7E76C0F0F0F1B" modified="2019-11-13" version="1">MPCPHKRTTSTQDHKPGDENNPNSSQAASGIVPARRLTDEEELKRAQKIAENALMKKTVFRCGSKINRPSSDSSCSNLLKHASICQRKEKESKENRQLADHGFTASGQIHPKEVPQLCAIWCAQAARPFSALTDASHKAILHQTVVKNLLTPRGVSNDIHMLYLAIQNNYHNVLNTHNGAMYLGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJX1</accession>
    <name>A0A5B0QJX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJX1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="431" mass="48090" checksum="CCDEF0BEED1EB55C" modified="2019-11-13" version="1">MSGKVKMPKSEHIHSESYVQKSEISKKLCHFPTNQQIDDLLSIAQKRADVLISFAGMQKIPGVTIGEISDLSDSDITSTPDDVSSSTTKAYYYKPNATSIDDPSDNINNAMQDAAVVASERQELENNLLECEATHEMLENELVQTSRISIQNLLNALSSTNTLTSSEKPGNQEFEDQSSLDLVKDNKLNQENLVCLRHNHDSEIQVHHGNERRKRRSNTQPSSEEPPAPAKLDPKICSKIVSICLKDADAQQNTMARMHQWNIPIQLNLKTLSKHTSVNLDFSAHNLFATGQVDKENPLEPGKFAAVITKGASYFSTFFKNGRHEYTKASDTRAKLSYIHVQLFNYSSTSPIALGYNLSTPDRYTFALINTHDLHFLFRETEGMTITQMDPSEACVWVPSPLRVLLQAMSSSAYIKKVEADVAPCVPKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QK25</accession>
    <name>A0A5B0QK25_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033812</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QK25"/>
    <dbReference type="OrthoDB" id="9995306at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019519">
        <property type="entry name" value="Elp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018627">
        <property type="entry name" value="ELP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184:SF6">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10483">
        <property type="entry name" value="Elong_Iki1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="227" mass="25880" checksum="63D8235D0CB08B78" modified="2019-11-13" version="1">MNFPLEDYSIPAKLSLLLVDSIDSPANFILLQLISNALRNHRAVILLLTDHDQNYWLSLLNKNRVNQLLLTIIQPKLDEDIKLLLTQQLTKSKNKNPLVIINNLSSIIWNNNLPTQQLTQSFKALLPSQLNHDFSLVYLFHNDLIKNNNNRDREIFIQLINSSDIILRTRALGGQAQTQGELIIQRGPAYLGDLNLPITTSSDRTVQFKIQDHSVSFHPKGLDKAFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNZ3</accession>
    <name>A0A5B0QNZ3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNZ3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022812181" evidence="1">
        <location>
            <begin position="23"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10494" checksum="C99DA537AFFFE231" modified="2019-11-13" version="1">MVTIHHLVTVLSLSTVAPVITARPPCTKCINTVRTNFLEVPGECTENLVCKSGFDHGKCSGEMFRRCEGCHKPFNARCSKMGTKPHNEYDCGTNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ69</accession>
    <name>A0A5B0QQ69_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ69"/>
    <dbReference type="OMA" id="PLMGASW"/>
    <dbReference type="OrthoDB" id="2498375at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022708106" evidence="2">
        <location>
            <begin position="21"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="229" mass="24689" checksum="649DA2218793CB70" modified="2019-11-13" version="1">MVSLAYLITLLSLLVAVSRAEEVAAGKTEDVDPKYWGYGARYGSFYNRFGNCYSWGCGWTSGLMLNSWGVPWINSLFNNYNCYGGGWLLRGPGMYFVKATEEAHSVARRALPVSAEQLVRREAGVTCISNKGVSSTFSPNECIKAAHELAEKKTSSATHGSCTLTLVTKNDKVAPSQSSSQILEKAVNSILGACGKTSDTQSQNKNARRAAAAGQEDDTRVALLLSKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QT03</accession>
    <name>A0A5B0QT03_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013968</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QT03"/>
    <dbReference type="OrthoDB" id="2507377at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409:SF1">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="202" mass="21804" checksum="DBEEFE0BB9D118A5" modified="2019-11-13" version="1">MGNPASSAPAATNLRPSPVVPQAHHKPHGASATSSARSTNTAAGSTATPAAQGSAAARRAKAAQAQAALHTKITLFFNPEARAARDQETSMSHFYAARLQEANSSIVRLQDEANRLRDGVNLQVIRLQEEVQQARLNLASKTAENQDLRHRIEMLQLRLEMQHTNGFGMPGMMQAPMFVQSNAHQNPSAWEVNTSSSNPRAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QT41</accession>
    <name>A0A5B0QT41_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Rsp5p-dependent ubiquitination, sorting of cargo proteins at the multivesicular body</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SSH4_2</name>
        <name evidence="8" type="ORF">PGT21_014126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QT41"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd12910">
        <property type="entry name" value="SPRY_SSH4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035780">
        <property type="entry name" value="SPRY_Ssh4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="142"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="375"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="570"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="375"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="570"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1116448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="598" mass="66471" checksum="DF44CF9523584923" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTW7</accession>
    <name>A0A5B0QTW7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_022963</name>
        <name evidence="7" type="ORF">PGTUg99_003168</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTW7"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="49"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1080831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1116722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="160" mass="17306" checksum="EC4FAD0A6178A4DC" modified="2019-11-13" version="1">MSFLIAPSQWDDFEQLFDKLVTDRYGPPSSDRAKGMNDSKALGSTGSHMVSRIQRPKMDVVETDGSIVVTTELPGAKKEDISIDLQNGRLSISGHTKASSEHSEGSVRVSERSFGSFSRSIAVPPGLTHDQVKAGFKDGVLEVTIPKTVPNKESHNIPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QU39</accession>
    <name>A0A5B0QU39_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031071</name>
        <name evidence="3" type="ORF">PGTUg99_021598</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QU39"/>
    <dbReference type="OrthoDB" id="10268199at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366542" description="Hydrophobin" evidence="1">
        <location>
            <begin position="23"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9727" checksum="C1612F71B7015E1A" modified="2019-11-13" version="1">MNVFQSTLPIIFLAGVCKFASARCGTGEVDACATYQQRIYQAPPGNFDCHITSSETHYCCPANEMVPSTPDYPLGYAIKTLKCYKNTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWX9</accession>
    <name>A0A5B0QWX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022809</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWX9"/>
    <dbReference type="OrthoDB" id="2505268at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023131837" evidence="2">
        <location>
            <begin position="21"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="171" mass="17829" checksum="DC64AB8E32A7A0B7" modified="2019-11-13" version="1">MNSSVSLMMIVLLSAAATMAVPFGYAYGPQYYPGPEYYPGFYAGDPYYGPGGGYYPYNTPFPGYAPYGPDRFSAFAPYGPGIPVYGRSVAASGAPPNTNPAQVLGRATLGTQDMPAKQPAGPNDQTPGSQAQDVQRDSQLPVGSPDSHGPMGLGQSQETAQMEQSKPAMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXW8</accession>
    <name>A0A5B0QXW8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXW8"/>
    <dbReference type="OrthoDB" id="47375at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002654">
        <property type="entry name" value="Glyco_trans_25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15114:SF1">
        <property type="entry name" value="REPLICATION PROTEIN A 14 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15114">
        <property type="entry name" value="REPLICATION PROTEIN A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01755">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase family 25" evidence="2">
        <location>
            <begin position="95"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="370" mass="40941" checksum="666B336F5D81FF62" modified="2019-11-13" version="1">MYSKASHHQSSSSGSSPWLVIALFISLTLFLTQILPNITTTGTAFRSQFPGWPGSGTALSRLQDKNNGLRTTTTTKTGMVEALQAQLPSFSSVRVVSLKSRTDRRMHMQTLADFLQLDNLHFVDAVLYSDPRVLEIVQRIGNNTKADKKIAEVGHVACRMSHRLAIEAADTDDDDITLILEDDVDIEAAFKYLAGTILRDVPKDWDIIFFGHTDFSNESRNGPDPSTHNFYIYKSVEPQGGHGYALSRKGRKLVLDLMDNKRPEVYETDAGQPVDEIFVYLARLHRAALYSIIPDLIVQVPLFVSDIFGTPAGFDDGMHAKKLLDSSLTRIAAAAASSHLSPLNHPVISQSPALGHSLSRRTSHRHAYTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYF1</accession>
    <name>A0A5B0QYF1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Iron-sulfur cluster biogenesis chaperone, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HSP70_2</name>
        <name evidence="11" type="ORF">PGT21_032105</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the assembly of iron-sulfur (Fe/S) clusters in mitochondria. Assisted by the DnaJ-like co-chaperone jac1 and the nucleotide exchange factor mge1, it mediates ATP-dependent Fe-S cluster transfer from the scaffold proteins isu1/isu2 to grx5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYF1"/>
    <dbReference type="OMA" id="GREQKMT"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11734">
        <property type="entry name" value="ASKHA_NBD_HSP70_Ssc1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000020">
        <property type="entry name" value="Chaperone protein HscA homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000003">
        <property type="entry name" value="Heat shock protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000007">
        <property type="entry name" value="Heat shock protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="649"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1118129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="677" mass="73486" checksum="619665669ADC9B09" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZJ7</accession>
    <name>A0A5B0QZJ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_003465</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZJ7"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228:SF2">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_4G14350)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022893619" evidence="5">
        <location>
            <begin position="19"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="469"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="599"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1118721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="609" mass="64878" checksum="A5EE2ADBC3C408C0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZU9</accession>
    <name>A0A5B0QZU9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015996</name>
        <name evidence="3" type="ORF">PGTUg99_009236</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000246">
        <property type="protein sequence ID" value="KAA1118639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZU9"/>
    <dbReference type="OrthoDB" id="10353731at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="140" mass="14608" checksum="AB1652223F6614A9" modified="2019-11-13" version="1">MPNNLVNLSTQMRDANTPGTNDVQPTPANEQSSNPSVDSTATPSSTLLVPNPTLPDPTNNSSENTNTQNPSQSVTPSANHTATTTENSNARESQKRSRTSKSNPNGTNAKESGPTGSDGSQRQNNQPNGLATLLGAKHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R076</accession>
    <name>A0A5B0R076_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002210</name>
        <name evidence="2" type="ORF">PGT21_030168</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R076"/>
    <dbReference type="OrthoDB" id="2518936at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="591" mass="63574" checksum="E18BD32EBEB12C30" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1V8</accession>
    <name>A0A5B0R1V8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1V8"/>
    <dbReference type="OrthoDB" id="2498268at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26615" checksum="7090862F69037946" modified="2019-11-13" version="1">MNTAALSQLLQEIFLVHSPSEEHSGSAETSVYINPENSDEYFLITMQKAYSWAKAIQANPEEVTVLLPPKSPIFQFKNKNNKSEQPNPPTFPNNSQPQQFYPNPNQFTMPMAYQTPMMPYGNYSGAFGNSHQANPSAFYPQASTSAVQIPPSKSNVIDPSPPPFEDTTDLSDYLKFARVDADSEEINNGLRKLGITHWSMFEVVSADELVTSGVPLVPARCLIRSVKTYPTHLKKIKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R270</accession>
    <name>A0A5B0R270_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029841</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R270"/>
    <dbReference type="OrthoDB" id="10299931at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="243" mass="27162" checksum="372B1B4059B5A439" modified="2019-11-13" version="1">MKSAGAGVRYLSSFSMSRKRHTAFFAPLVFSQVVMHFSSFQATPVILNEPAEQLLGWQPGDDLTKGLPPFESDDPSDWNLDAWEDGIGSQLAQWGKTDPIQAPHPQQTHDGQETTLLSPALPSFENIKSGAGDSLEAPTIFYPLGSDGGRLNTHYRPHPVEYQSLLELEVPNKLRPRDKPEDIQFSGQTDKRYRPGLRFSERYQAGTSALDQRYCTGPFPDVVSKKPKNQVNFMKDEVDHLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4M9</accession>
    <name>A0A5B0R4M9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mitotic spindle checkpoint component mad2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MAD2_1</name>
        <name evidence="9" type="synonym">MAD2_2</name>
        <name evidence="9" type="ORF">PGT21_006203</name>
        <name evidence="10" type="ORF">PGTUg99_019915</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000244">
        <property type="protein sequence ID" value="KAA1120448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4M9"/>
    <dbReference type="OrthoDB" id="1806at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF11">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="8">
        <location>
            <begin position="43"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1080418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1120448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="261" mass="29282" checksum="5C13F814F9F34395" modified="2019-11-13" version="1">MSSVPTSRRTAGGTATRVRPARTETANKTTDTTTKTKKRINLAGSTAVISDYFHYAINCILFQRAIYQQSDFKMVKKFGLNMMIVEDESIAEYLKKIVDQVRVWLIEGKITRLVLVIQQKSDGETVERWEFDVTVEEPPLDDAKNNVSQPSQAPASSSSKKDKQAGSKTKSKSLAEVQLDIQEIIRQIISSVSFLPIPTEPRTFKVLAYTHSVGQDEATEWNDSDAFDITERAERVKLKSFSTAIHRVDTAVDYKLGESII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4X0</accession>
    <name>A0A5B0R4X0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_037088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4X0"/>
    <dbReference type="OrthoDB" id="10378580at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="281" mass="30112" checksum="FC7C6BE58E818EF2" modified="2019-11-13" version="1">MDKIHKDYPHATGHPFFGGLISNLLESINTDTPKLVESVITNKPISQLAKHPKVQEEQVASLLPATIDYKPASSQGGTQAPAGSDMVVNPESTTVSQKKLKLKLKWSTPSAPKEHKEADTLGSGGLTGNPYDIPLHPLWAVSCCTTVHHTSSTPKDGNWAGFPTGRLNPTGFGPGSGQAFHGIFYLGPARDDPIKVPTPSATPPPDQARGQPAKSPEAHLYIGLWAARFVNGFGSGCRFLECNPAQSARHFSGPPWASPRAARARPIIILKYSNLRLDTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R702</accession>
    <name>A0A5B0R702_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BRCA2 OB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_023273</name>
        <name evidence="5" type="ORF">PGTUg99_028347</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1121039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R702"/>
    <dbReference type="OrthoDB" id="21095at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000406">
        <property type="entry name" value="Breast and ovarian cancer susceptibility protein 2 truncated variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000579">
        <property type="entry name" value="Chromosome 8, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015525">
        <property type="entry name" value="BRCA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015252">
        <property type="entry name" value="BRCA2_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036315">
        <property type="entry name" value="BRCA2_hlx_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015187">
        <property type="entry name" value="BRCA2_OB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289:SF0">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN BRCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09169">
        <property type="entry name" value="BRCA-2_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09103">
        <property type="entry name" value="BRCA-2_OB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81872">
        <property type="entry name" value="BRCA2 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Breast cancer type 2 susceptibility protein helical" evidence="3">
        <location>
            <begin position="777"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="BRCA2 OB1" evidence="2">
        <location>
            <begin position="831"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="912"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="914"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="924"/>
            <end position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1105887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1121039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1241" mass="136459" checksum="473F02290B0B5E2A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RAG2</accession>
    <name>A0A5B0RAG2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013474</name>
        <name evidence="3" type="ORF">PGTUg99_026997</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RAG2"/>
    <dbReference type="OrthoDB" id="10328313at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138100" evidence="1">
        <location>
            <begin position="25"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="152" mass="16677" checksum="07F2D20005A875B4" modified="2019-11-13" version="1">MKSFIFVTSLWMISLIASTGGSLATGPSSASRGKGVMDTAENTVKNTVKWEPEERYYMRDAKALAAWDLGVTAQLLARSGKGAKALHKVDTGGVSGLFRFFTKETGSLMLSSGRHDIVYTLYDQAAKMVSWKNTLKRGVGRLFKIYKVDEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RQR4</accession>
    <name>A0A5B0RQR4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028461</name>
        <name evidence="3" type="ORF">PGT21_028674</name>
        <name evidence="4" type="ORF">PGTUg99_009756</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000146">
        <property type="protein sequence ID" value="KAA1127967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RQR4"/>
    <dbReference type="OrthoDB" id="2511290at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1127967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="66" mass="7160" checksum="7C0A70AC91910C52" modified="2019-11-13" version="1">MARVRRFTCSSKPRSLSDGPGRGGTGNLLALTAPHTRPHTRIRAIDEPLAVSTLAHIATKRARRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RSG2</accession>
    <name>A0A5B0RSG2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002031</name>
        <name evidence="3" type="ORF">PGTUg99_001177</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000149">
        <property type="protein sequence ID" value="KAA1127793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RSG2"/>
    <dbReference type="OrthoDB" id="2501730at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="778"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="995"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="518"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="719"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="827"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="882"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="942"/>
            <end position="955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1127793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1048" mass="110204" checksum="4C481200FD5AA9D8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RVJ8</accession>
    <name>A0A5B0RVJ8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_002126</name>
        <name evidence="11" type="ORF">PGTUg99_008330</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000115">
        <property type="protein sequence ID" value="KAA1129991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RVJ8"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="9">
        <location>
            <begin position="338"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1093895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1129991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="489" mass="56583" checksum="E7CB91B118648439" modified="2019-11-13" version="1">MNSTRFVDEMDRFQLPTILKREYEYCQSLFGQRMVLHARRVNFKPVIMPLTTSFFFLWLCAVSLPEKGPLPKLFRLSVLPFLLASAFDIAVNKTYSLGSPLRDVMFPYWGCLIAFRIIDMALVSLWDEDPAPRWIKPQKLPEQENLHVAKSVISLKITEEKDVEEVSRALFDEGKGTWKSSRGRRYLWRPVPHSPLLSFDRALYGLDIILLNRPGTPWLFPWRLRSLEWSLVALNGQRREQLNFGKPEWPFLLALIQQVFHLMAHNYIRSMDLDGKRTETRDLWAIKLPDQILVTLALGAIVALSSSLEEAITFPLLLNSKFLPETALLGNTRRAILSKGLAELWGYRWHHNWRRSFVRTARLFPGGLNPTWNALWTFFLSGMMHSIMLARAYPTPTLSRPCTILPIFWEPGMMLFFMAQGIGTLIEKTYFPTKNASILKRIWMCIVLIGTGRYLVNSMVVKGWLSAYEWNQMNYGNVISDIFGRSISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RY50</accession>
    <name>A0A5B0RY50_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Programmed cell death protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031795</name>
        <name evidence="4" type="ORF">PGTUg99_013914</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000106">
        <property type="protein sequence ID" value="KAA1130760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RY50"/>
    <dbReference type="OrthoDB" id="10252486at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.140:FF:000006">
        <property type="entry name" value="programmed cell death protein 5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="13"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1130760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16421" checksum="85AA0D7437785D07" modified="2019-11-13" version="1">MADPELEAIRQKRLADLASRDDGAASSVPSSVMSKLGGQPAGSGEEEAKKAENEEIRRTAVSQIIDNDARERLSRIALVRPQRARQIEDMLIRMAQSGQLRGRVTEEQLISVLDQLERAESGSTASVNQPKKITYVRKDAFDDDDWDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S9P8</accession>
    <name>A0A5B0S9P8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007376</name>
        <name evidence="2" type="ORF">PGTUg99_031659</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S9P8"/>
    <dbReference type="OrthoDB" id="2514281at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1134189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="268" mass="30662" checksum="C1B0CA8117CBD24C" modified="2019-11-13" version="1">MGSHLGTQGKENIIKALQNRRPAVKKIIDTFNGLYEQFQQKYPEQHLSDAHNHPLTYDDFVEWEMDHPFWNDGLYHHTEAPWSIDPDVKTGINCILVLRRVQEEFELIAQELARAVGWAIATHSQLTETIVHITTRINLVKSDPNVATDRFDDLSFGVVSRLGKLELVQEELKLSLIDHGKLIEDWNDQVNSLWRQCQPSGNSAQFQQWTELIDKVKVDQSTVFPTLSAFSQPQNDVEDALEEAILDVGLDDGEDADEEWMSEGEEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SBX9</accession>
    <name>A0A5B0SBX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025639</name>
        <name evidence="3" type="ORF">PGTUg99_005211</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000042">
        <property type="protein sequence ID" value="KAA1134999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SBX9"/>
    <dbReference type="OrthoDB" id="2507502at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="650" mass="73767" checksum="2CE759C781435C9E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LM72</accession>
    <name>A0A5B0LM72_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SEC21_3</name>
        <name evidence="19" type="synonym">SEC21_1</name>
        <name evidence="20" type="synonym">SEC21_2</name>
        <name evidence="19" type="ORF">PGT21_015260</name>
        <name evidence="20" type="ORF">PGT21_017229</name>
        <name evidence="18" type="ORF">PGTUg99_019363</name>
    </gene>
    <organism evidence="18 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">21-0</strain>
            <strain evidence="18 22">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cytoplasmic vesicle</location>
            <location evidence="13">COPI-coated vesicle membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the COPG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LM72"/>
    <dbReference type="OrthoDB" id="1074925at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000046">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000071">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1480:FF:000001">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000008">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1480">
        <property type="entry name" value="Coatomer, gamma subunit, appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032154">
        <property type="entry name" value="Coatomer_g_Cpla"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037067">
        <property type="entry name" value="Coatomer_gsu_app_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16381">
        <property type="entry name" value="Coatomer_g_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="15">
        <location>
            <begin position="18"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer gamma subunit appendage Ig-like subdomain" evidence="16">
        <location>
            <begin position="668"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer subunit gamma C-terminal" evidence="17">
        <location>
            <begin position="816"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="635"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1065425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1090840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1110295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="930" mass="102762" checksum="A6DC2E4B5F2F7F83" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMJ6</accession>
    <name>A0A5B0LMJ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_023863</name>
        <name evidence="5" type="ORF">PGTUg99_008632</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMJ6"/>
    <dbReference type="OrthoDB" id="2509396at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036136983" evidence="3">
        <location>
            <begin position="23"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1066167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1072646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="277" mass="30679" checksum="E97ED59805768908" modified="2019-11-13" version="1">MVSLFLSVGLLLFFQKFYQFSAYGGPVSNLVDTPDSASRVSITIEGHVEPSLQSRPVADSLVPKEGNPEGDSNELPGATNHQSDNLQGCHGENGEDELNHALKISKEEYEKQEQQEDHLLEEALVASLLDHSKKISEDALSDSELEQFQESTKEHNKTLEMENQDLAHVLEISRNEDLDRELQISLSRALHMSLEDGNVSGTSHDGESDIEEQIISHVLKMSENKNSNQAKNKMDEPLTQQTPKSEEPTHKSPDAGSKEDQDLHGSGSKSEDPDQEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LR19</accession>
    <name>A0A5B0LR19_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LR19"/>
    <dbReference type="OrthoDB" id="2501395at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="31499" checksum="328E5E2327C7C99F" modified="2019-11-13" version="1">MTGKEPITSDSLSKDNSTENQKLTTTTSSNGMEKINATVLKTSIEGIPLLTQDNYTHWRRRVINLLDLIDMKISVTTDDGILSDADNKLIKSILVSKLESSVQANVINATNENNAKLIWTSITQFFASDESSNKARVFRSFLRTPFTPNDIAGFITTMKTFKTRLIEVGWSLPADSIGHLVMDKFPASLDNIANVITHTGKEITLDTVVDHLRLHAHNLESRAIGNGTKSDPITLFTDSSRRCKRNAHNTLSSHSESNCWMLHPELQPAKKDSANKTEATNTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWI2</accession>
    <name>A0A5B0LWI2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWI2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="647"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="693"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="756"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="767"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="788" mass="74719" checksum="3C440D31861CAAC1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYE8</accession>
    <name>A0A5B0LYE8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYE8"/>
    <dbReference type="OrthoDB" id="10614665at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="187" mass="20728" checksum="39D606471F3A0877" modified="2019-11-13" version="1">MSDNSSFDAETSSINNSDEEISSTEVSSDEQASSKQSFNTSRSSHSETQSNQSVEEYSSTSDQDEDDETASDNEESFVTDREEIFTSDDCSENSGPDDPPYSFNSDTQSDSSKSDSAPPDTSEQHQKNIVQNLLSTGYKGPHILKILWDEHGIQMSARTLSRKRKEWGLRLCDLEQAPPPTPLSLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYF4</accession>
    <name>A0A5B0LYF4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018592</name>
        <name evidence="1" type="ORF">PGTUg99_050230</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYF4"/>
    <dbReference type="OrthoDB" id="107110at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="849" mass="95586" checksum="7BB76A29608EB288" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZ53</accession>
    <name>A0A5B0LZ53_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033567</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZ53"/>
    <dbReference type="OrthoDB" id="10410834at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="497" mass="54910" checksum="08DF5275F157B092" modified="2019-11-13" version="1">MRSVLASGKISRRRLSECASSWSSLGFFLHLTEEGTLTPLLILGTKLTHWGSVPLCRATLVSLVVILILHAFIAATCLLLLTLPCIRPLKRSPWYLKKLYIEDHSSGKAHQVPLYFANAGVLLTTSQLLSSASTSAFIWFDIGAASPGEHLFGSRALPAVGLMNLAEILAYWSLSHCFLVITICHGNKKQQEKSMSSIRHIPPQVINFIFTFVPIAAMVITIFVIAWVTSVTSEIQSYMKQGLMALDQGSSIWKQLQATPQSLEAQAQLRGITMQLANLFRNAFPRLEVAFHRFRLGECLFLSFESVSCPIFVASFWILVQNYLKQSPNSTIKSCSSGGSMTHMNTGTESGTRTNTHSVVASRSYLDAIKTDRQFFHLNIRALGTFISMLLNIILHMVNISRTTDALTVPYWRTVATLLGTVGSIFSGIPVVWQCWRLHVDQSDATADCSNRYALAVVKARESHVPLDASGARGLKVPVTIHFEHSDTLVAPKPEMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M388</accession>
    <name>A0A5B0M388_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022829</name>
        <name evidence="2" type="ORF">PGTUg99_009214</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000175">
        <property type="protein sequence ID" value="KAA1125734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M388"/>
    <dbReference type="OrthoDB" id="10274719at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1125734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="436" mass="49875" checksum="F623F6DE27027B10" modified="2019-11-13" version="1">MRQEMQDQLNWVLTGFFVNINHHTLELSQADIASLQNHGSDISNAYHVIVTPRAEKPGLLAGIGSGTGDLQKSNAKLVLHILGKIEESCIILEDPKRFTTAKVNVNALKLMYRLNDFSVGLLAALEKHSLISDEFIRGILNDRYRGKLIFNWTSGVHVFNPAHFQVLYMSIDFRLSMKSSPSTHLIHGLLKHLDSVTWKNLERSCLDAQLTGISLDPELEEHMSSFKQLIQLRNEFMALTSPAVERLTDHAFQGHIHQVLQDLIRRIFSTVDGEALDHYLIRIKLSYNVLRFLIEYCVKPSRLSEEYMRKLRKLADFPKLQALEIYMIEYSDILQKAYVELGETLQRSPHRELTEKYGLDLLDGRYLVDMDKTEQPLLISLGKNKDTNMQTPKLESLEIINELKKEIELVSNTLEFPNQYGVESQAKNLASALGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M463</accession>
    <name>A0A5B0M463_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003068</name>
        <name evidence="4" type="ORF">PGTUg99_008869</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000015">
        <property type="protein sequence ID" value="KAA1136936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M463"/>
    <dbReference type="OrthoDB" id="2512322at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1136936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="504" mass="54360" checksum="029455D281B0198B" modified="2019-11-13" version="1">MVVRRLPGGSDLGTSTGSNSSAPASGQSASYQSTGTPSTTTSLTSAPSAQATGTATPPSASSSIASTVTSTSSQVPTASPTPVTTQTTDLSASPTPTNVPEQTITASTEGVAESATLPSSSLTEFSQPISSAPVPNASAAQQSPGQTSEFTGTIITAVCFGYFTILFATVVAVYILRRRRHQEVDHRTSRKISKWRSQSKKLNQSFDFGESVYNPQTPSLRVTTPFTWGDCSTKNLIISDLRVNRHNMTPHTNSLSMTHSNSASMFSARSSASTTNTVLDPFMRYKYPRRQSLASRLPQSPSTAYSRPEGSKYSSLSSAFTSFICRKSHFNGGSSYWSSEDAKSREKFELLNRRLRSPSRCSTFSERSFNMVAAEGPSYRRTSRYGSDRKSDLLIETETRQSGITSLLENYAYDSGPPQYPSSAGVLKKSRNSIEKAARNSLGMTLVFSDTASQMQSSYSPGQDLCMLGLDFSDGMPDEPSPSTCSPGLKSSYLLEESRFKKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7M0</accession>
    <name>A0A5B0M7M0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7M0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="203" mass="23545" checksum="7CE506F867CA6F0D" modified="2019-11-13" version="1">MSGNEKDDTKEEKDRTTGSRMMVSKSSLFTSVFAFCDYFLHLKTEETPSIFSRPCDLSYRCWTWSLPIPFSFRLFEQLDWKRNHFNLLTKLGQTATIKFQAGVANPRPDLLLSVGRRRRIRLQDTRVESKQALNSDLIESRYRAVRPFTLQARHHVHWRVSRNELDSDILHSKLSGQSRRLFHSGGATAVSDKTPIIYSLPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBW4</accession>
    <name>A0A5B0MBW4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dipeptidyl-peptidase III</fullName>
            <ecNumber evidence="5">3.4.14.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Dipeptidyl aminopeptidase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Dipeptidyl peptidase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_010707</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.</text>
            <dbReference type="EC" id="3.4.14.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBW4"/>
    <dbReference type="OrthoDB" id="4694525at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF11">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1074577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="179" mass="20282" checksum="9B08E8E6922EA98C" modified="2019-11-13" version="1">MWVMEPLETPTLYAIIIVTESYLGHIKTYVYPFAQCAEWEGFTAIVNKEFSQKFELLVNNAQHLVQTLPWGPPFEVNVFQKPDFTELKILSFATGGIPAGINIPNYFDFRESTGFKNLSLVNILSAKAANKEITFIHPSEPEMYAKWDAKTFDFQVANHELLGHGSGKQLTQNEDGTFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCX9</accession>
    <name>A0A5B0MCX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCX9"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038921">
        <property type="entry name" value="YOR389W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204:SF1">
        <property type="entry name" value="ENTEROTOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204">
        <property type="entry name" value="YALI0A21131P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="416" mass="47237" checksum="A8F9A1570ECFE1EC" modified="2019-11-13" version="1">MWCDFTQGVKLLRSTNTTDPFDAYSDPRESQASIKSSESNDSAEEFLLNRTDTFSPLLSDGIQKDFHDQKIEVIGAKNGSDKIHGPENHEEIKKSGNRTKNLMAHVTRQEPINSPFYLRGPAYYYRAASLQFFWPGEARVILDPSGFISFYDRIDSLSEKRKAEGTVNGPRVAHRLDGISAHDVQKIKDRLLNVLARKNAEDWQIDSDRLDWRTLVNTIVQTYSSPLAELEFLLQREDMTVIERVVEVRGLTYGIILPYVDFSSWNDTGTTKDPTWLQEGIQKCAKGFTAGNYDRSSEPITESIEVIIGAIEGTLEHLCGTIFGIFSQTIEMALPIDSLVTSDSTKESHAESRIGQWKQQIKELMVWLGWSTWGHCDPPCKTNELCLPPLWPLFWLEGFPKFDQFPFCKIISGKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDF8</accession>
    <name>A0A5B0MDF8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_030570</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDF8"/>
    <dbReference type="OrthoDB" id="3229693at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010435">
        <property type="entry name" value="C5a/SBT2-like_Fn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF66">
        <property type="entry name" value="SERIN ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06280">
        <property type="entry name" value="fn3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 9 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022718815" description="Peptidase S8/S53 domain-containing protein" evidence="12">
        <location>
            <begin position="24"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="13">
        <location>
            <begin position="148"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="14">
        <location>
            <begin position="404"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C5a peptidase/Subtilisin-like protease SBT2-like Fn3-like" evidence="15">
        <location>
            <begin position="637"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF06280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1075177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="931" mass="100551" checksum="84626570F4C85E83" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MI58</accession>
    <name>A0A5B0MI58_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pru domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_002788</name>
        <name evidence="9" type="ORF">PGTUg99_026940</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MI58"/>
    <dbReference type="OrthoDB" id="340431at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.70:FF:000001">
        <property type="entry name" value="Proteasomal ubiquitin receptor ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.70">
        <property type="entry name" value="Proteasomal ubiquitin receptor Rpn13/ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044868">
        <property type="entry name" value="Rpn13/ADRM1_Pru"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038633">
        <property type="entry name" value="Rpn13/ADRM1_Pru_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032368">
        <property type="entry name" value="RPN13_DEUBAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038108">
        <property type="entry name" value="RPN13_DEUBAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16550">
        <property type="entry name" value="RPN13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="7">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1076331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1135622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="285" mass="31298" checksum="E4746DDEAC513916" modified="2019-11-13" version="1">MTRLFQFRAGRCERRGETNIVDPLPSKGLLYVEHNEDDGALNHLCYKDLESGAVIDDFIIFAGDASFQKVLVPDSATARVYALCFSSSNQKLLYWMQDPDHTTDAANVARLNQLIVDEEQMSMEYPATADGNRQDSPAQAAIRPEAPAAPQGVSVEQMNQLQSIIAGFGQNSASIGQSSFRLGDILTMELVEPILNDPEILSSLQSYLPPGIKPDPESVGRVIRSSDFKAAVENFDVALRQSASGNRDDGLMMFLLHGLGLNADQCEGVEDYVDIVLKLNDDRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIH8</accession>
    <name>A0A5B0MIH8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_029206</name>
        <name evidence="6" type="ORF">PGTUg99_025601</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIH8"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF28">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1075876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1091227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19663" checksum="DA7DDF1D1F07ADBB" modified="2019-11-13" version="1">MSYATRHCSISRHNNLRRTKVWFYPPEILPQRVRAQGTAIATACNWALNFFVVECFPLTTEKIGWKTYLIFMALNILFVPIMFFYFPETKGKTLEEIDLMFGDTVSAERRRCTLHKIQHSLRGIDTLGEHGKSILDYKVRNVQTDAEKSDRGNVDATAIYLSHKKGTISVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKZ4</accession>
    <name>A0A5B0MKZ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014877</name>
        <name evidence="4" type="ORF">PGTUg99_025222</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKZ4"/>
    <dbReference type="OrthoDB" id="2507780at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366154" evidence="2">
        <location>
            <begin position="23"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="449"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="536"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="598"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="757"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="770"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1126822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="811" mass="79199" checksum="A5299BAE7AB6BEA7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPJ0</accession>
    <name>A0A5B0MPJ0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">RPB1_2</name>
        <name evidence="18" type="ORF">PGT21_032325</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 20 21">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02584">
        <property type="entry name" value="RNAP_II_Rpb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02733">
        <property type="entry name" value="RNAP_II_RPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.140:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000684">
        <property type="entry name" value="RNA_pol_II_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007075">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007073">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038593">
        <property type="entry name" value="RNA_pol_Rpb1_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF37">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04992">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04990">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05001">
        <property type="entry name" value="RNA_pol_Rpb1_R"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 15 18" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="17">
        <location>
            <begin position="250"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1560"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1563"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1596"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1670"/>
            <end position="1745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1746"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1764"/>
            <end position="1775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1776"/>
            <end position="1795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1078273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1795" mass="198357" checksum="F5D61DCE8337BCAB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQR9</accession>
    <name>A0A5B0MQR9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023580</name>
        <name evidence="3" type="ORF">PGTUg99_014524</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000446">
        <property type="protein sequence ID" value="KAA1078882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQR9"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF342">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="62"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1078882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="588" mass="66085" checksum="0B58F85EC9D4BF1F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MR39</accession>
    <name>A0A5B0MR39_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MR39"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="136" mass="14508" checksum="0E54875EB60E0F48" modified="2019-11-13" version="1">MAGVATLAHHLQGTDPATSIILPPLHNHPAQLKLPVKGQRKIGAALNPKGCRLRDKKIPLAGHPEWICTLSIGIVTEKGAPHSETNGSSTAAWSIQREAAEAGSLCRCRNAPFGRACCATLNAWWDRRAVLILPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MT33</accession>
    <name>A0A5B0MT33_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Beta-galactosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_004721</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MT33"/>
    <dbReference type="OrthoDB" id="408320at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051913">
        <property type="entry name" value="GH2_Domain-Containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Ig-like_GH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42732">
        <property type="entry name" value="BETA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42732:SF2">
        <property type="entry name" value="BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="beta-Galactosidase/glucuronidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022949404" description="Beta-galactosidase" evidence="5">
        <location>
            <begin position="29"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolases family 2 sugar binding" evidence="8">
        <location>
            <begin position="117"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 immunoglobulin-like beta-sandwich" evidence="6">
        <location>
            <begin position="236"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 catalytic" evidence="7">
        <location>
            <begin position="380"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="638"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="647"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1079234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="668" mass="75075" checksum="77CE43CFEABFBE37" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVB7</accession>
    <name>A0A5B0MVB7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_003213</name>
        <name evidence="6" type="ORF">PGTUg99_016856</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1079978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVB7"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF386">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="4">
        <location>
            <begin position="55"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="370"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="381"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="399"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1065559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1079978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="598" mass="66486" checksum="850906D7C18A8F25" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZD7</accession>
    <name>A0A5B0MZD7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031357</name>
        <name evidence="3" type="ORF">PGTUg99_033001</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZD7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="140" mass="14855" checksum="F8CEB313D51D8982" modified="2019-11-13" version="1">MRFDHLHLTALSSTELEDQPAQAVGSFHPPPPPPPISHSPAAAAKLVRGPSLIPAENSGQATGTSSAVNQLIRIQSKATTDKKNLTGHELTAQISSGSLAITIRSSAAVVINQAEFTRQSRYQCHSTPDNCAFLDFQDNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZV0</accession>
    <name>A0A5B0MZV0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZV0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="134" mass="13555" checksum="45A169CEC03F69AA" modified="2019-11-13" version="1">MIHTSDYETPGFSNCNANYDLPVGAYPPPGPKTFHQGDPTTPLAVAAPASSQCVSVPSPVPQGVTYYWAGGAPAATATATPNGHSNSNSNSGSKDGAKTGAASARRSPVNSESQFFPALTTIALSAGLLMVLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0Y3</accession>
    <name>A0A5B0N0Y3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_034342</name>
        <name evidence="9" type="ORF">PGTUg99_008347</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0Y3"/>
    <dbReference type="OrthoDB" id="1507364at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000070">
        <property type="entry name" value="Endoplasmic reticulum protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF3">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="123"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="55"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1066604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1081790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="478" mass="53184" checksum="B6781C9729A2A9AA" modified="2019-11-13" version="1">MEGNRDEAKRAFKLAQSLQSTDPIKSLKFAKKACALYWSPEAAALVKTLETGEGPSTTTTQNPTATSTATNTSSTTTTKRSTQPSTPKPSSSNKNPTSEQTPPTYKPAQLELVKKVRRHKITQYYEILQLKREADESQIKSAYRKLALALHPDKNNAPGADEAFKMVSKAFQVLSDPDKRAAYDRHGADPDSRSAGVPNSSPFGGGGGGGMHFSHEDAIDPDQLFRMFFGGGGFDHPGMQFGGGPTVFQFGGGGRTTFRRAGFNRRQQQQQNPNEPEVRPASTWISLLPIILFFVVSLIQSFPSLFSSPSTPDPSFSWNPSTVYPIQRVTHTSPGVTYFVNPVEFASHPLWEQYLKANPGLSGTSETTNEEKTIEENKQTLIQDLINESRQLVEKRKANQRQYLVIPKEFKRFELEIENSWVRKLKYECQNARAHRDARRRELMGFLGIGSDWEAIKKLEAELIPACEGLKAMGYVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1S5</accession>
    <name>A0A5B0N1S5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N1S5"/>
    <dbReference type="OrthoDB" id="10585694at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="421" mass="46915" checksum="D13BE381AE41DAF4" modified="2019-11-13" version="1">MDPTQSSSSPVCSPDDHSREASDMKVRATMGELIRRLEGMQRRIDQKREGMEGRTEGEESLMDRLKTDLLPALDEGLGQLPGQLLVQPATRSPSRRGFRPASEIEAGLAIIVRTIGDTVEKIVRIVGRSTAALPARFLSSDDSSLPQEEIDMRAFRREEIRGQTTALIHRLLEAFKFLHYVASSTPIGFPGQRSPSEESSWLEEGGEMEEWVEMNEGVREAVCSMRGSMARSDLAVLQARWQAMAGMVERTLARLSYLAGQAGGQQSHQALLVRAAVPIVKLSRVFFHKLADPTSVFLEDIAPHQLLESLLPSTLQLADSVNRLDFSIKLLPPPPPNLPPLHISSSTPPQWLQNTAQSFLHTLLLLLHSFHNLSLNHLNHPPPLINSLQNSHLLWLLTCQTLVDRAFQNFLHVYLSTYNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3L7</accession>
    <name>A0A5B0N3L7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_011273</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3L7"/>
    <dbReference type="OrthoDB" id="78198at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48471">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48471:SF1">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="245"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1082708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="336" mass="38254" checksum="B6F5085A3074E7CD" modified="2019-11-13" version="1">MVNFDQLLLEQQQQSQITNQYQQSADEVMRRTVESFFNDTDQVQEDDKALLLFCALAYLGTPRQFRQLRTYLTRADLPCVPMAHSAWAYLWKSRSDRAFITTMGLDVSTFDDLLERFSARWNYTTIDRGDVNPMGEPQLGRRSLDAAGTLGMVLHWLCSTMSSYSLQQLFGVTPAVCSRYLASGMQHLLAVLQEHPQARFLWPTTDRRAQQYSAPIERKYPLLTKCFGFLDGLNLPVLVSDDEDCILAFAPDGTIMYAILNAPGSWHDATIAEPLYSQLLERTPPGYRIISDTAFPRKSERIQARILAPAKRGDRLPRDSVDFARLQLLNEQLVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4G2</accession>
    <name>A0A5B0N4G2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CPR2_1</name>
        <name evidence="7" type="synonym">CPR2_2</name>
        <name evidence="6" type="ORF">PGT21_023617</name>
        <name evidence="7" type="ORF">PGTUg99_021412</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4G2"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000001">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF569">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033905482" description="Peptidyl-prolyl cis-trans isomerase" evidence="4">
        <location>
            <begin position="26"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="39"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1083038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1124026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="252" mass="27319" checksum="E1A59FB607229CCD" modified="2019-11-13" version="1">MRSTTLLSWLLSAFVALVLHTTIASQQPRKGPVVVSKVYLDITRGDKPLGRIVIGLFKRTPKTSENFRVLALGNTKSASGTPLAYKGSSFHRIIKNFMIQGGDFTKGDGTGGESIYGSKFKDENFLYKHEGAGTLSMANAGPDTNGSQFFLCTVVTSWLDGKHVVFGKVIEGMDVVYDIESSKTDGRDRPIEKVTIADSGELEIEDEVDEEGNKFGVKGNGNLLASSSSSSSYIKTSSLKETVTWSREVDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4J8</accession>
    <name>A0A5B0N4J8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_019314</name>
        <name evidence="5" type="ORF">PGTUg99_003153</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000455">
        <property type="protein sequence ID" value="KAA1077130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4J8"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF5">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="4">
        <location>
            <begin position="269"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="4">
        <location>
            <begin position="485"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="135"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="416"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="608"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="672"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="799"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="608"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="644"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="676"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="799"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="810"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="824"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="859"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1077130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1084155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="929" mass="102062" checksum="36AF04525DA831F8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5T8</accession>
    <name>A0A5B0N5T8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_014431</name>
        <name evidence="3" type="ORF">PGTUg99_022929</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5T8"/>
    <dbReference type="OrthoDB" id="10634002at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366217" evidence="2">
        <location>
            <begin position="20"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1094235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="415" mass="41608" checksum="3844CAE4D8B61D16" modified="2019-11-13" version="1">MMIKAAWIIVAALFIGKSAELAIPAKSDASSYGQYKPDVPQNPSPSWPGKPGSGDSDTENGTTKDADSKAGSGAGSPPTPTGPTSGLEPPSTHMNGTIADADSQPAPDGASPAAGPTGPKGAFEPPSSQMNGTASDGDSQPAPISGVDNSPEQKNPNGSYQSPSNGINGTIADAQGKPGSGPDSGPDDHSLGAPSQNGTLSSPVDKQPGGDQSGDDQPSVGQPSGGQPAGGDQSSEDASGPGHPYVKRNPASTVSPYPAGKNSSDCDQPGPSPYQGSLNNGTQPALDDKDASSTTAPDEQPDHSGDKKPLPDHTPGSEEGDEDSDDEDSDEDEDENEDEDQDGEEDGSKKKGQSKDASEGSADDGPSSVPPQTPANGEVQSPSSKNPADDHPDSYRSLASPTDKHNKPAAPGAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6I7</accession>
    <name>A0A5B0N6I7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Putative RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DBP8_1</name>
        <name evidence="14" type="ORF">PGT21_023022</name>
        <name evidence="15" type="ORF">PGTUg99_021162</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6I7"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17955">
        <property type="entry name" value="DEADc_DDX49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="51"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="306"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1084276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1092335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="502" mass="55679" checksum="9C5E0E28F7F45C78" modified="2019-11-13" version="1">MDILNSSASNGLKTNLSKPASFASLGVQPDLVAALTAMSITSPSEIQQKAIPDILAGRDCIGSSQTGSGKTVAFAIPILQALAKDPISCFCLILTPTRELAFQIGSQLKVLGSLAVPGFTYSVIVGGMDMMSQALDLNKRPHIIIATPGRLVDHLKSAEGELGRWNLRRCKFLVLDEADRMLTPTFAESLSYLINEQLPPPTDRQTLLFTATITEPVLALSEKTPPAGKQKPVVHLCGPSRINLPATLKQFYSFIPSQVKDVYLYYLLLNVCDLTGLPPPPKQPKATKSKRVKYSNKTKRLINEDDEPDPVSNLPQTIIFVSKPRVAEQLHLLLESSHLPYPIKSRSLHSNLSQMQRLDAIREFEERQVPVLISTDLGSRGLDLKQVKLVINWNLPLDPNEYVHRVGRTARRNQGGEGDGVSISLVAEHDIECVLKVEEQIGSKLELIPLKEEKVLEYLNPISKAKREAELDLNEMKFGQRQLINKKKQQKLDKIRKAEQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N878</accession>
    <name>A0A5B0N878_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023442</name>
        <name evidence="2" type="ORF">PGTUg99_019265</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N878"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="909" mass="106116" checksum="7A9E761F1F1B6EB7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAP5</accession>
    <name>A0A5B0NAP5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3 10">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PGI1_1</name>
        <name evidence="12" type="synonym">PGI1_2</name>
        <name evidence="11" type="ORF">PGT21_019216</name>
        <name evidence="12" type="ORF">PGTUg99_034690</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="3 10"/>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NAP5"/>
    <dbReference type="OrthoDB" id="5831190at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000001">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000004">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1085783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1136190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="556" mass="61602" checksum="7AD57DE967BAF7EC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBM5</accession>
    <name>A0A5B0NBM5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBM5"/>
    <dbReference type="OrthoDB" id="2506824at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="314" mass="35872" checksum="AFC8BC7C541E47B0" modified="2019-11-13" version="1">MAKDCQCHRLFYPTLIRSRSSNYVINSLKTKTTISPVITTLRTIGVMQRKKRKNEDDLLLKSKLSKPSYNNTVNQFDSPIGKRTFPQERFESDYHQFKQAISISPESQAFRLVSNGFFVGLTYDALIRPKQGKLAKRITTQQPELSHLSSNSTTTFTNIQSHDYERRINRHTRNPDHHHHQSSHQMARENCGRTRKASFSVNNNPWTLRPIMSTPEQQSNSNPSPKIGISEEPGDREQLIFTMSPFSSTQTLSTFIRSSATLPESHRSLKAFGRRKASRADDLGDESEDDEDERSISITSVLPIDIPQSHHKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NCX4</accession>
    <name>A0A5B0NCX4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012332</name>
        <name evidence="2" type="ORF">PGTUg99_020088</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NCX4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1136000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="172" mass="18726" checksum="F62F74B2A0D57955" modified="2019-11-13" version="1">MHAACFVPHLKPIVSRLFHAPVKKSCILVVPWGIYGHYGDAATAAKLTPIPEETVLLNDPDGDRTRDILRSFPDPGRCKAGILPLNYGADSGMRKAPIPTAAPSPMALLTPQSMCYAVVCKTLTDLSHRDLDRASAVEVTCTSHGWYFKHFGVPARLIKTVDLGSNNHPTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDU6</accession>
    <name>A0A5B0NDU6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDU6"/>
    <dbReference type="OrthoDB" id="2508656at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022903848" evidence="1">
        <location>
            <begin position="20"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="610" mass="70426" checksum="04F3BEE13B630AAE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFR1</accession>
    <name>A0A5B0NFR1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFR1"/>
    <dbReference type="OrthoDB" id="2499476at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="356"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="696" mass="80970" checksum="DF2D2D66D8DA66FE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGI9</accession>
    <name>A0A5B0NGI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGI9"/>
    <dbReference type="OrthoDB" id="2508484at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="248" mass="27447" checksum="2BE388A9559B3F50" modified="2019-11-13" version="1">MRRGDILASFLGLLLCPSSRNLLRFSIAVPLEDHTRALTNVHSIVKRATPSQPSAPTDSVPDVLEKLAPEEDLVPDMPADLFPQDIASLQFAWNHLLEDDHHGVGLALPETEIDNQIGQLNADLVQALKGHDKPMSPEAITDELEHAPKETTRPKMSRQLPSAESFESGIEFNRLAQRRKMIPESSNSQADRHLSSTKISQVGPNTKILEPSTRSEQNLILDRPTPSWNPEPSESSGVTRGLKFPHFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGL9</accession>
    <name>A0A5B0NGL9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007709</name>
        <name evidence="2" type="ORF">PGTUg99_029234</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGL9"/>
    <dbReference type="OrthoDB" id="2506661at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1119"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="759"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1239" mass="133860" checksum="FB2CAA0A38193F45" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKX1</accession>
    <name>A0A5B0NKX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKX1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="10969" checksum="232416202CC5B222" modified="2019-11-13" version="1">MGQLPPPSTPKLLELVRAQTTLDAFVLANVLEGLVRAAKEAKDSTCSFQAYGFGLGTVAELAIEFLSKNRSSAVKESIERENNPIVAARTVTVNVQPYKSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NL05</accession>
    <name>A0A5B0NL05_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Complex I-ESSS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NADH-ubiquinone oxidoreductase ESSS subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGT21_012087</name>
        <name evidence="19" type="ORF">PGTUg99_006032</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
            <strain evidence="19 21">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Complex I is composed of 45 different subunits. Interacts with BCAP31.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB11 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000139">
        <property type="protein sequence ID" value="KAA1128728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NL05"/>
    <dbReference type="OrthoDB" id="2147978at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019329">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ESSS_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40637">
        <property type="entry name" value="ESSS SUBUNIT OF NADH:UBIQUINONE OXIDOREDUCTASE (COMPLEX I) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40637:SF1">
        <property type="entry name" value="ESSS SUBUNIT OF NADH:UBIQUINONE OXIDOREDUCTASE (COMPLEX I) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10183">
        <property type="entry name" value="ESSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1089266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1128728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="127" mass="14642" checksum="58EAE22097CE8DE0" modified="2019-11-13" version="1">MASHQIPRAFNKLAQQSTSLSRLASHPRLHRTSLLQQNVSGSLASSVRFGSGEPHYNQPSGWLFGEKPLKPGQKRVREDWELIWYIGFWGTTFVGIIMQIYKPDRSITTWARHEAEQKMEKPTYEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPF7</accession>
    <name>A0A5B0NPF7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011743</name>
        <name evidence="2" type="ORF">PGTUg99_010465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPF7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="86" mass="9381" checksum="A223D188CE3B8E52" modified="2019-11-13" version="1">MLIIPNPSVKTESASTFKTEHSERNRSKGIGSFKTTVQRGLACLKRTAQKDSSRSKGLVSFETDRSKGLGLFVVGSFEKSLSQDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQZ1</accession>
    <name>A0A5B0NQZ1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="3 8">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">DCW1_3</name>
        <name evidence="10" type="ORF">PGT21_018359</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="3 8"/>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQZ1"/>
    <dbReference type="OrthoDB" id="9984024at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF36">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016302">
        <property type="entry name" value="Man_a_manosd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022748010" description="Mannan endo-1,6-alpha-mannosidase" evidence="9">
        <location>
            <begin position="26"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR016302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1090934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="472" mass="50675" checksum="505DFAE77B55F016" modified="2019-11-13" version="1">MILNLRRLTRCLFLSSTLAPSFTQAVSLDVNDLSALKNAASTALKNLMSFYTPNPSGVFDQASTPWHESGMIWGLFMDYAKYTGDTQFLDVVTGALVNASYGAEQDLLGGAMSSTAATLLGRWNDDILWHSLVPVGGAEIYGAESIMPGSKGSWMTLAQKTYDQTYQQWDNGCGGGIYWSRDRNGASAPYKSLITQLEFIQQGARNFMENKNNTALDLSKAAMDWVFSSGLGNAQTGILYDGMNVGECGKFTTHLWSYNYGSLLGALAWMHKATGNNTYLDLIGPFYNYASQTFSGQNVSNVVTETCEPGARCNRDQQGFKAVYLRNLAYTYREASSDSIKNSIKTTVDASVAAMVQNSCDNEWFCNGNWTADASPVKYVRSQHVSAALLVSALGIHLNPEGGLLPKITPGQLVDTTNGAAGLGSGGTKNPGKVTSKPLAAAPGLSVTFSSLFFGLFIAAISSISPLDLIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NR01</accession>
    <name>A0A5B0NR01_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_005624</name>
        <name evidence="8" type="ORF">PGT21_007814</name>
        <name evidence="7" type="ORF">PGTUg99_022102</name>
        <name evidence="5" type="ORF">PGTUg99_030335</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000340">
        <property type="protein sequence ID" value="KAA1100556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NR01"/>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000011">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="106"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="183"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="222"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="262"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="294"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="301"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="340"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="373"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="379"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="418"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="496"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="579"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1074210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1090570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1100556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1110037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="622" mass="68075" checksum="BE0A676B62B1C650" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTU4</accession>
    <name>A0A5B0NTU4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTU4"/>
    <dbReference type="OrthoDB" id="2501212at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="167" mass="18316" checksum="D85FF40BCF4F6A74" modified="2019-11-13" version="1">MNEQGELLKSQGGQCYCQEAADQQVYLACEENQWPTIAQQPPDRPMMCAKGRSDEGKAVCPTGYSPAFLNLVKGYDLVAANRGTQQVSVCDKKTFTKVGSMEADLFRRSSGVDNTLYADVKGCSCQAGNDKQLQFKCKPWPTSLSVSYQENDPNLICVNGISCPKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZB5</accession>
    <name>A0A5B0NZB5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phenol hydroxylase-like C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_027719</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZB5"/>
    <dbReference type="OrthoDB" id="1716816at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02979">
        <property type="entry name" value="PHOX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012941">
        <property type="entry name" value="Phe_hydrox_C_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038220">
        <property type="entry name" value="PHOX_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050641">
        <property type="entry name" value="RIFMO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004:SF20">
        <property type="entry name" value="2-MONOOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G13660)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07976">
        <property type="entry name" value="Phe_hydrox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phenol hydroxylase-like C-terminal dimerisation" evidence="4">
        <location>
            <begin position="43"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1094657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="259" mass="28825" checksum="292E827B6EA21131" modified="2019-11-13" version="1">MYQFARLFSGKPAKDVLDESGISMEEFQRTVQKGNIFSSGTSVDYPSSSLVSKNERRQIANELASKLPIGPRLYSRQVVGVADAKPYMLGDLACADGRWKILLFGGDVKKYSGCRLRLEKLCGFLANDPASPIIKYTPKDANLDSVFNFLTILASPRVQLECEDFHDILRPKLGKTGFQTYKKIFSDDESYHRGHGKIYENYGIDPKVGCMVVVRPDQYVSLVTEIEDHNGLASFFDSFMLPAGNSSSSFQAPISQSTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZU9</accession>
    <name>A0A5B0NZU9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_92</name>
        <name evidence="2" type="ORF">PGT21_029889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZU9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="726"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="726"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="757"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="797"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="818"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="861" mass="95450" checksum="70E1521B070E4F3A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1H7</accession>
    <name>A0A5B0P1H7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4042 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002291</name>
        <name evidence="4" type="ORF">PGTUg99_007128</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000115">
        <property type="protein sequence ID" value="KAA1129987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1H7"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025283">
        <property type="entry name" value="DUF4042"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052107">
        <property type="entry name" value="HEAT6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13366:SF0">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13366">
        <property type="entry name" value="MALARIA ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13251">
        <property type="entry name" value="DUF4042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4042" evidence="2">
        <location>
            <begin position="209"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1129987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="790" mass="87733" checksum="72CE950C8C6C2473" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3C6</accession>
    <name>A0A5B0P3C6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024572</name>
        <name evidence="3" type="ORF">PGTUg99_025399</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3C6"/>
    <dbReference type="OrthoDB" id="5392716at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="194" mass="21817" checksum="01E4F74CD7ED87D2" modified="2019-11-13" version="1">MNQDITTSEYSDNTGPDDPPSPITTITPTPLSDNQKHDIITSLLLEGWKGPDVLMILNEEYGWSISLCTLSHLRQSWGLHLSDLPPAENPPDLLPPIRASLISAHQNGLTVAEMRSQLSSELGVNVSHCTVERYLRRLNLKQRQNDLVNGKTTREEVVELVRHARDALLANTAGYRRMRQILVTNYGLRLPRYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5P1</accession>
    <name>A0A5B0P5P1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005163</name>
        <name evidence="3" type="ORF">PGT21_005206</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5P1"/>
    <dbReference type="OrthoDB" id="5562676at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018786">
        <property type="entry name" value="Mit_KHE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062">
        <property type="entry name" value="K+-H+ EXCHANGE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10173">
        <property type="entry name" value="Mit_KHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="145" mass="16489" checksum="DE7FD82EBB5CCF94" modified="2019-11-13" version="1">MLLTKPSIRLIALPLSKRSNTLIYFYAQQLPSLADQKSSETSLPRHASFNLSPSSLIKKMTDTGTKQWNKLANSAHGSWKLKIYNTGERMMDKIPFEEWALKSIDPPHIPHKILPSESHNEKNQTGLKEAQKTSQVALYVWNENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P6R8</accession>
    <name>A0A5B0P6R8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell morphoproteinsis protein PAG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TAO3_8</name>
        <name evidence="2" type="ORF">PGT21_013144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P6R8"/>
    <dbReference type="OrthoDB" id="6287725at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025481">
        <property type="entry name" value="Cell_Morphogen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF30">
        <property type="entry name" value="PROTEIN FURRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14225">
        <property type="entry name" value="MOR2-PAG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein C-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="187" mass="21230" checksum="773E3F99048A2EAE" modified="2019-11-13" version="1">MQYEIDEIALSVARIAETFRMDGLSRVMVSFAKNQFRTKEDFLVLRQAVNGIREYFLPKFSADILIFYLGLLCNPLEWLRLKTLAVLKLFLKTIEPTFSSSGINELGYDLLTPLLHLLQTTASQQALDILAEPMPIKSRPLNGRKAMGTTSEEGSSKKVFGIPDETGWCVPNPQESMRVTYLHEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8C6</accession>
    <name>A0A5B0P8C6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_050176</name>
        <name evidence="2" type="ORF">PGTUg99_050124</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000173">
        <property type="protein sequence ID" value="KAA1125973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8C6"/>
    <dbReference type="OrthoDB" id="2513458at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1125973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="109" mass="11668" checksum="5B3D4FC5C9892586" modified="2019-11-13" version="1">MSVSIKAFTPIQLLWLKDMQLWVDKILEEVEPAPTKESPCPDASGWEGHIITQGKINSGAPDLRIPLSAVPNVMACWNARRNRLGRGPIVPIGIPDAGSSGHAGGAAQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFN1</accession>
    <name>A0A5B0PFN1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_014724</name>
        <name evidence="7" type="ORF">PGT21_019136</name>
        <name evidence="5" type="ORF">PGTUg99_012228</name>
        <name evidence="8" type="ORF">PGTUg99_031319</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the THOC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000004">
        <property type="protein sequence ID" value="KAA1138110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFN1"/>
    <dbReference type="OMA" id="WNADGRH"/>
    <dbReference type="OrthoDB" id="340259at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25174">
        <property type="entry name" value="Beta-prop_THOC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="11"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="56"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="201"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1095276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1099612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1117670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1138110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="322" mass="35361" checksum="733C5E4B02275070" modified="2019-11-13" version="1">MALKPPATRLLTGHTGPVFTLGWSVDGRKLASGSKDEGIRIWNPERADYGKASAELKGHSSRINQLRWDPTHPERLASAGEDGTLRFWDIRQGSKPIRTVENDSKLSFLNLCWSPDASIVAIGDSKDRLQFYDSSTGKKLAEHVEFSRSPNASEGQKDQINEVSFSHSGDYLFVPTGAGSVVTYAFSREGDAAKLTEFHRYEAHACVINCIDIDPRGRYIAVGGADSIVSLIDLEEFYCVNTFAHLESDVRTISFSYDGDFIAVAGKDFFIDVSAVSTGQQVRKIDNLTYPVNSIAWHPSKYVLAYAGDEKAGNVRVANLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFY0</accession>
    <name>A0A5B0PFY0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030286</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFY0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1100141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="185" mass="20470" checksum="34948B5FDBA81E59" modified="2019-11-13" version="1">MCRIHSRANYGHCDRSLRVRATPCPVPRLCAGIHTDDRFNFSLVNRTCLMNVPPGATYAIRHRIGVRPTSLLCYVKLSLFECCASAVYIKASHPSALLHPTNPSAKQSSFEPQKKSEQMRFILGLVIMVLSYVDFPVQSINNHNSAPCPEKTSRKGCPGPSTDPEIICSCCFDGYGRNVGYKTCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PG84</accession>
    <name>A0A5B0PG84_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PG84"/>
    <dbReference type="OrthoDB" id="10391579at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022811085" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="31"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="99" mass="11030" checksum="2208100D787A02AE" modified="2019-11-13" version="1">MQFHASRYLLVAGSLFISQVMSQVILETCGKNECTGKLEIISQQFGTCEKIFKCDAGEYHDECGTGTVLSYKKCNQCNRSVDEEKCIAAPHDFGPCEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHS1</accession>
    <name>A0A5B0PHS1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHS1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="85" mass="9569" checksum="5FA780CE3FEFD6A4" modified="2019-11-13" version="1">MAREGYSGGLDIARTVTRSDCPYHQHSPEPDKKQQRELTGKEDIQTGSWVKLIAFLVISSQQPHGHEGEQHTVHHLTLPTSAHFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHV5</accession>
    <name>A0A5B0PHV5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione S-transferase, nitrogen catabolite repression regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">URE2_1</name>
        <name evidence="4" type="ORF">PGT21_030982</name>
        <name evidence="5" type="ORF">PGTUg99_018377</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHV5"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01151">
        <property type="entry name" value="Main.2:_Nu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="109"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1100174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1120657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="241" mass="27642" checksum="031BE13EA0FB0886" modified="2019-11-13" version="1">MISKSEQKDLHLLTMSTPNGKKVQIALEELKLVYDDISFSHEMIDIRTNQQKSEEFLKVNPNGRIPALIDNKCKALNDPSAPFTVMESAAILLYLAKKVDKNHVFGFEDDLERSEALQWLFFGMAGVGPMQGQLNHFTRYAPEKLPYAMKRYHDETLRLYGVLEIQLSGKYTGVKKKYLAGKGEGKYSWADMAIWPWVDIYTISGITDEEMNQFPCLMEWLKSIRERPGVKEGCSSKYDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PK69</accession>
    <name>A0A5B0PK69_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_020552</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PK69"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47251">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04180)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47251:SF1">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04180)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="G-patch" evidence="4">
        <location>
            <begin position="86"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="184"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="210"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="433"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="377"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="406"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="446"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1101456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="50594" checksum="EC754B41E71E5F22" modified="2019-11-13" version="1">MSSLDHLSRLAQRYQLKRTRSISSGSSSSSSSASSSGYGSILSADEDGKKNDQSDPQSKQNEPGSHPPKQNHSNHSSDHQGDRLGIDNKGMQLLLKMGWIQGNGLGVGQKGRKEPIPTPAQTPLLGLGKATQDAYMLSNSISKPKELESMVIARETEEAKVKREEKVKEIQTRVVEREDRLKTFHCQVCDKGYTTISQFEEHERSYAHHHARRALEAKEARKSMGSSAEAKLEKERKREAKELERMARAAGMSLDARPSVSVKPELPKTGSAGFKKPTAGWIKLPPPASTLSTSQNPDPPIDSGLGILPADNSKPINSFKPSGFKKSSAFAPISSASSSGFKPIAGTSTANRPNHLTSSSSSSSSSATPTRLPPTFVASSSIETSFKPSLNHPPEIPISIDDRSNSDTTGASTPAQPTSKFAQIAARLAARKAAAAAAAPGLPSHADTTGSSMDKPNSKTATGYDSEVEKMLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PK90</accession>
    <name>A0A5B0PK90_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PK90"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="57" mass="6205" checksum="A6F5B038359BD1E2" modified="2019-11-13" version="1">MVFIYIFAVIQLITNTKSRISSRDNSLPEEGMPVGKISDEVPRGWDNGVSAANPIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKJ0</accession>
    <name>A0A5B0PKJ0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKJ0"/>
    <dbReference type="OrthoDB" id="2505003at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="51804" checksum="119C23FF3CAB5961" modified="2019-11-13" version="1">MKKQKKKTTTQVNDSDSGSIIDLREDSEPDNSKIPAAKPCAEKYDSPLEFFGEPFYPDKTTEEERKKKPITYNCKWCKKRVRGGHNSDANLQKHRDGSNQAGRDGSGCPNRSLAIQAGAKLPPTVNETKAQAEKNGTGDKKLTSFFGRTEKFDKKILNQNLMIWQTRNALPWSRIEDIDLQAGFHYCQPDAILFKRKWVAAEAKQLYISLQGAMLQTLKKSSSKFTLVHDAWTTKGNRYAFIGSSVAYINDEWEFNMMHLSLKLVSWFHQGKWLAEPLANVLQKHGIYPKISHKPFFTTDSASSNNTMATRVYDILADKNGTYSFDWRPERMHIRCFCHKIALIVNAGLAELGMIAPPPAKIKESVLGTFPYSDAMETITEVEEEMEEDNNGPASCSVGEDNINIDDDSEPEHDEEELSKLLEQQEKDAGNLDEEQREHQATNRNESNSLDRLTKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKU3</accession>
    <name>A0A5B0PKU3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018898</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKU3"/>
    <dbReference type="OrthoDB" id="2504239at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="155" mass="17340" checksum="3178890B9F776730" modified="2019-11-13" version="1">MVWERLKAAQKSGDTFLSKILLATYNSLETNPDSQPVITHATSVFAALLPPDREALTKDSPQAADPNLAETEIEDNLVYAVGMVMSHQYIGFTPYFDENIKKLQAPLLLTIFDREWQKKALNAHLLLKPAKSSEDKAYRGLAFRQMDSVSLGLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PR69</accession>
    <name>A0A5B0PR69_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PR69"/>
    <dbReference type="OrthoDB" id="2511830at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="339" mass="36298" checksum="612E26D86EAB61A0" modified="2019-11-13" version="1">MECSVFQNPFQLRRSSSQLSILTGTPHPSTVVTTAAARSATMPSGHYPQSLEQHISSPQPPMDGKTSPGGLKPLLLPTAFSPQLPSTPELGPFSHHHHHHHHHHHHYPRKSSAPSPSWVRQSSFSASIAPASSCCPSRQSHYRQTSVSSSNWPSSSSSSSSVSSASSISSYHNPTLDCLQESSPTPQPGRSTRSLSANYATHVLPGSSVIRTSSLSHALTPINPSEPGTRRAIHSKRSSISYSKFSPPSFLPHPSISPASTVYSSEPVVFSANPTETNTPTESDTPQSASPSLDPSQPESIDKLLDELLVELVQCAHSLGLQDELVGIMFIASFSDKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUH7</accession>
    <name>A0A5B0PUH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUH7"/>
    <dbReference type="OrthoDB" id="10305832at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="305" mass="33976" checksum="D54E9F1F7F96CB8A" modified="2019-11-13" version="1">MPSNKRNSNQVQLSSLSPPRIRPCLSHAQDGPSSSEFTAQSKLNQSPSEGQQSTAVNDVIDGSGTRHVPIARDHLGASQASGSGSKVVQSSARIDVDTSETDSLLAIEKTQSAKSPAYGYFLPYGQVVEELLNKHSIAFRKQIKYVQARASLGDAATSKPSSRLSRKNRIGGGRIAMRSDSLKDPMPKTEELAWLYNRLATLMYKLHVEFLSLFDVSTFDHRIQQERLLDWLDKQIVNPDNRQSVLKLMKETELNRASDDPETNIDDIQLKLIKYFSQERSGAHLILHHTASHLLNAFRDKHGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUS0</accession>
    <name>A0A5B0PUS0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Srp40 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUS0"/>
    <dbReference type="OrthoDB" id="5599646at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039191">
        <property type="entry name" value="Nopp140-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007718">
        <property type="entry name" value="Srp40_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216:SF1">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05022">
        <property type="entry name" value="SRP40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Srp40 C-terminal" evidence="2">
        <location>
            <begin position="441"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="318"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="514" mass="52692" checksum="FC3A55B6E6C136D2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PY50</accession>
    <name>A0A5B0PY50_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1105767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PY50"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="79" mass="7665" checksum="BAB93E8FA4AB2656" modified="2019-11-13" version="1">MRIPRTNLTPAPSVTPGLAPPNTPMDLDDGVGAGDQANALKDKDVPKNAAGHVAVDGAEVSTNGAGASALGTGESGNMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q379</accession>
    <name>A0A5B0Q379_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="128" mass="14770" checksum="BB463B777E667221" modified="2019-11-13" version="1">MEENLGPEAIQALEVLDQHKRACQERYYRQALKRESEKARYVDTHSKVNSLKQMVAKELGFKVTVQHPRLWYLLDTEVGVPIQNLGTPPTPRWNAQGQLGRGDKPLLLLFFFCLSLALLFFVIFANQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5A0</accession>
    <name>A0A5B0Q5A0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1108273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="63" mass="7039" checksum="26E1CAA45B83A6DA" modified="2019-11-13" version="1">MHPEITYEIARDGRAASGYPFGFGYPLAISAESDIRIRIRIRWRIPAGAGGYPRGYPRISAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5Q3</accession>
    <name>A0A5B0Q5Q3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent DNA helicase sgs1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SGS1_47</name>
        <name evidence="2" type="ORF">PGT21_015835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2507066at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="12823" checksum="6EA224ADAEA33DBB" modified="2019-11-13" version="1">MLFDHITLFQSTDSYQYISHRQEELDAELDSKAARLRKEKEDKDNQRAEATRAIQEAAAQKKIDTAARVKANAEKKRTAKEAKFIESEKKKAKRESDMAFLHQLQAQAEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5V1</accession>
    <name>A0A5B0Q5V1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016557</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5V1"/>
    <dbReference type="OrthoDB" id="2287304at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="109" mass="12479" checksum="EADE9F261953FC82" modified="2019-11-13" version="1">MDHSEDNNTPVDVLPQEVLAQILIRRQHLASSSNARRTMLLESATLLLDDETGYDDLIGKHGGSKPGKKPNIPRNFEAGYQQLYQHYFSESPLYDSRLFRRCGTTLWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5X1</accession>
    <name>A0A5B0Q5X1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5X1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="127" mass="14193" checksum="FE3134B03ACC266E" modified="2019-11-13" version="1">MKYVSDHVSLPSHKEAVRRYFAIVENLTPTEVSHTLGPNTNQPAPRGHDHPRSHDMYKGGDPGFTFYLPEKMPQGPSEEEGFTFDLPEEMPQGPSEEETPIRDGEALGFGSDSSEIEEAPSDYPEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6D3</accession>
    <name>A0A5B0Q6D3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020946</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6D3"/>
    <dbReference type="OrthoDB" id="3237105at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="123"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="942"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="973" mass="111895" checksum="3B95DC6FEB99BB88" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAB6</accession>
    <name>A0A5B0QAB6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAB6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="93" mass="10459" checksum="43E538F7DBB62786" modified="2019-11-13" version="1">MLSRSKTCGSVRPKLLSWNSPLQGGYVSSSWPPSCSRLVFARRIRRDNVTTLIGSLLGHLIALAESSSLGVYPIRRDSDLDDDICSFRMFHFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC04</accession>
    <name>A0A5B0QC04_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QC04"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="189" mass="20654" checksum="C5E4FB685F290625" modified="2019-11-13" version="1">MFLLQVPLLVITFAIIGPIFSIPMGHEMAGEGSASREADGKCPPGPQTYQVIHPNLTPNGASTSWEIHIHIGDLVGRTEEDPVRLNSHLEPEKLALSQSSISIADENIAHGITYANLPDAVNQAPKTSRERQEVLFRLNVAAVLPAFIGFGFIFTNINTCDPMLSFMWALFGSLYIYAANYLATKIAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCF7</accession>
    <name>A0A5B0QCF7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032536</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCF7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="146" mass="16317" checksum="3CD100C17A983F32" modified="2019-11-13" version="1">MLQPKQQSNHPPQASSSSALHHNEAARHDTHSKELAQLSTQLISAGFLRQPLPPDTLNGGPLEGQKYLIKAIWSMIASCSEMLIREGLLAKQRDLSYEHTRLEVFLERAEKEKLEAQQEATASTNRAKLSIEMSSCQIISIENLGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDJ1</accession>
    <name>A0A5B0QDJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Eukaryotic elongation factor-2 kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">EEF2K_34</name>
        <name evidence="2" type="ORF">PGT21_036960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QDJ1"/>
    <dbReference type="OrthoDB" id="10658352at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903013">
        <property type="term" value="P:response to differentiation-inducing factor 1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF2">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="619" mass="69194" checksum="EDBBCDEF7B6F51F0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEM5</accession>
    <name>A0A5B0QEM5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022050</name>
        <name evidence="3" type="ORF">PGTUg99_006273</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000283">
        <property type="protein sequence ID" value="KAA1111434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEM5"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF6">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="116"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1111434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="463" mass="52333" checksum="22676CA1B0785DC1" modified="2019-11-13" version="1">MPTSLLSMAPKVQESQEAEACPFKSRSYVYDYTKVTELPIAPTDENPSRDGFTKQRCYPGSDPEAEEYCVYANPSFGGDRGMILYIRPSYLLQKLDLFTEFKPDSALITDKERTEFPFKLVHMPSKGGLGAVADRKINVGQLVIVDYSLVYLSADITEIDLSEWAPRLKYMVNLLPLKGRELFAKQHGVGEIDTAWIVSAFQRNVFAAPQDNDSQGGCAFAPEPAVSFLFFGGGGIKAQDDSKHLLTDMISRSCLMLYMQYLNHDCRPNVGYRFNNVTIQVEMHALREIAPGEELSISYITLVQSREKRRKSLHGTYGFHCGCSQCSLSDAESEASDQRVEKIRELWDVISDWDSSPPSTPAMADEILELFKAERMDVVMEEPYTMASLVYNSWGLTHQARQFSALAISYGVYTHDKTWLETSSHLPLIYDPESHWSYNIGKKMDAEVQTTQTDIAHYFHVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEZ6</accession>
    <name>A0A5B0QEZ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEZ6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="52" mass="5818" checksum="F3EA3C0D6848452B" modified="2019-11-13" version="1">MRSCASFRSFCFNSKIVYGALGALSKSRPQTSTINPEEKDRVFLSSINDNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHY8</accession>
    <name>A0A5B0QHY8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHY8"/>
    <dbReference type="OrthoDB" id="10529866at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="99" mass="11125" checksum="F003B51ABF497954" modified="2019-11-13" version="1">MALSIQAQGRWSCWQSDGEGRHARLHALCGASKDPLDKRSYLLGSDLSPGARVSRRHPARGRTKYQGQHTRRTPLVELLRLCSWPDWIATEAGRRCGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN90</accession>
    <name>A0A5B0QN90_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN90"/>
    <dbReference type="OrthoDB" id="10291282at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="12239" checksum="99B7390BC6A189EF" modified="2019-11-13" version="1">MGRPSHLIVTGAVTSGCAHVEVLGPHRHGDSPSECHQDELVSTQSSSRNEGAPATGIALRPARVACQEHLVTKAPYRAPRSTWGLWRHWGDLTFIDSPRCVPVGTVQICIHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNH8</accession>
    <name>A0A5B0QNH8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_008863</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNH8"/>
    <dbReference type="OrthoDB" id="2500329at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF9">
        <property type="entry name" value="AMINO-ACID PERMEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1114434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="40273" checksum="D449C2AF71397B9E" modified="2019-11-13" version="1">MCMTIAASVAELVSAFPTSGGLYSASAFLVPKRFKAPVGFLVGWLSILGQIAAVASAEFALSQMIWSAYTISQDGNYSPTKLEIVGVFGILLLIHGLMNSVATRIMAKLTRTFIFFNFGGTFAIILALCLSGPPKQSFEYVFTKIVNRTGWDSTPLAFMMGILSSEWTLSDYDATAHISEEIKNPAVAAPLAIMTAISVSGVLGWFLNLVLVLYSPDIASLTTPSSSQSNDVGLLRNASDVASAFANTTSISTSDASASALPVAAAAAASSPSSSSSSSDFLLVATILSQNLSTGLFYFTWTLICINAFFQVNVVLQACSRTLFAFSRDGGLPDRQFFGQLSKRTKIPFRSVWVVILISLFFGSLDFVSTVAVNAVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQS5</accession>
    <name>A0A5B0QQS5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_037048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQS5"/>
    <dbReference type="OrthoDB" id="3255642at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023018190" evidence="2">
        <location>
            <begin position="21"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="388" mass="41550" checksum="D4982ED915666FFF" modified="2019-11-13" version="1">MLKSLLVAFFFLLQLGTSFATGCGDECMVSVAKAFAGNYSQQVAPIFQGISRKVQARGLLPQLLTSQVDKLFAPSQQELYDQVYNQTLSQLVTGVLGNDNVTHALVEASKPLIAIVLNLAKSTLREAISIIEATTSRLEAEGKPSPTSYSNPSTLPESTVVGRPTALSTIPLRPNGGPKPPFRLRRRVLNSFGSDSSGQLVPRANPPPGTPVTVSTIPVAKTYEPLEAPKNVTPSTPTTTPATPTADRKCTTTLKRKIHVCIPDVLPILNSLRKRSTVLAEKLSTSTLPSLSFPKYIAGMALSSGRKIITFVQPLLTSSQSAGDLVFESEGLAASDQISLGSSEIVEGHPSTLLEDTLEISLDEMISSFETHLEDFHQTLVEGPLRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTU2</accession>
    <name>A0A5B0QTU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020597</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTU2"/>
    <dbReference type="OrthoDB" id="10320015at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="227" mass="24742" checksum="577934621CE58FB5" modified="2019-11-13" version="1">MYCNLTDLPVSCLDAQSQDWQDGSSAWYTTNPSIHNAIYANALGRVNFYPNTEWWTPKTDAKEVLAGSTNESFKFPQLISYAILRHQCTIPDPAPPICRIFSDCLWGELPKSGALFSLMAALEGFVKADATVFSCPRSNSEGWCGTLLGGDGNLTQSVGLLFCPVDVAAEVGHSPKYGPPSTVTNFQCIRNPQSMWCCPLHSLTFTPGTNQVSHAQITSTCQLHKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTX1</accession>
    <name>A0A5B0QTX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003496</name>
        <name evidence="3" type="ORF">PGTUg99_021829</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000109">
        <property type="protein sequence ID" value="KAA1130456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTX1"/>
    <dbReference type="OrthoDB" id="2148946at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052945">
        <property type="entry name" value="Mitotic_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628:SF1">
        <property type="entry name" value="CHITIN SYNTHASE REGULATORY FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="455" mass="50771" checksum="F9548D019AC73EC2" modified="2019-11-13" version="1">MEFKSEKPATLRKSASAYLRRKLSSKAPSRATTLPVESSTSKTSKDTCVSMLHPTKLCENPRIILHNVAEYFSKGLELNFSSDDASIESTPSTSSRTQSPVENTNPSLSQRSPSLCQGKSRHFQSQTWPPATRPILGRSKSEPAHESKPTPLNSYPGRYSSHSKLDLSRCENSMTTIKFSSTPNSQIHSPKPSPLSKEIVCEIEEVDPLPRAAEAGSSRAIPRSQEKENDLNPRLASKIKSKCEKSIPLGDSSEKQILHKGIVAHQKNDMTLSAILFERCAKENGGCGVGMLMWALALRHGWGCPVDTLKAFGWLQLSIETLLRELHHAKLEKQHYDELHSNEGTPKDLLLAQYLINETNGIKSELLLALYELGQCLMYGWGCPRDKRLAVQYYHLAATLGDADAQKELAYCYEVGNGVKRSSRNAAKYYRMACAQGIKMMGYQWIWKDKYDPVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QY38</accession>
    <name>A0A5B0QY38_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TAF13_2</name>
        <name evidence="9" type="synonym">TAF13_1</name>
        <name evidence="9" type="ORF">PGT21_020451</name>
        <name evidence="10" type="ORF">PGT21_023754</name>
        <name evidence="11" type="ORF">PGTUg99_015579</name>
        <name evidence="8" type="ORF">PGTUg99_031813</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="8 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAF13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000004">
        <property type="protein sequence ID" value="KAA1137965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QY38"/>
    <dbReference type="OrthoDB" id="10266074at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000143">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF5">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="154"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1095451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1099787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1117805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1137965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="182" mass="20640" checksum="4DB17642F08557E1" modified="2019-11-13" version="1">MSHQQSFSGPSHNDHNTQARSRPRISKKGLFAKDLTAMMFGFGDTDPQRDVVNLMEEIVIDHISDVLITAHRRSTNRGKLKVDDIKSALEESSTRVHNPTTLHGSTSRDLIKYTPYPLARRKVSLAEKQLSRIEELLFMQDDLARARGRADDLKAYGADDSPDDHHPMDNIHLTDKGKQREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0M6</accession>
    <name>A0A5B0R0M6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004394</name>
        <name evidence="5" type="ORF">PGT21_015148</name>
        <name evidence="3" type="ORF">PGT21_024562</name>
        <name evidence="1" type="ORF">PGT21_026982</name>
        <name evidence="4" type="ORF">PGT21_032780</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0M6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1113507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1119101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="138" mass="14821" checksum="12426D6BDE020786" modified="2019-11-13" version="1">MRLTFLNSHCCKYTLQNGHSEQRLKNIVPLAPALPFGTAIEERRSVALASLMLFRAVVEECPLGLSDADQNGHRRDCSLNDGRLERPSSTRGLGAGMYPVCRSGTRLPANLCNAGMPSGCAAMEAEGALLASSDELIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R253</accession>
    <name>A0A5B0R253_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R253"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="427"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="479" mass="49976" checksum="BFA5C3630DFEF662" modified="2019-11-13" version="1">MTELMELDGMAGTYIRVLSNLRSFYVSKYITRFRAVHPQIPLPNWIVTLASPGDLRPSDTQVRVTNMRADLDGETNRPPSPQQGNVISNEQEAMDEDPPRNDDNPLLPQAPEMTDIRPPQEDVVPVQGPDTTKDAIHHDKEMDLTSSDAEGEVDPEISFDVGNGHVAQSGKTKGATDGSNTEGTNMDKPLFLPESNPPSEDDSPLWGSNPKVGKSNRSCSPLSSPPSASLSEPDNPQPSPPAPKNPPLGLGNVVTLGQKRPITRSLTGDATRKHRPCLDSPSLASSDSDDSSSESEEEESQGGKSDLTHPPPLPTFGHIRQPRDQTPADSDEVDTDGPNSPKPLPKTAVVTTPNPTPATTPLVPAPTSSNTVNAGAPNHRPAGTGTTLGGGGPDLTARTTSTGPTAPTEGMTPTGPSRGPNSPPLNSPTTNSGGTPGTAAKHPTPGTLGTDPTSRQVPLREPSPGGPVCPAACGGSQMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3B3</accession>
    <name>A0A5B0R3B3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033624</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3B3"/>
    <dbReference type="OrthoDB" id="284184at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="21744" checksum="506063057AEEB4DD" modified="2019-11-13" version="1">MSPEFSEEIHRYRRTIILLLYLHTDRGLENLIQERFARLKDHSFVGENVLRKQVREAAPQLEKIEVKDPEIRYFLSEFEKGGLLGPLSWYKTRDIDHSDEQAASLPASYPAEIPCLYLGASDDPAFPPPLFTEKSKAKLFPGANLTSHIIRGGNHFMHQDPVYRDQVTEVIGNWIDNQLTTAQNRPSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3P9</accession>
    <name>A0A5B0R3P9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PIG-P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_037112</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3P9"/>
    <dbReference type="OrthoDB" id="690928at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="7">
        <location>
            <begin position="65"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1120070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="232" mass="25667" checksum="7D5B4A4955D4E9EF" modified="2019-11-13" version="1">MPAKEEEARIKESERDSSSRNKGASPTTQDSRSTELEPEERGGGTGRRKRQMKLPAPGTNALATSAFATYVLSTIIFTIYLLHGLLPSKTVEEVLGLSYFPAQVWTTILPGWLTIAIWYLFLAYFALNLSRTPALDGPDGLAAITDRAALTLLPPPSTRAPPPPRSSPHPTPHPSADSSDLPVPILRDLPFKHISPETHPIRHHHYHDWSQYHSISCQASLLVLEIFNSHQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R738</accession>
    <name>A0A5B0R738_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028854</name>
        <name evidence="3" type="ORF">PGTUg99_032658</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R738"/>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF3">
        <property type="entry name" value="F-BOX PROTEIN POF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="655"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="606"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="662"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="681" mass="76159" checksum="41558EBAC4B04A26" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RLT1</accession>
    <name>A0A5B0RLT1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Short-chain dehydrogenase/reductase 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Retinal short-chain dehydrogenase/reductase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_016870</name>
        <name evidence="14" type="ORF">PGTUg99_030302</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RLT1"/>
    <dbReference type="OrthoDB" id="10253736at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05339">
        <property type="entry name" value="17beta-HSDXI-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000131">
        <property type="entry name" value="Short-chain dehydrogenase/reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF736">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1076725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1126891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="364" mass="42027" checksum="D03F83D40E60B1C1" modified="2019-11-13" version="1">MDFAELKHTLRSINIDVLYSVTERTFLSPFFACWIPLLALGQSAPRFYYWSLRFLWFVLFRTALCWSSQMWRNRRWRRGQIDWNDQVVLVTGGSEGLGRVLVETLLLKHISVIVLDIKPFSDRDEEEEGDLKFYQCDVSDPQAVEKAAIQIRKDVGSPTIIVNNAGIVHGKSLLELEPDELQKTFAVNVFAHFYLYKAFLPDMIKRNSGHIVTMASILGHVGVSRVADYCASKAAAILLHQSLREELHSVHHAHGIHTTLVCPGLMNTKMFENVKTWNEFMFPRLSPHELMKKIIQTIDNEDSTEIYMPLYTKFNFIFDLSEFFLVPSWFLSFLHWFVGSNRAYNPHLSDPATVDPSVTHQKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RSQ8</accession>
    <name>A0A5B0RSQ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029102</name>
        <name evidence="2" type="ORF">PGTUg99_006744</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000142">
        <property type="protein sequence ID" value="KAA1128318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RSQ8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1128318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="64" mass="7342" checksum="0867C523DB8C852B" modified="2019-11-13" version="1">MYTTGAVLEMALRRLDPALQVEEQQHRRWPGLKAKSVEPTALSSSSWLDAPCKFMRRVSINLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RUP1</accession>
    <name>A0A5B0RUP1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026270</name>
        <name evidence="3" type="ORF">PGTUg99_018883</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1128989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RUP1"/>
    <dbReference type="OrthoDB" id="2507748at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="597" mass="67408" checksum="D7D7A35E0310966E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RZH5</accession>
    <name>A0A5B0RZH5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">GPI12_1</name>
        <name evidence="5" type="synonym">GPI12_4</name>
        <name evidence="5" type="ORF">PGT21_026946</name>
        <name evidence="6" type="ORF">PGTUg99_015646</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RZH5"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1080984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1131140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="402" mass="45832" checksum="2A3C71935EBE6D19" modified="2019-11-13" version="1">MSASQSKNRTNQEEEDIPPISPLLEPSYQYPSRDNQIREFDYGDREDVLAEAEELDSTNSHYPPSSKRSANNNNHHHNRTTTKTIINALSLGLRKHRTKFRLLVVFLTLWPLLCWTGIFLLFSDHPALFPSSLRGSKSTMFVVAHPDDECLFFAPSILATVQRAKSHGALLVMSSGNHYGQGGQRRKELLGSCKQLGIREDRCDVLDISQIQDDPTIWWPVDRIGKLVNEHIERWMIDSIVTFDDYGVSGHINHRAVSASVTELAISLYKKSVAPNHSSTKSPKLYRVKSVFVLRKYIGLFDLPLSFIRLIPSIIFGPSQQINSALQSLTYDPKTEQTTQESERQRSAVTATFEKGLLISGLSGYRSARNAFWSHQSQMVWDRHLYMILSQFMYFNTIERIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S114</accession>
    <name>A0A5B0S114_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014560</name>
        <name evidence="3" type="ORF">PGTUg99_031457</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S114"/>
    <dbReference type="OrthoDB" id="2495163at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="28163" checksum="4405C7990CAE95D6" modified="2019-11-13" version="1">MPRTPTLVEHVYFTGDRKEFDRFIYHIKYALSEQDKGYEYNGHSNGSEKMEINWIARHFRYPNGQNSMEEGPLSYNWWIGLLEKNARKQNLPISHAVSPADNYKIPELATKKAFLEHLHEAFGSPDAREVTRKALYACKQGDQDIQQFNLVFNTLVYAVDLTENERCDIYEERLNVDLLTTAIKHTGWRNAKTLHDKQALARSAAFILHKLAQLDLEKQNAENRDNQQPNILQHANRQPLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1D9</accession>
    <name>A0A5B0S1D9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026647</name>
        <name evidence="3" type="ORF">PGTUg99_007109</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1D9"/>
    <dbReference type="OrthoDB" id="10316847at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="146" mass="15239" checksum="14772864A61AB8C9" modified="2019-11-13" version="1">MRYTTTPLTRKKSFWSVNLFEQKESLLFDEVEVSIGDKPAGNKPAEAKPASGKPRDKPADAADGKPVGDKAVTEKPADVKPAEGNPADKRPAGDKPADVKPAEGNPLASEKPEPSNKKPAGDKPADANTVGEKPEDDGAGNPTGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S2D6</accession>
    <name>A0A5B0S2D6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclin-dependent kinase 8</fullName>
            <ecNumber evidence="3">2.7.11.22</ecNumber>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SSN3_1</name>
        <name evidence="15" type="synonym">SSN3_2</name>
        <name evidence="15" type="ORF">PGT21_011164</name>
        <name evidence="16" type="ORF">PGTUg99_023847</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="16 18">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="3"/>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S2D6"/>
    <dbReference type="OrthoDB" id="6284126at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF495">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="20"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="381"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="464"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1080547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1131515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="507" mass="57572" checksum="3DE23870FA5E19D9" modified="2019-11-13" version="1">MMKAYRDRRDGQRQSIQDKYSILGFISSGTYGKVYKAQPRNNNTNNTVVAIKKFKPDREGEVSYTGISQSACREIMLNREIQHENLTALQEVMLQDKSIYLVFEYCEHDFLQIIHHHSQSRSNIPEATLKSLLYQLLNGLHYLHANWIVHRDLKPANILVTDRGIVKIGDLGLARSYHSPIQSLYNSDKVVVTIWYRSPELLLGGRHYTPAIDLWSVGCIMAELICLRPIFKGDEAKPDPHHHHHHHHQHHHSSNKKHNQNLLAHSNVPFQKDQLSKIFDLLGTPTKEEWPSIVHLPEYPNMSRFEKTTNGLRQWYMTKTTSHRTTPGPPTTPTPTTTTTAAAAAAAAELGYQLMSGLLRYDPAERTSAERALRHEYFSRAEPAASRASFLPPPASSSSPSDPHQPKLQTHHHHLHPIKYPLRRVSPDDTDPIMANDHSNFIHNPQRNLLLRPPFQHPHHLHNNNKPNSRPPSSSSLADASALPPPASHYSSSRPASSSCSKRARYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S346</accession>
    <name>A0A5B0S346_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Threonine aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GLY1_1</name>
        <name evidence="7" type="synonym">GLY1_3</name>
        <name evidence="7" type="ORF">PGT21_026264</name>
        <name evidence="8" type="ORF">PGTUg99_009678</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S346"/>
    <dbReference type="OrthoDB" id="10261951at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06502">
        <property type="entry name" value="TA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000030">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041359">
        <property type="entry name" value="GntG_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="L-THREONINE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="6">
        <location>
            <begin position="76"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="26"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1080956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1131114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="435" mass="48248" checksum="C374ADFEE59A0860" modified="2019-11-13" version="1">MAIDCEAGTQTASEMANDLSSHLTIQNTNGQMNHGQANGKKNPLKTNNSYVQLNLPTGGYREPESNEEKRRLISRDFRSDTMTAPTEEMFEFMKNASIGDDVYGEDAMTNRFQEKIARLTGKEAAAFCVSGTMTNQLAIRTWLTQPPHSVLCDSRAHIHNYEAGGIAFHSQATTIPVKPKNEHTLTLEDVQESIILGDDIHYAPTKLVCLENTIRGLVHPLEEMRRIRDFCHENEVITHCDGARIWEASAKTGHSIAELCEPFDSVSLCLSKGLGAPIGSVLVGPHEFIKKVRWFRKMFGGGIRQCGSIVAAADFCVTYNFPRLTETHRLARYLADQLVALGVRIAFPVETNHVFIDPSPIGIEIEELSRRALEELQIRISGARLIVHIQVSRQAVEELVGLVRRMKSSSAPSDSVSEEDEPGLSILTSPTTHRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S4X2</accession>
    <name>A0A5B0S4X2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_028374</name>
        <name evidence="5" type="ORF">PGTUg99_020551</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1133000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S4X2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138249" evidence="3">
        <location>
            <begin position="20"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1084456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1133000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="164" mass="17907" checksum="8E9F275A82C647E6" modified="2019-11-13" version="1">MLLLQWLIITCGLMTPAFSLPMVGESAGKSAAQDVPAITAANTHTEEPVVGTSAKKFTSSDSPDPQNSLRSQPTNADEIEKNSLAQACHPNETTDQASKAKLEKQDRFFEVFMAGTIPAFISLLFMLGNPEPSDRLCTCIWCFFTFLWVYLSSLGSIIYSTGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S7S2</accession>
    <name>A0A5B0S7S2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007601</name>
        <name evidence="3" type="ORF">PGTUg99_021315</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S7S2"/>
    <dbReference type="OrthoDB" id="366230at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="98" mass="10794" checksum="29892D4384B69127" modified="2019-11-13" version="1">MNICPWPVVPATLNRLANRKSLDLNLDTGPQPMLAPANQPLHQPQLKQPNRQPLRLICISQKGKTIRCDERTTHPRSPYGNPPTSDPSAPHPLPPGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SC04</accession>
    <name>A0A5B0SC04_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Mitochondrial carrier protein ymc2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">YMC2_1</name>
        <name evidence="11" type="synonym">YMC2_2</name>
        <name evidence="11" type="ORF">PGT21_026770</name>
        <name evidence="12" type="ORF">PGTUg99_015025</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000042">
        <property type="protein sequence ID" value="KAA1135045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SC04"/>
    <dbReference type="OrthoDB" id="409586at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF12">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="20"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="117"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="219"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1093152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1135045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="314" mass="34044" checksum="3228EFC721C05917" modified="2019-11-13" version="1">MGAESVPTDSSRPTPASSSNQVYKDLFAGTIGGITQVLAGQPFDIIKVRLQSSNQYKSMSDAAIQISRNEGFSGFYKGTSLPLIGIGACVSIQFGCLEASKRAFKERNLANGNGPQLSLGQLYLSGAAAGVGNSIISGPVEHIRIRLQTSSSPAQLNHFVGPGDALKKIIRSDGILRGLYCGQLITVAREFHGYGMYFLGYEWLVERHMKLKDCSRSEIGTGWAMLYGAGAGYSMWLSSYPLDQIKTRIQTDGLPSQSGSRKYFGILDCVRKIYVNEGYRGFLRGLTPTLIRSPLVNGATFAAFETTMRFLNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SFS7</accession>
    <name>A0A5B0SFS7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SRB8_1</name>
        <name evidence="10" type="ORF">PGT21_021142</name>
        <name evidence="11" type="ORF">PGTUg99_002874</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000021">
        <property type="protein sequence ID" value="KAA1136831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20828at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019035">
        <property type="entry name" value="Mediator_Med12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09497">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01281">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med12" evidence="9">
        <location>
            <begin position="131"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1123"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1656"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1770"/>
            <end position="1856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1135"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1675"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1703"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1770"/>
            <end position="1786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1066095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1136831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1856" mass="209062" checksum="3506C58267050CD3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LGQ9</accession>
    <name>A0A5B0LGQ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006302</name>
        <name evidence="2" type="ORF">PGTUg99_005261</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000517">
        <property type="protein sequence ID" value="KAA1064007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LGQ9"/>
    <dbReference type="OrthoDB" id="10417619at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036136957" evidence="1">
        <location>
            <begin position="27"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1064520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="140" mass="15691" checksum="9FD6F16A173F2C0E" modified="2019-11-13" version="1">MHLDGFTARSLVAMGMLLMMMKRVSAPSPLSADLCRGPALARGSPAVEQVVHKPNCQSTGAMSEYQLWIRRDPCQLCPNEVRDRHMAVICKRCLGWTPVHNPLCDNCYKIIDSFRAIICDYSQYGDGFDQLTQALTLHHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LIH9</accession>
    <name>A0A5B0LIH9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018526</name>
        <name evidence="2" type="ORF">PGTUg99_013993</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000516">
        <property type="protein sequence ID" value="KAA1064085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LIH9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="145" mass="16574" checksum="317D2739B0E1F130" modified="2019-11-13" version="1">MNRRADPCHLTERPHSRPQRDRASLPVTRFVFFKRSNPSLQTNQCYQLVMLINDHDGNHHQPASRLLSRYQIWHQDSSSTSRVSQRNLVILASTLSPIPPISNTAHISPIRPNTPKPTQHLHHQSNHLPLYNHQDGPSDTSKCAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LL06</accession>
    <name>A0A5B0LL06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LL06"/>
    <dbReference type="OrthoDB" id="10293291at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022865799" evidence="2">
        <location>
            <begin position="27"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1064776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="179" mass="19208" checksum="AD3C3C9548962BC0" modified="2019-11-13" version="1">MAPLPYIFKMVLIPLYLFAFFMKVGSQPIFPQKIASHLMPRHPDTKNPSPDSQGHSLSDRAVLPIEPDSLRRRSYGEGMVPSTQVSSLSSHTGSGVAGPEQSITTPSNSLPGISRRAALYPKAVATSGWNSRGSPQVGGSLATSDSVRQNIGSGQAVGPHDKPTRTISVCMWDPSYSCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNG7</accession>
    <name>A0A5B0LNG7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_014140</name>
        <name evidence="3" type="ORF">PGTUg99_013045</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNG7"/>
    <dbReference type="OrthoDB" id="1708389at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037847">
        <property type="entry name" value="GRAMDC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37402">
        <property type="entry name" value="GRAM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37402:SF1">
        <property type="entry name" value="GRAM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="648"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="648"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="665"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="791" mass="88129" checksum="63A19D03C8A80A5B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXS9</accession>
    <name>A0A5B0LXS9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_005323</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXS9"/>
    <dbReference type="OrthoDB" id="2533084at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001494">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF22">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="444"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="507"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1068906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="61779" checksum="84F50F38DDBFA2E1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZP8</accession>
    <name>A0A5B0LZP8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_027281</name>
        <name evidence="6" type="ORF">PGTUg99_017827</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000105">
        <property type="protein sequence ID" value="KAA1130968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZP8"/>
    <dbReference type="OrthoDB" id="8026949at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="425"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1069529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1130968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="475" mass="52425" checksum="70011FA723F8D729" modified="2019-11-13" version="1">MATSSTPASRSQPNTPPPGTPSASRPPSRHSTRITTPVRNDPNFVRPSNDSRKALPPSSATQTSARQTRKRPQRPLSASASTLTRAARKTKKAKASQTHKKHKSKRVFVSSGDSESESDSESSSQSEPETHGTAKMIDFTQDSDDANRRFKLHKTKAQPKESEFDKIEEYFHPPVFEEGNFLIVKSHNCRLVDHVKSYSSQLVINRIVPSGISDYSKNVRLPSSTECILLDLHCRIVGWNCIGLPRWRSRLEDVLGMQNTLDIVKGTLPCPKEDSKDVNTSSSRPVDSKGYNPKEIKEDWDALSEMACSTIRLTLTDPLAQQYRKVKPASRLFSTIVNAYEKNTRALAADELLSIGELPTDRQITDRLVGGLDSSWSAVWDSIMYTAIKMSLDDTIGALEAHEVSLNGNTQHDLVSAASAKHLACSNCGKRGHRLSDCRKKNHVKTKASAATTVKLGGYDSGSFDDDDEIGVIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1A6</accession>
    <name>A0A5B0M1A6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017928</name>
        <name evidence="3" type="ORF">PGTUg99_012296</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1A6"/>
    <dbReference type="OrthoDB" id="10460941at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="317" mass="36262" checksum="008B53FFEDBC9B0D" modified="2019-11-13" version="1">MSAQKANSNDSSYHSWTTTTYSLPLKFLLLSSLTSLMLGLVLGDVGQIANVANSASRAVADSQHEEQALSVAERGRSDVSLINWRPGKDEVILPGDANKFQGHPSPATERTPKTKSYWGRFKKAFKKLMDWIFNVLRIRSKKSGNTAQTSAEKVENVAKAEIPKDHQTPMKEVGPVQIREHHPAPVERTDLQVIQDKESQANDEQNMYREHPHQDQEKYTEIPKHHLALLDDKDTQFFRTKNFIVNDEHNVYRALAHLQYQDQEKYALVKDEIEEYIRTNPLKFTKYQMTESDNKEVYPHKISVGGIEYGKMNEHGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M918</accession>
    <name>A0A5B0M918_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032793</name>
        <name evidence="4" type="ORF">PGTUg99_019360</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M918"/>
    <dbReference type="OrthoDB" id="10278644at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366116" evidence="2">
        <location>
            <begin position="22"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1086365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="221" mass="23254" checksum="F6A05BF1F1AAC59F" modified="2019-11-13" version="1">MNTFTVFAALLLLSLEISAHSHDSYTSQPRPNLPASESYNSPSLPSHAVVPTHNLTPLKGSNSQVSNGSNGYLEPVFSGHATPQPDSALHGGANSGAYAKSGATTGRPVHPNTSLPEEGTMVPGAVDTKYGSALNSGGEPKKAGAIPDYIPTFQPFGGRQSAPVSPAYVAPSPKRNVIAARTSRCFIPSRCRAPVHCVSPASCRTLAKYCWKKARRCGYTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9N2</accession>
    <name>A0A5B0M9N2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rnh202 triple barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026581</name>
        <name evidence="4" type="ORF">PGTUg99_024044</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9N2"/>
    <dbReference type="OrthoDB" id="29098at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.530:FF:000008">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040456">
        <property type="entry name" value="RNase_H2_suB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041195">
        <property type="entry name" value="Rnh202_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383:SF11">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17745">
        <property type="entry name" value="Ydr279_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rnh202 triple barrel" evidence="2">
        <location>
            <begin position="8"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1066253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1072730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="335" mass="38103" checksum="751F977B2E8A5524" modified="2019-11-13" version="1">MAASDPIILPTCLLEVKDDLPIVRLPHPRTRLPALFAIKAGQGIWEIQSIVSSPTSSRSWFFRPRREDQIDQLISQQGGRLLVVTPFDPFFLLLGLFLGHANSGNETDPFAIDKKSTDHRARFEDIETILERIQEHWLFAGSAEGPTAGDVELFTNEAFQDKHLSRIFAPLHQEETGTTLWRLEAERIVKEIRRKVDQLHSVGLDAGRDARTVWTRLGSKEGLFDFEQTSQNLPIINDIYKKISLEMIQAYLPKPISEHLQGMNEYQFLALRKEKENQARRSTINPLEIGRNPQGSEAKSKSTAKPPPAKRKKTEDSLKPKPKCTLHQFFKPKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAF0</accession>
    <name>A0A5B0MAF0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007824</name>
        <name evidence="2" type="ORF">PGTUg99_026406</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAF0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="71" mass="7738" checksum="2D0E135301EBB938" modified="2019-11-13" version="1">MNTARIEHQRQKKKTIAKVRALGSLDPQRLTLEGLSFKSSSKSPGCAPESEYDQSTPSTAPTIASVLYRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBS7</accession>
    <name>A0A5B0MBS7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBS7"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF148">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C X DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="2">
        <location>
            <begin position="271"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="634" mass="70523" checksum="238ADE553D98EDF5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCH2</accession>
    <name>A0A5B0MCH2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_017530</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCH2"/>
    <dbReference type="OrthoDB" id="2506775at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1073604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41684" checksum="367B66B3653CEC18" modified="2019-11-13" version="1">MPKITEENEDGNEGDNEDSNSNSEAGSDHTEEDDTESDDDDEDDTDESQEANKGAKDGQKESSSKANRNNSNDLNDLVTALDFVVKKITGSCAWRQEFERQASGKGLKNLIAGYGIRWNIIYESRTRAYEAREVIDAILHDEYDKYKQQRSKKSRKENPKKLGHFKEIQFTKKEWAMINELNEELEPFNRLTKLMEGDGPTGAFILPNYYKIISELKNKEDACNRGHPMHPMYVKMIKKLETYENEALECHTLIMATLLHPKLRLKAFTHCWPEKADFARKLQEKEFAKRVEDLKKQKEDNIQLVKKEAPPVEQSDIFEMFNAPARTDESKELEVYINNMDRLPLPASNDPKSILIWWKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCV6</accession>
    <name>A0A5B0MCV6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Golgi to ER traffic-protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024842</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCV6"/>
    <dbReference type="OrthoDB" id="10271212at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022963495" description="Golgi to ER traffic-protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="58719" checksum="5BA11228D0A091F9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEZ0</accession>
    <name>A0A5B0MEZ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEZ0"/>
    <dbReference type="OrthoDB" id="2496723at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="809" mass="93298" checksum="1EA719BA717E9BC6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJA9</accession>
    <name>A0A5B0MJA9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034989</name>
        <name evidence="1" type="ORF">PGTUg99_036308</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJA9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="129" mass="15102" checksum="098C22DE73FEBC06" modified="2019-11-13" version="1">MQLASVLSAFHMLHYYVISAHPILYPKILEEREVEGASVDNQLKSLYKRMEGSSQGGRMTDDEIVAQMKEATRPYMKMLDFVARKEEELKIRSTESDKNLKHQYDNFDPRVVDEDSKDIKHLDLHFVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML19</accession>
    <name>A0A5B0ML19_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML19"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="11270" checksum="623EE97BB17F45C1" modified="2019-11-13" version="1">MPPRTRLKTHATGDQDRPSDTPPPDQPHDNNNKEVGRTLSKEPTSPHHSNHLLTNAEELERACKVATNTVSSSYNSYKVPELSDQKDKSGRFMIAYHCKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMI2</accession>
    <name>A0A5B0MMI2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATG22_4</name>
        <name evidence="12" type="synonym">ATG22_3</name>
        <name evidence="12" type="ORF">PGT21_002165</name>
        <name evidence="11" type="ORF">PGTUg99_005952</name>
    </gene>
    <organism evidence="11 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="11 14">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Vacuolar effluxer which mediate the efflux of amino acids resulting from autophagic degradation. The release of autophagic amino acids allows the maintenance of protein synthesis and viability during nitrogen starvation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATG22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000452">
        <property type="protein sequence ID" value="KAA1077199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMI2"/>
    <dbReference type="OrthoDB" id="192733at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032974">
        <property type="term" value="P:amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17483">
        <property type="entry name" value="MFS_Atg22_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044738">
        <property type="entry name" value="Atg22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024671">
        <property type="entry name" value="Atg22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050495">
        <property type="entry name" value="ATG22/LtaA_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11700">
        <property type="entry name" value="ATG22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="522"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1077199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1080319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="578" mass="63169" checksum="BCAB0264E5286486" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP06</accession>
    <name>A0A5B0MP06_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MP06"/>
    <dbReference type="OrthoDB" id="447103at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="21"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="635"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="645"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="840"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="858"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="873" mass="93296" checksum="59D8EAEB63D6A94E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQM7</accession>
    <name>A0A5B0MQM7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SGS1_115</name>
        <name evidence="13" type="synonym">SGS1_96</name>
        <name evidence="13" type="ORF">PGT21_026750</name>
        <name evidence="12" type="ORF">PGT21_030876</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQM7"/>
    <dbReference type="OrthoDB" id="2507066at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0347">Helicase</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="122"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="349"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="789"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="710"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="789"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1078219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1114898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="819" mass="91296" checksum="9056689EF5768225" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVA1</accession>
    <name>A0A5B0MVA1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026177</name>
        <name evidence="2" type="ORF">PGTUg99_008914</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVA1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1131109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="88" mass="9932" checksum="A715AADE752D0442" modified="2019-11-13" version="1">MPVGHCPSEKQGGRSSCEAVRQIRATNNTLTWLNMMIPIRKRHGPQPTFVARSLASTTQKVYCMAFVRIVFLPQALAEEFRGINYPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVX5</accession>
    <name>A0A5B0MVX5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_004987</name>
        <name evidence="6" type="ORF">PGTUg99_022856</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVX5"/>
    <dbReference type="OrthoDB" id="2533084at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001494">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF22">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="444"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="507"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1080208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1103937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="61810" checksum="EF06F87F1AD61ADF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWB6</accession>
    <name>A0A5B0MWB6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWB6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="7719" checksum="3173DD962DB2856B" modified="2019-11-13" version="1">MSAIDSARPEAVPTGSPMRPGQPHPARLVFSRHRADLPRNPSTGRILPPSMATVRTENARQSTAGTSEVSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYK3</accession>
    <name>A0A5B0MYK3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_031261</name>
        <name evidence="17" type="ORF">PGTUg99_032846</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="17 19">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYK3"/>
    <dbReference type="OrthoDB" id="3229693at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010435">
        <property type="entry name" value="C5a/SBT2-like_Fn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF66">
        <property type="entry name" value="SERIN ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06280">
        <property type="entry name" value="fn3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 9 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366200" description="Peptidase S8/S53 domain-containing protein" evidence="12">
        <location>
            <begin position="24"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="13">
        <location>
            <begin position="148"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="14">
        <location>
            <begin position="404"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C5a peptidase/Subtilisin-like protease SBT2-like Fn3-like" evidence="15">
        <location>
            <begin position="635"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF06280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1081204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1131366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="931" mass="100520" checksum="5FC8478CD2C08201" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N121</accession>
    <name>A0A5B0N121_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dipeptidyl-peptidase V</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_020998</name>
        <name evidence="8" type="ORF">PGTUg99_028780</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N121"/>
    <dbReference type="OrthoDB" id="416344at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000028">
        <property type="entry name" value="S9 family peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF13">
        <property type="entry name" value="DIPEPTIDYL-PEPTIDASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="6">
        <location>
            <begin position="538"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1082941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1124126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="755" mass="84297" checksum="F2AC7A578D24E0B0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3J3</accession>
    <name>A0A5B0N3J3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005771</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3J3"/>
    <dbReference type="OrthoDB" id="2506106at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099:SF7">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="143" mass="15650" checksum="A830A44D1BF6D0BC" modified="2019-11-13" version="1">MTTKTDYQANLKAELIKGFAAITTPGSFAAWEALPTTPPAGLSVDGVGQIDMPLSEGQIRELIAKAHQAPYGHRSETLVDLSVRNTWEINGNQLSFLDPAWQGYLLKLSKTVASKLGIMGPIRAELYKMLIYEKGAMFKAHTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3U6</accession>
    <name>A0A5B0N3U6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010896</name>
        <name evidence="1" type="ORF">PGTUg99_022528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1066980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3U6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="149" mass="16634" checksum="590804B0E6E11B55" modified="2019-11-13" version="1">MQGIAYRDSLRVGLQFGEQLSSTCRGGFFTGKRVEYIHHGASFLKLAPSAGRFPEENVQRMRSERPRDALASQHHLDASKSIQVTLAKYEASDGRYDLSSIGHSPTKSVRWMTVFSVSIGHFELQFLIGAGGRWSKKASKVRSMVEKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5X8</accession>
    <name>A0A5B0N5X8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5X8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="143" mass="15300" checksum="630142C9BCC89312" modified="2019-11-13" version="1">MSKLAAAIPTPSTQPAEPSPVQSTCPPTAPSAVRSRVVAWPSGPRRVLVLSYPVTGGLAIPRLSSSLKLYRAVGYTAFGCMGTAPYCRTHGKTRSTNSDRQSDSSLKHKSPRRVLNESSYDPRQSETNQAETKPASSVTSCQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6Q4</accession>
    <name>A0A5B0N6Q4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_027685</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N6Q4"/>
    <dbReference type="OrthoDB" id="10020333at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14019">
        <property type="entry name" value="STKc_Cdc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002759">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF23">
        <property type="entry name" value="CDC7 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="238"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="277"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="366"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="630"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="137"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="379"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="647"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1084432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="868" mass="99534" checksum="4D015A8DABA36F17" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBF4</accession>
    <name>A0A5B0NBF4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">E2 ubiquitin-conjugating enzyme 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ubiquitin-protein ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">UBE2G2_2</name>
        <name evidence="15" type="synonym">UBE2G2_1</name>
        <name evidence="15" type="ORF">PGT21_020953</name>
        <name evidence="17" type="ORF">PGT21_021701</name>
        <name evidence="18" type="ORF">PGTUg99_003150</name>
        <name evidence="16" type="ORF">PGTUg99_016809</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="16 20">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000480">
        <property type="protein sequence ID" value="KAA1071115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000386">
        <property type="protein sequence ID" value="KAA1090372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBF4"/>
    <dbReference type="OMA" id="APDGMFT"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23796">
        <property type="entry name" value="UBCc_UBE2G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000008">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0882">Thioester bond</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="14">
        <location>
            <begin position="10"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1064988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1071115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1085854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1090372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="178" mass="20193" checksum="885C98BBD1BD4C0D" modified="2019-11-13" version="1">MHNPRAQATTALKRLMTEYRQLNNKNAKKSDNEEAMFVAGPISESDFFIWEALVAGPQDTPFEGGLFTAILTFPRDYPLSPPKMTFKPPLLHPNVYTTGDVCISILHPPGEDPNQYESASERWSPVQSVEKILLSVISMLAEPNVESGANIECCKLYRDDRKMYEKTVQQFVRQQLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NCT1</accession>
    <name>A0A5B0NCT1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NCT1"/>
    <dbReference type="OrthoDB" id="2510313at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="11043" checksum="4DCBE88FD2E187F1" modified="2019-11-13" version="1">MYSAPTDPRRRRSLAGDDHPAHTHTANTLSLSRKTHSGRPLIIPHCPTMPNQSTALSSRKKQKTRYHRQSPTKTPVPAATAARTATILSPDFLPAAPATTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NES2</accession>
    <name>A0A5B0NES2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NES2"/>
    <dbReference type="OrthoDB" id="10390140at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="443" mass="48063" checksum="97D9FA39849AF376" modified="2019-11-13" version="1">MSFLEFVISSLNGPHYHPLVSSRPFLIDRSSLLLVLANLARYLSAFYALYQRLHELDLRPKSPSNLRGVNGVPLGKLDFAAASSHSSLQYIKGLSNCPSQLLPQFDFQQRHPQASSCPPQVNDGASVIRSGFQAIRSASTSSPTEIHANRFMISGPVRPFTPVNSPETKVPVPIHPSTMAPGSSRAQEHVDPHLGINFYPIQGRWSPVLTSSAGNFPPGLRSPDFVNPSSTDGSSAADSESSSSSIHDGFSSGTESNSSAPNSGPPSIPHSSDFPSPSSSDMRSVPPSEVFYATRPRPQPVATGSGNSYDDPMVVDDDSASGSNPDSVPFGPTTEWGNTQPAAPQINTANPDPSAREMMAFDYRAIRDQLVGSNPLPFGTQVYREEDLLFLFQHIANNFIHLSNKYPASTLGEIEDRVQFYQNLQWVFTADRQVFLATHCGYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIA4</accession>
    <name>A0A5B0NIA4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008370</name>
        <name evidence="1" type="ORF">PGTUg99_030220</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIA4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1088473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="137" mass="14611" checksum="B83E49F6DF54E7AE" modified="2019-11-13" version="1">MSSWDEYPLVGRGFSRREGVYTSSSGGIPPGELDCVPARREGFLPTRWDEYPLVGRGFSRREADNSGQYSSTASGGVASLGTGYPLGYPDIRQVWAEKQPSAAETAPAGGYPLALAGIRQRIADIRGGLSATISKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJK4</accession>
    <name>A0A5B0NJK4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">26S proteasome regulatory subunit 6A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RPT5_1</name>
        <name evidence="14" type="synonym">RPT5_2</name>
        <name evidence="15" type="ORF">PGT21_006773</name>
        <name evidence="14" type="ORF">PGT21_007176</name>
        <name evidence="13" type="ORF">PGTUg99_025326</name>
        <name evidence="16" type="ORF">PGTUg99_031908</name>
    </gene>
    <organism evidence="13 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="13 18">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000405">
        <property type="protein sequence ID" value="KAA1088742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJK4"/>
    <dbReference type="OMA" id="NKISHEH"/>
    <dbReference type="OrthoDB" id="9443236at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000009">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000076">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000037">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 13" id="KW-0647">Proteasome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="240"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1088742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1097445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1101094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1134193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="460" mass="50923" checksum="D70A60B907E647BF" modified="2019-11-13" version="1">MSDQPPPPQENQPSSSKPSADSNNPQPSSSSNSKESTSEDVKMDAEPELDPEILKASPEEIMTRTRLLENEIKIMRSENLTLSHEATMMLDKIKDNTDKIKNNKQLPFLVANVVEILDIDPDVHESEEGAQVDLDSVRKGKCAVIKTSTRQTIFLPLIGLVDADKLRPGDLIGVNKDSYLVLDTLPAEYDSRVKAMEVDERPTETYTDIGGLDKQIEELVEAVVLPMQQADKFKTLGIKPPKGVLMYGPPGTGKTLLARACAAQTKACYMKLAGPSLVQMFIGDGAKLVRDAFELAKEKAPAIIFIDELDAIGTKRFDSEKSGDREVQRTMLELLNQLDGFGSDDRIKVIAATNRIDILDPALLRSGRLDRKIEFPLPTEDARARIIEIHSRKMNVGKGVNFEELARSTDEFNAAQLRAVAVEAGMIALREGANEIHHEHFLSGILEVQSKKKNDQFYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMD6</accession>
    <name>A0A5B0NMD6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMD6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="186" mass="21019" checksum="BBC6E6A5348E1FAB" modified="2019-11-13" version="1">MAGRFKLTNPVHINKSFYSAKAGTVGLNRKGTDRFSPDHICSRWLEWNSAGAPSVELWMPSWLEICTCKSSWLNEGERNSGWSVKTNHRSTHNSWLRFFSHQLAQIHRAIANLSQVSGCGGRIWQRSAGQAVPEGNSPQGRLGPEVDKLRDAWVSFEETIKMLQSLEEAVLRRREILGSQRSSLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0Z7</accession>
    <name>A0A5B0P0Z7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036773</name>
        <name evidence="3" type="ORF">PGTUg99_029945</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0Z7"/>
    <dbReference type="OrthoDB" id="2503998at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="266" mass="29973" checksum="F53923555B2694F4" modified="2019-11-13" version="1">MDPAQKEDYERLSTNLIAHTRFQNANQSNGNTLGGKMFSLGWRKGYEHKSKLGVTAIGSKVAKDRSGFLKLFWEIPFINDFLACRFRDVSEKMYCEVKAHHNESKAPSLAPFFFADPNSFCCHLSFTFDNFYNKAHKDRDESPYSFVMWIPANKETGKLIEENLQVEGGEFVFPADGCAIDFSGFNGVVECAWKATEHPHHTLKSSTPSGSPDTRIGISCQLPASAKVALDKIEAGMYDKKEDWTLRDVGKIVMDSLKYDENGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2C9</accession>
    <name>A0A5B0P2C9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034628</name>
        <name evidence="1" type="ORF">PGTUg99_022875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2C9"/>
    <dbReference type="OrthoDB" id="10334260at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="134" mass="14759" checksum="A548A87B062548A7" modified="2019-11-13" version="1">MKNAGTLPDSRLPTNELLTILSYSLFITPFPVLNQRPLLDPTLWKDATLKIAKSCNKMLQTNEPTAQLPSAFAAALAELKADNEAREARAVNRRNEAATQMVTMKRELTSLSRIGEGRAHITQGPSRIDEEQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2F8</accession>
    <name>A0A5B0P2F8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027621</name>
        <name evidence="4" type="ORF">PGTUg99_007343</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1128938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2F8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474185" evidence="2">
        <location>
            <begin position="18"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1128938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="171" mass="18905" checksum="2AFBB1BB502E9896" modified="2019-11-13" version="1">MGLKFLIALAIMGQCSAVGLAGHKNEKDLSGVSRASKIDPGFPGDGCQASTVKKIKKALEELDDNPDPGGNKNLKSPTIISKQENTISVLNTRTKSVSPDINPAIILDPSKEDKRKTEQKFAIEVDGRAYQKLLWEDEKQGYKALAIFSIFFYSMLVSLYYFCYLGSFIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3D6</accession>
    <name>A0A5B0P3D6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017211</name>
        <name evidence="1" type="ORF">PGTUg99_024679</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3D6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1095352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="208" mass="23216" checksum="A743D198CC3A195F" modified="2019-11-13" version="1">MYLWNSAGFCSGTHQIHDLILPLNKWNPERAPCCSGKGHARPPSHLGPSPARWCRLHIQSELDQRAETSADNFDLARCLNPRAHHKRLYGATIATTILVIQESALKSRCGSSRDRENVGSIPATHTRAGIGGSSATAGLWLQHYDWTTSRHICGSPRSDLEASKARFPSEILDFSGLNRHLRGSQIRREVLHSKLQIREDFNIGHPYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P443</accession>
    <name>A0A5B0P443_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P443"/>
    <dbReference type="OrthoDB" id="2498631at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="700" mass="77293" checksum="C2AA43CA73CF0B37" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P854</accession>
    <name>A0A5B0P854_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008017</name>
        <name evidence="3" type="ORF">PGTUg99_013793</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000343">
        <property type="protein sequence ID" value="KAA1099075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P854"/>
    <dbReference type="OrthoDB" id="2496011at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="302" mass="33783" checksum="1D645E47AAF496C7" modified="2019-11-13" version="1">MRAMCVTPSVEKNTPDNYVSLETRFSILDKAVPRFTEEVQNTLQAQIFGISKYYPELNFSVNALPSTAFPPYTNPNNGLAQQEIGNRDILQWSVWLDGFNYTLPENYQDFNHSHLYMQPEPQGKLYISGVEVNPRIFEQGTLDLLNAGKSGLQIAAVGIGGIRPIPPNATLSEVQLLPAPMPLYLNLTESILAIMMDMAGKDLDKRVLGAGYLCTITRTPWKKAIPMLAMIVGSCSGMFGAALTIILFVARRYDSHLISKQEKRETFDSAVSDNPLTESERDELMLESQTDVMKKKPDQILW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8G7</accession>
    <name>A0A5B0P8G7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_019130</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8G7"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010255">
        <property type="term" value="P:glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000115">
        <property type="entry name" value="High-affinity glucose transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF17">
        <property type="entry name" value="HEXOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="103"/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="543"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="629"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="678"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1231"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1247"/>
            <end position="1274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1274" mass="140365" checksum="6D38A868A77B4770" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCD1</accession>
    <name>A0A5B0PCD1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_026307</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCD1"/>
    <dbReference type="OrthoDB" id="2505635at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="6">
        <location>
            <begin position="252"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1097998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="313" mass="35175" checksum="04DB7F495D907272" modified="2019-11-13" version="1">MAHVINLAAHNGLKAVGCKNIEVAGEQEVTLNRMDLNAIVNQPNGANVNLKTIISHIHGLATYVRGSPQRRETFQATINFVNKQNKRRKPIEVQSLRLDVWTQWNSTYLILQRALKLKSVCLIYCGSRGDAPKYALLPVEWEKLKQITQFLKPLHKVTNILCRSKYPTLTMALPIYIALIKTICRIRLQYDAAQLIPAADEMVNKLMKYLILAPEKPAPIYMTPENALAIFKSEAKSFDRSPSRMANDLTAEVNQYIAEVNERESCDTLAYWTQNKKVYPSLSLMASCYLGIPATSAPSEQVSSQSKTIIGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCS2</accession>
    <name>A0A5B0PCS2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035085</name>
        <name evidence="3" type="ORF">PGTUg99_001474</name>
        <name evidence="4" type="ORF">PGTUg99_002730</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000331">
        <property type="protein sequence ID" value="KAA1103587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCS2"/>
    <dbReference type="OrthoDB" id="2507153at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1103587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="525" mass="57172" checksum="FC5B3FAB8E4F3831" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEA8</accession>
    <name>A0A5B0PEA8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">HlyIII-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_032794</name>
        <name evidence="10" type="ORF">PGTUg99_008154</name>
        <name evidence="8" type="ORF">PGTUg99_018589</name>
    </gene>
    <organism evidence="8 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1099020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000072">
        <property type="protein sequence ID" value="KAA1133231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEA8"/>
    <dbReference type="OrthoDB" id="529367at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF52">
        <property type="entry name" value="ADIPONECTIN RECEPTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1099020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1104843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1133231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="33498" checksum="73D08504C3601C34" modified="2019-11-13" version="1">MKRPTKTLKFAELQAWRQDNPYILTGYRPNLPSMVACLKSIFGYFHNETVNIHTHLWGAVLFCLLIFSLSKPSQYIPWGIRSLVDGTSLSSSVTWKDTFVFGTFFLSGMSCLGFSALFHTFSCHSHKVCSTFGRLDYIGIVWLTVGSFYPSIYYGFFCHGKVIATYLIMITTLGAFATYTVVSPAYRSNSGRRDRTIVFIALGLSGIFPVTHGIISHGLLDAANRVGLWWLLGSGATYILGAVFYAERFPERLIPGKFDLMGSSHQIFHTLILMAAGMHYLSVLKAFKFAHDPSNSSCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PET6</accession>
    <name>A0A5B0PET6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PET6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="60" mass="6925" checksum="C19397CF12CBF268" modified="2019-11-13" version="1">MVHGSVSHSQQLCKHSQQLCKHSQQLCKHSQQLCKHSQQLCKHSQQLCKHSQQLCKHSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PH50</accession>
    <name>A0A5B0PH50_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PH50"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023040080" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="613"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="645"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="712"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1195" mass="134279" checksum="154DF8E9DE358209" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHR8</accession>
    <name>A0A5B0PHR8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CHS1_2</name>
        <name evidence="8" type="ORF">PGT21_030164</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHR8"/>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF41">
        <property type="entry name" value="CHITIN SYNTHASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022736440" description="chitin synthase" evidence="7">
        <location>
            <begin position="24"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1100134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="333" mass="36930" checksum="D1E19FF239946162" modified="2019-11-13" version="1">MAAIGAKLFCVCLVTCYSEGAEGIKKTLDSISAADYSDARKLLFVVCDGMITRHGEKISTPDVCVGFLDADPLFGDPMPMSYRAVAAGAKEHNQAMIYAGHYTQVEGRRTPMIVIVKCGTAEEGKIEKKPGNRGKRDSQMILMNFFSRVTYNDRMSPLDYDLFRKIQVLMGVTPDFFEVCLMVDADTTVMKDSIGHLLNCMQHDPLIMGVCGETRITNKRQSWVTAIQVFEYYISHHLAKAFESVFGGVTCLPGCFSMYRLKARRMNDGDWVPILAQPEICREYSQSIVTTLHQKNLLLLGEDRFLTTLMISPGGTPPDEVVHSPDPGLGPMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIR1</accession>
    <name>A0A5B0PIR1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023787</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIR1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="110" mass="12896" checksum="1C9002F0B0F5DAC8" modified="2019-11-13" version="1">MAWLSFLGKCKVLLDTWVKQFTELFCTSFDVFFLCFTALTFPVEQLTATNRSTRSPFAVIYRKFPMFIHHPSCFILQASQRIGCLLDTLSYCFWKRTKAFQTKRARLSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKC0</accession>
    <name>A0A5B0PKC0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035497</name>
        <name evidence="2" type="ORF">PGTUg99_002852</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000070">
        <property type="protein sequence ID" value="KAA1133726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKC0"/>
    <dbReference type="OrthoDB" id="2496062at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1102047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1133726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="130" mass="14738" checksum="B54E469706885B6C" modified="2019-11-13" version="1">MENKALTAGGSQAAAPSPNQVWSCMVAKSPPPPWKQNLKALTWSQFQSGVIERLSPVEDYFDRFLKRQNLAGLIHWIAMISGNSTYSIKNAVEITSPQDFKLFVREALLRFPAKVIFRLEMNDPRETKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQN1</accession>
    <name>A0A5B0PQN1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQN1"/>
    <dbReference type="OrthoDB" id="68020at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044210">
        <property type="entry name" value="Tfc3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="93" mass="10530" checksum="E9FF69CBDF2455D3" modified="2019-11-13" version="1">MLDELLHHLVAEMAMDGEEGCSVARLNGFIESYYARRRARFPAEPTQFVDEAYKNFVWGKLCQLEPVRLGVLEPAPISSEVVEDDSTQEPTQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PS69</accession>
    <name>A0A5B0PS69_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018593</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PS69"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022880205" evidence="2">
        <location>
            <begin position="20"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="156" mass="17658" checksum="BD6723BD99A5AA4D" modified="2019-11-13" version="1">MLKISLQLLLLVLVGKCSTMNEHEFDPSKSLSLSEIFSELLAVGKWSNKMMTGPHELKKLPGSSDNKNVFLDGRNHNQKVSNHKEYKDAIYHDQEISDFERNKVHKEKDEVYQNPEISDFEMNNLPKQEMVRDASVVPVHEPGAAPPGDIRSSTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSL5</accession>
    <name>A0A5B0PSL5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSL5"/>
    <dbReference type="OrthoDB" id="2506685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="467" mass="54023" checksum="8DBB285947D19881" modified="2019-11-13" version="1">MQFLRQYERTADALNATKYNRALQIGRFVRTEELKCQIEAMDGYNECDWDTLKVSMIESWGDDEILSYKEFQIYLQRFSEILDYLVENEDFDSEQDVSLLFISAFPHQLKTNIKQTLINNGQLPQAPDGSQSPPLLKHVAEAAEREIRLEEDYCNYLRTLEAPVHHSPEVTPSISDLAKQLDSLKQQLLEVQLPVECKTPQLFVPMEPTINYTEPTMDYTEPMMDYTEPVIETAATKIKLPDLVERWKAASANVMDTPMELAHTVIEIPEMVLDGLDKENYGDHEEILDRSVQQVKELDQSTQLNNNYVKFLLTENLTEDLEFPQVSKFLWIQNVNAHQPKLFNKQCFKPYLDVYGLFEVFNKIPQHLEHKTIEPEENEETSVILPALGLSHNSTTIKTILETEIKLDSVVEDSIISLSHFISPNISLFRFHLFNELIFQLLLKIPPDRFGQMYTTFKIPRILVGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q064</accession>
    <name>A0A5B0Q064_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035625</name>
        <name evidence="3" type="ORF">PGTUg99_025737</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q064"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366480" evidence="1">
        <location>
            <begin position="24"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="122" mass="13577" checksum="44689E89EBB80BB4" modified="2019-11-13" version="1">MRILATMVSLGLVCTLFTGPALSYECSAPNIEARICPECEHRGQRLDTLGTIMPEPKDYRFTCGTLRGKPYDCDKVISAKVYHCNTCNHWAIFLKGRCTEALHNLSQPYFLPNEEFQGGSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0P6</accession>
    <name>A0A5B0Q0P6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000035">
        <property type="protein sequence ID" value="KAA1106781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0P6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="62" mass="6636" checksum="1B18A13324E3F085" modified="2019-11-13" version="1">MAEASSGSTSLDFASKSQFRPPDHDQVRRGGGHSRNISTSPEAVDSDSDESFQCRGLHSKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4R1</accession>
    <name>A0A5B0Q4R1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutathione hydrolase</fullName>
            <ecNumber evidence="13">2.3.2.2</ecNumber>
            <ecNumber evidence="13">3.4.19.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Gamma-glutamyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Gamma-glutamyltranspeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_003490</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione conjugates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 13">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="13"/>
    <dbReference type="EC" id="3.4.19.13" evidence="13"/>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4R1"/>
    <dbReference type="OrthoDB" id="1081007at2759"/>
    <dbReference type="UniPathway" id="UPA00204"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000001">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.130:FF:000005">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055262">
        <property type="entry name" value="GGT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF9">
        <property type="entry name" value="RE13973P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00462">
        <property type="entry name" value="G_GLU_TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="454"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="472"/>
            <end position="474"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="524"/>
            <end position="525"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="553"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1108191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="651" mass="71852" checksum="D3C8BAFB5EA3A6F6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5A4</accession>
    <name>A0A5B0Q5A4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5A4"/>
    <dbReference type="OrthoDB" id="2499245at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="257" mass="28326" checksum="FFBBBDB81D52005F" modified="2019-11-13" version="1">MDQVTQPRSARAGSHILAPNLATFFAPPKIAYQRLRAALGQAWGIRRLANVKNEWVQQANDDNNGLYTAYYHFCQSYDNLGASLNDPAGDGASRSAMELVQNFCGAMAGLAFICTKNNMKAKQAKLDKLRTVHHVQHVQMLANLLIFGPTAAFTSLHKGPHMTLSNTNALLELGSSVLKQKIALGITTPMPHSTLDNLRIHFLNFLIKMGFNQLDNNGVPQVNWKDVHASFYNDFSPSVIAGIFSQDIEIALAHRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5E5</accession>
    <name>A0A5B0Q5E5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha/beta-hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006818</name>
        <name evidence="3" type="ORF">PGTUg99_035820</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000002">
        <property type="protein sequence ID" value="KAA1138749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5E5"/>
    <dbReference type="OrthoDB" id="4605274at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053228">
        <property type="entry name" value="Stereospecific_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574:SF1">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366476" description="Alpha/beta-hydrolase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1138749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="44770" checksum="CE4DDFA0CCCC75E3" modified="2019-11-13" version="1">MFQNPPSMAKFAALITLSGFISLAFSRDIGVDRSNVLAKRQNQMGIVSGKGQYYDAKATPVGSLPPRTSGDAPWSQGEASYQKSVGCPLGLKNKAKGIVLLVPGTGGDASEAYKSSPYYQGLPSQGFDVCWVNIPNYSLGDMQLAAEFVAYAIKYLAPKSTASGGKINIVSYSQGGPNVQWASTFWPSIRKLVIGHVALAPPMKGTASTILLCPLSNLSGGCQPSVIQQTTGSNYMKAANSLKDKQSAAYALIPTTIIYSTTDEIVTPQTGPSASSQLIGATRISIQQICGILDNPGHFFILGDVGVYGIALDALLKSRPAQASTVDRSYCKKTAQTLGFQIGNLGNDLKFAFRVAIGEERGKMIATQLRTLRVPSEPLLQKYVCDRGYTTSKCASNGFKDKPTNGSVSNLNKTLSDDLMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5Z7</accession>
    <name>A0A5B0Q5Z7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5Z7"/>
    <dbReference type="OrthoDB" id="10290025at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="133" mass="13721" checksum="485A9770FE8BD7C2" modified="2019-11-13" version="1">MANSLPHSRNKTQRDSLTGNRKGLSAAGNRGSPPGPNNQSRSSVQHIGNSGSGRKANKPPPPQACRALDTGSSNSLSGSMHAPKGHAQLGVQRKPSIDKNSGGKSMLNPNAGVFQPGALSAIVSRVSYPQFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6E7</accession>
    <name>A0A5B0Q6E7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DDE-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6E7"/>
    <dbReference type="OrthoDB" id="2505055at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF73">
        <property type="entry name" value="PROTEIN PDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE-1" evidence="2">
        <location>
            <begin position="255"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="649"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="756" mass="85378" checksum="169ED1C44C2D4502" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q882</accession>
    <name>A0A5B0Q882_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FPR2_1</name>
        <name evidence="8" type="ORF">PGT21_033036</name>
        <name evidence="9" type="ORF">PGTUg99_030756</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 5"/>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q882"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044609">
        <property type="entry name" value="FKBP2/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779:SF7">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474392" description="peptidylprolyl isomerase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="54"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1106315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1109367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="153" mass="16712" checksum="5A36A741B5A43A68" modified="2019-11-13" version="1">MNSKLLAKQSFTTALLVLALIHCFTLASEPKKQKQLQIGVKHKPAECPIKTQNGDHLSMHYTGTLDETGAKFDSSRDRDQPFDFTLGKKQVIAGWDQGLLDMCIGEKRKLIIPPHMGYGDAGAGGVIPGGATLVFDVELLDITNRKADPHTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA14</accession>
    <name>A0A5B0QA14_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QA14"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="621"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="708" mass="81886" checksum="D823DE68DF295969" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA43</accession>
    <name>A0A5B0QA43_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">superoxide dismutase</fullName>
            <ecNumber evidence="4">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_005063</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QA43"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000007">
        <property type="entry name" value="Cell surface Cu-only superoxide dismutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022967988" description="superoxide dismutase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="9">
        <location>
            <begin position="70"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1109969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="207" mass="21955" checksum="E2A6BA36DD77069D" modified="2019-11-13" version="1">MFSFGPRFSVQTVLCLSVLGMVQGLSLPSTVGPEGALSDPTGAVSSSTDTPSETMAMASFTGSGITAHFQFSYNPTSSTSRVNVVVTGLKDGFNNPYHIHTYSSAKYGSCTSTGPHFNPTNATAEHCDPANPTLCEVGDLSGKHGNLESRANEFRTTYEDSSLKLSQTMDGILYRSIVIHGPNKTRLACGTIVPSWSSSDDKNVDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAS3</accession>
    <name>A0A5B0QAS3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAS3"/>
    <dbReference type="OrthoDB" id="5413003at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038014">
        <property type="entry name" value="Ies1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287:SF1">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="163" mass="18144" checksum="E9420D33BD22C0C3" modified="2019-11-13" version="1">MAPPKKNRANQPTNQATPSTSKDNPGWALKKLDGEMFTRADIQYDLMANIFNDRTFQFTSPLDQRAISFKELYVETLSRFSKAAKSTVRQLDDNPQWATNFCIASFLINIGRINTTVACKSSITRTSPGSKSNSNLGFFFFCSLSRVAGVHENLPSHPMSPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEC8</accession>
    <name>A0A5B0QEC8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF382 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEC8"/>
    <dbReference type="OrthoDB" id="10260794at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF382" evidence="2">
        <location>
            <begin position="167"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1111525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="306" mass="34385" checksum="1AF6F39A816FD940" modified="2019-11-13" version="1">MAAVALNGNDLNGIHPKIKGLTRGQLKRLKQKQKPKRPTTHSNANGVSSPKNDSADRSDENKEDDESGDVQSLASSVVDDALLGTGELDNSTRELFADVFAKFQPTDQDTISMEEEKPEDPSKGQVIYSDDEMPSDGDSDAPKPDVSKRKQRKMARLTVAELKRLVKKPEVVEWVDVTAQDPKLLVQLKSYRNTVPIPIHWSQKRDYLQGKRGIEKPAFQLPSYIADTGIATQRDAIKEKESEQSLRQKTRERVQPKMGKIDIDYQKLHDAFFKFQVPPVMTKFGEMLSVPTSWGELYTAAFNTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QF58</accession>
    <name>A0A5B0QF58_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Amine oxidase</fullName>
            <ecNumber evidence="13">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGT21_013204</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="12">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="12 13">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="13"/>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QF58"/>
    <dbReference type="OrthoDB" id="5379943at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000006">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049948">
        <property type="entry name" value="Cu_Am_ox_TPQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF86">
        <property type="entry name" value="COPPER AMINE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01164">
        <property type="entry name" value="COPPER_AMINE_OXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11 13" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="16">
        <location>
            <begin position="37"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="17">
        <location>
            <begin position="149"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="15">
        <location>
            <begin position="320"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="11">
        <location>
            <position position="482"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="12">
        <location>
            <position position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1111828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="749" mass="83610" checksum="1487F32A9ED6C4A5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFD7</accession>
    <name>A0A5B0QFD7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFD7"/>
    <dbReference type="OrthoDB" id="2499658at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="46500" checksum="3FC65BD0908BEE20" modified="2019-11-13" version="1">MSFPLNISTAQPNRFVEAPATGVKQNALASCIDGSDGYPTLQSFTSHMETYLSGKKRADRTVITSADLHQIHRLLLDPAALRADPNPSNRAWVKKTFFLEPTPSGTIVCHREKGQHIGRPVAVKELMYFILKEAHASEGHGGRDKTAKAVKNTHSYIRKGLICIFLETCPTCQTRIDAVKKEKNAALAKSLNIRGSMFQSVPQSVDFTESPYFAINKPTIPPELALVPAAPKPYSRSRNASYPSESYSNLRKGQRMLSSGSNLGGYLPIPTRPLLSPSVIMPNTAIASDFSSQDPSDQRKQRASSNYNSATSITQGLAPNAQNPESFLSEPSAWSYQPQTPIFSLGNRSDNSSWEIEFANIWSGNEPNFYNPIPAPLLAPQPQFSASERLKIPFTDQYVSSDNNNLQFNSWSSVSPMTNVFPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJH3</accession>
    <name>A0A5B0QJH3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJH3"/>
    <dbReference type="OrthoDB" id="2509372at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="396" mass="45551" checksum="96B9619999E12CE2" modified="2019-11-13" version="1">MWAKMGHKALPITTIIAVIKASKFLKKLNSYHQAPKINLSERSQLSSFHLEDPQDPIGLLLVSPSVPVNLQENAELIQRYLVILRLRKEETERNNTPTPWEKLSEEEQLLLATYHTHIHQEEDLEQQIQFRSFLREIREKNKQLRRKRYLEKMSTFETGVIRQERASPTPSVDTDNGSVSKESENQTVPNYVAPSQLQRDLEAAKELQARVKSLQMTTLVRKETAPVVKEKSKKNGDLLLSTETETPMDVDKIESESSEVTPTRPSTPEIRVLSKEEQIRLRVKEHVLLWKKCQVAAREGPTPTLRALLTEAQESQKTLQKLIDNKEIESYVKGWNPWDKKKVHFPAPPKKITMNFKKNEPGKRQSSSSINFADPAKWKKATELLQIAAGLYQNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJP0</accession>
    <name>A0A5B0QJP0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027176</name>
        <name evidence="4" type="ORF">PGTUg99_006718</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000079">
        <property type="protein sequence ID" value="KAA1132502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJP0"/>
    <dbReference type="OrthoDB" id="2495341at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137974" description="Secreted protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1132502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="20018" checksum="CCC55332B439398F" modified="2019-11-13" version="1">MFFRPIAITSLFTAVLWFGFGSRVVLGDIENDDEDCQIYTDARSPTATCNGIYKCAGGCTGYVTATNCTRSQQPNDMKPPLTTEKCKLGYGKSSATMAICINEDASFTCYGPATGKAECKGCVDLNHQGPSGPDPHSVSVSGDNSKGGSNTTPIVDTNGPNVLKHSSGATIRINMFIFSLGTLLSVIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJZ6</accession>
    <name>A0A5B0QJZ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adhesin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032043</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJZ6"/>
    <dbReference type="OrthoDB" id="5570013at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="58719" checksum="3D2FB6B116F2F387" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QL40</accession>
    <name>A0A5B0QL40_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029891</name>
        <name evidence="2" type="ORF">PGTUg99_019601</name>
        <name evidence="3" type="ORF">PGTUg99_021039</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000274">
        <property type="protein sequence ID" value="KAA1113931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QL40"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="95" mass="10357" checksum="4BB915CDA0976117" modified="2019-11-13" version="1">MSAAGGGRGGMCGEGLVEFRRAITPQLMAQHYWSDSNNSTSSSQESMSSFVFAFHPLQSAWHMTHPGDLSYGSVYLGPRLLGETWVRNCPQSSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPZ0</accession>
    <name>A0A5B0QPZ0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPZ0"/>
    <dbReference type="OMA" id="CDIRGVF"/>
    <dbReference type="OrthoDB" id="10257049at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08241">
        <property type="entry name" value="QOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF4">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="11"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="356" mass="38179" checksum="18C0EBC4826FEC4D" modified="2019-11-13" version="1">MHAYQIKQNLESLDQLSGCLTQLPDPKPADNQVLIDVKCAGMNFFDTLQVRGLYQDQPPFPFIPGAELSGVVSQNSPIPKGCPFVPGKTRVFGSTQGCYATKACVAWDQLIEIPPGLDFEHSAAIFVTYPTSYAALINRGKLKKGEWCLVHAAAGGVGIAAVQIAKAVGAKVIATASSQEKLAFVRKFGGLSDQDFTLVYDDTPASSPSGAKKKSSMLEWQAQVLKITKGKGVDVVFDPVGLLNKSLKVAAWDCRLVVVGFAGGQIDKIPANLLLLKNAAVTGIFWGAQVKKDPSESVKVWAALLELISRGQLRPVLHTKIYNGLAELPEGLKDLGSRKVLAKAILRIEADPKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ13</accession>
    <name>A0A5B0QQ13_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7918 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ13"/>
    <dbReference type="OrthoDB" id="3237202at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057678">
        <property type="entry name" value="DUF7918"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223:SF5">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25534">
        <property type="entry name" value="DUF7918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="35"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="337" mass="37985" checksum="F024F6466ACE01EA" modified="2019-11-13" version="1">MPINAATGASCTINLLPPPPPTASSNDQIPEKIPCEEYKHETTTNPTTGAVQETVTIESQQACPFEIFIDIKPTAYSTLTANSSSKSAKLKPQDYVIHTILDGISAGYCKRPKSNSDGSVRLSKTYSRDQSSFRSYQFAPITLVDPDDYDPRNHERSRNPTDTICEDEKVIKSLGTIQVDLIRAELVHRRRKPSQNRAAPVPSTNQMKFSERSKKARLLNTAGLSNYSPSNLPPAPMEWHIKKQDPQPFLQFIFKYKPRVILEDEGTIARDPSHAQLTNQDIEDDDHDDEQESDRKPFLKRLKSEATTDNNDDDDENQDRQMKKPKIIDLIEADDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQA5</accession>
    <name>A0A5B0QQA5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Prephenate dehydrogenase (NADP(+))</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TYR1_5</name>
        <name evidence="1" type="ORF">PGT21_036256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQA5"/>
    <dbReference type="OrthoDB" id="5399569at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="150" mass="16861" checksum="01033DEAC2FF446B" modified="2019-11-13" version="1">MNPIAKVQIDQFARSVSELFVLMTSNDDEGLSRRVYAGRDHVFGPPGSPRSPVPPMFLSEESLLEFRNRRARRGSLAPQQPSEILTLLEIVGCCHQLALAIAATLVFRLWFGLIHHLFRSPERLDKAIHAAVHNLDYRTDNSEFVLAARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQY1</accession>
    <name>A0A5B0QQY1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQY1"/>
    <dbReference type="OrthoDB" id="3360715at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="427" mass="46478" checksum="A65CDD82C8A37F59" modified="2019-11-13" version="1">MSSSNLIHTNISEEQVIVAFHSFLQGALAQAHAERLLDTAVLSSAEADIQIAGPAICLFLAALRSEGNPPSIATSMISLDATNCPASFRNWFVLWSESVPKIKALSLELRHDLARIICDQEPQCQPIRIDLPVIAQNLRAIAIEISQRRTFQERFSQDLDYALQFGYDFRAPAPAPTLPSSTFRPPPAYNDNGSNPSTPSSLAPTYAPKTNTSPLSGLDPALEDHPALRIIRETLYASLTDILYTTPTIFEILQSRSSTDGQLSRAYFGALCLAILEVSLTLVSPADRHVRVVHLGSGYPSILKVEDCPPHLQPMAAQLFLIASSTQTLAREDLEAAVENASHGGETAQPKIDRLKTDLEHGTNWEAGVSACDIEDNRPASPAGTVRVAANRINALALGISQIPSFREREEALFEILVPSLSERKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSR1</accession>
    <name>A0A5B0QSR1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disintegrin and metalloproteinase domain-containing protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_003240</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable zinc protease.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSR1"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000003">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051489">
        <property type="entry name" value="ADAM_Metalloproteinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702">
        <property type="entry name" value="ADAM10/ADAM17 METALLOPEPTIDASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702:SF2">
        <property type="entry name" value="KUZBANIAN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13688">
        <property type="entry name" value="Reprolysin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023103414" description="Disintegrin and metalloproteinase domain-containing protein B" evidence="7">
        <location>
            <begin position="26"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="828"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="9">
        <location>
            <begin position="403"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="8">
        <location>
            <begin position="640"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="860"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="879"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="560"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="559"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="563"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="569"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00056552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00074021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1116170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="906" mass="98149" checksum="53853B861140F17B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QU22</accession>
    <name>A0A5B0QU22_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">26S proteasome regulatory subunit 7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RPT1_3</name>
        <name evidence="11" type="ORF">PGT21_022962</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QU22"/>
    <dbReference type="OrthoDB" id="1937997at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000005">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000027">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000290">
        <property type="entry name" value="Probable RPT1-26S proteasome regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048723">
        <property type="entry name" value="OB_PRS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21236">
        <property type="entry name" value="OB_PRS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="249"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1116690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="474" mass="52399" checksum="406997DF07A0972D" modified="2019-11-13" version="1">MPPKQDWEKSKKTSDDIAATDDIIALDEGDIQLLKTYGQGPYARQLRTIEKDLQDLQKRIGEKMGVKESDTGLAPPNLWDTAVDRVRMSQETSCLQVARCTKIIRAPDANATPEDEASRRANGRQPAGAGAGGRTLPGAGMSRPNADEEDKYVINVKQIAKFVVGLGEQMAPTDIEEGMRVGVDRNKYQIQIPLPPKIDPSVTMMQVEEKPDVTYSDVGGCKEQIEKLREVVETPLLNPEKFVALGIDPPKGVMLFGPPGTGKTLCARAVANRTDATFIRVIGSELVQKYVGEGARMVRELFEMARTKKACIIFFDEVDAIGGARHDDGQGGDNEVQRTMLELINQLDGFDPRGNIKVIMATNRPDTLDPALLRPGRLDRRVEFSLPDNEGRANILKIHARSMSVERNIRYELIARLCPNATGAELRSVCTEAGMFAIRARRKVANEKDFLDAVEKVIRQGSKFSSTALYQNYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVZ3</accession>
    <name>A0A5B0QVZ3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVZ3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="124" mass="13994" checksum="A9C0B1A4696ED714" modified="2019-11-13" version="1">MSTTTQQPWQSQASVAYTTVIWLSHRCDATAVVLRTTLSASEKEYIRLARDVRGISVATNSHPSLSLIVLHHQHPRQYVSVVMKKNQSLKINCPIKKNGPDNDLGSFWPLPLRYQPSELDDNPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXL6</accession>
    <name>A0A5B0QXL6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_030589</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXL6"/>
    <dbReference type="OrthoDB" id="1921208at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052953">
        <property type="entry name" value="Ser-rich/MCO-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883:SF15">
        <property type="entry name" value="EXTRACELLULAR SERINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883">
        <property type="entry name" value="SERINE-RICH PROTEIN, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023078215" description="Phytocyanin domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="617"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="535"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="559"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1118057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="640" mass="65458" checksum="D8D7C98CD6DBE85E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYV0</accession>
    <name>A0A5B0QYV0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GPI10_2</name>
        <name evidence="13" type="ORF">PGT21_032401</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third mannose to Man2-GlcN-acyl-PI during GPI precursor assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 22 family. PIGB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYV0"/>
    <dbReference type="OrthoDB" id="416834at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022791235" description="Mannosyltransferase" evidence="12">
        <location>
            <begin position="21"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="465"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="465"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1118143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="599" mass="67577" checksum="1C7A546151D3C9A7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1Q1</accession>
    <name>A0A5B0R1Q1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025624</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1Q1"/>
    <dbReference type="OrthoDB" id="10483706at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="215" mass="23658" checksum="EA4260D129432390" modified="2019-11-13" version="1">MPSPGQLLSMASSPRLLTSTRTPSSPGFDFQRMANRATPPPSLNGVPIPHLGTGDQKSTPTAVPIHVRILRISSSVPLQTRRPGSRDWIGTEDCALTAHLITARCGRTSLRVPIYISESVFSPDRDPNIRTAMEMLLRPSLTVIWCCKRVWEQAPVPRVLAPPRPGPAPRGLDLAGRAGVGPWHWLKSGLAEPKLWAHQITDIDYHHHHTRDPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R256</accession>
    <name>A0A5B0R256_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R256"/>
    <dbReference type="OrthoDB" id="10484601at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="333" mass="36925" checksum="2AEA1D667AB5C5FF" modified="2019-11-13" version="1">MPVRVIKEKSSWDEGRNNEHVKVSTLVQTATRPELRSASFTKIENPETSGGEREAKKHPYFWEENARSVFLPSKTNPSPGSSHSRPAQDGCASGRSPSLLQSPAISGSAQITLSATYNCNLSDPALTLIHNSSVSDESPHEPKLCKRNRKWSDLRGWQQGRQSEITCNTYRAVGYISSKQRPQQGWAQDSEDHCGLLSFRSLSKKHKNHLPELSKEARRVDRSRPTSRTDGWGIFKCWPSMVCMTSEFILPHLSLDTLRGKCADTPPTSICPDMAIPAEKSAPAAPAGSLEPFHGKSEVKPVQPKVKAGNLADPRRGITRTFHEEHQLPTLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2Z2</accession>
    <name>A0A5B0R2Z2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">37S ribosomal protein, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MRP4_2</name>
        <name evidence="3" type="ORF">PGT21_036490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2Z2"/>
    <dbReference type="OrthoDB" id="2320368at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000050">
        <property type="entry name" value="Related to MRP4-mitochondrial ribosomal protein, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_uS2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="373" mass="41056" checksum="E248F15D909547B0" modified="2019-11-13" version="1">MAQLNRYQQLLRQLLSTTTPSKPSSIITRRNSSTTTNTTTTTTTAEITGSLSPSLGSSNNQQNDENIKTIRTQSLIHRMENHARKTIEFRQRLAQDRERLSKLGSIQTTLETSKPSHSRTHPTQISDLTLATLITATTHLGHNKALTCPTNFPLIYGVRSDIAIIDARQTLSYLRRACNVIRETVFNDGIVLFSNGVPGTEKAIKQASERLADNGYSLGIRPNGKGASWVRGTLTNATEVLKRPRQVAKQLRLANPSSSSSSSSNEDPQRNKQQQAENLEALKFLPSLIVLFNPRQSRVLLREAALKQIPTIAIIDSDVDPRVVTYPIPANDDSVRSVQLIAGVLSRAGQEGLLKRKQSLLSTFPGSDSLQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3N9</accession>
    <name>A0A5B0R3N9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_035903</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3N9"/>
    <dbReference type="OrthoDB" id="1663137at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051799">
        <property type="entry name" value="NADH_flavin_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43656">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G08260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43656:SF2">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G08260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="438"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="6">
        <location>
            <begin position="14"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1119893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="466" mass="51407" checksum="C150648804E46631" modified="2019-11-13" version="1">MDSTDQQQIDVIQQPIRLPCGLELTNRLVKAAMAPCLAKAGQPTPEHHRLYHQWSQDRFGLLITENVQVCPRHLATPTDIAIRHGAEKDSIPTSWQAWAKVCGPRCLVQLSHAGLQSPRGCGRTLGDRPIGPSAMRVCLGNRLIDRLVAWALFDHAREASKDDINLIIEQFRAGALFCHRAGFQGVQLHCSHGFLLSEFLSAKTNQRHDEYGMSATNRLRILFQIIDAIRSSVPESFCLSIKLNCSDLSEGGLTQEEALDNLRRIVTRGGIDLIEISGGTYENCPTSKEEVTPQPKSEKKGVDRREAYYVEFTQEARRVLRTISTESRQPCPILMTTGGFRSRSGMAAAISNEETDLIGLGRPACLDPLLSTKILDSNLANYVSPNPPVPGVTLWKILVPVKLVGAGFKTMWHTWQLHRMSRYQPPDLACTALGSLRVILPLDALLASSAILLSLLAAGIVTRLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3U7</accession>
    <name>A0A5B0R3U7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycosyltransferase family 49 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_037225</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3U7"/>
    <dbReference type="OrthoDB" id="411524at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042285">
        <property type="term" value="F:xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051292">
        <property type="entry name" value="Xyl/GlcA_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270:SF25">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1120168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="571" mass="64678" checksum="D290FB099A64CBD1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R479</accession>
    <name>A0A5B0R479_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_037256</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R479"/>
    <dbReference type="OrthoDB" id="2505895at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="183" mass="20677" checksum="BB109387CEB89E8B" modified="2019-11-13" version="1">MQMEISCELDHADFARFEREILGNEVIGARAEINMLRDERTLLLKRLDLNRENITQLMETEEQTYKTLMVERATTEGMIRALKNKIRELKTEQHGAQGQITSLTNNMERAEKARVAAVLYAKGAEDRVVEVANRQKQAASSPPAPPANPTIPFWPAADDEALKNKLKDQMRSVKESDGPVNLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R9A6</accession>
    <name>A0A5B0R9A6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipase B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026127</name>
        <name evidence="3" type="ORF">PGTUg99_036583</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R9A6"/>
    <dbReference type="OrthoDB" id="4605274at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053228">
        <property type="entry name" value="Stereospecific_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574:SF1">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366578" description="Lipase B" evidence="1">
        <location>
            <begin position="21"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="43182" checksum="D9844389C0E81946" modified="2019-11-13" version="1">MGFVAWIFVYLSVFPLNVLSRNVVPLPLVKRQSAQAGIVSDRDHFYDVAAAPTGPLPSRASGDAPWTQAESSYKRMIACPFGIQNKDRGIVLLVPGTAGNASEVYKSSAYYADLPSQGFDVCWVDTPNYSISDMQLSAEFVAYAIKSLAPQSTKTGGKINIVSYSQGGPNVQWASTFWPSIHNLVRGHVSLAPSMKGTASNLLLCPGSNLAGGCLPSILQQSMGSTYMTAANSGKDTRSAAYALIPSTIIYTTSDEIVTPQLGDLASSRLIGASNIALQEICPFSVNVDHFAIPGDVGAYGIALDALLKGRPAQTSTVDRSYCIKTASSLGFQVGNSENNLRFGFRTAVGNQRDQMISSQFRTLRVTAEPPLQQYVCDRGYASSGCTRNGFRGKPTKSVFPLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RC15</accession>
    <name>A0A5B0RC15_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006813</name>
        <name evidence="4" type="ORF">PGTUg99_010108</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000210">
        <property type="protein sequence ID" value="KAA1123217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RC15"/>
    <dbReference type="OrthoDB" id="10620772at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1123217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="111" mass="12063" checksum="A97416923ED80363" modified="2019-11-13" version="1">MLVCSLRKNPPSPSTSAFLLRRSTPAVRPVLPRFLSTESARTTTAKRSAPSPWLFFAIFTAGCSTFVYITHKRSLNDSDPRLRSKALPSFGGAPTVVPSDRPDASSTRPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RGF0</accession>
    <name>A0A5B0RGF0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003897</name>
        <name evidence="2" type="ORF">PGTUg99_019998</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000206">
        <property type="protein sequence ID" value="KAA1123794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RGF0"/>
    <dbReference type="OrthoDB" id="2513962at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1123794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="400" mass="46303" checksum="E5329F60EB2CCA86" modified="2019-11-13" version="1">MAVLKDLKLVEPNLDQSSSNRELSSWLFDQAFKPQNSFPVFGMLETTHHLKPVKEFGRIQKILLEQLSRHDPEEDSLEASILIIQSWYREFHPQIWEHINNNQDGYSPEIYLLLALYKSIGSGSTGECDQEDEKFTDWDSLGSLKIPDLSRFPVTMKPERFLERHLSGDGSIEPSKDLLVFGSTDFVADSIQVEGLSVRIRRYKHRSEDTPSNFKYRIRVGDGSPTDGRLRTIVSKFNLLDTHLSICHREIIHQLKSKGNWVPDDALKSFSQWFKDILLGTEGNSLAIIGDIEKEEDGEFQLDEFGEVQIYLVNQYLGHPMPYSRVIWVSLVLLEYWYRMFHSECFFPTSNDYWNSMIKSLGIKLDHAGRYSLVKYDDQGNFVGRVHRKKSLRSKRLASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJG5</accession>
    <name>A0A5B0RJG5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_016308</name>
        <name evidence="5" type="ORF">PGTUg99_025620</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000173">
        <property type="protein sequence ID" value="KAA1126121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJG5"/>
    <dbReference type="OrthoDB" id="19859at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006829">
        <property type="term" value="P:zinc ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045891">
        <property type="entry name" value="ZIP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39 ZINC TRANSPORTER , MEMBER 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133:SF0">
        <property type="entry name" value="ZINC_IRON REGULATED TRANSPORTER-RELATED PROTEIN 102B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1097656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1126121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="172" mass="18177" checksum="1DCCAB782AAA7308" modified="2019-11-13" version="1">MVHKAPAAFGMVAVLLAEGLSTGAVRRILLAFSLAAPMGALTTWTGLALVDRLTGRPADLPEKAEDAFQWWIGVTLLFSAGTFLFVATHALQNTSSNPVDPCCPQIPANPSPSTHLDPELPSHHLHLPARSPPTPSSSSSQVSVSPLLTCCLMASGMCFPWLLTLLVGSHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RK12</accession>
    <name>A0A5B0RK12_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035397</name>
        <name evidence="2" type="ORF">PGTUg99_027319</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RK12"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1106479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1126331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="127" mass="13959" checksum="178C85DAF2E5368F" modified="2019-11-13" version="1">MNPSPPQLKPNTILILIPVKVCKGCPPSTTIPDQLAHQKPTTTHSQNWIALIEHGDSVFVDKVGYPQNSGAPAVIVCDWQQSPTISSSSLLTLTHILSSILWSTHNLRCRTPGTDRHGKVYVYQKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RMV1</accession>
    <name>A0A5B0RMV1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012991</name>
        <name evidence="4" type="ORF">PGTUg99_018097</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RMV1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138187" description="Endoplasmic reticulum transmembrane protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1126769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15676" checksum="3BD635AB1299B120" modified="2019-11-13" version="1">MLLPIILLAFYFSNYYVTSENPTLHQKTLMNRGIDKGVAIGTQIHLLHRRMEGSSKEKIEEFEKSLGDIGQRIKELEAKANLLDEENRKSDKKFKKKLQKLEQDYNESNRILDEKANKLLREIENHYQGSPSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RVJ5</accession>
    <name>A0A5B0RVJ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022254</name>
        <name evidence="3" type="ORF">PGTUg99_030609</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RVJ5"/>
    <dbReference type="OrthoDB" id="2505396at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="364" mass="40157" checksum="B663FA0052BE7868" modified="2019-11-13" version="1">MGFSFNCPCLCWPTQRNQQQQQHQQEQQQPLYPHSEADADERTPLIVNPPQVTPSPSLPVIDTAQQQADEAAANKIVSQTVRCLVDVYSQAPFSHLKIRGAISASDSISDLPHTPDGAATAWRAYDPPSCPPVSSETSKYQLSASTNFQSPSTNHGHQTAPSKRSFHSLKTIINHQNRSLPDEHDTRLSASLIDLQQDPAHRLSSSSTLPGSTHASTQFASQRHSRDNSFAPTSCSTQFDTIRTFQTARDHPNLNNDDDEDDPDPTEDLTLTTSQFPINNHLNGDLHPPPPLLASKPDFVTRLWDQSDHQEPPHESEESPANLSDQTSCQTDPTDLLTDDPKKLAEMIANGFRLTDTGPILVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RZA6</accession>
    <name>A0A5B0RZA6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005043</name>
        <name evidence="3" type="ORF">PGTUg99_003740</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000116">
        <property type="protein sequence ID" value="KAA1129924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RZA6"/>
    <dbReference type="OrthoDB" id="2507706at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="395" mass="42763" checksum="7686E71396170A16" modified="2019-11-13" version="1">MSTHPAEIADQLASSAKQAPHMPITPPHSSRSAFAELPLTQNFKSAPELSGQTTEEGSDDGMEEEDQDDGEYDDEDECEDREEESFTQPGLGLTPGYDGLGGPVTPEAFTAEDELFGLIEPGHLREAEAALSELTGSISSALTQYRDQFDAWEQLPNPPVSVIRASLLIDQGLARFIFVLEPFKLNATQFHALSGDFNELSSFITQNHLDQHNPTTTTTTNTQLWNGLGRLADEIVQVEDGLSCAMAEISELHDWVDGLLTPALAEVKFSYRATNELVSKILDVHSYLCDSLAKAASILAIYRSVLGHLSRAIDSLQATLLDHLEPSNPLFPFQQTAAWAAAASLSLASFARAIAIFCSISGCIQLHTQGALVSDTPPIGSTAHPPVRFYHAISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1C8</accession>
    <name>A0A5B0S1C8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F-actin-capping protein subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CAP1_3</name>
        <name evidence="3" type="ORF">PGT21_025469</name>
        <name evidence="4" type="ORF">PGTUg99_050139</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1C8"/>
    <dbReference type="OrthoDB" id="541052at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF118">
        <property type="entry name" value="BETA-1,2-XYLOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="2">
        <location>
            <begin position="325"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="614" mass="71002" checksum="C7E86318F589E0C8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1E0</accession>
    <name>A0A5B0S1E0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014988</name>
        <name evidence="2" type="ORF">PGTUg99_032076</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1E0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1131582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="51" mass="5793" checksum="EF5D648B4BF5C7D6" modified="2019-11-13" version="1">MGCDPPVNHRSRILNSNLIQKSTTSLNSIDIHYLRYKRMTSSQVFQSPIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S2C2</accession>
    <name>A0A5B0S2C2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020410</name>
        <name evidence="4" type="ORF">PGTUg99_031974</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S2C2"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="416"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="670"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="786"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="880"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="483"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="673"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="791"/>
            <end position="810"/>
        </location>
    </feature>
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        <location>
            <begin position="838"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="898"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1137" mass="125597" checksum="10828766A042C03F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S676</accession>
    <name>A0A5B0S676_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010652</name>
        <name evidence="2" type="ORF">PGTUg99_003587</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S676"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1133410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="146" mass="15716" checksum="185986590F1F6AA6" modified="2019-11-13" version="1">MIGISWGDPIRAAWELGIGISQADPRSPGLPHSRWVGLGIDRTPPGLPHSSFPAPSLRFIAGYLIWRPPTLVASPFKRRVVLGLDRTPPGLRHSSFPACPPIRRCFAGRAAAAAAVVALGVVGSTWYPPPTTFICFSRCARQLVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SBA8</accession>
    <name>A0A5B0SBA8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028573</name>
        <name evidence="4" type="ORF">PGTUg99_021117</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SBA8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138315" evidence="2">
        <location>
            <begin position="25"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="140" mass="15348" checksum="13EFB3DA2F49ABFF" modified="2019-11-13" version="1">MSNCFPLFLAILKPLVLLPLPSLSLPIYNHHSASLVRSTRDAISQRSTKHPPSPTTQHVDNSLLKKSTTKPTSHTHSLISILLPLPPPPVTRAIQRPCTILLCLLTTPVFSTLYDLSLAQKAKKKEGTTTQTNSPSRLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKE0</accession>
    <name>A0A5B0LKE0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000198">
        <property type="protein sequence ID" value="KAA1064108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKE0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="260" mass="27783" checksum="27BD1B8481E7236D" modified="2019-11-13" version="1">MISSLCIQGAKTVKRSEEWKVPPPKRLARVVNAHQKTVSPSSKPTADEFRSTGRDGAALDPALSADEEDVNVADEEETGSRSSALINKMKSLIKETPKKALKKKTANKSNPLRLGSMDVDPPVTDKHQASTHARGGSMPLTKVGLVLSRPSIIVLTLPRSDVFLVTISFKFDSQGLKSSSAGQKTPKDKMPAERSDKMNIDPPALARLKATFAIPAASTANQLQEPAANPSTPARMENVNKYPAGLTRGGPVTTPVILVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMT1</accession>
    <name>A0A5B0LMT1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025531</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMT1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="61" mass="6667" checksum="7B89206D93FF1255" modified="2019-11-13" version="1">MPIRFRSTLPTSSTNSRLPISSVVFDRIFDALSTGLVGSRGFSSISRIVRVDWLGSAHGWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LND6</accession>
    <name>A0A5B0LND6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019337</name>
        <name evidence="3" type="ORF">PGT21_023902</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LND6"/>
    <dbReference type="OrthoDB" id="2507867at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366014" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11824" checksum="832A75BBD09EE5D5" modified="2019-11-13" version="1">MPIKKALGFLATACLLSAQTVPMPPGAGFEELEQPCTHPTRVWSQFQNSPCPAFYNCHAGHKHRCEYPTNGAGRRCTTCNDYANINYRTPCPLAAQGHPNRPCPGIPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPC4</accession>
    <name>A0A5B0LPC4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SGS1_91</name>
        <name evidence="12" type="synonym">SGS1_88</name>
        <name evidence="11" type="ORF">PGT21_025724</name>
        <name evidence="12" type="ORF">PGTUg99_020424</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPC4"/>
    <dbReference type="OrthoDB" id="2499463at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0347">Helicase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="51"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="283"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="678"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="688"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="716"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1066231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1072706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="753" mass="85405" checksum="B88D02362502A6E9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQT1</accession>
    <name>A0A5B0LQT1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000194">
        <property type="protein sequence ID" value="KAA1066832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQT1"/>
    <dbReference type="OrthoDB" id="2519100at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="346" mass="38372" checksum="D08E3F4A4AA1B166" modified="2019-11-13" version="1">MSNSRAFALDESSAINFKPYDSSNHKAQLVDFIRLVDNHAIIPPKSRLTLCVNLANKYLNILTSLTPSPRGRSNSRPDAASITSKNSIYRCCSITSKNSIYSQAISGKISITGKGLCGSIHQPKPPSRSSKRSAARASVSPSPKQSTTPPPITRKIYSWPITSQTISTIKIYSWPITSQTQTIKAIKTERKPTSPPRRRRSSFPVPRRAHRSPPDTVITLLQIIKIHHNPKISQYLKSPPKRIFRILPNSLGLSVVRLRITDLLHNAGKRRRQFRGDLWLRLHRQEPEPSRVKKHSLAQAGSLSLEASKSTIDVDQSGTDDTSDHGISVSHTFWPSTTTITGLPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LU12</accession>
    <name>A0A5B0LU12_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_001801</name>
        <name evidence="5" type="ORF">PGTUg99_027649</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LU12"/>
    <dbReference type="OrthoDB" id="271725at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="60"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1067343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1137882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="372" mass="41047" checksum="0C11BCA19D87F44A" modified="2019-11-13" version="1">MEQTRWVSAANDQPIPKLDGFQFHLGEQTSPEIPDFNDEHSSSSAIAYLRLPPVHPPSPTNIFLANFPKSWSEYDLAQIFDGIPILTVHVIRDKIASNAGGGLSRGVGFVNVLYKHEAVALVQHLDRKIWLDVEAPLKIRLAKATAPNPKVDVDINGMMDPATRPKEGELSPGRRPGPKVNLLKELEVVVTLAKRPIQSSPSSFSKSPTVPGGLYNLWEPMACNVKPHSNGWPSYTNTKFEPMQFPNEVNHFRSPVHSSPSTPYDPKGILEAYPNQWASWQSNQEPIKPMIFDHCPPMRSYPYSTNGFPKPPSHLPSVPDKSSSPSWPGSNFDYGFIGGPAVDLISGAVISPRRHQRNSSPISSETSIPRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LX45</accession>
    <name>A0A5B0LX45_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">USP7_23</name>
        <name evidence="11" type="ORF">PGT21_024201</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LX45"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000215">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 7, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024729">
        <property type="entry name" value="USP7_ICP0-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12436">
        <property type="entry name" value="USP7_ICP0_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin carboxyl-terminal hydrolase 7 ICP0-binding" evidence="9">
        <location>
            <begin position="46"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin carboxyl-terminal hydrolase C-terminal" evidence="10">
        <location>
            <begin position="291"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="594"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="599"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="616"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1069427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="634" mass="71957" checksum="DCE853BD8B4A408C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXZ8</accession>
    <name>A0A5B0LXZ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024481</name>
        <name evidence="1" type="ORF">PGTUg99_023842</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXZ8"/>
    <dbReference type="OrthoDB" id="2509441at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="622" mass="71483" checksum="8BE32A24175F8914" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LY21</accession>
    <name>A0A5B0LY21_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LY21"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="136" mass="14700" checksum="3D2A438E2BDB152C" modified="2019-11-13" version="1">MPKNPGLIILEHELTIHSVRGFTHAWPAIQKAGWDPRPIPSLFNAPWYQNAKGAYDEAIHLTSLLNASEVVASARARLADTSNSTSSNSPLNSTVPVPPAITSPQVRGSISGTSHKRSAFQILHLIFVQGLILTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZA6</accession>
    <name>A0A5B0LZA6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZA6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022764230" evidence="2">
        <location>
            <begin position="24"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="606" mass="69078" checksum="3A74138E41B63F6E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0R3</accession>
    <name>A0A5B0M0R3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0R3"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="7955" checksum="9D4EEC229FC73B4B" modified="2019-11-13" version="1">MRAIPNQTDMGSDTFPSRQSLRESAGPNQNTEHEMAQPSWPSWSGIRAIANQTDGRSDSTPPDQRSGRPETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3W9</accession>
    <name>A0A5B0M3W9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020703</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3W9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="102" mass="11296" checksum="AC781EB21AA7AC87" modified="2019-11-13" version="1">MNKHGSCSTYSGSSDMNLRQSADTMAIRRRANPYKEQILHLFAGGRYLAMLTECRPHGKKDISPKALGKHRPLPLIAGQRYWASWSSATALMGKNSSQSADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7A6</accession>
    <name>A0A5B0M7A6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024034</name>
        <name evidence="3" type="ORF">PGTUg99_010236</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7A6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1072652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="362" mass="40408" checksum="3880713830A42648" modified="2019-11-13" version="1">MPSLGPGVSNPSWSNQNCGSNTPNALIQAGFADRGSSEVLGASHHQSQQSQANHEENSEDDLARALEASREEYERHQPDIYQSLEEALVRIRSITDIPESNERNSKDVLAHVLEASKEEYEQREADIDQPLEEALVRSLMDESHGNNSEDDLARALKASKEEHEQREPDIDQLLEEALARSLMDESQSSSRESDAFLLDALSDAELEKFQESTRGNHCALGIEDQNLARVIEMSKKEALDRELENSLSQALQLSLKGRNQAGTSHDAASPNKPSYVEFLGSPEPSDTEQKVVSRVFRSRMPAQEHLGQPQNLMDEPLTRHTSPSLVLANKSPGSEFQENYHPNGYSGQPDNPDWRPRYAERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M914</accession>
    <name>A0A5B0M914_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M914"/>
    <dbReference type="OrthoDB" id="2497383at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="306" mass="36186" checksum="234E0742B52C90A7" modified="2019-11-13" version="1">MKNKRRKSADLPKRTLPFKLVSVPFNRNREEDFVLQAIFNLKRVDDTNFIDHYLRGNDGRFKIINIQENQIKEFYNLYHRESRLDYSEDNMSPSGRRRIPEYVLEQRIVRRAKYAKAVENFRGPTEIKELKDYKQHVSKQFSHFIEMIENTVDRTPGEKKEQLLNESNAILGLLPFYLFHVDMINTLIPCEKNKQLVGNGLGAQRESAGKIFLEIARKIIAPGHQPIWTDRNFRREDLSMFARHDFNTATALCSFNWQVLGYWLECTRSSQFHDDVTYANGLIHNQFKHFLNDIIASSMDRYEQSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MD19</accession>
    <name>A0A5B0MD19_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009629</name>
        <name evidence="1" type="ORF">PGTUg99_021633</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MD19"/>
    <dbReference type="OrthoDB" id="10295825at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="57" mass="6505" checksum="53385C2EC946987F" modified="2019-11-13" version="1">MGKQKENPQSNKIEAYLIAKLFFKENNLDHKTTPLKLEMSLDIQANQTAYPALEIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDG5</accession>
    <name>A0A5B0MDG5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDG5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="146" mass="16562" checksum="E7D8DAFA129473FF" modified="2019-11-13" version="1">MAPLARLQCACQRRASLAVLEAPSGAHFSKSGAVNPTRKTPSQYADRRTDPWWLPSGLSSERICWPKPWRAVLSGVHPNASQLKHNNASRVRAPKGQPRRVSVSQRGIPPTVEFRCGSRHFLGPRIVYFPLKSNSPREKLRYFYWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHU6</accession>
    <name>A0A5B0MHU6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHU6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="50" mass="5443" checksum="4992947379F67C60" modified="2019-11-13" version="1">MIEIVHQIFTAQLKPPAAQTILKHPIVPAQSDPPVAGHPPHQPVGHRKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJN5</accession>
    <name>A0A5B0MJN5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJN5"/>
    <dbReference type="OrthoDB" id="124582at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="341" mass="38203" checksum="7A7E257273055247" modified="2019-11-13" version="1">MVHDIIKNLLQVTTKGDALPNAADGRVVVSAIFKLNETWSTKLELIKSLFDAFPQSAAAAFLFSLISQFKTLGTAPDLPSSETMELCRSLSSRVFNLKRTPSKIMTEPPKYAFEKPLVVTAQDLIRFACDLHDLSTDANDLLESFILQINVHCPKFPGEGIRKVWIPFLCQLIPVLVSRSISIDTPLYQQLARQLIKYGDEKLGPAPQADPNTPRPQITCPCGDCLSLKRFLKDPHQVVGRFPLPQARRHHVYQSLDDPGFDCIRKTEHKGKPYTLIITKRLTLENKIKEWKDRRLEIYAPLAQNIHQDLLESLLGKQGAALVRSVAGVQQADARSATQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML02</accession>
    <name>A0A5B0ML02_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_017125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML02"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="308" mass="35541" checksum="D25768DB86CE8F7D" modified="2019-11-13" version="1">MNLHGPTDYPGFESRLYPDGTYKYRCVICKSKWALEHNIRRHLTCRTHVTNVATLNSFQQHVAQSSQQAHNQQGSLPHDWNDDPMEGQMELEVDYQRHQIHQALAEEQNTNRIVNVDSNDPEGFGFGHEATNFSEADLEHLGRWISDAFAAGMHDEHFLTDPDYESDGDSEAGLPESLWFPFPHKEYYVAELILGHLHHMLSRVVYDNIRVGLSILHLILPHWDSMRRTRLKIRKLLDIHIIEHETVFNNTCFSLSLKDIVAHELANPYVQLITTTQDQANRAKPQLNLISTYAQISMWLANKGNWGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML69</accession>
    <name>A0A5B0ML69_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_020685</name>
        <name evidence="6" type="ORF">PGTUg99_034981</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML69"/>
    <dbReference type="OrthoDB" id="2497308at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF1">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1076824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1126989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35584" checksum="41149DFF9CB910E1" modified="2019-11-13" version="1">MAQAIAHLEQRAFEIGADVSAVNKLHMIGRLEDNYMLDKPENASQLLFFQSATGITDSKQLKEHIISIAKEAYDVFPYPCIWALYFCKTRVITHPAYAEVLELSKKDDCQPIFLDVGSFAGIDLRQVVHTGMKPENVMGTDLIDDWAKIGNKLFNGKGVVPFVKGDIFCHSFLDRMTPPSPSAINSNFVQLDELRSLNPLKQKVRFITCNSVFHLFDEERQTELAHRLASLLNNQMSGSTIFGTHVGSDDCYLLEGTGKRGMFMHSPETWKKMWEKIYQPGQARIQVELGPPEPITHLKDFRSMTMLIWSITIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MM84</accession>
    <name>A0A5B0MM84_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Catalase immune-responsive domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_007097</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the catalase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MM84"/>
    <dbReference type="OrthoDB" id="6880011at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF9">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Catalase immune-responsive" evidence="9">
        <location>
            <begin position="80"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1077418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="16237" checksum="21551B5B091CBBD8" modified="2019-11-13" version="1">MRLFAYTDAQQYRLGANYQQIPVNCPLSTVANYQRDGASSYLGDQGNHPAFDASYAHLAVVPQAYNTDNHMIWKSGAIHYLSQPRYFCENLSPEQQKNLVSNFAGGLSQVKNQNVVQRVLQVVQQASPELAQKIYFAMSATCQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPF4</accession>
    <name>A0A5B0MPF4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_034196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPF4"/>
    <dbReference type="OrthoDB" id="19174at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="211"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1078372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="256" mass="27912" checksum="0B067C2A700C39D3" modified="2019-11-13" version="1">MSPNICGGQTERKNIWVAAGDGDLERVKELIQAGLSPTVADENSYTPLHAAASWGRLEVLKYLHSQGGDMNTTDDEGDTPLFSVEDLETAKLVIELGGDPRHKNAEGKTAAENLFEDYPEVSNYLRSVNGESPLEDRESNDPEDRLQGEETSQEDPTSSMTQTLIEKVREMMTEAESNGIDSESPELDLRLRELVTRTVDQSVGLGKQLAAGARDLSQSSPTIVSHDLATVAEGDEPPEESSQVVDQAEANKRQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQS8</accession>
    <name>A0A5B0MQS8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nuclear actin-protein involved in chromatin remodeling</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ARP5_2</name>
        <name evidence="10" type="ORF">PGT21_034675</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQS8"/>
    <dbReference type="OrthoDB" id="7340501at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000048">
        <property type="entry name" value="ARP5 actin-related protein 5 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000122">
        <property type="entry name" value="ARP5 actin-related protein 5 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.70">
        <property type="entry name" value="Actin, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="408"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="423"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="444"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1078404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="778" mass="88933" checksum="8AB7CAA22B198CEC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSR4</accession>
    <name>A0A5B0MSR4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_031077</name>
        <name evidence="3" type="ORF">PGTUg99_013780</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000444">
        <property type="protein sequence ID" value="KAA1079076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSR4"/>
    <dbReference type="OrthoDB" id="2507803at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137270" evidence="2">
        <location>
            <begin position="22"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1094834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="289" mass="28184" checksum="C556C59FED612B03" modified="2019-11-13" version="1">MLSSNFIRLACLLSIIGGAISRPQPVPQLESEEGSSSINSLAAHPHPPADTAAPPPPLDTPAPPPASDDKPPSPPGTEPGQPGARKKPGKDAKPVGPPRAPDAKPDRPPGPPGANPDAPGMDAGPPPSLDPQGPAEPVPGPGAPPPPGPMTPGKNAGLPPPPDAPGPPGKNAGPPGKNAGPPGKEAGPPGKEAGPPGKEAGQPGKNAGPVGPPPPDAEKNPLTPTKGEPANKNPEPKKPDGSRAPPDGGKKKGDEQGTGDKQSSASDLSGSVLTPGFSLLLAGIVSLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVD2</accession>
    <name>A0A5B0MVD2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVD2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8979" checksum="5AEB8D1A56482C9A" modified="2019-11-13" version="1">MAFMFRRPTNYRAFIHSAFRPPLQPRQIRNMTADHEPNETPLLPPRKKYRKAHRAAKEAQRAALRAAKGVRQTPEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWS5</accession>
    <name>A0A5B0MWS5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mitochondrial intermediate peptidase</fullName>
            <ecNumber evidence="4">3.4.24.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">OCT1_2</name>
        <name evidence="16" type="synonym">OCT1_1</name>
        <name evidence="15" type="ORF">PGT21_011179</name>
        <name evidence="16" type="ORF">PGTUg99_023900</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="16 18">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.</text>
            <dbReference type="EC" id="3.4.24.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase M3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.59" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWS5"/>
    <dbReference type="OrthoDB" id="17530at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06457">
        <property type="entry name" value="M3A_MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000055">
        <property type="entry name" value="Related to mitochondrial intermediate peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033851">
        <property type="entry name" value="M3A_MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF79">
        <property type="entry name" value="MITOCHONDRIAL INTERMEDIATE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="14">
        <location>
            <begin position="269"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1080548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1131516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="794" mass="90218" checksum="06FD159A8D413649" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWV6</accession>
    <name>A0A5B0MWV6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH APSES-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015981</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWV6"/>
    <dbReference type="OrthoDB" id="5562739at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000005">
        <property type="entry name" value="Transcriptional repressor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR XBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="2">
        <location>
            <begin position="48"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="506"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="603" mass="65101" checksum="6D9F518122C90F9A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0X5</accession>
    <name>A0A5B0N0X5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0X5"/>
    <dbReference type="OrthoDB" id="2508864at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="179" mass="20480" checksum="17E8461184625F5A" modified="2019-11-13" version="1">MLLGSALSYVLAAPVYHLIPATPVVSLPDTCPAVQFPMERPAHLQTEDILGHASSTGMHPADPHLGMDPPDYLPKEVPRKLTDEERRKIVDILRNYPPGHAYRINESPYPIRLAKAYWEKGWDGGRVRVYDENDILLHDTGYFHFNAGSILREMQKSRDKKSKRKARCRRKRKRQGKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3A8</accession>
    <name>A0A5B0N3A8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005753</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1083747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3A8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11474" checksum="37133E5F22EF824A" modified="2019-11-13" version="1">MQHSIPHVVGIKNSEIRYPVVTSEEKLARVLESDVVQRTALPLRKVAKQGINDYPQSQTCHRNAIKIFCAFEAYGLDMDEAVALIPSGTTCPLPWPSFNIIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6F5</accession>
    <name>A0A5B0N6F5_PUCGR</name>
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        <recommendedName>
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        </recommendedName>
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        <lineage>
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            <taxon>Basidiomycota</taxon>
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            <taxon>Puccinia</taxon>
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    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
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                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <dbReference type="OrthoDB" id="2496288at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0000981">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051356">
        <property type="entry name" value="SOX/SOX-like_TF"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <end position="411"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="570" mass="63341" checksum="8C1FCAB135CAB52D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7D6</accession>
    <name>A0A5B0N7D6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">PGT21_034182</name>
        <name evidence="2" type="ORF">PGTUg99_014258</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1072982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1084682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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            <end position="61"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="568"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
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        </location>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="741"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="757" mass="81094" checksum="816AF33EFEB1F02A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N830</accession>
    <name>A0A5B0N830_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N830"/>
    <dbReference type="OrthoDB" id="2505311at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="117"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="366" mass="41224" checksum="F94754645FADE0A1" modified="2019-11-13" version="1">MGISSHDLTKKIRKLDLFPEISKELAIEVEEALKDLQQYEENSKKYPDQSFPNPMDRTVLPRLPTEDEYAEALKEVQDNFRTIDHGYVRIFDAKSINIICIVMYTKLSDLDQCLRLQLDFLCEFLHGCKSFIIPPTSEDPPEGYFMSAMGWSQNMTRLKILDQYRDEKAIEANREVYDQLMAGSEKAGGTLWNMYNRLASRVAEKTMKHFKKPSGTLSADKDLPAVDANDDESTNQVAGNLAFPTNGFYADHYWDKETLPKPPTAFALVLPTSKSTGQIKSKSEGYQVDHGQLVFPDVKIALTFPPDTVCEIVFQPHVYAHGTMKAKAFGDSSRLGICIQAAPSSTYVVKKLLEVPPNEETSASEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAY8</accession>
    <name>A0A5B0NAY8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022813</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NAY8"/>
    <dbReference type="OrthoDB" id="2509775at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="93" mass="10766" checksum="48B6A3736BFF133C" modified="2019-11-13" version="1">MMEIEPPQVKDTPHQRAVINVQNAARVQEMQAAISNRTQELHILPELVPNVLGIWNMQRMKYGRDPIRIRNIENLTNIPPKSTTTNNQRKIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBZ1</accession>
    <name>A0A5B0NBZ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBZ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="10495" checksum="828EF13AB63BC70F" modified="2019-11-13" version="1">MLPGRRQIDKPDGQSACLRASLGQPQRAASRRASVQSGADSLLSASSNLPPKLTPTSRKSSNVSLPSSPKASKPSTSSTKTTNFKPCGIAPLEIDLPTIPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDQ6</accession>
    <name>A0A5B0NDQ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022598</name>
        <name evidence="3" type="ORF">PGTUg99_003514</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDQ6"/>
    <dbReference type="OrthoDB" id="2020070at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33604">
        <property type="entry name" value="OSJNBA0004B13.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33604:SF3">
        <property type="entry name" value="OSJNBA0004B13.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="623"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1125" mass="125925" checksum="5CAD3ACD3C22A3B1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NE53</accession>
    <name>A0A5B0NE53_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_032737</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NE53"/>
    <dbReference type="OrthoDB" id="10266980at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF0">
        <property type="entry name" value="TOPOISOMERASE I DAMAGE AFFECTED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="56"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1087507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="277" mass="31682" checksum="79896237AEDA09D5" modified="2019-11-13" version="1">MKNINRLAAILDLENLPEHYHVILLSTLACFLIQAISHSLISPRLFPTHYPQLSRFTKFNWDTRVVAWIHAFYATLIACYVLRNPALFSSIHQDKLFGYHPTAMNYLSISTGYFLWDIIVSLKLTINRGGIGFLLHAISCFVAFLYSIKPFCGYFGFAFLLWEASTIFLNPHWFFDKIGMSGSKAQLFNGVALLLVFFFSRLVLGNYTSYQLFALSFEDGVSQKAGTRLLNTIRILDLLLSGLNMFWFYQMISSVLNRFSANPSPPKPSKPSDRKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEV7</accession>
    <name>A0A5B0NEV7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEV7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1087293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="64" mass="7247" checksum="7E6169F871B40942" modified="2019-11-13" version="1">MIGIERLNTLSSVYEQLDALLSSPSPPQSIYVQSASNLDFTLELLQQLLRNSSNQLPHLDLRMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFH6</accession>
    <name>A0A5B0NFH6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFH6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022745935" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="12204" checksum="45BCA080A65E0057" modified="2019-11-13" version="1">MLCNTVIALSFAFLHSVFAESVSPRGFSDILGGLPTDNLLGGGSQPGGDMFSSLGFGGQPSETSCADQSALLLNAQVLGQTRCSGGGGGGSMSCNGQNGGKFGPVTLPSLANVQALTTVDCTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFN3</accession>
    <name>A0A5B0NFN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFN3"/>
    <dbReference type="OrthoDB" id="199930at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="107" mass="12128" checksum="10214B4033302805" modified="2019-11-13" version="1">MQLVDIMMTAFLGNKNPSNSVIEGLALLSEEESAREILRKRSEEFFQTTQSLSQTNRAQENLTSAQSMASGLATIILNLKPLVIPYELRFDNGRRRRVQASTYHTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIY0</accession>
    <name>A0A5B0NIY0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIY0"/>
    <dbReference type="OrthoDB" id="10413477at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="202" mass="22120" checksum="C8A33F947D6EE248" modified="2019-11-13" version="1">MPTSKLPSPSVSTVDNTKPPTVPLTQPNHPDSPSSPSPINSTLPLPALPDCSIPIYPTAPACPVNLEIAAIGSLTIENDDIDSITARIAPEYSQATLDYYNDIQEYLQQANADETETKKKKKKKKKKKANPTSTPNNPILFYTAEIFLSCLVHKSQSVCNTQRGSVQTRPGNPVPKQNFEDNRLIQLKRKGKPRSGLAEEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJC5</accession>
    <name>A0A5B0NJC5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJC5"/>
    <dbReference type="OrthoDB" id="2500735at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="283"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="757"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="760"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="773"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="782" mass="87804" checksum="D2E81E922F7DF107" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKC8</accession>
    <name>A0A5B0NKC8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable electron transfer flavoprotein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_001463</name>
        <name evidence="9" type="ORF">PGT21_001617</name>
        <name evidence="8" type="ORF">PGTUg99_008654</name>
        <name evidence="11" type="ORF">PGTUg99_034040</name>
    </gene>
    <organism evidence="8 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </cofactor>
        <text evidence="6">Binds 1 AMP per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="6">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000204">
        <property type="protein sequence ID" value="KAA1124488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKC8"/>
    <dbReference type="OMA" id="EINQPRI"/>
    <dbReference type="OrthoDB" id="276685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000349">
        <property type="entry name" value="Electron transfer flavoprotein beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="7">
        <location>
            <begin position="31"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1088299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1105285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1114241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1124488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="271" mass="29797" checksum="D6581472B78B2C77" modified="2019-11-13" version="1">MRASLVQQLRVLVPIKRTIDYAVKIRVNSAKTAVETNGVKHSMNPFDEIAVEEAIRLRELMKEKIESITLLTVTSQKKGGQEVLRTGLAMGADEGILVEWPEGSPEPEPLSVSKTIKAVLDKQFKDKPIDLIICGKQAIDDDCSQVGGMLSGLLDCSLAQFISQVKFEDQSNLKQIKIEREIDGGIEKLTVPLPAVLTTDLRLNEPRYASLPNIMKAKKKPITTYTADQLGINTKPMLKVLKVEEPPARKGGQKVDDVDAMVAKLKEVGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLR6</accession>
    <name>A0A5B0NLR6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018664</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLR6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="131" mass="14922" checksum="0438A92CB1493083" modified="2019-11-13" version="1">MKERKNITSGCQAADEGRGLPRYVDWYHNTGLKVLIFRIQDRKYACLGSAARIPNFEYHVFSRDRHSITIRDIGVYQIQIPPNTDTRLISVAALLVVLLVGTARLPEILPGDLVETNQVAKEGPYWRPDWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NR33</accession>
    <name>A0A5B0NR33_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sec61p translocation complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SSS1</name>
        <name evidence="11" type="synonym">SSS1_2</name>
        <name evidence="12" type="ORF">PGT21_006914</name>
        <name evidence="11" type="ORF">PGTUg99_028242</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="11 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NR33"/>
    <dbReference type="OMA" id="KPDQKEY"/>
    <dbReference type="OrthoDB" id="2401875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1074174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1090610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="69" mass="7699" checksum="EC06440C228E21FE" modified="2019-11-13" version="1">MDQVKELGEIPQKFLKEGSQFMNRCTKPDQKEFIQICRAVAIGFAIMGFIGYFVKLIHIPINGILVGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSK1</accession>
    <name>A0A5B0NSK1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NSK1"/>
    <dbReference type="OrthoDB" id="268763at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="1">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="67" mass="7624" checksum="CE209A97E75F7DF6" modified="2019-11-13" version="1">MKRFDPTSNSGETRWQELHKRVVEHVSLLSELLDLTIPESEATLAKLVSSKTVFAKIDRPSGIVRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSN3</accession>
    <name>A0A5B0NSN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011687</name>
        <name evidence="2" type="ORF">PGTUg99_014340</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000380">
        <property type="protein sequence ID" value="KAA1091923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NSN3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="172" mass="19084" checksum="411DDEFD96360699" modified="2019-11-13" version="1">MTSFLFEYVCSSPTLTLARATSATHPETLTQPFLDVCATGPPHCHCRYGTHIGMTAPDNYISFKVQFELLIHNSKKVIHCQCLQSTSTNQERRLFSSRVERMGQLGFRSGPRRSGKIRKTRKPDQGLGSTQRDLASGRILKTPRSRGFAGITRIKPAWGPISCCTSAPTFPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSS2</accession>
    <name>A0A5B0NSS2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_025211</name>
        <name evidence="12" type="ORF">PGTUg99_009567</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NSS2"/>
    <dbReference type="OrthoDB" id="5327538at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072354">
        <property type="term" value="F:histone H3T3 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024604">
        <property type="entry name" value="GSG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419:SF18">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HASPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12330">
        <property type="entry name" value="Haspin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01331">
        <property type="entry name" value="DUF3635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine/threonine-protein kinase haspin C-terminal" evidence="10">
        <location>
            <begin position="436"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="493"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="589"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="503"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="589"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1084350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1092261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="728" mass="82109" checksum="92517017821BD013" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUF5</accession>
    <name>A0A5B0NUF5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUF5"/>
    <dbReference type="OrthoDB" id="10291462at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022727352" description="Secreted protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15483" checksum="A4C2A4CF711582B9" modified="2019-11-13" version="1">MSIMNSFQKIFITFALVGLIIGLLSGQAAAREVQCDYHFAPLDGVNTGKGSCISSANTAQDNYCSLDYCGVRATPTTYTHWNNVQYIQCEGLAKVFVQQYFRYTTYVSAQDKFNGKFYKCYYQPPQNTYYISCTKCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWU5</accession>
    <name>A0A5B0NWU5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011778</name>
        <name evidence="2" type="ORF">PGTUg99_030368</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWU5"/>
    <dbReference type="OrthoDB" id="10350877at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1092707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="395" mass="44760" checksum="6BFF886A40D35F9B" modified="2019-11-13" version="1">MMSQLTEKPTATATLQSQQLPHQADLVIQGFRRLIAEYDLSGSAGNTTDLSLDHPLRSKEDILAELGSSLLPLLKQQITSLSQALDNPDQLRQDLGPTLQLILELQPKLAQTLYRIIRAIDHLVPPEGPESSPTIDQDMKELKHYRLRRLDTSIRVHLRAEIRHFFLASCRIIEAIKLPTQQRSTPIGYLLFGISDPIDRAVRWIEASEFLLLQNWWLRALGDIEDAVEDLLGVANPTNRQTRGPLPQLARSSIPLLKLIRLFFNKFFDLGLAKEELVKSYTDMSTEQLLSFEKFAQDIAEAILGLVCGLQEEEDEVVDQAQISLDFIHRVKSLSPLFQSLLLLSNLYIIPLFPDSDLSPSQISFKPWLVTWYTLFFQATNNAIKTANLLSQDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1A7</accession>
    <name>A0A5B0P1A7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7918 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_032997</name>
        <name evidence="3" type="ORF">PGTUg99_007955</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1A7"/>
    <dbReference type="OrthoDB" id="3237202at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057678">
        <property type="entry name" value="DUF7918"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223:SF5">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25534">
        <property type="entry name" value="DUF7918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="118"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1094933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="426" mass="47907" checksum="E00E4E471D3890EF" modified="2019-11-13" version="1">MPTNSASAARCTIKLITPPSSTSTKPTKTACQEYSQQSIKDPITGAFKEIVTIESKQSSRFQISFQVHHTAYDLLERTKPTCSSSTETDRALKSSDYLVEVLLDGISIGGYHQHRSQTPRPHNLNAFRSSETTTRLLEFAPLHLVDPDDFVQVLDHVAGNPTPEQMICQDEQIIRSLGTIELKITRCELSMKRRAPSIKQIRSRQTTNQMHFSEAIKKTRLSSTVGLGLPTLDERGKDKVSYRVNSKDRNPFLHFIFHYKPRSILIAEGILPASPIIPTELPGGGSSTEKQELERRSNWPETRITIDSSASESEKRESKADKINKKLEKKTAVDEKNEKEKVTDQLASGRRRKFDNCKKRGDEEEEEGNGEMDHQEKRGRVSPKTDQKPANPSTQTKLIINPSEPLINIDQDIKPAQPAILIDLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P583</accession>
    <name>A0A5B0P583_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P583"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="620"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="629"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="640" mass="69807" checksum="D8CA477B8EFD08AD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P6V5</accession>
    <name>A0A5B0P6V5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">VWFC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012527</name>
        <name evidence="3" type="ORF">PGTUg99_037235</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P6V5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366369" description="VWFC domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="9982" checksum="8B43C4B4E1145A4E" modified="2019-11-13" version="1">MRSTLAILLVSLVLCHISIAQTCSKPGKCTCTSIEPPRPAPENYCPEGCCVQPPVAQTCSKPSKCTCTSIEPPRPEPEDYCPEGCCVGPPSESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P771</accession>
    <name>A0A5B0P771_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031662</name>
        <name evidence="2" type="ORF">PGTUg99_013412</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P771"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="84" mass="9955" checksum="E6166A4E0519071D" modified="2019-11-13" version="1">MRIQIRRGWEPSISSLGDTDQRINRVWRGCLLSLDFTVLWWTFVSWPCPSTCLKRKHFLILNKNKPRRSFWTNGNQHPAVAKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7R1</accession>
    <name>A0A5B0P7R1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008720</name>
        <name evidence="3" type="ORF">PGTUg99_029672</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7R1"/>
    <dbReference type="OrthoDB" id="2504990at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="386" mass="43120" checksum="0A5AF184E96A2E38" modified="2019-11-13" version="1">MAARLPDTHRRHEEQAYQDDLSRILASRAFPNTTSTIPSLLPFETNFQGNMNGPSHPTVLIPVGKPRELPDQAVMVLRGLWARLGYQANSFVEMQLEITKELVHADFAKFEREMLCNEVISLRQELEMIRVDRNEHIRRAEVAQAKLAQAIDSEERTFKALVAEREIAKRAIEQAEKGVQQVVIAKEKEAVRAGAMSEEIHRLEKERLAAVLYARTAEEQAAMANITAKTAAAVAMSAVQSQAGTPRVGFAPSPAARSPATLPLANPICQPHAVRPTTPTGSLHPHHRSSTPRSSVLGSPHPTSPRPHTPHLSSPMSRLNTKNWSETDEAILQSKIRERNIEAEENRLAAEVTRLAEDRIQLRFDRDRLASPLHHTPKVTYQNLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCU1</accession>
    <name>A0A5B0PCU1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCU1"/>
    <dbReference type="OrthoDB" id="2512332at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022745420" evidence="1">
        <location>
            <begin position="25"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="344" mass="38993" checksum="3B16EB566E10FCCA" modified="2019-11-13" version="1">MLCSKSPFLFVALYILQAVQLALSAEEVLGIENVTPQIMNPKSTNCVNIIDTPGSLSEHSKHENLGEAMHGVISTPVDLASEKQDSLDKSMEDRVKNLIKNLKANVMEELEAVINHLQQFHNYLASSIEESEKTIKDPQNLVKSKPSEKENLFDGYEDSKITVVPIGSAINLNMKLSSTTYVKDIIEAKVIPIWKYLQAKSIGLKYEPQEKEPETKLNFLKSLFNLGDYIYKNDLLSQEEMKEIEIFKPSIVVKLFKLNIDLLISTKGKDLFGSPHWVAPHIKQLIKNPELKHMQRSIKGLGEQHQSFTEYLTLKSIIANHRLLCPDGYDDYAGLISFWVHWGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PD84</accession>
    <name>A0A5B0PD84_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SigF-like NTF2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032775</name>
        <name evidence="3" type="ORF">PGTUg99_005385</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000086">
        <property type="protein sequence ID" value="KAA1132268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PD84"/>
    <dbReference type="OrthoDB" id="2344312at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057514">
        <property type="entry name" value="NTF2_SigF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35393">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35393:SF1">
        <property type="entry name" value="SNOAL-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24840">
        <property type="entry name" value="NTF2_SigF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SigF-like NTF2-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="190" mass="21520" checksum="8E301ABDEF2D665F" modified="2019-11-13" version="1">MENPRAEIADVVRSITEPQDPETVLKNIDKYFTEDAFILHPMANQPELARGRDNLKALYYMFRKGTKDNKIHFHAVMFSEDLTQCTLDLTEDVLDVKSALFPSLATTHLSLRFLVRVDLRLESDGKYRIWRQHDNFVSDLGMSGFKLFPGAGFISDLLKSSGALFTIAIGRYLAGNMISAAERNPCCLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEB3</accession>
    <name>A0A5B0PEB3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007510</name>
        <name evidence="3" type="ORF">PGTUg99_010933</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000343">
        <property type="protein sequence ID" value="KAA1099062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEB3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="147" mass="16574" checksum="2E903B6C02CD9F24" modified="2019-11-13" version="1">MDADGWMISTENETSKKLPVCDRKTGVMNQDEPAFWSAGLSDQLTSYDLRAVTWQELRACLHNNHGRRKNHDLPVLRERDPLPPDVAGKAASVDPTPKSTTTSVFLSDRQDWGGSYHDYTHKYVIPDLGAYSEDSAQTPSTRVHWDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEV8</accession>
    <name>A0A5B0PEV8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_002803</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004518">
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:mRNA catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Unplaced genomic scaffold supercont1.17, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF2">
        <property type="entry name" value="STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017179">
        <property type="entry name" value="RISC-Tudor-SN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TNase-like" evidence="6">
        <location>
            <begin position="77"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="6">
        <location>
            <begin position="271"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="6">
        <location>
            <begin position="443"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="5">
        <location>
            <begin position="659"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="235"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="291"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
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        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="406"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1099302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="841" mass="92651" checksum="6EDC617DAC6C6052" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PG50</accession>
    <name>A0A5B0PG50_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_020633</name>
        <name evidence="3" type="ORF">PGTUg99_032080</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PG50"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
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        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102:SF2">
        <property type="entry name" value="GAF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102">
        <property type="entry name" value="SLR1143 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="338"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="555"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1095457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1099792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="752" mass="83044" checksum="DC300C32185A4B96" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHC2</accession>
    <name>A0A5B0PHC2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHC2"/>
    <dbReference type="OrthoDB" id="2497276at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="251"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="364" mass="40692" checksum="321EE0DF6A8B6492" modified="2019-11-13" version="1">MTQSNEEDPDPTTDELDPTPTPELYPDQTPECDSDSTPDSTPDIDVSNPTPDDTPAERLPEDATENTQAQGKRMTSVNLEAHMIDYEGFGYSNGDIINNKNLSRFQHIAAVVCSMVCFARNRRQNGMQLTNSIRFDGCGVSETVNEHLHYMGITSSRKTAIQALRSLARHAQDAVRESMALTQDFAPLLCIDNLDMEERVQMSTIGHQSRMFHGTWGYIHIPSKDLWSTLNPVELSLEAYHNSLRTASSMVIDPEFFMPTDSPNDDYKAVWKSQIAQVMLKYVAKPSDRQAIIPLDPPPIDPISHEAPSIHMLKLMDESDNSAEGIGQVMEALRRQSGLEPEEFFGRLQLMEGDLGTCQIFNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PL24</accession>
    <name>A0A5B0PL24_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027418</name>
        <name evidence="3" type="ORF">PGTUg99_021102</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1101673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1136298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PL24"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1136298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="624" mass="72264" checksum="558BB24735F02618" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PL81</accession>
    <name>A0A5B0PL81_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051087">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43948">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43948:SF10">
        <property type="entry name" value="MRJ, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
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    </dbReference>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
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        <location>
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            <end position="208"/>
        </location>
    </feature>
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        <location>
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            <end position="272"/>
        </location>
    </feature>
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        <location>
            <begin position="294"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
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            <end position="157"/>
        </location>
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            <end position="178"/>
        </location>
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            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
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            <end position="241"/>
        </location>
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            <end position="271"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="557" mass="61744" checksum="1639E0E0D0BA60A5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLK0</accession>
    <name>A0A5B0PLK0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000044">
        <property type="protein sequence ID" value="KAA1102487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLK0"/>
    <dbReference type="OrthoDB" id="2507084at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="507" mass="56871" checksum="026CD0F8C5EACB1D" modified="2019-11-13" version="1">MEDTQMQDKPDESSETPSSVFDDDSSESDEVDADVLESHTSRKRKNRRAKKKALEERNKISKIVKSGPLPHQAGALSSSVTQFIRHMLGMANSKSPLPSPPSKSEIAQWANLVDARRETLLKKVNANRHEPQDLDADEEEPVEVSRSTKKKQGGSAAANDKFLRKQRLAALAKEGVKLVEYTPAPEIPPEARLQPISFQTKTNVDREFQAKGFSRITFEWSARSLSASRWNVATASLLIEHWSRWYKTQMHDQKDLEQNILGVVERWLGTMRTNYSKQQRTEESSGSAPSTSQGQEGAPRAVTASDRTKVKTRKKVNFINFYYVSDYEDNISEPTKPRTRIIPRWRNEIFTEVVHQLDEAAKQIQKSPSSERKVANLLRRGDTQIEPSDAEEPMEAPTGLPADCYHPNFVENIPILRRRHLKRSEPIGLEKFLETLKSLTIPGYKNADEQNTAGSSNPNNQTPNPGISGGNVFTVSSIHLNMGQINNPAVSGNMNSTPMPGNFTTNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMD2</accession>
    <name>A0A5B0PMD2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_028636</name>
        <name evidence="3" type="ORF">PGT21_029358</name>
        <name evidence="5" type="ORF">PGTUg99_028968</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PMD2"/>
    <dbReference type="OrthoDB" id="2507380at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137809" description="Secreted protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1101728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1116890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="17691" checksum="712CA7D118F70A3B" modified="2019-11-13" version="1">MVSLFLQTLLAAMLVSVMPSTKAIPTTVTSSSSSSSSQGATYINGQQVGGHCEASKNGYSSGCQDPNFNAIPDTITTVTPGDFMPFGFPPLPNMDNFANNFQFPNFANGFPFNNFPDFSFPPFNPISSFNNNFNAPGSWNNPAGFNGAGSPNPGPPKPQSPKPASPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQX7</accession>
    <name>A0A5B0PQX7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQX7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="205" mass="21864" checksum="3B2C024048F67F06" modified="2019-11-13" version="1">MDTRPYLTSWARSRSPTLSSGPGEPYPSPCWQRLHTSSASDWDQVARPPPYTSRTDDAAAVYPNASVHLTAISLLAWPALPTLRPSQTGDMPPSCLDSETLCIGPNQLTCFPSCSLNSKVDKQLAIMPLAFLCLLRVKGLTFLLLSLAASANLNSKANKQLAAPPLPASAPSQTTHCPVRLSSSAATANLLSDSLATLSRLQVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUD0</accession>
    <name>A0A5B0PUD0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">NEO1_1</name>
        <name evidence="20" type="ORF">PGT21_016016</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000097">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000254">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="540"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1079"/>
            <end position="1099"/>
        </location>
    </feature>
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    <sequence length="1281" mass="143894" checksum="C4FE8F267136AE59" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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                <person name="Schwessinger B."/>
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                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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    <sequence length="76" mass="7847" checksum="517160B2336C7DDD" modified="2019-11-13" version="1">MEDSNADIQVSPPAPSQASTPAPSQASTPAPSQASTRRKRTINSSTDDGDGSASFTPVPESESQDDAPQSQMKKEG</sequence>
</entry>
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    <accession>A0A5B0PZL1</accession>
    <name>A0A5B0PZL1_PUCGR</name>
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        <recommendedName>
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        </lineage>
    </organism>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
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        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL35 family.</text>
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    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1106326.1"/>
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    <dbReference type="OrthoDB" id="162638at2759"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.60:FF:000001">
        <property type="entry name" value="50S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00514">
        <property type="entry name" value="Ribosomal_bL35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001706">
        <property type="entry name" value="Ribosomal_bL35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021137">
        <property type="entry name" value="Ribosomal_bL35-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018265">
        <property type="entry name" value="Ribosomal_bL35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037229">
        <property type="entry name" value="Ribosomal_bL35_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00001">
        <property type="entry name" value="rpmI_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33343">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN BL35M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33343:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL35M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01632">
        <property type="entry name" value="Ribosomal_L35p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00064">
        <property type="entry name" value="RIBOSOMALL35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143034">
        <property type="entry name" value="L35p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00936">
        <property type="entry name" value="RIBOSOMAL_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1106326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1109382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="189" mass="21579" checksum="4EA0BA200174A4A2" modified="2019-11-13" version="1">MNILRQLILRTIKPSLSSYQSVPLKPIHPFRIQQQQQQQQQQRAFSISPSTHWKSIGTPKKNRKTRCKLKTHQGASKRFFVTGRGQFKRVQAGTQHLMTGLSQTRKRKLKPMVIVKRSQTSLLRKLLPYAGKRAGFRKLDQSETVWWRSGKLQKSGVALKQAIQRSHALKRSQLLSAPSSSPATTTISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q3C2</accession>
    <name>A0A5B0Q3C2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytidyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q3C2"/>
    <dbReference type="OrthoDB" id="330671at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="1">
        <location>
            <begin position="141"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="336" mass="37524" checksum="AEA9A20140F324BF" modified="2019-11-13" version="1">MAANERSSPRRVARVHCLMELARTTADQEDLLPARFKTEVAGEMSGLSGRDKLEVVLSLGSSSSSQPADTRRRLETKRLERILCLIYVEIERHKALLDQLLIDVEVLIDGFSQRDRADSAFFSPSSSTLETEDGLFNTLALGGTFDHLHSGHKILLTMAAWLTTHRLIVGITGQSLVSSILPFLPNIYNEEKEPPPPPLFPPRLTHQCFRNWMETTDDELLKNKKHASQLEPIEVRQEAVRGFVERVGPSDLRTETPRLQDVYGPTATDPTIDGLLVSQETLAGAKAIDAIRSQNGLKSLRTYVIDLISNQLTLAQLDQLNLKISSTQIRAWLDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5T3</accession>
    <name>A0A5B0Q5T3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5T3"/>
    <dbReference type="OrthoDB" id="10605275at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="133" mass="14759" checksum="5F6C1EBD6150C65B" modified="2019-11-13" version="1">MPPRTTAANTPLTDHFYGSSESPANDDLPTYSPGSNEQGDLPSLEGLLPEIHPALPLNSAPAQPFSVNIKWAIIFMVYCPDRNKTTKKVTWVSIQSPKKLSFEFSSGNMDWPTFNTAQRLHHSRICLKSLKMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCI6</accession>
    <name>A0A5B0QCI6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_030055</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCI6"/>
    <dbReference type="OrthoDB" id="408493at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012404">
        <property type="entry name" value="UCP036436"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036436">
        <property type="entry name" value="UCP036436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="84"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1110704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="50719" checksum="6E86CCD974E5E117" modified="2019-11-13" version="1">MSNSASAAILVTGMLITGCSNSLWSKFQDQQCVENCANPDPTTHRNFEQPVWQTLNMFVGEACCMIAYYIIQWITRPTRDDKELGYAPIPRDSMDGRLQEEEEQESTLGDTLTSDHSASLSDSLLQPAHLKSRLCPNPTPLAVHGPSPAPLASSLEPELLVNQHEPSVLQLVGLQKFVFWLPALFDICGTTLMNAGLLFVPVSVFQMIRGALPIWVGLFSIIFLNRHLSREKWISLAIITSGVALVGYAGSLQPQPLQPSDQIENHLVSLAAINSSEPGGKVVLGLFLIFFAQLFTASQFVIEEKIMCKYEVPPLEAVGLEGVFGLITTIVGIPILHIFIGSRPEGRGGYFDARTGVEEVLSNPRIMWSSVAIAISIALFNFCGLAVTKSISATARSVIDTCRSVGIWAFSLAIGWESFSFLQLVGFIILVVGTFFFNQIVHYPTWFKRLIGSPPSEQLRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QH76</accession>
    <name>A0A5B0QH76_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QH76"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="139" mass="15066" checksum="EB07E28285C84521" modified="2019-11-13" version="1">MVLNAAPVWLNAAESKIGLPNGKVNCTEERANETFDYQVFFCALDPCPRRFSLRFTRATNAFVDEADRQHNPKEVSAALVATSCASSSVPQISRERNRNLTPASRPVSTPEPPPPFNLAPTTGPAGAKVKALIMPKVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QH88</accession>
    <name>A0A5B0QH88_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QH88"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="107" mass="11819" checksum="B1FFDD560683070F" modified="2019-11-13" version="1">MRLCFSMYSFSIVFLLVGQGGCFVWALKKKSFNEGSVVPDEASEKKKKTGGELMLRVKSLREIDQKKGVFKSIQIVVGRCNTSLKTTAGNRPLTDISLTKKSPIVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHF7</accession>
    <name>A0A5B0QHF7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHF7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="294" mass="32466" checksum="4A97F460C8225931" modified="2019-11-13" version="1">MDRSDYILSTRLRDLIPAPGGILLEGLPKQEHRLTGGLPGGYFFLGFGTWAGSWGNFETPYCCKGNQFGNRILLPLRGYPSTPPRAATSSPNLNPRHRISISTASLDRWFIHLEFDSTITSVTTGPWQAPIYVGACQGPVTTRPIPQINLDFDLTTVLHAPLTSSMARWCSFSFRIVIAVVIGLIGQPHSKSIKRYDENPLRYLSSSAFFPSQRLHSISKHPLFHRVVFLSCTSSFTTTTMSPETSNMSSPVEIPAPRSYQAISNPTPVNDVSSGRSFPARGNWSPLLCPTLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ29</accession>
    <name>A0A5B0QJ29_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ29"/>
    <dbReference type="OrthoDB" id="2508433at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="447" mass="52261" checksum="91B85D244194CECA" modified="2019-11-13" version="1">MKLYLDLTQNSPRFLGGSGRLSIAILSDHHNIFVKLVNLREFVSVHSPHNVPYSTDVPFALVRSLEVKGGFVFRVFDRSNGRVQGAHTMAGFHYNLIKWLYRVHERMLNELDIQTYVLYSQQKKLFAWLHDLIFTPLEGAPIMGLKISARPKWEPEDTPGPAKLKLLEFFAQHKNEEQVSFLTAFDLIDLFYKHHPLPGRPLEQPRKIPVDPYIEARVQFFLKLDEDQDAKYQNLFKKSRIQPQDDHLIRSSIELFEKKNDIPNLKSQTLSIHPRLLISIYYLQETPEYGFLLVLKQNDVELFRCREMSIAYKRLLRAMNYIHLAILDRMNLDSPERYERRKALFQWLHKNVVEPQTGIPIYGKIKLNVPNLAPWEDGSYRDEELFTPVQVELMEYLSSQNNPVNLKAHAASIFTAWYQLHFSSEFPTLVETVNQQSQDPRMAHSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJJ8</accession>
    <name>A0A5B0QJJ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphatidylinositol-binding protein scs2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SCS2_4</name>
        <name evidence="9" type="ORF">PGT21_029342</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJJ8"/>
    <dbReference type="OMA" id="GFKRPFN"/>
    <dbReference type="OrthoDB" id="264603at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160219">
        <property type="term" value="C:cortical endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140506">
        <property type="term" value="F:endoplasmic reticulum-autophagosome adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160214">
        <property type="term" value="F:endoplasmic reticulum-plasma membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033149">
        <property type="term" value="F:FFAT motif binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051685">
        <property type="term" value="P:maintenance of ER location"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902647">
        <property type="term" value="P:negative regulation of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000813">
        <property type="entry name" value="Vesicle-associated protein 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
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                <person name="Nguyen-Phuc H."/>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
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                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">21-0</strain>
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        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0QRG8_PUCGR</name>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
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                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
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                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">21-0</strain>
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    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <property type="entry name" value="SPATS2-like"/>
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        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED SERINE-RICH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15623">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED SERINE-RICH PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
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            <end position="201"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1039" mass="108062" checksum="7900FCA31D2F2761" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QSK3</accession>
    <name>A0A5B0QSK3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005518</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QSK3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="118" mass="12957" checksum="7B6401AF5011BFFC" modified="2019-11-13" version="1">MPGNHQIHRIGHANVQSWLASRTFGGSLREVHGTLGSPQPSGARGLCEAGNNSWGDALLCGVKNVQPNRFGLESWNSHFDWIKKRSGLRGLRLKYVPRKGLVDEPLVERECVTGATEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTT9</accession>
    <name>A0A5B0QTT9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_023943</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTT9"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="92"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1116717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="343" mass="37788" checksum="13E7D2CBEE28C064" modified="2019-11-13" version="1">MMSFIRSYPNLIPKLLAHFNSSPIVNVLMRIIQSEQTTDGTIDWLINSTDFVELIIGLLHPSRTPDLHRSVAEFLKDVIAFCTNHVISTSPSPNLPNKVSNGSLPGAEGGPTSSPDLACPVPGPSIQQSSDDSEDHPKFITTRLMRELYSSAVVSKLLSFGLGTPVSEDDGVITNTSLASSLINSLSVVIDLIRRNNSDYSEHQLMVYLRDYPPAPRLDNLHFVDAILYSDPRVLDTVQRIGNNTKADKKVAEGGHVACRMSHRLAIGAADADDNKITLILKDDVDIEAAFKYLAGTILRDVPKDWDIIFFGHTDFSNKARNGSDPSTHNFYIYKSVKPQGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QUD7</accession>
    <name>A0A5B0QUD7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QUD7"/>
    <dbReference type="OrthoDB" id="2504869at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039191">
        <property type="entry name" value="Nopp140-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216:SF1">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="693"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="804" mass="91068" checksum="11402BF229FCF050" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX49</accession>
    <name>A0A5B0QX49_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026666</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QX49"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="137" mass="15271" checksum="3176D9CA55CC7A1B" modified="2019-11-13" version="1">MNYQHIVRSKKPTLNIATVVRSKQPTLNIINSNLISYLKPNPISSPSITLSLFPFSSSHLVIRLLFLIPDSFLPANQPQPHSLSILKNITINTSVKFVELSLKTHQLPHSSTSSIFPSSTSSLIDFVNLLLHWITSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXU6</accession>
    <name>A0A5B0QXU6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ubiquitin-conjugating enzyme E2 2</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">UBC2_2</name>
        <name evidence="21" type="ORF">PGT21_022346</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXU6"/>
    <dbReference type="OrthoDB" id="9984419at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000007">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0749">Sporulation</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="20">
        <location>
            <begin position="158"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="18">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1117753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="320" mass="35266" checksum="608F66A79F8DD0AD" modified="2019-11-13" version="1">MTASIHWNCNFPSQLGIGELFVASLGQVCCLGSQIYKLWDGDLQCPKLSGSWRLQTDGDFHLCLPKLSPNLSGDCGRMILDGGRRMGQDGVLLTDSQAERTQPAESLKFWGTRQPTTSSIQQPTTLHLTTIINTIISLHINRTTSPQAQEGKYKMSTASRRRLIRDFKRLSSDPPGGISGAPCPDNIMIWNAVIFGPADTPFEDGTFRLILTFDESYPNKPPTVKFLSKMFHPNVYANGELCLDILQNRWSPTYDVAAILTSVQSLLHDPNPNSPANAEAANLYRDNMKDYIKKVRATVEASWIDEGTIPGTANASSTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QY54</accession>
    <name>A0A5B0QY54_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QY54"/>
    <dbReference type="OrthoDB" id="2518661at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="111"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="214" mass="23877" checksum="5A91E465698E654D" modified="2019-11-13" version="1">MRREHIKLDLYPNYPPLDNTRPGQRATHPTPADFEAAEELILKEFSLISYGPLSLYERPPKADPNTNPEGDPSPPEPSGLTNESGMPPKPEKPKCTFIAYIQFTRSEDLSEEDKNNLDFITTFLHDSTQFVNPVDLIHKIFRGKMWTIGWCKSQTRGEVAGRYIDTAGIPQNLCAYLDNLIDGISASDIIHKLFYSIADAAVDAANVLLTSLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYP0</accession>
    <name>A0A5B0QYP0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYP0"/>
    <dbReference type="OrthoDB" id="2507159at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="511" mass="56921" checksum="3FCA7FFB93EA84C6" modified="2019-11-13" version="1">MNPHHQRTPNSNLVPRSSEITPLTAGHQFSPQHLPPHNVQNSPHSQQSSQAGQHSHYASLQSSQSPGHYNTLNQPLFHPPSPDVFTPTPITPFEHHRARLQSTPHPTGRSTRDQVVFLPILSSNSSSSSSHQSLNPHLNTRRASHINRSDSSCSYASTPGAPIPHQVLRQRVAPLHPSPLSSSTIQSSTCQPSTLTTPPSRVNQPRPGNQDLTSTSSPVPPPPLAGLHDRISKKRKCSEISEDGGPEAQSEEELLKLTEAQLAIQARKASKRAMSDDDRDFFANFHHRQRKLLIIQAIERGVSMPMVDGYLGKRMVIKKPSGWNRFMQTPKVREVFRGAQKGVKDKSAMPLVSTMWNELPEEERLRYMKPSSKKQHNNLDPSIAATNNENPSGEADDDNEDELQVIMRRSVSFKRASDQVQDFMADWFEQAVHISKTCDCEMVMFAVSRHLGTHSFQFTRSTHGATRFVMGAQILDGTRHYAARFQSYITGYEVAQIAKINGKKQGKGPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R084</accession>
    <name>A0A5B0R084_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R084"/>
    <dbReference type="OrthoDB" id="10369827at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="104" mass="11396" checksum="B37B42E66ED3161B" modified="2019-11-13" version="1">MVPFKNYCGRQLTCRCGSPIGHKQCEELKEGTHQTCEAGCGWRDDEYVKLCTDQDHHQEPCKAFLTNPGSFETPSSSSTSRSGSFSSETTSHDTAESVQSVCSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1A9</accession>
    <name>A0A5B0R1A9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1A9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1118704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="14436" checksum="EFBB50E8741012FA" modified="2019-11-13" version="1">MVLFELLINRLFLYLEEETFQQSETTNDLSGLGGPFGNEKKVLECFHGVESSFVVTCKGSSKWDNDVPEDISGILLTFDKKTKKFVLLALDSPDYFSFKSIEILIDDLPDDDDLLVFGLDFIGGGNRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1B8</accession>
    <name>A0A5B0R1B8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011965</name>
        <name evidence="4" type="ORF">PGT21_023256</name>
        <name evidence="2" type="ORF">PGTUg99_017631</name>
        <name evidence="1" type="ORF">PGTUg99_017978</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="1 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1066946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000447">
        <property type="protein sequence ID" value="KAA1078801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1B8"/>
    <dbReference type="OrthoDB" id="2509247at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="89" mass="9673" checksum="8342DCA0242A2598" modified="2019-11-13" version="1">MPLEISCCAAQVGSLTKAPCRISNQPQYQPKAVVGSYPNRGHARSAHIGKLGQPWLRYLPRRDTPESLGGIGEVGSTLAPTSTPCWWNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1Z1</accession>
    <name>A0A5B0R1Z1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1Z1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="8404" checksum="A065565C444CF24D" modified="2019-11-13" version="1">MDDSTENTENTAVDVWPQEVFAQIFIRRQQLATSSHARQTMLLESATLLLDDDPNYDDLFGMHGGSKPGKKPNLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R5S3</accession>
    <name>A0A5B0R5S3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_037315</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R5S3"/>
    <dbReference type="OrthoDB" id="420187at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000248">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF758">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="186"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="106"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="508"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="547"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="591"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="621"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="659"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1120254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="696" mass="77270" checksum="779C2527C6AE25C9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RAT2</accession>
    <name>A0A5B0RAT2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036692</name>
        <name evidence="4" type="ORF">PGTUg99_035721</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000237">
        <property type="protein sequence ID" value="KAA1121934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RAT2"/>
    <dbReference type="OrthoDB" id="10284758at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474643" evidence="2">
        <location>
            <begin position="23"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1121934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="351" mass="38893" checksum="5EE9B3A42388D42A" modified="2019-11-13" version="1">MTINYSILLNHLFMFFISFTSGKPLWEPDNLMWSELGQVVEGGQIKPSLRQAPAQLTSGISVSDGLVTNRRSDSDFSASLSGAGSSRSSHPDQQLAITTPNFACNNQADLRRPLFPFRPLGPIELHRLEGGEWGHPDSSITINADSSLFPFAGSPTSTHLYQTNRAESKDESFVSSSHLLGSLPPSPSKGRKVGKRTNGRNPPVFSDLLVRAERSHDQSTSALTKSEIPNLWAPQPETAIMNTAFCNTIHDVYRSFTNQKGARVYNDKKHIKLYLGINKASKPESIVISNRSKTHSVYYTLHDPVAGLYVWERTLNPLCEESVVLHPMLGEGLLYAKGGEQKRVDPYRRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0REC2</accession>
    <name>A0A5B0REC2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_007432</name>
        <name evidence="7" type="ORF">PGTUg99_004582</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000211">
        <property type="protein sequence ID" value="KAA1123094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0REC2"/>
    <dbReference type="OrthoDB" id="9970295at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18822">
        <property type="entry name" value="GH43_CtGH43-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22925">
        <property type="entry name" value="GLYCOSYL HYDROLASE 43 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22925:SF3">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138138" evidence="5">
        <location>
            <begin position="21"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1071438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1123094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="577" mass="64359" checksum="6BF1EC93388FBE05" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RJT7</accession>
    <name>A0A5B0RJT7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034630</name>
        <name evidence="3" type="ORF">PGTUg99_019346</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000176">
        <property type="protein sequence ID" value="KAA1125669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RJT7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="96" mass="10930" checksum="8AAEDEB42C8FFBDD" modified="2019-11-13" version="1">MLIWKVSKLILCLAFLVGNVWNMLHHISRCVKSMTFPTKTEEAQCGHRGCEKKYFKNVEFICATCEARVTIHPNGCADHDPSSQTCQHNIPISRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RT99</accession>
    <name>A0A5B0RT99_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022341</name>
        <name evidence="3" type="ORF">PGTUg99_030495</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RT99"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="107" mass="11657" checksum="7A5446579C330483" modified="2019-11-13" version="1">MSWFSDKQKYPFLRWSNHAQTLGEEQNDGPKASTQQWKAQVLGMMDGRAVLLRLANGGRPTVVQPSPDHGPTKPPGSPSGDSWSLVGDSAWLERAWLQPSSDAAVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RVV6</accession>
    <name>A0A5B0RVV6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_005820</name>
        <name evidence="10" type="ORF">PGTUg99_011030</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000113">
        <property type="protein sequence ID" value="KAA1130101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RVV6"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017064">
        <property type="term" value="F:fatty acid amide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1300.10:FF:000003">
        <property type="entry name" value="Amidase signature enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052096">
        <property type="entry name" value="Endocannabinoid_amidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45847">
        <property type="entry name" value="FATTY ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45847:SF6">
        <property type="entry name" value="FATTY ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033845348" description="amidase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="82"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="233"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1108251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1130101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="618" mass="68902" checksum="90CC865B0D2A1420" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S6I4</accession>
    <name>A0A5B0S6I4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_028841</name>
        <name evidence="6" type="ORF">PGTUg99_023148</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRM CPSF6/7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1133025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S6I4"/>
    <dbReference type="OrthoDB" id="2507147at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034772">
        <property type="entry name" value="CPSF6/7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23204">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="207"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="309"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="484"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="111"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="501"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="515"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1084483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1133025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="526" mass="53127" checksum="A9B0CC8F8C8F7A3B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S835</accession>
    <name>A0A5B0S835_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013044</name>
        <name evidence="3" type="ORF">PGTUg99_015768</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S835"/>
    <dbReference type="OrthoDB" id="2508363at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="349" mass="40239" checksum="AE2A27E0A398C43F" modified="2019-11-13" version="1">MVRLSLNSSRLTIRLRYLWTPPPPELRTASHLRGLILKKEEFIRNYKPTDWELLSQEERLQIAIARIHREQDDTLAAQLLINETLREYKEYKREQKLRMSASTTTASTNPNTSAGAPNNHSAVDHIGNPEDTSVDELDELLNYPRDPTLPDRSQLRQDPGAPLPLEDRVRQLSMLQIKKKTAETTDSQITNASQNKTRDEAMDLDDPSTFSEDTPKRPNSPDIVALSKKERIRLLIKEHIAIWKKFELEKLSGATPELKTILHQAQDSQKALQKLTTKEELEGYVKGWNPWDAKRELFPPPPKKEGKKKSSTLRKAQQYDDPRVWADVFEIGQAWRAAYRQRSRKALPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SFW7</accession>
    <name>A0A5B0SFW7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001749</name>
        <name evidence="4" type="ORF">PGTUg99_005461</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000016">
        <property type="protein sequence ID" value="KAA1136898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SFW7"/>
    <dbReference type="OrthoDB" id="2506952at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1136898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="277" mass="30125" checksum="E892EA4FB5E317A5" modified="2019-11-13" version="1">MIRLLINRHHHQKQQHLAFEGLFSRPLITSQLNRPTKDVKASTKKYSTSSSKQEEVGGGGRIMLDEPIGIAEALRARYVRPLKTIYGRLGAKKSAADGTTARRKDADEEEGTAEGGGRSEEASQQQPLPESITEAWRLGLQGTHPARFSHLRARAATPLVVSSKPDRLPTSDLPSSADLLSPSSPSVEVPDPESPGAEAAKKKSLLQSYLALDKRSQKIISFSLFIWSLSGVIYFTWLSPFQSSSPTPPIISSARTRTNKILASSAKNLDDSKALSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LFV2</accession>
    <name>A0A5B0LFV2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_019291</name>
        <name evidence="2" type="ORF">PGTUg99_001397</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000537">
        <property type="protein sequence ID" value="KAA1063687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LFV2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1063687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="74" mass="7942" checksum="565B132F2B9A9A40" modified="2019-11-13" version="1">MSSTVTPSDSARHPSNACRSRYSSGRSWTGLSPPLPPLSAVNPSVHQCPDSPQQAFRNLAHSVYFSPLSRASES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LIY2</accession>
    <name>A0A5B0LIY2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LIY2"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="30"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="199" mass="22126" checksum="8DA7DC4FD090B5F3" modified="2019-11-13" version="1">MGGFTTVLYGLLYHSPTVTSRPILKGLPQPKLLGLIPLCPMLVIAPDSRPAYFVELFARAVCRFAGRMPLANANKGKNTPDTWFENQFASDPQCYVGNLRIATGLNILSALDFTNAHLAELVVPFQIMHGGCDRVTNPNGSISLFEHARATRKSIKIYPFVEHVMLRVGRDQSDDQDRQLIFTDIVEWIDGIIKATKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJU5</accession>
    <name>A0A5B0LJU5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glyoxal oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_009280</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJU5"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02851">
        <property type="entry name" value="E_set_GO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
        <property type="entry name" value="Galactose oxidase/kelch, beta-propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009880">
        <property type="entry name" value="Glyoxal_oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015202">
        <property type="entry name" value="GO-like_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208:SF96">
        <property type="entry name" value="GLYOXAL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07250">
        <property type="entry name" value="Glyoxal_oxid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09118">
        <property type="entry name" value="GO-like_E_set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glyoxal oxidase N-terminal" evidence="2">
        <location>
            <begin position="76"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose oxidase-like Early set" evidence="3">
        <location>
            <begin position="462"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1064605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="632" mass="67754" checksum="1734B6B9321F5C53" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKI4</accession>
    <name>A0A5B0LKI4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013787</name>
        <name evidence="3" type="ORF">PGTUg99_028030</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000506">
        <property type="protein sequence ID" value="KAA1068224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKI4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="194" mass="21591" checksum="75B91947809B724C" modified="2019-11-13" version="1">MVPPSSKATTLFPSDWFIERLYMSIASWSGISSARLMGRQVQLSTLRIFLVMKDLVMEDISYKLCSTHLTVGTDSVRTRLPFRDLKSCRHISRSDSGHGERMAKNLFYNVAAPGHTFHRVLFSPESVGKTEWSTSGTAGKSQNLTPNNSERELQPQKPLKPLMPDSSRLLSTTSTSTVTQILLPTTGQRQQSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKU9</accession>
    <name>A0A5B0LKU9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKU9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022755047" evidence="1">
        <location>
            <begin position="19"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="112" mass="12817" checksum="9123E4E058B8FA74" modified="2019-11-13" version="1">MRVLIVLLMTITAGSVLAELKPSEALKCGNCAQSKVMMELFPNENNLYPSSPYTGWHHCRLINNSNTNCGGLVQGKEYQCQRCKFSAFFPLDICKNCKTFHPVWYYKDTQTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLD1</accession>
    <name>A0A5B0LLD1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLD1"/>
    <dbReference type="OrthoDB" id="2508874at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="202" mass="22820" checksum="6D3265979A5A2B24" modified="2019-11-13" version="1">MTIPTESIPSPPDTFQESQVPIVFQETQGSEYIPNSQELESQDFPESLPVPDGSELYCKFDLDYSIWVNNSGNTSLVNNLRPAKPPCQLRMSVTDMSFSDFKTRVYNHLAATPDHSLLKSEHLVKALTNADKADQLQWCCSIKNCERVVTDDASFKSFVVVAGIQFDLSHEDLKELGFRRGPILLFRQGVDRLIHGKHEFDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPD0</accession>
    <name>A0A5B0LPD0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPD0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="14108" checksum="AC3C82F89B43F9C2" modified="2019-11-13" version="1">MLEGFPGESSFHLGFGFHPQSPCADSSRCATLNPVYHNLARLMPTWVQFAKNRDQGEKSNEVGWPIEKCVNEAVGDRMARKKTAVFNMLNLARSGGTVWKGGLKVGKLGKKRAGSVDLARQITSSKTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQ79</accession>
    <name>A0A5B0LQ79_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">dihydroxy-acid dehydratase</fullName>
            <ecNumber evidence="14">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGT21_027985</name>
        <name evidence="21" type="ORF">PGTUg99_017270</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
            <strain evidence="21 23">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14"/>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000142">
        <property type="protein sequence ID" value="KAA1128379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQ79"/>
    <dbReference type="OrthoDB" id="3851628at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050165">
        <property type="entry name" value="DHAD_IlvD/Edd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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            <begin position="1"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00006486"/>
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        <source>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022842"/>
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            <dbReference type="EMBL" id="KAA1128379.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000325313"/>
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    <sequence length="612" mass="64268" checksum="B1C3723EC2390138" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="3">Fungal N-terminal domain-containing protein</fullName>
        </recommendedName>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
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                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LV84</accession>
    <name>A0A5B0LV84_PUCGR</name>
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            <fullName evidence="3">Uncharacterized protein</fullName>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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            <end position="30"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1582" mass="172538" checksum="E2E52F1C93F867F9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWB5</accession>
    <name>A0A5B0LWB5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome P450 52A3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CYP52_1</name>
        <name evidence="7" type="ORF">PGT21_001299</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWB5"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="523"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1068772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="595" mass="66855" checksum="A8B70EB83349CA3F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXN0</accession>
    <name>A0A5B0LXN0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXN0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022683573" evidence="1">
        <location>
            <begin position="25"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="66" mass="7500" checksum="A7F6B5FB24FAB88E" modified="2019-11-13" version="1">MMKIVYHSLLYALFIQIFLHSVKAGCSSHPNAGWYVQRVILNDDGTHKAYVWECKKCRVTTTTPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYN2</accession>
    <name>A0A5B0LYN2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002809</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYN2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022676833" evidence="2">
        <location>
            <begin position="29"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="180" mass="20393" checksum="0A2B264ECA3BC19E" modified="2019-11-13" version="1">MFSPYVLSSLYLILLIFIEGSFSSPALGFDSISAKLNFRETPNFHGKPEGNDLQIHSSTSPHEDLSENNFNSDEESDYLSGYGSDESDDEYDSDHRSEDGEYHGGDKGYHDVKHEESLILESTGDTSSADNKSAHKTGRQNLSKKQQILFENLLQYYPGKEQLTTKDFLDIKENWKAHTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZS2</accession>
    <name>A0A5B0LZS2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZS2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="128" mass="14294" checksum="DAE2808EABFA9585" modified="2019-11-13" version="1">MEDTNENEISHEGLDAESIAILSAVEYPEDLYDFVPEASGSDVEEARETDQEYSGLDQHHLDSAFLRAMVIFHMNNTDIYPISIWGKNVVIAFLNETSPATDYYDQLPAHLLSPESRSDASGSRGRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0R2</accession>
    <name>A0A5B0M0R2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_050107</name>
        <name evidence="6" type="ORF">PGTUg99_050238</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0R2"/>
    <dbReference type="OrthoDB" id="2512852at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="398"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="472"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="417"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="472"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1070345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1090059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="540" mass="60286" checksum="8EB7A51EEAAB8CB8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2I2</accession>
    <name>A0A5B0M2I2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016099</name>
        <name evidence="3" type="ORF">PGTUg99_022559</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2I2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="118" mass="13304" checksum="71E779859FF7EFC4" modified="2019-11-13" version="1">MYLVNWKDFLPAGEVHVPQRLEGNPSSRPSTLPAPGILWDTRLPASFLRKRGHPHLLVGIRQGIEGIRNGYPLTISRDHRHTPISPPHLVRHSITSSSRPTHQQKYVRRGDGLNQTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3W1</accession>
    <name>A0A5B0M3W1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012818</name>
        <name evidence="2" type="ORF">PGTUg99_030669</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3W1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="101" mass="10506" checksum="AB5671328F46F4A9" modified="2019-11-13" version="1">MTNKSASGYADVCLRNGPACGRIIFKSGAVSGCFKFRLFTPGWRSGVCVCFDSAAGPLYAKDACTESPAPEDVSPAAIPAQSTPLVRPSETLKTCVSDKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6Z5</accession>
    <name>A0A5B0M6Z5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6Z5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="234" mass="26583" checksum="7DBE1F7D2CFF7A09" modified="2019-11-13" version="1">MKKETNDQHHVEIVITENYNIDHIRIEENHVAIEGLSHPWRPHSTGYPHTPSAEPNLILPTEHSWTWTCREARGEEEATVTEMNAKKKNLRCNLPRELTRTAFQPSAGSVCHPKPRSKAYKKHIVRASLVSEEKNIPLVHRFSTSTADHHHQATVNQRQGSGPGRGDIERFGDRIRSIHLASPLVMPSKICLARLRWVYVVPSMDLSSALQSSRDVFYCTLHQSILRATPSIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M752</accession>
    <name>A0A5B0M752_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033361</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M752"/>
    <dbReference type="OrthoDB" id="2505969at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="181" mass="20156" checksum="E5323C6CB15AC9A8" modified="2019-11-13" version="1">MSEAKSSSSCWTGVHAMYIVWISTWRSKVTLRTSHTLLESSDGKSKKPGKADARNPKAAARHLVAVNLLIASNFLFLFQATSEVQPVAFTLYICTERTLSISCDCNCTHYKLNPPQTLKPLRSRHCQKDDFGGRNTIGLQLNKGHLSAGRFPKTCISLHTLQAGKQLHRICPREKPDYPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8A8</accession>
    <name>A0A5B0M8A8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein fmp32, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_034379</name>
        <name evidence="12" type="ORF">PGTUg99_016323</name>
    </gene>
    <organism evidence="12 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="12 15">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCDC90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1072995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8A8"/>
    <dbReference type="OrthoDB" id="889336at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000018">
        <property type="entry name" value="Mitochondrial protein FMP32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF1">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="128"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1072995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1084698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="26452" checksum="149B1822E64F8B58" modified="2019-11-13" version="1">MMRPFGGSLGLKRPEILYGRTGAWWMRPGYPQRRRSQSISAPFSSCSSVQYAPYFDSHQFVSRLEKEGLSPVQAEGLMVAIEQVIDESMKNLITGLVTRSEMEKQQYTQRVDFAKLKSEIQLVEKQDFALLKSENERLISELEKLKQRLREEITRTQASVRLDLNLEKGRIRDELGVRELKIREVDTRIEGEIGNLRTTMESVKFNILQYVVGTVASGGALLLAYIRMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9Z6</accession>
    <name>A0A5B0M9Z6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034349</name>
        <name evidence="2" type="ORF">PGTUg99_016069</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1072993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9Z6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366129" evidence="1">
        <location>
            <begin position="28"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="166" mass="18465" checksum="5DBA2B8C4DA93308" modified="2019-11-13" version="1">MLLSSVNWITWISHIVLVMISLPSVRLDGPPPIGGGTVVPGPPRGTHSTATLRCLSFSTDTENLYVPKGKGFCTRPEGDWTCELPSCHFQNWPVLPLYFNKCKVLYSNAASYNLVVLVEQYVDYTPTAVHVSGFRVINNRASKTEEAFECLSPREQRPGCQDCVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ME96</accession>
    <name>A0A5B0ME96_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005835</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ME96"/>
    <dbReference type="OrthoDB" id="2496263at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022947994" evidence="2">
        <location>
            <begin position="26"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="263" mass="29383" checksum="31AAFE7597FDE8AD" modified="2019-11-13" version="1">MSVFSSSFAALLSLFIVVHLRLISSLPNPGNEHGSQTTLTNTTIEFGGPGVGAAPLFRKKPFENGPGPQIYPSGPTQPSPPALPRYFAWDRMLHIDKGNVVVVNVQTGEPVYTISQSPQDHRTTIVSNQHGDQLLSVNLKKTARRGHKFRYNTPSGVHYIIDPRGARTDRWYMVMEGKQAETFTYFRGHSKNAGNVYFQGQNKTKIASINFVKSLDKTHWKSDTLSGVKDIVTLEITQPTEIGDQYFIGLWALVKRRIDHYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEA6</accession>
    <name>A0A5B0MEA6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PZH1_1</name>
        <name evidence="10" type="ORF">PGT21_026242</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family. PP-Z subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEA6"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011159">
        <property type="entry name" value="PPPtase_PPZ/Ppq1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF484">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PP-Z1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000909">
        <property type="entry name" value="PPPtase_PPZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="257"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="56"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1074982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="455" mass="51769" checksum="342492EED762892D" modified="2019-11-13" version="1">MGNNPSKKLRTRLREREPSITEEPPADYEPTHSSSPQHQQQQPQQQQHQHHRKNNSISESTPQPTQSSSSKPLENNQQQQQQQQQQYQQQQTNNSNNDHQQQQLNNENNSTILANRPNPVLQTNGFTNTLSTTTTTTTNQAFDIDEIINRLLDVGYSTKITKSVCLKNSEITAISLAAREVLLSQPTLIELSPPVKIVGDVHGQYSDLIRLFEMCGFPPMANYLFLGDYVDRGKQSLETILLLLCYKIKYPENFFLLRGNHECANVTRVYGFYDECKRRCNIKIWKTFIDVFNCLPISAVVASKIFCVHGGLSPSLSTMDDIRRIQRPTDVPDFGLLNDLLWSDPSDTATDWEDNERGVSYCFGKSVIAEFLARYDFDLICRAHMVVEDGYEFWNERTLVTIFSAPNYCGEFDNFGAVMSVSEDLLCAFELLKPLDGAALKKEMQKKKRNSSVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEI3</accession>
    <name>A0A5B0MEI3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEI3"/>
    <dbReference type="OrthoDB" id="2498176at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38849">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38849:SF1">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022699462" evidence="1">
        <location>
            <begin position="23"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="202" mass="22106" checksum="51D9A40B8D3868EA" modified="2019-11-13" version="1">MQSILTIASVLIFFHSYLFIGAKPTGTLLPGSPSKTGPSKIFPYRKYEDFQISDGVAGQSLENAQKVFVTPFEGKLSEITQTDLNNLVAMSIEAIQNEQRFNQAQGKTGGSKKNGNQALAAGKTANKVLKLVGEIQVLELHKKLNLQQLADPEARLKEHQVKLAKNTKLDRANRGKKMVSFLSVGASGEKDIKEKKSHKGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MF26</accession>
    <name>A0A5B0MF26_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026627</name>
        <name evidence="1" type="ORF">PGTUg99_009212</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MF26"/>
    <dbReference type="OrthoDB" id="2513848at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="96" mass="10384" checksum="227305FE788DF000" modified="2019-11-13" version="1">MASSPGSGSTGQQKISHPILTVEPWDIHQLGANLLKDNQMYGSFMCIFFVVNLPLKGKANIGVELMGYGVESKAHPRRFLALLRSATRPTPVCCPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFL4</accession>
    <name>A0A5B0MFL4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 13">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="8 13">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RPE1_1</name>
        <name evidence="17" type="ORF">PGT21_021208</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="15">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate degradation; pentose phosphate pathway; D-xylulose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 13">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="8 13"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFL4"/>
    <dbReference type="OrthoDB" id="1927044at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00411"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000130">
        <property type="entry name" value="Ribulose-phosphate 3-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="12 15" id="KW-0170">Cobalt</keyword>
    <keyword evidence="11 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="10 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="153"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="204"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1074804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="235" mass="24694" checksum="4086FDF669D12843" modified="2019-11-13" version="1">MVKAIIAPSVLASDFGSLTAECKKVIKDGAEWLHMDVMDGHFVPNITIGAPVLASVAKTVEGAYMDCHMMVADPMRWIDDVAGAGGASYTFHLEACFQKPDSADHDPLAIIDRIHASGMRAGVAISPDTPSEKITDSIAAKADLLLVMTVHPGRGGQKFIDACVVKIAELRARFPQKDIQVDGGVGPKTVKPCAEAGSNVVVAGTAVFGAPQPGVVIQELKHAIETELRKAGTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MI47</accession>
    <name>A0A5B0MI47_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028132</name>
        <name evidence="2" type="ORF">PGTUg99_021045</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MI47"/>
    <dbReference type="OrthoDB" id="2510020at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="240" mass="27906" checksum="78C7A5449E3B9BBB" modified="2019-11-13" version="1">MSLFKGKKSIAEYERLVDESHELAESSNEKVSSSFPGFLRFSKNKNKKTASSSRSEPPEERDARSTAIVEDPIDSEEAITSHEKALAEKIENITNYVDKLYKKKYAFSWLQGTNLDEDLFKAFDARYIYGEPTLRALKKHFEAEMLYEQVPNKLSPKMESLIKNALQELSKLTKKDDQTGIKIAYYARVFAYMSGYHRNSRTPFNCLIMSHIKKNIQTSVKCLEKKYRPIFNQLNRIFER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNM1</accession>
    <name>A0A5B0MNM1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_021730</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNM1"/>
    <dbReference type="OrthoDB" id="2746at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000014">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.16, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="4">
        <location>
            <begin position="95"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1077838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="234" mass="25969" checksum="AF01A0EAD323586B" modified="2019-11-13" version="1">MPDNANPILKSSTPSHFSSLPSSSSVLTEHQERRKRFLGRVQEASGAQEDPYYLDSVDLLDDESEEDLKTGSLKLPDKRSGSDDEEQEEERIDSEEIFDLIRSITDPEHPLTLEDLSVVSPAQITVSYPPTDPDRSDSPHVLVRFTPTIPHCSMATLIGLTLRVRLLRSLPARFKVDIKIKEGSHQSENSVNKQLNDKERVAAALENNHLLGVVQQCLSTAHLRGAKPDGSSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MT54</accession>
    <name>A0A5B0MT54_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MT54"/>
    <dbReference type="OrthoDB" id="10290763at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="146" mass="15465" checksum="B2581564A56BA602" modified="2019-11-13" version="1">MQHSHDASQDGAHALAESNQTTNASWEGQQSSSAPPNSSANSTPVAMELEATEKVSKKIEHMTAQLDRILTNIKDVDTQLSRLAKSIEFGFGMGGNTAPLEGITAPLEGITTRLEGININEEALGISNQSANQVGQHANQVDQGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTL5</accession>
    <name>A0A5B0MTL5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTL5"/>
    <dbReference type="OrthoDB" id="2510196at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="486"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="534" mass="58861" checksum="650AC01F53A99CC6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWZ1</accession>
    <name>A0A5B0MWZ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024801</name>
        <name evidence="3" type="ORF">PGTUg99_011928</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000013">
        <property type="protein sequence ID" value="KAA1137042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWZ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137307" evidence="1">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="50" mass="5374" checksum="4062607774E575E1" modified="2019-11-13" version="1">MNFKSCLLAISVSLLIVLQSNGCGARPVVPVSEIEPIKTLGGGQLRNRMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZ61</accession>
    <name>A0A5B0MZ61_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009455</name>
        <name evidence="3" type="ORF">PGTUg99_024859</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000439">
        <property type="protein sequence ID" value="KAA1082167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZ61"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1082167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="57" mass="6088" checksum="F30378DA58DC4E8F" modified="2019-11-13" version="1">MNICPWPVVPATLNRLANRKSLHLNLDTGPQPILAPANQPLPQPPELPQPQTGSASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N017</accession>
    <name>A0A5B0N017_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007739</name>
        <name evidence="2" type="ORF">PGTUg99_020720</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N017"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1133514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="91" mass="10062" checksum="DAD7C095C2F8E14A" modified="2019-11-13" version="1">MMTNSPKSTYFSFPSYESHFIIEVEEAPAPATATLSQKALEPLIDCSAEAYPIIQRGSHLEAEIELILYLNAPRKPASLYSESSSSNSHQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5R7</accession>
    <name>A0A5B0N5R7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5R7"/>
    <dbReference type="OrthoDB" id="10395479at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="13664" checksum="E8995817AC4C2A5E" modified="2019-11-13" version="1">MQYQHVGGMLLGSACPNALGDGDNETEESKSEHNPGKDNGHDLQDINTDENKPLVKLNNTLWTHVTSAEKAYKDFVAKGDFMGSSLALEKIYAARYLHLLALAGNQNFLVSASSPPTDLKRTNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N811</accession>
    <name>A0A5B0N811_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N811"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="94" mass="10149" checksum="2E8732F4B9D8E3F1" modified="2019-11-13" version="1">MGSLGIDGSSSRGKSYFDGPLYEFKELFLLKGEEHSVTLDESDGVSESDKSTITNALLSQDKGSQASKIKASRNNKISASDLILKLAIFTAFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N828</accession>
    <name>A0A5B0N828_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphatidyl-N-methylethanolamine N-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">OPI3_3</name>
        <name evidence="7" type="synonym">OPI3_1</name>
        <name evidence="6" type="ORF">PGT21_008125</name>
        <name evidence="7" type="ORF">PGTUg99_009896</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000213">
        <property type="protein sequence ID" value="KAA1122968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N828"/>
    <dbReference type="OrthoDB" id="8300106at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024960">
        <property type="entry name" value="PEMT/MFAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458:SF5">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1085467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1122968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="75" mass="8945" checksum="CDEDA693F2BCDFCB" modified="2019-11-13" version="1">MSPIELGWYEGGLIDLRRHSFWLSLAWILFNRIFWNFIARQEHLTRFLTRLLGSPHRGCYFLGGVIFIIGLLRDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NB81</accession>
    <name>A0A5B0NB81_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Delta 8-(E)-sphingolipid desaturase</fullName>
            <ecNumber evidence="5">1.14.19.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGT21_025802</name>
        <name evidence="19" type="ORF">PGTUg99_023310</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
            <strain evidence="19 21">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.18" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NB81"/>
    <dbReference type="OrthoDB" id="260091at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF30">
        <property type="entry name" value="DELTA 8-(E)-SPHINGOLIPID DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015921">
        <property type="entry name" value="FA_sphinglp_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="17">
        <location>
            <begin position="20"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1085962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1113808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="591" mass="67690" checksum="E0C2A198327078BE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEK2</accession>
    <name>A0A5B0NEK2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036392</name>
        <name evidence="3" type="ORF">PGTUg99_008996</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000003">
        <property type="protein sequence ID" value="KAA1138153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEK2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137457" evidence="1">
        <location>
            <begin position="24"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1138153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="118" mass="13401" checksum="F66764D9F080B1B8" modified="2019-11-13" version="1">MIVFPCYLHVVVLLATCCLQTISHMDLPLEGVHQTESNGIMNIDDEEMNRILMVNKLKLKDVPNAEEQGKILEFIEAIPQLRSRPNSGLVRDYVMNPDTEEGKKCTELTVITIMMIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFJ0</accession>
    <name>A0A5B0NFJ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ccr4 associated factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CAF17_2</name>
        <name evidence="5" type="ORF">PGT21_033677</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GcvT family. CAF17/IBA57 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFJ0"/>
    <dbReference type="OrthoDB" id="191995at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000050">
        <property type="entry name" value="Putative transferase caf17 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GCV_T_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602:SF0">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00093447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1087563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="403" mass="45268" checksum="BF8BD6F2066206F1" modified="2019-11-13" version="1">MRPVILTTPRSVWVGLSGHSHSFHSSSRATEWLRTRLVDRGLISLKGEKSKTFLQGLITNNLNRLSSQEEAHHNTAFYTAFLTPPGRLQFDGFVYPEPPENEAQCLLIDHHHPEADRLLAWLRRFVLNSRVKISKDSRELWAVWPNHPLDNIQSLLPNSSLLEQPSNHAWKDHRGDQRLGYRIIGNPESVPELEPLAQLPEAPLSAYALHVLLHASLPPSLSPPYPVTLPFEANLDYHHGVDFRKGCYVGQELTARTYHTGVIRKRMVPVSIEKDGLPALASVPDRLSISDTQMADRPLTERLEGHELRLGSQPPNTSTIPAQKQKTIGKLCGPFISTSPVDHHSSDGPRSLLYGLALLRIDHDAFSPSVEHPWPTISLSTVDQDLSPWTIQPIKPVWWPSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGC2</accession>
    <name>A0A5B0NGC2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGC2"/>
    <dbReference type="OrthoDB" id="2507615at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022933667" evidence="1">
        <location>
            <begin position="20"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="560" mass="63660" checksum="B4B7B53D16D9D07B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI33</accession>
    <name>A0A5B0NI33_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI33"/>
    <dbReference type="OrthoDB" id="2505774at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1088985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="451" mass="51026" checksum="CA98CF1F276F9E69" modified="2019-11-13" version="1">MVRDLMGGLKPTQLDQIEGRYRAFNLVSLNIPWLLPHYMAKHSSNFVGKEFKAVLQSAPFVLFEFIDDPERLAWVALCQLAPLVFQTHIDDMESYLTLLRFHIEKFLYYIFKITAQWVNKPKFHMLLHLLDSIERFGPASLYATEKFKSYNGTLRKAAVHSNRQSPGKDIAKSFASYKCIRHLTCGGWFKDPKDSTRYITAAPSVGNMFLERPSLQKTMDYNHLAHIGREGVFPRVINTKVTAPDTIPAGLQKHLPGRRILQLGAIQWTPHRVLKKGVFVLVRLGNSAEGTALTVGCIDHVWEATFRGRVSFWVCYTEYGRGGVDSYYQMRSVKKSTTHKYVHIKGIESTINVQHNCHAGGCQVSPTGRVRIEREDSEENNNIVVHGDTDEYVVNSGELNSTQVLRTWVDVPRGGEEIGDLIPALNKGLATWLQAAGESDDDEVLDNPSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NII8</accession>
    <name>A0A5B0NII8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007962</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NII8"/>
    <dbReference type="OrthoDB" id="37537at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF78">
        <property type="entry name" value="PYRIDOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="209" mass="23466" checksum="7E9B3139C9B0B483" modified="2019-11-13" version="1">MARVDQKVPIEDVMKSYKTLINEGKFKHVGLSEVSAETIRRAHAVYPVSAVEVEYSPWLLDIEHNGILSTCEELRIPIIAYSPLGLGMLTGKIKSLDDLDPNDARRHFDRFQPENFHHNIKLTDKFISLAQKKQCTPVQLGLAWILMQSELLIPIPGSTRAEGVEESLASLKVKLSDDEVKEIRDFVDKADFKGVRYSASHKAALNLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NR73</accession>
    <name>A0A5B0NR73_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024070</name>
        <name evidence="2" type="ORF">PGTUg99_003627</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000220">
        <property type="protein sequence ID" value="KAA1122688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NR73"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1122688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="114" mass="12790" checksum="76B111454EF02073" modified="2019-11-13" version="1">MKGWDLNQEPEAELKRIVLLDSEFAPNESRSGSGVLSGRRLEDQRWGLCTTCLQDRGHDTRPPRVSVVPRFPTPMGDQAFSASQLRAAPLEAWSHHLHILKDPTDASLSKYLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NS87</accession>
    <name>A0A5B0NS87_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_011034</name>
        <name evidence="2" type="ORF">PGTUg99_015159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NS87"/>
    <dbReference type="OrthoDB" id="2498190at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="1">
        <location>
            <begin position="7"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="112" mass="12176" checksum="D0D16FFDC6956487" modified="2019-11-13" version="1">MSSQQAPLFIKIKHANSTRKVRVPNQPAPVWSKLSAAIVDRFGIPEGQPIGLQYLDPEGDIITISSQVEFDELKGLPRWPGFRGCPGPEPRLSGLGMGICQTPKPRGFLGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSJ7</accession>
    <name>A0A5B0NSJ7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050250</name>
        <name evidence="3" type="ORF">PGTUg99_050134</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000354">
        <property type="protein sequence ID" value="KAA1097097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NSJ7"/>
    <dbReference type="OrthoDB" id="2505980at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="151"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="341" mass="39127" checksum="9F43628C30FF5F11" modified="2019-11-13" version="1">MPYRFIEPGIKIAVVRMVTQSYSQRFICETLGEPISKQFFARWMKLYQNTQRVIRDPAEYNKRGPGLLLTPDDRVFMIELLRLEPGLFLDEMRERLYDQNDSLLSITTLHRMLVEHLEVTLKKANTVSIKKSLVAKYAYVVQMMTVPADFLVFTDESSVCSKQLLRTFSRSTKGTAADRVLVDQNAKRFTLIPAICLDGVVALTVTDENVKGRNFAHFLKFILLPRMNAYPARNSILVLDNAQIHANERIAKLCADVGVRLVYLPPYCPELNPIELVFLQVKSNLRRLQVLVTSQDPVWAIESTTYRVASPRLCQKLFHHAGYDCPESSGDQDMSEFYFCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSS8</accession>
    <name>A0A5B0NSS8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024318</name>
        <name evidence="3" type="ORF">PGTUg99_015421</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NSS8"/>
    <dbReference type="OrthoDB" id="26149at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040144">
        <property type="entry name" value="RAP1GDS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10957">
        <property type="entry name" value="RAP1 GTPASE-GDP DISSOCIATION STIMULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="655"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="751"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="796" mass="89086" checksum="04DB2F763B5FB63A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NU04</accession>
    <name>A0A5B0NU04_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Bud site selection protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BUD20_1</name>
        <name evidence="13" type="synonym">BUD20_2</name>
        <name evidence="13" type="ORF">PGT21_034749</name>
        <name evidence="14" type="ORF">PGTUg99_010140</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ZNF593/BUD20 C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000379">
        <property type="protein sequence ID" value="KAA1091994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NU04"/>
    <dbReference type="OrthoDB" id="24683at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000299">
        <property type="entry name" value="Zinc finger protein 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051879">
        <property type="entry name" value="C2H2-ZF_Maturation_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1081369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1091994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="146" mass="16326" checksum="E1DDC766ABDADE1B" modified="2019-11-13" version="1">MGRLRRSRTHSSAKSQIKSRKTKRYTRDLDQIAIDMELAQSIRSVKQAQSDSQSNFVVPQAFGSTSHPTVTGEVDEDTTGCGQFPCVQCARFFVDSRSLTEHQRGKVHKRRVKELAAGPYSLEEAMRAAGIGTDNRQRRAPDLMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NV08</accession>
    <name>A0A5B0NV08_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022418</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NV08"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1093041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="119" mass="13420" checksum="39D94EC41A75ACAB" modified="2019-11-13" version="1">MTNLTQKQCVNLVDGVKLGGLSRCVLCGTLQRRGQASRIGGKKHLCKKKKRGEKDVFITVAGMTLHMLIGEVTHVTLKKAVYQYQYCLWYSYQISNIQGVKCILLPRLEFMNEKTNPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NV37</accession>
    <name>A0A5B0NV37_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009476</name>
        <name evidence="2" type="ORF">PGTUg99_025424</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NV37"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1092642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="71" mass="8031" checksum="758989CCE70CC426" modified="2019-11-13" version="1">MPSNKDYYLRIEAIYSPTTSLNSFHSHSSSSLPVSTSSSTFRRNLISVHQVWRPTHPHRLAKHLSGPLVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZK4</accession>
    <name>A0A5B0NZK4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ZW10 C-terminal helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027801</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZK4"/>
    <dbReference type="OrthoDB" id="534815at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055148">
        <property type="entry name" value="ZW10_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205">
        <property type="entry name" value="CENTROMERE/KINETOCHORE PROTEIN ZW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205:SF0">
        <property type="entry name" value="CENTROMERE_KINETOCHORE PROTEIN ZW10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22766">
        <property type="entry name" value="ZW10_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZW10 C-terminal helical" evidence="2">
        <location>
            <begin position="918"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="542"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="564"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="679"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="733"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1066" mass="118764" checksum="FE352912EAC1F279" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1E1</accession>
    <name>A0A5B0P1E1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1E1"/>
    <dbReference type="OrthoDB" id="2511077at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="445"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="354"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="468"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="482" mass="55984" checksum="7DB52D6CB66F6D82" modified="2019-11-13" version="1">MDSGRIPTQPHMASQTNGTNPHELGREGLSDREKIKLYELEIEKLQASLGTSNYKLKKQSLLIRALHIQALGKAIPRLSAVRPVHKWQDRRNRDELGGADFRVERALLPRLRRRILEMTSVLEPSRFEEGIFSEHLETEVGWLDQIDADSCQVKSSVLIPRSEWKSYHLRGVPETVRHQELLRFKQGGRSPYILERYYYPEISWGADLIRDGLIRLYESHIETLQAAEPQSVSLKGTSLGDLLGLIDQMIWNLDHPRKVLQAKWQRVVREIEIMELAIIQFQKPEESRTDLNVEGIDHNIHLKSQYWIPRDEVEDFEIAFLQSYLPLLRVCRVLVNKLSSSASSTPLMAFYDDLNKLNRLYSDTEEMEDQLRKLTLLVLQLRSRRARIRSKDHLGLFRWLHSKIQTHLNSLNCGTDQTQIQKAREWCLMWEVQYNTALANFLDGELSESDGEEDDGSEATDSPFYGDDSTDEEEYDDDEDGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2P6</accession>
    <name>A0A5B0P2P6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2P6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="116" mass="12564" checksum="94CEC315567DAB88" modified="2019-11-13" version="1">MIPQPRPHSFAAISSAAITPTSHPSERTYPPGTNRPKAYTRPRRAKFRGILVQPDQSGSLQSEPIASSLLVVVTLRLHPRASQKDRDGPVASFLTGSFCLIKSLHNIPAPSVSITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P399</accession>
    <name>A0A5B0P399_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mss4p nuclear export</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">BCP1_1</name>
        <name evidence="3" type="ORF">PGT21_024174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P399"/>
    <dbReference type="OrthoDB" id="27543at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025602">
        <property type="entry name" value="BCP1_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261">
        <property type="entry name" value="BRCA2 AND CDKN1A INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261:SF0">
        <property type="entry name" value="BRCA2 AND CDKN1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13862">
        <property type="entry name" value="BCCIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="312" mass="34909" checksum="53A09935F3E565CB" modified="2019-11-13" version="1">MAQSNNIKKRMFEGEEPVRDQAGAENKDQEKIEVTFEFCDLNPIDYHAIKHLISQLFRIARPKSSTNLAPTSNPTPPSKAALKPVLEDLDVGELADLVLSDQKEWVGCTVKCDEEDPDPYAFISVLDLKAYQDKKSILTLTNYLTSALASSNPSPAQADELLKVLKESLQPEGSGIGLVLSERLINVPVYIMPQLFTQLGNEIKHAQSKNAPGFNFDRLLIPSRVFTLSPNQDSSQDVNPTFDSNTSTNNNVQKPKKNKTDANLNPDDPDQVGLYHPEDQIISQVELFIVPLISLERKALKKRDRRSYTLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3Z9</accession>
    <name>A0A5B0P3Z9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_050031</name>
        <name evidence="14" type="ORF">PGTUg99_050084</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000315">
        <property type="protein sequence ID" value="KAA1104923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3Z9"/>
    <dbReference type="OrthoDB" id="308440at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025525">
        <property type="entry name" value="hAT-like_transposase_RNase-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14372">
        <property type="entry name" value="hAT-like_RNase-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140996">
        <property type="entry name" value="Hermes dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="12">
        <location>
            <begin position="44"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1096171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1104923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="662" mass="75452" checksum="F6FCDE907C7D9B0C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5E6</accession>
    <name>A0A5B0P5E6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">5'-3' exoribonuclease</fullName>
            <ecNumber evidence="7">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_025728</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Possesses 5'-&gt;3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5E6"/>
    <dbReference type="OrthoDB" id="372487at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09897">
        <property type="entry name" value="H3TH_FEN1-XPG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017151">
        <property type="entry name" value="Xrn2/3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF7">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037239">
        <property type="entry name" value="Exonuclease_Xrn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="9">
        <location>
            <begin position="473"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1096705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="715" mass="82888" checksum="B37FA1E17D220566" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7M5</accession>
    <name>A0A5B0P7M5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7M5"/>
    <dbReference type="OrthoDB" id="5535068at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="179" mass="19826" checksum="F9259D0FC8E6F6B3" modified="2019-11-13" version="1">MEEDPSKVTLSSAQTTEEINCQVFNALPSPGSIGVKLNGYDCEGLLCPGLLFNFISEEEAQKRDFDVHPQDIWMPGFCDDDINITGIALASLSMGGICETTSFLITQRPGPLVLGKPFMKDFSAVIDYKEPEPPTLTVLDCEGIKHTFQISQVKQERAHPPGINKQNTSSFYLYQFPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8X0</accession>
    <name>A0A5B0P8X0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026964</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8X0"/>
    <dbReference type="OrthoDB" id="2246127at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="368" mass="41503" checksum="EAE0B1FB30D524E9" modified="2019-11-13" version="1">MSIFKTPTYRLSLANIIAQEVANPFVSPVIEYFPQEADGRGVYKLSQSKKWLEGFEPTLRAPMCRNNGRDWFLFEPVQLNTDEIVVPVFFFMRGTVMFAKYNLETVKVSDFEYDCLEVIGTNGRLLSTQCRNSIFGLGNQVTTWHHLPNPWRKKANGWNKHISYYFTLSGLPPTWTNQNYNCHYLSTSNEAGAMELAGPIVSDLKAMAAEGYPAYDCTINEEVLVVSTILCFLGDSPMHAEITSTPIPGNSLHPCRACPLSSVSVKAKATLDYVKRFLMIGPSGSWISNPTRSWQKIKTRCYETWVMAQKPRTKTKNQFSDLVRPACLQLKNLKAITVFCEMRRSTQTGTVRVKTLATLLLITATCVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PE43</accession>
    <name>A0A5B0PE43_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PE43"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="258" mass="28740" checksum="3D441100F4505F9C" modified="2019-11-13" version="1">MPPGRPPMLPTDASPAPDPSAHCNLHGINTTDVPCVPPWANTAMEFSETSSLARLPQITRLDNEIYHDCAIHLGFQSLSSSTGGYPSAQPASFQCQGVNKGKKRESVLEESSNHSPKRALNNHQSNSPMASTSQGRVNTVEMEQMTLGELFVERQLAEAQFDECKRNPADAPSRREDFVAGRPTIPESTLLKEKFVMAETLEPMKEVHDLQFQCPNSEMLSFLKRHYSDANKAVQSLTLIDEVSWHHDRIYVPESLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PF34</accession>
    <name>A0A5B0PF34_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_006756</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PF34"/>
    <dbReference type="OrthoDB" id="3364440at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF2">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS catalytic" evidence="5">
        <location>
            <begin position="126"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1099412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="297" mass="32819" checksum="D4D8E614E19F6813" modified="2019-11-13" version="1">MPHASEAATDAVQPKFVRLCWIDLTGRVRCRIVHRTRYEQMIQSPPESILSLPACVMGLGHQDELAPGFGPSGDLYLQPDKISYRVLTYFPTHAMVMCSLLRRREPDSHQASENHSSHFSPYPLCPRFILSSTLHSGIAESGVSYLIGFEIEVVLLKSLQPLEAVDSGPHFWSGASSLRNGAAGLACIEKVVGYLEEANITVEQWHAASAPGQFELVLSPHAPFQSCDELIYAKELIYSVAHEMGLKATFSPKPFQAARGTRSPMHISLHKKNKCCLDRRSARDTNADRASSSRLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PK15</accession>
    <name>A0A5B0PK15_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032631</name>
        <name evidence="2" type="ORF">PGTUg99_001077</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PK15"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1100249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="98" mass="10593" checksum="E138A65B04F8550D" modified="2019-11-13" version="1">MALHSSTTTHPDKLNDKALAASRVRYSPDALPFVSRLETTLTAQVTQTAGWSSRDNPRASIHTPLHSTCRLVVARQPSGLNPYSPPFNVSITGPILEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PPU5</accession>
    <name>A0A5B0PPU5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034715</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PPU5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="11152" checksum="C5FDB60F0BDFB19F" modified="2019-11-13" version="1">MPTDDLTGTLNCTDCLDYMHTKIQTVHDGLTAKRDRDSPTDVPHELIITKPPRARFHESQIPARNRNRSGSKDKLLQIAALRVKAQLEMLFPTSGPSAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PR74</accession>
    <name>A0A5B0PR74_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Adenine DNA glycosylase</fullName>
            <ecNumber evidence="3 13">3.2.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_010045</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Adenine glycosylase active on G-A mispairs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.</text>
            <dbReference type="EC" id="3.2.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.31" evidence="3 13"/>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PR74"/>
    <dbReference type="OrthoDB" id="10248838at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035485">
        <property type="term" value="F:adenine/guanine mispair binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032357">
        <property type="term" value="F:oxidized purine DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000701">
        <property type="term" value="F:purine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03431">
        <property type="entry name" value="NUDIX_DNA_Glycosylase_C-MutY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000002">
        <property type="entry name" value="Adenine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000002">
        <property type="entry name" value="Adenine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044298">
        <property type="entry name" value="MIG/MutY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029119">
        <property type="entry name" value="MutY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944">
        <property type="entry name" value="ADENINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944:SF1">
        <property type="entry name" value="ADENINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14815">
        <property type="entry name" value="NUDIX_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="15">
        <location>
            <begin position="164"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="590"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1103213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="614" mass="67960" checksum="842706C89171B1AE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSJ3</accession>
    <name>A0A5B0PSJ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">XPG N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_035440</name>
        <name evidence="8" type="ORF">PGT21_035524</name>
        <name evidence="10" type="ORF">PGTUg99_001419</name>
        <name evidence="9" type="ORF">PGTUg99_002791</name>
        <name evidence="7" type="ORF">PGTUg99_010020</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPG/RAD2 endonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000329">
        <property type="protein sequence ID" value="KAA1103600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000070">
        <property type="protein sequence ID" value="KAA1133719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSJ3"/>
    <dbReference type="OMA" id="RFYQTKV"/>
    <dbReference type="OrthoDB" id="17262at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09902">
        <property type="entry name" value="H3TH_MKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09858">
        <property type="entry name" value="PIN_MKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000041">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.259, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022039">
        <property type="entry name" value="MKT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037314">
        <property type="entry name" value="MKT1_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022040">
        <property type="entry name" value="MKT1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF32">
        <property type="entry name" value="POST-TRANSCRIPTIONAL REGULATOR MKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12246">
        <property type="entry name" value="MKT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12247">
        <property type="entry name" value="MKT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Post-transcriptional regulator MKT1 N-terminal" evidence="5">
        <location>
            <begin position="332"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Post-transcriptional regulator MKT1 C-terminal" evidence="4">
        <location>
            <begin position="514"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1098455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1100293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1102053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1103600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1133719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="767" mass="87361" checksum="CD37F1D592CD980E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUX5</accession>
    <name>A0A5B0PUX5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031567</name>
        <name evidence="1" type="ORF">PGTUg99_006940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000344">
        <property type="protein sequence ID" value="KAA1098958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUX5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="106" mass="11794" checksum="DE4BA09A3E2541D9" modified="2019-11-13" version="1">MHGPWYLVTRQTQVQFQIVGQLLCPQWKHVALSNCPSLIQDSIRQKISTPNGQRHPSDKIAPTRRVINPASNHPILATFRLLSQSSNHLIVVNNPPLSTPADSATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVB8</accession>
    <name>A0A5B0PVB8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVB8"/>
    <dbReference type="OrthoDB" id="3264316at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="762"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="703"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="703"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="850" mass="95100" checksum="0D4CB9B7B92C15C0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PY02</accession>
    <name>A0A5B0PY02_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020221</name>
        <name evidence="4" type="ORF">PGTUg99_013190</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000041">
        <property type="protein sequence ID" value="KAA1135108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PY02"/>
    <dbReference type="OrthoDB" id="2501437at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF3">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="45"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1135108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="325" mass="36741" checksum="75D7B48D45F37EDA" modified="2019-11-13" version="1">MQTRIEEVVGGIEEVVQMIKPVVTPGPSRAIVDSPGPPMSKPICRWTEEDDLTLIAFLKAELQRNPSPANGYKGSTWPAAARALAGSEEKTGSKAKDATGCRSRYSSLKRSYLELKHLRSLDGARWDEKMQRVILPEDIWTSFLANSSEKGKSLYRWRQKHFPLYHQVSDLIEGNGVAINEPKETADRGAARWVSRGSSPDQQDESTPNESFDPLLWQSDRLEEEDQEDQHRDQPILSAPPSAKRRRITQQDLFLKHLKDIGDNLKIRPSVRVDAIRLLQLDGQLEGPELLKAIDYLSPLEHAETYIALDARLRTTWIRSKLGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYJ8</accession>
    <name>A0A5B0PYJ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021684</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYJ8"/>
    <dbReference type="OrthoDB" id="2508582at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022836244" evidence="2">
        <location>
            <begin position="21"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="133" mass="14455" checksum="3AB32AF99E471644" modified="2019-11-13" version="1">MPSIISIMFFAILACGFATADLATDQKMTCAFHCPTLTEGGCVRNKGRDKKGNVNKWEVVKAFATLGYAEYYNCLGLNADLSVCAEMGKIVLPPSPQPQVMTINSEKTRKELGEDSKPFDITAGDPEECSYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0D6</accession>
    <name>A0A5B0Q0D6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034979</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0D6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1106444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="119" mass="13287" checksum="23812A1D40CA201F" modified="2019-11-13" version="1">MGCWIERCLLQLLGDFQPWDAVRQGPSSESDETAGAHLRHEGHVLKQQSESHVKLSIASKSQDESYAIAFPRSRNPSLKSRSKNTVFALLQESLQSTVTLSTPLSFLYIWESSVPIDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q451</accession>
    <name>A0A5B0Q451_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029186</name>
        <name evidence="2" type="ORF">PGTUg99_026993</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1108055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q451"/>
    <dbReference type="OrthoDB" id="10403183at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="77" mass="8445" checksum="A8252ED28BEC996E" modified="2019-11-13" version="1">MKWKSGWSLSTGVRKGLTSYVLAPLTPALTSAVRPPKEALATKPQADMPGAAYDYNLETSIFSNKRENYQKPNSPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4K1</accession>
    <name>A0A5B0Q4K1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003836</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4K1"/>
    <dbReference type="OrthoDB" id="1301613at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272">
        <property type="entry name" value="BED FINGER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23272:SF161">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN RICESLEEPER 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="2"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="70" mass="7754" checksum="1B4FB74E18489F94" modified="2019-11-13" version="1">MVLMIPATSIALESAFSTSGRVLGDFQSRMAANTLKALVCGQDWFRSEEDVNNNKDDEEEEGDDDIELVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5S3</accession>
    <name>A0A5B0Q5S3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5S3"/>
    <dbReference type="OrthoDB" id="2508573at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="278" mass="31836" checksum="95E522D01C11F345" modified="2019-11-13" version="1">MKDWCYKFIAYILIKAAPSNGFIYKWTLTTDVSSSLIFGNVHLGNARFDHDGLITVDMRVGTGRFSLQENENEENLHSLPYTTLNKPYQLYTPVGNELEFSRTVLYLHNLDLMTCGWTIYHDVERTSLQDHRDIELAAARRQRVGTEQHIQQTISVEPPSSEAIRTRRRTSRLSLGRSGVPIVPVNDRKIPENLADQEIVVWNYRFSEDYAAIIADHNKQVLRDLDHSNGDNSDSDEDKDLNRGRSFLEPGNKDQAGFRIDLERTGPVGPFFETFKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q708</accession>
    <name>A0A5B0Q708_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_029068</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q708"/>
    <dbReference type="OrthoDB" id="2497484at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02181">
        <property type="entry name" value="GH16_fungal_Lam16A_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000114">
        <property type="entry name" value="Probable endo-1,3(4)-beta-glucanase NFIA_089530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF24">
        <property type="entry name" value="GLYCOSIDASE C21B10.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26113">
        <property type="entry name" value="GH16_XgeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022721428" description="GH16 domain-containing protein" evidence="5">
        <location>
            <begin position="27"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="6">
        <location>
            <begin position="112"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1108921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="426" mass="46766" checksum="6F65202DA46BD771" modified="2019-11-13" version="1">MSRTKVLSTAFLIGCFLLLDLSYTSAECAPGSSPHHHRPHRGGKHPKLRDQVDNGEYGSKPSYIRKQDDDGDYGSNGDYASHLRAQDDNGDYGSGSYNPNSNLLGLDLPIQLGYGREPNSWGKKYNLTLSSSGNKFFDDWDFFSDSDPTHGQVAYQPVNEAWKQGLVSVGPSSHDKNLITAKMKVDNTNWLAQGEARKSVRLSSKKSFKYGLLVLDALKMPFGCSTWPAFWTVGNNWPHDGEIDIVEGVNMLNTNQMTLHTGPGCTIQNPMLQAAVGNVLNPDCDVYASSNLGCGVHDHSNASYGQPFNQAGGGVFAMEWSPNGVSIWRFSRGEVPRDLQSGHAPQPSTWPIRPVAHWSSDICNNMNDEFSEHRIIFDITLCGDWAGSAGVFNANNACSGSCTDLVKDPTNYKDANWEIASVKLYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q729</accession>
    <name>A0A5B0Q729_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q729"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023031031" evidence="1">
        <location>
            <begin position="20"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="228" mass="25923" checksum="E2D1FC3ACC5863ED" modified="2019-11-13" version="1">MMTYARICWLMYRWMVAPGCSLWRKSCRDSPDLLASRSTFPNRPHVGSLEHELHQSPPDNPLWLTEVPFIHSTPGQPVFFNTSNIYSSKDRVYILGLLSNDESIEKKNTTLFLRTTNQPAVFDPTKHPNQTKTSFRSILIPQLLSPPATWLGDPKAFQAHLPRFSSCHRHSPSALVSLPAAVPIFRHRSRLFLHMYADNSSSISTCKNTIPARNNRGPHIIHTANERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBF7</accession>
    <name>A0A5B0QBF7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBF7"/>
    <dbReference type="OrthoDB" id="2508713at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="152" mass="17781" checksum="2C6283310CA3B28F" modified="2019-11-13" version="1">MEVAEQLTLDTSDLGDAVEQRIQRRGMSSTLFLKMKEDIEHPRLVNVAPLLGQPPQKPPIDVAECAYLLIHVARMDLQARVASSVLRRERERADLNINHAQVRIRLNELLQHISQEDKKLRDAHYDEFQRENQQEEEYVDSYFTLFYNSTPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBH9</accession>
    <name>A0A5B0QBH9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBH9"/>
    <dbReference type="OrthoDB" id="2511857at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022694992" evidence="1">
        <location>
            <begin position="22"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="13661" checksum="2D825DC7F5595946" modified="2019-11-13" version="1">MQSVLVAASLLCLSLLQGGLAAPTPLPTDQEFSKQAEPHVSLDKRQLMSYPGFANNLYNPYFGNRFYSGSSSGFNSLSYSDSVSTYSPWGSYNYLSRGNQMNSYSNNFSGLYAKDADHTKPTNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC75</accession>
    <name>A0A5B0QC75_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029483</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QC75"/>
    <dbReference type="OrthoDB" id="10274438at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="135" mass="15167" checksum="180692B482EEE194" modified="2019-11-13" version="1">MEHPEFMNSYLTNHVTLNLLRREYQAEAASLNAVPNEFHDPTNLSLPLLEAIQNTVEEMKGLKGGNDRDIAKIALYARALAEITPNSPITSLTPLHYTVKALVRHRWAELICGMDYRSATILEGLNKLWDVPGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QH65</accession>
    <name>A0A5B0QH65_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cullin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_001975</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QH65"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000007">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000011">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000026">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000029">
        <property type="entry name" value="Cullin homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1030.10:FF:000002">
        <property type="entry name" value="cullin homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1030.10">
        <property type="entry name" value="Ring Box Chain A, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="9">
        <location>
            <begin position="420"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1112556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="771" mass="88524" checksum="A7BEDF5E57E81DF9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJY5</accession>
    <name>A0A5B0QJY5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032771</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJY5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="85" mass="9543" checksum="88494EF985CA1891" modified="2019-11-13" version="1">MGNNQICSQTAEESIFFPCYRWRPMCNPNMTFPTRSLSLQAYQGSESIIRHDGPTFDSSQLELTASPSGLLTPLWDVTDEICDPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QK30</accession>
    <name>A0A5B0QK30_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptide hydrolase</fullName>
            <ecNumber evidence="7">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_032524</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QK30"/>
    <dbReference type="OrthoDB" id="10013407at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF26">
        <property type="entry name" value="PEPTIDASE M28 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M28" evidence="9">
        <location>
            <begin position="354"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="128"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1113490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="589" mass="65784" checksum="E6B5EC8FCB320F36" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QK43</accession>
    <name>A0A5B0QK43_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033275</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QK43"/>
    <dbReference type="OrthoDB" id="2501436at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7742" checksum="1244932A6ABA24C0" modified="2019-11-13" version="1">MQYGFGPASQVTREGSSTCTVSRLPSKSIIDVCCENPVGLRNPRPFESVFTVSENDYKNTFKCREPTDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QLY7</accession>
    <name>A0A5B0QLY7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QLY7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="64" mass="7052" checksum="7351E63E9ADD8C3C" modified="2019-11-13" version="1">MPSRSLEEGSLRLYTPTANLLAYVMATWPSGLRRRLKEVAGTSTSILAVIRRSSGIEENTKDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN22</accession>
    <name>A0A5B0QN22_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN22"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023058189" description="Secreted protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15180" checksum="84A4FDE7F9075F74" modified="2019-11-13" version="1">MNFKTIILGVIMAFTLISQVSAIKCILEGCGGEARQCRSRPVNLPEKGPCLDDDIGGKCLKQRSKLYYVCKRCNSVIGKNKQMPHLEPEGCAHDSKYIHIKGKIPPPDSEGTSAAASEGESSSSTTHDGIPFYNFFNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNC9</accession>
    <name>A0A5B0QNC9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNC9"/>
    <dbReference type="OrthoDB" id="2508582at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022816566" evidence="2">
        <location>
            <begin position="21"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="133" mass="14465" checksum="38632AF99E471644" modified="2019-11-13" version="1">MPSIISIMFFAILACGFATADLATDQKMTCAFHCPTLTEGGCVRNKGRDKKGNVNKWEVVKAFATLGYAEYYNCLGLNADLSVCAEMGKIVLPPSPQPQVMTINSEKTRKELGEDSKPFDITAGDPEECSYTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQB3</accession>
    <name>A0A5B0QQB3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035153</name>
        <name evidence="3" type="ORF">PGT21_036002</name>
        <name evidence="4" type="ORF">PGTUg99_010250</name>
        <name evidence="5" type="ORF">PGTUg99_028901</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000212">
        <property type="protein sequence ID" value="KAA1123032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQB3"/>
    <dbReference type="OrthoDB" id="2503819at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1123032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1126360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="311" mass="33107" checksum="07E76FD2EDA6E49D" modified="2019-11-13" version="1">MGFTKSSSKSSNAHNAFMGRNRRDIDSHHFLGPIKSRVTYFNGSMTWMKGKALVKNGEEDLFCEFEGDPNFIERRFGGNVKRPREKTMDEDHVSEEEPNGTQPEPKAQPTPETAVNNQPNKENKVAAAGDVPTPKRSRVSDPLGLGQRTLADAILQGPNEGPSRNTRSRAGSSLRGKGGHRTPLVTGTSRKGSGSTARKMSTGKAVGRKGKGKGKATDPDQLAEITAGIDGFSVFSNHQIEEDPANPNNLAVAGPSTSKMAGMDAGEWAHPVTHSAKVGVWLERTAEAGSQAPVDAPRSRAPSSIAGIVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVV1</accession>
    <name>A0A5B0QVV1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004986</name>
        <name evidence="1" type="ORF">PGTUg99_009789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000313">
        <property type="protein sequence ID" value="KAA1105032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVV1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="263" mass="29984" checksum="4F1BF3DF54A76977" modified="2019-11-13" version="1">MMDPKGQYNDLYVKLRNKGGLSYTLAPEHTSWLTSPDPFNKGPHPYYLVGSDGITMEFHFKDALASRKAVDIDTQFEGTRNDLQKLLSQILQSSKEETSDPASYYGQMTRKFRENAEELNSIHEKILQSASLPHGKNADQLNNLQTILKETPLLTEIPKEMKGTEKIKLKEHAKTLQALIFQTIDFLYQKKLIKLDEFEGFFREGNTMQRLANHLMLISGGNFHTNLQIPFKLGSSEKIQGLITGFLNGNSKEFMRLAFPKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWD6</accession>
    <name>A0A5B0QWD6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NOP16_2</name>
        <name evidence="7" type="ORF">PGT21_021045</name>
        <name evidence="8" type="ORF">PGTUg99_037116</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOP16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWD6"/>
    <dbReference type="OrthoDB" id="285729at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019002">
        <property type="entry name" value="Ribosome_biogenesis_Nop16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13243">
        <property type="entry name" value="HSPC111 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13243:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09420">
        <property type="entry name" value="Nop16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1097815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1117223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="308" mass="34849" checksum="B7D1DC470FFA9A82" modified="2019-11-13" version="1">MANPRQRRKSRSGAPKARLSKFANSKLKKVTPRNFPGPLSNAYNPRQTPRQNYERLGLLSGKLAPRLAGGIEKNTDLQNIWLNKYKKKDEFEILRTTSDGEVVDNEEDENDEEETDEDDSEKSDDDGNQATTVNRDPKLSKGEGRIIRDADGKIIKVIIGDESEIELEPTAEDPSKIVIERDLQPSTSTPWGNPLDTPSYEQDPSVTHHPPAPVLQGIGYQNPRQGFVPPKTKFVEDLEKISSNRLATYSETIKKKQHLSENQTNWLLKLIEKHGLENTHLMARDLKLNKDQKTEGEIRHLISKLASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX59</accession>
    <name>A0A5B0QX59_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QX59"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="EMBL" id="KAA1117897.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
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    </evidence>
    <sequence length="515" mass="57304" checksum="338667B787200FB8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="8" xmlns="https://uniprot.org/uniprot">
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    </evidence>
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            <dbReference type="Proteomes" id="UP000324748"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
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                <person name="Schwessinger B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <comment type="caution">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="199" mass="21259" checksum="6B36358A213BF830" modified="2019-11-13" version="1">MAAVGTRENLRSRIASEPQELEILIEIATGTGIGMDETKTENGIVNVGETTSIGTLIGIAIEQGTETEDKITQKVMIGTRIETEIVIVKELVMEMASTETQIAQSGILLVIANTGTGTETAQEAQTAGPGSLIVLATSHAMDVVAPALPVGRRVQVGAVVVEKGEIALARRTLAKEVPVTRWMLIQTLMMKTKRQKWPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R362</accession>
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                <person name="Schwessinger B."/>
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                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        </location>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="120" mass="13655" checksum="EAF3E19AEAFF06BA" modified="2019-11-13" version="1">MLFTSIVFAFYLLKYQGTSAHPTLYTKSLVKRGIDQEATIGTQIHLLHKRMEGSYKEAKVKESRIGCLHKMLERCPKTGEKKETNEVGEELNKNSHNVYSSSDKDKIVHEKSYEGSSSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3V4</accession>
    <name>A0A5B0R3V4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Putative RNA helicase</fullName>
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    <gene>
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        <name evidence="14" type="ORF">PGT21_031975</name>
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        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3V4"/>
    <dbReference type="OMA" id="IMIFTDT"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17955">
        <property type="entry name" value="DEADc_DDX49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="51"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="306"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1119695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="502" mass="55707" checksum="B07CCC0416097098" modified="2019-11-13" version="1">MDILNSSASNGLKTNLSKPASFASLGVQPDLVAALTAMSITSPSEIQQKAIPDILAGRDCIGSSQTGSGKTVAFAIPILQALAKDPISCFCLILTPTRELAFQIGSQLKVLGSLAVPGFTYSVIVGGMDMMSQALDLNKRPHIIIATPGRLVDHLKSAEGELGRWNLRRCKFLVLDEADRMLTPTFAESLSYLINEQLPPPTDRQTLLFTATITEPVLALSEKTPPAGKQKPVVHLCGPSRINLPATLKQFYSFIPSQVKDVYLYYLLLNVCDLTGLPPPPKRPKATKSKRVKYSNKTKRLINEDDEPDPVSNLPQTIIFVSKPRVAEQLHLLLESSHLPYPIKSRSLHSNLSQMQRLDAIREFEERQVPVLISTDLGSRGLDLKQVKLVINWNLPLDPNEYVHRVGRTARRNQGGEGDGVSISLVAEHDIECVLKVEEQIGSKLELIPLKEEKVLEYLNPISKAKREAELDLNEMKFGQRQLINKKKQQKLDKIRKAEQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3Y2</accession>
    <name>A0A5B0R3Y2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Palmitoyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">YKT6_3</name>
        <name evidence="4" type="ORF">PGT21_037174</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3Y2"/>
    <dbReference type="OrthoDB" id="27923at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15867">
        <property type="entry name" value="R-SNARE_YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045848">
        <property type="entry name" value="R-SNARE_YKT6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF1">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="73"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1120119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="143" mass="16142" checksum="618543BD22B00F80" modified="2019-11-13" version="1">MAKRNPLGHREYTLSRPMETTSAVEVYTGEPDRGHHHRQRISAVNNPSSPPNLGFTAVNEYLVKYQDPKQADTILKVQQELDETKIVVHKTIESVLERGEELDSLVDCSNALSSQSKTPQRPRAASWPWRVYTSMAEVVSLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R405</accession>
    <name>A0A5B0R405_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial</fullName>
            <ecNumber evidence="10">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Succinyl-CoA synthetase beta chain</fullName>
            <shortName evidence="10">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_033104</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="10"/>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R405"/>
    <dbReference type="OMA" id="ITACDEV"/>
    <dbReference type="OrthoDB" id="1552at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000004">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF1">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="36"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="354"/>
            <end position="356"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1119745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="419" mass="45174" checksum="38F8A2698CB3AC3E" modified="2019-11-13" version="1">MYRFPARPALAKSLNRVILGSKVQTRQLSIHEYMSMGLLNEYGIPTPISKAAKTPQEAHKIAESFGKPMVIKAQVLAGGRGKGHFEGKDGLKGGVQMVDTPEQALQYAKQMIGHKLITKQTGAAGRICNAVMLAEKREPSHEYYVAILNDRSLNGPALVASAQGGMNIEEVAAKDPSAIHTFPIDFQKGLSKQEGVEIAKTLGIKDHSAAADIFINLYRLFKDKDATQIEINPLAETKDGAVLCMDAKLGFDENAEFRQEKVFQLRDRSQEDPTEVEAAEYGLNLIKLDGNIGCLVNGAGLAMATMDVLKLNGGNPANFLDVGGGATAEAVKKAFELITSDKGVKSIFVNIFGGIMRCDVIAEGIIKATNELSLDIPLIVRLQGTKEKEAKKLIKESKMKIFAFDGLDEAASKAVEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R9I7</accession>
    <name>A0A5B0R9I7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036569</name>
        <name evidence="4" type="ORF">PGTUg99_028240</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R9I7"/>
    <dbReference type="OrthoDB" id="3189033at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="414"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1095204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1121474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="530" mass="58469" checksum="ED344EA9CDE954E9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RTQ4</accession>
    <name>A0A5B0RTQ4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenylate cyclase</fullName>
            <ecNumber evidence="3">4.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP pyrophosphate-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Adenylyl cyclase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CYR1_1</name>
        <name evidence="17" type="ORF">PGT21_011936</name>
        <name evidence="18" type="ORF">PGTUg99_006691</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="18 20">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adenylyl cyclase class-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000139">
        <property type="protein sequence ID" value="KAA1128732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2021138at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RA_CYR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenylate cyclase AcyA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenylate cyclase AcyA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
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    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23010">
        <property type="entry name" value="RA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="18" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="695"/>
            <end position="798"/>
        </location>
    </feature>
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        <location>
            <begin position="1414"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="14">
        <location>
            <begin position="1766"/>
            <end position="1902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="507"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="630"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1171"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2055"/>
            <end position="2076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="23"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
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        <location>
            <begin position="188"/>
            <end position="208"/>
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        <location>
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        </location>
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        <location>
            <begin position="430"/>
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        <location>
            <begin position="517"/>
            <end position="531"/>
        </location>
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            <end position="1183"/>
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            <end position="2072"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00005381"/>
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        <source>
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        <source>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00032597"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00032637"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50200"/>
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        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1089262.1"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="KAA1128732.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="2160" mass="234387" checksum="FEA78648FCB74013" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RTS4</accession>
    <name>A0A5B0RTS4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosducin thioredoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010954</name>
        <name evidence="3" type="ORF">PGTUg99_013043</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1128758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosducin-like_reg"/>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46052">
        <property type="entry name" value="PHOSDUCIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46052:SF1">
        <property type="entry name" value="PHOSDUCIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="330"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
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        <location>
            <begin position="45"/>
            <end position="54"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="70"/>
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    </feature>
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            <end position="148"/>
        </location>
    </feature>
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            <end position="164"/>
        </location>
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            <begin position="202"/>
            <end position="211"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="46350" checksum="01610ADA325AD4E1" modified="2019-11-13" version="1">MPTLEQAALSGTLTSIGLPQERDPDGREPDRSQEECTLEDLLAINDDDDYDDFDLPQRNRAAGGDGYGFLNTNSPATHNPRSTSPAGSQSMSRGWSGNTGPKGVLLDFKASHGKSGAILGGGNTLKPNYPRQISMSSALASKRSNPHNGNRPSSPASDSDSSNSSDRDHQIRTNTALCNSTKTTTRLIFSDNHQQHRRPFGSSSLPSSSPSAINNNPRVDARGRKLFGHLREVGVENFIQAVEAEKDDKETVVLVHLYDPALESCLILNNHLSNLARVHPRTKFLRALASELDFFNETPAANSSNVGVGIPTLGTSPVRGFHSLNLMDDDPFDDEPRLSSDHHHRHHDHSDDDHPLEGRTCKATDSDILPTLIWYRSGEYVQSLPALERELPLGGIVRGEQGCRDLQRLLRKNGIINDPESVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S4K1</accession>
    <name>A0A5B0S4K1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_004014</name>
        <name evidence="7" type="ORF">PGTUg99_017984</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000077">
        <property type="protein sequence ID" value="KAA1132712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S4K1"/>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07087">
        <property type="entry name" value="ALDH_F3-13-14_CALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="31"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1073212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1132712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="576" mass="63908" checksum="A9D74E55395ABCB9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S4U5</accession>
    <name>A0A5B0S4U5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011991</name>
        <name evidence="3" type="ORF">PGTUg99_037355</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S4U5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="85" mass="9116" checksum="1A96960454C935FC" modified="2019-11-13" version="1">MSPQPPLAVTAISADKVTKASLTLETARSTASTKPINKTWTKSARTSTQEHISPEVVPSDWDSSLEEDLKAEKPKSVISAAPKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLU9</accession>
    <name>A0A5B0LLU9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">rRNA-processing protein and EBNA1-binding protein ebp2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EBP2_2</name>
        <name evidence="8" type="ORF">PGT21_018927</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLU9"/>
    <dbReference type="OrthoDB" id="443772at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008610">
        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05890">
        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="590"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="477"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1064903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="697" mass="78096" checksum="3D4ECE5FC2AA8AAD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMG0</accession>
    <name>A0A5B0LMG0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMG0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="128" mass="14356" checksum="C354270B0F1BC336" modified="2019-11-13" version="1">MNTFSHRFKISTHALVDGLKELDNLSQKTGSHYLHHESLNHLSSSSQLPLTSSDPILNPFHAHSTVKLGKLLDGDLKGLDEEKVITFLELDSDYHSKVSKLRKKREIWFEVVDAAHGSLKELLANQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMZ8</accession>
    <name>A0A5B0LMZ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008828</name>
        <name evidence="4" type="ORF">PGTUg99_006831</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000018">
        <property type="protein sequence ID" value="KAA1136886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMZ8"/>
    <dbReference type="OrthoDB" id="10284310at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1136886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="381" mass="43963" checksum="FCF037A8FD9B850D" modified="2019-11-13" version="1">MRQEAEQVHEEVLPAFATGFDRDPVNSHNPSSSESVTSGKITEAQSAAFLEQEKNEQTGPPIAIKSQPTITQKQLEGSLEWKQRRQALEQEFETIREQLEMFKKKIGNVPWIKVYRHTDLRTAIRYIKEERRLMKNLRKLQAILELIRLCKRDRLFKLTDKRIVLAPLENVDLSTPKTSMRSVRQLASKAISTRRKLAKSYKAQGKSSTIPVRNINAQQKPLSQNSGTEVSLMGSSLEAGKLTGNALDAVMDRVTWGWIERLNKLVHDIMPIETAVEMIESQTNNGHLEGFKPFLLQQFVFRTIKFIYKNGMIPSNRLKDFLAFKDTERLALMNVYESTQTLPPIVIQYLEAVFGFDYPIFFLESVIESNHEFYRYLRQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LN71</accession>
    <name>A0A5B0LN71_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cysteine protease</fullName>
            <ecNumber evidence="1">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ATG4_3</name>
        <name evidence="4" type="ORF">PGT21_011761</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LN71"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1 4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="3">
        <location>
            <begin position="117"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1065825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="206" mass="23359" checksum="C93F7242DA0B2285" modified="2019-11-13" version="1">MVTINSLEVELLMVNPSYHPLFTNQRAFMMTTNGTINVKLLGTSSSQDRSRTGSSPFKPSPEKHKHDLQPDLLLINLRRSLQHPTKNSDKVSFQTEHSSSTSMNLRREQLDDFYAGAYPSSALQTFHPEKFQRRKLIALDPSMLLGFLVKDEADWEDLTAWIQPFKPPLFHIAEAMPWMCTNIDSTTSQTSANFRPSDDSDLGIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNI3</accession>
    <name>A0A5B0LNI3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001147</name>
        <name evidence="3" type="ORF">PGTUg99_004879</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1079922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNI3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="301" mass="33468" checksum="6D48F0A5D01ACD4C" modified="2019-11-13" version="1">MAIDPLQSISNTISQPSQNPNPNPLNTNSSSTNPLQPTTVPQSGISTPIPKQPLALIPSAPSKSNPHQRAAETRLHAQLNPGQIQMDNGSLPKGTVSTSKVPKMANILNAETSTPIIAEPAALKVKARNMLLTKAIKAQEDGNDNKAECFFKMHDILLKEEATVIAHSNKEIQIVTGPAVVPQKRPTAAGGTTELRGFKFKWGVSNSHDNGSFTPYFHKNISELKGLIPLTIFHRKWQEEDLSCHSRNRPKTDETTAEKGLQYHSLPVPDEWTQFFAHWTLNHRCFYETMRDRYQLHSRMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQ45</accession>
    <name>A0A5B0LQ45_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 8 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RPT6_1</name>
        <name evidence="14" type="ORF">PGT21_031535</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQ45"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000006">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000044">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 14" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="182"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1066485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="407" mass="45007" checksum="4005C5F97FCD3AF5" modified="2019-11-13" version="1">MPSLVAQPPSSSQSNNQGGIGSYYQTKTEYLQLAVNRKTQNLRRLEAQRNSLNARVRLLREELQLLQEPGSYVGEVVKVMGKKKVLVKVQPEGKYVVDFAPSIQLSQLTPALRVALRSDSYQLHSILPSKVDPLVSLMMVEKVPDSTYEMVGGLDKQIKEIKEVIELPVKHPELFDALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCRFIRVSGSELVQKYIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSSRGESGSGGGDSEVQRTMLELLNQLDGFEPSKNIKVIMATNRIDILDSALLRPGRVDRKIEFPPPGPEARVSILRIHSRKMSLTRGINLRALAEKMGNCSGAEVKGICTEAGMYALRERRQHVTQEDLELAVAKVLKRNLDASTSTTKLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRB1</accession>
    <name>A0A5B0LRB1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">20S-pre-rRNA D-site endonuclease NOB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NOB1_2</name>
        <name evidence="13" type="synonym">NOB1_1</name>
        <name evidence="13" type="ORF">PGT21_011151</name>
        <name evidence="12" type="ORF">PGTUg99_021561</name>
    </gene>
    <organism evidence="12 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="12 15">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the synthesis of 40S ribosome subunits. Has a role in processing 20S pre-rRNA into the mature 18S rRNA, where it is required for cleavage at the 3' end of the mature 18S rRNA (D-site). Accompanies the 20S pre-rRNA from the nucleus to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NOB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1066972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRB1"/>
    <dbReference type="OrthoDB" id="446759at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000020">
        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.10">
        <property type="entry name" value="Nin one binding (NOB1), Zn-ribbon-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017117">
        <property type="entry name" value="Nob1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036283">
        <property type="entry name" value="NOB1_Zf-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014881">
        <property type="entry name" value="NOB1_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08772">
        <property type="entry name" value="Zn_ribbon_NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037125">
        <property type="entry name" value="D-site_20S_pre-rRNA_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144206">
        <property type="entry name" value="NOB1 zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ribonuclease PIN" evidence="11">
        <location>
            <begin position="10"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Nin one binding (NOB1) Zn-ribbon-like" evidence="10">
        <location>
            <begin position="397"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="130"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="216"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="542"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="551"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037125-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1066972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1083910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="588" mass="64524" checksum="838D6DF3BAFDCBBF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSV0</accession>
    <name>A0A5B0LSV0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_008137</name>
        <name evidence="15" type="ORF">PGTUg99_023154</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000377">
        <property type="protein sequence ID" value="KAA1092226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSV0"/>
    <dbReference type="OrthoDB" id="432881at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140575">
        <property type="term" value="F:transmembrane monodehydroascorbate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08761">
        <property type="entry name" value="Cyt_b561_CYB561D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045150">
        <property type="entry name" value="CYB561D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422:SF45">
        <property type="entry name" value="CYTOCHROME B561 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422">
        <property type="entry name" value="OS05G0565100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="13">
        <location>
            <begin position="61"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1067521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1092226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="267" mass="29670" checksum="E4A52B63C85E8DE8" modified="2019-11-13" version="1">MAEETRANQFPPEEETPLLANQAEQSSTGPTAVSQDAAIMPSTDQRPKYSIASLLEAHRLPTHRTGMVWVAELAIWIWLLTVWVTVLTHQAGIFTFHPLFQSLSLFCFYQGIIILQPTDTPASKRTGLLVHEGFQLVGSLSIIIGASCIFYNKMSHSAAHFTSWHGLLGFISTCIVLVQALFGMLIGFETSRDYILGDSFGRKLWKFHRASGYLLIFLMTITVLTVTKADWVVMVSSKWSIWVLSISPIVALIGLLSLANPKKVFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWB7</accession>
    <name>A0A5B0LWB7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014255</name>
        <name evidence="1" type="ORF">PGTUg99_036794</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWB7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="158" mass="17950" checksum="184281CB508C093F" modified="2019-11-13" version="1">MLDIITSVLRFAFSYHARVSGLVRLYSLCGASELEIKFFDGIHRRVIVHLVYLNVIANVSKRTEKLPVLSINQPDFAVRTTIDPNCRNGSTARSQSSSRRTSCATTTYIKVSEQQQEMAVTVTILDEADYFMHSIGYENIRVPGQTLALYQHKSYPDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWH9</accession>
    <name>A0A5B0LWH9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWH9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022818452" evidence="1">
        <location>
            <begin position="30"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="141" mass="12851" checksum="4DDA8424B455A6A3" modified="2019-11-13" version="1">MQFNTLRLALSAALLSATCIISAPAPTLGLGIGGGLGIGIGLGGGLGGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGLGYGGGLGYGGGLGYGGGLGYGGGYGGGLGYGGGAATISLSDGRLHFPTYHRRIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZU6</accession>
    <name>A0A5B0LZU6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZU6"/>
    <dbReference type="OrthoDB" id="10459250at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="207" mass="23090" checksum="7F6C7D53B3DE6397" modified="2019-11-13" version="1">MNLVNLEETAKLIQYYLTLLASEKAQRAANYQPSDWELLTPDKQRARALKRVEIREDEKIANLLLWNSVKQDHKAKRRQLLAKLQKKRLAKMSNTSNSRAATTPAARTTPVAETAANSPITGQTIQTSTAPYPQPLDRGAANQLEERVNRLKLITIQKKMAPVLVERPTEATLAKNGSTPAVETPATATVPRTTFQLPRACKKKVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0M5</accession>
    <name>A0A5B0M0M5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_032515</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0M5"/>
    <dbReference type="OrthoDB" id="3255580at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1069743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="32870" checksum="D8DA93251CA32647" modified="2019-11-13" version="1">MRRPHHPPKRSRRESSPNTASTSVSDGAAAAATDPIDVDEEKLGESQVTPSASGGPAGANTDQEELSRAQRIARTAVSTSYASYNVPELSNQLDKNGRRMIAYPCKLCGKRINRPTSDSSCSNLLKHAASCVKKKHDQDHTRTLGSLGVTGTGDIDPREVTQKCAIWCAEAARPFSALEDNSLKSILHPTVLKNLPNRRMVSKAIHVLYTVVQEAFKLELKSHTGALYLGLDAWQSPNGFDILGIVVYWLNERAGLQSMPLDFIKLSRSHTGKYLAETVQLVVEKFGIASKITHPIMKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M109</accession>
    <name>A0A5B0M109_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH APSES-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_012613</name>
        <name evidence="7" type="ORF">PGTUg99_030981</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M109"/>
    <dbReference type="OrthoDB" id="6718656at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000001">
        <property type="entry name" value="APSES transcription factor (MbpA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000238">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.20, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="5">
        <location>
            <begin position="99"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="451"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="570"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="231"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="621"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="869"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="313"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="633"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1070455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1089946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="955" mass="106147" checksum="CE5815C2E257A9CB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1A7</accession>
    <name>A0A5B0M1A7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033933</name>
        <name evidence="3" type="ORF">PGTUg99_013304</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000072">
        <property type="protein sequence ID" value="KAA1133254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1A7"/>
    <dbReference type="OrthoDB" id="10288525at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137125" evidence="1">
        <location>
            <begin position="22"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="251" mass="27266" checksum="F8CBE192C9711B5D" modified="2019-11-13" version="1">MRAHHVFFIAVLGLIPGPSSQQISVITPKCDKNANVKFNPENCKTALKRVLYEPGNILDRVETHVERASGSCVIVFSSKGKVPSPISKDSFELSINKILTQCKDTAGSIPHPTISGVTFQVRPRGKDPVYEEDFPAEKPACYGVADARKSAAGLKVDCNVAYGAIFSYNDRSFSLDGYNPTNVARKGVGICQVQVFTSDGSIIKASIKQLDPIFKKMTAKCDTRYGAIISHTGATGRNGRVFLRTVPINKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1L1</accession>
    <name>A0A5B0M1L1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1L1"/>
    <dbReference type="OrthoDB" id="2507835at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="549" mass="58954" checksum="D645D8EB360B5E08" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1N6</accession>
    <name>A0A5B0M1N6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1N6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="71" mass="8030" checksum="9F07AF264E212C6F" modified="2019-11-13" version="1">MSPNTVYQNWIDLRSNPDLSSDQDWLEQSNLSRVGTWYCTDTISISTTTTHPTTLLPTEQDPTNTTTTLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1V8</accession>
    <name>A0A5B0M1V8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Superoxide dismutase copper/zinc binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1V8"/>
    <dbReference type="OrthoDB" id="2015551at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022668577" description="Superoxide dismutase copper/zinc binding domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19246" checksum="4D795C046B9B1C7E" modified="2019-11-13" version="1">MAHTIATYIFWSLLIVGQAFSLSSRRFRVGEHGHFDARLVTIQPDRRFLPITCTNIRSLMGIAQTALLHYKVPTPKPSCTSGNQKITVNQPSSQRVMGNLPGLVDVFTLRINDNLIDLTGPNSIIGKSIVVHWCEPDSTCMRQHRTLDPWRQGLKKYFRSYHSTPIDSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6A5</accession>
    <name>A0A5B0M6A5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Anaphase-promoting complex subunit cdc27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CDC27_1</name>
        <name evidence="5" type="ORF">PGT21_011545</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC3/CDC27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6A5"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="755"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="824"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="893"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="97"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="404"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="486"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="586"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="518"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="534"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="597"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1071558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="973" mass="108706" checksum="CF5646E3BE33FFF8" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8Z9</accession>
    <name>A0A5B0M8Z9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8Z9"/>
    <dbReference type="OrthoDB" id="10390437at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="142" mass="16072" checksum="E13866252325CABE" modified="2019-11-13" version="1">MADHQSRLAGWSTAHGYEPTALPAMPTKIDALHITFWGHSDINEEKIKQIKRQQSLHNCKRQPRKRYEDDVTHNLLLGLNSSEQLRMAPGYKYYSGIAIGAAYIFSSMRPLPSSGNRHRPSMRRSEKSLAKSSTRADGLYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9D3</accession>
    <name>A0A5B0M9D3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034057</name>
        <name evidence="2" type="ORF">PGTUg99_012667</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1072976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9D3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="407" mass="44421" checksum="676B83C0F1E682C7" modified="2019-11-13" version="1">MKANPLVLFGTSLQVRPTDRPQLLATQPPPPHQSTLHNPPSQRSRRLHIIKKNTSMESPHYTSSGSALQAQYQPGTDQPPTFSYQLEPYHDPQYLPPNLRFGIQEDPQYAPLNDRVGFCQDRYRNHQYEPSQDPRYPPNNQQADDYSSYDCNNEHVPGSYLSHTSPNIHPNHPDHRHGSPRYADMELNLERDRVGYQDPPSHSRQHYRPQATLANIIRHNNPRSHATPHLSPAIPPNSVYSTGTAHTMGTTTSRMPDAPHDLVPSTLPPSGSSHLTVGNDGNDPDTFPGSSRLTVGNDGNNTGTLSGSSHLTVGNDGNNPGTLSGSSQLTVGNNTNNPGTLSGSSQLTVGNDGNNPGTLSGSSQLTVGNNGGTFDSTSLTDDSLPNDPPPFSYRWQLAKRSPPFSYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB80</accession>
    <name>A0A5B0MB80_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014849</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB80"/>
    <dbReference type="OrthoDB" id="674948at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40129">
        <property type="entry name" value="KETOPANTOATE REDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40129:SF2">
        <property type="entry name" value="KETOPANTOATE REDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="346" mass="38793" checksum="AA271D77FE7FF6DA" modified="2019-11-13" version="1">MVTIEPINISTTNNNQGEPVDILILGAGWVAGFLISYIDQHKPTITYRTTTTKGGGEYQSIPFKFDPNHHTPDTDDVNQFRNLPHARSVLISFPIKDSGASRFLVSQYLLSRRTRRKTSTLSSTDQWIFNVNFIQLGSSGIFDGSPTLKAIDKEEGKQEGSVWIDRDSEYDKTNPRAISEDELLQMNGTKPISDEDVSVGTTVLNLSGLWGGTRSMRRYVGKIAATKEALERKTSLHMIHGLDVARAILAVVESFDLAAGQRWILTDQRIYDWWDLASAWGESSIDHPHSHLTIGPQADWVNQLMQEHNVRALPRSPALLGRAIDSRQFWTTFGLTPVRARMEDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFF8</accession>
    <name>A0A5B0MFF8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFF8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="11581" checksum="C7820F78B149F290" modified="2019-11-13" version="1">MYRESLRNLENQRQMVDIERQRIQNQHLRNQVVSDLQSQEQELRLRRELELAGSGLYKYYAIRVDEVVQPRRPGFSVSRAYALCVLTVISKGLRGEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFU7</accession>
    <name>A0A5B0MFU7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027771</name>
        <name evidence="2" type="ORF">PGTUg99_018691</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFU7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="189" mass="20783" checksum="8825D6EC1FFF3CE0" modified="2019-11-13" version="1">MFLLQVPLLVITFVIIGPIFSIPMGHEMAGEGSASRETDGKCHPGPQTYQVIHPNLTPNGASTNWEIHIHIGDLVGRTKEDPVRLNSHLKPEKLAHSQSSISIADENITHGITYANLPDAVNEASKTSCERQELLFRLNVAAVLPAFIGFGFIFTNINTCDPMLSFMWALFGSLYIYAANYLATKIAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MG28</accession>
    <name>A0A5B0MG28_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Karyopherin beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">KAP95_3</name>
        <name evidence="8" type="ORF">PGT21_035188</name>
        <name evidence="7" type="ORF">PGTUg99_036506</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MG28"/>
    <dbReference type="OrthoDB" id="2677826at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1076157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1091526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="242" mass="26017" checksum="D8B1475304905AFD" modified="2019-11-13" version="1">MSFSAQNPGGYPGIPRDTRCQEATPPGGTPPGERLQASSLGGVPPGKLDCVPACWEESLPASWYTDQLVGRTPSRRAGFYKIKDGLIQLLADEQRAVRQVSGQAIAVIGAVELPLGMWPGLIGQLLQIINNAEGGVPLRQATLQAIGYLCESTLPEVLAAQSNEILTAVVSGARKEEPSTEVQLAAVNALYNSLEFVRANFDREVRSYTIFSAVPACREEPLPTSWFKNQLAGRNRSQRAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJH6</accession>
    <name>A0A5B0MJH6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Splicing factor subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004199</name>
        <name evidence="4" type="ORF">PGTUg99_019295</name>
        <name evidence="5" type="ORF">PGTUg99_023825</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SF3B5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000077">
        <property type="protein sequence ID" value="KAA1132724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJH6"/>
    <dbReference type="OrthoDB" id="274726at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009846">
        <property type="entry name" value="SF3b5/RDS3-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017089">
        <property type="entry name" value="Splicing_factor_3B_subunit_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978:SF0">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07189">
        <property type="entry name" value="SF3b10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037010">
        <property type="entry name" value="Splicing_factor_3B_subunit_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR037010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1132724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1135585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="86" mass="9712" checksum="D8EF9D242E32F998" modified="2019-11-13" version="1">MSELRYTANTQLEQLHARYVGTGHADITKHEWLTHQHRDTCASVVGHPALLTYLAIAEGEKIGRTKFELCERMLQPCGLPPPKDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJS3</accession>
    <name>A0A5B0MJS3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJS3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="112" mass="12044" checksum="86AC9B26C2C3E5AE" modified="2019-11-13" version="1">MMTLNQMVTKDSALPAKVDHPRLWTLRMIDEGVPLQNRGTLPVPHSNAQSPHQSSPAPQAPDDNAPAPQVPDANSPAARVPDANAPAPQVPDIDNDELDSDWVQLDDDLGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKG4</accession>
    <name>A0A5B0MKG4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKG4"/>
    <dbReference type="OrthoDB" id="2503459at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="290" mass="32734" checksum="55253522173F668B" modified="2019-11-13" version="1">MARSSVTPRGTPSRLPSRQSTRIVTPTTSSSYVRPSRDLQRSVGPTSQSQQKGNSLDSESEAESVVQIPSNQQSKKKRTRKPPSTKQHKRARSSITTNTDDDSDEVDGVIDHTQDSDKENSKVQGSSKKKTSNLDNIREYFEAPYHAEQTDEDEGEKLSFKCKWCSKPYKKGLGTNSNLLKHRDGTKKSAPCSGRLAAIEAGCKLPLSKKDIAKKDNDRHQTKVKGSLTHAAFDNRVFNQLLLIWLIQYSMPWKRFDDFLLQVTFNYVRQGVSIYCSTWAATEAHRLYMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNE5</accession>
    <name>A0A5B0MNE5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNE5"/>
    <dbReference type="OrthoDB" id="10288023at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022891647" evidence="1">
        <location>
            <begin position="26"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="106" mass="11780" checksum="B37C14712744A621" modified="2019-11-13" version="1">MKSTITQSLASLILLLLISCGFALCQVGTGYDAMCEKHNPPMLPYCFDYYKFSGTFPSTERTADWEFSRGRPLFACPIGEWTLCCQRRNGHALLKTCDNAPKATGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSM6</accession>
    <name>A0A5B0MSM6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022447</name>
        <name evidence="3" type="ORF">PGTUg99_006507</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000446">
        <property type="protein sequence ID" value="KAA1078840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSM6"/>
    <dbReference type="OrthoDB" id="2507299at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1078840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="511" mass="57326" checksum="21FB48B68142D9C2" modified="2019-11-13" version="1">MDQTNPASQATTLFLDPLISSTATSGLNQTLPATNPTRICSARSTQATLASSNGTSKTRNRKRKPSSNQPDDRTTSTAKKQHVNRNTQALQPAPATQVSQPTPSTQASQTVSQAQTQARPIPTPPIHMIQDLRDKSVTDLRGLELEFCKGRRLTQDIKDQLREITLDYQKKVHILAIQHEMHSELLFKWLGAYNQSKGPNRFNQFCSYGPEARQVFDSKELPPGERMQVVAEIWRSMDEEARLKYDDWEFVNSLRVKMGLKPLKDGLPDDDDEDHNSVDDVEDSNSQITTAVTMKYCKKWAGVAVKQMNHMNAVHQVEGFFVLVSTDVQGTVFELGGSPLGESYIKLLQAKNDPFKQFRVWAAGLAAQARLTGLPVGPPKPMKKKRDAEGPWDLVDCHKNQTSMTDQLRDRLKTVTRGERSNGWPGTDTLETLTSLGVRCIIKPEATQLRIEDLCKPTKTMKAIPVLRVLEALHKGWFILEREEEVGTSNHSNSDQNRELVHQDSIGTHRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXW8</accession>
    <name>A0A5B0MXW8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Isovaleryl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_030814</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11 12">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXW8"/>
    <dbReference type="OrthoDB" id="9988775at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:3-methylbutanoyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01156">
        <property type="entry name" value="IVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000021">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase IvdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000003">
        <property type="entry name" value="isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000004">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034183">
        <property type="entry name" value="IVD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF18">
        <property type="entry name" value="ISOVALERYL-COENZYME A DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0274">FAD</keyword>
    <keyword evidence="4 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="15">
        <location>
            <begin position="65"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="14">
        <location>
            <begin position="182"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="13">
        <location>
            <begin position="288"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="215"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="299"/>
            <end position="302"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="395"/>
            <end position="399"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="422"/>
            <end position="423"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="424"/>
            <end position="426"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1081173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="49174" checksum="394C7822F6EB533F" modified="2019-11-13" version="1">MYTNYQQYIHRTTMATFNILRQTATQVLRTSSSKSSRFPVFLRKSQTGGFATQAYNEPIDLAGLNEQQLELQDAVRSFTRREIAPIAQKVDQENQFPSHLWTKFGEMGILGITVPEKYGGLDRSYLEHTLVMEEISRGSGSIGLSYGAHSNLCINQINRHGTEAQKTKYLPDLISGKKVGSLAMSEAGSGSDVVSMRLTAVKKEGRYILNGTKFWITNGPDASTLVVYAKTSPEKAHRGITAFLIESNFPGFSTHQKLDKLGMRGSNTCELVFENCEVPEENVLGEVDKGVQVLMSGLDLERLVLSGGPLGLMQAAFDVALPYVHERKQFGKPIGTFQLLQGKIADMYTKLNASRSYVYAVGRACDLGKVSRRDCAGAILYSSDRALEVATDAMQMLGGNGYINDYPTGRIFRDAQLYRVGAGTQEIRRMLIGRSFNEEYNDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYP2</accession>
    <name>A0A5B0MYP2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYP2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7386" checksum="2A274F4EE0254131" modified="2019-11-13" version="1">MIWNGGTGLEGLSVSKKPPRVPPEKDSVAHSSKVRSAFLIRPDTSWLTGSEALMEIGLEPVSVVDGDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7Y6</accession>
    <name>A0A5B0N7Y6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7Y6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="58" mass="6412" checksum="91B3C1A2D67C69EA" modified="2019-11-13" version="1">MAGQAWDDLPASLSSSYLPPTPQFPHSKSTTARTIAYRERSHNPIQVKIVTESLATRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8B4</accession>
    <name>A0A5B0N8B4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8B4"/>
    <dbReference type="OrthoDB" id="2518445at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="180" mass="21084" checksum="50EA57136E1E8E80" modified="2019-11-13" version="1">MPPFEGEGGKKRKRSRRPATSSSISLAKALELERQHALQDLCSNPPDDRPSNHTSRGLPDESNQDVNDFESDRQHYEDHQELDNDYESDTWVRLIADYFRSEDYKRRKLLEEQHWQEVYDKMFTSFHDCATKTSHWGNQMVWDTDWKAECECTLTRKRPVVLVEEKAGSSFVIASLTRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAM1</accession>
    <name>A0A5B0NAM1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">rRNA-processing protein and EBNA1-binding protein ebp2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EBP2_1</name>
        <name evidence="9" type="synonym">EBP2_2</name>
        <name evidence="8" type="ORF">PGT21_018260</name>
        <name evidence="9" type="ORF">PGTUg99_033847</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NAM1"/>
    <dbReference type="OMA" id="RETMFHR"/>
    <dbReference type="OrthoDB" id="443772at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008610">
        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05890">
        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="149"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1085753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1136158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="269" mass="29628" checksum="FE9FC25891DA6C36" modified="2019-11-13" version="1">MASLESLFSNPTATAPGGFGASVGLDFFETQLVDSQARTSVEDVNDDLKIEVELYKNALEAAQQAVRKFETSGKPFFRPSDYFAEMIKSDAHMETIRLRLVEEAEGLKASEEAKKKRELKKFGKAIQVENKLQREKETKRIKEGVKELRKNRKDVTKLDGQGEQEFEIAIEETLSGNPRKRAGGPSGSGKESEGRKKSRTGRERQFGRPKPKGVTGRRWKENTKSSAGSFEGMGGKGDGRISSGTGRNRGRGRGAGGSSKRGSHRSKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NB27</accession>
    <name>A0A5B0NB27_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025493</name>
        <name evidence="3" type="ORF">PGTUg99_022096</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NB27"/>
    <dbReference type="OrthoDB" id="2499492at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="308" mass="34281" checksum="E527B7FF8D306952" modified="2019-11-13" version="1">MVSRLKNLFAKSKSGEAKFSRRLDAIFCRGNRTSSSQSISSKNKELERTSSRATIDSKINVNNRRLQIRLSVTRTTRYSPVHCSPCSPPAEGTRLEQALPLLPTEIRKESETRRASDLPRSPSPIATSHESSSSESQSTDCHYFENVKSETSYGSSEILEAQEEAVRIKFRDAYFPAPSTTCTEKTNLKLSRNLMDALSSHQIEWAAMIELQKADHSGILRGLTSRQIRWLVREGRCKTWLVGTAGPLIEEEKDEVQDVQSIDSSDGANLTEPISEEAVVLCARKAISPMDQLPAVLGVRSTCTDSRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBC8</accession>
    <name>A0A5B0NBC8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002396</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBC8"/>
    <dbReference type="OrthoDB" id="10253553at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="8708" checksum="D75BC033133E9838" modified="2019-11-13" version="1">MESNKVNSSFFPPPPLRHRLFTNKNLSLAKQMRVGANNYQEISDQRPFNRSKQTEIVGEELNNSGPLRYTTLFDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDG3</accession>
    <name>A0A5B0NDG3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphomethylpyrimidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_030165</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDG3"/>
    <dbReference type="OrthoDB" id="10028886at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19367">
        <property type="entry name" value="TenA_C_ScTHI20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.910.10:FF:000003">
        <property type="entry name" value="Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000003">
        <property type="entry name" value="Phosphomethylpyrimidine kinase ThiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="6">
        <location>
            <begin position="13"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="5">
        <location>
            <begin position="304"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1087375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="56247" checksum="7550BFFCAF3FECF0" modified="2019-11-13" version="1">MQSINVLTIAGSDSSGGAGIQADLKTFTSLEAYGMSVITAVTAQNTTGVQAVIGMHPSIVAEQLESVVSDISVRAIKTGMLSSPEVIEAVALSLSKFYPDRTQQPHLVIDPVMVSTSGHELLGPGGAEILINKLLPKSSLVTPNLPEALILSGRKSRHQTVENVQDMESCCREIARLGAKNVLLKGGHILMSDDMVTDLLYQAETDTFKRFTHQRLETPNTHGTGCTLSAAITVYLGKEFSLIDAVEAGIRYVEGGIEHSFKIGKGSGPLNHFHNITQRLIPLPTDRQKYPFTSALIAATGLSWDVFTSSHPFLQGVRDGTLPIRCFQHFLRQDYIFLTHYARIHSLMALKSDEMNEIEATATIVKQITEESKIHIRMCKAWGLSEADFLRTAESNQTIAYTRYVMDIGHSKSLLHLRVATAPCLLGYGEMAMKLKSDPLTVKDESNPYWEWILHYADPGFQSAVALGREVLEQMVLRDPPSVTSFQTLKQIFKKAVELEIDFWQMGLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDL6</accession>
    <name>A0A5B0NDL6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_013562</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDL6"/>
    <dbReference type="OrthoDB" id="10296316at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="318"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="442"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="337"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="442"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="456"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1086853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="50800" checksum="4A209D500D855E91" modified="2019-11-13" version="1">MTVSRHNTNPSSNIIATSDISFATGEENFPSGLPIADQMTSPSNPDPPTHPISRAIADFKELMSGEDPSLVDFAVTRCFPSFFPSTSLPQDTNNLVLTSPTTTPIPMGSATDIPLGFLGSGETNVPPFMPSLDFGSNSFNCPVPSNSIPPQTFPNGSSDTTLNGPLLSGPIFVETLYCFPSYQTGMPLSPFSLPNFPEVDKTKPSRRAFGQSELASVPVAGPSSRPYIAPPMGLNFNFNQSVTNNSILDGWQPDVTLTDRFLGSELSNAPVAGPSSQPYTPPTMGVSYNFGDSVPNSIPYGQQVDQLGTESASEEIQNISKQKGKKRVYESPSKGYQSDRKFNVDRREKPPLNPEDRLRCTKDGHNTSFGTEAAWKRHNRIYHGAPQARPRARRQKASDKKYPCKYANQGCKKTYKSLNDWGYYNHLMKDHHEVVILTGPVRREMVSTSSEEHTPAPGPSREPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDW7</accession>
    <name>A0A5B0NDW7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_023730</name>
        <name evidence="9" type="ORF">PGT21_025215</name>
        <name evidence="10" type="ORF">PGTUg99_020948</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDW7"/>
    <dbReference type="OrthoDB" id="1913277at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039205">
        <property type="entry name" value="NDUFA11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21382:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21382">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1065069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1085949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1113789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="178" mass="18501" checksum="06C92D31DF577620" modified="2019-11-13" version="1">MGDIERRPPIFMGSLFLGQSSGQFKDYDYRPALGSATQLGLYTGLAGTIVGAINHGVISPSEANRGYITNVAKFGAMATAVGFAFGGVSQSVASIREKSDPWNATAGGCASGFLLGLRAGHLSTALGQCAIWGAVAGGVNYAGDTLIPANHGKSREQQQQERVKLLGSQKDPLLLNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFJ4</accession>
    <name>A0A5B0NFJ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Trehalose-6-P synthase/phosphatase complex synthase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TPS1_3</name>
        <name evidence="2" type="ORF">PGT21_029221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFJ4"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="194" mass="20684" checksum="E755AFE4E88AD72A" modified="2019-11-13" version="1">MTESNINNNLLSSPHPLRPFDLFTPSPSRPASAAATRLPNTRAHPSSTTARTIANSTTTSTKTNGGGSSSQTMPTLDANSSRLIVVSNRLPITISSHPSSAPPTKDIPQQSSTEAGKHHHKAGEGEEKNSYSFKLSSGGLVSALSGCKKQMTFSWIGWTGLDVPEPDKPGIEDQLCQLYSCKAVWLSDELADKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFP7</accession>
    <name>A0A5B0NFP7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034788</name>
        <name evidence="3" type="ORF">PGTUg99_028973</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000003">
        <property type="protein sequence ID" value="KAA1138283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFP7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1138283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="199" mass="22145" checksum="E3125BD56973079E" modified="2019-11-13" version="1">MTQLYWAMVDIHCGMRPTNHVLGKGPIFAQSDDDDSAERAGTDQTIPAHPSLAPKLVNMAPLASHGCPSRQTIGNSLAHLFAGQRSDPFPSMGSTSDLLTSLQQLVTQPAHDSCASEIQFHNDCLQSFKWQVEEHIRHHKEQRIHHVNMKALLSHQKEVIARHQEERSRASTQATELRSLLINLIAKFSGQREGSSKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGN8</accession>
    <name>A0A5B0NGN8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">mRNA export factor GLE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Nuclear pore protein GLE1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoporin GLE1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">RNA export factor GLE1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGT21_026244</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GLE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGN8"/>
    <dbReference type="OrthoDB" id="420884at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.510:FF:000003">
        <property type="entry name" value="Nucleoporin GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.510">
        <property type="entry name" value="GLE1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012476">
        <property type="entry name" value="GLE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038506">
        <property type="entry name" value="GLE1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960">
        <property type="entry name" value="GLE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960:SF0">
        <property type="entry name" value="MRNA EXPORT FACTOR GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07817">
        <property type="entry name" value="GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="278"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="46"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="117"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1087230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="687" mass="78761" checksum="43ECBC60CC05DA04" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI79</accession>
    <name>A0A5B0NI79_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_033975</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI79"/>
    <dbReference type="OrthoDB" id="65716at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053045">
        <property type="entry name" value="Zinc_cluster_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986:SF7">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="119"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="543"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="119"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="543"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="573"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="585"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1087579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="670" mass="74944" checksum="3E9B40B7CCB64EB7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIU4</accession>
    <name>A0A5B0NIU4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_010997</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIU4"/>
    <dbReference type="OrthoDB" id="2495158at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22271">
        <property type="entry name" value="DPBB_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF21">
        <property type="entry name" value="EXPANSIN-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023074464" description="Expansin-like EG45 domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="3">
        <location>
            <begin position="43"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1089235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="234" mass="25215" checksum="E7ED60E5C448216B" modified="2019-11-13" version="1">MLLQLTTLSLLSICSFAAAAGPPRVGTKFPQVQGTYWNGATYEGSCLLSTYQRAPPAGLEYVAVANNLNRNGDYCGACIKVTPLQGKRAPVLAIVSTYCADCPANALDMPGTMYDRLMGSAPGRPGIGKFSWEVVACPLKDALPKIKNKEGSSKYSLSMLVADAKFPVQSVEINSDKRVFPAYKRDYNYWEIHNSGPPLANTVDVKVTCTNTRSFVVKNVDPSSKDPFKAANGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIW9</accession>
    <name>A0A5B0NIW9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIW9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022896235" evidence="1">
        <location>
            <begin position="24"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8354" checksum="84F6FE58548867D5" modified="2019-11-13" version="1">MKLPKVVLALSLVLIPIMGGVLGTASTCTKEGCNGRLVTQFEDSSKSCSNCGRTHQARFLACETWTFNNPTLSLLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLN2</accession>
    <name>A0A5B0NLN2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_004870</name>
        <name evidence="11" type="ORF">PGTUg99_036682</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLN2"/>
    <dbReference type="OrthoDB" id="2496822at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="9">
        <location>
            <begin position="269"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1070241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1090157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="440" mass="49765" checksum="25279D15F21C50FA" modified="2019-11-13" version="1">MSGIWNNFVGLMPDKSILQHSTKYFFFLSLVLSAWTHPKYYHSRLARCLRVPLSLISATWWLCYPFLFLVEPRETKVSANIGNFAWTYVMMTKCLDWGLFSGPQHRRRYVRSSDDSGGAWEAAEKNLKDRLPPSSLIDQVLLQLTSMRGSQFPWGHFSKLNHNSLGCDMLKFLMCHIYSLTVLAGLLYMEKLSTTSTIQSFVFLGRNVSLPTLVFMARFMSTPILLTGLCASFEAMNSALAVLAHLTHPLLCRLGFPDVICEFADPVYYPPLFENVFAFNSLSEFWGKTWHQLFRRSFVVLGAFPLGGMTRALGFSTQAQKMAGFVGAFMASGFMHAYPFFLMADPTTSHGNVSRLEIFCVFLCFVVQSLGALIEPILIPLIPKRLGGGRLWTISFLLLTLPLFSMATNKPARLFSIFKPLDEWSIHYLFLPAVLTPIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPE1</accession>
    <name>A0A5B0NPE1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPE1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="57" mass="6402" checksum="A677723A95FD69B7" modified="2019-11-13" version="1">MFCSQPHQPTENNPFDRLNNIHLNQRRSSHAALTEGPRNAEACRSHPIIPGPEGTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVL0</accession>
    <name>A0A5B0NVL0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028304</name>
        <name evidence="3" type="ORF">PGTUg99_005673</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000146">
        <property type="protein sequence ID" value="KAA1127952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVL0"/>
    <dbReference type="OrthoDB" id="2499752at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1127952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="320" mass="35752" checksum="B1E138C1E151B9F5" modified="2019-11-13" version="1">MCGLISTHAPQSIVSHQPPPRFSDTPAATATTFPNYTRPNNSVLRPPLLSIPQLQSTTISAPSSSQSTPTSTSDYSPKLNKPPTPTREKRRKLRSTRAGSPVPASISFNASGLRPKNKQPFRSSRVQHHSEPCSDDEDYQPLLSFQRLNLKSSMPPVPPAIPQDLMFPPSQCSYAHTPIVEPSYTTPRDTPNDTYTCIRTVSNPFSVSISSQPPAGFTKSMPRLNRLNRSSISSSHDPLSSCLELRKSEEQDKWWHWAKVCKVTGDGNKSSHLAPLRCQDLKRNFDDHLDCDNYQEPGLRIQTRPFSESTIEQQKFYYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWI0</accession>
    <name>A0A5B0NWI0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MCCC1_2</name>
        <name evidence="9" type="ORF">PGT21_017441</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWI0"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF33">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE SUBUNIT ALPHA, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0092">Biotin</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="8">
        <location>
            <begin position="34"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="7">
        <location>
            <begin position="153"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1092894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="330" mass="36257" checksum="A44BB823962430F0" modified="2019-11-13" version="1">MNSGRAFSRKITSQAAFQGDVFDAWSSQVNSKPLFKKILISNRGEVACRIIRTARKLGVKTVAIYSDADRESRHVKMADEAYRVGPAQSTDSYLNIEKIVELCIRSGAQAVHPGWGFLSERAEFAEKLKQSGIVFIGPPSSAIISMGSKSQSKEIMISAGVPCVPGYHGKNQDPGFLKSEADKMGYPVLIKAIKGGGGKGMKIVFEPSEFQSQLESAQREGEQSFGDGTVLLEKYLLNPRHVEVQIFGDLHGNYVYLYERDCSVQRRHQKIIEEAPAPGLSLEQRTKLGEKAVAAAKAVQYVGAGTVEFILDSTGDFYYMEMNTSTRSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P490</accession>
    <name>A0A5B0P490_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P490"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="238" mass="25957" checksum="12EEE1E57F8B1FD7" modified="2019-11-13" version="1">MSDEGLYSDANYSASQHGIASILNQDFEQQPFYADHNFHPSQTQLASGLNHHETGSFNPDTNSSWPNNSSPVDGRGNTLTRQSTHMTQGQSPACTHPFSSQSTAIRQPLTQSFMNTQHINNLQNRQPSNGQPLSQHPLNSQPLTQASTGQLPNQHPSSRQPSNHHPSNHQPSGRHSSNAQPSNGQHPSTQASTHQLPNRQPSSRQPSSQQPSNQHASNGQQPSTQASTRQIPNQQPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7H2</accession>
    <name>A0A5B0P7H2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_004702</name>
        <name evidence="5" type="ORF">PGTUg99_034300</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7H2"/>
    <dbReference type="OrthoDB" id="1166329at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10902">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1097381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1134267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="129" mass="14408" checksum="C94755387761E096" modified="2019-11-13" version="1">MTRLYVKGRVLGYTRGKRNQKPTSSLVQLEGVSNKEEAAFYLGKVRKMDLCFTPDSILSTGLRSTVEHHHSDLAYITVAPSAKLLTFSLRIIPITNSESLMFTVPSEKSRARRSESFGAKSLDRTATRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P853</accession>
    <name>A0A5B0P853_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028397</name>
        <name evidence="3" type="ORF">PGTUg99_029392</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1108085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P853"/>
    <dbReference type="OrthoDB" id="2520628at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="282" mass="29807" checksum="718A11694C0EB218" modified="2019-11-13" version="1">MVHSAAPAFIQPVSALFDALATPIHPTFFPVACFPLLHAFRISVAYRTLNTRAGGKTSAAADLAGFLLMAWGGSIISHILLNLPIPQLLSFSPFLVYATTHMSLPGIGSLPSLKTLDSLFPLVDGLLRTASIAAGVEACRKHASPAIRESLSIQLFIGAVASSGGGISAQTLSVWEPSWRLNRPAFLQEGTLLAGTDVWSGALVAAIYGCLSASHPHYSSLIGLLYSSSFTNSPILSPTDAKATAASILTLIYCWRVYHVHYSKSSVVGTKKAGQKSKSKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9G2</accession>
    <name>A0A5B0P9G2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9G2"/>
    <dbReference type="OrthoDB" id="2499072at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051279">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033">
        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033:SF0">
        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="422" mass="47123" checksum="A8C5B2AB5E562018" modified="2019-11-13" version="1">MASTRSSQAKRKTPNRFQSSISAFNKQGKQPPTTRTKKAAAKSTLSKKKSSKSKKQQTSNSSSQGHRSPSPTQTQGAQNNPDPESSDSTSNSSSSSDSDSGSPLIGFTLDNFQTKLSSWSANQLRKTLQQKKNLSNRVPVDVQEALQLLQQNYLKSKLMLALIGNIGEKTFNKYLGENKPPRKKCGWNRYVAFSLESLKHPVPPKGTSEGWDERNIQMGKAWDLLTDDEKEVFGSRVFQHFSKIPCGYDDEEDDDDTSGQLTPEEIGLYEPLYQLLVNHEKVKAFTANRPESEGQNTGEVYKKALKNVLKLNSELYTTSNMYNTTYYLLSATRAPGINSFCKEYSNDPAWLALAEKRWNSKETFEAYSHGRQIQASLEDIEGGPASSKPLREGDTLKKKLRAALNRLLGELFLCNLHGGNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA08</accession>
    <name>A0A5B0PA08_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029593</name>
        <name evidence="3" type="ORF">PGTUg99_028238</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA08"/>
    <dbReference type="OrthoDB" id="2498113at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474118" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="58" mass="6241" checksum="6209F2DAC692878C" modified="2019-11-13" version="1">MSHKMVAASLVFLVVLIISVTASPAISPSVNTIIKRSYPDAKPLKYVKRSPTANGKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA14</accession>
    <name>A0A5B0PA14_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA14"/>
    <dbReference type="OrthoDB" id="10277267at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022742886" evidence="1">
        <location>
            <begin position="21"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="133" mass="14802" checksum="BA62EA7F19997121" modified="2019-11-13" version="1">MLFSKPLIIVSMIACGFVGATELTKEQKTKCTFTCSNYPHMHDGGCGKHIERDAEGKPIKWQLVKAHRTEKHPGYFNCLGTDAAFSTCCLPHKLPKDGTLTLMNKKNDPQAYDNLCRDSKPDRIAMQLEGCSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAA9</accession>
    <name>A0A5B0PAA9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017749</name>
        <name evidence="3" type="ORF">PGTUg99_019544</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000173">
        <property type="protein sequence ID" value="KAA1126066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAA9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366392" evidence="1">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="67" mass="7213" checksum="FEA75F07055750DE" modified="2019-11-13" version="1">MWILRLYSLILLVGSINCAFGGHHGNPCLASSGISVSLREHCCDSNHTWLMKKAGCGSVPPSVKRMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCL5</accession>
    <name>A0A5B0PCL5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCL5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="271" mass="30758" checksum="066099D2064D2A61" modified="2019-11-13" version="1">MRFPVLYLFQGYISSTDAQKNAYDIALLISSSWSNDRPREPDGSSRNLKRTRSEVIEISSDEDGDPKTSSRSPSNQDRSRDLSSIPPPQAPPNLNQSSKTSPNPSSYPTDQGPKDSDPSKSEPFPNYWNSAIKVTFNDWYPDCPNALRVEDIIGPKDRIKMALVSSYVLELPWIHKLFNPRTRIMVIRHHTDLMTCEHVLVPSPNAQNCNGNAQNRLHAYQVEKSISSNLIFYCRSKLSNGVPILMILRFFIIFYDNFVDRDPTANAVSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIZ4</accession>
    <name>A0A5B0PIZ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIZ4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022839174" evidence="1">
        <location>
            <begin position="19"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="94" mass="11025" checksum="C36C5B86441F020D" modified="2019-11-13" version="1">MQIFLFLLGLLLSREAIAAPAAPVKALVLEHRALLWYIHLQLGRVSSKSDHPLDLLFFNLTSHTQDYLYILTVASLYYYELLAQFWDLGKYYYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKP7</accession>
    <name>A0A5B0PKP7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_010918</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKP7"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02124">
        <property type="entry name" value="PA_PoS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07489">
        <property type="entry name" value="Peptidases_S8_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003882">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000051">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010435">
        <property type="entry name" value="C5a/SBT2-like_Fn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034187">
        <property type="entry name" value="Peptidases_S8_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF66">
        <property type="entry name" value="SERIN ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06280">
        <property type="entry name" value="fn3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 9 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="11">
        <location>
            <begin position="152"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="12">
        <location>
            <begin position="405"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="C5a peptidase/Subtilisin-like protease SBT2-like Fn3-like" evidence="13">
        <location>
            <begin position="627"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1101204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="922" mass="100264" checksum="0F30031A37B2E09D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLF4</accession>
    <name>A0A5B0PLF4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017618</name>
        <name evidence="2" type="ORF">PGTUg99_036429</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000035">
        <property type="protein sequence ID" value="KAA1136622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLF4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1136622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="111" mass="12000" checksum="7C90A952D2F24A8C" modified="2019-11-13" version="1">MARVSFNHPETVLAIEALTNICTLPGCDVTGWIVRAGVSGRNGWVISTVSRFLFRGLFFLDALCTHNSCAASQSPLHSSSIFFFIHLQSSNCLKVQSRLTLADGLPDGLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMI9</accession>
    <name>A0A5B0PMI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PMI9"/>
    <dbReference type="OrthoDB" id="2509965at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1102166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="520" mass="58530" checksum="FEC472D0D9E6B449" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PS08</accession>
    <name>A0A5B0PS08_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PS08"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038921">
        <property type="entry name" value="YOR389W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204:SF1">
        <property type="entry name" value="ENTEROTOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204">
        <property type="entry name" value="YALI0A21131P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="613" mass="69001" checksum="A42609067721D8E6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PV89</accession>
    <name>A0A5B0PV89_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_004597</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PV89"/>
    <dbReference type="OrthoDB" id="440553at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17325">
        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF23">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="18"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1103919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="469" mass="50529" checksum="62AD332E71656963" modified="2019-11-13" version="1">MNKKLPWGLHWRSSKYFILIVVIISLFLDLFLYGLIVPVLPFILKDRLHLPPDRLQISASILLASHAVATFFTAPLIGLSIDGSMSRRHGFLLGLVVTLGATVFLCFGNSLWQLIIARLLQGASAAIVWIVGLAIVTDTVPQDHLGKALGTVYSATTLGALLSPTIGGLVYTKFGYYPVYYLAGALLSVDIVLRIIVIDKEEAITFADCAAAGPQLAPMETGERSLLLGPAETCRPDEPPRLMQHDEEERSGSKSGAVRFGSPKHCCPIITILPDPRLLAATFVTFTHACLVGMFNATLPIHVQKVFGMSSLESGLLFIAIELPYVFCGPAIGWYVDYAGPRGPASLGSLLLVPSIFLLRFPQIMQDLPFLILLLALSGTGLSAASSSAFVQANKIGRERHVENPILFGSKGPYGQLYALNNILYSLGMTVGPLVAGALSNCFGYGNAMAVMALQALLAAIISRTWMER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PVD4</accession>
    <name>A0A5B0PVD4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_030308</name>
        <name evidence="3" type="ORF">PGTUg99_010602</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000414">
        <property type="protein sequence ID" value="KAA1086057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PVD4"/>
    <dbReference type="OrthoDB" id="2500434at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="594"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="652"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="681"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="721"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="768"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="808"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="822" mass="91634" checksum="97AC55EA0404221E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZF2</accession>
    <name>A0A5B0PZF2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pinin/SDK/MemA protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_032832</name>
        <name evidence="11" type="ORF">PGTUg99_030171</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pinin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZF2"/>
    <dbReference type="OrthoDB" id="330772at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039853">
        <property type="entry name" value="Pinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006786">
        <property type="entry name" value="Pinin_SDK_MemA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12707:SF0">
        <property type="entry name" value="PININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12707">
        <property type="entry name" value="PINN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04696">
        <property type="entry name" value="Pinin_SDK_memA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Pinin/SDK/MemA protein" evidence="9">
        <location>
            <begin position="71"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="245"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1106302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1109352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="321" mass="36331" checksum="E144F0816C226A1F" modified="2019-11-13" version="1">MDTHSQPAQATEDSAGPEDEIRAAEAEFASKQGGTPSDRMVDESPAGEHPTGRPLDSADQQPRKKLKLTEADKKRNVRMFGSLMGTLSKFQDETSKIKQTDAAKRRAAVESRLQAKLKLEAESLHLLRACEVEEANLKQQVLRKADELGHLSELHTIKFANKLAFSHYLRTSTSKLTDADQNEPPLTTYGRPRHSEQPIFWLPHKLLPEQKELIETQRREVQEEVEEQRHQWEEERLVKEEELEKMQAHRDTRLAEIQAEKAQARANASTDPTPDPNSATASLNVTANETAEVARRQTSEMRQDTPDVVIDNGRDEDAVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q3F7</accession>
    <name>A0A5B0Q3F7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_021151</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FRG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q3F7"/>
    <dbReference type="OrthoDB" id="5539371at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23339">
        <property type="entry name" value="beta-trefoil_FSCN_fungal_FRG1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010414">
        <property type="entry name" value="FRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928">
        <property type="entry name" value="FRG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928:SF0">
        <property type="entry name" value="FSHD REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06229">
        <property type="entry name" value="FRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="281"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1107658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="331" mass="36099" checksum="108F505CB3070669" modified="2019-11-13" version="1">MSSSKSSSKLMFKGEKKEKKKHRKRRRDEKESNEDGPGSSEPSEGWLNPPSQAHILGPLYMICVQPTYGNVLETKPCAIGIQPSPVTPIAKVVPAPLSASALASLEPDEVNHVMVCTRVIDSDDKVTLRTATGRYLAADQLGAVSAEQEARGAQEEWAFEPLALPEDQPSGSTTNPDGHQSPPDGLFALKSCLYNKYLTVEELASGKLDIRCDSDSPTDDCSHILVRMQAGELVKAKKRLADERAKKGLSAKSNESGLTILKPGQTFHDLEANNIRQYQARGAGRLQLPSESKSGLKKARKEGRLAEELLDRRSKLKSDRYAKVQSPPAPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q550</accession>
    <name>A0A5B0Q550_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q550"/>
    <dbReference type="OrthoDB" id="10299334at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="94" mass="10423" checksum="59716F6906C8EA0B" modified="2019-11-13" version="1">MTLGSVDAIVTPCQQIGGCTRYFNDHRLLELPVGRCNTQYTCTQGHTHLCTQNREQWHTPGTCGHVRVGTRCTAPHQHHHNPHAQGYTPCPGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5V5</accession>
    <name>A0A5B0Q5V5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014484</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5V5"/>
    <dbReference type="OrthoDB" id="10634002at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023129401" evidence="2">
        <location>
            <begin position="20"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="411" mass="41106" checksum="FED29B058061C070" modified="2019-11-13" version="1">MMIKAAWIIVAALFIGKSAELAIPAKSDASSYGQYKPDVPQNPSPSWPGKPGSGDSDTENGTTKDADSKAGSGADSPPTPTGPTSGLEPPSTHMNGTIADADSQPAPDGASPAASPTGPKGAFEPPSSQMNGTASDGDSQPAPISGVDSSPEQKNPNGSYQSPSNGMNGTIADAQGKPGSGPDSIGSPSQNGTLSSPVDKQPGGDQPGGNQSGDNQPSAGQPAGGDQSSGDASGPGHPYVKRNPASTVSPYPAGKNSSDCDQPGPSPYQGSLNNGTQPALDDKDASSTTAPDEQPDHSGDKKPLPDHTPGSEEGDEDSDDEGSDEDEDENEDEEQDGEADGSKKKKGQSKDASEGSADDGPSSVPPQTPANGEVQSPTSKKPAADDHSDSYRSLASPADKHKNPAAPGAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q734</accession>
    <name>A0A5B0Q734_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 62</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">VPS62_10</name>
        <name evidence="2" type="ORF">PGT21_029704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q734"/>
    <dbReference type="OrthoDB" id="2498178at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48203">
        <property type="entry name" value="BNAC01G40110D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48203:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="211" mass="22826" checksum="1FD495FB3D0C44FC" modified="2019-11-13" version="1">MTAPHSLPESRVVNPVTSQPAILTTTNTFRGENGGGVGGPGSRTLSGEGGTDPFVRNSLGSVPAYVLEYAPMVHLNSNEPFWPASLEEHLRHTILHDHAPDGPATRPADHHHPCSILALPQFNHERCYLTANTDVKPAPTDHPWLVSLEGKPGPDAKSQVSPVILILVDKSIITGVPGTLDAFWFFFYSFNLGPTVANIHFGNHIVRLPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC59</accession>
    <name>A0A5B0QC59_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Trifunctional dihydropteroate synthetase, variant 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">FOL1_2</name>
        <name evidence="16" type="ORF">PGT21_025685</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the C-terminal section; belongs to the DHPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QC59"/>
    <dbReference type="OrthoDB" id="615426at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="entry name" value="7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="15">
        <location>
            <begin position="227"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1110564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="521" mass="56554" checksum="A0F3A93E143A2A78" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCA0</accession>
    <name>A0A5B0QCA0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCA0"/>
    <dbReference type="OrthoDB" id="2507454at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="96" mass="10533" checksum="8B84BFBEA2FCE1CA" modified="2019-11-13" version="1">MSTDRGSNPTKDAPAHPTLDIKPTTMSTPSPKISTARLPILEAPGPQSNYLDWKLVVNHEVFKSQKSVTYSPLRSQQIVPRLGKTMTTLSVQSSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDA0</accession>
    <name>A0A5B0QDA0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="533" mass="58834" checksum="4A74E4F24FD06CF6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFE7</accession>
    <name>A0A5B0QFE7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFE7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="208" mass="23572" checksum="2738D16CD43A99D4" modified="2019-11-13" version="1">MAAIHSLVLRPRPASIVLRRVRLKFLSKRPSCVQRLAALLYFSCGEVLCIDLFVIVHPTQTQKQASPTTSLINRKLLDSADLSSVTFPASLIINTACGLVTSEVINFYTNEIRSFGCDPSEVALCIVTRYTRPNDRNSRPDYYLLDTAVRVKPGTYNCGVTTAKCCGTNLKYEKWQHVKDSQWMKDLTETFCPNKVYYVDPKKPRTRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHB0</accession>
    <name>A0A5B0QHB0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHB0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="248" mass="27155" checksum="2632C6A66753F581" modified="2019-11-13" version="1">MPGSYGIALVLNPYSYGFRTCTPIYAKNLAADWRAGLHANLLWRVGLHANLQVACRLACALGVGVRLSTPVWEATSYPLFLLRKCQKQAALATLGSPALASKSEGQNFKNNYKSLVPQQSAGHHSLADNYTPPAPTPPSTRSRTSPARVTHRPTSRFPTAPVLVDPKSSTKASLRYIAHRTTRFLFKQLDPFPFKRLDPFPFKRLAWDLLGSFSDEDGEQLEGSDQAKIAAALADCGAKTAPIIPFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QI27</accession>
    <name>A0A5B0QI27_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QI27"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="114" mass="12375" checksum="042A915F944BE241" modified="2019-11-13" version="1">MNSTANPAWLRVQPSSAPTLPLARKSRRTPIKTDGPVVYLGQSSDDPNLSQSHRIPLLAASLDEVSMDQPAGTNTHKIGLLLSTSLVRSQPPPSSSPMRPVPAHNKLLDFPYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QI69</accession>
    <name>A0A5B0QI69_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_005865</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKN1/KRE6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QI69"/>
    <dbReference type="OMA" id="TAPFDDY"/>
    <dbReference type="OrthoDB" id="412647at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02180">
        <property type="entry name" value="GH16_fungal_KRE6_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000140">
        <property type="entry name" value="Beta-glucan synthesis-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000135">
        <property type="entry name" value="Related to KRE6-glucan synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005629">
        <property type="entry name" value="Skn1/Kre6/Sbg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361:SF15">
        <property type="entry name" value="GH16 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03935">
        <property type="entry name" value="SKN1_KRE6_Sbg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="11">
        <location>
            <begin position="214"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1112674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="623" mass="67256" checksum="4C51DFB6298571C9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJQ2</accession>
    <name>A0A5B0QJQ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJQ2"/>
    <dbReference type="OrthoDB" id="10442696at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="151" mass="16907" checksum="9306A9CC909B5DA5" modified="2019-11-13" version="1">MNVGRKWCILDPVDPTIKPSVSRLKLGLEWCYIQQGQAGIVLKANIPPTPESAAAVGSLHYWDPKDDTRYDTFLFSSPPNLLLEYKNKLDDWLLVDSSEALSKQFTLPAPLFDDKVQHTSSDLPGAFPVDQVVSQVDYYPTIQFLPGKVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QKL3</accession>
    <name>A0A5B0QKL3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021032</name>
        <name evidence="2" type="ORF">PGT21_022080</name>
        <name evidence="3" type="ORF">PGTUg99_022445</name>
        <name evidence="4" type="ORF">PGTUg99_031167</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000276">
        <property type="protein sequence ID" value="KAA1113700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QKL3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="56" mass="6160" checksum="20D2DB44EE6A1C6F" modified="2019-11-13" version="1">MRKAGNILGAAAMSHIQLLSLLSQYSENPTIFDRPGQRRGVLFVDNPWRGCNSSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMD4</accession>
    <name>A0A5B0QMD4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMD4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="59" mass="6591" checksum="B82F86524CF6409B" modified="2019-11-13" version="1">MTTSIHKLHQSTDQNLPKAIGQHYLELDSNGMSESSRAERVMHTQIGAPTSGVRVKRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMH8</accession>
    <name>A0A5B0QMH8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMH8"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="48"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="134" mass="14547" checksum="BEC8BE59032247D0" modified="2019-11-13" version="1">MAYKRHKKAGYAKRPGFNRHASFIMSSPPIIYEDEASYCTRYTDTTTRLVKVSNLTLGTSEDNVRAAFQEFGNINHCFILMSAPTAAWLVFATPAKALASIVGMKGAVADGRKLRVHGIPSASLGGLALLLRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ57</accession>
    <name>A0A5B0QQ57_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sphingosine N-acyltransferase lac1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LAC1_7</name>
        <name evidence="9" type="ORF">PGT21_035365</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ57"/>
    <dbReference type="OrthoDB" id="2503026at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13857">
        <property type="entry name" value="CuRO_1_Diphenol_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13883">
        <property type="entry name" value="CuRO_2_Diphenol_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13904">
        <property type="entry name" value="CuRO_3_Diphenol_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF414">
        <property type="entry name" value="ADR239WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022882654" evidence="5">
        <location>
            <begin position="26"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="69"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="197"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="573"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="461"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1115338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="706" mass="78550" checksum="CA3EF74CCAA3725B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTZ8</accession>
    <name>A0A5B0QTZ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTZ8"/>
    <dbReference type="OrthoDB" id="2518924at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="622" mass="68754" checksum="DAB94EF7019F086F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QU19</accession>
    <name>A0A5B0QU19_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_012934</name>
        <name evidence="15" type="ORF">PGTUg99_019249</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="15 18">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000474">
        <property type="protein sequence ID" value="KAA1073897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QU19"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45977:SF4">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45977">
        <property type="entry name" value="TARGET OF ERK KINASE MPK-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="233"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1073897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1116403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="284" mass="32128" checksum="D84A820979E2F848" modified="2019-11-13" version="1">MTQRSNPSELDGNLPGEEGTSGPSSTTPEMSPPVHNSAANHHASSDNIYGPEDEYLIFGSSGIELTNDESEAEISEHPPPAVGFEALRKDPKYSAYHQIPPITRCLAVLRPLEEYLEKLKPRDQTKIARYFRQLDRQMSEMLHGPLQPRDFIFLHETSTTAIDRSISKLFALNMETITQIVQTMFHDRVDLSLHSPHSRHRPQAVDWLLDSLGLTPVSSLLSEDPDPDRIERCSLCLMEYNPEIPVTVLLCHPEHHFHPDCIRSALNKVARCPICHIDVLYPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWH8</accession>
    <name>A0A5B0QWH8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019439</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWH8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7914" checksum="25F1833E610087A2" modified="2019-11-13" version="1">MTVMAKLSVFLEEAGAEEIDAITITHALKRDADKSHETTSVRGMQVTRLKKMLIMACLKVINHLFERWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWU6</accession>
    <name>A0A5B0QWU6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWU6"/>
    <dbReference type="OrthoDB" id="2508462at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="157" mass="17699" checksum="D01A23977C6CB2C3" modified="2019-11-13" version="1">MADSMNPKPVALSFEPIREPLKVLLAQQNIGRISSLQVDVLFEARRLSHPLSFTQVTNNLTVNLIRDLMGHLSKEIPLLFNSQWQKFLTKKLATVIDMSKYTASIAASAKDKEARHQVAKMPVTIDMSQSDQQLVIDSQDKIANQLFKVLLHHGRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R393</accession>
    <name>A0A5B0R393_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R393"/>
    <dbReference type="OrthoDB" id="26149at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040144">
        <property type="entry name" value="RAP1GDS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10957">
        <property type="entry name" value="RAP1 GTPASE-GDP DISSOCIATION STIMULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="655"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="752"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="655"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="796" mass="89126" checksum="56FEC86BD6A24B85" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3S8</accession>
    <name>A0A5B0R3S8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_037205</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07543">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000056">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000058">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000071">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047820">
        <property type="entry name" value="P5A-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="424"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1064"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1142"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1182"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="15">
        <location>
            <begin position="23"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="14">
        <location>
            <begin position="275"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1225"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1232"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1272"/>
            <end position="1281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1120148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1281" mass="142466" checksum="42A45CEBCD490719" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RBH0</accession>
    <name>A0A5B0RBH0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">CDP-diacylglycerol-inositol 3-phosphatidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PIS1_2</name>
        <name evidence="10" type="ORF">PGT21_004981</name>
        <name evidence="11" type="ORF">PGTUg99_018687</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000211">
        <property type="protein sequence ID" value="KAA1123171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RBH0"/>
    <dbReference type="OrthoDB" id="10251079at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003881">
        <property type="term" value="F:CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1071356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1123171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="266" mass="29356" checksum="76A413A8C2737DC7" modified="2019-11-13" version="1">MTTTTNQPTSSSSSSSSQTRTSTQPLHHQSNIPSTSDENVFLFLPNLIGYTRIILAAVSLHFMSYHPIYCTIAYIISQLLDAVDGQVARMLGQTSKFGAVLDMVTDRCTTSCLLCFLSSVYPQWAIMFQSLIALDFASHYIHMYSSMVAGSKSHKTVSKKQSRILHSYYTNSGNRGRQDALRSRWLPPKDHPTTCRSRNGDHSGFRKKHPTHSFLVDIGAPEAYEGNAGQAWSTASRPHWYTSILPIAQDSAAIDTCLKSLPSGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RE28</accession>
    <name>A0A5B0RE28_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_022367</name>
        <name evidence="7" type="ORF">PGTUg99_025443</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RE28"/>
    <dbReference type="OrthoDB" id="79252at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053051">
        <property type="entry name" value="HDAC_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="5">
        <location>
            <begin position="136"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="296"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="536"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="376"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="424"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="552"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1082998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1124071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="601" mass="66439" checksum="F7EE9ED1E3026228" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RE36</accession>
    <name>A0A5B0RE36_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022085</name>
        <name evidence="3" type="ORF">PGTUg99_026121</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RE36"/>
    <dbReference type="OrthoDB" id="10590621at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="135" mass="15077" checksum="D8DA8045F468A393" modified="2019-11-13" version="1">MRGIQWSNSHIFNLKNGGVVLKGTGSCWKSNSRDRGSTPKRTRTQQIANAAVFDQEQHTNVTEPGTEPDNSTNPNRSSLLDRLSSPIASAEFNKNSLSEQIDKPPATDTYNNQNSRPYPERNKEASAYWLAQQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RHP5</accession>
    <name>A0A5B0RHP5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003514</name>
        <name evidence="3" type="ORF">PGTUg99_032531</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000204">
        <property type="protein sequence ID" value="KAA1124435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RHP5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="62" mass="6237" checksum="4DA849FC9485897F" modified="2019-11-13" version="1">MAGEGPWPANLTQVHRLPRQATSRQLCETGAALRALTREALLSSPGPGPGAPSGMGGVGSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RI38</accession>
    <name>A0A5B0RI38_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chorismate mutase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_033810</name>
        <name evidence="5" type="ORF">PGT21_034177</name>
        <name evidence="6" type="ORF">PGTUg99_010712</name>
        <name evidence="7" type="ORF">PGTUg99_023567</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000176">
        <property type="protein sequence ID" value="KAA1125611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000070">
        <property type="protein sequence ID" value="KAA1133832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RI38"/>
    <dbReference type="OMA" id="WVANATW"/>
    <dbReference type="OrthoDB" id="2843337at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009697">
        <property type="term" value="P:salicylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051331">
        <property type="entry name" value="Chorismate_mutase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38041">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38041:SF1">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033845310" description="Chorismate mutase domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate mutase" evidence="3">
        <location>
            <begin position="97"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1098342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1101955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1125611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1133832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="201" mass="21991" checksum="2C60890BCB53136C" modified="2019-11-13" version="1">MLALRLCLFSCLAWLAHATPKLDSVPGPTTLSLPKSQHPNGVCYQSTQAGYGPTVDPHGNAVKYGSDEDISYGRDPKKHVPGTAVWAQPTITLSNGTKCCDSLTQVREYIDYLDKTILDYLAIRQQFVVEAGRFKDSKTAVRSGIRAVQVVKNAQTAAEKNGLAPWIANATWTTTLNSFTALELCLFDQDVKTKNYAPDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RIQ9</accession>
    <name>A0A5B0RIQ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034648</name>
        <name evidence="2" type="ORF">PGTUg99_019523</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000176">
        <property type="protein sequence ID" value="KAA1125670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RIQ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1125670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="114" mass="13180" checksum="A3F478645D9DC61C" modified="2019-11-13" version="1">MSNDSIVPNLTYSETKISYKKNTDYIYSYFWKTKIIKTYQITTHSTYLNSTKELLGENKHKRLISVITKKTRENLDSGESSTINPSIRENIAETALEPELPPLPEDFLNSHHVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RN72</accession>
    <name>A0A5B0RN72_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GCD7_3</name>
        <name evidence="11" type="synonym">GCD7_2</name>
        <name evidence="11" type="ORF">PGT21_016857</name>
        <name evidence="12" type="ORF">PGTUg99_030277</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RN72"/>
    <dbReference type="OrthoDB" id="269919at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051855">
        <property type="entry name" value="eIF2B_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1076724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1126889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="423" mass="45997" checksum="D6843F8332542DB5" modified="2019-11-13" version="1">MAIKRFTHEISKAQILKRIESFGYKLQNRQIVGSHATGSETIKLLREVIASAKFSSFDQLCGHLVEVGDFLSEIAPREFVIGNMVKRVIILLKEEYASALASHIEGNSERLKTSKVGSDTHHRTTTSSSHCSTSRSPGIVEESVSRLLGQKPMTNELLEEDEKIPADLINEERAFNANSLSLKPIFIEAVQELYDELDLVLEAVAAQASEHIHSGEVIMTLDNSRTVTSFLQAAAKNKRKFTVIISETGPEFRGRELSGKLTSSSPKIPNIVTSDGSTFGLISRCTKLIIGCHIVFADGSILAKSGSLGLALAAKVHNVPVVVISGIFKFAPVYLSAGDQWTTLDIKTPDQVLAIDQILPDPPPAASKSNLASIHPSPTPGQIEVINPYYDHVPAHLISLFITNLGGHPSSLVYRLLKDLYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RQ25</accession>
    <name>A0A5B0RQ25_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_001645</name>
        <name evidence="6" type="ORF">PGT21_001797</name>
        <name evidence="8" type="ORF">PGTUg99_010917</name>
        <name evidence="9" type="ORF">PGTUg99_021461</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calmodulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000145">
        <property type="protein sequence ID" value="KAA1128021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000141">
        <property type="protein sequence ID" value="KAA1128529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RQ25"/>
    <dbReference type="SMR" id="A0A5B0RQ25"/>
    <dbReference type="OMA" id="ARKMKEC"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000006">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000398">
        <property type="entry name" value="Calmodulin-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1064405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1085281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1128021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1128529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="149" mass="16737" checksum="C8E166B0041CE1DC" modified="2019-11-13" version="1">MADQLTEEQISEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELGDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFKVFDKDGNGFISAAELRHVMTNLGEKLSDQEVEEMIREADVDGDGAINYEEFVRMMLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RV32</accession>
    <name>A0A5B0RV32_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_021789</name>
        <name evidence="11" type="ORF">PGT21_022668</name>
        <name evidence="12" type="ORF">PGTUg99_031342</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ATP = O-phospho-L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23052"/>
            <dbReference type="Rhea" id="RHEA-COMP:13689"/>
            <dbReference type="Rhea" id="RHEA-COMP:13690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="8"/>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RV32"/>
    <dbReference type="OrthoDB" id="241648at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16929">
        <property type="entry name" value="HATPase_PDK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF3">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="304"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1094450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1108715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1129139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="440" mass="49358" checksum="97ED08639BB2E175" modified="2019-11-13" version="1">MAGWKLTQALKDKISHFASFPQTGVSLRQMVMFGQNPSAGTLMRASEFLHEELPIRLAHRVKELNELPNRLNEMPSILKVRDWYAQSFEELVNFPRPELPPEIIAHFEKAFTKGAPLPALTPNPSLPPHMQRPLLDTTGKQRLGLSQRYYVDLGDVVWPPEVHDYNERFTQTLSVIKRRHDPVVTTVAQGILEYKDFLAGPGRSVGTKASASPSVIDRSVQTFLDRFYMSRIGIRVLIGQHIALNKLEPHPDYVGVICTNTNVYQICREAIDNALFICEEHYGLFKGPPVQLVCPKDLAFMYVPSHLNHMVFEVLKNSLRAVVETHGVDADEFPPIKVIVAQGAEDITIKISDEGGGIPRSAVGLVWTYMYTTAQAQRLDQDFEGTDFKAPMAGFGYGLSIARLYAQYFGGNLKLISMEGYGTDVYLHLSRLSTTTEPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S2K5</accession>
    <name>A0A5B0S2K5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DPH5_1</name>
        <name evidence="13" type="synonym">DPH5_2</name>
        <name evidence="13" type="ORF">PGT21_029401</name>
        <name evidence="14" type="ORF">PGTUg99_028213</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S2K5"/>
    <dbReference type="OrthoDB" id="2516at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1081101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1131263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="312" mass="35178" checksum="7C466B7F9EA7B41F" modified="2019-11-13" version="1">MFYLIGIGMSSPEDITLSGLKTIQDSKKVYLEGYTSILIDSQLEDLQALYGKDIILADRDFIETRSDEILDQAAEEDVSLLVVGDPFGATTHADLLLRCTKKGIKYRVIHNVSILNAIGSIGINLYHFGQTVSIPFFNSNWRPKSWFKKINQNFNLGLHTLCLLDIKVKEQSDENLARGRKIYEKPRYMTITTAIEQIISIIEEFKQEDKDSEEEDSEERYTNRLTDPAQILCIAACRVTSKTEKFLVGSMAELAKLEPEEFGGPLHSLIILGDDPSKKNLNPVEIEFLSGFAVDKQSWFRLTGPSSNQLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S2K6</accession>
    <name>A0A5B0S2K6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023788</name>
        <name evidence="4" type="ORF">PGTUg99_007507</name>
        <name evidence="2" type="ORF">PGTUg99_020390</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000081">
        <property type="protein sequence ID" value="KAA1132391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S2K6"/>
    <dbReference type="OrthoDB" id="2503827at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1132391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19167" checksum="349AD5DCA202B0DB" modified="2019-11-13" version="1">MMNAERREEERMLLEPRLGSLTLDGETGERLDQSMAISMVDQLKQLLINSLSSKGSAESRLEISNQANLLKQTATKLERQIKVLTAGDLSLLDQEELISSLESRIQIEREILQFMSDTLPSKKETATTTGPTPSGNAATDTSSSEQVQTDNLLETFNQTSQKDGKQEDTGMEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S6C0</accession>
    <name>A0A5B0S6C0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027444</name>
        <name evidence="3" type="ORF">PGTUg99_013704</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1132965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S6C0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366674" evidence="1">
        <location>
            <begin position="22"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="101" mass="11018" checksum="8985060B6D583401" modified="2019-11-13" version="1">MQINVFASLGLALGLLGSAAAELLFACDPIVALQPLGLCVSSDGIPIRAEKYRNSDRIFWPCHGQRREYCCPASEKLYVRLGASIDPETLANDCSRQVPHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S717</accession>
    <name>A0A5B0S717_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial zinc maintenance protein 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003566</name>
        <name evidence="3" type="ORF">PGTUg99_030231</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S717"/>
    <dbReference type="OrthoDB" id="2501943at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20610" checksum="19F486CA2F025096" modified="2019-11-13" version="1">MAGRLAFSHAQKAQAASAYRNLIRSSKFTFDSDPQTYRQFLVRAKELFSTKVLNPTYLLTSPADPKVAASASNVPEEVVMRERFEHELRGVLELSKYLTRNIVQGKHSEDGRRLVLRFTDKTEVGDNSTIKNPGSTASCLPTSGCCGGSHDSPSTTPEPPPSRLSQAALERREKRRLNRLQQSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LIU7</accession>
    <name>A0A5B0LIU7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LIU7"/>
    <dbReference type="OrthoDB" id="2495484at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1064767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="454" mass="52033" checksum="88BBB086C401DFE2" modified="2019-11-13" version="1">MIMDSSTISEETKGLIVKALEGLANESWKTHDGEFPTFPEETFLSHMIDTGKADLIKLQTISLPALRQQLLGLIESFDLRTLDKEHKPKYPDVLEITHQLGPTLNLIDTSTESISPAIGSEAMELLSKVDHDYGIVKRYRCEELRARLHVLMTHNLRNLFGSYWEFVQSWNFVGPASSDSDHDLDMIRLRDSIIELTDESCQVIDDDIIKWSKRSDFAILQKTWQEQADDFSDTLNFMAQQIDYNVNFKKKPKEGAPRNNPRENIWGGYNGFDAPEDQAETDSEDDGSLPEFNLLNLELIPSIRSTIPLLKLGRIFFTKLLDTPKGKPPFSLSDRLSSYELEELKTTTISFRICIMQILHAIYEAEDLAGLNSQRVDIMPLFDQGPPRALDKSLTLLAFFLVPKPSQLDLPLPENPFKEWFCEFRFTFSTALQQLRENLFTSDSERTYSPDEMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJS0</accession>
    <name>A0A5B0LJS0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014513</name>
        <name evidence="2" type="ORF">PGTUg99_007595</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000515">
        <property type="protein sequence ID" value="KAA1064130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJS0"/>
    <dbReference type="OrthoDB" id="2495444at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="765" mass="86950" checksum="A2CD6E5B7E547080" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LK48</accession>
    <name>A0A5B0LK48_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012811</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LK48"/>
    <dbReference type="OrthoDB" id="10610746at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1064731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="158" mass="18005" checksum="0C8CAD0169D5B786" modified="2019-11-13" version="1">MSTLYAIQLQDAQKTIHWLEDEASKLRKGVNIQLIRLQEENSRLHQQLSDAQNKLQQAHRELNDRANENSTLRNRVKNLQLRIDLLTQQMMHCAQQQPGMFQPAWSRGTMPHQSNEGHAMGMPSMAGMTQNYHPHTLPNPMTGSSMSGDSLNIPPQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQX7</accession>
    <name>A0A5B0LQX7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQX7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="11476" checksum="F79A714D38D576A2" modified="2019-11-13" version="1">MGLGEQEASTEDSRAKATITLLTNAPPFVLSFVRSSIEPTYPRSRSEENQPDYTRIVRPIVDLIQIRIRLRSLGTRPNCLPKERKSSLLPSLDSDLHFRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LR32</accession>
    <name>A0A5B0LR32_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Uracil catabolism protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013475</name>
        <name evidence="1" type="ORF">PGTUg99_007014</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1066892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LR32"/>
    <dbReference type="OrthoDB" id="2153176at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012469">
        <property type="entry name" value="DUF1688"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31687">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31687:SF3">
        <property type="entry name" value="PROTEIN URG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07958">
        <property type="entry name" value="DUF1688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="59878" checksum="65F411AD96BDA1EF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRX1</accession>
    <name>A0A5B0LRX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013966</name>
        <name evidence="1" type="ORF">PGTUg99_002524</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1066876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRX1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1066876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="55" mass="6349" checksum="DF005FEBBDD8362A" modified="2019-11-13" version="1">MDDYTVATAAWDAEPPFGPDLPISITRYDLFYWLAHSALDSYSPHCQRRAFRQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXA2</accession>
    <name>A0A5B0LXA2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXA2"/>
    <dbReference type="OMA" id="IWNSHIN"/>
    <dbReference type="OrthoDB" id="2498221at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="505" mass="55769" checksum="268547D0AF65835D" modified="2019-11-13" version="1">MSDKMDIDDQGLQGAVDPKATPPGDPAKSTGGYSWTYAFEVPLLTLELVCEESEKKTIKQVESELGVTIEFERTTPHYKGFGNGLCRILARETLAIYRALALIVALVRLSSPARYCFWTHWLVLDEVFQIDSIPDQTDRVETGVEHVETKSVDDGSGDGHQDSSTSLANDSTQTISGTLGEVHNVKTTASSQSSEPPKNSGWAIESNKDSQSQIPQSGWEDPPAPCLPLAPSEPQIPLSTTPPILEPSGSATGPPLETINQDYFGSTTPCSSPGEVAQTNVDSERLPTQPLTPFQEELLSDKLISDQDYKRMLDVDKAQLPQDTQAHHPSFEWLQEAFKLHNPGKPGPHQVDLGKDAQLNRSSTSTTPLSELAVKKSEPPIETIEAPMKKSEPPFETTEPPVKNSELRVEKSEPPIKKSEPRVEYSEPHPIENKAVIKRLMELPLGTEEYLRENNFQAIREIILESNAHCRLSSTPECLRFWAVGTEREVSLAMVLISIKIKEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LY88</accession>
    <name>A0A5B0LY88_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LY88"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="11021" checksum="C91DCED9E7B42596" modified="2019-11-13" version="1">MAQTTVSDPASLTQSLISNKKPSKSSSQPKPITSALHSISQRSAGSNTSQVTTSVSTKDTIGLEEFSKFPKHTRLYKIVKDKFQGLCLPTNKLLESQYWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYJ4</accession>
    <name>A0A5B0LYJ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYJ4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="126" mass="14651" checksum="EC66AEA9A1A54ABC" modified="2019-11-13" version="1">MESVLVLQGFWKSASELSQEKHPAGYNLWKNTPKLEITNMSEGVITFKHQQNHPSKAIFEWHRLDTPILYNESEHPIQFLIQHVKTKAFVYWPRVSEGHGQRVEIPKKYQSSGPFMLYIAESPSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYL4</accession>
    <name>A0A5B0LYL4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Extragenic suppressor of kinetochore protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_026561</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYL4"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="99"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="346"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="466"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="591"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="868"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1142"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="100"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="645"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="669"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="868"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="916"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="965"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="983"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1020"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1048"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1149"/>
            <end position="1171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1069501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1171" mass="126904" checksum="09E7A39BB3E0D369" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0C1</accession>
    <name>A0A5B0M0C1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein mago nashi</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_026742</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mago nashi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0C1"/>
    <dbReference type="OrthoDB" id="6495301at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11295">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1560.10:FF:000001">
        <property type="entry name" value="Protein mago nashi homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1560.10">
        <property type="entry name" value="Mago nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004023">
        <property type="entry name" value="Mago_nashi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036605">
        <property type="entry name" value="Mago_nashi_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638:SF0">
        <property type="entry name" value="MAGO HOMOLOG, EXON JUNCTION COMPLEX SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638">
        <property type="entry name" value="PROTEIN MAGO NASHI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02792">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89817">
        <property type="entry name" value="Mago nashi protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1069510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="31963" checksum="AD74CD21F102E4A2" modified="2019-11-13" version="1">MTNPSTALAVPMSSFPRVWGGMPHETQIPSLSAPNRCSPSAPRRESLVLRPFGDAADRSPLQVHKSVVAEHVPTARVPWGPPLLVDRYLAQVLSTDQNLAPYPDAAQLEHQSVPLSHSRSGKALSTRKKQLETMPAKTDPFYVRYYSGHMGRYGHEFLEFEFSEGRLRYANNSNYRNDSLIRKEMWLSPLTISELKRIVAESEIIKEDDHNWPKKNVVGKQELEVRLSDTHISFETAKIGSLVDVQGSDDPEGLRVFYYLIQDLRIFVFSLISAHFKIKPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3N1</accession>
    <name>A0A5B0M3N1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018811</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3N1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="191" mass="21384" checksum="B6050DFF798D21B0" modified="2019-11-13" version="1">MHSPDFWLWPDAPSSQMSEHQVDDPWVMLFHPRFQWEVGPAPILTVIPSLFQDLTTSVDHHQSCSSTTVHQRLWTLIPRCLSSTIGLFRSSNAPTDYRLFSFLSSIISPTDDCRATSTNSFFCFCFFQRQPGDGSKLGLIVGASTRTTSRFWRGIVQITSRTDPREAVKAVVSIGIELATVDSRATSTNPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6B5</accession>
    <name>A0A5B0M6B5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGT21_025542</name>
        <name evidence="21" type="ORF">PGTUg99_019488</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
            <strain evidence="21 23">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38959"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.3.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; peroxisomal fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6B5"/>
    <dbReference type="OrthoDB" id="538336at2759"/>
    <dbReference type="UniPathway" id="UPA00661"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000006">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000013">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000015">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000003">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029320">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF250">
        <property type="entry name" value="PEROXISOMAL ACYL-COENZYME A OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14749">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 14" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-coenzyme A oxidase N-terminal" evidence="18">
        <location>
            <begin position="80"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="17">
        <location>
            <begin position="196"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="19">
        <location>
            <begin position="337"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="16">
        <location>
            <begin position="551"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1066222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1072697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="733" mass="82498" checksum="A8C576DB3B40744E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MC39</accession>
    <name>A0A5B0MC39_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004673</name>
        <name evidence="1" type="ORF">PGTUg99_031686</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MC39"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="61" mass="6908" checksum="54310FEB8FD4BB0F" modified="2019-11-13" version="1">MNAALQAAWMQRAGSDWLPEDTVGGVALWFPRHERHITVMTSSLGWLSSSLKRRVCHDQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCB2</accession>
    <name>A0A5B0MCB2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Superoxide dismutase copper/zinc binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_004026</name>
        <name evidence="3" type="ORF">PGTUg99_032375</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCB2"/>
    <dbReference type="OrthoDB" id="2495266at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000015">
        <property type="entry name" value="Copper/zinc superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053257">
        <property type="entry name" value="Cu-only_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20910">
        <property type="entry name" value="AGAP001623-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20910:SF1">
        <property type="entry name" value="SUPEROXIDE DISMUTASE COPPER_ZINC BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033473510" description="Superoxide dismutase copper/zinc binding domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="2">
        <location>
            <begin position="72"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="202" mass="21685" checksum="64F861EC594E75B7" modified="2019-11-13" version="1">MSTKMYLFMLVVSTITLTSILAADNPPPQKNPYPQAASAKINNGAGVQVTIDFAKVPKENRPPETSHFKADRPLTSVTVKYAGLEAGKDYTYFIHEKPVTGTDCSTAGDRLDPRKVNTTKDPNFHCDRHRPSRCEVGDLSGKHGVLKGNGQAETPLPVYYDANLALTYSRRGILGKSIVIVDPTGTAVACGNVVDPNAPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEE7</accession>
    <name>A0A5B0MEE7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEE7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="106" mass="11721" checksum="98717B45F5C2F5B1" modified="2019-11-13" version="1">MPGVYSDVLWMPSWAIPGPGTSQVQWHPWAAPIFAKCDVPGTAFCPRDISDRSPSRLPLYPPLRSSKQNKLMPLLRSSVPHGGAGMHYPLDPIPALVPEPRRNRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIV0</accession>
    <name>A0A5B0MIV0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_050249</name>
        <name evidence="3" type="ORF">PGTUg99_050006</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIV0"/>
    <dbReference type="OrthoDB" id="76487at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="290"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1091538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="46514" checksum="D2CCEC4086208BD0" modified="2019-11-13" version="1">MGNNLEQGVSQSKDDARATNTIGSSSTTPELHGQQKIQNIKTGDSNKKKRSPNWLPQEEEQLACSWLVVSQRPEFITNQTSESFFRAVELDFNQHSKLHHRDHDQIRIRWAALNTSTLKFSAIYNALEKSPPKDSTPEDWMATARKIYSEQSRGHTFKDELAWKKLRHAPKWKQDNKKLNYHQVLMPDLSVDVHQQHQHQRLQAIGQQEQTLNQHHQQLHQLLQQPQDQLLPPPQQQQQQQQQQHQQAECSSTSTQHHSPRSDSPLTQTIEGSRSKRLRLDQDLERLIAQTTGHDSSHSIPVIPTDPLEKRQPDPSNENNEDQDQLDLNTFDSLDDQGHQSQRLVAQNLQLERELKRSQIEMNNLKLLMQSEADCPDQESVILLRLMKSRLKKRLLLDQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKW0</accession>
    <name>A0A5B0MKW0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016655</name>
        <name evidence="3" type="ORF">PGTUg99_029650</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKW0"/>
    <dbReference type="OrthoDB" id="2498269at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055334">
        <property type="entry name" value="PEX8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214:SF1">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="666"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="673"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="777" mass="88213" checksum="23F7F86F3E860CE2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLM5</accession>
    <name>A0A5B0MLM5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016996</name>
        <name evidence="3" type="ORF">PGTUg99_030571</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLM5"/>
    <dbReference type="OrthoDB" id="2510980at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="75" mass="8406" checksum="73CE17938BAB60E8" modified="2019-11-13" version="1">MPKRAAPECRPPPCQSDVLRSVFEEDNDAEDDAERELSLHSRVNRVFQMISGVRNKLPKTPSSSSKFLSHASPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLN6</accession>
    <name>A0A5B0MLN6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLN6"/>
    <dbReference type="OrthoDB" id="3364670at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="825"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="969"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1101"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="991"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1068"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1108"/>
            <end position="1120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1140" mass="129449" checksum="B6CF59180A5C3299" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMH9</accession>
    <name>A0A5B0MMH9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMH9"/>
    <dbReference type="OrthoDB" id="2518442at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="209" mass="25104" checksum="BFA97B1C05A8772A" modified="2019-11-13" version="1">MLKDIKHPTKYPEYMCKEKRRYMFDTLAAWKHAFKTLDLMFEYQFISPVEIKNLFQDEKMVKQVVHYIVGYHMNQFGLCYHYIMDNLTESWDWKDSLKFLSVLGCKEECIMNFVFLIGKVKAPVPNCSRRYQDRELENFDRSFSCEKYFSKFLDKSNDSKLSRSSLFSGTQYMIHPNYNDKEDIQEIIDKLVTQNPHLDGLIHPSRHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MND8</accession>
    <name>A0A5B0MND8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MND8"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="70" mass="7857" checksum="23C8AD47181FDEE0" modified="2019-11-13" version="1">MPSRPATIKEYDLLGPGSKKESDLDGQEIQENDLNRLGIQVTSFGTITKKMTLAIFKFINLLLENCTNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNN5</accession>
    <name>A0A5B0MNN5_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNN5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022781937" evidence="1">
        <location>
            <begin position="22"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="51" mass="5743" checksum="BC85DB871ED110C2" modified="2019-11-13" version="1">MMCFIGLIVLAALQLLFDVSATWHDTNCNSEAILWHINSITQISKSKLTLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPP2</accession>
    <name>A0A5B0MPP2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_029277</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2017782at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000486">
        <property type="entry name" value="Pumilio domain-containing protein c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000842">
        <property type="entry name" value="Pumilio domain-containing protein c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000475">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.2, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052645">
        <property type="entry name" value="Pumilio_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47093">
        <property type="entry name" value="PROTEIN JSN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47093:SF1">
        <property type="entry name" value="PROTEIN JSN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="501"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="664"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="PUM-HD" evidence="9">
        <location>
            <begin position="888"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="6">
        <location>
            <begin position="952"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="58"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="305"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1243"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1243"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1290"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1319"/>
            <end position="1329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1078138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1356" mass="144379" checksum="B2EBBEDA915D2BCE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MS19</accession>
    <name>A0A5B0MS19_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024404</name>
        <name evidence="2" type="ORF">PGTUg99_019297</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000446">
        <property type="protein sequence ID" value="KAA1078904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MS19"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="95" mass="10875" checksum="CFF9433DA1279F11" modified="2019-11-13" version="1">MMCSVRKLSREPFKIHENSLSSKSGLVKIIVRKAQLSVSIDLWMVSIVLTGHPERLTNSIVSRDSHFENSRLTTPSARHSNLLDLKGGYWKRQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSM4</accession>
    <name>A0A5B0MSM4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023303</name>
        <name evidence="2" type="ORF">PGTUg99_012714</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000446">
        <property type="protein sequence ID" value="KAA1078869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSM4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="67" mass="7222" checksum="5E5C83A2354E7036" modified="2019-11-13" version="1">MTEPTVNTGLFKHGLFGGRIEPDGDRHSLEAQGLSKGRLKEPVMIEWPHELGGPATLIPTTACKKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVW2</accession>
    <name>A0A5B0MVW2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ERF2_4</name>
        <name evidence="14" type="synonym">ERF2_1</name>
        <name evidence="13" type="ORF">PGT21_026298</name>
        <name evidence="14" type="ORF">PGTUg99_009907</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVW2"/>
    <dbReference type="OrthoDB" id="2507193at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF488">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="511"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="410"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="156"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="206"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="608"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="340"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="622"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1080957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1131115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="667" mass="75697" checksum="5993636A07F5F1C5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXK5</accession>
    <name>A0A5B0MXK5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000129">
        <property type="protein sequence ID" value="KAA1081721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXK5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="91" mass="9939" checksum="F05D5AB41FE60601" modified="2019-11-13" version="1">MSPRVEGPPVASARARHRAERCGAARRAPSTLSLWRARPDPAAWSPPIGVLTTRTAVIPLRALSAAFCQSQARRKVVGGRNLRTLEDLLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MY18</accession>
    <name>A0A5B0MY18_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1080769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="214" mass="24438" checksum="1DF810E89E37CAA6" modified="2019-11-13" version="1">MDPISLILDEQPPVLLHNVTYDMQTRMNAFAHRVPSKDGATIPNISVKQMVVVEQCYSTVRSLGEGSWKDNNYAPGLSHAGFDPDTGLKRPDFHKSASYQSSFNKEKQHQPNHQERRREKRWFNENGQLNMSNRPFGFDNKNHYQNFNNNNHNQFDYANYNQQNYNQHPYNSPYQNNQGNSNPNRSFVGGSKRFKGANSSFDSGGAKGGGECNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0N3I3_PUCGR</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="328" mass="35138" checksum="F9911D2310EB9DCD" modified="2019-11-13" version="1">MPTLTLSWTDLALFGLTAGLIGGIVLIGRSNSSPNVTGPHNLKKNAAPKKKKKAKKGGEPISSPPLSSPSAKRRESSAQSPPPTQPITTPESHPEPPSAVQSQAVEAKPATGPSPAQPQKAQKKKAAKKAADTINDQDFPPLASAKDESHNKTNQNKPKRPFAERHQQPVRKTIVDDMIDEDVQKPLKMARVMNIVNPESEAPLSPASDPEDGWEKVPDSKRSRTTGISISIGTGSSGQPAAKPKQAAEQATSKRQRQNAKKKEAAKALKAAEEAERLSHLSAHKREQERIRMNAQTRSPQPSATSSKPLGKKVASASVAEDGRLIWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0N406_PUCGR</name>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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            <end position="273"/>
        </location>
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        <source>
            <dbReference type="EMBL" id="KAA1082870.1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5P3</accession>
    <name>A0A5B0N5P3_PUCGR</name>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1084541.1"/>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="KAA1084541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="74" mass="8642" checksum="47686FAD8F5FC4F1" modified="2019-11-13" version="1">MKMIYWSHENLQQGKHWFQHPIDVFFSHGLQAFHLMTWSLTIVAHVMVIRPSLRASDTGEINSPDNGKIKMLHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6C0</accession>
    <name>A0A5B0N6C0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
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    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
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                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="2508431at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137415" evidence="2">
        <location>
            <begin position="24"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="236"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1084805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="749" mass="84284" checksum="AA26E365A95A90C3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8C0</accession>
    <name>A0A5B0N8C0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000767</name>
        <name evidence="1" type="ORF">PGT21_000781</name>
        <name evidence="3" type="ORF">PGTUg99_002719</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000198">
        <property type="protein sequence ID" value="KAA1064096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000111">
        <property type="protein sequence ID" value="KAA1084976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000256">
        <property type="protein sequence ID" value="KAA1118315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8C0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1064096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="120" mass="13083" checksum="434B912750554422" modified="2019-11-13" version="1">MTTSYSSASFEAASKFQITPKALGGKAALTVCSSSNDSDGGMLLGTKHGGEETEGQFQGFSQYSNAQSFIRPIEQTIPVRISSPIDSMNVLKTQFLIQEQSIQAYWKEFRSTCSHWSLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NB49</accession>
    <name>A0A5B0NB49_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025254</name>
        <name evidence="3" type="ORF">PGTUg99_021090</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NB49"/>
    <dbReference type="OrthoDB" id="2500224at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="322" mass="37461" checksum="281807272C401970" modified="2019-11-13" version="1">MQTTIVEEQSANKKFFLTEGDLVYAIGSVTNHNSVGFTPFLDENIRKLRSPLPLTIFNRKWQQRAMAHHLDRLQRSNELSNDKDKSGGYKGFAFVQEWTQTYAQWTQNHRSFRKTLHDVYKIIEFSKLLGTHKNNCDDITEEFGFMVAFRYDMEVRNNTFSHRITDEDVKNAIPDISQRKESFVQHCYNQCRNFGELEWEENLYAPGHSHANHDPLTGCLKQQRSNQFHNNQMIHNPYLQQQQQYGGNNFNSHNQNNYNNNQNRNYNSGGGGSSWNQNYNHNNFGNSDRKKRPRGGYRGSNYSDNPQGRADNGQGKKGGSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NC63</accession>
    <name>A0A5B0NC63_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010711</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NC63"/>
    <dbReference type="OrthoDB" id="2497757at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="16940" checksum="9A669D63D0AB63F9" modified="2019-11-13" version="1">MEEDLSKVTISSAQTTEETNRHVVDNLPSLGPIGVKVNGYDCEGSLCPGLLFNFISEEEARKVDFDVHPQDIWLPGVCEEDIKITGIALATVAMEGISETTSFLVTQKNGPLIFGKPFMNDFLAEISYNNQLAPTLACFGSGGTTHLRIPFNLTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND74</accession>
    <name>A0A5B0ND74_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009956</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND74"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="352" mass="38077" checksum="72B46F0A027FF193" modified="2019-11-13" version="1">MTPLFNTTPRLFKVIACWFDSIGFLRLEVFINPNRLVIHLKLFDSTSAFSLPSSLFNSSSSILSCCLVDSLATTTIRSLGIFRSPIRRGLMPPIELYIATPLACYPHQRLFDGASDQFEHLVRTLQPWQLLLPPLSIPNDSCQLQLPPSACNIPTPSSATTLSLTICCCCPSSSHSNCGSIDLFAARSTCFTAPKAGQLTSTTNNLKKHWRSQSIIISKNDRTTTASQADPPSCPIPSKTSSAHRLCNLFTEIAPPIDLITGLATTLPPQTPGLKATVRSNVILILPSTAPHIDLNRSTSTSLHLHLQRLHSISTTSSPGSIPISTASSFYGILCSINPDLNSIITLIDPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDL5</accession>
    <name>A0A5B0NDL5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDL5"/>
    <dbReference type="OrthoDB" id="2507862at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052833">
        <property type="entry name" value="Telomeric_DNA-bd_trans-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807:SF1">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="976"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="642"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="679"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="692"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="710"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="720"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="736"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="770"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="810"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="822"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="846"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="993"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1083"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1101" mass="120292" checksum="88B5602BE22C5604" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFE3</accession>
    <name>A0A5B0NFE3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylethanolamine-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFE3"/>
    <dbReference type="OMA" id="FFKSTHQ"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF82">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022725139" description="Phosphatidylethanolamine-binding protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="24442" checksum="2CB8C0A4E18446F4" modified="2019-11-13" version="1">MNTLWTAFLLVACVNFLATAKSSHRKRDVCEVQGNDAAYDACGMKKAFGGSTVYPELFTTFSPKGALYVRYDNVVVGGAQQLAPHRVSAKPSLSMHFFQGPQTAAGAQLLSKKFAVLGLDFQPEGKLTKVIWLQSAMDIDRNTGSLTSTVPPIIPYKSPNPPQGTGTHEYVILVFEEIGAGLSGFLKKNPQVRAILSGSFNLEDLLVQSKLRNSLVAASFFKSTHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFG7</accession>
    <name>A0A5B0NFG7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFG7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022658263" evidence="1">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="50" mass="5210" checksum="BB82E098FE9927E1" modified="2019-11-13" version="1">MLLVLTSTVALSVAISTAFPVLIPVPVPITCYRPGNADTPRFVEKPAAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFU7</accession>
    <name>A0A5B0NFU7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFU7"/>
    <dbReference type="OrthoDB" id="2496728at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="1">
        <location>
            <begin position="188"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="446" mass="51905" checksum="BD2E50058BFA5B8F" modified="2019-11-13" version="1">MRITLQNFGHEFQSIVSELINAGHNDNEIRQFLQENHSIIVSQRTLTRRKEDWGLILHASQQMADTEEHIKKYFDQGLTYSQIHHALTTSHNYTHSKHTLQRKITAMQLSRRLDDLDTARVTIEAVVSCVMHLHLTPEGRNVGYRRMRQLLQTKFGITLHYITVALINRTLDPDGVENQAKRVLKRRVFKTPGPNYIRSADGHDKLKKFGITLYGFIDAWSRKILSIYVHITNNDPRHIGYYYLQLVKETGGIPRRTSTDKGTETIHLAGHQINLTQQYNEESIDPTQSHLFTKSTHNQKIECLWSQLMKQYNSELINKLFTAIEESFYDPQDPLKQLLFIYLWVPLVQRSLDDWTNNYNTYKRRLDKKSSLPTRCSADWCFNYPEEQGGEQGLIKVPSEAADALEKDFYPEGDELLRTTPKWFSDIIAELQAAFDLAIPIFLDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NL81</accession>
    <name>A0A5B0NL81_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NL81"/>
    <dbReference type="OrthoDB" id="2499206at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="159" mass="18068" checksum="16E6AA303DEA2E5C" modified="2019-11-13" version="1">MFLRGAGFVILLRIMLILLMIHLIIPSARPANVVGPQQCTNGFALASYHSDAVSCKTSQNVIYDCKVSQCFATSDTSQHAKPYSEFVFEKCHRIDASDHPTKSTSTIHPAEFYPTYNEKNWLEIHGSPPLQPGRRRYQCYTSEAQKLNTNRPWDTIIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNB8</accession>
    <name>A0A5B0NNB8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_014368</name>
        <name evidence="3" type="ORF">PGTUg99_014624</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNB8"/>
    <dbReference type="OrthoDB" id="2512234at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="667"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="696"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="727" mass="75959" checksum="81BD2AE95F02C4E7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQ61</accession>
    <name>A0A5B0NQ61_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011782</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQ61"/>
    <dbReference type="OrthoDB" id="2333993at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="364"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="845" mass="92508" checksum="7F075A22FBE4535E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQB3</accession>
    <name>A0A5B0NQB3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YDG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_034809</name>
        <name evidence="5" type="ORF">PGTUg99_036105</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQB3"/>
    <dbReference type="OrthoDB" id="2270193at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.280.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase UHRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.280.10">
        <property type="entry name" value="SRA-YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036987">
        <property type="entry name" value="SRA-YDG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003105">
        <property type="entry name" value="SRA_YDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045134">
        <property type="entry name" value="UHRF1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14140">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UHRF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14140:SF27">
        <property type="entry name" value="OS04G0289800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02182">
        <property type="entry name" value="SAD_SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00466">
        <property type="entry name" value="SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51015">
        <property type="entry name" value="YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YDG" evidence="4">
        <location>
            <begin position="193"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="34"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="400"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="444"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="518"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1076126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1091497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="586" mass="63340" checksum="769255A9E06364DC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQM4</accession>
    <name>A0A5B0NQM4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_019550</name>
        <name evidence="8" type="ORF">PGTUg99_018057</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PheA/TfdB FAD monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000177">
        <property type="protein sequence ID" value="KAA1125548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQM4"/>
    <dbReference type="OrthoDB" id="1716816at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02979">
        <property type="entry name" value="PHOX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012941">
        <property type="entry name" value="Phe_hydrox_C_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038220">
        <property type="entry name" value="PHOX_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050641">
        <property type="entry name" value="RIFMO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004:SF4">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07976">
        <property type="entry name" value="Phe_hydrox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="12"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Phenol hydroxylase-like C-terminal dimerisation" evidence="6">
        <location>
            <begin position="451"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1090972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1125548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="661" mass="72994" checksum="C935CBA7B446E130" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NRP0</accession>
    <name>A0A5B0NRP0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003359</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NRP0"/>
    <dbReference type="OrthoDB" id="3019291at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="391"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="877"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="898" mass="101655" checksum="7B21C15399314483" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTL1</accession>
    <name>A0A5B0NTL1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome activator PA28 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_033953</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTL1"/>
    <dbReference type="OrthoDB" id="6591885at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008537">
        <property type="term" value="C:proteasome activator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.180:FF:000002">
        <property type="entry name" value="Proteasome activator complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.180">
        <property type="entry name" value="Proteasome activator pa28, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003186">
        <property type="entry name" value="PA28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036997">
        <property type="entry name" value="PA28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009077">
        <property type="entry name" value="Proteasome_activ_PA28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036252">
        <property type="entry name" value="Proteasome_activ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660:SF2">
        <property type="entry name" value="LD45860P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660">
        <property type="entry name" value="PROTEASOME REGULATOR PA28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02252">
        <property type="entry name" value="PA28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47216">
        <property type="entry name" value="Proteasome activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome activator PA28 C-terminal" evidence="4">
        <location>
            <begin position="582"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="534"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="564"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1091450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="727" mass="81485" checksum="6C0D4463335F3720" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUC1</accession>
    <name>A0A5B0NUC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007161</name>
        <name evidence="3" type="ORF">PGTUg99_017124</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUC1"/>
    <dbReference type="OrthoDB" id="2336871at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053216">
        <property type="entry name" value="Appressorial_penetr-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34587:SF2">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34587">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137562" evidence="1">
        <location>
            <begin position="20"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="244" mass="26781" checksum="4DE24F5405A9B314" modified="2019-11-13" version="1">MRLYLHLFLAAILAQSCLGETVTRNQTHRLLHLDSRAIQNASFHTGNPTGNQSPSLTSHNNFINFCISKHALGARLMNGTQSRNNYSCNSIPMGMIVAPEQMPSCKFKHPHNLSKLKANKQFNIILEIKKMVTGNFVNPNASYFAAPQRLSSETKEIIGHAHVVIQSIPSLKSTAVLDPRKFVFFKGIDNPVSKDGTSTVVVTRGLPAGTYRLGSILTAANHQPVLAGVAQRGLFDDVVYFVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZN1</accession>
    <name>A0A5B0NZN1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028625</name>
        <name evidence="4" type="ORF">PGTUg99_033048</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZN1"/>
    <dbReference type="OrthoDB" id="2505239at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="461"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1121596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="606" mass="66515" checksum="1DB6BAACD0C2C9DC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P042</accession>
    <name>A0A5B0P042_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018336</name>
        <name evidence="2" type="ORF">PGTUg99_019290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000479">
        <property type="protein sequence ID" value="KAA1071211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P042"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="164" mass="15191" checksum="7A8DF9C04C529797" modified="2019-11-13" version="1">MNFSMTYMALLGGGGGLLGKLGAAGLPAPAKGGAPAKPAPSADAAGAVAGGDDSKPAADPPAKTGGDSTPAADPPAKVDSPAPSATPPADSPAPAKAPDTAAPAKAPDTAAPAKAPDTAAPTKAEKLKKASMIMDTISKALSAGGLSLSSMITGAVGTGLKGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1H3</accession>
    <name>A0A5B0P1H3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020124</name>
        <name evidence="4" type="ORF">PGTUg99_017092</name>
        <name evidence="2" type="ORF">PGTUg99_031296</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000004">
        <property type="protein sequence ID" value="KAA1137977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1H3"/>
    <dbReference type="OrthoDB" id="2495475at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137619" description="ZP domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27795" checksum="3A788097B82D4200" modified="2019-11-13" version="1">MVLHRVIFIAVICLLNAPSSLQLTKPVPLTDIKCSKKRQLRPGDCARAYQKIIYDTDLTLDQSEITLEKVSGSCVTRIDNPKFLNVPKEIIEDAFNQVVTKCDGVPGHANLTSFDGVRVLVRHHTRPSVYGYEDDTELNQSICFRDSDTQDIVEEDCMEAYRLLPTDTAGHFLSVEHHVQGNSIGLTFKTCLVSVWTSDGSKIIVLKGDMERLFGKLMQQCKVNGKGGTLITEGAQGKNGKVNLQVSTPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2V9</accession>
    <name>A0A5B0P2V9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2V9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="98" mass="10600" checksum="1C2FDD2E90236209" modified="2019-11-13" version="1">MQVNLFPSPIQQRSTFKSSQYSEEHRYRPAQQSDLKVAGSRVSSDIHSARRPIHTHSAKPPIRSSQNTIRVPAINGTPSSGQPAATTPPDAPGSTLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4E1</accession>
    <name>A0A5B0P4E1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1095983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4E1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1095983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="175" mass="19595" checksum="C0AF8238BA8EBDFF" modified="2019-11-13" version="1">MSSVFRLPKTIQLPASQSTITFRPKIPNRLPTNNTNGLPIRKTCCEGCSIPRFFMIRRSLTEPKSNGFSCSSFHPPDLRLFTHHRNLHNVQSFHLETDLQREISVIRFIVRTRTVAACEDDTDLAISLTFACGLIVRRILIIVSYPTLAACKGSFSLVSTALTGSKPLRVDFLAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PC28</accession>
    <name>A0A5B0PC28_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_029606</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MNN1/MNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PC28"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="78"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1098164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="601" mass="68613" checksum="BE99C040F909ED34" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCL0</accession>
    <name>A0A5B0PCL0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034456</name>
        <name evidence="3" type="ORF">PGTUg99_017802</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000176">
        <property type="protein sequence ID" value="KAA1125653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCL0"/>
    <dbReference type="OrthoDB" id="10550145at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="378" mass="41694" checksum="41E4B5D5E532066F" modified="2019-11-13" version="1">MPTPSRILCAETQLEEESILDRPPRKAPIHLDYSLYVKHLNNADFPHAKIVAAPNSWSVISPLRELGAMSIDMQSMTWKHFQALAIDHLGQHQPALAGVLSAAKDVGALHWLPSISGHDQFGESNMKKKNKICGHLDFLAFATQAYDAFPNKVVFKVVMLGPMPVNQAHPQPKKTSRLHAPADGVDMNEANKTGAQGKVILDTNQPKLRPWGEPEEPARTSLLRAPKRTISSTGDRSGKHAKMGPNTPEEAPTARAADDPFEIEVIQRPFPSCPVRNAPTARAHQNANSVAFRSPLGPAGPKLEAVDMETYLTVAHIDASDHATRRRLNDHGIVHWSFFRRATEEELLTLGFPIGTARLLCEGVPRLEYYVEMQSVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PE86</accession>
    <name>A0A5B0PE86_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028786</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PE86"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="52" mass="6210" checksum="8D210F0798E1FB4E" modified="2019-11-13" version="1">MFNGSRRRRSTLLELRAQVIVLAPRSGCKRRFLSLLESEVNYRPLDPRLQYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEM9</accession>
    <name>A0A5B0PEM9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEM9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="130" mass="15082" checksum="DB95C665B2C49386" modified="2019-11-13" version="1">MSRWCVRARYHTTHVFQLRPVILRLDEVSFIHIGKSQSAKSPDRVRSPRRVPHRRRRSRQKDTVRLANMKISYCNSIHSVVSRCLRLTVADYAGSWDHVESVWTSSYFKASILFLSEILPAQALTPVFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFB1</accession>
    <name>A0A5B0PFB1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Importin-alpha export receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CSE1_5</name>
        <name evidence="2" type="ORF">PGT21_003727</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFB1"/>
    <dbReference type="OrthoDB" id="2501327at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005043">
        <property type="entry name" value="XPO2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03378">
        <property type="entry name" value="CAS_CSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-2 C-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="153" mass="16524" checksum="02297C110F9A4D55" modified="2019-11-13" version="1">MEGVLLPAVASTPPKQRRVVALGHISLLTRARTLQCEAESRLYLPVLISVLRLFTLPQLKPTEDTAGENSELNVTDLEEHGYQVGFSRLGASETIGKRDPFSAMVDPRDALASGLVKAGEAQPGKIPALISQVPSEFATPYLQWLAAAGYQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFB5</accession>
    <name>A0A5B0PFB5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFB5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="62" mass="6891" checksum="49B26C0BD36969ED" modified="2019-11-13" version="1" fragment="single">SHAHTRYLTPSNDSHQRSPHTVATNECTGERQPPTRPSVNGNLHREHPFSIEAVTHVAIRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PG23</accession>
    <name>A0A5B0PG23_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_014683</name>
        <name evidence="5" type="ORF">PGTUg99_011922</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PG23"/>
    <dbReference type="OrthoDB" id="2505789at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137759" description="DUF7872 domain-containing protein" evidence="3">
        <location>
            <begin position="32"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="4">
        <location>
            <begin position="254"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1095274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1099610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="480" mass="51773" checksum="90532A685E4E41BD" modified="2019-11-13" version="1">MRRTQSKGLQFVKLTIVCCASLSLLSVPSEAKVPDHEPVSHHERRSLAPAGVPPSPAPAPVGATPASVSAPTPPSTPSDPHACQKLPITPDTWKLLDIDNTLQRLPGGQNMTIAQYAAANGAGNFICGIGENCNAGQLCHPVKAPAWQVLYATQEYTAFTNALTDSINYALSQLQAIAASMSQDLVENIADSRLEVDLKWLFTDMYRGIAYGATALFIISFTAKSFGGIFEVAAMILFLTGVGAGIAAWFQGAPKTASFVSWSKLSFYITQAQGELATRITEATEKTFKAGITSENGLAKILAGGHFFTPASPVGIMETTEKSIMQIVQGRLLARLLRAQNAYITISPDDCHGKGPGGSREGNDVLSYCDPGQKLLFNVVRAHKKKTINTIYGASAVTNKYGFTVEFIVKNSWECQKKYGNFEHDVFADASKVSPNDMMSTDCIINLPVCDMRDDYVRNLKFSRHKTTTYACRKGGGLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIL8</accession>
    <name>A0A5B0PIL8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIL8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="58" mass="6158" checksum="5090843AC22437D1" modified="2019-11-13" version="1">MAEAPFFVVAGQKSAKKQPCPKPLSDLSPSTGQPIGTYQLEVNPQGQAYRAKVESPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PND8</accession>
    <name>A0A5B0PND8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_022390</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PND8"/>
    <dbReference type="OrthoDB" id="2160599at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013942">
        <property type="entry name" value="Mediator_Med19_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="221"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1101489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="374" mass="41160" checksum="BE7A18D36F0948D1" modified="2019-11-13" version="1">MQNLQPNSSALHTQDHYHHHQKQQQQHPSQETTSTPQAGTSSTSNRQLHPNPKTLYFPVIDQGHLPAAPISGSHDLLSLFNVSSMYDWFVKPYLKPLDQTNKLGPTNNHHHHHFPNTNGDLKGKGKADIDLTGLSPAQLENLGQKRIKMEKSYGHFVADVGGRNSIKKDHQLETWISDPNFQEASVPNFLKPFSNEALSQAFTLQPGILPGFDSSVWVAEEGGPKKKRKKRKQGEMLAGSNPTPAAAHLQQPHQSPNQQTTMTTTTTTTTTSGTPTQYAVSSTMQAQPTRPLRASMVDSSSHLNSHPHSSHHPAAQASHLHLHQNQQQQHNSPGMFCREDDYRKKKRVKIGNPAAGKTSGSGSSGPNHQVALQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNN5</accession>
    <name>A0A5B0PNN5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin carboxyl-terminal hydrolase 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">USP7_37</name>
        <name evidence="3" type="ORF">PGT21_036732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNN5"/>
    <dbReference type="OrthoDB" id="2505769at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="347"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="600" mass="67738" checksum="B4DE047240F63387" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXL9</accession>
    <name>A0A5B0PXL9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015261</name>
        <name evidence="2" type="ORF">PGTUg99_015540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000345">
        <property type="protein sequence ID" value="KAA1098856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXL9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366459" evidence="1">
        <location>
            <begin position="22"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="151" mass="16531" checksum="24C739E3DB1D4CA5" modified="2019-11-13" version="1">MLLAGTILMMFSIISLPGAFAAATLPPVSSSMKISRNVGELVDKRIESNIQEKWKAIRAGVITKDPGSPRATKVKVFKDLPQEGNKISRVAQVEALASFKLARGLSLTRDVKSSELAISRAEEEAFKAIEEVAKERLKARPGLKLSWKSWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYN3</accession>
    <name>A0A5B0PYN3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYN3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="7876" checksum="FB82D3C6EF6B1D2D" modified="2019-11-13" version="1">MRVVYDTSPTCPVGAPSRDGASNRNLKPAPKVAPPPPTSSRANRRQGIIFNVQALPQELKALQGQSDSKNPAGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2W0</accession>
    <name>A0A5B0Q2W0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2W0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7617" checksum="4B2AB47667C90FF5" modified="2019-11-13" version="1">MADWLAKDALESPSTQHLEVGSNCKKVHRQALSSSSPKLPPPSNLPIVSSSLINQLASGHYQRPFFLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4R2</accession>
    <name>A0A5B0Q4R2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4R2"/>
    <dbReference type="OrthoDB" id="10479387at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="738" mass="85105" checksum="5571E7EE9F7DC1CB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q504</accession>
    <name>A0A5B0Q504_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">26S proteasome regulatory subunit RPN3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPN3_2</name>
        <name evidence="6" type="ORF">PGT21_006902</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q504"/>
    <dbReference type="OrthoDB" id="1713558at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000009">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013586">
        <property type="entry name" value="PSMD3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057985">
        <property type="entry name" value="TPR_PSMD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08375">
        <property type="entry name" value="Rpn3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25573">
        <property type="entry name" value="TPR_PSMD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="5">
        <location>
            <begin position="288"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="100"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="507"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="527"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00075103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1108291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="542" mass="60839" checksum="1288A1C2445C8D78" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q582</accession>
    <name>A0A5B0Q582_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q582"/>
    <dbReference type="OrthoDB" id="2506242at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="318" mass="34831" checksum="F7CA2074A7B436DA" modified="2019-11-13" version="1">MNRFRPSPPSTDTSPGTDRSFPDLDRHFRPPPYNHNPPHFPHSVFHPHPPMFHPPGPVFPPFGQGGGFRGRGFGGARGFGGGRGGGFAGGRGGGFAGGRGGGFAGGRGGGRGRGWVPSAEFRVQGQANRFYHPNGPAAGVPFGHGHPPVGVILKFLNSNRAQVDINNFLNPPPNPSGTRRQSPIAVPSTPSIPANADQEPSHISSVLSVDSFHTAEDFLLPQIRVPSPSPAGMYDPDDYHHLARHQVEALLDRRAAPERFDRYEFTPQHFTDFYQSATTALVHHFTRHPAANANERQDRVRLFRFLTRRYRSMTRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q682</accession>
    <name>A0A5B0Q682_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q682"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="115" mass="12924" checksum="6779FE39B5D09745" modified="2019-11-13" version="1">MSTAEFPALLSSHTMEAKAITVCELLQSLPCKMTPKSFLLHFLQSDNLDLAYRPRYWAESAVDSTLVLVKATTEAEIIWSPPPQHLQIIKMGPNVAAKAFDTADVYQDQEHIPYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6E8</accession>
    <name>A0A5B0Q6E8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPL43_2</name>
        <name evidence="5" type="ORF">PGT21_025544</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6E8"/>
    <dbReference type="OrthoDB" id="10258345at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000002">
        <property type="entry name" value="60S ribosomal protein L37a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1108780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="92" mass="10187" checksum="B370A2956146A74D" modified="2019-11-13" version="1">MTKRTKKVGVTGKYGTRYGASLRKQIKKMEVTQHARYTCTFCGKDTVKRSAVGIWDCRSCRKVIAGGAWTVSTTAAATVRSTVRRLAELRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7E4</accession>
    <name>A0A5B0Q7E4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027559</name>
        <name evidence="3" type="ORF">PGT21_028322</name>
        <name evidence="4" type="ORF">PGTUg99_008054</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1128941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q7E4"/>
    <dbReference type="OrthoDB" id="2514094at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137937" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1128941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12447" checksum="50EA0F016D4DB52F" modified="2019-11-13" version="1">MQIIEFFPVFISVILLGTALEVKTKKPDAFKCDKNQKGPKAICILTPGGEQPSNYWVQLAESSPPTYNCNTLGASYQNVTGGCCLANFAVNSSPIKQIDVPTFNANCNFASKPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9F8</accession>
    <name>A0A5B0Q9F8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9F8"/>
    <dbReference type="OrthoDB" id="2502203at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="525" mass="57267" checksum="F4623E8A4D121AD5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9K6</accession>
    <name>A0A5B0Q9K6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004916</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9K6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1109966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11659" checksum="9F57750BA3C4B87A" modified="2019-11-13" version="1">MVLGSFCFMIEDMGKYAAQSHHLYSVDKPSGKRVLSTVLFNSCFGTISTKRAFRRLHCSRWNTPAPGLVKVAGSYEPMQPEIRQQCTMDKESTKWARCLPPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB73</accession>
    <name>A0A5B0QB73_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_018632</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QB73"/>
    <dbReference type="OrthoDB" id="289162at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19494">
        <property type="entry name" value="Elp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008728">
        <property type="entry name" value="Elongator_complex_protein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896">
        <property type="entry name" value="PAX6 NEIGHBOR PROTEIN PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05625">
        <property type="entry name" value="PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="110"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="395"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="450"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1110345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="461" mass="50333" checksum="345D65BA323B5D5B" modified="2019-11-13" version="1">MSSFKRNLKPNNPNQAPSSYPTGFHPSPYNGVPTASTGIASLDDLLGGGLPISSSFLIDEDEDGGYGKLILRYSIAQGIVSKQHLIVVGSSLDEGGDPEKIIDRLMSLDESDEPLEPPSSNQSQKSTARSSGLSAEKEKSTAPSDAADDDDEEEEDDDDEVNADKVKSQRMKIAWRYENLGHHHSEAESIYQQSRCHVFNLSKTMKLSADQRSRTDCIDVKNLEGLPTSLLAQIKQIIEERRKASSSLPSFRIVIQSIGSIGWPPMEDFMIFRFLKELEMLLRSTDCPKIAMISFPSAYRSPDLAKMLPWATSGSLSLQSFAGDEKSQAAFPNHQGAVHFIRLPSPSSLSPPATKLSVIRALGGGSEAGIGSNLGFKLGRRKGFRIEMMGLGLDIENENPPEEASTTHQPDKPSEVPVDGLDGLAIVNPSQVKKKSAKPRSRVRFGDPSQDSHDLLKNTDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBM2</accession>
    <name>A0A5B0QBM2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin carboxyl-terminal hydrolase 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">USP7_12</name>
        <name evidence="3" type="ORF">PGT21_019243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBM2"/>
    <dbReference type="OrthoDB" id="2495097at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1110364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="198" mass="22039" checksum="B737D08B26E95148" modified="2019-11-13" version="1">MESGIMKGVGFQPGSDQSSTAGTYARRANLSQEILDQDNYCWDQLGDHNQFLCNRVRHFSKQSFKDNAKIIESFGIPSWSNSEWNDFEQETNGIFSSAITTHSDFSNEPHMDEDSNPWTYGLFSYINQSTGKPVLPSSSVPGHAFRFPDFNCQIDFGTSPGIIELLWASNSVKHHTLHPPPSLKSTAGITHSGSSFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCU0</accession>
    <name>A0A5B0QCU0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dyp-type peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_035917</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DyP-type peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCU0"/>
    <dbReference type="OMA" id="WINNANF"/>
    <dbReference type="OrthoDB" id="3207336at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049509">
        <property type="entry name" value="DyP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048328">
        <property type="entry name" value="Dyp_perox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006314">
        <property type="entry name" value="Dyp_peroxidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01413">
        <property type="entry name" value="Dyp_perox_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521:SF4">
        <property type="entry name" value="DEFERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521">
        <property type="entry name" value="DEFERROCHELATASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21105">
        <property type="entry name" value="DyP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20628">
        <property type="entry name" value="Dyp_perox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51404">
        <property type="entry name" value="DYP_PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="DyP dimeric alpha+beta barrel" evidence="10">
        <location>
            <begin position="10"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Dyp-type peroxidase C-terminal" evidence="9">
        <location>
            <begin position="296"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1111036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="54568" checksum="731CECF374413D65" modified="2019-11-13" version="1">MSSNKVDLKNIQGDIIIGLQKRVEAFWFCTLKSESNSVEGFRRNLKDSLLPLITTTQDVLDTQKKINDHKRNKRSEGGNDLLPITQVNLGFSFAGLKKLGLKLEEIPTGGDGVFSKGQKVDAVDNLGDPVDPKTKKLKTWSKDFLHEANSIDFVILITAPDCKLLDQKLDQLRKNLSGFLASSFVRKGNVRPGNEVGHEHFGFLDGISTPTIENINAQPGDKKSGIVKPDVVLLGQPASGGNTNNLDPKQAWIKDGSFMAFRELQQLVPEFQSFCDESAKKLQNPNVSGDFIGARIVGRWKSGAPLTLTPVQDKPELNRAQDFDYSDELKQERCPYAAHIRKTNPRNGIAGADPNTAVLPHLMIRNGIPYGPELTAEEKKAKKTKENRGLLFVSYQSQIESGFQFVQKLWCNNTDFPAKTPAKVTPGFDLLIGQTADEKPRVAQNINPLGIAGTTDPNNIVTAPAHFIVPLGGEYFLMPSIKAINEKLGLSSRSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QD34</accession>
    <name>A0A5B0QD34_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036891</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QD34"/>
    <dbReference type="OrthoDB" id="10492965at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="8345" checksum="09C33D47F83A92AF" modified="2019-11-13" version="1">MPYVYYPPNIKVMAIRMTMEGKSRYVIRQALGYSISNQSFNRWQELYEQTRRVIRNPEEYERPLAGQEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QE30</accession>
    <name>A0A5B0QE30_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001741</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QE30"/>
    <dbReference type="OrthoDB" id="10050400at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040201">
        <property type="entry name" value="Mrg3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142:SF1">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="392" mass="43659" checksum="653C6E38907C5206" modified="2019-11-13" version="1">MNHQLIRRTLVVGLSRRSTPRLNHSYSTTTSNSNFKRTTATVGLILGIPLTAYLIYRSQLPQVYPDSIQQLLRQALVAEHRLDHRKSEILFRQAYEQSKQLVRSGELDWLKSSAIAIRWAASLEGTARVDRAIEVYQLVFDDLRLALPGLSINERLRTIEIAQKLAQLFPSSAPPGKRPELVEKKIEECLTFSVEEILKIQNKDGATPPDHHQLPSIDQQLEALESLPSWVGRLQIASCFETLGKFYAQRGQIDFALPLYLQTLNLLLPPPSDKDTNRSRSSPTHEAQCQAATVMNNIGQLLFSRSETERAADGAGERLRLNAAQWLGQSSALAQRVLEEHHQVPLDVPGRTALADLPIGALAECRDAFIAANANLNSLNHPPTPKNSSPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFT3</accession>
    <name>A0A5B0QFT3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Proteasome regulatory particle subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPN8_1</name>
        <name evidence="6" type="ORF">PGT21_021826</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M67A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1112070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFT3"/>
    <dbReference type="OrthoDB" id="10256771at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005838">
        <property type="term" value="C:proteasome regulatory particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08062">
        <property type="entry name" value="MPN_RPN7_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000013">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033858">
        <property type="entry name" value="MPN_RPN7_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF7">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="5">
        <location>
            <begin position="97"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="397"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1112070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="440" mass="48191" checksum="BE8D3D401CA29A2C" modified="2019-11-13" version="1">MRTEFEVTKRDPPSRAYTGRAISARRRARAISTKAVSESVWAYVRALIDDRKSPTTHTHTHSNHLTKYTQKKKKKLRIDTMVAATSEEMVALSTTQVIIHPLVLLSVTDHAARVAKGGSKRVVGVLLGQDLGSTLNVANSFAVPFDEEEAGPLSSSSTASQPPSWFLDHNFVEAMGEMFKKVSAKEKLIGWYHTGPKLRATDLEINEVFKRYCPRPVMVIVDVRADRGVVKGSVTDAYFAIEEIKDDGTATQNTFMHVPSSIEAEESEEIGVEHLLRDIKDLSTGTLSTRVTGRLNSLRALQARLGEISEYLEGVLGGKLPLNHQIIYNLQDIFNLLPNVDLSPSASSAANDRAKPLTVVTNDQLLVVYLSSLIRAVIALHGLVNNKLENQNSANPIEQLDHSQPNPNSPTTNPTNGTAPTNNPDANLGPDKKHPSSGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QH87</accession>
    <name>A0A5B0QH87_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QH87"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="8014" checksum="3C1AA449D3823922" modified="2019-11-13" version="1" fragment="single">LTLNPNPYPNPNPNPNPKPNPNPNPNPNPNPNPGPSQDQAWTTWTTSGPSQDQGNPNPNPNPNPNPNPNPNPATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJR5</accession>
    <name>A0A5B0QJR5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GDP/GTP exchange factor for ARF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GEA2_4</name>
        <name evidence="3" type="ORF">PGT21_033143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10258608at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056604">
        <property type="entry name" value="GBF1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23325">
        <property type="entry name" value="TPR_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="2">
        <location>
            <begin position="659"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1569"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1576"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1589"/>
            <end position="1609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1639" mass="181284" checksum="91AC703D662B2B23" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QK03</accession>
    <name>A0A5B0QK03_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-actin-capping protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CAP1_6</name>
        <name evidence="5" type="ORF">PGT21_031245</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the F-actin-capping protein alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QK03"/>
    <dbReference type="OrthoDB" id="340550at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.60">
        <property type="entry name" value="F-actin capping protein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002189">
        <property type="entry name" value="CapZ_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042489">
        <property type="entry name" value="CapZ_alpha_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01267">
        <property type="entry name" value="F-actin_cap_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00191">
        <property type="entry name" value="FACTINCAPA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0117">Actin capping</keyword>
    <keyword evidence="2 3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="160"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1113423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="358" mass="38927" checksum="777A87052D9234AE" modified="2019-11-13" version="1">MEASLKDRLAVASALILQSPPGEVNDVFNDVRPIVGDDSELERGLLPALAQYNTEQLTLVELPNAKIPAMICSAAIVDQGSSSSSAAASLKEGEGSSRYLDPHSQQTFVFDHLRLSASELAPYVPPDAQMEDTRAELQALAEAYIKNHFQNAVSTVFSLPSSDAPDSSQLDQAGQDALVPPAAHPPLLTPSESVPQLDTAGQPASSSTDHPIDQSDPQISPASSSSSSHKFKLFIVGNKYNPTNYWTGRWRSIYEIDMDSGKIEGSISVNVHYYEQGNVQLSTKHLPKIEVPSPTNNKSPKEIIKAIQTAESQYQAGLNSAYSDLGEHTFKLLRRALPVTKQKINWNNIKSRIVLDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN31</accession>
    <name>A0A5B0QN31_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN31"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="11191" checksum="D13EB3D9BFA1D435" modified="2019-11-13" version="1">MDAYATTILDTQESVLQQNIHPQEDPFRQAGTSCVLAEVYPPPPSYEYGVQTCLARPGRHVLAEQAQAIAIPARSDSSKYQLDAIEWPPMPLPKLRRCPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQQ8</accession>
    <name>A0A5B0QQQ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_037111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQQ8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="92" mass="9561" checksum="2959EE1F287519AF" modified="2019-11-13" version="1">MKPGPKTVPRPDPANVAVWVGSGHKVNIAAQTRPTWGSRRVRVGSGRFAVTLASGGVADASETPGAEVATSESKVFSQESINVIHIGSRLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVA1</accession>
    <name>A0A5B0QVA1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021706</name>
        <name evidence="3" type="ORF">PGTUg99_036920</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVA1"/>
    <dbReference type="OrthoDB" id="2496480at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="738" mass="84188" checksum="60C53B56D6D787FC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXI1</accession>
    <name>A0A5B0QXI1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026042</name>
        <name evidence="3" type="ORF">PGTUg99_010771</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000048">
        <property type="protein sequence ID" value="KAA1134636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXI1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366550" evidence="1">
        <location>
            <begin position="33"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="123" mass="14009" checksum="25C27D84253FEF3D" modified="2019-11-13" version="1">MWSYTLTARVTLINALIMYQLVEAPLWGEVKARPCSYCFVPNIRAPTPRVENTNTLLPIGRDDKPCWNPNPPCGESIPVILFYCNACYTTAYAPLRMCSKAHSLHEVYVNNSRREIIEATLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYE0</accession>
    <name>A0A5B0QYE0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">FRS1_2</name>
        <name evidence="17" type="ORF">PGT21_031930</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYE0"/>
    <dbReference type="OrthoDB" id="1698572at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000004">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000023">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="16">
        <location>
            <begin position="297"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1118119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="635" mass="72214" checksum="563786741EB31F62" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZH3</accession>
    <name>A0A5B0QZH3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002710</name>
        <name evidence="3" type="ORF">PGTUg99_004263</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000039">
        <property type="protein sequence ID" value="KAA1135295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZH3"/>
    <dbReference type="OrthoDB" id="10622086at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="182" mass="19417" checksum="04290FFC8FE1E7A4" modified="2019-11-13" version="1">MAMLVSEDSPEDESELLSKDSAPPSAQHPPKLASEESLSSDSEDSSTSCSDATSPKNPPPPQPSSIPPNRDGRAGLYRKGLVAGRRKTLDVFRPLDFSSKTHQSIGHFPLSTDLTKQAGHSNKDSSSSSDEDGSESDLSRTDGKPLNTKKKKAENQTLDLPAHKLAGSAAFSKPTFLPPLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R142</accession>
    <name>A0A5B0R142_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_020064</name>
        <name evidence="3" type="ORF">PGTUg99_018186</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000512">
        <property type="protein sequence ID" value="KAA1065181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R142"/>
    <dbReference type="OrthoDB" id="2511173at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366584" description="Secreted protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="8341" checksum="27F595E0FFD7782D" modified="2019-11-13" version="1">MQLLQLSIFTLAAVLMASLHAEGVTSEIGSAERKLVQAEESIGSTAPSTHNSHEKRGLEKRKKKKGGGGGRGGGAKKKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R287</accession>
    <name>A0A5B0R287_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R287"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF2">
        <property type="entry name" value="CHITIN SYNTHASE REGULATORY FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="561"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="561"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="594"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="610"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="761" mass="83799" checksum="B163435DAD2E94F7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2W9</accession>
    <name>A0A5B0R2W9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">vesicle-fusing ATPase</fullName>
            <ecNumber evidence="3">3.6.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">VPS4_4</name>
        <name evidence="16" type="synonym">VPS4_2</name>
        <name evidence="17" type="ORF">PGT21_035981</name>
        <name evidence="16" type="ORF">PGTUg99_012943</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">21-0</strain>
            <strain evidence="16 19">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2W9"/>
    <dbReference type="OrthoDB" id="29072at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19521">
        <property type="entry name" value="RecA-like_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000004">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000043">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000015">
        <property type="entry name" value="vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF83">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIT" evidence="15">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="164"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="70"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1109510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1119897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="441" mass="48791" checksum="5EBE6526893B5E5E" modified="2019-11-13" version="1">MSGQSNFLDRACEIVRQAIDEDIKQNWEESLKLYKNALDYFHMAYKYEKNPKLKDLIRSKMEEYLDRAEKLKTHVQTPDDKRGRQAVGANGKGTNGEGGSGANADGDDADTKKLRGALSSAILAETPNVSWDDVAGLEGAKESLKEAVILPIKFPHLFTGKRTPWRGILMYGPPGTGKSYLAKAVATEAKSTFFSVSSSDLVSKWMGESERLVKQLFTMARENKPAIIFIDEVDSLCGTRGEGESEAARRIKTEFLVQMNGVGNDAEGVLVLGATNIPWQLDIAIQRRFEKRIFIPLPDPEARKRIFELNVGTTPCTLTQQDYRELASQSQGYSGSDIAVVVRDALMQPVRKVLSATHFRPVMVPSAEDPTISVKKLTPCSPGDPLAIEKSWNEVEATELLEPALTLPDFLRALRNTSPTVREEDIKRHLEFTNESGADGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3C5</accession>
    <name>A0A5B0R3C5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RIC1 C-terminal alpha solenoid region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_037063</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3C5"/>
    <dbReference type="OrthoDB" id="67540at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034066">
        <property type="term" value="C:Ric1-Rgp1 guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040096">
        <property type="entry name" value="Ric1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009771">
        <property type="entry name" value="RIC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746:SF10">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR SUBUNIT RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746">
        <property type="entry name" value="RAB6A-GEF COMPLEX PARTNER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25440">
        <property type="entry name" value="Beta-prop_RIC1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07064">
        <property type="entry name" value="RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIC1 C-terminal alpha solenoid region" evidence="4">
        <location>
            <begin position="858"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="908"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1120048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1119" mass="125467" checksum="7CAE6FE3E6CE7C13" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R460</accession>
    <name>A0A5B0R460_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_037115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R460"/>
    <dbReference type="OrthoDB" id="2506864at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="587" mass="66735" checksum="D0618E265E525B41" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RAC9</accession>
    <name>A0A5B0RAC9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018665</name>
        <name evidence="4" type="ORF">PGTUg99_028446</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000237">
        <property type="protein sequence ID" value="KAA1121774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RAC9"/>
    <dbReference type="OrthoDB" id="10577663at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138082" evidence="2">
        <location>
            <begin position="22"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1121774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="181" mass="20582" checksum="40810EDFDFCDDD00" modified="2019-11-13" version="1">MNTIFQSLLISTIVATTWVNAVLLEVTHNNHDYYLCSVDHEFPRDVDDNPFCLLGEVDGEPHDNARVRTIKNTSTNPIWIGSGFPAHSADYQLDPDQERERSFGRTDRLLQEHLGHYVIVTLEEGAEISLPTWEAILKENHENLKNAVINQGKLKDARLDTPHSQPFKRLSESEQAPEPTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RG76</accession>
    <name>A0A5B0RG76_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009788</name>
        <name evidence="3" type="ORF">PGTUg99_036435</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RG76"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="56" mass="5996" checksum="53178B679B14F29B" modified="2019-11-13" version="1">MAKVGAQLFNLNSPSPQAFGLRNPADHPIQTELIQVFSINSGLSTPTTSSKKNNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RK91</accession>
    <name>A0A5B0RK91_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sphingolipid homeostasis protein orm1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_016176</name>
        <name evidence="7" type="ORF">PGTUg99_025942</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000173">
        <property type="protein sequence ID" value="KAA1126127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RK91"/>
    <dbReference type="OrthoDB" id="1932233at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007203">
        <property type="entry name" value="ORMDL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12665">
        <property type="entry name" value="ORMDL PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04061">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="271"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="295"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1097651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1126127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="36291" checksum="34FEB3A0310CB4EA" modified="2019-11-13" version="1">MPTITTSASPEISLSPPPPDSPISRKSNLRARVTFTATTTNDHNNNNHKHSAPPGSLISPASSLGFSSHSNPTSPLQSPSNFSDSDEFKHGRGRSSSLLSVHEVQDNYDDQLDQGVGPNLNVDWVDYKGAWMIHLVLIFSGKILVDIIPGITQDVSWTTVNICYDVVTYIMFHYITGTPFESSGGVYDRLTMWEQIDMGAHYTPTKKWLTILPIALFLLCTHYTHLDLHPSLFTLNFTCTFCMALLPKLPIFHRLRFRFLDGVIGWSNSINDERSGPPTPIEEQMRRMPEPDFAYSSSGLSPRTANTTFIGRPPSPLSVNRGDPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RQM9</accession>
    <name>A0A5B0RQM9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008835</name>
        <name evidence="2" type="ORF">PGTUg99_019386</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000143">
        <property type="protein sequence ID" value="KAA1128266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RQM9"/>
    <dbReference type="OrthoDB" id="2496429at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1128266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="436" mass="50133" checksum="DF4A1ED2F0B817A3" modified="2019-11-13" version="1">MNNAPLWIIEAMVSQGFESFPGTCRRKISAIDGADYPGMRRAPIQQIKARKKHLSEIRKGFLPTLRQLLADLIVAQDLVDPQKEPKYPDVREINRITCRIVDMLREIRYAASTIALTEPQAPEDEDHEYGILKRYRTKALVEKLRYMIRYPLRESLGYHGDFFEQWKNSKERVVKRRFDYPGLLIATATAFHFIDEIIEWLKKSDFGVLQETWKAALDGYEDRMTALLNRLNPTIRSEHERGPASAHSETRLSQSEANNLVGHEEDQLGNFVASDTSPVLPRPRARLLKLLLSVLLLGKLARILFNKLLITPISRPGFTLDSQMSSAQIDYLTNQTRSVFFSIWNLADMLATLYDLDRLENLEAAQHDLDKILGYFDSSIRLLSLHLVPSHPNLQPGLPASENIFKHRFSLLIDQFHLAAQNLTNDLKRFTDLDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SDJ9</accession>
    <name>A0A5B0SDJ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018214</name>
        <name evidence="3" type="ORF">PGTUg99_033767</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SDJ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1136156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="55" mass="6982" checksum="F57332D29C56E8CA" modified="2019-11-13" version="1">MFLIKQTKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKIMILLNYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LHV6</accession>
    <name>A0A5B0LHV6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008243</name>
        <name evidence="2" type="ORF">PGTUg99_008953</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000518">
        <property type="protein sequence ID" value="KAA1063949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LHV6"/>
    <dbReference type="OrthoDB" id="10255632at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051307">
        <property type="term" value="P:meiotic chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005314">
        <property type="entry name" value="Peptidase_C50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792">
        <property type="entry name" value="EXTRA SPINDLE POLES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792:SF0">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="467"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1063949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="629" mass="69597" checksum="A5B4F79688CA35EF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LIU5</accession>
    <name>A0A5B0LIU5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Nascent polypeptide-associated complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EGD1_1</name>
        <name evidence="17" type="synonym">EGD1_2</name>
        <name evidence="18" type="synonym">EGD1_3</name>
        <name evidence="19" type="synonym">EGD1_4</name>
        <name evidence="16" type="ORF">PGT21_024871</name>
        <name evidence="17" type="ORF">PGT21_026677</name>
        <name evidence="15" type="ORF">PGTUg99_000707</name>
        <name evidence="18" type="ORF">PGTUg99_022577</name>
        <name evidence="19" type="ORF">PGTUg99_026282</name>
    </gene>
    <organism evidence="15 21">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="15 21">Ug99</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Part of the nascent polypeptide-associated complex (NAC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the NAC-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000521">
        <property type="protein sequence ID" value="KAA1063803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000277">
        <property type="protein sequence ID" value="KAA1112518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LIU5"/>
    <dbReference type="OMA" id="AGDTYME"/>
    <dbReference type="OrthoDB" id="8033832at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22055">
        <property type="entry name" value="NAC_BTF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000003">
        <property type="entry name" value="Nascent polypeptide-associated complex subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039370">
        <property type="entry name" value="BTF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10351">
        <property type="entry name" value="TRANSCRIPTION FACTOR BTF3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="14">
        <location>
            <begin position="39"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1063803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1065095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1085990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1112518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1113836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="179" mass="19089" checksum="530D1439A335DCFA" modified="2019-11-13" version="1">MNNEKLKQLQAQTKVGSKGQPRRKVIKKPKGFTANGGPAGDDKKLQAALKKLNVQPMIGMEEVNMFKDEGSKILHFSNPKVHGAANVNTFAIHGNGQEKDLTELVPGILPQLGAESIANLRRLASSLGDLHQANSMSYKPPNMAAGANNDDDDVPALVDSFDVDEGAAPAETTAFESVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLP3</accession>
    <name>A0A5B0LLP3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLP3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022763690" evidence="1">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="100" mass="10931" checksum="F054C4813C79375A" modified="2019-11-13" version="1">MAPFNWSINATLMAAILLIFLSSPIQAANVERRQGQDQCNPANDHLTIHDCAVDRVDSISSAFYNLGFQGKNKILRGEGPVNHAFQSCRVIVDCRGGTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPU5</accession>
    <name>A0A5B0LPU5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPU5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="52" mass="6033" checksum="95FDA882D50DD98D" modified="2019-11-13" version="1">MPTHLQASFRNREETSKPLAKLNNTLWQHITPAKKAYKNCLATGRWQRPLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRS9</accession>
    <name>A0A5B0LRS9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_013644</name>
        <name evidence="3" type="ORF">PGTUg99_019705</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRS9"/>
    <dbReference type="OrthoDB" id="447103at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="21"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="635"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="645"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="840"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="858"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1071640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="873" mass="93322" checksum="402F021FAEF85B66" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LW21</accession>
    <name>A0A5B0LW21_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018580</name>
        <name evidence="2" type="ORF">PGTUg99_034298</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LW21"/>
    <dbReference type="OrthoDB" id="2139939at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044688">
        <property type="entry name" value="SCI-1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34117">
        <property type="entry name" value="STYLE CELL-CYCLE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34117:SF1">
        <property type="entry name" value="STYLE CELL-CYCLE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="335" mass="37730" checksum="2FDB435B6C6FE9E0" modified="2019-11-13" version="1">MTRTTSQNPGKKRSRSRSSSAADSAHESPPRSRHRRRGSNPITSGLPPPGVQPISKDDFFLKSLEFKVWLKNDKNKFIDQLDSHKSKKYFEKFVRYWNKGKLDEDYYNPPAHWRTRSSAATSSHSWTFKGATTLDREKARQALQEASIGPSLPGGDKGPGASKPAIIGPSLPSHLLQPSSSSTPTITTLAEHQYKVDQELDGEAQEQILRKNEAKRKRKEDKEEESANRATGKARLLEKRKEKRDAQGEYLRQRDDPTGPELDDRSLFGSNNSFQEAIRARDRAKERRTGKNASFKVEKQLVMQEKVAAMKSKEDETMAMFKALAASKFGPSGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXA6</accession>
    <name>A0A5B0LXA6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXA6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="96" mass="9931" checksum="76A96E5B24FD5C82" modified="2019-11-13" version="1">MAPSGQGGSDKVLMTPAQRWPLVLGGITPLAPGRHDRPCPSTVTRVTPVRFQATTPTLCASPSFGGSLREGRLRQPGTGPPIEGLVVKLVPGHNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXW9</accession>
    <name>A0A5B0LXW9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Glutathione S-transferase 3, mitochondrial</fullName>
            <ecNumber evidence="15">4.4.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Glutathione peroxidase MGST3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">LTC4 synthase MGST3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PGT21_025152</name>
        <name evidence="22" type="ORF">PGTUg99_021329</name>
    </gene>
    <organism evidence="22 25">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 24 25" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">21-0</strain>
            <strain evidence="22 25">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>15-deoxy-Delta(12,14)-prostaglandin J2 + glutathione = 15-deoxy-Delta(12,14)-prostaglandin J2-S-(R)-glutathione</text>
            <dbReference type="Rhea" id="RHEA:75963"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:85236"/>
            <dbReference type="ChEBI" id="CHEBI:194498"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:75964"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>leukotriene C4 = leukotriene A4 + glutathione</text>
            <dbReference type="Rhea" id="RHEA:17617"/>
            <dbReference type="ChEBI" id="CHEBI:57463"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="EC" id="4.4.1.20"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:17619"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="14">Lipid metabolism; arachidonate metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Lipid metabolism; leukotriene C4 biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.20" evidence="15"/>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXW9"/>
    <dbReference type="OrthoDB" id="410651at2759"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:glutathione transferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:leukotriene-C4 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:lipid metabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
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            <begin position="12"/>
            <end position="33"/>
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            <begin position="125"/>
            <end position="147"/>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037916"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039056"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069748"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00075145"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00076908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAA1068434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAA1104498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="150" mass="16514" checksum="9A131E945DBCD662" modified="2019-11-13" version="1">MSPLIFSFPQDYGYVTLAAASTGFLGLFQTMAVGRARKAAKVPYPISYVSHEEALGDPAKMKFNCAQRAHANTLESLPFFLFSLLFTGLRHPRFSASCGALWVVGRVFYTLGYNTGDPAKRSRGVFHNFGTLGLLLGSTWVSLQMVMETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LY78</accession>
    <name>A0A5B0LY78_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="2" type="ORF">PGT21_022169</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="32"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="166" mass="19168" checksum="DB9D07CA4B34BB26" modified="2019-11-13" version="1">MTILRINQVKSHQMRPKVTRFFVAKFSRDQRDANHISGPPILQDANPPSENETQNGRKKRRRSSYVDKKALNRYWPTHPAVVRPLNPAASPVRHPRALTLILHEGPPSRLRLGTPPRPHEQGRLGNPDENDSINQIQDDDDLNNRSRDDSQDEDEESRPRSRRRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYG9</accession>
    <name>A0A5B0LYG9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1069451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="23"/>
        </location>
    </feature>
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        <location>
            <begin position="40"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="468"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="668" mass="73315" checksum="A75C7F2394E71170" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYH6</accession>
    <name>A0A5B0LYH6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYH6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="82" mass="8889" checksum="976D3982D1BCC331" modified="2019-11-13" version="1">MTYGYGYGYGFTLAYHNPLVSGAGGPARRGRLAKRASERGAWEVGCPARLCGSRFQGILIRILTKSLISSLDHLLREGGVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M052</accession>
    <name>A0A5B0M052_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_002423</name>
        <name evidence="10" type="ORF">PGTUg99_037265</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M052"/>
    <dbReference type="OMA" id="KKPIREY"/>
    <dbReference type="OrthoDB" id="78296at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098771">
        <property type="term" value="P:inorganic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000005">
        <property type="entry name" value="Putative Cation diffusion facilitator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF4">
        <property type="entry name" value="CDF DIVALENT METAL CATION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="8">
        <location>
            <begin position="171"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="110"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1070182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1090221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="456" mass="51505" checksum="67FC7F5C8FC96FEB" modified="2019-11-13" version="1">MTAGKPINERTALLRHPLPQILKHHSSPAGELLPQTPSTQPPLISHSNFHLTNAYHLAGLVNDQGGEYEKYRKSAEELKQLSSKARQFYERQNEILDYFQEIDSVLDSSRRRSSAGVTPERRRSEGLIEPHDLEADPLLDHEQRPGEPVECAHKLDRERKESLSVRRTISINFFINFLLLAGKIVVALLSNSISLIASLVDSAMDFLSTAIIWWTNRKIDSKSWQSIWQYPAGKRRMEPMGVVVFSVFMISSFVQVLVESIERLFAGSNAGISIPFTSMIVMWVTILVKGVVWLWCRRKKNTSVKALAQDAENDCILNIFSLLFPYIGLKFNLPWLDAVGGLILSLYIITEWTGTLFDNVKNLTGRRADPIQHQRVAYLVTRFSPLIQAVQHCHVYQAGDDLIVETDVILSPETPLPVAHDVGESVQYALESLDGISRAYCHVDFSSNPMSGHFQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M401</accession>
    <name>A0A5B0M401_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M401"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="105" mass="11468" checksum="4C15E9513DD07E71" modified="2019-11-13" version="1">MGLINLKPIAPILARDSSQLTGTQSGPNIQLNLGLIRLPIEKKTLGRSGIHRGRLIGRDSSLVLSISMIDPSTLPSTSIRAEKNALSIPVMSFVHFRSVRCYLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M4C8</accession>
    <name>A0A5B0M4C8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020122</name>
        <name evidence="3" type="ORF">PGT21_020245</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M4C8"/>
    <dbReference type="OrthoDB" id="10628157at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366085" evidence="1">
        <location>
            <begin position="22"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="121" mass="13168" checksum="0695DE42EEF3C6BC" modified="2019-11-13" version="1">MYHHALHHVSLALHHVTPCFGECKPGFTPCKPSFTPCKPSFTPCKTGFTPCKTGFTPCKPGFTTCKPSFTPCKTGFPPCKPGFTPFNTMLPLHHPGFTPCKPGFTPCKPGFAPCNTMPYSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5J9</accession>
    <name>A0A5B0M5J9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019987</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5J9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="66" mass="7562" checksum="98698F33BDE3ACDE" modified="2019-11-13" version="1">MVRLNDVCDISSKNSQYIPEETPYFYKKGMAKLSSDSDSGRINRLRPPPISNWDVDEFATGFVAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M684</accession>
    <name>A0A5B0M684_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_009253</name>
        <name evidence="4" type="ORF">PGTUg99_031326</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M684"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366099" evidence="3">
        <location>
            <begin position="24"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1067290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1071480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="192" mass="22185" checksum="34766205320BADE2" modified="2019-11-13" version="1">MMFKFINMFTWLSIFSLAGKSFGTTSMPELRPVTCTTGKQSEIIGRHPFSENEHSLPHHPHKDNEDSLPHQTQPLFSPTNSMEHIVKIEDPVPEDEKMVIDMREDDRSIEDGQQERDRPTETPTNNLSSHAAPQNQNRINMALDLKHPIVRYLRENWDWWGGIAAGIIISVHFVNYFAKIVMEEKMNYGPNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7V1</accession>
    <name>A0A5B0M7V1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004626</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7V1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="207" mass="23536" checksum="3EDA203050800C39" modified="2019-11-13" version="1">MYLVQRKALHTSTSKHQDPSTLVAAKDFDIYNMYRDQILNAHLPNFQYHDKIPVFIDPGDQSHYILLTTGNVDKGAHDLTKPNTAVSLHFPPPKLKFEKLSASKKCKLDHCSSCCTRRITSQSDSNESSVFDPGVNLLEAYLEFIKIPAIERSEIARILTEHKAKNPIFFQSKNITRDVMKGWGLAGIYIARLRDNVKKFEKSKASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M894</accession>
    <name>A0A5B0M894_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034209</name>
        <name evidence="3" type="ORF">PGTUg99_013224</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000245">
        <property type="protein sequence ID" value="KAA1120321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M894"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="158" mass="16197" checksum="BFB50D7FBEF61AB6" modified="2019-11-13" version="1">MDPSFVTPSPLSQQSIDSPSNTLTTGPDVACLTLASNFGDSPQPLTKGAGGTKKQYCQTQKEMAAYRAKQAQLKKAKALAKAKGKRSKACGGGSQSSRCGGESAPGPKLWPVCLGACGMRAADAESMRSCKKNKCGDLSGSLSGADQALGGQFLSFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8Y2</accession>
    <name>A0A5B0M8Y2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_035341</name>
        <name evidence="2" type="ORF">PGTUg99_025131</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1073071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8Y2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="83" mass="9145" checksum="FA2CA0C66E79D469" modified="2019-11-13" version="1">MHNISFPELPPSSLPIALEDAFALAIFGYIIFYIIRKICTCQSSQSTNTNIEKAATFRLSHLTKTDPDMAIIVLVALGIYAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCJ3</accession>
    <name>A0A5B0MCJ3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCJ3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="194" mass="22320" checksum="000AFCA467059028" modified="2019-11-13" version="1">MGKPWPQAELEPAAALSGFRGINFFVSSCDKANLTRKSAVAHRGNIQHFTETRLAFKFLNFPGEKGRERLHSQKNLINMEYPFRLAFVFLLAITLMCRSTTQVMNSHVVAYPDRTNSLRLYTDDSEPRDAIFCYIPEGRTQWRVRNHGDRLVHLRAMRGCSEIDIFRLEPNHEHDVRTVPSTENLNLQIISTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCN3</accession>
    <name>A0A5B0MCN3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_012289</name>
        <name evidence="3" type="ORF">PGTUg99_005868</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCN3"/>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="41"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1074049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="87" mass="9898" checksum="79C4CFA5FE3E02A8" modified="2019-11-13" version="1">MFVMEKALNSKPSGPPPLHASSGFLVAQRVTLCLHRQSIRPALYLQPDDSPGRQRTLTCHWESNPSYQDVRWTKPDRSKPGVLTTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDX3</accession>
    <name>A0A5B0MDX3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_007030</name>
        <name evidence="10" type="ORF">PGTUg99_028050</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDX3"/>
    <dbReference type="OrthoDB" id="406631at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000251">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF39">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="9">
        <location>
            <begin position="153"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1074170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1090614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="539" mass="60932" checksum="F45ED59EDC9FCDD1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEI9</accession>
    <name>A0A5B0MEI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Bud site selection protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BUD20_1</name>
        <name evidence="13" type="synonym">BUD20_2</name>
        <name evidence="14" type="ORF">PGT21_033562</name>
        <name evidence="13" type="ORF">PGTUg99_024394</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="13 16">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ZNF593/BUD20 C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000476">
        <property type="protein sequence ID" value="KAA1072880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEI9"/>
    <dbReference type="OMA" id="MKDHFRS"/>
    <dbReference type="OrthoDB" id="24683at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000299">
        <property type="entry name" value="Zinc finger protein 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051879">
        <property type="entry name" value="C2H2-ZF_Maturation_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1072880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1075315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="146" mass="16267" checksum="BBDDC766ABDADE18" modified="2019-11-13" version="1">MGRLRRSRTHSSAKSQIKSRKTKRYTRDLDQIAIDMELAQSIRSVKQAQSDSQSNFVVPQAFGSTSHPTVTGEVDEDTTGCGQFPCVQCARFFVDSRSLTEHQRGKVHKRRVKELAAGPYSLEEAMRAAGIGTDNRQRPAPDLMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFI8</accession>
    <name>A0A5B0MFI8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030640</name>
        <name evidence="2" type="ORF">PGTUg99_030329</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFI8"/>
    <dbReference type="OrthoDB" id="10506654at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137222" evidence="1">
        <location>
            <begin position="23"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="128" mass="13997" checksum="7A71754D78B1FAD6" modified="2019-11-13" version="1">MQLLKSVFIGSFASQLLVLTASSPGPTTPIIDPGTTFTCFDAKKGFNIGWCATLHDVGEGNDISKIYSYSVTKANQVGAKAHYNYNCNGKNDQSKKCCRADWKQKKDEYGYVIITGDDCQDKPAVKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFK9</accession>
    <name>A0A5B0MFK9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000151">
        <property type="protein sequence ID" value="KAA1075725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFK9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="343" mass="39797" checksum="424CB4BB873AF0AB" modified="2019-11-13" version="1">MEAPSREQKESDKLTLDPSTLKSQQHPIQADMDEYERFATLHGYQENQQAEHFQVVLKAVGVERLQNYHDDLEEILSKLKPPDTKQEDVISRWANFFQGYKRSFAKLKKILNLTDAIMRIKSSNLSSSLTSFDSPPRKKTLECRKKRCLPESKLFTSQTANALGSFTASRELYLSNRSQCFSRLLELRVYLTKLENIVELKPPDTKHEDVISQWAKFFQGYEGSFARLKEILKLTDADHESQIKQLVELAHFIRFPMEKEPSERLLEEMLTRIQQSDKKSRKSQQQSTAFKRFEKLHGYGKDEVAGKFIAIVEAAGVARLQKYHEELQSLMHSFNDDLSACRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MG74</accession>
    <name>A0A5B0MG74_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MG74"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="119" mass="13277" checksum="07CEAA959CC154AF" modified="2019-11-13" version="1">MIVTIAIATLYRDILEWEAAVDAGGSGFRSRFFGPRSVPEGRLWDVQHDYNTAPRYSPPTPYSSRAIQDDRSLISSSEVAQSSQRRRGRLQTFFAAPLFNVANLPFVDLFLSLATGAHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML28</accession>
    <name>A0A5B0ML28_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_003373</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COA8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML28"/>
    <dbReference type="OrthoDB" id="6246201at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018796">
        <property type="entry name" value="COA8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31107">
        <property type="entry name" value="APOPTOGENIC PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31107:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10231">
        <property type="entry name" value="COA8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1077332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="230" mass="26688" checksum="EF28975B08A75DC7" modified="2019-11-13" version="1">MLTRTVKPEWAFISKRVVRLYSTGPSTDAGGRRGRLTTRETLVDIGRGAAEDQVGPPCARSNLRPIRYSARFSSPTTSSPPTSLVESPHRHPYSLEEFRAEPVELSGRLAELRARLELEDLQWSMFRNRVDKLNQAFWETQSTRFEALEQKQRDAVLAQTVNLPPESVEERLNTRLDQFYASWLIDQKKLFMLYNWRWWGLQAALLKGGWLAQVRDLKWKLACWRDTIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML61</accession>
    <name>A0A5B0ML61_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML61"/>
    <dbReference type="OrthoDB" id="2495337at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="856" mass="98075" checksum="1BB1DF8B8A3C0857" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MLU2</accession>
    <name>A0A5B0MLU2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MLU2"/>
    <dbReference type="OrthoDB" id="2499555at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="450" mass="50938" checksum="30CDF97FE1F98257" modified="2019-11-13" version="1">MSMSIQPENVYTVQSLALHQLFWKAAKLNIPPFLPTLVWLEVFLEPIAGILYIISAIPSIRKEGFWLYKMETNGMIRPNTSTLIPIFVLLYITCSLSSLICLLIDMDSMTILSTRTVFLSLISYPILLCTGWTKIWNVLQAIPLTKYGLATMRQTNGDSSLKFFRPRTINLLSVFLYGFPVIFGSVTIGMLTNEVSKITQTFMEYNHGYSDIISGQIDNESIARLNYLAIQQLISMLHRSEKALLLSRLIGFGCFFNGLFLLSVMSFGYYRILKAVGYQIHTFRKASEHQAPLAFAVGTLQSSYKLESTPVPNSFNSTSSGSIRTQARRSPSLIWITTHLPTWLPSLRPDPEFLGKPDPASPNTHFDSQKWESLNQEMIHSQYKALKSNLLQVPVRYSLSDLIWVTITWASWSWILTVGLPFGVVACIVAFSPPITALRENEERVEEFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MM15</accession>
    <name>A0A5B0MM15_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MM15"/>
    <dbReference type="OrthoDB" id="10320857at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="440" mass="49459" checksum="F83CC96F24C62A8E" modified="2019-11-13" version="1">MIKSSHRLSRRVVATRETPEFASRGPEWVLKKPLLPPEPCFPIFIITYQHKPSPTPTLSTPLTHLSERLKNSPPSPEDLLGLEISTMELEKTAKLIQYYLTLLASEKAQRAADYQPSDWELLTPEEQRARALRRVEISENERVANLLLWNSVKRDHKNKRRQLLAELRNKRLAKMSATSNSSAATTPAAGNTPIAETAANSPNTGRTIQTSTAPYPQPLDRGAANQLEERVNRLKLITIQKKMAPVTVERPTEATLAENGSTPAVETPATARATQIVAKTTKNPAEKAADAMDIDPQASSFSADTQLRPKSPDVRVLSKEERIQLLVREHVALWKKYIADQPLGASEGNRALLTQAQDSQRALQKLIPRAEVEEYVKGWNPWTEKKNLFPVLQQERSGKNRSSSSRSNSYKAPKMNDPRRWKRLMKACHTLESGYMNMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MN73</accession>
    <name>A0A5B0MN73_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MN73"/>
    <dbReference type="OrthoDB" id="2504921at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="321" mass="35413" checksum="0498D2B0B0071C3D" modified="2019-11-13" version="1">MNHQSGSGGWKSKYGRLKGRHVKRTTNSSASDATEDSVETTELRECLASNLSGPACQNVTRIMEAPSSASSVLEANKTNNTNNTGALATASLAPNNTSNMVPSTSPPIGPNNATTTAAPPGAVARDNRLGWKIGLAAPLLVILLIIGLAFRHKRAKKLSMREKWQELSIPQEVVGEKRQEPEAESQKKTNAIQPRGNREESLQTEEILSAPARSKRKEPSHQEMLDLERGTPAISVRQGDEEDNRSRKSSVVSRFSLRSFSLRPAMDMSIFQARNDAPLDTFQLEIKHRERLSKAKYIDVLSDSRCIYIIRLQYLRPPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQR5</accession>
    <name>A0A5B0MQR5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005688</name>
        <name evidence="4" type="ORF">PGTUg99_031099</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000439">
        <property type="protein sequence ID" value="KAA1082277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQR5"/>
    <dbReference type="OrthoDB" id="418757at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="2">
        <location>
            <begin position="41"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1082277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="97" mass="10584" checksum="35BC4219A3CA94E4" modified="2019-11-13" version="1">MEKCRTKAQAKAEEDDSSTSSVAWHTIKKDFLVKLAPSVAYLDSGALRHMISDQSYFSTYSTNSECKIKLADGKTTLCPGIRNAGGASKQQLPKKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRC0</accession>
    <name>A0A5B0MRC0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013336</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRC0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="132" mass="13882" checksum="D65530B8A16CE870" modified="2019-11-13" version="1">MMIVVKGFFSLVKQFAGCKIVPAPAALACGLARQTPLVCRPARQPAGYIPAGHWRAAPHAIDGLACRRARQSAAGVRIGVREGGWLALINPRSSHASPVGATVLTFNYGLKTKKGGNQSSRLVSTLNSLIHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MX79</accession>
    <name>A0A5B0MX79_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromatin modification-related protein EAF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">EAF3</name>
        <name evidence="12" type="synonym">EAF3_1</name>
        <name evidence="11" type="ORF">PGT21_024434</name>
        <name evidence="12" type="ORF">PGTUg99_008993</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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        </subcellularLocation>
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    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="CBD_MSL3_like"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MRG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="MRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038217">
        <property type="entry name" value="MRG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026541">
        <property type="entry name" value="MRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053820">
        <property type="entry name" value="MSL3_chromo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880">
        <property type="entry name" value="MORTALITY FACTOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880:SF15">
        <property type="entry name" value="MSL COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MSL3_chromo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51640">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MSL3 chromodomain-like" evidence="10">
        <location>
            <begin position="85"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="MRG" evidence="9">
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            <begin position="198"/>
            <end position="367"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="385" mass="43872" checksum="E1ADE85670894456" modified="2019-11-13" version="1">MVYVRRVSPITASGTLRGSARSYVALHGSGTGLSPRNPLGRVRCRARGAAGQPTRLIISQTQLGGSQIRPTHRIIPPTNMPKMEFSASERVLCYHGPLLYEARVLKVKEAASDEARDIPKGNQYWIHYKGWKQKWDEWVSESRLLKLTEENLRLERSLNENHRVKEVPDKSTSKDAKNNDPRPTTNSDKGKKVEGRGTKRSRDSVCEPEEGPSKQPVTIVIPEPLKIQLVDDWEAVTRQNQVVSLPRTPTVKSLLEEYERYAVDDSTTPQAKNLIKEVNAGLKVYFDKSLGYCLLYRNERQQYIEIRKKLKGKLASEIYGAEHLLRLIVNLPEMISHTKMEPEIINIVREHVAKILEWLVTEQSRVIQSPYADTSTAYQKINRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N1N3</accession>
    <name>A0A5B0N1N3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029568</name>
        <name evidence="3" type="ORF">PGTUg99_032570</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <end position="55"/>
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    <sequence length="552" mass="59934" checksum="C30A9A82DD012D0C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N439</accession>
    <name>A0A5B0N439_PUCGR</name>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
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                <person name="Silverstein K.A.T."/>
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                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <end position="88"/>
        </location>
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            <end position="136"/>
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            <end position="73"/>
        </location>
    </feature>
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        <location>
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            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="512" mass="56780" checksum="0893983A7D1A88F5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4S7</accession>
    <name>A0A5B0N4S7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    <organism evidence="2 3">
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="31"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="173" mass="19059" checksum="51128FA84FEA1052" modified="2019-11-13" version="1">MAYPYTFLDGSGAFRADGWFDRLEENRFPPPSILTFGQAPGKSSGASLHPLTTSRSKLTPESHPEALRLLSQSQAVEAKPAPGLSHCIKPQKGFKRKKAAKKAADTINRSRHFPSTRLVPKTNQSTINSKSEQNQRPVSPKGQSSKPVRKRLLWIDNGSNEDVQKATPKWPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N584</accession>
    <name>A0A5B0N584_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N584"/>
    <dbReference type="OrthoDB" id="2506685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="597" mass="68687" checksum="BD3AD00DDD3D1A09" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5Y4</accession>
    <name>A0A5B0N5Y4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012317</name>
        <name evidence="2" type="ORF">PGTUg99_028806</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5Y4"/>
    <dbReference type="OrthoDB" id="10378317at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="218" mass="24089" checksum="6C9EAB0CD8C8B469" modified="2019-11-13" version="1">MSEVVVLREGISNLRTRLETLESRLTMIPSAPVQLSSQLSSCRLAKSKEKPVEGEEAISHVSVKHPRKSSSCTKTDSKTLRSRSFKVMRHLINVVDLSHVEDVASRPKKVQSNVYTSHDSTTFCLPATTNPKTTSRMPSRKAVAQLSHRKESLVLADSLHPTTDLQAPETKCTKKDSLRDPIASISPATKSKASTEPKTTISWPLKESTVRLTSRQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N626</accession>
    <name>A0A5B0N626_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="3">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_034721</name>
        <name evidence="8" type="ORF">PGTUg99_019597</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1073011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N626"/>
    <dbReference type="OrthoDB" id="9995526at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767:SF2">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE TRUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="TRNA-PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase II N-terminal" evidence="7">
        <location>
            <begin position="125"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="296"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="319"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="383"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1073011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1084717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="509" mass="56404" checksum="E34E7A977573FA47" modified="2019-11-13" version="1">MISVRHFSGRPPLSSLTGLTPDWTTTRSCNLAIPKDLGRRSFSKMGEPIGPAPSSSSIADFHKRPLSGLFAINKPSGTISMTLLESLKELFLSSKLFVDNPEPFQIEKTTGVKKSRKNKYWQKKNQAKAIKIGQGGTLDPLASGVLIIGLNSATKKLSNFLDCSKAYRTTGILGCKTDSYDSDGKTVGLAAWKHVKPEDIRRECGLIKGEHWQIPPIYSALKMDGKPLYEYARNNIPLPRAIEARRCEISEIRMVEWKEAGEHAYKWPTKMMSEEDSKIFQRAEHLVQQTGVVKGNNSTSLVVPPKQEAQEETNTTLHESSKRELPLEQEAEKESKKIKLEEVKEIKKGESDQPNDPTTPKLEEGNDPKIVKPEDGSSRVDDAQSSTNDPPIGQENTDGVTMEDNGKSPVFTLEMTVSSGTYVRTIVHDIGQAVSSAATVVSLIRTRQGDFCLDPPPKDPHGDDSKSLGCIEWSVFENAIQARSSGSDYPVDQAGFREWERELLKHIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7D1</accession>
    <name>A0A5B0N7D1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7D1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="410" mass="45818" checksum="499F9D3D6D12A561" modified="2019-11-13" version="1">MHRGRSPRAEDSSDDDEEEEEEEILRLLVPGYAALRPPPHPQQQQKSSPPLPSFYSTANRSSTGYPLKRLVKYRGGNFSSDRLPVYNGQMISTQAYLDFHSGHPFSSFRLPHQQHQHQHQHQHQHAKWKNGTRKNSLFNQQTTILSAVCSAGLHSGQPSLSTNSSDSPSSSTVSSPISCQPNHSPDSTTQELAVVPSIAHRITNNKKLDAGNHPGAYKPTKNPSKIIKQRWKDAALTFAAAQLNHPHHHRHSHRLLSSSPPPLPHDLDLPERLRRKEFGHKKKSGGSGGTSKAKYYVDNDQPAPSALHHHHPSSSFNRHNLNVALDYGDDGEDGLGGEPSNALLQDQDQDHHQKKKKKIMMIQMMMMMMMMTNDADTDLHFLRHPPAPRPPTTSIHTSSSSPSRPIRSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7X8</accession>
    <name>A0A5B0N7X8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006128</name>
        <name evidence="2" type="ORF">PGTUg99_001128</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000252">
        <property type="protein sequence ID" value="KAA1118369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7X8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="71" mass="7710" checksum="0C65B814D355CF69" modified="2019-11-13" version="1">MVFKEVCAGGNQGKGTMPTLKPADAKIELQKSTEGGENMRAQPFNGGTRSSQVTRISYMICNHHQDQVNQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8E4</accession>
    <name>A0A5B0N8E4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8E4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="12017" checksum="A190C3ED89B9F2AF" modified="2019-11-13" version="1">MVKGYPSRIPAIPWRIPARDSGYPPGDSDSGRGCIAQAAAAAPPVAVVGGQYASCIGRRHPSPTTPPSDLTKAVDPPWSDTIESVHRSRLGARIPLRASWNDDQRTHNPPPCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8F9</accession>
    <name>A0A5B0N8F9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000109">
        <property type="protein sequence ID" value="KAA1085016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8F9"/>
    <dbReference type="OrthoDB" id="2507849at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="707"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
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        <location>
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            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="440"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="611"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="709"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="732" mass="80851" checksum="624B352501B5534C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND46</accession>
    <name>A0A5B0ND46_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND46"/>
    <dbReference type="OrthoDB" id="10516872at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="160" mass="18800" checksum="8D0E41E52B9C7C53" modified="2019-11-13" version="1">MTSATLTIVLVIHNNASRKVAKKRCKAKKKQETRDQLLKRTLNPSDTTHFVNWRRVKYEELDLYPTIPVDRNKKPIRPPTPEEIKSAYNQVETFHLFKTGRNIVQDPLDKESVIAFIDFIKFEDMSEKDKADLNLFTTFLHRSKKFISPVATDVRLKKWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NE95</accession>
    <name>A0A5B0NE95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029882</name>
        <name evidence="2" type="ORF">PGTUg99_014942</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000414">
        <property type="protein sequence ID" value="KAA1086079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NE95"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="126" mass="13935" checksum="DB0DB4D482FF5570" modified="2019-11-13" version="1">MEYNTPTTASASAPPQYTLDPSPQSAVNPGVHQCPDSPQQAFRNLAHLDCLFPAFPRLGKLDFSTNARHFPQLAYVASALRAFRISRKHSAYAQTNRPKLWSRCALAATSVWVRSTFPKLLPEPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFP0</accession>
    <name>A0A5B0NFP0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFP0"/>
    <dbReference type="OrthoDB" id="2516998at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="137" mass="13535" checksum="EE5C2F577A967076" modified="2019-11-13" version="1">MLFGNLSQLLRLSILIVITISVSQISSFPQYLTSDPYGGGFNNGGIRGNGYYGGTGIGGGGLGGFGGGGLGLNSGFGGGLGGGLGGGFGGGLGGGFGNRGYGYYSDASSIQTPLNSTKLYWKSLLSLAVTGIFLLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLE7</accession>
    <name>A0A5B0NLE7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLE7"/>
    <dbReference type="OrthoDB" id="10285133at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="7"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="149" mass="16063" checksum="EB4E7A8623D823C3" modified="2019-11-13" version="1">MYLIFQASISEARSTAPSSPENARTTPVRLEALNVRIGTPIRISKRASVQGGDWNTSTTQQDTLPKVDGADSTSTQSEAHDTPIDTDHPSTKAHDPPKTSHPKHTSDGSRVHEYSQHDSKRRSTLSAGFRTTIPSVPAAVQEPLASGNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NR10</accession>
    <name>A0A5B0NR10_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Ubiquitin-like modifier-activating enzyme ATG7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Autophagy-related protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATG7_1</name>
        <name evidence="10" type="synonym">ATG7_2</name>
        <name evidence="11" type="ORF">PGT21_005819</name>
        <name evidence="10" type="ORF">PGTUg99_030037</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NR10"/>
    <dbReference type="OrthoDB" id="338614at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019778">
        <property type="term" value="F:Atg12 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019779">
        <property type="term" value="F:Atg8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01486">
        <property type="entry name" value="Apg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.70:FF:000001">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme atg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000243">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.100">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.70">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006285">
        <property type="entry name" value="Atg7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032197">
        <property type="entry name" value="Atg7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042522">
        <property type="entry name" value="Atg7_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042523">
        <property type="entry name" value="Atg7_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01381">
        <property type="entry name" value="E1_like_apg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16420">
        <property type="entry name" value="ATG7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like modifier-activating enzyme Atg7 N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="8">
        <location>
            <begin position="359"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606285-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1074204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1090580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="731" mass="82400" checksum="6CCCCA9AC809DC76" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NRC7</accession>
    <name>A0A5B0NRC7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_034228</name>
        <name evidence="3" type="ORF">PGTUg99_035705</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NRC7"/>
    <dbReference type="OrthoDB" id="2552042at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="582"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="638"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="803"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="654"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="757"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="820"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1076102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1091463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="830" mass="92956" checksum="AFB2E5B1600C87C3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NS55</accession>
    <name>A0A5B0NS55_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WDR59/RTC1-like RING zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_035005</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="311712at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16488">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="185"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="WDR59/RTC1-like RING zinc finger" evidence="5">
        <location>
            <begin position="1180"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="379"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="488"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="637"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="878"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="949"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="993"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1242"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="379"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="496"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="878"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="949"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="993"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1011"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1023"/>
            <end position="1033"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1091512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1293" mass="143611" checksum="1825CE90F2DC65EB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSV8</accession>
    <name>A0A5B0NSV8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Photolyase/cryptochrome alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_024094</name>
        <name evidence="11" type="ORF">PGTUg99_016874</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="6">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NSV8"/>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:response to stress"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Deoxyribodipyrimidine photo-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF18">
        <property type="entry name" value="SI:CH1073-390K14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="9">
        <location>
            <begin position="158"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="78"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="400"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="440"/>
            <end position="444"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="487"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="490"/>
            <end position="497"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="588"/>
            <end position="590"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="7">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="7">
        <location>
            <position position="575"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="7">
        <location>
            <position position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1084310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1092301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="690" mass="78605" checksum="5FF9D664E89E46AB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUI0</accession>
    <name>A0A5B0NUI0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091488.1"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUI0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="90" mass="10536" checksum="955B784451243A91" modified="2019-11-13" version="1">MVPSPSSHQLPPFDRSFERRCGKPILNLKAQQDYISHKNHRPKTPQEFLQALKQRQQTNNPHKYPHMDNNNSHTSETPQMKPKAKFPVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P078</accession>
    <name>A0A5B0P078_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P078"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="115" mass="13624" checksum="145067776D78AFA7" modified="2019-11-13" version="1">MSLLKRLQQLNSSLDNISLVVCPEISFLCFYTTTLISIMRRQWIFGPHCDQKQISRKLRDFDYIFWVAGKFDTKLGDNYFESFFEGKKNFKSLLDKCLRIWGLGRIGDKREDQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1H9</accession>
    <name>A0A5B0P1H9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar membrane-associated protein IML1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Vacuolar membrane-associated protein iml1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">IML1_2</name>
        <name evidence="8" type="ORF">PGT21_034405</name>
        <name evidence="7" type="ORF">PGTUg99_021220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IML1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000511">
        <property type="protein sequence ID" value="KAA1065304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="39497at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04371">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045838">
        <property type="entry name" value="DEPDC5_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027244">
        <property type="entry name" value="IML1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048255">
        <property type="entry name" value="IML1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179">
        <property type="entry name" value="DEP DOMAIN CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179:SF8">
        <property type="entry name" value="GATOR COMPLEX PROTEIN DEPDC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19418">
        <property type="entry name" value="DEPDC5_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12257">
        <property type="entry name" value="IML1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="6">
        <location>
            <begin position="1144"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="605"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="682"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1095"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1228"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="623"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="718"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="753"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1101"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1243"/>
            <end position="1254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1065304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1095003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1518" mass="169074" checksum="00497615ED6B5BD3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P494</accession>
    <name>A0A5B0P494_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000107</name>
        <name evidence="2" type="ORF">PGT21_012195</name>
        <name evidence="3" type="ORF">PGTUg99_011724</name>
        <name evidence="4" type="ORF">PGTUg99_037309</name>
    </gene>
    <organism evidence="1 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000077">
        <property type="protein sequence ID" value="KAA1095524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000249">
        <property type="protein sequence ID" value="KAA1118504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P494"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1095524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1132126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="76" mass="8672" checksum="13B3068F6832555B" modified="2019-11-13" version="1">MEEDWDDGGNYSSSPIDDLIENAERCYVQYESRLLTYKMHGGKWELVGTVPSRLAIGTADSGLQSRYRLLDKKVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5H6</accession>
    <name>A0A5B0P5H6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003275</name>
        <name evidence="3" type="ORF">PGTUg99_006809</name>
        <name evidence="2" type="ORF">PGTUg99_007171</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000045">
        <property type="protein sequence ID" value="KAA1134776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000011">
        <property type="protein sequence ID" value="KAA1137159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5H6"/>
    <dbReference type="OrthoDB" id="10258445at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090324">
        <property type="term" value="P:negative regulation of oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052000">
        <property type="entry name" value="ETFRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024:SF0">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN REGULATORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE SOLUBLE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1134776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="98" mass="11477" checksum="7DD2C5EA806063F2" modified="2019-11-13" version="1">MGTPRILRHQAVKVYKQLIRLSADYPDPNYDLAGRTRECFRETTGRLEGIEEGAVREEEMAKSIAKAIFIRKEVEALIFLSRYRELKRRYGDTRNSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P6T0</accession>
    <name>A0A5B0P6T0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P6T0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="347" mass="38855" checksum="10056AFEA57D7B29" modified="2019-11-13" version="1">MQHDRQESGHTTSNSIGSSNSLIPTSPGMITSNVIPSSGIYHSRMESFEGFSGQPTPPLVSSQPSRRPAFSTQSSSSHLSPSYPHQGIDQLNSSPSVRPFASLPRNHQMGSTVEFGTIDAKLDNYQFDFPTLQASSYGHEKSCGQTQRTSTEERRVKVDDRPIDIFDPEFLLHIPNLLGHPGSPSSQPPEQLSLNYTNLEAFQPTFNLDFGLTSQGALSGADLAGPADPRAFSLNPQYDSRSAIKQSNSVGYHEISNRNIEFNHHHQQQQQQQKQQQQQQQHHHHHQQQQHQQQQQHATNNVPITTASAGSHHQHPQHQQHSHPHYHQSHIAYGHEQPRYDNFNRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAJ7</accession>
    <name>A0A5B0PAJ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013963</name>
        <name evidence="2" type="ORF">PGTUg99_009927</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1133995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAJ7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1133995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="63" mass="7498" checksum="1B12C6BB8E003EE2" modified="2019-11-13" version="1">MRTVWSTKHRKYIMWASSTEIEASLLKSTPKKIQDAVFRLKCILLAPDILLNRWIFTKLFDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCV0</accession>
    <name>A0A5B0PCV0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029566</name>
        <name evidence="4" type="ORF">PGTUg99_028340</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCV0"/>
    <dbReference type="OrthoDB" id="2512080at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137747" description="Hydrophobin" evidence="2">
        <location>
            <begin position="25"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1116877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="17027" checksum="C9F9F5DE7BA5BCF8" modified="2019-11-13" version="1">MFYPGFHKCLVLAIFMGTISQIVADTKTPQPQPKPGPGPTKPGPGTTRTQPAPAPNSKTPPCEKGTTPVCVAAITDKISTEKHNPTTPGGSNPQCTSGTKICCSPAVLKHISSVKNKSKNKKPDDTVFSSLCALPGKALPAGAPKGAAAAAVTAKAPAAKQPAGKPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEX1</accession>
    <name>A0A5B0PEX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEX1"/>
    <dbReference type="OrthoDB" id="2510764at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="5"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="754" mass="84749" checksum="3FF497C965D666FE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFE4</accession>
    <name>A0A5B0PFE4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021213</name>
        <name evidence="1" type="ORF">PGTUg99_032755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFE4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1095471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="78" mass="8597" checksum="693548F10A9AE9C9" modified="2019-11-13" version="1">MRLEVQRGGPFSNNAPVFHPPPPPVWLLSTPTNDPMCKLCRNFYQLGWLTGTNGGSQLTTGSRLHRAIWCAEGAFEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PG43</accession>
    <name>A0A5B0PG43_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000058">
        <property type="protein sequence ID" value="KAA1098809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PG43"/>
    <dbReference type="OrthoDB" id="2499552at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="80" mass="9150" checksum="31747ADBED694228" modified="2019-11-13" version="1" fragment="single">EICTKIIRGILDSAQHQLSTSSRSVQSHPAATSQIWPKNLRVEDDWKGRGKWGESRPDHLEWLGCPKEAQELMLSLRAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJU5</accession>
    <name>A0A5B0PJU5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032719</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJU5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="66" mass="7784" checksum="0216325560775CF4" modified="2019-11-13" version="1">MLDVEQPLSAFEPQDTEDEHYLRTRNFRLNPRKDIYQGAQPILRFISKSQVIQLQCQRRCATTAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJV4</accession>
    <name>A0A5B0PJV4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015937</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJV4"/>
    <dbReference type="OrthoDB" id="2496057at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF1">
        <property type="entry name" value="DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="2">
        <location>
            <begin position="124"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="327" mass="36350" checksum="F0427CF0F81F75AD" modified="2019-11-13" version="1">MDFKKQHAPPPPPPPPPLNIPPKLCPSKSPAISAPPHPPEPPLLVKATFSSSAPAPPPPPPPPPPPPPSFTSLPSCPALPSQVSKPLTNRSLYSNSMTSVTIDSVTSMNHRTFSMAQALEEKKKNSLNPRPLKLKPFFWNKIHIPHEKTAWNPSGIDPARIDLKKLYADFAVEPSGSDTKNKEKSDLNNGKITTLLEINRANHIAILLASLKRPNSEIRQAILSLEDDFLSLETLKALRLFSPTPEEIKAMNSFTGDLSQLASSDKYFFSIKDVPRLQSRLCSMIYRRRFENEIEELDPEMKVLQVATSEVHKSKKLKKLLLKSGMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKH7</accession>
    <name>A0A5B0PKH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKH7"/>
    <dbReference type="OrthoDB" id="2506420at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="176" mass="20615" checksum="83C7519567E459D6" modified="2019-11-13" version="1">MDQHKMTTERRALEAKDGGKATQQGIKGFLEYKPAFVNRILNQLVMIWQIRQALPWSRIEDPFLRAAFQYSNSKAQLYGLQWSADESKKLYDMLKSHVFHELNNLNTQFTLIHDVWTTKGNRFAFIGAAVAFINNEWEYTVRHLTLKMIPWKHAGHLLARPIAKLLKKNQLYKKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLK2</accession>
    <name>A0A5B0PLK2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="8">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_035424</name>
        <name evidence="15" type="ORF">PGTUg99_005021</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of the nucleoside 5'-monophosphates deoxyadenosine monophosphate (dAMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP) and deoxythymidine monophosphate (dTMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HDDC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="8"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000070">
        <property type="protein sequence ID" value="KAA1133733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLK2"/>
    <dbReference type="OrthoDB" id="10254258at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000011">
        <property type="entry name" value="HD domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0170">Cobalt</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="13">
        <location>
            <begin position="42"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1102041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1133733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="210" mass="23929" checksum="3B41FF1D7799B78E" modified="2019-11-13" version="1">MAETTPSSNSGGLLELMHIIERLKTTKRAGWVRLGAQAPESISDHMYRMAMLAMLSEKDADLDISKCVMLALVHDFAEAEVGDITPHDGVSREEKHRRESSAIEKFTTELLPPQSIPSQRLKSLWLEYEEGQTREAKFVKDLDRFELALQGVEYEKRDKLASIQSFFETTIPLIKHPEVRGWANELMDERASIQGSEPFVKDYKDVRPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMC7</accession>
    <name>A0A5B0PMC7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PMC7"/>
    <dbReference type="OrthoDB" id="2506685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="714" mass="81680" checksum="5CE1519CB19C130C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT62</accession>
    <name>A0A5B0PT62_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT62"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1103139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="68" mass="7576" checksum="E7244095BD084687" modified="2019-11-13" version="1">MASYLNGLLDTLEGHNTGGVMQISSGQIAQLEKWSMTAPADLRPKYWNWEDPAGPARSFVMHKLCPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXE4</accession>
    <name>A0A5B0PXE4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXE4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="145" mass="16153" checksum="149F77A93CF6DAE5" modified="2019-11-13" version="1">MIKLDGSLTDQCSPGYDRVFIGRLRWLALQGQPSLAGHLMSSSCLPQEQASVVDQACPPIQQRWPAHWGNLSAAVSGMTPHQPDCYANRVYRRCWHDRQIEAFKLLDISKGFQLKQGYNGGLPVNICGAPSFDLEPKKLDFPAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXT7</accession>
    <name>A0A5B0PXT7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_017319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXT7"/>
    <dbReference type="OrthoDB" id="3359487at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="299"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="393" mass="44051" checksum="5A05A1BBB5A6AA34" modified="2019-11-13" version="1">MWRTEPRPSPPHSAGRYHYYRIDNRTDRRTVTIDYRRQTPHHQSIDLQTPYTASSLVIETRQSEESTSASEARHVKGCPTYLAAVSERILSLLARRRGHTIPKKERERARRHQAPSPHLQRVAQSSASSTYTSYREPELSDQRDKHGRRMIAYPCKLCGTRINRPTSDSSCSNLNKHALLCRRKHNESQNMQSLANLGITGTGDINPTEVPQLCAIWCAEAARPFSSWIAEAIRLAREMWVSNYKPRPVTPASSAPTTSSKPITGMLASLGRAAATRGGDSSADAFDTWLSGGLVLNGDELVNPLKWWMEQKRSGNSHRGLVHMALDVLACPATSVDVERAGMTVAFYSKNSKIQEGTLTAWKQGLQDKKKSKQKSKRKVIVLDDDEDENSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZ14</accession>
    <name>A0A5B0PZ14_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Suppressor of glycerol defect</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SGD1_1</name>
        <name evidence="6" type="ORF">PGT21_029714</name>
        <name evidence="7" type="ORF">PGTUg99_015364</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZ14"/>
    <dbReference type="OrthoDB" id="361797at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF4">
        <property type="entry name" value="NUCLEOLAR MIF4G DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="5">
        <location>
            <begin position="639"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="27"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="196"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="307"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1106146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1109198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="928" mass="103215" checksum="C709C6EB778F52E9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZG2</accession>
    <name>A0A5B0PZG2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fungal-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZG2"/>
    <dbReference type="OrthoDB" id="5584477at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040976">
        <property type="entry name" value="Pkinase_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248:SF2">
        <property type="entry name" value="FUNK1 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248">
        <property type="entry name" value="FUNK1 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17667">
        <property type="entry name" value="Pkinase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal-type protein kinase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="245" mass="27553" checksum="90FA3040403C2E5A" modified="2019-11-13" version="1">MFCTFTRSLELSPPKALSLGLLFVEPQCSSGSLSGAIGSESFDVKASKENLKKFFALISLSLTCNKRLLGFDPTFIDDQGPHTAIQIVTDTGTQELVIDHPHIFRAAGICGRGTTCWKAHLSVDERQTFLIKDSWQPKDRREEGVMLRDVTENNVPHVARYHHHEDVHVAGNSVDIESHVRRGANFQTCQTIKITDEPNDPQVPHGFINRVHRRLILKDVGQPIWTVDSPVHLLKALELKDMRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0C8</accession>
    <name>A0A5B0Q0C8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0C8"/>
    <dbReference type="OrthoDB" id="2507825at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="73" mass="7680" checksum="83F0012160DC0ABD" modified="2019-11-13" version="1">MNASNPAANGVGQGELDDNIEDLLADDISVSEAPSEESGADEPTGADCNINTTRDSPASDPRPKKRGRRERTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1B1</accession>
    <name>A0A5B0Q1B1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036648</name>
        <name evidence="2" type="ORF">PGTUg99_008875</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000309">
        <property type="protein sequence ID" value="KAA1106809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1B1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474359" evidence="1">
        <location>
            <begin position="24"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="180" mass="20127" checksum="B595BFB0EEC4BAC0" modified="2019-11-13" version="1">MLLKSLLMAFYVLYQHMFSSSLAKNILKRSMGRLGIDGSSSSGTESYFDGPLYEFKELSLLQGEVRSVSWYCTPTEITFESNGRHRTVPYEEFLMLGDSDGISESDKSTITNALLSQDKGSQAARIKASRNNAISADKKKYIWPNGPPNLTKESQTFNSSQGHSKITYTFFHTPVVAKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q458</accession>
    <name>A0A5B0Q458_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031992</name>
        <name evidence="4" type="ORF">PGTUg99_010456</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q458"/>
    <dbReference type="OrthoDB" id="79687at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14011">
        <property type="entry name" value="PK_SCY1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF6">
        <property type="entry name" value="SCY1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="64"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="676"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="712"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="780"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="746"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="780"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="803"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="827"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="860"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="898"/>
            <end position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1107918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="922" mass="102297" checksum="9056B52241DE8A63" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q488</accession>
    <name>A0A5B0Q488_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-hydroxyisobutyrate dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_031460</name>
        <name evidence="11" type="ORF">PGTUg99_015124</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17681"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIBADH-related family. 3-hydroxyisobutyrate dehydrogenase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.31" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000306">
        <property type="protein sequence ID" value="KAA1107948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q488"/>
    <dbReference type="OrthoDB" id="435038at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000006">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000746">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981:SF7">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="8">
        <location>
            <begin position="50"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="9">
        <location>
            <begin position="234"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1096911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1107948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="373" mass="40126" checksum="C7EF0FDFA38DA105" modified="2019-11-13" version="1">MASINYRTMIRSRGYKIGTLAARRLRIVEQRYGFSTTGVHRMAEHRRENSIGFIGLGAMGQEMASNLLSKFPSPISAFVIHDHLPSSVARFVDRHPSLNADSTTRIVTATTPASVARQASTIVTMLPSSAEVSAVYLDPNTGILRGINEEEHSSSATLCIDSTTLDVRCATTVSEKLRAAGAVEMIDAPVSGGVVGAREGTLTFMCGGPEETFNKAQPFLNAMGKKTIYCGKSGSGLAAKLTNNMLLAVSMIATSEAMLLGQRLGLKPDLLARVINSSSGRCWSSEINNPAPGALLPSKRTPADDDWQAGFASRLMAKDLGLALQAEPALLPEHLPVSNLVKEIYLRLTSDPAFSNKDFSVVYDWLQNNHSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q556</accession>
    <name>A0A5B0Q556_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q556"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="52" mass="5669" checksum="3973582633CE8E5D" modified="2019-11-13" version="1">MCRPLVFVGSSSPSSTTKNRDKSSHSARPVFHTPNPSLSQSLSASGFHRRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5R0</accession>
    <name>A0A5B0Q5R0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017639</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5R0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="196" mass="20899" checksum="916DE900CF41473D" modified="2019-11-13" version="1">MMVIEVDGAPSFSSTSSASTADSALGLKAYVAACSSVQCALPPLHPTIPTVSIGVPLVRVSILLPLGLSPAEPSLSPYPAHTIPPPNQSSTPHPIIDQPVPVTPSPSLPVENYYLLALDMACDYGLQIYGLDRILSLSQSTQDIICLGSHSSSSVLKPKPNILKPPSQTFLIQDLFTQSSAINPFNHLNQACWWDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6D2</accession>
    <name>A0A5B0Q6D2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6D2"/>
    <dbReference type="OrthoDB" id="10284998at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="119" mass="12987" checksum="B13357DD9060C5FA" modified="2019-11-13" version="1">MAIITAARDCSDLSVLPPPSRKHLAALRLLSARDASHHPATTFFQLGINTDSFRSRSSLQLATAVNSVFFIYPGFFQSQASHNPVTASNLEYFINTCPFQSQLSLQFATGFVSFPGLTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7B1</accession>
    <name>A0A5B0Q7B1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q7B1"/>
    <dbReference type="OrthoDB" id="2506242at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="314" mass="35001" checksum="8B158A4B5D9DE783" modified="2019-11-13" version="1">MPTAAKDPPWPDTVGKTLTPSFVFALVGSKMVPRSLPGSLLGHWRWRFQDKRQANRPFFPSSSSSTPIPSSMARSAFNRPPTPYPAQPHPQPFTRIEMVDTVPMNLDAAVGPPTSAPPVISTLPLAARIAPAGAIPPPFGRGPPPAYFGPNPVRPRPSHGYRRYNINGSRGGFRGERLLTLPPRNLNSPIAESVHSSRAASPQASESHAGTVEDFLIPQSRVPSPNPPGFYDPEDYHRLTRDQVESLLDRRAQPERFNRYDFTPQHFTDLYESITTAMVHHFTTYPAANAAERDSRVRLFRFATCRYRTMTRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q8T6</accession>
    <name>A0A5B0Q8T6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 74</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_024124</name>
        <name evidence="11" type="ORF">PGT21_032878</name>
        <name evidence="10" type="ORF">PGTUg99_030838</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in vacuolar protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q8T6"/>
    <dbReference type="OrthoDB" id="2189106at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3630.10:FF:000002">
        <property type="entry name" value="Vacuolar sorting-associated 74 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704:SF2">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3 HOMOLOG SAURON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704">
        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1084312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1109670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1119738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="394" mass="43361" checksum="2D6423ECA553C8EB" modified="2019-11-13" version="1">MATSSNAGLSRRRVAGSNNNGSPSAMNGPLSPDGSSAYNKTFQNDQSSTTNGANSSNNHTSNRDQSNNDPSQNHKIAYDPRDLDDSTESSLNPKLTIMEEILLLGLKDKQGYLSFWNDNISYTLRGCIILELALRHRIAMVRDPNRRRFPLPDRYIEVIDDRLTGEVLLDEALKMMKASEKMSVGTWIDLMSGETWNVMKIGYQLKQVRERLAKGLVDKGVLRTEKRNFLLFDMATHPISDSTFKDDVLRRTLSILTSRTVAVPVSDLYQANVRYRTLRAVCMVCASFAANVLENALVHLSYDAREAAFQKCDELLAEFSQWPLGAAGAGSSNPRSPASAPPSVAVGSGGSDVTNANLLEITRVSRAEMVPGDELQIEVVAAVLHVFSKMDSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBG9</accession>
    <name>A0A5B0QBG9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_024850</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBG9"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1110547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="163" mass="18419" checksum="84862DBF6AC2B7CE" modified="2019-11-13" version="1">MYLVNRKETLPAGEVNKRTYDQFSSQSYWTNDFGNFPQQDAANFHIIPHEPLSVYLRCDVSSKQHDASGDVPIVKLKRFPCAECGQRFAQAFNLQTHIATHAGMRPFSCPADGCSKVFSRRHDLGRHVGAVHREWLASKNISVEQAVKPLRWKNGTSNKSSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDE7</accession>
    <name>A0A5B0QDE7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Leucine-zipper-like transcriptional regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LZTR1_4</name>
        <name evidence="2" type="ORF">PGT21_000777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000022">
        <property type="protein sequence ID" value="KAA1111248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QDE7"/>
    <dbReference type="OrthoDB" id="2496273at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="274" mass="31346" checksum="CE430698084C6CEA" modified="2019-11-13" version="1">MAANEKKGWEQFVLHLLSRIDYVAQVNTNGPQQDGTMWADGWRKCSKNTEHFGRFCCVESLRKQMKLLNFDPDDQKARLLQAGEWISSHLKDFAPVVHDNYHQLLTINQYPSMNHTEYGQPYTTSDFASFLTFTMFDFHNLPHNDNDVNDWTLVGWIPIFNPKKSDNPQILADQDFDMIGGQFSFRDFQICLDLTKTLGVTLCVFRSKDFRHQTLPGASPSGKYTRIGFSCQINQSMASAVTAYLQGNYEKKLNIGGLQNQVDNANAPKKKKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QE88</accession>
    <name>A0A5B0QE88_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_001816</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QE88"/>
    <dbReference type="OrthoDB" id="423559at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF14">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="154"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="567"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="48"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="253"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="479"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="278"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1111497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="737" mass="81885" checksum="7F27B226A1731E95" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHN5</accession>
    <name>A0A5B0QHN5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHN5"/>
    <dbReference type="OrthoDB" id="2496740at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10741" checksum="2D2AD2589831DDE6" modified="2019-11-13" version="1">MVAPHTPPVTSPFAHPGQRPAKPVRFGSRNCRPKCCLLVRVRTARQMPMVTTSATHSYRFTDDGGMELFSEEGKLDVLQRRVYPEDMMPSPERPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QID6</accession>
    <name>A0A5B0QID6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein disulfide-isomerase</fullName>
            <ecNumber evidence="3">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_017257</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QID6"/>
    <dbReference type="OrthoDB" id="10264505at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00238">
        <property type="entry name" value="ERp29c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1150.12">
        <property type="entry name" value="Endoplasmic reticulum resident protein 29, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011679">
        <property type="entry name" value="ERp29_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036356">
        <property type="entry name" value="ERp29_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF11">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07749">
        <property type="entry name" value="ERp29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47933">
        <property type="entry name" value="ERP29 C domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023145940" description="protein disulfide-isomerase" evidence="7">
        <location>
            <begin position="26"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="25"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1112968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="266" mass="29181" checksum="ED21B06A7727836A" modified="2019-11-13" version="1">MNRFNFRTAIGLLVSSSLLSNGVLAGLEDAKHSTKLDHTNFDAQIQVPEVGTLVAFFAPWCGHCKSLLNPWDQASKAFETDSKCRIAHFDADASANRDIASRFSVSGFPTIKFLFKDKSKAAIDYQEARSAEAIIKFLNKQCGTFRASGGLLLPEAGRVAGLDEIAKSFLGLSSEERPSIIEKATELASSASEKMASYYVKVMNKLATDESWLSKESERLKKLAEKGATMASEKFEELQIKQNILQAFMTVKEAGEHVVDKVTGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QKB4</accession>
    <name>A0A5B0QKB4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QKB4"/>
    <dbReference type="OrthoDB" id="2507159at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="137" mass="15321" checksum="2DE0296DE40AC3C6" modified="2019-11-13" version="1">MVKWPWTKTEKKLARMKFRLELLPGARSKREWLMEPSRGLTSAPEATLHNDLDKKLIELVYDPTIPDDPSSKPTARGRRRQPPSGSRKGRRSGPLSGSSTIGSPKSQQTMSEVSNSPASQRTTLEESDIDHESDVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QM88</accession>
    <name>A0A5B0QM88_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QM88"/>
    <dbReference type="OrthoDB" id="3205926at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="113" mass="11717" checksum="8D11CCA8DD673CA8" modified="2019-11-13" version="1">MMIQTNGISKLFNVGRSGFVLRSTGNQLLSTSANPTRGPSRPSKETSEQAVGGTTDDIAHSKGAFDSDEANPEKSASKISAGGISMEQSAANEASSLSSQKTSVKKKAPNTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQB4</accession>
    <name>A0A5B0QQB4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQB4"/>
    <dbReference type="OrthoDB" id="2508625at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="192" mass="21259" checksum="115088EDD5AB0B71" modified="2019-11-13" version="1">MPAGGQPLYCTIAFNSSVGLHVVLATLKAKLYNTHSRPNSHPLRFPHHSRARRLTKFPHSSMQFLIHIIALNLLVCGFCIANEQQDKDNKCTLFCYRPEPESPLQAICGTGNRNRFEAEPHSYTVKPANPTKNLPGGIIGYYNCVGTQTDNSYCCNTPINFPKSNVTTIPHKQFIAQCTATGPMKVTPKQCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWX5</accession>
    <name>A0A5B0QWX5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023931</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWX5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022860001" description="Secreted protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="9201" checksum="AA9AE51C7E8EB241" modified="2019-11-13" version="1">MAILPLGLTFWWLAHPVHVLLLLTTTSDELIRAIPTPSRPSNQRSHQGSFRSIRAESCTGLPRIVGHSPNPCPESGPNPTSVAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXN2</accession>
    <name>A0A5B0QXN2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030633</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXN2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1118061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="201" mass="21713" checksum="F22629273FE814F2" modified="2019-11-13" version="1">MVIMASPLRLARLCGGRPLSKVDLNPALFIRSEQRKPLVPLCGVKPPGIVMEFITPRRIYARLALPNGPKGGFLGTDLKCSIPSPGRWRVSSDGHIESSPPVRGAESRVLARPKVALQRRRKGPYLSAPRGCSRAWQFNSRRVFVQPAVLGHSPDKIQIHCTNTPDGQLAALCVSLSGPTTSTATRIIPLCPEVGQEGKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2S4</accession>
    <name>A0A5B0R2S4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035346</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2S4"/>
    <dbReference type="OrthoDB" id="10276935at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="376" mass="43133" checksum="99A6B8B0A87F9BEE" modified="2019-11-13" version="1">MDELDTLASHQLRHQAGFAVKVLERLANCDYDLGLPDTPSDLSIDKLRSKEYLLIELHSALLPLLRQHITSLSPALRELNQAQGKPTPTLKLVIEILLKLELTLDQTIRTLNDLIPGKLPKPSQTNDQHFKEFKCFRLRGFERFIKRVMQAQLATFFSKSRQLIETFTLPDQSRTPVTTSSTKAILSIDFTIVWLKGSELYHIYTNTWVFSLEKIDTTWDTLLAVADPSHPRHIELSRSFKPMVKLSKLFFKKIATEGMTNNMAPIFTEMSSFQLDLLGTTAEKITESLVALVSSLEHDDETQPNFTTTLIDHVKNLISQFQTCVLLTDLYIFPLLTKIKDVLSQIYYKNWIVTWNTLFYQATHNAIQACEAFQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2X4</accession>
    <name>A0A5B0R2X4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2X4"/>
    <dbReference type="OrthoDB" id="2498281at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="507" mass="56194" checksum="43123FC2999B1451" modified="2019-11-13" version="1">MEPADYSDYLDGLQSLDQVSNWLDDPLPGGDSLSSERRSASVANTSSNTQQQVINPKDEANNDNDGEDFKVSIEYKLFVTLKNNKSKPTNTRGGNKRKAVTADTPDKYARLISKPNKLSLTWNSAHTSLAVFKEAVLEVLRALEPDQVFGHAEKQEREKNLCWYAIIPYGGQFIEKNQTLLDNSKIFTRFVEVAEAKGEAKIYLVQNDPKTIAERNEALKQLSEQDKENSDNQSAQAEPTAGASFNRQVIENMWLLRATHMPSEQLTGSMELPVMIDPKDSKRFIALSPDRISLWARAMTKAEFKGITAGAKEEGDNAINVRARTTEDLASVPRTPGSNRSPAGTASASGSTNHINLSPIQMGMGAYPPAMSWPSVNPWGWGPPNPNFYVPYGGMQHEPPPGWQPNPFLAHNSHLAAGTMNTGPVSSPPASDNTVEINDYLSFCHIDHNCESVQKALVDYGITHYQEFENIKPEELEVFGVKKSKARLLVSNTKKYGRTLTKRRLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R348</accession>
    <name>A0A5B0R348_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R348"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="11778" checksum="E97189C804D1AF1A" modified="2019-11-13" version="1">MDALSSSSGSSRRAGFYTSSPEGNPSNKHVHQLVGRKPFQQAGMYTGSSGGVPPGELVSIPARRKKTLPTSMYTSLSEGKPSDKHVHRLVGRGSSRQAGDSPECFAYISLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R562</accession>
    <name>A0A5B0R562_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_037170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R562"/>
    <dbReference type="OrthoDB" id="10324058at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1120115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="14156" checksum="C0F9479B1974A274" modified="2019-11-13" version="1">MYPPIVSSTSSPPPAVPCRTQLVVSNVIIPSALSATEDQVGAAYERAKVKIINVCNRLESSHVPLPVLVQDPFPSKKDRTPPVSNVGKQGYDSFVTSLKQNICKGDITQAVPSQRLKKETRLHLFNVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RAM5</accession>
    <name>A0A5B0RAM5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022067</name>
        <name evidence="4" type="ORF">PGTUg99_007322</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000216">
        <property type="protein sequence ID" value="KAA1122796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RAM5"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16982">
        <property type="entry name" value="CID_Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000016">
        <property type="entry name" value="mRNA cleavage factor complex component Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054127">
        <property type="entry name" value="Pcf11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047415">
        <property type="entry name" value="Pcf11_CID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21936">
        <property type="entry name" value="Pcf11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="51"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="652"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="760"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="685"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1080807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1122796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="801" mass="89598" checksum="24095B9E0DD1D149" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RB86</accession>
    <name>A0A5B0RB86_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_007252</name>
        <name evidence="11" type="ORF">PGTUg99_003609</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000213">
        <property type="protein sequence ID" value="KAA1122937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RB86"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="9">
        <location>
            <begin position="291"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1085440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1122937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="426" mass="48100" checksum="EA14EB6EC0C85F82" modified="2019-11-13" version="1">MIQDIRLIPTIPFAGTHGLLHALQVLLQILFGLFLGAMTIPSQSWPAHVFRLGLLVWLAPKILDYSTSRSYSFGNMLADQLMLSCFGWNLLTRLLELSLLPLISPAPPRWIRPTKEELARVLKLRQQPASNQHPHKLDQASEELVPTSWEPVPFPAPFSLDRVLYAYDYLMLIRPSTSDLLPWQFRAFDWSLPAMSAGGVAGRRFGKPETGPRLALVYLFVYLVCFVYVDNVAIRHIGQITVAELGLAHQLLLTISAGCLISLSAGPSEAFIFPLLLSRRILPPTALLASFNQPYLASSIQDFWGNRWHHRVRRQLTRLGSLFPLGGTRTGNAFWAYVLSAMLHSFIICRSKPEPSLSDPTSYVALFFDRPTVIFFVSQGLAMIVERHLVPPGLRRLWLWLTIIFAGRWYIDGILNSYLPPLPQTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SA45</accession>
    <name>A0A5B0SA45_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003053</name>
        <name evidence="3" type="ORF">PGTUg99_035200</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SA45"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="794" mass="88927" checksum="41594A31BED84385" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SFV7</accession>
    <name>A0A5B0SFV7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin reductase like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_032340</name>
        <name evidence="6" type="ORF">PGTUg99_004305</name>
        <name evidence="8" type="ORF">PGTUg99_022441</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the flavoredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000424">
        <property type="protein sequence ID" value="KAA1084930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000039">
        <property type="protein sequence ID" value="KAA1135404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SFV7"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798:SF5">
        <property type="entry name" value="FLAVIN REDUCTASE LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798">
        <property type="entry name" value="FLAVOPROTEIN OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="5">
        <location>
            <begin position="118"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1084930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1094897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1135404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="324" mass="36041" checksum="58F98855A39AA17C" modified="2019-11-13" version="1">MQIGLFKRLTPWPRVRKSSHQPTLGILIPGSHLTKHSVVITRMSHHPPFKQVEASRPDFDSGHLSRYTKTPQPDWQVGQGANNAPLPGAKIYQSPAPTKTFVPYESISPSEMYKLMISSVVPRPIGFCSTLSADGNTNLAPFSYFNAAGSDPPCLMMSFTATSVGGDKDTIANIRTTKEFVIAIISEPFIEAANYTAIDAPSEVSEWLLSGLTPEPSIKVKPARVQEAAVNMECELEYLHEMFDPSNPKKLTQVIVIGRIKAWHIKKDVLVDDTPLVALEKLMPIGRLGGITYSRTIERFELPRPIWKEEKEKDEVKKVLSQPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LM00</accession>
    <name>A0A5B0LM00_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LM00"/>
    <dbReference type="OMA" id="NLPIRPQ"/>
    <dbReference type="OrthoDB" id="10269404at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022889168" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14256" checksum="DE9B11630C844172" modified="2019-11-13" version="1">MQFALAIAVCLSFIYSSIASPVIIDKGKYRIDTNQPYHATGFRPPNQGPYPNVDWNQGYPYPSNFGFPSSYSYADFDAWYRQQQYMNSGPGYINGGSSTDQGYNFDSGSGTYFAKEAKPEHKGKADAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNI7</accession>
    <name>A0A5B0LNI7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone deacetylase hda1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">HDA1_3</name>
        <name evidence="3" type="ORF">PGT21_009545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNI7"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="2">
        <location>
            <begin position="60"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="119" mass="13623" checksum="CC0414DA0CC6DFFA" modified="2019-11-13" version="1">MAHEKQNGTSTSNPHHAPPSTSLSRPSRTGICHSQQMLKHWKPDVQFDTIKDQDDENEEPERPSRIARIIAHVSKQKLTDNLEHIKIRKATKEEIMLVHSEGHWKRIEETASKSCANIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPX2</accession>
    <name>A0A5B0LPX2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014045</name>
        <name evidence="2" type="ORF">PGTUg99_012556</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000314">
        <property type="protein sequence ID" value="KAA1104969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPX2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="76" mass="8183" checksum="6BCD3F277A477ADF" modified="2019-11-13" version="1">MKTIGRSGEPLKEFSGAGYSSFGVRGNTVLLHTCQLAASGHQASTFTSSSDLIENQHFEIARRGSCKPALLLKDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LS67</accession>
    <name>A0A5B0LS67_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012027</name>
        <name evidence="2" type="ORF">PGTUg99_025859</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LS67"/>
    <dbReference type="OrthoDB" id="27237at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010770">
        <property type="entry name" value="Ecd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060:SF0">
        <property type="entry name" value="PROTEIN ECDYSONELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060">
        <property type="entry name" value="SGT1 PROTEIN HSGT1 SUPPRESSOR OF GCR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07093">
        <property type="entry name" value="SGT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="679"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="617"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="694"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="735"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="745"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="787"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="797"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="819"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="905" mass="100854" checksum="423D2AB1832A44E9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LTT8</accession>
    <name>A0A5B0LTT8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LTT8"/>
    <dbReference type="OrthoDB" id="10670227at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022874251" evidence="1">
        <location>
            <begin position="23"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="618" mass="72214" checksum="5843484306CB5F58" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXB1</accession>
    <name>A0A5B0LXB1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_026496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXB1"/>
    <dbReference type="OrthoDB" id="2506069at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1069497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="380" mass="43245" checksum="24F273B7DF1FD58B" modified="2019-11-13" version="1">MRLARRWDGIQPQVLDLRMIPTAALSAFHGFETLTKKYIQTFVREIKTEVMIVQQPFREVTVMEAKEPSEFERFFQIEKVLRESNPVNIDTLELELVLVGRMLDGYVPTCSHPLTARPLLNLSRLKNVKKFHIASPISLSLPQNLLVQTVVNMKVLTSFSADNIGYEETVPHPETLGGIVTRQEGETGDQSQEPPTSTLVGFLSNLKCLKSLKMYDSLCVDHTWSEMAWQSPLAEIVLLRCPNLSSLSLQRLSKNFNLSLRAMEVSTIGISEIEQIQTNSPGYLWEILQSQNTITFKKLRSLKLVGVNMGEHLLKFFHDSPKLRRVVIDSPNIRAQTLFKSLEKWPSLRDLDCRIRGGTGSLDLIMYCLTQGVFLDESFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LY86</accession>
    <name>A0A5B0LY86_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2-dehydropantoate 2-reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_007985</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LY86"/>
    <dbReference type="OMA" id="HIEVECI"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000609">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF26">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="4">
        <location>
            <begin position="7"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="5">
        <location>
            <begin position="222"/>
            <end position="350"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1068989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="40677" checksum="10C0CFAF4F28E36A" modified="2019-11-13" version="1">MVSEIQVLIVGAGAVGGFYGSRLHRPEEGVKVSFVCRSNYEEVNAEGIEIESRTFGKHRIRPERVFKSIEHAAELGSSGTGGRWDYVVLCTKVLPDRVDDPALLSPLLKDPEDGRHPPTLVLIQNGIGFEDKHRQRHPKVPILSAVTVVNAEQLQPGLVRHNHWTRISIGPYLDFSAYLEHPHPSVDPQLELHSQSQLELFVKLLRNGQINDAEVYGERDLQILRWHKLAINASMNPTSILSGGMPSSEMVKDGELREHLSGCMEEVFEAAKKIFGIASFPAEFASIERILKSTERAGERATIKPSMLADWEQGRPLEIQAILGLPIRIAANAAIKLPRIQSMYAFLTQLELARSQKKEANQHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYW3</accession>
    <name>A0A5B0LYW3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SCF ubiquitin ligase complex subunit cdc4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CDC4_2</name>
        <name evidence="6" type="ORF">PGT21_029072</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="protein sequence ID" value="KAA1069601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="7"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
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        </location>
    </feature>
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        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="675"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="717"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="85"/>
            <end position="196"/>
        </location>
    </feature>
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        <location>
            <begin position="391"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="835"/>
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        </location>
    </feature>
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        <location>
            <begin position="85"/>
            <end position="123"/>
        </location>
    </feature>
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        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="835"/>
            <end position="858"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1069601.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="944" mass="104787" checksum="344D4361D76D410F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2W2</accession>
    <name>A0A5B0M2W2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017805</name>
        <name evidence="3" type="ORF">PGTUg99_013028</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1070609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2507854at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="70"/>
        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="11"/>
            <end position="22"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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        <location>
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    </feature>
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        <location>
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            <end position="989"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089795.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1087" mass="119755" checksum="12E621F70D00E658" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M699</accession>
    <name>A0A5B0M699_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_050318</name>
        <name evidence="11" type="ORF">PGTUg99_050108</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M699"/>
    <dbReference type="OrthoDB" id="2506934at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025525">
        <property type="entry name" value="hAT-like_transposase_RNase-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14372">
        <property type="entry name" value="hAT-like_RNase-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="hAT-like transposase RNase-H fold" evidence="9">
        <location>
            <begin position="469"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="8">
        <location>
            <begin position="610"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1071769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1126847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="712" mass="80470" checksum="8F92F7079A02A3ED" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7V3</accession>
    <name>A0A5B0M7V3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032071</name>
        <name evidence="3" type="ORF">PGTUg99_013172</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7V3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137174" evidence="1">
        <location>
            <begin position="23"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="85" mass="9295" checksum="64B6EE32E32C6640" modified="2019-11-13" version="1">MLISAIHIQFLLVGLLTVSVTPSSIWEIGHPIEADGCETSGGPLHGLENGESHVPQQHGEYTNSVFELPRKLQVPKLELIHSNMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAQ7</accession>
    <name>A0A5B0MAQ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">H(+)-transporting V1 sector ATPase subunit D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">VMA8_3</name>
        <name evidence="4" type="ORF">PGT21_006461</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAQ7"/>
    <dbReference type="OrthoDB" id="7676488at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1073253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="193" mass="22144" checksum="F99E1782936F6214" modified="2019-11-13" version="1">MMSLRFNPLALHEHRCRSPDGDIRYSRRSRRTHQTIRAITQKIDHTKREMGRVMQLAAFSLAEVTYTSGGDIGYQLQESVKEATFKVSTHQENVSGVNPAHLWVDRTKAGGSAITKAREIYAKATETLVELPPFRYYQSELDEGDREDFFRLKKVQAKKKERTAIAEAEKRERNAVWPGRLSSTMRRGGQIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCL1</accession>
    <name>A0A5B0MCL1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCL1"/>
    <dbReference type="OrthoDB" id="10298115at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="422" mass="47244" checksum="26F8639E12BB92C7" modified="2019-11-13" version="1">MVETSHLNNSSKSSNAENVSNNQRTEQETDLLDYSEAMTRDTPSAPLQPSGFEGLGSSLPSVTDTATRPQAAETSRSEQGISPALINSRATSLALETASNRSTPVPRPVDRLVFPVNSTTVTGRSILAPTPLLPNPNRFSALSPSPAPLGLDDIIVDNQELETVIHLFNEQWNTFVQARENQNTRLMRMALVQAISSQEEIRLLAGGAEMLRICKNWIAREELADLERSEAATRSRSTQRLAITQTAHQHTISPSPAPQRSIRQSSDMTYLGTGQVQQQSNPPPPPPPPSTPHPSEVARQTQHYQQQPLNHYQQRGYHNPQPQQERFVQNYAPPQHQEVQVAQNHGFQQPPRPPRQGNWRGSGRNRRRPQDQTTRLLEVGEYLMRAERIAGRVFRDHARGRGRGSRRRGPPRQEEPPAEHRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MD38</accession>
    <name>A0A5B0MD38_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MD38"/>
    <dbReference type="OrthoDB" id="5577218at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013635">
        <property type="entry name" value="Ice2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31726">
        <property type="entry name" value="PROTEIN ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31726:SF2">
        <property type="entry name" value="PROTEIN ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08426">
        <property type="entry name" value="ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="397" mass="43964" checksum="78DAFFCFBC08621F" modified="2019-11-13" version="1">MINRLITRAGRTASFLQILLYLPLALDIAGKDCFLALSASLALYYSALSTLYLIFRNTRFAFVSKVFGLLQNLVIPTFLLFWLNIYSPDESKNIGNPILASNLIGRMLSLWEVVLTWSTPVFVLLEGISTLLCIQSVGQISRYLVEAKNESYSFVFLVLSAAVYVGSAYFLFDSYAYAARESLSATLIGVSVTSVAFLSGIAISNRKGNVVETSLMMAYLSYNIYWLSSEKLDPVSFFSSFKSEAVPPLPPMILRSATAIIGFVSTTFGAGLDFLLACSSALPLPVFINLLYRVVALYGASRIVVAIKRANSGFAYTRRLSDEEPMVRIMTVIVSYSRFVLISVYTHLLLLNNEGHQVFWRWINVFVTLGLWGVELMIGKEEGDNDSIVTGLGLKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDQ1</accession>
    <name>A0A5B0MDQ1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_021154</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDQ1"/>
    <dbReference type="OrthoDB" id="2502266at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="842"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="63"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="225"/>
            <end position="291"/>
        </location>
    </feature>
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            <end position="430"/>
        </location>
    </feature>
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        <location>
            <begin position="470"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="865"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
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        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
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            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
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            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="268"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
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            <begin position="366"/>
            <end position="376"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
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        <location>
            <begin position="868"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="880"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1074802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="911" mass="101611" checksum="7C7B6C857EDA4571" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGQ3</accession>
    <name>A0A5B0MGQ3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_029982</name>
        <name evidence="3" type="ORF">PGTUg99_028425</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGQ3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366134" evidence="2">
        <location>
            <begin position="21"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1091276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="440" mass="49561" checksum="B82B44B80F42EF3E" modified="2019-11-13" version="1">MLLNQILLAFYLLQYYMASAHPHSFTKNPIRSGIPSVVPGQQSHRLKKRKAPLLMENSFIHSQQAGIELGRSAQTKEQGSVYFTDESSKFMNEEHGVENPTEKIDGFNGQSGPGRHNIYQTMNTVPEKGPFSERKRHISSEGQLFTWKWFSTDPMIIIEIEGEEYAIPVEEFQKCKRDEMTFTDQEKQEIKRKVLKEYGALESVELKLILQPNPYRQLEKKILFLGISNTGKIANLRKMPQGGGKIYETENYLATGHDKELVTFKCWATKAELVDSSILSPDESGHSPRFGKDLVVYTQDEIEKTFSAEKKPNVSKNNVENQNDGSNNVQKEGNPKIKQNHADEVEVAQDSQEDDIKKSGGVGFKNHGGGKNDVPKGSHNPGNHNGGPTLQAFASSDPSKKTFFEMIKSFFSILKKGFKAMMAKIPFWRKKTLNAPVDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGZ6</accession>
    <name>A0A5B0MGZ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010155</name>
        <name evidence="2" type="ORF">PGTUg99_012200</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000348">
        <property type="protein sequence ID" value="KAA1098653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGZ6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="98" mass="11021" checksum="7DCD3E19FC05DB78" modified="2019-11-13" version="1">MEPPRPNSALEQKPELISQADSIIRGLQELRKFEFSWSDSDPAFTATSLHQLRLEKDGLGWLVGGFYPNSNDNAPAYQERCATHPTYEKTQPQPSNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHT8</accession>
    <name>A0A5B0MHT8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000152">
        <property type="protein sequence ID" value="KAA1075719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHT8"/>
    <dbReference type="OrthoDB" id="2510371at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="14523" checksum="03822A4B36625A0C" modified="2019-11-13" version="1">MPKSSQDNTFKNQTPQAEMTLKNAGVPLETEPEAPAPDIQPATTTQPTPLEEVEYIYDVVSAMKHALIQDHNSELFLETPNFNKVHEEYIKALEVFLERVSAIIKETLEAGNHFIYDSLDIPQISSPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ML65</accession>
    <name>A0A5B0ML65_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ML65"/>
    <dbReference type="OrthoDB" id="2503698at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="36103" checksum="7E47266ABD6A9FA6" modified="2019-11-13" version="1">MSYRASFAAPPNLSHLRFFDWEKAYRQIPTAMDQWPFLLVKNFEDMLLLDTRITFGGVSGCGSFGLPADAWKAIMLHEFDLISIFRWVDNNLFVKNIDSQTEMEDIVSRSNELGVKTNDEKFSPFATEQKHIGFVWNGVDKTVRLPDGKLFDRVNQLKTFAKLERFSYHNVEVMTGRLNHNDLEQWMHTLLTFKPTCLIPNPTPTEIGHTGGIAWLETVAIRLGLLMLESLGIKHGKKFIVWTDNTTTENAIRKRKSRDPSVNKEWKIIQDLLVKLQADIDPRRVRSESNTADSLSRGIGDGHEWRHVVPIHMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNK1</accession>
    <name>A0A5B0MNK1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024207</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNK1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="8276" checksum="8F993A4234820649" modified="2019-11-13" version="1">MPYSALSCRNVPREMQEKCCYKGHYTSAGKYVGLEIRRQPTGSMSRMPTMRSPKTPVHQALLFLFHLNSKPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNS2</accession>
    <name>A0A5B0MNS2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNS2"/>
    <dbReference type="OrthoDB" id="10297080at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="199" mass="22443" checksum="63DDA5B2C8A4FCD5" modified="2019-11-13" version="1">MASIHASIPLHMTRQVSFTDTSYTVLYSPPPIPSNHSQYSQPLYKIVLSKGTSPPFATTGWLTFLGTLNLDSIRETSLCLEIHVDCHSWDILPDPVHRHTLSPIVTGTLCISKLSPRIQGFSLQKGPDFGPRFVLHASPLVNKEHLMGLMPSNVILYSGHLAFEKGRFLHIAAETIELFHNYENPHTYNLRSRSFHWSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRL0</accession>
    <name>A0A5B0MRL0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020430</name>
        <name evidence="1" type="ORF">PGTUg99_007946</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000449">
        <property type="protein sequence ID" value="KAA1078580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRL0"/>
    <dbReference type="OrthoDB" id="10688598at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="120" mass="14407" checksum="8A79DACA9BA9D96C" modified="2019-11-13" version="1">MILTVIPRPAEGHERGDYSYTQELREASRLFLGLENPFQDPLNDPDELWTEKRAKLSVQIERSKAQRPQVLWIFLDLWVRERYRELWTSQLKKTRRSISSHAKGFFNYIFCFSIVRGYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSX2</accession>
    <name>A0A5B0MSX2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSX2"/>
    <dbReference type="OrthoDB" id="10546751at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="270" mass="30186" checksum="FDF6DB59E7AA33F4" modified="2019-11-13" version="1">MSEKSRMLSQKVCKDGSHHLQSRTNFAYPVSFRIIFLSWFTMLMLEIAPAEAMQTALGAGRTVRFSSVGKGMSLEALENGSGLPTKLMDGGQVNQARGDQNMLSKTHIETPGLPPKLENPTALLKATEPVNGGQHVTVKTVPIAKDHLAQLVTHYGDRVQTKDFVKNDKYNVYRALAHFKYRDQEKYGLVAKEIKKYIRNNPSQFKNYRIGIFSMISRKYAADESIRIGFRWYRAVGKEGNAGVIFSSFTKSQPESSSLTVISKTPGFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MX63</accession>
    <name>A0A5B0MX63_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030491</name>
        <name evidence="2" type="ORF">PGTUg99_031206</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MX63"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1081152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1131313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="58" mass="6385" checksum="33A255EC78A55EBD" modified="2019-11-13" version="1">MEESSGTLTDASADSYFRAQTADQEISEPVMKFLYNICCRIDPLSFPPATAKGIQPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZK4</accession>
    <name>A0A5B0MZK4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZK4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="74" mass="8866" checksum="C6306373DEF63C06" modified="2019-11-13" version="1">MISENEMEFVLLDEQFSKHEYVSLLRVAYHPVIMLIDCQFRNLDRYLAGRRRSETHNPYKDLPGYRADTKASRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N249</accession>
    <name>A0A5B0N249_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009069</name>
        <name evidence="3" type="ORF">PGTUg99_015096</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N249"/>
    <dbReference type="OrthoDB" id="2506528at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="614" mass="69609" checksum="410D97E629D0B47D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N310</accession>
    <name>A0A5B0N310_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004260</name>
        <name evidence="3" type="ORF">PGTUg99_029231</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N310"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="146" mass="15491" checksum="3CCD02D103419B54" modified="2019-11-13" version="1">MQHSHGASQDGAHALAESNQPTNASWEGQQSSSAPPNSSANSTPVTMELEATEKVSKKIEHMTAQLDRILTNIKDVDTQLSRLAKSIEFGFGMGGNTAPLEGIAAPLEGITTRLEDININEETLGINSQSANQVGQHANQVDQGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5K2</accession>
    <name>A0A5B0N5K2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_012562</name>
        <name evidence="14" type="ORF">PGTUg99_014066</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="14 17">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1066922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5K2"/>
    <dbReference type="OrthoDB" id="1931232at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04323">
        <property type="entry name" value="AsnRS_cyto_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1910.20">
        <property type="entry name" value="asparaginyl-tRNA synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048952">
        <property type="entry name" value="AsnRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20917">
        <property type="entry name" value="AsnRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="269"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1066922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1083956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="575" mass="64398" checksum="D4205B2EA70D5BB7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5R5</accession>
    <name>A0A5B0N5R5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lactoylglutathione lyase</fullName>
            <ecNumber evidence="3">4.4.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Aldoketomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ketone-aldehyde mutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Methylglyoxalase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">S-D-lactoylglutathione methylglyoxal lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GLO1_2</name>
        <name evidence="14" type="ORF">PGT21_014499</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glyoxalase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.5" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5R5"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00675"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07233">
        <property type="entry name" value="GlxI_Zn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004361">
        <property type="entry name" value="Glyoxalase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00068">
        <property type="entry name" value="glyox_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374:SF30">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE GLYOXALASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="VOC" evidence="13">
        <location>
            <begin position="35"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1084016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="181" mass="20821" checksum="A28679CAFE2A23C1" modified="2019-11-13" version="1">MRTLLERVINLKTHLNLSANSSIRRSHIASQQTFRLNHTMFRIKDPKISLEFYQDILGMKLLHKMEVESAKFTNYFLGFPGSNQDSKSSSPLHREGVVELCHNWGTESDPDFSGYHNGNKSPQGFGHLAITCDDVEKTCAYLEEKQVKFQKRLKDGSMKEIAFIQDPDGYWIEILGNTFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7W8</accession>
    <name>A0A5B0N7W8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAM1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Outer kinetochore protein DAM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">DAM1_1</name>
        <name evidence="16" type="ORF">PGT21_035730</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7W8"/>
    <dbReference type="OrthoDB" id="5586015at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990537">
        <property type="term" value="C:mitotic spindle polar microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013962">
        <property type="entry name" value="DASH_Dam1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28113">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28113:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08653">
        <property type="entry name" value="DASH_Dam1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="133"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1084816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="311" mass="34804" checksum="909812967481720C" modified="2019-11-13" version="1">MNEQQRAKPPKTPLKRVSRNSLRTHTLSGSYPPSSEPRQSAQPSSQQESTPAADPGAHLEHLAPLFVDLSDAITDLHINLSNLDLVCQDLDGFNEAFSAFIYGLRVNSYVTDFDEAPKLKSFLDAQARKEAEREERLREHAEKEARRQLEESKSQRNEQEESFMTNEHSMTTRSANQASTSSNPNQSRSANQRGNNNGSSSTSSSSKIAGPKPKNFTQASKTQQKQLLKFTEKIMVTLPLKFREQQPHRQEMELVISILKLNPNGLLIKDIVKLEPTLAMHRARECATALVAAKKAVKLNKDGLLYKLLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N8B6</accession>
    <name>A0A5B0N8B6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_011098</name>
        <name evidence="10" type="ORF">PGTUg99_013333</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in the regulation of membrane traffic through the trans-Golgi network.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1135966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N8B6"/>
    <dbReference type="OrthoDB" id="2018246at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14235">
        <property type="entry name" value="GAT_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16998">
        <property type="entry name" value="VHS_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.160:FF:000005">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.2, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000008">
        <property type="entry name" value="VHS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052653">
        <property type="entry name" value="ARF-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="7">
        <location>
            <begin position="25"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="8">
        <location>
            <begin position="188"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="378"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="433"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="460"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="472"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1085557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1135966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="58415" checksum="A764E69F9CAC8338" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NC21</accession>
    <name>A0A5B0NC21_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cytochrome P450-dit2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">DIT2_1</name>
        <name evidence="5" type="ORF">PGT21_009619</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NC21"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1085508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="86" mass="9682" checksum="08ACE3B96FBE01A9" modified="2019-11-13" version="1">MYLAGHFLDKARNLEELTLRGLRYGPGHSTTVPGIRIVDISKPEWIEYIQKTNFSNYVKGPLSQALAYDVLGDSIFVSDGPVWKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDX3</accession>
    <name>A0A5B0NDX3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011346</name>
        <name evidence="3" type="ORF">PGTUg99_012886</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000137">
        <property type="protein sequence ID" value="KAA1129208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDX3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="131" mass="14185" checksum="D3E8DA06EE0D405E" modified="2019-11-13" version="1">MVAKAAEKSPEPSDPKKQVRLTKVTVPNKTAGPSKAVTIAVGRGAKSGCDADEQPNTSKRSRTVETDDKSEATTSSESSSKSDPEASESEDNDKSEDNNDDDRSEDEDEDKDEDEDEDEDDGSEDEVKDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFK3</accession>
    <name>A0A5B0NFK3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004456</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFK3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="56" mass="6353" checksum="8839F921066E5069" modified="2019-11-13" version="1">MLLSLIPSLPIFSCIIPIPAAQFKIWHGPDKKHHDVVYSCTRCYLADGQSGRRRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHL0</accession>
    <name>A0A5B0NHL0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_029137</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHL0"/>
    <dbReference type="OrthoDB" id="2503531at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="105"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="339"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1087329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="503" mass="56206" checksum="D2C74AD8DF9F107C" modified="2019-11-13" version="1">MPGKHPAEVHAPDSQVLQDSRELHPDSATKHNRPESQTRSQLSMNRVSKHKPPPCSPPQAAWASVGITVLKKIAEKNDQSLRNEISTLSLKHYNQEAKPRQIDAVFNLVRGRNTFLLAGTGFGKSRIPELYFRTLPIKEKAVILVLNPLDSLGDNQVLEKTWSGFTAINLTKLTICPEECEKIADGDYNFVYLSPEIFLNSKLWDRIYFSDKFQQRLGLVVVDEAHMVYEWGIVEKTHGRKRSSALGRHEDRGIFRPSYGNLGGHLMTRNNMPILLMSATCRPVAIKAIRKSLKLPEETLWMLRGELTRPEIRCIRVTMDWSMVSCNDLLDLYAPKASTPDDKVVPTIIYSGTRHRTMNVLKVLDQARGTPDEADNPDSSFAHRFHSITGSLGKVDVADDFAAGKYPIISSTMALGLGQNWSRVRSVIHMGRGDPAAICQMLGRCGRDGRPGVAIMFVEKTRVGGKNQINQIVEGVEQSDDDRMDALAITPVCLRIAFALDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NK51</accession>
    <name>A0A5B0NK51_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008573</name>
        <name evidence="3" type="ORF">PGTUg99_024300</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000139">
        <property type="protein sequence ID" value="KAA1128823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NK51"/>
    <dbReference type="OrthoDB" id="64220at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="480" mass="54011" checksum="BFD7ECB0669FD3FE" modified="2019-11-13" version="1">MSLCPKSQRLPPQKASDVPSPGRYNPLLHDPSQDPCRKDPLEIYKAPRNKESDPDPDTFGLYNSDQEHYDNIRGSRPRSRALSTPATPSKHHKELLRLETKLSEYESNITHLRETLQKSEQTTKEIRNALNKSENDYESLRSEVSRLKKQLHGAAGLHAQVIAPETEHDKGKKRRENDISNFKTELRSAETRASQSSKRNQSRMSQKDEFRLLTSFVLPAKVLQSSDSDVRSLGLPTLDLHLARLSIGHQAIAQAIPESWCAKESAIDPEQIVKALNSTIQSHREQSPASLNSLRVRLTVGPSQAVSLVTSTMPAIALTKLLVSIDDRPTTYEGEPFLLTKTTYRSHYDHTRSRHGAKYEPKEGELFDVLMFNNSRQVTETTISNIAIRRKKQLSCLDDGNSSSDPWITPHLSCGLLNGVRRQFLLGQGEITEGIIMVDDLINQGLETWEMICFNEVRGLYHVNLIDLNGNLQDGSHQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NR07</accession>
    <name>A0A5B0NR07_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023999</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NR07"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="873" mass="101055" checksum="91C77FD167ED566F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSI1</accession>
    <name>A0A5B0NSI1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Protoporphyrinogen oxidase</fullName>
            <ecNumber evidence="4 11">1.3.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HEM14_4</name>
        <name evidence="15" type="synonym">HEM14_1</name>
        <name evidence="13" type="synonym">HEM14_2</name>
        <name evidence="13" type="ORF">PGT21_008106</name>
        <name evidence="15" type="ORF">PGTUg99_000560</name>
        <name evidence="14" type="ORF">PGTUg99_023210</name>
    </gene>
    <organism evidence="14 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 17">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2</text>
            <dbReference type="Rhea" id="RHEA:25576"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="EC" id="1.3.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.4" evidence="4 11"/>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000377">
        <property type="protein sequence ID" value="KAA1092227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NSI1"/>
    <dbReference type="OrthoDB" id="438553at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000193">
        <property type="entry name" value="Protoporphyrinogen oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004572">
        <property type="entry name" value="Protoporphyrinogen_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00562">
        <property type="entry name" value="proto_IX_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF3">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="12">
        <location>
            <begin position="48"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1067520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1092227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1137704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="555" mass="61447" checksum="40254083387C460E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NU45</accession>
    <name>A0A5B0NU45_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015692</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NU45"/>
    <dbReference type="OrthoDB" id="107110at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1092837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="271" mass="30848" checksum="47633F3BE3182992" modified="2019-11-13" version="1">MLILIFIPQPPAPSLASDELLLSSLQLSRPPIDCAQYQWPEKSQATQAKDIDTERARFEILHAQHKDVTQLFDNLLEAAGLEKLREHNNQLDCVFQLSKLKQTDRGIVTFLWKDFTSGQQGDFAYLQEKLELTDEADQDKITTLIREFAGLWWRNHYKAPYTSIVGPTMSGKTRLLKELAAHVCVVYICLRDPKSTGQPPRSKIADYFLPRSSAKSGLLKHYTHLLAAILNTISKFFIGSFRIVQITMFCHIRLLDPDPADLTFAHQFGTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUT4</accession>
    <name>A0A5B0NUT4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019263</name>
        <name evidence="3" type="ORF">PGTUg99_027604</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUT4"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22278">
        <property type="entry name" value="DPBB_GH45_endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22514">
        <property type="entry name" value="EXPB1_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Expansin-like EG45" evidence="1">
        <location>
            <begin position="25"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="247" mass="26707" checksum="6BD8BDF5BF6BD11B" modified="2019-11-13" version="1">MTWMIPAAGTASLTHYDLPLNYLASCGCAYGASYHATAALSSLAYSGDERQSIGPGPGCGRCFRLTLRSAHGVDPPFVPADPRPSVVVKIVDKCPSPEFCAATRTRPNKIGQQIHFDLALPSPALNLSFFPSNVELYGYSDFGIWNIDYETVSCEEWAGWTNASSLGVERSYPGYEESCCPTNPPYTNEVCAKSIKAPPPATTYKSPAASSPPPPHPRLIFLCASAWLSLSLTSSFPLFLRLLDTHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NW35</accession>
    <name>A0A5B0NW35_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_019310</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NW35"/>
    <dbReference type="OrthoDB" id="8026949at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1092962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="86" mass="9000" checksum="03D7C4E154F6D39F" modified="2019-11-13" version="1">MTLDNTVGALEAHEVSLNGTSQSNLVSAASAKQLGCSNCGKRGHRSSNCQKKNNYKTKAGAATTVKLGGYNSGSFDDEDEIGVIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NW42</accession>
    <name>A0A5B0NW42_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017473</name>
        <name evidence="3" type="ORF">PGTUg99_017310</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NW42"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="218" mass="24521" checksum="EB0592659CD67E53" modified="2019-11-13" version="1">MSLPLTSTCFHASYGLKTFRTISLAMFISFLCLFLEKCVLSAPNPPTRNSCQSALDFEPSRVAIAEVSRGMGTSQTDKDRPIPIRIPARILYTPESFGTPRTIMERTSGKFHLFVPHLTKEQLRHIDVSVSTRRRSKLSSEEHQLLVPNLTREQRKTIAIQDHFVISDTSAEKYLFVPYLSEAQLSHCRHSLGTRDTTMEDTSDGHDLSAPVIARATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NX39</accession>
    <name>A0A5B0NX39_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Guanine nucleotide-binding protein subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GPA1_2</name>
        <name evidence="13" type="ORF">PGT21_028810</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NX39"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002307">
        <property type="entry name" value="Guanine nucleotide-binding protein G(k) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002975">
        <property type="entry name" value="Fungi_Gprotein_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF302">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-5 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01241">
        <property type="entry name" value="GPROTEINAFNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="150"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="175"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="269"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1093216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="339" mass="39031" checksum="4A35DA3887CAC749" modified="2019-11-13" version="1">MGCSGSKEISEAQQVNAQIESQIKRDKISLRNEIKMLLLGAGESGKSTILKQMKLIHEGSYTNDERDSYKEIIFSNTIQSMHVILDAMQLMQIELKDPSLIPVGQMIQSLPSQLDKTYLDPHVVTAIRQLWQDPGVRKCFERSREYQLNDSAAYYFDSIDRIGQRNYVPDDQDVLRSRVKTTGITETVFVIGELKYRMFDVGGQRSERKKWIHCFENVTAIVFLVALSEYDQMLYEDDSVNRMQEALNLFDSICNSRWFIKTSIILFLNKIDLFKQKVRYSPIQNFFPNFHPPSNPDQELWQAGADFFGNKFVSVNQSPSKQLSFNGPGGYRDRASDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZV8</accession>
    <name>A0A5B0NZV8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Autophagy-related protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ATG11_4</name>
        <name evidence="11" type="synonym">ATG11_3</name>
        <name evidence="12" type="ORF">PGT21_030267</name>
        <name evidence="11" type="ORF">PGTUg99_019984</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="11 14">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in cytoplasm to vacuole transport (Cvt), pexophagy, mitophagy and nucleophagy. Recruits mitochondria for their selective degradation via autophagy (mitophagy) during starvation. Works as scaffold proteins that recruit ATG proteins to the pre-autophagosome (PAS), the site of vesicle/autophagosome formation. Required for the Cvt vesicles completion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Preautophagosomal structure membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Vacuole membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="6">During pexophagy, accumulates in the vacuolar membrane region, where the peroxisomes contact the vacuole.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ATG11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000444">
        <property type="protein sequence ID" value="KAA1079114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="447953at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903599">
        <property type="term" value="P:positive regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019460">
        <property type="entry name" value="Atg11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10377">
        <property type="entry name" value="ATG11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="9">
        <location>
            <begin position="125"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy-related protein 11 C-terminal" evidence="10">
        <location>
            <begin position="960"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="703"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="886"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1093"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1242"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1425"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1476"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1524"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="783"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="893"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1111"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1145"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1163"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1202"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1249"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1279"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1321"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1348"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1439"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1529"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1541"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1558"/>
            <end position="1567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1079114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1094791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1586" mass="173531" checksum="41D74751111D9714" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P027</accession>
    <name>A0A5B0P027_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="9">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_023952</name>
        <name evidence="12" type="ORF">PGTUg99_027203</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="9"/>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P027"/>
    <dbReference type="OrthoDB" id="8110916at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000023">
        <property type="entry name" value="ATP-dependent (S)-NAD(P)H-hydrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 9" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="10">
        <location>
            <begin position="8"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="168"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="208"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1094523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1129063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="326" mass="35970" checksum="6E756E4C2CD2F0FE" modified="2019-11-13" version="1">MHQPHRSLLRKAFQMIPPLDGSLHKGQAGRIGIVGGSQDYTGAPFYSGYASLRLGSDLSHVICEPSASTVIKTYSPDLMVHSYLSSPKEPEAYASHQNQFEQLLDRLHVLVVGPGLGRDTEMQDWAEWTLKTAIKKKLHLVLDADALWLLVKKPDLLRGYPNAILTPNHVEFQRLLKACSIEPRDHDDDGLLAMELSKALGGCSILQKGSIDLVAREGSEVAKVSCEGSPKRCGGQGDILSGLVGTWCAWTKLYFERQSQDEKPKSHELPISPEEAWIIAAVLGSEITRTCSRLAYHKLGRSMQSSDMLGYIGEAFEQVMHGHTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4J7</accession>
    <name>A0A5B0P4J7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidate cytidylyltransferase, mitochondrial</fullName>
            <ecNumber evidence="6">2.7.7.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">CDP-diacylglycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">TAM41_1</name>
        <name evidence="19" type="ORF">PGT21_018288</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAM41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6"/>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4J7"/>
    <dbReference type="OrthoDB" id="341477at2759"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015222">
        <property type="entry name" value="Tam41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619:SF0">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09139">
        <property type="entry name" value="Tam41_Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028840">
        <property type="entry name" value="Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="17" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1096487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="412" mass="46485" checksum="6DA40659C32208A8" modified="2019-11-13" version="1">MVIRQLTRTISTKTTATSTSTSTSTSTISRIAQQPSFAKNQLIPLQSNQLSTELNSIINSFKPPIRYALAYGSAVFPQNSYQKQQQQPMLDFIFAVTHPSHWHSINLHHHPHHYSLPARLLGSPAISWLQERGPGAGVWFNVETKIHNRMIKYGVVSIDSLCDDLLDWNSLYLSGRMHKPTHILRDDGRVRLAQQVNLSSALRTALLLLPERFEEEGLYRTIAGLSYTGDVRMRWAENPAKVANIVGRQLELFRILYRPLLNALNAHLHPVSQDPMSDSLRVFHQDPSPAARAQLLLKLPLGLKDRIRATYDQRWNLDRAFANHHQHHPDSLISPTTSSSSDELEIWTRIAADQKLRQVIQSALIQIVAKPSLYQSAKGILSAGPIKSLRYATNKEMQVEGPVPILCHHCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4V2</accession>
    <name>A0A5B0P4V2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018850</name>
        <name evidence="3" type="ORF">PGTUg99_033864</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4V2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137646" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="60" mass="6437" checksum="86B333B462E0DFA0" modified="2019-11-13" version="1">MNYLLAIVACVLTLINTLRAAPASVGSGVLRPKHTHATRRVIGYNPPTRQLPDQPIPGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8V6</accession>
    <name>A0A5B0P8V6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026439</name>
        <name evidence="3" type="ORF">PGTUg99_034421</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8V6"/>
    <dbReference type="OrthoDB" id="10615786at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="356" mass="40206" checksum="52425E05B9B8C0FD" modified="2019-11-13" version="1">MKTTTLTEQSRDAALGRAPSYWHPYAIPSENRFIKQAQKAQEQKASAPGRNRGQKQSQRGQKSVFKWITNIFRKNRRGNSKDETAPGTSGQTAGSADKGTASPMVQLFNDLFQKDKKPEAHFKLAQEAVEYLLRTPGVRQEELSEWVPKLIDVGSENGHGHNLEFRSTLFYGLHTLVLKHYANDVLRRSILSQLNKALEGIRTDFGPAIVSEWDKELYNPSSGLSKKIGNWKQLKDIITYEPSLKDLRIGLNNKNKASIIQNLDYIKTIFAISTMRNYSAETRVASIGVLSEIIKQENSEHIKGHAALELALSLKDPENTEVFLFDHEKKLLESLTAQSNLRTSKSPKRLDFLEVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P9P7</accession>
    <name>A0A5B0P9P7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002638</name>
        <name evidence="2" type="ORF">PGTUg99_035383</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P9P7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1134339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="77" mass="8405" checksum="7DC019F8CE22D902" modified="2019-11-13" version="1">MDIHIATSRPRAPGGQPCRSIWLVSLARLSLSIQSHLLVPKTHRTNQTDSEKADQSSTAPLNLHGLAATAEFFVELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAV5</accession>
    <name>A0A5B0PAV5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAV5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="91" mass="10113" checksum="FE2560F87E190948" modified="2019-11-13" version="1">MFQRRIIELRLILDGSQSSGHHTGSQQSNQFDPRDASQGHRRILGREKKTAVEFSSAPLNLPITTGESSCCTFKAQHIDVHVDTMHSGSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PE34</accession>
    <name>A0A5B0PE34_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PE34"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="140" mass="15480" checksum="DFDC6A990D181DF2" modified="2019-11-13" version="1">MGSFGSLLANLVGSKADRLHLITLGLHIDSTKLNLQFRQPTRFRINPQDSSIFSFQDQYSSFLNLLVSGSNLISHLTSRFRNASISLGAPYSVFFVGLTVDVGSIELAVGTQLDPLIFFVLRGLWSFVELTRRLNAQSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PFA2</accession>
    <name>A0A5B0PFA2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4218 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PFA2"/>
    <dbReference type="OrthoDB" id="2504147at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004242">
        <property type="entry name" value="Transposase_21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579:SF1">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02992">
        <property type="entry name" value="Transposase_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="983" mass="110964" checksum="ADB31EE638929667" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PG95</accession>
    <name>A0A5B0PG95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028576</name>
        <name evidence="3" type="ORF">PGTUg99_014961</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000144">
        <property type="protein sequence ID" value="KAA1128137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PG95"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137771" evidence="1">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="51" mass="5560" checksum="33996A1559EC3D7A" modified="2019-11-13" version="1">MYIPLVFLLGSLMFFQITTVSAFPVEALASNGFHVDCANDPNQCCPACKPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIL3</accession>
    <name>A0A5B0PIL3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIL3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1099739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="92" mass="9718" checksum="415FE97DB84D7E41" modified="2019-11-13" version="1">MLELGANAIDARPSAPSTLIVLGIREVTIERQNMAKLAAKWLGRPSAQPFGDVCLTDRSLSLGPPDGRMGQSNAHAHLSGFYSCVSSHDVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PPC4</accession>
    <name>A0A5B0PPC4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000044">
        <property type="protein sequence ID" value="KAA1102490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PPC4"/>
    <dbReference type="OrthoDB" id="2505887at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="224" mass="25054" checksum="4D1EB1CA52442E78" modified="2019-11-13" version="1">MSFTGFETLTDADELLRRCLEQHHRGNQITIHASDSSENSSNSTTTNDPDYDLEIPGEPILCKAKLKGEYWPASLIGCVGLRNAKSRTSRLDPVSLSEKYYSVHFFDGTSAEVPRSFFLTSIERNFHTVPVGRIQTIETTYKQFLPKLIKILPDVDAILAGNASDSGVKQRHEDFLGGQRGQDCIPNEDIIYGKYSDSLLYEVAAFLSNRYSQNVRSDTFSGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQE7</accession>
    <name>A0A5B0PQE7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RET1_3</name>
        <name evidence="9" type="ORF">PGT21_004548</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQE7"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="8">
        <location>
            <begin position="171"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1103917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="502" mass="56070" checksum="8CF7993CE9E3F6A6" modified="2019-11-13" version="1">MPPKRVFKPIRPNNNNNNNNNSTTSTSNTAAPAPENHHQNDQPPPPILLEELESLLNQSTTNSSEQHLDTKPDISQIDHQNISQPKPAPLFRPLSPEPAPLPQKKNLQPPNIHQQQQQQHPKKSSKNYPKKTHQHQSNVDNELWLSKNLTDPVKTVEDKWQLLPAFLKVKGLVKQHIDSFNYFVDVDLKAILRANATVISDVDPNFHLSYLDINVGEPSRLDTGSATNKRITPHECRLRDITYSAPISVTIQYTKGNKRVLSKGIPIGRIPIMLRSNKCVLTGKSDAELAKMTECPLDPGGYFVVKGTEKVILVQEQLSKNRILIETDSRKGTIMASVTSSTHERKSKTYVVTKNSKVYLKHNSIQEEIPIAIALKAYGITSDREVVQIVCGNNDDYRAAFSICMEHCHEAGVFTQKQALAYIGKSVKMVKKGPVAGPKRPLTEEALEVLSTVVLAHVPADNLNLQPKAIYLAVMARRVVQAMANPKLVDDRDYVGNKRLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQQ7</accession>
    <name>A0A5B0PQQ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQQ7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="57" mass="6325" checksum="2502239FD2297FAF" modified="2019-11-13" version="1">MGRLDVRGQSIRRLKGIGSNLCAKTFSAMMAIRGDPRIGNRRYRPDIRDAIAPALVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRC6</accession>
    <name>A0A5B0PRC6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRC6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="379" mass="40956" checksum="4EBEAE2BAA83E869" modified="2019-11-13" version="1">MREALLGPHPGFRPPTDASHWLELKPTTPGDWVRRGLLFEDSGRTFDGSSWLLLVLGVIVIYTSRKGADEGIKNAPFNLSFPFSFLNQCPMHRPSTPYPIPARTLSERSTMTEDYSSDFPVPNASPAAPSSPEAMSMDLDVIVEAPPSHSIPAPAPISSPRAVPAFRIRGAANAAPHSSPTPLVPVNQNGGGARARGRARNRGQARGRGLDPVRVRGRGRGFVPAPLRGRGRPSGSNSLPTGPVISNRAVVGRGATPPPAPRGSRLPTPQLSPGGPFEQNGSVTSADNSWVEPHVRTRTRVPSPVPFGFYDVDDYHRLAPHQVAALLDRCPDFGRYDQYRFAPQLYTAFYQEVASALVGHFTRHPAANALEREGRVALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT01</accession>
    <name>A0A5B0PT01_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002025</name>
        <name evidence="1" type="ORF">PGTUg99_010471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT01"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="73" mass="8570" checksum="3B0BA2417B234246" modified="2019-11-13" version="1">MINPCSICSTFFDCGKNTTRISQNQQYQMVLRYSDLVTQWKEVSVNLHKSCTNYNKHIDERVCNFVSMTCRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTL8</accession>
    <name>A0A5B0PTL8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PTL8"/>
    <dbReference type="OrthoDB" id="3363836at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1104032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="163" mass="18025" checksum="B25F2B5A23AA8C53" modified="2019-11-13" version="1">MFYSPNQVVEAFSTATLEWDPHCFDSANNIDIYLQSLDVAQPIHVSSSPPPTCPYGPIFFAHFNGSIPASAPTSAHDGKQLGGPSIEYITNLFHRSHHALSGENNNNKEQLSVPLFRRPRLRSTASCLHSALSTGASTHQSSNMIISRMEYSHNNQLLEPEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PU52</accession>
    <name>A0A5B0PU52_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023144</name>
        <name evidence="2" type="ORF">PGTUg99_027701</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PU52"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="95" mass="10795" checksum="D3430BC593A4DA48" modified="2019-11-13" version="1">MPEEQQYFSPRGTKTEIHSNQLPGFDEEQTRSGSSSFCSDTCALVECLDQERATLKPSLQQPRQLQRVFWFGGVGMASNWGHEQVLQQAKFRQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PWZ1</accession>
    <name>A0A5B0PWZ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PWZ1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="163" mass="18537" checksum="F3694002EEE8F3EC" modified="2019-11-13" version="1">MSIEPLFATIHSYLSRKRVNELLSKLWTERGMDKLCFLSRPRTKTNGRFIKDFQLRLVDLLLVKSTPVTFRDHPGKIDFLRPVSSRILEIHSGIKECLREIVKLYGAPCQLIGTFSFALRASSRILLESNPALHTAISEISAIRLTRVDLCDLYLIGALAQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PY85</accession>
    <name>A0A5B0PY85_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Prefoldin subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_023355</name>
        <name evidence="5" type="ORF">PGT21_025379</name>
        <name evidence="7" type="ORF">PGTUg99_010457</name>
        <name evidence="6" type="ORF">PGTUg99_028216</name>
    </gene>
    <organism evidence="4 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1121034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000169">
        <property type="protein sequence ID" value="KAA1127033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PY85"/>
    <dbReference type="OrthoDB" id="2015447at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="86"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1105891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1114853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1121034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1127033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14684" checksum="8A9064FFCA275B0C" modified="2019-11-13" version="1">MSNDQQNDEIRNILGQLQRQARDVTRELTRCRAELGHKVRVARQSSLTLTELNKLATGDIRFYQPVGKMFMLKSRTSIDDGLKTKQKSAEDDVNKLITRIKSLEAEVEANQRALKEIVRSIDMESRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYJ2</accession>
    <name>A0A5B0PYJ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cu/Pi carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PIC2_3</name>
        <name evidence="14" type="synonym">PIC2_5</name>
        <name evidence="13" type="ORF">PGT21_023771</name>
        <name evidence="14" type="ORF">PGTUg99_027262</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transport of phosphate groups from the cytosol to the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1121018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYJ2"/>
    <dbReference type="OMA" id="YKGAIWL"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035434">
        <property type="term" value="P:copper ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000076">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000131">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="91"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="188"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="289"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1105907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1121018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="386" mass="40763" checksum="67105BDD86F59A9B" modified="2019-11-13" version="1">MSLLFPNMASLANSLTPSVGGLNTPFPSLKVASIVDDAKSKAKQLESKSKEGIEGAKAQISSEAEALRSKVASAAGVGAASKASIELYSTKYYAACAGGGVLACGLTHALVTPLDLVKCRRQVDKTLYKGNMDGWSKIYKSGGLGGLYTGIGPTWVGYSVQGACKYGFYEYFKKKYADAVGPANAVKYKDAIYLAGSASAELIADAAYVPLEAVKVRMQTTIPPFATGMVDGFKKFVAVEGTGGLYKSLGSLWSRQIPYTMMKFWSFEATVTKIYASLGKPKDSYNKLQQLGVSFVGGYIAGVFCAVVSHPADTMVSKLNAVGKEGSAKPSVGSIYKEIGFGGLWAGLGTRIVMIGTLTALQWLIYDYVKVAFGLPTTGSAEPQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZD1</accession>
    <name>A0A5B0PZD1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Catalase immune-responsive domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZD1"/>
    <dbReference type="OrthoDB" id="2512064at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase immune-responsive" evidence="1">
        <location>
            <begin position="130"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="228" mass="25433" checksum="B9673AE26CE37289" modified="2019-11-13" version="1">MKRQIITDHVHDHYCRRFGAASSGETSELSCSSRSKASTAFTNSGFIFAAIRSLPINTILACLDRLEAHIQDFLIYHALTHHRFLGKLRIQFQDISHESDTFAMKNVNAFDFAPFLKMSLVQLLVTTLKSNFKPARALWSKVFDDGAKEYFVKNVSGHLGNVSLDRIKTDQVAIFLAVDKDSGNRVAKAISLKDTPEPYKPKPPAQATKFAPNLKSSGQMIFENSIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZW8</accession>
    <name>A0A5B0PZW8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034084</name>
        <name evidence="3" type="ORF">PGTUg99_033179</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZW8"/>
    <dbReference type="OrthoDB" id="2497945at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="535" mass="59939" checksum="95E5A4811B18BDF0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q4I0</accession>
    <name>A0A5B0Q4I0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q4I0"/>
    <dbReference type="OrthoDB" id="2507562at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="172" mass="19262" checksum="F443A98338531FE4" modified="2019-11-13" version="1">METGCGLTEEEEEWLDNGGNLIEEFVLMDRLKEISPNNQHIAISATDIVIIEKINTYEIDKPQAKPSKTTSSPQPKSKTAVASPIAKKKTPVATPSAKKKESVKEKLNLKSASKASRKPVRATLAQKLEVLDWHRKNGENQTKTARHFAEIYPEIGFKQPLVSAWVKEEDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9D7</accession>
    <name>A0A5B0Q9D7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033965</name>
        <name evidence="4" type="ORF">PGTUg99_021088</name>
        <name evidence="3" type="ORF">PGTUg99_035464</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000106">
        <property type="protein sequence ID" value="KAA1130800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9D7"/>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1130800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="178" mass="20378" checksum="D50E0815147977EF" modified="2019-11-13" version="1">MLQDKGNANRNSQSEDLGNQTNGWDCSESWADGERFLFEMAKQISDENSEWSNRLLVHNPSILISNSIRDILLQFFTAPSIHVSFKFILDLAEQESSIPAPKPKQDFNKRRSRMRSFDYQKYDGRSNSGTGSKPQPKFPNRDCPVLPLRACIEQQAEQDVPDEFISIPRISFSCCDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBD3</accession>
    <name>A0A5B0QBD3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBD3"/>
    <dbReference type="OrthoDB" id="2506573at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="229" mass="26136" checksum="A3B22DD703FA02D6" modified="2019-11-13" version="1">MGSHAKHSNKTSFSLSVKHLVNNRKLLRPNLAKRSANPAPVIPSSSSNDVEAWPSGSDDDEPHHPSCYNMHRRTAAEIEVMRKTQVNQISQIDKILRVINEMNDRMTPDEDIPVGKSQRTPVQRSDLRKLVPATLCDTLRVVHPIQDFPRPATEGERREWGHLIRPSSTQETNRYVHEVATPQQFLWDLALDIFVELMKCGEYAGVDMDHQDPQIIASEMRKYVRETLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCY3</accession>
    <name>A0A5B0QCY3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome activator PA28 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_034670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCY3"/>
    <dbReference type="OMA" id="CGPIYSN"/>
    <dbReference type="OrthoDB" id="6591885at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008537">
        <property type="term" value="C:proteasome activator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.180:FF:000002">
        <property type="entry name" value="Proteasome activator complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.180">
        <property type="entry name" value="Proteasome activator pa28, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003186">
        <property type="entry name" value="PA28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036997">
        <property type="entry name" value="PA28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009077">
        <property type="entry name" value="Proteasome_activ_PA28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036252">
        <property type="entry name" value="Proteasome_activ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660:SF2">
        <property type="entry name" value="LD45860P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660">
        <property type="entry name" value="PROTEASOME REGULATOR PA28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02252">
        <property type="entry name" value="PA28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47216">
        <property type="entry name" value="Proteasome activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome activator PA28 C-terminal" evidence="4">
        <location>
            <begin position="111"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="63"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1110942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="256" mass="28787" checksum="D029BDE2A28DBD36" modified="2019-11-13" version="1">MATTNPLWSEATDKAVQTQLKNWTQEIEQKGKEAIHVRLPAKILEINQVIEDFSDSTLGHLVATEPTLSSPPDEPDNNTGRKKRKTANGKEAQPPPTDQPTPGGVKNPAHVASNPKVNAHFIFLRTHWEELINITNAIKIHINLLIPKIEDGDTFGVQVQEECLSELSRAQDSGYNLLEAQLKHNMSRAKLASKLIKYPNVEDYQLALQEHDRKSIFLCRQHLLDLRNIYALLTDLLHKNIVKITKPKGANSDGMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEE5</accession>
    <name>A0A5B0QEE5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEE5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="379" mass="42627" checksum="7DB453DCA5192B79" modified="2019-11-13" version="1">MGRRENRKRPLSLGQRDYWIEEDGNDERRQITSKRRRLDLNLEPPSPEPAINPLQGKDSRGKYQDLNLNLHPPISSEGSDSSAGSSRSQFQPSGKEATQMPPVEAGPSRKKTLTLDQQNYWTEGENGDDNSTQPASKKRRLGLNLQSYSLESVLDTTCGQGSRGKYQDWNLNLDPSTSGEDSDSLPAPSRPEIPPDYVNPVSSQADPVIGSESRVEIPYPIVLYENSVDNVPEEPNSSKNGSRSSPAQLEPAGQLHGANSRSPDPESHFRNTLIIGIDTPHGSEESGGSGDEEVLIREFYVENLNPKIWPWISIIWQRVQKSIFRELEKSVDPLIQDFIDEYLGHPDYGINYQKTNIERNVREEAHRLKNQLREIHQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QG88</accession>
    <name>A0A5B0QG88_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017872</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QG88"/>
    <dbReference type="OrthoDB" id="10289240at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="193" mass="20580" checksum="23F779C993D3A083" modified="2019-11-13" version="1">MPFNTDWIPGATFSGGVFSPGKVDLISTANPNLLSNVDTARLNDGDSIDNSSLASDDKTNIARSDADPVIKTTSAIKHAPSASPPAYCLEVLPLGSPENPIKIEDKEANTDGRNNDGINDYERLNSPRFNPVEIIPFQATVIDEEEVIRAVRLKSGCETLLLNGEGDGGVASSALLCYQNNKTTKLSLAVYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QH00</accession>
    <name>A0A5B0QH00_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023335</name>
        <name evidence="3" type="ORF">PGTUg99_013642</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000277">
        <property type="protein sequence ID" value="KAA1112461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QH00"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="103" mass="10889" checksum="8AC8FABA88492A97" modified="2019-11-13" version="1">MREKCRRGPALGKASLAKASESEGPEARLRSPADREVPRGAGTNPVSTDSPPRGSPMYVQRAHTTQASREAQLSPRRVATRPGVAVSLCSQSNSETASSDLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHM9</accession>
    <name>A0A5B0QHM9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007405</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHM9"/>
    <dbReference type="OrthoDB" id="2508706at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022912518" evidence="2">
        <location>
            <begin position="17"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="546" mass="57588" checksum="631417CAB5EEB166" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHP6</accession>
    <name>A0A5B0QHP6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA-directed RNA polymerase III subunit rpc25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RPC25_1</name>
        <name evidence="9" type="ORF">PGT21_006343</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHP6"/>
    <dbReference type="OrthoDB" id="10256606at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04330">
        <property type="entry name" value="RNAP_III_Rpc25_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013238">
        <property type="entry name" value="RNA_pol_III_Rbc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08292">
        <property type="entry name" value="RNA_pol_Rbc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="7">
        <location>
            <begin position="78"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III subunit Rpc25" evidence="8">
        <location>
            <begin position="154"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="248"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1112692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="308" mass="34901" checksum="8C7CF4F8B1CFD979" modified="2019-11-13" version="1">MYRRPPKGLAYIHIHRSLAYIECDPSSKEKKTIKIEALSFFEKKVTYNRREQSGKKTEAKRKGSRKAGWKMFVLSHLKDTVRVQARDFGRDRASVITAEINRKYSNKVLPDLGLCIALFDLLDASEGKVLYGDGCLYHRVEFTLVVFRPFIGEHMIGTVKSSTPSGVRVSLKFFDDIHIPCRLLQSPSCYDPTLNEFFWASEYSDPEAASTMPADLLSISEDQRLYMQIGQPIRIRIEEEHFLDVGPKEAPKAANPTPEGVEPSALEEQLRKEKEEGIPPYSLVASCDGPGLGIIAWWQSEEDGQENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJU1</accession>
    <name>A0A5B0QJU1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJU1"/>
    <dbReference type="OrthoDB" id="2504984at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="532" mass="60115" checksum="52A3279FB232031F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QKY4</accession>
    <name>A0A5B0QKY4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024314</name>
        <name evidence="2" type="ORF">PGTUg99_016513</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QKY4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="152" mass="16170" checksum="D58D727AC0FD8768" modified="2019-11-13" version="1">MTHQGASGTQGCTGAPRPVRAALWVSMVSLRRDRVDPQEARPGGDWGGRLGVDMAVGSSSLCHGDDTDRGAMRPGSSGTVYGWGGVILATRVKGRSSSTSTTATELNFCCLLQKTNQPQRVSFRSQLNLSRSHLKAALKAPSLQPTAYRLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QLW3</accession>
    <name>A0A5B0QLW3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QLW3"/>
    <dbReference type="OrthoDB" id="2506799at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="737" mass="84813" checksum="926D3FEE7E94C0F6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QM01</accession>
    <name>A0A5B0QM01_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_037176</name>
        <name evidence="10" type="ORF">PGTUg99_024820</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000273">
        <property type="protein sequence ID" value="KAA1114152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QM01"/>
    <dbReference type="OrthoDB" id="204405at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004538">
        <property type="entry name" value="PRK05888.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004539">
        <property type="entry name" value="PRK05888.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF20">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="152"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1114152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1120121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="260" mass="29463" checksum="B326E4459AD41538" modified="2019-11-13" version="1">MLSLTAKRWTTTTTTTATKTSSLRLNNSRAFRSSPRNQLAHQAPVKEIDPIPAPTQTPDLPPFHFGAPSSSKDQVEPPEFNYKDPDYKPIVYKDYSNGPSALDKAAKLFFLTEIARGMWVVLGQMFRPPYTIMYPFEKGPVSPRFRGEHALRRYASGEERCIACKLCEAICPAQAITIESETREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVESQNTEYSTETREELLYNKEKLLANGDRMESEIAANLHADHVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNI4</accession>
    <name>A0A5B0QNI4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">(2E,6E)-farnesyl diphosphate synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Dimethylallyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Geranyltranstransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">COQ1_2</name>
        <name evidence="12" type="ORF">PGT21_025578</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNI4"/>
    <dbReference type="OrthoDB" id="9927103at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1114856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="452" mass="49570" checksum="11020CFBAE97E06C" modified="2019-11-13" version="1">MALRLPPLLLLPRLTTRQLSTQPKTKLAPSLSILDLPSTTTHSLQAIVDPFELLANELSHLKQNIKALVGSDHLRLNSIAKYYLASAHQGKHLRPLVVLLISQATAAPPLADRPRPTNALHFIDRPISPPEILNDNNSSSDQQGLLPEEAEDSAILPAQRRLAEIAELIHVSSLLHDDVIDQAETRRGQASAPRQFGSKLSVLAGDFLLARASLNLARLRNFEVIELISSVISNLVEGELIQLESILVPHLQNPPKLAPSSDPSNLQSTSLAHSPAAFDPRLFDFYMRKNYLKTASLIAKTCRAAVILSSSSSSPPSPQTQELIEASYQFGKHLGLAFQIVDDILDYTGSEDELGKTGNGSDLKGGLITGPGLFAWKQFDRTFGELVVRKFCRPGDLDLAKEMVHKSDAIRCSYQLASQHIQLCRSQLNSFNDSDAKAGLQRLCDLVLNRNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPS8</accession>
    <name>A0A5B0QPS8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Domain of unknown function at the cortex 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QPS8"/>
    <dbReference type="OrthoDB" id="2504989at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013897">
        <property type="entry name" value="Duc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826:SF1">
        <property type="entry name" value="UPF0590 PROTEIN C594.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08588">
        <property type="entry name" value="Duc1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Domain of unknown function at the cortex 1" evidence="2">
        <location>
            <begin position="4"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Domain of unknown function at the cortex 1" evidence="2">
        <location>
            <begin position="745"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="574"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="620"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="875" mass="96471" checksum="568F1251352153E7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ77</accession>
    <name>A0A5B0QQ77_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ77"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022864056" evidence="1">
        <location>
            <begin position="17"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="62" mass="6597" checksum="100FE697EE3824EC" modified="2019-11-13" version="1">MHSRFMLAGLCSISTTFDGVIGPAIEGGAERGGIGLSHTTNYPTKHPQLHMLDRTLLVMGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWE8</accession>
    <name>A0A5B0QWE8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016801</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWE8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022809521" evidence="2">
        <location>
            <begin position="34"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="99" mass="10715" checksum="D3B81ECAD9A74FB6" modified="2019-11-13" version="1">MWPCSSPLFISNRAGYVALFLIFFHLAPLPSGASLPSPHPPPVTPLSPPLTPFLPSEPPLNHLPEPSGHARGLQGAPSERLIIQTINRSPERLIKRSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWF4</accession>
    <name>A0A5B0QWF4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015050</name>
        <name evidence="3" type="ORF">PGTUg99_001722</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000246">
        <property type="protein sequence ID" value="KAA1118611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWF4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="61" mass="6830" checksum="1B6C5118A98973B6" modified="2019-11-13" version="1">MRPDTATSSVNSQNKSIWLDRAGKRVGSFKSSKLHNTSKKHVPVTQQDRFSESIQSNIGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX56</accession>
    <name>A0A5B0QX56_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_026487</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QX56"/>
    <dbReference type="OrthoDB" id="7700931at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF122">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023020355" description="FAS1 domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="3">
        <location>
            <begin position="415"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1117886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="653" mass="72360" checksum="4AA09730275702FD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0V9</accession>
    <name>A0A5B0R0V9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_002260</name>
        <name evidence="6" type="ORF">PGT21_002437</name>
        <name evidence="3" type="ORF">PGT21_030197</name>
        <name evidence="4" type="ORF">PGT21_030312</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0V9"/>
    <dbReference type="OrthoDB" id="2509741at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1101794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1118685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1118697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="219" mass="23894" checksum="CB36CBB4AE2165F2" modified="2019-11-13" version="1">MNDSPNSQEVARISREIEKLQRQQRQADQEVRRIAAQFRADLLEMNLQNINRQSTMAERIRILENRVAEAISPNGPNAEVNTREQTAVVDWNAGRVLDGATRPDDPIGPQGYDENDVNLVPALMTLTRGRTRQDGTNSGGGTVSIAERYRAMMDPFGALPRQHTDTHATPPQGNSGNHSPDEVLVDQGQGSSPGSSARAPRSLGGAMRADDQDMEMGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1A4</accession>
    <name>A0A5B0R1A4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1A4"/>
    <dbReference type="OrthoDB" id="2509823at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="445" mass="50764" checksum="10537626FA6B31FF" modified="2019-11-13" version="1">MDSTTVEYQPHLISLFCEIIDLAGYRRITPRGRDRDIAEKFLSSREIHAGKALLVEVQTRSLPALQQQLHGLMQSIATGRLKKQQSNPKVTDALEFIRQLGATLNLIDSSLASLASAPISEELGFTCKVDHEYGILKRYKCIGLVQKFDRFLSDTFADLSGDYCSLMNLWYSAAYFPVRIMRRMLKPGAPRLQRKIREKTDEACRIIDAIIRSPIRSDFSFLQESWQDDVDRLSACLNDLTRHVDHSVRFGESDTTDDQDDLSNTSSEEFTKELHESTPEFGTDSNDPTTSSQENVLLNLDLMPRIRRAIPLLKLGRTFFSKLLDTPESKTPFTLSPRVSSYDMGCLKKETSSLCAHILKIVKSTYQPENEDELHNEMAKISVWSKELSRHFDSSMMLFAFFLIPAPSRIDPPFPEDLLRSSFFEFRCQFHLALEQLLAADRNIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2Z1</accession>
    <name>A0A5B0R2Z1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2Z1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11709" checksum="71BA908977900DEA" modified="2019-11-13" version="1">MELLQHIFTDLVILHKRWPRSPGPPPTTTLRLVCRQWANWLYEKELYRTLCFTTRTKTLAFMIIDYMSEISPRLCRAGEARGVKCVYLHHADMSVIMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3A3</accession>
    <name>A0A5B0R3A3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-zipper-like transcriptional regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LZTR1_30</name>
        <name evidence="5" type="ORF">PGT21_033312</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3A3"/>
    <dbReference type="OrthoDB" id="2496273at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051568">
        <property type="entry name" value="LZTR1/Attractin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376:SF1">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="4">
        <location>
            <begin position="474"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="369"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1119753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="744" mass="84775" checksum="C053540783A8737B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3N0</accession>
    <name>A0A5B0R3N0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_037172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3N0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1120117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="53" mass="5910" checksum="D8BBD7ADC79382B4" modified="2019-11-13" version="1">MDAKDSLYMPFLEDCDSVPREIEEKCCSEGQYTSAGKYGQLTTMPTPSICILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R9Z1</accession>
    <name>A0A5B0R9Z1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cryptic loci regulator 2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_015057</name>
        <name evidence="5" type="ORF">PGTUg99_037787</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R9Z1"/>
    <dbReference type="OrthoDB" id="2421327at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070824">
        <property type="term" value="C:SHREC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038986">
        <property type="entry name" value="Clr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031915">
        <property type="entry name" value="Clr2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018839">
        <property type="entry name" value="Tscrpt-silencing_Clr2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38046">
        <property type="entry name" value="CRYPTIC LOCI REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38046:SF1">
        <property type="entry name" value="CRYPTIC LOCI REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10383">
        <property type="entry name" value="Clr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16761">
        <property type="entry name" value="Clr2_transil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cryptic loci regulator 2 N-terminal" evidence="3">
        <location>
            <begin position="102"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Cryptic loci regulator 2 C-terminal" evidence="2">
        <location>
            <begin position="452"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="687"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="691"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="712"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1122556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="732" mass="83481" checksum="4B405C2F1D4971E5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RLK3</accession>
    <name>A0A5B0RLK3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM complex subunit LSM4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LSM4_1</name>
        <name evidence="9" type="synonym">LSM4</name>
        <name evidence="12" type="synonym">LSM4_2</name>
        <name evidence="12" type="ORF">PGT21_019971</name>
        <name evidence="13" type="ORF">PGTUg99_004129</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000173">
        <property type="protein sequence ID" value="KAA1125978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RLK3"/>
    <dbReference type="OrthoDB" id="747253at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097525">
        <property type="term" value="C:spliceosomal snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01723">
        <property type="entry name" value="LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000024">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034101">
        <property type="entry name" value="Lsm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="82"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1097791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1125978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="149" mass="16304" checksum="68354182C06883B8" modified="2019-11-13" version="1">MLPLALLHGAQGKPMLVELKNGETFNGHLIQCDNFMNLTMREVYQTSANGEQFWKLPECYIRGNTIKYIRVTDNVIDQVKQQEDAYRQQNRSSRGGGNQTNERGGRGGSGGRGYSNSNNRGGPGSNRGGGGRGNSDRGRGRGSDRGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RLK4</accession>
    <name>A0A5B0RLK4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010870</name>
        <name evidence="3" type="ORF">PGTUg99_001612</name>
        <name evidence="2" type="ORF">PGTUg99_003963</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000293">
        <property type="protein sequence ID" value="KAA1111259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RLK4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="193" mass="21427" checksum="E2EBBBE628BEA96A" modified="2019-11-13" version="1">MTTPSRPPQSPPGCACCGSTRSRLSDTMLTQSAALTGLGAPVQPRVPLAPWCVMWSPFRPGRRHGGWDFFDPSRCVVALLWCCKPVWALAQGPRVQVSPGLGPLPRGLELEVRAWPGSWHKPKSGLAEPKLWAHQNHQPQNLTRPPHPTPTSTTHWFWPGWHSRLGSAKLGFGPTHPNFGRCQARLEYRPSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RNL0</accession>
    <name>A0A5B0RNL0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucosidase 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glucosidase II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_012356</name>
        <name evidence="16" type="ORF">PGTUg99_014221</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="16 18">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; N-glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3237269at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090599">
        <property type="term" value="F:alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06603">
        <property type="entry name" value="GH31_GANC_GANAB_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033403">
        <property type="entry name" value="DUF5110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036607">
        <property type="entry name" value="PRKCSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028146">
        <property type="entry name" value="PRKCSH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF54">
        <property type="entry name" value="BCDNA.GH04962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17137">
        <property type="entry name" value="DUF5110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12999">
        <property type="entry name" value="PRKCSH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13015">
        <property type="entry name" value="PRKCSH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="MRH" evidence="14">
        <location>
            <begin position="1400"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1337"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1138"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1337"/>
            <end position="1352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1076583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1126748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1525" mass="171983" checksum="715283BE8D8E6F03" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RXA1</accession>
    <name>A0A5B0RXA1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032837</name>
        <name evidence="3" type="ORF">PGTUg99_013017</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000111">
        <property type="protein sequence ID" value="KAA1130227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RXA1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="101" mass="10915" checksum="D518C6D61BBFFAF9" modified="2019-11-13" version="1">MVVARQRPRSHESPVLNPKDSKHVDTWVQDGSHKTVKLVRKNYNCVISLNPPSSSEFTTAPESGLSSSASISTSSSINAQTTSLDQNQPTNSRPSPVLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1G0</accession>
    <name>A0A5B0S1G0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ras-GEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020372</name>
        <name evidence="4" type="ORF">PGTUg99_032051</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1131880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1G0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366660" description="Ras-GEF domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="70341" checksum="81FFD5B3B71A8C75" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SC63</accession>
    <name>A0A5B0SC63_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020303</name>
        <name evidence="2" type="ORF">PGTUg99_012504</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000041">
        <property type="protein sequence ID" value="KAA1135105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SC63"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1135105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="109" mass="12176" checksum="76F2291020825B95" modified="2019-11-13" version="1">MNYSSKALIFAVLLCGDINQKKVAAVNMSKCKDCGSTNISLGQPRQEACQMKTICQQYAHPHNPCQKNVIWKVPVTCHEDTCLAVNDHFDCEESSNYHTFSVCPTNGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SHY2</accession>
    <name>A0A5B0SHY2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016481</name>
        <name evidence="3" type="ORF">PGTUg99_031239</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SHY2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1136114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="52" mass="5687" checksum="86EE888B073D9C68" modified="2019-11-13" version="1">MNSSSSSDVKEPSRKELGSNSAANKKNEAPVKGAENESVSFLKDKSKKRLYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SKA1</accession>
    <name>A0A5B0SKA1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024603</name>
        <name evidence="2" type="ORF">PGTUg99_010445</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000013">
        <property type="protein sequence ID" value="KAA1137034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SKA1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1137034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="68" mass="7619" checksum="5BBA5E8534744933" modified="2019-11-13" version="1">MLNWVSTKTRQGATRLALTVVILIVKMGGGQIERAVMMTDRALHNQRVGFQSARGWTCVKFALIYQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SKC5</accession>
    <name>A0A5B0SKC5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025332</name>
        <name evidence="3" type="ORF">PGTUg99_013872</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000006">
        <property type="protein sequence ID" value="KAA1137623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SKC5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="63" mass="7024" checksum="977579F7153573BC" modified="2019-11-13" version="1">MLLNNGGEIPGRGAERLFNRSSSNPQKQYTVTLDFGGNYRGCRQRLNDIKDGGVQVNVKLKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKD0</accession>
    <name>A0A5B0LKD0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_000870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000198">
        <property type="protein sequence ID" value="KAA1064098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKD0"/>
    <dbReference type="OrthoDB" id="2499742at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="88"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1064098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="345" mass="40631" checksum="2F78310384228216" modified="2019-11-13" version="1">MDVRRQLDERERARNKWKRKKVRTKKPKSPPILPRNPTTSEIEHAASVVNRDFKLYDHGHVRVFDKAKKNKLIADINFTDIKKMRKRELMDLKFLCSYLNEAKLFVNPVHSKGRSCGGVMWAIGWRKSMKRYEIIGRYKNKKNIEKYNTIYRRHISQQKRAGQILWNLFHKVGDVALENNRQYMRENNLPGWADNDFHEENDSHHAFASNLTFTSHGFFNHAHKDEGDDSELPFAFLLCVPTFRGTGELAFKTDGYDVRGGHFIFRDYGFGVQFKPDMICQMIFSQRDYIHGTLQPIESSKFTKLGMSLQISKKITNICKRIKAREFDEFPSMYYVALNSSYLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKZ2</accession>
    <name>A0A5B0LKZ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002773</name>
        <name evidence="3" type="ORF">PGTUg99_014846</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1079968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKZ2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="237" mass="26420" checksum="4F05F84FEC393EAD" modified="2019-11-13" version="1">MQGLQRAVVLARLPGQGLRKHVASGHLPTYIGIVEGTALAWFPTMALHTPASHGGVLRQKLERTGRHPLNKVTRACLRGGLDGHFWWGFSRPQEPPVWGDLSVKIPFQLAFESTYTAGQKCENRNVQHDRREKGSSSGNLGERGFKSPRNSRMTVFTLRLVWPSMIVIIDHGEYIGGAQLKTGPHDGVWEATSYPLFLLRKCLESSNLDFDLTIGDDGLLRSWTAKRGLNNIRLSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LL40</accession>
    <name>A0A5B0LL40_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024153</name>
        <name evidence="3" type="ORF">PGT21_025644</name>
        <name evidence="4" type="ORF">PGTUg99_022724</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LL40"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF6">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="199"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1113804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="488" mass="55110" checksum="4622817392DE8C54" modified="2019-11-13" version="1">MLGDRLKVFVCISILLDILNSNIWTNAINPKLTVSSKHKSIKTENLLLQGKNQVYELKNGLNAAVKIPIEIDEEKNERNCGTVEIDAKPIALEATGIENREMSGENLIIKPNVSEFFSSSKNSVYKYDQEISKKKLTLLDHGFYKSTCFLYPNPHNEPDEYCILINTTINNGQGCVIVTPSKYLEGFFKNGLKISDDPSDLRTVDIVQMPEKGGMGAVAACKIQRGDLILQQSPVGLFPYLEKIWKTPFGHSIRRQAIDHLPLKTQEQISFLAGKGQNKDQFISNMIDVNLYDNYHYVSGTEISFGGIYLKVTRLNHSCKPNVFYYIDPMTQMVHLKAYETIEIGEELTVPYWSQELDQASRRSKLKEIYGFDCTCSHCMMSAELVRNSDQQIARLVQLSKLSQGSNLSESEIKEFLKISKEERIPKFRAMANLNAAKFYNSKGEIHKVRKYAEKAKLMGDLEGGSKWSPFDASDLAILLSENGNLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYQ5</accession>
    <name>A0A5B0LYQ5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein CPL1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYQ5"/>
    <dbReference type="OrthoDB" id="439917at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048661">
        <property type="entry name" value="CPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038955">
        <property type="entry name" value="PriA/CPL1_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35192:SF2">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35192">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21671">
        <property type="entry name" value="CPL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023080568" description="Protein CPL1-like domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Protein CPL1-like" evidence="2">
        <location>
            <begin position="201"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="267" mass="29417" checksum="8AE05E9EFD1B5F2C" modified="2019-11-13" version="1">MKLSYSLPTVLSLLVGYIFDPIVGGAYDSKLNSVDNHMVQLDDKISELQWVAQTNGHPEVHQLCESAKDSLQKAQSAWKNIASTYGDKPWLASDSTYKASVEEHWYSCGSKLESVYGHPNVLPESGGHPSYSKPVQACKETYDTCQSGFKHIWEWESPKPVASGAYYKRQTKARRDLKSTDLSQCPTGETACPISPHSTGIECIDVQSELTSCGGCVSKHEGRNCMEIPGAAGVGCHFGRCVVFSVHPSYFLGREGLPILRRRRPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1Z2</accession>
    <name>A0A5B0M1Z2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MULE transposase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1Z2"/>
    <dbReference type="OrthoDB" id="2507564at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MULE transposase" evidence="2">
        <location>
            <begin position="152"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="515" mass="56239" checksum="A4854FF605C69CF9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M287</accession>
    <name>A0A5B0M287_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011959</name>
        <name evidence="3" type="ORF">PGTUg99_032120</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M287"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="134" mass="14454" checksum="36EDA533FE10B920" modified="2019-11-13" version="1">MVLAGGRWPREAAKVLESYPRTPPASISLNFCATGQPPAKGVDFSRTYFTSPGPSIFTSPISTQLFCPRSFHAETSRTANNFTPVFSPSAGDPEVKEGRSPRGQMNHHSSGSVWPGPAPPTKHDPPNSRPLLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2A0</accession>
    <name>A0A5B0M2A0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005125</name>
        <name evidence="2" type="ORF">PGTUg99_013635</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000482">
        <property type="protein sequence ID" value="KAA1070962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2A0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="193" mass="20924" checksum="4BF9EB3672B97CB7" modified="2019-11-13" version="1">MKAKEWADNTGAGIKEGDNLPTLAEILEKKCPCYERMYGIFGGKANVSALAQHNSGLASNLYPDPRDKGDVVDGGDSPEVFFFGWEATQEDQATGLTTPANGLEDDELPPPLNFSGTAMDPSPILMTQRSMAQANQAHQVLPETSMSQMRRPFPNQASGEASPAPHRESNPRGKTSLALAFENLNIDKLSYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M425</accession>
    <name>A0A5B0M425_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M425"/>
    <dbReference type="OrthoDB" id="10270463at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022928086" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13996" checksum="A9C8E36BF9C02601" modified="2019-11-13" version="1">MKLIHILALTLVLTGISIAVDPVLCEGCGHRTGHLIGETEEECGQYNKKDPTSVACTYLRQKKIQQCSRTECGQVFHMNIPIKKNGGTPLLFRQCKHQWSQPASRPTATTRSTHMYNFMPGAPRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M621</accession>
    <name>A0A5B0M621_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_009233</name>
        <name evidence="7" type="ORF">PGTUg99_031360</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="7 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M621"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="6">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="162"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="162"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1067291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1071478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="204" mass="21875" checksum="AAD2DAC0DB065935" modified="2019-11-13" version="1">MFMSGGKPVTGPALEPAQHYIQKGGRLKSQLSQTSASANTYPSLARVTHRSTSIPTPVNIHPNCRQPHPSLTTDSSTVFSGGIESTGSLAKTLPRQAYLVSLFQDTNLAAIHAKRVTIQPKDIQLARCLRGGIESSGSRTPRCQPHPSLRTDSSAFFLGGFESSSSRTETSPRCTPCRQPCPSLTTDSSAVSAPKPSQLVWWAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB78</accession>
    <name>A0A5B0MB78_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UDP-galactose transporter homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HUT1_2</name>
        <name evidence="12" type="synonym">HUT1_1</name>
        <name evidence="12" type="ORF">PGT21_035784</name>
        <name evidence="11" type="ORF">PGTUg99_028254</name>
    </gene>
    <organism evidence="11 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="11 14">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1073114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB78"/>
    <dbReference type="OrthoDB" id="1601at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1073114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1084825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="382" mass="41717" checksum="E456A45B7770DC4F" modified="2019-11-13" version="1">MAFVRLVICVVGIYASFLSWALIQERLSTTPYEDPSGGKPKYFKSVIFLNTIQSGFSATAALIYLLLKKNSSQSILQVLGLTNPTTSKAEKTPKQPNSRLAPKDQNRLALLGRCLQCAILNSLGSPFGYASLKHIDYPTMILGKSCKLVPVMIMNIILYRRKFAIHKYIVVGMVTVGISLFMLFADHGSKASKGAQQSSLFGLTLLLINLLIDGATNSTQDEIFSRFTISGSQLMFIMNALATIITLAALQAPLPTAISSFFGTSNSSGNEFTVAIEFIKSYPKVLQDILLFSSAGAIGQLFIFETLSHFGSLTLVTITVTRKLFTMLLSVFFFDHKLSFGQWGGIAIVFGAIGLEAYVKMSGHGKANLIIDEKQKAKMKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBP5</accession>
    <name>A0A5B0MBP5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBP5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023057122" evidence="1">
        <location>
            <begin position="23"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="75" mass="8352" checksum="466B1FDE59D13C40" modified="2019-11-13" version="1">MNLRSFISLVVALFFTIDVANGEDSCPPLIGPGCCPGGAICIDKQNPRKVRVAEHIGSYRYPLYRCLDYENLECK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBT3</accession>
    <name>A0A5B0MBT3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBT3"/>
    <dbReference type="OrthoDB" id="2503849at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="275" mass="28660" checksum="725652B1FCD6F4FE" modified="2019-11-13" version="1">MSSNTTHPVNNQPTKDYPQPASKPVPTTERRASFLSTLFSMPSPPVGRHNSVSSNSTASSLSPSSSSCSSMATPQHEAGRGPLTGSPASSLASPIDSKFSPIDWSYANESPHSRQTSLSDSLGGFSLAGSFRWPGSATSTAPAAAAASNVQPISIPNSSSSALPQPNDAHHHPNPAPTVGMSAPTRRTSVGGPAFRRAFELPTYTSTYTNLQDNPPQQHDSPAQADANSRRGRSGSLFSSFGVGAGAGDASKRAPGPPRRKLSPMGERMIRDHPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MD06</accession>
    <name>A0A5B0MD06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MD06"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="183" mass="20829" checksum="3804B1505B746F94" modified="2019-11-13" version="1">MQSFEEVDPSLLLASQDPTFSVPSLNSIASKPPPNKKHTRRTKKQMALARGEDITNNEQTASQKSHKLSQRQRGSQKKGTQSTQKTITENDNNPQFILTDYKNICSYLEDDKKFSKLYGDTKTNVGPKLPYISLIAKKLVGQKHHFRIVGEEDVAYRQTSWKVKNFADEIISIILMLEEEEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEL8</accession>
    <name>A0A5B0MEL8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Multifunctional tryptophan biosynthesis protein</fullName>
            <ecNumber evidence="8">4.1.1.48</ecNumber>
            <ecNumber evidence="7">4.1.3.27</ecNumber>
            <ecNumber evidence="9">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TRP1_4</name>
        <name evidence="22" type="ORF">PGT21_034149</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="8"/>
    <dbReference type="EC" id="4.1.3.27" evidence="7"/>
    <dbReference type="EC" id="5.3.1.24" evidence="9"/>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEL8"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000031">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="16" id="KW-0456">Lyase</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="19">
        <location>
            <begin position="14"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="20">
        <location>
            <begin position="250"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="21">
        <location>
            <begin position="522"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00018819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAA1075355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="733" mass="79687" checksum="3B77C040E5EA4642" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MH29</accession>
    <name>A0A5B0MH29_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011650</name>
        <name evidence="2" type="ORF">PGTUg99_008863</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MH29"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1126721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="89" mass="9864" checksum="38FC48965F1B0827" modified="2019-11-13" version="1">MGQCINPLQQSNLNRLELHQPVPCTFTSIHESSPLLSVETFLIRPVSRAKCAQRGGKTHSDCYLRTSSAEIVFHLSIESASIAKHDCVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MI11</accession>
    <name>A0A5B0MI11_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_27</name>
        <name evidence="2" type="ORF">PGT21_000470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000155">
        <property type="protein sequence ID" value="KAA1075706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MI11"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="251"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="746"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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            <begin position="975"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1029"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1029" mass="111671" checksum="1904983453646056" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIY0</accession>
    <name>A0A5B0MIY0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032622</name>
        <name evidence="2" type="ORF">PGTUg99_033890</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIY0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="118" mass="12675" checksum="3A76C632F261C820" modified="2019-11-13" version="1">MVDKRSKQTRREETTDITIHTNLSAPSGNTNPPAPSGNTPSTSTPAAPSGNTPSALILMLHHGASFLKLAPSAGRFPEESVQWMRPERPRDALASQHHLDVSKSIQVTLAKEKASNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2024-05-29" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJI8</accession>
    <name>A0A5B0MJI8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006903</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJI8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="65" mass="7410" checksum="257870B4BCBEE35A" modified="2019-11-13" version="1">MNIQNDCLDRSKFLFLTFKLEARESNFEQPTSFLRPTPSNTSEPLINFQTTSQIASPDSETQPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQC9</accession>
    <name>A0A5B0MQC9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQC9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="127" mass="14408" checksum="1104F67361E0ED4D" modified="2019-11-13" version="1">MRLSGCGNPCTPFSVSEAFHKPFTALDYILAYLHRPGSASPFDYPLSNYCRELDLCPNVFVSDIRLDARNPPSQLCTSSSRPGIHFLSSSQPPLDHPPSYQPLDRSSSMNWPRCHPHEPDSIYIWWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTF1</accession>
    <name>A0A5B0MTF1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_009303</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTF1"/>
    <dbReference type="OrthoDB" id="2496395at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="361"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="821"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="821"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="836"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="877" mass="98151" checksum="0625B6AB2D9A4FA5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTF7</accession>
    <name>A0A5B0MTF7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTF7"/>
    <dbReference type="OrthoDB" id="2508473at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="112" mass="11886" checksum="9D330838131C2347" modified="2019-11-13" version="1">MATLIASFSSSLTGAGWSKSLQPCWFNVGLMERPTVGSTSVNHRLPMQTLAQPSMSVVTPSVQPKDGWANGNGWPPLAGPLSNRSPNLTVGSELRERSATSYVGSTSTDHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWB4</accession>
    <name>A0A5B0MWB4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWB4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="152" mass="16221" checksum="89B05A6168A8918D" modified="2019-11-13" version="1">MTRRVLGRFQVNPTHLHPECFVECTGGHSNEYGVHSHALHHPLPMKSNLEMGHLHPDIRADADIRLLFPAIQASASAPAPAGGYPSAAAGIRAGILGYPPSKGSATDRLEGDPSGRRGTSTSSTGRTPFRSTRYLYLAGWKDFLPAGPSPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYH2</accession>
    <name>A0A5B0MYH2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYH2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022911931" evidence="1">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="57" mass="6129" checksum="1C76C601BA69BF9E" modified="2019-11-13" version="1">MTLISYFFVVVAISLLAEQSYCSPHPKPHGSTGALTPELVNILPVFDLCTVGKLRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MZL6</accession>
    <name>A0A5B0MZL6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">mRNA cleavage and polyadenylation factor subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">CFT1_4</name>
        <name evidence="1" type="ORF">PGT21_032598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MZL6"/>
    <dbReference type="OrthoDB" id="6109at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1081269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="128" mass="14450" checksum="56B6488F92408FA6" modified="2019-11-13" version="1">MRPMRWDRPLCFEQDGRPLAVGFLDIYPYVTGMRIFNNFLLLGDSVKGIPLEAFKNPTNIESQGGSKLLCRYKFNPSSERLQVKNEAKVIGIFFASLDGSISSLVPSMDAVYKSLQLVQTRLPDTFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0U5</accession>
    <name>A0A5B0N0U5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0U5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="167" mass="18591" checksum="D1227549FD9F2FD1" modified="2019-11-13" version="1">MLSAAFLWKCQKTTDPLVPSRPNQSHFPPLFWTLPLSMDRPKSKAMPSPAIVIAFLFMYSNYTSTTLHDEMTTILELIITTSLTSLLRPSRLPPAYLTTPPSTCRSRYPSKNHWVRLSKNADVVATLLPCYPLVVAIPSSSLSPRRRYPLAVAIPLPSLSPPSSVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3F3</accession>
    <name>A0A5B0N3F3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3F3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="120" mass="13758" checksum="DDC762A547EC8EAF" modified="2019-11-13" version="1">MQSQTVVVPTDQFKHWKVPPKTPVTKSALSHKFQKASSIHHQSSETAGAQQHCFETTPAPVTWVVILHPEERPVLSPSTIFYPQHHPWKQTTYFLNKFCHVVPISHCGMDLHQHPFCLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5R6</accession>
    <name>A0A5B0N5R6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS13m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_015483</name>
        <name evidence="8" type="ORF">PGTUg99_018413</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5R6"/>
    <dbReference type="OrthoDB" id="525520at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.910.10:FF:000004">
        <property type="entry name" value="Small subunit ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1083249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1094264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="203" mass="22240" checksum="ED9FD69EB33A8748" modified="2019-11-13" version="1">MFILGVNLPEHKLVRIGLTRFVGIGHYTANLICARLQIHKALTVGELSEHQIGRLSALLSSPPTTPPTNHRVAELDGIPAPSTLDFDSGPSPQPKLLSLADDPLQRLLIESDLRREVRANIAHHRAIGSYKGKRHALGFPVNGQRTHTNAKTARKLNKVERRAYSTSPLHQNSSSTQSLLNQIISIDSLHGFSALRTQPSPCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N728</accession>
    <name>A0A5B0N728_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N728"/>
    <dbReference type="OrthoDB" id="10480034at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="516" mass="60008" checksum="E17D886A4E474739" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAH9</accession>
    <name>A0A5B0NAH9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GAD8_2</name>
        <name evidence="15" type="synonym">GAD8_1</name>
        <name evidence="16" type="synonym">GAD8_3</name>
        <name evidence="14" type="ORF">PGT21_035412</name>
        <name evidence="16" type="ORF">PGT21_037269</name>
        <name evidence="15" type="ORF">PGTUg99_016100</name>
    </gene>
    <organism evidence="14 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
            <strain evidence="15 18">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000273">
        <property type="protein sequence ID" value="KAA1114069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NAH9"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11651">
        <property type="entry name" value="YPK1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000048">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="220"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="477"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1084789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1114069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1120212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="551" mass="61283" checksum="6B1AAC64B3D4B239" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAI6</accession>
    <name>A0A5B0NAI6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NAI6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="191" mass="21536" checksum="95301D090D976CA7" modified="2019-11-13" version="1">MPFPSPPSSLSSVTSAAPAAQELASPQNHALRREESLQPSYFLVEPPSNYHYNNARATSPVLVERAVVRPSPEVEKEDVRRRVEDYCHEQVRERDLAVIMTPAEPEPCGRFGFHRESYGYYFPSACSFSDVPGSGNAESPYYVNTPPPGAKEELSAADRGPFYAEVVRERTVHTARRRVKAKFLEYFRILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBF2</accession>
    <name>A0A5B0NBF2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HHT1_1</name>
        <name evidence="13" type="synonym">HHT1_2</name>
        <name evidence="14" type="ORF">PGT21_002644</name>
        <name evidence="13" type="ORF">PGT21_003134</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NBF2"/>
    <dbReference type="OMA" id="DSNRCAI"/>
    <dbReference type="OrthoDB" id="2501816at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000010">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1064443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1085308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="136" mass="15281" checksum="A32190F3551DC666" modified="2019-11-13" version="1">MARTKQTARKSTGGKAPRKQLAAKAARKSAPATGGVKKPHRYKPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESAEAYLVSLFEDTNLAAIHAKRVTIQPKDIQLARRLRGERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NC38</accession>
    <name>A0A5B0NC38_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032677</name>
        <name evidence="3" type="ORF">PGTUg99_018425</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000411">
        <property type="protein sequence ID" value="KAA1086356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NC38"/>
    <dbReference type="OrthoDB" id="2511494at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41317">
        <property type="entry name" value="PD-(D_E)XK NUCLEASE FAMILY TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41317:SF1">
        <property type="entry name" value="PD-(D_E)XK NUCLEASE FAMILY TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12784">
        <property type="entry name" value="PDDEXK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="375" mass="43078" checksum="352C175CE7AED0E1" modified="2019-11-13" version="1">MDTGKYMSVITDSAFKRLFGDVKTLIFFLKSILPEVELRQVTTIKRVQSEPVTPASWSRSTSSKSTTRRSTVSASQHVVYDPTESVGLYSGMRLIRYDIVCVTDKDTIVNVEMQMVTERYSLNQMVYYSARLITEKRGWSGSQIPPNLFEQNVEKDLSLKLKKSRQERTSKKWNNQLQPVYVVAICDFSLKCLPGVPPELRPAWLCWAATFFQGSKCRVYPDATLPAFLPFDHIQHYCIVSLPNFGIPADACKTPLEQIVWLLCHLGLKGYTIPDWVAESEDLKHFIETIRLAALSDTDRDAYQEELTQLQAHENTRLLAIEEAKDEGREEGREEGREEGREEGRQQKQKLLTTLQNMNSTDREAFIERQIKEFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0ND52</accession>
    <name>A0A5B0ND52_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024954</name>
        <name evidence="2" type="ORF">PGTUg99_019921</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000275">
        <property type="protein sequence ID" value="KAA1113781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0ND52"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="77" mass="8425" checksum="3093F6E537671BBE" modified="2019-11-13" version="1">MILDLTQNSQSAVKLGCVDGKPSVGLDCRRTSPMRSVSRAMDPAEIRLSSNTSKSDINDHHQVKLGLRDTRADGWPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFF5</accession>
    <name>A0A5B0NFF5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFF5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1087466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="110" mass="12221" checksum="A0F1CB78D6495636" modified="2019-11-13" version="1">MTLVTSSVGRLAYTASDRMSHTVCCDTRSKGGPLPRVSQQTACDIPVTAVLHHGQLHVAPARSDIWGFHWGFRAQSLRLLPQSRTRIRQGDGYIRGDTQMTVVLVGRAWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJS0</accession>
    <name>A0A5B0NJS0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021913</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJS0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1089551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="99" mass="10873" checksum="C17FE4CBC41046B2" modified="2019-11-13" version="1">MSSAPGVFPSSANPSNCNHLSRSIQLQRFQADHICNLACKLDPLTKKKDQPLTNKHSRNALRTYSSPLDQNIYHPSLGVSCTNLAILCLAVEDTPCCFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKM8</accession>
    <name>A0A5B0NKM8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutathione Stransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GSTS2_2</name>
        <name evidence="3" type="ORF">PGT21_008641</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKM8"/>
    <dbReference type="OrthoDB" id="2496913at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03192">
        <property type="entry name" value="GST_C_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03039">
        <property type="entry name" value="GST_N_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000055">
        <property type="entry name" value="Glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000608">
        <property type="entry name" value="Glutathione S-Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF252">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="63"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="191" mass="21573" checksum="014CDC3368CBD5DD" modified="2019-11-13" version="1">MAFHYADIPFIDERLSSEEFAKIKETLPFGQVPILTVDEKLVIPHETAILKYVGRLAGLYPDDLQEVVKVDAAMCIVDDLSAAVVACWAPEHPGKEMVTKIWIEERIPKLFGYIDRYLANAGTTFSAGNSLTVADLRLYWAITYFKSGRLQKLPIDLIDNYPSIIQLYQAIQSHERLASFIKAESQLVDTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLK5</accession>
    <name>A0A5B0NLK5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_017064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLK5"/>
    <dbReference type="OrthoDB" id="10471221at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022933668" evidence="2">
        <location>
            <begin position="25"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="252" mass="27562" checksum="B02D3B65E1DEFAE6" modified="2019-11-13" version="1">MKLCIALTLAAGLFLAEAPALTSATCLAARDASSNIKTLRSGSLDRRSCFDFLKKKKDDDDDYEKKKIYIVKEDDDDEKHYKKKKHHHHYDDDDKDEDYHKGGKKYDYDGYYEKEGYYGEKGHYGEEGHYGDNDYGYHRRYARSASDYGKGEQGGQGGGGDGGKGRGGDGGDGGKGQGGKGGGGDNDLYYNGKYQSDSKGRYSGQHHSGDGGFGDGDQSGDLYYDGRFEFEGQGSYKGEFHSSDGKGGGYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNP8</accession>
    <name>A0A5B0NNP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004351</name>
        <name evidence="4" type="ORF">PGTUg99_036829</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNP8"/>
    <dbReference type="OrthoDB" id="10299876at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="204" mass="22173" checksum="990DC9D3B72834DE" modified="2019-11-13" version="1">MRLADPKDLKAVEPIDHDGYHPAGNSICHTPEGSPIESAPSYTLTHSPIAVAKKVEQSQGTGVAGQQERPHWISRGKAYESGSSSSTSGNLNGESKPEQNQESGYSGHLDLRPENNQARESEYSDSHVQEIELTVEDMPPYVQLIVAFATESLNLCEKVLDVTADMSPSKISRKAALISVPMCLIIFCAVHYLLSLKVGESRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPF2</accession>
    <name>A0A5B0NPF2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPF2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="224" mass="24998" checksum="8FFF7132C64A96EA" modified="2019-11-13" version="1">MFPLPDYNFTFLNLLFTVPVKESTIRLTLHISIFESSPNLPKMNALRFRHFKCLNSFPQTSRYPLPTPTYISSCIPYTTPPPYSPDTLTASLPVSHLRLTHTTSTQYSILYSPKKFLEPSLAPLDLPLYRLLSTRGPHFSPLASGSLSFRGILDNASFNQPHLTLNIIVDASSWDVLSSPIFPFTVMPEITGDLCPTHIDCKFRALPIGSNNVLSGRVGEIAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPS7</accession>
    <name>A0A5B0NPS7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008042</name>
        <name evidence="3" type="ORF">PGTUg99_035414</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPS7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="135" mass="14591" checksum="C0C20482909A90B2" modified="2019-11-13" version="1">MLLLLILADDLKHLLHSNLEDNLKHETLWKTAEVKKPLQKSANVMMGDSETSSMEHAPSHKGHGHGPEATPSAPCTPKTASRHHNHPCPSQCQVGPTRTNPSPGGPPGLGVILASRGGCHWDQPKTQAPQQCHFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NS97</accession>
    <name>A0A5B0NS97_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011355</name>
        <name evidence="1" type="ORF">PGTUg99_013040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NS97"/>
    <dbReference type="OrthoDB" id="2500854at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="427" mass="49308" checksum="B6E30AC228B70A29" modified="2019-11-13" version="1">MSDSEAMSDSGSDSRSKIDKVGWQLGHMSYQYGWALGQIFRTVANDKMVDELIERNELIDQLESSLLPSIKDHINSLLLALDLQNSGKQLTSNLDLISEITRKIDDTLMETTGAVESVSAPFKYQSTHTRNLGRCKEVRCGRLHTKVRNIIIKPLCSLFESGAEFMRFWELSRKDPESLEYRKKMSEWMEEAQRASDISYRLIDQTIEWSRKSDLVILQETWLEAEESINHTLEDLTELADPMIDFEHEQIAIDEPERTRTPREHSIDVARLTIPIIKLTRIFFNKIINTTTKKRLFTLDPGIDSKSLEQLIEGPSKIVRCFQDLALHLETYTEEDTLDGHRYSNPFVILHKLLDSTLLLLSFHLVPLPPPVEGHSLRQNHFKAWFLDFQSQYHRATDRMLDVLYDSDPLEFDSDLDSNSNSNSNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSS7</accession>
    <name>A0A5B0NSS7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">W2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_021102</name>
        <name evidence="9" type="ORF">PGTUg99_010460</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000383">
        <property type="protein sequence ID" value="KAA1091752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NSS7"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11561">
        <property type="entry name" value="W2_eIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000031">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="7">
        <location>
            <begin position="242"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1067900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1091752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="398" mass="44280" checksum="C00864F6F316AF20" modified="2019-11-13" version="1">MAVGIVNIGKDARGDMFHRYKMPVLQVKVEGKGNGIKTVIPNMSDIARALARPPTYTTKFFGCELGAQTTFDEKFDRYIINGAHQADRLRELLDVFIDKFVLCSSCKNPETELMIVGRDELIWRDCKACGHRGLVDNRHKLCTFILKNPPESAKKKAKRDKKAKAGEATAEGGSSEDGHNSQDELTKRIEGEAVDEKTAEATSTLKDDDWSADTSAGAVKARMKSLEAGIQNGLSLADEDDDETPGGPYGVFSEWILTNRDSVSDAEILVKAKELGIEKKHKTVQILAQNLFTENAVKEVSKHHALFTKMVNSEKHQKALLGGIEQLCGEAYPSLVPLGVPKIIMEFYQADILEEEVIKQWGTHVSKKYVDKDVSKKVRRACEPLLKWLDEAEDEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NST8</accession>
    <name>A0A5B0NST8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024028</name>
        <name evidence="3" type="ORF">PGTUg99_017282</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000376">
        <property type="protein sequence ID" value="KAA1092305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NST8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137555" description="Secreted protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12780" checksum="DC545317880DD53A" modified="2019-11-13" version="1">MYRSFLITCALLANFIILHPTSVEAIKRGPCEEVGGCDYYERMYKLDPGQAGRECGVQYHCWEMHDHTHTCRRTTESFSINTHCGHQINNRGRCRAPHQSLSPAVCPGANVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NV94</accession>
    <name>A0A5B0NV94_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011711</name>
        <name evidence="3" type="ORF">PGTUg99_016044</name>
        <name evidence="2" type="ORF">PGTUg99_030268</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000139">
        <property type="protein sequence ID" value="KAA1128777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NV94"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1092702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1128777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="69" mass="8012" checksum="533390FB9CB32B76" modified="2019-11-13" version="1">MSWVTRFNSQFGDCCPPISWAYLLGRYYIAVGTLYPCELLELDCSREVFSLLNFYRSQSHLVLKKLIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVF2</accession>
    <name>A0A5B0NVF2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVF2"/>
    <dbReference type="OrthoDB" id="2507149at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="116"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="379" mass="43671" checksum="DD27C5D4F82C9028" modified="2019-11-13" version="1">MYDHEAERRRYSESVRLAERLGHPVAGRRPTTQSQPRHMEESSPRLEPFFGGFVSQDQDEHDLDNHERSIPIAAHAEYFRLRRYAEKRHRISCQWGLIDNQALATYLLCQQKTLKWTQPAVDFASPLDFCTCASINIHHRRVDLVDLLHRRPSQLIAFCKCTPDPIRLMHHGYFACSVEKPRSAFSIRLVQYHHLLWQASAVSTSAFIQSISTFLDNRSDSPLLNRGSNWKRRQLRVPFTNCIDLYRRILNSQRKLLEDGLQLTKADKWASKCPRCFGPSQTEIKQDTEEPDVIVAMDGNFQQRHYAYASKDEPREDQYPHSFLPPSKISSDVLAIEETEASAVGINPPCSDQHKAANDTRDESTWDKCDDNGLFASTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NVH0</accession>
    <name>A0A5B0NVH0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NVH0"/>
    <dbReference type="OrthoDB" id="2508387at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="116" mass="12664" checksum="568030A78826BA13" modified="2019-11-13" version="1">MAQTHPTTPDAEPTSPKLEPTSPKLEPITLKFEPVSPTVEPMDHPLSAEQSGPPQQSIPVPTAEPAKRAVKKSPTKISPVHDLRRSRRIFDQVHGQGSMSLKNLKEHADVKGGRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NW41</accession>
    <name>A0A5B0NW41_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018175</name>
        <name evidence="2" type="ORF">PGTUg99_024950</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NW41"/>
    <dbReference type="OrthoDB" id="10556064at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="125" mass="13097" checksum="3EA27A4B05EE70B0" modified="2019-11-13" version="1">MASFATSTSPLIGSMSKDINPVRCSCASADLSLACSSACGNLLPQAESNGLKLLVPVTKDCTKPCNLAPSGPHAGNRSPLRLVRNRRACTASPSNARSAEEIRAQNEQSSGVTVEFFGCFLHQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWR4</accession>
    <name>A0A5B0NWR4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011089</name>
        <name evidence="3" type="ORF">PGTUg99_029134</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWR4"/>
    <dbReference type="OrthoDB" id="2500788at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="296" mass="32840" checksum="E6513812469357E2" modified="2019-11-13" version="1">MRCAVQRAAAPVESRQNSEQTSNAATATTEICEDGELESIQTAQINVGSQSDHITLSRKSSVIHRPRPLGRGRGQSFSMPYAEFDKLMTLKAAQTWSTNYRCNILDCFFSVIVEPQEPRVHEGPNARLCNRAQKHSSPHSIHGPRFSVASRRSFFSSLAKLDGNGLDLDLHLPSDSPLDNKVELLSPEIHAQGLDRKSKRSRSNKLKKNHGSFKLKKSSSTLQPKKSDNNNNDHPLKAAPEPFLNLPPKFPDQTHCGIFLPTTCFHKLKQICAANEAQPVIPMNCLPNPGGRCFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P1M7</accession>
    <name>A0A5B0P1M7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VPS37 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_029220</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P1M7"/>
    <dbReference type="OrthoDB" id="10260857at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000025">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678:SF2">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51314">
        <property type="entry name" value="VPS37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="8">
        <location>
            <begin position="108"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1094752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="192" mass="22659" checksum="CFF651C3CE422C05" modified="2019-11-13" version="1">MASSSTQQQETHNNKYQSTQPTIDDQLSREFPEIGAMSKEDLQDLLNDPDFFHAMLIRQPELKRPIEDHQVAINRNQALAERNLSLRPKLEELRDQVTTSFNQTQQTISQFNDLLKQQADLYQPYGESASRSRLLTALHESDKLTESLANQFVVQGLLEEDQFIKEFREERTRYHKRAWVANRWSEGYFRWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8L3</accession>
    <name>A0A5B0P8L3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_019878</name>
        <name evidence="2" type="ORF">PGT21_032521</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8L3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="97" mass="10728" checksum="994912902EA38313" modified="2019-11-13" version="1">MDCHLIVCLDCLPFRLITQPPLFNSVKRSKCNNSKPIASKPLGPESFMKTQSDGTDPEELDDFSTCNFYSKPNKQSSSGSIVEAKLVAPAVIVQYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8R3</accession>
    <name>A0A5B0P8R3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_007196</name>
        <name evidence="10" type="ORF">PGTUg99_031865</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8R3"/>
    <dbReference type="OrthoDB" id="77405at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF3">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="302"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="449"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="543"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="609"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="647"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1097446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1134192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="725" mass="77698" checksum="FA7CE4E12B5F0D20" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJ56</accession>
    <name>A0A5B0PJ56_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1100983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJ56"/>
    <dbReference type="OrthoDB" id="2509786at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1100983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="144" mass="15698" checksum="201590D2630F55A1" modified="2019-11-13" version="1">MLSNSLSNSTISFCFSPDTSISSFTSCGSHLAVLGELVQCEEEYKAALRVPISLQNALAPLLDMKTTAHPIIQRDWYQEAEIGLILYLAGTSSASSEEQSASLCQYSLALEASLYAEENRKLAGQTKLSPTSIIKSIKNWWISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PK47</accession>
    <name>A0A5B0PK47_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PK47"/>
    <dbReference type="OrthoDB" id="3230070at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09276">
        <property type="entry name" value="Rnase_HI_RT_non_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="56"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="277" mass="31440" checksum="1063004780D760FD" modified="2019-11-13" version="1">MLDQQSLTRLNISEIETIYQHQIQPWENFSLEVNNMKMKKENAREIVTHQIAQKKNNSENLLFTDGSNIPEQGTAAAALLNNSTSFACRVNNAEDSSSFEAEITAINIGLEMFKNNLNNLLSGNQHTIFDKQLNIFCDNQATLTTISKPPKSTSLQFRFNKIFTLLKFISSNSTTPIQLFWCPAHVGIPENERVDSLAKEATSDIQQFHLENQPQTLTNLQQYKFNKSKNPLLLLFDIPTLPYDSDNSEENTPRRVLGGYNVTCHPSLEEEKWVSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PKS2</accession>
    <name>A0A5B0PKS2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_030543</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PKS2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023044344" description="RING-type domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="72"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1101811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="208" mass="23051" checksum="2E0642FEC514E98B" modified="2019-11-13" version="1">MFINLIGFLIFQSVAQLTARTAVDLVGKLDKTNQPLDANAISASNSVISSSNMGPLPESNTLHCHSKRGLKCEKCGTSVKQSESASTWDCRHIYHSGCAEKLVKEGEKNCLICKAEFLGGFPPWYLPQNSNSGGSVYRSESTQQAPPHVQTWYFRPPSPGRYPQPSHVTPDYSQPQHDTPGYPDSYEYHPQALEAFGSITPQPRHHNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PL51</accession>
    <name>A0A5B0PL51_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012631</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PL51"/>
    <dbReference type="OrthoDB" id="2502001at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022861858" description="Thioredoxin domain-containing protein" evidence="2">
        <location>
            <begin position="32"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1101238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="42147" checksum="84FD44D66B9A0985" modified="2019-11-13" version="1">MMMTTSTRIATRSMFIKILISLILICSHVHAHQNEQIIIDFDGGDDHQQATTATPTTTLDTPQAVPTGIPAQLIKKVTGWNDTIVAKLLGQIPPRPPHPFVVPITDENYEEVIESEIDSVQPGWGSDQDTVWVISVVASDRPSTLFDDQFDKLASNSSLAIKPIPPKKREDEPDVIQKDPSDQYLFKPQIRFARIDYMANTDFTLTKWLIFKCPVMVVVTKRGDELRFFKTGGAPPSAEHLGEFLREERYLLKPIWNSTFSHGNSGEWVVVLMGRAFQSFHMLTNKVPGWLLMIVTSMLGTSLMQWLHKSPPKTTITRSTNTPSTRNSKNPPSENLASQELKKELAQAAVQLAQITDQKRGTSSAKTQSTTPQRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLB7</accession>
    <name>A0A5B0PLB7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLB7"/>
    <dbReference type="OrthoDB" id="10270224at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023025650" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13656" checksum="C7F010F68B6491C0" modified="2019-11-13" version="1">MKAFCSPFIILSLLSRSHADGGKEEFGGLEPAYPRSYVRTAIGFPFTINGNQQCKTCEIFVAQGCHSFKTPNDRPTNQPCDLAFVPIEHRDGESTCKTTKGAFHCYSGRDKTIGNGGCYDCASPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PM58</accession>
    <name>A0A5B0PM58_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028938</name>
        <name evidence="3" type="ORF">PGTUg99_010668</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000035">
        <property type="protein sequence ID" value="KAA1136242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PM58"/>
    <dbReference type="OrthoDB" id="10301892at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1136242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="260" mass="28081" checksum="BA931125F7D4DCA6" modified="2019-11-13" version="1">MKAQELDSHPALKPKEQAHLKSLLALIKADIENSDDEGDDERRAESDATQLANDLPPSSASHESTHRVTSIPAVQSLHGDAPISHPQLDLSLPARPNFDTPTPMHEDRQYGLFWLMAVPKVLARTPCPSVGEFSRVDKTPVVDNGFPNVLNPAYADIPDNSPGRKNSRGETSYLTEEGDWFLDSVMASVNQNQNLTPAQGNPVPPVPTPPADKQPPPAIPKRCGGESAPWPKRWPVCLGACAMLAALMRKARGADTTQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQ81</accession>
    <name>A0A5B0PQ81_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQ81"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="193" mass="20760" checksum="1530B89606D48F04" modified="2019-11-13" version="1">MVLRARRPPVEVTPPAWLSLVLNNMALMQISCTEVDTSCERSTQTEKNGVRTGLGVSATQTIKKIASSEELNRDQLTSLTGDWVEEPSHQDAICLNPDCIKGSGPTVGSASTECVQSTDRWVVTTHVCPTIDRQTPASVTVLTQRSGGPKESCITCGCLTVESTQPPDHWQMHDGLTEQSGNPLTSEVWPGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PR12</accession>
    <name>A0A5B0PR12_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">endopeptidase La</fullName>
            <ecNumber evidence="9">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_012028</name>
        <name evidence="14" type="ORF">PGTUg99_001180</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="9"/>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000143">
        <property type="protein sequence ID" value="KAA1128187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PR12"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000002">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000092">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000091">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon proteolytic" evidence="12">
        <location>
            <begin position="841"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="266"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="341"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="509"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="553"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="516"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="929"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1104147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1128187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1052" mass="117725" checksum="207424961E9B45D0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSX3</accession>
    <name>A0A5B0PSX3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000967</name>
        <name evidence="1" type="ORF">PGTUg99_002929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSX3"/>
    <dbReference type="OrthoDB" id="10277729at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="205" mass="23499" checksum="6FAC895E54328CC8" modified="2019-11-13" version="1">MGSTSFQETTPLTLSDLSSNQKLKLDRHWSLRPWLAHNIEDSVKEILLTISDPIFDNLERMMVWELNVYDCFKEANAFDSIQTKLVLNEWFPQQGDWELFFPTMDLLKSTSCFFSNDDTPITFVGFTLEDAKALLQLDYQPPASYGSSGSGQWDIRKKELAKHLVTMMDEMRKAQGYSSMILDAHFESLDLADEGTEASEPHLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT65</accession>
    <name>A0A5B0PT65_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024752</name>
        <name evidence="1" type="ORF">PGTUg99_022884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT65"/>
    <dbReference type="OrthoDB" id="2496355at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="482" mass="55248" checksum="6CCB9EEFBEA2EAF7" modified="2019-11-13" version="1">MDFNRLNISPPLSLSLCVDSQSSAQPSDRRDIAPSQTDLPGLAVLGQKKWNKQDYIHLLSGGYILEELLAKSSDSFHKRINSEECRTKMTAHLDYHLSLPCARLEVSRVTKKRKLAKVLHHDSASTQRSPRLFKLYNILIASLYHHHEDYLMKLKTPILNYRKQQEKLLEWLEIEIFTPGTGHPVLGVAPRPSLSWNSNDPQLIYGEIQLELLKYFSEKRTDPIDSTSLLLEKYLAEHTREYLALYQSSSATLEYPTQLSKNKSFRKILAYISNVAKDVILNDSPGDGNEKTGIRNNPLLSPYLEQFEEAFHESETETKQNVRSYYPGVPICICFEKNQPDIKPLRMVNVGKGSLLDIRIAGPRFRRLVVSLDRLHIRYLKMLKVEGRTLPRRGSLIQFLYDSIIEPKDSLPLVGSLEVRDGLAPWFSESYQGSKSFGELQLKIIKYFSEDLTDENMVDIPVFILAAWYHDKPPFKKVSTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT75</accession>
    <name>A0A5B0PT75_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014646</name>
        <name evidence="4" type="ORF">PGTUg99_016444</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000314">
        <property type="protein sequence ID" value="KAA1104985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT75"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137796" description="Hydrophobin" evidence="2">
        <location>
            <begin position="25"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="13678" checksum="EB66BA84FD2D09D8" modified="2019-11-13" version="1">MLSFNLMPIVLAILLQGQAIMVQGFECGASARPVGVCRIHRPPTPQPGPGSGGSTGPGGGPGEHHGVPKIYIDLKPAHHSNNNNFACTGPTTGPNRVHLYCCPRNKNTYDIKNLALTTFLYHYGCEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUC2</accession>
    <name>A0A5B0PUC2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUC2"/>
    <dbReference type="OrthoDB" id="2508271at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="266" mass="29813" checksum="AE1E376C501DC6CB" modified="2019-11-13" version="1">MNPTSFPLPSTGRGTRDETEYPAIPENFEQMPGEADRAMTEIPEDVEEYAKRMMRETMEEMLSEPGYMQLAKARSLHMSPEMMAAFQPNSTTSRDESNFTTQSQLPLPSEAFKRSMREQVEILTNDPHMVDLAKSTVLGLTPLSPLYTLTEGFRYPPSHPHLRVPNIIPVHQCNCSNCAGLQHQNHSPLPSFAPQGTAIHKPESRCLCTLHVDSNPGQSSRSNSHEPRNPDYMGIGNVEEATMVDSYPSWSGTPRELQHGWRCSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUE9</accession>
    <name>A0A5B0PUE9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026807</name>
        <name evidence="1" type="ORF">PGTUg99_011347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUE9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="76" mass="8431" checksum="E8FCE699CADAC91C" modified="2019-11-13" version="1">MNPLDKQLRGLLGLCMPDEFGKLEVEGCRTRRSQSVRTLAGLACLTSRLTMCAMRECLACQEAPVEESVVTWMPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUN2</accession>
    <name>A0A5B0PUN2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUN2"/>
    <dbReference type="OrthoDB" id="2503229at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="343" mass="38570" checksum="8F3F7576F46D6ADD" modified="2019-11-13" version="1">MTNDPSDGNINTGYATDQSQTRRRRSSRASSLVVVPNMVAPSSDSRVKVTRPATHKRPTVAETSSESGSVKEVQSESDQDSVQTDAIDPKGKPNTKKRKQRQEKPSSSKKKKTAPPPQRKGKSKKGKSLESGKEPAYNYDQDTDEGSITIQSREPPKAKKKDKYPIEDFFYPPFWKEGDKEGLLLNYKCCWCEVVYRKGEHTNGNLVSHRDGFTQGGKSDRGCRGRAKAIAAGVDLPLSVAEQRKLANLTSNSHKQPAISQFLQAQPLFVNRVLNQIIMIWQIRQALAWSRIEDPYLRAAFRYANSKATLYSRRWSATEAKKMYSMLKSEVFKELNVSCDLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PWY4</accession>
    <name>A0A5B0PWY4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PWY4"/>
    <dbReference type="OrthoDB" id="2497224at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="255" mass="29620" checksum="2ECA9E9F0B2DB5EC" modified="2019-11-13" version="1">MGPIHVPNHENASITMELSASQQETFLQVFNELGRHLCLYYIRLPISEAKNDVEIASTLRSNVQNLGQCIDEAIEQFSRIDTRAYKSWWHAMANEMEETLRQPVQTLHQEASGPEAEKVQHLCRESIPIVKLTRLLLNKMSRPTNSEVHPISLMEPRNLIALTHSTRMLAIDLHDFVESIHIFDPHPDLFNQEPVENIEEAVEETLQIINGFLDEYSDPNDENFSNRWKEWYERWRDAFDLAVERFQATLVAINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2C7</accession>
    <name>A0A5B0Q2C7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013452</name>
        <name evidence="3" type="ORF">PGTUg99_034218</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2C7"/>
    <dbReference type="OrthoDB" id="10054765at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="53153" checksum="03E0C4F71B67C366" modified="2019-11-13" version="1">MMTSKLDRLVRTIAAPPSLNHLWERARKTRIFARSTRHASSHIQSGRHGRSSPFRSSSSTSSRGYVRDAAAGPTISEQRLVDYFRLLARVQKSQERSLLTSIGTLSDQADRLDGIPLDALLEYEADPAGSRRVLRTDDDRGLESLGRNDGEVPTASPSITSTDSQEGQQELEGRRLIGTGILSLSYIIAPIDRAVSETHRSVDRDTPSQEHEQQHVVTCTGFLVQSQASNERILVTCAHTLWQSRALLQQSHHKQSQLEEDSRDRNTACNICSGCVARDQLGNVFLVKHVLGALASADLLFFKLLLLPSPSSASSLSLQQEDLVEQNEVSLIDSFRHFSSLNDHHNTNTLKKFRTLPVSPYPLPLKSPLSIPQLSPSSSSSSHRLGAVGFQWEVGRVVEYKDTMGIKATPGTYDPLNTIIFDVVPRNGSSGAPIVDHQNGTVVGMVRGFQSAYDLNRPLGFGTVSERFWELFLLPGFHSPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q310</accession>
    <name>A0A5B0Q310_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q310"/>
    <dbReference type="OrthoDB" id="2508371at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="389" mass="43654" checksum="07E4DE587D380BAF" modified="2019-11-13" version="1">MHTTNPVKSIPKIVNVEFPSGTAANTPQEIWPLSNVPGEMLDPQLQTTRRTSNIDTIAAVDQNHEQLFNQVSFIADELLRSPVRDEGPEQDIRISERSLGEFRLESSTRPAKRLKSGRDPQHCASTGPAVRAMYDGFAECLNEPGKIQYRVGPGQARSGFLGNLPVPRRGSQALQQSTPENQSSKSIVEVRAQEGMQGNLSNDHRPPDSSPLESQNPVEILKESHFKKRPIPLFSFPTLESCSSKQALSHPSNDFKQLPPTIQPNPAAVGHPSLLAFGYYSHQALAWFPGQVSQIASLGAHALAYQLLQHIADLDTKEKLQKMHESNGILIPFIYNLILKELSNTHWVRIKEVWGYLWQHWSQLKNVDSDEEVLRTEKRLFPTTIFRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA46</accession>
    <name>A0A5B0QA46_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_010279</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP186/NUP192/NUP205 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2019644at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11894">
        <property type="entry name" value="Nup192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1110098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="2170" mass="242001" checksum="8F3BF20C0937A510" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA70</accession>
    <name>A0A5B0QA70_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QA70"/>
    <dbReference type="OrthoDB" id="5353557at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="190" mass="20942" checksum="86736A75F94270E7" modified="2019-11-13" version="1">MDQSLISHLVDLVDKLRIALADHLGNRLISTRVYKNVITPSGTGRKMLGALLTKQFILIVPKGVDLIVCCPVLPSKALLTDQPLVIISLEDVLKALLGEPIYDKTDLDGHGHMAVPPYVPPEATCALKKETSFLLPSPDYDAKSSLYLSVGYEKRLRLSCLTGSHPKKLPHHSRSLTSRARKSIELCRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAK6</accession>
    <name>A0A5B0QAK6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAK6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="134" mass="14928" checksum="B2A1156318E0BA44" modified="2019-11-13" version="1">MFSHSSPRIYFHSSVPFLLRVARLFASAPNILYGAPGALSEKPAGRPRSSTINPEEKDRFFLSSINDNRYHPGIDRCRLPTRKPNLCACMLECLSGILRGGAISQPGILGEQIDVPYDLLGMAFPTLYKLYLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB40</accession>
    <name>A0A5B0QB40_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QB40"/>
    <dbReference type="OrthoDB" id="2502602at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="559" mass="61123" checksum="6D6D8FCD218FE345" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBD5</accession>
    <name>A0A5B0QBD5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBD5"/>
    <dbReference type="OrthoDB" id="2506775at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="296" mass="32978" checksum="B3AED7A9DE36D3C4" modified="2019-11-13" version="1">MSSQPTQSSMSNQSGNVSDATEVQTRRSSRVTTPMKRSGMIQPSPDSCRSITQPLLSERPDIDPSSQTKKRTEAPTNSDSTPQITPVQSQKPRNKKPTKKPKKTTGATSVGNSEANPGTTIDLAQDSDEENVKAKFKRQRKNLEEDDIKVFFSEPFCRKDDPADKPPSTYSCLWCKKEVRVSASSLSNLRVHRDGSRQTGRVSNGCPNRAKAIAAGAKLPQTALDEEKKKKGTQEITSHFARVEKFDNIILNRVVVLWLLRQAIPWNRVEDPYLQAAFNYCEPAANLFKRKWAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBE0</accession>
    <name>A0A5B0QBE0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBE0"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12159" checksum="777BEDBACEB1974C" modified="2019-11-13" version="1">MSGEQDNWFKDRPEFKDQGEKAQATPCPDELGSGQYEPMWTNFTPLWRCTLDYIWVLCKGINSISDIEVRALLPTHRTETLEPGLPRLGVEPSDHIALMAEVIIPCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCA5</accession>
    <name>A0A5B0QCA5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_033009</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCA5"/>
    <dbReference type="OrthoDB" id="19679at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019148">
        <property type="entry name" value="Nuclear_protein_DGCR14_ESS-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940">
        <property type="entry name" value="ES-2 PROTEIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940:SF0">
        <property type="entry name" value="SPLICING FACTOR ESS-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09751">
        <property type="entry name" value="Es2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="131"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="361"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="486"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="567"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
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            <begin position="413"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="499"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="618"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1110858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="657" mass="71884" checksum="EA757712A8CFCA24" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QD33</accession>
    <name>A0A5B0QD33_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036831</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QD33"/>
    <dbReference type="OrthoDB" id="10382089at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="304" mass="33322" checksum="2E5F98971B9D8AB3" modified="2019-11-13" version="1">MPRFNKHTPGPRASNLYPIHSRMNCPVPSSPLERGQVLGSPIQLDSRELSRPPILNASPIVPPSARHLASPLSLILKGLPCLHWLIRRQSRGRFLPKPPKPLELIGCLQGWWGSILKIEATSEWCRPASNSRGSLGSPVACCSFEVAELLLNHLLLLLSSNRTVIEEPWTLPQAPRPQNLSTPSNVRSAVHIAPPTLTSASQEESAVSDEDDLESSSSQHTASSNQSYPFVCQHCVASPDPPGFYDVDKFHLLRDHQIDSLLSRSADPARFDRPIGSATSSSSTVVICHLGVTFVTWSKVFFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QEC0</accession>
    <name>A0A5B0QEC0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QEC0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022700450" evidence="1">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="67" mass="7203" checksum="13CBFFCB7E6C3B3D" modified="2019-11-13" version="1">MPKNSIRTALSSFLILILLGSSTSQPVSNLTPRSDVLSTGPPHLIFSLGLGEPFLSEIGYKINSIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFR6</accession>
    <name>A0A5B0QFR6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1112056.1"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
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        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="635" mass="70822" checksum="70748BE0C7B71E0C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHB5</accession>
    <name>A0A5B0QHB5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001632</name>
        <name evidence="3" type="ORF">PGTUg99_012670</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000045">
        <property type="protein sequence ID" value="KAA1134807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHB5"/>
    <dbReference type="OrthoDB" id="10289471at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="111" mass="12096" checksum="C59E50F0B06798B4" modified="2019-11-13" version="1">MGIGCCPAADSTQIPGRGYASGPPGMGAHRGPTNIPSKLRRNAPSSQTCHPLRFAYLYGCVQLAGFTKSARAHACWVYHGKHFYNVDAVLYDRPLCFFTHFSSVSFLALDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIF8</accession>
    <name>A0A5B0QIF8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_012653</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIF8"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="3">
        <location>
            <begin position="256"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1112849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="471" mass="50802" checksum="C6632FDE7AA2C223" modified="2019-11-13" version="1">MDEFIAGMEGADKMTYIDFARKVGAANFFCGIGLSCNAGQLCNPVAGKNWLVLVAIQHWNTYLNVLYQGVANAVTIVRDASAEMITDFIPDTVMDKSIYGWAIATLVIGVLGGFSGALTPLLITKPVAQAAGEYGTATSIGSAAASNFQKTQEMQIAGDVVGVYRARSAHDAAIVSKAFEEAAKKAKPLPPMKQTTSSKSLAISSAPKTTAADHKKRSIMDENHANSVNKSPILYNIRPSTYPSQPKTLQKRGGPPPSAYAYQRWAEIDTHLAALQNRLQKDIAAVSHAGLTEPIYNNNGLAKNLLGGSMFEKFPTQIDEEEKNKAFAKITALNEIFKAQNMFITLGCDGCGDVGPGGAWPQDKFLSYCTPDGSMRNIIRAEGIHARNEVRNAQLLQSKYGFTTEYLTTRAINCQNKYGFYTLGKAPEPTNVDSDCAFSIPVCDCSIHVVHKKRKKGKGTVRACRAAGVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ89</accession>
    <name>A0A5B0QJ89_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1279 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ89"/>
    <dbReference type="OrthoDB" id="5580261at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018811">
        <property type="entry name" value="MRX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002:SF1">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10306">
        <property type="entry name" value="FLILHELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="27049" checksum="0664F3FD24F7364E" modified="2019-11-13" version="1">MIGRRSLQVWRTRTRAQTQGLSRLARDGPAKRRWTSSSSSSSSSSTTRGGESRMEKMIRTIQARYPHISPTHLPSLTAAFVLLHELTALVPLLAIFGVVQTTGTGAKLVHFIRHSLPPPSSGSSCDQEPASSSSDQKPASSVPQDRSDPTRHSAIAGSLTQALDESAAKVSKLAERYGYSSGQEAPEDDGACDEESKSMLENGVLASGLVAYLCVKILLPIRVSVSVYLTPSLARQIRRMAHWEPKRPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJI2</accession>
    <name>A0A5B0QJI2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_027261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJI2"/>
    <dbReference type="OrthoDB" id="2499804at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="11328" checksum="CF4D75815D91059B" modified="2019-11-13" version="1">MDQLRNGQSIRLTRSAVARGRSWYLDAVNGGPTSMDILVTWLSTPGNYDRWLSTPIPGPHREDFCTEIVEIMQQHGIHHRNAMGKSSVLIFLAQALTPIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QT46</accession>
    <name>A0A5B0QT46_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_009590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QT46"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="106" mass="12893" checksum="3E394AB8686A9921" modified="2019-11-13" version="1">MEKFGVGLDAHEVVEFRRNFEFMKAVGQMDVWKTLFMDYGWSLLINTTRRREVPQKIWEERRAFYWAKLKESVKFYDSLINREVEKDPAWDGSIARCRPFYLTKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTG6</accession>
    <name>A0A5B0QTG6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTG6"/>
    <dbReference type="OrthoDB" id="10398931at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="104" mass="11393" checksum="9A65BDAD93A7B148" modified="2019-11-13" version="1">MKRTKKRPRQRSPSPSPTPSGRSDARPQSTQSKAADSDIVVNDGSNTTSGLQSRASQARQANNSLDPTDHDELLSAAYANYNTPELSTQLDKKGRRMIAYPCKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QUB4</accession>
    <name>A0A5B0QUB4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031203</name>
        <name evidence="2" type="ORF">PGTUg99_020706</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QUB4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="121" mass="13884" checksum="85B398C393F9D30E" modified="2019-11-13" version="1">MCIRKLDSSGVNKGLPHLVLGNLEVDANRRDKLDAGMRLLIFLVEDSDPHDQNYFYRYFDTQKEVKQWSRGAQWVSYCKEPYSLRTNNSLPRSGGLMRVSFSVTRNGFLQSEPLQGHLCDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVQ9</accession>
    <name>A0A5B0QVQ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026713</name>
        <name evidence="3" type="ORF">PGTUg99_034090</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVQ9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="105" mass="11892" checksum="07D9638FAFAE64E4" modified="2019-11-13" version="1">MPGLGDVNERTIHDVSVGKFRSPSEIRGFANAKRLRFAHTRAPKGSKVGATYVPFARKHQPQKFFIRMNIYPIKPCARTCCPARASDKRTGSSRGRRVDQDHHKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXR9</accession>
    <name>A0A5B0QXR9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031672</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXR9"/>
    <dbReference type="OrthoDB" id="3365211at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021369">
        <property type="entry name" value="DUF2985"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35872">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G07110)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35872:SF2">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G07110)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11204">
        <property type="entry name" value="DUF2985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="407"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="565"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="590" mass="66279" checksum="D3C0A8717E8B1652" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZY5</accession>
    <name>A0A5B0QZY5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZY5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022788360" evidence="1">
        <location>
            <begin position="29"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="8914" checksum="3868338B40209868" modified="2019-11-13" version="1">MFPMTNWSLYLTKVLLILCFLRNQVAYAKQNSNNKGDYYCELEPKKDVQFCCKPGVIEGFPPTAYPNFVDAGCTHITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0I4</accession>
    <name>A0A5B0R0I4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_013817</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0I4"/>
    <dbReference type="OrthoDB" id="2503718at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052758">
        <property type="entry name" value="SRC_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44200">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44200:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="343"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="472"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="588"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="608" mass="67799" checksum="FC577B27C5D480B6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3R8</accession>
    <name>A0A5B0R3R8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_034598</name>
        <name evidence="7" type="ORF">PGT21_037195</name>
        <name evidence="4" type="ORF">PGTUg99_018415</name>
        <name evidence="6" type="ORF">PGTUg99_023483</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="4 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000475">
        <property type="protein sequence ID" value="KAA1073007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000273">
        <property type="protein sequence ID" value="KAA1114130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3R8"/>
    <dbReference type="OrthoDB" id="660550at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000169">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF74">
        <property type="entry name" value="AGR407CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033845277" description="Peptidase A1 domain-containing protein" evidence="2">
        <location>
            <begin position="36"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="3">
        <location>
            <begin position="57"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1073007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1084712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1114130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1120138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="392" mass="43114" checksum="800085F284219F14" modified="2019-11-13" version="1">MFTSTRSANATHRTGCSSSTLYFLVLSLSFQACLGGSTSEKKKIKTSIPIVNEKHLYTIPVGFGSPPEYHNLGIATGSANTYTGSVLEYKKTESTYDLHQQLFIPYGFGNATGKLVTDTITLGESETSVKLTNTTVGNVTKLVGFEGFDGLLGLGLSSESYPRNEENIGQLLTPTGLMYDRGLLEKVMFGISFMPTRKDRDPNGILTIGGVEPSRFVGDLTWYPCSSYRTWDWSASISYGENELSGKSIMGAFDTSYTNTPALPRDLFDKYVLSIPGALWDDSWLSLNPSGSVNKYMLKIPSSSVAEMEDLCFTAKDVRWCLCPEAQLVPDGLITDGEPGYRYGYVSPLQTTVNQEAKFILGMKAMERFYVAFDLANYRVGLAQTEWTSSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4T2</accession>
    <name>A0A5B0R4T2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4T2"/>
    <dbReference type="OrthoDB" id="2337740at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1120015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="109" mass="12992" checksum="A8E4FEDC429DE329" modified="2019-11-13" version="1">MDTLRVEWDWQHNHDPFSANEIKQNRIPKMVKQWLTERVISGLNWKSIHQLMQTTEIIQMDSDAVTPEANIIKYDHVKNLIRTRMGVLAKQNANAFESIQLWEDKLRQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R6T2</accession>
    <name>A0A5B0R6T2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mitochondrial 2-enoyl thioester reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ETR1_3</name>
        <name evidence="7" type="ORF">PGT21_035634</name>
        <name evidence="8" type="ORF">PGTUg99_016859</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R6T2"/>
    <dbReference type="OrthoDB" id="7482721at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08290">
        <property type="entry name" value="ETR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000112">
        <property type="entry name" value="Enoyl-[acyl-carrier-protein] reductase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051034">
        <property type="entry name" value="Mito_Enoyl-ACP_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981:SF2">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1095112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1121376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="220" mass="24879" checksum="403A5B10AEFB5E4F" modified="2019-11-13" version="1">MSVNMSTAYRMIKDYLPQPSSSSDTWIIQNGANSSVGQYVLQLCKAWNLGCIGLIRDRPNVEELKAYLTRLGSEDKTTILTYDELADRPKPSQSISLGLNCVSGNAETVGMMKWMSNGSRLITYGGMSMKPLVVPTSLLIFRDLKLEGFMLTNWRMKASKSEYREMLEDLVRLIDRGHLLEHEHATIVDLNSDLAEKTVRETVKQSMSGRAGRKFLFRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RET4</accession>
    <name>A0A5B0RET4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal protein L37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RPL37A_1</name>
        <name evidence="11" type="synonym">RPL37A_2</name>
        <name evidence="11" type="ORF">PGT21_026170</name>
        <name evidence="13" type="ORF">PGTUg99_013546</name>
        <name evidence="12" type="ORF">PGTUg99_014105</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000339">
        <property type="protein sequence ID" value="KAA1100676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1123969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RET4"/>
    <dbReference type="OMA" id="RMAYLKH"/>
    <dbReference type="OrthoDB" id="10259236at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000001">
        <property type="entry name" value="Ribosomal protein L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_eL37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_eL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_eL37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003214">
        <property type="entry name" value="PRK04179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9 13" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1079869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1100676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1123969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="94" mass="10662" checksum="49B8C10D0CE68331" modified="2019-11-13" version="1">MTKGTSSFGKRHSKTHTQCRRCGRRSFHKQHKTCAACGFPAAKTRSYEWGAKAKRRHTTGTGRMRHLKDVPRRFKNGFREGTVATRKTASTPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RKE0</accession>
    <name>A0A5B0RKE0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021182</name>
        <name evidence="2" type="ORF">PGTUg99_050023</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000178">
        <property type="protein sequence ID" value="KAA1125413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RKE0"/>
    <dbReference type="OrthoDB" id="2512050at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1125413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="295" mass="35177" checksum="A5B4AAA7DEE77696" modified="2019-11-13" version="1">MYKQEMISEKSYKKFFQMKNTMELAAINLVATFHIHSAAFPLANQNLEVMFERWYCSNYKTLSEVLEDRERRYFLYLSLQVFSKYYYNDGMYKTKLHKSFFKDDKTFHTLEKYHILKNSISQEEQNLLKQTNSGYSHAKSVVKELIEDFEKEETQSRNLAIKGNRVKSFSFLQFIEENYGLDILDIETTTLFKEKFDLMSSSFQFISEIKNLTDYFHYKFNENFDRMPHHPVSTSLSPDQEILMIYKYFQVVCSKHSALLESIDLQGYSHLLYVNSLKVDLEKDILNVISQHNFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RKG1</accession>
    <name>A0A5B0RKG1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013065</name>
        <name evidence="3" type="ORF">PGTUg99_009968</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000182">
        <property type="protein sequence ID" value="KAA1125174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RKG1"/>
    <dbReference type="OrthoDB" id="3250324at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="13286" checksum="58445DF5026C196A" modified="2019-11-13" version="1">MPRGKQSRSSQITSSSTKGTNTTGPPNNQPTVSSSGDSDSDSDSEANIETCKGLSQDPPPTAPTPTPSNKSTKNTRNLASNQDSEASTEPASKKRKTTSDVWAHFKEQGLGENLKAICCSCKTPLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RY81</accession>
    <name>A0A5B0RY81_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018766</name>
        <name evidence="3" type="ORF">PGTUg99_017848</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000110">
        <property type="protein sequence ID" value="KAA1130338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RY81"/>
    <dbReference type="OrthoDB" id="2509336at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138217" description="C2H2-type domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15804" checksum="1A19CFE1CBFDF4EE" modified="2019-11-13" version="1">MMKLFINPMNFVALACLSNIQLVLSSFASRNGALPDCYLKSRGCLGTGQPLSEAQVAEYNFPAYDWCGEKYVKWPNCSIWREKSYYECDACFRLFRQNKGPSMDQRASTCRHYNKEPVERPTLPLLDQSSLATGPSIAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S077</accession>
    <name>A0A5B0S077_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Kinesin-protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">KIF11_1</name>
        <name evidence="12" type="synonym">KIF11_2</name>
        <name evidence="12" type="ORF">PGT21_011285</name>
        <name evidence="13" type="ORF">PGTUg99_024235</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000073">
        <property type="term" value="P:initial mitotic spindle pole body separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF12">
        <property type="entry name" value="KINESIN FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="115"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="152"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1133"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="883"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="8"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1177"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1226"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1288"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1314"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="243"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1080553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1131521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1323" mass="148700" checksum="6706EBD1836D5CF7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SBN0</accession>
    <name>A0A5B0SBN0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SigF-like NTF2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032072</name>
        <name evidence="3" type="ORF">PGTUg99_030859</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000070">
        <property type="protein sequence ID" value="KAA1133894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SBN0"/>
    <dbReference type="OrthoDB" id="2344312at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057514">
        <property type="entry name" value="NTF2_SigF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35393">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35393:SF1">
        <property type="entry name" value="SNOAL-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24840">
        <property type="entry name" value="NTF2_SigF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SigF-like NTF2-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="187" mass="21368" checksum="515B8AE4118CC13F" modified="2019-11-13" version="1">MENPRAEIADVVRSITEPQDPETVLKNIEKYFTEDAFILHPLANQPELARGRDNLKALYYVFRKATKDNKIHFHAVMFSEDLMHCTLDLTEDVKDMKSILFPSLSPHPISVRFLVRVDLRLESDGKYRIWRQHDNFISDLGMSGFKLFPGAGYISDFLKSSGALFTIALGRYFAGNLITQQKESPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SIP2</accession>
    <name>A0A5B0SIP2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_026121</name>
        <name evidence="9" type="ORF">PGTUg99_017043</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000006">
        <property type="protein sequence ID" value="KAA1137640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SIP2"/>
    <dbReference type="OrthoDB" id="4540492at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1089654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1137640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="218" mass="23486" checksum="CD2FE116F2BEF98F" modified="2019-11-13" version="1">MSDNQFGFLTASYTIGGFLSSVYASKLAGSRGEQNNLLLSAISIGIGSLLTSLSNFFTMILIGRTIVGIGCGINTFLVLIYLSEISPSQTRGSVGVMNQLAIVFVIFGSQLVSLPLAKLFPWRWIFIISSAFSTLQVITSPLVLNQKDQSSLHSDHLADLLDLESNSIPYPVADSLHHIQQTGITSGSSTLFHRYLLLSLCYWPPSRLNGLGVVGIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SJ58</accession>
    <name>A0A5B0SJ58_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003210</name>
        <name evidence="3" type="ORF">PGTUg99_007243</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000011">
        <property type="protein sequence ID" value="KAA1137163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SJ58"/>
    <dbReference type="OrthoDB" id="2499418at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1137163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="100" mass="11245" checksum="77C62ABE0FF6382B" modified="2019-11-13" version="1">MLQLAQFAFIDPSLAAEVNRMPEDSRTLHLRSTLNYSSITPPDQPMYDITVDVRGRRLPRVGFSFSHWYPSGLGRWISAVVMASGTVFWFLGACVLTPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LIF3</accession>
    <name>A0A5B0LIF3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014731</name>
        <name evidence="2" type="ORF">PGTUg99_005817</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000515">
        <property type="protein sequence ID" value="KAA1064121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LIF3"/>
    <dbReference type="OrthoDB" id="2503691at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="406" mass="42536" checksum="46C6CF5AB210DD21" modified="2019-11-13" version="1">MSLCYSSSFEELSILVNCVATISAMLDWTLDPNPSSFLSPPGTADQPNSSAHTPATTTTNTNTTHDSSNVPIILRYALQSLATLKPPNIDDLIHEAAVEFHLDPRSIYLAISINQCPVKISLSLYRRIAPLSTIYVQHHTPAPIFNTLQPGGPSEPLSGPDTSTSTSTNTTSAPSTTAATSNNGQTNNTRPPAEQAIHDFLNSFATASTVQNPPQPAPINDASSAVQNPLNGSSPPTIASLLSSSIPPNGLDSSQPSSSTAPGGPNAITAAATGAFTNPTNPQNMPTGNNRTSKKRFLINSLASANTLNQHPPSQHQPQQRGVSQGHDQEPSQSRRSKFPFLANRRPSAGSPSSSSTLNPSSSAISIADSPSDHSPRFSANGPPSSHDPSNDIFSMDLFNSLSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LIX0</accession>
    <name>A0A5B0LIX0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029087</name>
        <name evidence="2" type="ORF">PGTUg99_004721</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000520">
        <property type="protein sequence ID" value="KAA1063833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LIX0"/>
    <dbReference type="OrthoDB" id="10271264at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036136973" evidence="1">
        <location>
            <begin position="24"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1063833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="101" mass="11060" checksum="3A428CADD9E0B061" modified="2019-11-13" version="1">MNLSTSAILGLLVMVAATGLVDAEGKFFCSKDRPEAFCTTPHPNGNGEEYDMKYLNSQPGQANVCGQFTQNRCCNQGTWDKAQTRNNVLQRATISHECTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJP0</accession>
    <name>A0A5B0LJP0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_000430</name>
        <name evidence="4" type="ORF">PGT21_032694</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000206">
        <property type="protein sequence ID" value="KAA1063848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJP0"/>
    <dbReference type="OrthoDB" id="2495958at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366015" evidence="2">
        <location>
            <begin position="27"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1063848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="659" mass="75671" checksum="5EDF813A061882F1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LK76</accession>
    <name>A0A5B0LK76_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LK76"/>
    <dbReference type="OrthoDB" id="2499340at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="209" mass="23470" checksum="EFE902A84B8E9DC6" modified="2019-11-13" version="1">MAPRKKKTPAANDQPPASLELLKESYQALQEFHQTVTEKAQGVSPNRFAGANNLSIFQQLLEYFKTIIDHQTGNQKDDARLVKECSRLCDFFRHQVASLYALPLKNIFAPIKPAFPVTSSPILGLMSYIYEAKLERCRAPFSLVRETRFKDDSEHIGQCWERILAAFGEGIRVKLTFNPNDKLLDAGEALSGGSAENKELIRDPTIFGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LKK5</accession>
    <name>A0A5B0LKK5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_014788</name>
        <name evidence="2" type="ORF">PGTUg99_029783</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000506">
        <property type="protein sequence ID" value="KAA1068247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LKK5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1064785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="131" mass="15068" checksum="FF92B1FEBF006B09" modified="2019-11-13" version="1">MYTTHLNYSSWPSHSLDLQFNYTQSQKPPYPTQTRLPWPKLHPTALATDETLVTNNPHTRLIHQITTHPRLQFLHNQLPAGLFDPSSNNTLTSAHQQLSTYKLISPTLIPYLKRIQSISLCRTFITAPSDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LML1</accession>
    <name>A0A5B0LML1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_012979</name>
        <name evidence="2" type="ORF">PGT21_013886</name>
        <name evidence="5" type="ORF">PGTUg99_022955</name>
        <name evidence="3" type="ORF">PGTUg99_028259</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000506">
        <property type="protein sequence ID" value="KAA1068230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000036">
        <property type="protein sequence ID" value="KAA1136033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LML1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036136984" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1068230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1085621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1136033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="10979" checksum="7B1612FBC1E300F8" modified="2019-11-13" version="1">MHISLASSMALVPFLHFGLVIGVSLKCGRCPADKAGFTLNPTGTETKYSCRVLSGYADCSIDIPCIQFHCEECGWAALRATWGCTAKHATPHLYYYIPGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LPF6</accession>
    <name>A0A5B0LPF6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_016110</name>
        <name evidence="10" type="ORF">PGTUg99_023028</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="10 13">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LPF6"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000130">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF103">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSIDASE MNL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="272"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1065458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1090865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="525" mass="59834" checksum="A6BD6B89135A6CCA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRH3</accession>
    <name>A0A5B0LRH3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glyoxal oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_030829</name>
        <name evidence="5" type="ORF">PGTUg99_029782</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRH3"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02851">
        <property type="entry name" value="E_set_GO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
        <property type="entry name" value="Galactose oxidase/kelch, beta-propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009880">
        <property type="entry name" value="Glyoxal_oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015202">
        <property type="entry name" value="GO-like_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208:SF96">
        <property type="entry name" value="GLYOXAL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07250">
        <property type="entry name" value="Glyoxal_oxid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09118">
        <property type="entry name" value="GO-like_E_set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glyoxal oxidase N-terminal" evidence="2">
        <location>
            <begin position="169"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose oxidase-like Early set" evidence="3">
        <location>
            <begin position="551"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1066450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1081944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="658" mass="71655" checksum="81029D81D800C2D1" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSI4</accession>
    <name>A0A5B0LSI4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000185">
        <property type="protein sequence ID" value="KAA1067079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSI4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="218" mass="24934" checksum="087A192F97816304" modified="2019-11-13" version="1">MTIHLFKHLWVIGNPTLHAGSAAGSRLARSLRKRPLSTLGYPFPPGVPDKHKHVTLVIIQAWLTMTKMIRHFIRYVGASLVLILHHINHHNYNSNKITSHERRQKAGDYHDDIKALMEKVREMQEAESSAENKDVRSDGDVYVSKPEEEEASEMANIYNSQKARIQHNTINRYRRRRKMDGRYGSQELHKKCASAGLLIQINTKGLGLHTAVFSLTHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LSK4</accession>
    <name>A0A5B0LSK4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphotransferase</fullName>
            <ecNumber evidence="11">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HXK1_4</name>
        <name evidence="14" type="ORF">PGT21_034597</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the hexokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="11"/>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LSK4"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24087">
        <property type="entry name" value="ASKHA_NBD_HK1-2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000156">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000009">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 11 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="12">
        <location>
            <begin position="142"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="13">
        <location>
            <begin position="346"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1066628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="597" mass="65872" checksum="1375C775184EEBD0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUT4</accession>
    <name>A0A5B0LUT4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_001361</name>
        <name evidence="10" type="ORF">PGT21_001847</name>
        <name evidence="12" type="ORF">PGTUg99_009257</name>
        <name evidence="13" type="ORF">PGTUg99_027593</name>
    </gene>
    <organism evidence="10 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="12 15">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Dispensable for SPC enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000206">
        <property type="protein sequence ID" value="KAA1123723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000005">
        <property type="protein sequence ID" value="KAA1137881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUT4"/>
    <dbReference type="OMA" id="IHLTLWT"/>
    <dbReference type="OrthoDB" id="263893at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009542">
        <property type="entry name" value="Spc1/SPCS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 12 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06645">
        <property type="entry name" value="SPC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1067344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1111481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1123723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1137881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="101" mass="11062" checksum="8246CAA5FF692F4B" modified="2019-11-13" version="1">MDALSKVIDGYVDQLKATFEGKIDFEGQRKADQLNNLILSLTTVIALVAGYALQSMLLTFGIYGAGFLTALLVVVPPWPCYNKNAISWLPSLKKQGKVKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWP5</accession>
    <name>A0A5B0LWP5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024761</name>
        <name evidence="2" type="ORF">PGTUg99_022877</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWP5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="132" mass="14394" checksum="4D682A4C55879C93" modified="2019-11-13" version="1">MSVSSWIDAGGMFLGNPSEGASNPRTTTIHQLDQSSSQLSHTPRAVNAEIASKSHLNDSCYYASAAFLPILLGIVPHFCFDIMSDDQRLEKGSGRGVRWRVSTKSAPTHSEYEINRDTPSAAKRISSRTVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWZ4</accession>
    <name>A0A5B0LWZ4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWZ4"/>
    <dbReference type="OMA" id="SQGHEME"/>
    <dbReference type="OrthoDB" id="2510539at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="446" mass="50694" checksum="C3B36CE54A33EA26" modified="2019-11-13" version="1">MLKSTKPESIPPNADQLISRQIDLLAQSFRSFKYKYDIYSNRRLRSTSTERIVPIDQASCQLILSRLESSLLPLLGHQISTISRIINSSDLRRGDTASKLELITELQLEIVQSVEQIFFCAPHRQLFYLSSQTDDNHLRNLKSFRLPKLLLKLGFLIHELSFLFDGFADLIRELKLSQKKYRRKARKSNTRGKVAEYTTDTAEAIECLIKSIKGHELSNIQEEWDVELTDMDEVLAGVTKLLNPTADSDEHEEVEDEGEFQEDEDVEDEGEEEEEEEDEDGDENGEEDDESQIEAPNEAFLQLARSTVPMIKLSRLFFRKLTSSRIDKSPSEPFTEMSTSQLETLTGAAGDIDGELSRILKTLSKAEETDEAHTARALLASVKKISKFFQSSMLLLTLYVLPLVPQVDGNPNSSQTNLKTWLITWNNLFISATETFISATHTFQMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LX01</accession>
    <name>A0A5B0LX01_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027384</name>
        <name evidence="2" type="ORF">PGTUg99_004859</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LX01"/>
    <dbReference type="OrthoDB" id="2507860at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="982"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="279"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="558"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="585"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="668"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="724"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1099" mass="119504" checksum="66D19C23D2754A1F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXC4</accession>
    <name>A0A5B0LXC4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016290</name>
        <name evidence="1" type="ORF">PGTUg99_035913</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXC4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1104249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="103" mass="11489" checksum="46E074BE5D2DE264" modified="2019-11-13" version="1">MSNQRVLGIHCLPKTLPALACQSQGFNGTKKLNGGQAIVAADPHIAREYALGLSFPRVLRYFTQSFQVGLSFPWVLPYFTQSFQAPESHQRQIQSRKVGFVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXZ4</accession>
    <name>A0A5B0LXZ4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_005151</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXZ4"/>
    <dbReference type="OrthoDB" id="2506191at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="57"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="277"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1068899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="643" mass="70889" checksum="291D1FECC53D9360" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYH0</accession>
    <name>A0A5B0LYH0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYH0"/>
    <dbReference type="OrthoDB" id="10586876at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022673037" evidence="1">
        <location>
            <begin position="24"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="635" mass="74164" checksum="0DE284E2721257C6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2N7</accession>
    <name>A0A5B0M2N7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025018</name>
        <name evidence="4" type="ORF">PGTUg99_007649</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000078">
        <property type="protein sequence ID" value="KAA1132572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2N7"/>
    <dbReference type="OrthoDB" id="2499047at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137153" evidence="2">
        <location>
            <begin position="21"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1132572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="871" mass="95941" checksum="7392BE4F16F2A31D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3A1</accession>
    <name>A0A5B0M3A1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine dehydratase</fullName>
            <ecNumber evidence="12">4.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Threonine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_001720</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="12"/>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3A1"/>
    <dbReference type="OrthoDB" id="4418812at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00054"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04907">
        <property type="entry name" value="ACT_ThrD-I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1020.10:FF:000001">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000008">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1020.10">
        <property type="entry name" value="Biosynthetic Threonine Deaminase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001721">
        <property type="entry name" value="TD_ACT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038110">
        <property type="entry name" value="TD_ACT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005787">
        <property type="entry name" value="Thr_deHydtase_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01124">
        <property type="entry name" value="ilvA_2Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006674">
        <property type="entry name" value="PRK09224.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF11">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00585">
        <property type="entry name" value="Thr_dehydrat_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51672">
        <property type="entry name" value="ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT-like" evidence="14">
        <location>
            <begin position="487"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1071265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="575" mass="62716" checksum="11BE8DB9D85A7868" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3L1</accession>
    <name>A0A5B0M3L1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025787</name>
        <name evidence="4" type="ORF">PGTUg99_013296</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000078">
        <property type="protein sequence ID" value="KAA1132607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3L1"/>
    <dbReference type="OrthoDB" id="2511481at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137134" evidence="2">
        <location>
            <begin position="33"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1132607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="443" mass="48317" checksum="F7212CF52756416C" modified="2019-11-13" version="1">MSSTFTNPSHRFFWLGMCILLLTHPGPTTIRAALVPASTLKPNQIVENGLDLGSQSSLCGPRVNTDLRLGSSHLPSVEGVRADIPDVEAGGNGKRKAPTQPRKKLMDPQLDPQELALSLAPPNAQVVSDVAQPKPDGASQLPAGVGSSTFPVRPGPNTSRPILNIAPHQSQPVPMRLVNVASPQDPQYQAIKAAAPAEKVTLAYNTPQRPGQNFLPNQHQHMSTYLANFGHPQNQAGSPAKKAKLAHNTRLPGQNLFPNQFKKLPSYLPNVANPQAPQYQVLKTGTSTKIVRLAHNNPQPVQNVFPHQFQATQAHLANGAHPQILQYQQARYDPNGMRYSHQTMQRIPQPGPEQYQAGPLNQVVRIGTPITNLNVLQSHPNAVQSQALYNMIQDQSKRIAQLGKRPITQAPVRKRQKKIPKGAAKLPEDLPLDRYMSDAESST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5H3</accession>
    <name>A0A5B0M5H3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_019185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5H3"/>
    <dbReference type="OrthoDB" id="2507436at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="294" mass="33731" checksum="AD773A1EABBD22B1" modified="2019-11-13" version="1">MSTTSINTSKGPTYRCWLCKGQSTKNRNRHCLNKQHQDFVQADNEQRSRVASRRAAARVSNEEVFVVNNNISQIESLVFEDQEDSQAEELESNRSEAIHEPNIVPLDIFEEEDSDNETDNAAELTWMQFLELAIGQISSEPDELADVDSIPSFRQEEVKIEDVTPDSSVWFPFPSKEYLIGSLLIGYLHKLISRDLYHQIRTIFTLYAIGLPGWEALRIMRAKIRSMSNHSIIQRESVFNQPMFGLDARQLISDDLMNPLVAPHLEFVPEDAQGLNIFKSSQSLKWLKHFESDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M672</accession>
    <name>A0A5B0M672_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M672"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1071314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="64" mass="6977" checksum="E7571022ED78077B" modified="2019-11-13" version="1">MLNPLLQQATPPAQESSTLPLGLLANRPLLPIASLLWEQCDRAVKCGPRPLFGRKSTPALLEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6Y6</accession>
    <name>A0A5B0M6Y6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CDC25_2</name>
        <name evidence="9" type="ORF">PGT21_025246</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MPI phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6Y6"/>
    <dbReference type="OrthoDB" id="26523at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF17">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00716">
        <property type="entry name" value="MPIPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="8">
        <location>
            <begin position="694"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="326"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="495"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="588"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="224"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="596"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1071999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="777" mass="84588" checksum="7FBB6F2C578E2ECA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8R1</accession>
    <name>A0A5B0M8R1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8R1"/>
    <dbReference type="OrthoDB" id="2506685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="418" mass="48700" checksum="6B89621D71C59E53" modified="2019-11-13" version="1">MSSSTLSPPWMRNKKTGNLPIFRTGIDKTLDMDEPIYQQPKQQEPVYQQPTNQNKPVLIEQPGLFYDGHHFMQFLRQYEMRADSLNASKYDRALQIGRFVRTEELKCQIESMDGYEECDWDILRASMFELWGDEYEIWYSTTDLVNLSEEFSRDNKTVSYQAFQTYLQNFSKILDFLLIQKQLRSRQDALVLFISSFPQELQRNIKRNLNQNGQLPQAPDGSRLPPLWEHLTEAAGVEIKLKELAKELESLKQQLESQSPVICNTPHPLSQLEPQTDYTALETDYTKPATEYTELATELTELATDYTELAINHTEPATEYTEPVTDYMELATDHTEPETDYTELVTPTIADTEKSIDVTIPVASMHMTEPVTTTKLLDLEEWKAASINMNLIRRTMWIRKKSWINQLNQKLWITFPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBN6</accession>
    <name>A0A5B0MBN6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Electron transfer flavoprotein-ubiquinone oxidoreductase</fullName>
            <shortName evidence="5">ETF-QO</shortName>
            <ecNumber evidence="5">1.5.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_010396</name>
        <name evidence="8" type="ORF">PGTUg99_027200</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Accepts electrons from ETF and reduces ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a ubiquinone + reduced [electron-transfer flavoprotein] = a ubiquinol + oxidized [electron-transfer flavoprotein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24052"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.5.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.1" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBN6"/>
    <dbReference type="OrthoDB" id="437331at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040156">
        <property type="entry name" value="ETF-QO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049398">
        <property type="entry name" value="ETF-QO/FixC_UQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617:SF107">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21162">
        <property type="entry name" value="ETFQO_UQ-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 5" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="ETF-QO/FixC ubiquinone-binding" evidence="6">
        <location>
            <begin position="138"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1073380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1122098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="285" mass="32081" checksum="C91B3E5C43777F3E" modified="2019-11-13" version="1">MTRALGELWPEWREDGSAPIVMKASSKSMKFLTARLAFYPPQMNNKGNYIASLSKVRWWLAKQAEALGVEIYSGFAGAKLLHSDDGKAVQGVITNDISLDRSRKPKDPEWSSMLTGSCWPRAAMTPRPTRDGKDPQTYGLRIKEVWRVKDDVYEPGKVLHTLGWLLQRDTYGGSWIYHLEDNMIVDDILDYTSLEDELGKTGNGSDLKAGLITGLGLFAWKQFNWTFGELVVRKFCNPGNLDLAKEMVHKSDAIHSSYQLASHHIHLYLSQLNSFNDSKAKAGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCB1</accession>
    <name>A0A5B0MCB1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCB1"/>
    <dbReference type="OrthoDB" id="2509948at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="8054" checksum="43EEF4184DE876B9" modified="2019-11-13" version="1">MTSGVNEIKKKKKKKKTKNSASNSSLTPPPLVISTAAVGSLTVMDENSLAALERQNDPRYREDLEFVLFEDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCR5</accession>
    <name>A0A5B0MCR5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCR5"/>
    <dbReference type="OrthoDB" id="10280720at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="386" mass="44424" checksum="3273FA323DC518CB" modified="2019-11-13" version="1">MSARQTAPDVDAELIKLIRTCYRFTPESNSQEEEQVSPEEDLKTRLEKLNRIESTILPAIRDLVTELLEALDLPAKDSISPQPKLDEASEIVPRLTSLVEDLACSVLPPDYSNPEICRLIDQNYGQLNQFRSCYFKKKITEFIKEDLRRLFSSLSSFVSDGEYYPPHYEPSDRDKPHQEGRAGRIQIIIPRIINIIRGSNFDIIQSIWNECEDLLASDINDIHWCINLTNTRAPARIVPDFSPLIPAVLEILDQTLVLLKMCRLFSRKIGSTRLFKFAEGISPADLASFGDVSEKFQIHVQSLIVYMETVAEFQEKPPGWNFKHEAEVISHDVHFCLDFIRSHMVPSYPPQTVDEVMDRLFGTFTQQFFPTLDRYIARLTQFQADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEN1</accession>
    <name>A0A5B0MEN1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_010016</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEN1"/>
    <dbReference type="OrthoDB" id="431825at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056750">
        <property type="entry name" value="RRM_ESF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25121">
        <property type="entry name" value="RRM_ESF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ESF1 RRM" evidence="7">
        <location>
            <begin position="152"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="6">
        <location>
            <begin position="609"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="184"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="392"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="465"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="601"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="484"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="539"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="650"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="666"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1074556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="694" mass="79021" checksum="CA7E1AD871321125" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MG42</accession>
    <name>A0A5B0MG42_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029631</name>
        <name evidence="2" type="ORF">PGTUg99_027160</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MG42"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="51" mass="6108" checksum="919AD7003135706C" modified="2019-11-13" version="1">MIIDGLEVQEASLEGYEDEDDEDDEDDYEDEYEDEEEEETQYLSDDEQMSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MI59</accession>
    <name>A0A5B0MI59_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028015</name>
        <name evidence="1" type="ORF">PGTUg99_020232</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MI59"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1075839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="102" mass="11046" checksum="64A08A2904EC621A" modified="2019-11-13" version="1">MFPIPAGSTRSAYPQTRSPIKKFNIKPQTVSFSTGTATSDIARDIHSPTDPGFGGNSNLLPPLFIILGFKGGFAARLNNAFLSSSAYPLPSTLVYRKNKRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MM06</accession>
    <name>A0A5B0MM06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020704</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MM06"/>
    <dbReference type="OrthoDB" id="3237105at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="348" mass="41247" checksum="E11A0929C6E8202F" modified="2019-11-13" version="1">MWSYLSPLVSPLRYATRNHRLAAISHRLKHHNHRSIQQLPYWLQRKFCQAVRRRRENQNLLDQLLNKRNRHRQGGGNFTIGFFKRQWAAQREFQSNHTTEEDTRRSKLLSIYKREASINLMRTRLRNPRDLLEDPGEIQELMDSIVEEANLLRQEKEEMGVANMPETTDTEEQKLRLLLWDAKSALFVQAVHINAERQPLINSHTMGSRLGTRGKEKIVKASQARRPAVQKLIDAYNQQFRQFKAKYPNQQLSDEDDHPVTYDEFSTWPMDHRFWNDGLYYHSSEPWSVDPDVKTGINCVLMLSRTQEEFELIAQELARATGWAIDHYKLIKNKLLYIEIREFLLPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP50</accession>
    <name>A0A5B0MP50_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020513</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MP50"/>
    <dbReference type="OrthoDB" id="2536450at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862:SF1">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023010813" evidence="2">
        <location>
            <begin position="20"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="302" mass="29326" checksum="7A8116FE52BB4D14" modified="2019-11-13" version="1">MFSLSLSTTLLVLSMGGNQLVVNGQAATNASGSTTREGGAGSSSSSPSSAMGLASGLSPGCQAAAGGLLTSDFGACSNIMGLVSVIGASGSVIPPLTNWISGVCSASPCSADTLKQANQTVSSGCSSDIQKGSGIAMALQTIVSNYNGAKDLLCTQYTSNSTFCVPSILGNVQTATGQNITISEVTSLLSGSMTPAGQAFANVPSGTYCTDCGHALVTQSAAFLASSNGGGSSGNASSGGAMSSISNTCGASFTDGKLPSTVRVANKNAADNAKNSAAVRLPGSLLIAFSVALSASALALLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQG3</accession>
    <name>A0A5B0MQG3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">methionine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_032141</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQG3"/>
    <dbReference type="OrthoDB" id="5844513at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000037">
        <property type="entry name" value="Methionyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000738">
        <property type="entry name" value="WHEP-TRS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SMART" id="SM00991">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51185">
        <property type="entry name" value="WHEP_TRS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WHEP-TRS" evidence="14">
        <location>
            <begin position="668"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="454"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="619"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="640"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1078266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="724" mass="81152" checksum="BF89BD810940503E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MQH0</accession>
    <name>A0A5B0MQH0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_003013</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MQH0"/>
    <dbReference type="OrthoDB" id="2502798at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF85">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G00710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="43"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1079185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="444" mass="48941" checksum="FFD4B34646588EC6" modified="2019-11-13" version="1">MMKEEEQAATPGQTMNQKEKPGLSDASTSPLERRALLKLDLFIIPMVTLCFFFSYLDRSNLGNVRIVGLQKDLQMSDYDFSMALTVTFIPYILVELPSMLTMRRVGAGIQIPLTVIIWGVVTFLHGFVKTYRGLLVARFFLGLVEGGLYPGLILYLSMFYNKAELQLRIGLFFSTMCLSGVVSGLLTYAIVKLDGRWGHPGWSWVFMIEGIVTTIFGIITLLFLPSSIKKTKFLTDEEKTILIARLERNNGSNTVSSTTLTERFPAKSTSQQVWEAFKSPHVIMLSAAQFASANNISSLAYFTPTIIHSFGYSPTATQLYSVPPLSLAFLTVLGVSYLSDRYQVRGMATICCAALSALGFAIFYASSDYRVRYGSLFISSPGAYAVMPTLSAWASNNSEPYKRKASTIGLGSMAGSIGSLLSVWVFVLGRPPRYHLPTGLSLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRU0</accession>
    <name>A0A5B0MRU0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FMN-dependent dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000135">
        <property type="protein sequence ID" value="KAA1078814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRU0"/>
    <dbReference type="OrthoDB" id="25826at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN-dependent dehydrogenase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1078814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="109" mass="12873" checksum="2894897EE595D05D" modified="2019-11-13" version="1">MGYYSSGSDDEISLRENRAAFKRVWFRPKNLKRCKTDRLFVSITRFYSTRKTRTSRRRKEPDDCAGQEGINSNGYPTLASCAFEELVQARAESQNQWYQVYVNQDREKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUG2</accession>
    <name>A0A5B0MUG2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUG2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="355" mass="40783" checksum="863C3CE709141A51" modified="2019-11-13" version="1">MCTEMQNDLKDLNEKLVAVLVLQPNSPIDRMTFVDQYFPHLSAKAAFKKFNFEERVILSSATIYRNEKYQELSNRLEVLELELTIELLPAGQSFFSLPMDEHVQNVQRKVSTNNHYQNLQSKLLQMRIKRRHLEMQVGDNGQRGMMFPALWRIQPQKQATSTITASENDEELHRIIANQTTSTALMCTAMKHDLKQLKEKLVAVPVFQPNLPVNRMNYVPAKAASEAFDLDERVVLPSAKMDPNELYQELLGHLEVHEAELTMKLLPAEHTMFLSLHQVAQRVERKISSTDSYQTLQANILQMRIKRRQLELQVKNHPSPTITCTVLPDQILEVNDEVFSSDTSGSSSLIYPKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3K6</accession>
    <name>A0A5B0N3K6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S9A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_023844</name>
        <name evidence="4" type="ORF">PGTUg99_020476</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1083044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3K6"/>
    <dbReference type="OrthoDB" id="248387at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070012">
        <property type="term" value="F:oligopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.120">
        <property type="entry name" value="Prolyl oligopeptidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023302">
        <property type="entry name" value="Pept_S9A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051167">
        <property type="entry name" value="Prolyl_oligopep/macrocyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881:SF2">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02897">
        <property type="entry name" value="Peptidase_S9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9A N-terminal" evidence="2">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1124020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="140" mass="15635" checksum="EBF7A9CA5B6E0779" modified="2019-11-13" version="1">MSWDEYPLIGRNPSRQEDPYSWLHKPPKQSNALKRATDPKQPAGERFLDPNLFSINGTTALSFSASSESRIYMVYGVSRSGSNSQTIYVRRTDSPHTKSAADGGKRGENPGRMEDTVWWRERALTKALAGLFGGMRNARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9A5</accession>
    <name>A0A5B0N9A5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021713</name>
        <name evidence="2" type="ORF">PGTUg99_003288</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000386">
        <property type="protein sequence ID" value="KAA1090373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N9A5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="69" mass="7590" checksum="FE85C8D9A0D595AB" modified="2019-11-13" version="1">MGGSRAIGDDYRLQHELSGSLSPYMEPRLATRFRAFSDADGVPILRLQESLISLSVSSQTMNLIAVLCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAG2</accession>
    <name>A0A5B0NAG2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NAG2"/>
    <dbReference type="OrthoDB" id="2511178at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="568" mass="64459" checksum="FCC698A697AEFB7D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI24</accession>
    <name>A0A5B0NI24_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_002805</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI24"/>
    <dbReference type="OrthoDB" id="419616at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF15">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="58"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1088975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="526" mass="57550" checksum="0A8569F27E2BDBD0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJB6</accession>
    <name>A0A5B0NJB6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013811</name>
        <name evidence="4" type="ORF">PGTUg99_020657</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000408">
        <property type="protein sequence ID" value="KAA1088028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJB6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137467" evidence="2">
        <location>
            <begin position="25"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1082761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1088028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="189" mass="19409" checksum="89E70B29938EC630" modified="2019-11-13" version="1">MQPIAITKISLAWLTTCLTYYVMAAPMKGIGEVPLKAGCPCGHDAEKIQPLVIKDSPSLVVAEDRNVIASGCKGGHDHDHHHGPTPIVANSPTPPPTPQEKPTTNGSCASGCDHHDGPVVIPSGPTPPILNEMPSTQSACGSGSGHDHGPVIVGASDPHSHGKSSEGQSKNVSEAPKAGGCCHDHHHGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNN4</accession>
    <name>A0A5B0NNN4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_016962</name>
        <name evidence="3" type="ORF">PGTUg99_026021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000510">
        <property type="protein sequence ID" value="KAA1065484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNN4"/>
    <dbReference type="OrthoDB" id="2506293at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137493" description="Secreted protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1065484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1090885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14083" checksum="6BBA1B4FBF614293" modified="2019-11-13" version="1">MFLTWSVIFVLLMVFKQECSAMPSEASYEVGTRDKTAEGPVRCLTNRCSETEANGFRRDEPEPIKRRPLRQSAPIKCGGTGLPPPCGPQPKRSEPLTVPVKSNPSKGPPKTHQTAPVTCGDTKLPPPCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNQ2</accession>
    <name>A0A5B0NNQ2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNQ2"/>
    <dbReference type="OrthoDB" id="2509708at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="400"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="553"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="596" mass="63092" checksum="24329B3567CB13E2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NPY6</accession>
    <name>A0A5B0NPY6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030532</name>
        <name evidence="2" type="ORF">PGTUg99_030088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NPY6"/>
    <dbReference type="OrthoDB" id="2499640at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="117" mass="12380" checksum="43E57F08F687E356" modified="2019-11-13" version="1">MCPIHEKDHPICPTGAPSRDFSHHRRPPPKPVAKPRPTLPSSGSARPQGMVFSPDDLPQALKNIGGTGLPPASGRLPAPLSLQARASRGKGIVFNVDELPQDLKELQSAQGNKTNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NX48</accession>
    <name>A0A5B0NX48_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025411</name>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NX48"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1093104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="8554" checksum="6A0F8508254ABA78" modified="2019-11-13" version="1">MPKDHRSSSKIVYGAPGALSKVKPDRPRLFCACMLECLSDILRGGAISQPGILGEQIDVPYDLLGMAFPALYKLYLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NXQ5</accession>
    <name>A0A5B0NXQ5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016795</name>
        <name evidence="2" type="ORF">PGTUg99_032412</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NXQ5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1092870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="96" mass="10769" checksum="55C80A5CD3D14775" modified="2019-11-13" version="1">MEEKKEQELQIMKEKAENTEKMNAVYGFGTPLNGFLKTKAGNAYLQQSTKSNVNYLGVAMLQNQQQATWLSQPALLQFLKITFHSSPPNSTCPKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NY36</accession>
    <name>A0A5B0NY36_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013262</name>
        <name evidence="1" type="ORF">PGTUg99_016384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000515">
        <property type="protein sequence ID" value="KAA1064173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NY36"/>
    <dbReference type="OrthoDB" id="10304334at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1064173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="112" mass="12608" checksum="B5D7A145FBF90820" modified="2019-11-13" version="1">MREFSIQSPNNGTVLAAATVASHQVINSHLEAQEPQRALGNIEALLERMEARLNGRSVLREERIITHIDETSQAMEQLLTAQIEAKTTLVEERIMAHWDERFEEVNATMAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NYK8</accession>
    <name>A0A5B0NYK8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NYK8"/>
    <dbReference type="OrthoDB" id="2507749at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="627" mass="66617" checksum="C28DE6CCBB0DBCB0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZ74</accession>
    <name>A0A5B0NZ74_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting/targeting protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Carboxypeptidase Y receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 15">Sortilin VPS10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">VPS10_1</name>
        <name evidence="21" type="synonym">VPS10_2</name>
        <name evidence="20" type="ORF">PGT21_001115</name>
        <name evidence="21" type="ORF">PGTUg99_004372</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 22 23" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">21-0</strain>
            <strain evidence="21 23">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Prevacuolar compartment membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS10-related sortilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000051">
        <property type="protein sequence ID" value="KAA1134555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="443634at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15482">
        <property type="entry name" value="Sialidase_non-viral"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000690">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000801">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000005">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000007">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.80:FF:000001">
        <property type="entry name" value="Sortilin-related VPS10 domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.80">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="Sialidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1452"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="19">
        <location>
            <begin position="106"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="19">
        <location>
            <begin position="814"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAA1093864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAA1134555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1564" mass="175477" checksum="5803B6493462461E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZE0</accession>
    <name>A0A5B0NZE0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_008988</name>
        <name evidence="2" type="ORF">PGTUg99_033526</name>
        <name evidence="3" type="ORF">PGTUg99_033567</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZE0"/>
    <dbReference type="OrthoDB" id="2501290at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="673"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="689"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="699"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1094076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="801" mass="87612" checksum="E6D0D27C460116EA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZJ1</accession>
    <name>A0A5B0NZJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sphingosine N-acyltransferase lac1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LAC1_1</name>
        <name evidence="7" type="ORF">PGT21_011494</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZJ1"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13883">
        <property type="entry name" value="CuRO_2_Diphenol_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13904">
        <property type="entry name" value="CuRO_3_Diphenol_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF414">
        <property type="entry name" value="ADR239WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="4">
        <location>
            <begin position="86"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="361"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1094152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="514" mass="55564" checksum="5A59F43DDC9DC7A0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P273</accession>
    <name>A0A5B0P273_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Adaptor for signal transduction</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">STE50_1</name>
        <name evidence="3" type="synonym">STE50_3</name>
        <name evidence="4" type="ORF">PGT21_011781</name>
        <name evidence="3" type="ORF">PGTUg99_029876</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P273"/>
    <dbReference type="OrthoDB" id="8883818at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="42"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="825"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="863"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="825"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1094159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="926" mass="101344" checksum="5FA173EBD826B75C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P7K8</accession>
    <name>A0A5B0P7K8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P7K8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023124798" evidence="1">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="125" mass="13880" checksum="35AC880593D58A5D" modified="2019-11-13" version="1">MTSIFMLLALLQKKCRLVDSDSIANDLPTPLIADGITNAYLDTDRSFLALLFMTTDLVRLATSIQIAPFLLSSDQFTTIMTYRLDSTPRVSPNDFDTPKTTPLSTPLLYIITPSPSSCRLIDHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAY3</accession>
    <name>A0A5B0PAY3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Eukaryotic elongation factor-2 kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">EEF2K_23</name>
        <name evidence="2" type="ORF">PGT21_029879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAY3"/>
    <dbReference type="OrthoDB" id="2500644at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903013">
        <property type="term" value="P:response to differentiation-inducing factor 1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF2">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="428" mass="47787" checksum="AA8AEC8B13DE4BA9" modified="2019-11-13" version="1">MSDMLKCAGCNQWSKSRHFENHCLDCIVSRIQAPSTQPIGPSTSHTQIHQRNLHTAGPSYSPISRHSVNTHDTGTHVFYQGLAKEARASKKREISTPKPRRASSSRTPDPKPSPEKWFVKAGVTVYIDDVLQKKTGIIPKVLEVDTKKPNFYEDFRHQLWGLFSDEILAKTEITFPPDPEQYTCLGTNNSKIEEHNILLELARAGKTIKSASVIHLIYQHPMDNFPETDIPTQSRTNQKRKYQGDQSKASKSAAWALGGKIATKPVRGSASLSSQVDTLSHPISSQSITMKTDGWIPALRLHFYTINEQPPPEASHQSFTINDYIHAVTFAFDIKVDEGKVLGEGSSRCSYPAQVRSIENGREVINKWVAKLRYSDSTPNVINHASDAITYRGFGLILAEFKDFISNKLDGVLKVKGNQIEVSCVSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBE1</accession>
    <name>A0A5B0PBE1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBE1"/>
    <dbReference type="OrthoDB" id="2508473at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="112" mass="11885" checksum="9D330836F31C2347" modified="2019-11-13" version="1">MATLIASFSSSLTGAGWSKSLQPCWFNVGLMERPTVGSTSVNHRLPMQTLAQPSMSVVTPSVQPKDGWANGNGWPPLAGPLSNRSPNLTVGSELRERSATSYVGSTSTNHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEY7</accession>
    <name>A0A5B0PEY7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030485</name>
        <name evidence="3" type="ORF">PGTUg99_021640</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEY7"/>
    <dbReference type="OrthoDB" id="2512247at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="206" mass="23466" checksum="4935E540A3C926E3" modified="2019-11-13" version="1">MATEDPHITQADRPDGQTGDPEMLNKTHDLLAVPMPKSDKAILWDKISAAVAINDKDNADFFIRLYSQLSNSNATGKPEILRAKTSDAVTPLTTASSSKKTNDKTSIIFVRGSLPKHFDVGFTPYFDKNIKELRGPIPLTIFDKKWQEDAIQYHTNKRSKGDEKDGNYAGFEYPNEWTQTFAKWTANHRNFHVTFRDMYNYPEFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PH46</accession>
    <name>A0A5B0PH46_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032540</name>
        <name evidence="2" type="ORF">PGTUg99_010115</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000342">
        <property type="protein sequence ID" value="KAA1099169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PH46"/>
    <dbReference type="OrthoDB" id="111386at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1084600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="138" mass="15862" checksum="8BFCF477855DDB79" modified="2019-11-13" version="1">MTTSTRRTPWENNRQPGGPDSLSVLFNWLKGGNHYARWRGGQNKTLFYWDLLAEFTKHGIHHHTRSDANVMLSYLQMSYNAGSHFLTKNGDPLALGNCSRDHPTIQAQLRQIFRHWDHLNEFMEVGPGPTSAWEITNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PM65</accession>
    <name>A0A5B0PM65_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_029147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PM65"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF8">
        <property type="entry name" value="NUCLEAR MRNA EXPORT PROTEIN THP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="57194" checksum="E03BDED2FAF36524" modified="2019-11-13" version="1">MRAFPSQKRKQGSSSLELEELTSEQLVERFADHLRHQQDSLSELVRPLDRSLIRLLKSKLSSNRKDDLDSLVQRQLGENRTLADFLSNYFTYIMLVQFEDEPAVINPGFHHYQKQTDPIIDHDNNFDLLLNAYNPASNIFRRPDAAYFTRSIQHLSHGLVYFAIKADRKKRSTKKEKASEAARQMTTTLGVACIDRSPEEPSKRRAAFSLANGLFKIYFFLNNMRLCDTVVKNISNVLNQLETHYPKAELVTFHYYLGRLALYQRRLHKARESLKKAFDLCKTDSWRNRRLILTYLIVASLPLGILPRPILLEQFQLQNEFHEVVGSLRTGNWPGVVNGLEKNRDWFRYKGIYILLREKLEVICWRNFFVIMAGLKNGNPGMRLSLTQSVEAARKVFMEPSIDEDDIVCMASSLIDQGYLRAYIKLGEMIVFGSVLPQISTVGEHMNEGGPEPLPAFSGKAIPMSIQPSSDSDAPVKSYRRPYSIRGAKKISHTKRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNT8</accession>
    <name>A0A5B0PNT8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_014950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNT8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022697525" description="Hydrophobin" evidence="2">
        <location>
            <begin position="25"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1103337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="13718" checksum="A70A78D94D31CA32" modified="2019-11-13" version="1">MFTFNLMPIVLAILLQGQAIMVQGFECGASARPVGVCRIHRPPTPQPGPGSGGSTGPGGGSGEHHGVPKIYIDLKPAPHSNNNHFACTGPTTGPNRVRLYCCPRNKNKYDITNLALTTFLYHYGCEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PRE9</accession>
    <name>A0A5B0PRE9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PRE9"/>
    <dbReference type="OrthoDB" id="2498564at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="578" mass="65359" checksum="E9FB25B5A8277C18" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PV72</accession>
    <name>A0A5B0PV72_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA polymerase II transcription factor B 52 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TFB2_1</name>
        <name evidence="2" type="synonym">TFB2_4</name>
        <name evidence="3" type="ORF">PGT21_027122</name>
        <name evidence="2" type="ORF">PGTUg99_008250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PV72"/>
    <dbReference type="OrthoDB" id="2507768at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="318" mass="35610" checksum="97B597D7CB480B43" modified="2019-11-13" version="1">MDQQQADHPMRSLLDKTYASSTSTQNIAILSPFVQLSFVFHLAIAQLQRHIQETPDCVVTVIGSSSQAAFQNTLADEHDAVLFTDSSSDCLTKTLLERIEFRFCADLAQLLTLLCELGHSTVERPTLNNDEEDRGMRSPRIGLIITDLTSHLISLPTPTLSDLTRLLGFLSCTQTRLPSIHHHHHHPLQNPPPIYLVDRVDHNQKWPLSTPTADPEDLRNMLEIFHHFFDQIWTIEGLHETDSNTGDDSPTTTPTAVGTSSSPKPGRYRLYSLRSAADSSADDDDQITYQVLEQEALDPEYSAHPERLLRKTVVVPGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXP9</accession>
    <name>A0A5B0PXP9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MutS protein msh4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MSH4_3</name>
        <name evidence="8" type="ORF">PGT21_011467</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXP9"/>
    <dbReference type="OrthoDB" id="276261at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.110:FF:000003">
        <property type="entry name" value="mutS protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF21">
        <property type="entry name" value="MUTS PROTEIN HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0469">Meiosis</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="7">
        <location>
            <begin position="675"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1105549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="722" mass="80221" checksum="6A50F23850DD5847" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PY09</accession>
    <name>A0A5B0PY09_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PY09"/>
    <dbReference type="OrthoDB" id="2517957at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="12400" checksum="DFD4A143215E1CB5" modified="2019-11-13" version="1">MARSSVSPCGTPSQTPSRQSSQITTPLRSHGNFVRSNQDSRRSLQPQTSRQIQTPNPESETESVSQTKSTQPFQKQSRKQHRPQISSLTDQSHASAAKSKQKKKKKKKNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZN0</accession>
    <name>A0A5B0PZN0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034434</name>
        <name evidence="3" type="ORF">PGTUg99_033876</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZN0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474298" evidence="1">
        <location>
            <begin position="24"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="250" mass="28785" checksum="17AE8AC963E06E24" modified="2019-11-13" version="1">MKLNFPIQYLVLLGLATVQFSLSNINEPHLDLDGIEYTRPAKKIKMNEETGSGKSIETTQIPNDIFLKNNLDNKTYQEKCKKNEAEMQEMERGILEIRLNIEWLKKHEKSKFSEFAELLDKIDSNLKGPILEKLVELKGRRPFSSLIAWYGGVSIPKQNTQFKSTGTNEISDHTFSIIKECFMAELVSIWNDLNKNIRKWKDGPCETGEGIMGVLVSTKCMFLIENFIRTHKLISPNFLEKIKTFVPESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q3U4</accession>
    <name>A0A5B0Q3U4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018387</name>
        <name evidence="4" type="ORF">PGTUg99_017250</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000307">
        <property type="protein sequence ID" value="KAA1107778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q3U4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366494" description="TNFR-Cys domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1107778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13069" checksum="2580ACD82A7A8E56" modified="2019-11-13" version="1">MSMNLFKISLSIAVAGTLTDMCVCPSKCAWCGKRMYHFPVPPYPCKETCGEVDKRTNQECLLLRTKQSHRCVSCKELTLQNMGSLDASEGCRHRNPQKLVSSPESSTSTPVELDLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6A3</accession>
    <name>A0A5B0Q6A3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peroxin/Ferlin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_019447</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6A3"/>
    <dbReference type="OrthoDB" id="2505878at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052646">
        <property type="entry name" value="Peroxisomal_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="7">
        <location>
            <begin position="388"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="224"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1108619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="426" mass="48722" checksum="26EFA810CB186807" modified="2019-11-13" version="1">MPMDAREPKRKPETSRRIEALSLGQLSPLLATIDELLSLLTWTHPAGHRAPILLLLIHAAFSLLIRPPTGFVDLLLLPLASAALLALKHHRRLRLLPPHIRRLAGLDLANRIALGFLNLALYLSISSRSLFSVLNTHPRSSLLSFFFLFYLIPFFLFPHLPASPSSFLLPLLLSWNSPPLRWLRKLVSSSAWYRSYGSLDHLDRFKTGNRPIKSRCSYSKTTIEEQQQQAHLQEQGQEQEQNPEHPTSTTTSPSGTATARPEKRMLIIRIKLLIIENQRWDQDHGWSSELTREDTRLVGGQWTDMYGQEVCKPKDIKLPPPPPRSEQDRWASIEGEGGWRWDDREWSVVRPDHPRHHDHLSSAPLFLRLAFGPLLSSAHPAWSVDQHGWTYGDHSWTAFSDSPRFNSFVRKRKWTRVVALTAPLCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9J0</accession>
    <name>A0A5B0Q9J0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Adenyl-nucleotide exchange factor sse1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SSE1_2</name>
        <name evidence="6" type="ORF">PGT21_031274</name>
        <name evidence="5" type="ORF">PGTUg99_033581</name>
    </gene>
    <organism evidence="5 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9J0"/>
    <dbReference type="OMA" id="WEQSPEI"/>
    <dbReference type="OrthoDB" id="434160at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24094">
        <property type="entry name" value="ASKHA_NBD_HSP70_ScSse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF4">
        <property type="entry name" value="HSC70CB, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="723"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="736"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1109742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1119666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="783" mass="85701" checksum="C102279A61CA0EFE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9T0</accession>
    <name>A0A5B0Q9T0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">superoxide dismutase</fullName>
            <ecNumber evidence="4">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_008495</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9T0"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000007">
        <property type="entry name" value="Cell surface Cu-only superoxide dismutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053257">
        <property type="entry name" value="Cu-only_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20910">
        <property type="entry name" value="AGAP001623-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20910:SF1">
        <property type="entry name" value="SUPEROXIDE DISMUTASE COPPER_ZINC BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022735734" description="superoxide dismutase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="8">
        <location>
            <begin position="52"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1110056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="184" mass="19218" checksum="C6BAF0313C1591AA" modified="2019-11-13" version="1">MRSSFISGLVCLSLAKYSVGLPQLPLLQSARPFQPIQARAILLSPTGAPIFGVIDFRATTLTEVQVDVVVNGLEAFVPQANHAYHIHASPVGPDGDCAAAGAHLSAAGIPDSVPCNPMMPRLCQEGDLSGKHGVLPGNQRVVQSSYTDSTLQFLSPESGIIGRGLVVHDAKGARIACGNITRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBJ6</accession>
    <name>A0A5B0QBJ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-terminal acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NAT1_2</name>
        <name evidence="2" type="ORF">PGT21_023240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBJ6"/>
    <dbReference type="OrthoDB" id="10260017at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF0">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="336" mass="38694" checksum="E9DE94F87759CBA2" modified="2019-11-13" version="1">MMAQAESNDRLFTSNEIKEYLERIGLDQQEINALSPSEKLRTLHLNHILNVPFDTTSIHLPAKWWMMESTNTEEQAITLDRKNRKINPELVSVYCDHTFNNIIKERRGGYCWSLNSCFARLLLSLGYQVSQLPARVYNHRNQDPKEAGYSWTPLCHECLLVDTGSAKFVCDVGFGGGSSAYPIPFEENREIDTLTVGEKFKLVKEKPTDLDTSSTNQTEGYTVQRWCGKWWSPCYHFYLSPIVFKDLAIFNWHATSHPEAHFQTRMFVSLLQKDGSRRTLSCIIDDDGHQNQIKLHTRDHVGASESEVSYVQPTYAELFKTLQREFGWGPSQINTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QC93</accession>
    <name>A0A5B0QC93_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033308</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QC93"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023025136" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11410" checksum="147DA10B2585127E" modified="2019-11-13" version="1">MLIKKELCFLAIPCLISAQSVKIQPQIEAQECTHQFRIPAFGDFPCPDVFNCNDGHPHVCNHQRDGSSQRCTNCDDDDEIQFNQDCPHDPHPMRSCPWAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCJ2</accession>
    <name>A0A5B0QCJ2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450-dit2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_034817</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCJ2"/>
    <dbReference type="OrthoDB" id="2498889at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1110946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="45620" checksum="F51C6CF43B1DE0C2" modified="2019-11-13" version="1">MSSSHLSQVTPKITNSVIELIQLWKKRSQILDESGEKYFKAAQDLRLSTMDTISDIIFGKPFGVMSARLAHLETSNGALSADARPQFPTLARALNVIVAEVARCYLSPSKSLFWFIQRLFNREWRQAKSTVFGYLKNEVEKERITLSEEQIFGGADSKDPDNVDSVLSLLVKDEHLSKLRGEKPLSTDEITQELLVYWVAGHGTMACTLAWAVKLLSNNPEVQYKLRDQLVNTLPSLNERSPTFSDLKVCSSDLGYLDATCFEILRCGKVLGEVSRTSRVDTMVMGHVIPKDTVVMMPHSRLSSPQESESEFRPERWIDGDGKFDAQLPGPLHVFGHGQRGCFGKNLAVLELKLYIAMLCMELFFDRVPDDVNSMDAIELVANHPQTCVIRPIPWSKISNDSTQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QD50</accession>
    <name>A0A5B0QD50_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QD50"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="207" mass="23865" checksum="E4DEEA992F1B4E32" modified="2019-11-13" version="1">MEGVPDFLQRRFPHHKIKQIHQLRLLQHDVLKKDYFVLVKKNTSSGSTKDIECVESIWSASLEHQTRYFVRARRFLQGPINPFYQMRELDVTSHVDYFEASDIVACLNTQHNCQSGRCQVVKGSRNKGPNYEGTQTTLKIRHNDKKSFILNSASLQDPVTHRELAGLNTYYHLNWATAIETGRARWRPNPTNQTSQTRASSLAPSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFU2</accession>
    <name>A0A5B0QFU2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="5">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PDA1_1</name>
        <name evidence="7" type="ORF">PGT21_020021</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1112012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFU2"/>
    <dbReference type="OrthoDB" id="10256198at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5 7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="6">
        <location>
            <begin position="123"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1112012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="456" mass="50653" checksum="E283E74A2B8FDA60" modified="2019-11-13" version="1">MAHLTTNAFTRRFLTATARPTVNKLNLSSTRAALSSLAQYPQHRSPKAQSSLKSAAPLSTLNSRQIQTAADSSAVNPDKIPSDDGEKFKITLDADYYQTYKCDAPSLELEMTKAELVQMYRWMVTMRRMEMAADALYKQKMIRGFCHLAIGQEAVSVGMESAIKPDDKVITAYRCHPFAVLRGGTIKGVIAELLGRKDGMSSGKGGSMHIFTPTFFGGNGIVGAQVPVGAGIALAQKYLNQDDKHATFIMYGDGASNQGQVFEAFNMAKLWNLPAVFVCENNLYGMGTSAERSSSNTKYFTRGDQIPGLQANGMDVLSVHNACKYAKEWTTSGKGPLLLEFITYRYGGHSMSDPGTTYRSREEIQHMRSTNDPITGLRNRLLEWNVIEEAELKAIDKQAKAEVDVAVEEAKKSPEPNPETDMWTDIYYKGTAPKWMRGREKEEVHRYKPEECKDLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QG92</accession>
    <name>A0A5B0QG92_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019928</name>
        <name evidence="3" type="ORF">PGTUg99_013836</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000282">
        <property type="protein sequence ID" value="KAA1112165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QG92"/>
    <dbReference type="OrthoDB" id="2510491at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137963" evidence="1">
        <location>
            <begin position="22"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="115" mass="12678" checksum="E0446BA2CF93F192" modified="2019-11-13" version="1">MNTSTLFKFLLVVLIQGEASCANQVPKPAPKVPQSFSCGARAPVCAWKVFVDPKAINPGQGDRVELSNAHNNHDGTWSCRGKYTMQSCCSLPVRDKSRFAIEIWQKECPDPANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ63</accession>
    <name>A0A5B0QJ63_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Uracil-DNA glycosylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_022125</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ63"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097510">
        <property type="term" value="P:base-excision repair, AP site formation via deaminated base removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003592">
        <property type="entry name" value="PRK05254.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264:SF0">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00130">
        <property type="entry name" value="U_DNA_GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="7">
        <location>
            <begin position="157"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="286"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1113129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="483" mass="52247" checksum="FE642D27523AFC00" modified="2019-11-13" version="1">MAPAAASKPDKRQRSIAQMFGAKDPTLSGTSKSSSQGASSSTTSSSRVTGPASKAKSKASNVKTLMTAGRLARVSYQKLQPIDPESYRDSLSAEFKELLALELDPEHGLGPTYLHVLRAQLTQQYFLDLKRFLIAEGLNQPRNDIYPTSKNIYHWSRATPLDQIRVVIIGQDPYHGPGQAHGLSFSVPKGIPIPGSLRNIYQELKSEYPEFKAPGHGTLRSWAESGVLLLNTSLTVKKGAAGSHSNRGWEQFTDAVVDAIDLYGGLGLLNNQKPSEVDQIVEPASKRMKLDHPPSTEASPPQAKGPETGSDSDPKAPSPIAYSTPMASISDPKHTTSAPEPEKTNLKPSTEDLNTLASVDKKSEESQPARPDTNSAESGSKAKPPEVAGADVEPPSTKLTEEPKPQDLPGFGKGVVFLCWGKWAADRVARLSESKHLILKSAHPSPLSAHRGFLGNKHFKLANEWLENKYGESAKINWCDLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QKB5</accession>
    <name>A0A5B0QKB5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030978</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QKB5"/>
    <dbReference type="OrthoDB" id="10659988at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1113414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="462" mass="52702" checksum="ED8BE05627C0450D" modified="2019-11-13" version="1">MTSSLTEAHMEDPCGPRTKILEYGDSVIDGFRALTEKYAREDCECPYVRPDMPITYQEHRLSDEQVEMKKALLDSLHSSLLPRLRQQISTMSLSLEPSNLKEDPTFHLQRILALQPEVDKTWDQIQSATLSVSKVLMKFGNDQYLKELKSFRLFNLRTHFIKNLSGFLSALFSESVDSIRLLNLSNQKIPRWCRYMCSAWKSEYGHSVLKAIDSTLAWIKASEYEMVQLNWPSHVSVMDANLKKLLNLIDSIQTHSLNKQSRLTPARQSLMVPIKLAESILPIIKLTRLLLLKKLSRSGISNNKKTPSPFMELCSGQLQSVNQLSQSLDWDCEELLRALKRCNDTVIGDPDAGSDSEEEEMIVNAHNCRSTLCLRIAELANFLPLRVKMPLTIVSRCFLPPHPPPLLNNNIEGFQSSQLDHPIAPWINTWSDQFGLAARRLQVVNRLFAPRFVLDNSLLDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMV8</accession>
    <name>A0A5B0QMV8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycoside hydrolase 131 catalytic N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_004911</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMV8"/>
    <dbReference type="OrthoDB" id="5283326at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041524">
        <property type="entry name" value="GH131_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612">
        <property type="entry name" value="GH131_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612:SF2">
        <property type="entry name" value="GLYCOSIDE HYDROLASE 131 CATALYTIC N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18271">
        <property type="entry name" value="GH131_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023060047" description="Glycoside hydrolase 131 catalytic N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 131 catalytic N-terminal" evidence="2">
        <location>
            <begin position="62"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="328" mass="36418" checksum="CFADC697FB1E360F" modified="2019-11-13" version="1">MQQILLLSVLLFASHCVGEEPARTELAAGNSVPKAGKYAAPTRKSVAKAVQRLFDFALTPDMKTADLDVPKSAVNKLIGYVVKGPAKPSAYLSILHPSPTKIQLAIRDDSIFIPGGKPADAQRGFRRTDVNPAIDKTTTLTGVTTWYQTIRLDSKAPLALSHGYLFASIEVPVSDHIFDIFGGSYFNPKNIYHQPMSNSRTIRVRDFQGKTLLSLPLKYDQNYNFAVTVDWIQNTLTVYSSIGTDPLKKAVGPMYNDPKAISPEFKHKGEYHLQLLKYPLPDAKIPAEKRLDVPHFGFQEPIKQEHVSFSNVFVTSGTQIEQPEFSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QN59</accession>
    <name>A0A5B0QN59_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QN59"/>
    <dbReference type="OrthoDB" id="10624118at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="128" mass="15133" checksum="497577F4558FCD44" modified="2019-11-13" version="1">MIQHDANLEDSNNNPNQESEEDQAHMERVWLRLEDLLHHRTDSLPERIIRTLDEELDELEARVPVNRTPEDHGQVANWETILEDELESIESDEDNHMGVEPPPIIRVDKRSDRVNTCKWYPFKSKLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNB4</accession>
    <name>A0A5B0QNB4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNB4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="60" mass="6858" checksum="2E347F7F44551925" modified="2019-11-13" version="1">MALSVEFSRCAGVQGNCTELNNLLPSRRQTEYPKTKFDEQIYGYGFLKLSFFRRLAASGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP04</accession>
    <name>A0A5B0QP04_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_030017</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP04"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001278">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="314"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1115036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="38975" checksum="C12EFABFC68CB0D9" modified="2019-11-13" version="1">MLKLPSSAFLRRPRLQPGLLGRTSRGSRDLQTHTFCSSGPSRSHDNPLGLPAQQAGLPRSGSPPKLNISRSLPTKRDIPNVRKVLVVASGKGGVGKSTVAVNLAIAIRNQSALNVGLLDLDIFGPSVPKLMGLDEGLSPELTDQNALVPLRNHGISCMSIGFLIPPSESPDSVVAWRGMMVMKAVQQLLFDVDWRGRAGEEEGPGLDILVIDMPPGTGDVALSLGQLVNIHGAVIVTTPQDIALIDVTKGVNMFRKLNIPIIGSVLNMSSFKCTKCETKHEIFGPMTSFKRVLERNNVELLGQVPLDLQISKSSDAGQPISISKRPPPEHPEPPDANTPTSASFDDSPFIEISRKCLDHLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQ07</accession>
    <name>A0A5B0QQ07_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_028960</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQ07"/>
    <dbReference type="OrthoDB" id="3196451at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF25">
        <property type="entry name" value="F-BAR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="181"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="57"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="533"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="553"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1114995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="604" mass="67510" checksum="F462CC00789CC676" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQE0</accession>
    <name>A0A5B0QQE0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_037023</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQE0"/>
    <dbReference type="OrthoDB" id="2510378at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022885045" evidence="2">
        <location>
            <begin position="24"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="268" mass="29241" checksum="8AEE0C68CD13740B" modified="2019-11-13" version="1">MRTPEACFFFLFSAVQLFDTSVGIFNCFRSRPNLSGMNEPEEFFLLSAPVRLSLRNQQTIYANDRPVVLVGASEGYWTIKGLREGYGCVVTRCPSQETPRPHPNQGAPSDANLRIPSYINQPTHSSWSLRPFSHFHEGSSSSSSHYSTQATPSHSNQGSPQHPEGTPSDLNEETPPHANQGSPQYSNPGSPPNQGSPPQSHQVLPSTSSQASPPHSSQVSSSHSSDEASHEDTPLVFMQGLEKDVEHILQAKTTTKLELNLYILTGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QRA3</accession>
    <name>A0A5B0QRA3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SPT3 Dosage dependent suppressor of Ty-induced promoter mutations-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SPT23_2</name>
        <name evidence="3" type="ORF">PGT21_036279</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="71307at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057962">
        <property type="entry name" value="SPT23_MGA2_DBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25603">
        <property type="entry name" value="SPT23_MGA2_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IPT/TIG" evidence="2">
        <location>
            <begin position="1392"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="793"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="894"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="982"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1133"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1291"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1354"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="588"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="982"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1133"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="1181"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="1201"/>
            <end position="1214"/>
        </location>
    </feature>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1312"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1363"/>
            <end position="1384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1542" mass="166652" checksum="755803F087FBBD7F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTM9</accession>
    <name>A0A5B0QTM9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTM9"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="11273" checksum="017E520ACC8902F3" modified="2019-11-13" version="1">MQTNATKLWCIASHCFADAILIHCIEESDASNAMRGPASLLRCDAIKARYDAIPMHASHQFHQPRRSSPAHLRTTLSTFLILQSTITNQNLEDPVLLTSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QTY2</accession>
    <name>A0A5B0QTY2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003779</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QTY2"/>
    <dbReference type="OrthoDB" id="2338662at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022726846" evidence="2">
        <location>
            <begin position="31"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="638" mass="70709" checksum="35D1A3D4CFD78149" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWR5</accession>
    <name>A0A5B0QWR5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SGS1_72</name>
        <name evidence="10" type="ORF">PGT21_022273</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWR5"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="51"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="511"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="644"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="662"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1117751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="696" mass="78366" checksum="065EFECF50BCD0EC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QWV8</accession>
    <name>A0A5B0QWV8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_023205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QWV8"/>
    <dbReference type="OrthoDB" id="10424493at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="54" mass="5966" checksum="5BE39DC962A7067E" modified="2019-11-13" version="1">MDAYATTILDTQESVLQQNIHPQVGCSSLPANGIDFFLLCKLRDGEIGFWPVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXE3</accession>
    <name>A0A5B0QXE3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein Asterix</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_004921</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXE3"/>
    <dbReference type="OrthoDB" id="2503145at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005351">
        <property type="entry name" value="ASTER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03669">
        <property type="entry name" value="ASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1117364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14935" checksum="1EF6CAC25F1460C0" modified="2019-11-13" version="1">MDRHLQQQHHRRRIKVSCLQLISSIQIDPFKRLKIPHFTPVTTIKPFELPSQEYEEFDPSAVLALACAGVSMVLQQHILAWLSLFFSVTSIVNNTNRTGRPSSMSPLQGLMFSVLALTTLYTKRLIDPTLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYG1</accession>
    <name>A0A5B0QYG1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_032309</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYG1"/>
    <dbReference type="OMA" id="MEPGHRI"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000188">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="270"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="312"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1118139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="355" mass="39489" checksum="C2E9ECBC2F9A8270" modified="2019-11-13" version="1">MNISSENSTNLRYSWEEPIESNPQFGSNLCATSPSYGLDYAVPHDVYLPNTQPAHVADLGGLQNNIRPYDTNWGFFENYNTPANFDCDGTNDIGEPAHRIRMNSMASSASSQSYSLTDDKRIYLPSIQSEAPDILLGCNTPYRTGSQSSYDTTYSSHYDDRITPQSHSIHPDQPHQGGVGLPTSCSFGGYPEIYMQSFRKDFRAKAPTYSLDRPYKCDKCGSSFSRNHDLKRHARIHLDIKPFPCSCCDKAFARKDALKRHLLVKGCSKTKEGVSRGSPQNSERQKLSKSPNALGPKYAGQQDKSLRPSLVHSRSNVNGNQSSPIHVPKSTGTGIPSQFNEIKLQNIKCSMPNFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R016</accession>
    <name>A0A5B0R016_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Concentrative nucleoside transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_003408</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R016"/>
    <dbReference type="OrthoDB" id="6075923at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008276">
        <property type="entry name" value="C_nuclsd_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011657">
        <property type="entry name" value="CNT_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002668">
        <property type="entry name" value="CNT_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10590">
        <property type="entry name" value="SODIUM/NUCLEOSIDE COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10590:SF4">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 28 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07662">
        <property type="entry name" value="Nucleos_tra2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01773">
        <property type="entry name" value="Nucleos_tra2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="491"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="549"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="588"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Concentrative nucleoside transporter N-terminal" evidence="9">
        <location>
            <begin position="207"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside transporter/FeoB GTPase Gate" evidence="11">
        <location>
            <begin position="289"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Concentrative nucleoside transporter C-terminal" evidence="10">
        <location>
            <begin position="391"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1118719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="609" mass="67700" checksum="323E9842C721B712" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0I6</accession>
    <name>A0A5B0R0I6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0I6"/>
    <dbReference type="OrthoDB" id="10578737at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022986192" description="RRM domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="65108" checksum="F24D915BA0B1EFD4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R207</accession>
    <name>A0A5B0R207_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029940</name>
        <name evidence="2" type="ORF">PGTUg99_035496</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R207"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366606" description="TNFR-Cys domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1119602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11878" checksum="8AAC162B0EA9C6BD" modified="2019-11-13" version="1">MRLTHKKCLGKTLILLAVNFMPLTSTGICPKDCETLAKFPNILDQCGERYSCNNCNLYERNKRCDTFLSGEVHQCPKHCPNQTFFASQNCASHHFETCNEHSHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2N2</accession>
    <name>A0A5B0R2N2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2N2"/>
    <dbReference type="OrthoDB" id="2851338at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1119776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="194" mass="22068" checksum="E9DDFE4C3B337478" modified="2019-11-13" version="1">MSRFPEFETGARYVAIDGQAPGFAAIYQVSSLGLFGQPRYQSLRANRSAREAELFTRIGTIDRRTYRCTLQVQRKTSSSDHQTYHSHILTVELAEPSPELLSEIHLLDGWRKTHVGETVDSSIVGRLQSASSDPFRTPRFILIFEFANLDFQRSEQLARLANNPEANQTVIRKWMLYRGWKNSCNMNPGSAQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2U8</accession>
    <name>A0A5B0R2U8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-hydroxybutyryl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_036481</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2U8"/>
    <dbReference type="OrthoDB" id="5958943at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022694">
        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000105">
        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="7">
        <location>
            <begin position="38"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="6">
        <location>
            <begin position="234"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="4">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1119951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="35888" checksum="56CA6AE32B4CCF57" modified="2019-11-13" version="1">MRLNVLLRNSGRVQSSRPVMRITPLRDLVLGSVRKISTVGVIGAGQMGTGIAFVAAKNAGVDVLLHDQLPAAQKKGLAFIDKLLAKDVAKEKMSEQEAKGVRDRVRAIGDLNEMGQVDLVIEAVAENLPLKQTLFSQLASICKPSAILASNTSSISLTKIAASAISPNHNPQQVTHPDPSPSRVVGLHFFNPVPVMSLVELIAALQTSHDTLDRAREFAVRCGKVVTVSKDTPGFISNRVLMPMINEAIICLEEGVATREDIDQTMKRGTNVPMGPLTLADFIGLDTCLSIMQVLYNQTSDSKYRPSVLLSRMVDAGWLGKKVGKGFYEYDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2Z0</accession>
    <name>A0A5B0R2Z0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_029410</name>
        <name evidence="4" type="ORF">PGT21_036217</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2Z0"/>
    <dbReference type="OrthoDB" id="3265969at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09276">
        <property type="entry name" value="Rnase_HI_RT_non_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="73"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="351" mass="40278" checksum="EDB0058CF3FC2474" modified="2019-11-13" version="1">MLVARSVQASDCYQFWQKTFKEKVFELKHNHWRKFLAESKDHQIFKAYKFTKPVSSGNIAPLLNENNELTSDKGEQARLLFKGSASAALLNNSISFACRINDADKASAFEAEVQAINIGLNIIKNEFQRNTLPSSNIINIFSNNQATLQVIAKPPRSTSNQAVFIQIFDKLNYLISVHQASISLLWFPAHVGIPENEKVDQLAKEATKGNRQFDLEQQPRSLSNIQQIIRSNFSFNKRKLPITRNQISLSNIPIKIFKSLNHLERDYQNNDGRYRCHLCPQATGTKNWRRHCTSVTHKRNVQRRDEARRLSQPVVNESVTHQGAPRPAEVNDHHNQEDHIGQTDRKLFANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RB53</accession>
    <name>A0A5B0RB53_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005758</name>
        <name evidence="4" type="ORF">PGTUg99_012629</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000214">
        <property type="protein sequence ID" value="KAA1122907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RB53"/>
    <dbReference type="OrthoDB" id="414243at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03192">
        <property type="entry name" value="GST_C_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03039">
        <property type="entry name" value="GST_N_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000055">
        <property type="entry name" value="Glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000608">
        <property type="entry name" value="Glutathione S-Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF252">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01205">
        <property type="entry name" value="AMPS.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00363">
        <property type="entry name" value="AMPS_(cytGST):_Alpha-__Mu-__Pi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="83"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1122907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="23518" checksum="D657CBD7637FF8C7" modified="2019-11-13" version="1">MPSYKLTYFGFKGGRGESIRMALHCGGIPFTDERLTHEQFAKIKESLPFGQVPILTIDEKTVISQEAAILRYIGRKTGLYPEDPEEAVKVDVLMCFVDDLYSAVPPFFAPEHPGKEVIIKTTIEERIPKMLNYLDKHLSDSLNNKGMAFSAGSRLTVADLRIYAALTLYKSGMLSGLPTDLVESSYPNISKLYQAIENHEKVAQWKKAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RI99</accession>
    <name>A0A5B0RI99_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020238</name>
        <name evidence="3" type="ORF">PGTUg99_020986</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000177">
        <property type="protein sequence ID" value="KAA1125576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RI99"/>
    <dbReference type="OrthoDB" id="2496723at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="780" mass="88863" checksum="19D629388CE6F629" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RT24</accession>
    <name>A0A5B0RT24_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001738</name>
        <name evidence="2" type="ORF">PGTUg99_011490</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000145">
        <property type="protein sequence ID" value="KAA1128023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RT24"/>
    <dbReference type="OrthoDB" id="107110at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1085283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1128023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="783" mass="87845" checksum="437E1663619E5E57" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RV37</accession>
    <name>A0A5B0RV37_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_014461</name>
        <name evidence="7" type="ORF">PGTUg99_027905</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000140">
        <property type="protein sequence ID" value="KAA1128713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RV37"/>
    <dbReference type="OMA" id="FTWFRYA"/>
    <dbReference type="OrthoDB" id="2499330at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF282">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138196" evidence="5">
        <location>
            <begin position="24"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1110214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1128713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="338" mass="37096" checksum="060E58A04689F344" modified="2019-11-13" version="1">MDAAVFLGLLHALFDGFLRFICANIPPFRRRWSVKEMPELSGRVAIVTGGNTGIGFATCLELARRGAKVYLASRTESRAKAAIQKIKQLVPDAQVDFIPFDLRILSSAKTAAEEFKGKESRLDILINNAGIMAAPYELSPDGIEVQACNATGHFALTSHLLPILKRTASSISDSHVRIVNVASVAHLFGTDPDFSSLEGMNRPNDWPMSRYDYSKLSNVLFTNELQKRLSDTSIYCLSVHPGLVASQITRSVISESLTDFLAKIGSYIISSPHEGALTQLYAATSPEIESKGLRAAYLVPYGQVGRKSLIAEDRGGKLGRQFWDLCERLMQAAEDKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RYF2</accession>
    <name>A0A5B0RYF2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017454</name>
        <name evidence="3" type="ORF">PGTUg99_005995</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000108">
        <property type="protein sequence ID" value="KAA1130482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RYF2"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF82">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="354" mass="37176" checksum="D48B48C864D5C65A" modified="2019-11-13" version="1">MWTSLVKSAFGLTMAAQMATSRTIVSRQEQCESATSDAAQAACAMKAAFGGSSIIPQLFPDFSPKAALSVKYGDVTIGGAQQMSPAKVAAQPFLSLSFISSDAQSFKKAYVVVGLEYRQKSKTMELMWLQSAVKIDGNTGLMSSSTPPIVKYKSPNPNPGSGTNEYILFVFQDPGLDNLLKQNPQVASALSGAFDFQGFMAKTKLTNQVVAGSYFKSSVDGSFINSPAPSASTPSQAQVPQTPPVSSRVANDSSVPTTPPKSQYQAQSQPDSPTAPIAPVQSMSEAAPIAPVQSTGEAAPITPVQSISEPDTPSAPSNQTEPEQKVTSNSIITLEAQKYMLSLIAVVVFGVSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LHD9</accession>
    <name>A0A5B0LHD9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">APE2_1</name>
        <name evidence="16" type="ORF">PGT21_028169</name>
        <name evidence="15" type="ORF">PGTUg99_003029</name>
    </gene>
    <organism evidence="15 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="15 18">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="VDEP01000526">
        <property type="protein sequence ID" value="KAA1063761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LHD9"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000014">
        <property type="entry name" value="Aminopeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11 15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="15"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="272"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="567"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1063761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1106065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="910" mass="101520" checksum="7BC08D1A276A63A9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LL56</accession>
    <name>A0A5B0LL56_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004995</name>
        <name evidence="2" type="ORF">PGTUg99_022987</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1080029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LL56"/>
    <dbReference type="OrthoDB" id="2500177at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1065617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="381" mass="43188" checksum="FB76C8884538BE0A" modified="2019-11-13" version="1">MRLKTPNTLSICFFVLQLIFTSRSWLKGFKLKGIGASGSAQKQVTFASEATGSASQSAALVKDDGYWFPLPEKDSMNSLKVYKDSIGTAPMENPSSNQIPFLRANNPEPAQDTEQLTNYYRTVSLEPSSWESTDPRKAFLEGMGKIAIQDQYHTSLIQLVFKRIWEKPDMTEGEVKQWLEVLKYYLKKDITTRDPTAKQIPVSVLYGLHSLQLKYHQNGQLLPHIMATSAVVQGYLDKIKPTVNIKDLENVPWQKEMLFPSQGFSRQLGDMKSYQKLIPNDQDFKNAMDPNVRTSLQGLTALKEKLSSFSAHKRVLVLATFFQVLKANPHNAMAANLLREIHQSSPFQHEKELITFILQHKPNKSQSRIPSTSSSSYQRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRW2</accession>
    <name>A0A5B0LRW2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_015530</name>
        <name evidence="2" type="ORF">PGTUg99_025566</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRW2"/>
    <dbReference type="OrthoDB" id="2496720at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="49440" checksum="D88CBEA8617C3181" modified="2019-11-13" version="1">MKTIMDLPDEVLERIFTNLVTINLTSSMWLPIAIYEHVKQQEVAKRIRLISEMRLVCRKWAEWFYQRHLYDQLSFKCSSRATAFINELARRPKTLPPPQCRYLKVQELWTWGLVLVAPPGKAEFKNLDALIDQFSETIVALEIQAVNLFTLPISTIERIGRIPNLRSLRLGIHLTRVGGGKTKSGSSVHRDLMIDGLPTDSECLVSLLRAAQGVVSLDLTNFRPICSPSSLGEDLHGHQFTSITTLKLDVKNEGDPTLDGIVRLATRLPNLKVLWIGGPGHMGDDLIPLFEVLRERLEELFVTDARVIRHALNLNFPRLRVIRVHEWDREFEQFLRQPMFASVEILALNCYPFHKRKKKASLWVPFETLPHLRRLELHEASQFPPPRSIEARCEDHGVECVYARHDIFEEDGRPNLDFDAPSALMNQLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVA8</accession>
    <name>A0A5B0LVA8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Phosphatidylethanolamine N-methyltransferase</fullName>
            <shortName evidence="13">PE methyltransferase</shortName>
            <shortName evidence="13 14">PEAMT</shortName>
            <shortName evidence="13">PEMT</shortName>
            <ecNumber evidence="13 14">2.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CHO2_2</name>
        <name evidence="16" type="ORF">PGT21_004665</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Catalyzes the first step of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.1.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13 14">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13 14">Endoplasmic reticulum membrane</location>
            <topology evidence="13 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the class VI-like SAM-binding methyltransferase superfamily. CHO2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13 14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.17" evidence="13 14"/>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVA8"/>
    <dbReference type="OMA" id="RIWYSVG"/>
    <dbReference type="OrthoDB" id="4583at2759"/>
    <dbReference type="UniPathway" id="UPA00753"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03217">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016219">
        <property type="entry name" value="Phosphatid-EA_MeTrfase_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138:SF0">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51598">
        <property type="entry name" value="SAM_CHO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 13 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 13 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 13 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11 13 14" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12 13 14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 13 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="592"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="667"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="353"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1018"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="393"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1068877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1107" mass="127900" checksum="C1B3D346096D1119" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVC8</accession>
    <name>A0A5B0LVC8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027593</name>
        <name evidence="2" type="ORF">PGTUg99_050228</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVC8"/>
    <dbReference type="OrthoDB" id="248233at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="373" mass="41107" checksum="AE75B98122F16355" modified="2019-11-13" version="1">MFGEEDSESPRRPSPWLPSPGKSSLSVCATPDDGPSQAEPKLPNWPDELEPELDELGHIEYKLKILSPTPSRFEKLKTQLKWRLLEGGGTALYELGVLDDGTLVGLSRTEMDESLANLARMARELDCELELLRVVEIPEIIVNTGPSSRPATKLPPSLRFPGGTDRKEKAKQKKKKKKSRAKPAMGLNSGIARPPTGKLRIEPGDAVVSSPSEPSSPREQPAPQEKPAIVVTSPRHSSRGSAHSLRTLLDGLHLHPRRPPANSNFIDQICLLTPEERSVIRRARRNERRAAQAAETRRSKARAEPAEATSESNADDHKTRFPDLLDQDFGGWKRGAEDQGGIKYVVEVCVAKEATAQEERFLDFEGFGISDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LWF4</accession>
    <name>A0A5B0LWF4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001943</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LWF4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="92" mass="10668" checksum="172C528F548A05AD" modified="2019-11-13" version="1">MLPRRRPLPNKQETFIYNADNPVLGHSDVQGTTIRLRKPLSNSGGMPIRRTHTRHRQYCRLSSDTLDDTYVIKVCESAHYKGREIPNPLLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LX25</accession>
    <name>A0A5B0LX25_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_023565</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LX25"/>
    <dbReference type="OrthoDB" id="10264376at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000245">
        <property type="entry name" value="WD repeat-containing protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051858">
        <property type="entry name" value="WD_repeat_GAD-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017">
        <property type="entry name" value="GASTRULATION DEFECTIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="87"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="188"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="238"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="285"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="458"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="549"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="561"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1069407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="583" mass="64024" checksum="AC5451EAE1A4BE59" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYW0</accession>
    <name>A0A5B0LYW0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Golgi to ER traffic-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GET3_22</name>
        <name evidence="2" type="ORF">PGT21_000373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYW0"/>
    <dbReference type="OrthoDB" id="2509730at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="370"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="818" mass="87624" checksum="13E55CAE4A5EC892" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2X6</accession>
    <name>A0A5B0M2X6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_026061</name>
        <name evidence="3" type="ORF">PGTUg99_015104</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000078">
        <property type="protein sequence ID" value="KAA1132615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2X6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366077" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9497" checksum="2906C14B3E60BEE8" modified="2019-11-13" version="1">MKCHLLCGVLLCRLDFRALGRPGFRLGIDQLQPWIEGALNVGSSGNRKHPLENGRPSSSFIHRKLREKTQGRTSWVRWIMPDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5D3</accession>
    <name>A0A5B0M5D3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_026492</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5D3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022744494" evidence="3">
        <location>
            <begin position="18"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1072035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="257" mass="28572" checksum="62305CDE417479CA" modified="2019-11-13" version="1">MAHWLILLAVFGHCTLRMRCGKELSALHLEKADQGADLRSHLGQRPPSDSVPANPANQPIPLQPDADALNHALSAFGPHSNQVQQEANIVLPSSDNFLEDSTAHLHVSQEDSRAMTALEADPAADQVQAANNHHFVIEQDGTRQSLTERVRTLGRELSRFGVDFGRTLWRNPPPQTLLGAAFAYIMCHPETSGRTLTNLGKQSARSRNGMGTNINSLQLTISDVCSQDSPVVVVLLHILFLVWLYYFIKDFNRYRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6N4</accession>
    <name>A0A5B0M6N4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6N4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="123" mass="13611" checksum="309DACEAC19BD379" modified="2019-11-13" version="1">MCGSFHYGQLGVAARRSQRQQALYDHCQSRRSKFPFLANQRPSAGSPSSSSTLNPSSAISIADSPSYTHSPRFTNNGPPSSHDPFIDIFSMDLAASELPSSSIQYKIRLVNSINSYRYSLRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7D7</accession>
    <name>A0A5B0M7D7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-galactosidase</fullName>
            <ecNumber evidence="3">3.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_023672</name>
        <name evidence="8" type="ORF">PGTUg99_019932</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="8 11">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7D7"/>
    <dbReference type="OrthoDB" id="5795902at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000097">
        <property type="entry name" value="Alpha-galactosidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF75">
        <property type="entry name" value="ALPHA-GALACTOSIDASE MEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Alpha galactosidase C-terminal" evidence="7">
        <location>
            <begin position="331"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1066746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1071948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="444" mass="51039" checksum="42DD7FC5D6B2F22F" modified="2019-11-13" version="1">MNTDYTADQIIQCPIIHHPSGRKRRTNCQRPMANAFNAGYPLNYSHPGSQMYINSVVDGLYSWNVSFVKLDALVPGSSFDPADYTKCDTRADLAAWRRAIDSRYEEEWKHVGRERIWLVASWAIPTIEGPTMDLNADSWRVEQDIEAYGERMTTFDRVIRNIKTAALWTSVEKNRAWRGLIDLDSLLVSDMTYEESKSTVTLWAVLGSPFYLGDDLTKLPESRKALVKNVEVLEVARLASWNPARLERFNQTRLEIARTKWQSGTQVNIEDCKKLVEKRRMVVGLSLHQTVDPILDLNRCLESQISHSRSLPISHTPASSHLDTPERSEWSLQFWVLEHQDGVLFLAIVNAGAQNMFDLPVEVEIKLSELERFQGTWSPSKENEEERWYLVRDLWKRETLGMVSFEGKMKLKLDVHGSVLFRFTPIGSDDHSQHHHPLVSEVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7F8</accession>
    <name>A0A5B0M7F8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028197</name>
        <name evidence="3" type="ORF">PGTUg99_018899</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000040">
        <property type="protein sequence ID" value="KAA1135227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7F8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="123" mass="14197" checksum="4288E434CD16C58F" modified="2019-11-13" version="1">MYIKTPFYWFLLFVANSIFQLTLQHQLIIDGRMIIPNDIITLEGKIDGKSQDLVAFKNCKCKESDGIHWSMKNESDETLLCVVKRKNNIIFSQHIGGGTSARSFHLNIPFNEPLYVDVYLTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7V5</accession>
    <name>A0A5B0M7V5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034367</name>
        <name evidence="2" type="ORF">PGTUg99_011333</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000245">
        <property type="protein sequence ID" value="KAA1120308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7V5"/>
    <dbReference type="OrthoDB" id="2510371at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="210" mass="23547" checksum="707A9408BACB96E0" modified="2019-11-13" version="1">MDISISSSSRRGICLYPCATGLWLPLVLPLKVHEKLQLCFSSPKFKLSIKLIVQSNSTSATCLDSLDLIENYLLNHPTQIKMSKSSQDNTFKNQTPQAEMTLKNAGVPLETEPEAPAPDIQPATTTQPTPLEEVEYIYDVVSAMKHALIQDHNSELFLETPNFNKVHEEYIKALEVFLERVSAIIKETLEAGNRFIYDSLDIPQISSPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9M9</accession>
    <name>A0A5B0M9M9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9M9"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042862">
        <property type="entry name" value="RNF32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14991">
        <property type="entry name" value="RING FINGER PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14991:SF0">
        <property type="entry name" value="RING FINGER PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="135"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="197" mass="21791" checksum="866BF0F2148A1F02" modified="2019-11-13" version="1">MSSPPLRIIDPEPGRAGEPGSAIRCGETGQDPMASLDAVASAVADARRALQELHHETSTLQLISAQLHRHALLLRLIHNAMALSLQPALEPPHPSRQSTHQRLDLVDSSGNPIHTLASVEAFKSNPEHNQQIPHCLICLEDYILDSPIVVLPCHHSHNFHLTCLTHWARYCQQLLCPLCRSPYEQERSSDDDLPAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MC65</accession>
    <name>A0A5B0MC65_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MC65"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="290" mass="33115" checksum="938FE880A1A06ADB" modified="2019-11-13" version="1">MIQTLNELFPKQIPKPADEMNDQKFKEFKIYRLYHLNASIRTSLDKHLKSLFIEFRNILSDFILHEPVTFPRLVRDTGLEINQSIESAIGFSKASELSFIWTLWKNRICEFNQILEELLNQMGPMRLLHGENEAPLTPPAVKLGTSLIPVFKLSRLFFNKLSRERVMKEEVGMSTEMSSGQWSSLDQSIEDILDSVFFLKSLIGDADGVNPVYTSSAIIEELKHLTGLFHSLRSLINLHILPNLFPNSLDLSSRVYFEDWFDSWTTSLSNATHNAIQAAKSFQSSATPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MD35</accession>
    <name>A0A5B0MD35_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013754</name>
        <name evidence="2" type="ORF">PGTUg99_016225</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000474">
        <property type="protein sequence ID" value="KAA1073880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MD35"/>
    <dbReference type="OrthoDB" id="10287209at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1116434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="63" mass="6632" checksum="DDD799E40E0F1B7C" modified="2019-11-13" version="1">MLAPPRDREPAPSSAGSSSSLCGWRYVICSEGPPGALKFSGAMDRYEVVKASDQGDRSPKMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGG1</accession>
    <name>A0A5B0MGG1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SM-like, degradation of cytoplasmic mRNAs and positively regulates transcription initiation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LSM1_1</name>
        <name evidence="5" type="ORF">PGT21_032410</name>
        <name evidence="4" type="ORF">PGTUg99_033577</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGG1"/>
    <dbReference type="OrthoDB" id="10263346at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1076025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1091380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="155" mass="17993" checksum="BCA0A404F4B2AB3D" modified="2019-11-13" version="1">MDNFLTNVPFTTSGALVDIVDTNLVLQDTIERIHVGIESKEDQQDSNSQHDQQQIAEGSETQNNKKKKKKDGICKYTDIWRGIYLVRGENVVLLGEIDLDKEDEIIKRFEYHSLETVSELQQQEIQTKAERVKKDEKILFDRLGFSKEGDEDDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJN7</accession>
    <name>A0A5B0MJN7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hsp90 chaperone protein kinase-targeting subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CDC37_2</name>
        <name evidence="10" type="ORF">PGT21_022196</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="440202at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.610:FF:000002">
        <property type="entry name" value="Hsp90 co-chaperone Cdc37, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.610">
        <property type="entry name" value="Cdc37, Hsp90 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004918">
        <property type="entry name" value="Cdc37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013873">
        <property type="entry name" value="Cdc37_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013874">
        <property type="entry name" value="Cdc37_Hsp90-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038189">
        <property type="entry name" value="Cdc37_Hsp90-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013855">
        <property type="entry name" value="Cdc37_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800">
        <property type="entry name" value="CDC37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800:SF4">
        <property type="entry name" value="HSP90 CO-CHAPERONE CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08564">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08565">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03234">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01069">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01070">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01071">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101391">
        <property type="entry name" value="Hsp90 co-chaperone CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 N-terminal" evidence="9">
        <location>
            <begin position="4"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 Hsp90 binding" evidence="8">
        <location>
            <begin position="178"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 C-terminal" evidence="7">
        <location>
            <begin position="380"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="181"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="472"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="482"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1076852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="516" mass="57089" checksum="818415AC7BCE2F55" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNL6</accession>
    <name>A0A5B0MNL6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024683</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNL6"/>
    <dbReference type="OrthoDB" id="2238745at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="660"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="945" mass="103430" checksum="EE08F75812D14304" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MP58</accession>
    <name>A0A5B0MP58_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031881</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MP58"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="153" mass="16858" checksum="BFBF09B694AE4920" modified="2019-11-13" version="1">MYLVDWKEILPVNEVHVPRRLEGSPSSQRGTCTSLTGRASFRSTRYMYLVDWKEALPVSEVRVPRRLEGNPSSRPSPLSGLGYPWGYPDIRRDFTEKGASAPESAPASGYPLALAGIRQRIADIRDGLSPPISIARIWAPLAFPVKLAGICPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPC5</accession>
    <name>A0A5B0MPC5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPC5"/>
    <dbReference type="OrthoDB" id="2497493at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022941892" evidence="2">
        <location>
            <begin position="20"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1078088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="292" mass="32256" checksum="FC9C377428A8360A" modified="2019-11-13" version="1">MHARQLVTILSICLSQAHARSIPTVSDPLEPIVRLVTTPHEVLGAAPDLKETEKAAIQQSPYLYSHQIAAGLAETTDSLHYLSSEPQTAFSSLMAGSLLGYYRTIMQDLPKETKESRLQIYRNSFYSRTRDVANHMGPELWNTHATLAKKSLENGLQDVVLEFQKSQMAPAGDEKILKRIEILAQKFKDNKKHQEIMEEISKAVAHMKTHLPYNVNLGEMKYLSTEEVESALKGTNIGGHPDAIPVNYFLVHHEPATHQIAPAKTPITNSRQAQLPPSQPKVATAPTAAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MR30</accession>
    <name>A0A5B0MR30_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pre-mRNA-splicing factor cwc22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">CWC22_3</name>
        <name evidence="1" type="ORF">PGT21_013726</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MR30"/>
    <dbReference type="OrthoDB" id="3047342at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="84" mass="9578" checksum="1A338929CF601A62" modified="2019-11-13" version="1">MNEDDTTSSSRIFVKIMFKDISEVLGLKSLAERFKDPSMQVWYGGLFPIDNPRNTRFSTTIVVTSIGLGVLTEEMREHLKRAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRF9</accession>
    <name>A0A5B0MRF9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRF9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="126" mass="13924" checksum="EA32DF9C72DE889D" modified="2019-11-13" version="1">MAPRDVTSDDRWLEIWRNFSPRDLQANQDEGELTLACHKQVTSLKTVTFTNYAACAACMLRPAETLALAGCVICHKDIHLAAGIWRWRPALPASTTGQGICHRMCRSDSTDSHTLLEAQQPQAGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MU67</accession>
    <name>A0A5B0MU67_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_010531</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MU67"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="2">
        <location>
            <begin position="257"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="478" mass="52778" checksum="1EF2B7D9D261141B" modified="2019-11-13" version="1">MQARSFSHSRIYVSKTLLMQVILQILLILILPKANGHALQVYSGRQNPSPRHVLHARQELFQDGGVGYKLPLGAKPFQPYKCGRKPLSPKTWKELRIDEYIKTYPRGLVANLTVFAAENKAINFACGMNQFCSAGQLCSPISGRAWWVLAAVEQATMYFNSLSTAIGFAAGQAKAIGAELVNDLYLQDAKKKYRLFQSIAMVLTMALAVTAMIAAVVFMTVTGTVAFAVFATAGAMIAIAQVTMILKAQAEEVEAGNQDTFTKWSHYEGRISDWQSKSQTEIGKRVKDMVEYPISSHRGLFGLLEGGEYCRNNQKQDSNDLEKKMARILTIRFANQILRAKKGFITIGEGCYSKGPNGAFSLDEGWLSYCDKPGGLMYNVIYDDDGKSGNQFYNARLLVEKYHISVEYIVKQAVECSQLGKGPDYDPYADGTNLPADENARCVMNLPVCDTRGAGVRKMLKHHTTVKACRVAGGINLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUI9</accession>
    <name>A0A5B0MUI9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011440</name>
        <name evidence="3" type="ORF">PGTUg99_024696</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000103">
        <property type="protein sequence ID" value="KAA1131524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUI9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137312" evidence="1">
        <location>
            <begin position="21"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="114" mass="12807" checksum="6C9602CB1352ED73" modified="2019-11-13" version="1">MLPVLKIMISIMLTGLMVHTKQFACPKEGCSGYSLQSEIKRSKGTPAICNTMTICAHHDDCCRCQKKVPEQYHICALCQHRWTKPLSDECPRLKQGNDYHFNLECPRPDAAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUN9</accession>
    <name>A0A5B0MUN9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_012447</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUN9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1079470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19928" checksum="91C3F4E05662E21D" modified="2019-11-13" version="1">MNEALIEGTFAALYRLRRRLQLFFLDEFDGLYEIFEELRDNPHCNGLDDDRYQRFLGPVPSHIVRAFVRLDEGELSNEDAFTRDPLETPLIQIYALWRSLIRSLHTFRRATFAFLDSLIVSDATAAATSPDGEALECQICAEDIIQPGQIILQLPCHPTHLFHRECLQVSIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MX09</accession>
    <name>A0A5B0MX09_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017949</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MX09"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="77" mass="8290" checksum="52A6E0C0110B73D1" modified="2019-11-13" version="1">MEEVMEEVMEGLTGEVMGEAMVEAMEEAMAEATVEAMAEAMAEAMAEAMAEDMVEDMVEVMAEVMAQDMVEGMEALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N111</accession>
    <name>A0A5B0N111_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_020701</name>
        <name evidence="2" type="ORF">PGTUg99_029341</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000205">
        <property type="protein sequence ID" value="KAA1124135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N111"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1124135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="95" mass="10250" checksum="F637AF789FC2BC61" modified="2019-11-13" version="1">MCCAITRRTPTGDSSLVEPPSTVPLCSYTLVAPKVADNLTIIVTSAATSPITGTWRTVHGIPTNVLSTNRRELWLPPIETAYSGFSPDWLHVKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2Z9</accession>
    <name>A0A5B0N2Z9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2Z9"/>
    <dbReference type="OrthoDB" id="10277211at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1082946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="120" mass="13257" checksum="8C9B9F87B4B500D3" modified="2019-11-13" version="1">MITAYNSSPATAVTLRIPLSSACGVPLDRSPTGPYIWPADMFESLSTSFAISKNRGTRALRLYTSLNESIIFFTFNKIITDGRKDAVQHLRRPQLEVTILFATSARAIDPSTPICEYRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N544</accession>
    <name>A0A5B0N544_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N544"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1083604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="142" mass="15153" checksum="578A705AADB1C824" modified="2019-11-13" version="1">MLSPDSSIGRRATTLGQHTITILVDSIRICKSSHEVGGQLNLQLFLGGSKLVDFWEEQLDPIRSVVSNLLQTPTIGADLVAIHPISSSKAELCRIDADQLALAGGVVDARRPSQFKVFINLPPAACCLASLTLTPASHDDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NB21</accession>
    <name>A0A5B0NB21_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NB21"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1086525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="8808" checksum="77E849D0D0406497" modified="2019-11-13" version="1">MPYAFGDLIITETQKQLYASDPDQPNTCKFLLCPSPRRSQGPLLYSLPVGTKLWVQAEMIAVFGDIIKLEVFYVQLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDB1</accession>
    <name>A0A5B0NDB1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDB1"/>
    <dbReference type="OrthoDB" id="10294715at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="106" mass="11402" checksum="AACDF36FAD9829AC" modified="2019-11-13" version="1">MPENDKKCPKCLAEDTNEKPRSGRAPKYGRGLPTDSGPYGFPNPPPDRGGSLSPPSSVYSATTTNGKNVSTPWTTTNTINAIWVNESSGNGIPNFSNEPRFSIWTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NEC1</accession>
    <name>A0A5B0NEC1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-dependent RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_035256</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RdRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NEC1"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF55">
        <property type="entry name" value="RNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RDRP core" evidence="3">
        <location>
            <begin position="439"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1173"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1177"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1187"/>
            <end position="1197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1087671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1246" mass="140688" checksum="3CE7194B12FB10BF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NFS3</accession>
    <name>A0A5B0NFS3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NFS3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="323"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="429" mass="47412" checksum="18D84A2D8EA2C497" modified="2019-11-13" version="1">MFQPCHRCDRARSAISVPSPRYSSFLSVGTSLLRLVRSVTISLSLEFNRSPLNLDSNLVTRPTATTSRLCPRNSTDGGSISTLRMSLDLNTVTSPIWLQSCHRANFLLTENNGLSLLTGKPSAPAFLCSLSPRTPKKPSLLSALPPHATSIERYPQNLSHYACRHAFLLLASSVGRSDPSIDIGVRYYPDRDQWSPIFNPRVVPDDNADGNKGPIEAVGNKGPIEAVGNQGPVDIKPIVEAKEKGPAEATVDPASINTIDKWHAINDLSRHYDCVPIPRLFPKPHPFVCVPWSVHDPELPPGLPVPFLRNLNLPVPAPTPAPSTSSSESSSNDSDSSSTYPLISNYINLNHQGVQKLLLDDNTPPTAVCIYTEEDVMRIFEGIAPEYVDMCSEYPPSSLAESDRRVMFFENMQWFFRNKRSTYLSTRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NI70</accession>
    <name>A0A5B0NI70_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_033813</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NI70"/>
    <dbReference type="OrthoDB" id="332281at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009316">
        <property type="entry name" value="COG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024603">
        <property type="entry name" value="COG_complex_COG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024602">
        <property type="entry name" value="COG_su2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12022">
        <property type="entry name" value="COG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06148">
        <property type="entry name" value="COG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 2 N-terminal" evidence="10">
        <location>
            <begin position="44"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="COG complex component COG2 C-terminal" evidence="11">
        <location>
            <begin position="506"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="405"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="621"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="641"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1087569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="891" mass="98732" checksum="581E460B56F15F3A" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NIN8</accession>
    <name>A0A5B0NIN8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033909</name>
        <name evidence="4" type="ORF">PGT21_036130</name>
        <name evidence="3" type="ORF">PGTUg99_014078</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000440">
        <property type="protein sequence ID" value="KAA1081824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NIN8"/>
    <dbReference type="OMA" id="GHVMFRT"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000168">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000023">
        <property type="entry name" value="TBC domain-containing protein C1778.09"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="178"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1066575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1087729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="456" mass="51935" checksum="E67B6306B3CB58C8" modified="2019-11-13" version="1">MAASPSQHPSATIDLESAQHVHQVYTYLDKVGVPGDGFHEGIERTREHRRLSALASLTQLAAAAHSDSLSDAEIQILGNADRYGFFKPMSSERVLYLPSDAFYPTGVSTTSDPILATSPSALNSTNHDLLIQETNRIEKWTEMLTASSRDSGSSQSAYQLKPEWHQQQNRFEKRCFKGIPDRWRRAAWEMLLAETDHPRQSSTRPTIQRLHQQFNALLTTPSEQDVQIDLDVPRTINGHIFFHTRYGKGQRALFQVLHTFGMYCKTCGYCQGMGPIATTLLSYFSAEDAFVCMVRIHDNLQFHNIFSPGFPGLLECFYIQERLIEYLIPAVHATFEKQMISSSAYATKWYITIFANTVPYEAQLRLWDAFFLKGMDVLILAAVSLVYCLRSKIGPGHDFEEILSTLGGRFEMENSSRWLRSIRKLHNRHDIQDLIHESRSQWNSFVRDGSASKRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NKH4</accession>
    <name>A0A5B0NKH4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP synthase mitochondrial F1 complex assembly factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_028268</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NKH4"/>
    <dbReference type="OrthoDB" id="16535at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010591">
        <property type="entry name" value="ATP11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126:SF0">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126">
        <property type="entry name" value="CHAPERONE ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06644">
        <property type="entry name" value="ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1089727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="40622" checksum="E34EB3998324F279" modified="2019-11-13" version="1">MNHLSNSILLGLVNCPSLARPSIRSRGRQIRYLGTSDTSDRTQKLIQQKYAEKINEKLKEKGLRDLEELKKINQDKIQAERIQQRKLYDELIKKARQKLNPDTDRTTETRSPPIAKSKQYSSPIKPLDSIIDLNKIATQSPSEIRKLWTAYHLSKSDPPKLGAVIPCATYQEMLQAARKYPSFVVPLAKSSAVDSSEQAKPADVPYEMQYLQWDFVKQPAPEPRLPDFLSKTPHWTQTTPSTIPATIVMYTPLGEYKLRQTFAQPTLILTHYTDLAESHGIVLMRGDITPNSNGSPKITAIEAQMLVLRLQQFYHATPSANQDQSVHLKRRLLLESFHEQPDRFQLSELLQLVNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMR6</accession>
    <name>A0A5B0NMR6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007526</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMR6"/>
    <dbReference type="OrthoDB" id="10372373at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1090625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="171" mass="19728" checksum="1B748B4EBAA6A9CF" modified="2019-11-13" version="1">MLAHWGKVDIAKFTTQDLESLVQEWIDKGGVSSLVNYQDFRKGWEPIQSYLLRKAHIDSLEEIRTLFYRSFLTAVQERIRDQLIKDKTMITTLENRFKLPAFDVLKKAVDEVMKGQTALTFEDSRSSNPVSTLLFKQSNEIMNKMETDRRPKEAPVSAKAPATIDEISKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NN55</accession>
    <name>A0A5B0NN55_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NN55"/>
    <dbReference type="OrthoDB" id="17560at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630">
        <property type="entry name" value="DIENELACTONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630:SF44">
        <property type="entry name" value="PROTEIN AIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022738424" description="Dienelactone hydrolase domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Dienelactone hydrolase" evidence="2">
        <location>
            <begin position="68"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1089359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="277" mass="30789" checksum="B4B08F285CFBF88F" modified="2019-11-13" version="1">MIKLLSFLIVFFAVPIFGKEVDNHSRCLSGFIHLGKPSGRIQSINGVNVYVANPTSHDSPESQSSAEGQKAILVFPDVFGIDLINVQLITDKLATDLNTPAYLVDTFAGGDIQPNKLPVGFNLTEWQKNHRPEQVLPIIETVIKNLTAQGVERFAATGYCFGGRYVFLSSDRNWIHVGSTSHPSLVQVPDDFLELREKSKAPLLINSCETDSQFGAEAQKQSDEILGNGQYKPGYKRTYYPGAFHGFGVRANLSNPPEKRAFDDSYKQMVHWFDAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NS03</accession>
    <name>A0A5B0NS03_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NS03"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="97" mass="10368" checksum="F1457A8E9F37DC90" modified="2019-11-13" version="1">MAVGKENRVERSLVVVCMTVTSARVGQSARLGPESDLTMTSSFLHLRASQPHSVCILMVLACAARGGEGRRSANSVGSENTPQQPPRLQQTATPMFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUM4</accession>
    <name>A0A5B0NUM4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_004130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2503993at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902929">
        <property type="term" value="C:plasma membrane of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024982">
        <property type="entry name" value="Rax2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048266">
        <property type="entry name" value="Rax2-like_second"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048265">
        <property type="entry name" value="Rax2-like_third"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778:SF2">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12768">
        <property type="entry name" value="Rax2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20842">
        <property type="entry name" value="Rax2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20843">
        <property type="entry name" value="Rax2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022920655" description="SH3 domain-containing protein" evidence="5">
        <location>
            <begin position="31"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1276"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="1466"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="238"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1310"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1435"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="474"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1389"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1435"/>
            <end position="1451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1092472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1522" mass="161475" checksum="4A3D389EED3F6AEB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NXP8</accession>
    <name>A0A5B0NXP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NXP8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1094021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="64" mass="7431" checksum="5413ED7C9880824E" modified="2019-11-13" version="1">MYAMVLSESSTYHTQFSSNSVFTNNLTRKKRVERHHHHPRFAWNKLRLIGLNRRGTPVGFAGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZA4</accession>
    <name>A0A5B0NZA4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF125-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_018281</name>
        <name evidence="8" type="ORF">PGTUg99_011328</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZA4"/>
    <dbReference type="OrthoDB" id="73465at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1067831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1093420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="35449" checksum="BA3BCD85396BF6CC" modified="2019-11-13" version="1">MGALKEPIPTMEVDGYGQHTCTERMCCQEPGSDEERTLIDIDVVRNIIIGLSDGLTVPFGLTAGLSSLGSSRLVVVAGMAELISGAISMGVGGYLASEADRDQFRYRQRVIRKRVANSCSNAMDGRVQELLQPFGISQGLCGMVSNDLLKFEYLRTTSSHQAKLSSSWQKSALGSLIMTIFSRKTPDYDSISQEKSSDEETVQLTGFESESLGLGMTEFLLKFGEGVEEVSTFQMYLSAFTIGFSYFIGGLIPMAPYFFIEKANTALFWSIGVMVLTLLVFGSLKAYFTGARIGFIGYLKGSLSTIVVGGGAAAASYWVVKLLDVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P080</accession>
    <name>A0A5B0P080_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P080"/>
    <dbReference type="OrthoDB" id="2499945at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1094312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="664" mass="69941" checksum="A7F37DEA9BDA4D6D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2Y5</accession>
    <name>A0A5B0P2Y5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_035057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2Y5"/>
    <dbReference type="OrthoDB" id="2506814at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="207"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1095054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="363" mass="42017" checksum="C9B351CE76236A48" modified="2019-11-13" version="1">MTCGQHFLDYLKNIVGLYPVLFMYQEDHRLLKTIDFSRPSTSQDHRHLKTIDFSRPSTSQDHRLLKITKFSKAPKQIHHGPSVLDSLLRGERVQSSIAVSTSPNLPNNDSHDHSTPDFDPPDEPYHDSSPYDENPLSTWSSIPWDISMDTTGNNTTNSQLPTVNDEDLAALRHHDRRVEFQTRASNWEGVMNKIFSAYLWLKEKTDNWTTEASFKSYDSWFCKCQEHEVRSKQWIDLVDLAGQQRVKFEFCRCMPRSVTLLANGFLPSSPIKPTTGFSMRLLAYHDYAWNHSNVQMAPFAETQRKFSEDRSEVLWNCRQTKQERKSDTNHPGPNQTAEDGTYNLSRLLWAKHENRSSSTSFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P588</accession>
    <name>A0A5B0P588_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002283</name>
        <name evidence="4" type="ORF">PGTUg99_012462</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000011">
        <property type="protein sequence ID" value="KAA1137186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P588"/>
    <dbReference type="OrthoDB" id="2501813at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="208"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="407"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="445"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="657"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="764"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="667"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="681"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="851" mass="94741" checksum="9445D0241F3471F0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PAE1</accession>
    <name>A0A5B0PAE1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PAE1"/>
    <dbReference type="OrthoDB" id="10292311at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022723045" evidence="2">
        <location>
            <begin position="24"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1098557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="362" mass="40503" checksum="C94151BAABBB452F" modified="2019-11-13" version="1">MLPFNYSTHLLLAHLGLSIAGKALERPAYDPFHQILDWGDVPPNLNSGLLDCTQAPAEAQRRLKVPTLAIPTSSGTELAQTEPFRNGLSMDNVPNDIDLGASLPPLLPNGLKEFNSGTSNQQLLQNFNAYTSVAGEVLEKSPRYIEGAPSCTTSRDGSENHISYTPQSERNEAVRCHQTWRRYKKTADNTVDPISTPSLQVQSSEPFPAWFANSPNLSFDSLSKDSLPPDHTSIYDSSINEINEPSSFSHLESPSYKRLRFTDHFMDPKTPDYNSKEWDILLDYLPIESEPTAQMEFRENHSNIGTSSHVANQVNQRHNNFPLETGGNYGRMVDFQTEPQSIMVEQADINRYLRAISHPNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCR2</accession>
    <name>A0A5B0PCR2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MpV17 mitochondrial inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MPV17_1</name>
        <name evidence="7" type="ORF">PGT21_035237</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCR2"/>
    <dbReference type="OMA" id="AYMKTRH"/>
    <dbReference type="OrthoDB" id="430207at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1098440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="196" mass="22109" checksum="0D26F157EE8FA35E" modified="2019-11-13" version="1">MGRLGRGYLRLLQTYTLPTQMATAAVIFPIGDAISQHLIDQKPWKDHNYSRTLRSITYGTLAWAPIAYKWNKTLNRITYPTSKLKTVLCRVGIDMALFTSFATCYFFTCMGFLEGRTWHEIKARIERNYSTVVWTNIGIFGPAQIINMSLVPVYGRPPFLNLVSLGYNCFLATVNNNTPSVIPTVSAEKLVIANTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCX6</accession>
    <name>A0A5B0PCX6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036646</name>
        <name evidence="3" type="ORF">PGTUg99_027802</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCX6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366379" description="CCHC-type domain-containing protein" evidence="1">
        <location>
            <begin position="33"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8112" checksum="963A426C512DA73D" modified="2019-11-13" version="1">MRLTAAPLQQVAMRLNVPVVLLAGLLLSTSLAMHLPAQRDVVPSCDSGLHLCEIRGCGHCGGRDHKTSQCPELPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEW4</accession>
    <name>A0A5B0PEW4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA-processing protein FYV7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030326</name>
        <name evidence="3" type="ORF">PGTUg99_022589</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEW4"/>
    <dbReference type="OrthoDB" id="2503175at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41805">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41805:SF1">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN FYV7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1120680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24742" checksum="F2916A10C38C59BB" modified="2019-11-13" version="1">MTIPPKKPTNQQDPSWKRSKGFRIGKNKPGVSQSVYLGKAEKIKKTLIHKAKLKKQRAKELAKAGYDTTTSGPAPESPTDNHQEDQPSGRTDSRRTQPGPSDSRRPKKSHQERPKDSSHPSTTKSQNSSNQHDRHHPKSSARAQQNHHPSSSELKASLKESTTKTRDPQQSNHHLKTPNRPARAKIQHANGQPKLSHRVSKILSKLEHEKEEDQSRLSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHX4</accession>
    <name>A0A5B0PHX4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LNS2/PITP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_012399</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHX4"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000063">
        <property type="entry name" value="Nuclear elongation and deformation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="4">
        <location>
            <begin position="765"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="110"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="155"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="303"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="553"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="615"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="676"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="985"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1021"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="556"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="572"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="584"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="621"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="683"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="985"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1030"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1041"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1054"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1067"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1118"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1149"/>
            <end position="1159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1101235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1169" mass="128370" checksum="20966915F2E56CA4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJQ7</accession>
    <name>A0A5B0PJQ7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein-S-isoprenylcysteine O-methyltransferase</fullName>
            <ecNumber evidence="3 10">2.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_019522</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="2.1.1.100"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="3 10"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJQ7"/>
    <dbReference type="OrthoDB" id="422086at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025770">
        <property type="entry name" value="PPMT_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF9">
        <property type="entry name" value="PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51564">
        <property type="entry name" value="SAM_ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1101421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="293" mass="34158" checksum="ACEA72F3EA4F6DC5" modified="2019-11-13" version="1">MVAAVTEDLQAFRRHRPMWADGEQTPETPDPSPSPSSSPHHQHEHEQEERISWPLISFENTIQNVCCISFLLGLFFAANLLSLLFFFLYSPAKQALWPPLNIYAILLILFHLLEFLTTAIFNPSRVSVDSFLLNNGASYWYAQMMSITEHVLRERYLAKNGWEVRMMKDDRVRMCGLMVTMVGQGIRTMAMITAARSFNHRVSTDPKKQEDHRLVTHGVYRLLRHPSYFGFFWWSIGIQLFLANPCSLALFSFVLWSFFKARIQNEELHLIKFFGKAYVDYRSTTSTYIPFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PL44</accession>
    <name>A0A5B0PL44_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022373</name>
        <name evidence="2" type="ORF">PGTUg99_018825</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000338">
        <property type="protein sequence ID" value="KAA1102275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PL44"/>
    <dbReference type="OrthoDB" id="2497097at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="164" mass="18268" checksum="61F6088DA6C610DF" modified="2019-11-13" version="1">MRFPYGSITPHGHPLYFAFWSLATVSAANLDDLDVNRWDQGPSARKLFTSSPEYPISSWAPSSHESNVQSEVSNSGAHRLSPSPLTEQNLLERLGDSSVIGEPSKDYFELLADDQAGSRKRVKHSQGQQLSTRRTPIYYEFMPGSSHAHDGDAARKKMVFLSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMQ7</accession>
    <name>A0A5B0PMQ7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PMQ7"/>
    <dbReference type="OrthoDB" id="2499742at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="42"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="310" mass="35135" checksum="6DC63180881EA733" modified="2019-11-13" version="1">MASDTVQTFRLLKTGCNIVRDPQDPKSIIAIIEFTKFSDLTEADREELNFVSTFLRKTTQFISHVKSKQRAWGGKMWGIGWRKSSDEDQIAGRYIKAFEAVHAQAYHDLFSLSGRVGQIVGRNFKKLAEIPFGSNRDLMAEHGLPSLAALEYGEDLTESNCAPHLTFTTDGFFNPPHTDDEDVSKYAFVMFLPTHTKDGSLATDEDSYDITSGPFIFPDHKFGINFDHQFGVVKMIWQANKYKHCTMPSSHSSRFTRLGMSVQINLGLKNACVAYQQGKYNSVANYFGDHFFYLYRSCLHLSLVKFALLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PMW8</accession>
    <name>A0A5B0PMW8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1102995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PMW8"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1102995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="123" mass="13421" checksum="2D4DF2E9EBC67C7B" modified="2019-11-13" version="1">MCSTSLVSFTDRFPSEPPPSSLHSRQASSCSNSIYAGSDSRSLAPKDEQGETIARSSGSPNKLKSSTIPPHSRRPSQGLSPESQTKPKFNPRPDLAKSEARKDRPTSQTILKNTQISSRTYWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PNK2</accession>
    <name>A0A5B0PNK2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_036522</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PNK2"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000244">
        <property type="entry name" value="Nonsense-mediated mRNA decay protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="27"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="937"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1102168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="987" mass="111276" checksum="3578846BBB9E67FC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PT53</accession>
    <name>A0A5B0PT53_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002648</name>
        <name evidence="1" type="ORF">PGTUg99_013768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000442">
        <property type="protein sequence ID" value="KAA1080122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PT53"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="112" mass="11921" checksum="6B4A696E44D870A3" modified="2019-11-13" version="1">MFSPIGDLHIGGTCTPGGTQLGQVKHGCPSAVTPPTRPCKPGVLLAGPACMYHRADPGPSAEIFRWTPGHRRFTVGQRDHFCPMRLHPTAPDHQLDPYAVAGVAGLYRVASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PW95</accession>
    <name>A0A5B0PW95_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016183</name>
        <name evidence="3" type="ORF">PGTUg99_015428</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000311">
        <property type="protein sequence ID" value="KAA1105224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PW95"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366482" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9224" checksum="1EFB7E8415C88C70" modified="2019-11-13" version="1">MRSFVLDLILQPSLGPCSALLTIVCTCTLKSAHRSGPNHYERSTSTDINIFAHNCWTSWSPVEARVNDCSMGMQQQSIPEIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PWQ3</accession>
    <name>A0A5B0PWQ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002655</name>
        <name evidence="2" type="ORF">PGTUg99_014569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PWQ3"/>
    <dbReference type="OrthoDB" id="10276469at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366461" description="Secreted protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15824" checksum="0F5BD4DA16C50684" modified="2019-11-13" version="1">MFLKDWSLQRSYIICAVIALTLLPSVQLSESREHSLVRRGVTHNCDVQYVNTPSTPDAKCVGKDNVPYSCAKESCALDFEFKNCQKINPNNPNALMGNGKFLKPDSYTTGSHYIVALSGDTVRGCPFNDNRQRQSCDVCLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXQ8</accession>
    <name>A0A5B0PXQ8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Trafficking protein particle complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGT21_015276</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10256906at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055505">
        <property type="entry name" value="DUF7077"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045126">
        <property type="entry name" value="TRAPPC10/Trs130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022233">
        <property type="entry name" value="TRAPPC10/Trs130_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056913">
        <property type="entry name" value="TRAPPC10/Trs130_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251">
        <property type="entry name" value="EPILEPSY HOLOPROSENCEPHALY CANDIDATE 1/TMEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23274">
        <property type="entry name" value="DUF7077"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12584">
        <property type="entry name" value="TRAPPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23036">
        <property type="entry name" value="TRAPPC10_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="6">
        <location>
            <begin position="53"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7077" evidence="7">
        <location>
            <begin position="768"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 C-terminal" evidence="5">
        <location>
            <begin position="1129"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1225"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1232"/>
            <end position="1242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1105676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1296" mass="145546" checksum="B2B1DA16EF9D0EC5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZR6</accession>
    <name>A0A5B0PZR6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034886</name>
        <name evidence="3" type="ORF">PGTUg99_030013</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZR6"/>
    <dbReference type="OrthoDB" id="10262250at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137890" evidence="1">
        <location>
            <begin position="23"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1106432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="115" mass="13027" checksum="CB9B1E85308CF7F1" modified="2019-11-13" version="1">MWNPRVWLVCALSMWFTGTTSTFRSEPQPEICVFCGRDAVIRQCNDITHMETLISVTKTCENLPAGGCNKKIYFHQYICGKCGNYAWINNEPCPNHEHIQVLRSPTDYADPLGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0A5</accession>
    <name>A0A5B0Q0A5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">DEIH-box ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BRR2_6</name>
        <name evidence="14" type="ORF">PGT21_036710</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0A5"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000388">
        <property type="term" value="P:spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18021">
        <property type="entry name" value="DEXHc_Brr2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000133">
        <property type="entry name" value="Pre-mRNA splicing helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000004">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000368">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000254">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000013">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075">
        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075:SF5">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 200 KDA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="261"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1034"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1036"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1106632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1062" mass="120862" checksum="DC28761CF27C5F52" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2P4</accession>
    <name>A0A5B0Q2P4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2P4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="97" mass="10398" checksum="3E71D267015E2FF7" modified="2019-11-13" version="1">MCAPDPNSLPSASGGHHPSSLTRSNNRSDRQVTFAHHEEVCLATADKVVGSYDDRDSGASKVEHVIQSLDILLTGAAVAIRKRILNPAPRAVFHRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2T7</accession>
    <name>A0A5B0Q2T7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2T7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1107214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="420" mass="46280" checksum="1C68F7E9A2E522D7" modified="2019-11-13" version="1">MPPSHWQPPWVVFAPVDSFASAPVSSINNGLASVFPIFPHQFTSPPSFINFHSNLQYFLHIVSQAVLCHLAPAITMSSNNSNVPSSDSSTPVEIPAPQGYQTISNPTPAEEDGYRIYPERGKWSPLFVPKAETPRAADIPVDDTSVVQNGIRYYPERGDWSPLFVPNAETPCAADIPVNPMTDPGVEPAGMAANAPVVSMADHDTDMEATVPENASVDPCPPPVKTFPAPIPLPYGMELIQTGTHFLPQKEKWQSLYDPARNFDLSTIPDKVPVPRGFIDPHPFCDPVFQNPPPGLVLPAFNPLMAANIPSHAPGAHPASASSSTSDASTYPPLSEYLHLGHKDVQDLLSKKNLPPDATCLYTEEDVMRIFEGIAPEFVWLCDDFPPHTLNEFDRRVIFFENMQWHFKCKKSTYLSTRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q315</accession>
    <name>A0A5B0Q315_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_008622</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA. Interacts with transcriptional coregulator SSA1 on chromatin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q315"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="10">
        <location>
            <begin position="23"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="163"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="239"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="403"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="513"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="243"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="259"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="282"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="528"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="579"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="606"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1107274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="719" mass="80706" checksum="D16FAC77A1FE65C0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q677</accession>
    <name>A0A5B0Q677_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q677"/>
    <dbReference type="OrthoDB" id="308690at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990811">
        <property type="term" value="C:MWP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990810">
        <property type="term" value="P:microtubule anchoring at mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052778">
        <property type="entry name" value="Centrosome-WD_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16220">
        <property type="entry name" value="WD REPEAT PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16220:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN WRAP73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="604" mass="66127" checksum="13D601FECF543666" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q681</accession>
    <name>A0A5B0Q681_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_013789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q681"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="340" mass="38186" checksum="4943B12AA8204E9B" modified="2019-11-13" version="1">MDQPSTLKLTGTVVPEFPFSKSAYIVPDAAAIQVAMAMHIDVTRPEDAVQIFNCFASHMKLSHTSTAKDSGQANNLGNLQVLSTYARALDVAGRDSEVYQIWTDFQIDSQVDPDTQIFSTILSSARKITRYSMSPVSNACCAPPNRTRMLYGKWKRTTGTANRQDAWRYGCFAKRPAGYWASLCETVRAPALSGSMWSSAPQRSISAVPQDKRPSKIAATIIPSLRFKTRWTKLIPNRSSFTDMIEMLGLHDRSNLIPLLLSWIRTMGIKPNRDLLIRVYYWLYKSSAITHLLAGLDELDNNWLCEIESYAINNNDEESREGEEGKDLVKHQQGIDVLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6I8</accession>
    <name>A0A5B0Q6I8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025985</name>
        <name evidence="4" type="ORF">PGTUg99_036551</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1122312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6I8"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF434">
        <property type="entry name" value="GB|AAD18119.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="52"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1108801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1122312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="189" mass="21216" checksum="D594A5CC19A46456" modified="2019-11-13" version="1">MLTKNIGLKVVILVAVTRMFNSRSIPSSDPLVSTLKTLHHHIKRTEVVHASCPVCLQDLIADVGEWPGCKHSFHGRCIEEWRNKSISRPTCPVCRGVDGIDQQLPNGSSASIPQRHTNPLLMQDLLEIHPAITPEPRSSRRHISDLIEVPTQCEECGHTLHINRGRNPFMYQHGHTCESCTDFLGFLHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6N5</accession>
    <name>A0A5B0Q6N5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phenol 2-monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_028504</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PheA/TfdB FAD monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6N5"/>
    <dbReference type="OrthoDB" id="1716816at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02979">
        <property type="entry name" value="PHOX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012941">
        <property type="entry name" value="Phe_hydrox_C_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038220">
        <property type="entry name" value="PHOX_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050641">
        <property type="entry name" value="RIFMO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004:SF20">
        <property type="entry name" value="2-MONOOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G13660)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07976">
        <property type="entry name" value="Phe_hydrox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="18"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="232"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Phenol hydroxylase-like C-terminal dimerisation" evidence="6">
        <location>
            <begin position="480"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1108896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="696" mass="77298" checksum="1CCBC2EB7717996E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7F5</accession>
    <name>A0A5B0Q7F5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031973</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q7F5"/>
    <dbReference type="OrthoDB" id="2506783at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="237"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="436" mass="49434" checksum="84039FB7381F76C3" modified="2019-11-13" version="1">MHPPGASSTSITPDSSDVTSSDEQLDPKFVVRNAKSKLKAQKNKDKARKQSSQVVRLSKHSELPHHASALSRSIVEFTKGMLGCYSPSSKLPIPPSTEELEGWENWSSNRQAAVHAAIEAQSEKSHAKNEAERRMMIHNHSSDLKIAIQPVNYNPAPSTKSKIPGIFQTACHDDFKSGGFPRITFDWTEPDLSNSDWNSATADILALNWNKWYIHNPASAKKLKQKVNATGVIQRWLMTMKRKYAKQMAEAEEVDEQHEEHRLENARLRRYRKQRQKIAQMRFNTAIKVFPSKPNICVIFKDVDVVSDYEDSDEINEPPESITPSWRSVSLNRLIPELDQAAILLANKKSRPSVQMLLGRKSSRDANEEDDEWQAVPEKLPFEAYSDKFIADTPILKLRQMGIPAKRSTDHLMEAFHELQQLTMPPSASRSNAMET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA24</accession>
    <name>A0A5B0QA24_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_010076</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QA24"/>
    <dbReference type="OrthoDB" id="2152896at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="492"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="504"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="524"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1110090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="562" mass="61708" checksum="146DCCF35373DC91" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QA75</accession>
    <name>A0A5B0QA75_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001342</name>
        <name evidence="3" type="ORF">PGTUg99_003702</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000226">
        <property type="protein sequence ID" value="KAA1122619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QA75"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1122619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="78" mass="8477" checksum="F643CAA6D4364F1C" modified="2019-11-13" version="1">MTSHPNEANNPSAADAENTTPQTSVYMTLIHLPVDHVKPLVCPAISVPPALTTDWLWIECLSDDCSEHMGNYVESGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBX3</accession>
    <name>A0A5B0QBX3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_030264</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBX3"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001079">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF28">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="371"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="20"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1110718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="542" mass="60063" checksum="53306288CE6C05DA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCN7</accession>
    <name>A0A5B0QCN7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_035277</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. LDAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCN7"/>
    <dbReference type="OrthoDB" id="448051at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019363">
        <property type="entry name" value="LDAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390:SF0">
        <property type="entry name" value="LIPID DROPLET-ASSOCIATED HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10230">
        <property type="entry name" value="LIDHydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="394"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="395"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1110986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="54440" checksum="CC8575AB805730E3" modified="2019-11-13" version="1">MTRSGNDVGSDDSKDIQSTASRDTIRKLLTSYRYPGYPPSDLSIHSSFEQPHLTFDRSTNRFSCNPSDGAHFQPLNSPSDSAAKPPITGPRILVYMIPGNPGLVEFYDRFLSVLCSSFDAIGGCEAVCCGHLGHSLRHNSNLDLRFLDNFKLWNHLFDSSSSSDPTPNHGRQSSREINPPAAGSLADQIEFHCHLVGQILHEGIKDPTHTKLVIMGHSVGGYIATKVLERYPDQVMHIIGLFPTLSHIGRSPNGLRLSPLFSPILLPFLNILQIVFCLTLPKMIICRLITLYYSPISGTRNDHQPANKNQLMKAKDLSEHNLLIILNFILNINSVTAVLKMARSEMKSICELDRQFIHRFNRQMTLFWASHRADEWVGETEITEIIDLLNLYSEPTHDTPSEENHSRLRPTHQPSDLEAPTSIDNQPPPTFEDMVIENQSHVIPTDKVPVWKRMKEGIPHAFCLKDNEVMAKECSALIKQRLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDF3</accession>
    <name>A0A5B0QDF3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Leucine-zipper-like transcriptional regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LZTR1_3</name>
        <name evidence="1" type="ORF">PGT21_000738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000022">
        <property type="protein sequence ID" value="KAA1111247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QDF3"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1111247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="202" mass="23095" checksum="F69FF9A3DA3B42BB" modified="2019-11-13" version="1">MTEHNHIGQYYSNIDWTHHQLMYTFVTRHRIVKAFNKLQPPFPHTGASSAIHRSLINPPTMFFFGGSSIQNQHNDLYRFTFDPNDSKPSLTAGIVNTRGQAPAPRSHAILSATDGHLFLWGGFLYVYAGHNVNRSVQGDMWRIELDSLPSVPFLGVNLVLQRSNLSSCSYKPLCGSFFKLLDHLRWNRRRQRFQRSVGVSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QGF8</accession>
    <name>A0A5B0QGF8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1112053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QGF8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="97" mass="10543" checksum="ACB38669BFBF6346" modified="2019-11-13" version="1">MTPPTVKILKAILLITLRLNNTAHQLELYLLSLHQMSAIDPTCADRFATASVISGYCGGGFPGKSNFSVNHFMKLNMKTSAPASPRKAGHRRPVSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QHZ0</accession>
    <name>A0A5B0QHZ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QHZ0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="338" mass="38714" checksum="A7BC6D8D55C6938D" modified="2019-11-13" version="1">MRFRLSGFFRNIFLFVSNFRPQGIYLLYADHARCISSQFHIFFLTVLISRNLLEAWFKWVHNKSRRASPNPTALHLLLEDFLFDNLSRGGVTTVRESPAHPLDKKKQVVVVSIYRKQISSSITSPPYSINSTQEKEAHHRPHKKGINMMDSDTASMETKRLILKAINDLQRAPHPELTRGGAVREISEKVFSRRKINAGTALLVQVQPDLYPHCGGSSPVLVESLDMNTPENEHKPKLTDALEFANQLGPTLDLLENSIASLAPAIISGTLELSCKIDHEYGLLKRYKCIDLFQKFVHFTFQVSRDLFHTYWVYFQRCVSNPGRLTSPDDDYIKFPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QI61</accession>
    <name>A0A5B0QI61_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QI61"/>
    <dbReference type="OrthoDB" id="2503018at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1112832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="248" mass="26959" checksum="0B62DED27268F3CF" modified="2019-11-13" version="1">MTTRFLKPTSTQLTQRGSKTHPHQRTECTRHINYQIWGPVHSTPGARQQEIFPIATAVSLSGPATQNSRQVTYPQDSQPFPSGDSRALMNGQPNAPLPHPTQLPNSAGGSSKSPSPSTTTTTSLKSQATAVSHHSPSSATEASTTSAASAAPTDEPAPRSALERFAALYKPTNRFEPLAVLLTIVLILAFLVTLMSILKCVAHKKRFANKKDYPTGFRGLTLPEVRQTELVVDKVWLPLRATCRIEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNY3</accession>
    <name>A0A5B0QNY3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_029562</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Regulates mitochondrial small subunit maturation by controlling 15S rRNA 5'-end processing. Localizes to the 5' precursor of the 15S rRNA in a position that is subsequently occupied by mS47 in the mature yeast mtSSU. Uses structure and sequence-specific RNA recognition, binding to a single-stranded region of the precursor and specifically recognizing bases -6 to -1. The exchange of Ccm1 for mS47 is coupled to the irreversible removal of precursor rRNA that is accompanied by conformational changes of the mitoribosomal proteins uS5m and mS26. These conformational changes signal completion of 5'-end rRNA processing through protection of the mature 5'-end of the 15S rRNA and stabilization of mS47. The removal of the 5' precursor together with the dissociation of Ccm1 may be catalyzed by the 5'-3' exoribonuclease Pet127. Involved in the specific removal of group I introns in mitochondrial encoded transcripts.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds to mitochondrial small subunit 15S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNY3"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN GUN1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="692"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="702"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1115016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="792" mass="90829" checksum="D146073C5127DD20" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP38</accession>
    <name>A0A5B0QP38_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_026630</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP38"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45676">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45676:SF41">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022706246" description="RING-type domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="143"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1114889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="257" mass="28744" checksum="AA2E4A134FB8D1B2" modified="2019-11-13" version="1">MNFSTMFSAVLPLMLVIGTLNLAMDLPQSSRPLGFRGSNPSWEMDRSLHWVDEEAGIRSSSRHMMSSEKPTGHELSRADQEQSSQPVVIVLHGQPAEQSTHSPSNLETHSQPLGLDMHCVPQEVSSLEKSNSIPPSSSRIVDCAICLDAMQDDEPLRVLASCQHKYHQLCIEAWLREHATCPMCRVADPVSSATLNQQSRNPRTPLPAPRSRRDIILTRLEITIFVAIIILFMTWFFVAVQQSDAAQLRAHSPPRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QR36</accession>
    <name>A0A5B0QR36_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020028</name>
        <name evidence="3" type="ORF">PGTUg99_025688</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QR36"/>
    <dbReference type="OrthoDB" id="2506007at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="66" mass="7589" checksum="E642F124D8A4F76F" modified="2019-11-13" version="1">MGDIDDALEKLNFEENEIDRSEEDEQQDEINEEEQTEEERQENEANEALDGEETDDGQPAASESQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QUF0</accession>
    <name>A0A5B0QUF0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mitochondrial fission 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">FIS1_2</name>
        <name evidence="12" type="synonym">FIS1_1</name>
        <name evidence="12" type="ORF">PGT21_029142</name>
        <name evidence="11" type="ORF">PGT21_029575</name>
        <name evidence="14" type="ORF">PGTUg99_008218</name>
        <name evidence="13" type="ORF">PGTUg99_028308</name>
    </gene>
    <organism evidence="13 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="13 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has a role in mitochondrial fission.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The C-terminus is required for mitochondrial localization, while the N-terminus is necessary for mitochondrial fission.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the FIS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000035">
        <property type="protein sequence ID" value="KAA1136231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QUF0"/>
    <dbReference type="OMA" id="QFNYAWG"/>
    <dbReference type="OrthoDB" id="421154at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12212">
        <property type="entry name" value="Fis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001249">
        <property type="entry name" value="Mitochondrial fission 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016543">
        <property type="entry name" value="Fis1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033745">
        <property type="entry name" value="Fis1_cytosol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028061">
        <property type="entry name" value="Fis1_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028058">
        <property type="entry name" value="Fis1_TPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247:SF0">
        <property type="entry name" value="MITOCHONDRIAL FISSION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 11 TPR REPEAT PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14853">
        <property type="entry name" value="Fis1_TPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14852">
        <property type="entry name" value="Fis1_TPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008835">
        <property type="entry name" value="TPR_repeat_11_Fis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR008835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1098159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1101749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1116876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1136231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="162" mass="17826" checksum="ED5AE8DEB93C6A42" modified="2019-11-13" version="1">MVTSGTSSTDKLPYAADISLGLSPEELTVLREQYQNEQAKGWISTQTKFNLAWGLVKGNARQGQVSEGIAILMDVYRTDATRRRECLYYLSLGHYKLGNYSEAKRFNDLLLEKEPHNLQAKSLAQLIEKAVTQEGYLGLSLAGGAALVGGVLLASLFSGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVY5</accession>
    <name>A0A5B0QVY5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVY5"/>
    <dbReference type="OMA" id="TIDIHAQ"/>
    <dbReference type="OrthoDB" id="244495at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF27">
        <property type="entry name" value="PHO85 CYCLIN CLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="622" mass="67946" checksum="137B8120DF4C38A0" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QXH7</accession>
    <name>A0A5B0QXH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QXH7"/>
    <dbReference type="OrthoDB" id="2509475at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="219" mass="24061" checksum="56EE27761323BB75" modified="2019-11-13" version="1">MSFNGEWIPGATFSGRAFPDSAVDTSLANASAPRSTDGDSIASEEGSIASDDESKIANDEITDNNTTDNVADLINPNAVAFATGASPPAYRLAALPLGSPENPIEIEDEDAASHPGNNEEMNDYGRLDSPRFDPVEIVPFQTMVVDEEDVLRARERINRWFNNHIRHNSRRVLRGRSRRARTAAGPVEPYWTVNELMDSVLEELDDVLNNASRVELIHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZX9</accession>
    <name>A0A5B0QZX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZX9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="156" mass="17863" checksum="6F80A54594E2DDFA" modified="2019-11-13" version="1">MRLEEWNWMMTKQLGSSKRALLRHEMRRYCAHNQHIHRQCDAASLSASRSRCYSGEIRKGNRRDSSPPATIRLKDNVKEIQPGKTFLKHDNCTALSSLMLKLELLNVHDRETDPNSIKSLVSGGGTPSRRNPSKWDGVPPPKIEAHHQLENDCKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1A8</accession>
    <name>A0A5B0R1A8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED17</name>
        <name evidence="10" type="ORF">PGT21_022976</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1A8"/>
    <dbReference type="OrthoDB" id="10251234at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019313">
        <property type="entry name" value="Mediator_Med17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114:SF7">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10156">
        <property type="entry name" value="Med17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1119347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="661" mass="73313" checksum="8C760B24A6D51372" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3T0</accession>
    <name>A0A5B0R3T0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_037219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3T0"/>
    <dbReference type="OrthoDB" id="2508363at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="333" mass="37549" checksum="C64C2C9D339D1BB3" modified="2019-11-13" version="1">MRRAADYQPSDWELLTPEEQRARALRRVKIREDERIANLLLWNSVKRDHKAKRRQLLAELREKRLAKMSTTSNSSAATTPAAGHTPVARTADNSPITSQTIQTSTAPYPQPLDRGAANQLEERVNRLKLITIQKKMAPVTVERPTEATLAENGSTPAVETPATARATQIVAKTAKNPAEKAADAMDIDPQASSFSADTQLRPKSPDVRVLSKEERIQLLVREHVALWKKFLVDQPLGASEGNRALLTQAQDSQRALQKLIPRAEVEEYVKGWNPWTEKKNLFPVLQQERSGKNRSSSSRSNSYKAPKMNDPRRWKRLMKACHTLESGYMNMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R4U8</accession>
    <name>A0A5B0R4U8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032384</name>
        <name evidence="2" type="ORF">PGTUg99_005534</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1100244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000242">
        <property type="protein sequence ID" value="KAA1120596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R4U8"/>
    <dbReference type="OrthoDB" id="4605274at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1100244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="107" mass="11387" checksum="BC5767479D4E39E6" modified="2019-11-13" version="1">MSVLAASDPQNKMTGFLDSLVESSLDTSSKSIKNHLGQIDNEVTWVQLVGQIFNTNQSLVSNIVLQKICPLSFNVDHLGIPGDVGAYGIALDALLKGRSAQPSTVDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RDY7</accession>
    <name>A0A5B0RDY7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008444</name>
        <name evidence="4" type="ORF">PGTUg99_011769</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000213">
        <property type="protein sequence ID" value="KAA1122974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RDY7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22744">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF11">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN DDB_G0284757"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="217"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1122974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="307" mass="33778" checksum="B675D076848EE0A9" modified="2019-11-13" version="1">MHCCPTSIALRIISPADSSLVTGITHRVIDELHRSGRQDQDRYPKTRHSIFALLHISQCTALSSQSIQAARHIGLLLGLGSFEQTWHDADGLKYKWTYSHKLSDHVKTVVHDSSSRRGQNVWIDQRDGLKHQWTLSPVSNEWIDTALESTITSASTSTGIEGAGGSNCPVSPVRSPEANLSPNETPAQEYATQISSKPPRKADLRDLDSRGLPQYTYTKRLIKGDGNCLYRALSYLANGNDQKTNGEWGGEHALAAAAKVLNKKIILLSLASKDPVFLAYGSAPVDPEAPGLIFLNEHYELLFRKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RMP8</accession>
    <name>A0A5B0RMP8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034646</name>
        <name evidence="4" type="ORF">PGTUg99_030887</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RMP8"/>
    <dbReference type="OMA" id="YCPWNEK"/>
    <dbReference type="OrthoDB" id="407630at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1106415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1126398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="228" mass="25344" checksum="17385EEB77EBF15F" modified="2019-11-13" version="1">MVVFTAQHLFPFSFRDVALGIWHKYPNEHSTHITSVDVLDRSFLPDGTLRTERLISIRQHAPRWIMKLVGGAEEQYVREVIFYKVPSSPDQPPMVLMGSVNLSMSSILICREQIRYQPSHPQASSFFQRADIQAQGTLAIGESWGILGSKLESWARDRFSTNAESGRLGFNNVLTALYRDSQGRGSPGDADSRHQSPSGRSSTNLTADSQKDQQGLPADPTSASIRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RRX7</accession>
    <name>A0A5B0RRX7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002332</name>
        <name evidence="4" type="ORF">PGTUg99_014782</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000145">
        <property type="protein sequence ID" value="KAA1128038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RRX7"/>
    <dbReference type="OrthoDB" id="2509560at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1085297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1128038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="253" mass="27078" checksum="2662CC29B17E963B" modified="2019-11-13" version="1">MASIVPSANHLRRFHICYDISFIPKPVHSLARTSKQITMKSFVFVACLLATLAAATPVELQSRMMPPMGGAGMTTTSSVSDGQQSNTQASNSPFGSSYSHSDSSYHNAQSSTVVQSFGPLMSQIGSVNNMLMNGGMNTMVASQSMSQIANQLQTVFSGLNTCGCFQQGSQVFTMFNDMFGQLSQMLMSMQNTFGASAMGSIVSPFGNVAGSMSQFFSQQSSTNWSSMSSNIMPFINCVSPFVQGFSGLSQMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RVM9</accession>
    <name>A0A5B0RVM9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="3 10">4.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">URA3_1</name>
        <name evidence="12" type="ORF">PGT21_027976</name>
        <name evidence="13" type="ORF">PGTUg99_002406</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>orotidine 5'-phosphate + H(+) = UMP + CO2</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the OMP decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="3 10"/>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1128913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RVM9"/>
    <dbReference type="OrthoDB" id="10263753at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000114">
        <property type="entry name" value="Decarboxylase,orotidine phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 10" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="11">
        <location>
            <begin position="36"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1094673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1128913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="287" mass="31660" checksum="29BC57E8F85C6DBC" modified="2019-11-13" version="1">MSKPDLVTQSYSTRASIHPSPIARRLLKLMDEKQSNLCVSVDVTNKASFLRIVEAVGDHVCMIKTHIDIIDDFDHDLIEKLVELSQRQNFMIFEDRKFADIGNTVKYQYSSGIYKIAEWSDITNAHTVPGEGVITGLAEVGIPRGRGLLLLAEMSSKGTLAKDSYTVETVEMARRHPEFVIGFISQRRLEGLGVEATTNQSDGAQPEDFLVLSPGVGLDVSGDQLGQNYRTPDQVIYQSGADVIIVGRGIYGQKDKSGDSNQAIIAQAIRYKQAGWEAYLKRLASPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RXN6</accession>
    <name>A0A5B0RXN6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035833</name>
        <name evidence="3" type="ORF">PGTUg99_015405</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000112">
        <property type="protein sequence ID" value="KAA1130182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RXN6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033845645" evidence="1">
        <location>
            <begin position="22"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="399" mass="45716" checksum="54CF254C193BD1A9" modified="2019-11-13" version="1">MMCNIFISLTFVICSWKAAQASYTDLVQLANSKESLPVIEENPAQSLAPDWLLFNVVEKVPKKISNQKESLLVGEGDNSSNTLMNSGEDILWKGISSPSLEELTAESDNKKGPRMDRKFKEDVKRIYLINSHNIVGWKTDFDKIHQYFSQSVGFELKPKMATLVRAISNLFEKIVNEQGGHSNDFKAKGENLPTDIPTLIFETWEEIWIGDIHELNLTWKYLNEKDKIKGIFLIRNGINLVLNIFHDLQKHEIITKESLSDFMNTKNGPRLILDYAIMGFHLSVPTAEDAYSNFNLKLSLQHGPATERTAEIFKLLNNETWKWIEFEYLRARFNYSADDLDSTKSEFLVVASPNNKEPSEKGIEEVFIKLATQISQSTFPHGKKCEQNLINLKEKYDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RXV2</accession>
    <name>A0A5B0RXV2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032297</name>
        <name evidence="3" type="ORF">PGTUg99_006446</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000111">
        <property type="protein sequence ID" value="KAA1130208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RXV2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="96" mass="10443" checksum="E1E3CB5D173C59CC" modified="2019-11-13" version="1">MTTAKYLARSIRARNTSDTDYRSFDLTLALQAGLGPDFLTLALPTGLGPSTTTQRQTPPQSSTNQLRIPSSLSLRTASEALNPPLRPHNSTYPIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S054</accession>
    <name>A0A5B0S054_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_028667</name>
        <name evidence="4" type="ORF">PGTUg99_025657</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S054"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033849310" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="43"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="129" mass="14732" checksum="B549918AE5DB1619" modified="2019-11-13" version="1">MNFVFLSLTIITLISQVCGILFEEVFVNSKSYYVFNEKSIISPEMPYISALHNQPFQQGDILKLNCKNNTNSIVQVWTGLQQLIKFKPNEEKTISWRGTNNLMVLTTDNSSDIGVNIYLEAIHSCTYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S8C9</accession>
    <name>A0A5B0S8C9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009774</name>
        <name evidence="3" type="ORF">PGTUg99_027834</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1134117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S8C9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1134117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="120" mass="13265" checksum="D65FC3AFAC3C4552" modified="2019-11-13" version="1">MVPIPGGKTSPSQGKRQLLSGPILFIIMFYVLMLGASRCEGQVLKSPANHPMKREVADDPSHLQSHQSQALKIRNIIRRRSPLKGRVRSPCESSERVINIGNGRTSCRPKHDCQGPNCPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SC79</accession>
    <name>A0A5B0SC79_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PSA2_1</name>
        <name evidence="8" type="synonym">PSA2_2</name>
        <name evidence="9" type="ORF">PGT21_019649</name>
        <name evidence="10" type="ORF">PGT21_021765</name>
        <name evidence="11" type="ORF">PGTUg99_016334</name>
        <name evidence="8" type="ORF">PGTUg99_021177</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000338">
        <property type="protein sequence ID" value="KAA1102288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KAA1135125.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="M1P_guanylylT_A_like_N"/>
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        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
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        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="6">
        <location>
            <begin position="5"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="7">
        <location>
            <begin position="292"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1102288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1105792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1114756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1135125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="414" mass="45830" checksum="182958D6DCC7191F" modified="2019-11-13" version="1">MGSKAVYLLGGPSKGTRMRPLTLDIPKPLFPLAGRAIIWHGIQALSKIPDLKEILLIGFYEDSVLAPFIKQACRDFPSLQIKYMREYEALGTAGGLYHFRDAILKGSPEQIYVLHSDIASSFPFLELKHFHDKHRGVGTLMAVRVSKELSTKFGCIVTNPETSQALHYVEKPESFLSNIINTGVYLFDKSIFDEIKAAMDLKVKQTADDPLSRQDDQLRLEQDVISPLADRGKLYVYETKSLWKQIKTAGSALPANALVLESYKSNNPVLLRRRSPTIIAKTPPPNLLGPEIVEPCYIDETAVIDPSAKIGPNVSIGANVRIGFGVRVKDSIVLDNSLLEQNSCVMHSILSEDTKIGPWARVEGCPNTSDANPLKFTISVLAKDVEVKSEVHVRSCIVLPHKTLTRSFANEVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SH55</accession>
    <name>A0A5B0SH55_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_002910</name>
        <name evidence="5" type="ORF">PGTUg99_008942</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000011">
        <property type="protein sequence ID" value="KAA1137172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10001928at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF2">
        <property type="entry name" value="NEGATIVE REGULATOR OF SPORULATION MDS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="97"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="674"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="879"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="675"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="694"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="730"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="879"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="905"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="950"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1096028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1137172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="984" mass="107255" checksum="F3AAEA7B2195E5C7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SKS1</accession>
    <name>A0A5B0SKS1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_001914</name>
        <name evidence="6" type="ORF">PGTUg99_014705</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1095994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000011">
        <property type="protein sequence ID" value="KAA1137204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SKS1"/>
    <dbReference type="OrthoDB" id="5983572at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050670">
        <property type="entry name" value="STAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929:SF7">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="108"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="45"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1095994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1137204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="277" mass="29731" checksum="B39480C9503C3E1A" modified="2019-11-13" version="1">MAQHHPENSDSIYTAHLINQIHANLDLLLQQAKISRNKVDLIKEALPQSSSRADSGLPQPSFPNNRTASPPQPTPSTQADHPKSQPPPQHLPTSFPTPPPPPAHQPPPQCKQARALWAYHGSAADDLSFEKGDIIVILAEENPDWWRGQVINSQQPGGLFPSNHVEVISVPSQSDHSPIPQYRRNQSIAPYPPGPPQGNPGYQSPAYSQAPQYGDYKAPQAAPPPPLQHTQSAPPVNITVEKPKKNILQGRFGQALAGGAGFGAGSAVATHVVNAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJ89</accession>
    <name>A0A5B0LJ89_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJ89"/>
    <dbReference type="OrthoDB" id="2513151at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="437" mass="50379" checksum="719BCFCD0851B729" modified="2019-11-13" version="1">MSNPTNPSEEIDKMPNTADQSEEMSDESDLSEETDDISDTTDRSVSGQSDLSEGTRQTWQQGGVVYRQFSGLMDKCRAEPMSIQESASIDRVESKKDFLNQLHSTLLPLLQQHIVKLASEFKLDMLWGDRDAKFKTLLESQSELNHILDQINYAKYSICPAKSEPDASTCDQNLKEFKTYRISILREKLNSLLSQLELTFQFCRYLLEELPTSEYSMYCSDVFFLKRCFASGTSECCNTIDSTIRWMNRSDFYLIRDQWPDWIYTINEKFDEFMKLSGSDLISPNQKLRHKLLKLAKLVIPVIKLCRIFFSKLSKQVLSMTWLPLFTELSSQQIKTLRILPGYINSHLKNFLLGLKQTTNNNRATSASSLLMSAQEHSKDFQISVDLITSHIVPILPRTDDFPSQDYFRNWLGTWNTEHLVATENLVQAVKLIGRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLD8</accession>
    <name>A0A5B0LLD8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLD8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023000174" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15838" checksum="E7E534EB91665981" modified="2019-11-13" version="1">MIPKIGTLCVLMAFITTATASSIGCIIGRCTGTATEVPLSEFPAYPAKDTCGEKIGSNPPCRVQRAKEYFKCGGCKSVIVKNGPLQGGEQERCDHLNLHVYIQWKPIPEESSVEDEAPARSSLNRMTYYPKPPFDPQTRHFMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LMH1</accession>
    <name>A0A5B0LMH1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002745</name>
        <name evidence="3" type="ORF">PGTUg99_014714</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000443">
        <property type="protein sequence ID" value="KAA1079966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LMH1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1065545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="82" mass="8950" checksum="FCCB40D92793FBFF" modified="2019-11-13" version="1">MPALTLAHWPQVSKTQPQQQQPHILPSEGEQNLEASVPTTLPRETNRERPSARSSESPNKLNPPPSPLTLVVPLKPQSGVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LQ61</accession>
    <name>A0A5B0LQ61_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_033872</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LQ61"/>
    <dbReference type="OrthoDB" id="1898221at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF149">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="45"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1066571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="218" mass="23586" checksum="CFDC4664EB54F4CC" modified="2019-11-13" version="1">MANQTIYRSPWGNTLPQLEKRLNIWDFMRSTMSAEALSSPEPRCIDVGSGESWSLSQILDHAETLAKFLVSSFGGRRSTWNTGGANVDYEVITVGVLAPNIPAIPIVAYSCMAAGLPVTPLPVGSSSSEIAYLVCLAQCEIVFVHPSQLETIKTSGYPTERIILTEPFEGWDGQILPDLLVIARSLPEFTIDGKHPMPKHQVALVVFSSGSTGNPKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRP6</accession>
    <name>A0A5B0LRP6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014963</name>
        <name evidence="1" type="ORF">PGTUg99_012379</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000507">
        <property type="protein sequence ID" value="KAA1067101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRP6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="60" mass="6831" checksum="3B816CA1305C1F5B" modified="2019-11-13" version="1">MVVQSRINSQRCFDSNSVLEESSRRLIPYFSYLTDSVSDPSAQFLVLANSHRSHLELSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVH3</accession>
    <name>A0A5B0LVH3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVH3"/>
    <dbReference type="OrthoDB" id="10282494at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022936738" evidence="1">
        <location>
            <begin position="20"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="122" mass="13442" checksum="48E87C79A43CA644" modified="2019-11-13" version="1">MRTVVAVILFLALLQSASALPTLAKRGEAKPKGELQAKYAVPNDGRLCVLFGMNCDTVPPQPSTNYWPWPQSPDDYNPSTWPFWTSPSQTIPYTPNDSPYTDAMIGQSLKAVKDNNKKDAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LVP1</accession>
    <name>A0A5B0LVP1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stretch-activated cation channel mid1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MID1_1</name>
        <name evidence="3" type="ORF">PGT21_022423</name>
        <name evidence="2" type="ORF">PGTUg99_029176</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LVP1"/>
    <dbReference type="OrthoDB" id="5405745at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024338">
        <property type="entry name" value="MID1/Yam8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142:SF1">
        <property type="entry name" value="AEL197CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142">
        <property type="entry name" value="MID1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12929">
        <property type="entry name" value="Mid1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366058" evidence="1">
        <location>
            <begin position="19"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="570" mass="61630" checksum="339D96812A5C701E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M0A5</accession>
    <name>A0A5B0M0A5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M0A5"/>
    <dbReference type="OrthoDB" id="2527403at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018803">
        <property type="entry name" value="Ish1/Msc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33939:SF1">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33939">
        <property type="entry name" value="PROTEIN CBG22215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10281">
        <property type="entry name" value="Ish1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023121317" evidence="1">
        <location>
            <begin position="22"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="721" mass="79703" checksum="CEDB4EDF677A7302" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M165</accession>
    <name>A0A5B0M165_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M165"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="219" mass="23557" checksum="D2AFB722009E579A" modified="2019-11-13" version="1">MALSRPLAGLTGREVESGYVANLAARSRVLMNKISTRLPAPSPSQEPIAIRLPPLQFTFCSSIILSARMSSNPNNNLLSTAAQMKRNNANLAKENAEDPTEEGPDRINPHLNPNLPTHTQSNPVPTNTNPIPIPPKPKNPATAKAPIGSNSHQTTTKSTAPPNTSQKHPMSLEEVASSAERHRQPGKQAKDLTAILGAKTIDVEKEHMERTIATNAALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1C6</accession>
    <name>A0A5B0M1C6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1C6"/>
    <dbReference type="OrthoDB" id="2501672at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="137" mass="14618" checksum="BEBCEF265D565415" modified="2019-11-13" version="1">MNTNTNQRSGGRVSDDMRDSSMNPTKDMMSGMDSGMDDNMMNRDRQGEDQGLMGKMSSMVGGNKNDNNQSHKMMGGVAGGRDSNRGQQEASMGDKVKGTFEQAKGKVMNDPHTARKGDMRKDPAGAGSNDFMGGAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3A5</accession>
    <name>A0A5B0M3A5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3A5"/>
    <dbReference type="OrthoDB" id="2499564at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="347" mass="38623" checksum="5C9DC568D64B1F37" modified="2019-11-13" version="1">MFKGEELDENFVYTDHDMVSIISRIVNENIDCFEIEEVQTGMCNFHIPFSLELDDGRIMTRTCAMFIATYERLKFFNEDSKAEAKSPGWEEIVHKTPIQELFSMIEFLREQARDTSELEDLPKESPSLDKNLLPEEPSQLTKTEQADSSFLDKEKSSGSLEATADLPSDNLAQKSGDPQLIEYSIEIKDEGVASYAGKRPPGTIAPMEGGGLNGLIEVLQRFSDGPDSTEPKKLPSSRPKVAPLDLSAEVKKKPKTVETPTSALPLPLSSRLNAALLSPQSYAKFRDAALPLEELYRRNGKDREKLERFLAAHAKDVSSKCGFIRSCDSSLRRKSGSFNDGSATRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3W6</accession>
    <name>A0A5B0M3W6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5593376at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="888"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1301"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="617"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="905"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="953"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="971"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1302"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1341"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1408"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1434"/>
            <end position="1451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1460" mass="157267" checksum="E3D9F83886C59F37" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M4B5</accession>
    <name>A0A5B0M4B5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FET5_2</name>
        <name evidence="10" type="ORF">PGT21_005081</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimers with GPN1 or GPN2. Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M4B5"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000552">
        <property type="entry name" value="GPN-loop GTPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1071361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="306" mass="34511" checksum="812071C25CA4EBF5" modified="2019-11-13" version="1">MRYAVLVCGPAGSGKSTFSSTLITHAQTIGRPIHLFNLDPAAEEFEYEPSIDIRDLISLEDVMEELELGPNGGLVYCFELSQTLDRSESKNQVNGDFLLIFRYLLKNLDWLQENLNSYDDDFLIIDCPGQIELYTHFNIMQKIVQVLTMEFDFRLCATYLLESNFIADRPKFFSGVLSATSAMINLEIPHINVLSKMDLFKTGRTAAGTIAQIGPKELERYLDPDPDLLLGEVNEKTNPKFHSLNQAISHLIQDFNMVSFLPLDITDDESIGSILSHIDHAIQYGEHEEPKEPHDMDGGDFNAADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5H4</accession>
    <name>A0A5B0M5H4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_027494</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5H4"/>
    <dbReference type="OrthoDB" id="444135at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001209">
        <property type="entry name" value="PRK00175.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF16">
        <property type="entry name" value="SERINE O-SUCCINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="123"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1072085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="505" mass="56018" checksum="11F1E96B3A862A64" modified="2019-11-13" version="1">MCARPALSLPVSRPSTPSLFYSNNSRPYVPRRSPIRRAIHVQSTAKRFKLDAKDSPVTVYRSYSKHPSPRAPDGPEPTYSKLVSGYQIYHHSAPFKLDYGHSLPSFDLAYEVWGKLSDDKDNVILLHTGLSASSHAKSHQENRNPGWWEKFIGHGPSYPIDLDRYFVICTNVLGSCYGSTGPHSTNPLTGRRYATDFPIVSIFDMVRAQFKLLDHLGIDRLYASLGSSMGGMQSIAAAWLEPSRVGKVVSISGCGRSGPSSIAIRYAQRSVLMADPNWNGGFYYDSAPPHVGMKLARQIATITYRSGPEWEQRFGRSRRSVLNQHGQTSLSPPTLSPDFLIETYLDHQGEQFCLKYDANSLLYVSKAMDLFDMTVEGLDELERYQTGAVNSPEESSFPNQPGRDQAGRVTSLSSAGDHKIVASLARTFQKFRSTQEFLILGVQTDVLFPITLQREMAEAIRSSSSNIPPHQAPAVTYFELHSPFGHDTFLIDLNGVGGAIRGFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6P6</accession>
    <name>A0A5B0M6P6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Triose-phosphate Transporter, variant 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CAS42_1</name>
        <name evidence="8" type="ORF">PGT21_034418</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6P6"/>
    <dbReference type="OrthoDB" id="18894at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="74"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="369"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="392"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1072442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="437" mass="49421" checksum="42FF12F99CAB1D99" modified="2019-11-13" version="1">MNSTENQQDYNYSTATPTNYYEQQQPQAELQTEEHDLERSTTSKPRNTITPSTKATFWKNALINASFIASWYLFATLISLYNKWMFSPDHYNFQSRTRPTAHDYLRKALPCGMASGLDIGLSNTSLKTVTLSFYTMCKSSSLAFVLLFAFLFKLEKPTYKLTGIILLITAGVVLMVSSETQFDFWGMVEVLSASCLGGLRWSLTQILLDKQSMGMNTPIATIFWLAPTMGLSLSFCSLIFEGWSNLLSEQAFFGDLGKSFMTMTYIATAGVLAYLMTVSEYFLIQRTSVVTLSIAGVFKEVGTIFLSTVIFHDIMTPLNISGLGITLFGIGLYNVLKYKQSLKKVNLSDGRDRQGSIVLTNRTKEDSKIYSKLEDDEEEDVDLDSGSEEREEDHQHHDVHPNNDYEDLDRDRDDSIDSNIDPRRRLMLMTEKAPSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7H9</accession>
    <name>A0A5B0M7H9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_025066</name>
        <name evidence="4" type="ORF">PGTUg99_017351</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1066212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000477">
        <property type="protein sequence ID" value="KAA1072685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7H9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137183" evidence="2">
        <location>
            <begin position="20"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1066212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1072685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="162" mass="16540" checksum="6239DF6F42A73B61" modified="2019-11-13" version="1">MRSFLVIAIFLTLLQSSFARPLLDGQTESLKATTKRAEEGPVPSNSLGDLTNDQATAFNASARPVGAGKRASIEIPTASNQLANANSESEASPQVEGSGTSATPAEFGDSEDAGGEQDGDFALPDGYSGQDEMPGILGVDGKPIPPAGYQAIKGPGASDLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8V3</accession>
    <name>A0A5B0M8V3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_018962</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8V3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023082052" evidence="2">
        <location>
            <begin position="20"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="633" mass="72192" checksum="4D7AD60F60984226" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MB56</accession>
    <name>A0A5B0MB56_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MB56"/>
    <dbReference type="OrthoDB" id="10355659at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="285" mass="30775" checksum="9CFCC685B152EFC8" modified="2019-11-13" version="1">MTEDYSSDFPVPNASPAAPSSPEAVSMDLDVVVEAPPSQSIPAPAPISSPRVVPSFRIRGAANAAPHSSPTPLVPANQNGVGARARGRARNRGQARDRGLDPVRVRGRGCGFVPAPFRGRGHPSGSNNLPTGPVISNRAVVGRGATPPPAPRGSRLPTPQLSPGGPFDRHGSLTSADNSWVEPHVRTRTRVPSPAPFGFYDVDDYHRLAPHQVAALLDRRPDFGRYDQHQFAPQLYTAFYQDVASALVGHFTRHPATNTFEREGRVALFRFVGRRYRSALRRVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MCC3</accession>
    <name>A0A5B0MCC3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MCC3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="78" mass="8467" checksum="BE48E19BA9028E04" modified="2019-11-13" version="1">MSFTPLLTDVHIGPLALCPKAMTAQAAKSKAIDDSDSDEEEETEDDSEDSVPDALTPQVPKLNVGNLARLLFKKLLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MD76</accession>
    <name>A0A5B0MD76_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_010884</name>
        <name evidence="3" type="ORF">PGTUg99_023055</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000474">
        <property type="protein sequence ID" value="KAA1073920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MD76"/>
    <dbReference type="OrthoDB" id="2501604at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1116367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="281" mass="30676" checksum="25088114D2D68940" modified="2019-11-13" version="1">MAEKSTSTPPSKLQATSARLQTSIRREPLAAFRVFFFVLIWTLALANVVIVVQEFQDSLPFIIGVGGRRRIGIEDSRRQALPVIIAPCVTLIILIPIAVGLPLFGIAGFLSRYIIEALWTGLIVVLTLAGAASLSPQPTSPLLPRTLQYNLSLVLALSFFIVIFLSFYNIILLCRISFLLGKGIPNPLHAPVPSLLFRMTTQDSQSGDEQSEKVEDQTQQATPAEIARVDERLENGSIKTLSSGWDDEDDGKPNKAPKNESGFAKARAMFSSPLKNSPSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MG92</accession>
    <name>A0A5B0MG92_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acid phosphatase pho5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MG92"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF42">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="44754" checksum="D95BDBD43BC38E2B" modified="2019-11-13" version="1">MDLEKLPVYQVIPPSELQEQDYLHAAHPSPPFESSTDRQARGTSQFRKTIRDVALGFLIATAFWTIPKATRVSWPSARRCMGRFFGHGHHHHHHHNHHHHHNHGHHHNQNLFPSGKEIGYQGPTETGVEPFVAVTADVPPEQKSYFPLVSPAQSSHDAFDPVRSWGYLSPFHSLPSNAFGLEGSSGKVPNQCELTQVHLLHRHGARYPTSSKEPAGFATRLKAAGNYKASGNLEFLNSWSYGLGKEVLTPFGRSQLFNLGVGFRQKYGHLLNKIAHPKAKLVFRTTSQHRMLHSALNFAAGFFGLPFKSQYHQSIVIEAPGFNNTLAPYFTCNNSEHASKTFVKTIMSNWSEIYLAEALPRLQANLTGYQLTFEDVVSMQKVNIVIFYFERFRLGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGK1</accession>
    <name>A0A5B0MGK1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_003635</name>
        <name evidence="2" type="ORF">PGTUg99_025193</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGK1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1135602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="92" mass="10165" checksum="3E6DDE729ECD3305" modified="2019-11-13" version="1">MSEVWTQGAKLEMTALKPSRELPKTSQHIPLEGSWRAEVSVAEGQDGCYEPIPPDGSRRHGFSPESLVRAVDLNQLPCIQATQLYQASSKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MHL5</accession>
    <name>A0A5B0MHL5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007445</name>
        <name evidence="2" type="ORF">PGTUg99_011063</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000038">
        <property type="protein sequence ID" value="KAA1135518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MHL5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1135518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="143" mass="15945" checksum="F17D7271C6F17BCD" modified="2019-11-13" version="1">MHFVVSDGRRKREGVILGRIAIIRKKAVKLTSIVVGMDRTLQVSLRVQQSSNLIRGSTKPPSRPSSIQWLETSTAARAVFILCLSFRTSFQSAGANLRAENLDQPTSKEKSGLKFNSNQELRIGQVLFEPLVCLLDAWGRLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MI92</accession>
    <name>A0A5B0MI92_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030402</name>
        <name evidence="2" type="ORF">PGTUg99_029705</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MI92"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="101" mass="10926" checksum="12FCC4853AB141E1" modified="2019-11-13" version="1">MRTTHETDRPIGPIGAPARDFSHHRRPPPKPVAKPRPALPSSGSARPQGMVFSPDELPQDLKKMGRNGLPPASGRLPAPLEIFQATRICIIVRKSSSTSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MJ95</accession>
    <name>A0A5B0MJ95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016380</name>
        <name evidence="3" type="ORF">PGTUg99_029080</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MJ95"/>
    <dbReference type="OrthoDB" id="2495482at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036366144" evidence="1">
        <location>
            <begin position="23"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1076702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="213" mass="24133" checksum="D402C6E60A7148FB" modified="2019-11-13" version="1">MQFLSALASITLLVSFPLTLHAQNVPNDYIIKEYTDPSKPYLQVLDAKAKVAYTFEKAYNFPSQFKNTVTIADPSLKPIFSMVSSDDQCRLKTHYAQLGGPAAKDNLPKREYKYDNSRAFGKILWRFNFYSDGAGAREYYKFERNRTNKGGRIYKVAAGQPSQLVGLLRFQVRRDNWVGPDTVNGIKTFTLSCIDGAPLPELVTLLGMVFYRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MM85</accession>
    <name>A0A5B0MM85_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MM85"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="88" mass="9864" checksum="3EFA5CCDD067BD28" modified="2019-11-13" version="1">MWGREQSIEVVDIRLLNNLHQQLSLRREAIGAFAFRKPQLDLASNLGLDLGFLVRQWQAIDTHLLSRAKATLKQHVVTASASIRASSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMS9</accession>
    <name>A0A5B0MMS9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMS9"/>
    <dbReference type="OrthoDB" id="2504485at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="147" mass="15778" checksum="312633B05D2D7AB7" modified="2019-11-13" version="1">MYASICPIGAPSRDPAVKRNPSKKAVPKCPTPPPSSRSVRRNGMFLTGPPAPPPPSSRSERRKGMIFTNPPPPPPASSRSERRNGMILTTNELPPQLKSLLAADCPPAPPSPPPRPSRRQGMILDPNDLPRDLKELKFVGGNATKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MN02</accession>
    <name>A0A5B0MN02_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Triose-phosphate Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CAS42_4</name>
        <name evidence="8" type="ORF">PGT21_035300</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MN02"/>
    <dbReference type="OMA" id="WLMKSFP"/>
    <dbReference type="OrthoDB" id="18894at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="66"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="397"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="437"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1078457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="465" mass="52466" checksum="6A675C49F619A798" modified="2019-11-13" version="1">MNSTENQQDYNYSTATPTNYYEQQQQQAEQQTEEHDLERSTTSKPRNTITPSTKATFWKNALINASFIASWYLFATLISLYNKWMFSPDHYNFQYPLFVSACHMYIQFGLAALTLALFPSIRSRTRPTSHDYLRKALPCGMASGLDIGLSNTSLKTVTLSFYTMCKSSSLAFVLLFAFLFKLEKPTYKLTGIILLITAGVVLMVSSETQFDFWGMVEVLSASCLGGLRWSLTQILLDKQSMGMNTPIATIFWLAPTMGLSLSFCSLIFEGWSNLLSEQAFFGDLGKSFMTMTYIATAGVLAYLMTVSEYFLIQRTSVVTLSIAGVFKEVGTIFLSTVIFHDIMTPLNISGLGITLFGIGLYNVLKYKQSLKNVNLSDGRDRQGSIVLTNRTKEDSKIYSKLEDDEEEDVDLDSGSEEGEEEHQHHVAHPNNDYEDLDRDRDDSIDSNIDPRRRLMLMTEKAPSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNS4</accession>
    <name>A0A5B0MNS4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_021164</name>
        <name evidence="8" type="ORF">PGT21_030484</name>
        <name evidence="6" type="ORF">PGTUg99_012831</name>
    </gene>
    <organism evidence="6 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000449">
        <property type="protein sequence ID" value="KAA1078605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNS4"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032156">
        <property type="term" value="C:septin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000196">
        <property type="entry name" value="Cell division control 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="5">
        <location>
            <begin position="56"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="385"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="385"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1078605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1084211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1119630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="433" mass="49978" checksum="E5D3C4E0E551F822" modified="2019-11-13" version="1">MSDTDHYSQTDQQGNQSNISQEHSEQPLANVVRKKLMGYVGFANLPNQVHRKSVRKGFHFTIMVVGESGLGKSTLINTLFNTTLYPPKEYPGPHAERPRTIAIQSISADIEEAGVRLRLKVVDTPGYGDFVNNAESWRPILENIEARFDGYLEQENRVNRAKIVDNRIHACIYFIEPTGHSLKLVDVEFMKCLHTKVNLIPVIAKADTMTDDEIAAFKQRILADIAFHNIRIYEARRYEKEDEETIAENEEIMRKIPFAIVGSDSEVQTADGRQCRGRQYPWGIIEVDNEEHCDFVKLRQMLIRTHMEELKDHTANVLYENYRTEKLLSIGVTQDHSVFQEVNPAAKMAEERALHEAKLAKMEAEMKMVFQQKVAEKEAKLKQSEEELYHRHRDMKEALEKQKADLEDKKRRLESGRPLTPEKTKKKGFLSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNX1</accession>
    <name>A0A5B0MNX1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Translation factor guf1 mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">GUF1_1</name>
        <name evidence="1" type="ORF">PGT21_016884</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNX1"/>
    <dbReference type="OrthoDB" id="1074at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1077706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="148" mass="16435" checksum="F5BEB1D925D8E7A8" modified="2019-11-13" version="1">MENPRCLIDCSNSPTLAIPSELLSSIHGSIIFRALSLSSRSPMIRSKRPTILGDTLYAAKTPFAQIHPVNRSIETRQLMVFAGLYPIDSNASFYGYDRMINMPSRSGLDRRSICGGRIEISKGKTWMVKKPVDFPSVCNRADGVEVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPE7</accession>
    <name>A0A5B0MPE7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPE7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="129" mass="14205" checksum="576C5956945C1440" modified="2019-11-13" version="1">MWLTAGLGCALEERHLFRSANPTNRWLRRVKPSIPDQYIDPVGQPGRATPRSTFSTSLPPSFVPSLPSLTQAGTVASSAELSTEMKPEAYTCPTRLLRGFSFRLADCRSAHKVDKISTSEPQSAHRHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPH5</accession>
    <name>A0A5B0MPH5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_033642</name>
        <name evidence="2" type="ORF">PGT21_034664</name>
        <name evidence="4" type="ORF">PGTUg99_018770</name>
        <name evidence="3" type="ORF">PGTUg99_021523</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000308">
        <property type="protein sequence ID" value="KAA1107000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000245">
        <property type="protein sequence ID" value="KAA1120360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPH5"/>
    <dbReference type="OrthoDB" id="2512769at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1120360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="58" mass="6560" checksum="FAA19E5E7700C6C6" modified="2019-11-13" version="1">MFCNLTPRAVNLMIGQRDPHQPHTRVISEIFSAIEKLERWVGQRSGDKLIGSNVQGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MR35</accession>
    <name>A0A5B0MR35_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_034171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MR35"/>
    <dbReference type="OrthoDB" id="10278644at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022822792" evidence="2">
        <location>
            <begin position="20"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1078370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="225" mass="23560" checksum="433E4A303020E693" modified="2019-11-13" version="1">MNSFTIFAALLVLSLEINAHSPDSYMAQPHSHLPASEAYNSPSLPSHAVVPTHNTTPLKGSNSQISNGSNGYLEPVFSGHATPKPDSALHEGAHSGAYTNPGATARPVQPTPSLQGMIPGAFDTKHAPVLHPAGGPKEAGSSSGLPDYTPSSEPFSGHQAGPGSLGYGAPKRDVIAARAADLQPRTSRCIIPRRCRAPVHCVSPTSCRALAKYCWKKASRCGYTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVI8</accession>
    <name>A0A5B0MVI8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVI8"/>
    <dbReference type="OrthoDB" id="2509522at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="440" mass="51089" checksum="6327061DD141748A" modified="2019-11-13" version="1">MEFRSQFDLTKKSLSQLPAEIKRLIVEQTALVTENREWEGHKFFKLALVDRSFYELCSPFNWKELNLRFCGRPVLEDLIHDILPRQAKHVQSILMGLHPDDVPDQKNAACNSETIDDSSDSKAIDSDDEFPEEELVYILETCTNLTKLEIGFEPTSLDEFGNFIIDHLRPITMITPLISQLSSLTHIHLDNYSYQPRILFSEEFLVKLLEKMVHLVYIRVNYIEASVPICDFCECSQSIQPSVSPLGVHLASLPSLKAIVFAYADCFTSDWSKLEWKGALEELTLHYCFKVSLRALHAFCSLLKNSLVKLSLYYAPLQRLYVFNDYSVEFLNLFRESSNIKHISLKNNVEIRLEDLKNLMNHRDPVWRQLKSFVLCDDRGYDEHEWPTPEEVSDLIAHGAKASVKVEHGFVLDDGKRWWLNDGEDQWRFEEEIEESENST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWF6</accession>
    <name>A0A5B0MWF6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWF6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="11788" checksum="7BD485DFDD62C52D" modified="2019-11-13" version="1">MRLSHRIGVSDAGPRIASPALAKPMRSIGIASISDAIADTLLAMPILSAGIASVPLGTTPLRDSSPSYIDEHLIPNDRHHSSSYLSVHSLPHISPIKFRSNGRSADLGRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWH7</accession>
    <name>A0A5B0MWH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033574</name>
        <name evidence="3" type="ORF">PGTUg99_018731</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000111">
        <property type="protein sequence ID" value="KAA1130258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWH7"/>
    <dbReference type="OrthoDB" id="2506246at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="903"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="928"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="384"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="496"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="534"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="616"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="699"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="758"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="809"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="827"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="874"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="904"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="928"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="946"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1130258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1154" mass="128429" checksum="D73CD5356814F32D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2Q7</accession>
    <name>A0A5B0N2Q7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_005510</name>
        <name evidence="2" type="ORF">PGTUg99_035630</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2Q7"/>
    <dbReference type="OrthoDB" id="10385125at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="199" mass="21414" checksum="E318B593248DEF12" modified="2019-11-13" version="1">MTPPGKCSDEPVQRLAGRPCPRAARTNPCEDSSDRPVQPALALPHRSDNDNLRSGRLLSDLLPSSSSSSLADRSGLEDNRLKHPFPSELADELDPTLLSQLSLSPAIGPAEQLDPPAIFVAVECPERAASWQILPDLFGLTSHSKDGLSESTSLSSLQEPPTTPVISHTAPLIRWSCKLRKSMGAEMNQCAHAECGYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2S4</accession>
    <name>A0A5B0N2S4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_001542</name>
        <name evidence="3" type="ORF">PGTUg99_003229</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000285">
        <property type="protein sequence ID" value="KAA1111370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2S4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1111370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="194" mass="21448" checksum="6CB405EDAECCA761" modified="2019-11-13" version="1">MDPNAFGGPQGGGQQPAVEGDIRQQHQPQLPIPPQLPIPPGHQHEHDDRVNILSQEVLGLKNNINTMMAMMQSMVARDSDVNANRGRQTPPHQHPPQTQSSLGHNQHTADLTQPFEAQPRMEPMKVPDLWFSGDSGQLLAGETWARGRQTQVLSLSCFGGSVTRFEILTLPQNFLDVWSGIFKDLPDLVDQAKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2Y3</accession>
    <name>A0A5B0N2Y3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_020574</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2Y3"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
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            <end position="217"/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1082926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="974" mass="109205" checksum="B1AFFCC42B93B37B" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N593</accession>
    <name>A0A5B0N593_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGT21_003916</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <end position="578"/>
        </location>
    </feature>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAA1083683.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="584" mass="62708" checksum="FFD8CAE5F327614F" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <location>
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            <end position="172"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1084635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="172" mass="18789" checksum="D4CDFBEF87B6CCF3" modified="2019-11-13" version="1">MEALRKLNFLYGNGGGWFSSSSRSSSSSSHRRVKSSSSSSCRRVKSSSPSSHRRVKSSSPSSHRRVKSSSSSSRRRVKSSSPSSHRRVKSSSSSSRRRVKSSSSSSRRRVKSSSSSSRRRVKSSSRSRVKSSSSSSHSRFKYSLSSSHSMVKSSSSSSLSRLKSSSSISSEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N9L5</accession>
    <name>A0A5B0N9L5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005927</name>
        <name evidence="3" type="ORF">PGTUg99_009989</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <location>
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            <end position="69"/>
        </location>
    </feature>
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        <location>
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            <end position="107"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="107" mass="11863" checksum="77BF2F3F80DB53CE" modified="2019-11-13" version="1">MGNTRKRHKDRASSTTSISSSPAQSKGPSHKKRNNAVVDTDGDYEVEREDTDVDHTTSASAQNLEKDQDQLDAEELKKAMKVHKNQQSACYASYNPPQLSDQLDKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NAV8</accession>
    <name>A0A5B0NAV8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001609</name>
        <name evidence="4" type="ORF">PGTUg99_010631</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        </citation>
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            <strain evidence="3">21-0</strain>
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        </source>
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    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="Chitin_Synth_Reg"/>
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        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF3">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="216"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="700"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="866"/>
            <end position="893"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="14"/>
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            <begin position="15"/>
            <end position="24"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="97"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="137"/>
            <end position="150"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
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        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
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            <begin position="267"/>
            <end position="286"/>
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            <begin position="312"/>
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            <begin position="711"/>
            <end position="720"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1085280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1128020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="893" mass="98833" checksum="FE094BB2726D8E00" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NBZ8</accession>
    <name>A0A5B0NBZ8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">pH-response regulator protein palA/rim20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RIM20_4</name>
        <name evidence="2" type="synonym">RIM20_1</name>
        <name evidence="2" type="ORF">PGT21_025455</name>
        <name evidence="3" type="ORF">PGTUg99_008806</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
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    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="83"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="213"/>
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            <begin position="528"/>
            <end position="575"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="98"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="575" mass="64485" checksum="73A847B84A1BF715" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NCU6</accession>
    <name>A0A5B0NCU6_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1085559.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1085559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="63" mass="7343" checksum="2640721FA06DB1CE" modified="2019-11-13" version="1">MGNFKYNNDGICSYAPIYLFQHPDYCNFNLFLLAFIMVSGYGLLLLGNRLDAEKEKEKESKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NET2</accession>
    <name>A0A5B0NET2_PUCGR</name>
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        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ORF">PGT21_026403</name>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087236.1"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF6">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1087236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20749" checksum="50B8C2EF71BAAC9E" modified="2019-11-13" version="1">MHALREIAPGEELSISYITLVQSREKRRKSLHGTYGFHCGCSQCSLSDAESEASDQRVEKIRELWDVISDWDSSPPSTPAMADEILELFKAERMDVVMEEPYTMASLVYNSWGLTHQARQFSALAISYGVYTHEKTWLETSSHLPLIYDPESHWSYNIGKKMDAEVQTTQTDIAHYFHVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NG92</accession>
    <name>A0A5B0NG92_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_003444</name>
        <name evidence="4" type="ORF">PGTUg99_018033</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061499">
        <property type="term" value="C:outer plaque of mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902412">
        <property type="term" value="P:regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031028">
        <property type="term" value="P:septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052574">
        <property type="entry name" value="CDIRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47566">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47566:SF1">
        <property type="entry name" value="PROTEIN NUD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="19"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="137"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="286"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="339"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="623"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="828"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="878"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="972"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1010"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1099"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1792"/>
            <end position="1872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="53"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="304"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="650"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="681"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="747"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="771"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="832"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="880"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="993"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1010"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1099"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1153"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1227"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1255"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1792"/>
            <end position="1815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1841"/>
            <end position="1852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1088351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1105340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1872" mass="210030" checksum="0257B5F9B586F2BF" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGA5</accession>
    <name>A0A5B0NGA5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="11">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MNS1B_7</name>
        <name evidence="12" type="ORF">PGT21_034952</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="11"/>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGA5"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF101">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1087644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="143" mass="15954" checksum="D3C9EB59311FA8E0" modified="2019-11-13" version="1">MTRFNLIGSLRTSVICVLFFSSVVYTLSSGVQKPGLVQSSLAKERQNEVKKVFASAYADYMKFGFPFDEVRPVAKRGQNTRNGWSATLVDSLDTLFIMGLKNEFDQGVSHTLKIDFSKSQTNDSVSLFETTIRYLGGMLSVML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHQ9</accession>
    <name>A0A5B0NHQ9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003844</name>
        <name evidence="1" type="ORF">PGTUg99_030092</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099:SF7">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1088263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="753" mass="85405" checksum="4635FD11F94060FA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJY7</accession>
    <name>A0A5B0NJY7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2503447at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="135"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1089567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="231" mass="24917" checksum="F8ABE7D27E3F2199" modified="2019-11-13" version="1">MLSKPYPPSTAKYPTKIPQASTNKQLSHVAREGSVITVAGQGRVSDQDKHDELKSKPELYAQDVALRGEDATKDELGCIGSDNDATLRGDYEIEDKDIGLLGSNGEMFSRKDATDNKAVRLIGSNGEVTLGSQDTVDNDDDEMESNTSSHGREAEDSTNNEATGSLGSNREVTLGSQDTVETDDNDMESNESVHGGEEEGSVGSEGALLWEARILSQPKMKILKKVVKRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NMT5</accession>
    <name>A0A5B0NMT5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_008693</name>
        <name evidence="4" type="ORF">PGTUg99_024222</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NMT5"/>
    <dbReference type="OrthoDB" id="2152896at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="492"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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            <begin position="10"/>
            <end position="20"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
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            <end position="118"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1074142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1090645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="562" mass="61766" checksum="B074637750655627" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NNT0</accession>
    <name>A0A5B0NNT0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NNT0"/>
    <dbReference type="OMA" id="HSMATPI"/>
    <dbReference type="OrthoDB" id="2503200at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="246" mass="27086" checksum="FBA478EC8587392B" modified="2019-11-13" version="1">MSHSQSTSTKACPLLSVVTSTNMDQLPPASEAPNAEASSPRRKLPLGAIVSPLSPFFNERAKNCALEQAKSKALETALSGLDVASESKNRSRSTSQSSKLSEASSTGSLSSSSSSHDSQHSAASPISMLDNETLLPSLPRISYEDWSKFGINLFTPKLLNPHSKMYYHYPSIEKKKNNLNHLTGNRPRSKSSTVHGYSPPQFEDHRINRPGIHQLSNPALQSRPHPQSPLTRFMSLRHPLERNYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NP89</accession>
    <name>A0A5B0NP89_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NP89"/>
    <dbReference type="OrthoDB" id="2150628at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051667">
        <property type="entry name" value="Archaeal_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096">
        <property type="entry name" value="YALI0E33429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="47"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1089758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="406" mass="46320" checksum="F8CE7A447D4C76BA" modified="2019-11-13" version="1">MISYFCCRNTLRPLKQDTASFLHQTFARNFYNRSLEVEHLTTQLKAPPQFTVLLGPPSSGKTALARHVATQNRLNNTPEFHPLIIDLREVDTSEKGSFLNAFIDDGLIRTAVRNTFWGFMSNVAEVAALGVSANLRDSHPSPQKAATVFRELGSRLKPFSLSHHGERPPVLIIDEANAFKQIDDEATGAFLSFAVRITKQESKMHVIFTSSDSFFENWLKKRINPTHFNTLVVGDLPREEAYKYLLHLVETHPYLSSQNKNRLKSVNFDVPFRITGGRMFFIKQYVEQVHMSGYFEDPMDFEPTLTSCATLEDDFRGKAKTYKTKEALRVCQLLLNSNGYISFAGLTAELSNTVVEEMIERNFLHYQLLSKFSRDLIPPPMESVLTAQSEPARRAMEALSKRYEQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NRF9</accession>
    <name>A0A5B0NRF9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamate-rich WD repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RRB1_1</name>
        <name evidence="7" type="ORF">PGT21_027754</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NRF9"/>
    <dbReference type="OrthoDB" id="2161379at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051972">
        <property type="entry name" value="Glutamate-rich_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903:SF1">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="6">
        <location>
            <begin position="142"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="373"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="419"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="470"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="59"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00040876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1091176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="568" mass="62834" checksum="63DA346E7C038316" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NXB1</accession>
    <name>A0A5B0NXB1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridylate synthase 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA-uridine isomerase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PUS1_1</name>
        <name evidence="10" type="ORF">PGT21_002036</name>
        <name evidence="11" type="ORF">PGTUg99_008981</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="11 13">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000115">
        <property type="protein sequence ID" value="KAA1129995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NXB1"/>
    <dbReference type="OrthoDB" id="10256309at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02568">
        <property type="entry name" value="PseudoU_synth_PUS1_PUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000015">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041708">
        <property type="entry name" value="PUS1/PUS2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF4">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="9">
        <location>
            <begin position="284"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="499"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="507"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="528"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00079074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00080857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1093891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1129995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="583" mass="66896" checksum="8426399E49CA5B5C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NY12</accession>
    <name>A0A5B0NY12_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019921</name>
        <name evidence="3" type="ORF">PGTUg99_025902</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NY12"/>
    <dbReference type="OrthoDB" id="2495482at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137592" evidence="1">
        <location>
            <begin position="23"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1115691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="213" mass="24197" checksum="71DE29D15C3413F2" modified="2019-11-13" version="1">MQLPSGLAFITLLVSFPLTMRAQNVPNDYIIKERPESETLRAHREVYSADGKVAYTFRKQINFPSQYKNMATIADSSLKPIFSMVSSDDQCRLKTHYAQLDGPAAKDTLLMREYKHDNSRAFGKKLWRFNFYSDAVGARVYYKFEQNHTNKGGRIYKVAAGQASQLVGLLRSQLRRDNWLDPVNGTKTFTLSCIDGAPMPELVALLGLVFFHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NY72</accession>
    <name>A0A5B0NY72_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ADP-ribosylation factor, Arf Arf6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ARF6_2</name>
        <name evidence="7" type="ORF">PGT21_022352</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NY72"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000412">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1093040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="149" mass="16605" checksum="2E49FDDD52E4FA12" modified="2019-11-13" version="1">MGGGLSKALGKLLGNKEMRILMLGLDAAGKTTILYKLKLNQSVTTIPTVGFNVETVTYKNVKFNVWDVGGQDKIRPLWRHYYTGTQGLVFVVDSQDRERIEEARQELHRILSDREMKDCLLLVFANKQDLPGAIASSTIAMQIPVRALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZ44</accession>
    <name>A0A5B0NZ44_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_022736</name>
        <name evidence="4" type="ORF">PGTUg99_015054</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZ44"/>
    <dbReference type="OrthoDB" id="2507046at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137610" description="hAT-like transposase RNase-H fold domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1115624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="28335" checksum="85728E6C45F4F475" modified="2019-11-13" version="1">MHIRCFCHKLALIVNAGLQALALKTLPPGKTKHSVLGFFPFLGKLAELPEEDKNHDLGIDVQEITVASQPEENPDEIKDKQESDYGNADDEESSEGTESDDNPDAEPRNGQKSGEGQMAKDKHSRSAKLRDLTLQLDTVIRQITQSSATRGLFQQTADALGIRCSPLIAGYGIRWNIKYESHKRAILAREVIDKILKDDQESVEKSQRKLRNKNNSATDPNIGIFNDVSFSPVDWQDIEELNSELKVISPTLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P0C9</accession>
    <name>A0A5B0P0C9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_019137</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P0C9"/>
    <dbReference type="OrthoDB" id="2505291at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="331"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1094362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="491" mass="56058" checksum="1A40A7EFA1DF9611" modified="2019-11-13" version="1">MGLGKSLQAICLIACTLDEAKEYSSNTTKSSEEMNKSHATLIICPARLLTNWSQEIQKHVVENTMKFSKYHGTQRHSTVNELISSADVIITTYEIVRAEFQECKNHSPQNKSLIFSQLWFRIILDEAHIIRTTDSKTQSAIQSIKSERRLCLTGTPLQNSLHDVMALLNFICSNLKTNHNNWVQIVSPYLKNGNVKPLQLILRHVMLRRMKNIALPNLPEITHQIIKTPLTGPARKYYDEYFEKFLKSYKSQSSSTSKKEKQIFSHLNCLRGICDHPLLADPDLDLSDDQATFRINQSQSNPNSGKSERTITHEVSHTMCCQSQKIIHLCQMLKHGSKLMEKNTKVIVFSTWTRFLDLIGIALNQHQIKFFRLDGSLDSRSQDKCLKDFSISLNTSVLLASLQTAGVGLNITCASVAFIMEPHWNPTIEAQAVDRLHRIGQERKVQVFHFITPDTIEQKIQSIQNTKKQLAQSTILPTMDWRELLQEIISM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2W0</accession>
    <name>A0A5B0P2W0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_019941</name>
        <name evidence="3" type="ORF">PGTUg99_009395</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000479">
        <property type="protein sequence ID" value="KAA1071175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2W0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474023" evidence="2">
        <location>
            <begin position="23"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1071175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1094389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="199" mass="22708" checksum="10202F9A989D250A" modified="2019-11-13" version="1">MLAPLAKLPLMVLLLATKLSKTNEHLLHVDMPKPEKYQTTILMQNTPVFQSDPNPSGDRTTTLLEKLERVTGLDKNAIRETERFIGSIPELKRQAIRLETLGIDDIDSLPDPLISMQEEYDSIRQDISQIIQGSRLGSAYNNVQHLEYFENNLKLIHTLKRRFIFGSKKKNEYGEEPSKNGSSINGKTIENLESMMKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4Q7</accession>
    <name>A0A5B0P4Q7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4Q7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="66" mass="7458" checksum="E7FC08E442A1AF90" modified="2019-11-13" version="1">MDGDVEAIGTQPTIQQRPAAPVGSLLKLNEDIYNLNHQDVEQVSGCYLSDLQARQRYFYLSMDLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P512</accession>
    <name>A0A5B0P512_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxin-7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PEX7_2</name>
        <name evidence="12" type="ORF">PGT21_034962</name>
        <name evidence="13" type="ORF">PGTUg99_004253</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="13 15">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat peroxin-7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1095049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000238">
        <property type="protein sequence ID" value="KAA1121315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P512"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005053">
        <property type="term" value="F:peroxisome matrix targeting signal-2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044536">
        <property type="entry name" value="PEX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027:SF1">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="60"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="104"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="147"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="193"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="243"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1095049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1121315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="333" mass="36742" checksum="6D788435B146A9D5" modified="2019-11-13" version="1">MSQLIQRCGTPGFAGYSCAYSPFYPNKLAVATAANFGLVGNGRLHILQADPSTSLNIEKLFDSQDGLYDVAWSEIHENQLATASGDGSIKLWDIMLNEFPMQSWHEHKREVFCLDWNNLKKEIFASSSWDHLVKIWTPSRSDSIMTIPAHESCVYAARFSPSSPDTLATCSSDGSLKIWDTRNSPETRPPLVIPAHSNEVLSLDWNKYATHFIATASVDRTVKIHDIRKASAGSAHNSCVETLVGHQYAIRKVAWSPHAADKIATCGYDMTARVWMIPQISGQAPLPGAMGAISPLGIHNAHKEFVVGLAWSLYHPGLLATASWDQEVHLWSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P592</accession>
    <name>A0A5B0P592_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COG complex component COG2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_016649</name>
        <name evidence="2" type="ORF">PGTUg99_022490</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000372">
        <property type="protein sequence ID" value="KAA1095328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P592"/>
    <dbReference type="OrthoDB" id="332281at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009316">
        <property type="entry name" value="COG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024603">
        <property type="entry name" value="COG_complex_COG2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12022">
        <property type="entry name" value="COG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COG complex component COG2 C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="134" mass="15202" checksum="FE928CDA299E6CE2" modified="2019-11-13" version="1">MIPTKPSYFISDILNDIQVYLEKYGKNLTEQVRTDLVSGIIDELSAKYLAILINVQRSEDSLRKLKKGKHGFSIFNRNSNSDSNKASPLVEDDELKVKVQLRLDVERLELDSIRLGADLSSSKSFLELKQTVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P5S0</accession>
    <name>A0A5B0P5S0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Small EDRK-rich factor-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_000914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000072">
        <property type="protein sequence ID" value="KAA1095548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P5S0"/>
    <dbReference type="OrthoDB" id="370932at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1050.10">
        <property type="entry name" value="At2g23090-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039713">
        <property type="entry name" value="At2g23090-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007513">
        <property type="entry name" value="SERF-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026939">
        <property type="entry name" value="ZNF706/At2g23090_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33788">
        <property type="entry name" value="OS07G0114300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33788:SF1">
        <property type="entry name" value="ZINC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04419">
        <property type="entry name" value="SERF-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118359">
        <property type="entry name" value="Expressed protein At2g23090/F21P24.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small EDRK-rich factor-like N-terminal" evidence="2">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1095548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="146" mass="15700" checksum="B1F73637E57A7CFA" modified="2019-11-13" version="1">MSTHMPGKILQPSKVGGGKAAGILKFAPHTKPPRNFIPLPPYSTSTVHKLRTTHQQTTSPISVCVQHSDISHQPLDSSYPLSKPSSHIFDRTMGGGNGAKAAQKRERNAKASGATAKSQLKVNEAAKNIQCKVCFQTFLITSREPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PBY3</accession>
    <name>A0A5B0PBY3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003664</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PBY3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903:SF1">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022737515" evidence="2">
        <location>
            <begin position="19"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1097349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="343" mass="34826" checksum="6C4FA641A1CC7160" modified="2019-11-13" version="1">MLHKTQLLSLLLASTALCRSLGNLNQGMLHERSLPAMEDGQHSLLEIHLLILFLTFHHPQKRRLIKREDLAPGIQAPDLQPKDVSLGPEKPPKKEKKEKKEKKEKEKEEKPDPSLQPNGGDTPGDTNAHEQDTGGDADPKGKDKDKGKSAKKPSAEDKEKKKQDKIEKQKKKVQKAQDKLDAEKAKLEKLQNGGDDKGKGEGKEKKGKKEKKGSKSKEENPNGGDQQPKDLDPTPDPSDNGLGGGSGNGSGGGGSGSGGGGSGSGAGGSGSGAGGSGSGGGGGSGGGSGGGNGGKKGGEKGGDKGKGSTTTPGDVVGGGKGSGSGSGNNNPKDAGQEDPNPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PCG0</accession>
    <name>A0A5B0PCG0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000897</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PCG0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="164" mass="18308" checksum="809AA4F9D314894F" modified="2019-11-13" version="1">MLTNFHRLYSSFRDRRPYKQVRLGLLSVSFTLLATNIFRVMTNSSSWACLIDFISDFAIGAGPIAGAFFTAQERAASQVEFITLHFSSSFGGIDIHPPIETQFKRLTSGFRDRQGTSVKAARPKIQVEIKSKREVFCQSKVTAIDAEPLTEGQATTVTYRSSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PK96</accession>
    <name>A0A5B0PK96_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036098</name>
        <name evidence="2" type="ORF">PGTUg99_021355</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PK96"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1098511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1100349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="53" mass="5926" checksum="293232D740D969F1" modified="2019-11-13" version="1">MFTRKSNLSSVDVLFMMRGFSGAPFSRGPFFFSAIIITDDSAPTRFSIISSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQ51</accession>
    <name>A0A5B0PQ51_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 71 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQ51"/>
    <dbReference type="OrthoDB" id="3257981at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051118">
        <property type="term" value="F:glucan endo-1,3-alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11577">
        <property type="entry name" value="GH71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005197">
        <property type="entry name" value="Glyco_hydro_71"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03659">
        <property type="entry name" value="Glyco_hydro_71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="54458" checksum="7AFFB1E57FD7AD9A" modified="2019-11-13" version="1">MGIVLTFTIMGTRQSRGKGSVAEGSQAINADEVIIRVIPDPRDSHPPGPQHHPEPAIPNERPRYVFAHVVQGNFQFYQAEDWASDMRLAQRMGIDAFAINIGRDVTNEKQLPLIYEIAEMNSFQVFLSFDMVYYGQAGSSNDVIRLIETFAPRKAQFMYQGKPLVSTFSGEVPGNYLDNNPDYNSAWGSLKAKLHFPIYFMPCWTGIDPKTVSSIDGMLSWDAWSPVSSSQDRRYKDNLAALGKQYAAPLSAMFYKHLSAQRYGNYLYPTESWFVVEKFIALISLNPDFIEILSWNDYGESHYLRDPRPSANLPMDTTSSEKYVNHMPHEPLLDLISYFNEWYKSGSRPLIKRSRAYVWYRTHPRDAVSKSDLLPAPNGANVTEDKMYIVILVSSATKLQYISIESGDKYHYTDLEEHETFKRKDAILLISVPFHVGDNQTVTLYDPDETALGCLVGRGITAEPEIYNFNYWSGFIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQI3</accession>
    <name>A0A5B0PQI3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glucosyltransferase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ALG6_10</name>
        <name evidence="4" type="synonym">ALG6_1</name>
        <name evidence="2" type="synonym">ALG6_2</name>
        <name evidence="3" type="ORF">PGT21_036898</name>
        <name evidence="4" type="ORF">PGTUg99_002170</name>
        <name evidence="2" type="ORF">PGTUg99_002181</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000392">
        <property type="protein sequence ID" value="KAA1090306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000321">
        <property type="protein sequence ID" value="KAA1103648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQI3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1102219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1103648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="111" mass="11848" checksum="35887070694B503F" modified="2019-11-13" version="1">MLKVGPLPQQMLKEQAGSGNRASSQVMISLPSGEKLMNRIKNPLHVAASRSASPSSSSSASHRSSSLKDRRRTSSSSKGKEKEDDHELDNQGGNQADGGHLNHSSRGTGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PXC1</accession>
    <name>A0A5B0PXC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_011595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PXC1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="144" mass="16308" checksum="28017B1B9FA1C686" modified="2019-11-13" version="1">MRAEKLFFWNLSSGSLHDHNKGRIVLHILRRFRHHEPRNFGLKGGGTGLGSPSSESFGQSIALVKTGPSTVLHDFFVCQWATCADRRRLGLTGWKRKPAPLHCYHELSLWLITHRRKESIFQRPAMVEPGTMSTGLSECILTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYC9</accession>
    <name>A0A5B0PYC9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cu/Pi carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PIC2_2</name>
        <name evidence="13" type="ORF">PGT21_023734</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transport of phosphate groups from the cytosol to the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYC9"/>
    <dbReference type="OrthoDB" id="2503921at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035434">
        <property type="term" value="P:copper ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000076">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000131">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="77"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="174"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="275"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1105906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="372" mass="39877" checksum="54684D23E60BE0D9" modified="2019-11-13" version="1">MQSILLPHLVSPFNFNSTLGDFNTPLHPWKAAGILDGSKSKAKQIEEGIDRRSKASIVSSSGIGADSTASPQLHSTRYYTACISAGVAACGLTHALVTPLDLVKCRRQVDKTLYKGNLDGWIKIYKAGGVAGLYTGVGPTWLGYSVQGGCKYGFYEYFKKKYADAVGPRNAVKYKDGIYLAGSASAELIADAAYVPLEAVKVRMQTTIPPFAAGTVDGFQKFVAVEGVQGLYKSLGSLWSRQIPYTMMKFWSFEATVSKIYASLGKPKESYNKLQQLGVSFVGGYFAGIFCAVVSHPADTMVSKLNAVGKGGSGKPTVSSIYKEIGFGGLWAGLGTRIVMVGTLTALQWLIYDYVKVAFGFPTTGAAQPQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYU5</accession>
    <name>A0A5B0PYU5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_024514</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYU5"/>
    <dbReference type="OrthoDB" id="2689033at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1105939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="643" mass="73680" checksum="4C72CA8E2E06EC1C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q050</accession>
    <name>A0A5B0Q050_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_036184</name>
        <name evidence="5" type="ORF">PGTUg99_019053</name>
        <name evidence="4" type="ORF">PGTUg99_035138</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000237">
        <property type="protein sequence ID" value="KAA1121899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q050"/>
    <dbReference type="OrthoDB" id="341300at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1106560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1121899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1126244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="302" mass="34762" checksum="AC11C1A922720ACE" modified="2019-11-13" version="1">MVLSHLDELVIPHPFRFVCQAFTLRYPNPFAPHVITADTLERSWDAESGTLRSTRLVLKRGTLPSWAPRGIVEKSETWVLEESTINILDGTMTLSNRNLDHRRVMEVIERITLRALGEVTEATSSVKVTSSWGFYAIRKRIEQYGISRFQRQSRNSRHGLELIIKLLRPSSPLREPLLNAERINYPPSLAPTSPLKSRLRELRLRAKEKQRLISEMLRPANKKESSPSEHESDEDEDHEDDSLTAAEEAELDQLECEFSADPSSSKLASPLSNPSPQKRRWFWWWRWHLSSDTAPEDKSSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q207</accession>
    <name>A0A5B0Q207_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q207"/>
    <dbReference type="OrthoDB" id="2504836at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="134" mass="14792" checksum="B85896D80C142ECA" modified="2019-11-13" version="1">MVEEAPAQPSRNEEVQKASELSSSSLMKKVKAKIRAGPKNSDMPASPSETTRPERMEPELPPRARRTVDRFCQAHGFSNPLQVDPNVPGKKHSGYPKKNSGETDGLGAAITSSERKYFKWPLQEVGSRLQASMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q727</accession>
    <name>A0A5B0Q727_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q727"/>
    <dbReference type="OMA" id="SHVRRGH"/>
    <dbReference type="OrthoDB" id="2117453at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1109056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="16875" checksum="3A7415567B3E76D5" modified="2019-11-13" version="1">MTFARAAHVAVFSLIIIGCIAEIVTSGILVGNFNNLKDAELFTSIVTRTKYLLFCGLWTILFSTIYVVGLATAPGHLLFSVASHLIWLGVTIVLFVSGSAALQTEINAHIFPHQQRLEILQGFAWADSILLGVAMLIVLVIGIGRRNGLSGNLLGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAT0</accession>
    <name>A0A5B0QAT0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_018238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAT0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1110328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="137" mass="15546" checksum="00525D77C449D73A" modified="2019-11-13" version="1">MKFFTPAQHLTFSSPNIRPLGGPRGGFCFALYNWPWASAKLGFIKLVIKGISNMPIHYVVDIVKGTDLHIESSPEVDQRRGWMVRVEFWIKKVSLWGCIQPTFNCFILHSPRIHGEASPALLVSIRDGLWLQVKELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QCI9</accession>
    <name>A0A5B0QCI9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">RP/EB family microtubule-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGT21_032691</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QCI9"/>
    <dbReference type="OMA" id="WIKRFWD"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035372">
        <property type="term" value="P:protein localization to microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000007">
        <property type="entry name" value="Protein BIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000028">
        <property type="entry name" value="RP/EB family microtubule-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="12">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="13">
        <location>
            <begin position="163"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1110839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="28623" checksum="5F08BB8EA1F7068D" modified="2019-11-13" version="1">MTSRAELIAWVNDLLQFNYTKIEQCGSGAAYAQIIDSIYQDVPLARIKMNANQEYEYLNNFKVIQAAFKKHQIDKPVPVTALIKCKMQDNLEFLQWLKKYWDMHYPGGQYDAVARRKGAPADPNPLIAAAAPVKTSSNGAARTGKTLGMSTGMGARATSRTGNTLGTQKEIENLTTQLEEFKLSMEGLEKERDFYFNKLRDIEILIGARVDSKDGNVSETEMENLKQMQAILYSTEEGFEVPEVDAGLVDEEETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QF52</accession>
    <name>A0A5B0QF52_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zinc-ribbon 15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012982</name>
        <name evidence="1" type="ORF">PGT21_015181</name>
        <name evidence="2" type="ORF">PGTUg99_026549</name>
        <name evidence="4" type="ORF">PGTUg99_030402</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000374">
        <property type="protein sequence ID" value="KAA1093543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000136">
        <property type="protein sequence ID" value="KAA1129576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QF52"/>
    <dbReference type="OMA" id="TICRWEM"/>
    <dbReference type="OrthoDB" id="5545479at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139:SF1">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1067714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1111818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1129576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11571" checksum="1D328B32610128A4" modified="2019-11-13" version="1">MCFFIPLQFGCPTKIAQIDAEKDDIRICPQCHNAAVTSCKTRMWFEFCFLPIIPFKSKKVWLCSICRWQALNDGTGPQALSNIPPPGGGYAAQAPMAAPIAHTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFJ1</accession>
    <name>A0A5B0QFJ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFJ1"/>
    <dbReference type="OrthoDB" id="10294243at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="182" mass="20775" checksum="20F0ABB0C268B6FE" modified="2019-11-13" version="1">MTTLVRKEATTATAEKSKKNRDVLPPSTEKETPMDLDELESESSEVTQIRPSTPEIRILSKEEQIKLRVKEHVALWRKCQVATCEGATPKLRALLTEAQESQKTLQKLIDNEEIESYVKGWNPWDEKKIHFPAPPKKQVMKFKRSEGGKRQSSSSNNFADPAKWKRATELLQIAAGLYQNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QG04</accession>
    <name>A0A5B0QG04_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome assembly chaperone 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QG04"/>
    <dbReference type="OrthoDB" id="2499509at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="37152" checksum="67C3301A21B393C1" modified="2019-11-13" version="1">MSAQILDFHHSTGPAPAYAIESDSEEDEWADEPRQDAEEGRSGTKPSGEGVERPLEWQPTVPERLEFPGESGLLVLVGDAGLLVGSGLPEHLRSPSQSTIQLDQSLLLFQPARFALTPRHVPLHLQSALAHKLLADIRPPSLTIISTYQAPTYIPAAEHFPIRYLASSVSDPSSTLPHSLETMGCQHFQVPNLIKGFEAALMIQANILQIDSKVILLPAISIPHDDKSSTSIPVGQYDFSSASTGIDGPESAGLPASLLGLDDPAVRRTLGAVIEDVHLKHLRPTHGMDQHVTQAHWRFPLALEPALKDAFRKQLNLDQQLKPKPHPSAMRDHEIGLMYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QI97</accession>
    <name>A0A5B0QI97_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QI97"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="172" mass="19866" checksum="348E8EA9D7BC55DE" modified="2019-11-13" version="1">MSRGQSRERRRDGRTVYRCSLCDGAEVLSYRSHALGTQHRENVREYDEQMARAQRERIEEEREETNPQQEVQDNMDNEEDEDTYDIQADLDAMGAAARFLLPDESSDINLDDEIGLDDLIDAELENILRNEDTVTFDGPADEDDRDNDPVENSRWFPFRNKMVSSLSSPGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIP6</accession>
    <name>A0A5B0QIP6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA decay factor PAT1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_017306</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIP6"/>
    <dbReference type="OrthoDB" id="74835at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039900">
        <property type="entry name" value="Pat1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019167">
        <property type="entry name" value="PAT1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551:SF0">
        <property type="entry name" value="PROTEIN ASSOCIATED WITH TOPO II RELATED-1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551">
        <property type="entry name" value="TOPOISOMERASE II-ASSOCIATED PROTEIN PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09770">
        <property type="entry name" value="PAT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="36"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="860"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="99"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="329"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="549"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1112972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="949" mass="104639" checksum="70279632CDE32AF5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QL51</accession>
    <name>A0A5B0QL51_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">chitin deacetylase</fullName>
            <ecNumber evidence="20">3.5.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PGT21_030011</name>
        <name evidence="26" type="ORF">PGTUg99_020375</name>
    </gene>
    <organism evidence="26 28">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">21-0</strain>
            <strain evidence="26 28">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + n H2O = chitosan + n acetate</text>
            <dbReference type="Rhea" id="RHEA:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9597"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57704"/>
            <dbReference type="EC" id="3.5.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:10465"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the polysaccharide deacetylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.41" evidence="20"/>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000274">
        <property type="protein sequence ID" value="KAA1113938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QL51"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004099">
        <property type="term" value="F:chitin deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.370:FF:000004">
        <property type="entry name" value="Related to Chitin deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050248">
        <property type="entry name" value="Polysacc_deacetylase_ArnD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587:SF98">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="18" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="16" id="KW-0170">Cobalt</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474602" description="chitin deacetylase" evidence="23">
        <location>
            <begin position="19"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="24">
        <location>
            <begin position="144"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="367"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="379"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00024056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAA1078174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAA1113938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="432" mass="46932" checksum="19F76404CB93EDB4" modified="2019-11-13" version="1">MLLIPIFLAIISLTSVSACGHDLHLYRRQRSTTWQSAAQSTDPTVECAPYSYAPVQAVVKTYPAIWQIANLSQPGLDPQALQVYSQIQSKIPNIAVKGTPTGDFTATTPNYPRSDPDCWWSFGKCTRPKLAGLSPDISTCPEPNTFGFTLDDGPNCSHNAYYDLLRQTNQKASLFYVGSNVIDWPLEAQRGLADGHEICSHTWSHKYMTGLTNEQAFAELYFSKKIIKDVLGITVQCWRPPFGDTDDRIRFIAASLGLSTVLWDNDVNDFRFATLGQAAVNANYQNLLDGSARGDFNTHGTIVLSHELNGGTMNTSAAYLPKIQQAFKHVVPVAVCQNISQPYLETGYNYPSFAEWVKGTRVKNIPDPVSNPGAPLQIPSSPAQGSSSYPSAGANTGSGGAAQKNGAASAYRTSIERLSILGLLTLTIVGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQH0</accession>
    <name>A0A5B0QQH0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="16">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PGT21_036917</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Protein phosphatase that specifically binds to and dephosphorylates the molecular chaperone Hsp90. Dephosphorylation positively regulates the Hsp90 chaperone machinery.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="16"/>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQH0"/>
    <dbReference type="OrthoDB" id="445564at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000595">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000039">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013235">
        <property type="entry name" value="PPP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08321">
        <property type="entry name" value="PPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033096">
        <property type="entry name" value="PPPtase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="18">
        <location>
            <begin position="358"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR033096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1115501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="583" mass="64536" checksum="500018FCF5E6CC1D" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QRF5</accession>
    <name>A0A5B0QRF5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-complex protein 1 subunit zeta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCT-zeta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CCT6_2</name>
        <name evidence="10" type="ORF">PGT21_036487</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QRF5"/>
    <dbReference type="OrthoDB" id="10052040at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03342">
        <property type="entry name" value="TCP1_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000038">
        <property type="entry name" value="Chaperonin containing TCP1 subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000058">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000004">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012722">
        <property type="entry name" value="Chap_CCT_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02347">
        <property type="entry name" value="chap_CCT_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1115444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="554" mass="60382" checksum="DD682BEA2F9E74F7" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVA0</accession>
    <name>A0A5B0QVA0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">NineTeen Complex (NTC) component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CLF1_2</name>
        <name evidence="13" type="ORF">PGT21_030089</name>
        <name evidence="14" type="ORF">PGTUg99_026336</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
            <strain evidence="14 16">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in pre-mRNA splicing and cell cycle progression. Required for the spliceosome assembly and initiation of the DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVA0"/>
    <dbReference type="OrthoDB" id="541719at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000048">
        <property type="entry name" value="Cell cycle control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000491">
        <property type="entry name" value="Crooked neck-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF3">
        <property type="entry name" value="CROOKED NECK-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="10" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="12">
        <location>
            <begin position="59"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="71"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="12">
        <location>
            <begin position="311"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="712"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="745"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1098189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1116845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="754" mass="89871" checksum="5A7A60A4444324D9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVN2</accession>
    <name>A0A5B0QVN2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">T6SS Phospholipase effector Tle1-like catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVN2"/>
    <dbReference type="OrthoDB" id="3057168at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018712">
        <property type="entry name" value="Tle1-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840:SF1">
        <property type="entry name" value="TLE1 PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09994">
        <property type="entry name" value="T6SS_Tle1-like_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T6SS Phospholipase effector Tle1-like catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="375" mass="43257" checksum="5838A3CCC85AA87D" modified="2019-11-13" version="1">MVAEIGILNMAGMEDFYQVLAACQLLHAKGDSDRVERGRASAQAFLDEYYQGGQKQLRRISEGSLKCVGVWDMVGACGLPEPFAQKYPLLGFGDRKLSVHIKYAFHAIAMSETRMDLQPTKWELGSTSKECNEAEQKQVLKQVWFPCSHIEAGGRIEEYDDVTYISLLWMAATLIHHNLLAINEEYLHGMLSVFWTRVQRGFGLYDPEGPRRYCLSSTTREIPKSWNALTKETIHRSAFSSPERMIPGLEQVQTDCKAEPELLEELLPFEIEIRNHLDFLTKQWAVHEGHRVRPDEFNDWPDQQSWCQERWYLNFKDTQLRSKPQTHGLFLDLMDQPFWPLDTRRLKEERVSRGRGRGHASVEDAAMCMLGTPMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QX81</accession>
    <name>A0A5B0QX81_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_027353</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QX81"/>
    <dbReference type="OrthoDB" id="5370059at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="264"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="7"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="478" mass="52373" checksum="D8E692C27B2C775B" modified="2019-11-13" version="1">MAQLLLYASGSNSHGQLGTRNLKEEEEKEEDLHEFTNTHQWNHHHQQPQGEEQQQQPISSSIGGRHSLFLINHPTRPEIYLCGDQNQGQSGPKISSIAPSPSIGENAPSIINQSSQPYDTNDECSSTMKMIDYLKLISMTSNIDHELKELLGQHYRPVQVTACWETSFVVLSPRFCGPSTKDETRMEEEEIQYDDEILAFGSNDFDLRGSAEKETLPEDPGTPNLVDLPPSVGRTGSGRRRIRLHGGCQHVIAVIEYAGQGGPIELVGWGAARHGQLGPLETAGGKPVRTSLPTRLPISIPLGTPLSALKIALGNQHSVLVSPTQIWCWGSNRNGQIPSALKALPPADILELQATWNATFVILSLPDRPTFKRLLGYGSNSHGQLLGTSHLHLDRLIPSHHANLVAGSEHLLLTSTLQNNTVQIWGWGWNEHGNLGKREGHPLEPIRAFSLLFEIPSNSKLVALAAGCATSFVIVCSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QY86</accession>
    <name>A0A5B0QY86_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QY86"/>
    <dbReference type="OrthoDB" id="10276119at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023033522" evidence="1">
        <location>
            <begin position="24"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="241" mass="25987" checksum="DD6B1B3245A67682" modified="2019-11-13" version="1">MASFKLFLVAMSLLANHISLSDAAQCNDGFNLDLKECKVARDSIAYVPGKDIIKSIERAIQVSSGNCMIQIDVVPTPSPVISKKEIDETINDILSCATHAGINDPVKNIQVQISSIPSSYGATYGPNQKMDTAVCNTDADGGKNEQQDCLKAFNKISTNKQGQLLDENGRATYKISRKEKSCIVQISSTNGLPVTEKLDKLSKSLNIILNTCKKQSGYLARLRGTDGLNGRLSVKTLSAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZ50</accession>
    <name>A0A5B0QZ50_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_031421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1118105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZ50"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1118105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="8451" checksum="5804D92616F75D05" modified="2019-11-13" version="1">MALRLMRTAFDRRRSDPLPYLCRRLTRMRAIDRQNDPQLDANLANALVDRHRDHHRYCQPESQWWCLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0B7</accession>
    <name>A0A5B0R0B7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-mRNA processing factor 4 (PRP4)-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_012193</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0B7"/>
    <dbReference type="OrthoDB" id="540662at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001211">
        <property type="entry name" value="CBN-PRP-4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000443">
        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein Prp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.110">
        <property type="entry name" value="Pre-mRNA processing factor 4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036285">
        <property type="entry name" value="PRP4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158230">
        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="5">
        <location>
            <begin position="47"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="180"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="229"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="279"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="321"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="363"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1118988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="540" mass="59563" checksum="97DBA6A8F75196A6" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R1Z9</accession>
    <name>A0A5B0R1Z9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">54S ribosomal protein L22, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_029803</name>
        <name evidence="6" type="ORF">PGTUg99_035605</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000304">
        <property type="protein sequence ID" value="KAA1109834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R1Z9"/>
    <dbReference type="OMA" id="PAEHEDT"/>
    <dbReference type="OrthoDB" id="416470at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000037">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047867">
        <property type="entry name" value="Ribosomal_uL22_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="CHLOROPLAST 50S RIBOSOMAL PROTEIN L22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL22M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="143"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1109834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1119592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="38293" checksum="D585ACBE6E8903D4" modified="2019-11-13" version="1">MKAILNPRNLQTTVKFTSTIQRPFSISSNQYQDEQVKAKGITPQKISILKRQAVANAEASYESLPPMTSGFDQLSIKEPKSFLRPRIHLSSLPKLALKLTRPAKETEAYGINFGVLPRSQPMPQERTVPEQKPVSLIDQALRSRPTEPQLAEYQDSISADPSEETKARKTEFGTSNNDPSCHNASSDLLVVSHKKLQKLADLINRHRMSVDQALIQMRFSHKAIAAKVLDLLLKAKQEAIERGFKPDRLVIAEAWVTKGFHTSELQVRARGRHGKISHPTAKFNVVLRQSTDANKLALQRRLALKESNRRLTQRGTIAGSPIRVPADGVLPSSVAHRPFWGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R481</accession>
    <name>A0A5B0R481_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R481"/>
    <dbReference type="OrthoDB" id="284184at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF33">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14860)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="46"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="137" mass="15319" checksum="7B4C19B0374B1ABD" modified="2019-11-13" version="1">MSGNQTPNPKDPGSFTRKSLVVSSGHRYSYIDQSLNADAQVVLLLHGFSKPTDLKAYSKLNSCKALCEILDHENIRKKVILVGHDWGSALAFRFVQHFPEKVKCWVTICVPPARPGQRGEAPPDFEKIIKQRLPHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R546</accession>
    <name>A0A5B0R546_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_037148</name>
        <name evidence="1" type="ORF">PGTUg99_027293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="1 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000273">
        <property type="protein sequence ID" value="KAA1114182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R546"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1114182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1120095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="174" mass="18447" checksum="B5514DF51859C2AF" modified="2019-11-13" version="1">MALRRLDPAVKHRSALTSFTQSRWIALANVKFGQECRNSNPPQACLATAGKPTAGDYAPRYEAPQIIGRGGMGQADGTASVKHSSTRARGVVDVGDLVSPELGLSQTWASAKVRPPRDLPNQAPWGLAQAPVARGHAGLGPVPRPACNTILVERQDLLQSRHLTCSARGLALSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RLL6</accession>
    <name>A0A5B0RLL6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Store-operated calcium entry-associated regulatory factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Transmembrane protein 66</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGT21_033863</name>
        <name evidence="17" type="ORF">PGTUg99_011295</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="17 19">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SARAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000176">
        <property type="protein sequence ID" value="KAA1125614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RLL6"/>
    <dbReference type="OrthoDB" id="20303at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001256">
        <property type="term" value="P:regulation of store-operated calcium entry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009567">
        <property type="entry name" value="SARAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15929">
        <property type="entry name" value="STORE-OPERATED CALCIUM ENTRY-ASSOCIATED REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15929:SF0">
        <property type="entry name" value="STORE-OPERATED CALCIUM ENTRY-ASSOCIATED REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06682">
        <property type="entry name" value="SARAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="209"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1098345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1125614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="265" mass="28664" checksum="795E8BC5FAEA0CFA" modified="2019-11-13" version="1">MAFNSKVLLSDISTLTFFDAEQTTFRRTSPVDQLVCRGPGCKVFRPEVIQCYNKGGLGTDVNWKCEADLPAKLKLGRVEVGCEGWSNSQDPYVLKGSCALTYTLNLDTYSKYDTSAESSPAVAWVYWMVVIVIAFLFAYPYIRSFLVRFLPWLDPILADSSSGGGGGGPPPPYMPDQPPPKSAPSTSQPWWSPFLSGVVSGTVTHLLRGADTRASDRPSTGFATAPSYGRSHPTYRGQGSSSDMDDSSNLGPIQTSTGFGGTNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1W7</accession>
    <name>A0A5B0S1W7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial distribution and morphology protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mitochondrial inheritance component MDM12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MDM12_1</name>
        <name evidence="9" type="synonym">MDM12</name>
        <name evidence="13" type="synonym">MDM12_2</name>
        <name evidence="12" type="ORF">PGT21_028725</name>
        <name evidence="13" type="ORF">PGT21_029920</name>
        <name evidence="15" type="ORF">PGTUg99_029802</name>
        <name evidence="14" type="ORF">PGTUg99_030189</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
            <strain evidence="15 17">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. MDM12 is required for the interaction of the ER-resident membrane protein MMM1 and the outer mitochondrial membrane-resident beta-barrel protein MDM10. The MDM12-MMM1 subcomplex functions in the major beta-barrel assembly pathway that is responsible for biogenesis of all mitochondrial outer membrane beta-barrel proteins, and acts in a late step after the SAM complex. The MDM10-MDM12-MMM1 subcomplex further acts in the TOM40-specific pathway after the action of the MDM12-MMM1 complex. Essential for establishing and maintaining the structure of mitochondria and maintenance of mtDNA nucleoids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. A MMM1 homodimer associates with one molecule of MDM12 on each side in a pairwise head-to-tail manner, and the SMP-LTD domains of MMM1 and MDM12 generate a continuous hydrophobic tunnel for phospholipid trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion outer membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="9">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MDM12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000171">
        <property type="protein sequence ID" value="KAA1126611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1W7"/>
    <dbReference type="OMA" id="IYWERIT"/>
    <dbReference type="OrthoDB" id="3356905at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21672">
        <property type="entry name" value="SMP_Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03104">
        <property type="entry name" value="Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26544">
        <property type="entry name" value="Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1075098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1081134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1126611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1131295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="239" mass="26614" checksum="AF85F93A54217ADE" modified="2019-11-13" version="1">MSIEIDWLSLTPSIAEKLTERLNGLLANLSTPDFLGPLRIQSIDFGDIAPEVEIVDIRDVSRGFRIADGESHPSSSPECSSAPTIKPDEDGHSQPSLQIHLRLTYDGNLRLRFSTALVLNHPAAEFMSLPLRATVVGVGFDAEIILAIEGDRSKFHLSLLEPPHSELPLYEDSVENGGKRNSSIGARILPHLFVETEVGNADRHVLRNVGKVERFLTETIRSLICEEFLYPTFQTFEWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S4C0</accession>
    <name>A0A5B0S4C0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022831</name>
        <name evidence="3" type="ORF">PGTUg99_016450</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000076">
        <property type="protein sequence ID" value="KAA1132800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S4C0"/>
    <dbReference type="OrthoDB" id="10302688at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1073695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="69" mass="7682" checksum="8264C8BCFBECC8E4" modified="2019-11-13" version="1">MRLYDQPVDEDQIPSTNLLHSNWEVTEYLGTLSPQDQAHTTTAGEETTMGGHDGINEDPNTVAHVINLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S6F4</accession>
    <name>A0A5B0S6F4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028178</name>
        <name evidence="3" type="ORF">PGTUg99_019463</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000074">
        <property type="protein sequence ID" value="KAA1132995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S6F4"/>
    <dbReference type="OrthoDB" id="10503314at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="179" mass="19709" checksum="AB98B1CE8A388FD9" modified="2019-11-13" version="1">MVTISSDSRVRLNRPEGEKRPADPSLDHGSIHSFGSSNSKPKPKKKQQHKHQPASNLANTAKTSSQTKKPKAAKSSNETMDSNGKPYYYDQDSNSVWKKGDPLGTKLNFKCKWFHNTYRGQTLSNGNLKFHCDGSTQALKNPNGFPKRELSKQAGVRLPASVAELRALKMLANEGGDAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SG76</accession>
    <name>A0A5B0SG76_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002621</name>
        <name evidence="2" type="ORF">PGTUg99_010928</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000011">
        <property type="protein sequence ID" value="KAA1137176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SG76"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1096023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1137176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="97" mass="10346" checksum="2833C49AAEEBC6E2" modified="2019-11-13" version="1">MLMIGCANAAIPSSCAAVEADGTRFASSDEPLIVAFVLGVRGLSNSQLAVDRGASARPAHPWPVFHCDHLGVKLSKQLNDLYQSGCPAWPERRAKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SJ18</accession>
    <name>A0A5B0SJ18_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H3-like centromeric protein CSE4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">CENP-A homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">CENPA homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CSE4_2</name>
        <name evidence="17" type="synonym">CSE4_1</name>
        <name evidence="17" type="ORF">PGT21_003349</name>
        <name evidence="16" type="ORF">PGT21_003775</name>
        <name evidence="18" type="ORF">PGTUg99_004117</name>
        <name evidence="19" type="ORF">PGTUg99_011526</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
            <strain evidence="19 21">Ug99</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin that serves as an assembly site for the inner kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of centromeric nucleosomes, where DNA is wrapped around a histone octamer core. The octamer contains two molecules each of H2A, H2B, CSE4/CENPA and H4 assembled in one CSE4-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with the inner kinetochore.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000015">
        <property type="protein sequence ID" value="KAA1136916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000008">
        <property type="protein sequence ID" value="KAA1137419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SJ18"/>
    <dbReference type="OMA" id="SRKTICD"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000087">
        <property type="entry name" value="Probable histone 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="58"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1071333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAA1077330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAA1136916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAA1137419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="161" mass="18052" checksum="26EC48D1558B60A7" modified="2019-11-13" version="1">MAVGANKTSNQRPDKSPTNPVSKTGTKPLKKPRKSVEKKRTSDAASASKSKKPHRYRPGTVALREIRHYQKSTDLLMRKLPFARLVREIAMDYALATDDGAALRWQSSALLALQEAAEAYLVHIFEDTNLCAIHAKRVTIMKRDMELARRLRGGPWASEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LIC0</accession>
    <name>A0A5B0LIC0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000198">
        <property type="protein sequence ID" value="KAA1064091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LIC0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="514"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1064091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="660" mass="71263" checksum="B2B21A2977213E20" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LJ19</accession>
    <name>A0A5B0LJ19_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_008816</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LJ19"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1064592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="79" mass="8538" checksum="4D384BE8CD4E45E6" modified="2019-11-13" version="1">MVVAVSAHPSRSTPMLELQDPAERCSAKLSVSHIGGSCLRARPSPTDIYIWARALLFWSVHPGPDAAERSPTKALLDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LM46</accession>
    <name>A0A5B0LM46_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF6534 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_021396</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1065003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LM46"/>
    <dbReference type="OrthoDB" id="2499150at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045339">
        <property type="entry name" value="DUF6534"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20152">
        <property type="entry name" value="DUF6534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6534" evidence="3">
        <location>
            <begin position="188"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1065003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="309" mass="33498" checksum="F0F83CABFD674C99" modified="2019-11-13" version="1">MPSSSDPTFSAPGSSDPGPHMINPRTTLDSSIYVVPALTLLSCVALLQYADFCLRPASERSRQSFYLATFGISIHLVQIALDLWYMCHSFQSYLSGQGQDTMVFIYTSLEVSMAVGVAATVQLIQVMPALSRWWTIVIGFLAIASCVGGLGAGAEFGMMSHISSPTAGPPVFSIAFFAFYNMWLISNCLTDGTISVAMIIALHHSRSPIRHESLATTLRRLLFLTISTFGLTFIVALLSLIVQALPHLMPSMSIGGQEMCRRASFILNGILPRIYLNSFFCSLQCARGTKPEKLLSFTGQSPSVTGSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LRH7</accession>
    <name>A0A5B0LRH7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-galactosidase</fullName>
            <ecNumber evidence="3">3.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_023456</name>
        <name evidence="9" type="ORF">PGTUg99_018978</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000509">
        <property type="protein sequence ID" value="KAA1066739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LRH7"/>
    <dbReference type="OrthoDB" id="5795902at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000097">
        <property type="entry name" value="Alpha-galactosidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000400">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF75">
        <property type="entry name" value="ALPHA-GALACTOSIDASE MEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033844840" description="alpha-galactosidase" evidence="7">
        <location>
            <begin position="25"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha galactosidase C-terminal" evidence="8">
        <location>
            <begin position="493"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1066739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1071941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="593" mass="66392" checksum="173C29FB0C84EB3E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LU65</accession>
    <name>A0A5B0LU65_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dihydroorotase</fullName>
            <ecNumber evidence="3">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_023405</name>
        <name evidence="9" type="ORF">PGTUg99_027007</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
            <strain evidence="9 12">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="3"/>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LU65"/>
    <dbReference type="OrthoDB" id="1670005at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000071">
        <property type="entry name" value="Dihydroorotase, homodimeric type, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00219">
        <property type="entry name" value="PyrC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004721">
        <property type="entry name" value="DHOdimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00856">
        <property type="entry name" value="pyrC_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137:SF1">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001237">
        <property type="entry name" value="DHOdimr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="8">
        <location>
            <begin position="86"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1068497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1104432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="395" mass="43529" checksum="D27D0DF831C85727" modified="2019-11-13" version="1">MSSTIDSIDIHCPFDAHLHLRQPGQLLELVVTQITTGGIQSAYVMPNTVPPITSLEMAVSYRQTLESLEPSVQFFLSLYLSPSLTPEDIRQAKSSGVTGVKSYPRGVTTNSTEGVESYEIYYPVFEAMEEENLVLNLHGEVPSSETGDTCVWNAESKFLPKLLEIHRRFPKLRIILEHATTRQAIEAVRACGPTVACTITLHHLSLTIDDWAGNGLNYCKPVAKSPDDREALRQVIREGHPRFFLGSDSAPHPLDRKLPKTCTECCAAGIYTSAYLLPTLASIFESAVPPPLAGQGSSGIEGRTIHSPIPLERLPDFASTFGRQFYQLPIPVNPRTVTLIRDPSASILQSFEHHYPHTSSDNDSSSISSALSQSQKKSVSLVPFWSGRFVGWKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LUJ6</accession>
    <name>A0A5B0LUJ6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_008432</name>
        <name evidence="4" type="ORF">PGTUg99_022692</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000377">
        <property type="protein sequence ID" value="KAA1092220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LUJ6"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF116">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1067533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1092220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="594" mass="65238" checksum="07DBB851EF2C32FE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LY05</accession>
    <name>A0A5B0LY05_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_005405</name>
        <name evidence="2" type="ORF">PGTUg99_017371</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1068909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000441">
        <property type="protein sequence ID" value="KAA1081559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LY05"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1068909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="64" mass="7474" checksum="7C58377E5DC415BD" modified="2019-11-13" version="1">MQNKDPRKCAKCSDLYGVSFRIQLISIQARNFPEMTTVESGASDRFRVSQNCVLWWFSQSLEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1P5</accession>
    <name>A0A5B0M1P5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_014442</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1P5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="290"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="325"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1070511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="47617" checksum="ACD068356AB08C68" modified="2019-11-13" version="1">MTVSRHNTNPSLNTIATSDISFATGEENFPSGLPIADQMTSPSNPDPPTHPISRAIADFKELMSGEDPSLVDFAVTRCFPSFFPSTSLPQDANNLVLTSPTTTPIAVGSAADMPLGFLGSEDTNVPPFMPSLDFGSNTFNCPVPSNSIPPQTFQNGSSDTTLNGPLLSGAIFDETLYSFPSYQTELASVPVAGPSSWPYIAPPMGLNFNFNQSVTNNSILDGWQPDGTLTDRFLGSELSNAPVAGPSSQPYTPPTMGVSYNFGDSVPNSIPYGQQVDQLGTESASEEIQNISKQKGKKRVYESPKGYQSDRKFNVDRRKKPPLNPEDRLRCTKDGHNTSFGTEAAWKRHNRIYHGAPQARPRARRQKASDKKYPCKYANQGCKKTYKSLNDWGYYNHLMKDHHEVVILTGPVRREMVSTSSEEHTPAPGPSREPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1T5</accession>
    <name>A0A5B0M1T5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_024193</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1T5"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022690698" description="GH18 domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="2">
        <location>
            <begin position="34"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="442" mass="48183" checksum="5946DCA8D8F0CB7E" modified="2019-11-13" version="1">MRSSSQVFLATGLLSALFLGSSTHAYSANNRGSPQVKAYYPSYNFEAQPVSKIDWTAYTDLLYFMVIPQPDFTLSYDPKLTRAQGEKLVTEFVAEARKNNVNALFSAGGWTGSRYASSLGQSVTWNPDSNQPLQNHQLRHFSNLTSTETSRKKFAQVLVNFGQKHGFAGIEMDWECDDGIGCNSRNPADVVNFGLLVKEIRALWPHASITAALGISGFNGADGKTATAQETALLAQNLDYVNLMAYDVYGAWAPTTGPLAPLHATCAPPAFGQSVQTGLQVALKQGFKASQVILGIPGYAKRLELVSSKLEEKVVNGKPTYYYQNHTTVTPPGGKFDDKPGKDICGNAQNWGGSFLVNELISNGWLTPDQKHGANGYQRYYDSCSGQPFLTNGKYFITYDDQFSTVDKAKFAKQNGLGGIYFFDTMGPTAATVRAARQALSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M2N5</accession>
    <name>A0A5B0M2N5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004290</name>
        <name evidence="2" type="ORF">PGTUg99_036848</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1090180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M2N5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1070218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1090180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="52" mass="5693" checksum="33BF04682FF59764" modified="2019-11-13" version="1">MVYSSIPASEILTTPGWRKYQAGSISWATCRAELNKASPEEARSVSEGEAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M6C4</accession>
    <name>A0A5B0M6C4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Polyadenylation factor subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PFS2_1</name>
        <name evidence="9" type="ORF">PGT21_012145</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for 3'-end cleavage and polyadenylation of pre-mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M6C4"/>
    <dbReference type="OrthoDB" id="16717at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000237">
        <property type="entry name" value="Flowering time control protein FY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001802">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="203"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="244"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="286"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="328"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="370"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="414"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="553"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="577"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="632"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="665"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="692"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="702"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1071578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="711" mass="78393" checksum="BE5B1AECBD27F123" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8A1</accession>
    <name>A0A5B0M8A1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_010539</name>
        <name evidence="2" type="ORF">PGTUg99_013842</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000236">
        <property type="protein sequence ID" value="KAA1121985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M8A1"/>
    <dbReference type="OrthoDB" id="10268216at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1121985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="190" mass="21012" checksum="C1EDC315600CE1DD" modified="2019-11-13" version="1">MHPSITMCKMYLAYVALGCALLANMVFAGALICDKCGKRSLYLTTGPISTEISCQELRVKIPPCEANAIVERYICKSCGMAAWVPCTACNNRDKDKQHSGLPSYYMPFKCDKCGTGRGLHWTGVTSSYGCGTLQKHSAIQNPNFCRANVAVRQFKCEGESCPWAALVPMGPCNNNDLRKQHTPEIYYYMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9K2</accession>
    <name>A0A5B0M9K2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_000395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000159">
        <property type="protein sequence ID" value="KAA1072919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9K2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1072919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="247" mass="27330" checksum="700E5B12DBB324D7" modified="2019-11-13" version="1">MHNRYRLCQTERLTAHLNSTIWASHLDFGRRATTTSSGSAQHYDSDHQAVPDQTNFGQRLATNSSSVENPTPYVKVPINSFPFNFLRSSRENLTSSPLLITSPSPSTFVVKLNSHLVSDHPRANFPATVLRPTPHLLKFNPDLVCQGASPVLPILLQVVARQPHLVTFPDNLTTTASVDHPSIQVPNSDHRFSLDSVSLPHQYVSTSQLSFLTSPPHALTVGFCFRTTTGFPDLFHHSDSTPVPQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MAB1</accession>
    <name>A0A5B0MAB1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MAB1"/>
    <dbReference type="OrthoDB" id="2495796at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="556" mass="62817" checksum="A13E407790567785" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MGP7</accession>
    <name>A0A5B0MGP7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_033647</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MGP7"/>
    <dbReference type="OrthoDB" id="623670at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF25">
        <property type="entry name" value="RLPA-LIKE PROTEIN DOUBLE-PSI BETA-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022719709" evidence="2">
        <location>
            <begin position="21"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1075323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="11079" checksum="C0FDECB7238BB3CF" modified="2019-11-13" version="1">MISMIFNFFVVLLSVTSSLGAPLSPNRNHLERRFSGQATWFNPALGACGETNSESDMIVAMNQAQKTVSIKNEATGKTVSAKVTDEAIGSLDQGVLPISWQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MM93</accession>
    <name>A0A5B0MM93_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_007419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MM93"/>
    <dbReference type="OrthoDB" id="192832at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF24">
        <property type="entry name" value="GLYCOSIDASE C21B10.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26113">
        <property type="entry name" value="GH16_XgeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="54235" checksum="D89F40C892749261" modified="2019-11-13" version="1">MLSLLINPRSGALASTVLRLQAITALLGFLTSVFADHTGNNAFMQLHRRKLKQDNPLGAPGQPQTSIWSHSQTISGFTFFDSWAFINRWDNTTHSAAYYVGKEQAESEMLAYVDENARAIIAVDTKKDISNDTMPAVFLNTTTGVFKYNQTALRNSIRLESLERYDPGTLVIADFHHTPYGCASWPAFWMHGENWPQNGEIDIFEGWNDNTRGRATLHTTPGCSHDPTGIQTGKVLQETCDSSVNYNAGCSVEDPTTDFFGPTLNRNGGAVFAAMYTNSEISVWRWRRQDVPQDIEDNVPRPETWPTPTATWRSGASCNIDQKFGPQNIIINIAMCGDSDLNTYAQGKCPGKCYDHLLKGSNYKEVYFAINSIKIFKGLPDAVSKVTPLPPPGSKPVVPPPGLKPVIPPTGTKPVVPPLDTKPVVPPPGTKPVIPPLGIKPVVPPSGNKPADPSRHAKSGDPPSNLKLMTSAQDESLLNINSESSDPNPKANVPPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MMH8</accession>
    <name>A0A5B0MMH8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_015850</name>
        <name evidence="3" type="ORF">PGTUg99_007701</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000451">
        <property type="protein sequence ID" value="KAA1077234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MMH8"/>
    <dbReference type="OrthoDB" id="17560at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630">
        <property type="entry name" value="DIENELACTONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630:SF44">
        <property type="entry name" value="PROTEIN AIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033473601" description="Dienelactone hydrolase domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Dienelactone hydrolase" evidence="2">
        <location>
            <begin position="69"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1077234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1092842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="282" mass="31087" checksum="12D432DC0864DA55" modified="2019-11-13" version="1">MMNKLLLFLIVLFAVSIIGDEVDDHRKCLSGFIHEGKTSGKLLSMNGVKVYAANPTSHGRAGKQSRSKGQKAILVFPDIFGIDLINVQLITDKLATDLDTPAYLVDTFSGDDVPDGPQPNQLPVGFNITEWGKKHGPEQVLPLIENVINNLTAQGVKRFAAIGYCFGGKYIFLSSDRNWIQVGSTSHPSLLQVPDDFLELRAKSKAPLLINSGEMDSQFGAEAQKQSDEILGDGKYKPGYKRTYYPGASHGFGIRANLSNPAEKRAFDDSYKQIVKWFDTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MN93</accession>
    <name>A0A5B0MN93_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MN93"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="72" mass="7956" checksum="759972BA661B3690" modified="2019-11-13" version="1">MFSSSVYNPPTPRQTTHFASSHPSVRSTAEPISHCPASSRWRSELSGLPSSRDDQLAPWHPRIDSAPPTKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MNT6</accession>
    <name>A0A5B0MNT6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MNT6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022816551" evidence="1">
        <location>
            <begin position="24"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="116" mass="12667" checksum="A3468B9FEB877733" modified="2019-11-13" version="1">MKLGFFILLSTKILLLVSSGVMGAGPKKPSVDRSQLSPGNVVFKCKLADTYNVPYCIRIPTAKGYFPLDQRDEWGWQHPLFTCITNHVALCCKGITGFSIALADCDGVGVTKLPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRF6</accession>
    <name>A0A5B0MRF6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dihydroorotate dehydrogenase (quinone), mitochondrial</fullName>
            <ecNumber evidence="5">1.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dihydroorotate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">URA9_2</name>
        <name evidence="15" type="ORF">PGT21_016913</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(S)-dihydroorotate + a quinone = orotate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRF6"/>
    <dbReference type="OrthoDB" id="14784at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106430">
        <property type="term" value="F:dihydroorotate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF4">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="14">
        <location>
            <begin position="163"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="618"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="626"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1079597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="667" mass="73088" checksum="9159BF7695CC218E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MRK5</accession>
    <name>A0A5B0MRK5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000137">
        <property type="protein sequence ID" value="KAA1078714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MRK5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="441"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1078714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="456" mass="51619" checksum="810FE3D15873B3D7" modified="2019-11-13" version="1">MDVTSLLNNGSATNSNEEPNLEENHLEERQSERSRLSERTTIRTTPDPETDLLDYSEAMTQDTSLVPQQRSDFASLSSSLPPINATGTRIPAEGAPPSDFGVSPALINSRATSLALETMSNRSTPVPRPVERLNFAVPTNTATGLQIQSPTPRQLNQTLIPAMDPSQAPDGQEAIIVDNEQLETIINLFNDQWLMFVQAREHHNVPMMRFALQQAVGSQEVIRNLAGGEEMLRICENWIAREELADLERAERDNPTPQVQRLAITQRAHSNTISPSPAPLPLTRQTSEITYLGRGQQPVQDQPPTQRPQTAQSHHSVRGNHLPPPPPPSTQPPPASAYYNQHRQVQHHPYYRQDGSQHQQQYPQQFVPNYDAHQQAQVAQTREPQRRQFHPHQQGNWRGYGRNWRTPRDPTTRLLEVGDYLMRAERIAGRVFRVRGRGRGRNQAQRQGNQHQEPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MSL3</accession>
    <name>A0A5B0MSL3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SGS1_49</name>
        <name evidence="8" type="synonym">SGS1_74</name>
        <name evidence="8" type="ORF">PGT21_022966</name>
        <name evidence="9" type="ORF">PGTUg99_010551</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000446">
        <property type="protein sequence ID" value="KAA1078859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MSL3"/>
    <dbReference type="OrthoDB" id="2503239at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="51"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1076884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1078859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="294" mass="33472" checksum="AB5970B1EE4E0C44" modified="2019-11-13" version="1">MPPTKDKSTKVRLSKQWRRKEQDDLHQAIIDDCLPRYPSNEPPKEVQVNAVKSLVGGKHTFVMAGTGCGKSRISEMYYHLFAKSKKAVVLVLNPLDALGDNQVKEKISQKYTAINLKKLTFDQDVADEVLNGKYNFVYLSPEVFLNNQMFTDVYHNPKFQDRLALIVVDEAHMIYSWGLVANKQAKKSSAHKRHQDRSVFRPSYGDMGRQLMATEDTPILFLSATCRPKAVTEILKSLKIPEETVNFVKAELTRPEIRILRFPMESSLKSANNLLSMFGRKQDVKSSKLVPTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MTE4</accession>
    <name>A0A5B0MTE4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MTE4"/>
    <dbReference type="OrthoDB" id="2507457at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="594" mass="65284" checksum="26686DEAA58EE3EB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MUM2</accession>
    <name>A0A5B0MUM2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_018950</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MUM2"/>
    <dbReference type="OrthoDB" id="10306342at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1080741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="385" mass="44174" checksum="8F9217CEE594EF5E" modified="2019-11-13" version="1">MRHKHQRPHKSSQQTGQSWSTNQHQSAKTPRKQAVKYVVYPYKQDQRLLEDSQGEHYTVERLDHEETSAVDYPSNSAKDHLSLEPPSDTMFEDKEDDCNSKNSKNEKHKLDQIDLDQIEWDNRSDLDINAAPYQVQPEQSSTFKAFLEDGVVHFFPESNSTQQSEPDFDLKNYRAEDVDNWASTLLADEFEHLCVMGTNARTKSFQQYAISNLNQLTQTLTQQSSSTHEHLEHLVSTSNGCQDYRNYHHHQPDDISSNNFDQQRLASPDDESRSAFYNHSGNCNSHLEAFDDIGFDDSTFDNGGFDNDDFEDGGFNNGGFDNGGFDDCGFDDGCWNFDSSLLHLFHFSILQYLFLTIIPLSTNNYIVCLVASHEFKHQILVVKEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MV83</accession>
    <name>A0A5B0MV83_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein PBN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_024194</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for proper folding and/or the stability of a subset of proteins in the endoplasmic reticulum. Component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase GPI14.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PIGX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MV83"/>
    <dbReference type="OrthoDB" id="5546453at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013233">
        <property type="entry name" value="PIG-X/PBN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040039">
        <property type="entry name" value="PIGX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08320">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00780">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1079789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="247" mass="27294" checksum="18B8D2947F968FD5" modified="2019-11-13" version="1">MVGLTTELSERVYGFHPSLRTTVTLSSDEVEGLKECAVGLVFAGLPGTVIVDRYQLRELYEEGRLSEPRVVPKKDGLLDLLVWTDPDLEGPASRPDPPGLGGLMVPLPLPFTGSLSIIVPLHLRYLPPILDPKTDRRPVREVLIQPPSLVKWCNQSTPVASETSLPAITSDEIRLLNEFFSSPFQPLQGLPTLDAHLHPSSPIRLSVPVGVQHDEWPVLIISTAAVWLAFAWICSCLYYKKHLPSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MW45</accession>
    <name>A0A5B0MW45_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_028103</name>
        <name evidence="3" type="ORF">PGTUg99_023167</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MW45"/>
    <dbReference type="OrthoDB" id="2507345at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1131195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="163" mass="18009" checksum="DB71B13B93323F77" modified="2019-11-13" version="1">MQVEVLDNTDSSSSFNKSEDNSNSKPLWRAPETTGDVTFQSLHRGSLDRSSSVTASLKESTSKGKLSMSTNTISGAQAAQMISSETSNPLSIQPRPPLSASSSNLVKRIVENQNLLIWQAYDDWRQKFPRLQFDDKDEEIEENEAESEAETEPFTSDSAADES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXC1</accession>
    <name>A0A5B0MXC1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_025800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXC1"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="73"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1080936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="212" mass="23335" checksum="8B4A005E38DDC13B" modified="2019-11-13" version="1">MPLTILPTTSPLLSPSSRKIPHTKIPPNPTRLSDNQHACIRSWFVFLMLFFSKSGETHKNQAQTDGFINPTILLLTETKEAFALFDKKGTGTISRESLGDLLRALGQNPTQAEVAELAASAPRDIDFATFSAILNRPGGFSPAGTAEDFIRGFRVFDKDGNGYIAAGELRYVLTSLGEKLSDEEVDELMKGVQIESDGRIDYEKFVRGILQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYL0</accession>
    <name>A0A5B0MYL0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_028760</name>
        <name evidence="2" type="ORF">PGTUg99_026026</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYL0"/>
    <dbReference type="OrthoDB" id="10445114at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1081068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1131231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="110" mass="12354" checksum="853E7A05AE9DAE41" modified="2019-11-13" version="1">MLSLIDLLQKLVERHIDCVCGGFSCCNTYLIVCSSRDLVLDRIYQILGYANRSWIYRNTNRPAMGAQAETVDPISPKFNISPSLLWVPNETVKCHIDGPIGGSLKQQQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N0W5</accession>
    <name>A0A5B0N0W5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">STE20_3</name>
        <name evidence="9" type="ORF">PGT21_019150</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N0W5"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="8">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="174"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1082885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="312" mass="33741" checksum="6FEC8386AC0E64CB" modified="2019-11-13" version="1">MHGRGLRRGKEDLWDREFPGGVCKHRADPEVDRESRQTSDNQALDVGRLNNTNTTNNNPISPISPDGIDSKDSSKPKKDRRETTIKSVFGGFVSSVNDLLSNQKKVEVSAPYNPVHLTHVGFNSNTGEFTGLPKEWQIRLQESGTTKVNGELQFDDVWQKFGHVAEADLHQPLKDPSGVVFQNPRSAPAPPPPSRPNLALCSQTTLPPTGLTSASSMSRRPSDSNGPVTSPVQLTSSKSFTGRPSKSKARPNSLDAPSIHAQPFPAKLNNTVAKDIPIPQRPAPPKPLSSSKLGKSSSVKVSASSSTKKFLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N305</accession>
    <name>A0A5B0N305_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_017157</name>
        <name evidence="4" type="ORF">PGTUg99_005993</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="4 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000438">
        <property type="protein sequence ID" value="KAA1083212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N305"/>
    <dbReference type="OrthoDB" id="2505307at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137370" evidence="3">
        <location>
            <begin position="24"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="367"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1083212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1094296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="389" mass="38991" checksum="568A8F401EFA6014" modified="2019-11-13" version="1">MRQLKSAHVVFSLFLLTSSVCSSENNLCQCGEPQFEGRNVDNNVVDPHKRIMTAPEQPNTNVQPVAQEIPTAAGDELPPGGVSSGGYQPSSRGSPTLRSTSSTNELPPGANTSESTPLPVPIPKQSGGPPSASTGSPNSQTQPDPGVSGAASSVPDPTLSPSASSNKNGTASVAPGASAPGRQTGTEARVNGSATSTPGSSSALFPGSSSATSPGSSPGSSSASSPGPTPGSSAGSSPGSTSNLTSNPTPSNPSSLPPKQTSTLPASAEKSQGSPGKPTDDQKAAIIVSSVLLGVLLIIGVISAFLRRKRNKIEALRRQEEGTMQAKPGEFGRRGTVLVGRGLESSHGLPSSGGWLDNRSSEFGTTARTLSISSSSTSADSNEAELNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N458</accession>
    <name>A0A5B0N458_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014631</name>
        <name evidence="3" type="ORF">PGTUg99_003649</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N458"/>
    <dbReference type="OrthoDB" id="2500854at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="455" mass="52249" checksum="721B5442EBC33666" modified="2019-11-13" version="1">MSDNDDSSNETADLVVKALKNIAIRYQSVDLHAASRQPPWDVDAETWVHDNWKFARQEPLSIDEISTLNDLLCQMQTRHLPSLQQQLVNLSRSLDLANLPNNPHPKLEDTLEILSQLGDSLDQINTFVHSIAPIVVNPLQQTGKIDHHFGGLKNYRSRELIEKFNNLERESLKWLFDSLRRFIRASPKDPWTILNDESILGSLGKDVIEAIDQSYHAISEIIKWSKQSDFSILQDECRRDANNLEPILEGLNRRIALTTRLEEQLRLIDEGEFDTDSSSASGDEDDEESKTSLSTDSSEQIFLRPSLMKLAKTTIPLIKLIRIFFNKLSNTPTSKAPFTFGTKLSSAEIREIDCEIGGLSCSIRNLLNLIFYMFDCDEYVNDMKNLEQHFKELPRDFNSSIVLLCCYLLPVNDQLDLPVSGNLFKTWFLTLREQFDLAYDNFRQAFSRFKEEIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4Q5</accession>
    <name>A0A5B0N4Q5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TEL2-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_021540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="49511at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="2">
        <location>
            <begin position="276"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="3">
        <location>
            <begin position="1071"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="608"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="982"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1043"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1062"/>
            <end position="1073"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1084225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1295" mass="144703" checksum="A4BE76D3ED2FD0EE" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N5I1</accession>
    <name>A0A5B0N5I1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_005886</name>
        <name evidence="8" type="ORF">PGTUg99_011986</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000214">
        <property type="protein sequence ID" value="KAA1122903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N5I1"/>
    <dbReference type="OrthoDB" id="2508326at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="383"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="420"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1083753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1122903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="600" mass="68006" checksum="B493F9894BA8F6C4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7X1</accession>
    <name>A0A5B0N7X1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_032721</name>
        <name evidence="3" type="ORF">PGT21_032781</name>
        <name evidence="4" type="ORF">PGT21_036975</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7X1"/>
    <dbReference type="OrthoDB" id="2506685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1120012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="278" mass="31648" checksum="0B1E6F245BFD46B3" modified="2019-11-13" version="1">MSLPYTVPDLEDGFAPTTPLGNRPFASGSNSNWNTGFTALPPPMKKDKGNMPVNQYREPQFEEEMSDSEDPTPQRQVKQTEPPVTGYGLHNARGLDGEPTERRYGPRIIKEPGLVYDGTNFTRFLARYERAALAFQASDYEKALQIGRFVHKEELKLELKAMEGYDSFNWTEFRKAMKESWGELDKTILYTTADLVKIVEEYSRNGGLKDYKEYKAYLGKFTTILKYLVDNEHLNKKQDASLLFTSAFSKESQKNIKRTLVSNGQLPKGPYSSSKPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N7Z6</accession>
    <name>A0A5B0N7Z6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mitochondrial ribosomal small subunit component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RSM19_1</name>
        <name evidence="5" type="ORF">PGT21_006801</name>
        <name evidence="6" type="ORF">PGTUg99_007291</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000252">
        <property type="protein sequence ID" value="KAA1118391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N7Z6"/>
    <dbReference type="OrthoDB" id="2043at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF8">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1085427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1118391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="116" mass="12830" checksum="24D7F9E6A3393D77" modified="2019-11-13" version="1">MRLAAHEDPCFVPALRDCRLRGSKTGATEPPYHSGVSQGPYFTAFPNLAEALQTNTAIRTSARACTILPNFVGLKLLVHNGKEYIPVNITPQMVGHKLGEFSPTRKRFSYKASKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDF8</accession>
    <name>A0A5B0NDF8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_008236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDF8"/>
    <dbReference type="OrthoDB" id="2500162at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="530" mass="58538" checksum="7AE8FD2178AF67CB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NDJ4</accession>
    <name>A0A5B0NDJ4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_020638</name>
        <name evidence="6" type="ORF">PGTUg99_008658</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000106">
        <property type="protein sequence ID" value="KAA1085819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000282">
        <property type="protein sequence ID" value="KAA1112141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NDJ4"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF37">
        <property type="entry name" value="LD47843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="747"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="4">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="579"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="675"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="293"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="10"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="278"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="398"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="490"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="585"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="602"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="640"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="680"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="694"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="713"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1085819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1112141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="770" mass="85143" checksum="29C797B3A07F1E68" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NE73</accession>
    <name>A0A5B0NE73_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SsDNA endodeoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RAD1_1</name>
        <name evidence="7" type="ORF">PGT21_017264</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NE73"/>
    <dbReference type="OrthoDB" id="337581at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003021">
        <property type="entry name" value="Rad1_Rec1_Rad17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02144">
        <property type="entry name" value="Rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01245">
        <property type="entry name" value="RAD1REC1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1086952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="115" mass="13223" checksum="7622D12C11C290A5" modified="2019-11-13" version="1">MTYEADELAQMEFPVERQMVHLIMKSDWLKSAFSQFDNKSGTDITFMFTPTPSTGWSAPREPTTYHNNQESSRRSLRVDDTGFLSLQLLIPLEEASTMKRKCGYVEFMCVPIEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NF74</accession>
    <name>A0A5B0NF74_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Vacuolar protein-sorting-associated protein 36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ESCRT-II complex subunit VPS36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_031501</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the endosomal sorting complex required for transport II (ESCRT-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the VPS36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NF74"/>
    <dbReference type="OrthoDB" id="271448at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000416">
        <property type="entry name" value="Vacuolar protein-sorting-associated protein 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037855">
        <property type="entry name" value="Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128:SF12">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1087433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="219" mass="25114" checksum="5ABB2C192C0EE9BE" modified="2019-11-13" version="1">MISNDQAYAKELAKELAGLLTRTMAGQSAIMFHGFHDLAVGIRPLDEIWCIWNRARGISLVSPADMISACQHLPEYTVPEIRLRTFKSGLRVLHTPYFSVHEFEHRLLRRLRNTKEGGAEEEDGHVDTTSDLIDPLDGTKLMKMSTLEIAQSERLNLNLTNELIESIEQTPSHPSEYHHHHPLSFDKCSLVRDFDPQTGLTFWFENQISDYHWEESNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NGQ3</accession>
    <name>A0A5B0NGQ3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphotransferase</fullName>
            <ecNumber evidence="11">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HXK1_9</name>
        <name evidence="15" type="ORF">PGT21_036515</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the hexokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="11"/>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NGQ3"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24087">
        <property type="entry name" value="ASKHA_NBD_HK1-2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000156">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000009">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 11 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="13">
        <location>
            <begin position="71"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="14">
        <location>
            <begin position="275"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1087776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="526" mass="57994" checksum="3195F5FB9149362E" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHG1</accession>
    <name>A0A5B0NHG1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2507336at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="410"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="725"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="765"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1105"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1233"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1504"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1679"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1754"/>
            <end position="1775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1868"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQ88</accession>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
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                <person name="Nguyen-Phuc H."/>
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                <person name="Miller M.E."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    <sequence length="131" mass="14306" checksum="DC22F940436D4BD9" modified="2019-11-13" version="1">MMCFIWMIVLMALGLLCGMSSASGWHKVARHSRGCKSTDILWQVNNSNGVVTLICTSCGENSTPIPLTPCLVCKKRTVPSPAYTDRDCHKCQHKVEHGYHVLACNGCPHTLCNYQIPKATCPNCGTPPLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSX1</accession>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Sperschneider J."/>
                <person name="Matny O."/>
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                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
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    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1081349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1092013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="74" mass="7889" checksum="7437BE0F1E2CE8ED" modified="2019-11-13" version="1">MVNAKYIIMSISLLCLGTKVTSSIVDIHGLASAADHLPTTALAEADPPCKHSPVKKCEACHQCVKCKPRHCTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUE9</accession>
    <name>A0A5B0NUE9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_007829</name>
        <name evidence="5" type="ORF">PGTUg99_019743</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUE9"/>
    <dbReference type="OrthoDB" id="406505at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22271">
        <property type="entry name" value="DPBB_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF21">
        <property type="entry name" value="EXPANSIN-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137567" description="Expansin-like EG45 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="38"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="119"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="166"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1092583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1093682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="41804" checksum="503C26366675B7A3" modified="2019-11-13" version="1">MFIRSRLAFLHLLVLVVSQILSASPPYDVGKMQPNIGAGSAGTQGMSAPGATGQSGASGTSPDSQGAGGAASGGSPAPGGAPSSGSGDSAGAGGGASAAGASGQPDASGASSGSGGAAATGDSSSAAGAASSSGDSAAAGGMSAPGASGQGDASGASSKPGGTAGTAGDASSAAGASSTSGDSAGGASPAASTGGNSSGTAADASSTSADSAASGGSTGSGGSTGGPYDGRTYQGRATTYDAKWSSGNCLLSRWPQPKGLGPVAIAESLWDSARMCGACLSITSSFGTHLGIVTDQSDVKGNSIDMGPDVWSQVSNHQKSSALPITWHLVPCNFTTPIEFYNKNGASQYYTAIQVAGSNKPIKSLEASSAGYGSASGGGGGGSSGGWVKLVRASNSNHFGPPSGKGGLGTKADLRITCDNGKQFITKGVSLVDSTKPTPASGNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NUN5</accession>
    <name>A0A5B0NUN5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011166</name>
        <name evidence="3" type="ORF">PGTUg99_029280</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000373">
        <property type="protein sequence ID" value="KAA1093782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NUN5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="142" mass="15513" checksum="7442002B44004D7C" modified="2019-11-13" version="1">MEAYGRQLVSVTTTAKSYVLRNQGIQNNTPSTYPINSSSSPPLTNSSRDSGGLPVGSAGPADIDLDPKTRGSADTHTFHQTSNRSIILSNTNYTNRLTIHHQVRHRSGSINHQLPNRLLKQPKKPPRGTIFTGLNLLCHTCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NWV4</accession>
    <name>A0A5B0NWV4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_021257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NWV4"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1093014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="69" mass="7493" checksum="9A2B9AEB102B8324" modified="2019-11-13" version="1">MAFEEFSLLPPRIHTLTSHFSDRGSCLGDNRLQVKTLRLQREGDRVVAPAAGLRPAAFNEIKAIASAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P069</accession>
    <name>A0A5B0P069_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018939</name>
        <name evidence="3" type="ORF">PGTUg99_027233</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000375">
        <property type="protein sequence ID" value="KAA1093379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P069"/>
    <dbReference type="OrthoDB" id="2506225at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1093379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="222" mass="25228" checksum="364ED6A402951EC5" modified="2019-11-13" version="1">MTKLITISVNGRPSRITQDILKRTAMRRKREAEKALVRARDSLKVLLKAKYPTRRPVEVGDTNSDTALLSKGYVYETPSNRRRGGGHGAPFLRSPLAYCPVAAVEPEVPQAPWVIFESPSFGILDNLDMTTTSDFATNVLNLYNRAQNMTDAQLTEAIASDEWWSEVERCWNQTFKPQEPEVTPLPEFETLPEFETMPEFEALPEFEMTAEEFPLLFELLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2D6</accession>
    <name>A0A5B0P2D6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000790</name>
        <name evidence="3" type="ORF">PGTUg99_002616</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000069">
        <property type="protein sequence ID" value="KAA1095571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000041">
        <property type="protein sequence ID" value="KAA1135079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2D6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1095571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="244" mass="27006" checksum="DA9EF973DC3C1006" modified="2019-11-13" version="1">MEPTVQWTKVTSSSSLRAQPWVVNLQSMNWDEFQHGALLFLSSSSAYLLPAMEYANLGNTITWFGSISNDAKYGSPRGFRIQGELDYLAFGAAVHATAPSQVLVKLMMKDPRNDDPELKYEWRLTKLNREGVPIKPTELLTQSMKSQDSSVGLNHDADDLPSSGEGSNGRQPLDGPIPKHQRYNPRTPSLSEDSDLEVVALPQRLFLPDLVAGLVSPNGQLNSKAPRTPMAPPQKEMDEVNMDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P2E8</accession>
    <name>A0A5B0P2E8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dynamin-GTPase protein, variant 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DNM1_1</name>
        <name evidence="7" type="ORF">PGT21_027453</name>
        <name evidence="8" type="ORF">PGTUg99_009116</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1128947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P2E8"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000029">
        <property type="entry name" value="Dynamin GTPase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000383">
        <property type="entry name" value="Dynamin-like gtpase dnm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF235">
        <property type="entry name" value="DYNAMIN-RELATED PROTEIN DNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="6">
        <location>
            <begin position="23"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="5">
        <location>
            <begin position="728"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="505"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="648"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="536"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="552"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1094640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1128947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="847" mass="94343" checksum="35E0280510770CB3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P341</accession>
    <name>A0A5B0P341_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_033138</name>
        <name evidence="3" type="ORF">PGTUg99_000285</name>
        <name evidence="4" type="ORF">PGTUg99_003114</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000371">
        <property type="protein sequence ID" value="KAA1095583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000157">
        <property type="protein sequence ID" value="KAA1127637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P341"/>
    <dbReference type="OrthoDB" id="2507837at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1087527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1095583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1127637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="552" mass="60111" checksum="08440BCA8CBD07DB" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P3A1</accession>
    <name>A0A5B0P3A1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002706</name>
        <name evidence="4" type="ORF">PGTUg99_010453</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000011">
        <property type="protein sequence ID" value="KAA1137174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P3A1"/>
    <dbReference type="OrthoDB" id="10261522at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000019">
        <property type="entry name" value="Thiamin pyrophosphokinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="260"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="440" mass="50528" checksum="E948672BF0FF7462" modified="2019-11-13" version="1">MSFLPVLKTCHNHPTLNPLLQQQTNNNNNNQQQQLIPFYLHQPDQQQLIKPIRLPPPPPPPKRLTNRSLSSSSTTTSNPAELTKFRPLASYLTRSNSSDQLDNHPFNEQVYQPPWDQAISDRDSRQPIGFLRPAIIKALIQDNQHMNRIHSQACWKLFYPIEADDDQDDLGDRLPVKVSFEDWINQAEDRIETRQEHLDRLIRGWKENGLFLDQLSGWRNEEYSVYGPKDDDPEESDHEPTQLPGSNLAFRIERAAVGLFGFLSFGVHLTAYVKKDGEYFFWIPRRSATKATWPSKLDNTVAGGITSGETGFETIIRESFEEASLEEELVRTHIRATGLISYTHRSPEGWVQPEIQYTYDLELPDEKTIVPKPNDGESEDFKLMSFDEVSEALKRAEFKPNCAAVLVDFFVRHGLLSEHNEPHYFAVSTLVKQPVFLPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P737</accession>
    <name>A0A5B0P737_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_015516</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P737"/>
    <dbReference type="OrthoDB" id="10338788at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023062991" evidence="2">
        <location>
            <begin position="19"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1096400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="272" mass="31442" checksum="C6DD2920ADFEF6E8" modified="2019-11-13" version="1">MVVLSKGLLLCLVWHVTGPNLFALSTLVHEAEESIGRPPVVLNDQNAYRLTLLDENELDQHYPSEYGSIPQEQAERKAEIESMKNEHEKIKKRIKLLKALPGQPSKAKVLLKELHKNLDIPMKERFSRLKLTPTQSPETSFNLVEDTSELENTWNALQTQLIRTVYSKKHVEPDEADFVLEFTKSFFLLGDYIYKYGLLPAPFIKQIEIFHGPTIFDIISRHINYMFRGNQGNFFDHAESVIPQLEFLKTAPSVKHFHRTIKGQAFHVIIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PA82</accession>
    <name>A0A5B0PA82_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PA82"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1097973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="68" mass="7853" checksum="1B38D8DA42619CF6" modified="2019-11-13" version="1">MACKLNGKVTPPACLVSWRSLTTFAIWQDFSPSRKEVPDYRYNASRFGELMLKVTKVTIFIRARQTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PF11</accession>
    <name>A0A5B0PF11_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030569</name>
        <name evidence="3" type="ORF">PGTUg99_022981</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000342">
        <property type="protein sequence ID" value="KAA1099220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PF11"/>
    <dbReference type="OrthoDB" id="2505311at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1084549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1099220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="180" mass="20363" checksum="BDF19247C9B6E1EE" modified="2019-11-13" version="1">MEFPLVGVFEILKIPNLEQTQREDLDFLCAFIHDCKQFVSPAISEGLSRDEILSAIGWSKDLTQLEILPRYRDEESIRKNQEAYSKLMEASKKAGGILWDIFSTLGNLAAENTRAKMKKLASDGLVSSLAFPSNGFHTDYQVENEEESDPPLSFAMMIPTLINIHFLTIQSRVMKSHHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PHF1</accession>
    <name>A0A5B0PHF1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Tryptophan synthase</fullName>
            <ecNumber evidence="5 13">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TRP5_1</name>
        <name evidence="16" type="ORF">PGT21_005613</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the TrpB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the TrpA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="5 13"/>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PHF1"/>
    <dbReference type="OMA" id="VDTARHS"/>
    <dbReference type="OrthoDB" id="10050244at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000151">
        <property type="entry name" value="Tryptophan synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000001">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000004">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF3">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="15">
        <location>
            <begin position="376"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1101057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="724" mass="77372" checksum="B5026F5498FBB0BD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PIA7</accession>
    <name>A0A5B0PIA7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Fe(2+) transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MRS3</name>
        <name evidence="11" type="synonym">4_1</name>
        <name evidence="13" type="synonym">4_2</name>
        <name evidence="12" type="synonym">4_3</name>
        <name evidence="11" type="ORF">PGT21_035502</name>
        <name evidence="13" type="ORF">PGT21_035584</name>
        <name evidence="12" type="ORF">PGTUg99_011341</name>
    </gene>
    <organism evidence="12 15">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14 15" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 15">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1102059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PIA7"/>
    <dbReference type="OMA" id="WRPMRGM"/>
    <dbReference type="OrthoDB" id="43906at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048250">
        <property type="term" value="P:iron import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000127">
        <property type="entry name" value="MC family mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000172">
        <property type="entry name" value="MC family mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758:SF4">
        <property type="entry name" value="MITOFERRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758">
        <property type="entry name" value="MITOFERRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="13"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="118"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="209"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1098461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1100300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1102059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="306" mass="33066" checksum="984E508E766A9D6C" modified="2019-11-13" version="1">MAEEVDYEGLQNASLGVNMMAGALAGISEHVVMYPVDSIKTRMQVVAGPAMNNVNSGVTTEVYKTMTSTFRSVATTEGTKRLWKGVSSVFMGAGPAHAVYFGTYEMTKEAFGGNQRGQQILATGAAGSMATIASDALMNPFDVIKQRMQIQGSKHKTAISAARAVYQAEGLRAFYISYPTTLTMSIPFTAVQFSTYEELKRLANPVDAYSPITHVVCGGISGAFGAAVTTPLDVCKTLLQTKGTSTDPEIRNCRGMLDACKLIHRNMGLIGFTRGIVPRVLTFMPSNALCWLSYEFFKMFFLGETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PJF3</accession>
    <name>A0A5B0PJF3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_029907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PJF3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="88" mass="9712" checksum="5267B2D58DB21A40" modified="2019-11-13" version="1">MPTRSNPDPLLPLTDPELILKNGRRQARLEKSLKQAKSTTDLVDLPPLPPSPTMSDSPETSTNTKATNPLSLDEYLKGLVETNRETND</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLG0</accession>
    <name>A0A5B0PLG0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_019187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLG0"/>
    <dbReference type="OrthoDB" id="2500322at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="477" mass="53523" checksum="61B4228498A5A0BF" modified="2019-11-13" version="1">MADPVESVTVEEGTEDKDRLDGDQKVEETKDNDAMEEHSESGSEASSSSGATTPSGPHGVVLKALRKLLRNYHFSLNQICCSSQEDLTLDDLQDKVEIFKTLQTSFLPSLKQQVGALLDSLDLSESAINPCPNPESVAKILSELSATLHDTRSAVEELALEPPLQAASHDRHLKFLKRYRTSPLLWKTKAVIQDDVCEIFQAIEGLLINLEHHQNPKPGESSQSEFHGSDIIDYLFNPVKCKQRINTTWAKIRIVIDGIIEWSNLSDFAVLQRDWRSSTRKCNRMLEALGEIVNGTYWRKDGNRMSAEAASDPKKVEAHKARAIQLAKTGIPIVKLGRIFFDKLSKTCLHPLPFTMDTEMSSDELESLVKKLESFYASIDYMSGHLCVIYESDKDISRKINSLRNVSEGVISELEEALLALSFCLIPASQTPTTDRRPASEQHFKTWFLEFKNQFILACTNLIDATDTCFGDQLEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLJ7</accession>
    <name>A0A5B0PLJ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLJ7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1101460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="12734" checksum="24BD369B905C8EC2" modified="2019-11-13" version="1">MTISILLPPPSTPSHSPWYSSNYNLINTFYRPHQAKMVNSPPHPQQEKESGESHIAHILQRLRLNNHHHDHSNQQTNPTASDHPQPPPEQHVPSPNRISYQRPPAQPQTAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PQC3</accession>
    <name>A0A5B0PQC3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_004302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1103907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PQC3"/>
    <dbReference type="OrthoDB" id="10284575at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="491" mass="55386" checksum="4BC2CE0B66345F17" modified="2019-11-13" version="1">MRSSNQSKKLARLERSFALTGSISSPYSTISPPVHNSQNSEHTTQHDKSMRFSRITKDKGFGARHRDILACAIGVAFACNSALGELGFDVDDLFHDWTYEPPNLDDIWRQPNSPAPEVQLTSPWNAQHTPTWTPQHIDSEPPRAAGSEARKRDFELGPSTFNPSAASLPLMPHNENRVGLGANQSDQRPAGSLLNFPDILQNFHFSRLDGPFTDSVSVNPTWNPSTHGTLSQVHINQEPTIPTCQYSQAAAADAQNHELDLWHLPWSPAPEISPQLPASKRPKTQRNPSPINIATENSLEKKHREEHHNLEAPTDQNANQLPATLNGADAVIKKPVRTKSRRTRINALPETPQINLDPTFPASTATALWSSSTNTSQEKMSQLTGPDALKFERLVFKEETFKYTGLWPDHYMHRDKIKRLNRAVRYFPEYDGLFMDKDRPKENASLIEQFTHHKWNELTHGLSNTGKRSARKPQRFSHLIPILCRHDSYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTH2</accession>
    <name>A0A5B0PTH2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_028084</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2504331at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
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    </evidence>
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            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAA1104611.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="2145" mass="238162" checksum="0106132E5FD5AE20" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PTZ6</accession>
    <name>A0A5B0PTZ6_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">PGTUg99_011777</name>
    </gene>
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        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
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                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    <sequence length="410" mass="46901" checksum="F29019F6239F2B57" modified="2019-11-13" version="1">MTSGVLERACVLVLLFMGALGVLGMESDVTCVAESSIPREKALPMNLNQESQVLEEKIGVKNKPNGSKDSSFACETQHIYVPQETNGPNNQLPIQKDLKASKIQKILERCAEISKEFIHSKEDYSYFQKLFPRNYRQFSLHIKELKDLANYLYKRRIFEILIGRPLILEGGGVRLSNQLVANIDIILIKDCLSLFEEFVETGVYQVYKDSKIVNLMISDWTNLIVHFIHDLVNLEIVPQGTILEFLRKDHGKLLWSLITIKYCPFDVASKYLNFDLRLSIAEDRNINNTKLRDIFQAFNEDAWKQAEFKLLKAQLLDKTVGVHSEFSHLITEYFLRIASPDKKTSASHVEHLLEGLMDVGISPLFFSSPKNPSIPSLKIIMNKQILHVIYPCLCEFLRDILSCCLVLKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0PYA3_PUCGR</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
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        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="28"/>
        </location>
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            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1105911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1121014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="204" mass="21910" checksum="588C34D679DBB44A" modified="2019-11-13" version="1">MTLSGTPVLRLLGFITLLLQSRNNTVSGKVFRCDQSLAPVTQYQPKSTTVNPLLSSVASGTRPSIMATPLSNPVQPFSINGPPPTKELACKSESGEKTFAFCVADSCIGTATCEGCRSDFGMMVKVKCMSYHIQNQQNFCEDINGIKFVCAGSCEDRTGKPAIFVFLSSFISSTPKTFNPCLDKVDHATTTDTPPGEADVFKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q0G9</accession>
    <name>A0A5B0Q0G9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Yeast cell wall synthesis Kre9/Knh1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_036801</name>
        <name evidence="6" type="ORF">PGTUg99_001700</name>
        <name evidence="7" type="ORF">PGTUg99_002144</name>
    </gene>
    <organism evidence="5 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000172">
        <property type="protein sequence ID" value="KAA1126154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000080">
        <property type="protein sequence ID" value="KAA1132416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q0G9"/>
    <dbReference type="OrthoDB" id="5316007at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052479">
        <property type="entry name" value="GPI-anchor_Adhesion_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35185">
        <property type="entry name" value="SERINE/THREONINE-RICH PROTEIN ADG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35185:SF3">
        <property type="entry name" value="SERINE_THREONINE-RICH PROTEIN ADG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="4">
        <location>
            <begin position="38"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1106648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1126154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1132416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="248" mass="27859" checksum="8BF344A2ED777BAB" modified="2019-11-13" version="1">MVQDSFLLRLTSSLTLVVVILIGVLTPFRSADAYLITSPAANDFWYFGSKKMLRWSSVSTDPVRFSVAITNHDPSTYPSALSTLVVKGIRKKWGKYELPASALKGLKEGRGYQINIMSLEGGAILAQSPIFTISRVDDESGEDEEDDDEDEVIDYADQQEEAPTTGTDQPHDAHKANKGKDDKDRYKRLGKLHQKIHRHKKAKSYRSRKEFQLAKSKSNALYKSIPKFDHSRSACSRSPRNPKIAKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q5J5</accession>
    <name>A0A5B0Q5J5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q5J5"/>
    <dbReference type="OrthoDB" id="10330915at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10449" checksum="ED5652F912706924" modified="2019-11-13" version="1">MQGYNHEEAQRNHRELVDGQANILRRLDNIDARLDNIEGTVSSLLRVHQNASELLRPHARFPNLNLRHRNGAVGQGNGGVGGHRGGRGGRGGRTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q696</accession>
    <name>A0A5B0Q696_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_022113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q696"/>
    <dbReference type="OrthoDB" id="2505605at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1108698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="187" mass="21319" checksum="F2D9D240369748BC" modified="2019-11-13" version="1">MASQMQSNIKLLVLKQGISTSDELGSFQTSFNPLQQEQPFSTGPWLIDPTPEEELFLRRNKLESVTFLLRIKPLTFDSNVENLRVVCCHVATKFPSPRILARHLAESIVVHDMNVRFRCSEKLMKTHVTPIDHQTHGELFKVVIRLSQSECIVNWTIFRQVVLPTMISSTGMRSCSFKSSPQCCLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6E9</accession>
    <name>A0A5B0Q6E9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6E9"/>
    <dbReference type="OrthoDB" id="10271750at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023030583" evidence="1">
        <location>
            <begin position="24"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="191" mass="22334" checksum="92EA99C28908C96A" modified="2019-11-13" version="1">MHPRRRIVEILVLCCLLTARHMAQVAPCQLKHARVGEDMTGSTSSQTRLSNRESLDLLPQISDIPAERDEEKTRRQFGTSVQALQNRQEATMKEDFKNLHRQLQIFTEAAKGVYKEKNIPDLLDSVVKLVRIYNRIPTFKHDQVLKWEDKILVLLPQKPNDLYRIDEPPTEIRPQDDIYQGKEYVILIQFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q9P7</accession>
    <name>A0A5B0Q9P7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7719 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_001624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q9P7"/>
    <dbReference type="OrthoDB" id="5597489at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056136">
        <property type="entry name" value="DUF7719"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37846:SF1">
        <property type="entry name" value="DEACETYLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37846">
        <property type="entry name" value="YALI0B21296P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24841">
        <property type="entry name" value="DUF7719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7719" evidence="2">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1109881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="80" mass="9196" checksum="C5683301555B30B9" modified="2019-11-13" version="1">MIAARREIRRADGADELSTIGGSYMVYIMNRLSYLLVMRRVPPLGALWIYAVVRMHLSHAVLSLILVALWAWIMNLSFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QAL0</accession>
    <name>A0A5B0QAL0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Pep3/Vps18/deep orange domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGT21_005921</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">21-0</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1109993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QAL0"/>
    <dbReference type="OrthoDB" id="1845386at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:endosome organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16462">
        <property type="entry name" value="RING-H2_Pep3p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007810">
        <property type="entry name" value="Pep3/Vps18_beta-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058919">
        <property type="entry name" value="Pep3/Vps18_RING_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF26">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05131">
        <property type="entry name" value="Pep3_Vps18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26148">
        <property type="entry name" value="VPS18_RING_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1076"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="domain" description="Pep3/Vps18 beta-propeller" evidence="11">
        <location>
            <begin position="46"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="737"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="Pep3/Vps18 RING C-terminal" evidence="12">
        <location>
            <begin position="973"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1110"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="932"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1110"/>
            <end position="1130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF26148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1109993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1218" mass="136759" checksum="BF1C5DE6AADBCBDC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QB82</accession>
    <name>A0A5B0QB82_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_025361</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000027">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="STKc_Cdc7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000176">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001504">
        <property type="entry name" value="Related to MAPKK kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053235">
        <property type="entry name" value="Ser_Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361">
        <property type="entry name" value="MITOGEN-ACTIVATED KINASE KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361:SF433">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="42"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="304"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="513"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="636"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1310"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="656"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="730"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1310"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1360"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1387"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1467"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1110556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1479" mass="161598" checksum="8A7364AFD91C38A5" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDC1</accession>
    <name>A0A5B0QDC1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1111119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QDC1"/>
    <dbReference type="OrthoDB" id="2246127at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="97" mass="10551" checksum="6E4A128E7AAEF797" modified="2019-11-13" version="1">MLNYQTTQSWTACEDLATNGEEDHEFLVAEESNTEDNTFSDNELDSCASDLDTSSDLSDLNSNDEADGEDFELSAEDLDLMRELGGVGDGDLDSTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFD4</accession>
    <name>A0A5B0QFD4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/pantothenate metabolism flavoprotein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012983</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFD4"/>
    <dbReference type="OrthoDB" id="70224at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="2">
        <location>
            <begin position="226"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1111819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="365" mass="40976" checksum="9006E2F459CBB1E0" modified="2019-11-13" version="1">MEQGFSSERYFQTQSPPSHLEEIVSRVRSFVTFHASHHPNRRIVLITSGGTTVPLEHNVVRFLDNFSAGTRGSASAEAFLRTNQYAVIFLHRSHSLEPFSRHYPHSTNPFLDLLAIQEPSSDSASENQSPPSQQPLFPILSPHLVNHHQHPAVSTENPAHLDHHTISIPAQHRPAMLSILQAHHLVHQLNLLHTISFVTVNEYLYILRAVAKVLGEPSLGLGRRAMLYLAAAVSDFFIPQQKLSEHKIQSGKGSLVIEMDAVPKVLKDVTNEWSNQAFIVSFKLETDSNLLIPKAKAALTRYGHHLVIANELHTRKTSVLMIDREGTIEPIKNDDPLGHEIEELMVLRLVHRHLDWIQASSTPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QFQ1</accession>
    <name>A0A5B0QFQ1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000016">
        <property type="protein sequence ID" value="KAA1111814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QFQ1"/>
    <dbReference type="OrthoDB" id="2507343at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="193" mass="20235" checksum="80264CF4CEE2A566" modified="2019-11-13" version="1">MDPRMLLEMYMSMAGARNTAQAAPSEGNQSRRRGRRSSNSRRRAPAPAPAPAPAAAQPAQPVVPNPLLGDGRPPGIKLPFVDSEAKAKAHQDLRSKALCLPTAHPAGARHPRVYTLIGRGNLGGSPQRGTPPRPAESSPEVDPQTDGLPAQIIPAPAPVSQVGPSRRERTQGRTDPYAGSRANRARGPAAPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QI06</accession>
    <name>A0A5B0QI06_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004669</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QI06"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="73" mass="8691" checksum="F50F44CB79899742" modified="2019-11-13" version="1">MSNLEQLLLPVLEQQQQLLISERYRQSNDQAMRHTLESFFAQDDANQRRDNEEALFLFWALAHLGTPGQYRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIL0</accession>
    <name>A0A5B0QIL0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIL0"/>
    <dbReference type="OrthoDB" id="2503229at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="377" mass="42336" checksum="DFB690035BE09C3D" modified="2019-11-13" version="1">MTAAQKSIQDPTVTNGTENSGYATDQSQTRCRRSSRASSVVVAPNMVAPSPDSRIKLTRPASTTQKRPAVAEPESSSESESEADTQRESGSEDKPGETETAAPPRKSKPQKKKPRRKTTSSAAKKNKPAQASSKKKKKSQKETAANKPVPLPAYDYDQESNDESIEIRPRAAAAKAKKDEGFAKIEEFFYPPTWKEGDPKDVYLNFKCRWCKVVYRGNQNTNGNLVCHRDGFTQAGKNNRGCVNREKAKQAGMKLPLSVAERRRLENLSGNGQQSGISQFLQVQPLFANRVLNQLVMIWQIRQALPWSRIEDPYLRAAFQYTNQKAVLYGQRWSADESKRLYSMLKPQVFSELNVSFIPESYSESKLKFELNYPPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMS2</accession>
    <name>A0A5B0QMS2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QMS2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1114576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="109" mass="11928" checksum="4CD7B8F3A1D0A07F" modified="2019-11-13" version="1">MSHFKEIPRIGIELGSHTPSTTDPRDIKARSVPEIKLKAPSGKSLEYGGLGLVVATEEGLPTVNYPENRYLSAAYLSLAPEGIVNKPKTWAPLSPPFQLPKPNQTHRQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QNQ0</accession>
    <name>A0A5B0QNQ0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7918 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_012487</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1114524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QNQ0"/>
    <dbReference type="OrthoDB" id="3237202at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057678">
        <property type="entry name" value="DUF7918"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223:SF5">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25534">
        <property type="entry name" value="DUF7918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="37"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="333" mass="37204" checksum="FC00F47A80E6633A" modified="2019-11-13" version="1">MPINAATGTSCTINLLPPPPTTTSSSSSTDQIPAQIPCKEYKHETTTDPTTGAIQETVTIESQQACPFEILIDIKPTAYSTLTANSSSRSSKIKPQDYVIHRILDGISAGYQKLSKTKSHRLVHMTKTSSRDKLSFRRFQFAPITLVDPDDYETPNQGSRNPTDTICEDEEVIKSLGTIQVDVIRADLVHRLRKPAKNRAAPAPSTNQMKFSERSKKARLLNTAGLSDTFPSNGPPRPMKWHIKKQDPKPFLQFVFKYKPRAILEDEGTIARDPSQADQINQNIEDDDQDAHETDRKPSLKRIKSEATIANDHQIRQKKKQKIIDLTGADDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QP91</accession>
    <name>A0A5B0QP91_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_030349</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QP91"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="81" mass="9109" checksum="AEAA1B16B39E5F8B" modified="2019-11-13" version="1">MYGKQKKNRRISCILLEVSSKLSRSLKIATIDQLLTLHLHTPLKTTGAQTPQVKIRSFVRTDGKLVGLKSRLITSKKGHQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QT07</accession>
    <name>A0A5B0QT07_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QT07"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1116336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="232" mass="25882" checksum="6142613BD08084D1" modified="2019-11-13" version="1">MSTTSQSENESRSPARTAPSTQVNRSNVANQTSHRTRPYPNALPRDSGGRRPFSNNSRQNRSLETPTDPGSPTTQPLPTVGTVRHFGRPDTGVVRTWPPSILPFETSEADINDRYFENLIQTFDIQAPYMDFAEELAQYSVLLYSVMLVRQAVESLIRSRDAAQPGSSAPVAIPLSVQARIYPYQNHFKNHAKEVLMSPTLDVYSCSPVRGAPPAGRTLLDQVLESFRHWQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QUI9</accession>
    <name>A0A5B0QUI9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prokaryotic-type class I peptide chain release factors domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_027967</name>
        <name evidence="8" type="ORF">PGTUg99_032125</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1116958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QUI9"/>
    <dbReference type="OrthoDB" id="277888at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000065">
        <property type="entry name" value="Peptidyl-tRNA hydrolase domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052405">
        <property type="entry name" value="Mito_Transl_Release_Factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46203:SF1">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION RELEASE FACTOR IN RESCUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46203">
        <property type="entry name" value="PROBABLE PEPTIDE CHAIN RELEASE FACTOR C12ORF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="6">
        <location>
            <begin position="38"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="141"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1098076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1116958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="222" mass="24920" checksum="49963BC1DA11CDAE" modified="2019-11-13" version="1">MRAILNNIFFSIGRLRTFSQSAIQLKKKKRYIPPIPPLDENELIEQFVRGSGPGGQAVNKTNNAVSLIHKPTGIRVQAHTHRSREANRNQARRVLAERVDYRLNRGNSKIEKMWERERKRKLDKVKKQIKKQKELEEKLLKATTTTTTAATPTSTTAPIPTPPTPTPPTPSIQNKQTSKPKTKTKTKTKTKTKAKTRAKTTTTPTTTTTTALPVNRSTPPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVI1</accession>
    <name>A0A5B0QVI1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVI1"/>
    <dbReference type="OrthoDB" id="71672at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050315">
        <property type="entry name" value="FAD-oxidoreductase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400:SF1">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="498" mass="55424" checksum="2C485755CC6F6F9E" modified="2019-11-13" version="1">MMAIQQIRNFLHSCQAPLVAFRNRRLRPSWNPGFNQADGFIGQANSIDSFSQRKSVACSSKTSEEVTSVRQTIERMKMERQNASEHTREDDNKSNAPSRMSRKFSWIRSNGTKIEPGTDPEVSQKVKTIKEANKGTDDHKESAKNNSSEVKAVTSGASRGSSNAPLSSGSESVEDFEKREGPSARSSSQRVVDKNHYTKLLREPEMKQTDADTESKRASKWGFTLKSLSLLKSNSSTRTPEDTKRALRKSWWIKSEVAPNTFLPPSPNALSLKFSGSVSPLATDKPVEKKVAVPESLVEGLTSPYKNIYQPEILQFQLELLEMPKDSVPWPWGDPVPFWKGTPVHKLSWGGFEVDLVGIRQGTTKNSYVIRVRRPSRLDEYVLRKESQFKNYCRRLSKDFPNAHVRAVPPPEISLEDDLFSLDGLMQENEQHLISTKTSGGFGEGAAPQKSHGRGRMAQPPSLTDRGPSLTGNPFFVDPFTALPLDPVPVPTPGGNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QVS7</accession>
    <name>A0A5B0QVS7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_004364</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QVS7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1117348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="105" mass="11477" checksum="6AA2782138BA8144" modified="2019-11-13" version="1">MATAQGGSAKYTAGTVDQALEQIRLNRRTHQSRANSSFMITRLGSTFPVEEAQAPFVVRPETKNRLPIYFGSSVGRANDFKHLHQALMLGLGSTSAQHQTSSEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZF2</accession>
    <name>A0A5B0QZF2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZF2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1118677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="101" mass="11189" checksum="75A0BA61E0D2CC5C" modified="2019-11-13" version="1">MDDIELNAVQLEVTCPKVCANLTRRVDLELRAVELEVESSCDDSATLCAFSPARLRLKFVAWTLRDSTSPSGLSSITLWNYLVGSDRVWKGKCAVNKAALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QZX7</accession>
    <name>A0A5B0QZX7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7918 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007753</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QZX7"/>
    <dbReference type="OrthoDB" id="3237202at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057678">
        <property type="entry name" value="DUF7918"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223:SF5">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25534">
        <property type="entry name" value="DUF7918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="49"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1118841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="374" mass="41716" checksum="C74DC516BEFB1354" modified="2019-11-13" version="1">MPTNAISAASCTIKLLDPTKSTASTACPEYLHQSLIDPKTGAHQEIVTIESKASHRFEISYDIHPTASDLLAKKSSNHSRSNKTCRSLRMHDYALEIWMDGLEIATSCSPRSRKLGPMSMSEVVENETSVRAFEFAPLKLVDPDDQLGDDEDEDDRGEKLATKLQVCQDEKIIKALGKIEVRFFKCKLTTREAKKSRAKSTGIDPTWTKTTNEMRFSEAVKIVGLSATAGLSQPTIIDPSPKGGKFYRIKSQDENPFLHFIFHYKPRPVLIAEGTIVPPALRRNGRDPSTAIRIESDSEPEKEQSKRSKKEKARERVVKKEEEGCRCACKKREPDDDAVGDPQSSSDPKRSRPSDGEEDQKPNIDEKPNIDGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0E5</accession>
    <name>A0A5B0R0E5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1118970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0E5"/>
    <dbReference type="OrthoDB" id="2496592at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022924223" evidence="1">
        <location>
            <begin position="28"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1118970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="140" mass="15432" checksum="E325036BBAF20683" modified="2019-11-13" version="1">MTPPNFRYILLATIFVVFLNLTNQVAGATLERRLAKGELQPLCDPDGTLVTPNDCDVAVLTLPFTNKRSILSTQTTVVSVFRSCKVYVFCDQKAQTTSMALMSNNLQNGGYTRLLNECGNHKKSGVIFFDTKCSIRTTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0N2</accession>
    <name>A0A5B0R0N2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0N2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="181" mass="20085" checksum="482D18A280BF2CB6" modified="2019-11-13" version="1">MTSSTTSGEPRPIKAPRETPPPDSKSHSRIKALSPASQPHRSRLRQDQLDSALHLELHLGRSSTWADTIFFGGSHGWPLRRVGPAADGPQFCVDSTPASMWAPDGHKVRSTWSLESPITSALRRLAKDKLEDPHQRILDEAEVNVHRRGIDVSNKPTGSTSARMWDCGTNIEKKVGSWNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R0V7</accession>
    <name>A0A5B0R0V7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R0V7"/>
    <dbReference type="OrthoDB" id="2506728at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1119197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="375" mass="42209" checksum="20CF9A611557B464" modified="2019-11-13" version="1">MFTDSLSSANRQLEAVCSQVEQQNNSKDNQGLLLAKLEKDVHYLTIQANGLEPRLVAEKNEYLQRMSAIQKEFDTLNSSLGPISLMAPLLQAFLESQLRSSSRSKEQFEKSFACHHNSHNGQGEISEKTVIRHSSAINMGRWREGNSTETSSKRPERHKTKRPDSSGVLGNVSEPRLLDDAKKNEVKQPMYIGPEDTQMSRNQETEEVSSKVEAEHEPTTTQASESHSKDEVQTRADEPIELPRGRAPSSHLAARRSMSRRKSQAVRPNSTQQDPGSIDHALDLQRAHSKKRARSRTPTGLDREHTVHTKLISSTQLRRISSQQTTHNPASCTILSEDCHHQQSNQVIGQAQINSVVHKKPLKKNRRPLLLEEHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R3M8</accession>
    <name>A0A5B0R3M8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_037203</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1120146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R3M8"/>
    <dbReference type="OrthoDB" id="10561744at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022858043" evidence="3">
        <location>
            <begin position="20"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1120146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="243" mass="27490" checksum="26D599DF9C94A587" modified="2019-11-13" version="1">MQFLQILPVICVIATPKIAAGPARTPHVKQTDLNSAAVISNRNSFEPLGQFIDDSDHNQSYEGNLSFEISQDSSSNKEEKWGHILKKSREGAPGEQPIKSNRHEKKTEASSSTDSSSLTGISEAELDPETWVYYRAVTTALGALALDYIAQSIVLKNPTLSDYLLNEPLKSYQHIRIDHWFLLASFAKFKALFNPFSYMQLKIPMKEKLRLNLMEFLSLTFVFMHFVFLVPPIPLILKSTFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RG88</accession>
    <name>A0A5B0RG88_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013540</name>
        <name evidence="3" type="ORF">PGTUg99_034154</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RG88"/>
    <dbReference type="OrthoDB" id="2502393at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="288" mass="32200" checksum="8D1871BF018A6690" modified="2019-11-13" version="1">MPGIELPSLGTQDGQEPALSEEELSHLASSLALEASLDPGRDGETRRPAPSQANPALSREEQQQQSSDDLIPSGDPCHHNLKLRNVSVDLLQRITARTVLQFILSLLDGEDLAHFVRSPSATLPTTLAYSPTRHSVISSVSKNRDSFARQARSETQLIIAQSLLMTRAQILEEFESDMTDRNPEEDNHQDEAETPKEENEAEGRRTTLEERLNEQNVSRIVEDELKDLIRLKFTDYSHPFIDHQAAFERSISLVQASELISFSAQVLSWNRLFDLLFSSNSGSNPPCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RIJ5</accession>
    <name>A0A5B0RIJ5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013711</name>
        <name evidence="3" type="ORF">PGTUg99_034016</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RIJ5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138135" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13588" checksum="0A74BE81EEF3F0D7" modified="2019-11-13" version="1">MNIRIIVFMLALGFTHTACAALAPAKMTCSHCEGSNVFQTTLPGYPTHGHCYYYVWDLKELCLEERTKEYYKCHSCQQYTLQNQSNPGDKCTHTNKQKIVWNPPPQAHDPLKAELHSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RPB8</accession>
    <name>A0A5B0RPB8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_031178</name>
        <name evidence="5" type="ORF">PGTUg99_032454</name>
        <name evidence="6" type="ORF">PGTUg99_032684</name>
    </gene>
    <organism evidence="5 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000171">
        <property type="protein sequence ID" value="KAA1126673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RPB8"/>
    <dbReference type="OMA" id="KVWQYIR"/>
    <dbReference type="OrthoDB" id="10251073at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10567">
        <property type="entry name" value="SWIB-MDM2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="196"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1081200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1126673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1131362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="287" mass="31208" checksum="8BA67571A03D3B9D" modified="2019-11-13" version="1">MSLPPNIANHVRSYLTGPEVDLQTVSAKQIRKKLATIFPDLDIKALRSEIDEISIPIFHEIKESVEGDVKPAPHETSVNPSNPPQQVIKQDHKPDIKPTTSLPPLALPPRGTPGVVKTGDSQEAKPTIPKPAPQPPASTTPRSAKSTSTKSKKRKSAAFVDTDDEEGSSSTASKKSGSAKKPRPPREGEGPGSNRGIHVELNCSPALSNVIGVPVCSRPQVVKKLWEYIKANSLQNPQDKRQIMCDEALKKVFNQNSVHMFTMNKLLASHLFKPDEVVQPPPPPPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RRE2</accession>
    <name>A0A5B0RRE2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)</fullName>
            <ecNumber evidence="3">4.3.3.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SNZ1_2</name>
        <name evidence="11" type="synonym">SNZ1_1</name>
        <name evidence="11" type="ORF">PGT21_000810</name>
        <name evidence="12" type="ORF">PGT21_001507</name>
        <name evidence="14" type="ORF">PGTUg99_013348</name>
        <name evidence="13" type="ORF">PGTUg99_013927</name>
    </gene>
    <organism evidence="13 16">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15 16" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="13 16">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="3"/>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000147">
        <property type="protein sequence ID" value="KAA1127932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000046">
        <property type="protein sequence ID" value="KAA1134744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RRE2"/>
    <dbReference type="OMA" id="RYANRGW"/>
    <dbReference type="OrthoDB" id="1660966at2759"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000001">
        <property type="entry name" value="Pyridoxine biosynthesis protein PDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00343">
        <property type="entry name" value="pyridoxal 5'-phosphate synthase lyase subunit PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="10">
        <location>
            <begin position="76"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1107058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1116118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAA1127932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAA1134744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="363" mass="38039" checksum="03E764A4B806CFF7" modified="2019-11-13" version="1">MSQINQKLDAPVSSSTSSISPNSSPSNPPPTSINPLILGANSAPATNGSQIGSTRSATAMQSSNSIAGTSLGTGTFGVKTGLAQMLKGGVIMDVVNAEQARIAEEAGACAVMALERVPSDIRRDGGVARMSDPAMIKEIIDAVTIPVMAKARIGHFVEARILQAIGVDYIDESEVLTPADPEHHINKHAFKVPFVCGAKNLGEALRRISEGAAMIRTKGEAGTGNVIEAVRHERAVLADIRRASVMSDEELYTFAKELSAPYHLLKEVARTKRLPVVNFAAGGIATPADAALMMQLGCDGVFVGSGIFKSDNPALLAKAIVQATTHHNNPQMLAEISTGLGPMMKGEGNLQSKDVVKMSSRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RUX3</accession>
    <name>A0A5B0RUX3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_017336</name>
        <name evidence="3" type="ORF">PGTUg99_031903</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1086961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000137">
        <property type="protein sequence ID" value="KAA1129397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RUX3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1086961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1129397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="69" mass="7383" checksum="D1A92BAFAD1A617E" modified="2019-11-13" version="1">MKTTPRPLSLHATACKVVTLSGMDEHWQSTQSGKSWSHTEPASAGSSAAALRPRGSIKSLDNRFSTQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RYA3</accession>
    <name>A0A5B0RYA3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_032742</name>
        <name evidence="4" type="ORF">PGTUg99_023725</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000106">
        <property type="protein sequence ID" value="KAA1130820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RYA3"/>
    <dbReference type="OrthoDB" id="2497442at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474900" evidence="2">
        <location>
            <begin position="24"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1113505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1130820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="384" mass="44683" checksum="805A8DEC385E212B" modified="2019-11-13" version="1">MMTLSKKFLWYIALRMLADSRLAQSIPTEKLDTGLREVRRAGNCPSDSIDHILWPGEKELRSQGFGWKEANSQPHSKRKAEIDRMESRTKQISEKIRAFKSKYLATESDESHPTSSELPDGSRIERTIFNLESVHDYLSSKCRRKFYKSSQGSPHTALPLDNFNEEEDYPLAEIHDGSPLPEGERVVAKVLYGPNRSSIFNNLWGDLMEQLPSQKVSNQHQSEFESDLLEIMSLLGNYMTVYQLKSTDFFRKNNIVRSLKPKELSKVIYLLALSKGYIMEDFRSYLESRKAGKQNFIQNIATSFNDLDPEFLMTSKSLEPYRKEIEALEKKDKDLLVYNCRKGLLDRLIRQQEQLRRLVQQRTEFVVRRPTREVKRNDLRSQGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RYU9</accession>
    <name>A0A5B0RYU9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_002083</name>
        <name evidence="2" type="ORF">PGTUg99_008641</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1093893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000115">
        <property type="protein sequence ID" value="KAA1129993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RYU9"/>
    <dbReference type="OrthoDB" id="2502444at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22744">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1093893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1129993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="44295" checksum="1667BCA95B678D0F" modified="2019-11-13" version="1">MIARQPININHGSPQLQTSLSQPMLRKMLLLCFTSLIMAEVTLVAGMEGIKAGAKFSTSDLKPEHTASYDIKAGKAQEKIMGPTSKGNEKVGYAYPYYAPASPPIPVYQPERKSSLGRLASKLKNYYFSFKMEFERFCSFKWLKDLFRTNHHNNYPPPVFYPIYHLQPPFRPHYHHAQFPEPAWGEPLHSNIHHHAGQENEVFSPSDSSVQSTDRKIKEVVEKLRGTKVTAEIPSKNYWTPVEHLPEHYRTTDYIKEKNGLFRALAHFAYEDQEQYWRVRDELIAYIAKHPGQFEKYLTKKGEHDSIEVDDHLYRLSNGESIDGAELAAFSELYKLNLVVVTKNGDSVAAHQHVVDPSNKEFSGVILQDGHYQLLTHSKNPTIKIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S1G3</accession>
    <name>A0A5B0S1G3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030687</name>
        <name evidence="4" type="ORF">PGTUg99_031647</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S1G3"/>
    <dbReference type="OrthoDB" id="2503926at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="245"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="312" mass="34436" checksum="D0C6FB1E57B438DE" modified="2019-11-13" version="1">MPHPIVFAAGMAIIVGAGYVIYTELNRKSDEEEAYQTYCATIRAEKESRNRRLQGLRDAELDDKAQQSSSSLKNIHHTSLRHRSTNQSTPQEKMDYPLIDLGQQFYPPIPRSHRSTSPSRSSSSSNSDLNKISLFNPILDIPIPDPVIFIAPSMEESVEAEGSASRELQDSTEALLSQDQDQSTDVSDETPLIDWKPQEESTTLGRLLGLEPQDSDPLQPASMQASEQPEYSEEPQRSQTDREAAQSPSQPSAPSSPKSPFELPVPQLPVDTSFASDSDDWSVADEPVQTSTNSPSLDGNWSDLGSDSFERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S855</accession>
    <name>A0A5B0S855_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_014037</name>
        <name evidence="3" type="ORF">PGTUg99_009414</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000069">
        <property type="protein sequence ID" value="KAA1133992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S855"/>
    <dbReference type="OrthoDB" id="10557939at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="93" mass="10715" checksum="8784C405EA135673" modified="2019-11-13" version="1">MLIDHTWLVLRGLKNNWIQLVEHQFDVLPKSSVIKRNTTKTAPKAPVADQEEESEEETDDSDEDDFDDSDDDDSDDDDNDDDDDDDDDDNNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S918</accession>
    <name>A0A5B0S918_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphomutase PMU1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_005982</name>
        <name evidence="3" type="ORF">PGTUg99_025743</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S918"/>
    <dbReference type="OrthoDB" id="496981at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1133583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="43488" checksum="EF269C6ECB11CE99" modified="2019-11-13" version="1">MNLNSLPPHPDQKQPHQEKPHERPAKSNWFDRKTLWIGIILILYGIFELYSHQSNVPKNGKNPNHSLELLDFSSLPGFFIQDDRDQDPQDFHIYHPNSSFGLIDHRSPDRWIQFQQKIKHLIEHSPSDAKHKVIFIARHGQGFHNLAESKYGTPMWNCYWSEKMTDGQLVWGPDARLSALGKDEAQQAAKAWTRELAHHAPLPELFILSPLSRAIETMLITGVWKHVVNHSEYNEKPQPKIIVAEKWRENIGLHTCDQRRSKQSISNDFPIVEFENGFNDHDLLWTQDLQETDQQLDIRIRAALTNVFLDPMSWNLTYISITAHSGVISSLLRVLGHRPWPTETGGMIPLVVRATPKKTPTRPPNPGPSATKPPCPTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SAB8</accession>
    <name>A0A5B0SAB8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_027148</name>
        <name evidence="3" type="ORF">PGTUg99_017680</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000042">
        <property type="protein sequence ID" value="KAA1135056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SAB8"/>
    <dbReference type="OrthoDB" id="10275550at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138292" description="TNFR-Cys domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1093164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1135056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12243" checksum="E9AB690581DB6E4B" modified="2019-11-13" version="1">MHPWLKTKAAILLTSCIAFTKQLDCPLRRCNGVSNSTGSNYTEPRATCKTMTTCPEHTAQCHPCTQLVPLEYHECTLCGHQWTAPMLNRCPKANTNHANQQCARPNTSAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LNE3</accession>
    <name>A0A5B0LNE3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_014390</name>
        <name evidence="6" type="ORF">PGTUg99_014916</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000196">
        <property type="protein sequence ID" value="KAA1065891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000314">
        <property type="protein sequence ID" value="KAA1104981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LNE3"/>
    <dbReference type="OrthoDB" id="432528at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052124">
        <property type="entry name" value="Rab9_kelch_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647:SF1">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137016" evidence="4">
        <location>
            <begin position="31"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="711"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="836"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="938"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1016"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="713"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="746"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="850"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="879"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="902"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="938"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="951"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="985"/>
            <end position="994"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1065891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1104981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1098" mass="117551" checksum="03F3668085E21306" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LV51</accession>
    <name>A0A5B0LV51_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000184">
        <property type="protein sequence ID" value="KAA1067578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LV51"/>
    <dbReference type="OrthoDB" id="10677938at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="385" mass="43957" checksum="558B5DAED79BA8BC" modified="2019-11-13" version="1">MAQHHLGKFSKRMLINGERGVKITNTLSHNTPTMGPHIADTGLNFHPASVNGKDDRMIYPHESFVLPKPESKQAGLTLMEFHAIAGRKMNLTPRQMDGFLENFIASTLKGKLSGQTFTLLDLVLMNIKWGEENIMPVEHINKLKNWLKLLEDNGTGTLEEYSSITGDKISGILDSETAQRFADWHVVKEWRNKFKDTVKLDLSFLINAHMKWADRNILSFKNPYNEILRSKDKDVVLEELSEKLKHLELEDYIKIMDQTLSKRQRESLEWYLISGSKSSTHDSSGVPWGYDNLARGIKDWKIKEAEKGSPFGPESYVTNPKEPIGLPGGPKSSDAPLEIKIEPLKSSQEETPAQIMKASSWKQQFKLLIKSFWKALRRYFQPKHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LXY1</accession>
    <name>A0A5B0LXY1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020941</name>
        <name evidence="2" type="ORF">PGTUg99_031600</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LXY1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137122" evidence="1">
        <location>
            <begin position="25"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="146" mass="16402" checksum="D89780C602AFAEEA" modified="2019-11-13" version="1">MKASFPCVFLTMLISTFLANRAIGRIAVPVEELQHSGEVTPNLSTQAWVVAPAVSHFEAHCRDQQVVLSIIQGSGLVFFPHMPSTNWLAYNQLTRQVIVHIEFKNEAGQPTGDTHTFQPLSYKKINVPFRTELKVQLISMKLIIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LYV9</accession>
    <name>A0A5B0LYV9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LYV9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1069053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="192" mass="21181" checksum="AD8E88A530572546" modified="2019-11-13" version="1">MGFRIRGASNQPHKIQPQPFPDCRNTNTHSPQGPRHQIHTHNPQGQPHHIHTYGTHGQPHQIQSQASWIPLGWTHPDTTSAVSHRNPQSTLSNVPHPQASLNTAHHQPHSTTPQHSGMTPHQAPNDSHTQLTHSPEHEDPQLDPFTTPNVPPQTAEQLESEILTQITDFSNATQGVTRGQPRGRGRGTSRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LZH4</accession>
    <name>A0A5B0LZH4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">INO80 complex subunit B-like conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_019058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000183">
        <property type="protein sequence ID" value="KAA1069260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LZH4"/>
    <dbReference type="OrthoDB" id="2021186at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029523">
        <property type="entry name" value="INO80B/Ies2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561:SF12">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04795">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01406">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="2">
        <location>
            <begin position="299"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1069260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="431" mass="46881" checksum="BCE30AD427401514" modified="2019-11-13" version="1">MADNSDGPSNAQNLYSEEDETPHTTTNIGASKVLDGPTRASSTSSLSEAQDPDADAEGEPDDLDADADGIEDDDAMDYSYEQQDIDGARIDDGDESAGQEEEDEEEDGEDEDEEEEEGEDDSGEEMDLDENASSPPPSPPSRLSDQAHQNGSESNKKPLKLKLKLATQPAASRPPAKRKYVRKAPLVSKSPSARSRSTNDKKANSSNKRRSTGSDEEDDELAEDSLGYSDGDDDRAGNQPGPYQASNGSEGASTGDDTMPTAGQLTVRQRAKEYGEPVTELQSLPMTDSKKKKELTEADRAMEKAEKSRKRKHQNEKKLEDEKTETINRLLKGQVGKAKGSTKTAGRPIDTTEENTNQVVKAPAVPVLPTMIRRVSSIRSGEYIASLNIPSSILKEHEDHFLLSPSSKVVYPPPLRPPPKTWKLVNGVRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M1Q2</accession>
    <name>A0A5B0M1Q2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">5'-3' exoribonuclease 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RAT1_3</name>
        <name evidence="3" type="ORF">PGT21_023663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M1Q2"/>
    <dbReference type="OrthoDB" id="372487at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xrn1 helical" evidence="2">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1070772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="230" mass="26385" checksum="159ADCB916C435C6" modified="2019-11-13" version="1">MMNDDSPIKDFYPEEFEIDMNGKKMLWQGVALLPFIEQDRLLNSMKDCEDRLAPEDKIRNEPGHVVMFCGEAHPQYENFCKIYQKRDNKEAVTLDPAHANGVFGSALPDPDCIPGSTFYSPLPTVGLPDISNDKSIFARFFVPSQLVPHRSLLLSGVALERPILNNHDRDLTRRGGNQAHGGNQHYGSDGFHQQRRDFGDGPGIMTHYHTNNSKLQWRRWRLLELQPRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3J1</accession>
    <name>A0A5B0M3J1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_015561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3J1"/>
    <dbReference type="OrthoDB" id="10503117at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1070548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="211" mass="23222" checksum="85F6697086327C4B" modified="2019-11-13" version="1">MKRSGMIQPSPDSRRSITQPLLSERTSIGASSQPKKQTNAPSDSDSTPQATPVQSQQTRKKAPKKPKKAGPSAANSESNTTAIDIDLAQDSDEENSKVKKRQRKNPEEDDVKTFFSEPFCRKDDPADKPASTYSCLWCKKEVRVSASSLSNLCVHRDGSRQSGRVSDGCPNRAKAIAAGAKLPPTSLDKEKKKEEEWKWRPNDSFCQSGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M3T5</accession>
    <name>A0A5B0M3T5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_006830</name>
        <name evidence="3" type="ORF">PGTUg99_009013</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000211">
        <property type="protein sequence ID" value="KAA1123109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M3T5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137142" description="CCHC-type domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1123109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8327" checksum="EE0D8ACB630E36B6" modified="2019-11-13" version="1">MRLNIPIFLLAATLLSTSLAMPPLAEHEVVHTEFHLSPHQGHHEGGSGQQLDEYQACSHCGSERHDSDECPEAPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5A7</accession>
    <name>A0A5B0M5A7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5A7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="186" mass="20310" checksum="1C43D6395BCFF46A" modified="2019-11-13" version="1">MSMSKDIATRATRKMKGLKDQSRLGAKEGATQPTTPHLQATATQSAGPTTQATLASSTTKEGVNTANEIEHLSNLSQVGERGTNDTEGEEPIRETSKDNGEDEVIEGQLTKGCRPDRLPETATTYRNILSSSEDLVPMLVLKFTSQNLVDHECYTQFQSISMGFTNFGLLCSHDSNRNSRLEDPKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M5G5</accession>
    <name>A0A5B0M5G5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1071771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M5G5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="298"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1071771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="577" mass="62367" checksum="5725A210C104D3A3" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M776</accession>
    <name>A0A5B0M776_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arginine N-methyltransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RMT2_1</name>
        <name evidence="6" type="ORF">PGT21_026400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000170">
        <property type="protein sequence ID" value="KAA1072033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M776"/>
    <dbReference type="OrthoDB" id="19014at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019702">
        <property type="term" value="F:protein arginine N5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000927">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051038">
        <property type="entry name" value="RMT2/GAMT_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026480">
        <property type="entry name" value="RMT2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379:SF1">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51559">
        <property type="entry name" value="SAM_RMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="RMT2" evidence="5">
        <location>
            <begin position="136"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1072033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="374" mass="42242" checksum="CA5D5DAE0E896178" modified="2019-11-13" version="1">MRALIGEVIADEEGRRGYHSFLAICESGQLSEIKEVLQQQFGSDSLPSLSLQDDDGWTALHYAAKSGNPRAVEYLLQQGALWAMVDNLGYNAGDVAFSMNHVEVYQIISNHGFRAEALRLTMEATYPEEEGATEAGSTATNNARFLASKLTFKTSASGQRLCIDSEGNGVMQGWETEIMRETAKRLCEPFLGRNKTSDFELSILNVGFGLGIIDSFFQQYHPARHVIIEPHPDVLQFISDQGWPDKPGVHIYPGRWQDFLQDVKEGKIQADFDVIYWDTFSEDYRSLKGFFDNVFDLLSGSEARFSWFHGLGATSRTLYDIYTEMAEMDMREAGLRVSWSKVPVDGGEAVWEGIKRRYWDIPSPYRLPICTYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M7B7</accession>
    <name>A0A5B0M7B7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000166">
        <property type="protein sequence ID" value="KAA1072615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M7B7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1072615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="198" mass="21689" checksum="84365F31DA24C4E1" modified="2019-11-13" version="1">MCIPGDKTGQEDPRNGSPTSQASGSSGQCSREDSISIVETHPDEFRSIWPGWGCVRTPALSADEEDVNVADEEETGSRSSALINKMKSLIKETPKKALKKKTAKQVQHRCVWFDSQGLKSSSAGQKTPKDKMPAKRSDKMHIDPPALAKLKANLLQIPAASTAISSRSLLPILLLRPEWKQSTNIRLDSPAGPRNYPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M8Y7</accession>
    <name>A0A5B0M8Y7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGT21_000321</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000162">
        <property type="protein sequence ID" value="KAA1072749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2507344at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022698">
        <property type="entry name" value="OrsD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF145">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12013">
        <property type="entry name" value="OrsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="1004"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="1191"/>
            <end position="1342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1072749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="1632" mass="183138" checksum="3EC22394C456D067" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M979</accession>
    <name>A0A5B0M979_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_002934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M979"/>
    <dbReference type="OrthoDB" id="2507324at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023086285" evidence="2">
        <location>
            <begin position="23"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1073181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="456" mass="47750" checksum="EFC4DB8A40C87618" modified="2019-11-13" version="1">MLSIYVLTIAICLFQLEVGAAATSISGMSRAQVLEQKREIVTIAVSSSEAEGVHDDLSKTRELVLPNATSQKKRVRRDLPAAKGKAKGKNATPTAPLNPPAQFQLRGNDQDPVTTVVITIEEDSTSPQGDDGLQSADRAEQVDKSTDGTASASLNKRDLPPQVAALLSQGQKTNTTTTTPAKRELPAQGAAPLNPGQKPNATATPAPPHLNQRDLPPQVVALLNQGQKPNTTATTPPPHLNQRDLPPPIAALLNQGQKPNATATPAPTPAKRELPAQGVTSLNPGQKPNATASNQPPHLNQRDLPPQVVALLNQGQKPNATTTPPPTPAKRDLPPQVVALLNQGQQKPNATATTQPPHTTAPPPVKRELPAQGAAAPVIQGQKPNATATTQPPHLTQRDLPPQVVALLNQGQQKPNATATPTPSQLNQGDFPVPVSAAPNQGQKSNPPGGRRSLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M993</accession>
    <name>A0A5B0M993_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_003497</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M993"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050295">
        <property type="term" value="F:steryl-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904462">
        <property type="term" value="P:ergosteryl 3-beta-D-glucoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041036">
        <property type="entry name" value="GH5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF5">
        <property type="entry name" value="ERGOSTERYL-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18564">
        <property type="entry name" value="Glyco_hydro_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="700"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="5">
        <location>
            <begin position="83"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5 C-terminal" evidence="6">
        <location>
            <begin position="583"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1073201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="721" mass="82001" checksum="D79AD7FA1A1A9192" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0M9J6</accession>
    <name>A0A5B0M9J6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_006994</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000158">
        <property type="protein sequence ID" value="KAA1073271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0M9J6"/>
    <dbReference type="OrthoDB" id="10287768at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1073271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="56" mass="6097" checksum="51585BFD7CD40698" modified="2019-11-13" version="1">MAAQQGFRTANIRTDVVIHIAATLTVAQRGMTLGKSATLVKLKQRLQHLHGNDESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MBF0</accession>
    <name>A0A5B0MBF0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007210</name>
        <name evidence="2" type="ORF">PGTUg99_027709</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000473">
        <property type="protein sequence ID" value="KAA1074165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MBF0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1090620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="65" mass="7179" checksum="C08766FCC6677610" modified="2019-11-13" version="1">MSQRLSVLPNMTTKITNSAQLRNLTPALGKLLGVQSSLLDRADEHCSASEQHKENLEKSSIMENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MDV8</accession>
    <name>A0A5B0MDV8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_050299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MDV8"/>
    <dbReference type="OrthoDB" id="2506095at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006912">
        <property type="entry name" value="Harbinger_derived_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150:SF5">
        <property type="entry name" value="OS07G0546750 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150">
        <property type="entry name" value="OS12G0169200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04827">
        <property type="entry name" value="Plant_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1074419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="51169" checksum="432CA12B25775391" modified="2019-11-13" version="1">MDERSLIRYHIELGRQAVEELLDDDQAVQNLIEELESESDDDELEPEERRRMPNKDRDRYDFHDKLMKDYIVENATYERQFSRRFRLDKEVFMRILQDLTANYTFFVQRPDCTGKLGLSPEQKLTAVLRQLGYGIALDATDEYCRLGETTARNNMSMFCTAIQELYGPIYLRTPTEDDLKRILSQNAARGFPGCLGSLDCMHWGWKNCPTAYARQFKGKEKGPTVVLEAVATKDTWIWHTFFGTPGSLNDINVLERSPLFQSSLAGNSWSVEFEVENNHYKNGYYLVDGIYPSWSTLIKSKGLQPTREARYYTKCQEAMRKDVERAFGILKARWKILTRPALQWYPGDLAAIMKTCIILHNMMVEYRYPPRPFDLLDSFQLIPPRPNPLSHREQLIRSIEMKSPAIHDNLIRDLIAHCWKLSGKTGDSSDSDLPEESDVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-05-03" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MEE1</accession>
    <name>A0A5B0MEE1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_007859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MEE1"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1074496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="217" mass="24224" checksum="A76ABB7930D0E362" modified="2019-11-13" version="1" fragment="single">MTLMTGVCWNFCALCALCAIRSAILSLHSLHSYRFSPQSSDFFFKQRLNFILVKRLASFLTRHRLLNNSVSKLVIASRSFQHNRNRPNQLRQNHKSNVLSKQYLRWALLSLSRLPPPAVSDTSSSILSSEISKTNPLLGLSSPSLSTIIYLLNNSSFHFHSTTLLLFPLSLHSLSHLPTCSLPLATISIHIKLNSASSHPYSADSLSRNTHLATANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MFS5</accession>
    <name>A0A5B0MFS5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Threonine aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">GLY1_2</name>
        <name evidence="7" type="ORF">PGT21_023648</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1074874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MFS5"/>
    <dbReference type="OrthoDB" id="10261951at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06502">
        <property type="entry name" value="TA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000030">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041359">
        <property type="entry name" value="GntG_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="L-THREONINE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="6">
        <location>
            <begin position="76"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1074874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="435" mass="48111" checksum="28F5154AA2213B33" modified="2019-11-13" version="1">MAIDCEAGTQTVSEIANELSSHLSIQNTNGQVNHGQANGKKNPLKTNNSYVQLNLPTGGYREPESNEEKRRLISRDFRSDTMTAPTEEMFEFMKNASIGDDVYGEDAMTNRFQEKIAQLTGKEAAAFCVSGTMTNQLAIRTWLTQPPHSVLCDSRAHIHNYEAGGIAFHSQATTIPVKPKNEHTLTLEDVQESIILGDDIHYAPTKLVCLENTIRGLVHPLEEMRRIRDFCHENDVITHCDGARIWEASAKTGHSIAELCEPFDSVSLCLSKGLGAPIGSVLVGPHEFIKKVRWFRKMFGGGIRQCGSIVAAADFCVTSNFPRLAETHRLARYLADQLVALGVRIAFPVETNHVFIDPSPIGIEIEELSRRALEELQIRISGARLIVHIQVSRQAVEELVGLVRRMKSSSAPSDSVSEEDEPGLSILTMSTTHRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIM8</accession>
    <name>A0A5B0MIM8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">methylmalonate-semialdehyde dehydrogenase (CoA acylating)</fullName>
            <ecNumber evidence="2">1.2.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_032565</name>
        <name evidence="6" type="ORF">PGTUg99_033794</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.27" evidence="2"/>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1076033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIM8"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000003">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase [acylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="58"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1076033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1091389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="541" mass="58790" checksum="4F2FE045C4F96BA4" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MIP8</accession>
    <name>A0A5B0MIP8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030620</name>
        <name evidence="2" type="ORF">PGTUg99_030293</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000471">
        <property type="protein sequence ID" value="KAA1075946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MIP8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1091298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="76" mass="8430" checksum="AB731BD913DC40C9" modified="2019-11-13" version="1">MLNGNTNVSLVPMGNGKKQVILYIGGQVRSRDQLQVVWRRPAPSPPVQRAFPGPAPLPTERERGLAQTDILKIRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MKM5</accession>
    <name>A0A5B0MKM5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_009183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MKM5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="95" mass="10378" checksum="40123E5BE85A8115" modified="2019-11-13" version="1">MTRQNDVSGLVPTIGEYSPAKISDSYVKGLENMMSDFDDCSSPPKLNRLLPTAMVDWKDDDPAESSVTKSTHEPASLSDSESEATNIIQKIELSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPA6</accession>
    <name>A0A5B0MPA6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_022513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1077866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPA6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022684057" evidence="1">
        <location>
            <begin position="25"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1077866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="79" mass="9188" checksum="783778F5DA1C136D" modified="2019-11-13" version="1">MQFNFLAALTFITLLGALPYITQSQNVPNDYMIKESVDRSKNYVFCSPQCGRKGCLQFEPNLPRPISRENYNHDLRLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MPG6</accession>
    <name>A0A5B0MPG6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000144">
        <property type="protein sequence ID" value="KAA1078392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MPG6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1078392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="64" mass="7545" checksum="4A4B81799CE0094A" modified="2019-11-13" version="1">MLSEIPTFFETRFEAVHPVSSLEDCYKLKFGKEKQVADSQIRSPNVEWESFFQMDGRLWESAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MR95</accession>
    <name>A0A5B0MR95_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012808</name>
        <name evidence="3" type="ORF">PGTUg99_027065</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="KAA1088758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MR95"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1079481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1088758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="79" mass="8864" checksum="38E206E0AB9C5AE6" modified="2019-11-13" version="1">MKRMRCVHRYRIASGVYRIASQDAMRGPASHRPHRHNRCGESASHRFSDAMSDTWLAMPMLSAGIGSTSLRVDSESESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MV34</accession>
    <name>A0A5B0MV34_PUCGR</name>
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        <recommendedName>
            <fullName evidence="2">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_026659</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000132">
        <property type="protein sequence ID" value="KAA1079888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MV34"/>
    <dbReference type="OrthoDB" id="10264848at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:small GTPase binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
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    </dbReference>
    <dbReference type="SMART" id="SM00167">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="2">
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            <begin position="378"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="673"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="833"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1079888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="992" mass="108079" checksum="F53EEDF6454C83CA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MVB5</accession>
    <name>A0A5B0MVB5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MVB5"/>
    <dbReference type="OrthoDB" id="2506685at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="515"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1080572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="61099" checksum="6EEB61CDA61B1098" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MWI6</accession>
    <name>A0A5B0MWI6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_030984</name>
        <name evidence="4" type="ORF">PGTUg99_032310</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000104">
        <property type="protein sequence ID" value="KAA1131349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MWI6"/>
    <dbReference type="OrthoDB" id="2506734at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1081187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1131349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="256" mass="26746" checksum="5BD1AFFC7F1CE1CE" modified="2019-11-13" version="1">MVASPDNQSGQWNGDTSGGNFTDTNTPNSVGSSTVGADGIPPISSATTSSSLPATPGLPFDTTTSSLLSSTSDSPLNTINSMPDPMATITAPSNMNSSEIASSSLPSSSAIPEPLNPSVMGHVELNAGMWQQPGIIIGVACSALGFLCIIIGLAYHLYRRRATRRPIRGLRVVSGFDFNGGGEDDYSTYNIWDPSPIGMEATDKGEKAGFEHEGSLTIMSSIRNSTATTYSNADDDEQSYPSSTQSFPIHEKGIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MXA2</accession>
    <name>A0A5B0MXA2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_025135</name>
        <name evidence="2" type="ORF">PGTUg99_014448</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1080906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000013">
        <property type="protein sequence ID" value="KAA1137054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MXA2"/>
    <dbReference type="OrthoDB" id="2496590at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1080906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1137054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="407" mass="46842" checksum="B52DC317F4B277BC" modified="2019-11-13" version="1">MFTKSPQEIEQELQAGRDLIASLQIKSEPPASPLQPSKVWKQSAATRLAFIKAFKEAIPLIENGPEGQAWQELLLRLGGQAHDYRVERSLVPRLRQHLVEISDVFVPARLQQVLSSENPASQVGLMKLVATEINQLKSSVTSPCSNQTSKISTYALDPYYFPDLPWEVDQIRDGIIEAFKEYIKTLKAKEPPSGDMRKPSLRELLESVDRMHGDFEDPRRVLKAKWKYVAREIEIMHMTVGNCLKPRAACKNTNPNGRVDEPMVHPWPSLEEMNKADIQTFQAYIPLLKICRLLLVKLCKTPASDQLLIYKMSLADLKEFYNKTEGLEEDLRKFTTMILERLTSRAILQLDAFLVWFNYIIKTVETHLATLDSPADRAHVRKSREWCLMWKIQYQIAIRNFAPKRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0MYR5</accession>
    <name>A0A5B0MYR5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_032535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000131">
        <property type="protein sequence ID" value="KAA1081264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0MYR5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1081264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="122" mass="13074" checksum="DF5C9D7AA58FDA34" modified="2019-11-13" version="1">MSGSLTSNKSEQANLLFQGTSVVDDSCDMEDVPPETKSRFILYPEITEEAADSNTFPVRGGPPQQSSATTIILRKFNKPKYTSLEMAPLHPDIRADADIRLPFQGEATSAPATATAGGYPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N2Q8</accession>
    <name>A0A5B0N2Q8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016872</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N2Q8"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1082856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="67" mass="7656" checksum="DFF9EC3771BE4AEE" modified="2019-11-13" version="1">MKVWFNPSLEVSELPIKRFFRTAISNSLEFDKNGARHPSAWSTSTVSQQRHYSHGHRFPTGLARSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N3X7</accession>
    <name>A0A5B0N3X7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_011913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N3X7"/>
    <dbReference type="OrthoDB" id="2516745at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1083931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="103" mass="10796" checksum="99B1A5F0CA9D29F5" modified="2019-11-13" version="1">MEPTKRSSTPDAGQQLTAAPTQSSTPDAGQQLIVTPKQSSTPDAGQQLTAAPKQSSTPESDNGHCVVSTSAEQLLLVAAEQPAERRAARKSSTNKAFPPQQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N4J3</accession>
    <name>A0A5B0N4J3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">C-4 sterol methyl oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ERG25_1</name>
        <name evidence="6" type="ORF">PGT21_003502</name>
        <name evidence="7" type="ORF">PGTUg99_012703</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000209">
        <property type="protein sequence ID" value="KAA1123352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0N4J3"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="5">
        <location>
            <begin position="159"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1083672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1123352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="318" mass="37587" checksum="596983DE471257DA" modified="2019-11-13" version="1">MATQFQQILNHTSSLLPSIQSWEEFRTQNKIEQNHLYSGSDLSSLSIFEQLWLRWYLYFPNPVIATGIMSFLVHELFYFGRCIPWIIVGKIRAFDKYKLQPNKIPSREDQWKCTKYVLWTHFTVEIGQIWGFHPLAEYFGMATHSVPFPSIFTMAYQIALFFVFEDFFHYWAHRALHQGQLYKKIHKLHHEFSAPFGLAAEYAHPLEILILGTGTIGGPLLWCVLSKGNLHILTMYIWIVLRLFQAVDAHSGYDFPWSLRNILPFWSGADHHDYHHEKFVGCYSTSFRWMDHLFGTDKGYHEYRKKQKLAKLNKEKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0N6F0</accession>
    <name>A0A5B0N6F0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_035866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1087614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="176" mass="18645" checksum="FC1830EF13093FD4" modified="2019-11-13" version="1">MSSSFPYSEYPFLCSQDPNCHDQPTGLPDYARGLIGVFVSLFGIAMIFSIWKRSRRSSASNGPAMDKEANVGAKKNDHDERERESPSDSDSPSDAERNNDNHTSGHEMATPHTPGDGSNDGNSSACGGWFSGISWFDEGGRAGGDSTDGGDWTGSHRVERAGDNSFGDGGGASSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NH87</accession>
    <name>A0A5B0NH87_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Syntaxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_025176</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Essential for proper morphogenesis of the vacuole. May exist as structural reinforcement on the surface of the vacuolar membrane and be required for maintenance against rupture by osmotic pressure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000105">
        <property type="protein sequence ID" value="KAA1087199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NH87"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097576">
        <property type="term" value="P:vacuole fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15858">
        <property type="entry name" value="SNARE_VAM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000058">
        <property type="entry name" value="VAM7p Vacuolar SNARE protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="PX" evidence="8">
        <location>
            <begin position="4"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="7">
        <location>
            <begin position="347"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="279"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1087199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="46053" checksum="7B55285070205056" modified="2019-11-13" version="1">MEVQSIAVKSFEIIKPKDSSKPYVLYTIEIGTKLKSYTICRRYSHFERLSEDLKLELDSAVSRRWLPILPPKSTSLVGYLTGSGSLINQPSKIAERQAGLERWLKTVIVHKELKDKTSKSRALMDFLEIPSSLNSTSSSATHKSSHMDDHSANPFTSQSWLAEHNEIRASVREMYDLLSRRDELLHQSTSTSVTLTRLNQINVDGKRNLENITARLGRLTESLKLFTLPDTPKGRGTLTEGETSRRMQLISTLQDDCERLSKKILARAEIGTGIRQANQVLSSESASRDRAELLGTHSNGSNSRPTTRVLGGGGGPTGSASRNLPAEETVETRQRDNRQLMDHQLNHVIAQTQDDKLKWLTQILSRQKLLGLMINQELAEQNEILDDLDRDVDSASRKLKDATKKINRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NHZ6</accession>
    <name>A0A5B0NHZ6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_001554</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NHZ6"/>
    <dbReference type="OrthoDB" id="2497674at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1088945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="423" mass="50303" checksum="53EA4E57447C5C73" modified="2019-11-13" version="1">MDELAEANRKSLSARDPSQSSLETLDGLAYVADSYEIWEPFLRESKNETWDKISTIKHGCDLMIDVFIDLQKHQIVPNDHLALFLNKENRGKLILAYAMGRFPRRTKQFARVPYVNFNLKLSLRENPSTRKMAGILKLLDQKTWQYLEFEHLQNQLWKEYPMIEYSSPDFNHLKLHFLTQASPHKELTGIEVEDLLEKLITHILKIELSGKDEFVHEKFVHYKQLYDMVGFLMEYNKHKISHEFKQKHSEDFKRIAVFEEAVGLVSSMILSVFIRLTDAENQQQEKFHHLDLLSDLNQNRAVSRFNQEGDIVGNELKKRFENLPNAKEYLESFLYRIKPTTSRIVLHKDLTKVDDSINKFKQQFKRYYIRIRNLIDENQACDQMFIKTIRNFTYYSANYVFEYEQRWGAMADEFMKEKANEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NID5</accession>
    <name>A0A5B0NID5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_003312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NID5"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022777593" evidence="1">
        <location>
            <begin position="27"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="64" mass="7057" checksum="51E76FF1DC60AA80" modified="2019-11-13" version="1">MTSSKIPFFLVALCTLGGSQIPLSKGMEVKGLGKYLADNRKIQTFSCYRELFEDSQPTPSPESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NJL6</accession>
    <name>A0A5B0NJL6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_002271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1088963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NJL6"/>
    <dbReference type="OrthoDB" id="2506660at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1088963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="546" mass="61976" checksum="DA484FBE1D5A6142" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NLP2</accession>
    <name>A0A5B0NLP2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Malate synthase</fullName>
            <ecNumber evidence="2 8">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGT21_005423</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the malate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="2 8"/>
    <dbReference type="EMBL" id="VSWC01000093">
        <property type="protein sequence ID" value="KAA1089060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NLP2"/>
    <dbReference type="OrthoDB" id="186072at2759"/>
    <dbReference type="UniPathway" id="UPA00703">
        <property type="reaction ID" value="UER00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00727">
        <property type="entry name" value="malate_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1220.12:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.360:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="entry name" value="Malate synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="entry name" value="Malate synthase, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019830">
        <property type="entry name" value="Malate_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048355">
        <property type="entry name" value="MS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048356">
        <property type="entry name" value="MS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902:SF1">
        <property type="entry name" value="MALATE SYNTHASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20659">
        <property type="entry name" value="MS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20656">
        <property type="entry name" value="MS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="MS_TIM-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="Malate synthase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00510">
        <property type="entry name" value="MALATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Malate synthase N-terminal" evidence="10">
        <location>
            <begin position="85"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase TIM barrel" evidence="9">
        <location>
            <begin position="225"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase C-terminal" evidence="11">
        <location>
            <begin position="475"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601465-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1089060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="605" mass="68028" checksum="2449FE084D197FBA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NN61</accession>
    <name>A0A5B0NN61_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Golgi apyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">YND1_2</name>
        <name evidence="7" type="ORF">PGT21_012230</name>
        <name evidence="8" type="ORF">PGTUg99_031657</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000171">
        <property type="protein sequence ID" value="KAA1070437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000404">
        <property type="protein sequence ID" value="KAA1089964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NN61"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046036">
        <property type="term" value="P:CTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006256">
        <property type="term" value="P:UDP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24039">
        <property type="entry name" value="ASKHA_NBD_YND1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF121">
        <property type="entry name" value="NUCLEOSIDE-DIPHOSPHATASE MIG-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="577"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="634"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="694"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="777"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="634"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="724"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="777"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="788"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="823"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="210"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1070437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1089964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="834" mass="92012" checksum="4FF1FA5AC4220EFC" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQD0</accession>
    <name>A0A5B0NQD0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_035092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQD0"/>
    <dbReference type="OrthoDB" id="2518389at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1091517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="111" mass="12481" checksum="D62BDBC11025E43D" modified="2019-11-13" version="1">MLSAAFLWKCQKNTTPPADDYLFLTPSQINTILHKGKASPATSLIYSADQLNEIAHSCLIDLQDYFNLYAARSPQEALACVYLVTRHQDHFSAFLKAVKLLIDRSIAKQHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NQW2</accession>
    <name>A0A5B0NQW2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_021845</name>
        <name evidence="3" type="ORF">PGTUg99_011945</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1091052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000178">
        <property type="protein sequence ID" value="KAA1125440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NQW2"/>
    <dbReference type="OrthoDB" id="2498281at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1091052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1125440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="285" mass="31546" checksum="3D72D935A10749AE" modified="2019-11-13" version="1">MTSRRKKIMVDPPPSSTTSSSTEESSDAPSPIGPSKRTSQANPPPATTNPTNLAVGGSRGRPDAATVTRVQTATTPATAPGPAHTNAVYHPPIVAPNPQHGHLAAPASGPINHYGPAPYHNPPNPAPIPANVGHHQANQLTHQPNQLNYQHHQTNHQPNQLTHQPNQLNHQPNQLNYQPNQLNHQPHQTNHQLNHQPHQPYHPPHPHPTYASEDQIELERYLRFVHVNPHSKVLQNALDELGITHYSFFEFFSVDELVDLGIRIAHARALLNGLKKFDRIRRMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NSU1</accession>
    <name>A0A5B0NSU1_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vacuolar protein sorting-associated protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">VPS20_1</name>
        <name evidence="8" type="ORF">PGT21_018196</name>
        <name evidence="9" type="ORF">PGTUg99_009034</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">21-0</strain>
            <strain evidence="9 11">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000092">
        <property type="protein sequence ID" value="KAA1090929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000177">
        <property type="protein sequence ID" value="KAA1125501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NSU1"/>
    <dbReference type="OrthoDB" id="441172at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="170"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1090929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1125501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="222" mass="25203" checksum="971D9D77238B365C" modified="2019-11-13" version="1">MGQGRSSQARITNQDRAILDMKLQRDKLKQYQKKIETVLKLEEEVAKQCLLANNKPKALIALRKKKFQENLLSKTDQQLETLQNLVSSVEFSLIEKDVLFGLNQGNQILKEIHKELNIENVEKLMSETADSIAYQNEIGELLMSQMTNEEEEEVHKELEILQSLNQKVTDPQLVPHDEPPVSLSLPDVPTTVLPTKTQTASPAEEPPKVDSTRVREREAIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NTV3</accession>
    <name>A0A5B0NTV3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_012404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1092737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NTV3"/>
    <dbReference type="OrthoDB" id="2499206at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1092737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="175" mass="19726" checksum="CE7AACFB79D8CC9A" modified="2019-11-13" version="1">MENRLFSDNRPAAMSLMRGAGFVILLRIMLILLMIHLIPSARLANVVGPQQCTNGFVLASYHSDSVSCKTSQNVIYDCRVSKCFATSDTSQHGKPYSEFVFEKCHRIDASDHPTKSTSTIHPAEFYPTYNEKNWLEIHGSPPLQPGRRRYQCFTSEAQKLNTNRPWCVGCSLPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0NZ82</accession>
    <name>A0A5B0NZ82_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGT21_026701</name>
        <name evidence="10" type="ORF">PGTUg99_016090</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11 12" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">21-0</strain>
            <strain evidence="10 12">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFP11/STIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1128968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0NZ82"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022783">
        <property type="entry name" value="GCFC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022159">
        <property type="entry name" value="STIP/TFIP11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045211">
        <property type="entry name" value="TFP11/STIP/Ntr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329:SF1">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07842">
        <property type="entry name" value="GCFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12457">
        <property type="entry name" value="TIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="G-patch" evidence="8">
        <location>
            <begin position="268"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1003"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAA1094617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1128968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="1042" mass="117260" checksum="7D02CA4805364DA9" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4G6</accession>
    <name>A0A5B0P4G6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_013836</name>
        <name evidence="3" type="ORF">PGTUg99_036840</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000102">
        <property type="protein sequence ID" value="KAA1132078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4G6"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="1">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1132078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="208" mass="22784" checksum="AB58594668084974" modified="2019-11-13" version="1">MAEVVDELRILAHDVYGSSSKTTGPSAPISSRCADSENKVSVEDAIEVYLKQGFKPEQLSLAIPGFGKSWTLAQPELTPKVVGNYTTYYYQNYTGIPPGGKWDDEPGVDDCGKKTGWGGIWLVKELVQDGFLSKDEMHGMKGFTRYYDACSGQPFLASNTTMISYDDTQSTLAKVKYAKSKGLGGVVFFDTMGPRDKTVKAARKALYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P4Z3</accession>
    <name>A0A5B0P4Z3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P4Z3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="432" mass="47468" checksum="8C055A78C341F6FD" modified="2019-11-13" version="1">MSPSTRSGKTYSEASSVTSSRRSSTTRSVHEGKRQPRSIARKDGDPLEAGQINPTFESQLKPSSVEGNKPNVETNLAEAVRLTRQEKDPIDDSQEIAGENRRKTIGTIPADESTGKSNPNPAIIREGQEETSYSGGHTNVNLVETTNADHFRSTGRHPSHSTELPSGIEGPQDRPEPTVGLDGYRPTGGSRPERRSGSFNGYRTNESVCSSDDSLDIFNQTLTTDNFRAKRGRRPSLQNLPPAVSHVQNGEESKPARRRNESADRVPALVPQHIQETDLAVCSLHQQRMESIQGGQIGQITSEVGRTSAADGKRVSTLRELHLLQQPTREKASRDRPQQDRQDTEARHLLARNNGGSQSLTASQSSDIRRVTQGGGSLQAPQERLDLQLVHTTLASLHTTLIDEIHARFVNFDEKLDILYSSNNLIGKQENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P736</accession>
    <name>A0A5B0P736_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribonucleotide-diphosphate reductase (RNR), small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">RNR2_2</name>
        <name evidence="2" type="ORF">PGT21_003853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000067">
        <property type="protein sequence ID" value="KAA1096058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P736"/>
    <dbReference type="OrthoDB" id="10248373at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF18">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1096058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="226" mass="25749" checksum="E8BB827CBF44D625" modified="2019-11-13" version="1">MRMVIRLSCTAQPRRPVAQLSWKHSAVTAQGPATSIQKPLLHSSPRLQPNDPTNIRFQLTPTTTPSHPDNTHSYINISINHDVSLRTRAKNLSLTPSQLNPLIISVAAPLSTNRFVGNLDITSDDQEPLLKEDGHRFVLFPIKLHEIWASYKRAEASFWTAEEIDLSKDMHDWDNKLNADEKHFHQTRPRPSSPPPMGSSNENLIERFQWRGFQWPKHVASTDSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0P8M4</accession>
    <name>A0A5B0P8M4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023983</name>
        <name evidence="3" type="ORF">PGTUg99_036016</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1097927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000270">
        <property type="protein sequence ID" value="KAA1117116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0P8M4"/>
    <dbReference type="OrthoDB" id="2498491at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137685" evidence="1">
        <location>
            <begin position="21"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1097927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="218" mass="23845" checksum="679734C23BA8679A" modified="2019-11-13" version="1">MVAFAHLITLLSVLVAMSRADEVAQAKPEQVDDKWYYGRFRSAGYWGLSGMSLYSTYPSLYSNYYSLLNSYCYGLGTFPYNYNLINGYFAKATDAEKSVSRRAISLDAEQLVRRDAADSFTCTTHDAAPQTFSAKECVAAAQQLSEKKVSTASHGGCKVALANAKEKVAPGHISLKDLETAVHSILSGCSNAQNKEVANNKPEKNIDEKQVVMLITKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PEZ0</accession>
    <name>A0A5B0PEZ0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_031065</name>
        <name evidence="4" type="ORF">PGTUg99_019965</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1084572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000342">
        <property type="protein sequence ID" value="KAA1099194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PEZ0"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137761" evidence="2">
        <location>
            <begin position="21"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1084572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1099194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="138" mass="15261" checksum="21250011C915248A" modified="2019-11-13" version="1">MQFSKLLVILISVTSSLVLAKADTKYIFTCDHQTFKGKPAPQAVCGRLIDFPDQPTKAPTDFNLVEPISGGSKSKQNPKSNSNRNSNSKPEPIVNKLVCADYRYLAMCCHPNTVNFEHKPSTLSIPVINDRCTNPPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGE2</accession>
    <name>A0A5B0PGE2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_016071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGE2"/>
    <dbReference type="OrthoDB" id="168171at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="205" mass="24125" checksum="7487BA4FAB52B767" modified="2019-11-13" version="1">MPRFRSDMTIWGRMLDHFHIRHHHPSTKHMSSSPLPQRVNRREAGREHQWDWDAIDGGPSSLLILLNWLTKPGNYSRWNTPGNCDHKLICMEILELMENEGITHQHVWGINHRIQLLRRSYNTARDFIAHAQHKPNIQDMIITRHARWVCPHWDLLLYNLVDQEGMLLASKAVQPCRPSLWIKTPQKAPQTPKPIMNPLKHRSHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PGH7</accession>
    <name>A0A5B0PGH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000054">
        <property type="protein sequence ID" value="KAA1099942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PGH7"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1099942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="165" mass="17782" checksum="62ED4A35D9638708" modified="2019-11-13" version="1">MFSSSTWNGKWLELSSSNFFSSSSSVSSWTQLIQLSQLKSALRPSSVSSRTQLSQLLNPAQSALGPSSVSSWTQLSQLSDPAQSALKPSSVSSQTQLIQLIQLIQLKNPAHPAQKPALIPKPNPKPNPNPKPTLNLTLTLNLTLNLTLNLTPNPKPLNLTPNPNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PH55</accession>
    <name>A0A5B0PH55_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_036121</name>
        <name evidence="3" type="ORF">PGTUg99_021723</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000066">
        <property type="protein sequence ID" value="KAA1098516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000341">
        <property type="protein sequence ID" value="KAA1100353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PH55"/>
    <dbReference type="OrthoDB" id="8058986at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1098516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1100353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="257" mass="28794" checksum="5F101CF02FB61A9E" modified="2019-11-13" version="1">MEAQNEGVGVPYPPNREREEGRDPSPEPCEAERRLAVETRDHVSSIVEATAHRLEDFYDDDARRLRGYTMRYLDNFEHAVLNMVDQRIKTSETAIERRVSARVKQTLSSRTSLTNQPSLGAQPHLGAQPSLGIQPSLGVQPSLADQHIPGDQPGLAAQTGLGNEPGHASTTGRADGTRLEDLCDRHYRTLRSRLDGILRLEMQEEKKSIRERLQKQQEFIFDVIYSQAKSPRERHVDAESSRASRTVEEGSSSGQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLP1</accession>
    <name>A0A5B0PLP1_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_004993</name>
        <name evidence="5" type="ORF">PGTUg99_004950</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="5 7">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000337">
        <property type="protein sequence ID" value="KAA1102515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLP1"/>
    <dbReference type="OrthoDB" id="6159137at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="15"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1064488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1102515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="127" mass="13924" checksum="49262673419FFC3F" modified="2019-11-13" version="1">MPLIDRIMPAAQQTTKINIANLAEGTSLADVMTSFSQFGKIMRCSLKQHYDGQDTSVSATIEFLSYTSAVSAVEELDGATADGLCLHVKLVRVVRPPVHIATKEDSTKQEVQDESPLLHRISKRPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PLQ7</accession>
    <name>A0A5B0PLQ7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_034179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000053">
        <property type="protein sequence ID" value="KAA1101956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PLQ7"/>
    <dbReference type="OrthoDB" id="2509241at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1101956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="387" mass="43667" checksum="19375617277ED9E5" modified="2019-11-13" version="1">MADAEGSSRASVLQALVDLSVKYQHAADNAASDWQDEEREPTEDDRKRKIEVFDKLHSSLLPSIKHHLSCLVTSLDLEHRPSNYPTPNLGLTLETLSSLDHTLDQIIVDFGLLNVPLPPVAHDHPLDRCKRFRSDQLISNISDLIQDPIRSTFLYCSDFIQLSELSTDQPESIGLQTSVSNSAETVLDGITEATNLVTKILRFSQLSDLDILQEQWQEKAESLDGILEGLTELTNSPIGHRRPLSAVRKSMLNETRLDITLVKLLRTLYDKVSKPTIKKLAFKLDPDVDSESLSIMHTAPETTDGSLLIHLHFLLENFKNDQSNGETADRKISKSRISRRVESMLLPIGLYLIPLPSSVDHHSSPARDFKAWLIMWQTLWHTAINRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PSH7</accession>
    <name>A0A5B0PSH7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_030397</name>
        <name evidence="3" type="ORF">PGTUg99_009648</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
            <strain evidence="3 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000414">
        <property type="protein sequence ID" value="KAA1086051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PSH7"/>
    <dbReference type="OrthoDB" id="2514325at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474354" evidence="2">
        <location>
            <begin position="18"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1086051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1104715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="319" mass="33609" checksum="8D4EB11A603EE5DA" modified="2019-11-13" version="1">MLSAVYVACLLAASAIASPVQHNPRYVAGSYSQDSAHSNEDHARGASSTPFSSSSYSSDRSNSDASHKSGSYFNGGSPYLGGGAAGFGGYGNYLNGGYGAGGYNGAASAYNSASAYNSASAYNTASGYNGIGSNGYGIGNYDPSMYAGGASNFGSANSFSSSQSQESYSMSTVNSAFSSLNSYLESMNSRLISGQITQQAIYQVVQQITQYFESAMSQATYCGECVNGQSQFSSVASSSFSQFTHFMNQVERQFGGSIFDQFSNPMSRLSTSIQSFFHRASSSSFSMTSQSFIPSDFTPLAQRLMPDVVPSLSQFQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PU40</accession>
    <name>A0A5B0PU40_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_018581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000042">
        <property type="protein sequence ID" value="KAA1103459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PU40"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1103459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="159" mass="17276" checksum="94936D23E69EC977" modified="2019-11-13" version="1">MVGPDRASAVSMTARSTQPHAQQGIDRWVNPACMKTGGMTAGHADEVYMWRADDSVIPTVGRPTDPRRPAVNNLTTAGRIEKSRSATKAQPEFADTMAPRTPVKYHGFAPEAGRLKSIFRGDSRFQMGDIAMSTLSAPLTRGACDSDSQLWLRGRDGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PUR9</accession>
    <name>A0A5B0PUR9_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_020696</name>
        <name evidence="2" type="ORF">PGTUg99_031918</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000505">
        <property type="protein sequence ID" value="KAA1068573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000041">
        <property type="protein sequence ID" value="KAA1104358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PUR9"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036137828" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1068573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1104358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="10692" checksum="8DC9F2171A7F4541" modified="2019-11-13" version="1">MKYLLSASILFLGIYGPPNSQAKLFDCPVDRPNGACSSVDLFSATANWQLTGAECPNKAKPRTFKFCDPSVSTNCCEPGVPMSVSTISISSSTHALSIDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PWV4</accession>
    <name>A0A5B0PWV4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RPS12</name>
        <name evidence="7" type="ORF">PGT21_004148</name>
        <name evidence="6" type="ORF">PGTUg99_020541</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="6 9">Ug99</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000406">
        <property type="protein sequence ID" value="KAA1088373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PWV4"/>
    <dbReference type="OrthoDB" id="10249311at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000530">
        <property type="entry name" value="Ribosomal_eS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047860">
        <property type="entry name" value="Ribosomal_eS12_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11843">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00972">
        <property type="entry name" value="RIBSOMALS12E"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01189">
        <property type="entry name" value="RIBOSOMAL_S12E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="34"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1088373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1105360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="149" mass="16062" checksum="47B5FFDB7A55C9BC" modified="2019-11-13" version="1">MSDVEDAPVDVAAEEVEVTQDADAPKGGQMSVEDALKKVLKTALVHDGLARGLREAAKALDKRQAHLCVLNEGCTEEAYVKLIEALCSEHKINLIKVGDGKLLGEWCGLCKIDRDGTARKVVSCSCVVVKEFGQESEALNVLLDHFGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PYQ4</accession>
    <name>A0A5B0PYQ4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Peroxin-10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">PEX10_1</name>
        <name evidence="23" type="synonym">PEX10_2</name>
        <name evidence="22" type="ORF">PGT21_023576</name>
        <name evidence="23" type="ORF">PGTUg99_027743</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 24 25" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">21-0</strain>
            <strain evidence="23 25">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000240">
        <property type="protein sequence ID" value="KAA1121026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PYQ4"/>
    <dbReference type="OrthoDB" id="2504210at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16527">
        <property type="entry name" value="RING-HC_PEX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001407">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350:SF0">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="21">
        <location>
            <begin position="383"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAA1105899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAA1121026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="435" mass="49677" checksum="BF8517488535B64B" modified="2019-11-13" version="1">MTNQEPPPPPSSSSLEPAQSSSSSTHQSTGAPNVPLSAEENSKLLRIWSKNLRFPSAAQPEIIRADQKDAYFINALFDQIEPLLRAAKGSRWVNNNVTRLQDASKLIYLSLTTLPGSQTLGEEYCDIIQFDAFNNTLPALYRRAILIIIEVFSPRLLSKLYERLRQHITRMNDRDSTNNEDHPSSEASHQQHESRLGSLKRKICAWLAYLPNTLDRSTLDSCNALHLSIFYLTGRYFTWSKRFSGITYISDRLRPLRADGLGRESPPSYEILGVLMVIQLVVKVVGTHRQARRRREQLRLASEPVAILDKKESDPKQSNRVLTVDGRLIDEMILEPEDEEEEEEGLDEESDEKDKLDHQKLVDECPEGVLIDDLSDQVTTRRCTLCLGPRKDQTSLECGHLFCWRCLVSWIREKPECPLCRHSVHLAELLPLYNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZI5</accession>
    <name>A0A5B0PZI5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mRNA m(6)A methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.348</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_033279</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(6)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55584"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12417"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
            <dbReference type="EC" id="2.1.1.348"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.348" evidence="1"/>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZI5"/>
    <dbReference type="OrthoDB" id="10262526at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001734">
        <property type="term" value="F:mRNA m(6)A methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF7">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1106325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="367" mass="42024" checksum="3918B3446E87AF53" modified="2019-11-13" version="1">MAVPTQAPQNVDPNYVDLCPYTSRKHCRQQSQAGCTKVHYKPDIQPHTDLSLGDCSYLNTCHRMDTCRYLHWMVEDPLAFPTPAPTSESDALVKSQTASSSKLFPPQWVNCDLRHLDVGILGKFNVLMMDPPWDIHMSLPYGTMTDDEMLKMPVPSLQESGGLIFVWVTGRALELGRDCMRTWGYERIEEIVWVKTNQLQRLIRTGRTGHYLNHSKEHCLVGFKRPADKVGEGSMTKEEIREALSWVKSGIDTDVIVAEVRETSRKPDEIYEMIERACPGGRKLELFGRAYNFRPGWITLGNQLETDCLYEPETVERFNQISPHPISRMFLYICLLGMAHTGRVVWSSASFYRYPERQIKQAILPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0PZK2</accession>
    <name>A0A5B0PZK2_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGT21_050156</name>
        <name evidence="6" type="ORF">PGTUg99_050169</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7 8" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">21-0</strain>
            <strain evidence="6 8">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1106350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000305">
        <property type="protein sequence ID" value="KAA1109404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0PZK2"/>
    <dbReference type="OrthoDB" id="2508351at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="243"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="290"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAA1106350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1109404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="311" mass="34085" checksum="5A5C775726FE09E6" modified="2019-11-13" version="1">MPACSTQNPSALIGNVPVLDGNSFGFPAWRTRLEELFAIQGVHNIVTGKLVRPNIDPKDSKDVPAKPVTQGSQKVYYPEESATNWDTLSDVARATLKMTLSVDLAIRYKEVKPVNVLFKTICDAYEKNTRARRMMLQDSFWCARHDPNQPIAKWIAKIRNAASDLKSVKLTPADQQVCDRLLRGLDDSWKPIQDHLVYSPNEISLDDAIGALEAHEVSMQVSFENGSDAVVSAAIAKKKKAGCWSCGQIGHHLSACPNPPLKNKTKAKANAVEARAGATSVVQLGGGGPSDDDEEYEEEDDFDPEINVVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q1P8</accession>
    <name>A0A5B0Q1P8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_003310</name>
        <name evidence="4" type="ORF">PGTUg99_005732</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000010">
        <property type="protein sequence ID" value="KAA1137221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q1P8"/>
    <dbReference type="OrthoDB" id="10297344at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5033474517" evidence="2">
        <location>
            <begin position="19"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1107125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1137221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="379" mass="43217" checksum="8067CF06926EC7DB" modified="2019-11-13" version="1">MSLTKCMVITTLIAGVWGVKVHQLAVDLKFSQHNDHYMIFDTEPHKSPNFVKEQVILKTQSPPQSFSWWFPLGDCIRADRNMIEKKSKNVVPLEEKRNDEICITNNLRIEKVKNYGRTPFSVYNGVPEEPIMNFEKDGESELRWIPEKRFLLATNHLGAVLNEELYDFLVYPFLAAVKEGSNSMPTEYLYAETWQPSFREFILRVSNIGRTNHKSEAKYTMFRVDHGVTTNKSFSVGPRGQNGEMYARSITNKTQGKLRVIQTGIHEMNPENVLVGPYGSTRVLFDPSFPWVVVSNWGRISEKTYDCLVRIPLTKARQRRKTDNLQQPHISGSSRFQEDGTTNLNSPGKGSPSAISEFGKPTQDSSHQFEPVSQASSHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q2I3</accession>
    <name>A0A5B0Q2I3_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-rRNA-processing protein RIX1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGT21_016875</name>
        <name evidence="8" type="ORF">PGTUg99_029387</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9 10" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">21-0</strain>
            <strain evidence="8 10">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RIX1/PELP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q2I3"/>
    <dbReference type="OrthoDB" id="20900at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012583">
        <property type="entry name" value="RIX1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105:SF1">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08167">
        <property type="entry name" value="RIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein RIX1 N-terminal" evidence="6">
        <location>
            <begin position="13"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="462"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="669"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="738"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="464"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="669"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="687"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="738"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="787"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="822"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="841"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1107510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAA1124687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="851" mass="93741" checksum="3C3EB5B776DE0F05" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6G6</accession>
    <name>A0A5B0Q6G6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_024985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0Q6G6"/>
    <dbReference type="OrthoDB" id="10588099at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1108768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="214" mass="23515" checksum="C5E924A7C94EF60F" modified="2019-11-13" version="1">MTTTTQLTWQTQASVAYTTVIWLSHCGDATAVALRECHSRATTTPKPNAATAGKTEAKSVPASDNHGQLTPEMLLQCPGKTHRLADLQESSPGWIKWTPGRILPQQAAGRYYLPTHESLPMRIPPRQAALRAGLGPAAGIHRMTIGCCFTCGTGINCSTTLTNNRRLACLNYELRLAAHPPWQTFHDWPVLSESRFFAVFFSNLEADWPVQSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q6R6</accession>
    <name>A0A5B0Q6R6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_026556</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1108823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="282973at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF34">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="697"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1108823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="760" mass="85862" checksum="20BBB3B855A54007" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q768</accession>
    <name>A0A5B0Q768_PUCGR</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OMA" id="DPNRANE"/>
    <dbReference type="OrthoDB" id="2507256at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        </location>
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        </location>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="480" mass="51687" checksum="FE9648FE4FBBBF34" modified="2019-11-13" version="1">MSVSQLDPSLMDLSPLGQTVAQSPSGGPNVSHLNLGTDSSPKGADPTDSPNQTQGVPSKKGPRPRRTKDEMILYRAEQDRVKKAKSLAKAKGKRGRKALPRGHLSRGSQRAPHADSHSSGPGSAPPGSQPPVSDANPPFIAEDYEHVCSYLEEESNYTQLYGDGSKTTVGTTKVTKAAAYDIFAIYVNDNSNRRLRLTGSQLRQRIDGYKKRFLKAKDWAENTGAGIEEGDDLPTLAELLEKKCPCYERMYGIFGQKANVTPLAQHDSGVGADLYPNSQGRPLNESQEIFFSGWDETQDDLPQDPLETPDDRVDESDLPPPLNLSGTALDASPCLMTHCSLACIGPPGSPLGTPDTGGGNASNSRSIGRRPFANQPSADASPGGPLREVPRPKTSLASAFESSNADKFSYLKQHMALEKAKEDNRLEWEKERFDKEITEAKNLAAARATLAQTKLAAAQDLLKSGTTASEVDALLKTIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0Q7Z1</accession>
    <name>A0A5B0Q7Z1_PUCGR</name>
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            <fullName evidence="3">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000028">
        <property type="protein sequence ID" value="KAA1109094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000324748">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SMAD/FHA domain"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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            <end position="707"/>
        </location>
    </feature>
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            <end position="146"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="450"/>
        </location>
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            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="642"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1109094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="712" mass="77097" checksum="2386BDC89CD69F15" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QBC7</accession>
    <name>A0A5B0QBC7_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QBC7"/>
    <dbReference type="OrthoDB" id="2499284at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022937759" evidence="1">
        <location>
            <begin position="23"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="717" mass="82355" checksum="D43FA2580721390C" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QDM6</accession>
    <name>A0A5B0QDM6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_000106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000026">
        <property type="protein sequence ID" value="KAA1111229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QDM6"/>
    <dbReference type="OrthoDB" id="2506246at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1111229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="569" mass="60272" checksum="5CF586D7FAEA1BEA" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QGE6</accession>
    <name>A0A5B0QGE6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_020826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1112043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QGE6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022889176" description="Secreted protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1112043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15677" checksum="DD38CBAB9909990F" modified="2019-11-13" version="1">MSTMNVCHRGSVAFVLVTLIITLYSGHVAAGTKQQCSYHFSPTSSKGKGASCMQNDKDDMLCVLDSCRNDANGLKWDLVRYTFCSHNGQITPEAVKPAQYFRRSKYVEVQIDGTDKWIFCPYATDSASSTDYQTTCSDCSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2022-12-14" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QIG3</accession>
    <name>A0A5B0QIG3_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_016336</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1112946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QIG3"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1112946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="137" mass="15422" checksum="6E4D12A63B5D4D86" modified="2019-11-13" version="1">MPPPRGARIHEPRSSILPIRRCWRATRAGPRPFREVYVSSPISQVSILHDEYTTIGFEQRNIDSIPRKCDVWTDLKVSLIDAVLQENGGMGDAFPRPPSAGRNAGNVLPLRTRKVISLNVNLSGDLSTQVFMYRPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ40</accession>
    <name>A0A5B0QJ40_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1113176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="2498581at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="792"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="799"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="814" mass="92218" checksum="9FC24E1F3C8EE8BD" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QJ78</accession>
    <name>A0A5B0QJ78_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_025770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000015">
        <property type="protein sequence ID" value="KAA1113228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QJ78"/>
    <dbReference type="OrthoDB" id="10249045at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000964">
        <property type="term" value="P:mitochondrial RNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013943">
        <property type="entry name" value="Pet127"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014:SF0">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08634">
        <property type="entry name" value="Pet127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1113228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="448" mass="50294" checksum="52EF7D7550A9BE26" modified="2019-11-13" version="1">MTQTLSQIYLMLNGNKPLNIGMLTANAASTKKKVEFTPGAKMPASIISNMIMKQNDIRFDSDKSFDTETDCPDSTILSSLGHVLEKFFVLPYEEFAKTVEEEREFTRSIEAETTGGTNLLMTRSQLGLLSSLFARNGTFDIKTRATIAIRHDPVEPCPFLEIPDKWMDVLSHTTIPNASSDSNIFQSLRWIKPLFGNSQEGDKVFKLCLGYMERILEDATLAYPETVTFELLLLPKATEIYSLRSLTLTLSPSHQARIVRGGGLEDKEFGVVSSEEDREKIDWSIFCQTFKYPRSEELDQLYRSIRRSKTANELQESPSSTSDDDLSPSSSSSPLGIITQDTDKPGLSYSHFRPHTGNLIQETEVSKLVKHLRSIAKDGWKFQHQPRRGSERQKVISLKPAEFSIQDRQPSLSNRLPSTSPPSSSSTSSASHSNSDWQNHLIAQERLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0QJZ3_PUCGR</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <end position="624"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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            <dbReference type="EMBL" id="KAA1113294.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="725" mass="78894" checksum="9B5E319FD5B71694" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
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    <name>A0A5B0QK36_PUCGR</name>
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
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                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
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        </location>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
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                <person name="Sperschneider J."/>
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                <person name="Nguyen-Phuc H."/>
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                <person name="Pretorius Z.A."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAA1114546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <source>
            <dbReference type="EMBL" id="KAA1114546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="74" mass="8240" checksum="03694DCF4F67A570" modified="2019-11-13" version="1">MYHDDNDPQEKIGWCSHPYTLVNIIGFPANQKFMGAAVAGSQLRVRSPDFCVSGQVTVATWDAHYQISAKLQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QMX9</accession>
    <name>A0A5B0QMX9_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
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                <person name="Silverstein K.A.T."/>
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                <person name="Pretorius Z.A."/>
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                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1114637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="879" mass="100658" checksum="07F00908D2CECFA2" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QPX0</accession>
    <name>A0A5B0QPX0_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_034169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
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            <taxon>Puccinia</taxon>
        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2518661at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="138"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1115248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="234" mass="27307" checksum="0FCEDD550CC98F50" modified="2019-11-13" version="1">MTSATLRSVFILILFSLLLLTSLITILLPSLIDNPSQNKYHKQARVIGVDMSTIRSIRWQRLNYYPNSPLYPTVPWNKDTDKPLRHPTAAEVSKACRIADRKYYLMNFGRVVLVDPSDEDSVIAVMEFTPWDQLTEIDKENLNFISTFLHQSKEFINPVSSSTRSWGGKMWGIGWRKSQDFMQIFGRYIKAFPPSKMEKFDNIFQQSKRLGEILGDYYKPQSQFLFTSSHFHHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QQW5</accession>
    <name>A0A5B0QQW5_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">pyridoxal 5'-phosphate synthase</fullName>
            <ecNumber evidence="4">1.4.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGT21_021473</name>
        <name evidence="12" type="ORF">PGTUg99_021057</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.5" evidence="4"/>
    <dbReference type="EMBL" id="VSWC01000080">
        <property type="protein sequence ID" value="KAA1093020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000272">
        <property type="protein sequence ID" value="KAA1115651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QQW5"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00304"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004733">
        <property type="term" value="F:pyridoxamine phosphate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.110.10:FF:000098">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01629">
        <property type="entry name" value="PdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000659">
        <property type="entry name" value="Pyridox_Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019740">
        <property type="entry name" value="Pyridox_Oxase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019576">
        <property type="entry name" value="Pyridoxamine_oxidase_dimer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00558">
        <property type="entry name" value="pdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004231">
        <property type="entry name" value="PRK05679.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851:SF0">
        <property type="entry name" value="PYRIDOXINE-5'-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851">
        <property type="entry name" value="PYRIDOXINE-5-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10590">
        <property type="entry name" value="PNP_phzG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01064">
        <property type="entry name" value="PYRIDOX_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="9">
        <location>
            <begin position="98"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridoxine 5'-phosphate oxidase dimerisation C-terminal" evidence="10">
        <location>
            <begin position="247"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1093020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1115651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="293" mass="33287" checksum="97AFF7E5DFEC6929" modified="2019-11-13" version="1">MFPATPLIGRLRPSTNRLTLRFISPVHHHHHHQPKTDQHARPGGGAGGGRRIRMSSTVTEITRHQQYHSAGLEEQDLEGAEPLELFKRWFEDAQRSGLVEEPEAMCLSTGLPTGEVSSRFVLLKKIEPAAGLVFFTNYDSRKAQEIRRNAHVSAAFYWGPLHQQIRICGKAEKIPEEESQRYFDGRPLGSRIGAWASPQSSPLESRAHLLDLVASHEARFELQPGSIDRHPPSQQDLDTLIPRPDYWGGFRIIPNEIEFWVGRPNRLHDRFVFKRSLDGLAPSSPWTRQRLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QRB7</accession>
    <name>A0A5B0QRB7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="8">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">YUH1_2</name>
        <name evidence="10" type="ORF">PGT21_036680</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">21-0</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the peptidase C12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="8"/>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QRB7"/>
    <dbReference type="OrthoDB" id="427186at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09616">
        <property type="entry name" value="Peptidase_C12_UCH_L1_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000006">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057254">
        <property type="entry name" value="UCH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF17">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00140">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="9">
        <location>
            <begin position="2"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="7">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAA1115455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="242" mass="26446" checksum="FDBD569FFDD9E3D0" modified="2019-11-13" version="1">MRWLPLESNPEVMNAWSSALGLSTDQVSFTDVYGLDAELLAMVPKPVYAVLMLFPISKEYEAARAAEHARIVDQAGGEVKSDERLIFIKQTISNACGTIGLLHALANNSEIPITEGPLTKFLEQCRAKSPSERAQLLEDDCAIADVHADQAQSGQSKVPSSDEEVNLHFVCFVKKIAQPDRPEAEPARLVELDGRKAFPIDHGPIAPSQDLLEAVVPVVKQFIQLSQDNVNFNLIALCQNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-02-22" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QRF6</accession>
    <name>A0A5B0QRF6_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_036896</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QRF6"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1115495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="118" mass="13685" checksum="11583C4350E777BC" modified="2019-11-13" version="1">MALSTSQSILEYTRHHNLTCDPDWTFEFAEDLVIEFSIAFKLAPPTQSLHEDIKHTWSSLDLITSQNGKENYKQLESLDSWLIPQGRLAYAKVVRDLVKRYKHLDLADPDWWGGQPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QUL6</accession>
    <name>A0A5B0QUL6_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_050138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000003">
        <property type="protein sequence ID" value="KAA1116414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QUL6"/>
    <dbReference type="OrthoDB" id="2513437at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1116414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="14059" checksum="B97917321A365B26" modified="2019-11-13" version="1">MDTFSRKDAMDDKGISAVPKLTGENYSIWESKMHYFLDSRQLIDVCLHEQPLPISDETKAKHSCAMFHLSSVVDDSIYNSIFKLSSNLTPFSVWSTLKTKYASKSIFSLCKVWRLWDTIHCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QW40</accession>
    <name>A0A5B0QW40_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription factor spt8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SPT8_3</name>
        <name evidence="3" type="ORF">PGT21_013851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QW40"/>
    <dbReference type="OrthoDB" id="10260946at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002344">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057544">
        <property type="entry name" value="Beta-prop_SPT8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23798">
        <property type="entry name" value="Beta-prop_SPT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor spt8 beta-propeller" evidence="2">
        <location>
            <begin position="139"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1117541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="347" mass="36795" checksum="44FCA0C022AA7314" modified="2019-11-13" version="1">MKTVESVKVSQAPSQEIVLEKTVEPTASSSPDRPLASDIVGEEDSEEDGSLFGGADNSGSEGEGSPEASVPLATLNPERGKGGGNGGGLILPTASGSEKPVPTKPAAPPLILPTVATSKPRNVVPVKPVLPATNLTQVPSVLDSSLPVINDDVLMTSGIDGQVYLWDRRVSGSGSHGYVRKLDLPKSTSPWTASASWSIDGRSVFVGRRNHSVDLYDLRFLRPSSPIFNPDAIQRSIKLPPSSGPVSCVKPWPDGNHLLCASFDNLRLYNLNADLHPTSLSSVRKSTHQPPKIPFKIIPGHSSGVVSQMCVTPSFRFLITASGDRGWQNTSNESVVIHEIRAPPPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0QYG8</accession>
    <name>A0A5B0QYG8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_023732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000002">
        <property type="protein sequence ID" value="KAA1117804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0QYG8"/>
    <dbReference type="OrthoDB" id="2497203at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1117804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="259" mass="28525" checksum="E993EB516982F512" modified="2019-11-13" version="1">MRFFSRMLAPSRDQSAPPKHSQVAPPTLHHHLQRPTRILRSPNLEIQDLLAAEPARVSLTPLPYDANGLKLVSRIALKWKSRFGQSPGPVPTASQSDLGHGCPLQPTLTQQSSPDQRAKLRRHSHLPPGPRMTGAKSFSDARPLRDLSPAAPPLKPKTSSIFGAKKKGISQISRPRLLTEDPWNTQISLHRSPSQHRRNIPVDMDFACFSPSNPQDISIDSNDYAASTLDLLHFYREVHGYSPASSSLGASCSPRLGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0R2D4</accession>
    <name>A0A5B0R2D4_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HPP transmembrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGT21_031860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">21-0</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000001">
        <property type="protein sequence ID" value="KAA1119687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0R2D4"/>
    <dbReference type="OrthoDB" id="2016548at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007065">
        <property type="entry name" value="HPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058581">
        <property type="entry name" value="TM_HPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33741">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0269096-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33741:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0269096-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04982">
        <property type="entry name" value="TM_HPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="HPP transmembrane region" evidence="3">
        <location>
            <begin position="127"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1119687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <sequence length="366" mass="39935" checksum="51AAF9FF542728F6" modified="2019-11-13" version="1">MSTPSKQLDDFSSGDESVNDNKHSPSTSSRSLRAGSTASSETDSSHSKDSPVTSASKSLGELESQLSTLGRPSARTIRSLEELANFDVRPKLPACISRFLGYRKDGICRPIWMLQWLDTFHVPLVVEEYFFACFCTNFGLISVIAINTMTPFSSLPIPISLGFVGALSIILYLTPHSPAAAPRNVFLSHLFASMIATVIAKLFLSDAHKTILIAGQMTTQTVWVWACVAVTSTLIFQKIFGLVHPPAGGTALIGTIQPAFIRIGWRYVGFVMSSVLCMLGVALLWGNVGRRAYPDFWFIQPTSPPSNAFQTNPKDSDSVESCSSSRRMSEVSYQSCEDSKDNLTTSSRFNQDISSEKSLSLSIRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RB66</accession>
    <name>A0A5B0RB66_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGT21_005605</name>
        <name evidence="7" type="ORF">PGTUg99_013531</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">21-0</strain>
            <strain evidence="7 9">Ug99</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000214">
        <property type="protein sequence ID" value="KAA1122917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RB66"/>
    <dbReference type="OrthoDB" id="2498727at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAA1083737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAA1122917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="531" mass="60187" checksum="95223B0F8544E190" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RGH8</accession>
    <name>A0A5B0RGH8_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_010407</name>
        <name evidence="3" type="ORF">PGTUg99_036083</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000029">
        <property type="protein sequence ID" value="KAA1107330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000203">
        <property type="protein sequence ID" value="KAA1124860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RGH8"/>
    <dbReference type="OrthoDB" id="10301364at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1107330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1124860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="193" mass="21115" checksum="FF5B5C2C6FD0B0B5" modified="2019-11-13" version="1">MIFTWGCSQGSGEGRPKAGPQRQAVRVSPCPRPDGSSSEASLIAGSLVGSVRAWFNSTCRRFFLSGRWLTRELTSALLEGLLSHKILCTSQRRARARLKQVSFPQVWTRSPVFWTNSLVLDGPARYPATRLGTQPPASVPSHPPRYPWKLLGRASTRLASIPAEKNSEELPRVYRSGASLRPTALGTSACGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RIG4</accession>
    <name>A0A5B0RIG4_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_013256</name>
        <name evidence="4" type="ORF">PGTUg99_008398</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000040">
        <property type="protein sequence ID" value="KAA1105612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000182">
        <property type="protein sequence ID" value="KAA1125169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RIG4"/>
    <dbReference type="OrthoDB" id="2506830at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1105612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1125169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="185" mass="20793" checksum="4214486DBA890A11" modified="2019-11-13" version="1">MLPISSLARIQKFVAPWTQIMRARLEPLVIFLSFTIIYLSSIAEGYLITTTINQIDDVRAAISKQDINNFHVYTPKLLNQYLFPSGQIDVNAVLALIAARLSYIPFFNEVKVDQVGSAGGCTDSSSKLSSHSTDLSSSTNKSVKAHLHHRASSFKKNLCRKFCSKKKKEEEEEDKERVKKEGKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RMY2</accession>
    <name>A0A5B0RMY2_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_030805</name>
        <name evidence="3" type="ORF">PGTUg99_033220</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000157">
        <property type="protein sequence ID" value="KAA1075189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000171">
        <property type="protein sequence ID" value="KAA1126692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RMY2"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138184" evidence="1">
        <location>
            <begin position="19"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1075189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1126692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="92" mass="10461" checksum="3DCFE38A76842CDC" modified="2019-11-13" version="1">MRFTFLVTALAVCTGVFANPETQHAEAQATTQAGQTAQLAQTAENWRRPYPGFYNYPPHPVYAPYPGYTPYPQYGPRPGGYYGRNPYPQHFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RP86</accession>
    <name>A0A5B0RP86_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGT21_017764</name>
        <name evidence="2" type="ORF">PGTUg99_031907</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">21-0</strain>
            <strain evidence="2 4">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000145">
        <property type="protein sequence ID" value="KAA1076755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000170">
        <property type="protein sequence ID" value="KAA1126925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RP86"/>
    <dbReference type="OrthoDB" id="2509878at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAA1076755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1126925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="217" mass="25147" checksum="3BB858536597630A" modified="2019-11-13" version="1">MDGSYGERPKVQELRKQADLIIQGLEQLRLRGNDFFLWPSRTSDPQIDITIEQLRLKEELLSRLASVLLPQLRLQCTSLSHQYAELSYVPEAPGSTVKLISEIQANLHLNLAQIIQTINQLIPYEIPAPENRTHDQDFNRLKIYRIYRLNHIIRNDLKIHLESLFRKSIQILREFGISSHEEKHTNTMESILIDISEAIDLAIGYLKGSELSLVWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RPZ8</accession>
    <name>A0A5B0RPZ8_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="2">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TRP4_2</name>
        <name evidence="11" type="ORF">PGT21_034537</name>
        <name evidence="12" type="ORF">PGTUg99_036821</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">21-0</strain>
            <strain evidence="12 14">Ug99</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="2"/>
    <dbReference type="EMBL" id="VSWC01000014">
        <property type="protein sequence ID" value="KAA1115270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000169">
        <property type="protein sequence ID" value="KAA1127449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RPZ8"/>
    <dbReference type="OrthoDB" id="427800at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1030.10:FF:000002">
        <property type="entry name" value="Anthranilate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glycosyl transferase family 3" evidence="10">
        <location>
            <begin position="118"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAA1115270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAA1127449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="398" mass="42818" checksum="9CFC53D5CC3D5808" modified="2019-11-13" version="1">MSSDFQPVLRKIVEFCQQYTQPQGHQNQPVNGNAAQNGLKHDHLPILADLQTTIQILADEKTSVPIQFAGLLTALTVSGLDRNERVIKTFVQEILSRWNLGAGLVDRRPPEHDPSVNPICDIVGTGGDGFNTFNVSTTAAIVAAGAGVKVCKHGNRASSSASGSADLLMALGIPLDRLDPSQVAQLIQDPELDFAFLFSAKFYPIFARLSPIRKSLGFPTIFNLLGPLLNPVRPDRLIIGVTKPELGPIFCRVLQLCGSSGSWVVCSEEGLDEISTAGETSLWKLESDGSIIHQRISPTQTFGLPSHSLDLLTGESAQENLQMLHDLLDGNFSTKKHEAILDFVLMNAAALIVLSGKTDDFKEGVKLARDSLVSGKAKATINSFKDKANSIALDGSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2023-09-13" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0RV43</accession>
    <name>A0A5B0RV43_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_021626</name>
        <name evidence="4" type="ORF">PGTUg99_031604</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000079">
        <property type="protein sequence ID" value="KAA1094445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000138">
        <property type="protein sequence ID" value="KAA1129149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0RV43"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036138227" description="Phorbol-ester/DAG-type domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1094445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1129149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17343" checksum="38BE9A24C1DF76AA" modified="2019-11-13" version="1">MLSIYILVAAMLSQVLAETAIIHPSDQIASVEKNAQMKSGHQLTKRMESIRAKLSGKCGKCHEELDSNSDKYKCSECDVYYHTRCGSGQDCPTCKKQRQLQEREVKKAQYEQYQNEIQYGRESSSRRYSDEDRHGSQERDDHYSQNRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0S6C7</accession>
    <name>A0A5B0S6C7_PUCGR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">uS12 prolyl 3,4-dihydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGT21_002529</name>
        <name evidence="16" type="ORF">PGTUg99_031468</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">21-0</strain>
            <strain evidence="16 18">Ug99</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3,4-dihydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54160"/>
            <dbReference type="Rhea" id="RHEA-COMP:13817"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
            <dbReference type="ChEBI" id="CHEBI:138052"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[ribosomal protein uS12]-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54156"/>
            <dbReference type="Rhea" id="RHEA-COMP:13816"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000119">
        <property type="protein sequence ID" value="KAA1082417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000071">
        <property type="protein sequence ID" value="KAA1133691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0S6C7"/>
    <dbReference type="OrthoDB" id="430522at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031543">
        <property type="term" value="F:peptidyl-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009896">
        <property type="term" value="P:positive regulation of catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000014">
        <property type="entry name" value="Prolyl 3,4-dihydroxylase TPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.20:FF:000003">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.3, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.20">
        <property type="entry name" value="Oxoglutarate/iron-dependent oxygenase, C-terminal degradation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019601">
        <property type="entry name" value="Oxoglutarate/Fe-dep_Oase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043044">
        <property type="entry name" value="TPA1/Ofd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039558">
        <property type="entry name" value="TPA1/OFD1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051842">
        <property type="entry name" value="uS12_prolyl_hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117:SF0">
        <property type="entry name" value="PROLYL 3-HYDROXYLASE OGFOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13661">
        <property type="entry name" value="2OG-FeII_Oxy_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10637">
        <property type="entry name" value="Ofd1_CTDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="157"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="517"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAA1082417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAA1133691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="603" mass="67605" checksum="1C4843F091651934" modified="2019-11-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SF77</accession>
    <name>A0A5B0SF77_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_007636</name>
        <name evidence="4" type="ORF">PGTUg99_000397</name>
        <name evidence="2" type="ORF">PGTUg99_030068</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="4 6">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000508">
        <property type="protein sequence ID" value="KAA1067065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000118">
        <property type="protein sequence ID" value="KAA1083808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000024">
        <property type="protein sequence ID" value="KAA1136786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SF77"/>
    <dbReference type="OrthoDB" id="2500318at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1067065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1083808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAA1136786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="256" mass="28400" checksum="74B4DA77E61D775C" modified="2019-11-13" version="1">MRVGKRINPEYFVSFILPIVLILPLAFVSFYLSVINHPSTMKCTTTAIFFALSLSAVTATLHTRCYDYFLAKDGCVFSAGEESQRCAAPKKRPSQPVKAFAMNPEVSKAKRSQIPGLERRYDDTQPSFYVSGGNGTCGHYDTNTQLGVCLWNGAEQDNPTAETAGWLNGDQTANCGKQVYIQRKGHPETVQYVNVLDGCYFEAKTPEPGCFEIGITLALFNKLNPTSQEQQDGYIYDGISWDFNKPNNTATDWSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0SMP5</accession>
    <name>A0A5B0SMP5_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGT21_031982</name>
        <name evidence="3" type="ORF">PGTUg99_036568</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">21-0</strain>
            <strain evidence="3 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VSWC01000027">
        <property type="protein sequence ID" value="KAA1110804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VDEP01000001">
        <property type="protein sequence ID" value="KAA1139140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0SMP5"/>
    <dbReference type="OrthoDB" id="2505281at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAA1110804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAA1139140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000324748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000325313"/>
        </source>
    </evidence>
    <sequence length="137" mass="15288" checksum="78F52AEE69C399BC" modified="2019-11-13" version="1">MTTLITCTHHGLVGPTGTEQDELQRLESDVRSFELSLIKALMDPLDQQSLAAVYLSSMKLLPYINSPSCDTRGPRSAHRKYLIDRVFLLLLALHHPDCSLGKSKQRFKPSPSSKPPSNSPLPRDWLLGILAQAVRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-11-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A5B0LLM0</accession>
    <name>A0A5B0LLM0_PUCGR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGT21_006187</name>
        <name evidence="2" type="ORF">PGTUg99_004012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici</name>
        <dbReference type="NCBI Taxonomy" id="56615"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2019-05" db="EMBL/GenBank/DDBJ databases">
            <title>Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridization.</title>
            <authorList>
                <person name="Li F."/>
                <person name="Upadhyaya N.M."/>
                <person name="Sperschneider J."/>
                <person name="Matny O."/>
                <person name="Nguyen-Phuc H."/>
                <person name="Mago R."/>
                <person name="Raley C."/>
                <person name="Miller M.E."/>
                <person name="Silverstein K.A.T."/>
                <person name="Henningsen E."/>
                <person name="Hirsch C.D."/>
                <person name="Visser B."/>
                <person name="Pretorius Z.A."/>
                <person name="Steffenson B.J."/>
                <person name="Schwessinger B."/>
                <person name="Dodds P.N."/>
                <person name="Figueroa M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">21-0</strain>
            <strain evidence="2 5">Ug99</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="VDEP01000517">
        <property type="protein sequence ID" value="KAA1064004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="VSWC01000197">
        <property type="protein sequence ID" value="KAA1064518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A5B0LLM0"/>
    <dbReference type="OrthoDB" id="10283187at2759"/>
    <dbReference type="Proteomes" id="UP000324748">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000325313">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </l